PMID- 10025673
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20161124
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 72
IP  - 1
DP  - 1999 Jan 1
TI  - CSF-1 stimulated multiubiquitination of the CSF-1 receptor and of Cbl follows 
      their tyrosine phosphorylation and association with other signaling proteins.
PG  - 119-34
AB  - Addition of colony stimulating factor-1 (CSF-1) to macrophages stimulates the 
      rapid, transient tyrosine phosphorylation, membrane association and 
      multiubiquitination of Cbl (Wang et al. [1996] J. Biol. Chem. 271:17-20). Kinetic 
      analysis reveals that the tyrosine phosphorylation of Cbl is coincident with its 
      plasma membrane translocation and association with the activated tyrosine 
      phosphorylated CSF-1 R, p85, Grb2, and tyrosine phosphorylated p58Shc and that 
      these events precede the simultaneous multiubiquitination of Cbl and the CSF-1 R. 
      Tyrosine phosphorylation and multiubiquitination of the cell surface CSF-1 R are 
      stoichiometric and the multiubiquitinated CSF-1 R is degraded. Similarly, the 
      membrane associated Cbl is almost stoichiometrically ubiquitinated, but the 
      ubiquitinated Cbl is not degraded, being recovered, deubiquitinated, in the 
      cytosol 3-10 min after stimulation at 37 degrees C. In the membrane fraction of 
      cells stimulated at 4 degrees C, the association of p58Shc and Grb2 with Cbl is 
      stable, whereas its association with Sos and p85 is transient and their 
      dissociation occurs at the time CSF-1 R and Cbl multiubiquitination commence. The 
      membrane translocation and the pattern of association of Sos with the CSF-1R, 
      p85, Grb2, and p58Shc resemble those of Cbl but Sos is not tyrosine 
      phosphorylated, nor multiubiquitinated and the coprecipitation of these proteins, 
      other than Grb2, with Sos is much less. Complexes formed by Sos and Cbl are 
      largely independent and membrane complexes of Cbl with other tyrosine 
      phosphorylated proteins, p85 and Grb2 also contain CSF-1 R. These data raise the 
      possibility that the predicted negative regulatory role of Cbl in macrophages is 
      its enhancement of ligand-induced CSF-1 R internalization/degradation.
FAU - Wang, Y
AU  - Wang Y
AD  - Department of Developmental and Molecular Biology, Albert Einstein College of 
      Medicine, Bronx, New York 10461, USA.
FAU - Yeung, Y G
AU  - Yeung YG
FAU - Stanley, E R
AU  - Stanley ER
LA  - eng
GR  - CA 26504/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (Grb2 protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Ubiquitins)
RN  - 42HK56048U (Tyrosine)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-cbl)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 6.3.2.- (Cbl protein, mouse)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Fluorescent Antibody Technique
MH  - GRB2 Adaptor Protein
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Proteins/metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-cbl
MH  - Receptor, Macrophage Colony-Stimulating Factor/drug effects/*metabolism
MH  - Signal Transduction/physiology
MH  - Tyrosine/*metabolism
MH  - *Ubiquitin-Protein Ligases
MH  - Ubiquitins/*metabolism
EDAT- 1999/02/20 03:12
MHDA- 2000/06/20 09:00
CRDT- 1999/02/20 03:12
PHST- 1999/02/20 03:12 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/20 03:12 [entrez]
AID - 10.1002/(SICI)1097-4644(19990101)72:1<119::AID-JCB13>3.0.CO;2-R [pii]
PST - ppublish
SO  - J Cell Biochem. 1999 Jan 1;72(1):119-34.