doc_id sent_index relation_id relation trigger trigger_offset arg_num arg_base_np arg_protein arg_domain arg_site arg_sugar PSource SiteSource NProtein NID SiteName sent_text 2129367 10 3 gly presence 1299:1306 arg2 protein Z AND the unique trisaccharide structure protein Z the unique trisaccharide structure Cterm protein Z The presence of the unique trisaccharide structure in factors VII, IX and protein Z leads us to anticipate its biological role in the tissue factor pathway. 2129367 10 3 gly presence 1299:1306 arg1 factors VII AND the unique trisaccharide structure factors VII, IX the unique trisaccharide structure PUBTATOR factors VII, IX 2155 The presence of the unique trisaccharide structure in factors VII, IX and protein Z leads us to anticipate its biological role in the tissue factor pathway. 22750213 3 41 gly glycosylation 492:504 arg1 T T Cterm T P22303 The glycosylation of AChE(T) is known to be required for its proper assembly and trafficking; however, the role of PRiMA glycosylation in the oligomer assembly has not been revealed. 22750213 3 41 gly glycosylation 492:504 arg1 AChE AChE PUBTATOR AChE P22303 The glycosylation of AChE(T) is known to be required for its proper assembly and trafficking; however, the role of PRiMA glycosylation in the oligomer assembly has not been revealed. 9572875 2 32 gly nonglycosylated 386:400 arg1 Glycosylated and nonglycosylated recombinant human IGFBP-6 Glycosylated and nonglycosylated recombinant human IGFBP-6 PUBTATOR IGFBP-6 3489 Glycosylated and nonglycosylated recombinant human IGFBP-6, expressed in Chinese hamster ovary cells and Escherichia coli, respectively, were purified using IGF-II affinity chromatography and reverse-phase medium-pressure chromatography. 16763549 0 13 gly neuropilin-1 34:45 arg1 Glycosaminoglycan modification neuropilin-1 Glycosaminoglycan modification OGER neuropilin-1 O14786 Glycosaminoglycan modification of neuropilin-1 modulates VEGFR2 signaling. 16763549 0 31 gly modification 18:29 arg1 neuropilin-1 AND Glycosaminoglycan modification neuropilin-1 Glycosaminoglycan modification OGER neuropilin-1 O14786 Glycosaminoglycan modification of neuropilin-1 modulates VEGFR2 signaling. 17715132 6 17 gly glycosylation 788:800 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 The glycosylation of Pannexin1 at its extracellular surface makes it unlikely that two oligomers could dock to form an intercellular channel. 19276170 3 54 gly COMP 612:615 arg1 the type 3 repeats COMP the type 3 repeats OGER COMP P49747 In this study, we determined the structure of a recombinant protein that contains the last epidermal growth factor repeat, the type 3 repeats and the C-terminal domain (CTD) of COMP to 3.15-A resolution limit by X-ray crystallography. 1544894 7 69 gly has 1145:1147 arg1 human factor VII AND O-linked fucose human factor VII O-linked fucose OGER factor VII P08709 It has been recently reported that the first EGF domain of human factor VII has O-linked fucose at the equivalent position (Ser-60) (Bjoern, S., Foster, D. C., Thim, L., Wiberg, F. C., Christensen, M., Komiyama, Y., Pedersen, A. H., and Kisiel, W. (1991) J. Biol. 2498325 0 83 gly Glycosylation 0:12 arg1 human apolipoprotein E human apolipoprotein E PUBTATOR apolipoprotein E 348 Glycosylation of human apolipoprotein E. 10978165 10 52 gly glycosylated 1253:1264 arg1 glycosylated HCC-1 HCC-1 (1-74 PUBTATOR HCC-1 (1-74 6358 Our data imply that HCC-1 (1-74), HCC-1 (3-74), HCC-1 (4-74) and glycosylated HCC-1 (1-74) circulate in human blood. 11706042 1 24 gly multi-protein 141:153 arg1 The lipopolysaccharide (LPS) receptor multi The lipopolysaccharide (LPS) receptor OGER multi The lipopolysaccharide (LPS) receptor is a multi-protein complex that consists of at least three proteins, CD14, TLR4, and MD-2. 21752865 7 28 gly N-glycosylated 1245:1258 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 These data indicate that BRI2 is N-glycosylated at Asn170. 22448645 0 64 gly glycosylation 50:62 arg1 the human serotonin 5-HT₇a receptor the human serotonin 5-HT₇a receptor Cterm 5-HT₇a Biochemical and pharmacological study of N-linked glycosylation of the human serotonin 5-HT₇a receptor. 3264725 9 2 gly VIIa 1858:1861 arg1 the overall carbohydrate compositions factor VIIa the overall carbohydrate compositions Cterm factor VIIa Besides minor differences in the sialic acid and fucose contents, the overall carbohydrate compositions were nearly identical in recombinant factor VIIa and human plasma factor VIIa. 3264725 9 75 gly VIIa 1829:1832 arg1 the overall carbohydrate compositions factor VIIa the overall carbohydrate compositions Cterm factor VIIa Besides minor differences in the sialic acid and fucose contents, the overall carbohydrate compositions were nearly identical in recombinant factor VIIa and human plasma factor VIIa. 22448645 3 13 gly N-glycosylated 547:560 arg1 an N-glycosylated form an N-glycosylated form OGER N-glycosylated form of the 5-HT(7 P34969 Western blot analysis of HEK293T cells transiently expressing the 5-HT(7(a)) receptor in the presence of tunicamycin gave rise to a band shift, indicating the existence of an N-glycosylated form of the 5-HT(7(a)) receptor. 18508581 3 1 gly glycosylation 497:509 arg1 EpCAM EpCAM PUBTATOR EpCAM 4072 We have uncovered differential glycosylation of EpCAM as a means to discriminate normal from malignant tissues. 16201406 1 18 gly glycoprotein 203:214 arg1 Human lactoferrin Human lactoferrin OGER Human lactoferrin P02788 Human lactoferrin (hLF) is an iron-binding glycoprotein involved in the host defence against infection and excessive inflammation. 20511397 7 32 gly sialylated 1385:1394 arg1 cell-derived apoE cell-derived apoE PUBTATOR apoE 348 Comparison of plasma and cellular/secreted apoE from the same donor confirmed that cell-derived apoE is more extensively sialylated than plasma apoE. 9767079 7 33 gly N-glycosylation 1274:1288 arg1 human RFC human RFC PUBTATOR RFC 6573 Collectively, our results demonstrate that N-glycosylation of human RFC plays no significant role in either transport function or membrane targeting. 17286803 3 61 gly glycosylation 351:363 arg1 CLN3 CLN3 OGER CLN3 Q13286 We now examined the role of glycosylation and the C-terminal CAAX motif in lysosomal transport of CLN3 in non-neuronal and neuronal cells. 7918467 7 32 part_of apoA-II 810:816 arg1 Cys6 apoA-II Cys6 PUBTATOR AminoAcid apoA-II 336 Cys6 Using a combination of manual Edman degradations and mass spectrometric analysis on a purified cluster of chymotryptic fragments, we identified an intramolecular disulfide bridge between Cys8 and Cys114 and an intermolecular bridge between Cys116 of apoD and Cys6 of apoA-II. 7918467 7 21 part_of apoD 793:796 arg1 Cys114 apoD Cys8 and Cys114 PUBTATOR AminoAcid apoD 347 Cys8 and Cys114 Using a combination of manual Edman degradations and mass spectrometric analysis on a purified cluster of chymotryptic fragments, we identified an intramolecular disulfide bridge between Cys8 and Cys114 and an intermolecular bridge between Cys116 of apoD and Cys6 of apoA-II. 11152678 2 20 gly N-glycosylated 434:447 arg1 pro-BDNF pro-BDNF PUBTATOR AminoAcid BDNF 627 Metabolic labeling, immunoprecipitation, and SDS-polyacrylamide gel electrophoresis reveal that pro-BDNF is generated as a 32-kDa precursor that is N-glycosylated and glycosulfated on a site, within the pro-domain. 15628971 7 83 gly glycosylation 1461:1473 arg1 recombinant C4ST recombinant C4ST PUBTATOR C4ST 314694 In addition, the N-linked oligosaccharide at the C-terminal region appears to affect the glycosylation pattern of recombinant C4ST; a broad protein band of the wildtype protein resulting from microheterogeneity of N-linked oligosaccharides disappeared and four discrete protein bands with different numbers of N-linked oligosaccharides appeared when the N-linked oligosaccharide at the C-terminal region was deleted. 18070108 8 23 part_of BMP-6 1248:1252 arg1 Asn73 BMP-6 Asn73 PUBTATOR AminoAcid BMP-6 654 Asn73 Further studies investigating the interaction of BMP-6 with different ectodomains of type I receptors revealed that N-glycosylation at Asn73 of BMP-6 in the wrist epitope is crucial for recognition by the activin receptor type I. 8626443 3 39 gly unglycosylated 510:523 arg1 unglycosylated ACET unglycosylated ACET Cterm ACET Similarly, unglycosylated ACET synthesized in HeLa cells, by using a cDNA in which all five potential N-glycosylation sites had been mutated, was inactive and rapidly degraded. 18467335 12 69 gly PCI 1565:1567 arg1 the N-linked glycans PCI the N-linked glycans OGER PCI P05154 These results thus demonstrate that the N-linked glycans and the N-terminal region of blood-derived PCI in different ways affect the cofactor-enhanced rates of thrombin inhibition and provide information on the mechanisms by which this may be achieved. 2668275 12 14 gly contain 1566:1572 arg1 STS AND mannose 6-phosphate residues STS mannose 6-phosphate residues OGER STS P08842 In spite of its similarity with these two lysosomal sulfatases, STS does not contain mannose 6-phosphate residues and is transported to lysosomes by a mannose 6-phosphate receptor-independent mechanism. 16698036 1 75 gly beta-hexosaminidase 147:165 arg1 Hex A beta-hexosaminidase Hex A OGER beta-hexosaminidase Lysosomal beta-hexosaminidase A (Hex A) is essential for the degradation of GM2 gangliosides in the central and peripheral nervous system. 10756055 4 49 gly glycosylated 873:884 arg1 cell type-dependent glycosylated CXCR4 cell type-dependent glycosylated CXCR4 PUBTATOR CXCR4 7852 These results may have far-reaching implications for the differential recognition of cell type-dependent glycosylated CXCR4 by HIV-1 isolates and their evolution in vivo. 12970363 10 21 gly monoglycosylated 1432:1447 arg1 the monoglycosylated TRPC3 the monoglycosylated TRPC3 PUBTATOR TRPC3 7222 Elimination of the e2 glycosylation site, missing in the monoglycosylated TRPC3, was sufficient to convert the tightly receptor-regulated TRPC6 into a constitutively active channel, displaying functional characteristics of TRPC3. 26536155 6 38 gly glycosylation 1501:1513 arg1 human IgG3 human IgG3 OGER IgG3 P01860 The approach was evaluated on glycoprotein standards and also applied to investigate the glycosylation of human IgG3 providing details on the hitherto uncharacterized glycosylation site Asn392 of the CH3 domain. 20044576 3 18 gly glycan 562:567 arg1 recombinant alpha-DG DG, glycan PUBTATOR DG, 1605 Using mass spectrometry- and nuclear magnetic resonance (NMR)-based structural analyses, we identified a phosphorylated O-mannosyl glycan on the mucin-like domain of recombinant alpha-DG, which was required for laminin binding. 8489250 1 0 gly glycoprotein 235:246 arg1 recombinant human differentiation-stimulating factor recombinant human differentiation-stimulating factor OGER differentiation-stimulating factor P15018 This report describes the post-translational modifications of recombinant human differentiation-stimulating factor, a 180-residue glycoprotein that is secreted from transfected Chinese hamster ovary cells. 23050552 1 55 gly glycoprotein 132:143 arg1 Human fibrinogen Human fibrinogen PUBTATOR Human fibrinogen 2244 Human fibrinogen is a 340 kDa, soluble plasma glycoprotein composed of paired sets of three subunits (α, β, γ). 24692546 3 6 gly glycosylated 516:527 arg1 fully glycosylated FSH fully glycosylated FSH OGER FSH The model predicts that FSHR binds Asnα(52)-deglycosylated FSH at a 3-fold higher capacity than fully glycosylated FSH. 24692546 3 14 gly -deglycosylated 457:471 arg1 Asnα(52)-deglycosylated FSH Asnα(52)-deglycosylated FSH OGER FSH The model predicts that FSHR binds Asnα(52)-deglycosylated FSH at a 3-fold higher capacity than fully glycosylated FSH. 3571235 4 1 gly sialoglycoprotein 624:640 arg1 glycophorin A glycophorin A PUBTATOR glycophorin A 2993 The amino acid sequence of the intramembranous domain (residues 36-71) of glycophorin B was determined and found to be similar to that of the hydrophobic region of the major sialoglycoprotein (glycophorin A). 1883960 0 91 gly glycosylation 41:53 arg1 human interleukin-6 human interleukin-6 PUBTATOR interleukin-6 3569 Marked cell-type-specific differences in glycosylation of human interleukin-6. 2498325 12 32 gly glycosylation 1825:1837 arg1 apoE apoE PUBTATOR apoE P02649 The transfected ldlD cells also secreted high levels of apoE even in the absence of glycosylation, which confirms that glycosylation is not essential for secretion of apoE. 19004833 8 26 gly N-glycosylation 1278:1292 arg1 DS-epimerase 1 DS-epimerase 1 OGER DS-epimerase 1 Q9UL01 In addition, we show that proper N-glycosylation of DS-epimerase 1 is required for enzyme activity. 1517205 0 64 gly has 16:18 arg1 Human factor IX AND a tetrasaccharide Human factor IX a tetrasaccharide OGER factor IX P00740 Human factor IX has a tetrasaccharide O-glycosidically linked to serine 61 through the fucose residue. 18764929 4 74 gly glycosylated 795:806 arg1 the full-length and glycosylated beta-DG the full-length and glycosylated beta-DG Cterm DG 1605 In this study, we identify a functional nuclear localization signal within beta-DG and show that, in addition to associating with alpha-DG at the cell surface, the full-length and glycosylated beta-DG autonomously traffics to the cytoplasm and nucleoplasm in a process that occurs independent of alpha-DG ligand binding. 8636209 8 53 gly glycosylation 1015:1027 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 In contrast, treatment of T cells with tunicamycin suggested that N-linked glycosylation of CD3 delta is required for TCR assembly. 