PMID- 10024665
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 3
DP  - 1999 Mar
TI  - Alternative splicing of transcripts encoding the alpha- and beta-subunits of
      mouse glucosidase II in T lymphocytes.
PG  - 277-83
AB  - Glucosidase II is a processing enzyme of the endoplasmic reticulum that functions
      to hydrolyze two glucose residues in immature N -linked oligosaccharides attached
      to newly synthesized polypeptides. We previously reported the cDNA cloning of the
      alpha- and beta-subunits of mouse glucosidase II from T cells following
      copurification of these proteins with the highly glycosylated transmembrane
      protein-tyrosine phosphatase CD45. Subsequent examination of additional cDNA
      clones, coupled with partial genomic DNA sequencing, has revealed that both
      subunits are encoded by gene products that undergo alternative splicing in T
      lymphocytes. The catalytic alpha-subunit possesses two variably expressed
      segments, box Alpha1, consisting of 22 amino acids located proximal to the
      amino-terminus, and box Alpha2, composed of 9 amino acids situated between the
      amino-terminus and the putative catalytic site in the central region of the
      molecule. Box Beta1, a variably expressed 7 amino acid segment in the
      beta-subunit of glucosidase II, is located immediately downstream of an acidic
      stretch near the carboxyl-terminus. Screening of reverse transcribed RNA by
      polymerase chain reaction confirms the variable inclusion of each of these
      segments in transcripts obtained from a panel of T-lymphocyte cell lines. Thus,
      distinct isoforms of glucosidase II exist that may perform specialized functions.
FAU - Arendt, C W
AU  - Arendt CW
AD  - Department of Medical Microbiology and Immunology, University of Alberta,
      Edmonton T6G 2H7, Canada.
FAU - Dawicki, W
AU  - Dawicki W
FAU - Ostergaard, H L
AU  - Ostergaard HL
LA  - eng
SI  - GENBANK/AF066057
SI  - GENBANK/AF066058
SI  - GENBANK/AF066059
SI  - GENBANK/AF066060
SI  - GENBANK/AF066061
SI  - GENBANK/AH007958
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - EC 3.2.1.- (4-nitrophenyl-alpha-glucosidase)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary/genetics
MH  - Genetic Heterogeneity
MH  - Genome
MH  - Isoenzymes/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA Precursors/genetics
MH  - RNA, Messenger/genetics
MH  - Sequence Analysis
MH  - T-Lymphocytes/*enzymology
MH  - alpha-Glucosidases/*genetics
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - cwc026 [pii]
AID - 10.1093/glycob/9.3.277 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Mar;9(3):277-83. doi: 10.1093/glycob/9.3.277.