PMID- 10028916
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20191102
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 25
IP  - 4
DP  - 1999 Feb 15
TI  - TGF-beta1-dependent differential expression of a rat homolog for latent TGF-beta 
      binding protein in astrocytes and C6 glioma cells.
PG  - 332-42
AB  - Transforming growth factor-beta1 (TGF-beta1) is widely recognized for its
      multiple roles in development, cellular maintenance, and protection against
      injury. In the brain, TGF-beta1 upregulation in microglia/macrophages is a
      predominant response to lesion and during pathology. However, the precise
      functions of TGF-beta1 in this context are still enigmatic. The present study
      investigates changes in astroglial gene expression as a major target of TGF-beta1
      signaling in the brain. Differential display reverse transcription-polymerase
      chain reaction (DDRT-PCR) was used to identify several gene fragments
      differentially regulated by TGF-beta1 in rat astrocytes and C6 glioma cells.
      Among the cDNAs regulated by TGF-beta1 in C6 cells two cDNAs showed homology to
      alpha-tropomyosin and glycerol-3-phosphate dehydrogenase, respectively. Cloning
      of a full length cDNA corresponding to a differentially regulated gene fragment
      revealed close homology to latent TGF-beta binding protein (LTBP)-2. Data using
      antisense LTBP-2 oligonucleotides to decrease LTBP-2 expression suggest that
      LTBP-2 functions to activate TGF-beta. Therefore, it is likely that upregulation 
      of the rat LTBP-2 homolog mRNA in C6 cells and cortical astrocytes by TGF-1 might
      lead to self-activation and exaggeration of TGF-beta signaling. These data will
      extend our current understanding of TGF-beta1 functioning on lesion-related
      features of glial cells.
FAU - Krohn, K
AU  - Krohn K
AD  - Department of Anatomy and Cell Biology III, University of Heidelberg, Germany.
      krok@server3.medizin.uni-leipzig.de
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Latent TGF-beta Binding Proteins)
RN  - 0 (Ltbp2 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/drug effects/metabolism
MH  - Carrier Proteins/*drug effects/genetics/metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Glioma/genetics/metabolism
MH  - Latent TGF-beta Binding Proteins
MH  - Neuroglia/drug effects/metabolism
MH  - RNA, Messenger/drug effects/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Cells, Cultured/drug effects
MH  - Up-Regulation
EDAT- 1999/02/24 00:00
MHDA- 1999/02/24 00:01
CRDT- 1999/02/24 00:00
PHST- 1999/02/24 00:00 [pubmed]
PHST- 1999/02/24 00:01 [medline]
PHST- 1999/02/24 00:00 [entrez]
AID - 10.1002/(SICI)1098-1136(19990215)25:4<332::AID-GLIA3>3.0.CO;2-1 [pii]
AID - 10.1002/(sici)1098-1136(19990215)25:4<332::aid-glia3>3.0.co;2-1 [doi]
PST - ppublish
SO  - Glia. 1999 Feb 15;25(4):332-42. doi:
      10.1002/(sici)1098-1136(19990215)25:4<332::aid-glia3>3.0.co;2-1.