doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_base_np	arg_protein	arg_domain	arg_site	arg_sugar	PSource	SiteSource	NProtein	NID	SiteName	sent_text
36482940	2	32	gly	N-glycopeptide	524:537	arg2	the site- and structure-specific intact N-glycopeptide search engine GPSeeker			the site- and structure-specific intact N-glycopeptide search engine GPSeeker						N-glycopeptide	Methods: C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets.
36482940	2	94	gly	N-glycopeptides	435:449	arg2	labeled intact N-glycopeptides			labeled intact N-glycopeptides						N-glycopeptides	Methods: C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets.
35887202	3	5	gly	O-glycan	495:502	arg1	a specific Thr			a specific Thr	a specific Thr		AminoAcid			Thr	MY.1E12 is a promising anti-MUC1 antibody with a distinct specificity toward MUC1 modified with an immature O-glycan (NeuAcα(2-3)Galβ(1-3)GalNAc) on a specific Thr.
33073321	1	21	gly	glycopeptide	338:349	arg2	selective glycopeptide capture			selective glycopeptide capture						glycopeptide	A hydrophilic carbohydrate functionalized magnetic metal organic framework (Mag Zr-MOF@G6P) was synthesized via a facile one-step modification strategy for selective glycopeptide capture in virtue of hydrophilic interaction chromatography technique.
35977913	4	19	gly	N-acetylglucosamine	537:555	arg1	proteins	proteins			N-acetylglucosamine	Fterm		proteins			Utilizing the endoglycosidase-catalyzed transglycosylation method, a single N-acetylglucosamine (N-GlcNAc, analogous to a tree stump) on proteins can be converted to various homogeneous N-glycosylated forms, thereby becoming the focus of research efforts.
32839225	6	10	part_of	protein	1084:1090	arg1	N-linked sialoglycopeptides	52, testis-expressed protein 101		N-linked sialoglycopeptides		PUBTATOR	Site	52, testis-expressed protein 101	56746	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	14	part_of	zonadhesin	1100:1109	arg1	N-linked sialoglycopeptides	zonadhesin		N-linked sialoglycopeptides		PUBTATOR	Site	zonadhesin	Q9Y493	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	22	part_of	lipase	1026:1031	arg1	N-linked sialoglycopeptides	endothelial lipase		N-linked sialoglycopeptides		PUBTATOR	Site	endothelial lipase	16891	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	38	part_of	protein	1005:1011	arg1	N-linked sialoglycopeptides	C4b-binding protein		N-linked sialoglycopeptides		PUBTATOR	Site	C4b-binding protein	12269	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
37398203	9	37	part_of	motif	1641:1645	arg1	the antibody sequence	motif		the antibody sequence						sequence	This novel N -glycosylation site (absent in the germline sequence) is a result of a single mutation giving rise to an NDT motif in the antibody sequence.
37289534	4	11	gly	head	845:848	arg1	four or two saccharide head groups				four or two saccharide head groups						The E. coli LPS is found to form wormlike micelles, whereas the synthetic analogues bearing six lipid chains and with four or two saccharide head groups (Kdo2-lipid A and monophosphoryl lipid A) self-assemble into nanosheets or vesicles, respectively.
35414481	12	78	gly	fucosylated	1732:1742	arg1	tri-antennary fucosylated glycans				tri-antennary fucosylated glycans						The largest change over time was observed for tri-antennary fucosylated glycans, which were able to differentiate cases from controls with a specificity of 92%, sensitivity of 49% and accuracy of 90%.
35941485	3	49	part_of	CS/DS	632:636	arg1	CS/DS motifs	CS		CS/DS motifs		PUBTATOR	Site	CS	1431	motifs	The elevated structural diversity of CS/DS motifs, in which sulfation may occur at GalNAc and/or IdoA/GlcA in various combinations, requires the development of specific high performance analytical methods for reliable elucidation.
33161144	2	23	gly	glycosylation	375:387	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			It has been reported by our group and by others that different carbon sources, such as glucose, mannose and galactose, can differently impact the glycosylation profile of glycoproteins in mammalian cell culture.
33161144	2	41	gly	glycoproteins	400:412	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			It has been reported by our group and by others that different carbon sources, such as glucose, mannose and galactose, can differently impact the glycosylation profile of glycoproteins in mammalian cell culture.
35293727	9	19	part_of	O-antigen	1689:1697	arg1	O-antigen ISD fragments	O-antigen ISD		O-antigen ISD fragments		Cterm	Site	O-antigen ISD		fragments	MS/MS analysis of O-antigen ISD fragments allowed for the detection of specific repeat unit signatures.
35293727	9	81	part_of	ISD	1699:1701	arg1	O-antigen ISD fragments	O-antigen ISD		O-antigen ISD fragments		Cterm	Site	O-antigen ISD		fragments	MS/MS analysis of O-antigen ISD fragments allowed for the detection of specific repeat unit signatures.
36210615	3	48	part_of	contain	619:625	arg1	these enzymes AND several glycosylation sites	these enzymes		several glycosylation sites		Fterm	Site	enzymes		sites	Considering that metalloproteases and serine proteases are abundant components of Bothrops venoms and essential in the envenomation process, and that these enzymes contain several glycosylation sites, the role of sialic acid in venom proteolytic activity was evaluated.
36422041	10	62	gly	wall	1803:1806	arg1	composition			composition						position,	In conclusion, S. schenckii ROT2 is required for proper N-linked glycosylation, cell wall organization and composition, and interaction with the host.
35393560	2	0	gly	glycoproteins	194:206	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			Frequently, therapeutic glycoproteins exhibit a heterogeneous array of glycans that are intended to mimic human glycopatterns.
36830744	0	34	gly	N-Glycosylation	4:18	arg1	Coronary Artery Disease	Coronary Artery Disease				Fterm		Disease			IgG N-Glycosylation Is Altered in Coronary Artery Disease.
35995381	14	78	gly	sites	2060:2064	arg1	the sites Asn110, Asn1869, and Asn2122			sites Asn110, Asn1869, and Asn2122						sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn1869			sites Asn110, Asn1869, and Asn2122						sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn2122			sites Asn110, Asn1869, and Asn2122						sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn1869			sites Asn110, Asn1869, and Asn2122						sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn2122			sites Asn110, Asn1869, and Asn2122						sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35995381	14	78	gly	sites	2060:2064	arg1	Asn2122			sites Asn110, Asn1869, and Asn2122						sites Asn110, Asn1869, and Asn2122	N-glycan patterns for the sites Asn110, Asn1869, and Asn2122 were described for the first time in this current work.
35405095	5	36	gly	carrying	920:927	arg1	adjacent residues AND truncated core 1 O-glycans			adjacent residues	truncated core 1 O-glycans					residues	Here, we focus on the mucin-selective metalloprotease, Amuc_0627 (AM0627), which is known to cleave between adjacent residues carrying truncated core 1 O-glycans.
35460266	5	19	gly	regions	793:799	arg1	neutral polysaccharides			regions	neutral polysaccharides					regions	In this study, neutral polysaccharides of PR from four different regions of China (Chun'an (Zhejiang), Xixia (Henan), Danfeng (Shanxi), and Pan'an (Zhejiang)), named CAZJ, XXHN, DFSX, and PAZJ, respectively, were isolated by anion-exchange and gel-permeation chromatography.
35737823	1	15	gly	glycoprotein	192:203	arg1	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein				Fterm		glycoprotein			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein is the prime target for vaccines, diagnostics, and therapeutic antibodies against the virus.
36233110	0	86	gly	Protein	73:79	arg1	Site-Specific Glycan Microheterogeneity Evaluation	Protein			Site-Specific Glycan Microheterogeneity Evaluation	Fterm		Protein			Site-Specific Glycan Microheterogeneity Evaluation of Aflibercept Fusion Protein by Glycopeptide-Based LC-MSMS Mapping.
32417172	7	19	gly	glycosylated	1157:1168	arg1	the glycosylated sites			the glycosylated sites						sites	Using mass spectrometry analysis and site-directed mutagenesis, we identified the glycosylated sites and studied the functions of O-GalNAc glycosylation on p53.
37230634	7	9	part_of	positions	1023:1031	arg1	ME-2	ME-2		positions		OGER	Site	ME-2	P23368	positions	Secondly, the complex substituted positions of O-acetyl groups in ME-2 were determined to be at C-2, C-4, C-6 and C-4,6 in the backbone and at C-2 and C-2,3 in some side chains.
33904933	10	50	part_of	epitope	1831:1837	arg1	annexin A2	annexin A2		epitope		PUBTATOR	Site	annexin A2	302	epitope	• 12G5A antibody recognized the N-linked glycosylation epitope on annexin A2.
36812846	1	12	gly	glycoproteins	134:146	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Oligomannose-type glycans on glycoproteins are important signaling molecules in the glycoprotein quality control system in the endoplasmic reticulum.
36812846	1	55	gly	glycoprotein	189:200	arg1	the glycoprotein quality control system	the glycoprotein quality control system				Fterm		glycoprotein			Oligomannose-type glycans on glycoproteins are important signaling molecules in the glycoprotein quality control system in the endoplasmic reticulum.
36812846	1	63	gly	glycans	123:129	arg1	glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			Oligomannose-type glycans on glycoproteins are important signaling molecules in the glycoprotein quality control system in the endoplasmic reticulum.
33493676	10	5	gly	branching	1896:1904	arg1	AGP's second glycosylation site			AGP's second glycosylation site	AGP's second glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	5	gly	branching	1896:1904	arg1	AGP's third and AGP1's fourth glycosylation site			AGP's third and AGP1's fourth glycosylation site	AGP's third and AGP1's fourth glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	10	gly	glycosylation	1922:1934	arg2	AGP's second glycosylation site			AGP's second glycosylation site						site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	86	gly	glycosylation	2009:2021	arg2	AGP's third and AGP1's fourth glycosylation site			AGP's third and AGP1's fourth glycosylation site						site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's second glycosylation site			site	AGP's second glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site	AGP's second glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's second glycosylation site			site	AGP's third and AGP1's fourth glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site	AGP's third and AGP1's fourth glycosylation site		Site			site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's second glycosylation site			site	N-glycans					site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site	N-glycans					site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
33493676	10	95	gly	sialylation	1951:1961	arg1	AGP's third and AGP1's fourth glycosylation site			site						site	Individuals at higher risk of diabetes presented increased N-glycan branching on AGP's second glycosylation site and lower sialylation of N-glycans on AGP's third and AGP1's fourth glycosylation site.
34017182	5	65	gly	glycosylated	1162:1173	arg1	post-translational modified proteins	post-translational modified proteins				Fterm		proteins			Moreover, post-translational modified proteins (phosphorylated and N-linked glycosylated) were studied.
36409896	2	33	gly	glycoforms	219:228	arg1	complex protein glycoforms				complex protein glycoforms						However, interrogating complex protein glycoforms is challenging, as current lectin tools are limited by cross-reactivity while mass spectrometry typically requires biochemical purification and isolation of the target protein.
34029329	3	85	part_of	gp120	502:506	arg1	region-specific HIV-1 subtype C gp120 sequences	gp120		region-specific HIV-1 subtype C gp120 sequences		PUBTATOR	Site	gp120	3700	sequences	In the present study, we examined the diversity in genetic signatures and attributes that differentiate region-specific HIV-1 subtype C gp120 sequences associated with virus neutralization outcomes to key bnAbs having distinct epitope specificities.
36849074	2	7	gly	used	405:408	arg2	Composites			Composites						Composites	Composites within 0.08-0.25 wt% medium-viscosity alginate were used, at fixed PLA, 6.6 wt%, compared with a study using 0.17-0.48 wt% low-viscosity alginate (same PLA), starting from water-in-oil emulsions, before FS.
35945033	1	7	gly	glycoproteins	224:236	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The analysis of glycoproteins and the comparison of protein N-glycosylation from different eukaryotic origins require unbiased and robust analytical workflows.
32357974	2	100	part_of	HLA-bound	363:371	arg1	HLA-bound peptides	HLA		HLA-bound peptides		OGER	Site	HLA		peptides	Although immunopeptidomics studies routinely identify thousands of HLA-bound peptides from cell lines and tissue samples, in-depth analyses of the proportion and nature of peptides bearing one or more PTMs remains challenging.
32149134	10	70	part_of	CD147	1279:1283	arg1	CD147 peptide	CD147		CD147 peptide		PUBTATOR	Site	CD147	682	peptide	Furthermore, overexpression of GSDMD markedly overcame the inhibitory effect of CD147 peptide on tumor proliferation.
32993960	1	24	part_of	multi-membrane-spanning	163:185	arg1	multi-membrane-spanning regions	multi		multi-membrane-spanning regions		OGER	Site	multi		regions	Hyaluronan synthase 2 (HAS2) is an integral membrane protein composed of multi-membrane-spanning regions and a large intracellular loop (HAS2-loop).
31945375	7	55	gly	d-mannopyranose	1282:1296	arg1	the -1 subsite			the -1 subsite	the -1 subsite		Site			subsite	In addition, using two differential derivatization methods, we have shown that there is an absolute requirement for undecorated d-mannopyranose in the -1 subsite.
34957216	10	54	gly	glycosylation	1494:1506	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	We further predicted that many mutations on N-linked glycosylation sites would increase the stability of the S glycoprotein.
34102146	5	28	gly	glycopeptides	1015:1027	arg2	S- glycopeptides	proteins		glycopeptides		Fterm		proteins		glycopeptides	In total, our spatially resolved glycoproteomics technique identified over 400 N-, O-, and S- glycopeptides from over 30 proteins, demonstrating the diverse array of glycosylation present on the tissue slices and the sensitivity of our technique.
34778211	2	83	gly	presence	293:300	arg2	antibody Fc region AND core fucose			antibody Fc region	core fucose					region	The presence of core fucose on antibody Fc region can inhibit antibody-dependent cellular cytotoxicity (ADCC) and reduce antibody therapeutic efficiency in vivo.
36797772	3	8	part_of	peptide	751:757	arg1	the FAS1 domains	peptide		the FAS1 domains						domains	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	38	part_of	FAS1	720:723	arg1	the FAS1 domains	FAS1		the FAS1 domains		OGER	Site	FAS1	P25445	domains	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	80	part_of	FAS1	514:517	arg1	globular fasciclin-like (FAS1) domains	FAS1		globular fasciclin-like (FAS1) domains		OGER	Site	FAS1	P25445	domains	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	80	part_of	FAS1	514:517	arg1	a glycosylphosphatidylinositol (GPI)-anchor signal sequence	FAS1		a glycosylphosphatidylinositol (GPI)-anchor signal sequence		OGER	Site	FAS1	P25445	sequence	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	80	part_of	FAS1	514:517	arg1	a cleaved signal peptide	FAS1		a cleaved signal peptide		OGER	Site	FAS1	P25445	peptide	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	83	part_of	sequence	839:846	arg1	the FAS1 domains	sequence		the FAS1 domains						domains	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	75	part_of	containing	550:559	arg1	disordered sequences AND glycomotifs			glycomotifs						glycomotifs	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	75	part_of	containing	550:559	arg1	a glycosylphosphatidylinositol (GPI)-anchor signal sequence AND glycomotifs			glycomotifs						glycomotifs	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36797772	3	75	part_of	containing	550:559	arg1	a glycosylphosphatidylinositol (GPI)-anchor signal sequence AND glycomotifs			glycomotifs						glycomotifs	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
36824920	1	36	gly	glycoproteins	119:131	arg1	Coronavirus spike glycoproteins	Coronavirus spike glycoproteins				Fterm		glycoproteins			Coronavirus spike glycoproteins presented on the virion surface mediate receptor binding, and membrane fusion during virus entry and constitute the primary target for vaccine and drug development.
34399822	10	92	gly	enzymes	1419:1425	arg1	O-GlcNAcylation	enzymes			O-GlcNAcylation	Fterm		enzymes			Finally, the key enzymes in O-GlcNAcylation: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were examined and molecular docking analysis was used to determine effective natural compounds in the regulation on OGT and OGA activities.
35309180	14	47	gly	glycoproteins	1459:1471	arg1	The N-linked glycans	glycoproteins			The N-linked glycans	Fterm		glycoproteins			The N-linked glycans of glycoproteins were assessed to reveal chemotaxonomic patterns.
35662639	3	66	gly	glycoproteins	569:581	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We have shown that Bothrops venoms are markedly defined by their content of glycoproteins, and that most N-glycan structures of eight Bothrops venoms contain sialic acid, while bisected N-acetylglucosamine was identified in Bothrops cotiara venom.
36478308	10	14	gly	sites	1431:1435	arg1	N234			sites N61, N234						sites N61, N234	The majority of the glycans present were of the truncated class, although at sites N61, N234, and N717/714 high mannose structures were dominant and at N1173/1170 aglycosylation was dominant for both variant proteins.
36478308	10	14	gly	sites	1431:1435	arg1	N61			sites N61, N234						sites N61, N234	The majority of the glycans present were of the truncated class, although at sites N61, N234, and N717/714 high mannose structures were dominant and at N1173/1170 aglycosylation was dominant for both variant proteins.
36478308	10	14	gly	sites	1431:1435	arg1	N61			sites N61, N234						sites N61, N234	The majority of the glycans present were of the truncated class, although at sites N61, N234, and N717/714 high mannose structures were dominant and at N1173/1170 aglycosylation was dominant for both variant proteins.
33085102	2	21	gly	glycoprotein	342:353	arg1	different glycoprotein profiles	different glycoprotein profiles				Fterm		glycoprotein			This study reveals different glycoprotein profiles obtained from human, bovine, and caprine milk and their potential roles in supporting infant growth.
36095241	3	6	gly	glycopeptides	564:576	arg2	homogeneous O-linked glycopeptides			homogeneous O-linked glycopeptides						glycopeptides	Herein, an intrinsic O-glycosylated peptide P320-334 derived from RBD was screened and homogeneous O-linked glycopeptides containing Tn (GalNAcα1-O-Ser/Thr), T (Galβ1-3GalNAcα1-O-Ser/Thr), sialyl-Tn (sTn, Siaα2-6GalNAcα1-O-Ser/Thr), and sialyl-T (sT, Siaα2-3Galβ1-3GalNAcα1-O-Ser/Thr) structures were first synthesized via chemoenzymatic strategies.
36095241	3	39	gly	O-glycosylated	477:490	arg1	an intrinsic O-glycosylated peptide P320-334			an intrinsic O-glycosylated peptide P320-334						peptide	Herein, an intrinsic O-glycosylated peptide P320-334 derived from RBD was screened and homogeneous O-linked glycopeptides containing Tn (GalNAcα1-O-Ser/Thr), T (Galβ1-3GalNAcα1-O-Ser/Thr), sialyl-Tn (sTn, Siaα2-6GalNAcα1-O-Ser/Thr), and sialyl-T (sT, Siaα2-3Galβ1-3GalNAcα1-O-Ser/Thr) structures were first synthesized via chemoenzymatic strategies.
32545589	4	19	part_of	GnRH-R	830:835	arg1	Mizuhopecten yessoensis GnRH-R II sequences	GnRH-R II		Mizuhopecten yessoensis GnRH-R II sequences		OGER	Site	GnRH-R II	P30968	sequences	It shared 63%, 52%, and 30% sequence identities with Octopus vulgaris, Limulus polyphemus, and Mizuhopecten yessoensis GnRH-R II sequences, respectively.
32545589	4	70	part_of	II	837:838	arg1	Mizuhopecten yessoensis GnRH-R II sequences	GnRH-R II		Mizuhopecten yessoensis GnRH-R II sequences		OGER	Site	GnRH-R II	P30968	sequences	It shared 63%, 52%, and 30% sequence identities with Octopus vulgaris, Limulus polyphemus, and Mizuhopecten yessoensis GnRH-R II sequences, respectively.
34985300	4	14	part_of	sites	717:721	arg1	145 glycoproteins	glycoproteins		sites		Fterm	Site	glycoproteins		sites	We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
34985300	12	93	part_of	sites	2188:2192	arg1	145 proteins	proteins		sites		Fterm	Site	proteins		sites	This study has for the first time demonstrated the presence of O-linked glycosylation in this organism by determining the basic structure of the O-glycans and identifying 257 glycosylation sites in 145 proteins.
35481895	1	43	gly	glycosylation	136:148	arg1	structural proteins	structural proteins				Fterm		proteins			The glycosylation of structural proteins is a widespread posttranslational modification in Archaea.
37093892	6	76	part_of	carries	781:787	arg1	SU AND a variable region	SU		a variable region		Cterm	Site	SU		region	Within the viral envelope (Env), the surface protein (SU) carries a variable region that defines two genotypes, overlaps with the receptor binding domain (RBD), and is the exclusive target of nAbs.
37093892	6	76	part_of	carries	781:787	arg1	the surface protein AND a variable region	the surface protein		a variable region		Fterm	Site	protein		region	Within the viral envelope (Env), the surface protein (SU) carries a variable region that defines two genotypes, overlaps with the receptor binding domain (RBD), and is the exclusive target of nAbs.
32273875	5	88	gly	glycosylation	1014:1026	arg2	the N-linked glycosylation site			the N-linked glycosylation site						site	Production of the corresponding recombinant FVIII mutants or light chains indicated that removal of the N-linked glycosylation site at N2118 is sufficient to abrogate in vitro the activation of FVIII-specific CD4+ T cells by human monocyte-derived dendritic cells.
36824920	6	52	gly	glycosylation	1174:1186	arg2	N1242			N1242						N1242	We show that glycosylation at N1242 at the upper portion of the stalk is responsible for the extensive orientational freedom of the spike crown.
36385894	6	57	gly	sialylated	1097:1106	arg1	The N-glycans				The N-glycans						The N-glycans were mostly sialylated and sialofucosylated branched structures.
36329887	7	100	gly	derived	1365:1371	arg1	each FSH subunit AND Highly purified hFSH oligosaccharides	each FSH subunit			Highly purified hFSH oligosaccharides	Fterm		subunit			Highly purified hFSH oligosaccharides derived from each FSH subunit, were characterized by electrospray ionization-ion mobility-collision-induced dissociation (ESI-IM-CID) mass spectrometry.
34452239	3	31	gly	glycopeptide	715:726	arg2	The related O-linked glycopeptide			The related O-linked glycopeptide						glycopeptide	The related O-linked glycopeptide, Asp-Arg-Val-Tyr-Ile-His-Ser-(O-β-D-Glc)-amide (PNA5), is a biousian revision of the native peptide hormone Ang (1-7) and shows enhanced stability in vivo and greater levels of brain penetration.
34274643	7	60	gly	fucosylation	1180:1191	arg1	the N-linked glycans				the N-linked glycans						Results from these separation methods indicated that fucosylation of the N-linked glycans was more abundant when a high degree of branching was present in AGP.
33583770	7	31	gly	glycopeptides	1514:1526	arg2	enriched glycopeptides			enriched glycopeptides						glycopeptides	Furthermore, dynamic quantitative glycoproteomic analysis with multiplexed stable isotope labeling and analysis of enriched glycopeptides with multiple fragmentation approaches identified glycoproteins modified by these regulated glycans including several integrins and growth factor receptors.
33583770	7	83	gly	glycoproteins	1578:1590	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins	16852		Furthermore, dynamic quantitative glycoproteomic analysis with multiplexed stable isotope labeling and analysis of enriched glycopeptides with multiple fragmentation approaches identified glycoproteins modified by these regulated glycans including several integrins and growth factor receptors.
34878920	2	98	part_of	sites	327:331	arg1	gp85	gp85		sites		Cterm	Site	gp85		sites	In this study, we found that, among the 13 predicted N-linked glycosylation sites (NGSs) in gp85 of Tibetan chicken strain TBC-J6, N17, and N193/N191 are pivotal for virus replication.
35370997	3	1	part_of	Fc	646:647	arg1	the Fc fragment	Fc		the Fc fragment		Cterm	Site	Fc		fragment	The IgG structure and its effector functions are strongly dependent on the composition of N-glycans attached to the Fc fragment, and the binding of antigens is regulated by Fab sugar moieties.
36781790	4	46	gly	N-glycopeptides	986:1000	arg2	intact N-glycopeptides			intact N-glycopeptides						N-glycopeptides	The N-glycome information is then used to augment and guide the challenging reversed-phase LC-MS/MS-based profiling of intact N-glycopeptides from the same protein samples.
31644407	1	8	gly	glycosylation	203:215	arg1	plasma proteins	plasma proteins				Fterm		proteins			BACKGROUND A number of human inflammatory diseases and tumors have been shown to cause alterations in the glycosylation pattern of plasma proteins in a specific manner.
35278736	4	10	part_of	-α-l-Araf	1125:1133	arg1	→4)-α-l-GalpA (1→, →5)-α-l-Araf (1→ residues	Araf (1→		→4)-α-l-GalpA (1→, →5)-α-l-Araf (1→ residues		OGER	Site	Araf (1→	P10398	residues	As the most abundant and potent antioxidant component, the backbone of BJP-3 was mainly composed of →4)-α-l-GalpA (1→, →5)-α-l-Araf (1→ residues with two terminals of T-α-l-Araf (1→ and T-β-d-Galp (1→.
36136114	9	20	gly	glycopeptides	1463:1475	arg2	ulinastatin glycopeptides			ulinastatin glycopeptides						glycopeptides	By this methodology, we identified and characterized ulinastatin glycopeptides at the Fc domain and linker peptide.
32545589	3	46	gly	glycosylation	629:641	arg2	putative N-linked glycosylation motifs			putative N-linked glycosylation motifs						motifs	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
32545589	3	102	gly	serine	680:685	arg1	residues			residues						serine and threonine residues	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
35995381	3	102	part_of	Tg	461:462	arg1	human Tg N-glycosylation sites	Tg		human Tg N-glycosylation sites		PUBTATOR	Site	Tg	7038	sites	This study aimed to profile human Tg N-glycosylation sites and their glycan contents by using high-throughput glyco-analytical strategies, including glycopeptide and glycan levels.
35193013	9	15	gly	glycoproteins	1543:1555	arg1	BALB/c mouse intestinal lavages glycoproteins	BALB/c mouse intestinal lavages glycoproteins				Fterm		glycoproteins			BALB/c mouse intestinal lavages glycoproteins contained asialo N-glycans.
