PMID- 10025505
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20131121
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 18
IP  - 1
DP  - 1999 Jan
TI  - cDNA, genomic cloning, and gene expression analysis of mouse PSP94 (prostate
      secretory protein of 94 amino acids).
PG  - 11-26
AB  - The potential use of prostate secretory protein of 94 amino acids (PSP94) as a
      diagnostic biomarker or a therapeutic agent for prostate cancer has been
      reported. In order to establish an animal model to further elucidate on its
      biological role, we cloned the mouse PSP94 cDNA (approximately 500 bp) by reverse
      transcriptase-polymerase chain reaction (RT-PCR) and disclosed its genomic
      structure. The whole mouse PSP94 gene (approximately 23 kb) was amplified by long
      and accurate-PCR and also cloned by screening of a mouse embryo stem-cell genomic
      library. Computational and statistical analyses have demonstrated several highly 
      conserved characteristics of PSP94 among different species. Comparison of PSP94
      from human, two primates, pig, and rodents revealed that the most significant
      feature is that PSP94 is rich in cysteines (10% of the total sequence) and their 
      positions are highly conserved. The three intron-four exon structure of the human
      PSP94 gene and the consensus sequence (....GT-intron-AG...) for mRNA splicing are
      also strongly conserved. A high divergence in cDNA sequence in the protein-coding
      region and also in the genomic sequence of PSP94 was also observed among these
      species. Comparing with alpha-globin, a typical evolutionally conserved gene,
      with the PSP94 gene, the rate of nonsynonymous changes per site per year (kN) is 
      2 to 6 times higher, indicating that PSP94 gene has been under far fewer
      evolutionary constraints than other genes and has a potential role as a species
      barrier in reproductive biology. In order to test this hypothesis, we
      investigated the gene expression of PSP94 and its tissue distribution in various 
      rodent tissues by RT-PCR and in situ hybridization (ISH). Gene expression was
      found only in the prostate, suggesting that PSP94 is probably more tissue
      specific in the prostate of rodents than in mammals. The ISH analysis also
      revealed a prostate lobe-specific expression of the PSP94 gene in both mice and
      rats. It was strongly expressed in the lateral prostate, but the findings were
      negative in the dorsal and ventral lobe. Therefore, it is hypothesized that one
      of the primary functions of rodent PSP94, as a major prostate secretory protein, 
      is related to reproductive biology.
FAU - Xuan, J W
AU  - Xuan JW
AD  - Department of Surgery, University of Western Ontario, London, Canada.
FAU - Kwong, J
AU  - Kwong J
FAU - Chan, F L
AU  - Chan FL
FAU - Ricci, M
AU  - Ricci M
FAU - Imasato, Y
AU  - Imasato Y
FAU - Sakai, H
AU  - Sakai H
FAU - Fong, G H
AU  - Fong GH
FAU - Panchal, C
AU  - Panchal C
FAU - Chin, J L
AU  - Chin JL
LA  - eng
SI  - GENBANK/AF033264
SI  - GENBANK/AF033265
SI  - GENBANK/AF033266
SI  - GENBANK/AF039596
SI  - GENBANK/AF136292
SI  - GENBANK/AH007413
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (DNA, Complementary)
RN  - 0 (Peptides)
RN  - 0 (Prostatic Secretory Proteins)
RN  - 0 (Proteins)
RN  - 0 (Seminal Plasma Proteins)
RN  - 0 (beta-microseminoprotein)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - *Cloning, Molecular
MH  - Cysteine/genetics
MH  - DNA, Complementary/*genetics
MH  - Evolution, Molecular
MH  - *Gene Expression
MH  - *Genome
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Peptides/chemistry/*genetics
MH  - Prostate/*metabolism
MH  - *Prostatic Secretory Proteins
MH  - Proteins/chemistry/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminal Plasma Proteins
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1089/104454999315583 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1999 Jan;18(1):11-26. doi: 10.1089/104454999315583.