8636209 8 53 gly glycosylation 1015:1027 arg1 CD3 delta CD3 delta PUBTATOR CD3 delta 915 In contrast, treatment of T cells with tunicamycin suggested that N-linked glycosylation of CD3 delta is required for TCR assembly. 7574684 2 54 gly contains 508:515 arg1 trkB AND 33.3% carbohydrate moieties trkB 33.3% carbohydrate moieties PUBTATOR trkB 4915 The extracellular domain contains 398 amino acids and has a molecular weight of 60.6 kDa according to laser desorption mass spectrometry, indicating that the extracellular domain of trkB contains 33.3% carbohydrate moieties. 19141282 3 9 gly unglycosylated 454:467 arg1 unglycosylated IL-7Ralpha unglycosylated IL-7Ralpha PUBTATOR IL-7Ralpha 3575 IL-7 binds glycosylated IL-7Ralpha 300-fold more tightly than unglycosylated IL-7Ralpha, and the enhanced affinity is attributed primarily to an accelerated on rate. 19141282 3 31 gly glycosylated 403:414 arg1 glycosylated IL-7Ralpha glycosylated IL-7Ralpha PUBTATOR IL-7Ralpha 3575 IL-7 binds glycosylated IL-7Ralpha 300-fold more tightly than unglycosylated IL-7Ralpha, and the enhanced affinity is attributed primarily to an accelerated on rate. 2498325 7 68 part_of Apolipoprotein 945:958 arg1 Thr194----Ala Apolipoprotein E Thr194----Ala PUBTATOR AminoAcid Apolipoprotein E 348 Thr194 Apolipoprotein E(Thr194----Ala) was secreted exclusively as the asialo isoform, confirming that Thr194 is the site of carbohydrate attachment in these cells and indicating that glycosylation of apoE is not essential for secretion. 19855092 10 88 gly desialylated 1724:1735 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin 11450 Finally, after chronic administration in adiponectin(-/-) mice steady-state levels of desialylated adiponectin were lower than control adiponectin and failed to recapitulate the improvements in glucose and insulin tolerance tests observed with control adiponectin. 22750213 5 17 part_of PRiMA 859:863 arg1 the asparagine-43 PRiMA the asparagine-43 PUBTATOR SpecificSite PRiMA 170952 asparagine-43 By using site-directed mutagenesis, the asparagine-43 was identified to be the N-linked glycosylation site of PRiMA. 8172892 3 62 gly contained 487:495 arg1 AP beta AND galactosamine AP beta galactosamine PUBTATOR AP beta 2028 Amino acid analysis showed that AP alpha, but not AP beta and AP gamma, contained galactosamine in addition to glucosamine, thereby suggesting the presence of an O-linked sugar chain(s) in the molecule of AP alpha. 8172892 3 62 gly contained 487:495 arg1 AP alpha AND galactosamine AP alpha galactosamine PUBTATOR AP alpha 2028 Amino acid analysis showed that AP alpha, but not AP beta and AP gamma, contained galactosamine in addition to glucosamine, thereby suggesting the presence of an O-linked sugar chain(s) in the molecule of AP alpha. 3264725 1 7 gly glycoprotein 186:197 arg1 Blood coagulation factor VII Blood coagulation factor VII OGER coagulation factor VII P08709 Blood coagulation factor VII is a vitamin K dependent glycoprotein which in its activated form, factor VIIa, participates in the coagulation process by activating factor X and/or factor IX in the presence of Ca2+ and tissue factor. 10871631 0 82 gly glycoproteins 70:82 arg1 Torsin A Torsin A PUBTATOR Torsin A 1861 Torsin A and its torsion dystonia-associated mutant forms are lumenal glycoproteins that exhibit distinct subcellular localizations. 20511397 0 48 gly Glycosylation 0:12 arg1 macrophage-derived human apolipoprotein E macrophage-derived human apolipoprotein E PUBTATOR apolipoprotein E 348 Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290. 20511397 0 55 gly E 73:73 arg1 sialylation apolipoprotein E sialylation PUBTATOR apolipoprotein E 348 Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290. 20511397 0 59 gly sialylation 18:28 arg1 macrophage-derived human apolipoprotein E macrophage-derived human apolipoprotein E PUBTATOR apolipoprotein E 348 Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290. 23723439 8 29 gly glycosylated 1609:1620 arg1 the sulfated α-DG the sulfated α-DG Cterm DG 1605 Furthermore, using an in vitro enzymatic assay system, we demonstrated that the sulfated α-DG by HNK-1ST is no longer glycosylated by LARGE. 1533633 11 23 gly nonglycosylated 1656:1670 arg1 the nonglycosylated, misfolded alpha-subunit the nonglycosylated, misfolded alpha-subunit OGER subunit P06865 The properties of the nonglycosylated, misfolded alpha-subunit were similar to some mutant alpha-subunits in Tay-Sachs disease patients. 9524075 12 50 gly Non-glycosylated 1303:1318 arg1 Non-glycosylated procathepsin S Non-glycosylated procathepsin S Cterm Non-glycosylated procathepsin S 1520 Non-glycosylated procathepsin S was bound to the plasma membrane at 2 degrees C, suggesting an additional sorting motif in the cathepsin S molecule besides the Man-6-phosphate residue. 15014436 2 55 gly TSPs 242:245 arg1 the calcium-binding type 3 (T3) repeats TSPs the calcium-binding type 3 (T3) repeats Cterm TSPs 7057 The most highly conserved region of all TSPs are the calcium-binding type 3 (T3) repeats and the C-terminal globular domain (CTD). 15628971 2 47 gly glycoprotein 335:346 arg1 purified C4ST-1 purified C4ST-1 PUBTATOR C4ST-1 314694 We showed previously that purified C4ST-1 from the culture medium of rat chondrosarcoma cells was a glycoprotein containing approx. 20823119 2 60 gly IgA1 225:228 arg1 galactose (Gal)-deficient hinge region (HR) O-glycans IgA1 galactose (Gal)-deficient hinge region (HR) O-glycans PUBTATOR IgA1 P01876 Aberrantly glycosylated IgA1, with galactose (Gal)-deficient hinge region (HR) O-glycans, plays a pivotal role in the pathogenesis of the disease. 20823119 2 65 gly glycosylated 212:223 arg1 Aberrantly glycosylated IgA1 Aberrantly glycosylated IgA1 PUBTATOR IgA1 P01876 Aberrantly glycosylated IgA1, with galactose (Gal)-deficient hinge region (HR) O-glycans, plays a pivotal role in the pathogenesis of the disease. 3353370 8 64 gly chain 1717:1721 arg1 the leucine-rich tandem repeats alpha chain the leucine-rich tandem repeats PUBTATOR alpha chain 2217 The leucine-rich sequence in the beta chain of GPIb is flanked on both sides by amino acid sequences that are similar to those flanking the leucine-rich tandem repeats of the alpha chain of GPIb and leucine-rich alpha 2-glycoprotein. 20188224 3 46 gly glycoprotein 389:400 arg1 PGRN PGRN PUBTATOR PGRN 2896 PGRN is a glycoprotein, containing five N-glycosylation consensus sequons, three of which fall within granulin domains. 16186819 1 14 gly glycoproteins 111:123 arg1 Thrombospondins Thrombospondins PUBTATOR Thrombospondins 7058 Thrombospondins (THBSs) are secreted glycoproteins that have key roles in interactions between cells and the extracellular matrix. 2498325 2 37 gly glycosylation 96:108 arg1 human apolipoprotein (apo) E human apolipoprotein (apo) E PUBTATOR apolipoprotein (apo) E 348 The glycosylation of human apolipoprotein (apo) E was examined with purified plasma apoE and apoE produced by transfected cell lines. 9524075 8 68 gly non-glycosylated 870:885 arg1 non-glycosylated procathepsin S non-glycosylated procathepsin S Cterm non-glycosylated procathepsin S 1520 In vitro processing of glycosylated as well as of non-glycosylated procathepsin S gave fully active enzymes thus indicating that the oligosaccharide chain was not necessary for proper folding. 7682553 7 25 part_of bikunin 1152:1158 arg1 Ser10 bikunin Ser10 PUBTATOR AminoAcid bikunin 259 Ser10 Biochemical and mass spectrometric analysis of the peptides containing the cross-link indicate that it is mediated by a chondroitin-4-sulfate chain that originates from a typical O-glycosidic link to Ser10 of bikunin. 10397151 10 55 gly RIIa 1294:1297 arg1 core mannose oligosaccharide side chains Fc gamma RIIa core mannose oligosaccharide side chains PUBTATOR Fc gamma RIIa 2212 Electrospray ionisation mass spectrometry (ESMS) indicate that the N-glycans of baculovirus derived Fc gamma RIIa are core mannose oligosaccharide side chains. 10397151 10 55 gly RIIa 1294:1297 arg1 the N-glycans Fc gamma RIIa the N-glycans PUBTATOR Fc gamma RIIa 2212 Electrospray ionisation mass spectrometry (ESMS) indicate that the N-glycans of baculovirus derived Fc gamma RIIa are core mannose oligosaccharide side chains. 22688517 4 43 gly N-glycosylation 470:484 arg1 recombinant human LOX-1 recombinant human LOX-1 PUBTATOR LOX-1 4973 The present study was aimed at elucidating the N-glycosylation of recombinant human LOX-1 with regard to N-glycan profile and N-glycosylation sites. 24927598 4 36 gly deglycosylated 580:593 arg1 ZIP14 ZIP14 PUBTATOR ZIP14 23516 In an attempt to dissect the molecular mechanisms by which iron regulates ZIP14 levels, we found that ZIP14 is endocytosed, extracted from membranes, deglycosylated, and degraded by proteasomes. 3571235 5 26 part_of C 741:741 arg1 residues 49-88 glycophorin C residues 49-88 PUBTATOR SpecificSite glycophorin C 2995 residues 49-88 The amino acid sequence of the hydrophobic domain (residues 49-88) of glycophorin C, that was also determined, agreed completely with the structure recently deduced from cDNA sequencing. 26274980 1 26 gly macro-heterogeneity 506:524 arg1 nCG nCG Cterm nCG 1511 To facilitate such investigations, we here use complementary LC-MS/MS-based N-glycan, N-glycopeptide, and intact glycoprotein profiling to accurately establish the micro- and macro-heterogeneity of nCG from healthy individuals. 21676880 4 34 gly unglycosylated 870:883 arg1 unglycosylated KCNE1 subunits unglycosylated KCNE1 subunits PUBTATOR KCNE1 subunits 3753 Mutations that ablate the co-translational site concomitantly reduce glycosylation at the post-translational site, resulting in unglycosylated KCNE1 subunits that cannot reach the cell surface with their cognate K(+) channel. 17395589 3 50 gly contains 259:266 arg1 ADAMTS13 AND eight thrombospondin type 1 repeats ADAMTS13 eight thrombospondin type 1 repeats PUBTATOR ADAMTS13 11093 ADAMTS13 contains eight thrombospondin type 1 repeats (TSR), seven of which contain a consensus sequence for the direct addition of fucose to the hydroxyl group of serine or threonine. 14699159 9 69 gly glycosylation 1805:1817 arg1 p90ATF6 p90ATF6 Cterm p90ATF6 22926 Because accumulation of underglycosylated proteins in the ER is a potent inducer for the UPR, these studies uncover a novel mechanism whereby the glycosylation status of p90ATF6 can serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the UPR. 15477100 1 67 gly Zn-alpha2-glycoprotein 70:91 arg1 ZAG ZAG PUBTATOR ZAG 563 Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. 15477100 1 67 gly Zn-alpha2-glycoprotein 70:91 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. 24226769 0 65 gly glycosylates 28:39 arg1 Notch Notch PUBTATOR Notch 100037842 The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality. 21606496 2 56 gly glycosylated 276:287 arg1 2B4 2B4 OGER 2B4 Q9BZW8 Here we show that 2B4 is heavily and differentially glycosylated in primary human NK cells and NK cell lines. 1544894 5 18 gly fucosylated 943:953 arg1 factor XII factor XII OGER factor XII P00748 We found that factor XII is fully fucosylated at Thr-90. 12200435 4 46 gly glycoprotein 645:656 arg1 Human Dpl Human Dpl PUBTATOR Human Dpl 23627 Human Dpl appears to be a glycosylphosphatidylinositol-anchored glycoprotein with N- and O-linked sugars. 22809326 8 89 gly glycosylated 1032:1043 arg1 a heavily glycosylated lysosomal membrane protein a heavily glycosylated lysosomal membrane protein OGER glycosylated lysosomal membrane protein Q8WWB7 It is the first high-resolution structure of a heavily glycosylated lysosomal membrane protein. 15657036 5 50 gly type 603:606 arg1 secreted FGE FGE type PUBTATOR FGE 285362 Intracellular FGE contains a high mannose type N-glycan, which is processed to the complex type in secreted FGE. 15657036 5 14 gly contains 530:537 arg1 Intracellular FGE AND a high mannose type N-glycan Intracellular FGE a high mannose type N-glycan PUBTATOR FGE 285362 Intracellular FGE contains a high mannose type N-glycan, which is processed to the complex type in secreted FGE. 8670172 9 73 gly chain 1339:1343 arg1 Asn-18 Asn-18 Asn-18 SpecificSite Asn-18 The N-linked carbohydrate side chain of CD59u (at Asn-18) also displayed considerable heterogeneity. 8670172 9 92 gly CD59u 1348:1352 arg1 The N-linked carbohydrate side chain CD59u The N-linked carbohydrate side chain Cterm CD59u P13987 The N-linked carbohydrate side chain of CD59u (at Asn-18) also displayed considerable heterogeneity. 23527852 0 86 gly glycoforms 42:51 arg1 new apolipoprotein-CIII glycoforms new apolipoprotein-CIII glycoforms PUBTATOR apolipoprotein-CIII 345 Identification of new apolipoprotein-CIII glycoforms with ultrahigh resolution MALDI-FTICR mass spectrometry of human sera. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Thr146 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Thr126 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser144 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Thr126 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser144 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser144 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 1694179 9 28 gly glycosylated 1241:1252 arg1 beta protein C beta protein C OGER protein C P02810 It is asparagine 329 that is not glycosylated in beta protein C since antibodies to a synthetic peptide based on the sequence around this amino acid react only with beta protein C. 17715132 9 40 gly N-glycosylation 1149:1163 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We propose that N-glycosylation of Pannexin1 could be a significant mechanism for regulating the trafficking of these membrane proteins to the cell surface in different tissues. 