35193013	9	25	gly	contained	1557:1565	arg1	BALB/c mouse intestinal lavages glycoproteins AND asialo N-glycans	BALB/c mouse intestinal lavages glycoproteins			asialo N-glycans	Fterm		glycoproteins			BALB/c mouse intestinal lavages glycoproteins contained asialo N-glycans.
35943155	3	5	part_of	sequence	540:547	arg1	deduced amino acid sequences	sequence		deduced amino acid sequences						sequences	Twenty-two of those ORFs showed a complete coding sequence with deduced amino acid sequences displaying the highest degree of identity with α-l-fucosidases from Bacteroides thetaiotaomicron, Bacteroides caccae, Phocaeicola vulgatus, Phocaeicola dorei, Ruminococcus gnavus, and Streptococcus parasanguinis.
36182101	6	6	gly	carries	942:948	arg1	H. pylori strain J99 AND the sialic acid-binding adhesin	H. pylori strain J99			the sialic acid-binding adhesin	Fterm		strain			H. pylori strain J99, which carries the blood group antigen-binding adhesin (BabA), the sialic acid-binding adhesin (SabA), and the LacdiNAc-binding adhesin, bound both to Lewis b (Leb)-positive and Leb-negative mucins.
36182101	6	6	gly	carries	942:948	arg1	H. pylori strain J99 AND the LacdiNAc-binding adhesin	H. pylori strain J99			the LacdiNAc-binding adhesin	Fterm		strain			H. pylori strain J99, which carries the blood group antigen-binding adhesin (BabA), the sialic acid-binding adhesin (SabA), and the LacdiNAc-binding adhesin, bound both to Lewis b (Leb)-positive and Leb-negative mucins.
35848837	4	13	gly	glycoproteins	653:665	arg1	bacterial glycoproteins	bacterial glycoproteins				Fterm		glycoproteins			However, the glycan-protein linkages in bacteria are different and there is no enzyme available to release glycans from bacterial glycoproteins.
32760395	8	87	gly	glycosylation	1789:1801	arg1	the implantation site			the implantation site						site	In both groups, this spatiotemporal variation in the glycosylation pattern of the implantation site was accompanied by corresponding changes in galectin-1 expression.
36542493	4	50	gly	glycopeptides	495:507	arg2	glycopeptides			glycopeptides						glycopeptides	Herein, we chemically synthesized glycopeptides containing this atypical glycan structure and an absolute C6 configuration through the assembly of Ser O-Hep building blocks.
36542493	4	38	gly	containing	509:518	arg1	glycopeptides AND this atypical glycan structure			glycopeptides	this atypical glycan structure					glycopeptides	Herein, we chemically synthesized glycopeptides containing this atypical glycan structure and an absolute C6 configuration through the assembly of Ser O-Hep building blocks.
32790871	3	7	part_of	PfCSP	529:533	arg1	the N-terminal domain	CSP		the N-terminal domain		OGER	Site	CSP		domain	In contrast to the well-studied responses to the repeat region and the C-terminus, the antibody response against the N-terminal domain of PfCSP (N-CSP) remains obscure.
35773089	9	162	part_of	IgG	1884:1886	arg1	IgG glycome composition	IgG		IgG glycome composition		PUBTATOR	Site	IgG	668542	position	On the contrary, no statistically significant changes in IgG glycome composition were observed in patients with mild COVID-19.
36935145	1	56	gly	features	487:494	arg1	sequence			sequence						sequence	N-linked glycosylation (N-glycosylation) is a common protein post-translational modification, occurring on more than half of mammalian proteins; in striking contract with small molecule modifications (such as methylation, phosphorylation) with only single structures, N-glycosylation has multiple dimensional structural features (monosaccharide composition, sequence, linkage, anomer), which generates enormous N-glycan structures; and these structures widely regulate protein structure and functions.
35304921	4	16	gly	N-glycosylation	596:610	arg2	most potential N-glycosylation sites			most potential N-glycosylation sites						sites	Complex bi, tri, or tetraantennary N-glycans were predominant at various proportions at most potential N-glycosylation sites.
35304921	4	86	gly	predominant	543:553	arg2	most potential N-glycosylation sites AND Complex bi, tri, or tetraantennary N-glycans			most potential N-glycosylation sites	Complex bi, tri, or tetraantennary N-glycans					sites	Complex bi, tri, or tetraantennary N-glycans were predominant at various proportions at most potential N-glycosylation sites.
36852982	1	114	gly	glycosylated	278:289	arg1	other extracytoplasmic proteins	other extracytoplasmic proteins				Fterm		proteins			Neisseria meningitidis exhibits a general O-linked protein glycosylation system in which pili and other extracytoplasmic proteins are glycosylated.
34972858	8	99	gly	glycoproteins	1467:1479	arg1	complex glycan profiles	glycoproteins			complex glycan profiles	Fterm		glycoproteins			Following preliminary hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection separation and analysis, glycoproteins with complex glycan profiles were subjected to further fractionation by weak anion exchange HILIC and exoglycosidase sequential digestion for cross-validation of the glycan assignment.
36482940	3	9	gly	3,733 N-glycosites	876:893	arg2	3,733 N-glycosites			3,733 N-glycosites						3,733 N-glycosites	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	11	gly	glycopeptide	729:740	arg2	13,822 glycopeptide spectral matches			13,822 glycopeptide spectral matches						glycopeptide	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	21	gly	N-glycosite	812:822	arg2	comprehensive N-glycosite			comprehensive N-glycosite						N-glycosite	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	64	gly	sequence	914:921	arg1	3,754 N-glycan sequence structures				3,754 N-glycan sequence structures						Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
36482940	3	86	gly	2,918 N-glycoproteins	771:791	arg1	N-glycan structure information	2,918 N-glycoproteins			N-glycan structure information	Fterm		2,918 N-glycoproteins			Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
37266972	11	38	gly	glycovariants	1357:1369	arg1	N-glycan composition	glycovariants			N-glycan composition	Fterm		glycovariants			N-glycan composition of gB glycovariants was assessed by in vitro enzymatic mobility shift assay and proven to be consistent with the expected glycoforms.
36709835	5	16	gly	afucosylated	872:883	arg1	afucosylated glycans				afucosylated glycans						From these studies, we observed that Remicade® had the highest percent of afucosylated glycans (15.5 ± 1.3%) and the largest number of unique glycans, 28.
36399934	9	68	gly	site	1518:1521	arg1	Arg451			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Gln360			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ser370			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Gln360			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ser370			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ser370			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Leu450			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
36399934	9	68	gly	site	1518:1521	arg1	Ala368			Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451						Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451	Moreover, the resistance to inhibition by glucose was correlated with the presence of specific gatekeeper residues in the active site (Met204, Gln360, Ala368, Ser369, Ser370, Leu450, and Arg451).
37040463	6	42	gly	glycoprotein	743:754	arg1	a cell-free glycoprotein synthesis platform	a cell-free glycoprotein synthesis platform				Fterm		glycoprotein			To address this limitation, we develop a cell-free glycoprotein synthesis platform for building noncanonical glycans and, specifically, clickable azido-sialoglycoproteins (called GlycoCAP).
37040463	6	57	gly	azido-sialoglycoproteins	838:861	arg1	clickable azido-sialoglycoproteins	clickable azido-sialoglycoproteins				Fterm		azido-sialoglycoproteins			To address this limitation, we develop a cell-free glycoprotein synthesis platform for building noncanonical glycans and, specifically, clickable azido-sialoglycoproteins (called GlycoCAP).
36049519	8	53	part_of	motif	1272:1276	arg1	an active site	motif		an active site						site	Structural, kinetic, and mutagenic analyses point to a metzincin metalloprotease catalytic motif but with an active site that specifically recognizes a GalNAc residue α-linked to serine or threonine (i.e., the Tn-antigen).
37240090	0	51	gly	Glycoproteins	47:59	arg1	the Glycan Composition	Glycoproteins			the Glycan Composition	Fterm		Glycoproteins			Alterations in the Glycan Composition of Serum Glycoproteins in Attention-Deficit Hyperactivity Disorder.
37186866	4	7	part_of	IPT	957:959	arg1	IPT domains	IPT		IPT domains		OGER	Site	IPT	Q9H3H1	domains	We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knockout in cells results in receptor maturation defects and abnormal growth of 3D cell models.
37289618	2	1	gly	sialylated	398:407	arg1	intact sialylated N-linked glycans				intact sialylated N-linked glycans						Previous work has shown that infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) is able to detect intact sialylated N-linked glycans without the use of chemical derivatization.
36493594	6	38	gly	N-glycans	1109:1117	arg1	fetuin	fetuin			N-glycans	Fterm		fetuin			The N-glycans in fetuin (8-13 N-glycans were previously reported) and in IgG (19 N-glycans were previously reported), which could not be identified by using the widely used PF-AB, were all identified by using PF-ProA or PA-ProA.
32791164	11	23	gly	sialylated	1865:1874	arg1	O-linked sialylated keratan sulfate chains				O-linked sialylated keratan sulfate chains						Immunoblotting, immunohistochemistry, and enzyme treatments confirmed that Siglec-8 ligand on the human airway mucus layer is an isoform of DMBT1 carrying O-linked sialylated keratan sulfate chains (DMBT1S8).
36585837	8	65	gly	glycoproteins	1096:1108	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			ANOVA and Welch's t-test analysis identified glycoproteins differentially abundant between colostrum, transitional and mature milk (FDR <0.05).
36181511	3	14	gly	glycopeptide	648:659	arg2	overall glycopeptide enrichment			overall glycopeptide enrichment						glycopeptide	The comparison of cotton HILIC with Venusil HILIC and mixed anion-exchange (MAX) approaches indicated that cotton HILIC was superior in overall glycopeptide enrichment, whereas Venusil HILIC preferred in complex glycan structures and MAX performed better with high mannose glycans.
35191914	2	21	part_of	AAPs	449:452	arg1	the chemical compositions	AAPs		the chemical compositions		OGER	Site	AAPs	P55786	positions	The influences of different drying procedures on the chemical compositions and antioxidant activity in vitro and in vivo of AAPs were investigated.
37186866	2	67	gly	glycosylation	708:720	arg1	these IPT domains			these IPT domains						domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
35421698	4	6	gly	3,140 N-glycoproteins	731:751	arg1	3,140 N-glycoproteins	3,140 N-glycoproteins				Fterm		3,140 N-glycoproteins			With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
35421698	4	20	gly	5,687 N-glycopeptides	682:702	arg1	3,713 N-glycosites	3,140 N-glycoproteins		3,713 N-glycosites		Fterm		3,140 N-glycoproteins		3,713 N-glycosites	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
35421698	4	20	gly	5,687 N-glycopeptides	682:702	arg2	5,687 N-glycopeptides	3,140 N-glycoproteins		5,687 N-glycopeptides		Fterm		3,140 N-glycoproteins		5,687 N-glycopeptides	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
35421698	4	28	gly	3,713 N-glycosites	709:726	arg2	3,713 N-glycosites	3,140 N-glycoproteins		3,713 N-glycosites		Fterm		3,140 N-glycoproteins		3,713 N-glycosites	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
31996426	14	105	gly	glycoprotein	2247:2258	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein M			In contrast to several respiratory viruses and bacteria, EHV1 resisted potent antimicrobial equine β-defensins (eBDs) eBD2 and eBD3 by the action of glycoprotein M. Instead, eBD2 and -3 facilitated EHV1 particle aggregation and infection of rabbit kidney (RK13) cells.
35887202	7	42	part_of	MUC1	961:964	arg1	MUC1 glycopeptide	MUC1		MUC1 glycopeptide		PUBTATOR	Site	MUC1	P15941	glycopeptide	MD simulations of MUC1 glycopeptide showed that the O-glycosylation significantly limits the flexibility of the peptide backbone and side chain of the O-glycosylated Thr.
36373229	5	13	gly	O-glycopeptides	1096:1110	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	Here, we demonstrate how O-Pair Search, a recently developed O-glycopeptide-centric identification platform that enables rapid searches and confident O-glycosite localization, can be used to determine substrate specificities of various O-glycoproteases de novo from LC-MS/MS data of O-glycopeptides.
36373229	5	53	gly	O-glycosite	963:973	arg2	O-glycosite			O-glycosite						O-glycosite	Here, we demonstrate how O-Pair Search, a recently developed O-glycopeptide-centric identification platform that enables rapid searches and confident O-glycosite localization, can be used to determine substrate specificities of various O-glycoproteases de novo from LC-MS/MS data of O-glycopeptides.
36385894	8	77	gly	glycopeptides	1632:1644	arg2	glycopeptides			glycopeptides						glycopeptides	Biomarker models were constructed using serum N-glycans [area under the curve (AUC) = 0.775; 95% CI: 0.617-0.931] and glycopeptides (AUC = 0.959; 95% CI: 0.85-1.0), with glycopeptides having higher accuracies than N-glycans.
36385894	8	98	gly	glycopeptides	1684:1696	arg2	glycopeptides			glycopeptides						glycopeptides	Biomarker models were constructed using serum N-glycans [area under the curve (AUC) = 0.775; 95% CI: 0.617-0.931] and glycopeptides (AUC = 0.959; 95% CI: 0.85-1.0), with glycopeptides having higher accuracies than N-glycans.
35091091	1	56	gly	glycopeptide	178:189	arg2	complex glycopeptide samples			complex glycopeptide samples						glycopeptide	Rapidly improving methods for glycoproteomics have enabled increasingly large-scale analyses of complex glycopeptide samples, but annotating the resulting mass spectrometry data with high confidence remains a major bottleneck.
35293727	3	57	part_of	have	464:467	arg1	carrier proteins AND multiple glycosylation sites	carrier proteins		multiple glycosylation sites		Fterm	Site	proteins		sites	O-Antigen polysaccharides are typically polydisperse, and carrier proteins can have multiple glycosylation sites.
36493594	2	2	gly	glycoproteins	584:596	arg1	mammalian glycoproteins	mammalian glycoproteins				Fterm		glycoproteins			OBJECTIVE To obtain consistent results for qualitative and quantitative analyses of N-glycans, N-glycans obtained by different preparation methods were compared for two types of mammalian glycoproteins.
36253324	3	0	gly	α1,3-fucosylation	451:467	arg1	complex glycans				complex glycans						Here, we report a facile and general redox-controlled substrate engineering strategy for the site-specific α1,3-fucosylation of complex glycans containing multiple LacNAc units.
36631484	4	71	gly	glycoprotein	646:657	arg1	glycoprotein production	glycoprotein production				Fterm		glycoprotein			Notably, many factors can affect the composition of the glycans during glycoprotein production, and variations in glycosylation are among the leading causes of pharmaceutical batch rejection.
31657490	9	28	gly	glycopeptides	1527:1539	arg2	glycopeptides			glycopeptides						glycopeptides	It has great potential in the enrichment of glycopeptides and provides new ideas for synthetic materials with better enrichment properties in the future.
36892535	5	11	part_of	MUC1	777:780	arg1	up to 6 differently glycosylated MUC1 peptides	MUC1		up to 6 differently glycosylated MUC1 peptides		OGER	Site	MUC1	P15941	peptides	Cell adhesion experiments with MCF-7 cancer cells on surfaces carrying up to 6 differently glycosylated MUC1 peptides demonstrated that different glycans significantly impact adhesion.
36870654	1	18	gly	glycosylation	97:109	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The role of glycosylation in the binding of glycoproteins to carbohydrate substrates has not been well understood.
36870654	1	60	gly	glycoproteins	129:141	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The role of glycosylation in the binding of glycoproteins to carbohydrate substrates has not been well understood.
35943155	10	101	gly	glycoprotein	1735:1746	arg1	the glycoprotein mucin	the glycoprotein mucin				Fterm		glycoprotein			This latest enzyme also displayed activity in glycoconjugates carrying lacto-N-fucopentaose II (Lea) and lacto-N-fucopentaose III (Lex) and in the glycoprotein mucin.
33184803	1	33	part_of	metalloproteinase	317:333	arg1	a thrombospondin type 1 motif	metalloproteinase		a thrombospondin type 1 motif		Fterm	Site	metalloproteinase		motif	BACKGROUND Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a potentially fatal blood disorder, resulting from autoantibodies against ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13).
33184803	1	49	part_of	type	357:360	arg1	a thrombospondin type 1 motif	thrombospondin type 1		a thrombospondin type 1 motif		OGER	Site	thrombospondin type 1	P07996	motif	BACKGROUND Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a potentially fatal blood disorder, resulting from autoantibodies against ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13).
33184803	1	52	part_of	disintegrin	301:311	arg1	a thrombospondin type 1 motif	disintegrin		a thrombospondin type 1 motif		Fterm	Site	disintegrin		motif	BACKGROUND Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is a potentially fatal blood disorder, resulting from autoantibodies against ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13).
35320529	8	52	gly	glycosylation	1404:1416	arg1	endogenous membrane receptor	receptor		sites		Fterm		receptor		sites	Thus, the protocol described in this section can be utilized to identify galectin ligands at specific glycosylation sites of endogenous membrane receptor from circulating primary human lymphocytes.
35104505	0	22	gly	glycoprotein	106:117	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			N-acetylglucosaminyltransferase-V requires a specific noncatalytic luminal domain for its activity toward glycoprotein substrates.
36478308	2	28	gly	glycosylation	407:419	arg2	single, isolated glycosylation sites			single, isolated glycosylation sites						sites	The method has been optimised to produce peptides with single, isolated glycosylation sites using multiple protease digests.
36056620	8	100	gly	glycosylation	1365:1377	arg2	N-linked glycosylation motifs			N-linked glycosylation motifs						motifs	CONCLUSIONS We show how N-linked glycosylation motifs can trigger dramatic changes in HCV sensitivity to nAb, independent of glycan occupancy.
33085102	7	24	gly	glycoproteins	1010:1022	arg1	17 glycoproteins	17 glycoproteins				Fterm		glycoproteins			Among those identified, human milk has 17 glycoproteins, which are both O- and N-glycosylated, whereas caprine and bovine have 10 and 1, respectively.
35504880	1	16	gly	glycoproteins	234:246	arg1	many glycoproteins	many glycoproteins				Fterm		glycoproteins	34215		C1GalT1 is an essential inverting glycosyltransferase responsible for synthesizing the core 1 structure, a common precursor for mucin-type O-glycans found in many glycoproteins.
35504880	1	26	gly	found	220:224	arg1	many glycoproteins AND mucin-type O-glycans	many glycoproteins			mucin-type O-glycans	Fterm		glycoproteins	34215		C1GalT1 is an essential inverting glycosyltransferase responsible for synthesizing the core 1 structure, a common precursor for mucin-type O-glycans found in many glycoproteins.
31657490	4	18	gly	glycopeptide	708:719	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	The obtained material was used for glycopeptide enrichment.
31657490	1	7	gly	Glycosylation	131:143	arg1	proteins	proteins				Fterm		proteins			RATIONALE Glycosylation of proteins plays an important role in life activities, but the concentration of naturally occurring glycopeptides is usually relatively low, and glycosylation has microfacies heterogeneity, so direct mass spectrometry is not feasible.
31657490	1	24	gly	glycopeptides	246:258	arg2	naturally occurring glycopeptides			naturally occurring glycopeptides						glycopeptides	RATIONALE Glycosylation of proteins plays an important role in life activities, but the concentration of naturally occurring glycopeptides is usually relatively low, and glycosylation has microfacies heterogeneity, so direct mass spectrometry is not feasible.
35091091	2	78	gly	glycopeptides	421:433	arg2	glycopeptides			glycopeptides						glycopeptides	We recently introduced a fast and sensitive glycoproteomics search method in our MSFragger search engine, which reports glycopeptides as a combination of a peptide sequence and the mass of the attached glycan.
36124803	4	22	gly	glycoprotein	687:698	arg1	our glycoprotein database	our glycoprotein database				Fterm		glycoprotein			We show how to enhance site annotation with federated queries involving UniProt and GlyConnect, our glycoprotein database.
35766466	9	14	gly	nonsialylated	1630:1642	arg1	nonsialylated N-glycans				nonsialylated N-glycans						Sialidase utilization in MALDI-IMS dramatically increases sensitivity and increases on-tissue endoglycosidase efficiency, making it a very useful companion technique to specifically detect nonsialylated N-glycans.
36192063	8	75	gly	sialylated	1535:1544	arg1	307 sialylated intact N-glycopeptides			307 sialylated intact N-glycopeptides						N-glycopeptides	In total, 307 sialylated intact N-glycopeptides with linkage-specific sialic acid residues were identified together with 29 N-glycans with α2,6-linked sialic acids and 55 N-glycans with α2,3-linked sialic acids.
36192063	8	78	gly	N-glycopeptides	1553:1567	arg2	307 sialylated intact N-glycopeptides			N-glycopeptides	linkage-specific sialic acid residues					N-glycopeptides	In total, 307 sialylated intact N-glycopeptides with linkage-specific sialic acid residues were identified together with 29 N-glycans with α2,6-linked sialic acids and 55 N-glycans with α2,3-linked sialic acids.
37316505	6	0	part_of	sites	957:961	arg1	the PD-1 protein	PD-1 protein		sites		OGER	Site	PD-1 protein	Q15116	sites	This study provides both information on potential novel modification sites on the PD-1 protein and an attractive method for identifying O-linked glycosylation using a specific enzyme and intact mass analysis.
36399685	4	5	gly	N-glycoprotein	742:755	arg1	cell-free N-glycoprotein synthesis	cell-free N-glycoprotein synthesis				Fterm		N-glycoprotein			The ability to generate glycosylated ribosome-nascent chain (glycoRNC) complexes was enabled by integrating SecM-mediated translation arrest with methods for cell-free N-glycoprotein synthesis.
35696078	1	23	part_of	protein	185:191	arg1	an amino acid residue	protein		an amino acid residue		Fterm	Site	protein		residue	Glycosylation involves the attachment of carbohydrate sugar chains, or glycans, onto an amino acid residue of a protein.
35370997	1	28	gly	glycoprotein	253:264	arg1	the most abundant glycoprotein	the most abundant glycoprotein				Fterm		glycoprotein			The N-glycome of immunoglobulin G (IgG), the most abundant glycoprotein in human blood serum, reflects pathological conditions of autoimmunity and is sensitive to medicines applied in disease therapy.
32796070	10	26	gly	glycoprotein	1694:1705	arg1	the HIV-1 glycoprotein conformation	the HIV-1 glycoprotein conformation				Fterm		glycoprotein			Defining the specific effects of SERINC5 on the HIV-1 glycoprotein conformation may be useful for designing new antiviral strategies targeting Env.
36055406	0	41	part_of	GPI-attachment	120:133	arg1	C-terminal GPI-attachment sequence	GPI		C-terminal GPI-attachment sequence		OGER	Site	GPI	P06744	sequence	ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
35717753	4	4	gly	L-His	563:567	arg1	(Cu2+)(A)(L-His)-H]+ and the mononuclear copper-bound quaternary ions [(Cu2+)(A)(L-Ser)3-H]+ (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine			His	(Cu2+)(A)(L-His)-H]+ and the mononuclear copper-bound quaternary ions [(Cu2+)(A)(L-Ser)3-H]+ (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine					His	In this study, two novel complex ions, the mononuclear copper-bound dimeric ions [(Cu2+)(A)(L-His)-H]+ and the mononuclear copper-bound quaternary ions [(Cu2+)(A)(L-Ser)3-H]+ (where A denotes a disaccharide, and L-Ser/His denotes l-serine/histidine), were designed for the collision-induced dissociation-based identification and relative quantification of 14 disaccharide isomers.
36825496	2	2	gly	fucosylated	181:191	arg1	fucosylated glycans				fucosylated glycans						A main obstacle to the sequencing of fucosylated glycans is the migration of fucose, which leads to misinterpretations in mass spectrometry analysis.
36813234	0	11	gly	P-glycoprotein	74:87	arg1	P-glycoprotein expression	P-glycoprotein expression				Fterm		P-glycoprotein			Expression of GnT-III decreases chemoresistance via negatively regulating P-glycoprotein expression: Involvement of the TNFR2-NF-κB signaling pathway.
35945033	2	43	gly	asparagine-linked	609:625	arg1	N-glycans			asparagine	N-glycans					asparagine	The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35945033	2	43	gly	asparagine-linked	609:625	arg1	asparagine-linked oligosaccharides			asparagine	asparagine-linked oligosaccharides					asparagine	The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35945033	2	129	gly	glycoproteins	661:673	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35945033	2	129	gly	glycoproteins	661:673	arg1	asparagine-linked oligosaccharides	glycoproteins			asparagine-linked oligosaccharides	Fterm		glycoproteins			The structural and functional analysis of vertebrate protein N-glycosylation currently depends extensively on bacterial peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases), which are indispensable enzymatic tools in releasing asparagine-linked oligosaccharides (N-glycans) from glycoproteins.
35248671	1	34	gly	glycoproteins	137:149	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			BACKGROUND N-Glycan branching regulates various functions of glycoproteins.
32357974	5	70	gly	glycosylation	939:951	arg2	the N-linked glycosylation motif NX(S/T)			the N-linked glycosylation motif NX(S/T)						motif	Notably, we found that the N-linked glycosylation motif NX(S/T) was highly abundant across asparagine-deamidated HLA-bound peptides.
36478308	5	34	gly	glycosylation	823:835	arg2	isolated glycosylation sites			isolated glycosylation sites						sites	Three different protease digests were utilised to generate peptides with isolated glycosylation sites.
37266972	13	73	gly	deglycosylated	1655:1668	arg1	deglycosylated pharmaceutical proteins	deglycosylated pharmaceutical proteins				Fterm		proteins			Production of deglycosylated pharmaceutical proteins in BY-2 cells expands the set of glycoengineered BY-2 cell lines.
35343965	5	41	gly	glycoproteins	826:838	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			However, despite the advances made in the understanding of glycan composition, structure, and biosynthesis pathways, understanding of the role of glycoproteins in pathogenicity or interaction with the environment remains very limited.
