PMID- 10026169
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter 
      protein having similar binding and biological properties to Nck.
PG  - 5542-9
AB  - Adapter proteins made up of Src homology (SH) domains mediate multiple cellular
      signaling events initiated by receptor protein tyrosine kinases. Here we report
      that Grb4 is an adapter protein closely related to but distinct from Nck that is 
      made up of three SH3 domains and one SH2 domain. Northern analysis indicated that
      both genes are expressed in multiple tissues. Both Nck and Grb4 proteins could
      associate with receptor tyrosine kinases and the SH3-binding proteins PAK, Sos1, 
      and PRK2, and they synergized with v-Abl and Sos to induce gene expression via
      the transcription factor Elk-1. Although neither protein was transforming on its 
      own, both Nck and Grb4 cooperated with v-Abl to transform NIH 3T3 cells and
      influenced the morphology and anchorage-dependent growth of wild type
      Ras-transformed cells. Nck and Grb4 therefore appear to be functionally
      redundant.
FAU - Braverman, L E
AU  - Braverman LE
AD  - Department of Biochemistry and Molocular Biology and Walther Oncology Center,
      Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
FAU - Quilliam, L A
AU  - Quilliam LA
LA  - eng
GR  - CA63139/CA/NCI NIH HHS/United States
GR  - P60 DK20542-16/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (NCK2 protein, human)
RN  - 0 (Nck protein)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Oncogene Proteins v-abl)
RN  - 0 (RNA, Messenger)
RN  - 0 (Son of Sevenless Proteins)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - DNA, Complementary
MH  - Humans
MH  - Membrane Proteins/metabolism
MH  - Molecular Sequence Data
MH  - Oncogene Proteins/chemistry/*metabolism
MH  - Oncogene Proteins v-abl/metabolism
MH  - Protein Binding
MH  - RNA, Messenger/genetics
MH  - Sequence Homology, Amino Acid
MH  - Son of Sevenless Proteins
MH  - Transcriptional Activation
MH  - *src Homology Domains
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5542 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5542-9. doi: 10.1074/jbc.274.9.5542.