20511397 4 10 gly sialylation 768:778 arg1 cellular and secreted apoE cellular and secreted apoE PUBTATOR apoE 348 We investigated glycosylation and sialylation of cellular and secreted apoE from primary human macrophages by one- and two-dimensional gel electrophoresis and mass spectrometry. 20511397 4 47 gly glycosylation 750:762 arg1 cellular and secreted apoE cellular and secreted apoE PUBTATOR apoE 348 We investigated glycosylation and sialylation of cellular and secreted apoE from primary human macrophages by one- and two-dimensional gel electrophoresis and mass spectrometry. 20511397 4 72 gly apoE 805:808 arg1 sialylation apoE sialylation PUBTATOR apoE 348 We investigated glycosylation and sialylation of cellular and secreted apoE from primary human macrophages by one- and two-dimensional gel electrophoresis and mass spectrometry. 9030779 0 59 gly N-glycosylation 35:49 arg1 human acid sphingomyelinase human acid sphingomyelinase PUBTATOR acid sphingomyelinase 6609 Functional characterization of the N-glycosylation sites of human acid sphingomyelinase by site-directed mutagenesis. 2498325 11 66 gly sialylated 1689:1698 arg1 multiply sialylated apoE multiply sialylated apoE PUBTATOR apoE P02649 The transfected wild-type cells secreted multiply sialylated apoE. 28060820 2 12 gly N-glycosylation 584:598 arg1 the human sIL-6R the human sIL-6R OGER sIL Q15468 Here, we use liquid chromatography-mass spectrometry to identify an sIL-6R form in human serum that originates from proteolytic cleavage, map its cleavage site between Pro-355 and Val-356, and determine the occupancy of all O- and N-glycosylation sites of the human sIL-6R. 22688517 6 24 gly glycosylation 1145:1157 arg1 recombinant human LOX-1 recombinant human LOX-1 PUBTATOR LOX-1 4973 With this approach, one potential glycosylation site of recombinant human LOX-1 on Asn(139) is readily identified and found to carry heterogeneous complex type N-glycans. 22688517 6 30 gly carry 1238:1242 arg1 recombinant human LOX-1 AND heterogeneous complex type N-glycans recombinant human LOX-1 one potential glycosylation site heterogeneous complex type N-glycans PUBTATOR LOX-1 4973 site With this approach, one potential glycosylation site of recombinant human LOX-1 on Asn(139) is readily identified and found to carry heterogeneous complex type N-glycans. 7681597 4 25 gly containing 827:836 arg1 GPA-2 AND two nonconsecutive GalNAc-Ser/Thr residues GPA-2 the glycopeptide two nonconsecutive GalNAc-Ser/Thr residues OGER GPA-2 P02724 glycopeptide The Tn antigenicity, as assayed by the binding to a monoclonal anti-Tn antibody (MLS 128), was found exclusively in the glycopeptides including three (cluster I) or four (cluster II) consecutive residues of GalNAc-Ser/Thr, whereas the glycopeptide (GPA-2) containing two nonconsecutive GalNAc-Ser/Thr residues had practically no Tn antigenicity. 15003450 3 31 gly glycoprotein 465:476 arg1 the human alpha-GAL glycoprotein the human alpha-GAL glycoprotein OGER GAL glycoprotein Q8N6F7 Here, we present the structure of the human alpha-GAL glycoprotein determined by X-ray crystallography. 18930737 1 26 gly alpha2-glycoprotein 218:236 arg1 zinc alpha2-glycoprotein zinc alpha2-glycoprotein PUBTATOR zinc alpha2-glycoprotein 563 This is the first report on the formation of a complex between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP). 18930737 1 26 gly alpha2-glycoprotein 218:236 arg1 ZAG ZAG PUBTATOR ZAG 563 This is the first report on the formation of a complex between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP). 10066782 0 57 gly N-glycosylation 29:43 arg1 the human interleukin-6 receptor the human interleukin-6 receptor PUBTATOR interleukin-6 receptor 3570 Disulfide bond structure and N-glycosylation sites of the extracellular domain of the human interleukin-6 receptor. 21148085 0 52 gly factor-D 55:62 arg1 Structural determinants vascular endothelial growth factor-D Structural determinants PUBTATOR vascular endothelial growth factor-D 2277 Structural determinants of vascular endothelial growth factor-D receptor binding and specificity. 11406581 5 33 gly glycan 781:786 arg1 IgG IgG glycan Cterm IgG EndoS is required for the activity on IgG, as an isogenic EndoS mutant could not hydrolyze the glycan on IgG. 9572875 3 14 gly glycosylated 659:670 arg1 glycosylated IGFBP-6 glycosylated IGFBP-6 PUBTATOR IGFBP-6 3489 Electrospray ionization mass spectrometry (ESMS) of glycosylated IGFBP-6 revealed considerable heterogeneity of carbohydrate composition. 18491227 8 40 gly E-cadherin 1305:1314 arg1 N-glycans E-cadherin N-glycans OGER E-cadherin P12830 Furthermore, we found that N-glycans of M4 E-cadherin were modified in immature high mannose type, suggesting that it could not depart to Golgi apparatus. 8702538 8 96 gly glycoprotein 1372:1383 arg1 myelin-associated glycoprotein myelin-associated glycoprotein OGER myelin-associated glycoprotein P20916 Site-directed mutagenesis of similar NC(T/S) motifs in the first or second Ig domains of the I-type lectins myelin-associated glycoprotein, and sialoadhesin did not disrupt their ability to mediate sialic acid binding. 20427278 0 75 gly glycosylation 52:64 arg1 the amyloid precursor protein the amyloid precursor protein OGER amyloid precursor protein P05067 The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. 23851396 1 5 gly glycoproteins 145:157 arg1 Folate receptors Folate receptors PUBTATOR Folate receptors (FRα, FRβ and FRγ) 2348 Folate receptors (FRα, FRβ and FRγ) are cysteine-rich cell-surface glycoproteins that bind folate with high affinity to mediate cellular uptake of folate. 10978165 9 13 gly nonglycosylated 1128:1142 arg1 nonglycosylated HCC-1 HCC-1 (1-74 PUBTATOR HCC-1 (1-74 6358 In hemofiltrate approximately 3% of total HCC-1 represents HCC-1 (3-74) and approximately 1% represents HCC-1 (4-74) whereas the major products are nonglycosylated HCC-1 (1-74) and glycosylated HCC-1 (1-74). 10978165 9 18 gly glycosylated 1161:1172 arg1 glycosylated HCC-1 HCC-1 (1-74 PUBTATOR HCC-1 (1-74 6358 In hemofiltrate approximately 3% of total HCC-1 represents HCC-1 (3-74) and approximately 1% represents HCC-1 (4-74) whereas the major products are nonglycosylated HCC-1 (1-74) and glycosylated HCC-1 (1-74). 18930737 0 14 gly alpha2-glycoprotein 59:77 arg1 ZAG ZAG PUBTATOR ZAG 563 Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma. 18930737 0 14 gly alpha2-glycoprotein 59:77 arg1 zinc alpha2-glycoprotein zinc alpha2-glycoprotein PUBTATOR zinc alpha2-glycoprotein 563 Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma. 14981520 9 34 gly glycosylation 1494:1506 arg1 seipin seipin PUBTATOR seipin 26580 The amino acid substitutions N88S and S90L affect glycosylation of seipin and result in aggregate formation leading to neurodegeneration. 14718370 0 31 gly MUC5AC 19:24 arg1 C-Mannosylation MUC5AC C-Mannosylation PUBTATOR MUC5AC P98088 C-Mannosylation of MUC5AC and MUC5B Cys subdomains. 14718370 0 35 gly MUC5B 30:34 arg1 C-Mannosylation MUC5B C-Mannosylation PUBTATOR MUC5B Q9HC84 C-Mannosylation of MUC5AC and MUC5B Cys subdomains. 3840370 13 44 gly 2-glycoprotein 1955:1968 arg1 the non-complement beta 2-glycoprotein I the non-complement beta 2-glycoprotein I OGER beta 2-glycoprotein I P02749 These regions in C4b-binding protein are homologous with the three internal-homology regions that have been reported to be present within the Ba region of the complement enzyme factor B and also to the internal-homology regions found in the non-complement beta 2-glycoprotein I. 18508581 4 12 gly hyperglycosylated 588:604 arg1 EpCAM EpCAM PUBTATOR EpCAM 4072 EpCAM was hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. 15750791 3 4 gly glycosylated 422:433 arg1 Edg-1/S1P1 Edg-1/S1P1 PUBTATOR Edg-1 1901 Our recent novel studies established that Edg-1/S1P1 is glycosylated in its N-terminal extracellular portion and further identified the specific glycosylation site as asparagine 30. 21199866 4 2 gly glycosylated 534:545 arg1 endogenous ABCB6 endogenous ABCB6 PUBTATOR ABCB6 10058 In this study, we show that endogenous ABCB6 is glycosylated in multiple cell types, indicating trafficking through the endoplasmic reticulum (ER), and has only one atypical site for glycosylation (NXC) in its amino terminus. 19683538 6 24 gly glycoprotein 1071:1082 arg1 the alpha-NAGAL glycoprotein the alpha-NAGAL glycoprotein Cterm alpha-NAGAL glycoprotein 4668 To better understand how individual defects in the alpha-NAGAL glycoprotein lead to Schindler disease, we analyzed the effect of disease-causing mutations on the three-dimensional structure. 8670172 9 96 part_of Asn-18 1358:1363 arg1 CD59u CD59u Asn-18 Cterm SpecificSite CD59u P13987 Asn-18 The N-linked carbohydrate side chain of CD59u (at Asn-18) also displayed considerable heterogeneity. 21805521 5 27 gly glycosylated 830:841 arg1 huDKK1 huDKK1 Cterm huDKK1 22943 LC-MS/MS peptide mapping indicates that ∼ 92% of huDKK1 is glycosylated at Asn²²⁵ with three N-linked glycans composed of two biantennary forms with 1 and 2 sialic acid (23% and 60%, respectively), and one triantennary structure with 2 sialic acids (9%). 15750791 5 23 gly N-glycan 769:776 arg1 Edg-1/S1P1 Edg-1 N-glycan PUBTATOR Edg-1 1901 These studies revealed a possible regulatory role for the N-glycan on Edg-1/S1P1 in the dynamics of the receptor, such as its lateral and internal movements within the membrane, in ligand-stimulated mammalian cells. 17711303 5 65 gly contains 876:883 arg1 the hKOR AND O-linked glycan the hKOR O-linked glycan PUBTATOR hKOR 4986 FLAG-hKOR was reduced to lower Mr bands by neuraminidase and O-glycosidase, indicating that the hKOR contains O-linked glycan. 16186819 2 5 gly glycosylated 269:280 arg1 human THBS2 human THBS2 PUBTATOR THBS2 7058 Here, we describe the 2.6-A-resolution crystal structure of the glycosylated signature domain of human THBS2, which includes three epidermal growth factor-like modules, 13 aspartate-rich repeats and a lectin-like module. 23139753 8 6 gly N-glycosylated 886:899 arg1 HE-4 HE-4 PUBTATOR HE-4 10406 Moreover, HE-4 is N-glycosylated and highly stable on a wide range of pH and temperature. 21606496 5 39 gly 2B4 641:643 arg1 sialylation 2B4 sialylation OGER 2B4 Q9BZW8 In contrast, sialylation of 2B4 has a negative impact on ligand binding, as the interaction between 2B4 and CD48 is increased after the removal of sialic acids. 21606496 5 54 gly sialylation 626:636 arg1 2B4 2B4 OGER 2B4 Q9BZW8 In contrast, sialylation of 2B4 has a negative impact on ligand binding, as the interaction between 2B4 and CD48 is increased after the removal of sialic acids. 16679516 0 25 part_of thyroglobulin 73:85 arg1 Ser-2730 thyroglobulin Ser-2730 PUBTATOR SpecificSite thyroglobulin 7038 Ser-2730 A single chondroitin 6-sulfate oligosaccharide unit at Ser-2730 of human thyroglobulin enhances hormone formation and limits proteolytic accessibility at the carboxyl terminus. 2498325 4 56 gly glycosylated 570:581 arg1 asialo-apoE asialo-apoE PUBTATOR apoE 348 Sequence analysis and amino sugar analysis of this peptide derived from asialo-, monosialo-, or disialo-apoE indicated that the carbohydrate moiety is attached only to Thr194 in monosialo- and disialo-apoE and that asialo-apoE is not glycosylated. 20837471 6 0 part_of Thr 1105:1107 arg1 ANGPTL3 ANGPTL3 Thr PUBTATOR SpecificSite ANGPTL3 Q9Y5C1 Thr(226) Screening of a panel of proteins known to affect lipid metabolism for potential sites glycosylated by GalNAc-T2 led to identification of Thr(226) adjacent to the proprotein convertase processing site in ANGPTL3. 16763549 3 40 gly NRP1 501:504 arg1 proteoglycan NRP1 proteoglycan OGER NRP1 O14786 Here we show that a substantial fraction of NRP1 is proteoglycan modified with either heparan sulfate or chondroitin sulfate on a single conserved Ser. 3458201 0 77 gly 1B-glycoprotein 42:56 arg1 1B-glycoprotein 1B-glycoprotein PUBTATOR alpha 1B-glycoprotein 1 Amino acid sequence of human plasma alpha 1B-glycoprotein: homology to the immunoglobulin supergene family. 35273390 0 27 gly glycoprotein 39:50 arg1 human glycoprotein 2 human glycoprotein 2 PUBTATOR glycoprotein 2 2813 Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH. 21615908 5 2 gly glycosylated 889:900 arg1 Fibin Fibin PUBTATOR Fibin 67606 Fibin is an evolutionarily conserved protein, carries a cleavable signal peptide (amino acids 1-18) and is glycosylated at Asn30. 3353370 7 45 gly repeats 1490:1496 arg1 the alpha chain alpha chain repeats PUBTATOR alpha chain 2217 The amino-terminal region of the beta chain contains a leucine-rich sequence of 24 amino acids that is similar to a sequence that occurs as seven tandem repeats in the alpha chain of GPIb and nine tandem repeats in leucine-rich alpha 2-glycoprotein. 3353370 7 58 gly repeats 1439:1445 arg1 the alpha chain alpha chain repeats PUBTATOR alpha chain 2217 The amino-terminal region of the beta chain contains a leucine-rich sequence of 24 amino acids that is similar to a sequence that occurs as seven tandem repeats in the alpha chain of GPIb and nine tandem repeats in leucine-rich alpha 2-glycoprotein. 20511397 5 58 part_of Thr 1037:1039 arg1 both cellular and secreted apoE apoE Thr PUBTATOR SpecificSite apoE 348 Thr(194) Our results identify eight different glycoforms with (HexNAc)(2)-Hex(2)-(NeuAc)(2) being the most complex glycan detected on Thr(194) in both cellular and secreted apoE. 11706042 2 29 gly TLR4 355:358 arg1 N-linked carbohydrates TLR4 N-linked carbohydrates PUBTATOR TLR4 7099 Because each of these proteins is glycosylated, we have examined the functional role of N-linked carbohydrates of both MD-2 and TLR4. 