PMID- 10022909
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 3
DP  - 1999 Mar
TI  - Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which
      localizes to the rough endoplasmic reticulum.
PG  - 2220-30
AB  - Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA 
      transport and localization in Drosophila. To understand the molecular mechanisms 
      of mRNA transport in mammals, we cloned human (hStau) and mouse (mStau) staufen
      cDNAs. In humans, four transcripts arise by differential splicing of the Stau
      gene and code for two proteins with different N-terminal extremities. In vitro,
      hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and 
      tubulin via a region similar to the microtubule-binding domain of MAP-1B,
      suggesting that Stau cross-links cytoskeletal and RNA components.
      Immunofluorescent double labeling of transfected mammalian cells revealed that
      Stau is localized to the rough endoplasmic reticulum (RER), implicating this
      RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process
      involving microtubules. These results are the first demonstration of the
      association of an RNA-binding protein in addition to ribosomal proteins, with the
      RER, implicating this class of proteins in the transport of RNA to its site of
      translation.
FAU - Wickham, L
AU  - Wickham L
AD  - Departments of Biochemistry, University of Montreal, Montreal, Quebec, Canada H3C
      3J7.
FAU - Duchaine, T
AU  - Duchaine T
FAU - Luo, M
AU  - Luo M
FAU - Nabi, I R
AU  - Nabi IR
FAU - DesGroseillers, L
AU  - DesGroseillers L
LA  - eng
SI  - GENBANK/AF061938
SI  - GENBANK/AF061939
SI  - GENBANK/AF061940
SI  - GENBANK/AF061941
SI  - GENBANK/AF061942
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA, Complementary)
RN  - 0 (Detergents)
RN  - 0 (Drosophila Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Tubulin)
RN  - 139568-71-1 (stau protein, Drosophila)
RN  - 9002-93-1 (Octoxynol)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Detergents
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster
MH  - Endoplasmic Reticulum, Rough/*metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Octoxynol
MH  - RNA, Double-Stranded/genetics/*metabolism
MH  - RNA-Binding Proteins
MH  - Sequence Homology, Amino Acid
MH  - Tubulin/*metabolism
PMC - PMC84015
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1128/mcb.19.3.2220 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Mar;19(3):2220-30. doi: 10.1128/mcb.19.3.2220.