PMID- 10026224
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Characterization of ZO-2 as a MAGUK family member associated with tight as well
      as adherens junctions with a binding affinity to occludin and alpha catenin.
PG  - 5981-6
AB  - ZO-2, a member of the MAGUK family, was thought to be specific for tight
      junctions (TJs) in contrast to ZO-1, another MAGUK family member, which is
      localized at TJs and adherens junctions (AJs) in epithelial and nonepithelial
      cells, respectively. Mouse ZO-2 cDNA was isolated, and a specific polyclonal
      antibody was generated using corresponding synthetic peptides as antigens.
      Immunofluorescence microscopy with this polyclonal antibody revealed that,
      similarly to ZO-1, in addition to TJs in epithelial cells, ZO-2 was also
      concentrated at AJs in nonepithelial cells such as fibroblasts and cardiac muscle
      cells lacking TJs. When NH2-terminal dlg-like and COOH-terminal non-dlg-like
      domains of ZO-2 (N-ZO-2 and C-ZO-2, respectively) were separately introduced into
      cultured cells, N-ZO-2 was colocalized with endogenous ZO-1/ZO-2, i.e. at TJs in 
      epithelial cells and at AJs in non-epithelial cells, whereas C-ZO-2 was
      distributed along actin filaments. Consistently, occludin as well as alpha
      catenin directly bound to N-ZO-2 as well as the NH2-terminal dlg-like portion of 
      ZO-1 (N-ZO-1) in vitro. Furthermore, immunoprecipitation experiments revealed
      that the second PDZ domain of ZO-2 was directly associated with N-ZO-1. These
      findings indicated that ZO-2 forms a complex with ZO-1/occludin or ZO-1/alpha
      catenin to establish TJ or AJ domains, respectively.
FAU - Itoh, M
AU  - Itoh M
AD  - Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku,
      Kyoto 606, Japan.
FAU - Morita, K
AU  - Morita K
FAU - Tsukita, S
AU  - Tsukita S
LA  - eng
SI  - GENBANK/AF113005
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antibodies)
RN  - 0 (Ctnna1 protein, mouse)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Occludin)
RN  - 0 (Ocln protein, mouse)
RN  - 0 (Phosphoproteins)
RN  - 0 (Tjp1 protein, mouse)
RN  - 0 (Tjp2 protein, mouse)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 0 (Zonula Occludens-2 Protein)
RN  - 0 (alpha Catenin)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/*metabolism
MH  - DNA, Complementary
MH  - Membrane Proteins/genetics/immunology/*metabolism
MH  - Mice
MH  - Occludin
MH  - Phosphoproteins/metabolism
MH  - Protein Binding
MH  - Subcellular Fractions/metabolism
MH  - Tight Junctions/*metabolism
MH  - Zonula Occludens-1 Protein
MH  - Zonula Occludens-2 Protein
MH  - alpha Catenin
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5981 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5981-6. doi: 10.1074/jbc.274.9.5981.