PMID- 10026184
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Mapping the functional domains of BRCA1. Interaction of the ring finger domains
      of BRCA1 and BARD1.
PG  - 5659-65
AB  - Breast cancer 1 (BRCA1) and BRCA1-associated RING domain 1 (BARD1) are
      multidomain proteins that interact in vivo via their N-terminal RING finger motif
      regions. To characterize functional aspects of the BRCA1/BARD1 interaction, we
      have defined the structural domains required for the interaction, as well as
      their oligomerization state, relative stability, and possible nucleic acid
      binding activity. We have found that the RING finger motifs do not themselves
      constitute stable structural domains but are instead part of larger domains
      comprising residues 1-109 of BRCA1 and residues 26-119 of BARD1. These domains
      exist as homodimers and preferentially form a stable heterodimer. Shorter BRCA1
      RING finger constructs do not interact with BARD1 or with longer BRCA1
      constructs, indicating that the heterodimeric and homodimer interactions are
      mediated by regions outside the canonical RING finger motif. Nucleic acid binding
      is a generally proposed function of RING finger domains. We show that neither the
      homodimers nor the heterodimer displays affinity for nucleic acids, indicating
      that the proposed roles of BRCA1 and BARD1 in DNA repair and/or transcriptional
      activation must be mediated either by other regions of the proteins or by
      additional cofactors.
FAU - Meza, J E
AU  - Meza JE
AD  - Departments of Genetics and Medical Genetics, University of Washington, Seattle, 
      Washington 98195-7742, USA.
FAU - Brzovic, P S
AU  - Brzovic PS
FAU - King, M C
AU  - King MC
FAU - Klevit, R E
AU  - Klevit RE
LA  - eng
GR  - R01 CA27632/CA/NCI NIH HHS/United States
GR  - R01 CA79953/CA/NCI NIH HHS/United States
GR  - R01 GM46701/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (BRCA1 Protein)
RN  - 0 (Biopolymers)
RN  - 0 (Carrier Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.3.2.27 (BARD1 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - BRCA1 Protein/*chemistry/metabolism
MH  - Biopolymers
MH  - Carrier Proteins/*chemistry/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Conformation
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Spectrometry, Fluorescence
MH  - *Tumor Suppressor Proteins
MH  - *Ubiquitin-Protein Ligases
MH  - Zinc Fingers
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5659 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5659-65. doi: 10.1074/jbc.274.9.5659.