PMID- 10023782
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20191102
IS  - 0946-2716 (Print)
IS  - 0946-2716 (Linking)
VI  - 77
IP  - 2
DP  - 1999 Feb
TI  - A novel isoform of the smooth muscle cell differentiation marker smoothelin.
PG  - 294-8
AB  - Studies on smooth muscle cell differentiation and those on vascular development
      in mouse and humans have long been hampered by the lack of suitable markers. Here
      we describe a novel, large isoform of smoothelin, a structural protein of
      differentiated, contractile smooth muscle cells. The protein, which is highly
      conserved in mouse and humans, shows homology with other cytoskeleton-associated 
      smooth muscle cell proteins and contains an actinin-type actin-binding domain.
      Northern blot analysis from various mouse organs identified short and long
      smoothelin mRNA forms, which exhibit distinct tissue expression patterns. The
      short form is highly expressed in visceral muscle tissues such as intestine and
      stomach and is not detectable in brain, while the long mRNA form is expressed in 
      all vascularized organs. These results may provide new tools and approaches to
      study both smooth muscle cell differentiation and proliferative vascular disease.
FAU - Kramer, J
AU  - Kramer J
AD  - Max Delbruck Center for Molecular Medicine, Berlin, Germany.
FAU - Aguirre-Arteta, A M
AU  - Aguirre-Arteta AM
FAU - Thiel, C
AU  - Thiel C
FAU - Gross, C M
AU  - Gross CM
FAU - Dietz, R
AU  - Dietz R
FAU - Cardoso, M C
AU  - Cardoso MC
FAU - Leonhardt, H
AU  - Leonhardt H
LA  - eng
SI  - GENBANK/AJ010305
SI  - GENBANK/AJ010306
SI  - GENBANK/Y13492
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Mol Med (Berl)
JT  - Journal of molecular medicine (Berlin, Germany)
JID - 9504370
RN  - 0 (Actins)
RN  - 0 (Biomarkers)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (SMTN protein, human)
RN  - 0 (Smtn protein, mouse)
RN  - 11003-00-2 (Actinin)
SB  - IM
CIN - J Mol Med (Berl). 1999 Feb;77(2):255-7. PMID: 10023778
EIN - J Mol Med 1999 Apr;77(4):399
MH  - Actinin/metabolism
MH  - Actins/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Biomarkers
MH  - Cell Differentiation/*physiology
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Cytoskeletal Proteins/chemistry/*genetics/*metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Muscle Proteins/chemistry/*genetics/*metabolism
MH  - Muscle, Smooth/*cytology/*metabolism
MH  - Organ Specificity
MH  - Protein Isoforms
MH  - Sequence Homology, Amino Acid
EDAT- 1999/02/19 00:00
MHDA- 1999/02/19 00:01
CRDT- 1999/02/19 00:00
PHST- 1999/02/19 00:00 [pubmed]
PHST- 1999/02/19 00:01 [medline]
PHST- 1999/02/19 00:00 [entrez]
AID - 10.1007/s001090050352 [doi]
PST - ppublish
SO  - J Mol Med (Berl). 1999 Feb;77(2):294-8. doi: 10.1007/s001090050352.