PMID- 10030676
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 17
IP  - 26
DP  - 1998 Dec 31
TI  - Isolation and characterization of a human homologue of the latrophilin gene from 
      a region of 1p31.1 implicated in breast cancer.
PG  - 3513-9
AB  - We have identified a region of chromosome 1p31.1 that shows high frequency loss
      of heterozygosity (LOH) in human breast cancer. This region forms part of a 7 Mb 
      YAC/BAC contig. In order to identify candidate sequences, mutation of which might
      contribute to the development of disease, we have carried out mapping studies of 
      ESTs localized to 1p31.1. This analysis, coupled with library screening and a
      modified 5' RACE-PCR strategy, resulted in the identification and
      characterization of a novel gene (LPHH1) which is located adjacent to the
      smallest region of overlapping loss (SRO) seen in tumours. The 4209 bp open
      reading frame of the 7 kb LPHH1 transcript encodes a peptide which shows
      approximately 65% identity to rat latrophilin, a G-coupled, seven span
      transmembrane protein, which binds alpha-latrotoxin. In the human sequence,
      whilst conservation of the transmembrane domain is high, the intra- and
      extracellular domains show two regions of variable structure, which are
      presumably generated by alternative splicing. Surprisingly, while expression of
      the rat gene is tightly restricted to neurological and perhaps some endocrine
      cells, the human sequence appears to be expressed very widely in all normal
      tissues tested. Northern and RT-PCR analysis of a panel of tumour cell lines
      showed that LPHH1 expression was variable, apparently elevated in some lines and 
      absent or markedly reduced in others. Furthermore, characterization of the range 
      of transcripts encoded in a breast tumour cell line, compared to normal breast,
      suggested that gene product variability was higher in the tumour.
FAU - White, G R
AU  - White GR
AD  - CRC Section of Molecular Genetics, Paterson Institute for Cancer Research,
      Christie Hospital NHS Trust, Manchester, UK.
FAU - Varley, J M
AU  - Varley JM
FAU - Heighway, J
AU  - Heighway J
LA  - eng
SI  - GENBANK/AJ131581
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Complementary)
RN  - 0 (LPHN2 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Peptide)
RN  - 0 (alpha-latrotoxin receptor)
SB  - IM
EIN - Oncogene 1999 Mar 25;18(12):2167
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Breast Neoplasms/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Rats
MH  - Receptors, G-Protein-Coupled
MH  - Receptors, Peptide/*genetics
MH  - Reference Values
MH  - Sequence Analysis
MH  - Tumor Cells, Cultured
EDAT- 1999/02/25 00:00
MHDA- 1999/02/25 00:01
CRDT- 1999/02/25 00:00
PHST- 1999/02/25 00:00 [pubmed]
PHST- 1999/02/25 00:01 [medline]
PHST- 1999/02/25 00:00 [entrez]
AID - 10.1038/sj.onc.1202487 [doi]
PST - ppublish
SO  - Oncogene. 1998 Dec 31;17(26):3513-9. doi: 10.1038/sj.onc.1202487.