11706042 2 32 gly MD-2 346:349 arg1 N-linked carbohydrates MD-2 N-linked carbohydrates PUBTATOR MD-2 23643 Because each of these proteins is glycosylated, we have examined the functional role of N-linked carbohydrates of both MD-2 and TLR4. 8617200 4 2 gly LTBP-1 842:847 arg1 the third 8-Cys repeat LTBP-1 the third 8-Cys repeat PUBTATOR LTBP-1 4052 Immunoblotting of the fusion protein complexes indicated that the third 8-Cys repeat of LTBP-1 bound covalently to the LAP region of TGF-beta1. 22387313 0 42 gly N-glycosylation 0:14 arg1 the mammalian dipeptidyl aminopeptidase-like protein 10 the mammalian dipeptidyl aminopeptidase-like protein 10 PUBTATOR dipeptidyl aminopeptidase-like protein 10 57628 N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. 22387313 0 42 gly N-glycosylation 0:14 arg1 DPP10 DPP10 PUBTATOR DPP10 57628 N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. 1710976 12 12 part_of Peptide 1287:1293 arg1 residues 427-429 Peptide residues 427-429 OGER SpecificSite Peptide residues 427-429 Peptide Asn-Gly-Ser (residues 427-429) is the most probable candidate for glycosylation; literature data suggests that deamidation occurs in the stretch Glu-Asn-Gly-Lys-Asp (residues 364-368) and Asn-Gly-Asn-Cys (residues 474-477). 21763278 6 20 gly carbohydrates 669:681 arg1 pro-BNP BNP carbohydrates PUBTATOR BNP 4879 Glycosidases and glycosylation inhibitors were used to examine carbohydrates on pro-BNP. 19855092 7 18 gly desialylated 1146:1157 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin 9370 Activity of desialylated adiponectin was comparable to control adiponectin in L6 myotubes and acute assays in adiponectin(-/-) mice. 8172892 6 50 gly alpha-D4 993:1000 arg1 Component sugar and sialic acid analyses AP alpha Component sugar and sialic acid analyses PUBTATOR AP alpha 2028 Component sugar and sialic acid analyses of AP alpha-D4 and AP alpha-D5 revealed that they contained 1 mol each of N-acetyl-D-galactosamine (GalNAc), D-galactose (Gal), and sialic acid. 8172892 6 95 gly alpha-D5 1009:1016 arg1 Component sugar and sialic acid analyses AP alpha Component sugar and sialic acid analyses PUBTATOR AP alpha 2028 Component sugar and sialic acid analyses of AP alpha-D4 and AP alpha-D5 revealed that they contained 1 mol each of N-acetyl-D-galactosamine (GalNAc), D-galactose (Gal), and sialic acid. 12889478 0 5 gly L-selectin 57:66 arg1 Endoglycan L-selectin Endoglycan PUBTATOR L-selectin 6402 Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x. 19276170 1 25 gly glycoprotein 240:251 arg1 thrombospondin-5 thrombospondin-5 PUBTATOR thrombospondin-5 1311 Cartilage oligomeric matrix protein (COMP), or thrombospondin-5 (TSP-5), is a secreted glycoprotein that is important for growth plate organization and function. 19276170 1 25 gly glycoprotein 240:251 arg1 Cartilage oligomeric matrix protein Cartilage oligomeric matrix protein OGER Cartilage oligomeric matrix protein P49747 Cartilage oligomeric matrix protein (COMP), or thrombospondin-5 (TSP-5), is a secreted glycoprotein that is important for growth plate organization and function. 8636209 12 50 gly glycosylation 1531:1543 arg1 CD3 delta CD3 delta PUBTATOR CD3 delta 915 Furthermore, the study indicated that, in contrast to CD3 gamma, glycosylation of CD3 delta is required for TCR assembly and expression. 8636209 12 50 gly glycosylation 1531:1543 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 Furthermore, the study indicated that, in contrast to CD3 gamma, glycosylation of CD3 delta is required for TCR assembly and expression. 21712440 7 4 gly glycosylation 1498:1510 arg1 the Aβ1-38/40/42 isoforms the Aβ1-38/40/42 isoforms Cterm Aβ1-38/40/42 isoforms 100034700 We could not detect any glycosylation of the Aβ1-38/40/42 isoforms. 19855092 0 33 gly adiponectin 28:38 arg1 Sialic acid modification adiponectin Sialic acid modification PUBTATOR adiponectin 9370 Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation. 19855092 0 96 gly modification 12:23 arg1 adiponectin AND Sialic acid modification adiponectin Sialic acid modification PUBTATOR adiponectin 9370 Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation. 16201406 4 2 gly found 819:823 arg2 natural hLF AND complex-type glycans natural hLF complex-type glycans PUBTATOR hLF 3131 Even though rhLF contains oligomannose- and hybrid-type N-linked glycans next to complex-type glycans, which are the only glycans found on natural hLF, the structures are identical within the experimental error (r.m.s. deviation of only 0.28 A for the main-chain atoms). 16201406 4 2 gly found 819:823 arg2 natural hLF AND the only glycans natural hLF the only glycans PUBTATOR hLF 3131 Even though rhLF contains oligomannose- and hybrid-type N-linked glycans next to complex-type glycans, which are the only glycans found on natural hLF, the structures are identical within the experimental error (r.m.s. deviation of only 0.28 A for the main-chain atoms). 16201406 4 19 gly contains 706:713 arg1 rhLF AND oligomannose- and hybrid-type N-linked glycans rhLF oligomannose- and hybrid-type N-linked glycans OGER rhLF P02788 Even though rhLF contains oligomannose- and hybrid-type N-linked glycans next to complex-type glycans, which are the only glycans found on natural hLF, the structures are identical within the experimental error (r.m.s. deviation of only 0.28 A for the main-chain atoms). 17139081 0 41 gly glycosylated 37:48 arg1 acid-beta-glucosidase acid-beta-glucosidase PUBTATOR acid-beta-glucosidase 2629 Structural comparison of differently glycosylated forms of acid-beta-glucosidase, the defective enzyme in Gaucher disease. 20457942 2 13 gly glycoprotein 172:183 arg1 RhCG RhCG PUBTATOR RhCG 51458 Human Rh C glycoprotein (RhCG) forms a trimeric complex that plays an essential role in ammonia excretion and renal pH regulation. 20457942 2 13 gly glycoprotein 172:183 arg1 Human Rh C glycoprotein Human Rh C glycoprotein PUBTATOR Human Rh C glycoprotein 51458 Human Rh C glycoprotein (RhCG) forms a trimeric complex that plays an essential role in ammonia excretion and renal pH regulation. 9342320 2 68 gly glycosylated 388:399 arg1 glycosylated human IFN-beta glycosylated human IFN-beta PUBTATOR IFN-beta 3440 To achieve a better understanding of the structural basis for the different activities of alpha and beta IFNs, we have determined the crystal structure of glycosylated human IFN-beta at 2.2-A resolution by molecular replacement. 8216207 0 17 gly site 63:66 arg1 soluble recombinant human thrombomodulin thrombomodulin site PUBTATOR thrombomodulin 7056 Identification of the predominant glycosaminoglycan-attachment site in soluble recombinant human thrombomodulin: potential regulation of functionality by glycosyltransferase competition for serine474. 22412906 9 12 gly N-glycosylation 1400:1414 arg1 the GIP receptor the GIP receptor PUBTATOR GIP receptor 2695 N-glycosylation is also required for expression of the GIP receptor at the plasma membrane and efficient GIP potentiation of glucose-induced insulin secretion from the INS-1 pancreatic beta cell line. 14718370 2 33 part_of subdomains 233:242 arg1 MUC5B MUC5B subdomains PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 33 part_of subdomains 233:242 arg1 MUC5AC MUC5AC subdomains PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 75 part_of Cys1 201:204 arg1 MUC5B MUC5B Cys1 PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 75 part_of Cys1 201:204 arg1 MUC5AC MUC5AC Cys1 PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 80 part_of Cys5 210:213 arg1 MUC5B MUC5B Cys5 PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 80 part_of Cys5 210:213 arg1 MUC5AC MUC5AC Cys5 PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 84 part_of Cys1 219:222 arg1 MUC5B MUC5B Cys1 PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 84 part_of Cys1 219:222 arg1 MUC5AC MUC5AC Cys1 PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 17157876 1 91 gly glycoprotein 208:219 arg1 Human carboxypeptidase N (CPN) Human carboxypeptidase N (CPN) PUBTATOR CPN 1369 Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. 26274980 0 16 part_of G 188:188 arg1 Unconventional Asn71-Glycosylation Neutrophil cathepsin G Unconventional Asn71-Glycosylation PUBTATOR AminoAcid Neutrophil cathepsin G 1511 Asn71 Complementary LC-MS/MS-Based N-Glycan, N-Glycopeptide, and Intact N-Glycoprotein Profiling Reveals Unconventional Asn71-Glycosylation of Human Neutrophil Cathepsin G. Neutrophil cathepsin G (nCG) is a central serine protease in the human innate immune system, but the importance of its N-glycosylation remains largely undescribed. 12878160 7 10 gly VIPL 890:893 arg1 The single N-linked glycan VIPL The single N-linked glycan PUBTATOR VIPL Q9H0V9 The single N-linked glycan of VIPL remained endoglycosidase H-sensitive during a 2-h pulse-chase, even when the protein was overexpressed or mutated to allow export to the plasma membrane. 10756055 0 45 gly glycosylation 9:21 arg1 CXCR4 CXCR4 PUBTATOR CXCR4 7852 N-linked glycosylation of CXCR4 masks coreceptor function for CCR5-dependent human immunodeficiency virus type 1 isolates. 12970363 7 86 gly monoglycosylated 1252:1267 arg1 the monoglycosylated TRPC3 channel the monoglycosylated TRPC3 channel PUBTATOR TRPC3 channel 7222 Immunoblotting analysis of HEK 293 cell lysates expressing TRPC6 wild type and mutants favors a model of TRPC6 that is dually glycosylated within the first (e1) and second extracellular loop (e2) as opposed to the monoglycosylated TRPC3 channel (Vannier, B., Zhu, X., Brown, D., and Birnbaumer, L. (1998) J. Biol. 18420026 0 60 gly glycoprotein 28:39 arg1 Nectin-like molecule 1 Nectin-like molecule 1 PUBTATOR Nectin-like molecule 1 57863 Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity. 27033522 8 36 gly -glycosylation 1136:1149 arg1 the secreted PEBP4 the secreted PEBP4 PUBTATOR PEBP4 157310 Mass spectrometry detected asparagine (N)-glycosylation on the secreted PEBP4. 9767079 1 45 gly N-glycosylation 153:167 arg1 folate carrier folate carrier PUBTATOR reduced folate carrier 6573 The role of N-glycosylation in reduced folate carrier (RFC) transport and membrane targeting was examined in transport-deficient K562 (K500E) cells transfected with human RFC cDNAs. 9767079 1 45 gly N-glycosylation 153:167 arg1 RFC RFC PUBTATOR RFC 6573 The role of N-glycosylation in reduced folate carrier (RFC) transport and membrane targeting was examined in transport-deficient K562 (K500E) cells transfected with human RFC cDNAs. 23010571 5 10 gly glycoprotein 762:773 arg1 Recombinant ADAMTSL5 Recombinant ADAMTSL5 PUBTATOR Recombinant ADAMTSL5 339366 Recombinant ADAMTSL5 is a secreted, N-glycosylated 60kDa glycoprotein located in the subcellular matrix, on the cell-surface, and in the medium of transfected cells. 3571235 2 2 gly sialoglycoproteins 157:174 arg1 glycophorins B and C glycophorins B and C PUBTATOR glycophorins B and C 2994 We have developed methods for the preparative purification of two sialoglycoproteins (glycophorins B and C) from human erythrocyte membranes by high-performance ion exchange and gel permeation chromatography in the presence of Triton X-100. 19119025 1 43 gly glycoprotein 129:140 arg1 Tapasin Tapasin PUBTATOR Tapasin 6892 Tapasin is a glycoprotein critical for loading major histocompatibility complex (MHC) class I molecules with high-affinity peptides. 23723439 4 12 gly glycosylation 998:1010 arg1 α-DG α-DG Cterm DG 1605 We previously reported that human natural killer-1 sulfotransferase (HNK-1ST), which was originally reported as one of the enzymes responsible for HNK-1 glycoepitope, had an ability to suppress the glycosylation and the function of α-DG. 21763278 16 72 part_of Arg-76 1646:1651 arg1 pro-BNP BNP Arg-76 PUBTATOR SpecificSite BNP 4879 Arg-76 In HEK 293 cells, furin cleaved pro-BNP at Arg-76 whereas in cardiomyocytes corin cleaved pro-BNP at multiple residues including Arg-73, Arg-76 and Lys-79. 1472036 4 25 part_of Cys524 925:930 arg1 the other alpha-subunit subunit Cys524 OGER AminoAcid subunit P06213 Cys524 Since it has been shown that the extracellular domain of the insulin receptor has no free thiols and since no other sequences containing cysteine were found in these fractions, we conclude that Cys524 forms a disulfide bond to the Cys524 in the other alpha-subunit. 17157876 8 22 gly subunit 1816:1822 arg1 the central leucine-rich repeat tandem CPN2 subunit the central leucine-rich repeat tandem PUBTATOR CPN2 subunit 1370 In tetrameric CPN, each CPN1 subunit might interact with the central leucine-rich repeat tandem of the cognate CPN2 subunit via a unique hydrophobic surface patch wrapping around the catalytic domain-TT interface, exposing the two active centers. 9572875 7 38 gly nonglycosylated 1296:1310 arg1 nonglycosylated IGFBP-6 nonglycosylated IGFBP-6 PUBTATOR IGFBP-6 3489 Glycosylated IGFBP-6 exhibited greater resistance to proteolysis by chymotrypsin and trypsin than nonglycosylated IGFBP-6. 3056714 2 27 gly glycosylated 366:377 arg1 The CT29-LAP The CT29-LAP PUBTATOR CT29 57082 The CT29-LAP was synthesized in BHK cells as a heterogeneously glycosylated precursor that was tightly membrane associated. 18768590 7 64 gly glycosylated 1189:1200 arg1 The complexly glycosylated TRPV5 The complexly glycosylated TRPV5 OGER TRPV5 Q9NQA5 The complexly glycosylated TRPV5 that appears at the plasma membrane was increased by WNK3. 