PMID- 10036181
OWN - NLM
STAT- MEDLINE
DCOM- 19990505
LR  - 20181201
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 56
IP  - 1
DP  - 1999 Feb 15
TI  - Discovery of three novel orphan G-protein-coupled receptors.
PG  - 12-21
AB  - We have discovered three novel human genes, GPR34, GPR44, and GPR45, encoding
      family A G-protein-coupled receptors (GPCRs). The receptor encoded by GPR34 is
      most similar to the P2Y receptor subfamily, while the receptor encoded by GPR44
      is most similar to chemoattractant receptors. The receptor encoded by GPR45 is
      the mammalian orthologue of a putative lysophosphatidic acid receptor from
      Xenopus laevis. Partial sequence of GPR34 was discovered during a search of the
      GenBank database of expressed sequence tags (ESTs). This sequence information was
      used both to isolate the full-length translational open reading frame from a
      human genomic library and to assemble a contig from additional GPR34 EST cDNAs.
      Northern blot and in situ hybridization analyses revealed GPR34 mRNA transcripts 
      in several human and rat brain regions. Also, we used polymerase chain reaction
      (PCR) to amplify human genomic DNA using degenerate oligonucleotides designed
      from sequences encoding transmembrane domains 3 and 7 of opioid and somatostatin 
      receptors. Two PCR products partially encoding novel GPCRs, named GPR44 and
      GPR45, were discovered and used to isolate the full-length translational open
      reading frames from a human genomic library. Both GPR44 and GPR45 are expressed
      in the central nervous system and periphery. For chromosomal localization,
      fluorescence in situ hybridization analysis was performed to assign GPR34 to
      chromosomes 4p12 and Xp11. 3, GPR44 to chromosome 11q12-q13.3, and GPR45 to
      chromosome 2q11. 1-q12.
CI  - Copyright 1999 Academic Press.
FAU - Marchese, A
AU  - Marchese A
AD  - Department of Pharmacology, Department of Medicine, University of Toronto,
      Medical Sciences Building, Toronto, Ontario, M5S 1A8, Canada.
FAU - Sawzdargo, M
AU  - Sawzdargo M
FAU - Nguyen, T
AU  - Nguyen T
FAU - Cheng, R
AU  - Cheng R
FAU - Heng, H H
AU  - Heng HH
FAU - Nowak, T
AU  - Nowak T
FAU - Im, D S
AU  - Im DS
FAU - Lynch, K R
AU  - Lynch KR
FAU - George, S R
AU  - George SR
FAU - O'dowd, B F
AU  - O'dowd BF
LA  - eng
SI  - GENBANK/AF118265
SI  - GENBANK/AF118266
SI  - GENBANK/AF118670
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (G-protein-coupled receptor 34)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Lysophosphatidic Acid)
RN  - 0 (Receptors, Lysophospholipid)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (Receptors, Purinergic P2)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - XZF106QU24 (prostaglandin D2 receptor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 2/genetics
MH  - Chromosomes, Human, Pair 4/genetics
MH  - Cloning, Molecular
MH  - GTP-Binding Proteins/*genetics
MH  - Humans
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Rats
MH  - Receptors, Cell Surface/*genetics
MH  - Receptors, Chemokine/*genetics
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Immunologic
MH  - Receptors, Lysophosphatidic Acid
MH  - Receptors, Lysophospholipid
MH  - Receptors, Prostaglandin
MH  - Receptors, Purinergic P2/*genetics
MH  - Sequence Analysis
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - X Chromosome/genetics
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - S0888-7543(98)95655-5 [pii]
AID - 10.1006/geno.1998.5655 [doi]
PST - ppublish
SO  - Genomics. 1999 Feb 15;56(1):12-21. doi: 10.1006/geno.1998.5655.