PMID- 10023815
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20101118
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 405
IP  - 2
DP  - 1999 Mar 8
TI  - Tectorin mRNA expression is spatially and temporally restricted during mouse
      inner ear development.
PG  - 271-80
AB  - The tectorial and otolithic membranes are extracellular matrices that cover the
      sensory epithelia of the inner ear. They are required for mechanotransduction and
      may influence hair-cell development. The mRNA expression patterns for two major
      glycoproteins of these matrices, alpha- and beta-tectorin, were examined during
      mouse inner ear development to determine when and where these proteins are
      produced relative to hair cells and whether tectorin production is continuous or 
      transient. Using in situ hybridisation, alpha- and beta-tectorin mRNAs are first 
      detected in the basal end of the cochlea at embryonic day (E) 12.5, and the
      distinct patterns observed for each tectorin mRNA in the neonate become visible
      by E14.5. The neonatal expression patterns indicate that some cell types in the
      cochlea express both alpha- and beta-tectorin mRNAs, while other cells only
      express one tectorin mRNA. Although expressed early in development, alpha- and
      beta-tectorin mRNAs cannot be detected in the cochlea by postnatal day (P) 22. In
      the saccule and utricle, alpha-tectorin mRNA is detected at E12.5, but
      beta-tectorin mRNA is not observed until E14.5. Expression of alpha-tectorin mRNA
      ceases after P15, whereas beta-tectorin mRNA expression continues within the
      striolar region of the utricle until at least P150. The results show alpha- and
      beta-tectorin mRNAs are expressed during the early stages of inner ear
      development, prior to or concomitant with hair-cell differentiation, and before
      the appearance of hair bundles. The expression patterns suggest different cell
      types contribute to the formation of the various regions of the tectorial
      membrane. Although tectorin mRNAs are only expressed transiently during cochlear 
      development, beta-tectorin mRNA is continuously expressed within the striolar
      region of the utricle.
FAU - Rau, A
AU  - Rau A
AD  - School of Biological Sciences, University of Sussex, Falmer, Brighton, United
      Kingdom.
FAU - Legan, P K
AU  - Legan PK
FAU - Richardson, G P
AU  - Richardson GP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (TECTB protein, human)
RN  - 0 (Tecta protein, mouse)
SB  - IM
MH  - Animals
MH  - Cochlea/embryology/growth & development/metabolism
MH  - Ear, Inner/embryology/growth & development/*metabolism
MH  - Embryonic and Fetal Development/physiology
MH  - Extracellular Matrix Proteins/*genetics
MH  - GPI-Linked Proteins
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - In Situ Hybridization
MH  - Membrane Glycoproteins/*genetics
MH  - Membrane Proteins/*genetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - RNA, Messenger/*biosynthesis
MH  - Time Factors
MH  - Vestibule, Labyrinth/embryology/growth & development/metabolism
EDAT- 1999/02/19 04:31
MHDA- 2000/06/20 09:00
CRDT- 1999/02/19 04:31
PHST- 1999/02/19 04:31 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/19 04:31 [entrez]
AID - 10.1002/(SICI)1096-9861(19990308)405:2<271::AID-CNE10>3.0.CO;2-2 [pii]
PST - ppublish
SO  - J Comp Neurol. 1999 Mar 8;405(2):271-80.