36380917	0	13	gly	sialylation	62:72	arg1	glucagon-like peptide 1			glucagon-like peptide 1						peptide	Identification of the effect of N-glycan modification and its sialylation on proteolytic stability and glucose-stabilizing activity of glucagon-like peptide 1 by site-directed enzymatic glycosylation.
36380917	0	40	gly	peptide	149:155	arg1	glucose-stabilizing activity			peptide	glucose-stabilizing activity					peptide	Identification of the effect of N-glycan modification and its sialylation on proteolytic stability and glucose-stabilizing activity of glucagon-like peptide 1 by site-directed enzymatic glycosylation.
35943155	11	77	gly	glycoprotein	1843:1854	arg1	human α-1 acid glycoprotein	human α-1 acid glycoprotein				Fterm		glycoprotein			Fuc18, Fuc19A, and Fuc39 also removed l-fucose from neoglycoproteins and human α-1 acid glycoprotein.
35943155	11	130	gly	neoglycoproteins	1807:1822	arg1	neoglycoproteins	neoglycoproteins				Fterm		neoglycoproteins			Fuc18, Fuc19A, and Fuc39 also removed l-fucose from neoglycoproteins and human α-1 acid glycoprotein.
35104505	8	11	gly	glycoproteins	1511:1523	arg1	substrate glycoproteins	substrate glycoproteins				Fterm		glycoproteins			Finally, docking models of GnT-V with substrate glycoproteins suggested that the N domain could interact with the substrate polypeptide directly.
36933625	10	50	part_of	chitin	1475:1480	arg1	a chitin insertion domain	chitin		a chitin insertion domain		Fterm	Site	chitin		domain	CONCLUSIONS Physarum polycephalum sequences encompassing a chitinase like GH18 signature share a modular structure involving a structurally conserved catalytic TIM barrels decorated or not by a chitin insertion domain and optionally surrounded by additional sugar binding domains.
35670884	6	12	gly	P261	1084:1087	arg1	residues			residues						residues	Molecular dynamics analysis revealed that core fucosylation of SPARC-induced dynamic conformational changes in its N-glycan, allowing terminal galactose and N-acetylglucosamine to interact with K150, P261 and H264 residues, thereby promoting collagen binding.
36482940	3	86	part_of	2,918 N-glycoproteins	771:791	arg1	comprehensive N-glycosite	2,918 N-glycoproteins		comprehensive N-glycosite		Fterm	Site	2,918 N-glycoproteins		N-glycosite	Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively.
34778211	5	115	gly	glycoprotein	777:788	arg1	host cell glycoprotein impurities	host cell glycoprotein impurities				Fterm		glycoprotein			The characterization of monoclonal antibodies as well as host cell glycoprotein impurities are required for quality control purposes under regulation rules.
36754227	2	1	part_of	position	339:346	arg1	SV2A	SV2A		position		OGER	Site	SV2A	Q7L0J3	position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	2	part_of	position	252:259	arg1	SV2C	SV2C		position		OGER	Site	SV2C	Q496J9	position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	1	part_of	position	339:346	arg1	LD-SV2C	SV2C		position		OGER	Site	SV2C	Q496J9	position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	2	part_of	position	252:259	arg1	LD-SV2A	SV2A		position		OGER	Site	SV2A	Q7L0J3	position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	26	part_of	domain	289:294	arg1	position N573			position						position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	27	part_of	domain	202:207	arg1	position N573			position						position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	74	part_of	SV2A	212:215	arg1	the luminal domain	SV2A		domain		OGER	Site	SV2A	Q7L0J3	domain	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	76	part_of	SV2C	299:302	arg1	the luminal domain	SV2C		domain		OGER	Site	SV2C	Q496J9	domain	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36482940	6	47	gly	N-glycoprotein	1491:1504	arg1	putative N-glycoprotein biomarker candidates	putative N-glycoprotein biomarker candidates				Fterm		N-glycoprotein			This discovery study provides putative N-glycoprotein biomarker candidates for future validation study using multiple reaction monitoring and biochemical methods.
35696078	7	17	part_of	sites	973:977	arg1	proteins	proteins		sites		Fterm	Site	proteins		sites	With the development of glycan-related databases and a glycan repository, bioinformatics approaches have attempted to predict the glycosylation pathway and the glycosylation sites on proteins.
33515675	10	30	part_of	Notch	1473:1477	arg1	the Notch intracellular domain	Notch		the Notch intracellular domain		PUBTATOR	Site	Notch	4851	domain	We further clarified the molecular mechanisms by which EOGT and SHCBP1 enhance the O-GlcNAcylation of NOTCH1, Subsequently promoting the nuclear localization of the Notch intracellular domain (NICD) and inhibiting the transcription of E-cadherin and P21 in pancreatic cancer cells.
37211816	2	29	gly	N-glycosylation	473:487	arg2	an N-glycosylation N-X-T sequon			an N-glycosylation N-X-T sequon						sequon	To further increase the versatility of VHHs, we explored the VHH scaffold in a structure-guided approach to select regions where the introduction of an N-glycosylation N-X-T sequon and its associated glycan should not interfere with protein folding or epitope recognition.
37294165	6	12	part_of	SPINK13	1183:1189	arg1	the full-length polypeptide	SPINK13		the full-length polypeptide		OGER	Site	SPINK13	Q1W4C9	polypeptide	This insertion strategy successfully afforded the full-length polypeptide of SPINK13 within two steps from glycosylated asparagine thioacid.
36324280	1	10	part_of	protein	136:142	arg1	four N-glycosylation sites	protein		four N-glycosylation sites		Fterm	Site	protein		sites	Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans.
37162352	7	39	gly	glycosylated	966:977	arg1	The glycosylated sites			The glycosylated sites						sites	The glycosylated sites were predominantly located in the periplasm, where they are postulated to be beneficial for protein folding/stability.
33078708	3	8	gly	glycosylated	511:522	arg1	an O-linked glycosylated protein	an O-linked glycosylated protein				Fterm		protein			Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca2+ and phosphorylation cascades.
32357974	5	37	part_of	HLA-bound	1016:1024	arg1	asparagine-deamidated HLA-bound peptides	HLA		asparagine-deamidated HLA-bound peptides		OGER	Site	HLA		peptides	Notably, we found that the N-linked glycosylation motif NX(S/T) was highly abundant across asparagine-deamidated HLA-bound peptides.
35077806	3	12	gly	glycoproteins	547:559	arg1	their homing secreted glycoproteins	their homing secreted glycoproteins				Fterm		glycoproteins			Despite the numerous studies aiming at the biochemical characterization of anuran skin secretions, little is known about protein-linked oligosaccharides, their synthesis pathways, and their homing secreted glycoproteins.
36660462	6	20	gly	glycopeptides	1057:1069	arg2	123 differently expressed glycopeptides			123 differently expressed glycopeptides						glycopeptides	A total of 123 differently expressed glycopeptides, which were from 47 glycoproteins were identified among three groups.
36660462	6	23	gly	glycoproteins	1091:1103	arg1	47 glycoproteins	47 glycoproteins				Fterm		glycoproteins			A total of 123 differently expressed glycopeptides, which were from 47 glycoproteins were identified among three groups.
37148536	10	133	gly	EPO-Fc sialylation	1829:1846	arg1	tri-				tri-						Inhibitor supplementation also led to a decrease in bi-, tri-, and tetra-antennary structures and up to 50% lower EPO-Fc sialylation.
37148536	10	133	gly	EPO-Fc sialylation	1829:1846	arg1	bi-				bi-						Inhibitor supplementation also led to a decrease in bi-, tri-, and tetra-antennary structures and up to 50% lower EPO-Fc sialylation.
37148536	10	133	gly	EPO-Fc sialylation	1829:1846	arg1	tetra-antennary structures				tetra-antennary structures						Inhibitor supplementation also led to a decrease in bi-, tri-, and tetra-antennary structures and up to 50% lower EPO-Fc sialylation.
35775947	4	13	part_of	-β-Galp	952:958	arg1	the 1-S-α-Galp(1 → 3)-β-Galp motif	Galp		the 1-S-α-Galp(1 → 3)-β-Galp motif		OGER	Site	Galp	Q9UBC7	motif	With this purpose, we addressed the synthesis of a trisaccharide and two isomeric tetrasaccharides containing the 1-S-α-Galp(1 → 3)-β-Galp motif, the thio analog of the epitope recognized by lytic antibodies.
35775947	4	61	part_of	1-S-α-Galp	935:944	arg1	the 1-S-α-Galp(1 → 3)-β-Galp motif	Galp		the 1-S-α-Galp(1 → 3)-β-Galp motif		OGER	Site	Galp	Q9UBC7	motif	With this purpose, we addressed the synthesis of a trisaccharide and two isomeric tetrasaccharides containing the 1-S-α-Galp(1 → 3)-β-Galp motif, the thio analog of the epitope recognized by lytic antibodies.
32169063	4	7	gly	bunit	662:666	arg1	sequences			sequences						sequences	RESULTS Two novel long-acting recombinant human IFN-α2b (rhIFN-α2b) proteins were designed in which the carboxyl-terminal peptide (CTP) of the human chorionic gonadotropin β su bunit and N-linked glycosylation sequences were linked to rhIFN-α2b.
36935145	2	15	gly	N-glycosylation	696:710	arg2	the Asn residue			the Asn residue						residue	For the modification site, N-glycosylation occurs on the Asn residue among the consensus N-X-S/T/C (X≠P) motif; mutation-originated amino acid change may lead to loss of such an original motif and thus loss-of-glycosylation (LoG) or gain of such a new motif and thus gain-of-glycosylation (GoG).
37354227	2	51	gly	glycosylated	287:298	arg1	Viral proteins	Viral proteins				Fterm		proteins			Viral proteins used in cell entry are often extensively glycosylated to assist in protein folding, provide stability, and shield the virus from immune recognition by its host (described as a "glycan shield").
32357974	9	11	gly	deglycosylation	1819:1833	arg1	formerly glycosylated proteins	formerly glycosylated proteins				Fterm		proteins			This indicates that a large proportion of HLA-I bound asparagine deamidated peptides are generated from formerly glycosylated proteins that have undergone deglycosylation via the ER-associated protein degradation (ERAD) pathway.
32357974	9	60	gly	glycosylated	1777:1788	arg1	formerly glycosylated proteins	formerly glycosylated proteins				Fterm		proteins			This indicates that a large proportion of HLA-I bound asparagine deamidated peptides are generated from formerly glycosylated proteins that have undergone deglycosylation via the ER-associated protein degradation (ERAD) pathway.
36637420	5	11	gly	N-glycosylation	918:932	arg2	two putative N-glycosylation sites			sites, Asn-57 and Asn-94						sites, Asn-57 and Asn-94	HAI-2 contains two putative N-glycosylation sites, Asn-57 and Asn-94, point mutations of which were generated and characterized in this study.
36637420	5	11	gly	N-glycosylation	918:932	arg2	Asn-94			sites, Asn-57 and Asn-94						sites, Asn-57 and Asn-94	HAI-2 contains two putative N-glycosylation sites, Asn-57 and Asn-94, point mutations of which were generated and characterized in this study.
36637420	5	11	gly	N-glycosylation	918:932	arg2	Asn-94			sites, Asn-57 and Asn-94						sites, Asn-57 and Asn-94	HAI-2 contains two putative N-glycosylation sites, Asn-57 and Asn-94, point mutations of which were generated and characterized in this study.
36542493	5	3	gly	glycopeptides	641:653	arg2	glycopeptides			glycopeptides						glycopeptides	Using glycopeptides as haptens, we generated first-in-class poly- and monoclonal antibodies, termed Anti-SerHep1a and Anti-SerHep1b, that stereoselectively recognize Ser O-heptosylation (d/l-glycero) with high specificity in vitro and in vivo.
36329887	5	72	gly	attached	878:885	arg1	FSHα Asn<sup>52</sup> AND a single biantennary glycan			FSHα Asn<sup>52</sup>	a single biantennary glycan					Asn	It was noted that a single biantennary glycan attached to FSHα Asn<sup>52</sup> might preemptively fill the central pocket in this complex and prevent the other two FSH ligands from binding the remaining ligand-binding sites.
36388954	2	9	gly	N-glycosylation	296:310	arg2	five distinct N-glycosylation sites			five distinct N-glycosylation sites						sites	We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome.
36388954	2	45	gly	protein	269:275	arg1	intracellular, site-specific glycan processing	protein			intracellular, site-specific glycan processing	Fterm		protein			We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome.
36233110	6	33	part_of	IgG	1430:1432	arg1	the IgG Fc domain site	IgG		the IgG Fc domain site		Cterm	Site	IgG		site	The presence of N-glycolylneuraminic acid (NeuGc) glycans, which may mediate adverse immune reactions in antibody therapeutics, were also detected on the sites of VEGFR1 and VEGFR2 domains, but not on the IgG Fc domain site.
36233110	6	69	part_of	VEGFR2	1399:1404	arg1	VEGFR1 and VEGFR2 domains	VEGFR2		VEGFR1 and VEGFR2 domains		PUBTATOR	Site	VEGFR2	3791	domains	The presence of N-glycolylneuraminic acid (NeuGc) glycans, which may mediate adverse immune reactions in antibody therapeutics, were also detected on the sites of VEGFR1 and VEGFR2 domains, but not on the IgG Fc domain site.
36233110	6	70	part_of	VEGFR1	1388:1393	arg1	VEGFR1 and VEGFR2 domains	VEGFR1		VEGFR1 and VEGFR2 domains		PUBTATOR	Site	VEGFR1	2321	domains	The presence of N-glycolylneuraminic acid (NeuGc) glycans, which may mediate adverse immune reactions in antibody therapeutics, were also detected on the sites of VEGFR1 and VEGFR2 domains, but not on the IgG Fc domain site.
32790871	0	32	part_of	CSP	37:39	arg1	the CSP N-terminal domain	CSP		the CSP N-terminal domain		OGER	Site	CSP	Q9H3Z4	domain	A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity.
37272940	5	36	part_of	elastin-like	655:666	arg1	elastin-like polypeptides	elastin		elastin-like polypeptides		OGER	Site	elastin	P15502	polypeptides	SCE was composed of SpyCatcher (SC) and elastin-like polypeptides (ELPs) and could be easily and cheaply prepared.
32820167	6	29	gly	O-glycosites	961:972	arg2	O-glycosites			O-glycosites						O-glycosites	Our study highlights the importance of O-glycosylation in the biology of peptide hormones, and our map of O-glycosites in this large class of biomolecules serves as a discovery platform for an important class of molecules with potential opportunities for drug designs.
36565355	2	49	gly	domain	234:239	arg1	36 tandem epidermal growth factor-like (EGF) repeats			domain	36 tandem epidermal growth factor-like (EGF) repeats					domain	The receptor contains an extracellular domain (ECD) with 36 tandem epidermal growth factor-like (EGF) repeats.
36478308	6	11	gly	glycopeptides	848:860	arg2	The glycopeptides			The glycopeptides						glycopeptides	The glycopeptides were then analysed using a Waters Q-TOF while using a data dependent acquisition (DDA) mass spectrometry experiment.
36598201	6	43	gly	glycosylation	820:832	arg2	both glycosylation sites			both glycosylation sites						sites	However, a recombinant virus that lost both glycosylation sites (glycosylation-defective virus) showed attenuated replication in cultured cell lines.
36192063	9	12	gly	α2,6-sialylation	1761:1776	arg1	Intact N-glycoproteins	Intact N-glycoproteins				Fterm		N-glycoproteins			Intact N-glycoproteins with α2,6-sialylation were associated with coronavirus disease-(COVID)-19.
36192063	9	113	gly	N-glycoproteins	1740:1754	arg1	Intact N-glycoproteins	N-glycoproteins			α2,6-sialylation	Fterm		N-glycoproteins			Intact N-glycoproteins with α2,6-sialylation were associated with coronavirus disease-(COVID)-19.
36698045	13	64	gly	glycosite	1778:1786	arg2	glycosite			glycosite						glycosite	Moreover, all of the analyses were performed on three consecutive manufactured batches and the glycosylation results on both glycosite and glycans showed good batch-to-batch consistency.
37316505	4	28	part_of	protein	675:681	arg1	the stalk region	PD-1 protein		the stalk region		OGER	Site	PD-1 protein	Q15116	region	In this study, we identified the previously undescribed modification sites of O-linked glycan on the stalk region of PD-1 protein using O-protease digestion coupling with intact mass analysis.
36370046	7	12	part_of	protein	1029:1035	arg1	the spike protein receptor-binding domain	spike protein		the spike protein receptor-binding domain		PUBTATOR	Site	spike protein	43740568	domain	Herein, we used designed combinations of glycoengineering enzymes to simplify and control the glycosylation profile of the spike protein receptor-binding domain (RBD).
36370046	7	58	part_of	receptor-binding	1037:1052	arg1	the spike protein receptor-binding domain	receptor		the spike protein receptor-binding domain		Fterm	Site	receptor		domain	Herein, we used designed combinations of glycoengineering enzymes to simplify and control the glycosylation profile of the spike protein receptor-binding domain (RBD).
36370046	7	61	part_of	spike	1023:1027	arg1	the spike protein receptor-binding domain	spike protein		the spike protein receptor-binding domain		PUBTATOR	Site	spike protein	43740568	domain	Herein, we used designed combinations of glycoengineering enzymes to simplify and control the glycosylation profile of the spike protein receptor-binding domain (RBD).
35247653	4	55	gly	sialylated	748:757	arg1	low sialylated glycans				low sialylated glycans						A superior amount of low sialylated glycans and less branched structures were detected in NeuroEPO plus compare to other commercial rhEPOs.
35495330	3	60	gly	dimannosylated	944:957	arg1	dimannosylated peptide			dimannosylated peptide						peptide	Cys-Cys mutagenesis with ion-pairing amino-acids glutamic acid and arginine was calculated by in silico structure-based protein design and allowed for recognizing dimannose and dimannosylated peptide binding to low-affinity binding sites (K D ≈ 11 μM for one C58-C73 bond, and binding to dimannosylated peptide).
37005509	1	31	part_of	sp3-hybridized	189:202	arg1	the sp3-hybridized C2 position	sp3		the sp3-hybridized C2 position		OGER	Site	sp3	Q02447	position	Glycoconjugate analogues in which the sp3-hybridized C2 position of the carbohydrate structure (normally bearing a hydroxyl group) is converted to a compact sp2-hybridized exo-methylene group are expected to have unique biological activities.
36036581	4	18	gly	glycosites	812:821	arg2	20 glycosites			20 glycosites						glycosites	In addition to the common N-glycans as detected in previous studies, many uncommon glycosylation structures such as LacdiNAc structures, Lewis structures, Mannose 6-phosphate (M6P) residues, and bisected core structures were unambiguously mapped at a total of 20 glycosites in the S protein trimer and protomer.
36838941	1	53	gly	used	226:229	arg2	bio-based hydrogel composites			bio-based hydrogel composites						composites	In this work, bio-based hydrogel composites of xanthan gum and cellulose fibers were developed to be used both as soil conditioners and topsoil covers, to promote plant growth and forest protection.
32149134	1	16	gly	glycoprotein	145:156	arg1	a type I transmembrane glycoprotein	a type I transmembrane glycoprotein				Fterm		glycoprotein			PURPOSE CD147, also known as BSG, is a type I transmembrane glycoprotein that belonged to immunoglobulin superfamily.
32149134	2	29	gly	glycosylated	231:242	arg1	an N-linked glycosylated protein	an N-linked glycosylated protein				Fterm		protein			Mature CD147 is an N-linked glycosylated protein and exists on the transmembrane and as soluble forms in tumors.
35104505	9	13	gly	glycoprotein	1701:1712	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			Our findings suggest that the N domain of GnT-V plays a critical role in the recognition of glycoprotein substrates, providing new insights into the mechanism of substrate-selective biosynthesis of N-glycans.
32130226	5	11	gly	glycosylated	947:958	arg1	an OST-dependent glycosylated protein	an OST-dependent glycosylated protein				Fterm		protein			We identified vitellogenin VIT-6 as an OST-dependent glycosylated protein, critical for maintaining survival on PA14.
35481895	5	29	gly	identified	908:917	arg1	a trisaccharide α-GlcNAc-4-β-GlcNAc3NGaAN-4-β-Glc-Asn			a trisaccharide α-GlcNAc-4-β-GlcNAc3NGaAN-4-β-Glc-Asn						Asn	Two N-glycans were identified by NMR and MS analysis: a trisaccharide α-GlcNAc-4-β-GlcNAc3NGaAN-4-β-Glc-Asn where the second residue is 2-N-acetyl, 3-N-glyceryl-glucosamide and a disaccharide β-GlcNAc3NAcAN-4-β-Glc-Asn, where the terminal residue is 2,3 di-N-acetyl-glucosamide.
35941485	2	25	part_of	CS/DS	462:466	arg1	the composition	CS		the composition		PUBTATOR	Site	CS	1431	position	Among GAGs, chondroitin sulfate (CS) and dermatan sulfate (DS) play an essential role at the ECM level; however, the composition of the hybrid CS/DS as well as the distribution of the sulfate groups along the chain were also shown to influence biological activities in brain.
35311852	9	87	gly	glycoproteins	1365:1377	arg1	bovine fetuin	bovine fetuin				Fterm		fetuin			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	87	gly	glycoproteins	1365:1377	arg1	model glycoproteins	model glycoproteins				Fterm		glycoproteins			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	97	gly	derived	1346:1352	arg1	bovine fetuin AND N- and O-glycan isomers	bovine fetuin			N- and O-glycan isomers	Fterm		fetuin			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
35311852	9	97	gly	derived	1346:1352	arg1	model glycoproteins AND N- and O-glycan isomers	model glycoproteins			N- and O-glycan isomers	Fterm		glycoproteins			Then, the optimal separation strategy was applied to achieve separations of N- and O-glycan isomers derived from model glycoproteins, including bovine fetuin, ribonuclease B and κ-casein.
36121379	8	48	gly	sialylated	1233:1242	arg1	highly sialylated multi-antennary glycans				highly sialylated multi-antennary glycans						HILIC separates the crude mixture of highly sialylated multi-antennary glycans, MS provides information on glycan composition, and IMS is used to distinguish and quantify α2,6- and α2,3-linked sialic acid isomers based on characteristic fragments.
37336068	9	70	gly	glycosylated	1657:1668	arg1	β-1,4-GlcNAc glycosylated WTAs				β-1,4-GlcNAc glycosylated WTAs						This study demonstrated that β-1,4-GlcNAc glycosylated WTAs are promising antigens for further development against MRSA.
35604098	2	23	gly	asparagine-linked	435:451	arg1	asparagine-linked (N-) glycans			asparagine	asparagine-linked (N-) glycans					asparagine	Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine-linked (N-) glycans in the organelles.
35604098	2	44	gly	glycoproteins	314:326	arg1	homogeneous glycoproteins	homogeneous glycoproteins				Fterm		glycoproteins			Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine-linked (N-) glycans in the organelles.
35594729	0	44	gly	oxidase	41:47	arg1	N-linked glycans	oxidase			N-linked glycans	Fterm		oxidase			Effects of N-linked glycans of bilirubin oxidase on direct electron transfer-type bioelectrocatalysis.
33078708	2	36	part_of	Ca2+	211:214	arg1	Flagellar Ca2+ signaling nanodomains	Flagellar Ca2		Flagellar Ca2+ signaling nanodomains		OGER	Site	Flagellar Ca2	P00918	nanodomains	Flagellar Ca2+ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration in the female tract, implicating CatSper-dependent mechanisms in sperm selection.
33078708	2	39	part_of	Flagellar	201:209	arg1	Flagellar Ca2+ signaling nanodomains	Flagellar Ca2		Flagellar Ca2+ signaling nanodomains		OGER	Site	Flagellar Ca2	P00918	nanodomains	Flagellar Ca2+ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration in the female tract, implicating CatSper-dependent mechanisms in sperm selection.
36121379	7	6	gly	released	1135:1142	arg2	human alpha-1-acid glycoprotein AND the glycan structures	human alpha-1-acid glycoprotein			the glycan structures	Fterm		glycoprotein			Here, we close this gap and combine hydrophilic interaction liquid chromatography (HILIC) with IM-MS to analyze the glycan structures released from human alpha-1-acid glycoprotein (hAGP).
36121379	7	57	gly	glycoprotein	1168:1179	arg1	human alpha-1-acid glycoprotein	human alpha-1-acid glycoprotein				Fterm		glycoprotein			Here, we close this gap and combine hydrophilic interaction liquid chromatography (HILIC) with IM-MS to analyze the glycan structures released from human alpha-1-acid glycoprotein (hAGP).
37310804	2	74	part_of	ZPS	500:502	arg1	well-defined ZPS fragments	ZPS		well-defined ZPS fragments		Cterm	Site	ZPS		fragments	It remains enigmatic, however, how these polysaccharides bind to these receptors, and to understand the structural features responsible for this "peptide-like" behavior, well-defined ZPS fragments are required in sufficient quantity and quality.
36830744	5	48	part_of	IgG	1016:1018	arg1	IgG N-glycome composition	IgG		IgG N-glycome composition		Cterm	Site	IgG		position	Sex-stratified analysis of 316 CAD- and 156 CAD+ cases revealed differences in IgG N-glycome composition.
36809652	1	85	gly	O-glycoprotein	209:222	arg1	a highly polymorphic O-glycoprotein	a highly polymorphic O-glycoprotein				Fterm		O-glycoprotein			Apolipoprotein(a) [apo(a)] is a highly polymorphic O-glycoprotein circulating in human plasma as lipoprotein(a) [Lp(a)].
34960798	9	117	gly	glycoprotein	1601:1612	arg1	surface glycoprotein	surface glycoprotein				Fterm		glycoprotein			In contrast, aa sequences of surface glycoprotein exhibited higher variability confirming type-specific variation in the SU5 epitope.
36990248	8	10	part_of	T-synthase	1373:1382	arg1	a specific functional domain	T-synthase		a specific functional domain		Fterm	Site	T-synthase		domain	Additionally, a specific functional domain of T-synthase, called the stem domain, was found to be essential for activity and is presumed to be needed for dimer formation and galactosyltransferase activity.
