doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_base_np	arg_protein	arg_domain	arg_site	arg_sugar	PSource	SiteSource	NProtein	NID	SiteName	sent_text
36482940	2	32	gly	N-glycopeptide	524:537	arg2	the site- and structure-specific intact N-glycopeptide search engine GPSeeker			the site- and structure-specific intact N-glycopeptide search engine GPSeeker						N-glycopeptide	Methods: C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets.
36482940	2	94	gly	N-glycopeptides	435:449	arg2	labeled intact N-glycopeptides			labeled intact N-glycopeptides						N-glycopeptides	Methods: C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets.
35887202	3	5	gly	O-glycan	495:502	arg1	a specific Thr			a specific Thr	a specific Thr		AminoAcid			Thr	MY.1E12 is a promising anti-MUC1 antibody with a distinct specificity toward MUC1 modified with an immature O-glycan (NeuAcα(2-3)Galβ(1-3)GalNAc) on a specific Thr.
33073321	1	12	gly	Zr-MOF	252:257	arg1	A hydrophilic carbohydrate functionalized magnetic metal organic framework	MOF@G6P			A hydrophilic carbohydrate functionalized magnetic metal organic framework	OGER		MOF@G6P	Q9H7Z6		A hydrophilic carbohydrate functionalized magnetic metal organic framework (Mag Zr-MOF@G6P) was synthesized via a facile one-step modification strategy for selective glycopeptide capture in virtue of hydrophilic interaction chromatography technique.
33073321	1	21	gly	glycopeptide	338:349	arg2	selective glycopeptide capture			selective glycopeptide capture						glycopeptide	A hydrophilic carbohydrate functionalized magnetic metal organic framework (Mag Zr-MOF@G6P) was synthesized via a facile one-step modification strategy for selective glycopeptide capture in virtue of hydrophilic interaction chromatography technique.
33073321	1	54	gly	@	258:258	arg1	A hydrophilic carbohydrate functionalized magnetic metal organic framework	MOF@G6P			A hydrophilic carbohydrate functionalized magnetic metal organic framework	OGER		MOF@G6P	Q9H7Z6		A hydrophilic carbohydrate functionalized magnetic metal organic framework (Mag Zr-MOF@G6P) was synthesized via a facile one-step modification strategy for selective glycopeptide capture in virtue of hydrophilic interaction chromatography technique.
33073321	1	71	gly	Mag	248:250	arg1	A hydrophilic carbohydrate functionalized magnetic metal organic framework	Mag			A hydrophilic carbohydrate functionalized magnetic metal organic framework	OGER		Mag	P20916		A hydrophilic carbohydrate functionalized magnetic metal organic framework (Mag Zr-MOF@G6P) was synthesized via a facile one-step modification strategy for selective glycopeptide capture in virtue of hydrophilic interaction chromatography technique.
35977913	4	19	gly	N-acetylglucosamine	537:555	arg1	proteins	proteins			N-acetylglucosamine	Fterm		proteins			Utilizing the endoglycosidase-catalyzed transglycosylation method, a single N-acetylglucosamine (N-GlcNAc, analogous to a tree stump) on proteins can be converted to various homogeneous N-glycosylated forms, thereby becoming the focus of research efforts.
37289534	4	11	gly	head	845:848	arg1	four or two saccharide head groups				four or two saccharide head groups						The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively.
35414481	12	78	gly	fucosylated	1732:1742	arg1	tri-antennary fucosylated glycans				tri-antennary fucosylated glycans						The largest change over time was observed for tri-antennary fucosylated glycans, which were able to differentiate cases from controls with a specificity of 92%, sensitivity of 49% and accuracy of 90%.
33161144	2	23	gly	glycosylation	375:387	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			It has been reported by our group and by others that different carbon sources, such as glucose, mannose and galactose, can differently impact the glycosylation profile of glycoproteins in mammalian cell culture.
33161144	2	41	gly	glycoproteins	400:412	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			It has been reported by our group and by others that different carbon sources, such as glucose, mannose and galactose, can differently impact the glycosylation profile of glycoproteins in mammalian cell culture.
36329887	4	10	gly	F1-deglycosylated	711:727	arg1	Endoglycosidase F1-deglycosylated FSH	Endoglycosidase F1-deglycosylated FSH				OGER		FSH			Endoglycosidase F1-deglycosylated FSH bound to the complete extracellular domain of the FSH receptor crystallized as a trimeric complex.
36422041	10	62	gly	wall	1803:1806	arg1	composition			composition						position,	In conclusion, S. schenckii ROT2 is required for proper N-linked glycosylation, cell wall organization and composition, and interaction with the host.
35393560	2	0	gly	glycoproteins	194:206	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			Frequently, therapeutic glycoproteins exhibit a heterogeneous array of glycans that are intended to mimic human glycopatterns.
36830744	0	34	gly	N-Glycosylation	4:18	arg1	Coronary Artery Disease	Coronary Artery Disease				Fterm		Disease			IgG N-Glycosylation Is Altered in Coronary Artery Disease.
35995381	14	78	gly	sites	2060:2064	arg1	the sites Asn110, Asn1869, and Asn2122			sites Asn110, Asn1869, and Asn2122				thyroglobulin	7038	sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn1869			sites Asn110, Asn1869, and Asn2122				thyroglobulin	7038	sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn2122			sites Asn110, Asn1869, and Asn2122				thyroglobulin	7038	sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn1869			sites Asn110, Asn1869, and Asn2122				thyroglobulin	7038	sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn2122			sites Asn110, Asn1869, and Asn2122				thyroglobulin	7038	sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn2122			sites Asn110, Asn1869, and Asn2122				thyroglobulin	7038	sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35405095	5	36	gly	carrying	920:927	arg1	adjacent residues AND truncated core 1 O-glycans			adjacent residues	truncated core 1 O-glycans					residues	Here, we focus on the mucin-selective metalloprotease, Amuc_0627 (AM0627), which is known to cleave between adjacent residues carrying truncated core 1 O-glycans.
35460266	5	19	gly	regions	793:799	arg1	neutral polysaccharides			regions	neutral polysaccharides					regions	In this study, neutral polysaccharides of PR from four different regions of China (Chun'an (Zhejiang), Xixia (Henan), Danfeng (Shanxi), and Pan'an (Zhejiang)), named CAZJ, XXHN, DFSX, and PAZJ, respectively, were isolated by anion-exchange and gel-permeation chromatography.
35737823	1	15	gly	glycoprotein	192:203	arg1	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein				Fterm		glycoprotein			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein is the prime target for vaccines, diagnostics, and therapeutic antibodies against the virus.
36233110	0	86	gly	Protein	73:79	arg1	Site-Specific Glycan Microheterogeneity Evaluation	Protein			Site-Specific Glycan Microheterogeneity Evaluation	Fterm		Protein			Site-Specific Glycan Microheterogeneity Evaluation of Aflibercept Fusion Protein by Glycopeptide-Based LC-MSMS Mapping.
32417172	7	19	gly	glycosylated	1157:1168	arg1	the glycosylated sites			the glycosylated sites						sites	Using mass spectrometry analysis and site-directed mutagenesis, we identified the glycosylated sites and studied the functions of O-GalNAc glycosylation on p53.
37294165	10	24	gly	non-glycosylated	1626:1641	arg1	non-glycosylated SPINK13	non-glycosylated SPINK13				OGER		SPINK13	Q1W4C9		Invasion assays of glycosylated SPINK13 and non-glycosylated SPINK13 with pancreatic cancer cells showed that non-glycosylated SPINK-13 was more potent than that of glycosylated SPINK13.
37294165	10	45	gly	glycosylated	1747:1758	arg1	glycosylated SPINK13	glycosylated SPINK13				OGER		SPINK13	Q1W4C9		Invasion assays of glycosylated SPINK13 and non-glycosylated SPINK13 with pancreatic cancer cells showed that non-glycosylated SPINK-13 was more potent than that of glycosylated SPINK13.
37294165	10	54	gly	non-glycosylated	1692:1707	arg1	non-glycosylated SPINK-13	non-glycosylated SPINK-13				OGER		SPINK-13	Q1W4C9		Invasion assays of glycosylated SPINK13 and non-glycosylated SPINK13 with pancreatic cancer cells showed that non-glycosylated SPINK-13 was more potent than that of glycosylated SPINK13.
37294165	10	69	gly	glycosylated	1601:1612	arg1	glycosylated SPINK13	glycosylated SPINK13				OGER		SPINK13	Q1W4C9		Invasion assays of glycosylated SPINK13 and non-glycosylated SPINK13 with pancreatic cancer cells showed that non-glycosylated SPINK-13 was more potent than that of glycosylated SPINK13.
36812846	1	12	gly	glycoproteins	134:146	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Oligomannose-type glycans on glycoproteins are important signaling molecules in the glycoprotein quality control system in the endoplasmic reticulum.
36812846	1	55	gly	glycoprotein	189:200	arg1	the glycoprotein quality control system	the glycoprotein quality control system				Fterm		glycoprotein			Oligomannose-type glycans on glycoproteins are important signaling molecules in the glycoprotein quality control system in the endoplasmic reticulum.
36812846	1	63	gly	glycans	123:129	arg1	glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			Oligomannose-type glycans on glycoproteins are important signaling molecules in the glycoprotein quality control system in the endoplasmic reticulum.
33493676	10	5	gly	branching	1896:1904	arg1	AGP's second glycosylation site			AGP's second glycosylation site	AGP's second glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	5	gly	branching	1896:1904	arg1	AGP's third and AGP1's fourth glycosylation site			AGP's third and AGP1's fourth glycosylation site	AGP's third and AGP1's fourth glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	10	gly	glycosylation	1922:1934	arg2	AGP's second glycosylation site			AGP's second glycosylation site						site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	86	gly	glycosylation	2009:2021	arg2	AGP's third and AGP1's fourth glycosylation site			AGP's third and AGP1's fourth glycosylation site						site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's second glycosylation site			site	AGP's second glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site	AGP's second glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's second glycosylation site			site	AGP's third and AGP1's fourth glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site	AGP's third and AGP1's fourth glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's second glycosylation site			site	N-glycans					site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site	N-glycans					site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site						site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
34017182	5	65	gly	glycosylated	1162:1173	arg1	post-translational modified proteins	post-translational modified proteins				Fterm		proteins			Moreover, post-translational modified proteins (phosphorylated and N-linked glycosylated) were studied.
36409896	2	33	gly	glycoforms	219:228	arg1	complex protein glycoforms				complex protein glycoforms						However, interrogating complex protein glycoforms is challenging, as current lectin tools are limited by cross-reactivity while mass spectrometry typically requires biochemical purification and isolation of the target protein.
36849074	2	7	gly	used	405:408	arg2	Composites			Composites						Composites	Composites within 0.08-0.25 wt% medium-viscosity alginate were used, at fixed PLA, 6.6 wt%, compared with a study using 0.17-0.48 wt% low-viscosity alginate (same PLA), starting from water-in-oil emulsions, before FS.
32531122	8	21	gly	IgG	1015:1017	arg1	N-linked glycosyl residues	IgG			N-linked glycosyl residues	Cterm		IgG			Furthermore, we show that removal of N-linked glycosyl residues from these IgG did not interfere with its entry into the podocytes but eliminated its ability to upregulate CAMK4 and cause podocyte injury.
35945033	1	7	gly	glycoproteins	224:236	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The analysis of glycoproteins and the comparison of protein N-glycosylation from different eukaryotic origins require unbiased and robust analytical workflows.
31945375	7	55	gly	d-mannopyranose	1282:1296	arg1	the -1 subsite			the -1 subsite	the -1 subsite		Site			subsite	In addition, using two differential derivatization methods, we have shown that there is an absolute requirement for undecorated d-mannopyranose in the -1 subsite.
34957216	10	23	gly	glycoprotein	1552:1563	arg1	the S glycoprotein	the S glycoprotein				PUBTATOR		S glycoprotein	43740568		We further predicted that many mutations on N-linked glycosylation sites would increase the stability of the S glycoprotein.
34957216	10	54	gly	glycosylation	1494:1506	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	We further predicted that many mutations on N-linked glycosylation sites would increase the stability of the S glycoprotein.
34102146	5	28	gly	glycopeptides	1015:1027	arg2	S- glycopeptides	proteins		glycopeptides		Fterm		proteins		glycopeptides	In total, our spatially resolved glycoproteomics technique identified over 400 N-, O-, and S- glycopeptides from over 30 proteins, demonstrating the diverse array of glycosylation present on the tissue slices and the sensitivity of our technique.
34778211	2	83	gly	presence	293:300	arg2	antibody Fc region AND core fucose			antibody Fc region	core fucose					region	The presence of core fucose on antibody Fc region can inhibit antibody-dependent cellular cytotoxicity (ADCC) and reduce antibody therapeutic efficiency in vivo.
33592173	5	16	gly	glycosylates	766:777	arg1	TOM70	TOM70				OGER		TOM70	O94826		Phosphorylated OGT glycosylates TOM70 on Ser94, enhancing MIC19 protein import into mitochondria and promoting cristae formation and respiration.
36377874	0	13	gly	Deletions	69:77	arg1	the Envelope Protein	Envelope Protein			Deletions	PUBTATOR		Envelope Protein	64006		Pathogenicity and Structural Basis of Zika Variants with Glycan Loop Deletions in the Envelope Protein.
36824920	1	36	gly	glycoproteins	119:131	arg1	Coronavirus spike glycoproteins	Coronavirus spike glycoproteins				Fterm		glycoproteins			Coronavirus spike glycoproteins presented on the virion surface mediate receptor binding, and membrane fusion during virus entry and constitute the primary target for vaccine and drug development.
35309324	7	30	gly	glycosylation	1286:1298	arg1	PD-1	PD-1				PUBTATOR		PD-1	Q15116		These results indicate that both the binding and blocking efficacy of cemiplimab require the N58 glycosylation of PD-1.
34399822	10	92	gly	enzymes	1419:1425	arg1	O-GlcNAcylation	enzymes			O-GlcNAcylation	Fterm		enzymes			Finally, the key enzymes in O-GlcNAcylation: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were examined and molecular docking analysis was used to determine effective natural compounds in the regulation on OGT and OGA activities.
35309180	14	47	gly	glycoproteins	1459:1471	arg1	The N-linked glycans	glycoproteins			The N-linked glycans	Fterm		glycoproteins			The N-linked glycans of glycoproteins were assessed to reveal chemotaxonomic patterns.
34012659	15	96	gly	N-glycosylation	2040:2054	arg1	BST-2	BST-2				PUBTATOR		BST-2	684		CONCLUSIONS Our findings illuminate the novel function of BST-2 as an oncogene of HBV-associated HCC, and highlight the novel relationship of N-glycosylation of BST-2 in regulating HCC tumorigenesis in vitro.
36409896	0	4	gly	glycoforms	52:61	arg1	IgG Fc glycoforms	IgG Fc glycoforms				Cterm		IgG			Synthetic nanobodies as tools to distinguish IgG Fc glycoforms.
35662639	3	66	gly	glycoproteins	569:581	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We have shown that Bothrops venoms are markedly defined by their content of glycoproteins, and that most N-glycan structures of eight Bothrops venoms contain sialic acid, while bisected N-acetylglucosamine was identified in Bothrops cotiara venom.
34878920	7	23	gly	glycosylated	1126:1137	arg1	the heavily glycosylated envelope protein	the heavily glycosylated envelope protein				PUBTATOR		envelope protein	64006		Among all the viral proteins, the heavily glycosylated envelope protein is especially crucial.
36478308	10	14	gly	sites	1431:1435	arg1	N234			sites N61, N234				SARS	P49591	sites N61, N234	The majority of the glycans present were of the truncated class, although at sites N61, N234, and N717/714 high mannose structures were dominant and at N1173/1170 aglycosylation was dominant for both variant proteins.
36478308	10	14	gly	sites	1431:1435	arg1	N61			sites N61, N234				SARS	P49591	sites N61, N234	The majority of the glycans present were of the truncated class, although at sites N61, N234, and N717/714 high mannose structures were dominant and at N1173/1170 aglycosylation was dominant for both variant proteins.
36478308	10	14	gly	sites	1431:1435	arg1	N61			sites N61, N234				SARS	P49591	sites N61, N234	The majority of the glycans present were of the truncated class, although at sites N61, N234, and N717/714 high mannose structures were dominant and at N1173/1170 aglycosylation was dominant for both variant proteins.
33085102	2	21	gly	glycoprotein	342:353	arg1	different glycoprotein profiles	different glycoprotein profiles				Fterm		glycoprotein			This study reveals different glycoprotein profiles obtained from human, bovine, and caprine milk and their potential roles in supporting infant growth.
36095241	3	6	gly	glycopeptides	564:576	arg2	homogeneous O-linked glycopeptides			homogeneous O-linked glycopeptides						glycopeptides	Herein, an intrinsic O-glycosylated peptide P320-334 derived from RBD was screened and homogeneous O-linked glycopeptides containing Tn (GalNAcα1-O-Ser/Thr), T (Galβ1-3GalNAcα1-O-Ser/Thr), sialyl-Tn (sTn, Siaα2-6GalNAcα1-O-Ser/Thr), and sialyl-T (sT, Siaα2-3Galβ1-3GalNAcα1-O-Ser/Thr) structures were first synthesized via chemoenzymatic strategies.
36095241	3	39	gly	O-glycosylated	477:490	arg1	an intrinsic O-glycosylated peptide P320-334			an intrinsic O-glycosylated peptide P320-334						peptide	Herein, an intrinsic O-glycosylated peptide P320-334 derived from RBD was screened and homogeneous O-linked glycopeptides containing Tn (GalNAcα1-O-Ser/Thr), T (Galβ1-3GalNAcα1-O-Ser/Thr), sialyl-Tn (sTn, Siaα2-6GalNAcα1-O-Ser/Thr), and sialyl-T (sT, Siaα2-3Galβ1-3GalNAcα1-O-Ser/Thr) structures were first synthesized via chemoenzymatic strategies.
32409323	5	73	gly	FBXO6	701:705	arg1	transgenic Col2a1-CreER mice	FBXO6			transgenic Col2a1-CreER mice	PUBTATOR		FBXO6	50762		The role of FBXO6 in cartilage degeneration was analysed with global FBXO6 mice, transgenic Col2a1-CreER mice.
32592613	7	50	gly	glycosylation	1160:1172	arg1	IGFBP-3	IGFBP-3				OGER		IGFBP-3	P17936		Binding of IGFBP-3 to either HA or HN was unaffected by glycosylation or reduction of IGFBP-3, suggesting that the basic 18-amino acid residue sequence of IGFBP-3 remains accessible for interaction with either HN or HA upon glycosylation or reduction of the full-length protein.
35481895	1	43	gly	glycosylation	136:148	arg1	structural proteins	structural proteins				Fterm		proteins			The glycosylation of structural proteins is a widespread posttranslational modification in Archaea.
32273875	5	88	gly	glycosylation	1014:1026	arg2	the N-linked glycosylation site			the N-linked glycosylation site						site	Production of the corresponding recombinant FVIII mutants or light chains indicated that removal of the N-linked glycosylation site at N2118 is sufficient to abrogate in vitro the activation of FVIII-specific CD4+ T cells by human monocyte-derived dendritic cells.
35713525	6	24	gly	N-glycan	731:738	arg1	Asn-224	Sur1		Asn-224	Asn-224		SpecificSite	Sur1	856050	Asn-224	Sur1 carries an N-glycan on Asn-224, whereas Csh1 has N-glycans on Asn-51 and Asn-247.
35713525	6	8	gly	carries	720:726	arg1	Sur1 AND an N-glycan	Sur1			an N-glycan	PUBTATOR		Sur1	856050		Sur1 carries an N-glycan on Asn-224, whereas Csh1 has N-glycans on Asn-51 and Asn-247.
35713525	6	46	gly	has	765:767	arg1	Csh1 AND N-glycans	Csh1			N-glycans	PUBTATOR		Csh1	852458		Sur1 carries an N-glycan on Asn-224, whereas Csh1 has N-glycans on Asn-51 and Asn-247.
36824920	6	52	gly	glycosylation	1174:1186	arg2	N1242			N1242						N1242	We show that glycosylation at N1242 at the upper portion of the stalk is responsible for the extensive orientational freedom of the spike crown.
35364207	0	61	gly	glycosylation	35:47	arg1	lactoferrin	lactoferrin				OGER		lactoferrin	P02788		Effect of chitosan oligosaccharide glycosylation on the emulsifying property of lactoferrin.
32417172	4	31	gly	glycosylated	573:584	arg1	the tumor suppressor p53	the tumor suppressor p53				OGER		tumor suppressor p53	P04637		Previously, we reported the tumor suppressor p53 could be O-GalNAc glycosylated in vitro.
36385894	6	57	gly	sialylated	1097:1106	arg1	The N-glycans				The N-glycans						The N-glycans were mostly sialylated and sialofucosylated branched structures.
36329887	7	100	gly	derived	1365:1371	arg1	each FSH subunit AND Highly purified hFSH oligosaccharides	each FSH subunit			Highly purified hFSH oligosaccharides	Fterm		subunit			Highly purified hFSH oligosaccharides derived from each FSH subunit, were characterized by electrospray ionization-ion mobility-collision-induced dissociation (ESI-IM-CID) mass spectrometry.
34452239	3	31	gly	glycopeptide	715:726	arg2	The related O-linked glycopeptide			The related O-linked glycopeptide						glycopeptide	The related O-linked glycopeptide, Asp-Arg-Val-Tyr-Ile-His-Ser-(O-β-D-Glc)-amide (PNA5), is a biousian revision of the native peptide hormone Ang (1-7) and shows enhanced stability in vivo and greater levels of brain penetration.
34274643	7	60	gly	fucosylation	1180:1191	arg1	the N-linked glycans				the N-linked glycans						Results from these separation methods indicated that fucosylation of the N-linked glycans was more abundant when a high degree of branching was present in AGP.
33583770	7	31	gly	glycopeptides	1514:1526	arg2	enriched glycopeptides			enriched glycopeptides						glycopeptides	Furthermore, dynamic quantitative glycoproteomic analysis with multiplexed stable isotope labeling and analysis of enriched glycopeptides with multiple fragmentation approaches identified glycoproteins modified by these regulated glycans including several integrins and growth factor receptors.
33583770	7	83	gly	glycoproteins	1578:1590	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins	16852		Furthermore, dynamic quantitative glycoproteomic analysis with multiplexed stable isotope labeling and analysis of enriched glycopeptides with multiple fragmentation approaches identified glycoproteins modified by these regulated glycans including several integrins and growth factor receptors.
36766693	6	37	gly	fibrinogen	1460:1469	arg1	increasingly exposed mannose residues	fibrinogen			increasingly exposed mannose residues	PUBTATOR		fibrinogen	2244		Since patients with ESRD are prone to cardiovascular complications and the formation of atherosclerotic plaques, one can hypothesize that fibrinogen with increasingly exposed mannose residues may contribute to the unwanted events.
36781790	4	46	gly	N-glycopeptides	986:1000	arg2	intact N-glycopeptides			intact N-glycopeptides						N-glycopeptides	The N-glycome information is then used to augment and guide the challenging reversed-phase LC-MS/MS-based profiling of intact N-glycopeptides from the same protein samples.
34735575	0	96	gly	glycosylation	33:45	arg1	SARS-CoV-2	SARS-CoV-2				OGER		SARS	P49591		Distinct shifts in site-specific glycosylation pattern of SARS-CoV-2 spike proteins associated with arising mutations in the D614G and Alpha variants.
31644407	1	8	gly	glycosylation	203:215	arg1	plasma proteins	plasma proteins				Fterm		proteins			BACKGROUND A number of human inflammatory diseases and tumors have been shown to cause alterations in the glycosylation pattern of plasma proteins in a specific manner.
36136114	9	20	gly	glycopeptides	1463:1475	arg2	ulinastatin glycopeptides			ulinastatin glycopeptides						glycopeptides	By this methodology, we identified and characterized ulinastatin glycopeptides at the Fc domain and linker peptide.
32545589	3	46	gly	glycosylation	629:641	arg2	putative N-linked glycosylation motifs			putative N-linked glycosylation motifs						motifs	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
32545589	3	102	gly	serine	680:685	arg1	residues			residues						serine and threonine residues	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
35193013	9	15	gly	glycoproteins	1543:1555	arg1	BALB/c mouse intestinal lavages glycoproteins	BALB/c mouse intestinal lavages glycoproteins				Fterm		glycoproteins			BALB/c mouse intestinal lavages glycoproteins contained asialo N-glycans.
35193013	9	25	gly	contained	1557:1565	arg1	BALB/c mouse intestinal lavages glycoproteins AND asialo N-glycans	BALB/c mouse intestinal lavages glycoproteins			asialo N-glycans	Fterm		glycoproteins			BALB/c mouse intestinal lavages glycoproteins contained asialo N-glycans.
36182101	6	6	gly	carries	942:948	arg1	H. pylori strain J99 AND the sialic acid-binding adhesin	H. pylori strain J99			the sialic acid-binding adhesin	Fterm		strain			H. pylori strain J99, which carries the blood group antigen-binding adhesin (BabA), the sialic acid-binding adhesin (SabA), and the LacdiNAc-binding adhesin, bound both to Lewis b (Leb)-positive and Leb-negative mucins.
36182101	6	6	gly	carries	942:948	arg1	H. pylori strain J99 AND the LacdiNAc-binding adhesin	H. pylori strain J99			the LacdiNAc-binding adhesin	Fterm		strain			H. pylori strain J99, which carries the blood group antigen-binding adhesin (BabA), the sialic acid-binding adhesin (SabA), and the LacdiNAc-binding adhesin, bound both to Lewis b (Leb)-positive and Leb-negative mucins.
35848837	4	13	gly	glycoproteins	653:665	arg1	bacterial glycoproteins	bacterial glycoproteins				Fterm		glycoproteins			However, the glycan-protein linkages in bacteria are different and there is no enzyme available to release glycans from bacterial glycoproteins.
32760395	8	87	gly	glycosylation	1789:1801	arg1	the implantation site			the implantation site						site	In both groups, this spatiotemporal variation in the glycosylation pattern of the implantation site was accompanied by corresponding changes in galectin-1 expression.
36542493	4	50	gly	glycopeptides	495:507	arg2	glycopeptides			glycopeptides						glycopeptides	Herein, we chemically synthesized glycopeptides containing this atypical glycan structure and an absolute C6 configuration through the assembly of Ser O-Hep building blocks.
36542493	4	38	gly	containing	509:518	arg1	glycopeptides AND this atypical glycan structure			glycopeptides	this atypical glycan structure					glycopeptides	Herein, we chemically synthesized glycopeptides containing this atypical glycan structure and an absolute C6 configuration through the assembly of Ser O-Hep building blocks.
33801653	5	58	gly	OGT	937:939	arg1	the O-GlcNAc-feedback regulation	OGT			the O-GlcNAc-feedback regulation	PUBTATOR		OGT	8473		In this study, we investigated the O-GlcNAc-feedback regulation of OGT and OGA expression in lung cancer cells.
36935145	1	56	gly	features	487:494	arg1	sequence			sequence						sequence	N-linked glycosylation (N-glycosylation) is a common protein post-translational modification, occurring on more than half of mammalian proteins; in striking contract with small molecule modifications (such as methylation, phosphorylation) with only single structures, N-glycosylation has multiple dimensional structural features (monosaccharide composition, sequence, linkage, anomer), which generates enormous N-glycan structures; and these structures widely regulate protein structure and functions.
35304921	4	16	gly	N-glycosylation	596:610	arg2	most potential N-glycosylation sites			most potential N-glycosylation sites						sites	Complex bi, tri, or tetraantennary N-glycans were predominant at various proportions at most potential N-glycosylation sites.
35304921	4	86	gly	predominant	543:553	arg2	most potential N-glycosylation sites AND Complex bi, tri, or tetraantennary N-glycans			most potential N-glycosylation sites	Complex bi, tri, or tetraantennary N-glycans					sites	Complex bi, tri, or tetraantennary N-glycans were predominant at various proportions at most potential N-glycosylation sites.
36852982	1	114	gly	glycosylated	278:289	arg1	other extracytoplasmic proteins	other extracytoplasmic proteins				Fterm		proteins			Neisseria meningitidis exhibits a general O-linked protein glycosylation system in which pili and other extracytoplasmic proteins are glycosylated.
34650217	5	28	gly	modified	831:838	arg3	KAT5 AND O-GlcNAcylation	KAT5			O-GlcNAcylation	OGER		KAT5	Q8CHK4		Specifically, lysine acetyltransferase 5 (KAT5), belonging to the MYST family of histone acetyltransferases (HAT), is highly modified by O-GlcNAcylation in PCK1 knockout hepatoma cells.
34972858	8	99	gly	glycoproteins	1467:1479	arg1	complex glycan profiles	glycoproteins			complex glycan profiles	Fterm		glycoproteins			Following preliminary hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection separation and analysis, glycoproteins with complex glycan profiles were subjected to further fractionation by weak anion exchange HILIC and exoglycosidase sequential digestion for cross-validation of the glycan assignment.
36482940	3	9	gly	3,733 N-glycosites	876:893	arg2	3,733 N-glycosites			3,733 N-glycosites						3,733 N-glycosites	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	11	gly	glycopeptide	729:740	arg2	13,822 glycopeptide spectral matches			13,822 glycopeptide spectral matches						glycopeptide	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	21	gly	N-glycosite	812:822	arg2	comprehensive N-glycosite			comprehensive N-glycosite						N-glycosite	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	64	gly	sequence	914:921	arg1	3,754 N-glycan sequence structures				3,754 N-glycan sequence structures						Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	86	gly	2,918 N-glycoproteins	771:791	arg1	N-glycan structure information	2,918 N-glycoproteins			N-glycan structure information	Fterm		2,918 N-glycoproteins			Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
37266972	11	38	gly	glycovariants	1357:1369	arg1	N-glycan composition	glycovariants			N-glycan composition	Fterm		glycovariants			N-glycan composition of gB glycovariants was assessed by in vitro enzymatic mobility shift assay and proven to be consistent with the expected glycoforms.
36598201	11	39	gly	N-glycosylation	1735:1749	arg1	NSP4	NSP4				OGER		NSP4	Q14B24		Taken together, the data suggest that N-glycosylation of NSP4 plays a vital role in viral replication and pathogenicity.
36709835	5	16	gly	afucosylated	872:883	arg1	afucosylated glycans				afucosylated glycans						From these studies, we observed that Remicade® had the highest percent of afucosylated glycans (15.5 ± 1.3%) and the largest number of unique glycans, 28.
36399934	9	68	gly	site	1518:1521	arg1	Arg451			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Gln360			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ser370			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Gln360			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ser370			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ser370			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
37040463	6	42	gly	glycoprotein	743:754	arg1	a cell-free glycoprotein synthesis platform	a cell-free glycoprotein synthesis platform				Fterm		glycoprotein			To address this limitation, we develop a cell-free glycoprotein synthesis platform for building noncanonical glycans and, specifically, clickable azido-sialoglycoproteins (called GlycoCAP).
37040463	6	57	gly	azido-sialoglycoproteins	838:861	arg1	clickable azido-sialoglycoproteins	clickable azido-sialoglycoproteins				Fterm		azido-sialoglycoproteins			To address this limitation, we develop a cell-free glycoprotein synthesis platform for building noncanonical glycans and, specifically, clickable azido-sialoglycoproteins (called GlycoCAP).
36565355	8	44	gly	glycosylation	875:887	arg1	the NOTCH1 signaling pathway	the NOTCH1 signaling pathway				PUBTATOR		NOTCH1	18128		To investigate the role of glycosylation in the NOTCH1 signaling pathway, nuclear magnetic resonance spectroscopy has been employed to study the structures of EGF27 and its glycoforms.
37240090	0	51	gly	Glycoproteins	47:59	arg1	the Glycan Composition	Glycoproteins			the Glycan Composition	Fterm		Glycoproteins			Alterations in the Glycan Composition of Serum Glycoproteins in Attention-Deficit Hyperactivity Disorder.
36370046	3	49	gly	protein	537:543	arg1	the glycan heterogeneity	spike protein			the glycan heterogeneity	PUBTATOR		spike protein	43740568		Analytical reports have described the glycan heterogeneity of the spike protein.
36370046	3	67	gly	heterogeneity	510:522	arg1	the spike protein	the spike protein				PUBTATOR		spike protein	43740568		Analytical reports have described the glycan heterogeneity of the spike protein.
35470665	1	0	gly	glycosylation	93:105	arg1	HIV-1	HIV-1				PUBTATOR		HIV-1) envelope protein	64006		Dense glycosylation and the trimeric conformation of the human immunodeficiency virus-1 (HIV-1) envelope protein limit the accessibility of some cellular glycan processing enzymes and end up with high-mannose-type N-linked glycans on the envelope spike, among which the Man5GlcNAc2 structure occupies a certain proportion.
37289618	2	1	gly	sialylated	398:407	arg1	intact sialylated N-linked glycans				intact sialylated N-linked glycans						Previous work has shown that infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is able to detect intact sialylated N-linked glycans without the use of chemical derivatization.
33609912	2	83	gly	glycosylation	424:436	arg1	both neutralizing and non-neutralizing IgG1	both neutralizing and non-neutralizing IgG1				OGER		IgG1	P01857		As changes in the antibody's carbohydrate chain can interfere with its effector functions, we compared the glycosylation patterns of both neutralizing and non-neutralizing IgG1 induced by pre-exposure prophylaxis to human rabies and analyzed their influence on in vitro antibody neutralizing activities.
36493594	6	38	gly	N-glycans	1109:1117	arg1	fetuin	fetuin			N-glycans	Fterm		fetuin			The N-glycans in fetuin (8-13 N-glycans were previously reported) and in IgG (19 N-glycans were previously reported), which could not be identified by using the widely used PF-AB, were all identified by using PF-ProA or PA-ProA.
36493594	6	38	gly	N-glycans	1109:1117	arg1	IgG	IgG			N-glycans	Cterm		IgG			The N-glycans in fetuin (8-13 N-glycans were previously reported) and in IgG (19 N-glycans were previously reported), which could not be identified by using the widely used PF-AB, were all identified by using PF-ProA or PA-ProA.
32791164	11	23	gly	sialylated	1865:1874	arg1	O-linked sialylated keratan sulfate chains				O-linked sialylated keratan sulfate chains						Immunoblotting, immunohistochemistry, and enzyme treatments confirmed that Siglec-8 ligand on the human airway mucus layer is an isoform of DMBT1 carrying O-linked sialylated keratan sulfate chains (DMBT1S8).
32791164	11	51	gly	carrying	1847:1854	arg1	DMBT1 AND O-linked sialylated keratan sulfate chains	DMBT1			O-linked sialylated keratan sulfate chains	OGER		isoform of DMBT1	Q9UGM3		Immunoblotting, immunohistochemistry, and enzyme treatments confirmed that Siglec-8 ligand on the human airway mucus layer is an isoform of DMBT1 carrying O-linked sialylated keratan sulfate chains (DMBT1S8).
36585837	8	65	gly	glycoproteins	1096:1108	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			ANOVA and Welch's t-test analysis identified glycoproteins differentially abundant between colostrum, transitional and mature milk (FDR <0.05).
36181511	3	14	gly	glycopeptide	648:659	arg2	overall glycopeptide enrichment			overall glycopeptide enrichment						glycopeptide	The comparison of cotton HILIC with Venusil HILIC and mixed anion-exchange (MAX) approaches indicated that cotton HILIC was superior in overall glycopeptide enrichment, whereas Venusil HILIC preferred in complex glycan structures and MAX performed better with high mannose glycans.
37186866	2	67	gly	glycosylation	708:720	arg1	these IPT domains			these IPT domains						domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
35421698	4	6	gly	3,140 N-glycoproteins	731:751	arg1	3,140 N-glycoproteins	3,140 N-glycoproteins				Fterm		3,140 N-glycoproteins			With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
35421698	4	20	gly	5,687 N-glycopeptides	682:702	arg1	3,713 N-glycosites	3,140 N-glycoproteins		3,713 N-glycosites		Fterm		3,140 N-glycoproteins		3,713 N-glycosites	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
35421698	4	20	gly	5,687 N-glycopeptides	682:702	arg2	5,687 N-glycopeptides	3,140 N-glycoproteins		5,687 N-glycopeptides		Fterm		3,140 N-glycoproteins		5,687 N-glycopeptides	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
35421698	4	28	gly	3,713 N-glycosites	709:726	arg2	3,713 N-glycosites	3,140 N-glycoproteins		3,713 N-glycosites		Fterm		3,140 N-glycoproteins		3,713 N-glycosites	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
31996426	14	105	gly	glycoprotein	2247:2258	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein M			In contrast to several respiratory viruses and bacteria, EHV1 resisted potent antimicrobial equine β-defensins (eBDs) eBD2 and eBD3 by the action of glycoprotein M. Instead, eBD2 and -3 facilitated EHV1 particle aggregation and infection of rabbit kidney (RK13) cells.
36373229	5	13	gly	O-glycopeptides	1096:1110	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	Here, we demonstrate how O-Pair Search, a recently developed O-glycopeptide-centric identification platform that enables rapid searches and confident O-glycosite localization, can be used to determine substrate specificities of various O-glycoproteases de novo from LC-MS/MS data of O-glycopeptides.
36373229	5	53	gly	O-glycosite	963:973	arg2	O-glycosite			O-glycosite						O-glycosite	Here, we demonstrate how O-Pair Search, a recently developed O-glycopeptide-centric identification platform that enables rapid searches and confident O-glycosite localization, can be used to determine substrate specificities of various O-glycoproteases de novo from LC-MS/MS data of O-glycopeptides.
36385894	8	77	gly	glycopeptides	1632:1644	arg2	glycopeptides			glycopeptides						glycopeptides	Biomarker models were constructed using serum N-glycans [area under the curve (AUC) = 0.775; 95% CI: 0.617-0.931] and glycopeptides (AUC = 0.959; 95% CI: 0.85-1.0), with glycopeptides having higher accuracies than N-glycans.
36385894	8	98	gly	glycopeptides	1684:1696	arg2	glycopeptides			glycopeptides						glycopeptides	Biomarker models were constructed using serum N-glycans [area under the curve (AUC) = 0.775; 95% CI: 0.617-0.931] and glycopeptides (AUC = 0.959; 95% CI: 0.85-1.0), with glycopeptides having higher accuracies than N-glycans.
35091091	1	56	gly	glycopeptide	178:189	arg2	complex glycopeptide samples			complex glycopeptide samples						glycopeptide	Rapidly improving methods for glycoproteomics have enabled increasingly large-scale analyses of complex glycopeptide samples, but annotating the resulting mass spectrometry data with high confidence remains a major bottleneck.
36493594	2	2	gly	glycoproteins	584:596	arg1	mammalian glycoproteins	mammalian glycoproteins				Fterm		glycoproteins			OBJECTIVE To obtain consistent results for qualitative and quantitative analyses of N-glycans, N-glycans obtained by different preparation methods were compared for two types of mammalian glycoproteins.
36253324	3	0	gly	α1,3-fucosylation	451:467	arg1	complex glycans				complex glycans						Here, we report a facile and general redox-controlled substrate engineering strategy for the site-specific α1,3-fucosylation of complex glycans containing multiple LacNAc units.
36631484	4	71	gly	glycoprotein	646:657	arg1	glycoprotein production	glycoprotein production				Fterm		glycoprotein			Notably, many factors can affect the composition of the glycans during glycoprotein production, and variations in glycosylation are among the leading causes of pharmaceutical batch rejection.
31657490	9	28	gly	glycopeptides	1527:1539	arg2	glycopeptides			glycopeptides						glycopeptides	It has great potential in the enrichment of glycopeptides and provides new ideas for synthetic materials with better enrichment properties in the future.
35279850	7	94	gly	found	1277:1281	arg2	DPM3- AND group-specific high-mannose N-glycan signatures	DPM3-			group-specific high-mannose N-glycan signatures	OGER		DPM3	Q9P2X0		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg2	ALG11- AND group-specific high-mannose N-glycan signatures	ALG11-			group-specific high-mannose N-glycan signatures	OGER		ALG11	Q2TAA5		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg2	MPDU1- AND group-specific high-mannose N-glycan signatures	MPDU1-			group-specific high-mannose N-glycan signatures	OGER		MPDU1	O75352		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg2	ALG12- AND group-specific high-mannose N-glycan signatures	ALG12-			group-specific high-mannose N-glycan signatures	OGER		ALG12	Q9BV10		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg2	DOLK- AND group-specific high-mannose N-glycan signatures	DOLK-			group-specific high-mannose N-glycan signatures	OGER		DOLK	Q9UPQ8		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg1	ALG3- AND group-specific high-mannose N-glycan signatures	ALG3-			group-specific high-mannose N-glycan signatures	OGER		ALG3	Q92685		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg1	DPM1- AND group-specific high-mannose N-glycan signatures	DPM1-			group-specific high-mannose N-glycan signatures	OGER		DPM1	O60762		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg1	ALG13-CDG AND group-specific high-mannose N-glycan signatures	ALG13-CDG			group-specific high-mannose N-glycan signatures	OGER		ALG13	Q9NP73		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg1	ALG9- AND group-specific high-mannose N-glycan signatures	ALG9-			group-specific high-mannose N-glycan signatures	OGER		ALG9	Q9H6U8		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg1	RFT1- AND group-specific high-mannose N-glycan signatures	RFT1-			group-specific high-mannose N-glycan signatures	OGER		RFT1	Q9NWF4		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
35279850	7	94	gly	found	1277:1281	arg1	SRD5A3- AND group-specific high-mannose N-glycan signatures	SRD5A3-			group-specific high-mannose N-glycan signatures	OGER		SRD5A3	Q9H8P0		Moreover, group-specific high-mannose N-glycan signatures were found in ALG3-, ALG9-, ALG11-, ALG12-, RFT1-, SRD5A3-, DOLK-, DPM1-, DPM3-, MPDU1-, ALG13-CDG, and hereditary fructose intolerance.
36159782	8	62	gly	containing	1376:1385	arg1	the IgG AND two Man5	the IgG			two Man5	Cterm		IgG			Man5 glycans resulted in decreased binding compared to complex-type glycans, with the lowest binding for the IgG containing two Man5.
36870654	1	18	gly	glycosylation	97:109	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The role of glycosylation in the binding of glycoproteins to carbohydrate substrates has not been well understood.
36870654	1	60	gly	glycoproteins	129:141	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The role of glycosylation in the binding of glycoproteins to carbohydrate substrates has not been well understood.
35943155	10	101	gly	glycoprotein	1735:1746	arg1	the glycoprotein mucin	the glycoprotein mucin				Fterm		glycoprotein			This latest enzyme also displayed activity in glycoconjugates carrying lacto-N-fucopentaose II (Lea) and lacto-N-fucopentaose III (Lex) and in the glycoprotein mucin.
32494619	4	8	gly	IRF5	648:651	arg1	O-GlcNAcylation	IRF5			O-GlcNAcylation	OGER		IRF5	Q13568		O-GlcNAcylation of IRF5 is required for K63-linked ubiquitination of IRF5 and subsequent cytokine production.
35320529	8	52	gly	glycosylation	1404:1416	arg1	endogenous membrane receptor	receptor		sites		Fterm		receptor		sites	Thus, the protocol described in this section can be utilized to identify galectin ligands at specific glycosylation sites of endogenous membrane receptor from circulating primary human lymphocytes.
35104505	0	22	gly	glycoprotein	106:117	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			N-acetylglucosaminyltransferase-V requires a specific noncatalytic luminal domain for its activity toward glycoprotein substrates.
37202422	4	33	gly	glycoforms	591:600	arg1	IgG glycoforms	IgG glycoforms				Cterm		IgG			We previously reported synthetic nanobodies that distinguish IgG glycoforms.
36478308	2	28	gly	glycosylation	407:419	arg2	single, isolated glycosylation sites			single, isolated glycosylation sites						sites	The method has been optimised to produce peptides with single, isolated glycosylation sites using multiple protease digests.
33609912	11	86	gly	deglycosylated	1767:1780	arg1	the deglycosylated IgG1	the deglycosylated IgG1				OGER		IgG1	P01857		Different glycosylation profiles were also observed in Fab and Fc fragments from neutralizing and non-neutralizing IgG1, although the deglycosylated IgG1 lost its neutralizing activity.
36056620	8	100	gly	glycosylation	1365:1377	arg2	N-linked glycosylation motifs			N-linked glycosylation motifs						motifs	CONCLUSIONS We show how N-linked glycosylation motifs can trigger dramatic changes in HCV sensitivity to nAb, independent of glycan occupancy.
33085102	7	24	gly	glycoproteins	1010:1022	arg1	17 glycoproteins	17 glycoproteins				Fterm		glycoproteins			Among those identified, human milk has 17 glycoproteins, which are both O- and N-glycosylated, whereas caprine and bovine have 10 and 1, respectively.
35504880	1	16	gly	glycoproteins	234:246	arg1	many glycoproteins	many glycoproteins				Fterm		glycoproteins	34215		C1GalT1 is an essential inverting glycosyltransferase responsible for synthesizing the core 1 structure, a common precursor for mucin-type O-glycans found in many glycoproteins.
35504880	1	26	gly	found	220:224	arg1	many glycoproteins AND mucin-type O-glycans	many glycoproteins			mucin-type O-glycans	Fterm		glycoproteins	34215		C1GalT1 is an essential inverting glycosyltransferase responsible for synthesizing the core 1 structure, a common precursor for mucin-type O-glycans found in many glycoproteins.
31657490	4	18	gly	glycopeptide	708:719	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	The obtained material was used for glycopeptide enrichment.
31657490	1	7	gly	Glycosylation	131:143	arg1	proteins	proteins				Fterm		proteins			RATIONALE Glycosylation of proteins plays an important role in life activities, but the concentration of naturally occurring glycopeptides is usually relatively low, and glycosylation has microfacies heterogeneity, so direct mass spectrometry is not feasible.
31657490	1	24	gly	glycopeptides	246:258	arg2	naturally occurring glycopeptides			naturally occurring glycopeptides						glycopeptides	RATIONALE Glycosylation of proteins plays an important role in life activities, but the concentration of naturally occurring glycopeptides is usually relatively low, and glycosylation has microfacies heterogeneity, so direct mass spectrometry is not feasible.
35091091	2	78	gly	glycopeptides	421:433	arg2	glycopeptides			glycopeptides						glycopeptides	We recently introduced a fast and sensitive glycoproteomics search method in our MSFragger search engine, which reports glycopeptides as a combination of a peptide sequence and the mass of the attached glycan.
36124803	4	22	gly	glycoprotein	687:698	arg1	our glycoprotein database	our glycoprotein database				Fterm		glycoprotein			We show how to enhance site annotation with federated queries involving UniProt and GlyConnect, our glycoprotein database.
35766466	9	14	gly	nonsialylated	1630:1642	arg1	nonsialylated N-glycans				nonsialylated N-glycans						Sialidase utilization in MALDI-IMS dramatically increases sensitivity and increases on-tissue endoglycosidase efficiency, making it a very useful companion technique to specifically detect nonsialylated N-glycans.
36192063	8	75	gly	sialylated	1535:1544	arg1	307 sialylated intact N-glycopeptides			307 sialylated intact N-glycopeptides						N-glycopeptides	In total, 307 sialylated intact N-glycopeptides with linkage-specific sialic acid residues were identified together with 29 N-glycans with α2,6-linked sialic acids and 55 N-glycans with α2,3-linked sialic acids.
36192063	8	78	gly	N-glycopeptides	1553:1567	arg2	307 sialylated intact N-glycopeptides			N-glycopeptides	linkage-specific sialic acid residues					N-glycopeptides	In total, 307 sialylated intact N-glycopeptides with linkage-specific sialic acid residues were identified together with 29 N-glycans with α2,6-linked sialic acids and 55 N-glycans with α2,3-linked sialic acids.
36399685	4	5	gly	N-glycoprotein	742:755	arg1	cell-free N-glycoprotein synthesis	cell-free N-glycoprotein synthesis				Fterm		N-glycoprotein			The ability to generate glycosylated ribosome-nascent chain (glycoRNC) complexes was enabled by integrating SecM-mediated translation arrest with methods for cell-free N-glycoprotein synthesis.
35370997	1	28	gly	glycoprotein	253:264	arg1	the most abundant glycoprotein	the most abundant glycoprotein				Fterm		glycoprotein			The N-glycome of immunoglobulin G (IgG), the most abundant glycoprotein in human blood serum, reflects pathological conditions of autoimmunity and is sensitive to medicines applied in disease therapy.
35370997	1	28	gly	glycoprotein	253:264	arg1	immunoglobulin G	immunoglobulin G				Cterm		immunoglobulin G			The N-glycome of immunoglobulin G (IgG), the most abundant glycoprotein in human blood serum, reflects pathological conditions of autoimmunity and is sensitive to medicines applied in disease therapy.
32796070	10	26	gly	glycoprotein	1694:1705	arg1	the HIV-1 glycoprotein conformation	the HIV-1 glycoprotein conformation				Fterm		glycoprotein			Defining the specific effects of SERINC5 on the HIV-1 glycoprotein conformation may be useful for designing new antiviral strategies targeting Env.
35717753	4	4	gly	L-His	563:567	arg1	(Cu2+)(A)(L-His)-H]+ and the mononuclear copper-bound quaternary ions [(Cu2+)(A)(L-Ser)3-H]+ (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine			His	(Cu2+)(A)(L-His)-H]+ and the mononuclear copper-bound quaternary ions [(Cu2+)(A)(L-Ser)3-H]+ (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine					His	In this study, two novel complex ions, the mononuclear copper-bound dimeric ions [(Cu2+)(A)(L-His)-H]+ and the mononuclear copper-bound quaternary ions [(Cu2+)(A)(L-Ser)3-H]+ (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine), were designed for the collision-induced dissociation-based identification and relative quantification of 14 disaccharide isomers.
36825496	2	2	gly	fucosylated	181:191	arg1	fucosylated glycans				fucosylated glycans						A main obstacle to the sequencing of fucosylated glycans is the migration of fucose, which leads to misinterpretations in mass spectrometry analysis.
36813234	0	11	gly	P-glycoprotein	74:87	arg1	P-glycoprotein expression	P-glycoprotein expression				Fterm		P-glycoprotein			Expression of GnT-III decreases chemoresistance via negatively regulating P-glycoprotein expression: Involvement of the TNFR2-NF-κB signaling pathway.
35945033	2	43	gly	asparagine-linked	609:625	arg1	N-glycans			asparagine	N-glycans					asparagine	The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35945033	2	43	gly	asparagine-linked	609:625	arg1	asparagine-linked oligosaccharides			asparagine	asparagine-linked oligosaccharides					asparagine	The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35945033	2	129	gly	glycoproteins	661:673	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35945033	2	129	gly	glycoproteins	661:673	arg1	asparagine-linked oligosaccharides	glycoproteins			asparagine-linked oligosaccharides	Fterm		glycoproteins			The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35248671	1	34	gly	glycoproteins	137:149	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			BACKGROUND N-Glycan branching regulates various functions of glycoproteins.
32357974	5	70	gly	glycosylation	939:951	arg2	the N-linked glycosylation motif NX(S/T)			the N-linked glycosylation motif NX(S/T)						motif	Notably, we found that the N-linked glycosylation motif NX(S/T) was highly abundant across asparagine-deamidated HLA-bound peptides.
36478308	5	34	gly	glycosylation	823:835	arg2	isolated glycosylation sites			isolated glycosylation sites						sites	Three different protease digests were utilised to generate peptides with isolated glycosylation sites.
37266972	13	73	gly	deglycosylated	1655:1668	arg1	deglycosylated pharmaceutical proteins	deglycosylated pharmaceutical proteins				Fterm		proteins			Production of deglycosylated pharmaceutical proteins in BY-2 cells expands the set of glycoengineered BY-2 cell lines.
35343965	5	41	gly	glycoproteins	826:838	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			However, despite the advances made in the understanding of glycan composition, structure, and biosynthesis pathways, understanding of the role of glycoproteins in pathogenicity or interaction with the environment remains very limited.
36380917	0	13	gly	sialylation	62:72	arg1	glucagon-like peptide 1			glucagon-like peptide 1						peptide	Identification of the effect of N-glycan modification and its sialylation on proteolytic stability and glucose-stabilizing activity of glucagon-like peptide 1 by site-directed enzymatic glycosylation.
36380917	0	40	gly	peptide	149:155	arg1	glucose-stabilizing activity			peptide	glucose-stabilizing activity					peptide	Identification of the effect of N-glycan modification and its sialylation on proteolytic stability and glucose-stabilizing activity of glucagon-like peptide 1 by site-directed enzymatic glycosylation.
35943155	11	77	gly	glycoprotein	1843:1854	arg1	human α-1 acid glycoprotein	human α-1 acid glycoprotein				Fterm		glycoprotein			Fuc18, Fuc19A, and Fuc39 also removed l-fucose from neoglycoproteins and human α-1 acid glycoprotein.
35943155	11	130	gly	neoglycoproteins	1807:1822	arg1	neoglycoproteins	neoglycoproteins				Fterm		neoglycoproteins			Fuc18, Fuc19A, and Fuc39 also removed l-fucose from neoglycoproteins and human α-1 acid glycoprotein.
