PMID- 10026238
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 47
IP  - 3
DP  - 1999 Mar
TI  - Three distinct classes of the alpha-subunit of the nuclear pore-targeting complex
      (importin-alpha) are differentially expressed in adult mouse tissues.
PG  - 363-72
AB  - The process of active nuclear protein transport is mediated by the nuclear
      localization signal (NLS). An NLS-containing karyophile forms a stable complex,
      termed the nuclear pore-targeting complex, to target nuclear pores. The
      alpha-subunit of the complex (importin-alpha) binds to the NLS and the
      beta-subunit (importin-beta) carries the alpha-subunit, bound to the NLS
      substrate, into the nucleus. To date, five mouse alpha-subunits have been
      identified and classified into three subfamilies (alpha-P, alpha-Q, and alpha-S).
      The expression of these alpha-subunits and the beta-subunit in various adult
      mouse tissues was examined by immunoblotting and immunohistochemistry using
      antibodies specific for each subfamily of the alpha-subunit or the beta-subunit. 
      The beta-subunit was found to be ubiquitously expressed, whereas each subfamily
      of the alpha-subunit showed a unique expression pattern in various tissues,
      especially in brain and testis. In brain, the expression of alpha-P was not
      observed, whereas alpha-S was significantly expressed in Purkinje cells, and
      pyramidal cells of the hippocampus and cerebral cortex. In testis, alpha-P was
      expressed predominantly in primary spermatocytes, whereas alpha-Q was found
      mainly in Leydig cells. Expression of alpha-S was detected in almost all cells in
      convoluted seminiferous tubules and Leydig cells to a similar extent. These
      results suggest that nuclear protein import may be controlled in a
      tissue-specific manner by alpha-subunit family proteins.
FAU - Kamei, Y
AU  - Kamei Y
AD  - Department of Biochemistry, Miyazaki Medical College, Miyazaki, Japan.
FAU - Yuba, S
AU  - Yuba S
FAU - Nakayama, T
AU  - Nakayama T
FAU - Yoneda, Y
AU  - Yoneda Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the
      Histochemistry Society
JID - 9815334
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - Karyopherins
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nuclear Proteins/*classification/immunology/*metabolism
MH  - Organ Specificity
MH  - Testis/*metabolism
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1177/002215549904700310 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1999 Mar;47(3):363-72. doi: 10.1177/002215549904700310.