37129482	7	25	gly	glycoproteins	1150:1162	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In addition to glycan structures, key information, such as glycan-related genes, relevant diseases, glycoproteins, and pathways were integrated and cross-linked with one another.
35943155	13	151	part_of	epitopes	2241:2248	arg1	cell surface glycoconjugate receptors	receptors		epitopes		Fterm	Site	receptors		epitopes	IMPORTANCE α-l-Fucosyl residues are frequently present in many relevant glycans, such as human milk oligosaccharides (HMOs), histo-blood group antigens (HBGAs), and epitopes on cell surface glycoconjugate receptors.
37219590	1	26	gly	glycoprotein	168:179	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			The oligosaccharides in N-glycosylation provide key structural and functional contributions to a glycoprotein.
34695439	5	87	gly	glycosylation	799:811	arg1	a protein	a protein				Fterm		protein			We generated B3GLCT knockout (KO) RPE cells and analyzed glycosylation and secretion of thrombospondin 1 (TSP1), a protein involved in cellular processes highly relevant to AMD.
34452516	5	72	gly	glycoprotein	1006:1017	arg1	both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis				both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis						Mutation of the LH6 initiating glycosyltransferase homolog, PglC, necessary for both O-linked glycoprotein and capsular polysaccharide (CPS) biosynthesis, prevented infection by the lytic podovirus CAP1, while mutation of the pilin protein, PilA, prevented infection by CAP3, representing the lytic cystoviruses.
35320529	8	34	part_of	receptor	1447:1454	arg1	specific glycosylation sites	receptor		specific glycosylation sites		Fterm	Site	receptor		sites	Thus, the protocol described in this section can be utilized to identify galectin ligands at specific glycosylation sites of endogenous membrane receptor from circulating primary human lymphocytes.
37162352	5	13	gly	sequence	716:723	arg1	the sequence Hex-(Me-dHex)-Me-HexA-Pent-HexA-Me-HexNAcA				the sequence Hex-(Me-dHex)-Me-HexA-Pent-HexA-Me-HexNAcA						The structure of the major glycan was found to be a hexasaccharide with the sequence Hex-(Me-dHex)-Me-HexA-Pent-HexA-Me-HexNAcA.
36809652	0	93	gly	structures	9:18	arg1	apo(a) subunit	subunit			structures	Fterm		subunit			O-glycan structures in apo(a) subunit of human lipoprotein(a) suppresses the pro-angiogenic activity of galectin-1 on human umbilical vein endothelial cells.
35276597	5	46	gly	N-glycosylation	1207:1221	arg1	rMIR				rMIR						The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone.
35276597	5	46	gly	N-glycosylation	1207:1221	arg1	nMIR				nMIR						The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone.
35276597	5	67	gly	peptide	1264:1270	arg1	the peptide backbone				the peptide backbone						The detailed peptide mapping and N-glycosylation analysis of MIRs provided evidence that MIRs have variants, which were derived mainly from the differences in the N-glycan structure in nMIR and the N-glycosylation in rMIR and not from the cleavage of the peptide backbone.
35987426	14	0	gly	glycoproteins	2206:2218	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			CONCLUSIONS This study showed that the glycan pattern becomes more complex from the basal to surface layer of the urothelium and that surface cells could modify the composition of urine via the secretion of glycoproteins.
32592613	2	60	part_of	protein-3	363:371	arg1	The C-terminal domain	protein-3		The C-terminal domain		Fterm	Site	protein-3		domain	The C-terminal domain of insulin-like growth factor-binding protein-3 (IGFBP-3) is known to contain an 18-basic amino acid motif capable of interacting with either humanin (HN) or hyaluronan (HA).
32592613	2	46	part_of	contain	395:401	arg1	The C-terminal domain AND an 18-basic amino acid motif	protein-3		motif		Fterm	Site	protein-3		motif	The C-terminal domain of insulin-like growth factor-binding protein-3 (IGFBP-3) is known to contain an 18-basic amino acid motif capable of interacting with either humanin (HN) or hyaluronan (HA).
33789105	5	33	part_of	Notch	799:803	arg1	Notch TM/ICD fragment	Notch		Notch TM/ICD fragment		PUBTATOR	Site	Notch	4851	fragment	Mechanistic studies show that Ogt directly interacts with Notch1 and catalyzes the O-GlcNAc modification of Notch TM/ICD fragment.
33789105	5	49	part_of	TM/ICD	805:810	arg1	Notch TM/ICD fragment	ICD		Notch TM/ICD fragment		PUBTATOR	Site	ICD	79158	fragment	Mechanistic studies show that Ogt directly interacts with Notch1 and catalyzes the O-GlcNAc modification of Notch TM/ICD fragment.
36758646	2	32	gly	glycoproteins	384:396	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In insects, α-1,6-fucosyltransferase (FucT6) is an important enzyme in the glycosylation pathway, modifying the core structure of N-glycans on glycoproteins with the addition of a fucose residue.
35065968	7	16	gly	carrying	1502:1509	arg1	peptides AND a type A glycan modification			peptides	a type A glycan modification					peptides	In conclusion, we show that a common enrichment strategy results in reliable identification of peptides carrying a type A glycan modification, and that the results obtained can be used to advance models about its biosynthesis.
36377874	10	123	gly	glycosylated	1675:1686	arg1	The glycan loop deletion mutants				The glycan loop deletion mutants						The glycan loop deletion mutants are not glycosylated, which are attenuated in adult A129 mouse model and reduced oral infections in mosquitoes.
35247653	7	6	gly	glycoprotein	1301:1312	arg1	intact glycoprotein	intact glycoprotein				Fterm		glycoprotein			The proposed glycomic and intact glycoprotein methods provide a robust and reliable analytical platform for NeuroEPO plus characterization and for its future implementation as biopharmaceutical in neurodegenerative diseases.
36585837	6	9	gly	glycopeptides	815:827	arg2	Purified glycopeptides			Purified glycopeptides						glycopeptides	Purified glycopeptides were analysed by label-free liquid chromatography tandem mass-spectrometry (LC-MS/MS).
37069671	9	31	gly	composition	1404:1414	arg1	particular abundant storage polysaccharides			position	particular abundant storage polysaccharides					position	CONCLUSIONS We show that the amounts and composition of dissolved polysaccharides, in particular abundant storage polysaccharides, have a pronounced influence on the composition of abundant bacterioplankton members during phytoplankton blooms, some of which compete for similar polysaccharide niches.
36463509	4	28	gly	glycosylation	626:638	arg1	an Fc-fusion protein	an Fc-fusion protein				Fterm		protein			In this study, the biosimilars of a therapeutic monoclonal antibody and an Fc-fusion protein with moderate and heavy glycosylation, respectively, were employed as representative biopharmaceuticals for released glycan analysis using liquid chromatography-tandem mass spectrometry instead of conventional mass spectrometry-based analysis.
36210615	2	89	gly	glycoprotein	346:357	arg1	their glycoprotein content	their glycoprotein content				Fterm		glycoprotein			Studies by our group have shown that Bothrops venoms are distinctly defined by their glycoprotein content, and that most hybrid/complex N-glycans identified in these venoms contain sialic acid.
36669592	6	6	gly	sialylated	1039:1048	arg1	sialylated O-glycan structures				sialylated O-glycan structures						It was found that the fully differentiated butyrate-stimulated cells are characterized by a higher expression of sialylated O-glycan structures, whereas fucosylation is downregulated with differentiation.
33206215	4	45	part_of	ECM	705:707	arg1	ECM peptides	ECM		ECM peptides		OGER	Site	ECM	Q13201	peptides	Conventional peptide analysis via trypsin inefficiently captures ECM peptides due to their low abundance, intra- and intermolecular cross-linking, and PTMs.
35662639	4	52	gly	glycoproteins	1218:1230	arg1	sialic acid-containing glycoproteins				sialic acid-containing glycoproteins						To further investigate the mechanisms involved in the generation of different venoms by related snakes, here the glycoproteomes of nine Bothrops venoms (Bothrops atrox, B. cotiara, Bothrops erythromelas, Bothrops fonsecai, B. insularis, Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni and Bothrops neuwiedi) were comparatively analyzed by enrichment with three lectins of different specificities, recognizing bisecting N-acetylglucosamine- and sialic acid-containing glycoproteins, and mass spectrometry.
36136114	4	33	gly	glycosylation	568:580	arg1	the active peptide			peptide						peptide	Fc-fusion proteins can be modified by complex glycosylation on the active peptide, the fragment crystallizable (Fc) domain, and the linker peptides.
36136114	4	33	gly	glycosylation	568:580	arg1	the linker peptides			peptides						peptides	Fc-fusion proteins can be modified by complex glycosylation on the active peptide, the fragment crystallizable (Fc) domain, and the linker peptides.
36136114	4	33	gly	glycosylation	568:580	arg1	the fragment crystallizable (Fc) domain			domain						domain	Fc-fusion proteins can be modified by complex glycosylation on the active peptide, the fragment crystallizable (Fc) domain, and the linker peptides.
35316032	3	28	gly	deglycosylation	820:834	arg1	an M6P-glycan				an M6P-glycan						Using trastuzumab and cetuximab as model antibodies, we showed that the wild-type endoglycosidase S (Endo-S) could efficiently perform the antibody deglycosylation and simultaneous transfer of an M6P-glycan from a synthetic M6P-glycan oxazoline to the deglycosylated antibody in a one-pot manner, giving structurally well-defined antibody-M6P glycan conjugates.
35092134	11	13	gly	MET	1623:1625	arg1	N-glycans			MET	N-glycans					MET	The overall findings suggest that N-glycans of MET affect the status and the function of the receptor in a site-specific manner.
35943155	18	82	gly	glycoproteins	3061:3073	arg1	naturally occurring glycoproteins	naturally occurring glycoproteins				Fterm		glycoproteins			These enzymes showed different substrate specificities toward HMOs, HBGAs, naturally occurring glycoproteins, and neoglycoproteins.
35943155	18	157	gly	neoglycoproteins	3080:3095	arg1	neoglycoproteins	neoglycoproteins				Fterm		neoglycoproteins			These enzymes showed different substrate specificities toward HMOs, HBGAs, naturally occurring glycoproteins, and neoglycoproteins.
34379775	2	35	gly	O-glycosylated	425:438	arg1	O-glycosylated proteins	O-glycosylated proteins				Fterm		proteins			The function of cervical mucus is critically linked to its rheological properties that are in turn dictated by O-glycosylated proteins, called mucins.
33073321	0	97	gly	glycopeptides	32:44	arg2	urinary N-linked glycopeptides			urinary N-linked glycopeptides						glycopeptides	Recognition of urinary N-linked glycopeptides in kidney cancer patients by hydrophilic carbohydrate functionalized magnetic metal organic framework combined with LC-MS/MS.
37294165	8	15	gly	glycoprotein	1470:1481	arg1	a target glycoprotein	a target glycoprotein				Fterm		glycoprotein			This synthetic concept enables us to repetitively synthesize a target glycoprotein easily.
35307819	8	9	part_of	IgG	1348:1350	arg1	IgG Fc glycome composition	IgG		IgG Fc glycome composition		Cterm	Site	IgG	668542	position	This study brings to light a novel blood biomarker based on IgG Fc glycome composition with capacity to stratify patients at diagnosis.
34399822	2	103	gly	kinase	415:420	arg1	O-GlcNAcylation	kinase 3			O-GlcNAcylation	Fterm		kinase 3			The O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) of necroptotic signal molecule receptor-interacting serine-threonine kinase 3 (RIPK3) was reported to exert a protective effect in gut inflammation.
36740532	7	28	gly	O-glycosites	1140:1151	arg2	62 unique O-glycosites			62 unique O-glycosites						O-glycosites	In the analysis of the recombinant protein, we identified 62 unique O-glycosites.
36373229	7	6	gly	O-glycopeptide	1567:1580	arg2	O-glycopeptide			O-glycopeptide						O-glycopeptide	These putative motifs can be further used to define new protease cleavage settings that lower search times and improve O-glycopeptide identifications.
36373229	7	41	gly	used	1485:1488	arg2	These putative motifs			These putative motifs						motifs	These putative motifs can be further used to define new protease cleavage settings that lower search times and improve O-glycopeptide identifications.
34939082	4	39	gly	glycopeptides	561:573	arg2	glycopeptides			glycopeptides						glycopeptides	We illustrate this method by showing visual representations of the glycans and glycopeptides from a variety of species.
36018613	13	71	gly	glycopeptide	1627:1638	arg2	glycopeptide sequence identification			glycopeptide sequence identification						glycopeptide	If general, the change to a furanose m/z 204 product ion structure fundamentally alters the ion population available for MS3 dissociation and glycopeptide sequence identification.
35802832	5	43	gly	disialylated	986:997	arg1	disialylated O-glycans				disialylated O-glycans						Bovine glycomacropeptide glycosylation promoted the immune activity of RAW264.7 cells, which may be linked to a higher content of disialylated O-glycans.
32921312	3	81	part_of	HDL	614:616	arg1	The HDL composition	HDL		The HDL composition		OGER	Site	HDL	Q9UNE0	position,	The HDL composition, proteome, chemical modification and functionality were analyzed in nondialysis subjects with DKD categorized according to the estimated glomerular filtration rate (eGFR) and urinary albumin excretion rate (AER).
35320529	7	0	gly	N-glycosylation	1124:1138	arg2	five N-glycosylation sites			five N-glycosylation sites						sites	NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for endogenous galectins.
35320529	7	62	gly	modified	1177:1184	arg3	the five sites AND N-glycans			the five sites	N-glycans					sites	NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for endogenous galectins.
35320529	7	36	gly	glycosylated	1103:1114	arg2	five N-glycosylation sites	CD16a		sites		PUBTATOR		CD16a	2214	sites	NK cell CD16a is glycosylated at five N-glycosylation sites, and two of the five sites are modified, almost exclusively, by N-glycans with multiple LacNAc repeats which can serve as ligands for endogenous galectins.
35854001	1	44	gly	glycoprotein	231:242	arg1	glycoprotein functions	glycoprotein functions				Fterm		glycoprotein	269181		N-Glycosylation is a common post-translational modification, and the number of GlcNAc branches in N-glycans impacts glycoprotein functions.
32602701	4	28	gly	glycosylation	569:581	arg2	all four potential Asn glycosylation sites			all four potential Asn glycosylation sites						sites	Abolishing N-linked glycosylation by tunicamycin, glucosamine supplementation, or glutamine substitutions of all four potential Asn glycosylation sites blocked myonectin secretion.
32602701	4	81	gly	glycosylation	457:469	arg1	all four potential Asn glycosylation sites			all four potential Asn glycosylation sites						sites	Abolishing N-linked glycosylation by tunicamycin, glucosamine supplementation, or glutamine substitutions of all four potential Asn glycosylation sites blocked myonectin secretion.
36399685	7	44	gly	N-glycoproteins	1195:1209	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			We anticipate that our method will enable selection and evolution of N-glycoproteins with advantageous biological and biophysical properties.
34939126	8	25	gly	glycoproteins	1341:1353	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This study expands structural knowledge on fungal O-glycosylation and presents a ready-to-use enzymatic approach for controlled O-glycan engineering in glycoproteins expressed in filamentous fungi.
36192063	10	73	gly	N-glycoproteins	2047:2061	arg1	intact N-glycoproteins	intact N-glycoproteins				Fterm		N-glycoproteins			Additionally, many types of N-glycosylation including terminal N-galactosylation, core and/or branch fucosylation, α2,6-sialylation and terminal bisecting N-acetylglucosamine were identified and quantified in intact N-glycoproteins from immunoglobulin family.
36985724	3	34	gly	glycoproteins	504:516	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycoprotein in vitro N-glycan engineering using purified recombinant enzymes is an attractive strategy to produce glycoproteins with homogeneous N-glycoforms to elucidate the specific functions of N-glycans and develop better glycoprotein therapeutics.
36985724	3	41	gly	glycoprotein	616:627	arg1	better glycoprotein therapeutics	better glycoprotein therapeutics				Fterm		glycoprotein			Glycoprotein in vitro N-glycan engineering using purified recombinant enzymes is an attractive strategy to produce glycoproteins with homogeneous N-glycoforms to elucidate the specific functions of N-glycans and develop better glycoprotein therapeutics.
37156312	2	39	gly	O-glycosites	402:413	arg2	O-glycosites			O-glycosites						O-glycosites	Unlike N-glycosylation, the O-glycosylation has nonspecific sequence features and nonstable glycan core structure, which makes identification of O-glycosites more challenging either by experimental or computational methods.
36435935	3	30	gly	glycoprotein	493:504	arg1	glycoprotein biosynthesis	glycoprotein biosynthesis				Fterm		glycoprotein	281499		Precise recognition of the fully assembled glycan by OST is essential for the subsequent quality control steps of glycoprotein biosynthesis.
31941975	5	13	gly	N-glycopeptides	890:904	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Training and test sets of more than 800 MS/MS spectra of N-glycopeptides from the immunoglobulin gamma and alpha 1-acid-glycoprotein standards were selected for classification of the fucosylation types using supervised learning models.
31941975	5	75	gly	1-acid-glycoprotein	946:964	arg1	the immunoglobulin gamma and alpha 1-acid-glycoprotein standards	the immunoglobulin gamma and alpha 1-acid-glycoprotein standards				Fterm		1-acid-glycoprotein			Training and test sets of more than 800 MS/MS spectra of N-glycopeptides from the immunoglobulin gamma and alpha 1-acid-glycoprotein standards were selected for classification of the fucosylation types using supervised learning models.
34712242	4	16	gly	sialylated	798:807	arg1	sialylated glycan species				sialylated glycan species						While the position of the Fc glycan is conserved in macaques, differences in the frequency of glycoforms and the use of an alternate monosaccharide in sialylated glycan species add a degree of uncertainty to the testing of glycoengineered human antibodies in rhesus macaques.
36288283	4	15	gly	glycoprotein	622:633	arg1	the LASV glycoprotein complex	the LASV glycoprotein complex				Fterm		glycoprotein			Here, we describe biochemical analyses and high-resolution cryo-electron microscopy structures of a therapeutic cocktail of three broadly protective antibodies that target the LASV glycoprotein complex (GPC), previously identified from survivors of multiple LASV infections.
32357974	6	84	gly	N-glycosylated	1191:1204	arg1	nascently N-glycosylated polypeptides			nascently N-glycosylated polypeptides						polypeptides	This finding, demonstrated previously for a handful of deamidated T cell epitopes, implicates a more global role for the retrograde transport of nascently N-glycosylated polypeptides from the ER and their subsequent degradation within the cytosol to form HLA-ligand precursors.
36244450	6	7	gly	glycosylation	939:951	arg2	glycosylation site characterization			glycosylation site characterization						site	We conducted site-specific N- and O-glycosylation analysis and glycosylation site characterization using mass spectrometry by which several novel O-glycosylation sites were identified in mouse MR and confirmed in human full-length MR. This information guided experiments evaluating the receptor functionality by glycan microarray analysis in combination with glycan-modifying enzymes.
36244450	6	88	gly	O-glycosylation	1022:1036	arg2	several novel O-glycosylation sites			several novel O-glycosylation sites						sites	We conducted site-specific N- and O-glycosylation analysis and glycosylation site characterization using mass spectrometry by which several novel O-glycosylation sites were identified in mouse MR and confirmed in human full-length MR. This information guided experiments evaluating the receptor functionality by glycan microarray analysis in combination with glycan-modifying enzymes.
36096000	1	48	gly	glycosylation	117:129	arg1	egg white proteins	egg white proteins				Fterm		proteins			The glycosylation of egg white proteins (EWP) with maltodextrin (MD) was investigated by monitoring their gel properties and protein structure.
32839225	5	56	gly	sialylated	902:911	arg1	their sialylated oligosaccharides				their sialylated oligosaccharides						Using tandem MS coupled with liquid chromatography (LC-MS/MS), we found 142 nonreductant peptides, with 9 of them showing potential modifications on their sialylated oligosaccharides during capacitation.
36990248	4	17	gly	attached	816:823	arg1	secreted proteins AND the O-glycan	secreted proteins			the O-glycan	Fterm		proteins			We found GalNAc or GlcNAc monosaccharide and core 1 disaccharide (Galβ1-3-GalNAcα1-Ser/Thr) were major components of the O-glycan attached to secreted proteins produced in silkworms.
35447118	4	16	gly	fucosylation	871:882	arg1	typical lung-type N-glycans				typical lung-type N-glycans						We found that the N-glycans of SCLC-sEVs were characterized by the presence of structural units also found in the brain N-glycome, while NSCLC-sEVs were dominated by typical lung-type N-glycans with NSCLC-associated core fucosylation.
35882856	4	36	gly	fucosylated	657:667	arg1	fucosylated glycans				fucosylated glycans						Interestingly, TMPRSS2 and fucosylated glycans can mediate the binding/entry of TcsH independently, thus serving as redundant receptors.
33343565	3	24	gly	epitope	758:764	arg1	a self-binding epitope tag				a self-binding epitope tag						Complement protein C1q binding of the IgG fusions was enhanced by: 1) antigen fusion to the IgG N-terminus; 2) removal of the IgG light chain or Fab regions; 3) addition of hexamer-inducing mutations in the IgG Fc; 4) adding a self-binding epitope tag to create recombinant immune complexes (RIC); or 5) producing IgG fusions in plants that lack plant-specific β1,2-linked xylose and α1,3-linked fucose N-linked glycans.
35211008	11	97	gly	N-glycosylation	1915:1929	arg1	β1 adrenergic receptors	β1 adrenergic receptors				Fterm		receptors			The N-glycosylation of β1 adrenergic receptors decreased after continuous isoproterenol stimulation, while stachytine hydrochloride can increase the N-glycosylation of β1AR in the heart of mice with isoproterenol-induced heart failure.
36838558	1	31	gly	protein	255:261	arg1	the GalNAc-attached form	protein			the GalNAc-attached form	Fterm		protein			O-linked α-N-acetylgalactosamine (α-GalNAc) in the Gc protein is essential for macrophage activation; thus, the GalNAc-attached form of Gc protein is called Gc macrophage activating factor (GcMAF).
35193013	1	1	gly	glycoprotein	197:208	arg1	serum glycoprotein N-glycans				serum glycoprotein N-glycans						Characterization of serum glycoprotein N-glycans with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in positive-ion mode needs a derivatization step to stabilize and neutralize the negative charge on sialic acids.
36565991	3	25	gly	glycosylation	357:369	arg2	glycosylation attachment sites			glycosylation attachment sites						sites	One class of mutation impacts glycosylation attachment sites, which have the capacity to influence the antigenic structure beyond the immediate site of attachment.
35569685	1	38	gly	origin	302:307	arg1	three polysaccharide gums	origin			three polysaccharide gums	Fterm		origin			A facile, green synthesis methodology to obtain zinc oxide nanoparticles using three polysaccharide gums (Acacia gum, Guar gum and Xanthan gum) of biological origin was developed.
33073321	6	61	gly	glycopeptides	1436:1448	arg2	the identified glycopeptides			the identified glycopeptides						glycopeptides	Further gene ontology analysis of molecular function and biological process revealed that 13 original glycoproteins of the identified glycopeptides from urine of patients significantly participate in diverse cancer-associated events, including collagen binding, immunoglobulin receptor binding, antigen binding, and complement activation process.
33073321	6	67	gly	glycoproteins	1404:1416	arg1	13 original glycoproteins	13 original glycoproteins				Fterm		glycoproteins			Further gene ontology analysis of molecular function and biological process revealed that 13 original glycoproteins of the identified glycopeptides from urine of patients significantly participate in diverse cancer-associated events, including collagen binding, immunoglobulin receptor binding, antigen binding, and complement activation process.
35802124	3	20	gly	region	685:690	arg1	the tissue				the tissue						Unstandardized and uncontrolled incubation steps often cause significant delocalization of released N-glycans, resulting in the inability to link given N-glycan composition to a specific microanatomical region in the tissue.
37287875	2	37	gly	glycopeptide	219:230	arg2	the intact glycopeptide level			the intact glycopeptide level						glycopeptide	Glycosylation has been increasingly analyzed at the intact glycopeptide level using mass spectrometry to study site-specific glycosylation changes under different physiological and pathological conditions.
36018613	11	64	gly	fragment	1269:1276	arg1	the m/z 204, C8H14N1O5+, glycan fragment population				the m/z 204, C8H14N1O5+, glycan fragment population						IR action spectra for the m/z 204, C8H14N1O5+, glycan fragment population support this prediction.
34960798	7	61	part_of	SRLVs	1319:1323	arg1	gag and env SRLVs sequences	env SRLVs		gag and env SRLVs sequences		PUBTATOR	Site	env SRLVs	100616444	sequences	Furthermore, three putative recombination events were identified within gag and env SRLVs sequences derived from three sheep.
34960798	7	130	part_of	env	1315:1317	arg1	gag and env SRLVs sequences	env SRLVs		gag and env SRLVs sequences		PUBTATOR	Site	env SRLVs	100616444	sequences	Furthermore, three putative recombination events were identified within gag and env SRLVs sequences derived from three sheep.
34960798	7	135	part_of	gag	1307:1309	arg1	gag and env SRLVs sequences	gag		gag and env SRLVs sequences		PUBTATOR	Site	gag	155030	sequences	Furthermore, three putative recombination events were identified within gag and env SRLVs sequences derived from three sheep.
33006605	7	45	part_of	N-linked	1113:1120	arg1	N-linked glycosylation sites	N		N-linked glycosylation sites		PUBTATOR	Site	N	43740575	sites	Moreover, many mutations in N-linked glycosylation sites can increase the stability of the S protein.
34985300	12	56	gly	glycosylation	2174:2186	arg2	257 glycosylation sites			257 glycosylation sites						sites	This study has for the first time demonstrated the presence of O-linked glycosylation in this organism by determining the basic structure of the O-glycans and identifying 257 glycosylation sites in 145 proteins.
36493594	1	1	gly	N-glycans	192:200	arg1	glycoproteins	glycoproteins			N-glycans	Fterm		glycoproteins			BACKGROUND N-glycans in glycoproteins can affect physicochemical properties of proteins; however, some reported N-glycan structures are inconsistent depending on the type of glycoprotein or the preparation methods.
