PMID- 10030672
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20161124
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 17
IP  - 26
DP  - 1998 Dec 31
TI  - Identification of a human HECT family protein with homology to the Drosophila
      tumor suppressor gene hyperplastic discs.
PG  - 3479-91
AB  - Use of the differential display technique to isolate progestin-regulated genes in
      T-47D human breast cancer cells led to identification of a novel gene, EDD. The
      cDNA sequence contains a 2799 amino acid open reading frame sharing 40% identity 
      with the predicted 2894 amino acid product of the Drosophila melanogaster tumor
      suppressor gene hyperplastic discs, while the carboxy-terminal 889 amino acids
      show 96% identity to a rat 100 kDa HECT domain protein. EDD mRNA was
      progestin-induced in T-47D cells and was highly abundant in testes and expressed 
      at moderately high levels in other tissues, suggesting a broad role for EDD.
      Anti-EDD antibodies immunoprecipitated an approximately 300 kDa protein from
      T-47D cell lysates. HECT family proteins function as E3 ubiquitin-protein
      ligases, targeting specific proteins for ubiquitin-mediated proteolysis. EDD is
      likely to function as an E3 as in vitro translated protein bound ubiquitin
      reversibly through a conserved HECT domain cysteine residue. EDD was localized by
      FISH to chromosome 8q22, a locus disrupted in a variety of cancers. Given the
      homology between EDD and the hyperplastic discs protein, which is required for
      control of imaginal disc growth in Drosophila, EDD potentially has a role in
      regulation of cell proliferation or differentiation.
FAU - Callaghan, M J
AU  - Callaghan MJ
AD  - Cancer Research Program, Garvan Institute of Medical Research, St Vincent's
      Hospital, Sydney, NSW, Australia.
FAU - Russell, A J
AU  - Russell AJ
FAU - Woollatt, E
AU  - Woollatt E
FAU - Sutherland, G R
AU  - Sutherland GR
FAU - Sutherland, R L
AU  - Sutherland RL
FAU - Watts, C K
AU  - Watts CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Helminth Proteins)
RN  - 0 (Pregnenediones)
RN  - 0 (Proteins)
RN  - 0 (Ubiquitins)
RN  - 24320-06-7 (16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione)
RN  - EC 2.3.2.26 (UBR5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - *Chromosomes, Human, Pair 8
MH  - Cloning, Molecular
MH  - Drosophila melanogaster/*genetics
MH  - Genetic Techniques
MH  - Helminth Proteins/genetics
MH  - Humans
MH  - Ligases/genetics/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - *Peptide Synthases
MH  - Pregnenediones/pharmacology
MH  - Proteins/*genetics/*metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitins/metabolism
EDAT- 1999/02/25 00:00
MHDA- 1999/02/25 00:01
CRDT- 1999/02/25 00:00
PHST- 1999/02/25 00:00 [pubmed]
PHST- 1999/02/25 00:01 [medline]
PHST- 1999/02/25 00:00 [entrez]
AID - 10.1038/sj.onc.1202249 [doi]
PST - ppublish
SO  - Oncogene. 1998 Dec 31;17(26):3479-91. doi: 10.1038/sj.onc.1202249.