PMID- 10024660
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20190513
IS  - 0959-6658 (Print)
IS  - 0959-6658 (Linking)
VI  - 9
IP  - 3
DP  - 1999 Mar
TI  - Structural characterization of the N-linked oligosaccharides in bile
      salt-stimulated lipase originated from human breast milk.
PG  - 227-34
AB  - The detailed structures of N- glycans derived from bile salt-stimulated lipase
      (BSSL) found in human milk were determined by combining exoglycosidase digestion 
      with matrix-assisted laser desorption/ionization time-of-flight mass
      spectrometry. The N- glycan structures were conclusively determined in terms of
      complexity and degree of fucosylation. Ion-exchange chromatography with pulsed
      amperometric detection, together with mass-spectral analysis of the esterified N-
      glycans, indicated the presence of monosialylated structures. The molecular mass 
      profile of esterified N- glycans present in BSSL further permitted the more
      detailed studies through collision-induced dissociation (CID) and sequential
      exoglycosidase cleavages. The N- glycan structures were elucidated to be
      complex/dibranched, fucosylated/complex/dibranched,
      monosialylated/complex/dibranched, and monosialylated/fucosylated/dibranched
      entities.
FAU - Mechref, Y
AU  - Mechref Y
AD  - Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
FAU - Chen, P
AU  - Chen P
FAU - Novotny, M V
AU  - Novotny MV
LA  - eng
GR  - GM24349/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (alpha-Fetoproteins)
RN  - EC 3.1.1.- (bile salt-stimulated lipase)
RN  - EC 3.1.1.13 (Sterol Esterase)
SB  - IM
MH  - Carbohydrate Sequence
MH  - Female
MH  - Glycoproteins/*chemistry
MH  - Humans
MH  - Milk, Human/*enzymology
MH  - Molecular Sequence Data
MH  - Oligosaccharides/*chemistry
MH  - Sequence Analysis
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Sterol Esterase/*chemistry
MH  - alpha-Fetoproteins/chemistry
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - cwc019 [pii]
AID - 10.1093/glycob/9.3.227 [doi]
PST - ppublish
SO  - Glycobiology. 1999 Mar;9(3):227-34. doi: 10.1093/glycob/9.3.227.