PMID- 10022869
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 3
DP  - 1999 Mar
TI  - Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the
      c-Jun promoter by transforming growth factor beta.
PG  - 1821-30
AB  - Transcriptional regulation by transforming growth factor beta (TGF-beta) is a
      complex process which is likely to involve cross talk between different DNA
      responsive elements and transcription factors to achieve maximal promoter
      activation and specificity. Here, we describe a concurrent requirement for two
      discrete responsive elements in the regulation of the c-Jun promoter, one a
      binding site for a Smad3-Smad4 complex and the other an AP-1 binding site. The
      two elements are located 120 bp apart in the proximal c-Jun promoter, and each
      was able to independently bind its corresponding transcription factor complex.
      The effects of independently mutating each of these elements were nonadditive;
      disruption of either sequence resulted in complete or severe reductions in
      TGF-beta responsiveness. This simultaneous requirement for two distinct and
      independent DNA binding elements suggests that Smad and AP-1 complexes function
      synergistically to mediate TGF-beta-induced transcriptional activation of the
      c-Jun promoter.
FAU - Wong, C
AU  - Wong C
AD  - Department of Pharmacology and Cancer Biology, Duke University Medical Center,
      Durham, North Carolina 27708, USA.
FAU - Rougier-Chapman, E M
AU  - Rougier-Chapman EM
FAU - Frederick, J P
AU  - Frederick JP
FAU - Datto, M B
AU  - Datto MB
FAU - Liberati, N T
AU  - Liberati NT
FAU - Li, J M
AU  - Li JM
FAU - Wang, X F
AU  - Wang XF
LA  - eng
GR  - DK45756/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (SMAD4 protein, human)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, mouse)
RN  - 0 (Smad4 Protein)
RN  - 0 (Smad4 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Cell Line
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-jun/*genetics
MH  - Rabbits
MH  - Smad3 Protein
MH  - Smad4 Protein
MH  - TATA Box
MH  - Trans-Activators/*metabolism
MH  - Transcription Factor AP-1/*metabolism
MH  - *Transcriptional Activation
MH  - Transforming Growth Factor beta/*metabolism
PMC - PMC83975
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1128/mcb.19.3.1821 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Mar;19(3):1821-30. doi: 10.1128/mcb.19.3.1821.