PMID- 10025949
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 443
IP  - 3
DP  - 1999 Jan 29
TI  - AMP-activated protein kinase phosphorylation of endothelial NO synthase.
PG  - 285-9
AB  - The AMP-activated protein kinase (AMPK) in rat skeletal and cardiac muscle is
      activated by vigorous exercise and ischaemic stress. Under these conditions AMPK 
      phosphorylates and inhibits acetyl-coenzyme A carboxylase causing increased
      oxidation of fatty acids. Here we show that AMPK co-immunoprecipitates with
      cardiac endothelial NO synthase (eNOS) and phosphorylates Ser-1177 in the
      presence of Ca2+-calmodulin (CaM) to activate eNOS both in vitro and during
      ischaemia in rat hearts. In the absence of Ca2+-calmodulin, AMPK also
      phosphorylates eNOS at Thr-495 in the CaM-binding sequence, resulting in
      inhibition of eNOS activity but Thr-495 phosphorylation is unchanged during
      ischaemia. Phosphorylation of eNOS by the AMPK in endothelial cells and myocytes 
      provides a further regulatory link between metabolic stress and cardiovascular
      function.
FAU - Chen, Z P
AU  - Chen ZP
AD  - St. Vincent's Institute of Medical Research, St. Vincent's Hospital, Fitzroy,
      Vic., Australia.
FAU - Mitchelhill, K I
AU  - Mitchelhill KI
FAU - Michell, B J
AU  - Michell BJ
FAU - Stapleton, D
AU  - Stapleton D
FAU - Rodriguez-Crespo, I
AU  - Rodriguez-Crespo I
FAU - Witters, L A
AU  - Witters LA
FAU - Power, D A
AU  - Power DA
FAU - Ortiz de Montellano, P R
AU  - Ortiz de Montellano PR
FAU - Kemp, B E
AU  - Kemp BE
LA  - eng
GR  - DK35712/DK/NIDDK NIH HHS/United States
GR  - GM25515/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Calmodulin)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Recombinant Proteins)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Aorta
MH  - Calmodulin/metabolism
MH  - Cattle
MH  - Endothelium, Vascular/cytology/*enzymology
MH  - Enzyme Activation
MH  - Kinetics
MH  - Liver/enzymology
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/*metabolism
MH  - Myocardial Ischemia/enzymology
MH  - Myocardium/enzymology
MH  - Nitric Oxide Synthase/*metabolism
MH  - Nitric Oxide Synthase Type III
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Kinases/*metabolism
MH  - *Protein-Serine-Threonine Kinases
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Serine/metabolism
MH  - Threonine/metabolism
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - S0014-5793(98)01705-0 [pii]
AID - 10.1016/s0014-5793(98)01705-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Jan 29;443(3):285-9. doi: 10.1016/s0014-5793(98)01705-0.