22750213 4 34 gly glycoprotein 682:693 arg1 PRiMA PRiMA PUBTATOR PRiMA 170952 PRiMA is a glycoprotein containing two putative N-linked glycosylation sites. 1883960 4 18 gly O-glycosylated 1037:1050 arg1 25-kD O-glycosylated IL-6 25-kD O-glycosylated IL-6 PUBTATOR IL-6 3569 Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30, suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site. 1883960 4 89 gly O-glycosylated 1130:1143 arg1 the 28- to 30-kD N- and O-glycosylated IL-6 the 28- to 30-kD N- and O-glycosylated IL-6 PUBTATOR IL-6 3569 Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30, suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site. 18467335 9 27 gly N-glycosylated 997:1010 arg1 PCI PCI OGER PCI P05154 PCI was N-glycosylated at all three potential N-glycosylation sites, Asn-230, Asn-243, and Asn-319, but a small fraction of PCI lacked the N-glycan at Asn-243. 24927598 7 39 part_of N102 1011:1014 arg1 ZIP14 ZIP14 N102 PUBTATOR SpecificSite ZIP14 23516 N102 Asparagine-linked (N-linked) glycosylation of ZIP14, particularly the glycosylation at N102, was required for efficient membrane extraction of ZIP14 and therefore is necessary for its iron sensitivity. 21768335 4 23 gly glycosylated 676:687 arg1 a glycosylated Fcγ receptor a glycosylated Fcγ receptor Cterm Fcγ In this study, the crystal structures of a glycosylated Fcγ receptor complexed with either afucosylated or fucosylated Fc were determined allowing a detailed, molecular understanding of the regulatory role of Fc-oligosaccharide core fucosylation in improving ADCC. 15628971 6 51 gly attached 1280:1287 arg1 C4ST-1 AND N-linked oligosaccharides C4ST-1 N-linked oligosaccharides PUBTATOR C4ST-1 314694 These observations strongly suggest that N-linked oligosaccharides attached to C4ST-1 contribute to the production and stability of the active form of C4ST-1. 18585350 3 38 gly glycoprotein 484:495 arg1 CALHM1 CALHM1 PUBTATOR CALHM1 255022 We show that CALHM1 encodes a multipass transmembrane glycoprotein that controls cytosolic Ca(2+) concentrations and Abeta levels. 18420026 6 20 gly glycoprotein 1539:1550 arg1 human NECL1 human NECL1 PUBTATOR NECL1 57863 Based on the results of N-Glycosidase F treatment with human fetal brain tissue and lysates from transient transfection with human wild-type or glycosylation site mutant NECL1 in 293ET cells, we demonstrated that human NECL1 is an N-linked glycoprotein with a single glycosylation site at position N290KS. 21752865 3 59 gly glycosylated 714:725 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Its apparent molecular mass (42-44 kDa) is significantly higher than that predicted by the number and composition of amino acids (30 kDa) suggesting that BRI2 is glycosylated. 3571235 4 18 part_of B 536:536 arg1 residues 36-71 glycophorin B residues 36-71 PUBTATOR SpecificSite glycophorin B 2994 residues 36-71 The amino acid sequence of the intramembranous domain (residues 36-71) of glycophorin B was determined and found to be similar to that of the hydrophobic region of the major sialoglycoprotein (glycophorin A). 3458201 1 17 gly 1B-glycoprotein 171:185 arg1 alpha 1B-glycoprotein alpha 1B-glycoprotein PUBTATOR alpha 1B-glycoprotein 1 The complete amino acid sequence has been determined for alpha 1B-glycoprotein (alpha 1B), a protein of unknown function present in human plasma. 3458201 1 17 gly 1B-glycoprotein 171:185 arg1 alpha 1B alpha 1B PUBTATOR alpha 1B 1 The complete amino acid sequence has been determined for alpha 1B-glycoprotein (alpha 1B), a protein of unknown function present in human plasma. 26274980 0 78 gly Asn71-Glycosylation 114:132 arg1 nCG nCG Cterm nCG 1511 Complementary LC-MS/MS-Based N-Glycan, N-Glycopeptide, and Intact N-Glycoprotein Profiling Reveals Unconventional Asn71-Glycosylation of Human Neutrophil Cathepsin G. Neutrophil cathepsin G (nCG) is a central serine protease in the human innate immune system, but the importance of its N-glycosylation remains largely undescribed. 26274980 0 78 gly Asn71-Glycosylation 114:132 arg1 Human Neutrophil Cathepsin G. Neutrophil cathepsin G Human Neutrophil Cathepsin G. Neutrophil cathepsin G PUBTATOR Neutrophil cathepsin G 1511 Complementary LC-MS/MS-Based N-Glycan, N-Glycopeptide, and Intact N-Glycoprotein Profiling Reveals Unconventional Asn71-Glycosylation of Human Neutrophil Cathepsin G. Neutrophil cathepsin G (nCG) is a central serine protease in the human innate immune system, but the importance of its N-glycosylation remains largely undescribed. 21768335 3 54 gly afucosylated 471:482 arg1 afucosylated IgGs afucosylated IgGs Cterm IgGs Although afucosylated IgGs exist naturally, a next generation of recombinant therapeutic, glycoenginereed antibodies is currently being developed to exploit this finding. 21752865 0 42 gly Glycosylation 0:12 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Glycosylation of BRI2 on asparagine 170 is involved in its trafficking to the cell surface but not in its processing by furin or ADAM10. 1517205 2 31 gly factor 504:509 arg1 a tetrasaccharide O-fucosidically factor IX a tetrasaccharide O-fucosidically OGER factor IX P00740 We now have evidence of another modification in the first EGF-like domain of human factor IX, which proved to be a tetrasaccharide O-fucosidically linked to Ser-61. 22023369 3 40 gly sFcγRIIIa 568:576 arg1 the two N-glycans FcγRIIIa the two N-glycans PUBTATOR FcγRIIIa 2214 The crystal structure shows that one of the two N-glycans of sFcγRIIIa mediates the interaction with nonfucosylated Fc, thereby stabilizing the complex. 17542669 5 68 part_of 2,500 687:691 arg1 2 large (2,500 A(2) OGER SpecificSite large (2,500 Q8N3Y3 A(2) The interface buried in the complex is large (2,500 A(2)) and an extensive network of ionic, polar, and hydrophobic bonding is involved in the interaction. 1517205 1 13 part_of protein 341:347 arg1 Ser-53 protein Z Ser-53 Cterm SpecificSite protein Z Ser-53 We have recently discovered unusual sugar chains (xylose (Xyl)-glucose (Glc) and (Xyl)2-Glc) linked to a serine residue in the epidermal growth factor (EGF)-like domains of human and bovine clotting factors VII (Ser-52), IX (Ser-53), and protein Z (Ser-53), in addition to bovine platelet glycoprotein thrombospondin. 17286803 4 19 gly glycosylated 519:530 arg1 CLN3 CLN3 OGER CLN3 Q13286 Mutational analysis revealed that in COS7 cells, CLN3 is glycosylated at asparagine residues 71 and 85. 17711303 12 66 gly N-glycosylation 1808:1822 arg1 the hKOR the hKOR PUBTATOR hKOR 4986 Thus, N-glycosylation of the hKOR plays important roles in stability and trafficking along the biosynthesis pathway of the receptor protein as well as agonist-induced receptor regulation. 12970363 5 58 gly glycosylation 842:854 arg1 TRPC6 TRPC6 PUBTATOR TRPC6 7225 To identify potential molecular correlates accounting for the functional difference, we analyzed the glycosylation pattern of TRPC6 compared with TRPC3. 23723439 5 48 gly glycosylation 1095:1107 arg1 α-DG α-DG Cterm DG 1605 In this study, we investigated how HNK-1ST regulates the glycosylation of α-DG using deletion and mutation analyses. 3881423 7 42 gly glycoproteins 1259:1271 arg1 neutral alpha-glucosidase AB neutral alpha-glucosidase AB OGER neutral alpha-glucosidase AB Q14697 Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 3881423 7 42 gly glycoproteins 1259:1271 arg1 glucosidase II glucosidase II OGER glucosidase II Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 3881423 7 48 gly have 1341:1344 arg1 neutral alpha-glucosidase AB AND the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside neutral alpha-glucosidase AB the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside OGER neutral alpha-glucosidase AB Q14697 Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 3881423 7 48 gly have 1341:1344 arg1 glucosidase II AND the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside glucosidase II the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside OGER glucosidase II Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 9233787 1 9 gly glycoprotein 166:177 arg1 BM-40 BM-40 OGER BM-40 P09486 BM-40 (also known as SPARC or osteonectin) is an anti-adhesive secreted glycoprotein involved in tissue remodelling. 2013294 1 53 gly glycoprotein 118:129 arg1 Secretory actin-binding protein Secretory actin-binding protein PUBTATOR Secretory actin-binding protein 5304 Secretory actin-binding protein (SABP), a glycoprotein from human seminal plasma, was isolated according to Akiyama and Kimura [Akiyama, K. & Kimura, H. (1990) Biochim. 10531415 0 46 gly glycosylation 9:21 arg1 dopamine receptors D1, dopamine receptors PUBTATOR D1, dopamine receptors 25802 N-linked glycosylation is required for plasma membrane localization of D5, but not D1, dopamine receptors in transfected mammalian cells. 23527852 1 10 gly glycoprotein 175:186 arg1 Apolipoprotein-CIII Apolipoprotein-CIII PUBTATOR Apolipoprotein-CIII 345 Apolipoprotein-CIII (apoCIII) is an abundant blood glycoprotein associated with lipoprotein particles. 19855092 9 97 gly desialylated 1492:1503 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin 11450 Uptake of desialylated adiponectin by isolated primary rat hepatocytes was also accelerated, suggesting a role for the hepatic asialoglycoprotein receptor. 9578468 0 41 gly O-glycosylated 42:55 arg1 human eotaxin human eotaxin PUBTATOR eotaxin 6356 Delayed production of biologically active O-glycosylated forms of human eotaxin by tumor-necrosis-factor-alpha-stimulated dermal fibroblasts. 17018531 2 28 gly glycoprotein 340:351 arg1 NPC2 NPC2 PUBTATOR NPC2 10577 One form of the disease is caused by a deficiency in NPC2, a soluble lysosomal glycoprotein that binds cholesterol. 22688517 2 57 gly N-glycosylation 216:230 arg1 LOX-1 LOX-1 PUBTATOR LOX-1 4973 The N-glycosylation of LOX-1 has been shown to affect its biological functions in vivo and modulate the pathogenesis of atherosclerosis. 21138434 2 77 gly N-glycosylation 373:387 arg1 the 5-HT3A subunit the 5-HT3A subunit PUBTATOR 5-HT3A subunit 3359 Since N-glycosylation of the 5-HT3A subunit impacts cell surface trafficking, the presence of N-glycosylation of the human (h) 5-HT3B subunit and the influence upon cell membrane expression was investigated. 21138434 2 83 gly N-glycosylation 461:475 arg1 the human (h) 5-HT3B subunit the human (h) 5-HT3B subunit PUBTATOR 5-HT3B subunit 9177 Since N-glycosylation of the 5-HT3A subunit impacts cell surface trafficking, the presence of N-glycosylation of the human (h) 5-HT3B subunit and the influence upon cell membrane expression was investigated. 15628971 4 86 gly attached 489:496 arg1 C4ST-1 AND the N-linked oligosaccharides C4ST-1 the N-linked oligosaccharides PUBTATOR C4ST-1 314694 In the present paper, we investigated the functional role of the N-linked oligosaccharides attached to C4ST-1. 8670172 5 88 part_of Asn-77 888:893 arg1 a GPI-anchor GPI Asn-77 OGER SpecificSite GPI P06744 Asn-77 Mass analysis of the isolated C-terminal peptide (T9) indicated that a GPI-anchor (at Asn-77) without an inositol-associated phospholipid was present in soluble CD59u. 12122212 7 38 gly glycosylation 1154:1166 arg1 CD21 CD21 Cterm CD21 1380 We present evidence that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding. 9153399 8 45 gly ICAM-2 1268:1273 arg1 N-linked glycans ICAM-2 N-linked glycans PUBTATOR ICAM-2 3384 A bend between domains 1 and 2 of ICAM-2 and a tripod-like arrangement of N-linked glycans in the membrane-proximal region of domain 2 may be important for presenting the recognition surface to LFA-1. 28668641 0 25 gly Glycosylation 0:12 arg1 SERPINA12 SERPINA12 PUBTATOR SERPINA12 145264 Glycosylation of human vaspin (SERPINA12) and its impact on serpin activity, heparin binding and thermal stability. 28668641 0 25 gly Glycosylation 0:12 arg1 human vaspin human vaspin PUBTATOR vaspin 145264 Glycosylation of human vaspin (SERPINA12) and its impact on serpin activity, heparin binding and thermal stability. 8172892 4 82 part_of AP 645:646 arg1 residues 157-165 AP alpha residues 157-165 PUBTATOR SpecificSite AP alpha 2028 residues 157-165 A nonapeptide (AP alpha-D4, residues 157-165) and an undecapeptide (AP alpha-D5, 166-176) derived from AP alpha contained Thr-159 and Thr-169, neither of which could be identified using a gas-phase protein sequencer. 8172892 4 71 part_of contained 742:750 arg1 AP alpha-D5, 166-176 AND Thr-159 AP alpha Thr-159 and Thr-169 PUBTATOR SpecificSite AP alpha 2028 Thr-159 and Thr-169 A nonapeptide (AP alpha-D4, residues 157-165) and an undecapeptide (AP alpha-D5, 166-176) derived from AP alpha contained Thr-159 and Thr-169, neither of which could be identified using a gas-phase protein sequencer. 22363519 4 22 gly Toll-like 582:590 arg1 a model repeat protein Toll-like receptor4 a model repeat protein PUBTATOR Toll-like receptor4 7099 As a model repeat protein, a Toll-like receptor4 (TLR4) decoy receptor composed of leucine-rich repeat (LRR) modules was used, and its interaction interface was rationally engineered to increase the binding affinity for myeloid differentiation protein 2 (MD2). 22363519 4 71 gly TLR4 603:606 arg1 a model repeat protein TLR4 a model repeat protein PUBTATOR TLR4 7099 As a model repeat protein, a Toll-like receptor4 (TLR4) decoy receptor composed of leucine-rich repeat (LRR) modules was used, and its interaction interface was rationally engineered to increase the binding affinity for myeloid differentiation protein 2 (MD2). 