36493594	1	11	gly	glycoproteins	205:217	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			BACKGROUND N-glycans in glycoproteins can affect physicochemical properties of proteins; however, some reported N-glycan structures are inconsistent depending on the type of glycoprotein or the preparation methods.
36493594	1	19	gly	glycoprotein	355:366	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			BACKGROUND N-glycans in glycoproteins can affect physicochemical properties of proteins; however, some reported N-glycan structures are inconsistent depending on the type of glycoprotein or the preparation methods.
35373202	4	64	gly	glycosylation	833:845	arg2	the glycosylation site selection			the glycosylation site selection						site	By using MUC1, with four tandem repeat domains as a substrate, we confirmed the glycosylation preferences of different GalNAc-Ts isoforms and highlighted the importance of the lectin domain in the glycosylation site selection after the addition of the first GalNAc residue.
35320529	4	44	gly	composition	666:676	arg1	primary lymphocyte membrane receptors	receptors			composition	Fterm		receptors			However, the site-specific glycan composition on primary lymphocyte membrane receptors in healthy individuals is largely limited.
35995381	7	82	gly	6 N-glycosylation	1078:1094	arg2	6 N-glycosylation sites			6 N-glycosylation sites						sites	It was noticed that 6 N-glycosylation sites contain only high-mannose type glycans, while other regions include complex and hybrid type glycans.
35995381	7	38	gly	contain	1102:1108	arg1	6 N-glycosylation sites AND high-mannose type glycans			6 N-glycosylation sites	high-mannose type glycans					sites	It was noticed that 6 N-glycosylation sites contain only high-mannose type glycans, while other regions include complex and hybrid type glycans.
37348475	6	17	gly	glycosylation	1191:1203	arg1	the AGP precursor	the AGP precursor				Fterm		precursor			These observations indicate that GPI-anchoring is required for the proper transport and glycosylation of the AGP precursor.
34864901	6	20	gly	proteins	1066:1073	arg1	the glycan shielding	proteins			the glycan shielding	Fterm		proteins			Overall, GLYCO provides fundamental insights into the glycan shielding of glycosylated proteins.
34864901	6	47	gly	glycosylated	1053:1064	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			Overall, GLYCO provides fundamental insights into the glycan shielding of glycosylated proteins.
35995381	15	61	gly	glycosylation	2267:2279	arg2	all determined glycosylation sites			all determined glycosylation sites						sites	In addition, N-glycan structures containing core-fucosylation and bisecting types were confirmed for all determined glycosylation sites.
35995381	11	45	gly	N-glycosylation	1648:1662	arg2	the N-glycosylation sites	thyroglobulin		sites		PUBTATOR		thyroglobulin	7038	sites	Therefore, a comprehensive analysis of the N-glycosylation sites of human thyroglobulin is essential to improve our understanding of the function of its N-glycans.
32357974	0	93	part_of	HLA-bound	50:58	arg1	Deamidated HLA-bound Peptides	HLA		Deamidated HLA-bound Peptides		OGER		HLA			Immunopeptidomic Analysis Reveals That Deamidated HLA-bound Peptides Arise Predominantly from Deglycosylated Precursors.
37162352	3	0	gly	O-glycosylation	386:400	arg1	S-layer proteins	S-layer proteins				Fterm		proteins			O-Glycosylation has been reported in several Bacteroidota species, and the O-glycosylation of S-layer proteins in Tannerella forsythia was shown to be important for certain virulence features.
35311852	3	100	gly	microheterogeneity	423:440	arg1	glycan isomeric structures				glycan isomeric structures						However, the separation and identification of glycan isomers has been a challenge for years due to the microheterogeneity of glycan isomeric structures.
37382290	7	31	gly	N-glycopeptide	1223:1236	arg2	synthetic stable isotope-labeled N-glycopeptide standards			synthetic stable isotope-labeled N-glycopeptide standards						N-glycopeptide	Here, we particularly focused on N-glycoproteomics analysis and investigated fragmentation specificity using synthetic stable isotope-labeled N-glycopeptide standards.
35421698	2	45	gly	N-glycopeptides	276:290	arg2	the intact N-glycopeptides			N-glycopeptides	Monosaccharide composition-level characterization					N-glycopeptides	Monosaccharide composition-level characterization at the intact N-glycopeptides has been extensively reported, yet structure-specific study to resolve multiple sequence structures of a single composition is still lacking.
35092134	7	8	gly	MET	1156:1158	arg1	N-glycan-deletion mutants			MET	N-glycan-deletion mutants					MET	To examine the effects of the deletion of N-glycans of MET, we prepared endogenous MET knockout Flp-In CHO cells and transfected them with a series of N-glycan-deletion mutants of MET.
35092134	7	38	gly	MET	1031:1033	arg1	N-glycans			MET	N-glycans					MET	To examine the effects of the deletion of N-glycans of MET, we prepared endogenous MET knockout Flp-In CHO cells and transfected them with a series of N-glycan-deletion mutants of MET.
37216524	10	60	gly	glycoproteins	1564:1576	arg1	GalNAcα1-O-Ser/Thr/Tyr	glycoproteins			GalNAcα1-O-Ser/Thr/Tyr	Fterm		glycoproteins			Although A20D-Cosmc is functional, its decreased expression, though in a cell or tissue-specific manner, causes a large reduction of T-synthase protein and activity, which accordingly leads to expression of varied amounts of pathological Tn-antigen (GalNAcα1-O-Ser/Thr/Tyr) on multiple glycoproteins.
36698045	10	58	gly	19 N-glycosites	1343:1357	arg2	19 N-glycosites			19 N-glycosites						19 N-glycosites	As a result, a total of 19 N-glycosites were identified in the recombinant pike protein at intact N-glycopeptide level.
36698045	10	84	gly	N-glycopeptide	1417:1430	arg2	intact N-glycopeptide level			intact N-glycopeptide level						N-glycopeptide	As a result, a total of 19 N-glycosites were identified in the recombinant pike protein at intact N-glycopeptide level.
36709835	6	11	gly	afucosylated	1021:1032	arg1	afucosylated, high-mannose glycans				afucosylated, high-mannose glycans						While Humira® had the fewest unique glycans, 15, and 11.4 ± 0.8% of afucosylated, high-mannose glycans.
35713525	11	2	part_of	Sur1	1636:1639	arg1	essential luminal domains	Sur1		essential luminal domains		PUBTATOR	Site	Sur1	856050	domains	Collectively, the present study reveals essential luminal domains of Sur1 and Csh1 that dictate substrate specificity and/or the protein stabilities via mannan modification.
35713525	11	34	part_of	Csh1	1645:1648	arg1	essential luminal domains	Csh1		essential luminal domains		PUBTATOR	Site	Csh1	852458	domains	Collectively, the present study reveals essential luminal domains of Sur1 and Csh1 that dictate substrate specificity and/or the protein stabilities via mannan modification.
36758646	9	54	gly	α-1,6-fucosylation	1363:1380	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Therefore, correct α-1,6-fucosylation of glycoproteins is important for N. lugens during embryonic development and this study provides new insights into the role of N-glycosylation in embryogenesis in insects.
36758646	9	62	gly	glycoproteins	1385:1397	arg1	correct α-1,6-fucosylation	glycoproteins			correct α-1,6-fucosylation	Fterm		glycoproteins			Therefore, correct α-1,6-fucosylation of glycoproteins is important for N. lugens during embryonic development and this study provides new insights into the role of N-glycosylation in embryogenesis in insects.
32592613	7	52	part_of	IGFBP-3	1259:1265	arg1	the basic 18-amino acid residue sequence	IGFBP-3		the basic 18-amino acid residue sequence		OGER	Site	IGFBP-3	P17936	sequence	Binding of IGFBP-3 to either HA or HN was unaffected by glycosylation or reduction of IGFBP-3, suggesting that the basic 18-amino acid residue sequence of IGFBP-3 remains accessible for interaction with either HN or HA upon glycosylation or reduction of the full-length protein.
34726173	0	83	gly	glycosylation	45:57	arg1	staphylococcal adhesive proteins	staphylococcal adhesive proteins				Fterm		proteins			Structural basis for SdgB- and SdgA-mediated glycosylation of staphylococcal adhesive proteins.
36502942	8	10	part_of	COL/CNC	1307:1313	arg1	COL/CF and COL/CNC composites	COL/CNC		COL/CF and COL/CNC composites		OGER	Site	COL/CNC	P32418	composites	Considering the factors of crystallinity, hydrogen bonding, and interfacial tortuosity, COL/CNF composites possessed better mechanical behaviors than that of COL/CF and COL/CNC composites.
37186866	5	1	part_of	IPT	1212:1214	arg1	IPT domains	IPT		IPT domains		OGER	Site	IPT	Q9H3H1	domains	Thus, our study identifies the third protein-specific O-mannosylation pathway in mammals and demonstrates that O-mannosylation of IPT domains serves critical functions during epithelial morphogenesis.
36211377	9	60	part_of	IgG	1464:1466	arg1	their IgG glycome composition	IgG		their IgG glycome composition		Cterm	Site	IgG		position	However, it should be noted that in all five diet groups, there were individuals who prominently altered their IgG glycome composition in either proinflammatory or anti-inflammatory directions.
37354227	6	57	gly	N-glycosylation	1062:1076	arg2	the N-glycosylation sequons			the N-glycosylation sequons							Some of the N-glycosylation sequons in S show differences between SARS-CoV-2 variants in the distribution of glycan forms.
35166741	0	52	gly	glycosylated	37:48	arg1	glycosylated interferon-β-polypeptide			glycosylated interferon-β-polypeptide						interferon-β-polypeptide	Optimizing the Semisynthesis towards glycosylated interferon-β-polypeptide by utilizing bacterial protein expression and chemical modification.
35316032	5	30	gly	glycosylated	1332:1343	arg1	both Fc and Fab domains			both Fc and Fab domains						domains	The chemoenzymatic approach was highly specific for Fc glycan remodeling when both Fc and Fab domains were glycosylated, as exemplified by the selective Fc-glycan remodeling of cetuximab.
34691043	1	45	gly	glycoproteins	176:188	arg1	its surface exposed glycoproteins	its surface exposed glycoproteins				Fterm		glycoproteins			Influenza virus alters glycosylation patterns on its surface exposed glycoproteins to evade host adaptive immune responses.
36534501	1	67	gly	leucine-rich	117:128	arg1	Small leucine-rich proteoglycans			leucine	Small leucine-rich proteoglycans					leucine	Small leucine-rich proteoglycans (SLRPs) are major regulators of extracellular matrix assembly and cell signaling.
31941975	8	44	gly	derived	1464:1470	arg1	54 glycoproteins AND 4 dual-fucosylation types	54 glycoproteins			4 dual-fucosylation types	Fterm		glycoproteins			A total of 82N-glycopeptides, with 54 core-, 24 outer-, and 4 dual-fucosylation types derived from 54 glycoproteins, were commonly classified as the same type in both the DNN and SVM.
31941975	8	62	gly	glycoproteins	1480:1492	arg1	54 glycoproteins	54 glycoproteins				Fterm		glycoproteins			A total of 82N-glycopeptides, with 54 core-, 24 outer-, and 4 dual-fucosylation types derived from 54 glycoproteins, were commonly classified as the same type in both the DNN and SVM.
31941975	8	81	gly	82N-glycopeptides	1389:1405	arg1	4 dual-fucosylation types			82N-glycopeptides	4 dual-fucosylation types					82N-glycopeptides	A total of 82N-glycopeptides, with 54 core-, 24 outer-, and 4 dual-fucosylation types derived from 54 glycoproteins, were commonly classified as the same type in both the DNN and SVM.
35304921	15	50	gly	glycoproteins	2201:2213	arg1	"native" glycoproteins	"native" glycoproteins				Fterm		glycoproteins			Furthermore, the α5 β1 integrin preparation that was analyzed here is from a natural source, which is of importance as there is not a lot of literature in the field about the glycosylation of "native" glycoproteins.
35304921	15	93	gly	glycosylation	2175:2187	arg1	"native" glycoproteins	"native" glycoproteins				Fterm		glycoproteins			Furthermore, the α5 β1 integrin preparation that was analyzed here is from a natural source, which is of importance as there is not a lot of literature in the field about the glycosylation of "native" glycoproteins.
36345209	9	11	gly	glycoproteins	1046:1058	arg1	Specific glycoproteins	Specific glycoproteins				Fterm		glycoproteins			Specific glycoproteins were found to increase in the 3D cell, elucidating the pathways that are affected between the two models.
32790871	7	10	part_of	N-CSP	1103:1107	arg1	the 5D5 N-CSP epitope	CSP		the 5D5 N-CSP epitope		OGER	Site	CSP	Q9H3Z4	epitope	Overall, our data do not support the inclusion of the 5D5 N-CSP epitope into the next generation of CSP-based vaccines.
36435935	8	0	gly	N-glycosylation	1142:1156	arg2	closely spaced N-glycosylation sites			closely spaced N-glycosylation sites						sites	This alternate priming allows OST to efficiently process closely spaced N-glycosylation sites.
37354227	5	105	part_of	protein	1041:1047	arg1	glycosylation sites	protein		glycosylation sites		Fterm	Site	protein		sites	Our data reveal that the amino acid substitutions and deletions between variants impact the abundance and type of glycans on glycosylation sites of the spike protein.
35960654	5	17	gly	N-glycopeptides	981:995	arg2	3514 quantifiable N-glycopeptides			3514 quantifiable N-glycopeptides						N-glycopeptides	With the integration of a one-tube sample processing workflow and high-pH fractionation, 3514 quantifiable N-glycopeptides were identified from 30 μg HeLa cell tryptic digests with reliable quantification performance.
34695439	7	40	part_of	TSR	1135:1137	arg1	TSR domains 1 and 3	TSR		TSR domains 1 and 3		Cterm	Site	TSR		domains	C-mannosylation was variably present on WT TSP1 and increased on TSR domains 1 and 3 in KO cells.
34726173	7	8	gly	glycosylation	1743:1755	arg1	the SDR domain			the SDR domain						domain	Overall, these findings not only elucidate the putative role of the three domains of SdgB in recognizing donor and acceptor substrates, but also provide new mechanistic insights into glycosylation of the SDR domain, which can serve as a starting point for the development of antibacterial drugs against staphylococcal infections.
36385894	7	95	gly	glycopeptides	1154:1166	arg2	The glycopeptides			The glycopeptides						glycopeptides	The glycopeptides involved proteins in complement and coagulation cascades (p adj = 6.418 × 10-4), innate immunity (p adj = 6.094 × 10-3), acute inflammatory response (p adj = 6.404 × 10-5), defense response (p adj = 2.082 × 10-4), complement activation pathways (p adj = 1.895 × 10-2), and immunoglobulin-mediated immune response pathways (p adj = 4.818 × 10-2).
36018613	9	92	gly	peptide	873:879	arg1	charged glycan and peptide fragments				charged glycan and peptide fragments						We then interrogate the structure(s) of the resulting charged glycan and peptide fragments with infrared "action" spectroscopy.
35995381	4	32	gly	fucosylated	767:777	arg1	all fucosylated N-glycans				all fucosylated N-glycans						The sulfated complex and hybrid type N-glycan species were determined by the analysis of the human Tg samples with HPLC-HILIC-FLD-MS/MS. It was found that all fucosylated N-glycans carried fucose residue on their N-glycan core structure.
32709309	1	1	gly	glycoproteins	204:216	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity.
32709309	1	23	gly	released	190:197	arg1	glycoproteins AND glycans	glycoproteins			glycans	Fterm		glycoproteins			Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity.
32898162	0	42	part_of	linchpin	41:48	arg1	a homology-independent linchpin domain	linchpin		a homology-independent linchpin domain		Fterm	Site	linchpin		domain	Identification of a homology-independent linchpin domain controlling mouse and bank vole prion protein conversion.
32691273	13	21	gly	proteins	1890:1897	arg1	reduced O-GlcNAcylation	proteins			reduced O-GlcNAcylation	Fterm		proteins			Moreover, reduced O-GlcNAcylation of the total proteins by the shRNA-mediated silencing of the OGT gene, which is the only gene to modify proteins with the O-GlcNAc molecule, reversed the insulin resistance and endoplasmic reticulum stress phenotype, even with the externally stimulated O-GlcNAc modification conditions.
32691273	13	109	gly	proteins	1981:1988	arg1	the O-GlcNAc molecule	proteins			the O-GlcNAc molecule	Fterm		proteins			Moreover, reduced O-GlcNAcylation of the total proteins by the shRNA-mediated silencing of the OGT gene, which is the only gene to modify proteins with the O-GlcNAc molecule, reversed the insulin resistance and endoplasmic reticulum stress phenotype, even with the externally stimulated O-GlcNAc modification conditions.
34960798	8	24	part_of	sequences	1377:1385	arg1	CA protein	protein		sequences		Fterm	Site	protein		sequences	Amino acid (aa) sequences of immunodominant epitopes in CA protein were well conserved while Major Homology Region (MHR) had more alteration showing unique mutations in sequences of subtypes A5 and A17.
34960798	8	31	part_of	protein	1420:1426	arg1	Amino acid (aa) sequences	protein		Amino acid (aa) sequences		Fterm	Site	protein		sequences	Amino acid (aa) sequences of immunodominant epitopes in CA protein were well conserved while Major Homology Region (MHR) had more alteration showing unique mutations in sequences of subtypes A5 and A17.
34960798	8	92	part_of	epitopes	1405:1412	arg1	CA protein	protein		epitopes		Fterm	Site	protein		epitopes	Amino acid (aa) sequences of immunodominant epitopes in CA protein were well conserved while Major Homology Region (MHR) had more alteration showing unique mutations in sequences of subtypes A5 and A17.
36252443	1	26	gly	glycoproteins	114:126	arg1	Milk glycoproteins	Milk glycoproteins				Fterm		glycoproteins			Milk glycoproteins play various biological roles including antibacterial, antiviral activities, modulating immune responses in living organisms.
36999031	6	5	gly	fucosylated	1463:1473	arg1	all fucosylated digalactosylated structures				all fucosylated digalactosylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36999031	6	11	gly	disialylated	1219:1230	arg1	all fucosylated monosyalilated and disialylated structures				all fucosylated monosyalilated and disialylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36999031	6	98	gly	fucosylated	1188:1198	arg1	all fucosylated monosyalilated and disialylated structures				all fucosylated monosyalilated and disialylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36999031	6	101	gly	fucosylated	1361:1371	arg1	all fucosylated structures				all fucosylated structures						Results In the discovery population, the adjusted OR of GP20 (digalactosylated monosialylated biantennary with core and antennary fucose), IGP33 (the ratio of all fucosylated monosyalilated and disialylated structures), IGP44 (the proportion of high mannose glycan structures in total neutral IgG glycans), IGP58 (the percentage of all fucosylated structures in total neutral IgG glycans), IGP75 (the incidence of bisecting GlcNAc in all fucosylated digalactosylated structures in total neutral IgG glycans), and the glycan score are 4.03 (95% CI: 3.03-5.36, P<0.001), 0.69 (95% CI: 0.55-0.87, P<0.001), 0.56 (95% CI: 0.45-0.69, P<0.001), 0.52 (95% CI: 0.41-0.65, P<0.001), 7.17 (95% CI: 4.77-10.79, P<0.001), and 2.86 (95% CI: 2.33-3.53, P<0.001), respectively.
36740532	1	65	gly	glycoprotein	98:109	arg1	BACKGROUND Platelet glycoprotein	BACKGROUND Platelet glycoprotein				Fterm		glycoprotein			BACKGROUND Platelet glycoprotein (GP) Ibα is the major ligand-binding subunit of the GPIb-IX-V complex that binds von Willebrand factor.
35304921	8	40	part_of	EGF-like	996:1003	arg1	an EGF-like domain	EGF		an EGF-like domain		OGER	Site	EGF	P01133	domain	An O-linked HexNAc was found in an EGF-like domain of β1 integrin.
35304921	8	48	part_of	integrin	1018:1025	arg1	an EGF-like domain	integrin		an EGF-like domain		Fterm	Site	integrin		domain	An O-linked HexNAc was found in an EGF-like domain of β1 integrin.
35877740	6	38	part_of	1→	865:866	arg1	→4)-α-l-Rhap-(1→ residues	Rhap-(1		→4)-α-l-Rhap-(1→ residues		OGER	Site	Rhap-(1		residues	Partial glycosylation was at C-2 of →4)-α-l-Rhap-(1→ residues.
35877740	6	46	part_of	-α-l-Rhap-	854:863	arg1	→4)-α-l-Rhap-(1→ residues	Rhap-(1		→4)-α-l-Rhap-(1→ residues		OGER	Site	Rhap-(1		residues	Partial glycosylation was at C-2 of →4)-α-l-Rhap-(1→ residues.
36089065	1	17	gly	glycoprotein	208:219	arg1	various glycoprotein properties	various glycoprotein properties				Fterm		glycoprotein			N-glycosylation is an essential eukaryotic posttranslational modification that affects various glycoprotein properties, including folding, solubility, protein-protein interactions, and half-life.
36192063	6	98	part_of	N-glycoproteins	1380:1394	arg1	521 differentially expressed intact N-glycopeptides	N-glycoproteins		521 differentially expressed intact N-glycopeptides		Fterm	Site	N-glycoproteins		N-glycopeptides	In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36192063	5	37	gly	N-glycopeptides	873:887	arg2	TMT-labeled intact N-glycopeptides			TMT-labeled intact N-glycopeptides						N-glycopeptides	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
36192063	5	44	gly	N-glycosite	1216:1226	arg2	N-glycosite			N-glycosite						N-glycosite	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
36192063	5	84	gly	N-glycopeptide	1176:1189	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
36252443	8	27	gly	glycoproteins	1374:1386	arg1	dairy glycoproteins	dairy glycoproteins				Fterm		glycoproteins			In this study, the activity of EndoBI-1 was compared to a commercial enzyme to release N-glycans, the peptide-N-glycosidase F (PNGase F), using dairy glycoproteins as the substrate.
37156312	0	65	gly	glycosites	49:58	arg2	human O-linked threonine glycosites			human O-linked threonine glycosites						glycosites	O-GlyThr: Prediction of human O-linked threonine glycosites using multi-feature fusion.
37266972	0	54	gly	glycoproteins	39:51	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.
37266972	0	95	gly	deglycosylation	8:22	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			In vivo deglycosylation of recombinant glycoproteins in tobacco BY-2 cells.
36478308	2	87	part_of	peptides	376:383	arg1	single, isolated glycosylation sites	peptides		single, isolated glycosylation sites						sites	The method has been optimised to produce peptides with single, isolated glycosylation sites using multiple protease digests.
35262690	10	75	gly	hyposialylation	1504:1518	arg1	O-glycans				O-glycans						It also points towards a possible mechanism behind the hyposialylation of N and O-glycans.
33904933	7	85	part_of	epitope	1480:1486	arg1	annexin A2 protein	annexin A2 protein		epitope		PUBTATOR	Site	annexin A2 protein	302	epitope	Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, si-RNA silencing analysis, enzymatic deglycosylation, and tunicamycin treatment revealed that 12G5A recognized the glycosylated epitope on annexin A2 protein.
36660462	7	80	gly	glycoproteins	1243:1255	arg1	10 glycoproteins	10 glycoproteins				Fterm		glycoproteins			Through a combined analysis of proteomic and glycoproteomic data, it was found that the changes in 10 glycoproteins were caused by the difference in glycosylation level but not in the protein abundance level.
37037133	1	35	gly	glycosylated	210:221	arg1	Milk fat globule membrane (MFGM) proteins	Milk fat globule membrane (MFGM) proteins				Fterm		proteins			Milk fat globule membrane (MFGM) proteins are highly glycosylated and involved in various biological processes within the body.
36289103	8	24	gly	sialylated	1099:1108	arg1	α2-6 linked sialylated N-glycans				α2-6 linked sialylated N-glycans						Importantly, α2-6 linked sialylated N-glycans were dominant across most HNSCC cell lines except in SCC-9 cells where similar levels of α2-6 and α2-3 sialylated N-glycans were observed.
36289103	8	59	gly	sialylated	1223:1232	arg1	α2-6 and α2-3 sialylated N-glycans				α2-6 and α2-3 sialylated N-glycans						Importantly, α2-6 linked sialylated N-glycans were dominant across most HNSCC cell lines except in SCC-9 cells where similar levels of α2-6 and α2-3 sialylated N-glycans were observed.
37366623	2	112	gly	glycoprotein	395:406	arg1	envelope glycoprotein B	envelope glycoprotein B				Fterm		glycoprotein B			Here we show in cell cultures that an N-glycan shield on the herpes simplex virus 1 (HSV-1) envelope glycoprotein B (gB) mediated evasion from neutralization and antibody-dependent cellular cytotoxicity due to pooled γ-globulins derived from human blood.
35889277	2	14	gly	glycoproteins	377:389	arg1	glycoproteins	glycoproteins				Fterm		structure of glycoproteins			Hence, the development of analytical methods able to characterize the composition and structure of glycoproteins is crucial.
32357974	6	95	part_of	N-glycosylated	1191:1204	arg1	nascently N-glycosylated polypeptides	N-glycosylated		nascently N-glycosylated polypeptides		Cterm	Site	N-glycosylated		polypeptides	This finding, demonstrated previously for a handful of deamidated T cell epitopes, implicates a more global role for the retrograde transport of nascently N-glycosylated polypeptides from the ER and their subsequent degradation within the cytosol to form HLA-ligand precursors.
37211816	5	54	gly	N-glycosylation	1224:1238	arg2	optimal artificial VHH N-glycosylation sites			optimal artificial VHH N-glycosylation sites						sites	The set of optimal artificial VHH N-glycosylation sites identified in this study can serve as a blueprint for targeted glyco-engineering of other VHHs, enabling site-specific functionalization through the rapidly expanding toolbox of synthetic glycobiology.
