PMID- 10022908
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 3
DP  - 1999 Mar
TI  - A human sequence homologue of Staufen is an RNA-binding protein that is
      associated with polysomes and localizes to the rough endoplasmic reticulum.
PG  - 2212-9
AB  - In the course of a two-hybrid screen with the NS1 protein of influenza virus, a
      human clone capable of coding for a protein with high homology to the Staufen
      protein from Drosophila melanogaster (dmStaufen) was identified. With these
      sequences used as a probe, cDNAs were isolated from a lambda cDNA library. The
      encoded protein (hStaufen-like) contained four double-stranded RNA
      (dsRNA)-binding domains with 55% similarity and 38% identity to those of
      dmStaufen, including identity at all residues involved in RNA binding. A
      recombinant protein containing all dsRNA-binding domains was expressed in
      Escherichia coli as a His-tagged polypeptide. It showed dsRNA binding activity in
      vitro, with an apparent Kd of 10(-9) M. Using a specific antibody, we detected in
      human cells a major form of the hStaufen-like protein with an apparent molecular 
      mass of 60 to 65 kDa. The intracellular localization of hStaufen-like protein was
      investigated by immunofluorescence using a series of markers for the cell
      compartments. Colocalization was observed with the rough endoplasmic reticulum
      but not with endosomes, cytoskeleton, or Golgi apparatus. Furthermore,
      sedimentation analyses indicated that hStaufen-like protein associates with
      polysomes. These results are discussed in relation to the possible functions of
      the protein.
FAU - Marion, R M
AU  - Marion RM
AD  - Centro Nacional de Biotecnologia (CSIC), Cantoblanco, 28049 Madrid, Spain.
FAU - Fortes, P
AU  - Fortes P
FAU - Beloso, A
AU  - Beloso A
FAU - Dotti, C
AU  - Dotti C
FAU - Ortin, J
AU  - Ortin J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (INS1 protein, influenza virus)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (STAU1 protein, human)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (staufen protein, mammalian)
RN  - 139568-71-1 (stau protein, Drosophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Cell Line, Transformed
MH  - Cytoskeletal Proteins
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster
MH  - Endoplasmic Reticulum, Rough/*metabolism
MH  - Gene Expression
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polyribosomes/*metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Viral Nonstructural Proteins/genetics/metabolism
PMC - PMC84014
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1128/mcb.19.3.2212 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Mar;19(3):2212-9. doi: 10.1128/mcb.19.3.2212.