22363519 4 80 gly receptor4 592:600 arg1 a model repeat protein Toll-like receptor4 a model repeat protein PUBTATOR Toll-like receptor4 7099 As a model repeat protein, a Toll-like receptor4 (TLR4) decoy receptor composed of leucine-rich repeat (LRR) modules was used, and its interaction interface was rationally engineered to increase the binding affinity for myeloid differentiation protein 2 (MD2). 22601780 3 6 gly contains 344:351 arg1 Del-1 AND 3 epidermal growth factor (EGF)-like repeats Del-1 3 epidermal growth factor (EGF)-like repeats PUBTATOR Del-1 10085 Del-1 contains 3 epidermal growth factor (EGF)-like repeats and 2 discoidin-like domains. 22750213 5 14 gly glycosylation 837:849 arg1 PRiMA PRiMA PUBTATOR PRiMA 170952 By using site-directed mutagenesis, the asparagine-43 was identified to be the N-linked glycosylation site of PRiMA. 8670172 14 8 gly CD59 1926:1929 arg1 N-linked oligosaccharide CD59 N-linked oligosaccharide OGER CD59 P13987 These results document the structural heterogeneity of both the GPI anchor and N-linked oligosaccharide of CD59 and demonstrate that the phospholipid tail is needed for the full functional activity of CD59. 21138434 6 23 gly N-glycosylation 1402:1416 arg1 the h5-HT3B subunit the h5-HT3B subunit Cterm h5-HT3B subunit 9177 The present study has identified utilised N-glycosylation sites of the h5-HT3B subunit and demonstrated that they promote subunit expression in the cell membrane; a prerequisite for 5-HT(3) receptor function. 12391027 1 35 gly TGFbeta 263:269 arg1 glycosaminoglycan binding TGFbeta glycosaminoglycan binding PUBTATOR TGFbeta 7040 Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. 12391027 1 14 gly contains 125:132 arg1 Thrombospondin-1 AND three type 1 repeats Thrombospondin-1 three type 1 repeats PUBTATOR Thrombospondin-1 7057 Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. 12391027 1 14 gly contains 125:132 arg1 TSP-1 AND three type 1 repeats TSP-1 three type 1 repeats PUBTATOR TSP-1 7057 Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. 12408961 6 41 gly N-glycosylated 816:829 arg1 Human and mouse CREG2 Human and mouse CREG2 PUBTATOR CREG2 263764 Human and mouse CREG2 are N-glycosylated in HeLa cells and deletion of amino-terminal sequences completely abolishes N-glycosylation, indicating that the N termini of both proteins may function as signal sequences. 8617200 0 29 gly LTBP-1 117:122 arg1 an eight cysteine repeat LTBP-1 an eight cysteine repeat PUBTATOR LTBP-1 4052 Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1. 20823119 0 83 gly IgA1 23:26 arg1 O-glycans IgA1 O-glycans PUBTATOR IgA1 P01876 Clustered O-glycans of IgA1: defining macro- and microheterogeneity by use of electron capture/transfer dissociation. 25760607 6 38 gly glycosylated 750:761 arg1 glycosylated LLT1 glycosylated LLT1 PUBTATOR LLT1 29121 The hexamer of glycosylated LLT1 consists of three classical dimers. 26811476 7 0 gly glycoprotein 1322:1333 arg1 glycoprotein 2 glycoprotein 2 PUBTATOR glycoprotein 2 2813 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 26811476 7 0 gly glycoprotein 1322:1333 arg1 GP2 GP2 PUBTATOR GP2 2813 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 26811476 7 52 gly glycoproteins 1285:1297 arg1 glycoprotein 2 glycoprotein 2 PUBTATOR glycoprotein 2 2813 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 26811476 7 52 gly glycoproteins 1285:1297 arg1 α-tectorin α-tectorin PUBTATOR -tectorin 7007 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 20511397 3 77 gly structures 590:599 arg1 plasma apoE apoE structures PUBTATOR apoE 348 Some of the glycan structures on plasma apoE are characterized; however, the more complicated structures on plasma and cellular/secreted apoE remain unidentified. 9524075 9 36 gly non-glycosylated 1048:1063 arg1 the glycosylated and non-glycosylated procathepsin S the glycosylated and non-glycosylated procathepsin S Cterm the glycosylated and non-glycosylated procathepsin S 1520 A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. 9524075 9 55 gly glycosylated 1031:1042 arg1 the glycosylated and non-glycosylated procathepsin S the glycosylated and non-glycosylated procathepsin S Cterm the glycosylated and non-glycosylated procathepsin S 1520 A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. 15477100 0 64 gly Zn-alpha2-glycoprotein 46:67 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein. 31310764 1 24 gly glycoprotein 103:114 arg1 The mucin 2 glycoprotein The mucin 2 glycoprotein PUBTATOR mucin 2 glycoprotein Q02817 The mucin 2 glycoprotein assembles into a complex hydrogel that protects intestinal epithelia and houses the gut microbiome. 12144777 1 21 gly glycoprotein 125:136 arg1 Human renal dipeptidase Human renal dipeptidase PUBTATOR renal dipeptidase 1800 Human renal dipeptidase is a membrane-bound glycoprotein hydrolyzing dipeptides and is involved in hydrolytic metabolism of penem and carbapenem beta-lactam antibiotics. 20357243 1 65 gly G 235:235 arg1 glycans human immunoglobulin G glycans Cterm human immunoglobulin G EndoS from Streptococcus pyogenes is an immunomodulating enzyme that specifically hydrolyzes glycans from human immunoglobulin G and thereby affects antibody effector functions. 21752865 5 65 gly N-glycosylated 1011:1024 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Given that N-glycosylation is considered essential for protein folding, processing and trafficking, we examined whether BRI2 is N-glycosylated. 8576151 5 63 gly glycosylated 636:647 arg1 The purified procollagenase-3 The purified procollagenase-3 PUBTATOR collagenase-3 4322 The purified procollagenase-3 was shown to be glycosylated and displayed a M(r) of 60,000, the N-terminal sequence being LPLPSGGD, which is consistent with the cDNA-predicted sequence. 2498325 9 15 gly N-glycosylated 1435:1448 arg1 this apoE this apoE PUBTATOR apoE P02649 Studies with tunicamycin indicated that this apoE was N-glycosylated at Asn194. 19358553 3 64 gly ribonuclease 645:656 arg1 neutral glycans ribonuclease B neutral glycans Cterm ribonuclease B Glycopeptides derived from glycoproteins containing neutral glycans (ribonuclease B, IgG, and ovalbumin) were initially profiled and yielded excellent and reproducible quantitation (correlation coefficient r = 0.9958, n = 5) when evaluated against a normal phase HPLC 2-AB glycan profile. 12775711 1 37 gly glycoprotein 140:151 arg1 HCGP39 HCGP39 PUBTATOR HCGP39 1116 The 39-kDa human cartilage glycoprotein (HCGP39), a member of a novel family of chitinase-like lectins (Chilectins), is overexpressed in articular chondrocytes and certain cancers. 21886772 8 18 gly N-glycosylation 1415:1429 arg1 FKRP homodimer FKRP homodimer PUBTATOR FKRP homodimer 79147 FKRP contains N-glycan of high mannose and/or hybrid type; however, FKRP N-glycosylation is not required for FKRP homodimer or multimer formation. 21886772 8 22 gly contains 1347:1354 arg1 FKRP AND N-glycan FKRP N-glycan PUBTATOR FKRP 79147 FKRP contains N-glycan of high mannose and/or hybrid type; however, FKRP N-glycosylation is not required for FKRP homodimer or multimer formation. 21752865 8 19 gly N-glycosylation 1296:1310 arg1 BRI2 trafficking BRI2 trafficking PUBTATOR BRI2 9445 To examine the effect of N-glycosylation on BRI2 trafficking at the cell surface, we performed biotinylation and (35)S methionine pulse-chase experiments. 14699159 4 74 gly glycosylation 968:980 arg1 newly synthesized p90ATF6 newly synthesized p90ATF6 Cterm p90ATF6 22926 Here we show that ER Ca(2+) depletion stress, a triggering mechanism for the UPR, induces the formation of ATF6(f), which represents de novo partial glycosylation of newly synthesized p90ATF6. 15014436 9 43 gly TSP-5/COMP 1091:1100 arg1 the T3 repeats TSP the T3 repeats PUBTATOR TSP 1311 Mutations in the T3 repeats of TSP-5/COMP, which cause two human skeletal disorders, are predicted to disrupt the tertiary structure of the T3-CTD assembly. 10209036 3 10 gly glycoprotein 482:493 arg1 SIT SIT PUBTATOR SIT 27240 SIT is a disulfide-linked homodimeric glycoprotein that is expressed in lymphocytes. 8636209 7 68 gly glycosylation 864:876 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 Mutagenesis of N-linked glycosylation sites showed that glycosylation of CD3 gamma is not required for TCR assembly and expression. 8636209 7 68 gly glycosylation 864:876 arg1 CD3 gamma CD3 gamma PUBTATOR CD3 gamma 917 Mutagenesis of N-linked glycosylation sites showed that glycosylation of CD3 gamma is not required for TCR assembly and expression. 8962717 0 56 gly glycosylation 16:28 arg1 the epidermal growth factor receptor the epidermal growth factor receptor PUBTATOR epidermal growth factor receptor P00533 Analysis of the glycosylation patterns of the extracellular domain of the epidermal growth factor receptor expressed in Chinese hamster ovary fibroblasts. 17715132 3 18 gly N-glycosylated 480:493 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We show here that Pannexin1 forms a hexameric channel and reaches the cell surface but, unlike connexins, is N-glycosylated. 3264725 8 62 gly glycosylated 1584:1595 arg1 the recombinant factor VIIa the recombinant factor VIIa Cterm factor VIIa In the recombinant factor VIIa, asparagine residue 322 was fully glycosylated whereas asparagine residue 145 was only partially (approximately 66%) glycosylated. 28668641 2 23 gly glycosylation 395:407 arg1 human vaspin human vaspin PUBTATOR vaspin 145264 In this study, we have investigated the glycosylation of human vaspin and its effects on biochemical properties relevant to vaspin function. 12526797 2 13 part_of ICAM-1 366:371 arg1 Glu-34 ICAM-1 Glu-34 PUBTATOR SpecificSite ICAM-1 3383 Glu-34 The I domain Mg2+ directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain residue Glu-241 enables a critical salt bridge. 25922362 6 45 gly desialylated 1481:1492 arg1 EGFR EGFR PUBTATOR EGFR 1956 Moreover, through western blots and mass spectrometry analysis, we found that EGFR immunoprecipitated from cells overexpressing active NEU3, unlike the receptor from mock cells and cells overexpressing inactive NEU3, is desialylated. 17395589 0 38 gly O-fucosylation 0:13 arg1 ADAMTS13 secretion ADAMTS13 secretion PUBTATOR ADAMTS13 11093 O-fucosylation is required for ADAMTS13 secretion. 1533633 8 61 gly unglycosylated 1173:1186 arg1 The unglycosylated alpha-subunit The unglycosylated alpha-subunit OGER subunit P06865 The unglycosylated alpha-subunit, resulting from genetic alteration of all three glycosylation sites or synthesis of the wild-type protein in the presence of tunicamycin, was catalytically inactive. 3264725 7 19 gly N-glycosylated 1475:1488 arg1 human plasma factor VIIa human plasma factor VIIa Cterm factor VIIa Asparagine residues 145 and 322 were found to be fully N-glycosylated in human plasma factor VIIa. 9524075 11 66 gly non-glycosylated 1245:1260 arg1 non-glycosylated procathepsin S non-glycosylated procathepsin S Cterm non-glycosylated procathepsin S 1520 Subcellular fractionation showed non-glycosylated procathepsin S in the membrane fraction. 12022871 8 4 gly glycosylated 1241:1252 arg1 Human TGH Human TGH PUBTATOR Human TGH 1066 Human TGH was glycosylated in the insect cells. 22688517 3 77 gly N-glycosylation 362:376 arg1 LOX-1 LOX-1 PUBTATOR LOX-1 4973 However, the N-glycosylation pattern of LOX-1 has not been described yet. 1569071 9 16 gly unglycosylated 1800:1813 arg1 precursor IGF-II precursor IGF-II PUBTATOR IGF-II P01344 This was the Mr value that would be predicted for an unglycosylated form of precursor IGF-II that had a carboxyl-terminal end at or near Lys88. 3202829 8 27 gly glycosylation 1074:1086 arg1 human seminal RNase human seminal RNase OGER RNase P07998 The glycosylation pattern of human seminal RNase is very similar to that of the pancreatic enzyme. 2498325 7 85 gly glycosylation 1122:1134 arg1 apoE apoE PUBTATOR apoE P02649 Apolipoprotein E(Thr194----Ala) was secreted exclusively as the asialo isoform, confirming that Thr194 is the site of carbohydrate attachment in these cells and indicating that glycosylation of apoE is not essential for secretion. 22688517 5 67 gly LOX-1 995:999 arg1 N-glycans structures LOX-1 N-glycans structures PUBTATOR LOX-1 4973 Here, an approach using nonspecific protease (Pronase E) digestion followed by MALDI-QIT-TOF MS and multistage MS (MS(3)) analysis is explored to obtain site-specific N-glycosylation information of recombinant human LOX-1, in combination with glycan structure confirmation through characterizing released glycans using tandem MS. The results reveal that N-glycans structures as well as their corresponding attached site of LOX-1 can be identified simultaneously by direct MS analysis of glycopeptides from non-specific protease digestion. 23723439 2 0 gly attached 420:427 arg1 its extracellular α-DG subunit AND O-mannosyl glycans its extracellular α-DG subunit O-mannosyl glycans Cterm DG subunit 1605 Since the ligand-binding activity of DG strictly depends on O-mannosyl glycans attached to its extracellular α-DG subunit, aberrant glycosylation causes dystroglycanopathy, a subclass of congenital muscular dystrophy. 2243102 6 15 part_of Asn-34 953:958 arg1 lamp-1 lamp-1 Asn-34 PUBTATOR SpecificSite lamp-1 3916 Asn-34, Asn-93 Amino acid analysis and sequencing demonstrated that polylactosaminoglycans were located at Asn-34, Asn-93 and/or Asn-102, and Asn-195 and/or Asn-200 in lamp-1, and at Asn-4 and/or Asn-10, and Asn-279 in lamp-2. 