PMID- 10022502
OWN - NLM
STAT- MEDLINE
DCOM- 19990503
LR  - 20071114
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 72
IP  - 2
DP  - 1999 Feb 1
TI  - Integrin alpha3beta1 expressed by human colon cancer cells is a major carrier of 
      oncodevelopmental carbohydrate epitopes.
PG  - 189-209
AB  - Oncodevelopmental carbohydrate epitopes are a common feature of human colorectal 
      carcinoma, yet their biological significance remains unclear. We have shown
      previously that monoclonal antibody (MAb) 3A7, which recognizes a determinant on 
      type 2 chain blood group A and B oligosaccharides, detects oncodevelopmental
      changes in azoxymethane-induced rat colon tumors and some human colon cancer cell
      lines. (Laferte S et al. [19951 J. Cell. Biochem. 57:101-119). In this study, we 
      set out to purify gp140, the major glycoprotein carrier of the 3A7 epitope
      expressed by human colon cancer cells, as a first step towards elucidating the
      contribution of the 3A7 epitope and its major glycoprotein carrier to colon
      cancer progression. To this end, gp140 was purified from HT29 cells and used for 
      the preparation of polypeptide-specific monoclonal antibodies. Five monoclonal
      antibodies (7A8, 7B11, 8C7, 8H7, and 11D4) immunoprecipitated a
      3A7-immunoreactive glycoprotein complex of 140 kDa which was subsequently
      identified by partial protein sequencing as alpha3beta1 integrin. Flow cytometric
      analysis of Chinese hamster ovary (CHO) cells expressing different human integrin
      chains revealed that MAbs 7A8 and 7B11 detect the alpha3 integrin subunit whereas
      MAbs 8C7 and 8H7 detect the beta1 integrin subunit. MAb 11D4, which did not bind 
      to any of the CHO transfectants, detected type 2 chain blood group A determinant.
      Flow cytometric analysis of a panel of human colon carcinoma cell lines obtained 
      from blood group A, AB, or B individuals revealed a direct correlation between
      cell-surface expression of the 3A7 epitope and alpha3 integrin subunit,
      suggesting that alpha3beta1 integrin is a preferred target of the 3A7 epitope in 
      colon cancer cells. Using lectins and glycosidases to examine further the
      carbohydrate structure of alpha3beta1 integrin, we demonstrated that it is a
      sialoglycoprotein containing both N- and O-linked oligosaccharides. In addition, 
      both alpha3 and beta1 integrin subunits express beta1-6 branched Asn-linked
      oligosaccharides and short poly-N-acetyllactosamine units (Galbeta1-4GlcNAc-R; n 
      < or = 3), glycans previously implicated in cancer metastasis.Thus, alpha3beta1
      integrin expressed by human colon carcinoma cells is a major carrier of
      oncodevelopmental carbohydrate epitopes whose presence may modulate tumor cell
      adhesion, migration, and/or invasion.
FAU - Prokopishyn, N L
AU  - Prokopishyn NL
AD  - Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
FAU - Puzon-McLaughlin, W
AU  - Puzon-McLaughlin W
FAU - Takada, Y
AU  - Takada Y
FAU - Laferte, S
AU  - Laferte S
LA  - eng
GR  - GM47157/GM/NIGMS NIH HHS/United States
GR  - GM49899/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Carbohydrates)
RN  - 0 (Epitopes)
RN  - 0 (GP 140)
RN  - 0 (Glycoproteins)
RN  - 0 (Integrin alpha3beta1)
RN  - 0 (Integrins)
RN  - 0 (Oligosaccharides)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Blotting, Western
MH  - CHO Cells
MH  - Carbohydrates/physiology
MH  - Colonic Neoplasms/chemistry
MH  - Cricetinae
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/physiology
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Glycoproteins/isolation & purification
MH  - HT29 Cells
MH  - Humans
MH  - Integrin alpha3beta1
MH  - Integrins/*metabolism
MH  - Mice
MH  - Oligosaccharides/chemistry
MH  - Peptide Mapping
MH  - Precipitin Tests
MH  - Tumor Cells, Cultured
EDAT- 1999/02/18 03:02
MHDA- 2000/06/20 09:00
CRDT- 1999/02/18 03:02
PHST- 1999/02/18 03:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/18 03:02 [entrez]
AID - 10.1002/(SICI)1097-4644(19990201)72:2<189::AID-JCB4>3.0.CO;2-N [pii]
PST - ppublish
SO  - J Cell Biochem. 1999 Feb 1;72(2):189-209.