PMID- 10022608
OWN - NLM
STAT- MEDLINE
DCOM- 19990423
LR  - 20191024
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 72
IP  - 4
DP  - 1999 Mar 15
TI  - c-Myc transactivation domain-associated kinases: questionable role for map
      kinases in c-Myc phosphorylation.
PG  - 483-91
AB  - We have isolated and characterized cellular kinases which associate with the
      transactivation domain of c-Myc and phosphorylate Ser-62. We demonstrate that
      cellular Map kinases associate with c-Myc under stringent conditions and
      phosphorylate Ser-62. We also find that TPA stimulates the activity of the
      Myc-associated Map kinase to phosphorylate Ser-62. However, we do not observe an 
      increase in Ser-62 phosphorylation in endogenous c-Myc after TPA treatment of
      cells. Since the regulation of the c-Myc-associated Map kinases does not
      correlate with the in vivo regulation of Ser-62 phosphorylation in c-Myc, we
      conclude that Map kinases are not the in vivo kinases for Ser-62. Although Ser-62
      phosphorylation was not affected by TPA, phosphorylation at a different serine
      residue was significantly upregulated by TPA.
FAU - Lutterbach, B
AU  - Lutterbach B
AD  - Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, 
      Tennessee 37232-2175, USA.
FAU - Hann, S R
AU  - Hann SR
LA  - eng
GR  - CA-47399/CA/NCI NIH HHS/United States
GR  - CA-48799/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Phosphopeptides)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - COS Cells
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Mice
MH  - Phosphopeptides/analysis
MH  - Phosphorylation
MH  - Phosphoserine/analysis
MH  - Protein Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-myc/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1999/02/18 03:02
MHDA- 2000/06/20 09:00
CRDT- 1999/02/18 03:02
PHST- 1999/02/18 03:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/18 03:02 [entrez]
AID - 10.1002/(SICI)1097-4644(19990315)72:4<483::AID-JCB4>3.0.CO;2-I [pii]
AID - 10.1002/(sici)1097-4644(19990315)72:4<483::aid-jcb4>3.0.co;2-i [doi]
PST - ppublish
SO  - J Cell Biochem. 1999 Mar 15;72(4):483-91. doi:
      10.1002/(sici)1097-4644(19990315)72:4<483::aid-jcb4>3.0.co;2-i.