PMID- 10022583
OWN - NLM
STAT- MEDLINE
DCOM- 19990507
LR  - 20191102
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 38
IP  - 3
DP  - 1999 Feb 15
TI  - Prevention of neuronal cell death by neural adhesion molecules L1 and CHL1.
PG  - 428-39
AB  - The effects of L1-Fc and CHL1-Fc fusion proteins on neuronal survival were
      investigated. Cerebellar granule neurons of mouse and hippocampal neurons of rat 
      embryo undergo apoptosis when cultured in serum-free medium. Treatment with
      chimeric proteins containing the extracellular domains of the neural adhesion
      molecules L1 or CHL1 fused to the Fc region of human immunoglobulin significantly
      enhanced the survival of neurons. Compared to the control, the percentage of
      surviving neurons increased about 60% and 45% with L1 and CHL1 fusion proteins,
      respectively. A fusion protein containing the extracellular domain of NCAM had no
      effect on survival. The L1 and CHL1 fusion proteins were effective both in
      soluble form or when offered as a substrate, with the maximal effect at about 1
      microg/mL. To explore the intracellular events related to the neuronal survival
      effects of L1-Fc fusion protein, Bcl-2 and c-Jun expression were analyzed by
      Western blotting. The level of Bcl-2 in cerebellar granule neurons was increased 
      by treatment with L1-Fc at both 1 and 5 days of culture. The level of c-Jun was
      not significantly affected at the early time point and was reduced by L1-Fc
      fusion protein after long-term culture. The results demonstrate that the neural
      adhesion molecule L1 and its relative CHL1 are potential neuronal survival
      factors for neurons of the central nervous system. Bcl-2 may serve as one of the 
      intracellular mediators of the neuronal survival effects of L1.
FAU - Chen, S
AU  - Chen S
AD  - Department of Neurobiology, Swiss Federal Institute of Technology, Honggerberg,
      Zurich.
FAU - Mantei, N
AU  - Mantei N
FAU - Dong, L
AU  - Dong L
FAU - Schachner, M
AU  - Schachner M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (CHL1 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chl1 protein, mouse)
RN  - 0 (Leukocyte L1 Antigen Complex)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Adhesion Molecules
MH  - Cell Death/drug effects
MH  - Cells, Cultured
MH  - Cerebellum/cytology/drug effects
MH  - DNA Fragmentation
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Genes, bcl-2/genetics
MH  - Genes, jun/genetics
MH  - Hippocampus/cytology/drug effects
MH  - Leukocyte L1 Antigen Complex
MH  - Membrane Glycoproteins/*pharmacology
MH  - Membrane Proteins
MH  - Mice
MH  - *Neural Cell Adhesion Molecule L1
MH  - Neural Cell Adhesion Molecules/*pharmacology
MH  - Neurons/*drug effects
MH  - *Proteins
EDAT- 1999/02/18 03:02
MHDA- 2000/06/20 09:00
CRDT- 1999/02/18 03:02
PHST- 1999/02/18 03:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/18 03:02 [entrez]
AID - 10.1002/(SICI)1097-4695(19990215)38:3<428::AID-NEU10>3.0.CO;2-6 [pii]
AID - 10.1002/(sici)1097-4695(19990215)38:3<428::aid-neu10>3.0.co;2-6 [doi]
PST - ppublish
SO  - J Neurobiol. 1999 Feb 15;38(3):428-39. doi:
      10.1002/(sici)1097-4695(19990215)38:3<428::aid-neu10>3.0.co;2-6.