PMID- 10026262
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20161124
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 6
DP  - 1999 Feb 9
TI  - DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer.
PG  - 1819-28
AB  - Ku antigen is composed of 70 and 82 kDa subunits (Ku70 and Ku80, respectively)
      that together bind with high affinity to ends of double-stranded DNA and other
      DNA structures in vitro. When bound to DNA, the Ku 70/80 heterodimer enhances the
      kinase activity of the catalytic subunit of the DNA-dependent protein kinase,
      DNA-PKcs. Ku and DNA-PKcs are required for V(D)J recombination and DNA
      double-strand break repair in vivo and may also play a role in regulation of
      transcription. Ku is phosphorylated by DNA-PKcs in vitro, and cells that lack
      DNA-PKcs are deficient in Ku phosphorylation in vitro, suggesting that Ku may be 
      a physiological target for DNA-PK. Here we have identified the sites of DNA-PK
      phosphorylation in human Ku protein. We find that Ku70 is phosphorylated at a
      single serine residue, serine 6, located in the putative transcriptional
      activation domain, and Ku80 is phosphorylated at serines 577 and 580 and at
      threonine 715. Interestingly, none of the phosphorylation sites identified in Ku 
      correspond to the serine-glutamine consensus for DNA-PK phosphorylation,
      consistent with previous reports that DNA-PK can recognize additional
      phosphorylation motifs.
FAU - Chan, D W
AU  - Chan DW
AD  - Department of Biological Sciences, University of Calgary, Canada.
FAU - Ye, R
AU  - Ye R
FAU - Veillette, C J
AU  - Veillette CJ
FAU - Lees-Miller, S P
AU  - Lees-Miller SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Antigens, Nuclear)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphopeptides)
RN  - EC 2.7.11.1 (DNA-Activated Protein Kinase)
RN  - EC 2.7.11.1 (PRKDC protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (XRCC5 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, human)
RN  - EC 3.6.4.12 (Xrcc6 protein, mouse)
RN  - EC 3.6.4.12 (Xrcc6 protein, rat)
RN  - EC 4.2.99.- (Ku Autoantigen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigens, Nuclear
MH  - Binding Sites
MH  - Cricetinae
MH  - *DNA Helicases
MH  - DNA-Activated Protein Kinase
MH  - DNA-Binding Proteins/*chemistry/isolation & purification/*metabolism
MH  - Dimerization
MH  - Humans
MH  - Ku Autoantigen
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Nuclear Proteins/*chemistry/isolation & purification/*metabolism
MH  - Phosphopeptides/isolation & purification/metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/chemistry/isolation & purification/*metabolism
MH  - Rats
MH  - Sequence Alignment
EDAT- 1999/02/23 00:00
MHDA- 1999/02/23 00:01
CRDT- 1999/02/23 00:00
PHST- 1999/02/23 00:00 [pubmed]
PHST- 1999/02/23 00:01 [medline]
PHST- 1999/02/23 00:00 [entrez]
AID - 10.1021/bi982584b [doi]
AID - bi982584b [pii]
PST - ppublish
SO  - Biochemistry. 1999 Feb 9;38(6):1819-28. doi: 10.1021/bi982584b.