PMID- 10022900
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 3
DP  - 1999 Mar
TI  - The general transcription factors IIA, IIB, IIF, and IIE are required for RNA
      polymerase II transcription from the human U1 small nuclear RNA promoter.
PG  - 2130-41
AB  - RNA polymerase II transcribes the mRNA-encoding genes and the majority of the
      small nuclear RNA (snRNA) genes. The formation of a minimal functional
      transcription initiation complex on a TATA-box-containing mRNA promoter has been 
      well characterized and involves the ordered assembly of a number of general
      transcription factors (GTFs), all of which have been either cloned or purified to
      near homogeneity. In the human RNA polymerase II snRNA promoters, a single
      element, the proximal sequence element (PSE), is sufficient to direct basal
      levels of transcription in vitro. The PSE is recognized by the basal
      transcription complex SNAPc. SNAPc, which is not required for transcription from 
      mRNA-type RNA polymerase II promoters such as the adenovirus type 2 major late
      (Ad2ML) promoter, is thought to recruit TATA binding protein (TBP) and nucleate
      the assembly of the snRNA transcription initiation complex, but little is known
      about which GTFs other than TBP are required. Here we show that the GTFs IIA,
      IIB, IIF, and IIE are required for efficient RNA polymerase II transcription from
      snRNA promoters. Thus, although the factors that recognize the core elements of
      RNA polymerase II mRNA and snRNA-type promoters differ, they mediate the
      recruitment of many common GTFs.
FAU - Kuhlman, T C
AU  - Kuhlman TC
AD  - Graduate Program in Molecular and Cellular Pharmacology, State University of New 
      York at Stony Brook, Stony Brook, New York 11794, USA.
FAU - Cho, H
AU  - Cho H
FAU - Reinberg, D
AU  - Reinberg D
FAU - Hernandez, N
AU  - Hernandez N
LA  - eng
GR  - R01 GM038810/GM/NIGMS NIH HHS/United States
GR  - GM38810/GM/NIGMS NIH HHS/United States
GR  - R01 GM037120/GM/NIGMS NIH HHS/United States
GR  - GM37120/GM/NIGMS NIH HHS/United States
GR  - R37 GM037120/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Ribonucleoprotein, U1 Small Nuclear)
RN  - 0 (Ribonucleoprotein, U4-U6 Small Nuclear)
RN  - 0 (Transcription Factor TFIIA)
RN  - 0 (Transcription Factor TFIIB)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
RN  - 0 (transcription factor TFIIE)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 2.7.7.6 (RNA Polymerase III)
RN  - EC 3.6.4.12 (transcription factor TFIIF)
SB  - IM
MH  - Animals
MH  - HeLa Cells
MH  - Humans
MH  - *Promoter Regions, Genetic
MH  - RNA Polymerase II/*genetics
MH  - RNA Polymerase III/genetics
MH  - *RNA, Small Nuclear
MH  - Rabbits
MH  - Ribonucleoprotein, U1 Small Nuclear/*genetics
MH  - Ribonucleoprotein, U4-U6 Small Nuclear/genetics
MH  - Transcription Factor TFIIA
MH  - Transcription Factor TFIIB
MH  - Transcription Factors/genetics/*metabolism
MH  - *Transcription Factors, TFII
MH  - *Transcription, Genetic
PMC - PMC84006
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1128/mcb.19.3.2130 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Mar;19(3):2130-41. doi: 10.1128/mcb.19.3.2130.