PMID- 10024882
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20131121
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 1
DP  - 1999 Jan
TI  - Reconstitution of the transcription factor TFIIH: assignment of functions for the
      three enzymatic subunits, XPB, XPD, and cdk7.
PG  - 87-95
AB  - To understand the initiation of the transcription of protein-coding genes, we
      have dissected the role of the basal transcription/DNA repair factor TFIIH.
      Having succeeded in reconstituting a functionally active TFIIH from baculovirus
      recombinant polypeptides, we were able to analyze the role of XPB, XPD, and cdk7 
      subunits in the transcription reaction. Designing mutated recombinant subunits,
      we show that the XPB helicase is absolutely required for transcription to open
      the promoter around the start site whereas the XPD helicase, which is
      dispensable, stimulates transcription and allows the CAK complex to be anchored
      to TFIIH. In addition, we also show that cdk7 may phosphorylate the
      carboxy-terminal domain (CTD) of RNA pol II in the absence of promoter opening.
FAU - Tirode, F
AU  - Tirode F
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM/ULP, 
      Illkirch, C. U. de Strasbourg, France.
FAU - Busso, D
AU  - Busso D
FAU - Coin, F
AU  - Coin F
FAU - Egly, J M
AU  - Egly JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
RN  - 146045-44-5 (XPBC-ERCC-3 protein)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.11.22 (cyclin-dependent kinase-activating kinase)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (Xeroderma Pigmentosum Group D Protein)
RN  - EC 5.99.- (ERCC2 protein, human)
SB  - IM
MH  - Baculoviridae/genetics
MH  - *Cyclin-Dependent Kinases
MH  - DNA Helicases/genetics/metabolism
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Mutation/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Protein-Serine-Threonine Kinases/genetics/metabolism
MH  - Proteins/genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - *Transcription Factors
MH  - Transcription Factors, TFII/*genetics/metabolism
MH  - Transcription, Genetic/*genetics
MH  - Xeroderma Pigmentosum Group D Protein
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - S1097-2765(00)80177-X [pii]
PST - ppublish
SO  - Mol Cell. 1999 Jan;3(1):87-95.