2243102 2 26 gly glycoproteins 145:157 arg1 lamp-1 lamp-1 PUBTATOR lamp-1 3916 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 2243102 2 26 gly glycoproteins 145:157 arg1 lamp-2 lamp-2 PUBTATOR lamp-2 3920 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 2243102 2 29 gly contain 198:204 arg1 lamp-1 AND 18 and 16 N-glycans lamp-1 18 and 16 N-glycans PUBTATOR lamp-1 3916 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 2243102 2 29 gly contain 198:204 arg1 lamp-2 AND 18 and 16 N-glycans lamp-2 18 and 16 N-glycans PUBTATOR lamp-2 3920 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 21757827 5 11 gly O-glycosylated 776:789 arg1 YIPF3 YIPF3 PUBTATOR YIPF3 25844 Biochemical and immunofluorescence experiments strongly indicated that YIPF3 is synthesized in the ER as a N-glycosylated form (40 kDa), is then O-glycosylated in the Golgi apparatus to become a lower mobility form (46 kDa) and finally becomes a higher mobility form cleaved at its C-terminal luminal domain (36 kDa). 21757827 5 41 gly N-glycosylated 738:751 arg1 YIPF3 YIPF3 PUBTATOR YIPF3 25844 Biochemical and immunofluorescence experiments strongly indicated that YIPF3 is synthesized in the ER as a N-glycosylated form (40 kDa), is then O-glycosylated in the Golgi apparatus to become a lower mobility form (46 kDa) and finally becomes a higher mobility form cleaved at its C-terminal luminal domain (36 kDa). 17924658 0 75 gly glycosylation 65:77 arg1 recombinant human gamma-glutamyltranspeptidase recombinant human gamma-glutamyltranspeptidase PUBTATOR gamma-glutamyltranspeptidase 102724197 Kinetic characterization and identification of the acylation and glycosylation sites of recombinant human gamma-glutamyltranspeptidase. 1533633 0 64 gly glycosylation 16:28 arg1 the alpha-subunit the alpha-subunit OGER subunit P06865 Analysis of the glycosylation and phosphorylation of the alpha-subunit of the lysosomal enzyme, beta-hexosaminidase A, by site-directed mutagenesis. 26274980 6 63 part_of nCG 1749:1751 arg1 nCG Asn71-glycosylation nCG nCG Asn71-glycosylation Cterm AminoAcid nCG 1511 Asn71 Importantly, this study now facilitates investigation of the functional role of nCG Asn71-glycosylation. 23050552 8 46 gly fibrinogen 1086:1095 arg1 The previously reported N-glycan attachment sites fibrinogen The previously reported N-glycan attachment sites PUBTATOR fibrinogen 2244 The previously reported N-glycan attachment sites of human fibrinogen could be confirmed. 21606496 4 7 gly glycosylation 543:555 arg1 2B4 2B4 OGER 2B4 Q9BZW8 Using a recombinant fusion protein of the extracellular domain of 2B4, we demonstrate that N-linked glycosylation of 2B4 is essential for the binding to its ligand CD48. 16679516 10 90 gly hTg-CS 1570:1575 arg1 the chondroitin 6-sulfate oligosaccharide unit hTg the chondroitin 6-sulfate oligosaccharide unit OGER hTg P01266 Furthermore, the chondroitin 6-sulfate oligosaccharide unit of hTg-CS protected peptide bond Lys2714-Gly2715 from proteolysis, during the limited digestion of hTg-CS with trypsin. 2737288 0 9 gly glycosylation 41:53 arg1 human pancreatic elastase 1 human pancreatic elastase 1 PUBTATOR pancreatic elastase 1 1990 Localization and characterization of the glycosylation site of human pancreatic elastase 1. 28165004 3 24 gly pembrolizumab/PD-1 418:435 arg1 the complex structure PD-1 the complex structure PUBTATOR PD-1 5133 However, the binding mechanism of nivolumab to PD-1 has not yet been shown, despite a recent report describing the complex structure of pembrolizumab/PD-1. 19508227 0 14 gly Glycosylation 0:12 arg1 tetraspanin Tspan-1 tetraspanin Tspan-1 OGER Tspan-1 O60635 Glycosylation of tetraspanin Tspan-1 at four distinct sites promotes its transition through the endoplasmic reticulum. 20581009 0 43 gly Endocan 85:91 arg1 a soluble endothelial proteoglycan Endocan a soluble endothelial proteoglycan PUBTATOR Endocan 11082 Characterization and binding activity of the chondroitin/dermatan sulfate chain from Endocan, a soluble endothelial proteoglycan. 19285951 1 37 gly glycoprotein 128:139 arg1 HRG HRG PUBTATOR HRG 3273 Histidine-rich glycoprotein (HRG) is a plasma protein implicated in the innate immune system. 15687489 7 42 gly glycosylated 911:922 arg1 the glycosylated human pFGE the glycosylated human pFGE PUBTATOR pFGE 25870 We have crystallized the glycosylated human pFGE and determined its crystal structure at a resolution of 1.86 A. 21569239 0 76 gly glycosylated 35:46 arg1 F-spondin F-spondin PUBTATOR F-spondin 10418 The structure of the Ca²+-binding, glycosylated F-spondin domain of F-spondin - A C2-domain variant in an extracellular matrix protein. 9767079 3 50 gly glycosylated 545:556 arg1 glycosylated RFC glycosylated RFC PUBTATOR RFC 6573 At 3 microg/ml tunicamycin, the nearly complete loss of glycosylated RFC was accompanied by a approximately 25% decreased rate of methotrexate uptake. 10756055 3 31 gly glycoprotein 736:747 arg1 Env Env PUBTATOR Env 100616444 We hypothesize that this alteration unmasks existing common extracellular structures reflecting a conserved three-dimensional similarity of important elements of CXCR4 and CCR5 that are involved in HIV envelope glycoprotein (Env) interaction. 10756055 3 31 gly glycoprotein 736:747 arg1 HIV envelope glycoprotein HIV envelope glycoprotein PUBTATOR HIV envelope glycoprotein 100616444 We hypothesize that this alteration unmasks existing common extracellular structures reflecting a conserved three-dimensional similarity of important elements of CXCR4 and CCR5 that are involved in HIV envelope glycoprotein (Env) interaction. 18491227 9 20 gly N-glycosylation 1457:1471 arg1 E-cadherin expression E-cadherin expression PUBTATOR E-cadherin P12830 In conclusion, this study revealed that N-glycosylation at Asn-633 is essential for E-cadherin expression, folding and trafficking. 19571171 1 55 gly Golgi-resident 152:165 arg1 N-Acetylglucosamine-6-sulfotransferase-1 Golgi-resident N-Acetylglucosamine-6-sulfotransferase-1 Cterm Golgi-resident N-Acetylglucosamine-6-sulfotransferase-1 (GlcNAc6ST-1) is a Golgi-resident glycoprotein that is responsible for sulfation of the l-selectin ligand on endothelial cells. 22750213 6 56 gly glycosylation 877:889 arg1 mouse PRiMA mouse PRiMA PUBTATOR PRiMA 170952 Abolishing glycosylation on mouse PRiMA appeared not to affect its assembly with AChE(T), the enzymatic properties of AChE, and the membrane trafficking of PRiMA-linked AChE tetramers. 18703501 7 25 part_of contains 1209:1216 arg1 CA IX AND Asn(309) CA IX Asn(309) PUBTATOR SpecificSite CA IX 768 Asn(309) Mass spectrometry experiments showed that CA IX contains an intramolecular disulfide bridge (Cys(119)-Cys(299)) and a unique N-linked glycosylation site (Asn(309)) that bears high mannose-type glycan structures. 7574684 1 20 gly glycoprotein 241:252 arg1 a human neurotrophin receptor trkB a human neurotrophin receptor trkB PUBTATOR trkB 4915 An extracellular domain of a human neurotrophin receptor trkB was expressed in Chinese hamster ovary cells and isolated as a glycoprotein possessing binding activity for brain-derived neurotrophic factor. 15809306 7 59 part_of Ala 1037:1039 arg1 FAPalpha FAPalpha Ala PUBTATOR SpecificSite FAPalpha 2191 Ala(657) Ala(657) in FAPalpha, instead of Asp(663) as in DP-PIV, reduces the acidity in this pocket, and this change could explain the lower affinity for N-terminal amines by FAPalpha. 12889478 4 44 gly L-selectin 821:830 arg1 endoglycan L-selectin endoglycan PUBTATOR L-selectin 6402 We report here that endoglycan, like the two other members of this family (CD34 and podocalyxin) can function as a L-selectin ligand. 9030779 2 63 gly sphingomyelinase 379:394 arg1 the six potential N-linked oligosaccharide chains acid sphingomyelinase the six potential N-linked oligosaccharide chains PUBTATOR acid sphingomyelinase 6609 We have determined the influence of the six potential N-linked oligosaccharide chains of human acid sphingomyelinase (ASM) on catalytic activity, targeting, and processing of the enzyme. 21752865 4 61 gly N-glycosylated 857:870 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 In support, bioinformatics analysis indicated that BRI2 bears the consensus sequence Asn-Thr-Ser (residues 170-173) and could be N-glycosylated at Asn170. 1694179 0 45 gly glycosylated 22:33 arg1 Beta protein C Beta protein C OGER protein C P02810 Beta protein C is not glycosylated at asparagine 329. 18467335 4 15 gly heterogeneity 486:498 arg1 blood-derived PCI blood-derived PCI OGER PCI P05154 In this study we have for the first time provided a full explanation for the marked size heterogeneity of blood-derived PCI and identified functional differences between naturally occurring PCI variants. 21763278 3 31 gly glycosylation 355:367 arg1 pro-BNP pro-BNP PUBTATOR AminoAcid BNP 4879 In this study, we analyzed glycosylation and proteolytic processing of pro-BNP in cardiomyocytes. 11279095 2 1 gly Polysialylation 190:204 arg1 NCAM NCAM PUBTATOR NCAM 4684 Polysialylation of the neural cell adhesion molecule (NCAM) is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which contain sialylmotifs L and S conserved in all members of the sialyltransferases. 11279095 2 1 gly Polysialylation 190:204 arg1 the neural cell adhesion molecule the neural cell adhesion molecule PUBTATOR neural cell adhesion molecule 4684 Polysialylation of the neural cell adhesion molecule (NCAM) is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which contain sialylmotifs L and S conserved in all members of the sialyltransferases. 11279095 2 52 gly molecule 234:241 arg1 Polysialylation neural cell adhesion molecule Polysialylation PUBTATOR neural cell adhesion molecule 4684 Polysialylation of the neural cell adhesion molecule (NCAM) is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which contain sialylmotifs L and S conserved in all members of the sialyltransferases. 6203908 7 70 part_of subunit 660:666 arg1 Cys-447 subunit Cys-447 OGER SpecificSite subunit P01023 Cys-447 Cys-447 probably forms an interchain bridge with Cys-447 from another subunit. 21733844 7 45 gly glycosylated 988:999 arg1 glycosylated hAQP10 glycosylated hAQP10 PUBTATOR hAQP10 89872 In contrast, glycosylated hAQP10 showed increased thermostability of 3-6 °C compared with the nonglycosylated protein, suggesting a stabilizing effect of the N-linked glycan. 24692546 4 1 gly deglycosylated 650:663 arg1 deglycosylated FSH deglycosylated FSH OGER FSH It also predicts that, upon dissociation of the FSHR trimer into monomers, the binding of glycosylated FSH, but not deglycosylated FSH, would increase 3-fold, and that the dissociated monomers would in turn enhance FSHR binding and signaling activities by 3-fold. 24692546 4 29 gly glycosylated 624:635 arg1 glycosylated FSH glycosylated FSH OGER FSH It also predicts that, upon dissociation of the FSHR trimer into monomers, the binding of glycosylated FSH, but not deglycosylated FSH, would increase 3-fold, and that the dissociated monomers would in turn enhance FSHR binding and signaling activities by 3-fold. 20581009 3 31 gly Endocan 567:573 arg1 the glycosaminoglycan (GAG) chain Endocan the glycosaminoglycan (GAG) chain PUBTATOR Endocan 11082 In this work, we characterized the glycosaminoglycan (GAG) chain of Endocan, purified either from the naturally producing human umbilical vein endothelial cells (HUVEC) or from a recombinant over-expression system in human embryonic kidney cells (HEK). 12889478 1 32 gly L-selectin 333:342 arg1 several carbohydrate-modified ligands L-selectin several carbohydrate-modified ligands PUBTATOR L-selectin 6402 During lymphocyte homing to secondary lymphoid organs and instances of inflammatory trafficking, the rolling of leukocytes on vascular endothelium is mediated by transient interactions between L-selectin on leukocytes and several carbohydrate-modified ligands on the endothelium. 10206894 1 11 gly Zn-alpha2-glycoprotein 81:102 arg1 ZAG ZAG PUBTATOR ZAG 563 Zn-alpha2-glycoprotein (ZAG) is a soluble protein that is present in serum and other body fluids. 10206894 1 11 gly Zn-alpha2-glycoprotein 81:102 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Zn-alpha2-glycoprotein (ZAG) is a soluble protein that is present in serum and other body fluids. 21733844 8 49 gly glycosylated 1187:1198 arg1 hAQP10 hAQP10 PUBTATOR hAQP10 89872 Because only one third of hAQP10 was glycosylated yet the thermostability titration was mono-modal, we suggest that the presence of at least one glycosylated protein within each tetramer is sufficient to convey an enhanced structural stability to the remaining hAQP10 protomers of the tetramer. 22750213 0 45 gly glycosylation 9:21 arg1 globular tetrameric acetylcholinesterase globular tetrameric acetylcholinesterase PUBTATOR acetylcholinesterase 11423 N-linked glycosylation of proline-rich membrane anchor (PRiMA) is not required for assembly and trafficking of globular tetrameric acetylcholinesterase. 22750213 0 45 gly glycosylation 9:21 arg1 PRiMA PRiMA PUBTATOR PRiMA 170952 N-linked glycosylation of proline-rich membrane anchor (PRiMA) is not required for assembly and trafficking of globular tetrameric acetylcholinesterase. 31471319 8 24 gly N-glycosylated 1099:1112 arg1 ZNT1 ZNT1 PUBTATOR ZNT1 7779 ZNT1 is N-glycosylated on Asn299 in the extracellular loop between transmembrane domains V and VI, and this appears to be involved in the regulation of ZNT1 stability, as nonglycosylated ZNT1 is more stable. 31471319 8 28 gly nonglycosylated 1262:1276 arg1 nonglycosylated ZNT1 nonglycosylated ZNT1 PUBTATOR ZNT1 7779 ZNT1 is N-glycosylated on Asn299 in the extracellular loop between transmembrane domains V and VI, and this appears to be involved in the regulation of ZNT1 stability, as nonglycosylated ZNT1 is more stable. 17286803 5 39 gly non-glycosylated 585:600 arg1 Both partially and non-glycosylated CLN3 Both partially and non-glycosylated CLN3 OGER CLN3 Q13286 Both partially and non-glycosylated CLN3 were transported correctly to lysosomes. 