PMID- 10022429
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20181201
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 84
IP  - 2
DP  - 1999 Feb
TI  - Cerebellin enhances in vitro secretory activity of human adrenal gland.
PG  - 632-5
AB  - Cerebellin is a 16-amino acid peptide, originally isolated from rat cerebellum,
      whose presence has been recently demonstrated in the human adrenal glands and
      especially in medullary chromaffin cells. Cerebellin concentration dependently
      increased basal catecholamine (norepinephrine and epinephrine) release by human
      adrenal slices, containing medullary chromaffin tissue, minimal and maximal
      effective concentrations being 10(-9) and 10(-7) mol/L. Cerebellin (10(-7) mol/L)
      markedly enhanced cAMP release by adrenal slices, and the protein kinase A
      inhibitor H-89 (10(-5) mol/L) blocked catecholamine response to cerebellin.
      Cerebellin did not affect basal steroid secretion of dispersed human
      adrenocortical cells, but it concentration dependently increased aldosterone and 
      cortisol production by adrenal slices. Again minimal and maximal effective
      concentrations were 10(-9) and 10(-7) mol/L. Aldosterone and cortisol responses
      to 10(-7) mol/L cerebellin was suppressed by both the beta-adrenoceptor
      antagonist l-alprenolol (10(-6) mol/L) and H-89 (10(-5) mol/L). Collectively, the
      present findings allow us to conclude that 1) cerebellin exerts a sizable
      secretagogue action on both cortex and medulla of human adrenals; 2) the peptide 
      directly stimulates catecholamine release via the adenylate cyclase/protein
      kinase A-dependent signaling pathway; and 3) the mechanism underlying the
      adrenocortical stimulatory effect of cerebellin is indirect and probably involves
      the release of catecholamines, which in turn, acting in a paracrine manner,
      enhance steroid-hormone secretion.
FAU - Mazzocchi, G
AU  - Mazzocchi G
AD  - Department of Human Anatomy, University of Padua, Italy.
FAU - Andreis, P G
AU  - Andreis PG
FAU - De Caro, R
AU  - De Caro R
FAU - Aragona, F
AU  - Aragona F
FAU - Gottardo, L
AU  - Gottardo L
FAU - Nussdorfer, G G
AU  - Nussdorfer GG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Adrenergic alpha-Antagonists)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoquinolines)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Sulfonamides)
RN  - 4964P6T9RB (Aldosterone)
RN  - 877K5MQ27W (Alprenolol)
RN  - 94071-26-8 (cerebellin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - WI4X0X7BPJ (Hydrocortisone)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Adrenal Cortex/drug effects/metabolism
MH  - Adrenal Glands/*drug effects/*metabolism
MH  - Adrenal Medulla/drug effects/metabolism
MH  - Adrenergic alpha-Antagonists/pharmacology
MH  - Adult
MH  - Aldosterone/metabolism
MH  - Alprenolol/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors
MH  - Enzyme Inhibitors/pharmacology
MH  - Epinephrine/metabolism
MH  - Humans
MH  - Hydrocortisone/metabolism
MH  - In Vitro Techniques
MH  - Isoquinolines/pharmacology
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Norepinephrine/metabolism
MH  - *Sulfonamides
EDAT- 1999/02/18 00:00
MHDA- 1999/02/18 00:01
CRDT- 1999/02/18 00:00
PHST- 1999/02/18 00:00 [pubmed]
PHST- 1999/02/18 00:01 [medline]
PHST- 1999/02/18 00:00 [entrez]
AID - 10.1210/jcem.84.2.5462 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1999 Feb;84(2):632-5. doi: 10.1210/jcem.84.2.5462.