PMID- 1002715
OWN - NLM
STAT- MEDLINE
DCOM- 19770216
LR  - 20061115
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 251
IP  - 24
DP  - 1976 Dec 25
TI  - Isolation of eukaryotic ribosomal proteins. Purification and characterization of 
      the 60 S ribosomal subunit proteins L4, L5, L7, L9, L11, L12, L13, L21, L22, L23,
      L26, L27, L30, L33, L35', L37, and L39.
PG  - 7940-6
AB  - The proteins of the large subunit of rat liver ribosomes were separated into
      seven groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5.
      Seventeen proteins (L4, L5, L7, L9, L11, L12, L13, L21, L22, L23, L26, L27, L30, 
      L33, L35', L37, and L39) were isolated from three of the groups (B60, D60, G60)
      by ion exchange chromatography on carboxymethylcellulose and by filtration
      through Sephadex. The amount of protein obtained varied from 0.5 to 15 mg. Eight 
      of the proteins (L9, L11, L13, L21, L22, L35', L37 and L39) had no detectable
      contamination; the impurities in the others were no greater than 9%. The
      molecular weight of the proteins was estimated by polyacrylamide gel
      electrophoresis in sodium dodecyl sulfate; the amino acid composition was
      determined.
FAU - Tsurugi, K
AU  - Tsurugi K
FAU - Collatz, E
AU  - Collatz E
FAU - Wool, E G
AU  - Wool EG
FAU - Lin, A
AU  - Lin A
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amino Acids)
RN  - 0 (Ribosomal Proteins)
SB  - IM
MH  - Amino Acids/analysis
MH  - Animals
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Liver/*analysis
MH  - Molecular Weight
MH  - Rats
MH  - *Ribosomal Proteins/isolation & purification
EDAT- 1976/12/25 00:00
MHDA- 1976/12/25 00:01
CRDT- 1976/12/25 00:00
PHST- 1976/12/25 00:00 [pubmed]
PHST- 1976/12/25 00:01 [medline]
PHST- 1976/12/25 00:00 [entrez]
PST - ppublish
SO  - J Biol Chem. 1976 Dec 25;251(24):7940-6.