35104505	8	11	gly	glycoproteins	1511:1523	arg1	substrate glycoproteins	substrate glycoproteins				Fterm		glycoproteins			Finally, docking models of GnT-V with substrate glycoproteins suggested that the N domain could interact with the substrate polypeptide directly.
36329887	11	73	gly	glycoforms	2172:2181	arg1	FSH glycoforms	FSH glycoforms				OGER		FSH			Selective αAsn<sup>52</sup> deglycosylation of representative pituitary hFSH glycoform Superdex 75 gel filtration fractions followed by ESI-IM-CID mass spectrometry revealed tri-antennary glycans predominated even in the lowest molecular weight FSH glycoforms.
35670884	6	12	gly	P261	1084:1087	arg1	residues			residues						residues	Molecular dynamics analysis revealed that core fucosylation of SPARC-induced dynamic conformational changes in its N-glycan, allowing terminal galactose and N-acetylglucosamine to interact with K150, P261 and H264 residues, thereby promoting collagen binding.
32365408	8	8	gly	OGT	1375:1377	arg1	5SGlcNHex	OGT			5SGlcNHex	OGER		OGT	Q8CGY8		Moreover, treatment of cells with the OGT inhibitor, 5SGlcNHex, increases the level of uptake of α-syn PFFs, further supporting O-GlcNAcylation of proteins driving these effects.
34778211	5	115	gly	glycoprotein	777:788	arg1	host cell glycoprotein impurities	host cell glycoprotein impurities				Fterm		glycoprotein			The characterization of monoclonal antibodies as well as host cell glycoprotein impurities are required for quality control purposes under regulation rules.
36482940	6	47	gly	N-glycoprotein	1491:1504	arg1	putative N-glycoprotein biomarker candidates	putative N-glycoprotein biomarker candidates				Fterm		N-glycoprotein			This discovery study provides putative N-glycoprotein biomarker candidates for future validation study using multiple reaction monitoring and biochemical methods.
37211816	2	29	gly	N-glycosylation	473:487	arg2	an N-glycosylation N-X-T sequon			an N-glycosylation N-X-T sequon						sequon	To further increase the versatility of VHHs, we explored the VHH scaffold in a structure-guided approach to select regions where the introduction of an N-glycosylation N-X-T sequon and its associated glycan should not interfere with protein folding or epitope recognition.
37162352	7	39	gly	glycosylated	966:977	arg1	The glycosylated sites			The glycosylated sites						sites	The glycosylated sites were predominantly located in the periplasm, where they are postulated to be beneficial for protein folding/stability.
33078708	3	8	gly	glycosylated	511:522	arg1	CatSper1	CatSper1				OGER		CatSper1	Q8NEC5		Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca2+ and phosphorylation cascades.
33078708	3	8	gly	glycosylated	511:522	arg1	an O-linked glycosylated protein	an O-linked glycosylated protein				Fterm		protein			Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca2+ and phosphorylation cascades.
35077806	3	12	gly	glycoproteins	547:559	arg1	their homing secreted glycoproteins	their homing secreted glycoproteins				Fterm		glycoproteins			Despite the numerous studies aiming at the biochemical characterization of anuran skin secretions, little is known about protein-linked oligosaccharides, their synthesis pathways, and their homing secreted glycoproteins.
35507105	8	63	gly	glycoforms	1607:1616	arg1	insulin glycoforms	insulin glycoforms				PUBTATOR		insulin	3630		This work helps better explain how O-linked glycosylation influences the proteolytic stability and monomeric propensity of insulin, illuminating a path towards rational molecular design of insulin glycoforms.
36660462	6	20	gly	glycopeptides	1057:1069	arg2	123 differently expressed glycopeptides			123 differently expressed glycopeptides						glycopeptides	A total of 123 differently expressed glycopeptides, which were from 47 glycoproteins were identified among three groups.
36660462	6	23	gly	glycoproteins	1091:1103	arg1	47 glycoproteins	47 glycoproteins				Fterm		glycoproteins			A total of 123 differently expressed glycopeptides, which were from 47 glycoproteins were identified among three groups.
37148536	10	133	gly	EPO-Fc sialylation	1829:1846	arg1	tri-				tri-						Inhibitor supplementation also led to a decrease in bi-, tri-, and tetra-antennary structures and up to 50% lower EPO-Fc sialylation.
37148536	10	133	gly	EPO-Fc sialylation	1829:1846	arg1	bi-				bi-						Inhibitor supplementation also led to a decrease in bi-, tri-, and tetra-antennary structures and up to 50% lower EPO-Fc sialylation.
37148536	10	133	gly	EPO-Fc sialylation	1829:1846	arg1	tetra-antennary structures				tetra-antennary structures						Inhibitor supplementation also led to a decrease in bi-, tri-, and tetra-antennary structures and up to 50% lower EPO-Fc sialylation.
32169063	4	7	gly	bunit	662:666	arg1	sequences			sequences						sequences	RESULTS Two novel long-acting recombinant human IFN-α2b (rhIFN-α2b) proteins were designed in which the carboxyl-terminal peptide (CTP) of the human chorionic gonadotropin β su bunit and N-linked glycosylation sequences were linked to rhIFN-α2b.
36935145	2	15	gly	N-glycosylation	696:710	arg2	the Asn residue			the Asn residue						residue	For the modification site, N-glycosylation occurs on the Asn residue among the consensus N-X-S/T/C (X≠P) motif; mutation-originated amino acid change may lead to loss of such an original motif and thus loss-of-glycosylation (LoG) or gain of such a new motif and thus gain-of-glycosylation (GoG).
37354227	2	51	gly	glycosylated	287:298	arg1	Viral proteins	Viral proteins				Fterm		proteins			Viral proteins used in cell entry are often extensively glycosylated to assist in protein folding, provide stability, and shield the virus from immune recognition by its host (described as a "glycan shield").
32357974	9	11	gly	deglycosylation	1819:1833	arg1	formerly glycosylated proteins	formerly glycosylated proteins				Fterm		proteins			This indicates that a large proportion of HLA-I bound asparagine deamidated peptides are generated from formerly glycosylated proteins that have undergone deglycosylation via the ER-associated protein degradation (ERAD) pathway.
32357974	9	60	gly	glycosylated	1777:1788	arg1	formerly glycosylated proteins	formerly glycosylated proteins				Fterm		proteins			This indicates that a large proportion of HLA-I bound asparagine deamidated peptides are generated from formerly glycosylated proteins that have undergone deglycosylation via the ER-associated protein degradation (ERAD) pathway.
36637420	5	11	gly	N-glycosylation	918:932	arg2	two putative N-glycosylation sites	HAI-2		sites, Asn-57 and Asn-94				HAI-2	10653	sites, Asn-57 and Asn-94	HAI-2 contains two putative N-glycosylation sites, Asn-57 and Asn-94, point mutations of which were generated and characterized in this study.
36637420	5	11	gly	N-glycosylation	918:932	arg2	Asn-94	HAI-2		sites, Asn-57 and Asn-94				HAI-2	10653	sites, Asn-57 and Asn-94	HAI-2 contains two putative N-glycosylation sites, Asn-57 and Asn-94, point mutations of which were generated and characterized in this study.
36637420	5	11	gly	N-glycosylation	918:932	arg2	Asn-94	HAI-2		sites, Asn-57 and Asn-94				HAI-2	10653	sites, Asn-57 and Asn-94	HAI-2 contains two putative N-glycosylation sites, Asn-57 and Asn-94, point mutations of which were generated and characterized in this study.
36542493	5	3	gly	glycopeptides	641:653	arg2	glycopeptides			glycopeptides						glycopeptides	Using glycopeptides as haptens, we generated first-in-class poly- and monoclonal antibodies, termed Anti-SerHep1a and Anti-SerHep1b, that stereoselectively recognize Ser O-heptosylation (d/l-glycero) with high specificity in vitro and in vivo.
36329887	5	72	gly	attached	878:885	arg1	FSHα Asn<sup>52</sup> AND a single biantennary glycan			FSHα Asn<sup>52</sup>	a single biantennary glycan					Asn	It was noted that a single biantennary glycan attached to FSHα Asn<sup>52</sup> might preemptively fill the central pocket in this complex and prevent the other two FSH ligands from binding the remaining ligand-binding sites.
36388954	2	9	gly	N-glycosylation	296:310	arg2	five distinct N-glycosylation sites			five distinct N-glycosylation sites						sites	We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome.
36388954	2	45	gly	protein	269:275	arg1	intracellular, site-specific glycan processing	protein			intracellular, site-specific glycan processing	Fterm		protein			We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome.
37271268	5	43	gly	contained	811:819	arg1	LNP-2 AND an additional 1,4-Glc glycosidic linkage	LNP-2			an additional 1,4-Glc glycosidic linkage	OGER		LNP	Q9C0E8		Additionally, LNP-2 contained an additional 1,4-Glc glycosidic linkage in comparison to LNP-1.
34650217	8	23	gly	KAT5	1288:1291	arg1	targeting HBP-mediated O-GlcNAcylation	KAT5			targeting HBP-mediated O-GlcNAcylation	OGER		KAT5	Q8CHK4		In addition, targeting HBP-mediated O-GlcNAcylation of KAT5 inhibits lung metastasis of HCC in hepatospecific Pck1-deletion mice.
32820167	6	29	gly	O-glycosites	961:972	arg2	O-glycosites			O-glycosites						O-glycosites	Our study highlights the importance of O-glycosylation in the biology of peptide hormones, and our map of O-glycosites in this large class of biomolecules serves as a discovery platform for an important class of molecules with potential opportunities for drug designs.
36565355	2	49	gly	domain	234:239	arg1	36 tandem epidermal growth factor-like (EGF) repeats			domain	36 tandem epidermal growth factor-like (EGF) repeats					domain	The receptor contains an extracellular domain (ECD) with 36 tandem epidermal growth factor-like (EGF) repeats.
36478308	6	11	gly	glycopeptides	848:860	arg2	The glycopeptides			The glycopeptides						glycopeptides	The glycopeptides were then analysed using a Waters Q-TOF while using a data dependent acquisition (DDA) mass spectrometry experiment.
36598201	6	43	gly	glycosylation	820:832	arg2	both glycosylation sites			both glycosylation sites						sites	However, a recombinant virus that lost both glycosylation sites (glycosylation-defective virus) showed attenuated replication in cultured cell lines.
36192063	9	12	gly	α2,6-sialylation	1761:1776	arg1	Intact N-glycoproteins	Intact N-glycoproteins				Fterm		N-glycoproteins			Intact N-glycoproteins with α2,6-sialylation were associated with coronavirus disease-(COVID)-19.
36192063	9	113	gly	N-glycoproteins	1740:1754	arg1	Intact N-glycoproteins	N-glycoproteins			α2,6-sialylation	Fterm		N-glycoproteins			Intact N-glycoproteins with α2,6-sialylation were associated with coronavirus disease-(COVID)-19.
36698045	13	64	gly	glycosite	1778:1786	arg2	glycosite			glycosite						glycosite	Moreover, all of the analyses were performed on three consecutive manufactured batches and the glycosylation results on both glycosite and glycans showed good batch-to-batch consistency.
35247653	4	55	gly	sialylated	748:757	arg1	low sialylated glycans				low sialylated glycans						A superior amount of low sialylated glycans and less branched structures were detected in NeuroEPO plus compare to other commercial rhEPOs.
35495330	3	60	gly	dimannosylated	944:957	arg1	dimannosylated peptide			dimannosylated peptide						peptide	Cys-Cys mutagenesis with ion-pairing amino-acids glutamic acid and arginine was calculated by in silico structure-based protein design and allowed for recognizing dimannose and dimannosylated peptide binding to low-affinity binding sites (K D ≈ 11 μM for one C58-C73 bond, and binding to dimannosylated peptide).
36036581	4	18	gly	glycosites	812:821	arg2	20 glycosites			20 glycosites						glycosites	In addition to the common N-glycans as detected in previous studies, many uncommon glycosylation structures such as LacdiNAc structures, Lewis structures, Mannose 6-phosphate (M6P) residues, and bisected core structures were unambiguously mapped at a total of 20 glycosites in the S protein trimer and protomer.
36838941	1	53	gly	used	226:229	arg2	bio-based hydrogel composites			bio-based hydrogel composites						composites	In this work, bio-based hydrogel composites of xanthan gum and cellulose fibers were developed to be used both as soil conditioners and topsoil covers, to promote plant growth and forest protection.
32149134	1	16	gly	glycoprotein	145:156	arg1	a type I transmembrane glycoprotein	a type I transmembrane glycoprotein				Fterm		glycoprotein			PURPOSE CD147, also known as BSG, is a type I transmembrane glycoprotein that belonged to immunoglobulin superfamily.
32149134	1	16	gly	glycoprotein	145:156	arg1	PURPOSE CD147	PURPOSE CD147				PUBTATOR		|PURPOSE
CD147|	682		PURPOSE CD147, also known as BSG, is a type I transmembrane glycoprotein that belonged to immunoglobulin superfamily.
32149134	2	29	gly	glycosylated	231:242	arg1	Mature CD147	Mature CD147				PUBTATOR		Mature CD147	682		Mature CD147 is an N-linked glycosylated protein and exists on the transmembrane and as soluble forms in tumors.
32149134	2	29	gly	glycosylated	231:242	arg1	an N-linked glycosylated protein	an N-linked glycosylated protein				Fterm		protein			Mature CD147 is an N-linked glycosylated protein and exists on the transmembrane and as soluble forms in tumors.
35104505	9	13	gly	glycoprotein	1701:1712	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			Our findings suggest that the N domain of GnT-V plays a critical role in the recognition of glycoprotein substrates, providing new insights into the mechanism of substrate-selective biosynthesis of N-glycans.
32130226	5	11	gly	glycosylated	947:958	arg1	an OST-dependent glycosylated protein	an OST-dependent glycosylated protein				Fterm		protein			We identified vitellogenin VIT-6 as an OST-dependent glycosylated protein, critical for maintaining survival on PA14.
32130226	5	11	gly	glycosylated	947:958	arg1	vitellogenin VIT-6	vitellogenin VIT-6				OGER		VIT	Q6UXI7		We identified vitellogenin VIT-6 as an OST-dependent glycosylated protein, critical for maintaining survival on PA14.
35307819	5	61	gly	IgGs	818:821	arg1	the glycans composition	IgGs			the glycans composition	Cterm		IgGs	668542		We found that SARS-CoV-2+ individuals display, at diagnosis, variations in the glycans composition of circulating IgGs.
36598201	0	101	gly	N-Glycosylation	0:14	arg1	Rotavirus NSP4 Protein	Rotavirus NSP4 Protein				OGER		Rotavirus NSP4 Protein	Q14B24		N-Glycosylation of Rotavirus NSP4 Protein Affects Viral Replication and Pathogenesis.
35481895	5	29	gly	identified	908:917	arg1	a trisaccharide α-GlcNAc-4-β-GlcNAc3NGaAN-4-β-Glc-Asn			a trisaccharide α-GlcNAc-4-β-GlcNAc3NGaAN-4-β-Glc-Asn						Asn	Two N-glycans were identified by NMR and MS analysis: a trisaccharide α-GlcNAc-4-β-GlcNAc3NGaAN-4-β-Glc-Asn where the second residue is 2-N-acetyl, 3-N-glyceryl-glucosamide and a disaccharide β-GlcNAc3NAcAN-4-β-Glc-Asn, where the terminal residue is 2,3 di-N-acetyl-glucosamide.
35311852	9	87	gly	glycoproteins	1365:1377	arg1	κ-casein	κ-casein				PUBTATOR		-casein	281728		Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	87	gly	glycoproteins	1365:1377	arg1	bovine fetuin	bovine fetuin				Fterm		fetuin			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	87	gly	glycoproteins	1365:1377	arg1	ribonuclease B	ribonuclease B				Cterm		ribonuclease B			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	87	gly	glycoproteins	1365:1377	arg1	model glycoproteins	model glycoproteins				Fterm		glycoproteins			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	97	gly	derived	1346:1352	arg1	bovine fetuin AND N- and O-glycan isomers	bovine fetuin			N- and O-glycan isomers	Fterm		fetuin			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	97	gly	derived	1346:1352	arg1	ribonuclease B AND N- and O-glycan isomers	ribonuclease B			N- and O-glycan isomers	Cterm		ribonuclease B			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	97	gly	derived	1346:1352	arg1	model glycoproteins AND N- and O-glycan isomers	model glycoproteins			N- and O-glycan isomers	Fterm		glycoproteins			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	97	gly	derived	1346:1352	arg1	κ-casein AND N- and O-glycan isomers	κ-casein			N- and O-glycan isomers	PUBTATOR		-casein	281728		Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
36121379	8	48	gly	sialylated	1233:1242	arg1	highly sialylated multi-antennary glycans				highly sialylated multi-antennary glycans						HILIC separates the crude mixture of highly sialylated multi-antennary glycans, MS provides information on glycan composition, and IMS is used to distinguish and quantify α2,6- and α2,3-linked sialic acid isomers based on characteristic fragments.
37336068	9	70	gly	glycosylated	1657:1668	arg1	β-1,4-GlcNAc glycosylated WTAs				β-1,4-GlcNAc glycosylated WTAs						This study demonstrated that β-1,4-GlcNAc glycosylated WTAs are promising antigens for further development against MRSA.
35604098	2	23	gly	asparagine-linked	435:451	arg1	asparagine-linked (N-) glycans			asparagine	asparagine-linked (N-) glycans					asparagine	Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine-linked (N-) glycans in the organelles.
35604098	2	44	gly	glycoproteins	314:326	arg1	homogeneous glycoproteins	homogeneous glycoproteins				Fterm		glycoproteins			Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine-linked (N-) glycans in the organelles.
35594729	0	44	gly	oxidase	41:47	arg1	N-linked glycans	oxidase			N-linked glycans	Fterm		oxidase			Effects of N-linked glycans of bilirubin oxidase on direct electron transfer-type bioelectrocatalysis.
34878920	0	88	gly	Glycosylation	0:12	arg1	ALV-J Envelope Protein	ALV-J Envelope Protein				PUBTATOR		ALV-J Envelope Protein	64006		Glycosylation of ALV-J Envelope Protein at Sites 17 and 193 Is Pivotal in the Virus Infection.
36121379	7	6	gly	released	1135:1142	arg2	human alpha-1-acid glycoprotein AND the glycan structures	human alpha-1-acid glycoprotein			the glycan structures	Fterm		glycoprotein			Here, we close this gap and combine hydrophilic interaction liquid chromatography (HILIC) with IM-MS to analyze the glycan structures released from human alpha-1-acid glycoprotein (hAGP).
36121379	7	57	gly	glycoprotein	1168:1179	arg1	human alpha-1-acid glycoprotein	human alpha-1-acid glycoprotein				Fterm		glycoprotein			Here, we close this gap and combine hydrophilic interaction liquid chromatography (HILIC) with IM-MS to analyze the glycan structures released from human alpha-1-acid glycoprotein (hAGP).
36809652	1	85	gly	O-glycoprotein	209:222	arg1	a highly polymorphic O-glycoprotein	a highly polymorphic O-glycoprotein				Fterm		O-glycoprotein			Apolipoprotein(a) [apo(a)] is a highly polymorphic O-glycoprotein circulating in human plasma as lipoprotein(a) [Lp(a)].
36809652	1	85	gly	O-glycoprotein	209:222	arg1	Apolipoprotein(a) [apo	Apolipoprotein(a) [apo				OGER		apo(a	P08519		Apolipoprotein(a) [apo(a)] is a highly polymorphic O-glycoprotein circulating in human plasma as lipoprotein(a) [Lp(a)].
34960798	9	117	gly	glycoprotein	1601:1612	arg1	surface glycoprotein	surface glycoprotein				Fterm		glycoprotein			In contrast, aa sequences of surface glycoprotein exhibited higher variability confirming type-specific variation in the SU5 epitope.
37129482	7	25	gly	glycoproteins	1150:1162	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In addition to glycan structures, key information, such as glycan-related genes, relevant diseases, glycoproteins, and pathways were integrated and cross-linked with one another.
37294165	3	13	gly	glycosylated	528:539	arg1	glycosylated SPINK 13	glycosylated SPINK 13				OGER		SPINK 13	Q1W4C9		In addition to this, the preparation of glycosylated SPINK 13 has not been examined by both the cell expression method and chemical synthesis.
37219590	1	26	gly	glycoprotein	168:179	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			The oligosaccharides in N-glycosylation provide key structural and functional contributions to a glycoprotein.
34695439	5	87	gly	glycosylation	799:811	arg1	a protein	a protein				Fterm		protein			We generated B3GLCT knockout (KO) RPE cells and analyzed glycosylation and secretion of thrombospondin 1 (TSP1), a protein involved in cellular processes highly relevant to AMD.
34695439	5	87	gly	glycosylation	799:811	arg1	thrombospondin 1	thrombospondin 1				PUBTATOR		thrombospondin 1	7057		We generated B3GLCT knockout (KO) RPE cells and analyzed glycosylation and secretion of thrombospondin 1 (TSP1), a protein involved in cellular processes highly relevant to AMD.
34695439	5	87	gly	glycosylation	799:811	arg1	TSP1	TSP1				PUBTATOR		TSP1	7057		We generated B3GLCT knockout (KO) RPE cells and analyzed glycosylation and secretion of thrombospondin 1 (TSP1), a protein involved in cellular processes highly relevant to AMD.
34452516	5	72	gly	glycoprotein	1006:1017	arg1	both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis				both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis						Mutation of the LH6 initiating glycosyltransferase homolog, PglC, necessary for both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis, prevented infection by the lytic podovirus CAP1, while mutation of the pilin protein, PilA, prevented infection by CAP3, representing the lytic cystoviruses.
37162352	5	13	gly	sequence	716:723	arg1	the sequence Hex-(Me-dHex)-Me-HexA-Pent-HexA-Me-HexNAcA				the sequence Hex-(Me-dHex)-Me-HexA-Pent-HexA-Me-HexNAcA						The structure of the major glycan was found to be a hexasaccharide with the sequence Hex-(Me-dHex)-Me-HexA-Pent-HexA-Me-HexNAcA.
36809652	0	93	gly	structures	9:18	arg1	apo(a) subunit	subunit			structures	Fterm		subunit			O-glycan structures in apo(a) subunit of human lipoprotein(a) suppresses the pro-angiogenic activity of galectin-1 on human umbilical vein endothelial cells.
35276597	5	46	gly	N-glycosylation	1207:1221	arg1	rMIR				rMIR						The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone.
35276597	5	46	gly	N-glycosylation	1207:1221	arg1	nMIR				nMIR						The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone.
35276597	5	67	gly	peptide	1264:1270	arg1	the peptide backbone				the peptide backbone						The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone.
35987426	14	0	gly	glycoproteins	2206:2218	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			CONCLUSIONS This study showed that the glycan pattern becomes more complex from the basal to surface layer of the urothelium and that surface cells could modify the composition of urine via the secretion of glycoproteins.
36758646	2	32	gly	glycoproteins	384:396	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In insects, α-1,6-fucosyltransferase (FucT6) is an important enzyme in the glycosylation pathway, modifying the core structure of N-glycans on glycoproteins with the addition of a fucose residue.
35065968	7	16	gly	carrying	1502:1509	arg1	peptides AND a type A glycan modification			peptides	a type A glycan modification					peptides	In conclusion, we show that a common enrichment strategy results in reliable identification of peptides carrying a type A glycan modification, and that the results obtained can be used to advance models about its biosynthesis.
36377874	10	123	gly	glycosylated	1675:1686	arg1	The glycan loop deletion mutants				The glycan loop deletion mutants						The glycan loop deletion mutants are not glycosylated, which are attenuated in adult A129 mouse model and reduced oral infections in mosquitoes.
35247653	7	6	gly	glycoprotein	1301:1312	arg1	intact glycoprotein	intact glycoprotein				Fterm		glycoprotein			The proposed glycomic and intact glycoprotein methods provide a robust and reliable analytical platform for NeuroEPO plus characterization and for its future implementation as biopharmaceutical in neurodegenerative diseases.
36585837	6	9	gly	glycopeptides	815:827	arg2	Purified glycopeptides			Purified glycopeptides						glycopeptides	Purified glycopeptides were analysed by label-free liquid chromatography tandem mass-spectrometry (LC-MS/MS).
37069671	9	31	gly	composition	1404:1414	arg1	particular abundant storage polysaccharides			position	particular abundant storage polysaccharides					position	CONCLUSIONS We show that the amounts and composition of dissolved polysaccharides, in particular abundant storage polysaccharides, have a pronounced influence on the composition of abundant bacterioplankton members during phytoplankton blooms, some of which compete for similar polysaccharide niches.
36463509	4	28	gly	glycosylation	626:638	arg1	an Fc-fusion protein	an Fc-fusion protein				Fterm		protein			In this study, the biosimilars of a therapeutic monoclonal antibody and an Fc-fusion protein with moderate and heavy glycosylation, respectively, were employed as representative biopharmaceuticals for released glycan analysis using liquid chromatography-tandem mass spectrometry instead of conventional mass spectrometry-based analysis.
36210615	2	89	gly	glycoprotein	346:357	arg1	their glycoprotein content	their glycoprotein content				Fterm		glycoprotein			Studies by our group have shown that Bothrops venoms are distinctly defined by their glycoprotein content, and that most hybrid/complex N-glycans identified in these venoms contain sialic acid.
36669592	6	6	gly	sialylated	1039:1048	arg1	sialylated O-glycan structures				sialylated O-glycan structures						It was found that the fully differentiated butyrate-stimulated cells are characterized by a higher expression of sialylated O-glycan structures, whereas fucosylation is downregulated with differentiation.
35662639	4	52	gly	glycoproteins	1218:1230	arg1	sialic acid-containing glycoproteins				sialic acid-containing glycoproteins						To further investigate the mechanisms involved in the generation of different venoms by related snakes, here the glycoproteomes of nine Bothrops venoms (Bothrops atrox, B. cotiara, Bothrops erythromelas, Bothrops fonsecai, B. insularis, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni and Bothrops neuwiedi) were comparatively analyzed by enrichment with three lectins of different specificities, recognizing bisecting N-acetylglucosamine- and sialic acid-containing glycoproteins, and mass spectrometry.
36136114	4	33	gly	glycosylation	568:580	arg1	the active peptide			peptide						peptide	Fc-fusion proteins can be modified by complex glycosylation on the active peptide, the fragment crystallizable (Fc) domain, and the linker peptides.
36136114	4	33	gly	glycosylation	568:580	arg1	the linker peptides			peptides						peptides	Fc-fusion proteins can be modified by complex glycosylation on the active peptide, the fragment crystallizable (Fc) domain, and the linker peptides.
36136114	4	33	gly	glycosylation	568:580	arg1	the fragment crystallizable (Fc) domain			domain						domain	Fc-fusion proteins can be modified by complex glycosylation on the active peptide, the fragment crystallizable (Fc) domain, and the linker peptides.
35316032	3	28	gly	deglycosylation	820:834	arg1	an M6P-glycan				an M6P-glycan						Using trastuzumab and cetuximab as model antibodies, we showed that the wild-type endoglycosidase S (Endo-S) could efficiently perform the antibody deglycosylation and simultaneous transfer of an M6P-glycan from a synthetic M6P-glycan oxazoline to the deglycosylated antibody in a one-pot manner, giving structurally well-defined antibody-M6P glycan conjugates.
35092134	11	13	gly	MET	1623:1625	arg1	N-glycans			MET	N-glycans					MET	The overall findings suggest that N-glycans of MET affect the status and the function of the receptor in a site-specific manner.
35943155	18	82	gly	glycoproteins	3061:3073	arg1	naturally occurring glycoproteins	naturally occurring glycoproteins				Fterm		glycoproteins			These enzymes showed different substrate specificities toward HMOs, HBGAs, naturally occurring glycoproteins, and neoglycoproteins.
35943155	18	157	gly	neoglycoproteins	3080:3095	arg1	neoglycoproteins	neoglycoproteins				Fterm		neoglycoproteins			These enzymes showed different substrate specificities toward HMOs, HBGAs, naturally occurring glycoproteins, and neoglycoproteins.
34379775	2	35	gly	O-glycosylated	425:438	arg1	O-glycosylated proteins	O-glycosylated proteins				Fterm		proteins			The function of cervical mucus is critically linked to its rheological properties that are in turn dictated by O-glycosylated proteins, called mucins.
33073321	0	97	gly	glycopeptides	32:44	arg2	urinary N-linked glycopeptides			urinary N-linked glycopeptides						glycopeptides	Recognition of urinary N-linked glycopeptides in kidney cancer patients by hydrophilic carbohydrate functionalized magnetic metal organic framework combined with LC-MS/MS.
37294165	8	15	gly	glycoprotein	1470:1481	arg1	a target glycoprotein	a target glycoprotein				Fterm		glycoprotein			This synthetic concept enables us to repetitively synthesize a target glycoprotein easily.
34399822	2	103	gly	kinase	415:420	arg1	O-GlcNAcylation	kinase 3			O-GlcNAcylation	Fterm		kinase 3			The O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) of necroptotic signal molecule receptor-interacting serine-threonine kinase 3 (RIPK3) was reported to exert a protective effect in gut inflammation.
36740532	7	28	gly	O-glycosites	1140:1151	arg2	62 unique O-glycosites			62 unique O-glycosites						O-glycosites	In the analysis of the recombinant protein, we identified 62 unique O-glycosites.
36373229	7	6	gly	O-glycopeptide	1567:1580	arg2	O-glycopeptide			O-glycopeptide						O-glycopeptide	These putative motifs can be further used to define new protease cleavage settings that lower search times and improve O-glycopeptide identifications.
36373229	7	41	gly	used	1485:1488	arg2	These putative motifs			These putative motifs						motifs	These putative motifs can be further used to define new protease cleavage settings that lower search times and improve O-glycopeptide identifications.
34939082	4	39	gly	glycopeptides	561:573	arg2	glycopeptides			glycopeptides						glycopeptides	We illustrate this method by showing visual representations of the glycans and glycopeptides from a variety of species.
36018613	13	71	gly	glycopeptide	1627:1638	arg2	glycopeptide sequence identification			glycopeptide sequence identification						glycopeptide	If general, the change to a furanose m/z 204 product ion structure fundamentally alters the ion population available for MS3 dissociation and glycopeptide sequence identification.
35802832	5	43	gly	disialylated	986:997	arg1	disialylated O-glycans				disialylated O-glycans						Bovine glycomacropeptide glycosylation promoted the immune activity of RAW264.7 cells, which may be linked to a higher content of disialylated O-glycans.
35320529	7	0	gly	N-glycosylation	1124:1138	arg2	five N-glycosylation sites			five N-glycosylation sites						sites	NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for endogenous galectins.
35320529	7	62	gly	modified	1177:1184	arg3	the five sites AND N-glycans			the five sites	N-glycans					sites	NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for endogenous galectins.
35320529	7	36	gly	glycosylated	1103:1114	arg2	five N-glycosylation sites	CD16a		sites		PUBTATOR		CD16a	2214	sites	NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for endogenous galectins.
35854001	1	44	gly	glycoprotein	231:242	arg1	glycoprotein functions	glycoprotein functions				Fterm		glycoprotein	269181		N-Glycosylation is a common post-translational modification, and the number of GlcNAc branches in N-glycans impacts glycoprotein functions.
32602701	4	28	gly	glycosylation	569:581	arg2	all four potential Asn glycosylation sites			all four potential Asn glycosylation sites						sites	Abolishing N-linked glycosylation by tunicamycin, glucosamine supplementation, or glutamine substitutions of all four potential Asn glycosylation sites blocked myonectin secretion.
32602701	4	81	gly	glycosylation	457:469	arg1	all four potential Asn glycosylation sites			all four potential Asn glycosylation sites						sites	Abolishing N-linked glycosylation by tunicamycin, glucosamine supplementation, or glutamine substitutions of all four potential Asn glycosylation sites blocked myonectin secretion.
36399685	7	44	gly	N-glycoproteins	1195:1209	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			We anticipate that our method will enable selection and evolution of N-glycoproteins with advantageous biological and biophysical properties.
34939126	8	25	gly	glycoproteins	1341:1353	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This study expands structural knowledge on fungal O-glycosylation and presents a ready-to-use enzymatic approach for controlled O-glycan engineering in glycoproteins expressed in filamentous fungi.
36192063	10	73	gly	N-glycoproteins	2047:2061	arg1	intact N-glycoproteins	intact N-glycoproteins				Fterm		N-glycoproteins			Additionally, many types of N-glycosylation including terminal N-galactosylation, core and/or branch fucosylation, α2,6-sialylation and terminal bisecting N-acetylglucosamine were identified and quantified in intact N-glycoproteins from immunoglobulin family.
36985724	3	34	gly	glycoproteins	504:516	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycoprotein in vitro N-glycan engineering using purified recombinant enzymes is an attractive strategy to produce glycoproteins with homogeneous N-glycoforms to elucidate the specific functions of N-glycans and develop better glycoprotein therapeutics.
36985724	3	41	gly	glycoprotein	616:627	arg1	better glycoprotein therapeutics	better glycoprotein therapeutics				Fterm		glycoprotein			Glycoprotein in vitro N-glycan engineering using purified recombinant enzymes is an attractive strategy to produce glycoproteins with homogeneous N-glycoforms to elucidate the specific functions of N-glycans and develop better glycoprotein therapeutics.
37156312	2	39	gly	O-glycosites	402:413	arg2	O-glycosites			O-glycosites						O-glycosites	Unlike N-glycosylation, the O-glycosylation has nonspecific sequence features and nonstable glycan core structure, which makes identification of O-glycosites more challenging either by experimental or computational methods.
36435935	3	30	gly	glycoprotein	493:504	arg1	glycoprotein biosynthesis	glycoprotein biosynthesis				Fterm		glycoprotein	281499		Precise recognition of the fully assembled glycan by OST is essential for the subsequent quality control steps of glycoprotein biosynthesis.
31941975	5	13	gly	N-glycopeptides	890:904	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Training and test sets of more than 800 MS/MS spectra of N-glycopeptides from the immunoglobulin gamma and alpha 1-acid-glycoprotein standards were selected for classification of the fucosylation types using supervised learning models.
31941975	5	75	gly	1-acid-glycoprotein	946:964	arg1	the immunoglobulin gamma and alpha 1-acid-glycoprotein standards	the immunoglobulin gamma and alpha 1-acid-glycoprotein standards				Fterm		1-acid-glycoprotein			Training and test sets of more than 800 MS/MS spectra of N-glycopeptides from the immunoglobulin gamma and alpha 1-acid-glycoprotein standards were selected for classification of the fucosylation types using supervised learning models.
34712242	4	16	gly	sialylated	798:807	arg1	sialylated glycan species				sialylated glycan species						While the position of the Fc glycan is conserved in macaques, differences in the frequency of glycoforms and the use of an alternate monosaccharide in sialylated glycan species add a degree of uncertainty to the testing of glycoengineered human antibodies in rhesus macaques.
36288283	4	15	gly	glycoprotein	622:633	arg1	the LASV glycoprotein complex	the LASV glycoprotein complex				Fterm		glycoprotein			Here, we describe biochemical analyses and high-resolution cryo-electron microscopy structures of a therapeutic cocktail of three broadly protective antibodies that target the LASV glycoprotein complex (GPC), previously identified from survivors of multiple LASV infections.
32357974	6	84	gly	N-glycosylated	1191:1204	arg1	nascently N-glycosylated polypeptides			nascently N-glycosylated polypeptides						polypeptides	This finding, demonstrated previously for a handful of deamidated T cell epitopes, implicates a more global role for the retrograde transport of nascently N-glycosylated polypeptides from the ER and their subsequent degradation within the cytosol to form HLA-ligand precursors.
36244450	6	7	gly	glycosylation	939:951	arg2	glycosylation site characterization			glycosylation site characterization						site	We conducted site-specific N- and O-glycosylation analysis and glycosylation site characterization using mass spectrometry by which several novel O-glycosylation sites were identified in mouse MR and confirmed in human full-length MR. This information guided experiments evaluating the receptor functionality by glycan microarray analysis in combination with glycan-modifying enzymes.
36244450	6	88	gly	O-glycosylation	1022:1036	arg2	several novel O-glycosylation sites			several novel O-glycosylation sites						sites	We conducted site-specific N- and O-glycosylation analysis and glycosylation site characterization using mass spectrometry by which several novel O-glycosylation sites were identified in mouse MR and confirmed in human full-length MR. This information guided experiments evaluating the receptor functionality by glycan microarray analysis in combination with glycan-modifying enzymes.
35065968	0	21	gly	C	105:105	arg1	A glycan modification	flagellin C			A glycan modification	PUBTATOR		flagellin C	4916757		New insights into the type A glycan modification of Clostridioides difficile flagellar protein flagellin C by phosphoproteomics analysis.
36096000	1	48	gly	glycosylation	117:129	arg1	egg white proteins	egg white proteins				Fterm		proteins			The glycosylation of egg white proteins (EWP) with maltodextrin (MD) was investigated by monitoring their gel properties and protein structure.
32839225	5	56	gly	sialylated	902:911	arg1	their sialylated oligosaccharides				their sialylated oligosaccharides						Using tandem MS coupled with liquid chromatography (LC-MS/MS), we found 142 nonreductant peptides, with 9 of them showing potential modifications on their sialylated oligosaccharides during capacitation.
36990248	4	17	gly	attached	816:823	arg1	secreted proteins AND the O-glycan	secreted proteins			the O-glycan	Fterm		proteins			We found GalNAc or GlcNAc monosaccharide and core 1 disaccharide (Galβ1-3-GalNAcα1-Ser/Thr) were major components of the O-glycan attached to secreted proteins produced in silkworms.
35773089	8	145	gly	N-acetylglucosamine	1654:1672	arg1	IgG	IgG			N-acetylglucosamine	PUBTATOR		IgG	668542		The most statistically significant changes included increased agalactosylation of IgG (meta-analysis 95% CI [0.03, 0.07], adjusted meta-analysis P= <0.0001), which regulates proinflammatory actions of IgG via complement system activation and indirectly as a lack of sialylation and decreased presence of bisecting N-acetylglucosamine on IgG (meta-analysis 95% CI [-0.11, -0.08], adjusted meta-analysis P= <0.0001), which indirectly affects antibody-dependent cell-mediated cytotoxicity.
32839225	13	12	gly	sialylation	2181:2191	arg1	ACO2	ACO2				PUBTATOR		ACO2	11429		These findings suggest that the switch from oxidative phosphorylation, over to glycolysis that occurs during capacitation may come about through sialylation of ACO2.
32839225	13	33	gly	ACO2	2196:2199	arg1	sialylation	ACO2			sialylation	PUBTATOR		ACO2	11429		These findings suggest that the switch from oxidative phosphorylation, over to glycolysis that occurs during capacitation may come about through sialylation of ACO2.
35279850	2	39	gly	N-glycans	403:411	arg1	transferrin	transferrin			N-glycans	OGER		transferrin	P02787		The biochemical hallmark of CDG-I is a partial absence of complete N-glycans on transferrin.
35447118	4	16	gly	fucosylation	871:882	arg1	typical lung-type N-glycans				typical lung-type N-glycans						We found that the N-glycans of SCLC-sEVs were characterized by the presence of structural units also found in the brain N-glycome, while NSCLC-sEVs were dominated by typical lung-type N-glycans with NSCLC-associated core fucosylation.
35882856	4	36	gly	fucosylated	657:667	arg1	fucosylated glycans				fucosylated glycans						Interestingly, TMPRSS2 and fucosylated glycans can mediate the binding/entry of TcsH independently, thus serving as redundant receptors.
33343565	3	24	gly	epitope	758:764	arg1	a self-binding epitope tag				a self-binding epitope tag						Complement protein C1q binding of the IgG fusions was enhanced by: 1) antigen fusion to the IgG N-terminus; 2) removal of the IgG light chain or Fab regions; 3) addition of hexamer-inducing mutations in the IgG Fc; 4) adding a self-binding epitope tag to create recombinant immune complexes (RIC); or 5) producing IgG fusions in plants that lack plant-specific β1,2-linked xylose and α1,3-linked fucose N-linked glycans.
35211008	11	97	gly	N-glycosylation	1915:1929	arg1	β1 adrenergic receptors	β1 adrenergic receptors				Fterm		receptors			The N-glycosylation of β1 adrenergic receptors decreased after continuous isoproterenol stimulation, while stachytine hydrochloride can increase the N-glycosylation of β1AR in the heart of mice with isoproterenol-induced heart failure.
36838558	1	31	gly	protein	255:261	arg1	the GalNAc-attached form	protein			the GalNAc-attached form	Fterm		protein			O-linked α-N-acetylgalactosamine (α-GalNAc) in the Gc protein is essential for macrophage activation; thus, the GalNAc-attached form of Gc protein is called Gc macrophage activating factor (GcMAF).
35193013	1	1	gly	glycoprotein	197:208	arg1	serum glycoprotein N-glycans				serum glycoprotein N-glycans						Characterization of serum glycoprotein N-glycans with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in positive-ion mode needs a derivatization step to stabilize and neutralize the negative charge on sialic acids.
36565991	3	25	gly	glycosylation	357:369	arg2	glycosylation attachment sites			glycosylation attachment sites						sites	One class of mutation impacts glycosylation attachment sites, which have the capacity to influence the antigenic structure beyond the immediate site of attachment.
35569685	1	38	gly	origin	302:307	arg1	three polysaccharide gums	origin			three polysaccharide gums	Fterm		origin			A facile, green synthesis methodology to obtain zinc oxide nanoparticles using three polysaccharide gums (Acacia gum, Guar gum and Xanthan gum) of biological origin was developed.
36409896	4	10	gly	glycoforms	630:639	arg1	IgG	IgG				Cterm		IgG			We apply this technology to immunoglobulin G (IgG) Fc glycoforms and define nanobodies that specifically recognize either IgG lacking its core-fucose or IgG bearing terminal sialic acid residues.
36409896	4	10	gly	glycoforms	630:639	arg1	immunoglobulin G	immunoglobulin G				Cterm		immunoglobulin G			We apply this technology to immunoglobulin G (IgG) Fc glycoforms and define nanobodies that specifically recognize either IgG lacking its core-fucose or IgG bearing terminal sialic acid residues.
33073321	6	61	gly	glycopeptides	1436:1448	arg2	the identified glycopeptides			the identified glycopeptides						glycopeptides	Further gene ontology analysis of molecular function and biological process revealed that 13 original glycoproteins of the identified glycopeptides from urine of patients significantly participate in diverse cancer-associated events, including collagen binding, immunoglobulin receptor binding, antigen binding, and complement activation process.
33073321	6	67	gly	glycoproteins	1404:1416	arg1	13 original glycoproteins	13 original glycoproteins				Fterm		glycoproteins			Further gene ontology analysis of molecular function and biological process revealed that 13 original glycoproteins of the identified glycopeptides from urine of patients significantly participate in diverse cancer-associated events, including collagen binding, immunoglobulin receptor binding, antigen binding, and complement activation process.
35802124	3	20	gly	region	685:690	arg1	the tissue				the tissue						Unstandardized and uncontrolled incubation steps often cause significant delocalization of released N-glycans, resulting in the inability to link given N-glycan composition to a specific microanatomical region in the tissue.
37287875	2	37	gly	glycopeptide	219:230	arg2	the intact glycopeptide level			the intact glycopeptide level						glycopeptide	Glycosylation has been increasingly analyzed at the intact glycopeptide level using mass spectrometry to study site-specific glycosylation changes under different physiological and pathological conditions.
36018613	11	64	gly	fragment	1269:1276	arg1	the m/z 204, C8H14N1O5+, glycan fragment population				the m/z 204, C8H14N1O5+, glycan fragment population						IR action spectra for the m/z 204, C8H14N1O5+, glycan fragment population support this prediction.
34985300	12	56	gly	glycosylation	2174:2186	arg2	257 glycosylation sites			257 glycosylation sites						sites	This study has for the first time demonstrated the presence of O-linked glycosylation in this organism by determining the basic structure of the O-glycans and identifying 257 glycosylation sites in 145 proteins.
36493594	1	1	gly	N-glycans	192:200	arg1	glycoproteins	glycoproteins			N-glycans	Fterm		glycoproteins			BACKGROUND N-glycans in glycoproteins can affect physicochemical properties of proteins; however, some reported N-glycan structures are inconsistent depending on the type of glycoprotein or the preparation methods.
36493594	1	11	gly	glycoproteins	205:217	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			BACKGROUND N-glycans in glycoproteins can affect physicochemical properties of proteins; however, some reported N-glycan structures are inconsistent depending on the type of glycoprotein or the preparation methods.
36493594	1	19	gly	glycoprotein	355:366	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			BACKGROUND N-glycans in glycoproteins can affect physicochemical properties of proteins; however, some reported N-glycan structures are inconsistent depending on the type of glycoprotein or the preparation methods.
35373202	4	64	gly	glycosylation	833:845	arg2	the glycosylation site selection			the glycosylation site selection						site	By using MUC1, with four tandem repeat domains as a substrate, we confirmed the glycosylation preferences of different GalNAc-Ts isoforms and highlighted the importance of the lectin domain in the glycosylation site selection after the addition of the first GalNAc residue.
35320529	4	44	gly	composition	666:676	arg1	primary lymphocyte membrane receptors	receptors			composition	Fterm		receptors			However, the site-specific glycan composition on primary lymphocyte membrane receptors in healthy individuals is largely limited.
35995381	7	82	gly	6 N-glycosylation	1078:1094	arg2	6 N-glycosylation sites			6 N-glycosylation sites						sites	It was noticed that 6 N-glycosylation sites contain only high-mannose type glycans, while other regions include complex and hybrid type glycans.
35995381	7	38	gly	contain	1102:1108	arg1	6 N-glycosylation sites AND high-mannose type glycans			6 N-glycosylation sites	high-mannose type glycans					sites	It was noticed that 6 N-glycosylation sites contain only high-mannose type glycans, while other regions include complex and hybrid type glycans.
37348475	6	17	gly	glycosylation	1191:1203	arg1	the AGP precursor	the AGP precursor				Fterm		precursor			These observations indicate that GPI-anchoring is required for the proper transport and glycosylation of the AGP precursor.
34864901	6	20	gly	proteins	1066:1073	arg1	the glycan shielding	proteins			the glycan shielding	Fterm		proteins			Overall, GLYCO provides fundamental insights into the glycan shielding of glycosylated proteins.
34864901	6	47	gly	glycosylated	1053:1064	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			Overall, GLYCO provides fundamental insights into the glycan shielding of glycosylated proteins.
35995381	15	61	gly	glycosylation	2267:2279	arg2	all determined glycosylation sites			all determined glycosylation sites						sites	In addition, N-glycan structures containing core-fucosylation and bisecting types were confirmed for all determined glycosylation sites.
35995381	11	45	gly	N-glycosylation	1648:1662	arg2	the N-glycosylation sites	thyroglobulin		sites		PUBTATOR		thyroglobulin	7038	sites	Therefore, a comprehensive analysis of the N-glycosylation sites of human thyroglobulin is essential to improve our understanding of the function of its N-glycans.
33904933	0	30	gly	antigen	48:54	arg1	annexin A2 protein	annexin A2 protein			antigen	PUBTATOR		annexin A2 protein	302		Human colorectal cancer-associated carbohydrate antigen on annexin A2 protein.
33316265	12	7	gly	eNOS	1681:1684	arg1	decreased O-GlcNAc modification	eNOS			decreased O-GlcNAc modification	PUBTATOR		eNOS	24600		SIGNIFICANCE Increased OGA activity and decreased O-GlcNAc modification of eNOS boosts eNOS activity in arteries of P-Wistar rats.
33316265	12	7	gly	eNOS	1681:1684	arg1	SIGNIFICANCE Increased OGA activity and decreased O-GlcNAc modification	eNOS			SIGNIFICANCE Increased OGA activity and decreased O-GlcNAc modification	PUBTATOR		eNOS	24600		SIGNIFICANCE Increased OGA activity and decreased O-GlcNAc modification of eNOS boosts eNOS activity in arteries of P-Wistar rats.
33316265	12	8	gly	modification	1665:1676	arg3	eNOS AND decreased O-GlcNAc modification	eNOS			decreased O-GlcNAc modification	PUBTATOR		eNOS	24600		SIGNIFICANCE Increased OGA activity and decreased O-GlcNAc modification of eNOS boosts eNOS activity in arteries of P-Wistar rats.
36014368	0	61	gly	N-Glycosylation	21:35	arg1	the SARS-CoV-2 S Protein	the SARS-CoV-2 S Protein				PUBTATOR		S Protein	7448		Investigation of the N-Glycosylation of the SARS-CoV-2 S Protein Contained in VLPs Produced in Nicotiana benthamiana.
37162352	3	0	gly	O-glycosylation	386:400	arg1	S-layer proteins	S-layer proteins				Fterm		proteins			O-Glycosylation has been reported in several Bacteroidota species, and the O-glycosylation of S-layer proteins in Tannerella forsythia was shown to be important for certain virulence features.
36370046	1	16	gly	protein	149:155	arg1	Glycans	spike protein			Glycans	PUBTATOR		spike protein	43740568		Glycans of the SARS-CoV-2 spike protein are speculated to play functional roles in the infection processes as they extensively cover the protein surface and are highly conserved across the variants.
35311852	3	100	gly	microheterogeneity	423:440	arg1	glycan isomeric structures				glycan isomeric structures						However, the separation and identification of glycan isomers has been a challenge for years due to the microheterogeneity of glycan isomeric structures.
37382290	7	31	gly	N-glycopeptide	1223:1236	arg2	synthetic stable isotope-labeled N-glycopeptide standards			synthetic stable isotope-labeled N-glycopeptide standards						N-glycopeptide	Here, we particularly focused on N-glycoproteomics analysis and investigated fragmentation specificity using synthetic stable isotope-labeled N-glycopeptide standards.
35421698	2	45	gly	N-glycopeptides	276:290	arg2	the intact N-glycopeptides			N-glycopeptides	Monosaccharide composition-level characterization					N-glycopeptides	Monosaccharide composition-level characterization at the intact N-glycopeptides has been extensively reported, yet structure-specific study to resolve multiple sequence structures of a single composition is still lacking.
35092134	7	8	gly	MET	1156:1158	arg1	N-glycan-deletion mutants			MET	N-glycan-deletion mutants					MET	To examine the effects of the deletion of N-glycans of MET, we prepared endogenous MET knockout Flp-In CHO cells and transfected them with a series of N-glycan-deletion mutants of MET.
35092134	7	38	gly	MET	1031:1033	arg1	N-glycans			MET	N-glycans					MET	To examine the effects of the deletion of N-glycans of MET, we prepared endogenous MET knockout Flp-In CHO cells and transfected them with a series of N-glycan-deletion mutants of MET.
37216524	10	60	gly	glycoproteins	1564:1576	arg1	GalNAcα1-O-Ser/Thr/Tyr	glycoproteins			GalNAcα1-O-Ser/Thr/Tyr	Fterm		glycoproteins			Although A20D-Cosmc is functional, its decreased expression, though in a cell or tissue-specific manner, causes a large reduction of T-synthase protein and activity, which accordingly leads to expression of varied amounts of pathological Tn-antigen (GalNAcα1-O-Ser/Thr/Tyr) on multiple glycoproteins.
36698045	10	58	gly	19 N-glycosites	1343:1357	arg2	19 N-glycosites			19 N-glycosites						19 N-glycosites	As a result, a total of 19 N-glycosites were identified in the recombinant pike protein at intact N-glycopeptide level.
36698045	10	84	gly	N-glycopeptide	1417:1430	arg2	intact N-glycopeptide level			intact N-glycopeptide level						N-glycopeptide	As a result, a total of 19 N-glycosites were identified in the recombinant pike protein at intact N-glycopeptide level.
35651042	5	4	gly	TFP	794:796	arg1	free monosaccharide	TFP			free monosaccharide	OGER		TFP	Q9HCM9		However, the physicochemical characteristics of TFP, including reducing sugar content, molecular weight, constituent monosaccharide, and free monosaccharide released, were obviously altered after the in vitro fermentation for 48 h, indicating that it was remarkably utilized by intestinal microbiota in human feces.
35651042	5	4	gly	TFP	794:796	arg1	reducing sugar content	TFP			reducing sugar content	OGER		TFP	Q9HCM9		However, the physicochemical characteristics of TFP, including reducing sugar content, molecular weight, constituent monosaccharide, and free monosaccharide released, were obviously altered after the in vitro fermentation for 48 h, indicating that it was remarkably utilized by intestinal microbiota in human feces.
35651042	5	4	gly	TFP	794:796	arg1	constituent monosaccharide	TFP			constituent monosaccharide	OGER		TFP	Q9HCM9		However, the physicochemical characteristics of TFP, including reducing sugar content, molecular weight, constituent monosaccharide, and free monosaccharide released, were obviously altered after the in vitro fermentation for 48 h, indicating that it was remarkably utilized by intestinal microbiota in human feces.
