PMID- 10026247
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20171116
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 6
DP  - 1999 Feb 9
TI  - Zinc-binding site of an S100 protein revealed. Two crystal structures of
      Ca2+-bound human psoriasin (S100A7) in the Zn2+-loaded and Zn2+-free states.
PG  - 1695-704
AB  - The crystal structure of human psoriasin (S100A7) in the native, calcium-bound
      form has been determined from two crystal forms of the protein crystallized with 
      and without divalent zinc. The overall structures of the dimeric protein closely 
      resemble the previously determined holmium-substituted structure. The structures 
      also reveal a zinc-binding site of the protein, which is formed by three
      histidines and an aspartate residue. Together, these residues coordinate the zinc
      ion in a way similar to the pattern seen in certain metalloproteases and in
      particular the collagenase family of proteins. Sequence comparison suggests that 
      this zinc site is present in a number of the remaining members of the S100
      family. The structure of S100A7 crystallized in the absence of zinc further shows
      that loss of zinc results in a reorganization of the adjacent empty and distorted
      EF-hand loop, causing it to resemble a calcium-loaded EF-hand.
FAU - Brodersen, D E
AU  - Brodersen DE
AD  - Macromolecular Crystallography, Institute of Molecular and Structural Biology,
      Aarhus University, Denmark.
FAU - Nyborg, J
AU  - Nyborg J
FAU - Kjeldgaard, M
AU  - Kjeldgaard M
LA  - eng
SI  - PDB/2PSR
SI  - PDB/3PSR
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (S100 Calcium Binding Protein A7)
RN  - 0 (S100 Proteins)
RN  - 0 (S100A7 protein, human)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Calcium/chemistry/*metabolism
MH  - Calcium-Binding Proteins/*chemistry/metabolism
MH  - Computer Simulation
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Metalloendopeptidases/chemistry
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Structure, Secondary
MH  - S100 Calcium Binding Protein A7
MH  - S100 Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Zinc/chemistry/*metabolism
EDAT- 1999/02/23 00:00
MHDA- 1999/02/23 00:01
CRDT- 1999/02/23 00:00
PHST- 1999/02/23 00:00 [pubmed]
PHST- 1999/02/23 00:01 [medline]
PHST- 1999/02/23 00:00 [entrez]
AID - 10.1021/bi982483d [doi]
AID - bi982483d [pii]
PST - ppublish
SO  - Biochemistry. 1999 Feb 9;38(6):1695-704. doi: 10.1021/bi982483d.