8617200 5 66 part_of beta1-LAP 992:1000 arg1 Cys33 LAP Cys33 OGER AminoAcid LAP P17676 Cys33 The cysteine required for the association between LTBP-1 and beta1-LAP was mapped to Cys33 of beta1-LAP. 20511397 2 13 gly glycosylated/sialylated 523:545 arg1 plasma apoE plasma apoE PUBTATOR apoE 348 Cellular apoE and plasma apoE exist as multiple glycosylated and sialylated glycoforms with plasma apoE being less glycosylated/sialylated than cell-derived apoE. 20511397 1 7 gly glycoprotein 209:220 arg1 Apolipoprotein E Apolipoprotein E PUBTATOR Apolipoprotein E 348 Apolipoprotein E (apoE) is a 34-kDa glycoprotein secreted from various cells including hepatocytes and macrophages and plays an important role in remnant lipoprotein clearance, immune responses, Alzheimer disease, and atherosclerosis. 8702538 10 29 gly glycoproteins 1714:1726 arg1 the CD22 and CD33 glycoproteins the CD22 and CD33 glycoproteins OGER CD33 glycoproteins P20138 These observations suggest that a single N-linked glycosylation site located at a similar position in the CD22 and CD33 glycoproteins is critical for regulating ligand recognition by both receptors. 17650508 1 14 gly glycoprotein 222:233 arg1 myocilin myocilin PUBTATOR myocilin 4653 MYOC, a gene involved in different types of glaucoma, encodes myocilin, a secreted glycoprotein of unknown function, consisting of an N-terminal leucine-zipper-like domain, a central linker region, and a C-terminal olfactomedin-like domain. 34234349 4 36 gly α1 682:683 arg1 IV 1 IV PUBTATOR 1 28881 Ziconotide is thoroughly coordinated by helices P1 and P2, which support the selectivity filter, and the extracellular loops (ECLs) in repeats II, III and IV of α1. 34234349 4 36 gly α1 682:683 arg1 repeats II, III and IV 1 repeats II, III and IV PUBTATOR 1 28881 Ziconotide is thoroughly coordinated by helices P1 and P2, which support the selectivity filter, and the extracellular loops (ECLs) in repeats II, III and IV of α1. 34234349 4 36 gly α1 682:683 arg1 II 1 II PUBTATOR 1 28881 Ziconotide is thoroughly coordinated by helices P1 and P2, which support the selectivity filter, and the extracellular loops (ECLs) in repeats II, III and IV of α1. 22547800 4 20 gly channel 791:797 arg1 a leucine-rich repeat (LRR)-containing membrane protein BK channel a leucine-rich repeat (LRR)-containing membrane protein OGER BK channel We recently identified a leucine-rich repeat (LRR)-containing membrane protein, LRRC26, as a BK channel auxiliary subunit, which causes an unprecedented large negative shift (∼140 mV) in voltage dependence of channel activation. 22547800 4 23 gly BK 788:789 arg1 a leucine-rich repeat (LRR)-containing membrane protein BK channel a leucine-rich repeat (LRR)-containing membrane protein OGER BK channel We recently identified a leucine-rich repeat (LRR)-containing membrane protein, LRRC26, as a BK channel auxiliary subunit, which causes an unprecedented large negative shift (∼140 mV) in voltage dependence of channel activation. 1694179 12 37 gly glycosylated 1694:1705 arg1 protein C protein C OGER protein C P02810 The percentage of protein C that is glycosylated at this site may therefore depend at least in part on the rate of disulfide bond formation which may in turn be related to the rate of protein synthesis. 22023369 2 33 gly nonfucosylated 409:422 arg1 nonfucosylated IgG1-Fc nonfucosylated IgG1-Fc OGER IgG1 P01857 Here, we present the 2.2-Å structure of the complex formed between nonfucosylated IgG1-Fc and a soluble form of FcγRIIIa (sFcγRIIIa) with two N-glycosylation sites. 28668641 1 34 gly glycoprotein 128:139 arg1 Vaspin Vaspin PUBTATOR Vaspin 145264 Vaspin is a glycoprotein with three predicted glycosylation sites at asparagine residues located in proximity to the reactive center loop and close to domains that play important roles in conformational changes underlying serpin function. 11562499 3 33 gly glycosylated 539:550 arg1 glycosylated human PEDF glycosylated human PEDF PUBTATOR PEDF 5176 To provide a structural basis for understanding its many biological roles, we have solved the crystal structure of glycosylated human PEDF to 2.85 A. 19855092 8 64 gly desialylated 1300:1311 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin 11450 In contrast, plasma clearance of desialylated adiponectin was accelerated compared with that of control adiponectin, implicating a role for this modification in determining the half-life of circulating adiponectin. 22387313 3 52 gly glycoprotein 484:495 arg1 DPP10 DPP10 PUBTATOR DPP10 57628 DPP10 is a glycoprotein containing eight predicted N-glycosylation sites in the extracellular domain. 31959827 12 44 gly O-glycoforms 1521:1532 arg1 pathogenic IgA1 HR O-glycoforms pathogenic IgA1 HR O-glycoforms PUBTATOR IgA1 P01876 The new workflow for quantitative profiling of IgA1 HR O-glycoforms with site-specific resolution will enable identification of pathogenic IgA1 HR O-glycoforms in IgAN. 31959827 12 45 gly O-glycoforms 1429:1440 arg1 IgA1 HR O-glycoforms IgA1 HR O-glycoforms PUBTATOR IgA1 P01876 The new workflow for quantitative profiling of IgA1 HR O-glycoforms with site-specific resolution will enable identification of pathogenic IgA1 HR O-glycoforms in IgAN. 17711303 1 60 gly glycosylation 144:156 arg1 FLAG-hKOR FLAG-hKOR PUBTATOR hKOR 4986 We examined glycosylation of FLAG-hKOR expressed in CHO cells and determined its functional significance. 10978165 6 14 gly glycosylated 659:670 arg1 glycosylated HCC-1 HCC-1 (1-74 PUBTATOR HCC-1 (1-74 6358 These peptides were isolated from a peptide library of human blood filtrate and represent predominantly HCC-1 (1-74) and glycosylated HCC-1 (1-74). 14699159 0 90 gly Underglycosylation 0:17 arg1 ATF6 ATF6 PUBTATOR ATF6 22926 Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response. 2243102 3 56 gly polylactosaminoglycans 306:327 arg1 lamp-2 lamp-2 polylactosaminoglycans PUBTATOR lamp-2 3920 We have localized the polylactosaminoglycans to specific sites on lamp-1 and lamp-2 purified from human chronic myelogenous leukemia cells. 2243102 3 56 gly polylactosaminoglycans 306:327 arg1 lamp-1 lamp-1 polylactosaminoglycans PUBTATOR lamp-1 3916 We have localized the polylactosaminoglycans to specific sites on lamp-1 and lamp-2 purified from human chronic myelogenous leukemia cells. 2243102 6 49 gly located 942:948 arg1 lamp-2 AND polylactosaminoglycans lamp-2 polylactosaminoglycans PUBTATOR lamp-2 3920 Amino acid analysis and sequencing demonstrated that polylactosaminoglycans were located at Asn-34, Asn-93 and/or Asn-102, and Asn-195 and/or Asn-200 in lamp-1, and at Asn-4 and/or Asn-10, and Asn-279 in lamp-2. 24927598 7 26 gly glycosylation 953:965 arg1 ZIP14 ZIP14 PUBTATOR ZIP14 23516 Asparagine-linked (N-linked) glycosylation of ZIP14, particularly the glycosylation at N102, was required for efficient membrane extraction of ZIP14 and therefore is necessary for its iron sensitivity. 19467646 9 51 gly glycosylated 1169:1180 arg1 BDNF BDNF PUBTATOR BDNF 627 Treatment with N-glycanase and plasmin reduced the size of the higher molecular weight bands, confirming the glycosylated pro-form of BDNF. 17711303 0 82 gly N-Glycosylation 0:14 arg1 the human kappa opioid receptor the human kappa opioid receptor PUBTATOR kappa opioid receptor 4986 N-Glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway. 15044018 3 11 gly glycoprotein 589:600 arg1 IGRP IGRP PUBTATOR IGRP 57818 We show that IGRP is a glycoprotein, held in the endoplasmic reticulum by nine transmembrane domains, which is degraded in cells predominantly through the proteasome pathway that generates the major histocompatibility complex class I-presented peptides. 11502179 1 68 gly containing 283:292 arg1 the human TSH receptor AND a 10-residue histidine tag the human TSH receptor The amino-terminal ectodomain a 10-residue histidine tag PUBTATOR TSH receptor 7253 ectodomain The amino-terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a glycosylphosphatidylinositol-anchored molecule containing a 10-residue histidine tag close to its C terminus. 11080501 0 106 gly N-glycosylation 48:62 arg1 the human gastrointestinal carcinoma antigen GA733-2 the human gastrointestinal carcinoma antigen GA733-2 OGER GA733-2 P16422 Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM). 24121512 6 21 part_of cdAE1 1228:1232 arg1 55-356 cdAE1 55-356 Cterm SpecificSite cdAE1 6521 residues 55-356 A 2.1-Å resolution crystal structure of cdΔ54AE1 (residues 55-356 of cdAE1) lacking the amino-terminal and carboxyl-terminal disordered regions, produced at physiological pH, revealed an extensive hydrogen-bonded network involving Arg(283) and Glu(85). 10201933 1 19 gly glycoprotein 210:221 arg1 CDw108 CDw108 PUBTATOR CDw108 8482 CDw108, also known as the John-Milton-Hagen human blood group Ag, is an 80-kDa glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that is preferentially expressed on activated lymphocytes and E. 11741940 8 32 gly moiety 1571:1576 arg1 Asn Asn Asn SpecificSite Asn(172) Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 11741940 8 32 gly moiety 1571:1576 arg1 172 172 172 SpecificSite Asn(172) Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 11741940 8 87 gly contains 1530:1537 arg1 sFRP-1 AND a relatively large carbohydrate moiety sFRP-1 a relatively large carbohydrate moiety PUBTATOR sFRP-1 6422 Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 17715132 5 1 gly glycosylated 694:705 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We show for the first time that Pannexin1 is glycosylated at Asn-254 and that this residue is important for plasma membrane targeting. 21056543 1 2 gly glycoprotein 129:140 arg1 Protein C inhibitor Protein C inhibitor OGER Protein C inhibitor P05154 Protein C inhibitor (PCI) is a 57-kDa glycoprotein that exists in many tissues and secretions in human. 16169851 6 14 gly glycoprotein 767:778 arg1 herpes virus glycoprotein herpes virus glycoprotein PUBTATOR glycoprotein D 2532 Moreover, the structure shows that BTLA recognizes the same surface on HVEM as gD (herpes virus glycoprotein D) and utilizes a similar binding motif. 2129367 5 69 part_of IX 639:640 arg1 Ser-53 factor IX Ser-53 PUBTATOR SpecificSite factor IX 2158 Ser-53 The same results were also obtained for a pentapeptide containing Ser-53 of factor IX and protein Z. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. 20427278 5 15 gly O-glycosylation 678:692 arg1 APP APP OGER APP P05067 TMEM59 transfection inhibited complex N- and O-glycosylation of APP in cultured cells. 2498325 3 57 gly apoE 269:272 arg1 The carbohydrate attachment site apoE The carbohydrate attachment site PUBTATOR apoE 348 The carbohydrate attachment site of plasma apoE was localized to a single tryptic peptide (residues 192-206). 2963625 5 40 gly H 720:720 arg1 the seventh homologous repeat unit factor H the seventh homologous repeat unit PUBTATOR factor H 3075 A tyrosine/histidine polymorphism was observed within the seventh homologous repeat unit of factor H. 15863501 9 48 gly N-glycosylation 1604:1618 arg1 sPLA(2)-III sPLA(2)-III PUBTATOR sPLA(2)-III 50487 Taken together, these results reveal unique cell type-specific processing and N-glycosylation of sPLA(2)-III and the potential role of this enzyme in cancer development by stimulating tumor cell growth and angiogenesis. 14718370 10 72 gly C-mannosylated 1663:1676 arg1 MUC5B MUC5B PUBTATOR MUC5B Q9HC84 Considered together, these studies suggest that the Cys subdomains of MUC5AC and MUC5B are C-mannosylated in their respective WXXW motifs. 14718370 10 72 gly C-mannosylated 1663:1676 arg1 MUC5AC MUC5AC PUBTATOR MUC5AC P98088 Considered together, these studies suggest that the Cys subdomains of MUC5AC and MUC5B are C-mannosylated in their respective WXXW motifs. 21908432 6 20 part_of GPIbβ 822:826 arg1 residue Tyr106 GPIbβ, residue Tyr106 PUBTATOR AminoAcid GPIbβ, 2811 residue Tyr106 Central to this interface is residue Tyr106 from GPIbβ, which inserts into a pocket generated by 2 loops (b,c) from GPIX. 19196183 8 44 gly N-glycosylated 1206:1219 arg1 bovine serum albumin bovine serum albumin OGER albumin P02768 Furthermore, unavoidable contaminants such as actin and bovine serum albumin which are not N-glycosylated could be easily depleted by using this glycoproteomic strategy. 21712440 2 49 gly glycoprotein 411:422 arg1 APP APP OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 21712440 2 6 gly carrying 424:431 arg1 APP AND O-glycans APP O-glycans OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 21712440 2 6 gly carrying 424:431 arg1 APP AND N- APP N- OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 25765764 1 42 gly LRIG1-ECD 129:137 arg1 the LRIG1-LRR (leucine-rich repeat) domain LRIG1 the LRIG1-LRR (leucine-rich repeat) domain PUBTATOR LRIG1 26018 We have expressed and purified three soluble fragments of the human LRIG1-ECD (extracellular domain): the LRIG1-LRR (leucine-rich repeat) domain, the LRIG1-3Ig (immunoglobulin-like) domain, and the LRIG1-LRR-1Ig fragment using baculovirus vectors in insect cells. 31959827 3 25 gly glycoforms 392:401 arg1 IgA1 glycoforms IgA1 glycoforms PUBTATOR IgA1 P01876 IgA1 glycoforms with some galactose-deficient (Gd) HR O-glycans play a key role in IgAN pathogenesis. 21752865 11 20 gly N-glycosylated 1756:1769 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Our results confirm the theoretical predictions that BRI2 is N-glycosylated at Asn170 and show that this post-translational modification is essential for its expression at the cell surface but not for its proteolytic processing.