36709835	6	11	gly	afucosylated	1021:1032	arg1	afucosylated, high-mannose glycans				afucosylated, high-mannose glycans						While Humira® had the fewest unique glycans, 15, and 11.4 ± 0.8% of afucosylated, high-mannose glycans.
36758646	9	54	gly	α-1,6-fucosylation	1363:1380	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Therefore, correct α-1,6-fucosylation of glycoproteins is important for N. lugens during embryonic development and this study provides new insights into the role of N-glycosylation in embryogenesis in insects.
36758646	9	62	gly	glycoproteins	1385:1397	arg1	correct α-1,6-fucosylation	glycoproteins			correct α-1,6-fucosylation	Fterm		glycoproteins			Therefore, correct α-1,6-fucosylation of glycoproteins is important for N. lugens during embryonic development and this study provides new insights into the role of N-glycosylation in embryogenesis in insects.
34726173	0	83	gly	glycosylation	45:57	arg1	staphylococcal adhesive proteins	staphylococcal adhesive proteins				Fterm		proteins			Structural basis for SdgB- and SdgA-mediated glycosylation of staphylococcal adhesive proteins.
37354227	6	57	gly	N-glycosylation	1062:1076	arg2	the N-glycosylation sequons			the N-glycosylation sequons							Some of the N-glycosylation sequons in S show differences between SARS-CoV-2 variants in the distribution of glycan forms.
35166741	0	52	gly	glycosylated	37:48	arg1	glycosylated interferon-β-polypeptide			glycosylated interferon-β-polypeptide						interferon-β-polypeptide	Optimizing the Semisynthesis towards glycosylated interferon-β-polypeptide by utilizing bacterial protein expression and chemical modification.
35316032	5	30	gly	glycosylated	1332:1343	arg1	both Fc and Fab domains			both Fc and Fab domains						domains	The chemoenzymatic approach was highly specific for Fc glycan remodeling when both Fc and Fab domains were glycosylated, as exemplified by the selective Fc-glycan remodeling of cetuximab.
34691043	1	45	gly	glycoproteins	176:188	arg1	its surface exposed glycoproteins	its surface exposed glycoproteins				Fterm		glycoproteins			Influenza virus alters glycosylation patterns on its surface exposed glycoproteins to evade host adaptive immune responses.
36534501	1	67	gly	leucine-rich	117:128	arg1	Small leucine-rich proteoglycans			leucine	Small leucine-rich proteoglycans					leucine	Small leucine-rich proteoglycans (SLRPs) are major regulators of extracellular matrix assembly and cell signaling.
31941975	8	44	gly	derived	1464:1470	arg1	54 glycoproteins AND 4 dual-fucosylation types	54 glycoproteins			4 dual-fucosylation types	Fterm		glycoproteins			A total of 82N-glycopeptides, with 54 core-, 24 outer-, and 4 dual-fucosylation types derived from 54 glycoproteins, were commonly classified as the same type in both the DNN and SVM.
31941975	8	62	gly	glycoproteins	1480:1492	arg1	54 glycoproteins	54 glycoproteins				Fterm		glycoproteins			A total of 82N-glycopeptides, with 54 core-, 24 outer-, and 4 dual-fucosylation types derived from 54 glycoproteins, were commonly classified as the same type in both the DNN and SVM.
31941975	8	81	gly	82N-glycopeptides	1389:1405	arg1	4 dual-fucosylation types			82N-glycopeptides	4 dual-fucosylation types					82N-glycopeptides	A total of 82N-glycopeptides, with 54 core-, 24 outer-, and 4 dual-fucosylation types derived from 54 glycoproteins, were commonly classified as the same type in both the DNN and SVM.
35304921	15	50	gly	glycoproteins	2201:2213	arg1	"native" glycoproteins	"native" glycoproteins				Fterm		glycoproteins			Furthermore, the α5 β1 integrin preparation that was analyzed here is from a natural source, which is of importance as there is not a lot of literature in the field about the glycosylation of "native" glycoproteins.
35304921	15	93	gly	glycosylation	2175:2187	arg1	"native" glycoproteins	"native" glycoproteins				Fterm		glycoproteins			Furthermore, the α5 β1 integrin preparation that was analyzed here is from a natural source, which is of importance as there is not a lot of literature in the field about the glycosylation of "native" glycoproteins.
36345209	9	11	gly	glycoproteins	1046:1058	arg1	Specific glycoproteins	Specific glycoproteins				Fterm		glycoproteins			Specific glycoproteins were found to increase in the 3D cell, elucidating the pathways that are affected between the two models.
36809652	5	27	gly	apo	894:896	arg1	the O-glycan structures	apo(a			the O-glycan structures	OGER		apo(a	P08519		Using apo(a), isolated from human plasma, we demonstrated the potential of the O-glycan structures of apo(a) in Lp(a) to inhibit angiogenic properties such as proliferation, migration, and tube-formation in human umbilical vein endothelial cells (HUVECs) as well as neovascularization in chick chorioallantoic membrane.
36809652	5	14	gly	structures	880:889	arg1	a	Lp(a			structures	OGER		Lp(a	P08519		Using apo(a), isolated from human plasma, we demonstrated the potential of the O-glycan structures of apo(a) in Lp(a) to inhibit angiogenic properties such as proliferation, migration, and tube-formation in human umbilical vein endothelial cells (HUVECs) as well as neovascularization in chick chorioallantoic membrane.
33801653	7	48	gly	4E-BP1	1433:1438	arg1	the S5A/S6A O-GlcNAcylation-site mutant	4E-BP1			the S5A/S6A O-GlcNAcylation-site mutant	PUBTATOR		4E-BP1	1978		Further analyses revealed that the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) contributes to the downregulation of OGT induced by hyper-O-GlcNAcylation; the S5A/S6A O-GlcNAcylation-site mutant of 4E-BP1 cannot support this regulation, suggesting an important role of O-GlcNAcylation.
36435935	8	0	gly	N-glycosylation	1142:1156	arg2	closely spaced N-glycosylation sites			closely spaced N-glycosylation sites						sites	This alternate priming allows OST to efficiently process closely spaced N-glycosylation sites.
35960654	5	17	gly	N-glycopeptides	981:995	arg2	3514 quantifiable N-glycopeptides			3514 quantifiable N-glycopeptides						N-glycopeptides	With the integration of a one-tube sample processing workflow and high-pH fractionation, 3514 quantifiable N-glycopeptides were identified from 30 μg HeLa cell tryptic digests with reliable quantification performance.
36940546	1	13	gly	-Rhap-	230:235	arg1	a novel 28.6 kDa acidic polysaccharide	Rhap-(1			a novel 28.6 kDa acidic polysaccharide	OGER		Rhap-(1			In this study, a novel 28.6 kDa acidic polysaccharide (HTP-1), containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues as the backbone, analogous to pectin, was isolated from mature Hawk tea leaves.
36940546	1	26	gly	1 → and → 3,6	237:249	arg1	a novel 28.6 kDa acidic polysaccharide	3,6)-Galp			a novel 28.6 kDa acidic polysaccharide	OGER		3,6)-Galp	Q9UBC7		In this study, a novel 28.6 kDa acidic polysaccharide (HTP-1), containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues as the backbone, analogous to pectin, was isolated from mature Hawk tea leaves.
36940546	1	32	gly	-Galp-	251:256	arg1	a novel 28.6 kDa acidic polysaccharide	3,6)-Galp			a novel 28.6 kDa acidic polysaccharide	OGER		3,6)-Galp	Q9UBC7		In this study, a novel 28.6 kDa acidic polysaccharide (HTP-1), containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues as the backbone, analogous to pectin, was isolated from mature Hawk tea leaves.
34726173	7	8	gly	glycosylation	1743:1755	arg1	the SDR domain			the SDR domain						domain	Overall, these findings not only elucidate the putative role of the three domains of SdgB in recognizing donor and acceptor substrates, but also provide new mechanistic insights into glycosylation of the SDR domain, which can serve as a starting point for the development of antibacterial drugs against staphylococcal infections.
36385894	7	95	gly	glycopeptides	1154:1166	arg2	The glycopeptides			The glycopeptides						glycopeptides	The glycopeptides involved proteins in complement and coagulation cascades (p adj = 6.418 × 10-4), innate immunity (p adj = 6.094 × 10-3), acute inflammatory response (p adj = 6.404 × 10-5), defense response (p adj = 2.082 × 10-4), complement activation pathways (p adj = 1.895 × 10-2), and immunoglobulin-mediated immune response pathways (p adj = 4.818 × 10-2).
36018613	9	92	gly	peptide	873:879	arg1	charged glycan and peptide fragments				charged glycan and peptide fragments						We then interrogate the structure(s) of the resulting charged glycan and peptide fragments with infrared "action" spectroscopy.
35995381	4	32	gly	fucosylated	767:777	arg1	all fucosylated N-glycans				all fucosylated N-glycans						The sulfated complex and hybrid type N-glycan species were determined by the analysis of the human Tg samples with HPLC-HILIC-FLD-MS/MS. It was found that all fucosylated N-glycans carried fucose residue on their N-glycan core structure.
32709309	1	1	gly	glycoproteins	204:216	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity.
32709309	1	23	gly	released	190:197	arg1	glycoproteins AND glycans	glycoproteins			glycans	Fterm		glycoproteins			Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity.
32691273	13	21	gly	proteins	1890:1897	arg1	reduced O-GlcNAcylation	proteins			reduced O-GlcNAcylation	Fterm		proteins			Moreover, reduced O-GlcNAcylation of the total proteins by the shRNA-mediated silencing of the OGT gene, which is the only gene to modify proteins with the O-GlcNAc molecule, reversed the insulin resistance and endoplasmic reticulum stress phenotype, even with the externally stimulated O-GlcNAc modification conditions.
32691273	13	109	gly	proteins	1981:1988	arg1	the O-GlcNAc molecule	proteins			the O-GlcNAc molecule	Fterm		proteins			Moreover, reduced O-GlcNAcylation of the total proteins by the shRNA-mediated silencing of the OGT gene, which is the only gene to modify proteins with the O-GlcNAc molecule, reversed the insulin resistance and endoplasmic reticulum stress phenotype, even with the externally stimulated O-GlcNAc modification conditions.
37218360	14	6	gly	glycoforms	1845:1854	arg1	optimal antibody and CD16a glycoforms	optimal antibody and CD16a glycoforms				OGER		CD16a	P08637		Furthermore, optimal antibody and CD16a glycoforms are defined that provide the greatest ADCC activity.
35021101	2	50	gly	glycosylated	314:325	arg1	glycosylated HIV Env-immunogen NPs	glycosylated HIV Env-immunogen NPs				OGER		NPs	P0C0P6		We recently reported that mannose-binding lectin (MBL) triggers trafficking of glycosylated HIV Env-immunogen NPs to lymph node follicles.
35713525	7	31	gly	N-glycan	874:881	arg1	Asn-51	Csh1		Asn-51	Asn-51		SpecificSite	Csh1	852458	Asn-51	Although intracellular proteins usually harbor core-type N-glycans, the N-glycan on Asn-51 of Csh1 exhibited a unique mannan-like structure containing a long backbone of mannose.
35370997	9	96	gly	released	2168:2175	arg1	IgG AND N-glycans	IgG			N-glycans	Cterm		IgG			N-glycans were released from IgG by PNGase F digestion and analyzed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) after 2-aminobenzamide (2-AB) labeling.
36252443	1	26	gly	glycoproteins	114:126	arg1	Milk glycoproteins	Milk glycoproteins				Fterm		glycoproteins			Milk glycoproteins play various biological roles including antibacterial, antiviral activities, modulating immune responses in living organisms.
36999031	6	5	gly	fucosylated	1463:1473	arg1	all fucosylated digalactosylated structures				all fucosylated digalactosylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36999031	6	11	gly	disialylated	1219:1230	arg1	all fucosylated monosyalilated and disialylated structures				all fucosylated monosyalilated and disialylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36999031	6	98	gly	fucosylated	1188:1198	arg1	all fucosylated monosyalilated and disialylated structures				all fucosylated monosyalilated and disialylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36999031	6	101	gly	fucosylated	1361:1371	arg1	all fucosylated structures				all fucosylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36740532	1	65	gly	glycoprotein	98:109	arg1	BACKGROUND Platelet glycoprotein	BACKGROUND Platelet glycoprotein				Fterm		glycoprotein			BACKGROUND Platelet glycoprotein (GP) Ibα is the major ligand-binding subunit of the GPIb-IX-V complex that binds von Willebrand factor.
36089065	1	17	gly	glycoprotein	208:219	arg1	various glycoprotein properties	various glycoprotein properties				Fterm		glycoprotein			N-glycosylation is an essential eukaryotic posttranslational modification that affects various glycoprotein properties, including folding, solubility, protein-protein interactions, and half-life.
36192063	5	37	gly	N-glycopeptides	873:887	arg2	TMT-labeled intact N-glycopeptides			TMT-labeled intact N-glycopeptides						N-glycopeptides	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
36192063	5	44	gly	N-glycosite	1216:1226	arg2	N-glycosite			N-glycosite						N-glycosite	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
36192063	5	84	gly	N-glycopeptide	1176:1189	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
36252443	8	27	gly	glycoproteins	1374:1386	arg1	dairy glycoproteins	dairy glycoproteins				Fterm		glycoproteins			In this study, the activity of EndoBI-1 was compared to a commercial enzyme to release N-glycans, the peptide-N-glycosidase F (PNGase F), using dairy glycoproteins as the substrate.
37156312	0	65	gly	glycosites	49:58	arg2	human O-linked threonine glycosites			human O-linked threonine glycosites						glycosites	O-GlyThr: Prediction of human O-linked threonine glycosites using multi-feature fusion.
37266972	0	54	gly	glycoproteins	39:51	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.
37266972	0	95	gly	deglycosylation	8:22	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.
35262690	10	75	gly	hyposialylation	1504:1518	arg1	O-glycans				O-glycans						It also points towards a possible mechanism behind the hyposialylation of N and O-glycans.
36606688	6	47	gly	SOD3	845:848	arg1	the N-glycan	SOD3			the N-glycan	PUBTATOR		SOD3	6649		In cell lines of non-small lung cancer cell (NSCLC), we also found a high level of the core fucose structure in the N-glycan of SOD3, as determined by lectin blotting and mass spectrometry analysis.
36660462	7	80	gly	glycoproteins	1243:1255	arg1	10 glycoproteins	10 glycoproteins				Fterm		glycoproteins			Through a combined analysis of proteomic and glycoproteomic data, it was found that the changes in 10 glycoproteins were caused by the difference in glycosylation level but not in the protein abundance level.
37037133	1	35	gly	glycosylated	210:221	arg1	Milk fat globule membrane (MFGM) proteins	Milk fat globule membrane (MFGM) proteins				Fterm		proteins			Milk fat globule membrane (MFGM) proteins are highly glycosylated and involved in various biological processes within the body.
37037133	2	58	gly	glycoproteins	347:359	arg1	MFGM glycoproteins	MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		However, information on site-specific N-glycosylation of MFGM glycoproteins in donkey and human milk remains limited.
37037133	2	78	gly	N-glycosylation	323:337	arg1	MFGM glycoproteins	MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		However, information on site-specific N-glycosylation of MFGM glycoproteins in donkey and human milk remains limited.
36289103	8	24	gly	sialylated	1099:1108	arg1	α2-6 linked sialylated N-glycans				α2-6 linked sialylated N-glycans						Importantly, α2-6 linked sialylated N-glycans were dominant across most HNSCC cell lines except in SCC-9 cells where similar levels of α2-6 and α2-3 sialylated N-glycans were observed.
36289103	8	59	gly	sialylated	1223:1232	arg1	α2-6 and α2-3 sialylated N-glycans				α2-6 and α2-3 sialylated N-glycans						Importantly, α2-6 linked sialylated N-glycans were dominant across most HNSCC cell lines except in SCC-9 cells where similar levels of α2-6 and α2-3 sialylated N-glycans were observed.
37366623	2	112	gly	glycoprotein	395:406	arg1	envelope glycoprotein B	envelope glycoprotein B				Fterm		glycoprotein B			Here we show in cell cultures that an N-glycan shield on the herpes simplex virus 1 (HSV-1) envelope glycoprotein B (gB) mediated evasion from neutralization and antibody-dependent cellular cytotoxicity due to pooled γ-globulins derived from human blood.
35889277	2	14	gly	glycoproteins	377:389	arg1	glycoproteins	glycoproteins				Fterm		structure of glycoproteins			Hence, the development of analytical methods able to characterize the composition and structure of glycoproteins is crucial.
37211816	5	54	gly	N-glycosylation	1224:1238	arg2	optimal artificial VHH N-glycosylation sites			optimal artificial VHH N-glycosylation sites						sites	The set of optimal artificial VHH N-glycosylation sites identified in this study can serve as a blueprint for targeted glyco-engineering of other VHHs, enabling site-specific functionalization through the rapidly expanding toolbox of synthetic glycobiology.
35766466	8	26	gly	nonsialylated	1273:1285	arg1	nonsialylated N-glycans				nonsialylated N-glycans						Improving detection of nonsialylated N-glycans allows for a more thorough analysis of specific structural features such as fucosylation or branching, particularly of low abundant structures.
36189205	6	17	gly	afucosylated	1115:1126	arg1	afucosylated IgG1	afucosylated IgG1				OGER		IgG1	P01857		Our study confirms that afucosylated IgG1 has the highest binding affinity to oligomannose FcγRIIIa, a glycan structure commonly found on Asn162 on FcγRIIIa expressed by NK cells but not monocytes or recombinantly expressed FcγRIIIa.
36189205	6	47	gly	found	1220:1224	arg1	Asn162 AND a glycan structure	FcγRIIIa		Asn162	a glycan structure	PUBTATOR		FcγRIIIa	2214	Asn162	Our study confirms that afucosylated IgG1 has the highest binding affinity to oligomannose FcγRIIIa, a glycan structure commonly found on Asn162 on FcγRIIIa expressed by NK cells but not monocytes or recombinantly expressed FcγRIIIa.
33515675	0	50	gly	NOTCH1	57:62	arg1	O-GlcNAcylation	NOTCH1			O-GlcNAcylation	PUBTATOR		NOTCH1	4851		SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.
35413075	10	36	gly	α1,3-fucosylation	1468:1484	arg1	glycans				glycans						Among them, glycans with α1,3-fucosylation ((+/- sialyl) Lewis X), triply α2,6-sialylated tri-antennary structures and/or a (Man3)GlcNAc1-core displayed elevated levels in cancer patients.
35413075	10	86	gly	α2,6-sialylated	1517:1531	arg1	triply α2,6-sialylated tri-antennary structures				triply α2,6-sialylated tri-antennary structures						Among them, glycans with α1,3-fucosylation ((+/- sialyl) Lewis X), triply α2,6-sialylated tri-antennary structures and/or a (Man3)GlcNAc1-core displayed elevated levels in cancer patients.
36585837	5	28	gly	glycosylation	785:797	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Glycopeptides were isolated with lectin-based extraction and treated with Peptide N-glycosidase F (PNGase F) to identify N-linked glycosylation sites.
35380435	9	26	gly	glycoproteins	1836:1848	arg1	heterogeneous glycoproteins	heterogeneous glycoproteins				Fterm		glycoproteins			The HILIC top-down MS platform holds great potential in resolving heterogeneous glycoproteins for facile comparison of biosimilars in quality control applications.
37266972	10	17	gly	glycoprotein	1258:1269	arg1	glycoprotein B	glycoprotein B				Cterm		glycoprotein B			Likewise, extensive deglycosylation of glycoprotein B, which possesses 18 N-glycosylation sites, was observed.
37266972	10	20	gly	deglycosylation	1239:1253	arg1	glycoprotein B	glycoprotein B				Cterm		glycoprotein B			Likewise, extensive deglycosylation of glycoprotein B, which possesses 18 N-glycosylation sites, was observed.
37266972	10	21	gly	18 N-glycosylation	1290:1307	arg2	18 N-glycosylation sites			18 N-glycosylation sites						sites	Likewise, extensive deglycosylation of glycoprotein B, which possesses 18 N-glycosylation sites, was observed.
37289618	6	15	gly	sialylated	1121:1130	arg1	sialylated N-linked glycans				sialylated N-linked glycans						This new rule grants confident glycan annotations and compositions beyond accurate mass measurements, thereby further improving the capability of IR-MALDESI to study sialylated N-linked glycans within biological tissues.
35775947	5	45	gly	glycosylated	1192:1203	arg1	the galactose residue				the galactose residue						Starting with a common lactose precursor, the sulfur function was incorporated by double inversion of the configuration of the galactose residue that was further glycosylated using different activated donors.
32719124	3	15	gly	glycosylation	386:398	arg2	an N-linked glycosylation site			an N-linked glycosylation site						site	By engineering a glycoprotein-derived epitope to contain an N-linked glycosylation site, we determined that optimal CD8+ T cell expansion and function were induced by the peptides that are rapidly produced from the exceedingly minor fraction of protein mislocalized to the cytosol.
36830744	7	20	gly	composition	1251:1261	arg1	CAD			position	CAD					position	The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD.
35470665	8	46	gly	epitopes	1432:1439	arg1	gp120	gp120			epitopes	PUBTATOR		gp120	3700		These results demonstrated that the immune tolerance mechanism suppressed the immune responses to Man5-related structures and the conformation of glycan epitopes on the synthesized glycoconjugates was distinct from that of native glycan epitopes on gp120.
37341462	5	14	gly	O-glycopeptide	946:959	arg2	O-glycopeptide spectra			O-glycopeptide spectra						O-glycopeptide	In this strategy, theoretical O-glycan Y-ion patterns are constructed to match the experimental Y-ions in O-glycopeptide spectra, which enables the determination of the mass of some glycans and results in the reduction of searching space.
37121976	6	51	gly	deglycosylated	1200:1213	arg1	deglycosylated LRG1	deglycosylated LRG1				OGER		LRG1	P02750		Moreover, the intracavernous administration of deglycosylated LRG1 in a diabetic mouse model ameliorated vascular and neurological abnormalities and restored erectile function.
34674311	7	88	gly	glycosylation	1194:1206	arg1	TMEM43S358L	TMEM43S358L				PUBTATOR		TMEM43	79188		Intriguingly, the specific glycosylation of TMEM43S358L resulted from the altered membrane topology of TMEM43.
36935145	3	23	gly	glycoproteins	1024:1036	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Both LoG and GoG generates new structures and functions of glycoproteins, which has been observed in the S protein of SARS-Cov-2 as well as malignant diseases.
37348475	5	16	gly	proteins	1079:1086	arg1	the glycan structures	proteins			the glycan structures	Fterm		proteins			Comparison of the glycan structures of the microsomal SPO-AGP and the secreted SPO-AGPΔC using antibodies against the glycan epitopes of AGP indicated that the glycan structures of these proteins are different.
37348475	5	22	gly	SPO-AGP	946:952	arg1	the glycan structures	SPO			the glycan structures	OGER		SPO	P22079		Comparison of the glycan structures of the microsomal SPO-AGP and the secreted SPO-AGPΔC using antibodies against the glycan epitopes of AGP indicated that the glycan structures of these proteins are different.
37348475	5	54	gly	AGP	1029:1031	arg1	the glycan epitopes	AGP			the glycan epitopes	Cterm		AGP			Comparison of the glycan structures of the microsomal SPO-AGP and the secreted SPO-AGPΔC using antibodies against the glycan epitopes of AGP indicated that the glycan structures of these proteins are different.
35104505	7	6	gly	glycoprotein	1329:1340	arg1	the glycoprotein substrates	the glycoprotein substrates				Fterm		glycoprotein			Surprisingly, GnT-VΔN showed substantially reduced activity toward the glycoprotein substrates, whereas it almost fully maintained its activity toward the oligosaccharides and the glycopeptide substrates.
35104505	7	21	gly	glycopeptide	1438:1449	arg2	the glycopeptide substrates			the glycopeptide substrates						glycopeptide	Surprisingly, GnT-VΔN showed substantially reduced activity toward the glycoprotein substrates, whereas it almost fully maintained its activity toward the oligosaccharides and the glycopeptide substrates.
32273875	2	42	gly	carbohydrates	251:263	arg1	factor VIII	factor VIII			carbohydrates	PUBTATOR		factor VIII	14069		Oligomannose carbohydrates at N239 and/or N2118 on factor VIII allow its binding to the macrophage mannose receptor expressed on human dendritic cells, thereby leading to factor VIII endocytosis and presentation to CD4+ T lymphocytes.
32273875	2	46	gly	N239	268:271	arg1	Oligomannose carbohydrates	factor VIII		N239	Oligomannose carbohydrates			factor VIII	14069	N239	Oligomannose carbohydrates at N239 and/or N2118 on factor VIII allow its binding to the macrophage mannose receptor expressed on human dendritic cells, thereby leading to factor VIII endocytosis and presentation to CD4+ T lymphocytes.
32273875	2	51	gly	N2118	280:284	arg1	Oligomannose carbohydrates	factor VIII		N2118	Oligomannose carbohydrates			factor VIII	14069	N2118	Oligomannose carbohydrates at N239 and/or N2118 on factor VIII allow its binding to the macrophage mannose receptor expressed on human dendritic cells, thereby leading to factor VIII endocytosis and presentation to CD4+ T lymphocytes.
35887202	2	8	gly	glycosylation	280:292	arg1	MUC1				MUC1						This is based on the fact that MUC1 undergoes aberrant glycosylation upon cancer progression, and anti-MUC1 antibodies differentiate changes in glycan structure.
36637420	3	2	gly	HAI-2	506:510	arg1	The N-glycan moiety	HAI-2			The N-glycan moiety	PUBTATOR		HAI-2	10653		The N-glycan moiety of HAI-2 can function as a subcellular targeting signal.
35388686	1	35	gly	proteins	250:257	arg1	monosaccharide linkage patterns	proteins			monosaccharide linkage patterns	Fterm		proteins			Characterization of protein glycosylation by tandem mass spectrometry remains challenging owing to the vast diversity of oligosaccharides bound to proteins, the variation in monosaccharide linkage patterns, and the lability of the linkage between the glycan and protein.
35285541	2	9	gly	glycoproteins	274:286	arg1	wherein	wherein				Fterm		wherein			Majority of the biopharmaceuticals are glycoproteins, wherein about 1 to 30% of their molecular weight (MW) are contributed by the glycans.
35285541	2	9	gly	glycoproteins	274:286	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Majority of the biopharmaceuticals are glycoproteins, wherein about 1 to 30% of their molecular weight (MW) are contributed by the glycans.
35766466	0	33	gly	Nonsialylated	45:57	arg1	Nonsialylated N-Linked Glycans				Nonsialylated N-Linked Glycans						Novel Combined Enzymatic Approach to Analyze Nonsialylated N-Linked Glycans through MALDI Imaging Mass Spectrometry.
34452239	4	85	gly	glycopeptide	981:992	arg2	the glycopeptide			the glycopeptide						glycopeptide	We have synthesized the native Ang (1-7) peptide and the glycopeptide, PNA5, and have formulated them for targeted respiratory delivery as inhalable dry powders.
36528711	2	46	gly	deglycosylation	350:364	arg1	human IgG	human IgG				Cterm		IgG			The Streptococcus pyogenes bacterium secretes the protease IdeS and the glycosidase EndoS, which specifically catalyse cleavage and deglycosylation of human IgG, respectively.
37354227	0	75	gly	N-glycosylation	16:30	arg1	the spike proteins	the spike proteins				Fterm		proteins			Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2.
33199824	10	45	gly	O-GlcNAcylation	1191:1205	arg1	SMAD4 Thr63	SMAD4			O-GlcNAcylation	PUBTATOR		SMAD4	4089		As a result, defects in O-GlcNAcylation on SMAD4 Thr63 attenuated the reporter activity of luciferase, the TGF-β-responsive SMAD binding element (SBE).
36512245	1	4	gly	glycosylation	158:170	arg2	the major sites			the major sites						sites	The Golgi apparatus is one of the major sites of protein and lipid glycosylation and processing.
34379775	7	75	gly	fucosylated	1380:1390	arg1	fucosylated glycan				fucosylated glycan						There was a higher abundance of the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc), fucosylated glycan (GlcNAcβ1-3(Fucα1-2Galβ1-3)GalNAc) and core 4 glycan (GlcNAcβ1-3[GlcNAcβ1-6]GalNAc) in the low-fertility Suffolk breed compared with NWS (high fertility).
34379775	7	75	gly	fucosylated	1380:1390	arg1	the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc)				the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc)						There was a higher abundance of the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc), fucosylated glycan (GlcNAcβ1-3(Fucα1-2Galβ1-3)GalNAc) and core 4 glycan (GlcNAcβ1-3[GlcNAcβ1-6]GalNAc) in the low-fertility Suffolk breed compared with NWS (high fertility).
36797772	1	26	gly	glycoproteins	101:113	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The role of glycoproteins as key cell surface molecules during development and stress is well established, yet the relationship between their structural features and functional mechanisms are poorly defined.
35373202	6	16	gly	glycosylation	1158:1170	arg1	acceptor sites			acceptor sites						sites	Our experiments suggest that during this process, neighboring tandem repeats are critical for further glycosylation of acceptor sites by GalNAc-T2/T4 in a lectin-assisted manner.
34633871	9	28	gly	glycoproteins	1296:1308	arg1	volcanii glycoproteins	volcanii glycoproteins				Fterm		glycoproteins			volcanii glycoproteins.
34831340	4	4	gly	sialylated	631:640	arg1	sialylated N-linked glycans				sialylated N-linked glycans						We conducted a HPLC analysis of sialylated N-linked glycans released from total plasma proteins and characterized the α1,2-mannosidase I activity of the lymphocyte microsome fraction.
34831340	4	12	gly	released	659:666	arg1	total plasma proteins AND sialylated N-linked glycans	total plasma proteins			sialylated N-linked glycans	Fterm		proteins			We conducted a HPLC analysis of sialylated N-linked glycans released from total plasma proteins and characterized the α1,2-mannosidase I activity of the lymphocyte microsome fraction.
34611869	3	79	gly	glycans	653:659	arg1	the CH2 domains			the CH2 domains	the CH2 domains		Site			domains	In this report we analyzed four antibodies available in the PDB (1IGT, 1IGY, 1HZH, and 5DK3) using the online software UCSF Chimera to study the structural features of the proteins and the associated N-linked glycans in the CH2 domains of the Fc region.
34611869	3	79	gly	glycans	653:659	arg1	the Fc region			the Fc region	the Fc region		Site			region	In this report we analyzed four antibodies available in the PDB (1IGT, 1IGY, 1HZH, and 5DK3) using the online software UCSF Chimera to study the structural features of the proteins and the associated N-linked glycans in the CH2 domains of the Fc region.
36181511	5	49	gly	glycopeptide	1044:1055	arg2	an optional glycopeptide enrichment approach			an optional glycopeptide enrichment approach						glycopeptide	In conclusion, cotton HILIC can be used as an optional glycopeptide enrichment approach in glycosylation analysis with its specific merit.
36377874	1	72	gly	protein	150:156	arg1	The glycan loop	envelope protein			The glycan loop	PUBTATOR		envelope protein	64006		The glycan loop of Zika virus (ZIKV) envelope protein (E) contains the glycosylation site and has been well documented to be important for viral pathogenesis and transmission.
36377874	1	100	gly	glycosylation	175:187	arg2	the glycosylation site			the glycosylation site						site	The glycan loop of Zika virus (ZIKV) envelope protein (E) contains the glycosylation site and has been well documented to be important for viral pathogenesis and transmission.
35038468	1	22	gly	used	312:315	arg2	GLU-CPS-1			GLU-CPS-1						GLU	Two capsular polysaccharides (WL-CPS-1 and GLU-CPS-1) purified from Nostoc flagelliforme under normal and mixotrophic culture conditions were used to investigate the hypolipidemic activity and effect on intestinal flora in C57BL/6J mice respectively.
36007953	5	8	gly	core-fucosylated	957:972	arg1	the core-fucosylated N-glycans				the core-fucosylated N-glycans						The affinities were at a low nanomolar level, which were ~ 1000-fold stronger than those between PhoSL and the core-fucosylated N-glycans at the micromolar level.
36977665	2	7	gly	sequence	318:325	arg1	sequence glycan				sequence glycan						Nanopore-based single-molecule sensing offers the potential to elucidate glycan structure and even sequence glycan.
32417172	9	56	gly	glycosylated	1318:1329	arg1	the glycosylated sites			the glycosylated sites						sites	Ser121 residue was one of the glycosylated sites on p53.
37173020	2	46	gly	heterogeneity	491:503	arg1	pectin	pectin				Fterm		pectin			Numerous bioactive polysaccharides associated with pectin are newly reported every year, but the general mechanism of their immunological action remains unclear owing to the complexity and heterogeneity of pectin.
33244686	6	29	gly	N-glycosylation	1695:1709	arg2	five N-glycosylation sites			five N-glycosylation sites						sites	We demonstrated that with enhanced detection sensitivity, IP-HILIC-MS can confidently identify an increased number of site-specific N-linked glycans for IgG1, and IgG4 mAbs as well as an Fc-domain fusion protein (containing five N-glycosylation sites) through MS/MS-based search in the data-dependent acquisition mode, meanwhile, achieve comparable quantitative results compared with the traditional methods.
36007953	4	44	gly	core-fucosylated	818:833	arg1	core-fucosylated N-glycans				core-fucosylated N-glycans						Here, we observed that the S proteins of the ancestral (Wuhan) and Omicron strains bind with Pholiota squarrosa lectin (PhoSL), a 40-amino-acid chemically synthesised peptide specific to core-fucosylated N-glycans.
35616904	8	12	gly	glycoproteins	1380:1392	arg1	their shared glycoproteins	their shared glycoproteins				Fterm		glycoproteins			Comparison of site-specific glycans between seminal plasma and spermatozoa revealed more complicated glycan structures in seminal plasma than in spermatozoa, even on their shared glycoproteins.
37121976	5	1	gly	deglycosylation	884:898	arg1	LRG1	LRG1				OGER		LRG1	P02750		In addition, our biochemical and cell-biological analyses found that the deglycosylation of LRG1, particularly the removal of glycans on N325, is critical for the high-affinity binding of LRG1 to LPHN2 and thus promotes LRG1/LPHN2-mediated angiogenic and neurotrophic processes in mouse tissue explants, even under normal glucose conditions.
35977913	2	8	gly	N-glycosylated	143:156	arg1	N-glycosylated proteins	N-glycosylated proteins				Fterm		proteins			However, N-glycosylated proteins from natural sources exhibit considerable heterogeneity in the appended oligosaccharides, bringing daunting challenges to corresponding basic research and therapeutic applications.
35960654	1	18	gly	glycopeptide	134:145	arg2	Intact glycopeptide analysis			Intact glycopeptide analysis						glycopeptide	Intact glycopeptide analysis has been of great interest because it can elucidate glycosylation site information and glycan structural composition at the same time.
35960654	1	61	gly	glycosylation	208:220	arg2	glycosylation site information			glycosylation site information						site	Intact glycopeptide analysis has been of great interest because it can elucidate glycosylation site information and glycan structural composition at the same time.
36732731	7	122	gly	fucosylated	1290:1300	arg1	multiple fucosylated glycans				multiple fucosylated glycans						Lectin like activity at the N-terminal of TpsA due to a conserved hemagglutinin domain (Pfam identifier [ID] PF05860) mediates binding to mucins that carry multiple fucosylated glycans.
33446867	1	5	gly	glycosylation	190:202	arg1	proteins	proteins				Fterm		proteins			The oligosaccharide required for asparagine (N)-linked glycosylation of proteins in the endoplasmic reticulum (ER) is donated by the glycolipid Glc3Man9GlcNAc2-PP-dolichol.
36182101	8	59	gly	fucosylated	1552:1562	arg1	fucosylated structures				fucosylated structures						Statistical correlation analysis, binding experiments with J99 wt, and J99ΔbabAΔsabA and inhibition experiments using synthetic glycoconjugates demonstrated that the differences in H. pylori-binding ability among these four groups were governed by BabA-dependent binding to fucosylated structures.
31996426	6	67	gly	glycoprotein	922:933	arg1	the viral glycoprotein M envelope protein	the viral glycoprotein M envelope protein				Fterm		glycoprotein M			Despite the marked antimicrobial action of eBD2 and -3 against many bacterial and viral pathogens, EHV1 virions were resistant to eBDs through the action of the viral glycoprotein M envelope protein.
35921896	12	64	gly	heterogeneity	1568:1580	arg1	CD16a F158 backbone amide and N162 glycan resonances				CD16a F158 backbone amide and N162 glycan resonances						We also identified conformational heterogeneity of CD16a F158 backbone amide and N162 glycan resonances using NMR spectroscopy.
35179194	8	103	gly	-fucosylated	1328:1339	arg1	α(1,2)-fucosylated structures				α(1,2)-fucosylated structures						Genomic analyses of the mothers were not performed; instead, milk was phenotyped according to the abundances of α(1,2)-fucosylated structures.
37173020	5	25	gly	TLR4	956:959	arg1	leucine-rich repeats	TLR4			leucine-rich repeats	OGER		TLR4	O00206		Via structural investigation, the inner concavity of leucine-rich repeats of TLR4 was predicted to act as a binding motif for carbohydrate recognition, and subsequent simulations predicted the binding modes and conformations.
36791651	2	37	gly	CFF	458:460	arg1	polysaccharides	CFF			polysaccharides	OGER		CFF	P51610		Phytochemical studies found that phenylpropanoids, flavonoids, terpenoids and polysaccharides were the main ingredients of CFF.
36029899	6	25	gly	N-glycosylation	839:853	arg1	ADA2	ADA2				PUBTATOR		ADA2	51816		RESULTS Inhibiting the initial N-glycosylation of ADA2 in the ER via site-directed mutagenesis or treatment with N-glycosylation inhibitors reduced the intracellular ADA2 activity and secretion.
36493594	5	28	gly	RESULTS	977:983	arg1	IgG	IgG			RESULTS	Cterm		IgG			RESULTS The 21 N-glycans in fetuin and another 21 N-glycans in IgG by either PF-ProA or PA-ProA were identified using LC-MS/MS.
36698045	4	30	gly	N-glycosylated	559:572	arg1	the spike	the spike				PUBTATOR		spike	43740568		As a surface protein on the virus envelop, the spike was reported to be heavily N-glycosylated and glycosylation had a great impact on its immunogenicity and efficacy.
36698045	4	30	gly	N-glycosylated	559:572	arg1	a surface protein	a surface protein				Fterm		protein			As a surface protein on the virus envelop, the spike was reported to be heavily N-glycosylated and glycosylation had a great impact on its immunogenicity and efficacy.
36740532	10	76	gly	macroglycopeptide	1366:1382	arg2	macroglycopeptide domain			macroglycopeptide domain						macroglycopeptide domain	Glycosites are also located within the macroglycopeptide domain and mechanosensory domain.
35995381	12	69	gly	N-glycosylation	1837:1851	arg1	human thyroid thyroglobulin protein	human thyroid thyroglobulin protein				OGER		thyroglobulin protein	P01266		The present research significantly expanded the knowledge regarding N-glycosylation profiles of human thyroid thyroglobulin protein.
35889277	5	7	gly	glycoproteins	839:851	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
35889277	5	23	gly	composition	957:967	arg1	monosaccharides			position	monosaccharides					position	A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
35889277	5	23	gly	composition	957:967	arg1	glycans			position	glycans					position	A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
35889277	5	28	gly	glycoproteins	872:884	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
37266972	1	84	gly	glycoproteins	117:129	arg1	recombinant pharmaceutical glycoproteins	recombinant pharmaceutical glycoproteins				Fterm		glycoproteins			Production of recombinant pharmaceutical glycoproteins has been carried out in multiple expression systems.
36447399	5	30	gly	N-glycosylation	1193:1207	arg2	N-glycosylation sites			N-glycosylation sites						sites	Since only about 20% of human IgG1 have a Fab glycan, we extended the application of this approach by using molecular modeling to introduce N-glycosylation sites in the Fab constant region of other therapeutic monoclonal antibodies.
36447399	5	71	gly	have	1088:1091	arg1	human IgG1 AND a Fab glycan	human IgG1			a Fab glycan	OGER		IgG1	P01857		Since only about 20% of human IgG1 have a Fab glycan, we extended the application of this approach by using molecular modeling to introduce N-glycosylation sites in the Fab constant region of other therapeutic monoclonal antibodies.
36373229	8	9	gly	O-glycosylated	1826:1839	arg1	densely O-glycosylated mucin-domain glycoproteins	densely O-glycosylated mucin-domain glycoproteins				Fterm		glycoproteins			We use this platform to generate a consensus motif for the recently characterized immunomodulating metalloprotease (IMPa) from Pseudomonas aeruginosa and show that IMPa is a favorable O-glycoprotease for characterizing densely O-glycosylated mucin-domain glycoproteins.
36373229	8	11	gly	glycoproteins	1854:1866	arg1	densely O-glycosylated mucin-domain glycoproteins	densely O-glycosylated mucin-domain glycoproteins				Fterm		glycoproteins			We use this platform to generate a consensus motif for the recently characterized immunomodulating metalloprotease (IMPa) from Pseudomonas aeruginosa and show that IMPa is a favorable O-glycoprotease for characterizing densely O-glycosylated mucin-domain glycoproteins.
35647713	3	65	gly	glycoprotein	560:571	arg1	viral glycoprotein immunogens	viral glycoprotein immunogens				Fterm		glycoprotein			There is, however, a lack of methods to conjugate small molecule immune potentiators to viral glycoprotein immunogens without compromising epitope integrity.
37060669	2	38	gly	fucosylated	240:250	arg1	a fucosylated trisaccharide				a fucosylated trisaccharide						In this study, a fucosylated trisaccharide (GFG) was separated from the acidolysis products of exopolysaccharides from Clavibacter michiganensis M1.
37184080	3	14	gly	position	967:974	arg1	4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification			position	4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification					position	Using FLATn-Li, we elucidated the lipid A structure from several bacterial species, including novel structures from arctic bacterioplankton of the Duganella and Massilia genera that favor 4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification at the 1-phosphate position and that demonstrate double glycosylation with Ara4N at the 1 and 4' phosphate positions simultaneously.
37184080	3	60	gly	glycosylation	1004:1016	arg2	the 1 and 4' phosphate positions			positions						positions	Using FLATn-Li, we elucidated the lipid A structure from several bacterial species, including novel structures from arctic bacterioplankton of the Duganella and Massilia genera that favor 4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification at the 1-phosphate position and that demonstrate double glycosylation with Ara4N at the 1 and 4' phosphate positions simultaneously.
36329887	12	34	gly	glycoforms	2283:2292	arg1	different FSH glycoforms	different FSH glycoforms				OGER		FSH			Accordingly, the differences in binding capacity of the same receptor preparation to different FSH glycoforms are likely the organization of the FSH receptor in cell membranes, rather than the αAsn<sup>52</sup> oligosaccharide.
34939082	3	28	gly	sites	475:479	arg1	glycan modification			sites	glycan modification					sites	Here, we describe a simple, concise graphical representation intended to capture the microheterogeneity associated with glycan modification at specific sites.
35447118	1	32	gly	glycosylation	150:162	arg1	proteins	proteins				Fterm		proteins			Asparagine-linked glycosylation (N-glycosylation) of proteins in the cancer secretome has been gaining increasing attention as a potential biomarker for cancer detection and diagnosis.
34735575	4	49	gly	glycosylation	1053:1065	arg1	similarly produced spike proteins	similarly produced spike proteins				PUBTATOR		spike proteins	43740568		Despite maintaining an overall similar structural conformation, our mass spectrometry-based site-specific glycosylation analyses of similarly produced spike proteins with and without the D614G and Alpha variant mutations reveal a significant shift in the processing state of N-glycans on one specific NTD site.
37366623	0	38	gly	glycoprotein	48:59	arg1	the envelope glycoprotein B	the envelope glycoprotein B				Cterm		the envelope glycoprotein B			Dual impacts of a glycan shield on the envelope glycoprotein B of HSV-1: evasion from human antibodies in vivo and neurovirulence.
36985724	2	32	gly	heterogeneity	250:262	arg1	naturally occurring glycoproteins	naturally occurring glycoproteins				Fterm		glycoproteins			Due to N-glycan heterogeneity of naturally occurring glycoproteins, the functions of specific N-glycans on a particular glycoprotein are not always clear.
36985724	2	49	gly	glycoprotein	354:365	arg1	a particular glycoprotein	a particular glycoprotein				Fterm		glycoprotein			Due to N-glycan heterogeneity of naturally occurring glycoproteins, the functions of specific N-glycans on a particular glycoprotein are not always clear.
36985724	2	19	gly	glycoproteins	287:299	arg1	naturally occurring glycoproteins	glycoproteins			N-glycan heterogeneity	Fterm		glycoproteins			Due to N-glycan heterogeneity of naturally occurring glycoproteins, the functions of specific N-glycans on a particular glycoprotein are not always clear.
37294165	4	67	gly	N-glycosylated	685:698	arg1	SPINK13	N-glycosylated form of SPINK13				OGER		N-glycosylated form of SPINK13	Q1W4C9		Herein we report the chemical synthesis of the scarce N-glycosylated form of SPINK13 by a rapid synthetic method combined with the chemical glycan insertion strategy and a fast-flow SPPS method.
37341462	8	61	gly	O-glycopeptide	1577:1590	arg2	O-glycopeptide			O-glycopeptide						O-glycopeptide	This search mode, the O-Search-Pattern, has been implemented into our database search software, MS-Decipher, and is recommended for searching the O-glycopeptide spectra acquired by sceHCD (stepped collision energy higher-energy collisional dissociation).
35405095	2	34	gly	glycoproteins	383:395	arg1	host glycoproteins	host glycoproteins				Fterm		glycoproteins			The abundance of A. muciniphila is modulated by the presence and accessibility of nutrients, which can be derived from diet or host glycoproteins.
32791164	10	19	gly	glycoprotein	1649:1660	arg1	1				1						Purification and identification revealed deleted in malignant brain tumors 1 (DMBT1) (also known by the aliases GP340 and SALSA), a large glycoprotein with multiple O-glycosylation repeats.
32791164	10	19	gly	glycoprotein	1649:1660	arg1	a large glycoprotein				a large glycoprotein						Purification and identification revealed deleted in malignant brain tumors 1 (DMBT1) (also known by the aliases GP340 and SALSA), a large glycoprotein with multiple O-glycosylation repeats.
36669592	3	44	gly	glycosylation	504:516	arg1	specific proteins	specific proteins				Fterm		proteins			It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation.
35945033	0	41	gly	glycoproteins	193:205	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			PNGase H + variant from Rudaea cellulosilytica with improved deglycosylation efficiency for rapid analysis of eukaryotic N-glycans and hydrogen deuterium exchange mass spectrometry analysis of glycoproteins.
32839225	6	4	gly	sialoglycopeptides	969:986	arg2	N-linked sialoglycopeptides	EL		sialoglycopeptides		Cterm		EL	16891	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg2	N-linked sialoglycopeptides	C4b-binding protein		sialoglycopeptides		PUBTATOR		C4b-binding protein	12269	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg1	testis-expressed protein 101	52, testis-expressed protein 101		sialoglycopeptides		PUBTATOR		52, testis-expressed protein 101	56746	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg1	endothelial lipase	endothelial lipase		sialoglycopeptides		PUBTATOR		endothelial lipase	16891	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg1	zonadhesin	zonadhesin		sialoglycopeptides		PUBTATOR		zonadhesin	Q9Y493	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32791352	1	8	gly	glycoprotein	216:227	arg1	homolog glycoprotein complexes	homolog glycoprotein complexes				Fterm		glycoprotein			Species-specific guinea pig cytomegalovirus (GPCMV) causes congenital CMV and the virus encodes homolog glycoprotein complexes to human CMV, including gH-based trimer (gH/gL/gO) and pentamer-complex (PC).
34878920	11	45	gly	Deglycosylation	1553:1567	arg2	N193			N193				ALV-J Envelope Protein	64006	N193	Deglycosylation at N193 weakened Env-receptor binding while mutation at N17 influenced Env protein processing.
36648436	6	2	gly	α2,6-sialylated	1187:1201	arg1	oligo-mannose, hybrid-type, and α2,6-sialylated structures				oligo-mannose, hybrid-type, and α2,6-sialylated structures						We furthermore show that ZIP9 knockout affects N-linked glycosylation, resulting in up-regulation of oligo-mannose, hybrid-type, and α2,6-sialylated structures as well as down-regulation of tri- and tetra-antennary structures.
36637420	9	32	gly	N-glycosylation	1746:1760	arg1	Asn-57			Asn-57				HAI-2 protein	10653	Asn-57	These results suggest that N-glycosylation on Asn-57 is required for folding into a functional HAI-2 with full protease suppressive activity and correct subcellular targeting signal.
36206692	2	3	gly	Ara	522:524	arg1	Ara, Xyl, Rib, Rha	Ara			Ara, Xyl, Rib, Rha	OGER		Ara	O95255		In this study, a direct acetylation strategy combined with reversed-phase liquid chromatography electrospray tandem multiple reaction monitoring mass spectrometry (RPLC-ESI-MRM-MS) was developed for simultaneous determination of 8 aldoses (Glc, Gal, Man, Ara, Xyl, Rib, Rha and Fuc), a ketose (Fru), 2 alditols (Glc-ol and Man-ol) and 2 uronic acids (GlcA and GalA) on a high-pressure resistant reversed-phase column.
37398203	9	103	gly	-glycosylation	1532:1545	arg2	This novel N -glycosylation site			This novel N -glycosylation site						site	This novel N -glycosylation site (absent in the germline sequence) is a result of a single mutation giving rise to an NDT motif in the antibody sequence.
35925863	9	61	gly	hypersialylated	1378:1392	arg1	the hyperbranched and hypersialylated glycans				the hyperbranched and hypersialylated glycans						Of significance was the hyperbranched and hypersialylated glycans and their derived glycan subclass traits.
35751935	7	27	gly	contained	1037:1045	arg1	The LMP AND arabinose	The LMP			arabinose	OGER		LMP			The LMP extracted by EADU contained arabinose, galactose, and glucose in the molar ratios of 2.9:2.72:5.05.
35751935	7	27	gly	contained	1037:1045	arg1	The LMP AND galactose	The LMP			galactose	OGER		LMP			The LMP extracted by EADU contained arabinose, galactose, and glucose in the molar ratios of 2.9:2.72:5.05.
35751935	7	27	gly	contained	1037:1045	arg1	The LMP AND glucose	The LMP			glucose	OGER		LMP			The LMP extracted by EADU contained arabinose, galactose, and glucose in the molar ratios of 2.9:2.72:5.05.
35091091	5	73	gly	glycopeptide	1242:1253	arg2	complex glycopeptide analyses			complex glycopeptide analyses						glycopeptide	Combined with false discovery rate estimation for the glycan assignment, we show that this method is capable of specifically and sensitively identifying glycans in complex glycopeptide analyses and effectively controls the rate of false glycan assignments.
32187935	4	16	gly	glycosylation	560:572	arg2	the potential N-linked glycosylation sites			the potential N-linked glycosylation sites						sites	NetNGlyc 1.0 server was used to predict the potential N-linked glycosylation sites.
35166741	6	44	gly	glycopeptide	1174:1185	arg2	an improved liquid phase glycopeptide coupling			an improved liquid phase glycopeptide coupling						glycopeptide	To reduce the amount of precious oligosaccharide that is required in the conventional SPPS method, an improved liquid phase glycopeptide coupling was also optimized in a good yield (46% over four steps).
37336068	3	19	gly	glycosylated	444:455	arg1	β-1,4-GlcNAc glycosylated wall teichoic acids				β-1,4-GlcNAc glycosylated wall teichoic acids						β-1,4-GlcNAc glycosylated wall teichoic acids (WTAs) derived from S. aureus are a new type of antigen that is closely associated with β-lactam resistance.
34452239	8	110	gly	glycopeptide	1669:1680	arg2	glycopeptide			glycopeptide						peptide, glycopeptide	The native peptide, glycopeptide, SD, and co-SD powders were comprehensively characterized, and exhibited distinct glass transitions (Tg) consistent with the amorphous glassy state formation with Tgs that are compatible with use in vivo.
36580234	4	17	gly	glycopeptides	549:561	arg2	The intact glycopeptides			The intact glycopeptides						glycopeptides	The intact glycopeptides of macrophages were enriched and analyzed using mass spectrometry (MS)-based glycoproteomic approaches, followed by the large-scale mapping of site-specific glycan structures via StrucGP.
31941975	3	39	gly	fucosylated	500:510	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Although many of biological and clinical studies of protein fucosylation by fucosyltransferases has been reported, structural classification of fucosylated N-glycoproteins such as core or outer isoforms remains a challenge.
31941975	3	96	gly	N-glycoproteins	512:526	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Although many of biological and clinical studies of protein fucosylation by fucosyltransferases has been reported, structural classification of fucosylated N-glycoproteins such as core or outer isoforms remains a challenge.
33075613	4	60	gly	glycoproteins	642:654	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Dose delivery can be impeded by a matrix of N-linked glycoproteins and other polypeptides called the chorion.
