PMID- 10022450
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20181201
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 84
IP  - 2
DP  - 1999 Feb
TI  - Pituitary tumor transforming gene (PTTG) expression in pituitary adenomas.
PG  - 761-7
AB  - We recently cloned a novel pituitary tumor transforming gene (PTTG). Here we
      report PTTG expression in human pituitary adenomas and in normal pituitary
      tissue. In situ hybridization revealed PTTG expression in nonfunctioning and in
      GH-secreting adenomas but not in normal pituitary tissue. Using a more sensitive 
      detection method, RT-PCR, low level PTTG expression was detected in normal
      pituitary. However, when expression levels in normal pituitary tissue were
      compared with those in 54 pituitary tumors using comparative reverse
      transcription polymerase chain reaction (RT-PCR), we found that most tumor
      samples expressed higher levels of PTTG. More than 50% PTTG increases were
      observed in 23 of 30 nonfunctioning pituitary tumors, all 13 GH-producing tumors,
      9 of 10 prolactinomas, and 1 ACTH-secreting tumor, with more than 10-fold
      increases evident in some tumors. Furthermore, higher PTTG expression (P = 0.03) 
      was observed in hormone-secreting tumors that had invaded the sphenoid bone
      (stages III and IV; 95% CI 3.118-9.715) compared with hormone-secreting tumors
      that were confined to the pituitary fossa (stages I and II; 95% CI 1.681-3.051). 
      Therefore, PTTG abundance is a molecular marker for invasiveness in
      hormone-secreting pituitary tumors. The ubiquitous and prevalent expression of
      pituitary adenoma PTTG suggests that PTTG plays a role in pituitary tumorigenesis
      and invasiveness.
FAU - Zhang, X
AU  - Zhang X
AD  - Cedars-Sinai Research Institute-University of California School of Medicine, Los 
      Angeles 90048, USA.
FAU - Horwitz, G A
AU  - Horwitz GA
FAU - Heaney, A P
AU  - Heaney AP
FAU - Nakashima, M
AU  - Nakashima M
FAU - Prezant, T R
AU  - Prezant TR
FAU - Bronstein, M D
AU  - Bronstein MD
FAU - Melmed, S
AU  - Melmed S
LA  - eng
GR  - DK 50238/DK/NIDDK NIH HHS/United States
GR  - DK 7682/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Securin)
RN  - 0 (pituitary tumor-transforming protein 1, human)
RN  - 12629-01-5 (Human Growth Hormone)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
SB  - AIM
SB  - IM
MH  - Adenoma/*genetics/metabolism/pathology
MH  - Adrenocorticotropic Hormone/metabolism
MH  - Adult
MH  - Aged
MH  - Female
MH  - *Gene Expression
MH  - Human Growth Hormone/metabolism
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*genetics
MH  - Pituitary Neoplasms/*genetics/metabolism/pathology
MH  - Polymerase Chain Reaction
MH  - Prolactinoma/genetics
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Securin
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1210/jcem.84.2.5432 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1999 Feb;84(2):761-7. doi: 10.1210/jcem.84.2.5432.