PMID- 10023675
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20171116
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 4
DP  - 1999 Jan 28
TI  - Physical interaction of the bHLH LYL1 protein and NF-kappaB1 p105.
PG  - 995-1005
AB  - The LYL1 gene was first identified upon the molecular characterization of the
      t(7;9)(q35;p13) translocation associated with some human T-cell acute leukemias
      (T-ALLs). In adult tissues, LYL1 expression is restricted to hematopoietic cells 
      with the notable exclusion of the T cell lineage. LYL1 encodes a basic
      helix-loop-helix (bHLH) protein highly related to TAL-1, whose activation is also
      associated with a high proportion of human T-ALLs. A yeast two-hybrid system was 
      used to identify proteins that specifically interact with LYL1 and might mediate 
      its activities. We found that p105, the precursor of NF-kappaB1 p50, was the
      major LYL1-interacting protein in this system. The association between LYL1 and
      p105 was confirmed both in vitro and in vivo in mammalian cells. Biochemical
      studies indicated that the interaction was mediated by the bHLH motif of LYL1 and
      the ankyrin-like motifs of p105. Ectopic expression of LYL1 in a human T cell
      line caused a significant decrease in NF-kappaB-dependent transcription,
      associated with a reduced level of NF-kappaB1 proteins.
FAU - Ferrier, R
AU  - Ferrier R
AD  - Institut de Genetique Moleculaire, UMR 5535, Montpellier, France.
FAU - Nougarede, R
AU  - Nougarede R
FAU - Doucet, S
AU  - Doucet S
FAU - Kahn-Perles, B
AU  - Kahn-Perles B
FAU - Imbert, J
AU  - Imbert J
FAU - Mathieu-Mahul, D
AU  - Mathieu-Mahul D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (LYL1 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (T-Cell Acute Lymphocytic Leukemia Protein 1)
RN  - 0 (Transcription Factors)
RN  - 135471-20-4 (TAL1 protein, human)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cell Line
MH  - DNA, Complementary/genetics
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Glutathione Transferase/metabolism
MH  - *Helix-Loop-Helix Motifs
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Jurkat Cells
MH  - K562 Cells
MH  - Leukemia-Lymphoma, Adult T-Cell/metabolism
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/genetics/*metabolism
MH  - NF-kappa B p50 Subunit
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Protein Precursors/genetics/*metabolism
MH  - *Proto-Oncogene Proteins
MH  - T-Cell Acute Lymphocytic Leukemia Protein 1
MH  - *Transcription Factors
EDAT- 1999/02/19 00:00
MHDA- 1999/02/19 00:01
CRDT- 1999/02/19 00:00
PHST- 1999/02/19 00:00 [pubmed]
PHST- 1999/02/19 00:01 [medline]
PHST- 1999/02/19 00:00 [entrez]
AID - 10.1038/sj.onc.1202374 [doi]
PST - ppublish
SO  - Oncogene. 1999 Jan 28;18(4):995-1005. doi: 10.1038/sj.onc.1202374.