35373202	9	34	gly	glycosylation	1837:1849	arg1	this epitope			this epitope						epitope	In addition, in-cell analysis shows that the GalNAc-T4 isoform is the only isoform glycosylating the Thr of the immunogenic epitope PDTRP in vivo, which highlights the relevance of GalNAc-T4 in the glycosylation of this epitope.
35373202	9	55	gly	isoform	1714:1720	arg1	the Thr			the Thr						Thr	In addition, in-cell analysis shows that the GalNAc-T4 isoform is the only isoform glycosylating the Thr of the immunogenic epitope PDTRP in vivo, which highlights the relevance of GalNAc-T4 in the glycosylation of this epitope.
36206406	4	41	gly	modified	677:684	arg3	AlpA/B AND glycans	AlpA/B			glycans	PUBTATOR		AlpA/B	None		Here, we report that key adhesins AlpA/B and BabA/B in <i>H. pylori</i> are modified by glycans and display a two-step molecular weight upshift pattern from the cytoplasm to the inner membrane and from the inner membrane to the outer membrane.
36206406	4	41	gly	modified	677:684	arg1	BabA/B AND glycans	BabA/B			glycans	PUBTATOR		BabA/B	None		Here, we report that key adhesins AlpA/B and BabA/B in <i>H. pylori</i> are modified by glycans and display a two-step molecular weight upshift pattern from the cytoplasm to the inner membrane and from the inner membrane to the outer membrane.
33287410	6	61	gly	trisialylated	1240:1252	arg1	triantennary trigalactosylated trisialylated glycans				triantennary trigalactosylated trisialylated glycans						We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease.
33287410	6	65	gly	monosialylated	1075:1088	arg1	biantennary digalactosylated monosialylated glycans				biantennary digalactosylated monosialylated glycans						We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease.
35380435	8	10	gly	glycopeptide	1731:1742	arg2	glycopeptide data			glycopeptide data						glycopeptide	In addition, our top-down analysis readily captured unexpected modifications (e.g., cysteinylation and N-terminal sequence variation) and low abundance, heavily glycosylated proteoforms that may be missed by using glycopeptide data alone.
36499311	1	47	gly	glycosylated	187:198	arg1	a highly glycosylated enzyme	a highly glycosylated enzyme				Fterm		enzyme			Rhodotorula dairenensis β-fructofuranosidase is a highly glycosylated enzyme with broad substrate specificity that catalyzes the synthesis of 6-kestose and a mixture of the three series of fructooligosaccharides (FOS), fructosylating a variety of carbohydrates and other molecules as alditols.
36499311	1	47	gly	glycosylated	187:198	arg1	Rhodotorula dairenensis β-fructofuranosidase	Rhodotorula dairenensis β-fructofuranosidase				Fterm		β-fructofuranosidase			Rhodotorula dairenensis β-fructofuranosidase is a highly glycosylated enzyme with broad substrate specificity that catalyzes the synthesis of 6-kestose and a mixture of the three series of fructooligosaccharides (FOS), fructosylating a variety of carbohydrates and other molecules as alditols.
35193013	8	51	gly	di-sialylated	1379:1391	arg1	predominantly mono- and di-sialylated N-glycans				predominantly mono- and di-sialylated N-glycans						In BALB/c mouse sera, we detected predominantly mono- and di-sialylated N-glycans with mostly N-Glycolylneuraminic acid (Neu5Gc) and only trace amounts of N-Acetyl neuraminic acid (Neu5Ac).
35995381	1	19	gly	glycosylation	167:179	arg2	many glycosylation sites			many glycosylation sites						sites	Human thyroglobulin (Tg), which has many glycosylation sites, is an essential protein produced by the human thyroid glands.
36029899	9	14	gly	N-glycosylation	1338:1352	arg1	an active ADA2 enzyme	an active ADA2 enzyme				PUBTATOR		ADA2 enzyme	51816		CONCLUSIONS These data suggest that the initial N-glycosylation and N-glycan editing in the ER are essential for the production of an active ADA2 enzyme and proper trafficking to the extracellular space.
36870654	2	13	gly	glycosylation	284:296	arg1	a Family 1 carbohydrate-binding module	glycoprotein			a Family 1 carbohydrate-binding module	Fterm		glycoprotein			The present study addresses this knowledge gap by elucidating the links between the glycosylation patterns of a model glycoprotein, a Family 1 carbohydrate-binding module (TrCBM1), and the thermodynamic and structural properties of its binding to different carbohydrate substrates using isothermal titration calorimetry and computational simulation.
36870654	2	64	gly	glycoprotein	318:329	arg1	a Family 1 carbohydrate-binding module	glycoprotein			a Family 1 carbohydrate-binding module	Fterm		glycoprotein			The present study addresses this knowledge gap by elucidating the links between the glycosylation patterns of a model glycoprotein, a Family 1 carbohydrate-binding module (TrCBM1), and the thermodynamic and structural properties of its binding to different carbohydrate substrates using isothermal titration calorimetry and computational simulation.
34274643	2	44	gly	glycoprotein	374:385	arg1	Human alpha1-acid glycoprotein	Human alpha1-acid glycoprotein				Fterm		glycoprotein			Human alpha1-acid glycoprotein (AGP) was used as a model analyte due to its heterogeneous glycosylation resulting from variations in its degree of branching, fucosylation, and number of sialic acids.
34274643	2	83	gly	fucosylation	514:525	arg1	sialic acids				sialic acids						Human alpha1-acid glycoprotein (AGP) was used as a model analyte due to its heterogeneous glycosylation resulting from variations in its degree of branching, fucosylation, and number of sialic acids.
36968546	7	47	gly	O-glycopeptides	1157:1171	arg2	synthetic O-glycopeptides			synthetic O-glycopeptides						O-glycopeptides	We found that the sole incorporation of N-acetylgalactosamine did not impact furin activity in synthetic O-glycopeptides, but the presence of sialic acid reduced the furin rate by up to 65%.
33552045	3	77	gly	glycosylation	444:456	arg1	viral proteins	proteins		epitopes		Fterm		proteins		epitopes	In this perspective, we hypothesize that glycosylation of viral proteins and processed epitopes contribute to the T cell response to HIV.
36288283	6	28	gly	GPC	1005:1007	arg1	the extensive glycan shield	GPC			the extensive glycan shield	OGER		GPC	P04921		These antibodies either circumvent or exploit specific glycans comprising the extensive glycan shield of GPC.
36252735	1	29	gly	modified	165:172	arg3	The proteins AND glycans	The proteins			glycans	Fterm		proteins			The proteins in the cell membrane of the brain are modified by glycans in highly interactive regions.
36252735	1	30	gly	glycans	177:183	arg1	highly interactive regions			highly interactive regions	highly interactive regions		Site			regions	The proteins in the cell membrane of the brain are modified by glycans in highly interactive regions.
33997890	8	76	gly	glycosylation	1230:1242	arg1	site Thr393			site Thr393				2 (SARS	P49591	site Thr393	The commercial product contained extensive N- and O-linked glycosylation as well as O-phosphorylation on site Thr393.
36324280	1	17	gly	N-glycosylation	181:195	arg2	four N-glycosylation sites			four N-glycosylation sites						sites	Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans.
36324280	1	60	gly	carrying	203:210	arg1	four N-glycosylation sites AND mainly complex type N-glycans			four N-glycosylation sites	mainly complex type N-glycans					sites	Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans.
35481895	7	5	gly	region	1306:1311	arg1	O-glycans			region	O-glycans					region	The S-layer protein is also extensively modified in the threonine-rich region near the C-terminus with O-glycans composed exclusively of hexoses.
35670884	4	4	gly	glycopeptide	779:790	arg2	its VCSNDNcfK glycopeptide			its VCSNDNcfK glycopeptide						glycopeptide	Site-specific mass spectrometry analysis demonstrated that the secreted protein acidic and rich in cysteine (SPARC), which binds collagen, contains a core-fucosylation site in its VCSNDNcfK glycopeptide.
35670884	4	23	gly	site	757:760	arg1	its VCSNDNcfK glycopeptide			its VCSNDNcfK glycopeptide	its VCSNDNcfK glycopeptide		Site			glycopeptide	Site-specific mass spectrometry analysis demonstrated that the secreted protein acidic and rich in cysteine (SPARC), which binds collagen, contains a core-fucosylation site in its VCSNDNcfK glycopeptide.
35670884	4	41	gly	contains	728:735	arg1	SPARC AND a core-fucosylation site	SPARC			a core-fucosylation site	PUBTATOR		SPARC	6678		Site-specific mass spectrometry analysis demonstrated that the secreted protein acidic and rich in cysteine (SPARC), which binds collagen, contains a core-fucosylation site in its VCSNDNcfK glycopeptide.
35484857	4	19	gly	TNF-α	852:856	arg1	the LPS/D-GalN-triggered production	TNF-α, IL-1β,			the LPS/D-GalN-triggered production	PUBTATOR		TNF-α, IL-1β,	7124		HPS-50 significantly decreased the levels of ALT, AST, MPO, and MDA, increased the activities of SOD, CAT, and GSH, and suppressed the LPS/D-GalN-triggered production of TNF-α, IL-1β, and IL-6 (p < .05).
35484857	4	23	gly	IL-1β	859:863	arg1	the LPS/D-GalN-triggered production	TNF-α, IL-1β,			the LPS/D-GalN-triggered production	PUBTATOR		TNF-α, IL-1β,	7124		HPS-50 significantly decreased the levels of ALT, AST, MPO, and MDA, increased the activities of SOD, CAT, and GSH, and suppressed the LPS/D-GalN-triggered production of TNF-α, IL-1β, and IL-6 (p < .05).
35484857	4	58	gly	IL-6	870:873	arg1	the LPS/D-GalN-triggered production	IL-6			the LPS/D-GalN-triggered production	PUBTATOR		IL-6	3569		HPS-50 significantly decreased the levels of ALT, AST, MPO, and MDA, increased the activities of SOD, CAT, and GSH, and suppressed the LPS/D-GalN-triggered production of TNF-α, IL-1β, and IL-6 (p < .05).
35504880	6	35	gly	α-GalNAc-Ser	931:942	arg1	α-GalNAc-Ser peptides			Ser	α-GalNAc-Ser peptides					Ser	By imposing this 3D-arrangement on that fragment, characteristic of α-GalNAc-Ser peptides, C1GalT1 ensures broad glycosylation of both acceptor substrates.
37202422	6	3	gly	afucosylated	884:895	arg1	the afucosylated IgG N-glycan				the afucosylated IgG N-glycan						Upon binding, the elongated CDR3 loop of X0 undergoes a conformational shift to access the buried N-glycan and acts as a 'glycan sensor', forming hydrogen bonds with the afucosylated IgG N-glycan that would otherwise be sterically hindered by the presence of a core fucose residue.
35380345	6	10	gly	sialylated	852:861	arg1	sialylated, fucosylated and mannosylated N-glycans				sialylated, fucosylated and mannosylated N-glycans						The cells grown in HG showed a greater abundance of N-glycans when compared to LNG cells, without changes in the proportion of sialylated, fucosylated and mannosylated N-glycans.
34853076	3	42	gly	glycosylation	374:386	arg2	a large N-linked glycosylation site			a large N-linked glycosylation site						site	Native C5a contains a large N-linked glycosylation site at Asn64, which accounts for up to 25% of its m.w. To date, the vast majority of published studies examining C5a are performed using Escherichia coli-generated recombinant C5a, which is readily available from numerous commercial suppliers, but lacks this glycosylation moiety.
34853076	3	42	gly	glycosylation	374:386	arg2	Asn64			Asn64				Recombinant C5a	728	Asn64	Native C5a contains a large N-linked glycosylation site at Asn64, which accounts for up to 25% of its m.w. To date, the vast majority of published studies examining C5a are performed using Escherichia coli-generated recombinant C5a, which is readily available from numerous commercial suppliers, but lacks this glycosylation moiety.
36181511	4	65	gly	glycopeptide	916:927	arg2	a reasonable glycopeptide enrichment performance			a reasonable glycopeptide enrichment performance						glycopeptide	Exploration of capacity and recovery rate of cotton HILIC illustrated that 5mg cotton packed in a 200μL tip achieved a reasonable glycopeptide enrichment performance (~6% recovery) from ~0.5mg peptides.
35344340	2	20	gly	aglycosylated	497:509	arg1	aglycosylated Fc ADC variants	aglycosylated Fc ADC variants				OGER		Fc ADC variants	Q6ZQY3		We evaluated thermal and metabolic stabilities of antibody-drug conjugates (ADCs) with payloads attached to the C'E loop in the immunoglobulin G (IgG) Fc CH2 domain, comparing the glycosylated and aglycosylated Fc ADC variants.
34985300	2	20	gly	O-glycosylation	261:275	arg2	the O-glycosylation motif			the O-glycosylation motif						motif	O-glycosylation has been reported in this phylum with findings that include the O-glycosylation motif, the structure of the O-glycans in a few species, and an extensive O-glycoproteome analysis in Tannerella forsythia.
35092134	2	67	gly	N-glycosylation	280:294	arg2	11 potential N-glycosylation sites			11 potential N-glycosylation sites						sites	MET contains 11 potential N-glycosylation sites, but the site-specific roles of these N-glycans have not been elucidated.
32602701	5	68	gly	glycosylated	676:687	arg1	Asn-229			Asn-229 and Asn-281				Myonectin	151176	Asn-229 and Asn-281	Mass spectrometry confirmed that Asn-229 and Asn-281 were glycosylated, and substituting both Asn sites with Gln prevented myonectin secretion.
32417172	3	33	gly	glycosylated	401:412	arg1	nucleocytoplasmic proteins	nucleocytoplasmic proteins				Fterm		proteins			However, what nucleocytoplasmic proteins are O-GalNAc glycosylated and what the biological function of this modification in cells are still poorly understood.
31941975	0	19	gly	N-Glycoproteins	59:73	arg1	Outer Fucosylation	N-Glycoproteins			Outer Fucosylation	Fterm		N-Glycoproteins			Machine Learning Classifies Core and Outer Fucosylation of N-Glycoproteins Using Mass Spectrometry.
31941975	0	104	gly	Fucosylation	43:54	arg1	N-Glycoproteins	N-Glycoproteins				Fterm		N-Glycoproteins			Machine Learning Classifies Core and Outer Fucosylation of N-Glycoproteins Using Mass Spectrometry.
36825496	3	18	gly	fucosylated	364:374	arg1	fucosylated fragments			fucosylated fragments						fragments	Here, using ion vibrational spectroscopy, we resolve the structure of fucosylated fragments of Lewis and blood group H antigen trisaccharides and we unveil the position and linkage of the fucose after migration.
36809652	4	26	gly	galectin-1	592:601	arg1	Carbohydrate-dependent binding	galectin-1			Carbohydrate-dependent binding	OGER		galectin-1	P09382		Carbohydrate-dependent binding of galectin-1 to another O-glycoprotein, neuropilin-1 (NRP-1) on endothelial cells activates vascular endothelial growth factor receptor 2 (VEGFR2) and mitogen-activated protein kinase (MAPK) signaling.
36809652	4	95	gly	O-glycoprotein	614:627	arg1	neuropilin-1	neuropilin-1				OGER		neuropilin-1	O14786		Carbohydrate-dependent binding of galectin-1 to another O-glycoprotein, neuropilin-1 (NRP-1) on endothelial cells activates vascular endothelial growth factor receptor 2 (VEGFR2) and mitogen-activated protein kinase (MAPK) signaling.
36809652	4	95	gly	O-glycoprotein	614:627	arg1	another O-glycoprotein	another O-glycoprotein				Fterm		O-glycoprotein			Carbohydrate-dependent binding of galectin-1 to another O-glycoprotein, neuropilin-1 (NRP-1) on endothelial cells activates vascular endothelial growth factor receptor 2 (VEGFR2) and mitogen-activated protein kinase (MAPK) signaling.
34778211	13	32	gly	core-fucosylation	2250:2266	arg1	proteins	proteins				Fterm		proteins			These glycoproteomic results revealed that the knock-out of FUT8 not only influenced the core-fucosylation of proteins but also altered other glycosylation synthesis processes and changed the relative abundance of protein glycosylation.
34778211	13	91	gly	proteins	2271:2278	arg1	the core-fucosylation	proteins			the core-fucosylation	Fterm		proteins			These glycoproteomic results revealed that the knock-out of FUT8 not only influenced the core-fucosylation of proteins but also altered other glycosylation synthesis processes and changed the relative abundance of protein glycosylation.
36181511	2	50	gly	glycopeptide	396:407	arg2	large-scale glycopeptide enrichment			large-scale glycopeptide enrichment						glycopeptide	Herein, we introduced a cotton hydrophilic interaction liquid chromatography (HILIC) approach for large-scale glycopeptide enrichment with 80% acetonitrile/1% trifluoroacetic acid as the optimal sample loading buffer.
36478308	7	82	gly	glycopeptides	1155:1167	arg2	previously detected glycopeptides			previously detected glycopeptides						glycopeptides	Glycopeptide mapping data processing and glycan classification was performed using Genedata Expressionist via a specialised workflow that used libraries of previously detected glycopeptides to greatly reduce processing time.
32636304	1	18	gly	found	279:283	arg2	the surface envelope glycoprotein-120 (gp120) AND the high-mannose glycans	the surface envelope glycoprotein-120 (gp120)			the high-mannose glycans	OGER		gp120	Q14624		N-Linked glycans are critical to the infection cycle of HIV, and most neutralizing antibodies target the high-mannose glycans found on the surface envelope glycoprotein-120 (gp120).
34452239	6	101	gly	glycopeptide	1324:1335	arg2	glycopeptide			glycopeptide						peptide and glycopeptide	Measurements of solubility and lipophilicity of raw Ang (1-7) and raw PNA5 using experimental and computational approaches confirmed that both the peptide and glycopeptide have high-water solubility and are amphipathic.
36131913	8	44	gly	glycans	1547:1553	arg1	Asn162			Asn162	Asn162		AminoAcid	CD16	2214	Asn45 and Asn162, Val158	Furthermore, changes in glycan composition on the receptor have a greater effect for the Val158 variant such that with oligomannose type glycans and with glycans only on Asn45 and Asn162, Val158 becomes the variant with higher affinity to Fc.
36131913	8	44	gly	glycans	1547:1553	arg1	Asn45			Asn45	Asn45		AminoAcid	CD16	2214	Asn45 and Asn162, Val158	Furthermore, changes in glycan composition on the receptor have a greater effect for the Val158 variant such that with oligomannose type glycans and with glycans only on Asn45 and Asn162, Val158 becomes the variant with higher affinity to Fc.
36252443	2	87	gly	glycoproteins	283:295	arg1	Released N-glycans	glycoproteins			Released N-glycans	Fterm		glycoproteins			Released N-glycans from milk glycoproteins act as growth substrates for infant-associated bifidobacteria, which are key members of the breastfed infant's gut.
36493594	0	65	gly	glycoproteins	142:154	arg1	mammalian glycoproteins	mammalian glycoproteins				Fterm		glycoproteins			Peptide-N-glycosidase F or A treatment and procainamide-labeling for identification and quantification of N-glycans in two types of mammalian glycoproteins using UPLC and LC-MS/MS.
36014516	4	11	gly	fragment	758:765	arg1	the polysaccharide fragment Mn				the polysaccharide fragment Mn						Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 106~9.56 × 106 Da, and the FT-IR absorption peak of 892 cm-1 can be used as the quality control markers of SPs.
36014516	4	11	gly	fragment	758:765	arg1	Two monosaccharides				Two monosaccharides						Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 106~9.56 × 106 Da, and the FT-IR absorption peak of 892 cm-1 can be used as the quality control markers of SPs.
36014516	4	75	gly	SPs	886:888	arg1	the polysaccharide fragment Mn	SPs			the polysaccharide fragment Mn	OGER		SPs	P49903		Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 106~9.56 × 106 Da, and the FT-IR absorption peak of 892 cm-1 can be used as the quality control markers of SPs.
36014516	4	75	gly	SPs	886:888	arg1	Two monosaccharides	SPs			Two monosaccharides	OGER		SPs	P49903		Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 106~9.56 × 106 Da, and the FT-IR absorption peak of 892 cm-1 can be used as the quality control markers of SPs.
36068283	3	60	gly	glycopeptides	512:524	arg2	isobaric glycopeptides			isobaric glycopeptides						glycopeptides	Using parallel sequencing of isobaric glycopeptides and isomeric glycans that were separated by reversed-phase and porous graphitic carbon LC, we report a highly sensitive LC MS/MS method for the comprehensive characterization of low-abundance non-human glycans and their closely related structural isomers.
36211377	7	27	gly	sialylated	1195:1204	arg1	sialylated glycans				sialylated glycans						In the follow-up period, the increase in glycans with bisecting GlcNAc and the decrease in sialylated glycans were observed.
35304921	13	63	gly	Glycosylation	1789:1801	arg1	α5 β1 integrin	α5 β1 integrin				Fterm		integrin			SIGNIFICANCE Glycosylation of α5 β1 integrin has been implicated in multiple aspects of integrin function and structure.
36436856	6	16	gly	fucosylated	755:765	arg1	fucosylated N-glycans				fucosylated N-glycans						In GDM milk, fucosylated N-glycans present higher proportion, whereas the proportion of sialylated O-glycans were lower.
36436856	6	41	gly	sialylated	830:839	arg1	sialylated O-glycans				sialylated O-glycans						In GDM milk, fucosylated N-glycans present higher proportion, whereas the proportion of sialylated O-glycans were lower.
35211008	12	36	gly	N-glycosylation	2157:2171	arg1	β1 adrenergic receptors	β1 adrenergic receptors				Fterm		receptors			Decreased N-glycosylation of β1 adrenergic receptors will downregulate the cAMP/PKA signal pathway and inhibit myocardial excitation and contraction coupling.
36959936	2	86	gly	glycoproteins	322:334	arg1	viral glycoproteins	viral glycoproteins				Fterm		glycoproteins			In reality, however, differences in the plant biosynthetic machinery, compared to mammalian cells, can complicate the production of viral glycoproteins.
33176830	4	49	gly	N-glycosylation	635:649	arg2	non-canonical N-glycosylation motifs			non-canonical N-glycosylation motifs						motifs	Here, we explored the functional role of non-canonical N-glycosylation motifs in the conformation N-X-C based on site directed mutagenesis.
34199200	2	25	gly	glycoproteins	582:594	arg1	surface-expressed glycoproteins	surface-expressed glycoproteins				Fterm		glycoproteins			Herein, we investigated the binding of cyanovirin-N (CV-N) to surface-expressed glycoproteins such as those of human immunodeficiency virus (HIV) gp120, hemagglutinin (HA), and Ebola (GP)1,2 and compared their binding affinities with the binding response to the trimer-folded gp140 using surface plasmon resonance (SPR).
35766466	4	48	gly	fucosylated	769:779	arg1	target core fucosylated N-glycans				target core fucosylated N-glycans						Enzymes that have previously been utilized to cleave N-glycans include peptide-N-glycosidase F (PNGase F) to target N-glycans indiscriminately and endoglycosidase F3 (Endo F3) to target core fucosylated N-glycans.
35943155	13	76	gly	oligosaccharides	2176:2191	arg1	cell surface glycoconjugate receptors	receptors			oligosaccharides	Fterm		receptors			IMPORTANCE α-l-Fucosyl residues are frequently present in many relevant glycans, such as human milk oligosaccharides (HMOs), histo-blood group antigens (HBGAs), and epitopes on cell surface glycoconjugate receptors.
37189353	0	45	gly	N-Glycosylation	4:18	arg1	IgG	IgG				Cterm		IgG			The N-Glycosylation of Total Plasma Proteins and IgG in Atrial Fibrillation.
37341462	4	80	gly	O-glycopeptides	791:805	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	In this study, we found that the Y-ion patterns were also frequently observed in the spectra of O-glycopeptides, and a special search approach is presented to identify O-glycopeptides by utilizing the Y-ion patterns.
37341462	4	82	gly	O-glycopeptides	719:733	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	In this study, we found that the Y-ion patterns were also frequently observed in the spectra of O-glycopeptides, and a special search approach is presented to identify O-glycopeptides by utilizing the Y-ion patterns.
36210615	3	61	gly	glycosylation	635:647	arg2	several glycosylation sites			several glycosylation sites						sites	Considering that metalloproteases and serine proteases are abundant components of Bothrops venoms and essential in the envenomation process, and that these enzymes contain several glycosylation sites, the role of sialic acid in venom proteolytic activity was evaluated.
36136114	7	13	gly	glycopeptide	1152:1163	arg2	glycopeptide ionization			glycopeptide ionization						glycopeptide	Using conventional reversed-phase LC-MS methods, the different glycoforms at a given glycosite elute over a narrow retention time window, and glycopeptide ionization is suppressed by co-eluting non-modified peptides.
36136114	7	45	gly	glycoforms	1073:1082	arg2	a given glycosite			a given glycosite						glycosite	Using conventional reversed-phase LC-MS methods, the different glycoforms at a given glycosite elute over a narrow retention time window, and glycopeptide ionization is suppressed by co-eluting non-modified peptides.
36136114	7	48	gly	glycosite	1095:1103	arg2	a given glycosite			a given glycosite						glycosite	Using conventional reversed-phase LC-MS methods, the different glycoforms at a given glycosite elute over a narrow retention time window, and glycopeptide ionization is suppressed by co-eluting non-modified peptides.
35802832	2	13	gly	glycomacropeptide	371:387	arg1	O-glycans			glycomacropeptide	O-glycans					glycomacropeptide	In this study, O-glycans of casein glycomacropeptide from bovine and caprine whey powder were qualitatively and quantitatively analyzed by LC-UV-ESI-MS/MS, and their immune activities and regulatory mechanisms were compared.
37028137	5	66	gly	composition	781:791	arg1	non-reducing termini			non-reducing termini						termini	However, the individual function of the two N-glycans and the effects of N-glycan composition (size, structure, and non-reducing termini) on DET-type reactions are still unclear.
36990248	9	41	gly	T-synthase	1605:1614	arg1	O-glycan profile	T-synthase			O-glycan profile	Fterm		T-synthase			Altogether, our results elucidated the O-glycan profile and function of T-synthase in the silkworm.
34691043	7	4	gly	hyperglycosylated	877:893	arg1	the hyperglycosylated HA trimer	the hyperglycosylated HA trimer				Fterm		trimer			Here, we asked whether cysteine stabilization of the hyperglycosylated HA trimer could reverse this immunodominance by preventing access to the interface epitope and focus responses to the HA receptor binding site (RBS).
36637420	4	0	gly	matriptase	864:873	arg1	complex-type	matriptase			complex-type	OGER		matriptase	P56677		HAI-2 is synthesized with one of two different N-glycan modifications: one of oligomannose-type, which largely remains in the endoplasmic reticulum/Golgi apparatus, and another of complex-type, which is targeted toward the apical surface in vesicle-like structures, and could function as an inhibitor of matriptase and prostasin.
36637420	4	3	gly	prostasin	879:887	arg1	complex-type	prostasin			complex-type	PUBTATOR		prostasin	5652		HAI-2 is synthesized with one of two different N-glycan modifications: one of oligomannose-type, which largely remains in the endoplasmic reticulum/Golgi apparatus, and another of complex-type, which is targeted toward the apical surface in vesicle-like structures, and could function as an inhibitor of matriptase and prostasin.
36399685	3	41	gly	glycoproteins	474:486	arg1	asparagine-linked (N-linked) glycoproteins	asparagine-linked (N-linked) glycoproteins				Fterm		glycoproteins			To address this gap, we developed a set of complementary methods for producing stalled ribosome complexes that displayed asparagine-linked (N-linked) glycoproteins in conformations amenable to downstream functional and glycostructural interrogation.
35276597	0	45	gly	N-glycosylation	30:44	arg1	plant-derived native and recombinant miraculin	plant-derived native and recombinant miraculin				PUBTATOR		miraculin	101255468		Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin.
35931292	7	68	gly	glycosylated	995:1006	arg1	0.41-0.66 μM Trolox/mg protein	0.41-0.66 μM Trolox/mg protein				Fterm		protein			Antioxidant capacity of glycosylated products (0.41-0.66 μM Trolox/mg protein) was markedly greater than that of original protein (0.06 μM Trolox/mg protein) due to the formation of brown polymers with antioxidant activity.
36648436	1	6	gly	sialylated	215:224	arg1	STn	STn			its sialylated form	PUBTATOR		STn	1917		In epithelial cancers, truncated O-glycans, such as the Thomson-nouveau antigen (Tn) and its sialylated form (STn), are up-regulated on the cell surface and associated with poor prognosis and immunological escape.
36181511	1	1	gly	glycopeptides	190:202	arg2	glycopeptides			glycopeptides						glycopeptides	Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples.
36181511	1	10	gly	glycopeptide	91:102	arg2	Selecting proper and efficient glycopeptide enrichment approaches			Selecting proper and efficient glycopeptide enrichment approaches						glycopeptide	Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples.
32357974	7	69	gly	glycoproteins	1463:1475	arg1	misfolded and retrotranslocated glycoproteins	misfolded and retrotranslocated glycoproteins				Fterm		glycoproteins			Chemical inhibition of Peptide:N-Glycanase (PNGase), the endoglycosidase responsible for the removal of glycans from misfolded and retrotranslocated glycoproteins, greatly reduced presentation of this subset of deamidated HLA-bound peptides.
37266972	6	18	gly	glycoprotein	823:834	arg1	glycoprotein B	glycoprotein B				Cterm		glycoprotein B			To this end, endoglycosidase T was co-expressed with an immunoglobulin G or glycoprotein B of human cytomegalovirus in BY-2 cell lines producing only high mannose N-glycans.
36746580	7	28	gly	glycoproteins	1299:1311	arg1	human MFGM glycoproteins	human MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		Meanwhile, the study deepens our understanding of site-specific N-glycosylation of human MFGM glycoproteins.
36746580	7	47	gly	N-glycosylation	1269:1283	arg1	human MFGM glycoproteins	human MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		Meanwhile, the study deepens our understanding of site-specific N-glycosylation of human MFGM glycoproteins.
34985300	13	117	gly	O-glycosylated	2273:2286	arg1	most proteins	most proteins				Fterm		proteins			It was found that most proteins exposed to the periplasm were O-glycosylated; however, the abundant surface exposed proteins were not.
33952641	4	2	gly	glycoproteins	525:537	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			To more globally identify glycoproteins that are differentially expressed on the surface of HHV6A-infected cells, we performed cell surface capture of N-linked glycoproteins present on the surface of T cells infected with HHV6A, and compared these to proteins present on the surface of uninfected T cells.
33952641	4	11	gly	glycoproteins	659:671	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			To more globally identify glycoproteins that are differentially expressed on the surface of HHV6A-infected cells, we performed cell surface capture of N-linked glycoproteins present on the surface of T cells infected with HHV6A, and compared these to proteins present on the surface of uninfected T cells.
36716113	8	106	gly	pro-inflammatory/anti-inflammatory	1792:1825	arg1	more balanced pro-inflammatory/anti-inflammatory glycan profiles			pro	more balanced pro-inflammatory/anti-inflammatory glycan profiles					pro	CONCLUSION Patients with antibiotic-responsive LA had Bb-IgG1 antibodies with more balanced pro-inflammatory/anti-inflammatory glycan profiles, whereas patients with antibiotic-refractory LA had Bb-IgG1 antibodies with maximal, minimally opposed, pro-inflammatory glycan profiles.
36716113	8	126	gly	maximal	1919:1925	arg1	pro-inflammatory			pro-inflammatory						pro	CONCLUSION Patients with antibiotic-responsive LA had Bb-IgG1 antibodies with more balanced pro-inflammatory/anti-inflammatory glycan profiles, whereas patients with antibiotic-refractory LA had Bb-IgG1 antibodies with maximal, minimally opposed, pro-inflammatory glycan profiles.
36049519	9	61	gly	glycosylated	1478:1489	arg1	the glycosylated residue			the glycosylated residue						residue	The enzyme catalyzes hydrolysis of the bond immediately N-terminal to the glycosylated residue.
35670884	0	7	gly	core-fucosylation	8:24	arg1	SPARC	SPARC				PUBTATOR		SPARC	6678		Loss of core-fucosylation of SPARC impairs collagen binding and contributes to COPD.
35670884	0	52	gly	SPARC	29:33	arg1	core-fucosylation	SPARC			core-fucosylation	PUBTATOR		SPARC	6678		Loss of core-fucosylation of SPARC impairs collagen binding and contributes to COPD.
31941975	10	57	gly	fucosylated	1877:1887	arg1	fucosylated N-glycopeptides			fucosylated N-glycopeptides						N-glycopeptides	Thus, the machine learning methods can be combined with MS/MS to distinguish between different isoforms of fucosylated N-glycopeptides.
31941975	10	106	gly	N-glycopeptides	1889:1903	arg2	fucosylated N-glycopeptides			fucosylated N-glycopeptides						N-glycopeptides	Thus, the machine learning methods can be combined with MS/MS to distinguish between different isoforms of fucosylated N-glycopeptides.
31657490	7	34	gly	glycopeptides	1233:1245	arg2	glycopeptides			glycopeptides						glycopeptides	In addition, the proposed material showed good performance in the enrichment of glycopeptides from complex biosamples; 56 glycopeptides were detected from 2 μL of human serum using MALDI-TOFMS.
31657490	7	87	gly	glycopeptides	1275:1287	arg2	56 glycopeptides			56 glycopeptides						glycopeptides	In addition, the proposed material showed good performance in the enrichment of glycopeptides from complex biosamples; 56 glycopeptides were detected from 2 μL of human serum using MALDI-TOFMS.
35848837	3	51	gly	glycopeptides	467:479	arg2	glycopeptides			glycopeptides						glycopeptides	A common method for glycan analysis is to employ exoglycosidase cleavage to release N-linked glycans from glycoproteins or glycopeptides using Peptide-N-Glycosidase F (PNGase F).
35848837	3	58	gly	glycoproteins	450:462	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A common method for glycan analysis is to employ exoglycosidase cleavage to release N-linked glycans from glycoproteins or glycopeptides using Peptide-N-Glycosidase F (PNGase F).
37331167	8	40	gly	epitope	1212:1218	arg1	α-1,3 mannose				α-1,3 mannose						These results demonstrated that α-1,3 mannose was an critical component of glycan related epitope.
36136114	6	7	gly	glycoprotein	980:991	arg1	glycoprotein quality control	glycoprotein quality control				Fterm		glycoprotein			Because of the inherent heterogeneity of glycosylation, it is necessary to assign glycan structural detail for glycoprotein quality control.
36585837	13	56	gly	glycosylated	1766:1777	arg1	glycosylated milk proteins	glycosylated milk proteins				Fterm		proteins			These data are described and the relevance of glycosylated milk proteins in neonate development, such as protection against pathogens is discussed.
35219398	5	48	gly	glycosylated	809:820	arg1	minimally glycosylated CCM1	minimally glycosylated CCM1				PUBTATOR		CCM1	889		We present the first crystal structure of minimally glycosylated CCM1 in the GFCC'C″ dimer conformation and characterization in solution by continuous-wave and double electron-electron resonance electron paramagnetic resonance spectroscopy.
37341462	7	51	gly	glycopeptide	1335:1346	arg2	15.4%-199.0% more O-glycopeptide-spectrum matches (OGPSMs) and 19.6%-107.1% more glycopeptide sequence identifications			15.4%-199.0% more O-glycopeptide-spectrum matches (OGPSMs) and 19.6%-107.1% more glycopeptide sequence identifications						glycopeptide	The new search strategy was applied to search a human serum data set, and 15.4%-199.0% more O-glycopeptide-spectrum matches (OGPSMs) and 19.6%-107.1% more glycopeptide sequence identifications than other state-of-the-art software tools were observed.
32691273	2	113	gly	residues	405:412	arg1	the GlcNAc molecules			threonine residues	the GlcNAc molecules					threonine residues	O-GlcNAc transferase (OGT) catalyzes the attachment, while O-GlcNAcase (OGA) splits the GlcNAc molecules from the serine or threonine residues of the nuclear and cellular proteins.
36925056	4	39	gly	monosialylated	912:925	arg1	digalactosylated, monosialylated, and antennary fucosylated derived traits	digalactosylated, monosialylated, and antennary fucosylated derived traits				OGER		traits	Q96CJ1		RESULTS Compared to healthy controls, subjects with type 1 diabetes showed differences in 19 glycan groups and a decrease in monogalactosylated, an increase in digalactosylated, monosialylated, and antennary fucosylated derived traits, from which changes in monogalactosylation and seven directly measured traits overlapped with previously reported in children.
36056620	6	60	gly	glycosylation	1110:1122	arg2	N6 glycosylation motifs			N6 glycosylation motifs						motifs	Further reverse genetic studies of acquired E1/E2 substitutions identified positions 418 and 532 in the N1 and N6 glycosylation motifs, localizing to adjacent E2 areas, as key regulators of changes of the E1/E2 conformational state, which governed viral sensitivity to nAb.
37287875	0	6	gly	N-glycopeptides	19:33	arg2	intact N-glycopeptides			intact N-glycopeptides						N-glycopeptides	Identifying intact N-glycopeptides from tandem mass spectrometry data using StrucGP.
36813234	8	64	gly	contains	1455:1462	arg1	TNFR2 AND bisected N-glycans	TNFR2			bisected N-glycans	OGER		TNFR2	P20333		Interestingly, our immunoprecipitation analysis revealed that only TNFR2, but not TNFR1, contains bisected N-glycans.
36813234	8	64	gly	contains	1455:1462	arg1	TNFR1 AND bisected N-glycans	TNFR1			bisected N-glycans	OGER		TNFR1	P19438		Interestingly, our immunoprecipitation analysis revealed that only TNFR2, but not TNFR1, contains bisected N-glycans.
34012659	10	73	gly	non-N-glycosylated	1195:1212	arg1	aberrant non-N-glycosylated BST-2	aberrant non-N-glycosylated BST-2				PUBTATOR		BST-2	684		RESULTS Here, we observed the higher BST-2 expression in HBV-infected HCC than their paired adjacent tissues and HBV-uninfected HCC tissues, particularly more aberrant non-N-glycosylated BST-2 in HBV-infected HCC tumors.
36329887	1	70	gly	glycoprotein	194:205	arg1	an α/β heterodimeric glycoprotein hormone	an α/β heterodimeric glycoprotein hormone				Fterm		glycoprotein			Follicle-stimulating hormone (FSH), an α/β heterodimeric glycoprotein hormone, consists of functionally significant variants resulting from the presence or absence of either one of two FSHβ subunit N-glycans.
36269466	10	14	gly	glycoproteins	1495:1507	arg1	misfolded glycoproteins	misfolded glycoproteins				Fterm		glycoproteins			This hints at the accumulation of misfolded glycoproteins in the infected plants, likely because of endoplasmic reticulum (ER) stress.
35871410	8	89	gly	O-glycan	1293:1300	arg1	the Δmnt1 strain	strain			O-glycan	Fterm		strain			In this study, we elucidated the structure of the Mnt1 reaction product, the structure of O-glycan in the Δmnt1 strain.
36580234	7	53	gly	glycosylated	1064:1075	arg1	glycosylated PD-L1	glycosylated PD-L1				PUBTATOR		PD-L1	29126		In addition, a high level of glycosylated PD-L1 was observed in M1 macrophages, and the LacNAc moiety was detected at Asn-192 and Asn-200 of PD-L1, and Asn-200 contained Lewis epitopes.
31657490	2	29	gly	glycopeptides	416:428	arg2	glycopeptides			glycopeptides						glycopeptides	Therefore, selective enrichment of glycopeptides before mass spectrometry has turned into an urgent problem to be resolved.
37316505	0	57	gly	glycosylation	48:60	arg1	the stalk region			region						region	Intact mass analysis reveals the novel O-linked glycosylation on the stalk region of PD-1 protein.
34864901	0	0	gly	proteins	59:66	arg1	glycan shielding	proteins			glycan shielding	Fterm		proteins			GLYCO: a tool to quantify glycan shielding of glycosylated proteins.
34864901	0	5	gly	glycosylated	46:57	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			GLYCO: a tool to quantify glycan shielding of glycosylated proteins.
36233110	4	56	gly	N-glycosylation	923:937	arg2	all five N-glycosylation sites			all five N-glycosylation sites						sites	Three aflibercept lots showed a high degree of similarity in glycan composition, fucosylation level, sialylation level, and branching, when all five N-glycosylation sites were assessed together as a group.
35598016	2	50	gly	machinery	391:399	arg1	the thermophilic Caldicoprobacter algeriensis TH7C1T strain	strain			machinery	Fterm		strain			The aim of this study was to identify protein encoding genes including CAZymes in order to understand glycans-degrading machinery in the thermophilic Caldicoprobacter algeriensis TH7C1T strain.
36089065	0	40	gly	N-glycoprotein	30:43	arg1	Sequential in vitro enzymatic N-glycoprotein modification	Sequential in vitro enzymatic N-glycoprotein modification				Fterm		N-glycoprotein			Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing.
36827096	4	9	gly	glycoprotein	603:614	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			GYL is a glycoprotein containing two N-glycosylation sites per subunit.
36827096	4	56	gly	N-glycosylation	631:645	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	GYL is a glycoprotein containing two N-glycosylation sites per subunit.
37258966	1	22	gly	glycoproteins	320:332	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycosylation is a common posttranslational modification of proteins and refers to the covalent addition of glycans, chains of polysaccharides, onto proteins producing glycoproteins.
34735575	1	16	gly	sites	343:347	arg1	glycans			sites	glycans					sites	Extensive glycosylation of the spike protein of severe acute respiratory syndrome coronavirus 2 virus not only shields the major part of it from host immune responses, but glycans at specific sites also act on its conformation dynamics and contribute to efficient host receptor binding, and hence infectivity.
34735575	1	21	gly	glycosylation	161:173	arg1	the spike protein	the spike protein				PUBTATOR		spike protein	43740568		Extensive glycosylation of the spike protein of severe acute respiratory syndrome coronavirus 2 virus not only shields the major part of it from host immune responses, but glycans at specific sites also act on its conformation dynamics and contribute to efficient host receptor binding, and hence infectivity.
35495330	6	75	gly	glycosylation	1457:1469	arg2	a naturally found N-linked glycosylation site			a naturally found N-linked glycosylation site						site	Thus, mono- and dimannosylated peptides with N-terminal cysteine facilitated site-specific interactions with HA peptides, mimicking a naturally found N-linked glycosylation site on the HA head domain.
35495330	6	88	gly	dimannosylated	1314:1327	arg1	mono- and dimannosylated peptides			mono- and dimannosylated peptides						peptides	Thus, mono- and dimannosylated peptides with N-terminal cysteine facilitated site-specific interactions with HA peptides, mimicking a naturally found N-linked glycosylation site on the HA head domain.
36385894	10	60	gly	glycopeptide	1960:1971	arg2	glycopeptide biomarkers			glycopeptide biomarkers						glycopeptide	This report represents the first serum glycan and glycopeptide biomarkers of Philippine lung cancer patients, further demonstrating the utility of mass spectrometry-based glycomic and glycoproteomic methods.
34726173	4	18	gly	glycosylated	1104:1115	arg1	glycosylated SDR peptides			glycosylated SDR peptides						peptides	This study reports the crystal structures of SdgB and SdgA from S. aureus as well as multiple structures of SdgB in complex with its substrates (for example UDP, N-acetylglucosamine or SDR peptides), products (glycosylated SDR peptides) or phosphate ions.
36988338	5	7	gly	proteins	737:744	arg1	polysaccharides	proteins			polysaccharides	Fterm		proteins			Although the functional groups of polysaccharides and proteins were confirmed by infrared spectroscopy; the presence of proteins couldn't be detected by SDS-PAGE and UV spectroscopy.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	several glycoproteins	several glycoproteins				Fterm		glycoproteins			The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	metalloprotease	metalloprotease				Fterm		metalloprotease			The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	serine protease	serine protease				Fterm		protease			The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	L- amino acid oxidase	L- amino acid oxidase				OGER		L- amino acid oxidase	Q96RQ9		The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
36903897	1	56	gly	glycoprotein	197:208	arg1	the hydroxyproline-rich glycoprotein (HRGP) superfamily	the hydroxyproline-rich glycoprotein (HRGP) superfamily				Fterm		glycoprotein			Arabinogalactan-proteins (AGPs) are members of the hydroxyproline-rich glycoprotein (HRGP) superfamily.
36585837	10	32	gly	glycosylation	1449:1461	arg2	glycosylation sites			glycosylation sites						sites	Glycoproteins were found across all three phases of swine milk production and varied by number of glycosylation sites (1 to 14) and in abundance and distribution between colostrum, transitional and mature milk.
35987426	3	96	gly	glycoprotein	485:496	arg1	the glycoprotein pattern	the glycoprotein pattern				Fterm		glycoprotein			The aim of the present study was to examine the glycoprotein pattern of the horse urinary bladder urothelium by lectin histochemistry.
35865907	2	95	gly	polysaccharides	482:496	arg1	shorter sequences			shorter sequences	shorter sequences		Site			sequences	Reported here is an efficient, stereoselective and orthogonal one-pot synthesis of a tetradecasaccharide and shorter sequences from Lentinus giganteus polysaccharides with antitumor activities.
35166741	1	29	gly	glycoproteins	246:258	arg1	natural glycoproteins	natural glycoproteins				Fterm		glycoproteins			The synthesis of a sufficient amount of homogeneous glycoprotein is of great interest because natural glycoproteins show considerable heterogeneity in oligosaccharide structures, making the studies on glycan structure-function relationship difficult.
35166741	1	51	gly	glycoprotein	196:207	arg1	homogeneous glycoprotein	homogeneous glycoprotein				Fterm		glycoprotein			The synthesis of a sufficient amount of homogeneous glycoprotein is of great interest because natural glycoproteins show considerable heterogeneity in oligosaccharide structures, making the studies on glycan structure-function relationship difficult.
36014368	8	6	gly	N-glycosylation	1217:1231	arg2	the 22 predicted N-glycosylation sites			the 22 predicted N-glycosylation sites						sites	We show that 20 among the 22 predicted N-glycosylation sites are dominated by complex plant N-glycans and one carries oligomannoses.
36870092	7	8	gly	N-glycopeptides	935:949	arg2	The intact N-glycopeptides			The intact N-glycopeptides						N-glycopeptides	The intact N-glycopeptides and differentially expressed intact N-glycopeptides (DEGPs) were determined and quantified via intact N-glycopeptide search engine GPSeeker.
36870092	7	56	gly	N-glycopeptides	987:1001	arg2	differentially expressed intact N-glycopeptides			differentially expressed intact N-glycopeptides						N-glycopeptides	The intact N-glycopeptides and differentially expressed intact N-glycopeptides (DEGPs) were determined and quantified via intact N-glycopeptide search engine GPSeeker.
36870092	7	75	gly	N-glycopeptide	1053:1066	arg2	intact N-glycopeptide search engine			intact N-glycopeptide search engine						N-glycopeptide	The intact N-glycopeptides and differentially expressed intact N-glycopeptides (DEGPs) were determined and quantified via intact N-glycopeptide search engine GPSeeker.
36781790	1	36	gly	glycopeptides	233:245	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Mass spectrometry-driven glycomics and glycoproteomics, the system-wide profiling of detached glycans and intact glycopeptides from biological samples, respectively, are powerful approaches to interrogate the heterogenous glycoproteome.
35945033	4	118	gly	glycoproteins	1130:1142	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Furthermore, liquid chromatography-mass spectrometry (LC-MS) workflows, such as hydrogen deuterium exchange mass spectrometry (HDX-MS), require a highly efficient enzymatic release of N-glycans at low pH values to facilitate the comprehensive structural analysis of glycoproteins.
37354227	5	16	gly	glycosylation	1008:1020	arg1	the spike protein	protein		sites		Fterm		protein		sites	Our data reveal that the amino acid substitutions and deletions between variants impact the abundance and type of glycans on glycosylation sites of the spike protein.
36385894	1	80	gly	glycosylation	239:251	arg1	secreted proteins	secreted proteins				Fterm		proteins			Aberrant glycosylation has been extensively reported in cancer, with fundamental changes in the glycosylation patterns of cell-surface and secreted proteins largely occurring during cancer progression.
36775128	6	20	gly	glycosylation	835:847	arg1	these proteins	these proteins				Fterm		proteins			We examine the glycosylation states of these proteins using a combination of antibody-enabled and ZIC-HILIC enrichment techniques, to demonstrate that these proteins are modified with C-, O-, and N-linked glycans.
36775128	6	43	gly	modified	990:997	arg1	these proteins AND N-linked glycans	these proteins			N-linked glycans	Fterm		proteins			We examine the glycosylation states of these proteins using a combination of antibody-enabled and ZIC-HILIC enrichment techniques, to demonstrate that these proteins are modified with C-, O-, and N-linked glycans.
33161144	1	25	gly	glycoproteins	214:226	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			The N-linked glycosylation pattern is an important quality attribute of therapeutic glycoproteins.
36985724	4	2	gly	glycoprotein	848:859	arg1	in vitro processing glycoprotein N-glycans				in vitro processing glycoprotein N-glycans						Toward this goal, we have successfully expressed in E. coli glycoside hydrolases and glycosyltransferases from bacterial and human origins and developed a robust enzymatic platform for in vitro processing glycoprotein N-glycans from high-mannose-type to α2-6- or α2-3-disialylated biantennary complex type.
36985724	4	42	gly	α2-3-disialylated	906:922	arg1	α2-3-disialylated biantennary complex type				α2-3-disialylated biantennary complex type						Toward this goal, we have successfully expressed in E. coli glycoside hydrolases and glycosyltransferases from bacterial and human origins and developed a robust enzymatic platform for in vitro processing glycoprotein N-glycans from high-mannose-type to α2-6- or α2-3-disialylated biantennary complex type.
36135182	3	14	gly	N-glycosylation	374:388	arg2	the N-glycosylation sites			the N-glycosylation sites						sites	By mutating the N-glycosylation sites, we aimed to study the effect of its N-glycan chain on plasminogen activation, fibrin sensitivity, and to observe the physicochemical properties of DSPAα2.
35495330	1	21	gly	glycoproteins	297:309	arg1	various glycoproteins	various glycoproteins				Fterm		glycoproteins			Cyanovirin-N (CV-N) has been shown to reveal broad neutralizing activity against human immunodeficiency virus (HIV) and to specifically bind Manα(1→2)Manα units exposed on various glycoproteins of enveloped viruses, such as influenza hemagglutinin (HA) and Ebola glycoprotein.
35495330	1	89	gly	glycoprotein	380:391	arg1	Ebola glycoprotein	Ebola glycoprotein				Fterm		glycoprotein			Cyanovirin-N (CV-N) has been shown to reveal broad neutralizing activity against human immunodeficiency virus (HIV) and to specifically bind Manα(1→2)Manα units exposed on various glycoproteins of enveloped viruses, such as influenza hemagglutinin (HA) and Ebola glycoprotein.
33316265	8	70	gly	eNOS	1277:1280	arg1	O-GlcNAcylation	eNOS			O-GlcNAcylation	PUBTATOR		eNOS	24600		O-GlcNAcylation of eNOS decreased in P-SHR compared to NP-SHR.
36746580	2	85	gly	N-glycosylation	350:364	arg1	human MFGM proteins	human MFGM proteins				OGER		MFGM proteins	Q08431		However, the profiles and landscape changes in the site-specific N-glycosylation of human MFGM proteins during lactation remain unclear.
37382290	2	6	gly	glycopeptide	267:278	arg2	isobaric glycopeptide structures			isobaric glycopeptide structures						glycopeptide	Despite its immense potential, the qualitative and quantitative analysis of isobaric glycopeptide structures remains one of the most daunting hurdles in the field of glycoproteomics.
37040463	4	10	gly	glycoproteins	502:514	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Cell-free protein synthesis systems have matured as a promising approach for making glycoproteins that may overcome current limitations and enable new glycoprotein medicines.
37040463	4	34	gly	glycoprotein	569:580	arg1	new glycoprotein medicines	new glycoprotein medicines				Fterm		glycoprotein			Cell-free protein synthesis systems have matured as a promising approach for making glycoproteins that may overcome current limitations and enable new glycoprotein medicines.
36585837	4	24	gly	glycosylated	439:450	arg1	N -linked glycosylated proteins	N -linked glycosylated proteins				Fterm		proteins			Our objective was to characterize N -linked glycosylated proteins in homogenate swine milk samples collected from sows (n=5/6) during farrowing to represent colostrum, and on day 3 and 14 post-farrowing to represent transitional and mature milk, respectively.
35670884	9	27	gly	fucosylation	1407:1418	arg1	SPARC	SPARC				PUBTATOR		SPARC	6678		These findings provide a new mechanistic insight into the role of core fucosylation of SPARC in cell-matrix communication and contribution to the abnormal alveolar structures in COPD.
35670884	9	38	gly	SPARC	1423:1427	arg1	core fucosylation	SPARC			core fucosylation	PUBTATOR		SPARC	6678		These findings provide a new mechanistic insight into the role of core fucosylation of SPARC in cell-matrix communication and contribution to the abnormal alveolar structures in COPD.
35586945	6	20	gly	N-glycosylation	1094:1108	arg2	specific N-glycosylation sites			specific N-glycosylation sites						sites	Moreover, distinct types of N-glycans on specific N-glycosylation sites regulate DMA-1/LRR-TM receptor function, which, together with three other extracellular proteins, forms the Menorin adhesion complex.
