PMID- 10025403
OWN - NLM
STAT- MEDLINE
DCOM- 19990303
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 3
DP  - 1999 Feb 5
TI  - Crystal structures of two Sm protein complexes and their implications for the
      assembly of the spliceosomal snRNPs.
PG  - 375-87
AB  - The U1, U2, U4/U6, and U5 small nuclear ribonucleoprotein particles (snRNPs)
      involved in pre-mRNA splicing contain seven Sm proteins (B/B', D1, D2, D3, E, F, 
      and G) in common, which assemble around the Sm site present in four of the major 
      spliceosomal small nuclear RNAs (snRNAs). These proteins share a common sequence 
      motif in two segments, Sm1 and Sm2, separated by a short variable linker. Crystal
      structures of two Sm protein complexes, D3B and D1D2, show that these proteins
      have a common fold containing an N-terminal helix followed by a strongly bent
      five-stranded antiparallel beta sheet, and the D1D2 and D3B dimers superpose
      closely in their core regions, including the dimer interfaces. The crystal
      structures suggest that the seven Sm proteins could form a closed ring and the
      snRNAs may be bound in the positively charged central hole.
FAU - Kambach, C
AU  - Kambach C
AD  - MRC Laboratory of Molecular Biology, Cambridge, England, UK.
FAU - Walke, S
AU  - Walke S
FAU - Young, R
AU  - Young R
FAU - Avis, J M
AU  - Avis JM
FAU - de la Fortelle, E
AU  - de la Fortelle E
FAU - Raker, V A
AU  - Raker VA
FAU - Luhrmann, R
AU  - Luhrmann R
FAU - Li, J
AU  - Li J
FAU - Nagai, K
AU  - Nagai K
LA  - eng
SI  - PDB/1B34
SI  - PDB/1D3B
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Autoantigens)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (Sm protein D2)
RN  - 0 (Sm protein D3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Autoantigens/chemistry/metabolism
MH  - Conserved Sequence
MH  - Crystallography, X-Ray
MH  - Dimerization
MH  - Macromolecular Substances
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry/metabolism
MH  - Protein Folding
MH  - Protein Structure, Tertiary
MH  - RNA Splicing
MH  - RNA, Small Nuclear/metabolism
MH  - Ribonucleoproteins, Small Nuclear/*chemistry/metabolism
MH  - Spliceosomes/*chemistry/metabolism
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - S0092-8674(00)80550-4 [pii]
AID - 10.1016/s0092-8674(00)80550-4 [doi]
PST - ppublish
SO  - Cell. 1999 Feb 5;96(3):375-87. doi: 10.1016/s0092-8674(00)80550-4.