35766466	8	26	gly	nonsialylated	1273:1285	arg1	nonsialylated N-glycans				nonsialylated N-glycans						Improving detection of nonsialylated N-glycans allows for a more thorough analysis of specific structural features such as fucosylation or branching, particularly of low abundant structures.
36189205	6	47	gly	found	1220:1224	arg1	Asn162 AND a glycan structure	FcγRIIIa		Asn162	a glycan structure	PUBTATOR		FcγRIIIa	2214	Asn162	Our study confirms that afucosylated IgG1 has the highest binding affinity to oligomannose FcγRIIIa, a glycan structure commonly found on Asn162 on FcγRIIIa expressed by NK cells but not monocytes or recombinantly expressed FcγRIIIa.
35413075	10	36	gly	α1,3-fucosylation	1468:1484	arg1	glycans				glycans						Among them, glycans with α1,3-fucosylation ((+/- sialyl) Lewis X), triply α2,6-sialylated tri-antennary structures and/or a (Man3)GlcNAc1-core displayed elevated levels in cancer patients.
35413075	10	86	gly	α2,6-sialylated	1517:1531	arg1	triply α2,6-sialylated tri-antennary structures				triply α2,6-sialylated tri-antennary structures						Among them, glycans with α1,3-fucosylation ((+/- sialyl) Lewis X), triply α2,6-sialylated tri-antennary structures and/or a (Man3)GlcNAc1-core displayed elevated levels in cancer patients.
35104505	9	66	part_of	GnT-V	1651:1655	arg1	the N domain	GnT-V		the N domain		PUBTATOR	Site	GnT-V	Q09328	domain	Our findings suggest that the N domain of GnT-V plays a critical role in the recognition of glycoprotein substrates, providing new insights into the mechanism of substrate-selective biosynthesis of N-glycans.
36585837	5	28	gly	glycosylation	785:797	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Glycopeptides were isolated with lectin-based extraction and treated with Peptide N-glycosidase F (PNGase F) to identify N-linked glycosylation sites.
35380435	9	26	gly	glycoproteins	1836:1848	arg1	heterogeneous glycoproteins	heterogeneous glycoproteins				Fterm		glycoproteins			The HILIC top-down MS platform holds great potential in resolving heterogeneous glycoproteins for facile comparison of biosimilars in quality control applications.
37266972	10	21	gly	18 N-glycosylation	1290:1307	arg2	18 N-glycosylation sites			18 N-glycosylation sites						sites	Likewise, extensive deglycosylation of glycoprotein B, which possesses 18 N-glycosylation sites, was observed.
37289618	6	15	gly	sialylated	1121:1130	arg1	sialylated N-linked glycans				sialylated N-linked glycans						This new rule grants confident glycan annotations and compositions beyond accurate mass measurements, thereby further improving the capability of IR-MALDESI to study sialylated N-linked glycans within biological tissues.
35775947	5	45	gly	glycosylated	1192:1203	arg1	the galactose residue				the galactose residue						Starting with a common lactose precursor, the sulfur function was incorporated by double inversion of the configuration of the galactose residue that was further glycosylated using different activated donors.
32719124	3	15	gly	glycosylation	386:398	arg2	an N-linked glycosylation site			an N-linked glycosylation site						site	By engineering a glycoprotein-derived epitope to contain an N-linked glycosylation site, we determined that optimal CD8+ T cell expansion and function were induced by the peptides that are rapidly produced from the exceedingly minor fraction of protein mislocalized to the cytosol.
36830744	7	20	gly	composition	1251:1261	arg1	CAD			position	CAD					position	The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD.
37341462	5	14	gly	O-glycopeptide	946:959	arg2	O-glycopeptide spectra			O-glycopeptide spectra						O-glycopeptide	In this strategy, theoretical O-glycan Y-ion patterns are constructed to match the experimental Y-ions in O-glycopeptide spectra, which enables the determination of the mass of some glycans and results in the reduction of searching space.
36935145	3	23	gly	glycoproteins	1024:1036	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Both LoG and GoG generates new structures and functions of glycoproteins, which has been observed in the S protein of SARS-Cov-2 as well as malignant diseases.
37348475	5	16	gly	proteins	1079:1086	arg1	the glycan structures	proteins			the glycan structures	Fterm		proteins			Comparison of the glycan structures of the microsomal SPO-AGP and the secreted SPO-AGPΔC using antibodies against the glycan epitopes of AGP indicated that the glycan structures of these proteins are different.
35104505	7	6	gly	glycoprotein	1329:1340	arg1	the glycoprotein substrates	the glycoprotein substrates				Fterm		glycoprotein			Surprisingly, GnT-VΔN showed substantially reduced activity toward the glycoprotein substrates, whereas it almost fully maintained its activity toward the oligosaccharides and the glycopeptide substrates.
35104505	7	21	gly	glycopeptide	1438:1449	arg2	the glycopeptide substrates			the glycopeptide substrates						glycopeptide	Surprisingly, GnT-VΔN showed substantially reduced activity toward the glycoprotein substrates, whereas it almost fully maintained its activity toward the oligosaccharides and the glycopeptide substrates.
32273875	2	46	gly	N239	268:271	arg1	Oligomannose carbohydrates			N239	Oligomannose carbohydrates					N239	Oligomannose carbohydrates at N239 and/or N2118 on factor VIII allow its binding to the macrophage mannose receptor expressed on human dendritic cells, thereby leading to factor VIII endocytosis and presentation to CD4+ T lymphocytes.
32273875	2	51	gly	N2118	280:284	arg1	Oligomannose carbohydrates			N2118	Oligomannose carbohydrates					N2118	Oligomannose carbohydrates at N239 and/or N2118 on factor VIII allow its binding to the macrophage mannose receptor expressed on human dendritic cells, thereby leading to factor VIII endocytosis and presentation to CD4+ T lymphocytes.
35887202	2	8	gly	glycosylation	280:292	arg1	MUC1				MUC1						This is based on the fact that MUC1 undergoes aberrant glycosylation upon cancer progression, and anti-MUC1 antibodies differentiate changes in glycan structure.
36830744	7	58	part_of	IgG	1237:1239	arg1	the IgG N-glycome composition	IgG		the IgG N-glycome composition		Cterm	Site	IgG		position	The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD.
35388686	1	35	gly	proteins	250:257	arg1	monosaccharide linkage patterns	proteins			monosaccharide linkage patterns	Fterm		proteins			Characterization of protein glycosylation by tandem mass spectrometry remains challenging owing to the vast diversity of oligosaccharides bound to proteins, the variation in monosaccharide linkage patterns, and the lability of the linkage between the glycan and protein.
35285541	2	9	gly	glycoproteins	274:286	arg1	wherein	wherein				Fterm		wherein			Majority of the biopharmaceuticals are glycoproteins, wherein about 1 to 30% of their molecular weight (MW) are contributed by the glycans.
35285541	2	9	gly	glycoproteins	274:286	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Majority of the biopharmaceuticals are glycoproteins, wherein about 1 to 30% of their molecular weight (MW) are contributed by the glycans.
35766466	0	33	gly	Nonsialylated	45:57	arg1	Nonsialylated N-Linked Glycans				Nonsialylated N-Linked Glycans						Novel Combined Enzymatic Approach to Analyze Nonsialylated N-Linked Glycans through MALDI Imaging Mass Spectrometry.
34452239	4	85	gly	glycopeptide	981:992	arg2	the glycopeptide			the glycopeptide						glycopeptide	We have synthesized the native Ang (1-7) peptide and the glycopeptide, PNA5, and have formulated them for targeted respiratory delivery as inhalable dry powders.
36534501	7	64	part_of	MMP-14	1274:1279	arg1	the catalytic domain	MMP-14		the catalytic domain		PUBTATOR	Site	MMP-14	4323	domain	Molecular docking analysis showed that all SLRPs bind to MMP-14 through their concave face, but in different regions of the catalytic domain of MMP-14.
35022995	7	25	part_of	NXT/S	1055:1059	arg1	the NXT/S sequon	NXT		the NXT/S sequon		PUBTATOR	Site	NXT	29107	sequon	Asn143 displayed complex-type glycosylation, suggesting that oligosaccharide transferase may recognize the NXT/S sequon in the secretory form, but not clearly in full-length dynAP.
32592613	3	43	part_of	IGFBP-3	544:550	arg1	the 18-amino acid IGFBP-3 peptide	IGFBP-3		the 18-amino acid IGFBP-3 peptide		OGER	Site	IGFBP-3	P17936	peptide	We previously showed that the 18-amino acid IGFBP-3 peptide is capable of binding either HA or HN with comparable affinities to the full-length IGFBP-3 protein and that IGFBP-3 can compete with the HA receptor, CD44, for binding HA.
37354227	0	75	gly	N-glycosylation	16:30	arg1	the spike proteins	the spike proteins				Fterm		proteins			Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2.
36512245	1	4	gly	glycosylation	158:170	arg2	the major sites			the major sites						sites	The Golgi apparatus is one of the major sites of protein and lipid glycosylation and processing.
34379775	7	75	gly	fucosylated	1380:1390	arg1	fucosylated glycan				fucosylated glycan						There was a higher abundance of the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc), fucosylated glycan (GlcNAcβ1-3(Fucα1-2Galβ1-3)GalNAc) and core 4 glycan (GlcNAcβ1-3[GlcNAcβ1-6]GalNAc) in the low-fertility Suffolk breed compared with NWS (high fertility).
34379775	7	75	gly	fucosylated	1380:1390	arg1	the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc)				the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc)						There was a higher abundance of the sulfated glycan (Galβ1-3[SO3-GlcNAcβ1-6]GalNAc), fucosylated glycan (GlcNAcβ1-3(Fucα1-2Galβ1-3)GalNAc) and core 4 glycan (GlcNAcβ1-3[GlcNAcβ1-6]GalNAc) in the low-fertility Suffolk breed compared with NWS (high fertility).
36797772	1	26	gly	glycoproteins	101:113	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The role of glycoproteins as key cell surface molecules during development and stress is well established, yet the relationship between their structural features and functional mechanisms are poorly defined.
35373202	6	16	gly	glycosylation	1158:1170	arg1	acceptor sites			acceptor sites						sites	Our experiments suggest that during this process, neighboring tandem repeats are critical for further glycosylation of acceptor sites by GalNAc-T2/T4 in a lectin-assisted manner.
34633871	9	28	gly	glycoproteins	1296:1308	arg1	volcanii glycoproteins	volcanii glycoproteins				Fterm		glycoproteins			volcanii glycoproteins.
36350770	5	9	part_of	AAT	1013:1015	arg1	the neighboring AAT residues	AAT		the neighboring AAT residues		OGER	Site	AAT	P01009	residues	Structural investigation showed the oligomers to adopt a right-handed helical structure with the acetyl esters exposed on the periphery of the helix in close proximity of the neighboring AAT residues, thereby imposing conformational restrictions on the AATα1-4GalA(3OAc) glycosidic linkages, supporting the helical shape of the polysaccharide, that has been proposed to be critical for its unique biological activity.
34831340	4	4	gly	sialylated	631:640	arg1	sialylated N-linked glycans				sialylated N-linked glycans						We conducted a HPLC analysis of sialylated N-linked glycans released from total plasma proteins and characterized the α1,2-mannosidase I activity of the lymphocyte microsome fraction.
34831340	4	12	gly	released	659:666	arg1	total plasma proteins AND sialylated N-linked glycans	total plasma proteins			sialylated N-linked glycans	Fterm		proteins			We conducted a HPLC analysis of sialylated N-linked glycans released from total plasma proteins and characterized the α1,2-mannosidase I activity of the lymphocyte microsome fraction.
34611869	3	79	gly	glycans	653:659	arg1	the CH2 domains			the CH2 domains	the CH2 domains		Site			domains	In this report we analyzed four antibodies available in the PDB (1IGT, 1IGY, 1HZH, and 5DK3) using the online software UCSF Chimera to study the structural features of the proteins and the associated N-linked glycans in the CH2 domains of the Fc region.
34611869	3	79	gly	glycans	653:659	arg1	the Fc region			the Fc region	the Fc region		Site			region	In this report we analyzed four antibodies available in the PDB (1IGT, 1IGY, 1HZH, and 5DK3) using the online software UCSF Chimera to study the structural features of the proteins and the associated N-linked glycans in the CH2 domains of the Fc region.
37202422	5	12	part_of	IgG1	708:711	arg1	the Fc fragment	IgG1		the Fc fragment		OGER	Site	IgG1	P01857	fragment	Here, we present the structure of one such nanobody, X0, in complex with the Fc fragment of afucosylated IgG1.
37202422	5	46	part_of	Fc	680:681	arg1	the Fc fragment	Fc		the Fc fragment		Cterm	Site	Fc		fragment	Here, we present the structure of one such nanobody, X0, in complex with the Fc fragment of afucosylated IgG1.
33082260	10	17	part_of	structure	2330:2338	arg1	the apicomplexan parasite	structure of the GNA1		the apicomplexan parasite		OGER	Site	structure of the GNA1	Q96EK6	parasite	The high-resolution crystal structure of the GNA1 ortholog from the apicomplexan parasite C. parvum, used here as a surrogate, highlights significant differences from human GNA1.
33082260	10	31	part_of	parasite	2383:2390	arg1	The high-resolution crystal structure	structure of the GNA1		parasite		OGER	Site	structure of the GNA1	Q96EK6	parasite	The high-resolution crystal structure of the GNA1 ortholog from the apicomplexan parasite C. parvum, used here as a surrogate, highlights significant differences from human GNA1.
36181511	5	49	gly	glycopeptide	1044:1055	arg2	an optional glycopeptide enrichment approach			an optional glycopeptide enrichment approach						glycopeptide	In conclusion, cotton HILIC can be used as an optional glycopeptide enrichment approach in glycosylation analysis with its specific merit.
36377874	1	100	gly	glycosylation	175:187	arg2	the glycosylation site			the glycosylation site						site	The glycan loop of Zika virus (ZIKV) envelope protein (E) contains the glycosylation site and has been well documented to be important for viral pathogenesis and transmission.
35038468	1	22	gly	used	312:315	arg2	GLU-CPS-1			GLU-CPS-1						GLU	Two capsular polysaccharides (WL-CPS-1 and GLU-CPS-1) purified from Nostoc flagelliforme under normal and mixotrophic culture conditions were used to investigate the hypolipidemic activity and effect on intestinal flora in C57BL/6J mice respectively.
36007953	5	8	gly	core-fucosylated	957:972	arg1	the core-fucosylated N-glycans				the core-fucosylated N-glycans						The affinities were at a low nanomolar level, which were ~ 1000-fold stronger than those between PhoSL and the core-fucosylated N-glycans at the micromolar level.
36977665	2	7	gly	sequence	318:325	arg1	sequence glycan				sequence glycan						Nanopore-based single-molecule sensing offers the potential to elucidate glycan structure and even sequence glycan.
32417172	9	56	gly	glycosylated	1318:1329	arg1	the glycosylated sites			the glycosylated sites						sites	Ser121 residue was one of the glycosylated sites on p53.
37173020	2	46	gly	heterogeneity	491:503	arg1	pectin	pectin				Fterm		pectin			Numerous bioactive polysaccharides associated with pectin are newly reported every year, but the general mechanism of their immunological action remains unclear owing to the complexity and heterogeneity of pectin.
33244686	6	29	gly	N-glycosylation	1695:1709	arg2	five N-glycosylation sites			five N-glycosylation sites						sites	We demonstrated that with enhanced detection sensitivity, IP-HILIC-MS can confidently identify an increased number of site-specific N-linked glycans for IgG1, and IgG4 mAbs as well as an Fc-domain fusion protein (containing five N-glycosylation sites) through MS/MS-based search in the data-dependent acquisition mode, meanwhile, achieve comparable quantitative results compared with the traditional methods.
36007953	4	44	gly	core-fucosylated	818:833	arg1	core-fucosylated N-glycans				core-fucosylated N-glycans						Here, we observed that the S proteins of the ancestral (Wuhan) and Omicron strains bind with Pholiota squarrosa lectin (PhoSL), a 40-amino-acid chemically synthesised peptide specific to core-fucosylated N-glycans.
35616904	8	12	gly	glycoproteins	1380:1392	arg1	their shared glycoproteins	their shared glycoproteins				Fterm		glycoproteins			Comparison of site-specific glycans between seminal plasma and spermatozoa revealed more complicated glycan structures in seminal plasma than in spermatozoa, even on their shared glycoproteins.
35977913	2	8	gly	N-glycosylated	143:156	arg1	N-glycosylated proteins	N-glycosylated proteins				Fterm		proteins			However, N-glycosylated proteins from natural sources exhibit considerable heterogeneity in the appended oligosaccharides, bringing daunting challenges to corresponding basic research and therapeutic applications.
35960654	1	18	gly	glycopeptide	134:145	arg2	Intact glycopeptide analysis			Intact glycopeptide analysis						glycopeptide	Intact glycopeptide analysis has been of great interest because it can elucidate glycosylation site information and glycan structural composition at the same time.
35960654	1	61	gly	glycosylation	208:220	arg2	glycosylation site information			glycosylation site information						site	Intact glycopeptide analysis has been of great interest because it can elucidate glycosylation site information and glycan structural composition at the same time.
36095241	1	38	part_of	protein	213:219	arg1	the receptor binding domain	S) protein		the receptor binding domain		OGER	Site	S) protein	Q15517	domain	Harnessing highly conserved peptides derived from the receptor binding domain (RBD) of spike (S) protein to construct peptide-based inhibitors is one of the most effective strategies to fight against the ever-mutating coronavirus SARS-CoV-2.
37316505	0	17	part_of	protein	90:96	arg1	the stalk region	PD-1 protein		the stalk region		OGER	Site	PD-1 protein	Q15116	region	Intact mass analysis reveals the novel O-linked glycosylation on the stalk region of PD-1 protein.
36732731	7	122	gly	fucosylated	1290:1300	arg1	multiple fucosylated glycans				multiple fucosylated glycans						Lectin like activity at the N-terminal of TpsA due to a conserved hemagglutinin domain (Pfam identifier [ID] PF05860) mediates binding to mucins that carry multiple fucosylated glycans.
33446867	1	5	gly	glycosylation	190:202	arg1	proteins	proteins				Fterm		proteins			The oligosaccharide required for asparagine (N)-linked glycosylation of proteins in the endoplasmic reticulum (ER) is donated by the glycolipid Glc3Man9GlcNAc2-PP-dolichol.
36182101	8	59	gly	fucosylated	1552:1562	arg1	fucosylated structures				fucosylated structures						Statistical correlation analysis, binding experiments with J99 wt, and J99ΔbabAΔsabA and inhibition experiments using synthetic glycoconjugates demonstrated that the differences in H. pylori-binding ability among these four groups were governed by BabA-dependent binding to fucosylated structures.
31996426	6	67	gly	glycoprotein	922:933	arg1	the viral glycoprotein M envelope protein	the viral glycoprotein M envelope protein				Fterm		glycoprotein M			Despite the marked antimicrobial action of eBD2 and -3 against many bacterial and viral pathogens, EHV1 virions were resistant to eBDs through the action of the viral glycoprotein M envelope protein.
35854001	8	41	part_of	lectin	1107:1112	arg1	the lectin domain	lectin		the lectin domain		Fterm	Site	lectin	269181	domain	Point mutation of the residue critical to sugar recognition impairs the enzymatic activity, suggesting that the lectin domain is a regulatory subunit for efficient catalytic reaction.
35921896	12	64	gly	heterogeneity	1568:1580	arg1	CD16a F158 backbone amide and N162 glycan resonances				CD16a F158 backbone amide and N162 glycan resonances						We also identified conformational heterogeneity of CD16a F158 backbone amide and N162 glycan resonances using NMR spectroscopy.
36925257	3	17	part_of	L-ESP	575:579	arg1	the composition	ESP		the composition		OGER	Site	ESP	Q6UW49	position	Here, we first prepared fermented Eucheuma spinosum polysaccharides (F-ESP) by Lactobacillus fermentation, compared with low-temperature freeze-thaw ESP (L-ESP) prepared by the freeze-thaw method, explored the composition and structural characteristics of F-ESP and L-ESP, and evaluation of the ability of different samples to inhibit mast cell degranulation using classical mast cell model.
36925257	3	25	part_of	F-ESP	565:569	arg1	the composition	ESP		the composition		OGER	Site	ESP	Q6UW49	position	Here, we first prepared fermented Eucheuma spinosum polysaccharides (F-ESP) by Lactobacillus fermentation, compared with low-temperature freeze-thaw ESP (L-ESP) prepared by the freeze-thaw method, explored the composition and structural characteristics of F-ESP and L-ESP, and evaluation of the ability of different samples to inhibit mast cell degranulation using classical mast cell model.
35179194	8	103	gly	-fucosylated	1328:1339	arg1	α(1,2)-fucosylated structures				α(1,2)-fucosylated structures						Genomic analyses of the mothers were not performed; instead, milk was phenotyped according to the abundances of α(1,2)-fucosylated structures.
32839225	11	75	part_of	ACO2	1870:1873	arg1	the catalytic site	ACO2		the catalytic site		PUBTATOR	Site	ACO2	11429	site	Computer modeling show that N612 sits atop the catalytic site of ACO2.
36268609	5	0	part_of	laminin-α2	871:880	arg1	laminin-G-like domains 4 and 5	laminin-α2		laminin-G-like domains 4 and 5		PUBTATOR	Site	laminin-α2	3908	domains	We also investigated interactions between matriglycan hexasaccharides and laminin-G-like domains 4 and 5 of laminin-α2 using saturation transfer difference-NMR.
36268609	5	3	part_of	laminin-G-like	837:850	arg1	laminin-G-like domains 4 and 5	laminin		laminin-G-like domains 4 and 5		OGER	Site	laminin		domains	We also investigated interactions between matriglycan hexasaccharides and laminin-G-like domains 4 and 5 of laminin-α2 using saturation transfer difference-NMR.
36698045	4	30	gly	N-glycosylated	559:572	arg1	a surface protein	a surface protein				Fterm		protein			As a surface protein on the virus envelop, the spike was reported to be heavily N-glycosylated and glycosylation had a great impact on its immunogenicity and efficacy.
36740532	10	76	gly	macroglycopeptide	1366:1382	arg2	macroglycopeptide domain			macroglycopeptide domain						macroglycopeptide domain	Glycosites are also located within the macroglycopeptide domain and mechanosensory domain.
35889277	5	7	gly	glycoproteins	839:851	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
35889277	5	23	gly	composition	957:967	arg1	monosaccharides			position	monosaccharides					position	A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
35889277	5	23	gly	composition	957:967	arg1	glycans			position	glycans					position	A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
35889277	5	28	gly	glycoproteins	872:884	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			A database of hyperspectral Raman imaging data of glycoproteins was built, and the glycoproteins were characterized by LC-FLR-MS as a reference method to determine the composition in glycans and monosaccharides.
37266972	1	84	gly	glycoproteins	117:129	arg1	recombinant pharmaceutical glycoproteins	recombinant pharmaceutical glycoproteins				Fterm		glycoproteins			Production of recombinant pharmaceutical glycoproteins has been carried out in multiple expression systems.
36447399	5	30	gly	N-glycosylation	1193:1207	arg2	N-glycosylation sites			N-glycosylation sites						sites	Since only about 20% of human IgG1 have a Fab glycan, we extended the application of this approach by using molecular modeling to introduce N-glycosylation sites in the Fab constant region of other therapeutic monoclonal antibodies.
36373229	8	9	gly	O-glycosylated	1826:1839	arg1	densely O-glycosylated mucin-domain glycoproteins	densely O-glycosylated mucin-domain glycoproteins				Fterm		glycoproteins			We use this platform to generate a consensus motif for the recently characterized immunomodulating metalloprotease (IMPa) from Pseudomonas aeruginosa and show that IMPa is a favorable O-glycoprotease for characterizing densely O-glycosylated mucin-domain glycoproteins.
36373229	8	11	gly	glycoproteins	1854:1866	arg1	densely O-glycosylated mucin-domain glycoproteins	densely O-glycosylated mucin-domain glycoproteins				Fterm		glycoproteins			We use this platform to generate a consensus motif for the recently characterized immunomodulating metalloprotease (IMPa) from Pseudomonas aeruginosa and show that IMPa is a favorable O-glycoprotease for characterizing densely O-glycosylated mucin-domain glycoproteins.
35647713	3	65	gly	glycoprotein	560:571	arg1	viral glycoprotein immunogens	viral glycoprotein immunogens				Fterm		glycoprotein			There is, however, a lack of methods to conjugate small molecule immune potentiators to viral glycoprotein immunogens without compromising epitope integrity.
33199824	1	38	part_of	proteins	248:255	arg1	threonine	proteins		threonine		Fterm	AminoAcid	proteins		threonine residues	O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification which occurs on the hydroxyl group of serine or threonine residues of nucleocytoplasmic proteins.
33199824	1	38	part_of	proteins	248:255	arg1	serine	proteins		serine		Fterm	AminoAcid	proteins		serine	O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification which occurs on the hydroxyl group of serine or threonine residues of nucleocytoplasmic proteins.
37060669	2	38	gly	fucosylated	240:250	arg1	a fucosylated trisaccharide				a fucosylated trisaccharide						In this study, a fucosylated trisaccharide (GFG) was separated from the acidolysis products of exopolysaccharides from Clavibacter michiganensis M1.
37184080	3	14	gly	position	967:974	arg1	4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification			position	4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification					position	Using FLATn-Li, we elucidated the lipid A structure from several bacterial species, including novel structures from arctic bacterioplankton of the Duganella and Massilia genera that favor 4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification at the 1-phosphate position and that demonstrate double glycosylation with Ara4N at the 1 and 4' phosphate positions simultaneously.
37184080	3	60	gly	glycosylation	1004:1016	arg2	the 1 and 4' phosphate positions			positions						positions	Using FLATn-Li, we elucidated the lipid A structure from several bacterial species, including novel structures from arctic bacterioplankton of the Duganella and Massilia genera that favor 4-amino-4-deoxy-l-arabinopyranose (Ara4N) modification at the 1-phosphate position and that demonstrate double glycosylation with Ara4N at the 1 and 4' phosphate positions simultaneously.