35586945	6	54	gly	N-glycans	1072:1080	arg1	specific N-glycosylation sites			specific N-glycosylation sites	specific N-glycosylation sites		Site			sites	Moreover, distinct types of N-glycans on specific N-glycosylation sites regulate DMA-1/LRR-TM receptor function, which, together with three other extracellular proteins, forms the Menorin adhesion complex.
37026711	6	7	gly	glycosylation	1251:1263	arg2	each glycosylation site			each glycosylation site						site	We modeled four human SLC6 transporters, the serotonin, dopamine, glycine, and B0AT1 transporters, by first simulating all possible combinations of a glycan attached to each glycosylation site followed by investigating the effect of larger, oligo-N-linked glycans to each transporter.
37026711	6	38	gly	attached	1234:1241	arg2	each glycosylation site AND a glycan			each glycosylation site	a glycan					site	We modeled four human SLC6 transporters, the serotonin, dopamine, glycine, and B0AT1 transporters, by first simulating all possible combinations of a glycan attached to each glycosylation site followed by investigating the effect of larger, oligo-N-linked glycans to each transporter.
37060669	0	48	gly	fucosylated	39:49	arg1	a novel fucosylated trisaccharide				a novel fucosylated trisaccharide						Structural characterization of a novel fucosylated trisaccharide prepared from bacterial exopolysaccharides and evaluation of its prebiotic activity.
36565355	7	39	gly	EGF27	818:822	arg1	the O-fucose site	EGF27			the O-fucose site	Cterm		EGF27	13645		It has been shown that the loss of the O-fucose site of EGF27 alters NOTCH1 activity.
35504880	5	47	gly	α-GalNAc-Thr	807:818	arg1	the α-GalNAc-Thr linkage			Thr	the α-GalNAc-Thr linkage					Thr	Interestingly, to achieve glycosylation, C1GalT1 recognizes a high-energy conformation of the α-GalNAc-Thr linkage, negligibly populated in solution.
36192063	7	48	gly	N-glycoproteins	1426:1440	arg1	Eight intact N-glycoproteins	Eight intact N-glycoproteins				Fterm		N-glycoproteins			Eight intact N-glycoproteins responsible for N-glycan biosynthesis were identified as glycosyltransferases.
32691273	11	115	gly	proteins	1628:1635	arg1	elevated O-GlcNAcylation	proteins			elevated O-GlcNAcylation	Fterm		proteins			We found that elevated O-GlcNAcylation of the total proteins displayed a definite correlation in insulin resistance and endoplasmic reticulum stress.
34276694	0	100	gly	Glycosylation	31:43	arg1	Interleukin-7	Interleukin-7				PUBTATOR		Interleukin-7	3574		Internal Disulfide Bonding and Glycosylation of Interleukin-7 Protect Against Proteolytic Inactivation by Neutrophil Metalloproteinases and Serine Proteases.
36233110	7	24	gly	N-glycosylation	1553:1567	arg2	multiple N-glycosylation sites			multiple N-glycosylation sites						sites	These results imply that analyses of the glycosylation profiles of fusion proteins containing multiple N-glycosylation sites, such as aflibercept, being done as a part of quality control for the therapeutics manufacturing process or for biosimilar development, can be done with a more applicable outcome by assessing each site separately.
36233110	7	67	gly	glycosylation	1491:1503	arg1	fusion proteins	fusion proteins				Fterm		proteins			These results imply that analyses of the glycosylation profiles of fusion proteins containing multiple N-glycosylation sites, such as aflibercept, being done as a part of quality control for the therapeutics manufacturing process or for biosimilar development, can be done with a more applicable outcome by assessing each site separately.
35887202	7	6	gly	O-glycosylated	1094:1107	arg1	the O-glycosylated Thr			the O-glycosylated Thr						Thr	MD simulations of MUC1 glycopeptide showed that the O-glycosylation significantly limits the flexibility of the peptide backbone and side chain of the O-glycosylated Thr.
35887202	7	45	gly	glycopeptide	966:977	arg2	MUC1 glycopeptide			MUC1 glycopeptide						glycopeptide	MD simulations of MUC1 glycopeptide showed that the O-glycosylation significantly limits the flexibility of the peptide backbone and side chain of the O-glycosylated Thr.
36894060	10	59	gly	alte	1335:1338	arg1	polysaccharides	alte			polysaccharides	OGER		alte	O96006		These results will provide insights into specific structures and functions of polysaccharides from the V. alte.
35995210	2	11	gly	N-glycosylated	268:281	arg1	TS enzymes	TS enzymes				Fterm		enzymes			TS enzymes are N-glycosylated, but the biological functions of their glycans have remained elusive.
34726173	2	1	gly	glycosylation	296:308	arg1	the long-chain SDR domain			the long-chain SDR domain						domain	The O-linked glycosylation of the long-chain SDR domain mediated by SdgB and SdgA is a key virulence factor that protects the adhesive SDR proteins against host proteolytic attack in order to promote successful tissue colonization, and has also been implicated in staphylococcal agglutination, which leads to sepsis and an immunodominant epitope for a strong antibody response.
36972173	5	23	gly	present	682:688	arg1	SARS-CoV-2 AND the 22 N-linked glycan attachment sites	SARS-CoV-2			the 22 N-linked glycan attachment sites	OGER		SARS	P49591		Of the 22 N-linked glycan attachment sites present on SARS-CoV-2, 15 are shared by all 12 sarbecoviruses.
34957216	7	43	gly	glycoproteins	1163:1175	arg1	their S glycoproteins	their S glycoproteins				PUBTATOR		S glycoproteins	43740568		For example, G839W of SARS-CoV-1 corresponds to G857W of SARS-CoV-2, which decrease the stability of their S glycoproteins.
37354227	4	58	gly	N-glycosylation	664:678	arg1	recombinant spike proteins	recombinant spike proteins				Fterm		proteins			In this report, we conducted mass spectrometric analysis of the N-glycosylation profiles of recombinant spike proteins derived from four common SARS-CoV-2 variants classified as Variant of Concern, including Alpha, Beta, Gamma, and Delta along with D614G variant spike as a control.
35166741	2	6	gly	glycoproteins	484:496	arg1	homogeneous glycoproteins	homogeneous glycoproteins				Fterm		glycoproteins			Herein, we report optimized methods that can accelerate the semisynthesis of homogeneous glycoproteins based on recombinant expression and chemical conversion.
36266689	0	20	gly	N-glycoproteins	118:132	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			Endoplasmic reticulum-quality control pathway and endoplasmic reticulum-associated degradation mechanism regulate the N-glycoproteins and N-glycan structures in the diatom Phaeodactylum tricornutum.
36838558	3	44	gly	glycans	421:427	arg1	the Gc protein	protein			glycans	Fterm		protein			GcMAF is produced when glycans on the Gc protein are hydrolyzed by α-Sia-ase and β-Gal-ase, leaving an α-GalNAc.
32340215	2	51	gly	glycoprotein	285:296	arg1	a glycoprotein member	a glycoprotein member				Fterm		glycoprotein			MAG is a glycoprotein member of the immunoglobulin superfamily, expressed by myelination cells.
34972858	1	2	gly	glycoproteins	193:205	arg1	biotherapeutic glycoproteins	biotherapeutic glycoproteins				Fterm		glycoproteins			The glycosylation profile of biotherapeutic glycoproteins is a critical quality attribute that is routinely monitored to ensure desired product quality, safety and efficacy.
34972858	1	53	gly	glycosylation	153:165	arg1	biotherapeutic glycoproteins	biotherapeutic glycoproteins				Fterm		glycoproteins			The glycosylation profile of biotherapeutic glycoproteins is a critical quality attribute that is routinely monitored to ensure desired product quality, safety and efficacy.
36252443	9	14	gly	glycoproteins	1486:1498	arg1	native glycoproteins	native glycoproteins				Fterm		glycoproteins			The kinetic evaluation showed that EndoBI-1 displayed higher activity on native glycoproteins than PNGase F, with 0.036 mg/mL×min and 0.012 mg/mL×min glycan release, respectively.
35413075	0	23	gly	fucosylated	73:83	arg1	fucosylated structures				fucosylated structures						Increased levels of acidic free-N-glycans, including multi-antennary and fucosylated structures, in the urine of cancer patients.
36754227	2	7	gly	glycosylation	235:247	arg1	position N573			position						position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	9	gly	glycosylation	322:334	arg1	position N559			position						position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
34154738	3	9	gly	FUT	560:562	arg1	2- fluoro peracetylated fucose	FUT			2- fluoro peracetylated fucose	PUBTATOR		FUT	100689278		The first method uses a fucosyltransferase (FUT) inhibitor, 2- fluoro peracetylated fucose (2FF), which was added to cell cultures expressing the EG2-hFc antibody in increasing concentrations up to 50μM.
35022400	0	39	gly	glycoproteins	16:28	arg1	Mammalian brain glycoproteins				Mammalian brain glycoproteins						Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues.
36598201	5	42	gly	glycosylation	713:725	arg2	one glycosylation site			one glycosylation site						site	The growth rate of recombinant viruses that lost one glycosylation site was as high as that of the wild-type virus.
36329887	2	38	gly	fully-glycosylated	381:398	arg1	The two most abundant variants	The two most abundant variants				Fterm		variants			The two most abundant variants are fully-glycosylated FSH24 (based on 24 kDa FSHβ band in Western blots) and hypo-glycosylated FSH21 (21 kDa band, lacks βAsn<sup>24</sup> glycans).
35678155	3	63	gly	had	725:727	arg1	APS2 AND galacturonic acid	APS2			galacturonic acid	OGER		APS2	Q8NFP7		Results indicated that the two kinds of APS had the same monomer compositions in different molar proportions, where APS2 had greater content of arabinose and galacturonic acid than APS1.
36095241	4	55	gly	unglycosylated	824:837	arg1	the unglycosylated peptide			the unglycosylated peptide						peptide	Compared with the unglycosylated peptide, the binding of sT-P320-334 to hACE2 was enhanced to 133% and the inhibition capacity against RBD-hACE2 binding of sTn- and sT-P320-334 was significantly increased up to 150-410%.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	relaxation				relaxation						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	contraction				contraction						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	N-glycosylation				N-glycosylation						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	calcium transient				calcium transient						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
37319314	1	20	gly	compositions	295:306	arg1	specific sites			specific sites	specific sites		Site			sites	Glycosylation is an essential protein modification occurring on the majority of extracellular human proteins, with mass spectrometry (MS) being an indispensable tool for its analysis, that not only determines glycan compositions, but also the position of the glycan at specific sites via glycoproteomics.
37319314	1	23	gly	sites	357:361	arg1	glycan compositions			sites	glycan compositions					sites	Glycosylation is an essential protein modification occurring on the majority of extracellular human proteins, with mass spectrometry (MS) being an indispensable tool for its analysis, that not only determines glycan compositions, but also the position of the glycan at specific sites via glycoproteomics.
37319314	1	23	gly	sites	357:361	arg1	the glycan			sites	the glycan					sites	Glycosylation is an essential protein modification occurring on the majority of extracellular human proteins, with mass spectrometry (MS) being an indispensable tool for its analysis, that not only determines glycan compositions, but also the position of the glycan at specific sites via glycoproteomics.
36499311	2	55	gly	O-glycosylation	588:602	arg2	extensive O-glycosylation sites			extensive O-glycosylation sites						sites	We report here its three-dimensional structure, showing the expected bimodular arrangement and also a unique long elongation at its N-terminus containing extensive O-glycosylation sites that form a peculiar arrangement with a protruding loop within the dimer.
35285541	4	5	gly	glycoprotein	617:628	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			Glycosylation influences the expected size of the glycoprotein, which leads to disproportionate MW estimation, in size-dependent methods.
37240090	3	21	gly	glycoprotein	611:622	arg1	a lectin-based glycoprotein microarray	a lectin-based glycoprotein microarray				Fterm		glycoprotein			The analytical methods used were a lectin-based glycoprotein microarray enabling high-throughput glycan analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) as a standard method for the identification of glycan structures.
34695439	2	35	gly	proteins	346:353	arg1	thrombospondin type I repeats	proteins			thrombospondin type I repeats	Fterm		proteins			B3GLCT mediates modification of proteins with thrombospondin type I repeats (TSR) that contain O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs.
34695439	2	46	gly	glycosylation	459:471	arg2	O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs			O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs						motifs	B3GLCT mediates modification of proteins with thrombospondin type I repeats (TSR) that contain O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs.
35247653	0	52	gly	hyposialylated	23:36	arg1	a novel hyposialylated erythropoietin	a novel hyposialylated erythropoietin				PUBTATOR		erythropoietin	2056		Characterizing a novel hyposialylated erythropoietin by intact glycoprotein and glycan analysis.
35247653	0	75	gly	glycoprotein	63:74	arg1	intact glycoprotein	intact glycoprotein				Fterm		glycoprotein			Characterizing a novel hyposialylated erythropoietin by intact glycoprotein and glycan analysis.
36388954	4	6	gly	protein	904:910	arg1	an increased glycan complexity	protein			an increased glycan complexity	Fterm		protein			Importantly, our model protein can take different routes of the cellular secretory pathway, resulting in an increased glycan complexity of the secreted protein.
35507105	0	65	gly	O-glycosylated	76:89	arg1	O-glycosylated insulin	O-glycosylated insulin				PUBTATOR		insulin	3630		Identifying signatures of proteolytic stability and monomeric propensity in O-glycosylated insulin using molecular simulation.
35776110	7	19	gly	glycoprotein	1385:1396	arg1	the model glycoprotein fetuin	the model glycoprotein fetuin				Fterm		glycoprotein			We evaluated this RB-GSM approach on N-glycan identification using MS/MS spectra acquired from glycan standards as well as those released from the model glycoprotein fetuin, immunoglobulin G, and human serum samples, which showed the RB-GSM is capable of distinguishing isomeric glycans.
36373229	6	34	gly	O-glycopeptide	1304:1317	arg2	O-glycopeptide identification			O-glycopeptide identification						O-glycopeptide	Using secreted protease of C1 esterase inhibitor (StcE) from enterohemorrhagic Escherichia coli and O-endoprotease OgpA from Akkermansia mucinophila, we explore numerous settings that effect O-glycopeptide identification and show how non-specific and semi-tryptic searches of O-glycopeptide data can produce candidate cleavage motifs.
36373229	6	74	gly	O-glycopeptide	1389:1402	arg2	O-glycopeptide data			O-glycopeptide data						O-glycopeptide	Using secreted protease of C1 esterase inhibitor (StcE) from enterohemorrhagic Escherichia coli and O-endoprotease OgpA from Akkermansia mucinophila, we explore numerous settings that effect O-glycopeptide identification and show how non-specific and semi-tryptic searches of O-glycopeptide data can produce candidate cleavage motifs.
33176830	6	8	gly	N-glycosylation	1134:1148	arg2	non-canonical motifs	Cav3.2		motifs		PUBTATOR		Cav3.2	8912	motifs	Therefore, these newly identified asparagine residues within non-canonical motifs add to those previously reported in canonical sites and suggest that N-glycosylation of Cav3.2 may also occur at non-canonical motifs to control expression of the channel in the plasma membrane.
35219398	1	54	gly	glycosylated	271:282	arg1	several immunoglobulin (Ig)-like ectodomains			several immunoglobulin (Ig)-like ectodomains						ectodomains	Carcinoembryonic cellular adhesion molecules (CEACAMs) serve diverse roles in cell signaling, proliferation, and survival and are made up of one or several immunoglobulin (Ig)-like ectodomains glycosylated in vivo.
32636304	4	22	gly	sites	733:737	arg1	the residues			the residues	the residues		AminoAcid			residues at	In this study, we report one such lectin named horcolin (Hordeum vulgare lectin), seen to lack mitogenicity owing to the divergence in the residues at its carbohydrate-binding sites, which makes it a promising candidate for exploration as an anti-HIV agent.
37146275	1	10	gly	glycosylation	253:265	arg2	glycosylation sites			glycosylation sites						sites	Glycosylation of proteins regulates the life activities of organisms, while abnormalities of glycosylation sites and glycan structures occur in various serious diseases such as cancer.
37146275	1	44	gly	Glycosylation	160:172	arg1	proteins	proteins				Fterm		proteins			Glycosylation of proteins regulates the life activities of organisms, while abnormalities of glycosylation sites and glycan structures occur in various serious diseases such as cancer.
35279850	6	29	gly	fucosylated	1146:1156	arg1	a novel fucosylated N-pentasaccharide				a novel fucosylated N-pentasaccharide						Glycomics profiling in patients with known defects revealed novel features such as the N-tetrasaccharide in ALG2-CDG patients and a novel fucosylated N-pentasaccharide as specific glycomarker for ALG1-CDG.
35925863	11	24	gly	glycoproteins	1761:1773	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			Such serum N-glycomic changes described herein may indicate or correlate to the changes in serum glycoproteins upon COVID-19 infection.
36435009	0	31	gly	core-fucosylated	24:39	arg1	core-fucosylated glycan-preferred ENGase	core-fucosylated glycan-preferred ENGase				OGER		ENGase	Q8NFI3		A fluorogenic probe for core-fucosylated glycan-preferred ENGase.
32417172	10	11	gly	Ser121	1375:1380	arg1	The O-GalNAc glycosylation at Ser121			Ser121	The O-GalNAc glycosylation at Ser121			tumor suppressor p53	P04637	Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
32417172	10	11	gly	Ser121	1375:1380	arg1	The O-GalNAc glycosylation at Ser121			Ser121	The O-GalNAc glycosylation at Ser121			tumor suppressor p53	P04637	Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
32417172	10	11	gly	Ser121	1375:1380	arg1	The O-GalNAc glycosylation at Ser121			Ser121	The O-GalNAc glycosylation at Ser121			tumor suppressor p53	P04637	Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
32417172	10	46	gly	glycosylation	1358:1370	arg2	The O-GalNAc glycosylation at Ser121			Ser121				tumor suppressor p53	P04637	Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
35995210	5	23	gly	N-glycosylation	767:781	arg2	up to eight putative N-glycosylation sites			up to eight putative N-glycosylation sites						sites	Our MALDI-TOF mass spectrometry data revealed that up to eight putative N-glycosylation sites were glycosylated.
35995210	5	84	gly	glycosylated	794:805	arg1	up to eight putative N-glycosylation sites			up to eight putative N-glycosylation sites						sites	Our MALDI-TOF mass spectrometry data revealed that up to eight putative N-glycosylation sites were glycosylated.
36031042	2	85	gly	glycoprotein	365:376	arg1	glycoprotein C3	glycoprotein C3				Fterm		glycoprotein			The most abundant complement component, glycoprotein C3, contains two N-glycosylation sites occupied exclusively by this type of glycans.
36031042	2	101	gly	N-glycosylation	395:409	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	The most abundant complement component, glycoprotein C3, contains two N-glycosylation sites occupied exclusively by this type of glycans.
36809652	7	46	gly	apo	1401:1403	arg1	intact O-glycan structures	apo(a			intact O-glycan structures	OGER		apo(a	P08519		We also demonstrated that the protein levels of galectin-1, NRP-1, VEGFR2, and downstream proteins in MAPK signaling were reduced in HUVECs in the presence of apo(a) with intact O-glycan structures compared to that of de-O-glycosylated apo(a).
36809652	7	10	gly	de-O-glycosylated	1460:1476	arg1	de-O-glycosylated apo	apo(a				OGER		apo(a	P08519		We also demonstrated that the protein levels of galectin-1, NRP-1, VEGFR2, and downstream proteins in MAPK signaling were reduced in HUVECs in the presence of apo(a) with intact O-glycan structures compared to that of de-O-glycosylated apo(a).
36435009	1	17	gly	core-fucosylated	286:301	arg1	core-fucosylated N-glycans				core-fucosylated N-glycans						A fluorescence-quenching-based assay system to determine the hydrolytic activity of endo-β-N-acetylglucosaminidases (ENGases), which act on the innermost N-acetylglucosamine (GlcNAc) residue of the chitobiose segment of core-fucosylated N-glycans, was constructed using a dual-labeled fluorescent probe with a hexasaccharide structure.
34691043	3	6	gly	glycosylation	427:439	arg2	predicted N-linked glycosylation sites			predicted N-linked glycosylation sites						sites	We previously showed that modulating predicted N-linked glycosylation sites on H3 A/Hong Kong/1/1968 HA identified a conserved epitope at the HA interface.
33085102	6	54	gly	O-glycosylated	907:920	arg1	42 O-glycosylated and 56 N-glycosylated proteins	42 O-glycosylated and 56 N-glycosylated proteins				Fterm		proteins			Analysis using LC/MS-QTOF identified 42 O-glycosylated and 56 N-glycosylated proteins, respectively.
33085102	6	65	gly	N-glycosylated	929:942	arg1	42 O-glycosylated and 56 N-glycosylated proteins	42 O-glycosylated and 56 N-glycosylated proteins				Fterm		proteins			Analysis using LC/MS-QTOF identified 42 O-glycosylated and 56 N-glycosylated proteins, respectively.
36324280	5	47	gly	glycosylation	1093:1105	arg2	the specific glycosylation site			the specific glycosylation site						site	The N-glycome analysis demonstrated that a degree of Hp deglycosylation differs depending on the conditions used for N-glycan release and on the specific glycosylation site, with Asn 241 being the most resistant to deglycosylation under tested conditions.
35887202	8	22	gly	O-glycopeptide	1168:1181	arg2	modeled MY.1E12 Fv and MUC1 O-glycopeptide			modeled MY.1E12 Fv and MUC1 O-glycopeptide						O-glycopeptide	Docking simulations using modeled MY.1E12 Fv and MUC1 O-glycopeptide, suggest that VH mainly contributes to the recognition of the MUC1 peptide portion while VL mainly binds to the O-glycan part.
36797772	2	22	gly	glycoprotein	418:429	arg1	a glycoprotein family	a glycoprotein family				Fterm		glycoprotein			FASCICLIN-LIKE ARABINOGALACTAN PROTEINs (FLAs), which impact plant growth and development, are an excellent example of a glycoprotein family with a complex multidomain structure.
36435935	0	2	gly	oligosaccharyltransferase	74:98	arg1	glycan recognition	oligosaccharyltransferase			glycan recognition	Fterm		oligosaccharyltransferase	281499		Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase.
35995381	10	31	gly	thyroglobulin	1529:1541	arg1	N-glycans	thyroglobulin			N-glycans	PUBTATOR		thyroglobulin	7038		BIOLOGICAL SIGNIFICANCE: N-glycans of human thyroglobulin modulate thyroid hormone synthesis both in vivo and in vitro.
36631484	9	4	gly	glycoproteins	2351:2363	arg1	model glycoproteins	model glycoproteins				Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
36631484	9	38	gly	glycosylation	2102:2114	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
36631484	9	83	gly	glycans	2334:2340	arg1	model glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
36631484	9	85	gly	glycoproteins	2070:2082	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
33486096	3	10	gly	proteins	474:481	arg1	O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
33486096	3	10	gly	proteins	474:481	arg1	The O-linked β-N-acetylglucosamine post-translational modification	proteins			The O-linked β-N-acetylglucosamine post-translational modification	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
33486096	3	10	gly	proteins	474:481	arg1	a glucose-derived metabolic signal	proteins			a glucose-derived metabolic signal	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
33486096	3	40	gly	modification	447:458	arg3	proteins AND The O-linked β-N-acetylglucosamine post-translational modification	proteins			The O-linked β-N-acetylglucosamine post-translational modification	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
34960798	10	32	gly	glycosylation	1731:1743	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	The number of potential N-linked glycosylation sites (PNGS) ranged from 3 to 6 in respective sequences and were located in different positions.
34985300	4	46	gly	glycopeptide	622:633	arg2	both HILIC and FAIMS based glycopeptide enrichment strategies			both HILIC and FAIMS based glycopeptide enrichment strategies						glycopeptide	We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
34985300	4	62	gly	glycosylation	703:715	arg2	257 putative glycosylation sites			257 putative glycosylation sites						sites	We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
34985300	4	81	gly	glycoproteins	730:742	arg1	145 glycoproteins	145 glycoproteins				Fterm		glycoproteins			We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
35487177	2	3	gly	glycosylation	411:423	arg1	2-azido-6-tert-butyldiphenylsilyl-3,4-di-O-chloroacetyl-2-deoxy-galctopyranosyl imidate and glucose moiety				2-azido-6-tert-butyldiphenylsilyl-3,4-di-O-chloroacetyl-2-deoxy-galctopyranosyl imidate and glucose moiety						The construction of Tn antigen moiety containing α-N-acetylgalactosamine residue was achieved by α-selective glycosylation of 2-azido-6-tert-butyldiphenylsilyl-3,4-di-O-chloroacetyl-2-deoxy-galctopyranosyl imidate and glucose moiety, which acts as a hydrophilic spacer when immobilized on a gold-coated sensor chip.
36159782	6	28	gly	non-glycosylated	1104:1119	arg1	the non-glycosylated IgG1	the non-glycosylated IgG1				OGER		IgG1	P01857		The LALA-PG mutated antibody showed no binding to the FcγIIa receptor (excluding potential non-specific binding effects) while the non-glycosylated IgG1 showed a strongly reduced, but still minor binding.
35380435	1	48	gly	glycosylated	213:224	arg1	the heavily glycosylated spike protein	the heavily glycosylated spike protein				PUBTATOR		spike protein	43740568		SARS-CoV-2 cellular infection is mediated by the heavily glycosylated spike protein.
35311852	8	101	gly	branch-fucosylated	1138:1155	arg1	branch-fucosylated N-glycan isomers				branch-fucosylated N-glycan isomers						To achieve better resolutions on permethylated glycans, different LC conditions were optimized using glycan standards, including core- and branch-fucosylated N-glycan isomers and sialic acid linked isomers, which were both successfully separated.
36740532	9	30	gly	O-glycosylated	1311:1324	arg1	The GPIbα mucin domain			The GPIbα mucin domain						domain	The GPIbα mucin domain is densely O-glycosylated.
36135182	2	34	gly	N-glycosylation	227:241	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo.
36135182	2	28	gly	has	219:221	arg1	N185			N185 and N398				Recombinant DSPAα2	112321404	N185 and N398	However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo.
35421698	3	2	gly	N-glycopeptides	511:525	arg2	intact N-glycopeptides			intact N-glycopeptides						N-glycopeptides	Here, we present a comprehensive structure-specific identification of intact N-glycopeptides of Arabidopsis with both HILIC and RAX enrichment, as well as GPSeeker and pGlyco database search.
33453988	2	39	gly	glycoprotein	415:426	arg1	glycoprotein clustering	glycoprotein clustering				Fterm		glycoprotein			Modification of cell surface receptors with branched N-glycans coordinates cell growth and differentiation by controlling glycoprotein clustering, signaling, and endocytosis.
32709309	8	30	gly	glycoprotein	1569:1580	arg1	several glycoprotein samples	several glycoprotein samples				Fterm		glycoprotein			This method was successfully used to analyze N-linked glycans released from several glycoprotein samples.
35687881	8	49	gly	sialylation	1397:1407	arg1	the N-glycans				the N-glycans						We also found that sialylation of the N-glycans significantly suppressed the immune response.
32417172	2	84	gly	have	318:321	arg1	nuclear or cytoplasmic proteins AND O-GalNAc glycosylation	nuclear or cytoplasmic proteins			O-GalNAc glycosylation	Fterm		proteins			Recently several evidences suggest that nuclear or cytoplasmic proteins might also have O-GalNAc glycosylation.
36959936	10	54	gly	glycoprotein	2028:2039	arg1	native glycoprotein structure	native glycoprotein structure				Fterm		glycoprotein			Nonetheless, animals immunized with the mammalian cell-culture-derived protein were better protected in this challenge model suggesting that more faithfully reproducing the native glycoprotein structure and associated glycosylation of the antigen may be desirable.
36925257	6	51	gly	F1-ESP-3	1088:1095	arg1	the monosaccharide composition	ESP			the monosaccharide composition	OGER		ESP	Q6UW49		Compared with the purified component L-ESP-3, the monosaccharide composition of F1-ESP-3 contains more glucuronic acid, the molecular weight reduced from >600 kDa (L-ESP-3) to 28.30 kDa (F1-ESP-3) and 33.58 kDa (F2-ESP-3), F1-ESP-3 has higher solubility and lower apparent viscosity.
37040463	11	20	gly	glycoproteins	1798:1810	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We anticipate that GlycoCAP will facilitate the development and discovery of glycan-based drugs by granting access to a wider variety of possible noncanonical glycan structures and also provide an approach for functionalizing glycoproteins by click chemistry conjugation.
35943155	16	106	gly	oligosaccharides	2700:2715	arg1	α-l-fucosyl residues			α-l-fucosyl residues	α-l-fucosyl residues		Site			residues	Microbial α-l-fucosidases are exoglycosidases that remove α-l-fucosyl residues from free oligosaccharides and glycoconjugates and can be also used in transglycosylation reactions to synthesize oligosaccharides.
35364496	5	26	gly	glycosylated	807:818	arg1	Glycinin	Glycinin				Fterm		Glycinin			Glycinin glycosylated with both polysaccharides exhibited enhanced emulsifying activity and stability.
35397991	5	59	gly	N-glycoproteins	889:903	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			N-glycan analysis of N-glycoproteins in the MSG demonstrated that BmGNTI identified and characterized in this study consistently contributed to GlcNAc extension of N-glycans, which led to the accumulation of GlcNAc-extended N-glycans as predominant structures throughout the MSG development.
36448941	8	68	gly	CMP	1306:1308	arg1	monosaccharide composition	CMP			monosaccharide composition	OGER		CMP	P21941		The purity, monosaccharide composition, molecular weight, and structural characteristics of CMP were different, but with similar infrared absorption spectra.
36031042	5	69	gly	glycopeptide	841:852	arg2	subsequent LC-MS glycopeptide analysis			subsequent LC-MS glycopeptide analysis						glycopeptide	For this purpose, we developed a novel high-throughput workflow for human C3 concanavalin A lectin affinity enrichment and subsequent LC-MS glycopeptide analysis which enables protein-specific N-glycosylation profiling.
33244686	3	49	gly	glycopeptides	745:757	arg2	glycopeptides			glycopeptides						glycopeptides	Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abundance non-glycosylated peptides, which can be further improved by modifying the mobile phases with ion-pairing agents (IP-HILIC).
33244686	3	68	gly	glycopeptide	626:637	arg2	better glycopeptide identification			better glycopeptide identification						glycopeptide	Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abundance non-glycosylated peptides, which can be further improved by modifying the mobile phases with ion-pairing agents (IP-HILIC).
33244686	3	97	gly	non-glycosylated	779:794	arg1	high-abundance non-glycosylated peptides			high-abundance non-glycosylated peptides						peptides	Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abundance non-glycosylated peptides, which can be further improved by modifying the mobile phases with ion-pairing agents (IP-HILIC).
37341462	3	71	gly	O-glycopeptides	580:594	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	However, the pattern of Y ions in O-glycopeptides has not been well studied.
37328468	0	53	gly	glycoproteins	59:71	arg1	divergent Henipavirus fusion glycoproteins	divergent Henipavirus fusion glycoproteins				Fterm		glycoproteins			Structure and antigenicity of divergent Henipavirus fusion glycoproteins.
35405095	3	31	gly	O-glycosylated	486:499	arg1	densely O-glycosylated domains			densely O-glycosylated domains						domains	In particular, the ability to degrade host mucins, a class of proteins carrying densely O-glycosylated domains, provides a competitive advantage in the sustained colonization of niche mucosal environments.
36192063	4	21	gly	N-glycopeptide	803:816	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	With lung cancer as a model system, differential sialylation in cancer tissues relative to adjacent non-tumor tissues was characterized at the intact N-glycopeptide level with N-glycosite information.
36192063	4	68	gly	N-glycosite	829:839	arg2	N-glycosite			N-glycosite						N-glycosite	With lung cancer as a model system, differential sialylation in cancer tissues relative to adjacent non-tumor tissues was characterized at the intact N-glycopeptide level with N-glycosite information.
37037133	4	19	gly	N-glycoproteins	674:688	arg1	1,360, 457, 2,617, and 986 site-specific N-glycans	N-glycoproteins			1,360, 457, 2,617, and 986 site-specific N-glycans	Fterm		N-glycoproteins			We identified 1,360, 457, 2,617, and 986 site-specific N-glycans from 296, 77, 214, and 196 N-glycoproteins in donkey colostrum (DC), donkey mature milk (DM), human colostrum (HC), and human mature milk (HM), respectively.
35713525	5	36	gly	attached	606:613	arg1	Sur1 AND N-glycans	Sur1			N-glycans	PUBTATOR		Sur1	856050		In this study, we elucidated the roles played by N-glycans attached to Sur1 and Csh1, and dissected the mechanisms underlying substrate recognition by these 2 enzymes.
35713525	5	36	gly	attached	606:613	arg1	Csh1 AND N-glycans	Csh1			N-glycans	PUBTATOR		Csh1	852458		In this study, we elucidated the roles played by N-glycans attached to Sur1 and Csh1, and dissected the mechanisms underlying substrate recognition by these 2 enzymes.
36797772	3	42	gly	glycans	705:711	arg1	the FAS1 domains			the FAS1 domains	the FAS1 domains		Site			domains	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	80	gly	FAS1	514:517	arg1	N-linked glycans	FAS1			N-linked glycans	OGER		FAS1	P25445		FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36189205	4	2	gly	FcγRIIIa	813:820	arg1	different N-glycan structures	FcγRIIIa			different N-glycan structures	PUBTATOR		FcγRIIIa	2214		In this study, we employed comprehensive genetic engineering of the N-glycosylation capacities in mammalian cell lines to express IgG1 and FcγRIIIa with different N-glycan structures to more generally explore the role of N-glycosylation in IgG1:FcγRIIIa binding interactions.
36189205	4	9	gly	IgG1	804:807	arg1	different N-glycan structures	IgG1			different N-glycan structures	OGER		IgG1	P01857		In this study, we employed comprehensive genetic engineering of the N-glycosylation capacities in mammalian cell lines to express IgG1 and FcγRIIIa with different N-glycan structures to more generally explore the role of N-glycosylation in IgG1:FcγRIIIa binding interactions.
33085102	5	49	gly	glycosylated	791:802	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			The glycoproteome profiling demonstrates that glycosylated proteins are higher in caprine milk compared to other samples.
33727825	1	16	gly	glycoprotein	159:170	arg1	a highly conserved glycoprotein	a highly conserved glycoprotein				Fterm		glycoprotein			BACKGROUND Ribophorin II (RPN2) is a highly conserved glycoprotein involved in the N-linked glycosylation of multiple proteins.
33727825	1	16	gly	glycoprotein	159:170	arg1	BACKGROUND Ribophorin II	BACKGROUND Ribophorin II				PUBTATOR		Ribophorin II	6185		BACKGROUND Ribophorin II (RPN2) is a highly conserved glycoprotein involved in the N-linked glycosylation of multiple proteins.
33727825	1	25	gly	glycosylation	197:209	arg1	multiple proteins	multiple proteins				Fterm		proteins			BACKGROUND Ribophorin II (RPN2) is a highly conserved glycoprotein involved in the N-linked glycosylation of multiple proteins.
36534501	6	11	gly	lumican	1043:1049	arg1	glycan chains	lumican			glycan chains	PUBTATOR		lumican	4060		The impact of the number of glycan chains, structures, and dynamics of lumican on the interaction with MMP-14 was assessed by molecular dynamics simulations.
36241003	1	34	gly	glycosylated	116:127	arg1	highly glycosylated proteins	highly glycosylated proteins				Fterm		proteins			Mucins are highly glycosylated proteins that make up the mucus covering internal and external surfaces of fish.
35577189	7	27	gly	cAMPF	1280:1284	arg1	a functional edible polysaccharide film	cAMPF			a functional edible polysaccharide film	Cterm		cAMPF	P49913		All these data suggested the potential value of cAMPF as a functional edible polysaccharide film applied in food industries.
35388686	2	38	gly	glycosites	496:505	arg1	glycan compositions			glycosites	glycan compositions					glycosites	Here, we have adapted an HCD-triggered-ultraviolet photodissociation (UVPD) approach for the simultaneous localization of glycosites and full characterization of both glycan compositions and intersaccharide linkages, the latter provided by extensive cross-ring cleavages enabled by UVPD.
35388686	2	38	gly	glycosites	496:505	arg1	intersaccharide linkages			glycosites	intersaccharide linkages					glycosites	Here, we have adapted an HCD-triggered-ultraviolet photodissociation (UVPD) approach for the simultaneous localization of glycosites and full characterization of both glycan compositions and intersaccharide linkages, the latter provided by extensive cross-ring cleavages enabled by UVPD.
37382290	3	44	gly	glycoproteins	534:546	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The ability to distinguish between these complex glycan structures poses a significant challenge, hindering our ability to accurately measure and understand the role of glycoproteins in biological systems.
33176830	0	60	gly	N-glycosylation	53:67	arg2	potential non-canonical N-glycosylation sites			potential non-canonical N-glycosylation sites						sites	Functional identification of potential non-canonical N-glycosylation sites within Cav3.2 T-type calcium channels.
37331167	1	41	gly	glycoproteins	318:330	arg1	insect glycoproteins	glycoproteins			core pentasaccharide	Fterm		glycoproteins			Core α-1,3 mannose is structurally near the core xylose and core fucose on core pentasaccharide from plant and insect glycoproteins.
36252443	7	91	gly	glycoproteins	1171:1183	arg1	bovine milk glycoproteins	bovine milk glycoproteins				Fterm		glycoproteins			infantis ATCC 15697 (B. infantis) was characterized as having the ability to release N-glycans from bovine milk glycoproteins efficiently, without the denaturation.
35091091	6	16	gly	N-glycopeptide	1566:1579	arg2	N-glycopeptide spectra			N-glycopeptide spectra						N-glycopeptide	The new method has been incorporated into the PTM-Shepherd modification analysis tool to work directly with the MSFragger glyco search in the FragPipe graphical user interface, providing a complete computational pipeline for annotation of N-glycopeptide spectra with false discovery rate control of both peptide and glycan components that is both sensitive and robust against false identifications.
35091091	6	25	gly	peptide	1631:1637	arg1	both peptide and glycan components				both peptide and glycan components						The new method has been incorporated into the PTM-Shepherd modification analysis tool to work directly with the MSFragger glyco search in the FragPipe graphical user interface, providing a complete computational pipeline for annotation of N-glycopeptide spectra with false discovery rate control of both peptide and glycan components that is both sensitive and robust against false identifications.
33184803	7	69	gly	glycoprotein	1201:1212	arg1	plasma glycoprotein profile	plasma glycoprotein profile				Fterm		glycoprotein			Mass spectrometry revealed a dramatic alternation in plasma glycoprotein profile in patients with iTTP compared with the healthy controls.
34012659	2	35	gly	N-glycoprotein	310:323	arg1	an N-glycoprotein	an N-glycoprotein				Fterm		N-glycoprotein			Bone marrow stromal cell antigen 2 (BST-2), an N-glycoprotein, has been characterized as an oncogenic factor in several types of cancer.
35343965	7	67	gly	glycoprotein	1345:1356	arg1	the target glycoprotein	the target glycoprotein				Fterm		glycoprotein			The functional importance of glycosylation has been elucidated in several bacteria through mutation of specific genes thought to be involved in the glycosylation process and the study of its impact on the expression of the target glycoprotein and the modifying glycan.
33119615	3	63	gly	glycosylation	556:568	arg1	residue N81			residue N81				RAGE	177	residue N81	Our previous in silico study indicated a higher binding affinity for mutated G82S RAGE, which could be caused due to changes in N linked glycosylation at residue N81.
37366623	3	85	gly	glycosylation	752:764	arg2	the glycosylation site			the glycosylation site						site	We also demonstrated that the presence of human γ-globulins in mice and immunity to HSV-1 induced by viral infection in mice significantly reduced replication in their eyes of a mutant virus lacking the glycosylation site but had little effect on the replication of its repaired virus.
35320529	3	74	gly	modified	581:588	arg3	some critical lymphocyte receptors AND glycan structures	some critical lymphocyte receptors			glycan structures	Fterm		receptors			Several galectins have a varying degree of affinity for the N-acetyllactosamine (LacNAc) disaccharide, and some critical lymphocyte receptors can be modified by glycan structures carrying this motif.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	surface lipoproteins	surface lipoproteins				Fterm		lipoproteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	abundant T9SS cargo proteins	proteins				Fterm		proteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	outer membrane β-barrel proteins	proteins				Fterm		proteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	outer membrane β-barrel proteins	proteins				Fterm		proteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
33199824	9	40	gly	modification	1104:1115	arg1	SMAD4 Thr63	SMAD4			modification	PUBTATOR		SMAD4	4089		In addition, O-GlcNAc modification on SMAD4 Thr63 was responsible for stabilization.
33085102	8	41	gly	glycoproteins	1124:1136	arg1	Only glycoproteins	Only glycoproteins				Fterm		glycoproteins			Only glycoproteins from human milk have shown positive matching to important human biological pathways, such as vesicle-mediated transport, immune system and hemostasis pathways.
34154738	1	101	gly	fucosylation	105:116	arg1	an Fc N-linked glycan				an Fc N-linked glycan						Core fucosylation of an Fc N-linked glycan affects antibody effector functions, as the absence of fucose increases the antibody dependent cell cytotoxicity (ADCC) response with increased binding to the Fcγ receptors.
36535347	5	35	gly	SRP	772:774	arg1	The carbohydrate content	SRP			The carbohydrate content	OGER		SRP	Q96RP3		The carbohydrate content of SRP was 85.09 %, with a relatively high content of uronic acids (11.27 %).
37162352	4	11	gly	O-glycosylation	592:606	arg2	325 O-glycosylation sites			325 O-glycosylation sites						sites	In this study, we characterized the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins.
37162352	4	26	gly	glycoproteins	625:637	arg1	226 glycoproteins	226 glycoproteins				Fterm		glycoproteins			In this study, we characterized the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins.
36660462	5	10	gly	glycopeptides	868:880	arg2	789 glycopeptides			789 glycopeptides						glycopeptides	A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
36660462	5	13	gly	glycosylated	938:949	arg1	204 glycosylated sites			204 glycosylated sites						sites	A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
36660462	5	31	gly	glycoproteins	964:976	arg1	159 glycoproteins	159 glycoproteins				Fterm		glycoproteins			A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
37162352	6	62	gly	glycoproteins	803:815	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			Bioinformatic localization of the glycoproteins predicted 68 inner membrane proteins, 60 periplasmic proteins, 26 outer membrane proteins, 57 lipoproteins, and 9 proteins secreted by the T9SS.
36972173	3	50	gly	glycoproteins	558:570	arg1	spike glycoproteins	spike glycoproteins				Fterm		glycoproteins			This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins.
37366623	9	8	gly	glycoprotein	1738:1749	arg1	the envelope glycoprotein B	the envelope glycoprotein B				Cterm		the envelope glycoprotein B (gB			Here, we show that an N-glycan shield on the specific site of the envelope glycoprotein B (gB) of HSV-1 mediates evasion from pooled γ-globulins derived from human blood both in cell cultures and mice.
37037133	3	36	gly	glycoproteins	520:532	arg1	donkey and human MFGM glycoproteins	donkey and human MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		This study aimed to map the most comprehensive site-specific N-glycosylation fingerprinting of donkey and human MFGM glycoproteins using a site-specific glycoproteomics strategy.
36136114	0	66	gly	glycosylated	48:59	arg1	engineered heavily glycosylated fusion proteins	engineered heavily glycosylated fusion proteins				Fterm		proteins			O-Glycoproteomic analysis of engineered heavily glycosylated fusion proteins using nanoHILIC-MS.
36493594	10	26	gly	glycoproteins	1947:1959	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This study is the first comparative analysis for the identification and relative and absolute quantification of N-glycans in glycoproteins with PF-ProA and PA-ProA using UPLC and LC-MS/MS.
37186866	5	3	gly	domains	1216:1222	arg1	O-mannosylation			domains	O-mannosylation					domains	Thus, our study identifies the third protein-specific O-mannosylation pathway in mammals and demonstrates that O-mannosylation of IPT domains serves critical functions during epithelial morphogenesis.
37186866	5	31	gly	O-mannosylation	1193:1207	arg1	IPT domains			IPT domains						domains	Thus, our study identifies the third protein-specific O-mannosylation pathway in mammals and demonstrates that O-mannosylation of IPT domains serves critical functions during epithelial morphogenesis.
36435009	2	11	gly	fucosylated	539:549	arg1	core fucosylated N-glycans				core fucosylated N-glycans						The fluorogenic probe was evaluated using a variety of ENGases, including Endo-M W251N mutant, Endo-F3, and Endo-S, which recognize core fucosylated N-glycans.
34726173	1	54	gly	serine-aspartate	230:245	arg1	SDR			serine	SDR					serine	The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA.
34726173	1	54	gly	serine-aspartate	230:245	arg1	serine-aspartate repeat			serine	serine-aspartate repeat					serine	The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA.
34726173	1	80	gly	containing	219:228	arg1	staphylococcal adhesive proteins AND serine-aspartate repeat (SDR) domains	staphylococcal adhesive proteins			serine-aspartate repeat (SDR) domains	Fterm		proteins			The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA.
35921896	6	6	gly	asparagine-linked	934:950	arg1	the CD16a asparagine-linked (N)-glycans			asparagine	the CD16a asparagine-linked (N)-glycans					asparagine	Another relevant variable shown to affect affinity is composition of the CD16a asparagine-linked (N)-glycans.
33583770	10	28	gly	N-glycosylation	2006:2020	arg1	galectin-1	galectin-1				PUBTATOR		galectin-1	16852		Importantly, similar changes in N-glycosylation and the upregulation of galectin-1 during postnatal skeletal muscle development were observed in mice.
36670130	7	19	gly	N-glycosylation	1162:1176	arg2	all N-glycosylation sites			all N-glycosylation sites						sites	Intriguingly, the removal of all N-glycosylation sites on MP84 increased adhesion to host cells and enhanced the induction of cytokine secretion from BMDCs compared with that on MP84 carrying WT N-glycans.
36812846	5	46	gly	α-mannosylation	767:781	arg1	the C-3 and C-6 positions			the C-3 and C-6 positions						positions	Sequential regioselective α-mannosylation at the C-3 and C-6 positions of 2,3,4,6-unprotected galactose residues in galactosylchitobiose derivatives was demonstrated.
36812846	5	61	gly	positions	802:810	arg1	Sequential regioselective α-mannosylation			positions	Sequential regioselective α-mannosylation					positions	Sequential regioselective α-mannosylation at the C-3 and C-6 positions of 2,3,4,6-unprotected galactose residues in galactosylchitobiose derivatives was demonstrated.
36838558	0	32	gly	Protein	107:113	arg1	the Glycan Structure	Protein			the Glycan Structure	Fterm		Protein			Investigation of the Protective Effect for GcMAF by a Glycosidase Inhibitor and the Glycan Structure of Gc Protein.
35495330	5	1	gly	residues	1250:1257	arg1	glutamic acid			glutamic acid						acid	We were utilizing a triazole bioisostere linkage to form the respective mannosylated-derivative on the HA peptide sequence of residues glutamine, glycine, and glutamic acid.
36925243	0	2	gly	β-cyclodextrin	81:94	arg1	isomalto/malto-polysaccharides	β-cyclodextrin			isomalto/malto-polysaccharides	Fterm		β-cyclodextrin			Cost-effective and controllable synthesis of isomalto/malto-polysaccharides from β-cyclodextrin by combined action of cyclodextrinase and 4,6-α-glucanotransferase GtfB.
36380917	5	45	gly	peptides	1255:1262	arg1	the therapeutic properties				the therapeutic properties						Our results indicated that the selection of an appropriate position (to avoid hindering the peptide-receptor binding) is crucial for N-glycan modification and its sialylation to improve the therapeutic properties of the modified peptides.
36990248	1	28	gly	O-glycosylation	123:137	arg1	secreted and membrane-bound proteins	secreted and membrane-bound proteins				Fterm		proteins			O-glycosylation of secreted and membrane-bound proteins is an important post-translational modification that affects recognition of cell surface receptors, protein folding, and stability.
37219590	4	7	gly	glycoprotein	750:761	arg1	glycoprotein models	glycoprotein models				Fterm		glycoprotein			Here, a broadening of the scope of the software to analyse and validate the overall conformation of N-glycans is presented, focusing on a newly compiled set of glycosidic linkage torsional preferences harvested from a curated set of glycoprotein models.
34102146	4	31	gly	glycopeptide	892:903	arg2	intact glycopeptide identification			intact glycopeptide identification						glycopeptide	Results identified haptoglobin as the protein associated with HexNAc4-Hex5-NeuAc2, thus directly linking glycan imaging with intact glycopeptide identification.
36914840	7	51	gly	glycoforms	1431:1440	arg1	the IgG3-G2 and S1 glycoforms	the IgG3-G2 and S1 glycoforms				OGER		IgG3	P01860		However, vancomycin use during pregnancy was associated with changes in IgG-Fc glycosylation in offspring serum, shown by the decreased relative abundance of the IgG1F-G1 and IgG3-G0 glycoforms, together with the increased relative abundance of the IgG3-G2 and S1 glycoforms.
36892535	4	24	gly	O-glycosylated	598:611	arg1	homogeneously O-glycosylated MUC1 peptides			homogeneously O-glycosylated MUC1 peptides						peptides	To better understand the role of MUC1 glycosylation in interaction and adhesion of cancer cells, we prepared a panel of homogeneously O-glycosylated MUC1 peptides using a quantitative chemoenzymatic approach.
34274643	1	62	gly	glycoprotein	342:353	arg1	an intact glycoprotein	an intact glycoprotein				Fterm		glycoprotein			Separations based on combinations of 2.1 mm I.D. high-performance affinity microcolumns and capillary electrophoresis were developed and used to characterize the glycoforms of an intact glycoprotein.
34274643	1	79	gly	glycoforms	318:327	arg1	an intact glycoprotein	an intact glycoprotein				Fterm		glycoprotein			Separations based on combinations of 2.1 mm I.D. high-performance affinity microcolumns and capillary electrophoresis were developed and used to characterize the glycoforms of an intact glycoprotein.
35123071	6	2	gly	glycoproteins	1039:1051	arg1	41 glycoproteins	41 glycoproteins				Fterm		glycoproteins			A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35123071	6	16	gly	glycoproteins	1133:1145	arg1	these glycoproteins	these glycoproteins				Fterm		glycoproteins			A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35123071	6	31	gly	glycopeptides	1061:1073	arg2	43 glycopeptides			43 glycopeptides						glycopeptides	A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35941485	1	15	gly	glycosylated	175:186	arg1	Proteoglycans	proteins			Proteoglycans	Fterm		proteins			Proteoglycans are heavily glycosylated proteins, covalently linked to one or more glycosaminoglycan (GAG) chains, abundantly expressed in the extracellular matrix (ECM).
36055406	0	65	gly	glycosylation	21:33	arg1	GPI-anchored proteins	GPI-anchored proteins				Fterm		proteins			ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
36740532	0	83	gly	glycoprotein	35:46	arg1	platelet glycoprotein	platelet glycoprotein				Fterm		glycoprotein			Comprehensive analysis of platelet glycoprotein Ibα ectodomain glycosylation.
35395480	5	13	gly	pectin	602:607	arg1	monosaccharide compositions	pectin			monosaccharide compositions	Fterm		pectin			Structural characteristics of pectin, including the molecular weight, degree of methoxylation and acetylation, and monosaccharide compositions were measured.
36870092	9	54	gly	N-glycopeptides	1299:1313	arg2	1717 quantified intact N-glycopeptides			1717 quantified intact N-glycopeptides						N-glycopeptides	Among 1717 quantified intact N-glycopeptides, 104 DEGPs were determined (fold change ≥ 1.5 and p value < 0.05).
36370046	7	1	gly	glycosylation	994:1006	arg1	the spike protein receptor-binding domain			the spike protein receptor-binding domain						domain	Herein, we used designed combinations of glycoengineering enzymes to simplify and control the glycosylation profile of the spike protein receptor-binding domain (RBD).
35293727	3	46	gly	glycosylation	478:490	arg2	multiple glycosylation sites			multiple glycosylation sites						sites	O-Antigen polysaccharides are typically polydisperse, and carrier proteins can have multiple glycosylation sites.
35780667	6	11	gly	composition	916:926	arg1	neutral sugars			position	neutral sugars					position	The different structure of the two polysaccharide fractions was also confirmed by the composition in neutral sugars and the degrees of methylation and acetylation.
36244450	5	52	gly	glycosylation	823:835	arg1	receptor glycan-binding properties				receptor glycan-binding properties						The construct was expressed in different glycosylation-mutant cell lines to study the influence of differential glycosylation on receptor glycan-binding properties.
36029899	4	73	gly	N-glycosylation	504:518	arg1	ADA2	ADA2				PUBTATOR		ADA2	51816		METHODS We investigated the roles of N-glycosylation in the activity, homodimerization, and secretion of ADA2 via site-directed mutagenesis and the application of N-glycosylation inhibitors.
