PMID- 10022980
OWN - NLM
STAT- MEDLINE
DCOM- 19990614
LR  - 20181113
IS  - 1088-9051 (Print)
IS  - 1088-9051 (Linking)
VI  - 9
IP  - 2
DP  - 1999 Feb
TI  - The mouse Aire gene: comparative genomic sequencing, gene organization, and
      expression.
PG  - 158-66
AB  - Mutations in the human AIRE gene (hAIRE) result in the development of an
      autoimmune disease named APECED (autoimmune polyendocrinopathy candidiasis
      ectodermal dystrophy; OMIM 240300). Previously, we have cloned hAIRE and shown
      that it codes for a putative transcription-associated factor. Here we report the 
      cloning and characterization of Aire, the murine ortholog of hAIRE. Comparative
      genomic sequencing revealed that the structure of the AIRE gene is highly
      conserved between human and mouse. The conceptual proteins share 73% homology and
      feature the same typical functional domains in both species. RT-PCR analysis
      detected three splice variant isoforms in various mouse tissues, and
      interestingly one isoform was conserved in human, suggesting potential biological
      relevance of this product. In situ hybridization on mouse and human histological 
      sections showed that AIRE expression pattern was mainly restricted to a few cells
      in the thymus, calling for a tissue-specific function of the gene product.
FAU - Blechschmidt, K
AU  - Blechschmidt K
AD  - Institute of Molecular Biotechnology, Department of Genome Analysis, D-07745
      Jena, Germany.
FAU - Schweiger, M
AU  - Schweiger M
FAU - Wertz, K
AU  - Wertz K
FAU - Poulson, R
AU  - Poulson R
FAU - Christensen, H M
AU  - Christensen HM
FAU - Rosenthal, A
AU  - Rosenthal A
FAU - Lehrach, H
AU  - Lehrach H
FAU - Yaspo, M L
AU  - Yaspo ML
LA  - eng
SI  - GENBANK/AF073797
SI  - GENBANK/AJ132243
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genome Res
JT  - Genome research
JID - 9518021
RN  - 0 (APECED protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - DNA-Binding Proteins/genetics
MH  - Gene Expression/*genetics
MH  - Humans
MH  - In Situ Hybridization
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Polyendocrinopathies, Autoimmune/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA/*methods
MH  - Sequence Homology, Nucleic Acid
MH  - Thymus Gland
MH  - Transcription Factors/*genetics
MH  - Zinc Fingers/genetics
PMC - PMC310712
EDAT- 1999/02/19 00:00
MHDA- 1999/02/19 00:01
CRDT- 1999/02/19 00:00
PHST- 1999/02/19 00:00 [pubmed]
PHST- 1999/02/19 00:01 [medline]
PHST- 1999/02/19 00:00 [entrez]
PST - ppublish
SO  - Genome Res. 1999 Feb;9(2):158-66.