PMID- 10022913
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 3
DP  - 1999 Mar
TI  - Identification and characterization of the human orthologue of yeast Pex14p.
PG  - 2265-77
AB  - Pex14p is a central component of the peroxisomal protein import machinery, which 
      has been suggested to provide the point of convergence for PTS1- and
      PTS2-dependent protein import in yeast cells. Here we describe the identification
      of a human peroxisome-associated protein (HsPex14p) which shows significant
      similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal
      membrane protein with its C terminus exposed to the cytosol. The N terminus of
      the protein is not accessible to exogenously added antibodies or protease and
      thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to 
      the decoration of tubular structures and mislocalization of peroxisomal catalase 
      to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal
      targeting signal 1 (PTS1), a result consistent with a function as a membrane
      receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or
      interaction of the protein with the PTS2-receptor or HsPex13p was not observed.
      This distinguishes the human Pex14p from its counterpart in yeast cells and thus 
      supports recent data suggesting that not all aspects of peroxisomal protein
      import are conserved between yeasts and humans. The role of HsPex14p in mammalian
      peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible
      for an unrecognized complementation group of human peroxisome biogenesis
      disorders.
FAU - Will, G K
AU  - Will GK
AD  - Institut fur Physiologische Chemie, Ruhr-Universitat Bochum, 44780 Bochum,
      Germany.
FAU - Soukupova, M
AU  - Soukupova M
FAU - Hong, X
AU  - Hong X
FAU - Erdmann, K S
AU  - Erdmann KS
FAU - Kiel, J A
AU  - Kiel JA
FAU - Dodt, G
AU  - Dodt G
FAU - Kunau, W H
AU  - Kunau WH
FAU - Erdmann, R
AU  - Erdmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (PEX14 protein, S cerevisiae)
RN  - 0 (PEX14 protein, human)
RN  - 0 (Peroxins)
RN  - 0 (Peroxisome-Targeting Signal 1 Receptor)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Repressor Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - *Carrier Proteins
MH  - DNA, Complementary
MH  - Fungal Proteins/*chemistry
MH  - Gene Expression
MH  - Humans
MH  - Membrane Proteins/*chemistry
MH  - Membrane Transport Proteins
MH  - Microbodies/metabolism
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Peroxins
MH  - Peroxisome-Targeting Signal 1 Receptor
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - *Repressor Proteins
MH  - Saccharomyces cerevisiae
MH  - Saccharomyces cerevisiae Proteins
PMC - PMC84019
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1128/mcb.19.3.2265 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Mar;19(3):2265-77. doi: 10.1128/mcb.19.3.2265.