36192063	2	36	gly	N-glycopeptides	447:461	arg2	intact N-glycopeptides level			intact N-glycopeptides level						N-glycopeptides	Mass spectrometry-based quantitative N-glycoproteomics has been widely adopted for quantitative analysis of aberrant sialylation, yet multiplexing method at intact N-glycopeptides level is still lacking.
32409323	1	49	gly	glycoproteins	214:226	arg1	high mannose N-linked glycoproteins				high mannose N-linked glycoproteins						OBJECTIVES FBXO6, a component of the ubiquitin E3 ligases, has been shown to bind high mannose N-linked glycoproteins and act as ubiquitin ligase subunits.
35285541	3	43	gly	heterogeneity	522:534	arg1	the attached carbohydrates				the attached carbohydrates						Determination of MW of heavily glycosylated proteins, such as Fc-fusion proteins, is seriously hampered by the physicochemical characteristics and heterogeneity of the attached carbohydrates.
35285541	3	37	gly	glycosylated	406:417	arg1	heavily glycosylated proteins	proteins				Fterm		proteins			Determination of MW of heavily glycosylated proteins, such as Fc-fusion proteins, is seriously hampered by the physicochemical characteristics and heterogeneity of the attached carbohydrates.
34611869	7	28	gly	glycosylated	1283:1294	arg1	the glycosylated asparagine side chains	the glycosylated asparagine side chains				OGER		chains			Therefore, in these structures we modified the Fc glycan regions by adjusting the glycosylated asparagine side chains and glycosidic bonds.
36089065	4	0	gly	glycoproteins	749:761	arg1	reporter N-linked glycoproteins	reporter N-linked glycoproteins				Fterm		glycoproteins			We tested this hypothesis via expression of reporter N-linked glycoproteins in N-acetylglucosaminyltransferase MGAT1-null HEK293 cells to produce immature Man5GlcNAc2 glycoforms (38 glycan sites total).
36089065	4	57	gly	glycoforms	854:863	arg1	immature Man5GlcNAc2 glycoforms				immature Man5GlcNAc2 glycoforms						We tested this hypothesis via expression of reporter N-linked glycoproteins in N-acetylglucosaminyltransferase MGAT1-null HEK293 cells to produce immature Man5GlcNAc2 glycoforms (38 glycan sites total).
35481895	0	13	gly	protein	94:100	arg1	N-	protein			N-	Fterm		protein			Characterizing the N- and O-linked glycans of the PGF-CTERM sorting domain-containing S-layer protein of Methanoculleus marisnigri.
35481895	0	13	gly	protein	94:100	arg1	O-linked glycans	protein			O-linked glycans	Fterm		protein			Characterizing the N- and O-linked glycans of the PGF-CTERM sorting domain-containing S-layer protein of Methanoculleus marisnigri.
36181511	0	61	gly	glycopeptide	35:46	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	Evaluation of absorbent cotton for glycopeptide enrichment.
36606688	4	57	gly	N-glycosylation	437:451	arg1	SOD3	SOD3				PUBTATOR		SOD3	6649		Although we reported previously that the N-glycosylation of SOD3 was essential for its secretion, the role played by the N-glycosylation of SOD3, as it relates to lung cancer, is poorly understood.
36606688	4	66	gly	N-glycosylation	517:531	arg1	SOD3	SOD3				PUBTATOR		SOD3	6649		Although we reported previously that the N-glycosylation of SOD3 was essential for its secretion, the role played by the N-glycosylation of SOD3, as it relates to lung cancer, is poorly understood.
36746580	4	17	gly	N-glycans	845:853	arg1	25 N-glycoproteins	N-glycoproteins			N-glycans	Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
36746580	4	37	gly	N-glycans	766:774	arg1	10 N-glycoproteins	N-glycoproteins			N-glycans	Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
36746580	4	68	gly	N-glycoproteins	782:796	arg1	10 N-glycoproteins	10 N-glycoproteins				Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
36746580	4	82	gly	N-glycoproteins	861:875	arg1	25 N-glycoproteins	25 N-glycoproteins				Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
32691273	14	87	gly	proteins	2314:2321	arg1	O-GlcNAc levels	proteins			O-GlcNAc levels	Fterm		proteins			In conclusion, our results suggest that OGT regulates insulin receptor signaling and unfolded protein response by modulating O-GlcNAc levels of total proteins, in response to insulin resistance.
37348475	0	8	gly	glycosylation	90:102	arg1	a classical arabinogalactan protein precursor	a classical arabinogalactan protein precursor				Fterm		precursor			Glycosylphosphatidylinositol-anchoring is required for the proper transport and extensive glycosylation of a classical arabinogalactan protein precursor in tobacco BY-2 cells.
36870654	0	69	gly	glycosylation	36:48	arg1	carbohydrate substrate binding				carbohydrate substrate binding						Insights into the effect of protein glycosylation on carbohydrate substrate binding.
35647713	4	41	gly	glycoproteins	770:782	arg1	viral glycoproteins	glycoproteins			N-linked glycans	Fterm		glycoproteins			We describe herein a one-step enzymatic conjugation approach for the covalent attachment of small molecule adjuvants to N-linked glycans of viral glycoproteins.
36972173	7	19	gly	glycosylation	880:892	arg2	glycosylation sites			glycosylation sites						sites	Conversely, glycosylation sites in the S2 domain are highly conserved and contain a low abundance of oligomannose-type glycans, suggesting a low glycan shield density.
37162352	12	64	gly	glycosylated	1526:1537	arg1	The glycosylated domains			The glycosylated domains						domains	The glycosylated domains were mainly localized to the periplasm.
34274643	0	75	gly	glycoprotein	111:122	arg1	alpha1-acid glycoprotein	alpha1-acid glycoprotein				Fterm		glycoprotein			Glycoprotein analysis using lectin microcolumns and capillary electrophoresis: Characterization of alpha1-acid glycoprotein by combined separation methods.
35104505	6	54	gly	glycoprotein	1212:1223	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			To clarify this mechanism, we measured the in vitro activity of purified GnT-VΔN toward various kinds of substrates (oligosaccharide, glycohexapeptide, and glycoprotein) using HPLC and a UDP-Glo assay.
35943155	12	10	gly	fucosylated	1996:2006	arg1	fucosylated glycans				fucosylated glycans						These results give insight into the great diversity of α-l-fucosidases from the infant gut microbiota, thus supporting the hypothesis that fucosylated glycans are crucial for shaping the newborn microbiota composition.
36892535	7	30	gly	glycosylated	1067:1078	arg1	the glycosylated MUC1 peptides			the glycosylated MUC1 peptides						peptides	To decipher the molecular mechanism for the observed adhesion we investigated conformation of the glycosylated MUC1 peptides by NMR.
36580234	8	23	gly	glycoproteins	1324:1336	arg1	target glycoproteins	target glycoproteins				Fterm		glycoproteins			The precision structural interpretation of site-specific glycans and subsequent intervention of target glycoproteins and related glycosyltransferases are of great value for the development of new diagnostic and therapeutic approaches for different diseases.
35887202	9	2	gly	anti-glycopeptide	1430:1446	arg2	anti-glycopeptide			anti-glycopeptide						anti-glycopeptide	The VH/VL-shared recognition mode of this antibody may be used as a template for the rational design and development of anti-glycopeptide antibodies.
35104505	3	14	gly	glycoprotein	567:578	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			However, it is still unclear how GnT-V selectively acts on glycoprotein substrates.
37093892	12	37	gly	glycosylation	1747:1759	arg2	the highly conserved glycosylation site N8			the highly conserved glycosylation site N8						site	We identified three major epitopic regions: the loops at the apex of the RBD, which likely mediate interactions between Env protomers to form Env trimers, a loop located in the vicinity of the heparan binding site, and a region proximal to the highly conserved glycosylation site N8.
36189205	3	22	gly	N-glycosylation	450:464	arg1	receptor interactions	receptor interactions				Fterm		receptor			Studies have suggested that also N-glycosylation of the FcγRIII affects receptor interactions with IgG, but detailed studies of the interaction of IgG1 and FcγRIIIa with distinct N-glycans have been hindered by the natural heterogeneity in N-glycosylation.
36189205	3	22	gly	N-glycosylation	450:464	arg1	the FcγRIII	the FcγRIII				PUBTATOR		FcγRIII	2214		Studies have suggested that also N-glycosylation of the FcγRIII affects receptor interactions with IgG, but detailed studies of the interaction of IgG1 and FcγRIIIa with distinct N-glycans have been hindered by the natural heterogeneity in N-glycosylation.
36385669	5	1	gly	glycosylation	874:886	arg2	a glycosylation site			a glycosylation site						site	Furthermore, based on sequence Ans-X-Ser/Thr, where X is any amino acid except proline, a glycosylation site was determined on asparagine 36.
37060669	8	44	gly	fucosylated	1185:1195	arg1	fucosylated human milk oligosaccharides				fucosylated human milk oligosaccharides						This work describes a novel fucosylated oligosaccharide and its preparation method, and the obtained trisaccharide may serve as a promising candidate for fucosylated human milk oligosaccharides.
37060669	8	45	gly	fucosylated	1059:1069	arg1	a novel fucosylated oligosaccharide				a novel fucosylated oligosaccharide						This work describes a novel fucosylated oligosaccharide and its preparation method, and the obtained trisaccharide may serve as a promising candidate for fucosylated human milk oligosaccharides.
36631484	6	19	gly	glycoprotein	1237:1248	arg1	glycoprotein engineering	glycoprotein engineering				Fterm		glycoprotein			Here we report a simplified approach to assist in monitoring glycosylation features during glycoprotein engineering, that employs flow cytometry using fluorescent microspheres chemically coupled to high-specificity glycan binding reagents.
35411940	2	50	gly	proteins	329:336	arg1	N-linked high-mannose glycans	proteins			N-linked high-mannose glycans	Fterm		proteins			Sickle cell disease and infection by Plasmodium falciparum cause oxidative stress that induces aggregates of cross-linked proteins with N-linked high-mannose glycans (HMGs).
35091091	4	74	gly	glycopeptides	784:796	arg1	the glycan composition			glycopeptides	the glycan composition					glycopeptides	Here, we have developed a new method for determining the glycan composition of N-linked glycopeptides fragmented by collisional or hybrid activation that uses multiple sources of information from the spectrum, including observed glycan B-type (oxonium) and Y-type ions and mass and precursor monoisotopic selection errors to discriminate between possible glycan candidates.
35349291	5	38	gly	N-glycopeptide	622:635	arg2	Our recently developed intact N-glycopeptide search engine GPSeeker			Our recently developed intact N-glycopeptide search engine GPSeeker						N-glycopeptide	Our recently developed intact N-glycopeptide search engine GPSeeker has made structure-specific characterization of N-glycosylation possible with structure-diagnostic fragment ions from selective fragmentation of N-glycan moieties.
36235191	6	58	gly	DDO	1465:1467	arg1	three polysaccharide fragments	DDO			three polysaccharide fragments	OGER		DDO	Q99489		The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO.
36235191	6	59	gly	fragments	1417:1425	arg1	the DDO	DDO			fragments	OGER		DDO	Q99489		The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO.
33542268	6	19	gly	glycosylation	1388:1400	arg2	the sole stabilizing N-linked glycosylation site			the sole stabilizing N-linked glycosylation site						site	To uncover potential inherent F3 instability not easily observed under typical culture conditions, we genetically eliminated the sole stabilizing N-linked glycosylation site (N249) from select clinically-identified F3 mutants.
33542268	6	19	gly	glycosylation	1388:1400	arg2	N249			N249				fibulin-3	2202	N249	To uncover potential inherent F3 instability not easily observed under typical culture conditions, we genetically eliminated the sole stabilizing N-linked glycosylation site (N249) from select clinically-identified F3 mutants.
35304921	11	92	gly	glycoproteins	1415:1427	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A striking example concerns the involvement of glycan-binding galectins in the regulation of the molecular homeostasis of glycoproteins at the cell surface through the formation of lattices or endocytic pits according to the glycolipid-lectin (GL-Lect) hypothesis.
35281276	3	36	gly	glycosylated	289:300	arg1	Many cellular receptors	Many cellular receptors				Fterm		receptors			Many cellular receptors are glycosylated, and their activity can be modulated through changes in glycan structure.
33006605	7	31	gly	glycosylation	1122:1134	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Moreover, many mutations in N-linked glycosylation sites can increase the stability of the S protein.
35017635	5	19	gly	fucosylated	917:927	arg1	these fucosylated glycans				these fucosylated glycans						Moreover, these fucosylated glycans can serve as ligands for DC-SIGN positive tumour-associated macrophages, modulating their activation and inducing the production of IL-10.
36529080	9	1	gly	glycosylation	1356:1368	arg1	Dectin-1	Dectin-1				PUBTATOR		Dectin-1	64581		we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36529080	9	65	gly	present	1322:1328	arg1	a new N-linked glycosylation site			a new N-linked glycosylation site						site	we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36529080	9	64	gly	glycosylation	1300:1312	arg1	some variants	variants		site		Fterm		variants		site	we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36775128	3	73	gly	possessing	444:453	arg1	a conserved adhesin AND a thrombospondin repeat (TSR) domain	a conserved adhesin			a thrombospondin repeat (TSR) domain	Fterm		adhesin			At present, the only approved vaccine against any apicomplexan parasite targets a conserved adhesin possessing a thrombospondin repeat (TSR) domain.
33952641	2	56	gly	glycoproteins	340:352	arg1	host-encoded, surface-expressed glycoproteins	host-encoded, surface-expressed glycoproteins				Fterm		glycoproteins			One common host-evasion strategy is the downregulation of host-encoded, surface-expressed glycoproteins.
36585837	9	12	gly	glycoproteins	1324:1336	arg1	220 glycoproteins	220 glycoproteins				Fterm		glycoproteins			Shotgun proteome analysis identified 545 N-linked and glutamine, Q, -linked, glycosylation (P>0.75 for deamidation) sites on 220 glycoproteins in sow milk.
36089065	9	41	gly	glycosylation	1771:1783	arg2	each glycosylation site			each glycosylation site						site	These data suggest higher order local structures surrounding each glycosylation site contribute to the efficiency of modification both in vitro and in vivo to establish the spectrum of microheterogeneity in N-linked glycoproteins.
36089065	9	50	gly	glycoproteins	1921:1933	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			These data suggest higher order local structures surrounding each glycosylation site contribute to the efficiency of modification both in vitro and in vivo to establish the spectrum of microheterogeneity in N-linked glycoproteins.
35183770	2	26	gly	glycoproteins	514:526	arg1	distinctive glycoproteins	distinctive glycoproteins				Fterm		glycoproteins			In this study, by mapping the most comprehensive N-glycoproteome of human spermatozoa using our recently developed site-specific glycoproteomic approaches, we show that spermatozoa contain a number of distinctive glycoproteins, which are mainly involved in spermatogenesis, acrosome reaction and sperm:oocyte membrane binding, and fertilization.
36746580	1	58	gly	N-glycosylated	198:211	arg1	Human milk fat globule membrane (MFGM) proteins	Human milk fat globule membrane (MFGM) proteins				Fterm		proteins			Human milk fat globule membrane (MFGM) proteins, which are N-glycosylated, play essential roles in neonatal development and physiological health.
37093892	10	94	gly	glycosylation	1308:1320	arg2	the glycosylation sites			the glycosylation sites						sites	We defined the regions targeted by the nAbs using mutant SU proteins modified at the glycosylation sites, RBD functional subregions, and genotype-specific sequences that present properties of B-cell epitopes.
31941975	9	55	gly	N-glycopeptides	1636:1650	arg2	tri- and tetra-antennary N-glycopeptides			tri- and tetra-antennary N-glycopeptides						N-glycopeptides	Specifically, outer fucosylation was dominant in tri- and tetra-antennary N-glycopeptides, while core fucosylation was dominant in the mono-, bi-antennary and hybrid types of N-glycoproteins in human plasma.
31941975	9	66	gly	N-glycoproteins	1737:1751	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			Specifically, outer fucosylation was dominant in tri- and tetra-antennary N-glycopeptides, while core fucosylation was dominant in the mono-, bi-antennary and hybrid types of N-glycoproteins in human plasma.
33268543	7	62	gly	glycosylation	1054:1066	arg1	N102			N102						N102	This mutation, however, impaired the maturation of NLGN4 protein by inhibiting N-linked glycosylation at an adjacent residue (N102), which is conserved in all NLGNs.
33268543	7	62	gly	glycosylation	1054:1066	arg1	an adjacent residue			an adjacent residue						residue	This mutation, however, impaired the maturation of NLGN4 protein by inhibiting N-linked glycosylation at an adjacent residue (N102), which is conserved in all NLGNs.
31981002	4	6	gly	glycosylation	824:836	arg2	one signal peptide			one signal peptide						peptide	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (74-92%) among various species, retaining one signal peptide, one luminal domain, one serine/threonine kinase domain, one RNase domain, one activation loop, two N-linked glycosylation sites, and several phosphorylation sites.
31981002	4	6	gly	glycosylation	824:836	arg2	two N-linked glycosylation sites			two N-linked glycosylation sites						sites	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (74-92%) among various species, retaining one signal peptide, one luminal domain, one serine/threonine kinase domain, one RNase domain, one activation loop, two N-linked glycosylation sites, and several phosphorylation sites.
35183770	3	11	gly	extracellular	716:728	arg1	regions			regions						regions	Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
35183770	3	15	gly	observed	669:676	arg2	14 glycoproteins AND Heavy fucosylation	14 glycoproteins			Heavy fucosylation	Fterm		glycoproteins			Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
35183770	3	16	gly	glycoproteins	684:696	arg1	14 glycoproteins	14 glycoproteins				Fterm		glycoproteins			Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
35183770	3	47	gly	fucosylation	653:664	arg1	14 glycoproteins	14 glycoproteins				Fterm		glycoproteins			Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
36037649	3	40	gly	glycoprotein	705:716	arg1	the S-layer glycoprotein	the S-layer glycoprotein				Fterm		glycoprotein			In the present report, nuclear magnetic resonance spectroscopy was used to define a tetrasaccharide N-linked to both archaellins, building blocks of the archaeal swimming device (the archaellum), and the S-layer glycoprotein that comprises the protein shell surrounding the Hbt.
32130226	1	29	gly	glycosylation	244:256	arg1	substrate proteins	substrate proteins				Fterm		proteins			The oligosaccharyl transferase (OST) protein complex mediates the N-linked glycosylation of substrate proteins in the endoplasmic reticulum (ER), which regulates stability, activity, and localization of its substrates.
32943699	2	56	gly	glycosylation	458:470	arg1	the Fc region			the Fc region						region	The inflammatory capability of both maternal and neonatal antibodies is governed by N-linked glycosylation of the Fc region, and though this has been examined extensively in adults, there is currently little information regarding antibody glycosylation patterns during early postnatal life.
32356523	0	30	gly	glycoprotein	117:128	arg1	glycoprotein transport	glycoprotein transport				Fterm		glycoprotein			Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport.
34407556	0	42	gly	Asialoglycoprotein	4:21	arg1	The Asialoglycoprotein Receptor	The Asialoglycoprotein Receptor				Fterm		Asialoglycoprotein			The Asialoglycoprotein Receptor Minor Subunit Gene Contributes to Pharmacokinetics of Factor VIII Concentrates in Hemophilia A. BACKGROUND The asialoglycoprotein receptor (ASGPR) binds with high affinity factor VIII (FVIII) through its N-linked oligosaccharides.
34407556	0	76	gly	asialoglycoprotein	143:160	arg1	The asialoglycoprotein receptor	The asialoglycoprotein receptor				Fterm		asialoglycoprotein			The Asialoglycoprotein Receptor Minor Subunit Gene Contributes to Pharmacokinetics of Factor VIII Concentrates in Hemophilia A. BACKGROUND The asialoglycoprotein receptor (ASGPR) binds with high affinity factor VIII (FVIII) through its N-linked oligosaccharides.
36959283	7	74	gly	sialylated	1423:1432	arg1	highly sialylated N-glycans				highly sialylated N-glycans						Furthermore, highly sialylated N-glycans are identified and structurally characterized, and untreated sialic acid-linkage isomers are resolved in a single CZE-MS analysis.
35995381	0	47	gly	N-glycosylation	14:28	arg1	human thyroid thyroglobulin	human thyroid thyroglobulin				PUBTATOR		thyroglobulin	7038		Site-specific N-glycosylation analysis of human thyroid thyroglobulin by mass spectrometry-based Glyco-analytical strategies.
37266972	9	9	gly	site	1193:1196	arg1	the N-glycans			site	the N-glycans					site	More than 90% of the N-glycans at the conserved Asn297 site were deglycosylated.
37266972	9	67	gly	deglycosylated	1203:1216	arg1	the N-glycans				the N-glycans						More than 90% of the N-glycans at the conserved Asn297 site were deglycosylated.
35960654	10	56	gly	glycopeptide	1921:1932	arg2	the enhanced comprehensive quantitative intact glycopeptide analysis			the enhanced comprehensive quantitative intact glycopeptide analysis						glycopeptide	Taken together, we developed and optimized a strategy for the enhanced comprehensive quantitative intact glycopeptide analysis with DiLeu labeling, showing significant promise for identifying novel therapeutic targets or biomarkers in biological systems with a limited sample quantity.
35397991	3	44	gly	GNTI	509:512	arg1	the resultant N-glycan structures	GNTI			the resultant N-glycan structures	OGER		GNTI	P26572		However, the temporal contributions of GNTI to GlcNAc extension and the resultant N-glycan structures in insects have not been analyzed.
36211377	6	20	gly	sialylated	1082:1091	arg1	sialylated N glycans				sialylated N glycans						The most significant alteration of the IgG N-glycome was present 8 weeks after the subjects underwent an LCD, a statistically significant decrease of agalactosylated and the increase of sialylated N glycans.
36252443	12	39	gly	glycoproteins	2109:2121	arg1	glycoproteins' biological roles	glycoproteins' biological roles				Fterm		glycoproteins			EndoBI-1 can be considered a promising enzyme for the release of N-glycans and their protein backbone in the native form, which will enable effective glycan release and will facilitate subsequent investigations to reveal their contribution to glycoproteins' biological roles.
36925056	0	42	gly	N-glycosylation	0:14	arg1	serum proteins	serum proteins				Fterm		proteins			N-glycosylation of serum proteins in adult type 1 diabetes mellitus exposes further changes compared to children at the disease onset.
35871894	7	16	gly	glycoproteins	1081:1093	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In this manuscript, we present a step-by-step workflow to build and perform MD analysis of glycoproteins focusing on the SPIKE glycoprotein of SARS-CoV-2 to appraise the impact of glycans in structure stabilization and antibody occlusion.
35871894	7	27	gly	glycoprotein	1117:1128	arg1	the SPIKE glycoprotein	the SPIKE glycoprotein				PUBTATOR		SPIKE glycoprotein	43740568		In this manuscript, we present a step-by-step workflow to build and perform MD analysis of glycoproteins focusing on the SPIKE glycoprotein of SARS-CoV-2 to appraise the impact of glycans in structure stabilization and antibody occlusion.
34972858	7	107	gly	glycoproteins	1302:1314	arg1	other glycoproteins	other glycoproteins				Fterm		glycoproteins			To address this challenge, we have constructed a detailed InstantPC-labeled glycan glucose unit (GU) database through analysis of human serum and a variety of other glycoproteins from various sources.
36935145	4	24	gly	N-glycosite	1340:1350	arg2	comprehensive N-glycosite			comprehensive N-glycosite						N-glycosite	Here we report our glycoproteome-wide qualitative N-glycoproteomics characterization of GoGs in breast cancer Adriamycin drug resistance (ADR) cells (MCF-7/ADR) and cancer stem cells (MCF-7/ADR CSCs); comprehensive N-glycosite and N-glycan structure information at the intact N-glycopeptide level were reported.
36935145	4	62	gly	N-glycopeptide	1401:1414	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	Here we report our glycoproteome-wide qualitative N-glycoproteomics characterization of GoGs in breast cancer Adriamycin drug resistance (ADR) cells (MCF-7/ADR) and cancer stem cells (MCF-7/ADR CSCs); comprehensive N-glycosite and N-glycan structure information at the intact N-glycopeptide level were reported.
31941975	2	43	gly	glycoproteins	341:353	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Fucosylation of glycoproteins plays an important role for structural stability and function of N-linked glycoproteins.
31941975	2	61	gly	glycoproteins	253:265	arg1	Fucosylation of glycoproteins				Fucosylation of glycoproteins						Fucosylation of glycoproteins plays an important role for structural stability and function of N-linked glycoproteins.
33085102	0	64	gly	glycoproteins	48:60	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Isolation and identification of O- and N-linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth.
35945033	7	10	gly	glycoproteins	1678:1690	arg1	horseradish peroxidase	horseradish peroxidase				Fterm		peroxidase			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	10	gly	glycoproteins	1678:1690	arg1	bovine lactoferrin	bovine lactoferrin				OGER		lactoferrin	P02788		Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	10	gly	glycoproteins	1678:1690	arg1	the glycoproteins horseradish peroxidase and bovine lactoferrin	the glycoproteins horseradish peroxidase and bovine lactoferrin				Fterm		glycoproteins			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	16	gly	deglycosylate	1660:1672	arg1	horseradish peroxidase	horseradish peroxidase				Fterm		peroxidase			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	16	gly	deglycosylate	1660:1672	arg1	bovine lactoferrin	bovine lactoferrin				OGER		lactoferrin	P02788		Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	16	gly	deglycosylate	1660:1672	arg1	the glycoproteins horseradish peroxidase and bovine lactoferrin	the glycoproteins horseradish peroxidase and bovine lactoferrin				Fterm		glycoproteins			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
32417172	1	44	gly	O-glycosylation	183:197	arg1	secretory proteins	secretory proteins				Fterm		proteins			BACKGROUND Mucin-type O-glycosylation (referred to as O-GalNAc glycosylation) is the most abundant O-glycosylation on membrane and secretory proteins.
35320529	2	47	gly	modified	204:211	arg1	Most human lymphocyte membrane receptors AND diverse glycan structures	Most human lymphocyte membrane receptors			diverse glycan structures	Fterm		receptors			Most human lymphocyte membrane receptors are modified by diverse glycan structures, and functional studies have indicated that a family of glycan-binding proteins, galectins, can significantly modulate lymphocyte development and function by interacting with these glycans.
35934376	0	91	gly	glycoprotein	80:91	arg1	ultrafast glycoprotein immobilization	ultrafast glycoprotein immobilization				Fterm		glycoprotein			In-depth profiling of urinary N-glycome in diabetic kidney disease by ultrafast glycoprotein immobilization for glycan extraction (UltraGIG).
34611867	4	26	gly	sialylated	687:696	arg1	galactosylated, agalactosylated, or sialylated glycoforms				galactosylated, agalactosylated, or sialylated glycoforms						Here we describe protocols that can be utilized to produce preferred glycoforms that include galactosylated, agalactosylated, or sialylated glycoforms following isolation of a mAb.
36580234	5	4	gly	sialylated	812:821	arg1	sialylated glycans				sialylated glycans						Results revealed that bisected GlcNAc, core fucosylated, and sialylated glycans (e.g., HexNAc4Hex5Fuc1Neu5Ac1, N4H5F1S1) were increased in M1 and M2 macrophages, especially in the latter.
36233110	5	49	gly	N-glycosylation	1152:1166	arg2	the five N-glycosylation sites			the five N-glycosylation sites						sites	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
36233110	5	52	gly	levels	1070:1075	arg1	the two sites			the two sites	the two sites		Site			sites	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
36233110	5	52	gly	levels	1070:1075	arg1	the VEGFR-2 domain			the VEGFR-2 domain	the VEGFR-2 domain		Site			domain	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
33006605	1	47	gly	glycoprotein	127:138	arg1	The spike (S) glycoprotein	The spike (S) glycoprotein				Fterm		glycoprotein			The spike (S) glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the binding to the permissive cells.
31657490	0	82	gly	glycopeptides	89:101	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	Postsynthesis of zwitterionic hydrophilic composites for enhanced enrichment of N-linked glycopeptides from human serum.
37331167	3	46	gly	glycoprotein	594:605	arg1	a glycoprotein α-1,3 mannosidase	a glycoprotein α-1,3 mannosidase				Fterm		glycoprotein			Through functional genomic analysis, we identified a glycoprotein α-1,3 mannosidase and named it MA3.
36606688	5	52	gly	structure	627:635	arg1	SOD3	SOD3			structure	PUBTATOR		SOD3	6649		We report herein that the fucose structure of the N-glycan in SOD3 was increased in the sera of patients with lung cancer.
36385894	4	92	gly	glycoprotein	617:628	arg1	The N-glycan and glycoprotein profiles				The N-glycan and glycoprotein profiles						The N-glycan and glycoprotein profiles of a cohort (n = 26 patients, n = 22 age- and gender-matched) of lung cancer patients were analyzed and compared to identify potential N-glycan and glycopeptide serum biomarkers using nano-QToF-MS/MS and ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry dynamic multiple monitoring methods, respectively.
35696078	7	19	gly	glycosylation	959:971	arg2	the glycosylation sites			the glycosylation sites						sites	With the development of glycan-related databases and a glycan repository, bioinformatics approaches have attempted to predict the glycosylation pathway and the glycosylation sites on proteins.
36388954	3	2	gly	glycoprotein	738:749	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			The model was able to accommodate site-specific N-glycan processing and we identified phosphorylated glycan structures of the mannose-6-phosphate pathway responsible for the lysosomal sorting of the glycoprotein.
36791651	4	2	gly	polysaccharides	738:752	arg1	CFF	CFF			polysaccharides	OGER		CFF	P51610		In this study, a strategy integrating HPGPC-ELSD, HPLC-PDA, UV-VIS and UPLC-QTOF-MS/MS was firstly developed to simultaneously qualify and quantify polysaccharides, as well as representative small molecules in CFF.
32714760	6	16	gly	glycosylation	759:771	arg1	ZP proteins	ZP proteins				Fterm		proteins			Mutant oocytes have a thin and fragile zona pellucida (ZP) due to the reduction in glycosylation of ZP proteins, and display poor developmental competence after fertilization in vitro.
36478308	12	94	gly	glycosylation	1753:1765	arg1	both spike protein variants	both spike protein variants				Fterm		variants			CONCLUSION Our data clearly shows that the overall glycosylation pattern of both spike protein variants was highly similar from batch-to-batch, and between materials produced at different manufacturing facilities.
36870092	10	7	gly	protein	1577:1583	arg1	bisecting GlcNAc	p38-interacting protein			bisecting GlcNAc	OGER		p38-interacting protein	Q8NEM7		Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36870092	10	17	gly	beta-5	1667:1672	arg1	β1,6-branching N-glycan	integrin beta-5			β1,6-branching N-glycan	OGER		integrin beta-5	P18084		Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36870092	10	24	gly	N-glycopeptide	1519:1532	arg2	down-regulated intact N-glycopeptide			N-glycopeptide	bisecting GlcNAc					N-glycopeptide	Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36870092	10	24	gly	N-glycopeptide	1519:1532	arg2	down-regulated intact N-glycopeptide			N-glycopeptide	β1,6-branching N-glycan					N-glycopeptide	Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36870092	10	41	gly	N-glycopeptide	1609:1622	arg2	up-regulated intact N-glycopeptide			N-glycopeptide	bisecting GlcNAc					N-glycopeptide	Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
34154738	9	23	gly	fucosylation	1409:1420	arg1	the antibody glycan				the antibody glycan						High expressing RMD clones reduced the fucosylation of the antibody glycan to as low as 16%.
36830744	4	45	gly	deglycosylated	724:737	arg1	The purified IgGs	The purified IgGs				Cterm		IgGs			The purified IgGs were denatured and enzymatically deglycosylated, and the released and fluorescently labelled N-glycans were analysed by ultra-high performance liquid chromatography based on hydrophilic interactions with fluorescence detection (HILIC-UHPLC-FLR).
35775947	1	54	gly	glycoproteins	345:357	arg1	the mucin-like glycoproteins	the mucin-like glycoproteins				Fterm		glycoproteins			Two important activities take place in the surface of Trypanosoma cruzi, the agent of Chagas disease: the trans-sialidase (TcTS) catalyzes the transfer of sialic acid from the host glycoconjugates to the mucin-like glycoproteins from the parasite and the presence of lytic antibodies recognize the epitope α-Galp(1 → 3)-β-Galp(1 → 4)-α-GlcNAcp.
35335137	10	45	gly	pro-inflammatory	1224:1239	arg1	the pro-inflammatory tetra-antennary plasma glycans			pro	the pro-inflammatory tetra-antennary plasma glycans					pro	We also detected a substantial increase in the pro-inflammatory tetra-antennary plasma glycans in bilateral AMD (7.90 (2.94-20.95)).
36766693	5	28	gly	mannosylated	1202:1213	arg1	mannosylated glycoproteins	mannosylated glycoproteins				Fterm		glycoproteins			Altered glycan structures composed of mannose residues are expected to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly the entire cascade of events linked to this lectin.
36766693	5	67	gly	glycoproteins	1215:1227	arg1	mannosylated glycoproteins	mannosylated glycoproteins				Fterm		glycoproteins			Altered glycan structures composed of mannose residues are expected to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly the entire cascade of events linked to this lectin.
34778211	8	36	gly	glycopeptides	1332:1344	arg2	7,127 unique N-linked glycosite-containing intact glycopeptides			7,127 unique N-linked glycosite-containing intact glycopeptides						glycopeptides	A total of 7,127 unique N-linked glycosite-containing intact glycopeptides (IGPs), 928 glycosites, and 442 glycoproteins were identified from FUT8KO and WT CHO cells.
34778211	8	49	gly	glycosites	1358:1367	arg2	928 glycosites			928 glycosites						glycosites	A total of 7,127 unique N-linked glycosite-containing intact glycopeptides (IGPs), 928 glycosites, and 442 glycoproteins were identified from FUT8KO and WT CHO cells.
34778211	8	56	gly	glycoproteins	1378:1390	arg1	442 glycoproteins	442 glycoproteins				Fterm		glycoproteins			A total of 7,127 unique N-linked glycosite-containing intact glycopeptides (IGPs), 928 glycosites, and 442 glycoproteins were identified from FUT8KO and WT CHO cells.
35871410	9	72	gly	attached	1360:1367	arg2	invertase AND N-glycans	invertase			N-glycans	Fterm		invertase			In addition, the length of N-glycans attached to invertase was evaluated in the Δmnt1 strain.
35293727	7	83	gly	fragment	1323:1330	arg1	glycan ISD fragment ions				glycan ISD fragment ions						Three glycoconjugate vaccine candidates, obtained from the bioconjugation of the O-antigen polysaccharides from E. coli serotypes O2, O6A, and O25B with the genetically detoxified exotoxin A from Pseudomonas aeruginosa, were analyzed by MALDI-in-source decay (ISD) FT-ICR MS. Protein and glycan ISD fragment ions were selectively detected using 1,5-diaminonaphtalene and a 2,5-dihydroxybenzoic acid/2-hydroxy-5-methoxybenzoic acid mixture (super-DHB) as a MALDI matrix, respectively.
35397991	6	54	gly	enzyme	1390:1395	arg1	the end product N-glycans	enzyme			the end product N-glycans	Fterm		enzyme			The expression profile of GlcNAc extension-related genes revealed that the enzymes contributing to the hydrolysis of GlcNAc showed stage-specific expressions, thereby resulting in accumulations of the end product N-glycans of the enzyme.
34452239	7	63	gly	glycopeptide	1524:1535	arg2	the glycopeptide			the glycopeptide						glycopeptide	Advanced organic solution spray drying was used to engineer the particles and produce spray-dried powders (SD) of both the peptide and the glycopeptide, as well as co-spray-dried powders (co-SD) with the non-reducing sugar and pharmaceutical excipient, trehalose.
36565991	8	26	gly	glycosylation	1191:1203	arg2	viral glycosylation sites			viral glycosylation sites						sites	These observations suggest a mechanism whereby mutations affecting viral glycosylation sites have a structural impact across the protein surface.
37156312	5	45	gly	O-glycosites	729:740	arg2	O-glycosites			O-glycosites						O-glycosites	This study constructed a prediction model based on feature fusion for O-glycosites linked to the threonine residues in Homo sapiens.
36373229	2	7	gly	glycoproteins	324:336	arg1	only glycoproteins	only glycoproteins				Fterm		glycoproteins			O-Glycoprotease substrates range from any O-glycoprotein (albeit with specific O-glycan modifications) to only glycoproteins harboring specific O-glycosylated sequence motifs, such as those found in mucin domains.
36373229	2	60	gly	O-glycoprotein	255:268	arg1	any O-glycoprotein	O-glycoprotein			specific O-glycan modifications	Fterm		O-glycoprotein			O-Glycoprotease substrates range from any O-glycoprotein (albeit with specific O-glycan modifications) to only glycoproteins harboring specific O-glycosylated sequence motifs, such as those found in mucin domains.
36252443	11	77	gly	glycoproteins	1764:1776	arg1	the native glycoproteins	the native glycoproteins				Fterm		glycoproteins			Thirty-two and fifteen distinct compositions were released from the native glycoproteins by EndoBI-1 and PNGase F, respectively, as characterized by advanced mass spectrometry.
37162352	13	66	gly	glycosylation	1701:1713	arg2	the glycosylation sites			the glycosylation sites						sites	The function of O-glycosylation is likely related to protein folding and stability; therefore, the finding of the glycosylation sites has relevance for studies involving expression of the proteins.
36252443	5	92	gly	glycoprotein	843:854	arg1	glycoprotein denaturation	glycoprotein denaturation				Fterm		glycoprotein			However, for conventional enzymatic and chemical deglycosylation strategies to work efficiently, glycoprotein denaturation is required, which alters the protein native shape, hindering further investigations of its biological roles.
35388686	3	40	gly	glycopeptides	734:746	arg2	glycopeptides			glycopeptides						glycopeptides	The method is applied to study glycan compositions based on analysis of glycopeptides from proteolytic digestion of recombinant human coronaviruse spike proteins from SARS-CoV-2 and HKU1.
36903897	4	46	gly	glycoproteins	912:924	arg1	AGP fusion glycoproteins	AGP fusion glycoproteins				Fterm		glycoproteins			In addition, this work confirms the presence of β-1,6-linkage on the galactan backbone identified previously in AGP fusion glycoproteins expressed in tobacco suspension culture.
36746580	5	7	gly	sialylated	989:998	arg1	sialylated branching structures				sialylated branching structures						Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36746580	5	62	gly	core-α1,6-fucosylated	942:962	arg1	core-α1,6-fucosylated structures				core-α1,6-fucosylated structures						Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36746580	5	54	gly	glycoproteins	923:935	arg1	human MFGM glycoproteins	MFGM glycoproteins			Lewis	OGER		MFGM glycoproteins	Q08431		Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36746580	5	54	gly	glycoproteins	923:935	arg1	human MFGM glycoproteins	MFGM glycoproteins			core-α1,6-fucosylated structures	OGER		MFGM glycoproteins	Q08431		Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36746580	5	54	gly	glycoproteins	923:935	arg1	human MFGM glycoproteins	MFGM glycoproteins			sialylated branching structures	OGER		MFGM glycoproteins	Q08431		Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36436856	0	25	gly	glycoproteins	107:119	arg1	human milk glycoproteins	human milk glycoproteins				Fterm		glycoproteins			Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.
36436856	0	36	gly	levels	71:76	arg1	human milk glycoproteins	glycoproteins			levels	Fterm		glycoproteins			Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.
35092134	6	39	gly	glycosylated	908:919	arg1	most sites			most sites						sites	The results indicated that most sites were fully glycosylated and that the dominant population was the complex type.
36972173	6	32	gly	sites	822:826	arg1	the N-terminal domain			the N-terminal domain	the N-terminal domain		Site			domain	However, there are significant differences in the processing state at glycan sites in the N-terminal domain, such as N165.
36290159	9	27	gly	HPA	2836:2838	arg1	O-linked glycans	HPA			O-linked glycans	OGER		HPA	Q9UL45		It can be summarized as follows: (i) high-mannosylated N-linked glycans (Con A reactivity) were present throughout the oviductal epithelium during the entire menstrual cycle and characteristically in the apical protrusions of non-ciliated cells of the ampulla during the preovulatory phase; (ii) sialoglycans with α2,3-linked sialic acids (MAL II binding) were expressed along the entire oviductal surface only during the preovulatory phase, whereas α2,6-linked ones (SNA affinity) were also detected in the surface of the luteal phase, although during the preovulatory phase they were characteristically found in the glycocalyx of the isthmus cilia, and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA) were found in the entire oviductal surface during all phases of the menstrual cycle; (iii) GalNAc terminating O-linked glycans (HPA staining) were mainly expressed in the entire oviducts of the luteal and preovulatory phases, and characteristically in the apical protrusions of the isthmus non-ciliated cells of the preovulatory phase; and (iv) fucosylated glycans with α1,2-linked fucose (LTA reactivity) occurred in the apical surface of fimbriae during the luteal phase, whereas α1,3/4-linked fucose (UEA I binders) were present in the apical protrusions of the ampulla non-ciliated cells and in the apical surface of isthmus during the preovulatory phase as well as in the isthmus apical surface of follicular-phase oviducts.
36290159	9	48	gly	LTA	3098:3100	arg1	α1,2-linked fucose	LTA			α1,2-linked fucose	OGER		LTA	P01374		It can be summarized as follows: (i) high-mannosylated N-linked glycans (Con A reactivity) were present throughout the oviductal epithelium during the entire menstrual cycle and characteristically in the apical protrusions of non-ciliated cells of the ampulla during the preovulatory phase; (ii) sialoglycans with α2,3-linked sialic acids (MAL II binding) were expressed along the entire oviductal surface only during the preovulatory phase, whereas α2,6-linked ones (SNA affinity) were also detected in the surface of the luteal phase, although during the preovulatory phase they were characteristically found in the glycocalyx of the isthmus cilia, and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA) were found in the entire oviductal surface during all phases of the menstrual cycle; (iii) GalNAc terminating O-linked glycans (HPA staining) were mainly expressed in the entire oviducts of the luteal and preovulatory phases, and characteristically in the apical protrusions of the isthmus non-ciliated cells of the preovulatory phase; and (iv) fucosylated glycans with α1,2-linked fucose (LTA reactivity) occurred in the apical surface of fimbriae during the luteal phase, whereas α1,3/4-linked fucose (UEA I binders) were present in the apical protrusions of the ampulla non-ciliated cells and in the apical surface of isthmus during the preovulatory phase as well as in the isthmus apical surface of follicular-phase oviducts.
36290159	9	77	gly	fucosylated	3053:3063	arg1	(iv) fucosylated glycans				(iv) fucosylated glycans						It can be summarized as follows: (i) high-mannosylated N-linked glycans (Con A reactivity) were present throughout the oviductal epithelium during the entire menstrual cycle and characteristically in the apical protrusions of non-ciliated cells of the ampulla during the preovulatory phase; (ii) sialoglycans with α2,3-linked sialic acids (MAL II binding) were expressed along the entire oviductal surface only during the preovulatory phase, whereas α2,6-linked ones (SNA affinity) were also detected in the surface of the luteal phase, although during the preovulatory phase they were characteristically found in the glycocalyx of the isthmus cilia, and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA) were found in the entire oviductal surface during all phases of the menstrual cycle; (iii) GalNAc terminating O-linked glycans (HPA staining) were mainly expressed in the entire oviducts of the luteal and preovulatory phases, and characteristically in the apical protrusions of the isthmus non-ciliated cells of the preovulatory phase; and (iv) fucosylated glycans with α1,2-linked fucose (LTA reactivity) occurred in the apical surface of fimbriae during the luteal phase, whereas α1,3/4-linked fucose (UEA I binders) were present in the apical protrusions of the ampulla non-ciliated cells and in the apical surface of isthmus during the preovulatory phase as well as in the isthmus apical surface of follicular-phase oviducts.
36870092	8	59	gly	N-glycopeptides	1113:1127	arg2	4777 intact N-glycopeptides			4777 intact N-glycopeptides						N-glycopeptides	Totally, 4777 intact N-glycopeptides were identified and N-glycan sequence structures among 2764 IDs were distinguished from their isomers by structure-diagnostic fragment ions.
36870092	8	72	gly	sequence	1158:1165	arg1	N-glycan sequence structures				N-glycan sequence structures						Totally, 4777 intact N-glycopeptides were identified and N-glycan sequence structures among 2764 IDs were distinguished from their isomers by structure-diagnostic fragment ions.
36740532	8	53	gly	O-glycosites	1245:1256	arg2	48 unique O-glycosites			48 unique O-glycosites						O-glycosites	In the analysis of the endogenous protein purified from platelets, we identified 48 unique O-glycosites and 1 N-glycosite.
36740532	8	69	gly	N-glycosite	1264:1274	arg2	1 N-glycosite			1 N-glycosite						N-glycosite	In the analysis of the endogenous protein purified from platelets, we identified 48 unique O-glycosites and 1 N-glycosite.
36959936	7	83	gly	observed	1303:1310	arg1	the mammalian cell culture-derived protein AND the predominantly mature, complex glycans	the mammalian cell culture-derived protein			the predominantly mature, complex glycans	Fterm		protein			Complex-type glycans were almost entirely absent from the plant-derived material, which contrasted against the predominantly mature, complex glycans that were observed on the mammalian cell culture-derived protein.
37371476	5	38	gly	sialylated	1106:1115	arg1	sialylated tri- and tetra-antennary N-glycans				sialylated tri- and tetra-antennary N-glycans						The results from comparing pre-operative CRC to post-operative samples were in agreement with studies that identified a decrease in di-antennary structures with core fucosylation and an increase in sialylated tri- and tetra-antennary N-glycans in CRC patient sera.
37371476	5	42	gly	fucosylation	1074:1085	arg1	di-antennary structures				di-antennary structures						The results from comparing pre-operative CRC to post-operative samples were in agreement with studies that identified a decrease in di-antennary structures with core fucosylation and an increase in sialylated tri- and tetra-antennary N-glycans in CRC patient sera.
35995381	3	18	gly	glycopeptide	576:587	arg2	glycopeptide			glycopeptide						glycopeptide	This study aimed to profile human Tg N-glycosylation sites and their glycan contents by using high-throughput glyco-analytical strategies, including glycopeptide and glycan levels.
35995381	3	101	gly	N-glycosylation	464:478	arg2	human Tg N-glycosylation sites			human Tg N-glycosylation sites						sites	This study aimed to profile human Tg N-glycosylation sites and their glycan contents by using high-throughput glyco-analytical strategies, including glycopeptide and glycan levels.
33119615	5	92	gly	glycosylated	943:954	arg1	mutant G82S glycosylated RAGE variants	mutant G82S glycosylated RAGE variants				OGER		RAGE variants	Q15109		Binding pocket analysis of the MD trajectory showed that cavity/binding pocket in mutant G82S glycosylated RAGE variants is more exposed and accessible to external ligands compared to WT RAGE, which can enhance the affinity of RAGE for Aβ.
36202042	2	50	gly	released	289:296	arg1	proteins AND the N-glycans	proteins			the N-glycans	Fterm		proteins			Various analytical techniques are used for the structural identification of the N-glycans released from proteins through various enzymatic and chemical methods.
37292769	2	4	gly	glycopeptide	267:278	arg2	isobaric glycopeptide structures			isobaric glycopeptide structures						glycopeptide	Despite its immense potential, the qualitative and quantitative analysis of isobaric glycopeptide structures remains one of the most daunting hurdles in the field of glycoproteomics.
37156312	11	63	gly	O-glycosites	1497:1508	arg2	O-glycosites			O-glycosites						O-glycosites	These results demonstrated the high competency of our predictor in identifying O-glycosites on threonine residues.
37071393	1	12	gly	N-glycosylated	148:161	arg1	Many secreted eukaryotic proteins	Many secreted eukaryotic proteins				Fterm		proteins			Many secreted eukaryotic proteins are N-glycosylated with oligosaccharides composed of a high-mannose N-glycan core and, in the specific case of yeast cell-wall proteins, an extended α-1,6-mannan backbone carrying a number of α-1,2- and α-1,3-mannose substituents of varying lengths.
33287410	5	10	gly	glycoproteins	980:992	arg1	the serum glycoproteins	the serum glycoproteins				Fterm		glycoproteins			Whole serum N-glycan profiling was carried out on 117 prostate cancer patients' serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins.
33287410	5	72	gly	released	956:963	arg1	the serum glycoproteins AND N-linked glycans	the serum glycoproteins			N-linked glycans	Fterm		glycoproteins			Whole serum N-glycan profiling was carried out on 117 prostate cancer patients' serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins.
32281790	4	3	gly	glycan	737:742	arg1	the Fc region			the Fc region	the Fc region		Site			region	Specifically, an azide-handle was introduced into the Fc region of a set of immunoglobulins using a two-step enzymatic reaction: (1) cleavage of N-linked glycan in the Fc region by a glycosidase and (2) conjugation of a chemically reactive linker (containing an azide functional handle) using a microbial transglutaminase.
36018613	10	51	gly	glycopeptide	983:994	arg1	the protonated glycopeptide [AT			glycopeptide	the protonated glycopeptide [AT					glycopeptide	Isomerization of the original pyranose, the protonated glycopeptide [AT(GalNAc)A+H]+, is predicted to be facile compared to direct dissociation, as is the glycosidic bond cleavage of the newly formed furanose form, i.e., furanose oxazolinium ion structures are predicted to predominate.
36018613	10	51	gly	glycopeptide	983:994	arg1	GalNAc			glycopeptide	GalNAc					glycopeptide	Isomerization of the original pyranose, the protonated glycopeptide [AT(GalNAc)A+H]+, is predicted to be facile compared to direct dissociation, as is the glycosidic bond cleavage of the newly formed furanose form, i.e., furanose oxazolinium ion structures are predicted to predominate.
36018613	7	47	gly	α-GalNAc-glycosylated	630:650	arg1	threonyl α-GalNAc-glycosylated peptides			threonyl α-GalNAc-glycosylated peptides							In the present article, we test the generality of the preceding findings by examining threonyl α-GalNAc-glycosylated peptides.
36136114	5	76	gly	glycosite	746:754	arg2	a given glycosite			glycosite	the glycan distribution					glycosite	Moreover, the type of glycosylation and the glycan distribution at a given glycosite depend on the host cell line and the expression system conditions that significantly impact safety and efficacy.
34939082	2	36	gly	glycopeptides	278:290	arg2	glycopeptides			glycopeptides						glycopeptides	This problem becomes acute when considering the increasing technical ability to profile large numbers of glycopeptides and glycans in recent studies.
36478308	3	100	gly	glycopeptide	487:498	arg2	glycopeptide libraries			glycopeptide libraries						glycopeptide	The development and use of glycopeptide libraries from previous developmental phases allowed for faster analysis than processing data sets from individual batches from first principles.
35211008	13	110	gly	α-1,6-fucosylation	2411:2428	arg1	isoproterenol-induced cardiac N-linked glycoproteins	isoproterenol-induced cardiac N-linked glycoproteins				Fterm		glycoproteins			Stachytine hydrochloride significantly reduced isoproterenol-induced cardiac N-linked glycoproteins with α-1,6-fucosylation.
35211008	13	121	gly	glycoproteins	2392:2404	arg1	isoproterenol-induced cardiac N-linked glycoproteins	glycoproteins			α-1,6-fucosylation	Fterm		glycoproteins			Stachytine hydrochloride significantly reduced isoproterenol-induced cardiac N-linked glycoproteins with α-1,6-fucosylation.
33789105	6	40	gly	modification	841:852	arg3	TM/ICD AND Decreased O-GlcNAc modification	TM/ICD			Decreased O-GlcNAc modification	PUBTATOR		TM/ICD	79158		Decreased O-GlcNAc modification of TM/ICD increases the binding of E3 ubiquitin ligase Itch to TM/ICD and promotes its degradation.
33789105	6	45	gly	TM/ICD	857:862	arg1	Decreased O-GlcNAc modification	TM/ICD			Decreased O-GlcNAc modification	PUBTATOR		TM/ICD	79158		Decreased O-GlcNAc modification of TM/ICD increases the binding of E3 ubiquitin ligase Itch to TM/ICD and promotes its degradation.
33789105	5	39	gly	fragment	812:819	arg1	the O-GlcNAc modification			fragment	the O-GlcNAc modification					fragment	Mechanistic studies show that Ogt directly interacts with Notch1 and catalyzes the O-GlcNAc modification of Notch TM/ICD fragment.
34952005	6	73	gly	glycosylated	1168:1179	arg1	mG6PC1	mG6PC1				PUBTATOR		G6PC1	2538		When purified from Sf9 insect cell membranes, the glycosylated mouse ortholog (mG6PC1) recapitulated functional properties observed previously in intact hepatic microsomes and displayed the highest specific activity reported to date.
