PMID- 10022524
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20131121
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 72
IP  - 3
DP  - 1999 Mar 1
TI  - Identification and characterization of a novel protein that regulates RNA-protein
      interaction.
PG  - 435-44
AB  - In a previous study [Nachaliel et al., 1993], we identified an RNA-binding
      protein (RBP) in FTO-2B rat hepatoma cells whose activity was stimulated upon the
      dissociation of a protein factor. We report in this article that the RBP is a
      complex protein of about 400 kDa, composed of RNA-binding subunit(s) (RBS), and
      regulatory subunit(s) (RS). We purified the RS to near-homogeneity (Mr
      approximately 25,000) and determined the amino acid sequence of a peptide derived
      from RS. On the basis of this sequence information, the cDNA for RS was obtained.
      Recombinant RS protein expressed in Escherichia coli had the capacity to bind RBS
      and inhibit its RNA-binding activity. The cDNA contains the complete coding
      sequence because the recombinant protein has the same electrophoretic mobility as
      that of the native RS in SDS-polyacrylamide gels. Sequence comparison showed that
      RS is almost identical to DJ-1, a recently discovered protein with an oncogenic
      potential, and CAP1, a rat sperm protein. However, the protein does not contain
      any known motifs that can provide a clue as to its exact function. Indirect
      immunofluorescence analyses showed that in addition to the cytoplasm, where RS is
      associated with microtubular filaments, the polypeptide is localized to the cell 
      nucleus. The possible role of RS is discussed.
FAU - Hod, Y
AU  - Hod Y
AD  - Prostate Cancer Research Center, Department of Urology, State University of New
      York, Stony Brook 11794-8093, USA. yhod@surg.som.sunysb.edu
FAU - Pentyala, S N
AU  - Pentyala SN
FAU - Whyard, T C
AU  - Whyard TC
FAU - El-Maghrabi, M R
AU  - El-Maghrabi MR
LA  - eng
GR  - RR02427/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tubulin)
RN  - Z01IVE25KI (Demecolcine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Nucleus/chemistry
MH  - Demecolcine/pharmacology
MH  - Genetic Vectors
MH  - Microscopy, Fluorescence
MH  - RNA-Binding Proteins/chemistry/*physiology
MH  - Rats
MH  - Recombinant Proteins
MH  - Tubulin/pharmacology
EDAT- 1999/02/18 03:02
MHDA- 2000/06/20 09:00
CRDT- 1999/02/18 03:02
PHST- 1999/02/18 03:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/18 03:02 [entrez]
AID - 10.1002/(SICI)1097-4644(19990301)72:3<435::AID-JCB12>3.0.CO;2-H [pii]
PST - ppublish
SO  - J Cell Biochem. 1999 Mar 1;72(3):435-44.