PMID- 10026155
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Novel proteoglycan linkage tetrasaccharides of human urinary soluble
      thrombomodulin, SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1-4Xyl.
PG  - 5436-42
AB  - O-linked sugar chains with xylose as a reducing end linked to human urinary
      soluble thrombomodulin were studied. Sugar chains were liberated by
      hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two
      fractions containing pyridylaminated Xyl as a reducing end were collected. Their 
      structures were determined by partial acid hydrolysis, two-dimensional sugar
      mapping combined with exoglycosidase digestions, methylation analysis, mass
      spectrometry, and NMR as SO4-3GlcAbeta1-3Galbeta1-3(+/-Siaalpha2-6)Galbeta1+
      ++-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is
      believed to be the first example of a proteoglycan linkage tetrasaccharide with
      glucuronic acid 3-sulfate and sialic acid.
FAU - Wakabayashi, H
AU  - Wakabayashi H
AD  - Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, 
      Osaka 560-0043, Japan.
FAU - Natsuka, S
AU  - Natsuka S
FAU - Mega, T
AU  - Mega T
FAU - Otsuki, N
AU  - Otsuki N
FAU - Isaji, M
AU  - Isaji M
FAU - Naotsuka, M
AU  - Naotsuka M
FAU - Koyama, S
AU  - Koyama S
FAU - Kanamori, T
AU  - Kanamori T
FAU - Sakai, K
AU  - Sakai K
FAU - Hase, S
AU  - Hase S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Oligosaccharides)
RN  - 0 (Proteoglycans)
RN  - 0 (Thrombomodulin)
SB  - IM
MH  - Binding Sites, Antibody
MH  - Carbohydrate Conformation
MH  - Carbohydrate Sequence
MH  - Chromatography, Gel
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Hydrolysis
MH  - Molecular Sequence Data
MH  - Oligosaccharides/*chemistry/immunology/isolation & purification
MH  - Proteoglycans/*chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Thrombomodulin/*chemistry
MH  - Urine/chemistry
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5436 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5436-42. doi: 10.1074/jbc.274.9.5436.