33244686	6	65	part_of	containing	1679:1688	arg1	an Fc-domain fusion protein AND five N-glycosylation sites	an Fc-domain fusion protein		five N-glycosylation sites		Fterm	Site	protein		sites	We demonstrated that with enhanced detection sensitivity, IP-HILIC-MS can confidently identify an increased number of site-specific N-linked glycans for IgG1, and IgG4 mAbs as well as an Fc-domain fusion protein (containing five N-glycosylation sites) through MS/MS-based search in the data-dependent acquisition mode, meanwhile, achieve comparable quantitative results compared with the traditional methods.
34939082	3	28	gly	sites	475:479	arg1	glycan modification			sites	glycan modification					sites	Here, we describe a simple, concise graphical representation intended to capture the microheterogeneity associated with glycan modification at specific sites.
35447118	1	32	gly	glycosylation	150:162	arg1	proteins	proteins				Fterm		proteins			Asparagine-linked glycosylation (N-glycosylation) of proteins in the cancer secretome has been gaining increasing attention as a potential biomarker for cancer detection and diagnosis.
35773089	7	76	part_of	IgG	1278:1280	arg1	IgG glycome composition	IgG		IgG glycome composition		PUBTATOR	Site	IgG	668542	position	FINDINGS Multiple statistically significant changes in IgG glycome composition were observed during severe COVID-19.
36985724	2	32	gly	heterogeneity	250:262	arg1	naturally occurring glycoproteins	naturally occurring glycoproteins				Fterm		glycoproteins			Due to N-glycan heterogeneity of naturally occurring glycoproteins, the functions of specific N-glycans on a particular glycoprotein are not always clear.
36985724	2	49	gly	glycoprotein	354:365	arg1	a particular glycoprotein	a particular glycoprotein				Fterm		glycoprotein			Due to N-glycan heterogeneity of naturally occurring glycoproteins, the functions of specific N-glycans on a particular glycoprotein are not always clear.
36985724	2	19	gly	glycoproteins	287:299	arg1	naturally occurring glycoproteins	glycoproteins			N-glycan heterogeneity	Fterm		glycoproteins			Due to N-glycan heterogeneity of naturally occurring glycoproteins, the functions of specific N-glycans on a particular glycoprotein are not always clear.
33082260	4	72	part_of	GNA1	978:981	arg1	an apicomplexan parasite	structure of the GNA1		an apicomplexan parasite		OGER	Site	structure of the GNA1	Q96EK6	parasite	Furthermore, we present the 1.95 Å resolution structure of the GNA1 ortholog from Cryptosporidium parvum, an apicomplexan parasite which is a leading cause of diarrhea in developing countries, as a surrogate for P. falciparum GNA1.
37341462	8	61	gly	O-glycopeptide	1577:1590	arg2	O-glycopeptide			O-glycopeptide						O-glycopeptide	This search mode, the O-Search-Pattern, has been implemented into our database search software, MS-Decipher, and is recommended for searching the O-glycopeptide spectra acquired by sceHCD (stepped collision energy higher-energy collisional dissociation).
35405095	2	34	gly	glycoproteins	383:395	arg1	host glycoproteins	host glycoproteins				Fterm		glycoproteins			The abundance of A. muciniphila is modulated by the presence and accessibility of nutrients, which can be derived from diet or host glycoproteins.
32791164	10	19	gly	glycoprotein	1649:1660	arg1	1				1						Purification and identification revealed deleted in malignant brain tumors 1 (DMBT1) (also known by the aliases GP340 and SALSA), a large glycoprotein with multiple O-glycosylation repeats.
32791164	10	19	gly	glycoprotein	1649:1660	arg1	a large glycoprotein				a large glycoprotein						Purification and identification revealed deleted in malignant brain tumors 1 (DMBT1) (also known by the aliases GP340 and SALSA), a large glycoprotein with multiple O-glycosylation repeats.
36669592	3	44	gly	glycosylation	504:516	arg1	specific proteins	specific proteins				Fterm		proteins			It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation.
35945033	0	41	gly	glycoproteins	193:205	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			PNGase H + variant from Rudaea cellulosilytica with improved deglycosylation efficiency for rapid analysis of eukaryotic N-glycans and hydrogen deuterium exchange mass spectrometry analysis of glycoproteins.
32839225	6	4	gly	sialoglycopeptides	969:986	arg2	N-linked sialoglycopeptides	EL		sialoglycopeptides		Cterm		EL	16891	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg2	N-linked sialoglycopeptides	C4b-binding protein		sialoglycopeptides		PUBTATOR		C4b-binding protein	12269	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg1	testis-expressed protein 101	52, testis-expressed protein 101		sialoglycopeptides		PUBTATOR		52, testis-expressed protein 101	56746	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg1	endothelial lipase	endothelial lipase		sialoglycopeptides		PUBTATOR		endothelial lipase	16891	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32839225	6	4	gly	sialoglycopeptides	969:986	arg1	zonadhesin	zonadhesin		sialoglycopeptides		PUBTATOR		zonadhesin	Q9Y493	sialoglycopeptides	As such, N-linked sialoglycopeptides from C4b-binding protein, endothelial lipase (EL), serine proteases 39 and 52, testis-expressed protein 101 and zonadhesin were reduced following capacitation.
32791352	1	8	gly	glycoprotein	216:227	arg1	homolog glycoprotein complexes	homolog glycoprotein complexes				Fterm		glycoprotein			Species-specific guinea pig cytomegalovirus (GPCMV) causes congenital CMV and the virus encodes homolog glycoprotein complexes to human CMV, including gH-based trimer (gH/gL/gO) and pentamer-complex (PC).
34878920	11	45	gly	Deglycosylation	1553:1567	arg2	N193			N193						N193	Deglycosylation at N193 weakened Env-receptor binding while mutation at N17 influenced Env protein processing.
36648436	6	2	gly	α2,6-sialylated	1187:1201	arg1	oligo-mannose, hybrid-type, and α2,6-sialylated structures				oligo-mannose, hybrid-type, and α2,6-sialylated structures						We furthermore show that ZIP9 knockout affects N-linked glycosylation, resulting in up-regulation of oligo-mannose, hybrid-type, and α2,6-sialylated structures as well as down-regulation of tri- and tetra-antennary structures.
36637420	9	32	gly	N-glycosylation	1746:1760	arg1	Asn-57			Asn-57						Asn-57	These results suggest that N-glycosylation on Asn-57 is required for folding into a functional HAI-2 with full protease suppressive activity and correct subcellular targeting signal.
37398203	9	103	gly	-glycosylation	1532:1545	arg2	This novel N -glycosylation site			This novel N -glycosylation site						site	This novel N -glycosylation site (absent in the germline sequence) is a result of a single mutation giving rise to an NDT motif in the antibody sequence.
35925863	9	61	gly	hypersialylated	1378:1392	arg1	the hyperbranched and hypersialylated glycans				the hyperbranched and hypersialylated glycans						Of significance was the hyperbranched and hypersialylated glycans and their derived glycan subclass traits.
34960798	11	86	part_of	AP-1	2046:2049	arg1	AP-1 sites	AP-1		AP-1 sites		OGER	Site	AP-1		sites	The analysis of LTR sequences revealed that sequences corresponding to the TATA box, AP-4, AML-vis, and polyadenylation signal (poly A) were quite conserved, while considerable alteration was observed in AP-1 sites.
32602701	11	11	part_of	myonectin	1614:1622	arg1	the short collagen domain	myonectin		the short collagen domain		PUBTATOR	Site	myonectin	151176	domain	Most Pro residues within the short collagen domain of myonectin were also hydroxylated, a modification that stabilized the collagen triple helix.
35091091	5	73	gly	glycopeptide	1242:1253	arg2	complex glycopeptide analyses			complex glycopeptide analyses						glycopeptide	Combined with false discovery rate estimation for the glycan assignment, we show that this method is capable of specifically and sensitively identifying glycans in complex glycopeptide analyses and effectively controls the rate of false glycan assignments.
32187935	4	16	gly	glycosylation	560:572	arg2	the potential N-linked glycosylation sites			the potential N-linked glycosylation sites						sites	NetNGlyc 1.0 server was used to predict the potential N-linked glycosylation sites.
35166741	6	44	gly	glycopeptide	1174:1185	arg2	an improved liquid phase glycopeptide coupling			an improved liquid phase glycopeptide coupling						glycopeptide	To reduce the amount of precious oligosaccharide that is required in the conventional SPPS method, an improved liquid phase glycopeptide coupling was also optimized in a good yield (46% over four steps).
37336068	3	19	gly	glycosylated	444:455	arg1	β-1,4-GlcNAc glycosylated wall teichoic acids				β-1,4-GlcNAc glycosylated wall teichoic acids						β-1,4-GlcNAc glycosylated wall teichoic acids (WTAs) derived from S. aureus are a new type of antigen that is closely associated with β-lactam resistance.
34452239	8	110	gly	glycopeptide	1669:1680	arg2	glycopeptide			glycopeptide						peptide, glycopeptide	The native peptide, glycopeptide, SD, and co-SD powders were comprehensively characterized, and exhibited distinct glass transitions (Tg) consistent with the amorphous glassy state formation with Tgs that are compatible with use in vivo.
36580234	4	17	gly	glycopeptides	549:561	arg2	The intact glycopeptides			The intact glycopeptides						glycopeptides	The intact glycopeptides of macrophages were enriched and analyzed using mass spectrometry (MS)-based glycoproteomic approaches, followed by the large-scale mapping of site-specific glycan structures via StrucGP.
31941975	3	39	gly	fucosylated	500:510	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Although many of biological and clinical studies of protein fucosylation by fucosyltransferases has been reported, structural classification of fucosylated N-glycoproteins such as core or outer isoforms remains a challenge.
31941975	3	96	gly	N-glycoproteins	512:526	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Although many of biological and clinical studies of protein fucosylation by fucosyltransferases has been reported, structural classification of fucosylated N-glycoproteins such as core or outer isoforms remains a challenge.
33403394	5	41	part_of	Fas	944:946	arg1	two Fas extracellular N-linkage sites	Fas		two Fas extracellular N-linkage sites		OGER	Site	Fas	P49327	sites	Mutations on two Fas extracellular N-linkage sites (118 and 136) for glycosylation impaired activation of Fas-mediated apoptosis during NK-92MI cytotoxicity.
33075613	4	60	gly	glycoproteins	642:654	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Dose delivery can be impeded by a matrix of N-linked glycoproteins and other polypeptides called the chorion.
35995381	11	110	part_of	thyroglobulin	1679:1691	arg1	the N-glycosylation sites	thyroglobulin		the N-glycosylation sites		PUBTATOR	Site	thyroglobulin	7038	sites	Therefore, a comprehensive analysis of the N-glycosylation sites of human thyroglobulin is essential to improve our understanding of the function of its N-glycans.
35373202	9	34	gly	glycosylation	1837:1849	arg1	this epitope			this epitope						epitope	In addition, in-cell analysis shows that the GalNAc-T4 isoform is the only isoform glycosylating the Thr of the immunogenic epitope PDTRP in vivo, which highlights the relevance of GalNAc-T4 in the glycosylation of this epitope.
35373202	9	55	gly	isoform	1714:1720	arg1	the Thr			the Thr						Thr	In addition, in-cell analysis shows that the GalNAc-T4 isoform is the only isoform glycosylating the Thr of the immunogenic epitope PDTRP in vivo, which highlights the relevance of GalNAc-T4 in the glycosylation of this epitope.
33287410	6	61	gly	trisialylated	1240:1252	arg1	triantennary trigalactosylated trisialylated glycans				triantennary trigalactosylated trisialylated glycans						We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease.
33287410	6	65	gly	monosialylated	1075:1088	arg1	biantennary digalactosylated monosialylated glycans				biantennary digalactosylated monosialylated glycans						We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease.
35380435	8	10	gly	glycopeptide	1731:1742	arg2	glycopeptide data			glycopeptide data						glycopeptide	In addition, our top-down analysis readily captured unexpected modifications (e.g., cysteinylation and N-terminal sequence variation) and low abundance, heavily glycosylated proteoforms that may be missed by using glycopeptide data alone.
36499311	1	47	gly	glycosylated	187:198	arg1	a highly glycosylated enzyme	a highly glycosylated enzyme				Fterm		enzyme			Rhodotorula dairenensis β-fructofuranosidase is a highly glycosylated enzyme with broad substrate specificity that catalyzes the synthesis of 6-kestose and a mixture of the three series of fructooligosaccharides (FOS), fructosylating a variety of carbohydrates and other molecules as alditols.
36499311	1	47	gly	glycosylated	187:198	arg1	Rhodotorula dairenensis β-fructofuranosidase	Rhodotorula dairenensis β-fructofuranosidase				Fterm		β-fructofuranosidase			Rhodotorula dairenensis β-fructofuranosidase is a highly glycosylated enzyme with broad substrate specificity that catalyzes the synthesis of 6-kestose and a mixture of the three series of fructooligosaccharides (FOS), fructosylating a variety of carbohydrates and other molecules as alditols.
35193013	8	51	gly	di-sialylated	1379:1391	arg1	predominantly mono- and di-sialylated N-glycans				predominantly mono- and di-sialylated N-glycans						In BALB/c mouse sera, we detected predominantly mono- and di-sialylated N-glycans with mostly N-Glycolylneuraminic acid (Neu5Gc) and only trace amounts of N-Acetyl neuraminic acid (Neu5Ac).
35995381	1	19	gly	glycosylation	167:179	arg2	many glycosylation sites			many glycosylation sites						sites	Human thyroglobulin (Tg), which has many glycosylation sites, is an essential protein produced by the human thyroid glands.
36870654	2	13	gly	glycosylation	284:296	arg1	a Family 1 carbohydrate-binding module	glycoprotein			a Family 1 carbohydrate-binding module	Fterm		glycoprotein			The present study addresses this knowledge gap by elucidating the links between the glycosylation patterns of a model glycoprotein, a Family 1 carbohydrate-binding module (TrCBM1), and the thermodynamic and structural properties of its binding to different carbohydrate substrates using isothermal titration calorimetry and computational simulation.
36870654	2	64	gly	glycoprotein	318:329	arg1	a Family 1 carbohydrate-binding module	glycoprotein			a Family 1 carbohydrate-binding module	Fterm		glycoprotein			The present study addresses this knowledge gap by elucidating the links between the glycosylation patterns of a model glycoprotein, a Family 1 carbohydrate-binding module (TrCBM1), and the thermodynamic and structural properties of its binding to different carbohydrate substrates using isothermal titration calorimetry and computational simulation.
34274643	2	44	gly	glycoprotein	374:385	arg1	Human alpha1-acid glycoprotein	Human alpha1-acid glycoprotein				Fterm		glycoprotein			Human alpha1-acid glycoprotein (AGP) was used as a model analyte due to its heterogeneous glycosylation resulting from variations in its degree of branching, fucosylation, and number of sialic acids.
34274643	2	83	gly	fucosylation	514:525	arg1	sialic acids				sialic acids						Human alpha1-acid glycoprotein (AGP) was used as a model analyte due to its heterogeneous glycosylation resulting from variations in its degree of branching, fucosylation, and number of sialic acids.
36968546	7	47	gly	O-glycopeptides	1157:1171	arg2	synthetic O-glycopeptides			synthetic O-glycopeptides						O-glycopeptides	We found that the sole incorporation of N-acetylgalactosamine did not impact furin activity in synthetic O-glycopeptides, but the presence of sialic acid reduced the furin rate by up to 65%.
33552045	3	77	gly	glycosylation	444:456	arg1	viral proteins	proteins		epitopes		Fterm		proteins		epitopes	In this perspective, we hypothesize that glycosylation of viral proteins and processed epitopes contribute to the T cell response to HIV.
34029329	4	95	part_of	gp120	660:664	arg1	1814 full length HIV-1 subtype C gp120 sequence	gp120		1814 full length HIV-1 subtype C gp120 sequence		PUBTATOR	Site	gp120	3700	sequence	A total of 1814 full length HIV-1 subtype C gp120 sequence from 37 countries were retrieved from Los Alamos National Laboratory HIV database (www.hiv.lanl.gov).
35022995	2	46	part_of	protein	216:222	arg1	a carboxy-terminal region	protein		a carboxy-terminal region		Fterm	Site	protein		region	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
36252735	1	29	gly	modified	165:172	arg3	The proteins AND glycans	The proteins			glycans	Fterm		proteins			The proteins in the cell membrane of the brain are modified by glycans in highly interactive regions.
36252735	1	30	gly	glycans	177:183	arg1	highly interactive regions			highly interactive regions	highly interactive regions		Site			regions	The proteins in the cell membrane of the brain are modified by glycans in highly interactive regions.
33997890	8	76	gly	glycosylation	1230:1242	arg1	site Thr393			site Thr393						site Thr393	The commercial product contained extensive N- and O-linked glycosylation as well as O-phosphorylation on site Thr393.
36324280	1	17	gly	N-glycosylation	181:195	arg2	four N-glycosylation sites			four N-glycosylation sites						sites	Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans.
36324280	1	60	gly	carrying	203:210	arg1	four N-glycosylation sites AND mainly complex type N-glycans			four N-glycosylation sites	mainly complex type N-glycans					sites	Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans.
35481895	7	5	gly	region	1306:1311	arg1	O-glycans			region	O-glycans					region	The S-layer protein is also extensively modified in the threonine-rich region near the C-terminus with O-glycans composed exclusively of hexoses.
35670884	4	4	gly	glycopeptide	779:790	arg2	its VCSNDNcfK glycopeptide			its VCSNDNcfK glycopeptide						glycopeptide	Site-specific mass spectrometry analysis demonstrated that the secreted protein acidic and rich in cysteine (SPARC), which binds collagen, contains a core-fucosylation site in its VCSNDNcfK glycopeptide.
35670884	4	23	gly	site	757:760	arg1	its VCSNDNcfK glycopeptide			its VCSNDNcfK glycopeptide	its VCSNDNcfK glycopeptide		Site			glycopeptide	Site-specific mass spectrometry analysis demonstrated that the secreted protein acidic and rich in cysteine (SPARC), which binds collagen, contains a core-fucosylation site in its VCSNDNcfK glycopeptide.
35344340	2	32	part_of	G	443:443	arg1	the immunoglobulin G (IgG) Fc CH2 domain	immunoglobulin G		the immunoglobulin G (IgG) Fc CH2 domain		Cterm	Site	immunoglobulin G		domain	We evaluated thermal and metabolic stabilities of antibody-drug conjugates (ADCs) with payloads attached to the C'E loop in the immunoglobulin G (IgG) Fc CH2 domain, comparing the glycosylated and aglycosylated Fc ADC variants.
35504880	6	35	gly	α-GalNAc-Ser	931:942	arg1	α-GalNAc-Ser peptides			Ser	α-GalNAc-Ser peptides					Ser	By imposing this 3D-arrangement on that fragment, characteristic of α-GalNAc-Ser peptides, C1GalT1 ensures broad glycosylation of both acceptor substrates.
33206215	2	26	part_of	proteins	390:397	arg1	prolines	proteins		prolines and lysines		Fterm	AminoAcid	proteins		prolines and lysines	The extracellular matrix (ECM) consists of (1) fibrous proteins, post-translationally modified (PTM) via N- and O-linked glycosylation, as well as hydroxylation on prolines and lysines, and (2) glycosaminoglycan-decorated proteoglycans.
37202422	6	3	gly	afucosylated	884:895	arg1	the afucosylated IgG N-glycan				the afucosylated IgG N-glycan						Upon binding, the elongated CDR3 loop of X0 undergoes a conformational shift to access the buried N-glycan and acts as a 'glycan sensor', forming hydrogen bonds with the afucosylated IgG N-glycan that would otherwise be sterically hindered by the presence of a core fucose residue.
35380345	6	10	gly	sialylated	852:861	arg1	sialylated, fucosylated and mannosylated N-glycans				sialylated, fucosylated and mannosylated N-glycans						The cells grown in HG showed a greater abundance of N-glycans when compared to LNG cells, without changes in the proportion of sialylated, fucosylated and mannosylated N-glycans.
36972173	3	55	part_of	epitopes	540:547	arg1	spike glycoproteins	glycoproteins		epitopes		Fterm	Site	glycoproteins		epitopes	This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins.
34853076	3	42	gly	glycosylation	374:386	arg2	a large N-linked glycosylation site			a large N-linked glycosylation site						site	Native C5a contains a large N-linked glycosylation site at Asn64, which accounts for up to 25% of its m.w. To date, the vast majority of published studies examining C5a are performed using Escherichia coli-generated recombinant C5a, which is readily available from numerous commercial suppliers, but lacks this glycosylation moiety.
34853076	3	42	gly	glycosylation	374:386	arg2	Asn64			Asn64						Asn64	Native C5a contains a large N-linked glycosylation site at Asn64, which accounts for up to 25% of its m.w. To date, the vast majority of published studies examining C5a are performed using Escherichia coli-generated recombinant C5a, which is readily available from numerous commercial suppliers, but lacks this glycosylation moiety.
36181511	4	65	gly	glycopeptide	916:927	arg2	a reasonable glycopeptide enrichment performance			a reasonable glycopeptide enrichment performance						glycopeptide	Exploration of capacity and recovery rate of cotton HILIC illustrated that 5mg cotton packed in a 200μL tip achieved a reasonable glycopeptide enrichment performance (~6% recovery) from ~0.5mg peptides.
35200645	7	8	part_of	sr-SPs	1391:1396	arg1	The chemical composition	SPs		The chemical composition		OGER	Site	SPs	P49903	position,	The chemical composition, FTIR and 1H NMR spectroscopic, and molecular weights of the sr-SPs from H. floresii were determined and discussed based on the anti-herpetic activity.
34407556	4	74	part_of	ASGR2	678:682	arg1	ASGR2 5' untranslated region	ASGR2		ASGR2 5' untranslated region		PUBTATOR	Site	ASGR2	433	region	PK parameters were evaluated in relation to ASGR2 5' untranslated region (5'UTR) polymorphisms, which were investigated by recombinant and white blood cell reverse transcription-polymerase chain reaction approaches.
37366623	9	4	part_of	B	1751:1751	arg1	the specific site	the envelope glycoprotein B (gB		the specific site		Cterm	Site	the envelope glycoprotein B (gB		site	Here, we show that an N-glycan shield on the specific site of the envelope glycoprotein B (gB) of HSV-1 mediates evasion from pooled γ-globulins derived from human blood both in cell cultures and mice.
34985300	2	20	gly	O-glycosylation	261:275	arg2	the O-glycosylation motif			the O-glycosylation motif						motif	O-glycosylation has been reported in this phylum with findings that include the O-glycosylation motif, the structure of the O-glycans in a few species, and an extensive O-glycoproteome analysis in Tannerella forsythia.
33609912	11	2	part_of	Fab	1688:1690	arg1	Fab and Fc fragments	Fab		Fab and Fc fragments		PUBTATOR	Site	Fab	2187	fragments	Different glycosylation profiles were also observed in Fab and Fc fragments from neutralizing and non-neutralizing IgG1, although the deglycosylated IgG1 lost its neutralizing activity.
33609912	11	56	part_of	IgG1	1748:1751	arg1	Fab and Fc fragments	IgG1		Fab and Fc fragments		OGER	Site	IgG1	P01857	fragments	Different glycosylation profiles were also observed in Fab and Fc fragments from neutralizing and non-neutralizing IgG1, although the deglycosylated IgG1 lost its neutralizing activity.
33609912	11	91	part_of	Fc	1696:1697	arg1	Fab and Fc fragments	Fc		Fab and Fc fragments		Cterm	Site	Fc		fragments	Different glycosylation profiles were also observed in Fab and Fc fragments from neutralizing and non-neutralizing IgG1, although the deglycosylated IgG1 lost its neutralizing activity.
35092134	2	67	gly	N-glycosylation	280:294	arg2	11 potential N-glycosylation sites			11 potential N-glycosylation sites						sites	MET contains 11 potential N-glycosylation sites, but the site-specific roles of these N-glycans have not been elucidated.
32602701	5	68	gly	glycosylated	676:687	arg1	Asn-229			Asn-229 and Asn-281						Asn-229 and Asn-281	Mass spectrometry confirmed that Asn-229 and Asn-281 were glycosylated, and substituting both Asn sites with Gln prevented myonectin secretion.
32417172	3	33	gly	glycosylated	401:412	arg1	nucleocytoplasmic proteins	nucleocytoplasmic proteins				Fterm		proteins			However, what nucleocytoplasmic proteins are O-GalNAc glycosylated and what the biological function of this modification in cells are still poorly understood.
31941975	0	19	gly	N-Glycoproteins	59:73	arg1	Outer Fucosylation	N-Glycoproteins			Outer Fucosylation	Fterm		N-Glycoproteins			Machine Learning Classifies Core and Outer Fucosylation of N-Glycoproteins Using Mass Spectrometry.
31941975	0	104	gly	Fucosylation	43:54	arg1	N-Glycoproteins	N-Glycoproteins				Fterm		N-Glycoproteins			Machine Learning Classifies Core and Outer Fucosylation of N-Glycoproteins Using Mass Spectrometry.
35616904	4	15	part_of	glycoproteins	730:742	arg1	1019 N-glycosites	glycoproteins		1019 N-glycosites		Fterm	Site	glycoproteins		N-glycosites	A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
32691273	2	96	part_of	proteins	442:449	arg1	the serine or threonine residues	proteins		the serine or threonine residues		Fterm	AminoAcid	proteins		threonine residues	O-GlcNAc transferase (OGT) catalyzes the attachment, while O-GlcNAcase (OGA) splits the GlcNAc molecules from the serine or threonine residues of the nuclear and cellular proteins.
36825496	3	18	gly	fucosylated	364:374	arg1	fucosylated fragments			fucosylated fragments						fragments	Here, using ion vibrational spectroscopy, we resolve the structure of fucosylated fragments of Lewis and blood group H antigen trisaccharides and we unveil the position and linkage of the fucose after migration.
