PMID- 10026148
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 9
DP  - 1999 Feb 26
TI  - Early embryonic death of mice deficient in gamma-adaptin.
PG  - 5385-90
AB  - Intracellular protein transport and sorting by vesicles in the secretory and
      endocytic pathways requires the formation of a protein coat on the membrane. The 
      heterotetrameric adaptor protein complex 1 (AP-1) promotes the formation of
      clathrin-coated vesicles at the trans-Golgi network. AP-1 interacts with various 
      sorting signals in the cytoplasmic tails of cargo molecules, thus indicating a
      function in protein sorting. We generated mutants of the gamma-adaptin subunit of
      AP-1 in mice to investigate its role in post-Golgi vesicle transport and sorting 
      processes. gamma-Adaptin-deficient embryos develop until day 3.5 post coitus and 
      die during the prenidation period, revealing that AP-1 is essential for
      viability. In heterozygous mice the amount of AP-1 complexes is reduced to half
      of controls. Free beta1- or micro1 chains were not detectable, indicating that
      they are unstable unless they are part of AP-1 complexes. Heterozygous mice weigh
      less then their wild-type littermates and show impaired T cell development.
FAU - Zizioli, D
AU  - Zizioli D
AD  - Zentrum fur Biochemie und Molekulare Zellbiologie, Dept. Biochemie II,
      Universitat Gottingen, Gosslerstrasse 12D, 37073 Gottingen, Germany.
FAU - Meyer, C
AU  - Meyer C
FAU - Guhde, G
AU  - Guhde G
FAU - Saftig, P
AU  - Saftig P
FAU - von Figura, K
AU  - von Figura K
FAU - Schu, P
AU  - Schu P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Protein Complex gamma Subunits)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Adaptor Protein Complex gamma Subunits
MH  - Animals
MH  - Fetal Death/*genetics
MH  - *Genes, Lethal
MH  - Heterozygote
MH  - Membrane Proteins/*genetics
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Phenotype
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1074/jbc.274.9.5385 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Feb 26;274(9):5385-90. doi: 10.1074/jbc.274.9.5385.