PMID- 10026196
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Identification of three distinct receptor binding sites of murine interleukin-11.
PG  - 5755-61
AB  - Interleukin-11 (IL-11) is a member of the gp130 family of cytokines. These
      cytokines drive the assembly of multisubunit receptor complexes, all of which
      contain at least one molecule of the transmembrane signaling receptor gp130. A
      complex of IL-11 and the IL-11 receptor (IL-11R) has been shown to interact with 
      gp130, with high affinity, and to induce gp130- dependent signaling. In this
      study, we have identified residues crucial for the binding of murine IL-11
      (mIL-11) to both the IL-11R and gp130 by examining the activities of mIL-11
      mutants in receptor binding and cell proliferation assays. The location of these 
      residues, as predicted from structural studies and a model of IL-11, reveals that
      mIL-11 has three distinct receptor binding sites. These are structurally and
      functionally analogous to the previously defined receptor binding sites I, II,
      and III of interleukin-6 (IL-6). This supports the hypothesis that IL-11 signals 
      via the formation of a hexameric receptor complex and indicates that site III is 
      a generic feature of cytokines that signal via association with gp130.
FAU - Barton, V A
AU  - Barton VA
AD  - Cancer Research Campaign Growth Factor Group, Department of Biochemistry,
      University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom.
FAU - Hudson, K R
AU  - Hudson KR
FAU - Heath, J K
AU  - Heath JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (IL11RA protein, human)
RN  - 0 (Il11ra1 protein, mouse)
RN  - 0 (Interleukin-11)
RN  - 0 (Interleukin-11 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-11)
RN  - 0 (Recombinant Proteins)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Biotin/metabolism
MH  - Cell Line
MH  - Humans
MH  - Interleukin-11/chemistry/genetics/*metabolism
MH  - Interleukin-11 Receptor alpha Subunit
MH  - Mice
MH  - Mutagenesis
MH  - Protein Conformation
MH  - Receptors, Interleukin/chemistry/*metabolism
MH  - Receptors, Interleukin-11
MH  - Recombinant Proteins/chemistry/metabolism
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5755 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5755-61. doi: 10.1074/jbc.274.9.5755.