PMID- 10023073
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20191210
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 227
IP  - 2
DP  - 1999 Feb 18
TI  - The human genome has only one functional hsp47 gene (CBP2) and a pseudogene
      (pshsp47).
PG  - 241-8
AB  - Among all the species investigated to date, only in humans is hsp47 reported to
      exist as two separate genes. Here we examined whether hsp47 forms a gene family, 
      and if so, how many genes constitute the family. Cloning and sequencing of human 
      hsp47 cDNA revealed that only one gene, identical to CBP2, was transcribed. No
      transcript corresponding to colligin, which was reported to be a human homologue 
      of hsp47, was found. Genomic southern hybridization using the exon III fragment
      of mouse hsp47 as a probe, however, showed two bands for several restriction
      enzyme digests. We cloned and sequenced the gene corresponding to the extra band 
      and found that a pseudogene (pshsp47) existed in the human genome. We have mapped
      this pseudogene to chromosome 9p12-p13 by fluorescent in situ hybridization
      (FISH) using a 3.5kb genomic fragment containing the entire pshsp47 sequence as a
      probe. These results suggested that functional hsp47 exists as CBP2, not as
      colligin, and a highly conserved pseudogene is present in the human genome.
FAU - Nagai, N
AU  - Nagai N
AD  - Department of Molecular and Cellular Biology, Institute for Frontier Medical
      Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8397, CREST, JST, Japan.
FAU - Tetuya, Y
AU  - Tetuya Y
FAU - Hosokawa, N
AU  - Hosokawa N
FAU - Nagata, K
AU  - Nagata K
LA  - eng
SI  - GENBANK/AB010273
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Carrier Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (HSP47 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (NOL6 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SERPINH1 protein, human)
RN  - 0 (SERPINH1P1 protein, human)
RN  - 0 (colligin)
SB  - IM
MH  - Base Sequence
MH  - Carrier Proteins/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 19/genetics
MH  - Cloning, Molecular
MH  - *Gene Dosage
MH  - *Genome, Human
MH  - Glycoproteins
MH  - HSP47 Heat-Shock Proteins
MH  - Heat-Shock Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Nuclear Proteins
MH  - Pseudogenes/*genetics
MH  - RNA, Messenger/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 1999/02/19 00:00
MHDA- 1999/02/19 00:01
CRDT- 1999/02/19 00:00
PHST- 1999/02/19 00:00 [pubmed]
PHST- 1999/02/19 00:01 [medline]
PHST- 1999/02/19 00:00 [entrez]
AID - S0378-1119(98)00592-7 [pii]
AID - 10.1016/s0378-1119(98)00592-7 [doi]
PST - ppublish
SO  - Gene. 1999 Feb 18;227(2):241-8. doi: 10.1016/s0378-1119(98)00592-7.