PMID- 10024668
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 3
DP  - 1999 Mar
TI  - Tissue specific expression and chromosomal mapping of a human
      UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1,
      4-N-acetylglucosaminyltransferase.
PG  - 303-10
AB  - A human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N-
      acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library
      using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA
      encoded a complete sequence of a type II membrane protein of 535 amino acids
      which is 96% identical to the bovine GnT-IV. Transient expression of the human
      cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold,
      demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot
      analysis of normal tissues indicated that at least five different sizes of mRNA
      (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore,
      these mRNAs are expressed at different levels between tissues. Large amounts of
      mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic 
      leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed
      abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2,
      band q12 by fluorescent in situ hybridization.
FAU - Yoshida, A
AU  - Yoshida A
AD  - Glycotechnology Group, Central Laboratories for Key Technology, Kirin Brewery
      Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
FAU - Minowa, M T
AU  - Minowa MT
FAU - Takamatsu, S
AU  - Takamatsu S
FAU - Hara, T
AU  - Hara T
FAU - Oguri, S
AU  - Oguri S
FAU - Ikenaga, H
AU  - Ikenaga H
FAU - Takeuchi, M
AU  - Takeuchi M
LA  - eng
SI  - GENBANK/AB000616
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
RN  - EC 2.4.1.145 (alpha-1,3-mannosylglycoprotein
      beta-1,4-N-acetylglucosaminyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - COS Cells
MH  - Cell Lineage
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2/*genetics
MH  - DNA, Complementary/genetics
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/enzymology
MH  - Lymphoid Tissue/enzymology
MH  - Membrane Proteins/*genetics
MH  - Molecular Sequence Data
MH  - N-Acetylglucosaminyltransferases/*genetics
MH  - Neoplasm Proteins/genetics
MH  - RNA, Messenger/isolation & purification
MH  - Recombinant Proteins/biosynthesis
MH  - T-Lymphocytes/*enzymology
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - cwc029 [pii]
AID - 10.1093/glycob/9.3.303 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Mar;9(3):303-10. doi: 10.1093/glycob/9.3.303.