37121976	1	37	gly	glycoprotein	105:116	arg1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1				Fterm		glycoprotein			The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
37121976	1	46	gly	ɑ-2-glycoprotein	131:146	arg1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1				Fterm		ɑ-2-glycoprotein 1			The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
37121976	1	46	gly	ɑ-2-glycoprotein	131:146	arg1	LRG1	LRG1				OGER		LRG1	P02750		The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
37121976	1	46	gly	ɑ-2-glycoprotein	131:146	arg1	a multifunctional protein	a multifunctional protein				Fterm		protein			The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
35604098	3	46	gly	glycosylated	544:555	arg1	cholera toxin B-subunit	cholera toxin B-subunit				PUBTATOR		cholera toxin B-subunit	9468		In this work, we designed and synthesized an intentionally glycosylated cholera toxin B-subunit (CTB) to be transported to the organelles of mammalian cells.
35604098	3	46	gly	glycosylated	544:555	arg1	CTB	CTB				PUBTATOR		CTB	9468		In this work, we designed and synthesized an intentionally glycosylated cholera toxin B-subunit (CTB) to be transported to the organelles of mammalian cells.
36253324	4	2	gly	enabling	817:824	arg1	intact LacNAc sites			intact LacNAc sites						sites	This strategy takes advantage of an operationally simple oxidation enzyme module by using galactose oxidase (GOase) to convert the LacNAc unit into oxidized C6'-aldehyde LacNAc sequence, which is not a good substrate for recombinant α1,3-FucT from Helicobacter pylori strain 26695 (Hpα1,3FucT), enabling the site-specific α1,3-fucosylation at intact LacNAc sites.
36253324	4	19	gly	strain	790:795	arg1	recombinant α1,3-FucT	strain			recombinant α1,3-FucT	Fterm		strain			This strategy takes advantage of an operationally simple oxidation enzyme module by using galactose oxidase (GOase) to convert the LacNAc unit into oxidized C6'-aldehyde LacNAc sequence, which is not a good substrate for recombinant α1,3-FucT from Helicobacter pylori strain 26695 (Hpα1,3FucT), enabling the site-specific α1,3-fucosylation at intact LacNAc sites.
36233110	2	28	gly	N-glycosylation	491:505	arg2	the five N-glycosylation sites			the five N-glycosylation sites						sites	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
36233110	2	35	gly	sites	507:511	arg1	one site			one site						site	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
36233110	2	35	gly	sites	507:511	arg1	two sites			sites						sites	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
37037133	5	33	gly	N-glycoproteins	905:919	arg1	HM MFGM N-glycoproteins	HM MFGM N-glycoproteins				OGER		HM MFGM N-glycoproteins	Q08431		Bioinformatics was used to describe the structure-activity relationships of DC, DM, HC, and HM MFGM N-glycoproteins.
37354227	8	72	gly	glycopeptides	1608:1620	arg2	glycopeptides			glycopeptides						glycopeptides	Additionally, we applied multiple digestion methods on each sample, and confirmed the results for individual glycosylation sites from different experiment conditions to improve the identification and quantification of glycopeptides.
37354227	8	102	gly	glycosylation	1499:1511	arg2	individual glycosylation sites			individual glycosylation sites						sites	Additionally, we applied multiple digestion methods on each sample, and confirmed the results for individual glycosylation sites from different experiment conditions to improve the identification and quantification of glycopeptides.
32820167	5	10	gly	positioned	669:678	arg1	O-glycans			position	O-glycans					position	We demonstrate that O-glycans positioned within the receptor binding motifs of members of the neuropeptide Y and glucagon families modulate receptor activation properties and substantially extend peptide half-lives.
35397991	1	38	gly	N-glycosylation	114:128	arg1	proteins	proteins				Fterm		proteins			N-glycosylation of proteins is an important post-translational modification in eukaryotic cells.
35616904	6	69	gly	glycoproteins	860:872	arg1	identified glycoproteins	identified glycoproteins				Fterm		glycoproteins			The majority of identified glycoproteins functioned in response to stimulus and immunity.
35939326	9	56	gly	epitope	1584:1590	arg1	the inner core trisaccharide key epitope				the inner core trisaccharide key epitope						The phosphate group of the inner core trisaccharide key epitope is very important for IgG recognition.
32898162	7	25	gly	domain	1160:1165	arg1	the second glycan			domain	the second glycan					domain	We propose that residues 227 and 230 and the second glycan are part of a C-terminal domain that acts as a linchpin for bank vole and mouse prion conversion.
34029329	5	48	gly	glycosylation	911:923	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	The amino acid sequences were assessed for their phylogenetic association, variable loop lengths and prevalence of potential N-linked glycosylation sites (pNLGS).
33231560	9	56	gly	RIPK3	1887:1891	arg1	OGT mediated O-GlcNAcylation	RIPK3			OGT mediated O-GlcNAcylation	PUBTATOR		RIPK3	246240		However, OSMI-1, an OGT inhibitor, abolished SPC mediated cardioprotective effects and inhibited OGT mediated up-regulation of O-GlcNAcylation and down-regulation of RIPK3 and MLKL proteins induced by SPC. Our study demonstrated that SPC restrained MIRI induced necroptosis via regulating OGT mediated O-GlcNAcylation of RIPK3 and lessening the formulation of RIPK3/MLKL complex.
32417172	6	85	gly	glycosylation	1053:1065	arg1	p53	p53			glycosylation	OGER		p53	P04637		METHODS Using lectin blotting with GalNAc specific lectins, enzymatic treatments with O-GlcNAcase, core 1 β1, 3-galactosyltransferase and O-glycosidase, and metabolic labeling with un-O-acetylated GalNAz in UDP-Gal/UDP-GalNAc 4-epimerase (GALE) knockout cells, we validated the O-GalNAc glycosylation on p53.
34804021	5	76	gly	IgG	1001:1003	arg1	N-glycan biosynthesis	IgG			N-glycan biosynthesis	PUBTATOR		IgG	16059		Furthermore, pathway enrichment showed several IgG N-glycosylation-related pathways, such as asparagine N-linked glycosylation, N-glycan biosynthesis and transport to the Golgi and subsequent modification.
36244450	0	85	gly	N-glycosylation	0:14	arg1	mannose receptor	CD206			mannose receptor	PUBTATOR		CD206	4360		N-glycosylation of mannose receptor (CD206) regulates glycan binding by C-type lectin domains.
36598201	3	51	gly	N-glycosylation	429:443	arg2	two conserved N-glycosylation sites			two conserved N-glycosylation sites						sites	NSP4, a multifunctional protein involved in various viral replication processes, has two conserved N-glycosylation sites; however, the role of glycans remains elusive.
36239409	6	66	gly	NN015840T	1501:1509	arg1	the acidic O-specific polysaccharide	NN015840T			the acidic O-specific polysaccharide	Cterm		NN015840T			The structure of the acidic O-specific polysaccharide from Cellulophaga baltica strain NN015840T differs to that of the O-antigen from E. coli O93 by lacking the O-acetyl group at O6 of the O-acetylated mannosyl residue.
37156312	3	57	gly	O-glycosites	517:528	arg2	O-glycosites			O-glycosites						O-glycosites	Biochemical experiments to identify O-glycosites in batches are technically and economically demanding.
36838558	8	12	gly	glycosylated	1105:1116	arg1	the glycosylated peptides			the glycosylated peptides						peptides	Furthermore, structural information regarding the glycosylation site and glycan structure was obtained using tandem mass spectrometric (MS/MS) analysis of the glycosylated peptides after tryptic digestion.
36838558	8	39	gly	glycosylation	996:1008	arg2	the glycosylation site			the glycosylation site						site	Furthermore, structural information regarding the glycosylation site and glycan structure was obtained using tandem mass spectrometric (MS/MS) analysis of the glycosylated peptides after tryptic digestion.
36637420	0	79	gly	N-glycosylation	0:14	arg1	Asn-57	HAI-2 protein		Asn-57				HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	protease inhibitory activity	protease		Asn-57		Fterm		protease		Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	protease inhibitory activity	protease		Asn-57		Fterm		protease		Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein				PUBTATOR		HAI-2 protein	10653		N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
32273875	6	58	gly	removal	1175:1181	arg3	mannose-ending glycans AND N2118			N2118	mannose-ending glycans			FVIII	14069	N2118	However, removal of mannose-ending glycans at N2118 did not alter factor VIII endocytosis and presentation to CD4+ T cells by mouse antigen-presenting cells.
36813234	7	24	gly	glycoproteins	1279:1291	arg1	two structurally distinct glycoproteins	two structurally distinct glycoproteins				Fterm		glycoproteins			We found that the expression of GnT-III consistently decreased chemoresistance for doxorubicin and dasatinib, as well as activation of the NF-κB pathway by tumor necrosis factor α (TNFα), which binds to two structurally distinct glycoproteins, TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2), on the cell surface.
36813234	7	24	gly	glycoproteins	1279:1291	arg1	TNF receptor 1	TNF receptor 1				OGER		TNF receptor 1	P01375		We found that the expression of GnT-III consistently decreased chemoresistance for doxorubicin and dasatinib, as well as activation of the NF-κB pathway by tumor necrosis factor α (TNFα), which binds to two structurally distinct glycoproteins, TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2), on the cell surface.
36813234	7	24	gly	glycoproteins	1279:1291	arg1	TNF receptor 2	TNF receptor 2				OGER		TNF receptor 2	P01375		We found that the expression of GnT-III consistently decreased chemoresistance for doxorubicin and dasatinib, as well as activation of the NF-κB pathway by tumor necrosis factor α (TNFα), which binds to two structurally distinct glycoproteins, TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2), on the cell surface.
35848837	6	47	gly	attached	958:965	arg2	their target proteins AND asparagine (Asn)-linked and free glycans	their target proteins			asparagine (Asn)-linked and free glycans	Fterm		proteins			In this article, we present a method that can characterize the N-linked glycosylation system in Campylobacter jejuni by detecting asparagine (Asn)-linked and free glycans that are not attached to their target proteins.
36812846	2	17	gly	glycoproteins	327:339	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Recently, free oligomannose-type glycans generated by the hydrolysis of glycoproteins or dolichol pyrophosphate-linked oligosaccharides were recognized as important signals for immunogenicity.
31941975	4	23	gly	N-glycopeptides	637:651	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Here, we report for the first time the classification of N-glycopeptides as core- and outer-fucosylated types using tandem mass spectrometry (MS/MS) and machine learning algorithms such as the deep neural network (DNN) and support vector machine (SVM).
31941975	4	56	gly	outer-fucosylated	666:682	arg1	outer-fucosylated types				outer-fucosylated types						Here, we report for the first time the classification of N-glycopeptides as core- and outer-fucosylated types using tandem mass spectrometry (MS/MS) and machine learning algorithms such as the deep neural network (DNN) and support vector machine (SVM).
37319314	3	12	gly	glycopeptide	651:662	arg2	glycopeptide isomer ratios			glycopeptide isomer ratios						glycopeptide	Here, we developed an LC-MS/MS-based workflow for determining glycopeptide isomer ratios.
37202422	3	19	gly	glycoproteins	491:503	arg1	highly-related, but non-equivalent glycoproteins	highly-related, but non-equivalent glycoproteins				Fterm		glycoproteins			The variable construction of this glycan structure leads to highly-related, but non-equivalent glycoproteins known as glycoforms.
33657401	5	30	gly	protein	867:873	arg1	O-GlcNAcylation-dependent binding	protein			O-GlcNAcylation-dependent binding	Fterm		protein			We determined that protein nuclear import by O-GlcNAcylation-dependent binding of cargo protein to importin α might be a general mechanism in cells.
34911982	0	67	gly	afucosylated	35:46	arg1	afucosylated IgG	afucosylated IgG				PUBTATOR		IgG	668542		A functional spleen contributes to afucosylated IgG in humans.
36968546	8	21	gly	sialylated	1277:1286	arg1	a sialylated trisaccharide				a sialylated trisaccharide						Similarly, O-glycosylation with a sialylated trisaccharide had a negative impact on TMPRSS2 cleavage.
35304921	8	77	gly	found	984:988	arg1	β1 integrin AND An O-linked HexNAc	integrin		domain	An O-linked HexNAc	Fterm		integrin		domain	An O-linked HexNAc was found in an EGF-like domain of β1 integrin.
33801653	1	20	gly	attachment	203:212	arg2	Ser/Thr residues AND GlcNAc			Ser/Thr residues	GlcNAc					residues	Protein O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine (GlcNAc) to the hydroxyl groups of Ser/Thr residues on numerous nucleocytoplasmic proteins.
33801653	1	20	gly	attachment	203:212	arg2	Ser/Thr residues AND N-acetylglucosamine			Ser/Thr residues	N-acetylglucosamine					residues	Protein O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine (GlcNAc) to the hydroxyl groups of Ser/Thr residues on numerous nucleocytoplasmic proteins.
36959210	2	44	gly	glycoproteins	302:314	arg1	gel-forming glycoproteins	gel-forming glycoproteins				Fterm		glycoproteins			One important, but understudied, factor is the natural host environment: mucus, which contains gel-forming glycoproteins (mucins) that display hundreds of glycan structures with potential regulatory function.
35802832	1	21	gly	glycopeptide	275:286	arg2	the glycopeptide			the glycopeptide						glycopeptide	Casein glycomacropeptide carries various O-glycan modifications, which, together with variations in the amino acid composition of the glycopeptide, may result in different biological activities.
35802832	1	25	gly	carries	166:172	arg1	Casein glycomacropeptide AND various O-glycan modifications			Casein glycomacropeptide	various O-glycan modifications					glycomacropeptide	Casein glycomacropeptide carries various O-glycan modifications, which, together with variations in the amino acid composition of the glycopeptide, may result in different biological activities.
35766466	3	71	gly	glycoproteins	563:575	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			A novel MALDI imaging mass spectrometry workflow has been recently developed to analyze these features of N-glycosylation through the utilization of endoglycosidase enzymes to cleave N-glycans from associated glycoproteins.
37162352	14	34	gly	O-glycosylated	1867:1880	arg1	Six proteins	Six proteins				Fterm		proteins			Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated, which may impact the structure and function of these components.
36135182	9	5	gly	N-glycosylation	1123:1137	arg1	DSPAα2	DSPAα2				PUBTATOR		DSPAα2	112321404		This study confirms that N-glycosylation affects the biochemical function of DSPAα2, which provides a reference for subsequent applications of DSPAα2.
36585837	11	77	gly	glycosylated	1616:1627	arg1	the most glycosylated protein	the most glycosylated protein				Fterm		protein			PIGR (polymeric immunoglobulin receptor) was the most glycosylated protein with 14 sites identified.
36585837	11	77	gly	glycosylated	1616:1627	arg1	PIGR	PIGR				PUBTATOR		PIGR	397315		PIGR (polymeric immunoglobulin receptor) was the most glycosylated protein with 14 sites identified.
36985724	6	43	gly	glycoproteins	1110:1122	arg1	therapeutic glycoproteins	glycoproteins			N-glycan engineering	Fterm		glycoproteins			The platform can find broad applications in N-glycan engineering of therapeutic glycoproteins.
35370997	14	29	gly	N-glycosylation	3054:3068	arg1	IgG	IgG				Cterm		IgG			Our results suggest that N-glycosylation of IgG undergoes dynamic changes during the intensification of thyroiditis in HT, and that in GD autoimmunity it is affected significantly by immunosuppressive therapy.
36924942	5	6	gly	glycosylated	883:894	arg1	a conserved oligosaccharide core				a conserved oligosaccharide core						The N-terminal and C-terminal modules add alternating Kdo residues with β-(2→4) and β-(2→7) linkages, respectively, generating a conserved oligosaccharide core that is further glycosylated to produce diverse capsule structures.
35854001	2	7	gly	branch	348:353	arg1	the α1-3 mannose arm			the α1-3 mannose arm	the α1-3 mannose arm						N-Acetylglucosaminyltransferase-IVa (GnT-IVa, also designated as MGAT4A) forms a β1-4 GlcNAc branch on the α1-3 mannose arm in N-glycans.
35123071	7	61	gly	glycoproteins	1386:1398	arg1	bisecting GlcNAc modifications	glycoproteins			bisecting GlcNAc modifications	Fterm		glycoproteins			These results provide insights into the potential roles of bisecting GlcNAc modification in the human amniotic membrane, and can be beneficial to functional studies on glycoproteins with bisecting GlcNAc modifications and functional studies on immune suppression in human placenta.
37121976	2	18	gly	composition	455:465	arg1	LRG1	LRG1			composition	OGER		LRG1	P02750		Serum LRG1 and neutrophil-derived LRG1 have different molecular weights due to differences in glycosylation, but the impact of the differential glycan composition in LRG1 on its cellular function is largely unknown.
36311785	2	28	gly	glycoprotein	467:478	arg1	yeast glycoprotein	yeast glycoprotein				Fterm		glycoprotein			In our previous study, we reported that supplementation of Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG) in combination synergistically improved antioxidant activities, serum lipid profile, and fatty acid metabolism in chicken.
32691273	5	71	gly	proteins	864:871	arg1	the O-GlcNAcylation	proteins			the O-GlcNAcylation	Fterm		proteins			First, we treated cells with glucosamine and PUGNAc to stimulate the O-GlcNAcylation of total proteins.
35995381	6	50	gly	20 N-glycosylation	1013:1030	arg2	20 N-glycosylation sites			20 N-glycosylation sites						sites	In total, 17 out of 20 N-glycosylation sites were characterized.
36827096	1	25	gly	glycoproteins	255:267	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			C-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders.
34186233	1	47	gly	residues	354:361	arg1	a single β-N-acetylglucosamine (GlcNAc) moiety			residues	a single β-N-acetylglucosamine (GlcNAc) moiety					residues	O-GlcNAcylation is a dynamic post-translational modification mediated by O-linked β-N-acetylglucosamine transferase (OGT) and O-GlcNAc hydrolase (OGA), that adds or removes a single β-N-acetylglucosamine (GlcNAc) moiety to or from serine/threonine residues of nucleocytosolic and mitochondrial proteins, respectively.
35211008	0	8	gly	N-Glycosylation	130:144	arg1	β1AR	β1AR				PUBTATOR		1	12044		Stachytine Hydrochloride Improves Cardiac Function in Mice with ISO-Induced Heart Failure by Inhibiting the α-1,6-Fucosylation on N-Glycosylation of β1AR.
34012659	11	85	gly	non-N-glycosylated	1444:1461	arg1	BST-2	BST-2				PUBTATOR		BST-2	684		We also observed the increased ER degradation-enhancing α-mannosidase-like protein 3 (EDEM3), which is trimming of N-linked glycans by sequential removal of mannose residues, might result in more non-N-glycosylated form of BST-2.
37354227	7	28	gly	types	1295:1299	arg1	later variants	variants			types	Fterm		variants			In comparison with our previously reported site-specific glycan analysis on the S-D614G and its ancestral protein, glycan types on later variants showed high similarity on the site-specific glycan content to S-D614G.
33244686	5	28	gly	glycopeptides	1317:1329	arg2	glycopeptides			glycopeptides						glycopeptides	Here, we reported an optimized experimental condition for IP-HILIC-MS where glycine is added in the TFA-containing mobile phases to enhance the MS detection sensitivity for glycopeptides up to ~ 50-fold by eliminating the ion-suppression effect of an ion-pairing agent while still retaining excellent separation capacity.
36740532	4	48	gly	glycosylation	610:622	arg2	GPIbα amino acid sites			GPIbα amino acid sites						sites	OBJECTIVES The aim of this study was to comprehensively analyze GPIbα amino acid sites of glycosylation (glycosites) and glycan structures.
36740532	4	51	gly	glycosites	625:634	arg2	glycosites			glycosites						glycosites	OBJECTIVES The aim of this study was to comprehensively analyze GPIbα amino acid sites of glycosylation (glycosites) and glycan structures.
32340215	1	4	gly	glycoprotein	172:183	arg1	MAG	MAG				PUBTATOR		MAG	4099		Homozygous variants in MAG, encoding myelin-associated glycoprotein (MAG), have been associated with complicated forms of hereditary spastic paraplegia (HSP).
32340215	1	4	gly	glycoprotein	172:183	arg1	encoding myelin-associated glycoprotein	glycoprotein (MAG)				PUBTATOR		glycoprotein (MAG)	4099		Homozygous variants in MAG, encoding myelin-associated glycoprotein (MAG), have been associated with complicated forms of hereditary spastic paraplegia (HSP).
35504880	4	1	gly	glycopeptides	571:583	arg2	the glycopeptides			the glycopeptides						glycopeptides	The binding of the glycopeptides to the enzyme is mainly driven by the GalNAc moiety while the peptide sequence provides optimal kinetic and binding parameters.
36014368	7	70	gly	N-glycosylation	1002:1016	arg1	the S protein	the S protein				PUBTATOR		S protein	Q15517		In this work, the N-glycosylation status of the S protein within virus-like particles (VLPs) produced in Nicotiana benthamiana (N. benthamiana) was investigated using a glycoproteomic approach.
36311785	0	81	gly	glycoprotein	173:184	arg1	Yeast glycoprotein	Yeast glycoprotein				Fterm		glycoprotein			Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens.
34626388	6	65	gly	fucosylated	1426:1436	arg1	novel fucosylated GAG structures				novel fucosylated GAG structures						Moreover, fucose was detected in chondroitin sulfate, dermatan sulfate, and heparan sulfate, and both fucose and mannose were detected in chondroitin sulfate, suggesting the co-presence of other types of glycans or novel fucosylated GAG structures.
34626388	5	53	gly	observed	1093:1100	arg2	heparin AND galactosamine	heparin			galactosamine	Fterm		heparin			Glucosamine was found in both chondroitin sulfate and dermatan sulfate whereas galactosamine was observed in both heparan sulfate and heparin, indicating the cross contamination among different types of GAGs.
36095053	5	11	gly	N79	756:758	arg1	the N-glycans			N79	the N-glycans			TREM2	54209	N79	We show that the N-glycans at N79 have a requisite role in translocation of TREM2 to the cell surface, while the N-glycans at both N20 and N79 have a critical role in intracellular signal transduction.
36095053	5	45	gly	N79	865:867	arg1	the N-glycans			N20 and N79	the N-glycans			TREM2	54209	N20 and N79	We show that the N-glycans at N79 have a requisite role in translocation of TREM2 to the cell surface, while the N-glycans at both N20 and N79 have a critical role in intracellular signal transduction.
36095053	5	53	gly	N20	857:859	arg1	the N-glycans			N20 and N79	the N-glycans			TREM2	54209	N20 and N79	We show that the N-glycans at N79 have a requisite role in translocation of TREM2 to the cell surface, while the N-glycans at both N20 and N79 have a critical role in intracellular signal transduction.
37287875	3	11	gly	N-glycoproteins	458:472	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			StrucGP is a glycan database-independent search engine for the structural interpretation of N-glycoproteins at the site-specific level.
35452121	2	48	gly	glycosylation	464:476	arg1	proteins	proteins				Fterm		proteins			Recent glycobiological research is painting a picture in which glycans represent a crucial structural and functional component of the majority of proteins, with alternative glycosylation of proteins and lipids being an important regulatory mechanism in many biological and pathological processes.
36870092	6	47	gly	N-glycopeptides	697:711	arg2	differential cell-surface intact N-glycopeptides			differential cell-surface intact N-glycopeptides						N-glycopeptides	Here, differential cell-surface intact N-glycopeptides in adriamycin (ADR)-resistant michigan breast cancer foundation-7 stem cells (MCF-7/ADR CSCs) relative to ADR-sensitive MCF-7 CSCs were analyzed with site- and structure-specific quantitative N-glycoproteomics.
33686291	3	33	gly	deglycosylation	457:471	arg1	a target protein	a target protein				Fterm		protein			Herein, we developed a nanobody-fused split O-GlcNAcase (OGA) as an O-GlcNAc eraser for selective deglycosylation of a target protein in cells.
34422587	1	2	gly	sialylated	169:178	arg1	mucin-type sialylated O-linked glycans				mucin-type sialylated O-linked glycans						BACKGROUND A recent study showed that mucin-type sialylated O-linked glycans could induce the increased expression of PD-L1 via binding to Siglec receptors.
36089065	2	26	gly	glycoprotein	434:445	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			N-glycans are processed in the secretory pathway to form varied ensembles of structures, and diversity at a single site on a glycoprotein is termed 'microheterogeneity'.
34274643	9	70	gly	glycoproteins	1558:1570	arg1	other intact glycoproteins	other intact glycoproteins				Fterm		glycoproteins			The separation and analysis methods that were developed could be used with relatively small amounts of AGP and can be adapted for use with other intact glycoproteins.
37156312	6	47	gly	glycosites	899:908	arg2	O-linked threonine glycosites			O-linked threonine glycosites						glycosites	In the training model, we collected and sorted out high-quality human protein data with O-linked threonine glycosites.
35604098	1	15	gly	glycoproteins	233:245	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycosylation of proteins is known to be essential for changing biological activity and stability of glycoproteins on the cell surfaces and in body fluids.
35604098	1	33	gly	Glycosylation	132:144	arg1	proteins	proteins				Fterm		proteins			Glycosylation of proteins is known to be essential for changing biological activity and stability of glycoproteins on the cell surfaces and in body fluids.
36329887	0	49	gly	Asparagine<sup>52</sup>	30:52	arg1	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans			Asparagine	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans					Asparagine	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans: Major Glycan Structural Consistency, Minor Glycan Variation in Abundance.
36329887	0	49	gly	Asparagine<sup>52</sup>	30:52	arg1	Minor Glycan Variation			Asparagine	Minor Glycan Variation					Asparagine	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans: Major Glycan Structural Consistency, Minor Glycan Variation in Abundance.
36746580	3	20	gly	glycoproteins	546:558	arg1	221 MFGM glycoproteins	221 MFGM glycoproteins			986 unique site-specific N-glycans	OGER		221 MFGM glycoproteins	Q08431		Therefore, in this study, based on an intact glycopeptide-centred strategy, 2617 unique site-specific N-glycans of 221 MFGM glycoproteins in human colostrum and 986 unique site-specific N-glycans of 200 MFGM glycoproteins in mature milk were characterised and quantified using label-free glycoproteomics.
36746580	3	20	gly	glycoproteins	546:558	arg1	221 MFGM glycoproteins	221 MFGM glycoproteins			2617 unique site-specific N-glycans	OGER		221 MFGM glycoproteins	Q08431		Therefore, in this study, based on an intact glycopeptide-centred strategy, 2617 unique site-specific N-glycans of 221 MFGM glycoproteins in human colostrum and 986 unique site-specific N-glycans of 200 MFGM glycoproteins in mature milk were characterised and quantified using label-free glycoproteomics.
36746580	3	16	gly	glycoproteins	630:642	arg1	986 unique site-specific N-glycans	200 MFGM glycoproteins			986 unique site-specific N-glycans	OGER		200 MFGM glycoproteins	Q08431		Therefore, in this study, based on an intact glycopeptide-centred strategy, 2617 unique site-specific N-glycans of 221 MFGM glycoproteins in human colostrum and 986 unique site-specific N-glycans of 200 MFGM glycoproteins in mature milk were characterised and quantified using label-free glycoproteomics.
36746580	3	16	gly	glycoproteins	630:642	arg1	2617 unique site-specific N-glycans	200 MFGM glycoproteins			2617 unique site-specific N-glycans	OGER		200 MFGM glycoproteins	Q08431		Therefore, in this study, based on an intact glycopeptide-centred strategy, 2617 unique site-specific N-glycans of 221 MFGM glycoproteins in human colostrum and 986 unique site-specific N-glycans of 200 MFGM glycoproteins in mature milk were characterised and quantified using label-free glycoproteomics.
34691043	9	51	gly	cysteine	1269:1276	arg1	glycan engineering			cysteine	glycan engineering					cysteine	Thus, glycan engineering and cysteine stabilization are two strategies that can be used together to alter immunodominance patterns to HA.
35782731	6	57	gly	glycoprotein	874:885	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			Since the mentioned central region in the glycoprotein is not surrounded by glycans and is close to the heme, it is easily approachable to the solvent and substrate.
35506863	7	72	gly	fucosylated	837:847	arg1	complex and outer-arm fucosylated glycans				complex and outer-arm fucosylated glycans						Following rat SCI, complex and outer-arm fucosylated glycans decreased while oligomannose and hybrid structures increased.
33381907	2	60	gly	glycosylation	303:315	arg1	a cysteine			cysteine						cysteine	Bang-25 consists of the first 25 amino acids of the N-terminus of angiotensinogen (Aogen), with N-linked glycosylation on the 14th amino acid and a cysteine conjugated to the 18th amino acid.
36716113	6	84	gly	IgG1	1237:1240	arg1	maximal, minimally opposed, pro-inflammatory glycan profiles	IgG1			maximal, minimally opposed, pro-inflammatory glycan profiles	OGER		IgG1	P01857		In contrast, patients with antibiotic-refractory LA, pre-IV therapy, had total IgG1 and Bb-IgG1 antibodies with maximal, minimally opposed, pro-inflammatory glycan profiles, containing high percentages of GlcNAc and bisecting GlcNAc, intermediate percentages with galactose and fucose, and low percentages with NeuAC (sialic acid).
35616904	2	81	gly	glycoproteins	268:280	arg1	these glycoproteins	these glycoproteins				Fterm		glycoproteins			However, the detailed information on these glycoproteins, particularly site-specific glycan structures, is still limited.
35616904	1	0	gly	glycoproteins	91:103	arg1	N-Linked glycoproteins	N-Linked glycoproteins				Fterm		glycoproteins			N-Linked glycoproteins are rich in seminal plasma, playing various essential roles in supporting sperm function and the fertilization process.
34911982	3	29	gly	region	618:623	arg1	N-linked glycans			region	N-linked glycans					region	During both types of immunizations, antigen-specific immunoglobulins G (IgGs) have been characterized by the reduced amount of fucose present on N-linked glycans of the fragment crystallizable (Fc) region.
36095053	2	46	gly	glycosylated	288:299	arg1	TREM2	TREM2				PUBTATOR		TREM2	54209		TREM2 is glycosylated in vitro and in vivo, but the significance of the modification is unknown.
35370997	3	13	gly	attached	630:637	arg1	the Fc fragment AND N-glycans			the Fc fragment	N-glycans					fragment	The IgG structure and its effector functions are strongly dependent on the composition of N-glycans attached to the Fc fragment, and the binding of antigens is regulated by Fab sugar moieties.
36781790	6	29	gly	glycosylation	1495:1507	arg2	site			site						site	Benefits of the glycomics-assisted glycoproteomics method, which can be readily applied to both simple and complex biological specimens such as protein extracts from cells, tissues, and bodily fluids (e.g., serum), include quantitative information of the protein carriers and site(s) of glycosylation, site occupancy, and the site-specific glycan structures directly from biological samples.
35662639	6	33	gly	glycoprotein	1574:1585	arg1	the glycoprotein subproteomes	the glycoprotein subproteomes				Fterm		glycoprotein			The different contents of lectin-enriched proteins underscore novel aspects of the variability of the glycoprotein subproteomes of Bothrops venoms and point to the role of distinct types of glycan chains in generating different venoms by closely related snake species.
35960654	2	28	gly	glycopeptides	420:432	arg2	glycopeptides			glycopeptides						glycopeptides	However, mass spectrometry (MS)-based glycoproteomic analysis is hindered by the low abundance and poor ionization efficiency of glycopeptides.
33904933	7	67	gly	glycosylated	1467:1478	arg1	the glycosylated epitope			the glycosylated epitope						epitope	Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, si-RNA silencing analysis, enzymatic deglycosylation, and tunicamycin treatment revealed that 12G5A recognized the glycosylated epitope on annexin A2 protein.
36014368	4	0	gly	glycosylated	613:624	arg1	The SARS-CoV-2 S protein	The SARS-CoV-2 S protein				PUBTATOR		S protein	Q15517		The SARS-CoV-2 S protein is heavily glycosylated with 22 predicted N-glycosylation consensus sites as well as numerous mucin-type O-glycosylation sites.
36014368	4	7	gly	O-glycosylation	707:721	arg2	numerous mucin-type O-glycosylation sites			numerous mucin-type O-glycosylation sites						sites	The SARS-CoV-2 S protein is heavily glycosylated with 22 predicted N-glycosylation consensus sites as well as numerous mucin-type O-glycosylation sites.
36014368	4	54	gly	N-glycosylation	644:658	arg2	22 predicted N-glycosylation consensus sites			22 predicted N-glycosylation consensus sites						sites	The SARS-CoV-2 S protein is heavily glycosylated with 22 predicted N-glycosylation consensus sites as well as numerous mucin-type O-glycosylation sites.
36252443	3	34	gly	glycoproteins	499:511	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			To date, the mechanisms, and contributions of glycans to the biological activities of glycoproteins remain to be elucidated.
36189205	2	19	gly	N-glycan	176:183	arg1	the Fc region			the Fc region	the Fc region		Site			region	The conserved N-glycan in the Fc region of IgG1 impacts interaction of IgG with FcγRs and the resulting effector functions, which has led to the design of antibody therapeutics with greatly improved antibody-dependent cell cytotoxicity (ADCC) activities.
36598201	7	109	gly	N-glycosylation	1101:1115	arg1	NSP4	NSP4				OGER		NSP4	Q14B24		Specifically, replications of glycosylation-defective virus in MA104 and HT29 cells were 10- and 100,000-fold lower, respectively, than that of the wild-type, suggesting that N-glycosylation of NSP4 plays a critical role in RV replication.
35616904	7	33	gly	detected	1020:1027	arg2	galectin-3-binding protein AND heavy fucosylation	galectin-3-binding protein			heavy fucosylation	PUBTATOR		galectin-3-binding protein	3959		As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	33	gly	detected	1020:1027	arg1	seminal plasma glycoproteins AND heavy fucosylation	seminal plasma glycoproteins			heavy fucosylation	Fterm		glycoproteins			As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	33	gly	detected	1020:1027	arg1	clusterin AND heavy fucosylation	clusterin			heavy fucosylation	OGER		clusterin	P10909		As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	70	gly	fucosylation	966:977	arg1	galectin-3-binding protein	galectin-3-binding protein				PUBTATOR		galectin-3-binding protein	3959		As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	70	gly	fucosylation	966:977	arg1	seminal plasma glycoproteins	seminal plasma glycoproteins				Fterm		glycoproteins			As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	70	gly	fucosylation	966:977	arg1	clusterin	clusterin				OGER		clusterin	P10909		As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	71	gly	glycoproteins	1047:1059	arg1	galectin-3-binding protein	galectin-3-binding protein				PUBTATOR		galectin-3-binding protein	3959		As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	71	gly	glycoproteins	1047:1059	arg1	seminal plasma glycoproteins	seminal plasma glycoproteins				Fterm		glycoproteins			As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	71	gly	glycoproteins	1047:1059	arg1	clusterin	clusterin				OGER		clusterin	P10909		As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
36813234	5	1	gly	P-glycoprotein	826:839	arg1	P-glycoprotein	P-glycoprotein				Fterm		P-glycoprotein			By contrast, the expression levels of both P-glycoprotein (P-gp) and its intracellular key regulator, NF-κB signaling, are significantly increased in K562/ADR cells.
37040463	0	29	gly	Azido-Sialoglycoproteins	79:102	arg1	Clickable Azido-Sialoglycoproteins	Clickable Azido-Sialoglycoproteins				Fterm		Azido-Sialoglycoproteins			GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building Clickable Azido-Sialoglycoproteins.
32485989	2	12	gly	glycoprotein	264:275	arg1	This 34 kDa glycoprotein	This 34 kDa glycoprotein				Fterm		glycoprotein			This 34 kDa glycoprotein, termed VaaSP-VX, possesses five kDa N-linked carbohydrates.
32485989	2	57	gly	possesses	295:303	arg1	This 34 kDa glycoprotein AND five kDa N-linked carbohydrates	This 34 kDa glycoprotein			five kDa N-linked carbohydrates	Fterm		glycoprotein			This 34 kDa glycoprotein, termed VaaSP-VX, possesses five kDa N-linked carbohydrates.
37238633	6	47	gly	core-fucosylated	1039:1054	arg1	agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans				agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans						We observed an increase in agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans (padj = 1.00 × 10-4, 2.41 × 10-25, 1.51 × 10-21 and 3.38 × 10-30, respectively) and a decrease in digalactosylated, mono- and di-sialylated N-glycans (padj = 4.93 × 10-12, 7.61 × 10-9 and 1.09 × 10-28, respectively).
37238633	6	49	gly	asialylated	1023:1033	arg1	agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans				agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans						We observed an increase in agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans (padj = 1.00 × 10-4, 2.41 × 10-25, 1.51 × 10-21 and 3.38 × 10-30, respectively) and a decrease in digalactosylated, mono- and di-sialylated N-glycans (padj = 4.93 × 10-12, 7.61 × 10-9 and 1.09 × 10-28, respectively).
37238633	6	53	gly	di-sialylated	1192:1204	arg1	digalactosylated, mono- and di-sialylated N-glycans				digalactosylated, mono- and di-sialylated N-glycans						We observed an increase in agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans (padj = 1.00 × 10-4, 2.41 × 10-25, 1.51 × 10-21 and 3.38 × 10-30, respectively) and a decrease in digalactosylated, mono- and di-sialylated N-glycans (padj = 4.93 × 10-12, 7.61 × 10-9 and 1.09 × 10-28, respectively).
36660462	3	24	gly	glycopeptide	506:517	arg2	intact glycopeptide			intact glycopeptide						glycopeptide	Here, tandem mass tag labeling combined with LC-MS/MS was employed to determine the global expression of all proteins and intact glycopeptide in placentas from three healthy pregnant women, three patients with early-onset severe PE, and three patients with late-onset severe PE.
35504880	3	29	gly	glycopeptide	454:465	arg2	a glycopeptide			a glycopeptide						glycopeptide	Through biophysical and cellular studies, including X-ray crystallography of C1GalT1 complexed to a glycopeptide, we report that C1GalT1 is an obligate GT-A fold dimer that follows a SN2 mechanism.
35504880	3	48	gly	GT-A	506:509	arg1	C1GalT1	GT-A			C1GalT1	PUBTATOR		GT-A	3674		Through biophysical and cellular studies, including X-ray crystallography of C1GalT1 complexed to a glycopeptide, we report that C1GalT1 is an obligate GT-A fold dimer that follows a SN2 mechanism.
36036581	3	16	gly	N-glycosylation	380:394	arg1	the S protein	the S protein				PUBTATOR		S protein	7448		Here, we further provide structural-clear N-glycosylation of the S protein at a site-specific level by using our recently developed structural- and site-specific N-glycoproteomics sequencing algorithm, StrucGP.
36585837	3	33	gly	attachment	324:333	arg2	asparagine AND glycans			asparagine	glycans					asparagine	Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-link glycosylation.
36585837	3	48	gly	attachment	277:286	arg2	proteins AND sugar moieties	proteins			sugar moieties	Fterm		proteins			Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-link glycosylation.
37071468	2	14	gly	sialylated	241:250	arg1	the optimal Fc-glycan				the optimal Fc-glycan						The α-2,6 sialylated biantennary complex type glycan was identified as the optimal Fc-glycan with significant enhancement in antibody effector functions, including binding to different Fc receptors and ADCC.
37071468	2	14	gly	sialylated	241:250	arg1	The α-2,6 sialylated biantennary complex type glycan				The α-2,6 sialylated biantennary complex type glycan						The α-2,6 sialylated biantennary complex type glycan was identified as the optimal Fc-glycan with significant enhancement in antibody effector functions, including binding to different Fc receptors and ADCC.
32796070	2	55	gly	glycoprotein	336:347	arg1	the viral Env glycoprotein gp120	the viral Env glycoprotein gp120				PUBTATOR		Env glycoprotein	100616444		HIV-1 susceptibility to SERINC5 is determined by sequences in the viral Env glycoprotein gp120, and the antiviral effect of SERINC5 is counteracted by the viral accessory protein Nef.
31657490	8	4	gly	glycopeptide	1460:1471	arg2	glycopeptide analysis			glycopeptide analysis						glycopeptide	CONCLUSIONS The experimental results showed that GO@PDA@MIL-125-NH2 @Au@L-Cys exhibited excellent performance on glycopeptide analysis.
36881660	3	33	gly	glycans	478:484	arg1	the variable region			the variable region	the variable region		Site			region	In this technical note, we propose a method for facile characterization of glycans in the variable region of antibodies using sequential enzymatic digests with Endoglycosidase-S2 and Rapid™ Peptide-N-Glycosidase-F followed by labeling with a fluorescent dye carrying an NHS-carbamate moiety.
35960654	3	80	gly	glycopeptides	573:585	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Relatively large amounts of starting materials are needed for the enrichment, which makes the identification and quantification of intact glycopeptides from samples with limited quantity more challenging.
37129482	3	6	gly	glycoproteins	490:502	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycogenes, glycoproteins, lectins, pathways, and disease information related to glycans are accessible from GlyCosmos.
35366731	5	33	gly	N-glycosylation	537:551	arg2	only one N-glycosylation site			only one N-glycosylation site						site	RhGlu16B has only one N-glycosylation site in its Ala55-Gly64 loop.
35622127	0	91	gly	N-glycosylation	50:64	arg1	IgG	IgG				Cterm		IgG			Children at onset of type 1 diabetes show altered N-glycosylation of plasma proteins and IgG.
35622127	0	91	gly	N-glycosylation	50:64	arg1	plasma proteins	plasma proteins				Fterm		proteins			Children at onset of type 1 diabetes show altered N-glycosylation of plasma proteins and IgG.
36385669	4	5	gly	contained	713:721	arg1	both isoforms AND high mannose glycans	both isoforms			high mannose glycans	Fterm		isoforms			Mass spectrometry data indicated that both isoforms contained high mannose glycans being mannose 6 the most abundant form.
37266972	5	22	gly	deglycosylated	722:735	arg1	deglycosylated proteins	deglycosylated proteins				Fterm		proteins			Here, we report on the generation of BY-2 cell lines producing deglycosylated proteins.
31657490	6	85	gly	glycopeptides	1138:1150	arg2	glycopeptides			glycopeptides						glycopeptides	RESULTS In the actual enrichment process, GO@PDA@MIL-125-NH2 @Au@L-Cys nanomaterials exhibited high selectivity (1:1000), outstanding sensitivity (0.5 fmol), and excellent repeatability for the enrichment of glycopeptides.
35077806	6	31	gly	found	966:970	arg2	vertebrate proteins AND various N- and O-glycan structures	vertebrate proteins			various N- and O-glycan structures	Fterm		proteins			Our analyses allowed the annotation of various N- and O-glycan structures commonly found in vertebrate proteins.
34611874	7	48	gly	terminus	1230:1237	arg1	both glycan reducing terminus fluorophore labeling				both glycan reducing terminus fluorophore labeling						In this chapter, we describe an analytical method for the UHPLC separation of plasma N-glycans which utilizes both glycan reducing terminus fluorophore labeling, to ensure stoichiometric analysis of relative glycan abundance, and online mass spectrometry for glycan identification.
32820167	4	23	gly	O-glycosites	497:508	arg2	the identified O-glycosites			the identified O-glycosites						O-glycosites	Many of the identified O-glycosites are conserved and are predicted to serve roles in proprotein processing, receptor interaction, biodistribution and biostability.
36007953	1	19	gly	N-glycosylated	214:227	arg1	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein				Fterm		protein			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S protein) is highly N-glycosylated, and a "glycan shield" is formed to limit the access of other molecules; however, a small open area coincides with the interface to the host's receptor and also neutralising antibodies.
36007953	1	19	gly	N-glycosylated	214:227	arg1	S protein	S protein				OGER		S protein	Q15517		Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S protein) is highly N-glycosylated, and a "glycan shield" is formed to limit the access of other molecules; however, a small open area coincides with the interface to the host's receptor and also neutralising antibodies.
33609912	6	61	gly	deglycosylated	982:995	arg1	purified IgG1 and neutralizing IgG1	purified IgG1 and neutralizing IgG1				OGER		IgG1	P01857		The activity of purified IgG1 and neutralizing IgG1 deglycosylated by PNGase F enzyme were analyzed using the rapid fluorescent focus inhibition test.
35077806	10	51	gly	glycoproteins	1441:1453	arg1	O- linked glycoproteins	O- linked glycoproteins				Fterm		glycoproteins			Finally, secreted N- and O- linked glycoproteins were predicted from the transcriptomic data, indicating that proteases and protease inhibitors are putative sources of the glycans described herein.
34712242	1	6	gly	glycan	299:304	arg1	the Fc region			the Fc region	the Fc region		Site			region	Rhesus macaques are a common non-human primate model used in the evaluation of human monoclonal antibodies, molecules whose effector functions depend on a conserved N-linked glycan in the Fc region.
34689907	8	67	gly	glycoprotein	1610:1621	arg1	Human Immunoglobulin G	Human Immunoglobulin G				Cterm		Human Immunoglobulin G			The new approach was demonstrated to be applicable for the analysis of N-linked oligosaccharides released from a model glycoprotein (Human Immunoglobulin G) and applied to map N-glycans from human serum for congenital disorders of glycosylation (CDG) diagnosis.
34689907	8	67	gly	glycoprotein	1610:1621	arg1	a model glycoprotein	a model glycoprotein				Fterm		glycoprotein			The new approach was demonstrated to be applicable for the analysis of N-linked oligosaccharides released from a model glycoprotein (Human Immunoglobulin G) and applied to map N-glycans from human serum for congenital disorders of glycosylation (CDG) diagnosis.
36754227	3	14	gly	N-glycan	426:433	arg1	SV2C	SV2C			N-glycan	OGER		SV2C	Q496J9		Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
36754227	3	14	gly	N-glycan	426:433	arg1	the luminal domain			the luminal domain	the luminal domain		Site			domain	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
36754227	3	42	gly	position	586:593	arg1	559			559						position 559	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
36754227	3	77	gly	position	438:445	arg1	the N-glycan			position 480	the N-glycan					position 480	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
34957216	1	65	gly	glycoprotein	308:319	arg1	S	S				PUBTATOR		S	43740568		Severe Acute respiratory syndrome coronavirus (SARS-CoV-1) attaches to the host cell surface to initiate the interaction between the receptor-binding domain (RBD) of its spike glycoprotein (S) and the human Angiotensin-converting enzyme (hACE2) receptor.
34957216	1	65	gly	glycoprotein	308:319	arg1	its spike glycoprotein	its spike glycoprotein				Fterm		glycoprotein			Severe Acute respiratory syndrome coronavirus (SARS-CoV-1) attaches to the host cell surface to initiate the interaction between the receptor-binding domain (RBD) of its spike glycoprotein (S) and the human Angiotensin-converting enzyme (hACE2) receptor.
35943155	14	81	gly	fucosylated	2298:2308	arg1	These fucosylated glycans				These fucosylated glycans						These fucosylated glycans are involved in a number of mammalian physiological processes, including adhesion of pathogens and immune responses.
35017635	4	30	gly	fucosylated	801:811	arg1	O-glycan fucosylated structures				O-glycan fucosylated structures						These different glycan profiles, confirmed by glycomics, can be distinguished by the expression of O-glycan fucosylated structures, present only in epithelial cells and regulated by the expression of GALNT3.
36373229	1	58	gly	glycoproteins	144:156	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			O-Glycoproteases are an emerging class of enzymes that selectively digest glycoproteins at positions decorated with specific O-linked glycans.
31941975	7	32	gly	N-glycoproteins	1343:1357	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Finally, this model was applied to classify fucosylated N-glycoproteins from human plasma.
31941975	7	76	gly	fucosylated	1331:1341	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Finally, this model was applied to classify fucosylated N-glycoproteins from human plasma.
35887202	5	55	gly	O-glycopeptide	731:744	arg2	MUC1 O-glycopeptide			MUC1 O-glycopeptide						O-glycopeptide	The aim of this study is to elucidate the mode of interaction between MY.1E12 and MUC1 O-glycopeptide by NMR, molecular dynamics (MD) and docking simulations.
35065968	1	64	gly	linked	264:269	arg2	an N-methylated threonine AND GlcNAc			an N-methylated threonine	GlcNAc					threonine	The type A glycan modification found in human pathogen Clostridioides difficile consists of a monosaccharide (GlcNAc) that is linked to an N-methylated threonine through a phosphodiester bond.
35065968	1	64	gly	linked	264:269	arg2	an N-methylated threonine AND a monosaccharide			an N-methylated threonine	a monosaccharide					threonine	The type A glycan modification found in human pathogen Clostridioides difficile consists of a monosaccharide (GlcNAc) that is linked to an N-methylated threonine through a phosphodiester bond.
36985724	1	7	gly	glycoproteins	219:231	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Protein N-glycosylation is a common post-translational modification that plays significant roles on the structure, property, and function of glycoproteins.
37270132	1	16	gly	HEP-1	189:193	arg1	A low molecular weight polysaccharides	HEP			A low molecular weight polysaccharides	OGER		HEP	Q5SXM8		A low molecular weight polysaccharides of HEP-1, with molecular weights of 1.67 × 104 Da and composition of →6)-β-D-Glcp-(1→, →3)-β-D-Glcp-(1→, β-D-Glcp-(1→ and →3,6)-β-D-Glcp-(1→, was isolated and characterized from the fruiting body of Hericium erinaceus.
37270132	1	41	gly	composition	240:250	arg1	β-D-Glcp-			position	β-D-Glcp-					position	A low molecular weight polysaccharides of HEP-1, with molecular weights of 1.67 × 104 Da and composition of →6)-β-D-Glcp-(1→, →3)-β-D-Glcp-(1→, β-D-Glcp-(1→ and →3,6)-β-D-Glcp-(1→, was isolated and characterized from the fruiting body of Hericium erinaceus.
35304921	2	46	gly	integrin	342:349	arg1	the first comprehensive site-specific glycan map	integrin			the first comprehensive site-specific glycan map	Fterm		integrin			RESULTS Here, we have established the first comprehensive site-specific glycan map of α5 β1 integrin that was purified from a natural source, that is, rat liver.
32409323	2	85	gly	modified	345:352	arg3	Most proteins AND N-glycans	Most proteins			N-glycans	Fterm		proteins			Most proteins in the secretory pathway, such as matrix metalloproteinases, are modified with N-glycans and play important roles in the development of osteoarthritis (OA).
32409323	2	85	gly	modified	345:352	arg3	matrix metalloproteinases AND N-glycans	matrix metalloproteinases			N-glycans	Fterm		metalloproteinases			Most proteins in the secretory pathway, such as matrix metalloproteinases, are modified with N-glycans and play important roles in the development of osteoarthritis (OA).
33316265	2	33	gly	modification	334:345	arg3	eNOS AND O-GlcNAc modification	eNOS			O-GlcNAc modification	PUBTATOR		eNOS	24600		MAIN METHODS O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR).
33316265	2	33	gly	modification	334:345	arg3	endothelial nitric oxide synthase AND O-GlcNAc modification	endothelial nitric oxide synthase			O-GlcNAc modification	PUBTATOR		endothelial nitric oxide synthase	24600		MAIN METHODS O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR).
33316265	2	43	gly	synthase	375:382	arg1	MAIN METHODS O-GlcNAc levels	endothelial nitric oxide synthase			MAIN METHODS O-GlcNAc levels	PUBTATOR		endothelial nitric oxide synthase	24600		MAIN METHODS O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR).
33316265	2	43	gly	synthase	375:382	arg1	O-GlcNAc modification	endothelial nitric oxide synthase			O-GlcNAc modification	PUBTATOR		endothelial nitric oxide synthase	24600		MAIN METHODS O-GlcNAc levels and O-GlcNAc modification of endothelial nitric oxide synthase (eNOS) were determined in aorta (conductance vessel) and mesenteric arteries (resistance vessels) of non-pregnant (NP) and pregnant (P) Wistar rats and spontaneously hypertensive rats (SHR).
35335137	9	24	gly	structures	1084:1093	arg1	unilateral AMD	AMD			structures	OGER		AMD	P17707		Multivariate analysis suggested a significant decrease in the immunomodulatory bi-antennary glycan structures in unilateral AMD (adjusted odds ratio 0.43 (95% confidence interval 0.22-0.79)).
35651042	4	36	gly	TFP	601:603	arg1	constituent monosaccharide	TFP			constituent monosaccharide	OGER		TFP	Q9HCM9		The results revealed that the reducing sugar content, chemical composition, molecular weight, rheological property, constituent monosaccharide, and FT-IR spectrum of TFP were not altered after the in vitro simulated digestion, indicating that it was indigestible under different simulated digestion conditions.
35651042	4	36	gly	TFP	601:603	arg1	the reducing sugar content	TFP			the reducing sugar content	OGER		TFP	Q9HCM9		The results revealed that the reducing sugar content, chemical composition, molecular weight, rheological property, constituent monosaccharide, and FT-IR spectrum of TFP were not altered after the in vitro simulated digestion, indicating that it was indigestible under different simulated digestion conditions.
36740532	5	10	gly	glycopeptide	841:852	arg2	glycopeptide analysis			glycopeptide analysis						glycopeptide	METHODS GPIbα ectodomain that was recombinantly expressed or that was purified from human platelets was analyzed by Western blot, mass spectrometry glycomics, and mass spectrometry glycopeptide analysis to define glycosites and the structures of the attached glycans.
36740532	5	4	gly	glycosites	873:882	arg1	the attached glycans			glycosites	the attached glycans					glycosites	METHODS GPIbα ectodomain that was recombinantly expressed or that was purified from human platelets was analyzed by Western blot, mass spectrometry glycomics, and mass spectrometry glycopeptide analysis to define glycosites and the structures of the attached glycans.
