PMID- 10026212
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Human geranylgeranyl diphosphate synthase. cDNA cloning and expression.
PG  - 5888-94
AB  - Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of 
      GGPP, which is an important molecule responsible for the C20-prenylated protein
      biosynthesis and for the regulation of a nuclear hormone receptor (LXR.RXR). The 
      human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence
      identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The 
      GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240
      nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as 
      an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts
      with an antibody directed against bovine brain GGPPSase, which differs
      immunochemically from bovine brain FPPSase. Northern blot analysis indicates the 
      presence of two forms of the mRNA.
FAU - Kuzuguchi, T
AU  - Kuzuguchi T
AD  - Institute for Chemical Reaction Science, Tohoku University, 2-1-1 Katahira,
      Aoba-ku, Sendai, 980-8577, Japan.
FAU - Morita, Y
AU  - Morita Y
FAU - Sagami, I
AU  - Sagami I
FAU - Sagami, H
AU  - Sagami H
FAU - Ogura, K
AU  - Ogura K
LA  - eng
SI  - GENBANK/AB017971
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - EC 2.5.- (Alkyl and Aryl Transferases)
RN  - EC 2.5.1.29 (Farnesyltranstransferase)
SB  - IM
MH  - Alkyl and Aryl Transferases/*genetics/immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/enzymology
MH  - Cattle
MH  - Cloning, Molecular
MH  - Cross Reactions
MH  - DNA
MH  - DNA, Complementary
MH  - Escherichia coli/genetics
MH  - Farnesyltranstransferase
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - RNA, Messenger/genetics
MH  - Sequence Homology, Amino Acid
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5888 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5888-94. doi: 10.1074/jbc.274.9.5888.