32356523	1	31	gly	glycoproteins	388:400	arg1	a cargo receptor	a cargo receptor				Fterm		receptor			The transmembrane intracellular lectin ER-Golgi intermediate compartment protein 53 (ERGIC-53) and the soluble EF-hand multiple coagulation factor deficiency protein 2 (MCFD2) form a complex that functions as a cargo receptor, trafficking various glycoproteins between the endoplasmic reticulum (ER) and the Golgi apparatus.
32356523	1	31	gly	glycoproteins	388:400	arg1	trafficking various glycoproteins	trafficking various glycoproteins				Fterm		glycoproteins			The transmembrane intracellular lectin ER-Golgi intermediate compartment protein 53 (ERGIC-53) and the soluble EF-hand multiple coagulation factor deficiency protein 2 (MCFD2) form a complex that functions as a cargo receptor, trafficking various glycoproteins between the endoplasmic reticulum (ER) and the Golgi apparatus.
37357420	1	4	gly	fucosylated	174:184	arg1	a fucosylated cell-surface glycan				a fucosylated cell-surface glycan						Sialyl Lewis X (sLex ) antigen is a fucosylated cell-surface glycan that is normally involved in cell-cell interactions.
37357420	1	4	gly	fucosylated	174:184	arg1	Sialyl Lewis X (sLex ) antigen				Sialyl Lewis X (sLex ) antigen						Sialyl Lewis X (sLex ) antigen is a fucosylated cell-surface glycan that is normally involved in cell-cell interactions.
34695439	0	51	gly	glycosylation	47:59	arg1	TSP1	TSP1				PUBTATOR		TSP1	7057		Loss of the AMD-associated B3GLCT gene affects glycosylation of TSP1 without impairing secretion in retinal pigment epithelial cells.
34102146	3	44	gly	sialo-glycoprotein	627:644	arg1	the underlying sialo-glycoprotein	the underlying sialo-glycoprotein				Fterm		sialo-glycoprotein			To determine the underlying sialo-glycoprotein, various regions in adjacent tissue sections were subjected to microdigestion and manual glycoproteomic analysis.
37258966	3	26	gly	glycosylated	478:489	arg1	aberrantly glycosylated proteins	aberrantly glycosylated proteins				Fterm		proteins			They also play an integral role in the immune system, and aberrantly glycosylated proteins have wide ranging effects, including leading to diseases such as autoimmune conditions and cancer.
34939096	4	24	gly	mAbs	1122:1125	arg1	sialylation	IgG4 mAbs			sialylation	OGER		IgG4 mAbs	P01861		Scalability of the reactions was demonstrated for mAb amounts ranging from 1 mg to 1 g. Additionally, the reactions of β1,4-galactosyltransferase and α2,6-sialyltransferase were shown to work on column during affinity chromatography using Protein A or KappaSelect, the latter providing more efficient galactosylation and sialylation of IgG1 and IgG4 mAbs.
34939096	4	83	gly	sialylation	1093:1103	arg1	IgG1 and IgG4 mAbs	IgG1 and IgG4 mAbs				OGER		IgG4 mAbs	P01861		Scalability of the reactions was demonstrated for mAb amounts ranging from 1 mg to 1 g. Additionally, the reactions of β1,4-galactosyltransferase and α2,6-sialyltransferase were shown to work on column during affinity chromatography using Protein A or KappaSelect, the latter providing more efficient galactosylation and sialylation of IgG1 and IgG4 mAbs.
34878920	1	7	gly	glycoprotein	115:126	arg1	Env	Env				PUBTATOR		Env	64006		Glycans on envelope glycoprotein (Env) of the subgroup J avian leukosis virus (ALV-J) play an essential role in the virion integrity and infection process.
34878920	1	7	gly	glycoprotein	115:126	arg1	envelope glycoprotein	envelope glycoprotein				PUBTATOR		envelope glycoprotein	64006		Glycans on envelope glycoprotein (Env) of the subgroup J avian leukosis virus (ALV-J) play an essential role in the virion integrity and infection process.
34878920	1	85	gly	Glycans	95:101	arg1	Env	Env			Glycans	PUBTATOR		Env	64006		Glycans on envelope glycoprotein (Env) of the subgroup J avian leukosis virus (ALV-J) play an essential role in the virion integrity and infection process.
34878920	1	85	gly	Glycans	95:101	arg1	envelope glycoprotein	envelope glycoprotein			Glycans	PUBTATOR		envelope glycoprotein	64006		Glycans on envelope glycoprotein (Env) of the subgroup J avian leukosis virus (ALV-J) play an essential role in the virion integrity and infection process.
35616904	4	7	gly	N-glycosites	708:719	arg2	1019 N-glycosites			1019 N-glycosites						N-glycosites	A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
35616904	4	67	gly	glycopeptides	602:614	arg2	9567 unique glycopeptides			9567 unique glycopeptides						glycopeptides	A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
35616904	4	62	gly	glycoproteins	730:742	arg1	620 glycoproteins	glycoproteins			773 N-linked glycan structures	Fterm		glycoproteins			A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
35343965	4	6	gly	glycoproteins	519:531	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In pathogenic bacteria, glycoproteins have been involved in different stages of the infectious process, and glycan modifications can interfere with specific functions of glycoproteins.
35343965	4	85	gly	glycoproteins	665:677	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In pathogenic bacteria, glycoproteins have been involved in different stages of the infectious process, and glycan modifications can interfere with specific functions of glycoproteins.
32531122	0	40	gly	N-glycosylated	0:13	arg1	N-glycosylated IgG	N-glycosylated IgG				Cterm		IgG			N-glycosylated IgG in patients with kidney transplants increases calcium/calmodulin kinase IV in podocytes and causes injury.
36252735	2	39	gly	glycoproteins	232:244	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The glycans and glycoproteins are involved in cell-cell interactions that are of fundamental importance to the brain.
36606688	3	29	gly	contains	362:369	arg1	an extracellular superoxide dismutase AND a single N-glycan chain	an extracellular superoxide dismutase			a single N-glycan chain	Fterm		dismutase			SOD3 is an extracellular superoxide dismutase and contains a single N-glycan chain.
36606688	3	29	gly	contains	362:369	arg1	SOD3 AND a single N-glycan chain	SOD3			a single N-glycan chain	PUBTATOR		SOD3	6649		SOD3 is an extracellular superoxide dismutase and contains a single N-glycan chain.
33073321	5	48	gly	glycopeptides	1090:1102	arg2	glycopeptides			glycopeptides						glycopeptides	More excitingly, glycopeptides in urine of healthy people and patients with kidney cancer were successfully enriched and identified by the combined liquid chromatography-mass spectrometry/mass spectrometry technology (LC-MS/MS).
36437732	5	82	gly	released	799:806	arg2	serum glycoproteins AND N-glycans	serum glycoproteins			N-glycans	Fterm		glycoproteins			N-glycans were enzymatically released from serum glycoproteins, labeled via permethylation, and analyzed by MALDI-TOF mass spectrometry.
36437732	5	89	gly	glycoproteins	819:831	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			N-glycans were enzymatically released from serum glycoproteins, labeled via permethylation, and analyzed by MALDI-TOF mass spectrometry.
37162352	8	5	gly	O-glycosylated	1186:1199	arg1	Six proteins	Six proteins				Fterm		proteins			Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated.
35887202	6	51	gly	O-glycosylation	921:935	arg2	the O-glycosylation site			the O-glycosylation site						site	NMR titration using MUC1 O-glycopeptides suggests that the epitope is located within the O-linked glycan and near the O-glycosylation site.
35887202	6	53	gly	O-glycopeptides	828:842	arg2	MUC1 O-glycopeptides			MUC1 O-glycopeptides						O-glycopeptides	NMR titration using MUC1 O-glycopeptides suggests that the epitope is located within the O-linked glycan and near the O-glycosylation site.
36089065	5	81	gly	glycoproteins	896:908	arg1	These glycoproteins	These glycoproteins				Fterm		glycoproteins			These glycoproteins were then sequentially modified in vitro from high mannose to hybrid and on to biantennary, core-fucosylated, complex structures by a panel of N-glycosylation enzymes, and each reaction time course was quantified by LC-MS/MS.
36809652	2	32	gly	galectin-1	357:366	arg1	The O-glycan structures	galectin-1			The O-glycan structures	OGER		galectin-1	P09382		The O-glycan structures of apo(a) subunit of Lp(a) serve as strong ligands of galectin-1, an O-glycan binding pro-angiogenic lectin abundantly expressed in placental vascular tissues.
36809652	2	105	gly	subunit	313:319	arg1	The O-glycan structures	subunit			The O-glycan structures	Fterm		subunit			The O-glycan structures of apo(a) subunit of Lp(a) serve as strong ligands of galectin-1, an O-glycan binding pro-angiogenic lectin abundantly expressed in placental vascular tissues.
33493676	7	99	gly	glycopeptides	1204:1216	arg2	AGP glycopeptides			AGP glycopeptides						glycopeptides	After trypsinization, AGP glycopeptides are purified using a hydrophilic interaction chromatography-based solid-phase extraction and analyzed by reversed-phase-liquid chromatography-electrospray ionization-MS.
36329887	6	18	gly	Asn<sup>52</sup>	1184:1199	arg1	Asn<sup>52</sup> glycans			Asn	Asn<sup>52</sup> glycans					Asn	As the most active FSH21 preparations possessed more rapidly migrating α-subunit bands in Western blots, we hypothesized that Asn<sup>52</sup> glycans in these preparations were small enough to enable greater FSH21 receptor occupancy in the putative FSHR trimer model.
34778211	9	45	gly	glycosites	1516:1525	arg2	928 identified glycosites			928 identified glycosites						glycosites	Moreover, 28.62% in 442 identified glycoproteins and 26.69% in 928 identified glycosites were significantly changed in the FUT8KO CHO compared to wild-type CHO cells.
34778211	9	93	gly	glycoproteins	1473:1485	arg1	442 identified glycoproteins	442 identified glycoproteins				Fterm		glycoproteins			Moreover, 28.62% in 442 identified glycoproteins and 26.69% in 928 identified glycosites were significantly changed in the FUT8KO CHO compared to wild-type CHO cells.
36380917	6	14	gly	glycopeptides/glycoproteins	1335:1361	arg1	N-glycan			glycopeptides/glycoproteins	N-glycan					glycopeptides/glycoproteins	The information learned would facilitate future design of therapeutic glycopeptides/glycoproteins with N-glycan to achieve enhanced pharmacological properties.
33710874	9	14	gly	motifs	1679:1684	arg1	a model peptide			a model peptide						peptide	The plug-and-play trifunctional platform was exemplified by attaching clinically relevant motifs: biotin, fluorescein, a polyethylene glycol chain, and a model peptide.
34626388	3	56	gly	galactosamine	563:575	arg1	heparin	heparin			galactosamine	Fterm		heparin			Testing galactosamine in heparin has been added to the USP monograph after contaminated heparin event, but the general monosaccharide composition analysis has not been developed for GAG quality control purposes.
37398203	8	36	gly	-glycan	1416:1422	arg1	the Fab region			the Fab region	the Fab region		Site			region	Incorporation of enzymatic de- N -glycosylation into the peptide mapping routine allows the N -glycan in the Fab region of the antibody to be localized to the framework 3 region of the V H domain.
35219398	4	29	gly	glycosylation	675:687	arg1	dimer formation	dimer formation				Fterm		dimer			GFCC'C″ was identified as the dominant CCM1 solution dimer, but ambiguity regarding the effect of glycosylation on dimer formation calls its physiological relevance into question.
36912209	8	32	gly	position	1479:1486	arg1	the carbohydrate			position	the carbohydrate					position	We highlight examples of acetylesterases that are highly specific to one substrate and to the position of the acetyl group on the glycosyl residue of the carbohydrate, while other members that have more broad substrate specificity.
33515675	10	1	gly	NOTCH1	1410:1415	arg1	the O-GlcNAcylation	NOTCH1			the O-GlcNAcylation	PUBTATOR		NOTCH1	18128		We further clarified the molecular mechanisms by which EOGT and SHCBP1 enhance the O-GlcNAcylation of NOTCH1, Subsequently promoting the nuclear localization of the Notch intracellular domain (NICD) and inhibiting the transcription of E-cadherin and P21 in pancreatic cancer cells.
36252443	10	79	gly	glycoproteins	1674:1686	arg1	native glycoproteins	native glycoproteins				Fterm		glycoproteins			EndoBI-1 released a broader array of glycan structures compared to PNGase F from native glycoproteins.
34778211	7	108	gly	glycopeptides	1082:1094	arg2	The glycopeptides			The glycopeptides						glycopeptides	The glycopeptides were enriched by hydrophilic chromatography and fractionated 25 fractions by bRPLC followed by analysis using high-resolution liquid chromatography mass spectrometry (LC-MS).
37316505	5	42	gly	sialylated	815:824	arg1	sialylated mucin-type O-glycan with core 1-				sialylated mucin-type O-glycan with core 1-						The result indicates that T153, S157, S159, and T168 are modified by sialylated mucin-type O-glycan with core 1- and core 2-based structures.
36122299	3	30	gly	glycopeptides	373:385	arg2	glycopeptides			glycopeptides						glycopeptides	Despite the great progress, characterization of structural isomers of glycans and glycopeptides remains challenging.
36036581	2	42	gly	protein	329:335	arg1	site-specific glycan compositions	S protein			site-specific glycan compositions	PUBTATOR		S protein	7448		Previous studies have systematically analyzed site-specific glycan compositions as well as many important structural motifs of the S protein.
33493676	1	55	gly	glycoprotein	198:209	arg1	Alpha-1-acid glycoprotein	glycoprotein				Fterm		glycoprotein			Alpha-1-acid glycoprotein (AGP) is an acute phase glycoprotein in blood, which is primarily synthetized in the liver and whose biological role is not completely understood.
33493676	1	69	gly	glycoprotein	161:172	arg1	an acute phase glycoprotein	glycoprotein				Fterm		glycoprotein			Alpha-1-acid glycoprotein (AGP) is an acute phase glycoprotein in blood, which is primarily synthetized in the liver and whose biological role is not completely understood.
37292769	7	59	gly	glycopeptide	1165:1176	arg2	synthetic stable isotope-labelled glycopeptide standards			synthetic stable isotope-labelled glycopeptide standards						glycopeptide	Here, we investigated fragmentation specificity using synthetic stable isotope-labelled glycopeptide standards.
34878920	2	78	gly	glycosylation	313:325	arg2	the 13 predicted N-linked glycosylation sites			the 13 predicted N-linked glycosylation sites						sites	In this study, we found that, among the 13 predicted N-linked glycosylation sites (NGSs) in gp85 of Tibetan chicken strain TBC-J6, N17, and N193/N191 are pivotal for virus replication.
36903897	3	41	gly	glycoproteins	635:647	arg1	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins				Fterm		glycoproteins			Here, our work with Hyp-O-polysaccharides isolated from (Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins overexpressed in transgenic Arabidopsis suspension culture is consistent with the common structural features of AGPs isolated from tobacco.
36903897	3	59	gly	isolated	560:567	arg2	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins AND Hyp-O-polysaccharides	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins			Hyp-O-polysaccharides	Fterm		glycoproteins			Here, our work with Hyp-O-polysaccharides isolated from (Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins overexpressed in transgenic Arabidopsis suspension culture is consistent with the common structural features of AGPs isolated from tobacco.
34778211	11	38	gly	core-fucosylated	1842:1857	arg1	core-fucosylated glycans				core-fucosylated glycans						Furthermore, a decrease in fucosylation content was observed in FUT8KO cells, in which core-fucosylated glycans almost disappeared as an effect of FUT8 gene knockout.
35247653	5	32	gly	glycoprotein	877:888	arg1	the intact glycoprotein	the intact glycoprotein				Fterm		glycoprotein			At the intact glycoprotein level, NeuroEPO plus glycoforms were separated by capillary zone electrophoresis with ultraviolet detection (CE-UV), optimizing the composition and pH of the separation electrolyte.
36409896	6	41	gly	glycoforms	1188:1197	arg1	IgG Fc glycoforms	IgG Fc glycoforms				Cterm		IgG			Ultimately, we provide a strategy for the development of reagents to identify and manipulate IgG Fc glycoforms.
32417172	8	89	gly	glycosylated	1265:1276	arg1	The p53	The p53				OGER		p53	P04637		RESULTS The p53 was O-GalNAc glycosylated in cells.
33316265	1	9	gly	proteins	201:208	arg1	O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			AIM We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats.
33316265	1	9	gly	proteins	201:208	arg1	O-linked N-acetylglucosamine	proteins			O-linked N-acetylglucosamine	Fterm		proteins			AIM We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats.
33912181	3	15	gly	glycans	454:460	arg1	TLR4	TLR4			glycans	PUBTATOR		TLR4	21898		The lectins MIC1 and MIC4 interact with N-linked glycans on TLR2 and TLR4, activating NF-κB and producing IL-12, TNF-α, and IL-6.
33912181	3	15	gly	glycans	454:460	arg1	TLR2	TLR2			glycans	PUBTATOR		TLR2	24088		The lectins MIC1 and MIC4 interact with N-linked glycans on TLR2 and TLR4, activating NF-κB and producing IL-12, TNF-α, and IL-6.
32273875	0	65	gly	Removal	0:6	arg3	Mannose-Ending Glycan AND Asn2118			Asn2118	Mannose-Ending Glycan			FVIII	14069	Asn2118	Removal of Mannose-Ending Glycan at Asn2118 Abrogates FVIII Presentation by Human Monocyte-Derived Dendritic Cells.
35848837	8	55	gly	Asn-glycans	1167:1177	arg1	Asn-glycans and free glycans			Asn	Asn-glycans and free glycans					Asn	The resulted Asn-glycans and free glycans were then purified using porous graphitic carbon cartridges, permethylated, and analyzed by mass spectrometry.
36737995	2	88	gly	glycosylation	343:355	arg1	free milk oligosaccharides				free milk oligosaccharides						However glycosylation of milk components other than free milk oligosaccharides are underinvestigated.
36192063	6	10	gly	N-glycoproteins	1380:1394	arg1	254 intact N-glycoproteins	254 intact N-glycoproteins				Fterm		N-glycoproteins			In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36192063	6	100	gly	N-glycopeptides	1256:1270	arg2	6384 intact N-glycopeptides			6384 intact N-glycopeptides						N-glycopeptides	In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36192063	6	96	gly	N-glycopeptides	1348:1362	arg1	254 intact N-glycoproteins	N-glycoproteins		N-glycopeptides		Fterm		N-glycoproteins		N-glycopeptides	In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36136114	8	19	gly	glycosylation	1303:1315	arg1	a fusion protein	a fusion protein				Fterm		protein			To overcome this drawback, we used nanoHILIC-MS to characterize the complex glycosylation of UTI-Fc, a fusion protein that greatly increases the half-life of ulinastatin.
32555531	2	37	gly	glycoprotein	483:494	arg1	glomalin-related soil protein	glomalin-related soil protein				Fterm		protein			In a field experiment, the temporal and spatial dynamics of AM fungi, glomalin-related soil protein (GRSP) - which is described as a N-linked glycoprotein and the putative gene product of AM fungi, SOC, and soil aggregates were investigated in halophyte Kosteletzkya virginica rhizosphere soil of coastal saline areas of North Jiangsu, China.
32555531	2	37	gly	glycoprotein	483:494	arg1	a N-linked glycoprotein	a N-linked glycoprotein				Fterm		glycoprotein			In a field experiment, the temporal and spatial dynamics of AM fungi, glomalin-related soil protein (GRSP) - which is described as a N-linked glycoprotein and the putative gene product of AM fungi, SOC, and soil aggregates were investigated in halophyte Kosteletzkya virginica rhizosphere soil of coastal saline areas of North Jiangsu, China.
33206215	2	27	gly	proteoglycans	557:569	arg1	lysines			lysines	lysines		AminoAcid			prolines and lysines	The extracellular matrix (ECM) consists of (1) fibrous proteins, post-translationally modified (PTM) via N- and O-linked glycosylation, as well as hydroxylation on prolines and lysines, and (2) glycosaminoglycan-decorated proteoglycans.
33206215	2	27	gly	proteoglycans	557:569	arg1	prolines			prolines	prolines		AminoAcid			prolines and lysines	The extracellular matrix (ECM) consists of (1) fibrous proteins, post-translationally modified (PTM) via N- and O-linked glycosylation, as well as hydroxylation on prolines and lysines, and (2) glycosaminoglycan-decorated proteoglycans.
36089065	6	44	gly	sites	1233:1237	arg1	the same protein	protein			sites	Fterm		protein			Substantial differences in rates of in vitro enzymatic modification were observed between glycan sites on the same protein, and differences in modification rates varied depending on the glycoenzyme being evaluated.
35995381	8	10	gly	glycopeptide	1267:1278	arg2	the glycopeptide level			the glycopeptide level						glycopeptide	In addition, sulfated glycoform structures were detected at the glycopeptide level in glycosylation sites containing complex and hybrid type glycans.
35995381	8	77	gly	glycosylation	1289:1301	arg2	glycosylation sites			glycosylation sites						sites	In addition, sulfated glycoform structures were detected at the glycopeptide level in glycosylation sites containing complex and hybrid type glycans.
35995381	8	68	gly	containing	1309:1318	arg1	glycosylation sites AND complex and hybrid type glycans			glycosylation sites	complex and hybrid type glycans					sites	In addition, sulfated glycoform structures were detected at the glycopeptide level in glycosylation sites containing complex and hybrid type glycans.
35393560	6	23	gly	glycoprotein	961:972	arg1	an impactful glycoprotein class	an impactful glycoprotein class				Fterm		glycoprotein			In this study, we quantitatively evaluate more than 30 enzymes for glycoengineering immobilized immunoglobulin G, an impactful glycoprotein class in the pharmaceutical field.
36096000	0	40	gly	Glycosylation	0:12	arg1	egg white protein	egg white protein				Fterm		protein			Glycosylation of egg white protein with maltodextrin in the dry state: Changes in structural and gel properties.
36252443	4	18	gly	deglycosylated	588:601	arg1	the deglycosylated protein	the deglycosylated protein				Fterm		protein			Only by testing both the released glycans and the deglycosylated protein in their native (i.e., non-denatured) form, can the individual contribution to the biological activity of glycoproteins be elucidated.
36252443	4	20	gly	glycoproteins	717:729	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Only by testing both the released glycans and the deglycosylated protein in their native (i.e., non-denatured) form, can the individual contribution to the biological activity of glycoproteins be elucidated.
37354227	3	11	gly	N-glycosylation	522:536	arg2	22 potential sites			22 potential sites						sites	The SARS-CoV-2 spike protein (S) is a prime example, having 22 potential sites of N-glycosylation per protein protomer, as predicted from the primary sequence.
36373229	4	4	gly	O-glycopeptides	752:766	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	One challenge of defining O-glycoprotease specificity restraints is the need to characterize O-glycopeptides with site-specific analysis of O-glycosites.
36373229	4	14	gly	O-glycosites	799:810	arg2	O-glycosites			O-glycosites						O-glycosites	One challenge of defining O-glycoprotease specificity restraints is the need to characterize O-glycopeptides with site-specific analysis of O-glycosites.
37079606	5	7	gly	afucosylated	1114:1125	arg1	IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan				IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan						RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	53	gly	fucosylated	1081:1091	arg1	IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan				IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan						RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	81	gly	trisialylated	1167:1179	arg1	IgA trisialylated triantennary (A3G3S3) glycan				IgA trisialylated triantennary (A3G3S3) glycan						RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	100	gly	protein	987:993	arg1	core fucosylation	protein			core fucosylation	Fterm		protein			RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	100	gly	protein	987:993	arg1	sialylation	protein			sialylation	Fterm		protein			RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
35211008	14	131	gly	α-1,3-mannosyl-glycoprotein	2613:2639	arg1	α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase A	α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase A				Fterm		α-1,3-mannosyl-glycoprotein	12044		Conclusion: Our results show that stachytine hydrochloride inhibits the synthesis of α-1,6-fucosylation on the N-terminal sugar chain by reducing α-1,6-fucosyltransferase (FUT8) and α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase A (MGAT4a), upregulating the N-glycosylation level on β1 adrenergic receptors, and maintaining cAMP/PKA signal pathway activation.
37186866	4	48	gly	domains	961:967	arg1	O-mannosylation			domains	O-mannosylation					domains	We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knockout in cells results in receptor maturation defects and abnormal growth of 3D cell models.
37186866	4	63	gly	O-mannosylation	938:952	arg1	IPT domains			IPT domains						domains	We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knockout in cells results in receptor maturation defects and abnormal growth of 3D cell models.
33946304	5	38	gly	glycoprotein	941:952	arg1	glycoprotein N-linked oligosaccharide processing				glycoprotein N-linked oligosaccharide processing						The mechanism resides in the inhibitory activity toward α-glucosidases that are involved in the early stages of glycoprotein N-linked oligosaccharide processing in the endoplasmic reticulum, leading to a marked decrease of the viral Spike protein.
32417172	0	55	gly	present	47:53	arg2	the tumor suppressor p53 AND O-linked N-acetylgalactosamine modification	tumor suppressor p53			O-linked N-acetylgalactosamine modification	OGER		tumor suppressor p53	P04637		O-linked N-acetylgalactosamine modification is present on the tumor suppressor p53.
36399934	8	80	gly	aglycone	1252:1259	arg1	Thr193			Thr193						Thr193	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	81	gly	glycone	1231:1237	arg1	binding sites			binding sites						sites	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	linker residues			linker residues						residues	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	Phe333			Leu192, Ile251, and Phe333						Leu192, Ile251, and Phe333	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	Leu192			Leu192, Ile251, and Phe333						Leu192, Ile251, and Phe333	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	Leu192			Leu192, Ile251, and Phe333						Leu192, Ile251, and Phe333	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
33006605	0	13	gly	glycoprotein	59:70	arg1	glycoprotein stability	glycoprotein stability				Fterm		glycoprotein			Systemic effects of missense mutations on SARS-CoV-2 spike glycoprotein stability and receptor-binding affinity.
31901900	3	36	gly	PD-L1	724:728	arg1	N-linked glycan decoration	PD-L1			N-linked glycan decoration	PUBTATOR		PD-L1	29126		Using biochemical assays, computer-aided docking/molecular dynamics simulations, and fluorescence microscopy, we found that RSV can operate as a direct inhibitor of glyco-PD-L1-processing enzymes (α-glucosidase/α-mannosidase) that modulate N-linked glycan decoration of PD-L1, thereby promoting the endoplasmic reticulum retention of a mannose-rich, abnormally glycosylated form of PD-L1.
33552045	10	27	gly	glycosylation	1695:1707	arg1	viral proteins	viral proteins				Fterm		proteins			Overall, this perspective is aimed to support future research on T cells recognizing glycosylated epitopes in order to expand our understanding on how glycosylation of viral proteins could alter host T cell immunity against viral infections.
33552045	10	76	gly	glycosylated	1629:1640	arg1	glycosylated epitopes			glycosylated epitopes						epitopes	Overall, this perspective is aimed to support future research on T cells recognizing glycosylated epitopes in order to expand our understanding on how glycosylation of viral proteins could alter host T cell immunity against viral infections.
36399685	6	6	gly	glycoprotein	1092:1103	arg1	an arrested glycoprotein	an arrested glycoprotein				Fterm		glycoprotein			Moreover, the observation that encoding mRNAs remained stably attached to ribosomes provides evidence of a genotype-glycophenotype link between an arrested glycoprotein and its RNA message.
36289103	7	99	gly	sialylated	977:986	arg1	sialylated N-glycans				sialylated N-glycans						The levels of sialylated N-glycans showed considerable cell line-dependent differences ranging from 24 to 35%.
32357974	10	2	gly	glycoprotein	2061:2072	arg1	glycoprotein antigens	glycoprotein antigens				Fterm		glycoprotein			The information herein will help train deamidation prediction models for HLA-peptide repertoires and aid in the design of novel T cell therapeutic targets derived from glycoprotein antigens.
32306345	3	30	gly	glycoproteins	391:403	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The microneme protein 4 (MIC4) has the property to recognize galactosides residues linked to glycoproteins on the host cell surface.
37146275	6	34	gly	glycosylation	1422:1434	arg2	the glycosylation site			the glycosylation site						site	A total of 677 quantifiable intact N-glycopeptides were identified from the serum of patients with cervical cancer, and the glycosylation site and glycan structure were analyzed in depth, indicating that this novel material can show a broad practical application in cervical cancer diagnosis.
37146275	6	35	gly	N-glycopeptides	1333:1347	arg2	677 quantifiable intact N-glycopeptides			677 quantifiable intact N-glycopeptides						N-glycopeptides	A total of 677 quantifiable intact N-glycopeptides were identified from the serum of patients with cervical cancer, and the glycosylation site and glycan structure were analyzed in depth, indicating that this novel material can show a broad practical application in cervical cancer diagnosis.
37292769	3	44	gly	glycoproteins	534:546	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The ability to distinguish between these complex glycan structures poses a significant challenge, hindering our ability to accurately measure and understand the role of glycoproteins in biological systems.
35960654	8	27	gly	glycopeptides	1614:1626	arg2	glycopeptides			glycopeptides						glycopeptides	Moreover, weighted gene coexpression network analysis (WGCNA) showed nine modules of glycopeptides, two of which were associated with the AD phenotype.
36053804	3	49	gly	enzyme	547:552	arg1	high-mannose glycan moieties	enzyme			high-mannose glycan moieties	Fterm		enzyme			Previously, RNAi-mediated silencing of Mannosidase-Ia (ManIa), a key enzyme in the transition from high-mannose glycan moieties to paucimannose N-glycans, was shown to disrupt the transition from larva to pupa and the metamorphosis into adult beetles.
36053804	3	69	gly	moieties	597:604	arg1	a key enzyme	enzyme			moieties	Fterm		enzyme			Previously, RNAi-mediated silencing of Mannosidase-Ia (ManIa), a key enzyme in the transition from high-mannose glycan moieties to paucimannose N-glycans, was shown to disrupt the transition from larva to pupa and the metamorphosis into adult beetles.
35320529	6	10	gly	structures	988:997	arg1	CD16a	CD16a			structures	PUBTATOR		CD16a	2214		Donor-dependent variability in N-glycan structures on CD16a isolated from primary NK cells of healthy human donors was recently reported.
32187935	16	0	gly	glycosylation	1916:1928	arg2	glycosylation sites			glycosylation sites						sites	A greater diversification of amino acid substitutions, and some deletion and insertion of glycosylation sites embodied the polymorphism of G protein as main protective antigen.
35193013	2	94	gly	glycoproteins	457:469	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The acidic sugars are attached to the end of glycoproteins, glycolipids or gangliosides.
37294165	7	44	gly	glycoprotein	1359:1370	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Because the two peptides used for this synthesis were prepared by a fast-flow SPPS, the total synthetic time of glycoprotein was considerably shortened.
34274480	12	44	gly	glycosylation	1553:1565	arg2	three possible N-linked glycosylation sites			three possible N-linked glycosylation sites						sites	The amino terminal ends of both the CBLN1 and CBLN3 proteins contain three possible N-linked glycosylation sites.
35304921	14	13	gly	glycosylation	1973:1985	arg2	the specific sites			the specific sites						sites	Yet, detailed knowledge of its glycosylation, notably the specific sites of glycosylation, is lacking.
37121976	4	8	gly	N-glycosylation	788:802	arg1	LRG1	structure of LRG1		sites		OGER		structure of LRG1	P02750	sites	Here, we determined the crystal structure of LRG1, identifying the horseshoe-like solenoid structure of LRG1 and its four N-glycosylation sites.
34379775	5	22	gly	fucosylated	1078:1088	arg1	fucosylated glycans				fucosylated glycans						We identified 124 O-glycans, from which 51 were the major glycans with core 2 and fucosylated glycans as the most common structures.
36422041	2	85	gly	modified	493:500	arg3	glycoproteins AND both N-linked and O-linked glycans	glycoproteins			both N-linked and O-linked glycans	Fterm		glycoproteins			Like other fungal pathogens, the Sporothrix cell wall is composed of structural polysaccharides and glycoproteins that are covalently modified with both N-linked and O-linked glycans.
36422041	2	92	gly	glycoproteins	459:471	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Like other fungal pathogens, the Sporothrix cell wall is composed of structural polysaccharides and glycoproteins that are covalently modified with both N-linked and O-linked glycans.
33176830	7	32	gly	N-glycosylation	1340:1354	arg2	non-canonical N-glycosylation motifs			non-canonical N-glycosylation motifs						motifs	It is also the first study to report the functional importance of non-canonical N-glycosylation motifs in an ion channel.
34695439	7	23	gly	present	1099:1105	arg1	WT TSP1 AND C-mannosylation	TSP1			C-mannosylation	PUBTATOR		TSP1	7057		C-mannosylation was variably present on WT TSP1 and increased on TSR domains 1 and 3 in KO cells.
36924942	4	84	gly	3-deoxy-β-d-manno-oct-2-ulosonic	593:624	arg1	β-Kdo	oct-2			β-Kdo	OGER		oct-2	P09086		Two separate GT modules in KpsC transfer 3-deoxy-β-d-manno-oct-2-ulosonic acid (β-Kdo) from cytidine-5'-monophospho-β-Kdo donor, to a glycolipid acceptor.
33073321	4	43	gly	glycopeptides	1036:1048	arg2	glycopeptides			glycopeptides						glycopeptides	Benefitting from the merits of Zr-MOF and glucose-6-phosphate, the as-designed composite exhibits good selectivity (the mass ratio of HRP digests to BSA digests was up to1:200) and low limit of detection (0.1 fmol μL-1) towards the recognition of glycopeptides from standard samples.
34939126	1	42	gly	glycoprotein	145:156	arg1	new glycoprotein variants	new glycoprotein variants				Fterm		glycoprotein			Glycoengineering ultimately allows control over glycosylation patterns to generate new glycoprotein variants with desired properties.
34650217	9	24	gly	KAT5	1451:1454	arg1	O-GlcNAcylation	KAT5			O-GlcNAcylation	OGER		KAT5	Q8CHK4		Collectively, our findings demonstrate that PCK1 depletion increases O-GlcNAcylation of KAT5, epigenetically induces TWIST1 expression and promotes HCC metastasis, and link metabolic enzyme, post-translational modification (PTM) with epigenetic regulation.
35092134	9	49	gly	domain	1404:1409	arg1	the N-glycans			domain	the N-glycans					domain	The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling.
35092134	9	54	gly	domain	1303:1308	arg1	the N-glycans			domain	the N-glycans					domain	The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling.
35092134	9	69	gly	region	1377:1382	arg1	the N-glycans				the N-glycans						The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling.
33244686	1	31	gly	glycosylation	306:318	arg2	multiple glycosylation site(s)			multiple glycosylation site(s)						site	Many biotherapeutics such as monoclonal antibodies (mAb) and Fc-domain fusion proteins contain heterogeneous glycan contents at one or multiple glycosylation site(s).
33244686	1	74	gly	contain	249:255	arg1	Fc-domain fusion proteins AND heterogeneous glycan contents	Fc-domain fusion proteins			heterogeneous glycan contents	Fterm		proteins			Many biotherapeutics such as monoclonal antibodies (mAb) and Fc-domain fusion proteins contain heterogeneous glycan contents at one or multiple glycosylation site(s).
34611874	4	7	gly	glycosylated	633:644	arg1	the proteins	the proteins				Fterm		proteins			Many of the proteins that constitute plasma are glycosylated with both N- and O-type glycans.
32187935	13	79	gly	glycosylation	1588:1600	arg2	glycosylation sites			glycosylation sites						sites	The HVR2 of the G protein varied in using stop condon, amino acid substitutions, glycosylation sites.
35022995	2	19	gly	residues	405:412	arg1	173-210			173-210						residues 173-210	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
35022995	2	40	gly	N-glycosylation	337:351	arg2	position 143			position 143						position 143	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
35022995	2	40	gly	N-glycosylation	337:351	arg2	one potential N-glycosylation site			one potential N-glycosylation site						site	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
36838558	2	18	gly	glycans	323:329	arg1	Gc proteins	proteins			glycans	Fterm		proteins			O-linked glycans in Gc proteins from human plasma mainly consist of trisaccharides.
37037133	6	7	gly	glycoproteins	1107:1119	arg1	donkey and human MFGM glycoproteins	donkey and human MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
37037133	6	23	gly	N-glycosylation	1066:1080	arg1	donkey and human MFGM glycoproteins	donkey and human MFGM glycoproteins				OGER		MFGM glycoproteins	Q08431		The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
37037133	6	41	gly	N-glycoproteins	1211:1225	arg1	human MFGM N-glycoproteins	human MFGM N-glycoproteins				OGER		MFGM N-glycoproteins	Q08431		The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
37037133	6	63	gly	N-glycoproteins	1009:1023	arg1	human MFGM N-glycoproteins	human MFGM N-glycoproteins				OGER		MFGM N-glycoproteins	Q08431		The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
36892535	5	27	gly	glycosylated	764:775	arg1	up to 6 differently glycosylated MUC1 peptides			up to 6 differently glycosylated MUC1 peptides						peptides	Cell adhesion experiments with MCF-7 cancer cells on surfaces carrying up to 6 differently glycosylated MUC1 peptides demonstrated that different glycans significantly impact adhesion.
36536394	3	35	gly	β-1,4-glycosylation	569:587	arg1	glucose				glucose						Bottom-up production of cello-oligosaccharides (COS) involves multistep iterative β-1,4-glycosylation of glucose from sucrose catalyzed by sucrose phosphorylase from Bifidobacterium adeloscentis (BaScP), cellobiose phosphorylase from Cellulomonas uda (CuCbP) and cellodextrin phosphorylase from Clostridium cellulosi (CcCdP).
35495330	2	70	gly	dimannosylated	417:430	arg1	Chemically synthesized dimannosylated HA peptides			Chemically synthesized dimannosylated HA peptides						peptides	Chemically synthesized dimannosylated HA peptides bound domain-swapped and dimeric CV-N with either four disulfide-bonds (Cys-Cys), or three Cys-Cys bonds and an intact fold of the high-affinity binding site at an equilibrium dissociation constant K D of 10 μM.
37341462	2	23	gly	N-glycopeptide	496:509	arg2	N-glycopeptide identification			N-glycopeptide identification						N-glycopeptide	The Y-ion pattern, a series of Y-ions with known mass gaps derived from the penta-saccharide core structure of N-linked glycosylation, is exploited to facilitate N-glycopeptide identification from their spectra.
36652139	1	69	gly	glycoprotein	154:165	arg1	a glycoprotein hormone	a glycoprotein hormone				Fterm		glycoprotein			Equine chorionic gonadotropin (eCG) is a glycoprotein hormone widely used in timed artificial ovulation (TAI) and superovulation protocols to improve the reproductive performance in livestock.
35647713	5	53	gly	glycoprotein	975:986	arg1	a viral glycoprotein	glycoprotein			N-glycans	Fterm		glycoprotein			It involves the attachment of an immune potentiator to CMP-Neu5AcN3 by Cu(I)-catalyzed azide-alkyne 1,3-cycloaddition followed by sialyltransferase-mediated transfer to N-glycans of a viral glycoprotein.
36830744	6	17	gly	sialylated	1118:1127	arg1	sialylated N-glycan structures				sialylated N-glycan structures						The most notable differences were observed in women, where the presence of sialylated N-glycan structures was negatively associated with CAD.
36493594	3	0	gly	glycosylation	788:800	arg2	a single glycosylation site			a single glycosylation site						site	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	46	gly	glycosylation	738:750	arg2	three glycosylation sites			three glycosylation sites						sites	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	75	gly	glycoproteins	697:709	arg1	two model mammalian glycoproteins	two model mammalian glycoproteins				Fterm		glycoproteins			METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	75	gly	glycoproteins	697:709	arg1	bovine fetuin	bovine fetuin				Fterm		fetuin			METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	75	gly	glycoproteins	697:709	arg1	human IgG	human IgG				Cterm		IgG			METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36637420	7	10	gly	non-glycosylated	1335:1350	arg1	non-glycosylated HAI-2	non-glycosylated HAI-2				PUBTATOR		HAI-2	10653		Unexpectedly, the vast majority of non-glycosylated HAI-2 is synthesized into multiple disulfide-linked oligomers, which lack protease inhibitory function, likely due to distorted conformations caused by the disarrayed disulfide linkages.
36056620	2	56	gly	glycosylation	252:264	arg1	viral envelope proteins	viral envelope proteins				Fterm		proteins			N-linked glycosylation of viral envelope proteins is a key mechanism for such evasion.
36409896	3	1	gly	glycoproteins	550:562	arg1	off-target glycoproteins	off-target glycoproteins				Fterm		glycoproteins			Here, we describe a method to identify and characterize a class of nanobodies that can distinguish glycoforms without reactivity to off-target glycoproteins or glycans.
35234142	0	54	gly	transglycosylation	35:52	arg1	the GH5_5 endo-1,4-β-glucanase RBcel1	the GH5_5 endo-1,4-β-glucanase RBcel1				Cterm		RBcel1			Highlighting the factors governing transglycosylation in the GH5_5 endo-1,4-β-glucanase RBcel1.
35871894	0	49	gly	glycoprotein	59:70	arg1	SARS-CoV-2 spike glycoprotein case study	SARS-CoV-2 spike glycoprotein case study				PUBTATOR		spike glycoprotein	43740568		Glycoprotein molecular dynamics analysis: SARS-CoV-2 spike glycoprotein case study.
36892535	8	34	gly	linked	1258:1263	arg1	MUC1 AND glycans	MUC1			glycans	OGER		MUC1	P15941		These experiments revealed only minor differences in peptide structure, therefore clearly relating the adhesion behaviour to the type and number of glycans linked to MUC1.
34102146	1	9	gly	glycoproteins	193:205	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Here, we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of matrix-assisted laser desorption ionization (MALDI) N-glycan mass spectrometry imaging (MSI) and spatially resolved glycoproteomics.
37235553	7	15	gly	N-glycans	1219:1227	arg1	this database	database			N-glycans	Fterm		database			Many N-glycans in this database are not found in current N-glycan mass spectrum libraries.
34223877	1	56	gly	Peanut	146:151	arg1	a carbohydrate-binding protein	Peanut			a carbohydrate-binding protein	Cterm		Peanut			Peanut agglutinin (PNA) is a carbohydrate-binding protein in peanuts that accounts for ~0.15% peanut weight.
37189353	4	7	gly	N-glycosylation	587:601	arg1	IgG	IgG				Cterm		IgG			To assess the changes in the N-glycosylation of the plasma proteins and IgG in atrial fibrillation, we analyzed the N-glycosylation of 172 patients with atrial fibrillation, before and six months after a pulmonary vein isolation procedure, with 54 cardiovascularly healthy controls.
37189353	4	7	gly	N-glycosylation	587:601	arg1	the plasma proteins	the plasma proteins				Fterm		proteins			To assess the changes in the N-glycosylation of the plasma proteins and IgG in atrial fibrillation, we analyzed the N-glycosylation of 172 patients with atrial fibrillation, before and six months after a pulmonary vein isolation procedure, with 54 cardiovascularly healthy controls.
36007953	3	45	gly	N-glycosylation	545:559	arg2	N-glycosylation sites			N-glycosylation sites						sites	In contrast, N-glycosylation sites are relatively invariant, and some are essential for infection.
35943155	6	19	gly	fucosylation	1212:1223	arg1	N-glycans				N-glycans						In addition, Fuc1584 also hydrolyzed fucosyl-α-1,6-N-acetylglucosamine (6FN), a component of the core fucosylation of N-glycans.
36959283	5	26	gly	B	894:894	arg1	quantitative N-glycan profiling	bovine pancreas ribonuclease B			quantitative N-glycan profiling	Cterm		bovine pancreas ribonuclease B			Qualitative and quantitative N-glycan profiling of purified human serum IgG, bovine serum fetuin, bovine pancreas ribonuclease B, blood-derived extracellular vesicle isolates, and total plasma results in the detection of >250, >400, >150, >310, and >520 N-glycans, respectively, using injected amounts equivalent to <25 ng of model protein and nL-levels of plasma-derived samples.
36959283	5	60	gly	IgG	839:841	arg1	quantitative N-glycan profiling	IgG			quantitative N-glycan profiling	Cterm		IgG			Qualitative and quantitative N-glycan profiling of purified human serum IgG, bovine serum fetuin, bovine pancreas ribonuclease B, blood-derived extracellular vesicle isolates, and total plasma results in the detection of >250, >400, >150, >310, and >520 N-glycans, respectively, using injected amounts equivalent to <25 ng of model protein and nL-levels of plasma-derived samples.
36959283	5	73	gly	fetuin	857:862	arg1	quantitative N-glycan profiling	fetuin			quantitative N-glycan profiling	Fterm		fetuin			Qualitative and quantitative N-glycan profiling of purified human serum IgG, bovine serum fetuin, bovine pancreas ribonuclease B, blood-derived extracellular vesicle isolates, and total plasma results in the detection of >250, >400, >150, >310, and >520 N-glycans, respectively, using injected amounts equivalent to <25 ng of model protein and nL-levels of plasma-derived samples.
37186866	3	52	gly	glycosylates	851:862	arg1	IPT domains			IPT domains						domains	Here, we report that the TMEM260 gene encodes an ER-located protein O-mannosyltransferase that selectively glycosylates IPT domains.
36239409	5	72	gly	K84	1401:1403	arg1	capsular polysaccharides	K84			capsular polysaccharides	OGER		K84	Q9NSB2		The structure of the tetrasaccharide repeating unit of the O-antigen is given by: →2)-β-d-Manp-(1→3)-β-d-Manp2Ac6Ac-(1→4)-β-d-GlcpA-(1→3)-α-d-GlcpNAc-(1→, which should also be the biological repeating unit and it shares structural elements with capsular polysaccharides from E. coli K84 and K50.
34695439	4	6	gly	glycosylation	657:669	arg1	proteins	proteins				Fterm		proteins			We aimed to start understanding the role of B3GLCT in AMD by evaluating its effect on glycosylation and secretion of proteins from retinal pigment epithelium (RPE) cells.
34510715	5	44	gly	enhancer	846:853	arg1	O-GlcNAc	enhancer of zeste homolog 2			O-GlcNAc	OGER		enhancer of zeste homolog 2	Q15910		PUGNAc treatment and cycloheximide (CHX) assay were performed to evaluate O-GlcNAc and the stabilization of the enhancer of zeste homolog 2 (EZH2).
32839225	0	41	gly	Asparagine	15:24	arg1	Sialylation			Asparagine 612	Sialylation			Inhibits Aconitase	P28271	Asparagine 612	Sialylation of Asparagine 612 Inhibits Aconitase Activity during Mouse Sperm Capacitation; a Possible Mechanism for the Switch from Oxidative Phosphorylation to Glycolysis.
32839225	3	50	gly	glycoproteins	599:611	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Regardless of its importance, to date, no single study has ever identified nor quantified which glycoproteins bearing terminal sialic acid (Sia) are altered during capacitation.
36828088	5	52	gly	PSP	585:587	arg1	The monosaccharide composition	PSP			The monosaccharide composition	OGER		PSP	O00186		The monosaccharide composition of PSP contained D-xylose, d-glucose, D-galactose with ratio of 1.0: 8.3: 1.3.
32296440	5	37	gly	glycosylated	895:906	arg1	the three glycosylated asparagines			the three glycosylated asparagines						asparagines	The role of SR-F1 N-linked glycans was also tested by mutating each of the three glycosylated asparagines.
36136114	2	63	gly	glycoprotein	260:271	arg1	glycoprotein therapeutic pharmacokinetics	glycoprotein therapeutic pharmacokinetics				Fterm		glycoprotein			Importantly, glycosylation modulates glycoprotein therapeutic pharmacokinetics, pharmacodynamics, and effector functions.
36437732	9	63	gly	fucosylated	1371:1381	arg1	biosynthetically related biantennary core fucosylated N-glycans				biosynthetically related biantennary core fucosylated N-glycans						The three glycans were biosynthetically related biantennary core fucosylated N-glycans that only varied by the degree of galactosylation (G0F, G1F, and G2F; G: galactose, F: fucose).
36437732	9	63	gly	fucosylated	1371:1381	arg1	The three glycans				The three glycans						The three glycans were biosynthetically related biantennary core fucosylated N-glycans that only varied by the degree of galactosylation (G0F, G1F, and G2F; G: galactose, F: fucose).
37341462	1	62	gly	O-glycopeptide	290:303	arg2	O-glycopeptide spectra			O-glycopeptide spectra						O-glycopeptide	Different from N-linked glycosylation, the core structures of mucin type O-glycans are much more diverse, and the sensitive interpretation of O-glycopeptide spectra remains a challenge.
37146275	5	20	gly	glycopeptides	1156:1168	arg2	glycopeptides			glycopeptides						glycopeptides	This microscopically highly hydrophilic material shows excellent enrichment ability for glycopeptides, such as extremely low detection limits (0.01 fmol μL-1), remarkable selectivity (1:8000), and size exclusion effects (1:8000).
32356523	2	12	gly	glycans	588:594	arg1	cargo glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
32356523	2	29	gly	glycoproteins	605:617	arg1	cargo glycoproteins	cargo glycoproteins				Fterm		glycoproteins			It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
32356523	2	37	gly	glycoproteins	675:687	arg1	cargo glycoproteins	cargo glycoproteins				Fterm		glycoproteins			It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
32356523	2	39	gly	ERGIC-53	541:548	arg1	the carbohydrate-recognition domain	ERGIC-53			the carbohydrate-recognition domain	PUBTATOR		ERGIC-53	3998		It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
36478308	13	56	gly	glycopeptide	1931:1942	arg2	our glycopeptide libraries			our glycopeptide libraries						glycopeptide	The use of our glycopeptide libraries greatly expedited the generation of site-specific glycan occupancy data for a large glycoprotein.
36478308	13	77	gly	glycoprotein	2038:2049	arg1	a large glycoprotein	a large glycoprotein				Fterm		glycoprotein			The use of our glycopeptide libraries greatly expedited the generation of site-specific glycan occupancy data for a large glycoprotein.
33801653	2	7	gly	cycling	370:376	arg1	substrate proteins	proteins			cycling	Fterm		proteins			Two enzymes are responsible for O-GlcNAc cycling on substrate proteins: O-GlcNAc transferase (OGT) catalyzes the addition while O-GlcNAcase (OGA) helps the removal of GlcNAc.
33552045	4	23	gly	glycosylated	597:608	arg1	glycosylated epitopes			glycosylated epitopes						epitopes	Although there is some evidence for T cell responses to glycosylated epitopes (glyco-epitopes) during viral infections in the literature, this aspect has been largely neglected for HIV.
32935560	5	4	gly	presence	885:892	arg2	N332 AND an N-linked glycan motif			N332	an N-linked glycan motif					N332	By analyzing glycosylation patterns in 82 subtype C viruses, we found that the presence of an N-linked glycan motif at position N413 and its absence at N332 were the most important predictors of neutralization resistance.
32935560	5	64	gly	position	925:932	arg1	an N-linked glycan motif			position N413	an N-linked glycan motif					position N413	By analyzing glycosylation patterns in 82 subtype C viruses, we found that the presence of an N-linked glycan motif at position N413 and its absence at N332 were the most important predictors of neutralization resistance.
36493594	9	4	gly	glycoprotein	1780:1791	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Thus, PF-ProA or PA-ProA allows for more effective identification and quantification of N-glycans than PF-AB in glycoprotein, particularly bovine fetuin.
33657401	1	46	gly	attached	136:143	arg2	proteins AND O-linked β-N-acetyl glucosamine	proteins			O-linked β-N-acetyl glucosamine	Fterm		proteins			O-linked β-N-acetyl glucosamine (O-GlcNAc) is attached to proteins under glucose-replete conditions; this posttranslational modification results in molecular and physiological changes that affect cell fate.
33657401	1	46	gly	attached	136:143	arg2	proteins AND O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			O-linked β-N-acetyl glucosamine (O-GlcNAc) is attached to proteins under glucose-replete conditions; this posttranslational modification results in molecular and physiological changes that affect cell fate.
37202422	5	48	gly	afucosylated	695:706	arg1	afucosylated IgG1	afucosylated IgG1				OGER		IgG1	P01857		Here, we present the structure of one such nanobody, X0, in complex with the Fc fragment of afucosylated IgG1.
32494619	3	13	gly	O-GlcNAcylation	590:604	arg1	serine-430			serine-430	serine-430		SpecificSite	interferon regulatory factor-5	Q13568	serine-430	Upon investigating the mechanisms driving this event, we determined that IAV induced OGT to bind to interferon regulatory factor-5 (IRF5), leading to O-GlcNAcylation of IRF5 on serine-430.
32494619	3	49	gly	IRF5	609:612	arg1	O-GlcNAcylation	IRF5			O-GlcNAcylation	OGER		IRF5	Q13568		Upon investigating the mechanisms driving this event, we determined that IAV induced OGT to bind to interferon regulatory factor-5 (IRF5), leading to O-GlcNAcylation of IRF5 on serine-430.