36809652	4	95	gly	O-glycoprotein	614:627	arg1	another O-glycoprotein	another O-glycoprotein				Fterm		O-glycoprotein			Carbohydrate-dependent binding of galectin-1 to another O-glycoprotein, neuropilin-1 (NRP-1) on endothelial cells activates vascular endothelial growth factor receptor 2 (VEGFR2) and mitogen-activated protein kinase (MAPK) signaling.
34778211	13	32	gly	core-fucosylation	2250:2266	arg1	proteins	proteins				Fterm		proteins			These glycoproteomic results revealed that the knock-out of FUT8 not only influenced the core-fucosylation of proteins but also altered other glycosylation synthesis processes and changed the relative abundance of protein glycosylation.
34778211	13	91	gly	proteins	2271:2278	arg1	the core-fucosylation	proteins			the core-fucosylation	Fterm		proteins			These glycoproteomic results revealed that the knock-out of FUT8 not only influenced the core-fucosylation of proteins but also altered other glycosylation synthesis processes and changed the relative abundance of protein glycosylation.
35495330	0	77	part_of	hemagglutinin	13:25	arg1	Mannosylated hemagglutinin peptides	hemagglutinin		Mannosylated hemagglutinin peptides		Fterm	Site	hemagglutinin		peptides	Mannosylated hemagglutinin peptides bind cyanovirin-N independent of disulfide-bonds in complementary binding sites.
36181511	2	50	gly	glycopeptide	396:407	arg2	large-scale glycopeptide enrichment			large-scale glycopeptide enrichment						glycopeptide	Herein, we introduced a cotton hydrophilic interaction liquid chromatography (HILIC) approach for large-scale glycopeptide enrichment with 80% acetonitrile/1% trifluoroacetic acid as the optimal sample loading buffer.
36478308	7	82	gly	glycopeptides	1155:1167	arg2	previously detected glycopeptides			previously detected glycopeptides						glycopeptides	Glycopeptide mapping data processing and glycan classification was performed using Genedata Expressionist via a specialised workflow that used libraries of previously detected glycopeptides to greatly reduce processing time.
36892535	7	63	part_of	MUC1	1080:1083	arg1	the glycosylated MUC1 peptides	MUC1		the glycosylated MUC1 peptides		OGER	Site	MUC1	P15941	peptides	To decipher the molecular mechanism for the observed adhesion we investigated conformation of the glycosylated MUC1 peptides by NMR.
34452239	6	101	gly	glycopeptide	1324:1335	arg2	glycopeptide			glycopeptide						peptide and glycopeptide	Measurements of solubility and lipophilicity of raw Ang (1-7) and raw PNA5 using experimental and computational approaches confirmed that both the peptide and glycopeptide have high-water solubility and are amphipathic.
35532124	7	41	part_of	HDX	1116:1118	arg1	specific regions	HDX		specific regions		OGER	Site	HDX	Q7Z353	regions	The N-linker also decreases HDX in specific regions of A1 and increases thermal stability and the energy gap between its native state and an intermediate state, which is observed in urea-induced unfolding.
35532124	7	57	part_of	A1	1143:1144	arg1	specific regions	A1		specific regions		PUBTATOR	Site	A1	28881	regions	The N-linker also decreases HDX in specific regions of A1 and increases thermal stability and the energy gap between its native state and an intermediate state, which is observed in urea-induced unfolding.
35597280	8	4	part_of	TSR3	1488:1491	arg1	the TSR3 domain	TSR3		the TSR3 domain		PUBTATOR	Site	TSR3	115939	domain	Here, we conducted molecular dynamics (MD) simulations and provide crystallographic and NMR evidence that the Glucose-Fucose disaccharide interacts with specific amino acids in the TSR3 domain in thrombospondin-1 that are within proximity to the O-fucosylation modification site resulting in protection of a nearby disulfide bond.
35597280	8	7	part_of	domain	1493:1498	arg1	thrombospondin-1	thrombospondin-1		domain		PUBTATOR	Site	thrombospondin-1	7057	domain	Here, we conducted molecular dynamics (MD) simulations and provide crystallographic and NMR evidence that the Glucose-Fucose disaccharide interacts with specific amino acids in the TSR3 domain in thrombospondin-1 that are within proximity to the O-fucosylation modification site resulting in protection of a nearby disulfide bond.
36252443	2	87	gly	glycoproteins	283:295	arg1	Released N-glycans	glycoproteins			Released N-glycans	Fterm		glycoproteins			Released N-glycans from milk glycoproteins act as growth substrates for infant-associated bifidobacteria, which are key members of the breastfed infant's gut.
36493594	0	65	gly	glycoproteins	142:154	arg1	mammalian glycoproteins	mammalian glycoproteins				Fterm		glycoproteins			Peptide-N-glycosidase F or A treatment and procainamide-labeling for identification and quantification of N-glycans in two types of mammalian glycoproteins using UPLC and LC-MS/MS.
36014516	4	11	gly	fragment	758:765	arg1	the polysaccharide fragment Mn				the polysaccharide fragment Mn						Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 106~9.56 × 106 Da, and the FT-IR absorption peak of 892 cm-1 can be used as the quality control markers of SPs.
36014516	4	11	gly	fragment	758:765	arg1	Two monosaccharides				Two monosaccharides						Two monosaccharides (rhamnose and galactose), the polysaccharide fragment Mn = 8.67 × 106~9.56 × 106 Da, and the FT-IR absorption peak of 892 cm-1 can be used as the quality control markers of SPs.
32636304	7	24	part_of	site	1382:1385	arg1	the protein	protein		site		Fterm	Site	protein		site	This multivalent mode of binding was further strengthened by interactions of core mannosyl residues with a secondary conserved site on the protein, leading to an exponential increase in affinity.
36068283	3	60	gly	glycopeptides	512:524	arg2	isobaric glycopeptides			isobaric glycopeptides						glycopeptides	Using parallel sequencing of isobaric glycopeptides and isomeric glycans that were separated by reversed-phase and porous graphitic carbon LC, we report a highly sensitive LC MS/MS method for the comprehensive characterization of low-abundance non-human glycans and their closely related structural isomers.
36211377	7	27	gly	sialylated	1195:1204	arg1	sialylated glycans				sialylated glycans						In the follow-up period, the increase in glycans with bisecting GlcNAc and the decrease in sialylated glycans were observed.
35304921	13	63	gly	Glycosylation	1789:1801	arg1	α5 β1 integrin	α5 β1 integrin				Fterm		integrin			SIGNIFICANCE Glycosylation of α5 β1 integrin has been implicated in multiple aspects of integrin function and structure.
36436856	6	16	gly	fucosylated	755:765	arg1	fucosylated N-glycans				fucosylated N-glycans						In GDM milk, fucosylated N-glycans present higher proportion, whereas the proportion of sialylated O-glycans were lower.
36436856	6	41	gly	sialylated	830:839	arg1	sialylated O-glycans				sialylated O-glycans						In GDM milk, fucosylated N-glycans present higher proportion, whereas the proportion of sialylated O-glycans were lower.
35211008	12	36	gly	N-glycosylation	2157:2171	arg1	β1 adrenergic receptors	β1 adrenergic receptors				Fterm		receptors			Decreased N-glycosylation of β1 adrenergic receptors will downregulate the cAMP/PKA signal pathway and inhibit myocardial excitation and contraction coupling.
36959936	2	86	gly	glycoproteins	322:334	arg1	viral glycoproteins	viral glycoproteins				Fterm		glycoproteins			In reality, however, differences in the plant biosynthetic machinery, compared to mammalian cells, can complicate the production of viral glycoproteins.
33176830	4	49	gly	N-glycosylation	635:649	arg2	non-canonical N-glycosylation motifs			non-canonical N-glycosylation motifs						motifs	Here, we explored the functional role of non-canonical N-glycosylation motifs in the conformation N-X-C based on site directed mutagenesis.
34199200	2	25	gly	glycoproteins	582:594	arg1	surface-expressed glycoproteins	surface-expressed glycoproteins				Fterm		glycoproteins			Herein, we investigated the binding of cyanovirin-N (CV-N) to surface-expressed glycoproteins such as those of human immunodeficiency virus (HIV) gp120, hemagglutinin (HA), and Ebola (GP)1,2 and compared their binding affinities with the binding response to the trimer-folded gp140 using surface plasmon resonance (SPR).
33712585	5	26	part_of	Ca2+-binding	721:732	arg1	a Ca2+-binding site	Ca2		a Ca2+-binding site		OGER	Site	Ca2	P00918	site	The 1.6 Å X-ray structure of the homodimeric A. fumigatus protein reveals unique properties including N-linked glycosylation and a Ca2+-binding site whose occupancy regulates activity.
35766466	4	48	gly	fucosylated	769:779	arg1	target core fucosylated N-glycans				target core fucosylated N-glycans						Enzymes that have previously been utilized to cleave N-glycans include peptide-N-glycosidase F (PNGase F) to target N-glycans indiscriminately and endoglycosidase F3 (Endo F3) to target core fucosylated N-glycans.
35943155	13	76	gly	oligosaccharides	2176:2191	arg1	cell surface glycoconjugate receptors	receptors			oligosaccharides	Fterm		receptors			IMPORTANCE α-l-Fucosyl residues are frequently present in many relevant glycans, such as human milk oligosaccharides (HMOs), histo-blood group antigens (HBGAs), and epitopes on cell surface glycoconjugate receptors.
37341462	4	80	gly	O-glycopeptides	791:805	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	In this study, we found that the Y-ion patterns were also frequently observed in the spectra of O-glycopeptides, and a special search approach is presented to identify O-glycopeptides by utilizing the Y-ion patterns.
37341462	4	82	gly	O-glycopeptides	719:733	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	In this study, we found that the Y-ion patterns were also frequently observed in the spectra of O-glycopeptides, and a special search approach is presented to identify O-glycopeptides by utilizing the Y-ion patterns.
36210615	3	61	gly	glycosylation	635:647	arg2	several glycosylation sites			several glycosylation sites						sites	Considering that metalloproteases and serine proteases are abundant components of Bothrops venoms and essential in the envenomation process, and that these enzymes contain several glycosylation sites, the role of sialic acid in venom proteolytic activity was evaluated.
32783937	5	33	part_of	gut	980:982	arg1	gut microbial compositions	gut		gut microbial compositions		PUBTATOR	Site	gut	110006	positions,	Intestinal epithelial ablation of OGT in mice not only causes L cell hyperplasia and increased secretion of glucagon-like peptide 1 (GLP-1) but also disrupts gut microbial compositions, which notably contributes to decreased weight gain and improved glycemic control.
33904933	8	91	part_of	epitope	1589:1595	arg1	annexin A2	annexin A2		epitope		PUBTATOR	Site	annexin A2	302	epitope	Our findings indicate that 12G5A identified a cancer-associated glycosylation epitope on annexin A2, whose expression was related to unfavorable colorectal cancer behavior.
36136114	7	13	gly	glycopeptide	1152:1163	arg2	glycopeptide ionization			glycopeptide ionization						glycopeptide	Using conventional reversed-phase LC-MS methods, the different glycoforms at a given glycosite elute over a narrow retention time window, and glycopeptide ionization is suppressed by co-eluting non-modified peptides.
36136114	7	45	gly	glycoforms	1073:1082	arg2	a given glycosite			a given glycosite						glycosite	Using conventional reversed-phase LC-MS methods, the different glycoforms at a given glycosite elute over a narrow retention time window, and glycopeptide ionization is suppressed by co-eluting non-modified peptides.
36136114	7	48	gly	glycosite	1095:1103	arg2	a given glycosite			a given glycosite						glycosite	Using conventional reversed-phase LC-MS methods, the different glycoforms at a given glycosite elute over a narrow retention time window, and glycopeptide ionization is suppressed by co-eluting non-modified peptides.
35802832	2	13	gly	glycomacropeptide	371:387	arg1	O-glycans			glycomacropeptide	O-glycans					glycomacropeptide	In this study, O-glycans of casein glycomacropeptide from bovine and caprine whey powder were qualitatively and quantitatively analyzed by LC-UV-ESI-MS/MS, and their immune activities and regulatory mechanisms were compared.
34960798	3	106	part_of	env	744:746	arg1	gag and env sequences	env		gag and env sequences		PUBTATOR	Site	env	100616444	sequences	On the basis of gag and env sequences, Polish SRLVs were assigned to the subtypes B2, A5, A12, and A17.
34960798	3	108	part_of	gag	736:738	arg1	gag and env sequences	gag		gag and env sequences		PUBTATOR	Site	gag	155030	sequences	On the basis of gag and env sequences, Polish SRLVs were assigned to the subtypes B2, A5, A12, and A17.
37028137	5	66	gly	composition	781:791	arg1	non-reducing termini			non-reducing termini						termini	However, the individual function of the two N-glycans and the effects of N-glycan composition (size, structure, and non-reducing termini) on DET-type reactions are still unclear.
36990248	9	41	gly	T-synthase	1605:1614	arg1	O-glycan profile	T-synthase			O-glycan profile	Fterm		T-synthase			Altogether, our results elucidated the O-glycan profile and function of T-synthase in the silkworm.
34029329	11	67	part_of	gp120	1761:1765	arg1	global intra-subtype C gp120 sequences	gp120		global intra-subtype C gp120 sequences		PUBTATOR	Site	gp120	3700	sequences	Our findings highlight that distinctly evolving clusters within global intra-subtype C gp120 sequences are likely to influence the disparate region-specific sensitivity of circulating HIV-1 subtype C to bnAbs.
34691043	7	4	gly	hyperglycosylated	877:893	arg1	the hyperglycosylated HA trimer	the hyperglycosylated HA trimer				Fterm		trimer			Here, we asked whether cysteine stabilization of the hyperglycosylated HA trimer could reverse this immunodominance by preventing access to the interface epitope and focus responses to the HA receptor binding site (RBS).
36399685	3	41	gly	glycoproteins	474:486	arg1	asparagine-linked (N-linked) glycoproteins	asparagine-linked (N-linked) glycoproteins				Fterm		glycoproteins			To address this gap, we developed a set of complementary methods for producing stalled ribosome complexes that displayed asparagine-linked (N-linked) glycoproteins in conformations amenable to downstream functional and glycostructural interrogation.
37267309	7	4	part_of	protein	1161:1167	arg1	a periplasmic glycosyl hydrolase family GH17 domain	protein		a periplasmic glycosyl hydrolase family GH17 domain		Fterm	Site	protein		domain	In silico analysis, including sequence comparisons, secondary structure and topology predictions, and protein structural modelling, revealed a two-domain transmembrane ovoid-like structure for the Orphan protein with a periplasmic glycosyl hydrolase family GH17 domain linked via a transmembrane region to a cytoplasmic glycosyltransferase family GT2 domain.
35931292	7	68	gly	glycosylated	995:1006	arg1	0.41-0.66 μM Trolox/mg protein	0.41-0.66 μM Trolox/mg protein				Fterm		protein			Antioxidant capacity of glycosylated products (0.41-0.66 μM Trolox/mg protein) was markedly greater than that of original protein (0.06 μM Trolox/mg protein) due to the formation of brown polymers with antioxidant activity.
36648436	1	6	gly	sialylated	215:224	arg1	STn	STn			its sialylated form	PUBTATOR		STn	1917		In epithelial cancers, truncated O-glycans, such as the Thomson-nouveau antigen (Tn) and its sialylated form (STn), are up-regulated on the cell surface and associated with poor prognosis and immunological escape.
36181511	1	1	gly	glycopeptides	190:202	arg2	glycopeptides			glycopeptides						glycopeptides	Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples.
36181511	1	10	gly	glycopeptide	91:102	arg2	Selecting proper and efficient glycopeptide enrichment approaches			Selecting proper and efficient glycopeptide enrichment approaches						glycopeptide	Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples.
34325731	12	17	part_of	FOXF1	2205:2209	arg1	the FOXF1 coding region	FOXF1		the FOXF1 coding region		OGER	Site	FOXF1	Q12946	region	Hypermethylation at the 60 kb FOXF1 enhancer might contribute to FOXF1 haploinsufficiency caused by heterozygous mutations in the FOXF1 coding region.
33310751	8	32	part_of	enzymes	1132:1138	arg1	the same conserved catalytic domain	enzymes		the same conserved catalytic domain		Fterm	Site	enzymes		domain	Most bacterial OGAs are secreted enzymes with the same conserved catalytic domain as human OGAs.
32357974	7	69	gly	glycoproteins	1463:1475	arg1	misfolded and retrotranslocated glycoproteins	misfolded and retrotranslocated glycoproteins				Fterm		glycoproteins			Chemical inhibition of Peptide:N-Glycanase (PNGase), the endoglycosidase responsible for the removal of glycans from misfolded and retrotranslocated glycoproteins, greatly reduced presentation of this subset of deamidated HLA-bound peptides.
34985300	13	117	gly	O-glycosylated	2273:2286	arg1	most proteins	most proteins				Fterm		proteins			It was found that most proteins exposed to the periplasm were O-glycosylated; however, the abundant surface exposed proteins were not.
33952641	4	2	gly	glycoproteins	525:537	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			To more globally identify glycoproteins that are differentially expressed on the surface of HHV6A-infected cells, we performed cell surface capture of N-linked glycoproteins present on the surface of T cells infected with HHV6A, and compared these to proteins present on the surface of uninfected T cells.
33952641	4	11	gly	glycoproteins	659:671	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			To more globally identify glycoproteins that are differentially expressed on the surface of HHV6A-infected cells, we performed cell surface capture of N-linked glycoproteins present on the surface of T cells infected with HHV6A, and compared these to proteins present on the surface of uninfected T cells.
36716113	8	106	gly	pro-inflammatory/anti-inflammatory	1792:1825	arg1	more balanced pro-inflammatory/anti-inflammatory glycan profiles			pro	more balanced pro-inflammatory/anti-inflammatory glycan profiles					pro	CONCLUSION Patients with antibiotic-responsive LA had Bb-IgG1 antibodies with more balanced pro-inflammatory/anti-inflammatory glycan profiles, whereas patients with antibiotic-refractory LA had Bb-IgG1 antibodies with maximal, minimally opposed, pro-inflammatory glycan profiles.
36716113	8	126	gly	maximal	1919:1925	arg1	pro-inflammatory			pro-inflammatory						pro	CONCLUSION Patients with antibiotic-responsive LA had Bb-IgG1 antibodies with more balanced pro-inflammatory/anti-inflammatory glycan profiles, whereas patients with antibiotic-refractory LA had Bb-IgG1 antibodies with maximal, minimally opposed, pro-inflammatory glycan profiles.
36049519	9	61	gly	glycosylated	1478:1489	arg1	the glycosylated residue			the glycosylated residue						residue	The enzyme catalyzes hydrolysis of the bond immediately N-terminal to the glycosylated residue.
31941975	10	57	gly	fucosylated	1877:1887	arg1	fucosylated N-glycopeptides			fucosylated N-glycopeptides						N-glycopeptides	Thus, the machine learning methods can be combined with MS/MS to distinguish between different isoforms of fucosylated N-glycopeptides.
31941975	10	106	gly	N-glycopeptides	1889:1903	arg2	fucosylated N-glycopeptides			fucosylated N-glycopeptides						N-glycopeptides	Thus, the machine learning methods can be combined with MS/MS to distinguish between different isoforms of fucosylated N-glycopeptides.
31657490	7	34	gly	glycopeptides	1233:1245	arg2	glycopeptides			glycopeptides						glycopeptides	In addition, the proposed material showed good performance in the enrichment of glycopeptides from complex biosamples; 56 glycopeptides were detected from 2 μL of human serum using MALDI-TOFMS.
31657490	7	87	gly	glycopeptides	1275:1287	arg2	56 glycopeptides			56 glycopeptides						glycopeptides	In addition, the proposed material showed good performance in the enrichment of glycopeptides from complex biosamples; 56 glycopeptides were detected from 2 μL of human serum using MALDI-TOFMS.
35848837	3	51	gly	glycopeptides	467:479	arg2	glycopeptides			glycopeptides						glycopeptides	A common method for glycan analysis is to employ exoglycosidase cleavage to release N-linked glycans from glycoproteins or glycopeptides using Peptide-N-Glycosidase F (PNGase F).
35848837	3	58	gly	glycoproteins	450:462	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A common method for glycan analysis is to employ exoglycosidase cleavage to release N-linked glycans from glycoproteins or glycopeptides using Peptide-N-Glycosidase F (PNGase F).
37331167	8	40	gly	epitope	1212:1218	arg1	α-1,3 mannose				α-1,3 mannose						These results demonstrated that α-1,3 mannose was an critical component of glycan related epitope.
36136114	6	7	gly	glycoprotein	980:991	arg1	glycoprotein quality control	glycoprotein quality control				Fterm		glycoprotein			Because of the inherent heterogeneity of glycosylation, it is necessary to assign glycan structural detail for glycoprotein quality control.
36585837	13	56	gly	glycosylated	1766:1777	arg1	glycosylated milk proteins	glycosylated milk proteins				Fterm		proteins			These data are described and the relevance of glycosylated milk proteins in neonate development, such as protection against pathogens is discussed.
34274480	9	17	part_of	C1q	1179:1181	arg1	21 residues	C1q		21 residues		OGER	Site	C1q		residues	The deduced CBLN1 protein contains a putative signal sequence of 21 residues, two conserved cysteine residues, and C1q domain.
34274480	9	17	part_of	C1q	1179:1181	arg1	C1q domain	C1q		C1q domain		OGER	Site	C1q		domain	The deduced CBLN1 protein contains a putative signal sequence of 21 residues, two conserved cysteine residues, and C1q domain.
34274480	9	47	part_of	contains	1090:1097	arg1	The deduced CBLN1 protein AND 21 residues	The deduced CBLN1 protein		21 residues		PUBTATOR	Site	CBLN1 protein	869	residues	The deduced CBLN1 protein contains a putative signal sequence of 21 residues, two conserved cysteine residues, and C1q domain.
34274480	9	47	part_of	contains	1090:1097	arg1	The deduced CBLN1 protein AND a putative signal sequence	The deduced CBLN1 protein		a putative signal sequence		PUBTATOR	Site	CBLN1 protein	869	sequence	The deduced CBLN1 protein contains a putative signal sequence of 21 residues, two conserved cysteine residues, and C1q domain.
34274480	9	47	part_of	contains	1090:1097	arg1	The deduced CBLN1 protein AND C1q domain	The deduced CBLN1 protein		C1q domain		PUBTATOR	Site	CBLN1 protein	869	domain	The deduced CBLN1 protein contains a putative signal sequence of 21 residues, two conserved cysteine residues, and C1q domain.
37341462	7	51	gly	glycopeptide	1335:1346	arg2	15.4%-199.0% more O-glycopeptide-spectrum matches (OGPSMs) and 19.6%-107.1% more glycopeptide sequence identifications			15.4%-199.0% more O-glycopeptide-spectrum matches (OGPSMs) and 19.6%-107.1% more glycopeptide sequence identifications						glycopeptide	The new search strategy was applied to search a human serum data set, and 15.4%-199.0% more O-glycopeptide-spectrum matches (OGPSMs) and 19.6%-107.1% more glycopeptide sequence identifications than other state-of-the-art software tools were observed.
32691273	2	113	gly	residues	405:412	arg1	the GlcNAc molecules			threonine residues	the GlcNAc molecules					threonine residues	O-GlcNAc transferase (OGT) catalyzes the attachment, while O-GlcNAcase (OGA) splits the GlcNAc molecules from the serine or threonine residues of the nuclear and cellular proteins.
36056620	6	60	gly	glycosylation	1110:1122	arg2	N6 glycosylation motifs			N6 glycosylation motifs						motifs	Further reverse genetic studies of acquired E1/E2 substitutions identified positions 418 and 532 in the N1 and N6 glycosylation motifs, localizing to adjacent E2 areas, as key regulators of changes of the E1/E2 conformational state, which governed viral sensitivity to nAb.
37287875	0	6	gly	N-glycopeptides	19:33	arg2	intact N-glycopeptides			intact N-glycopeptides						N-glycopeptides	Identifying intact N-glycopeptides from tandem mass spectrometry data using StrucGP.
35219398	3	41	part_of	CEACAM1	462:468	arg1	CEACAM1 N-terminal Ig-like domain	CEACAM1		CEACAM1 N-terminal Ig-like domain		PUBTATOR	Site	CEACAM1	P13688	domain	Two putative dimer conformations involving different CEACAM1 N-terminal Ig-like domain (CCM1) protein faces (ABED and GFCC'C″) were identified from crystal structures.
36329887	1	70	gly	glycoprotein	194:205	arg1	an α/β heterodimeric glycoprotein hormone	an α/β heterodimeric glycoprotein hormone				Fterm		glycoprotein			Follicle-stimulating hormone (FSH), an α/β heterodimeric glycoprotein hormone, consists of functionally significant variants resulting from the presence or absence of either one of two FSHβ subunit N-glycans.
36269466	10	14	gly	glycoproteins	1495:1507	arg1	misfolded glycoproteins	misfolded glycoproteins				Fterm		glycoproteins			This hints at the accumulation of misfolded glycoproteins in the infected plants, likely because of endoplasmic reticulum (ER) stress.
35871410	8	89	gly	O-glycan	1293:1300	arg1	the Δmnt1 strain	strain			O-glycan	Fterm		strain			In this study, we elucidated the structure of the Mnt1 reaction product, the structure of O-glycan in the Δmnt1 strain.
31657490	2	29	gly	glycopeptides	416:428	arg2	glycopeptides			glycopeptides						glycopeptides	Therefore, selective enrichment of glycopeptides before mass spectrometry has turned into an urgent problem to be resolved.
37316505	0	57	gly	glycosylation	48:60	arg1	the stalk region			region						region	Intact mass analysis reveals the novel O-linked glycosylation on the stalk region of PD-1 protein.
34864901	0	0	gly	proteins	59:66	arg1	glycan shielding	proteins			glycan shielding	Fterm		proteins			GLYCO: a tool to quantify glycan shielding of glycosylated proteins.
34864901	0	5	gly	glycosylated	46:57	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			GLYCO: a tool to quantify glycan shielding of glycosylated proteins.
36233110	4	56	gly	N-glycosylation	923:937	arg2	all five N-glycosylation sites			all five N-glycosylation sites						sites	Three aflibercept lots showed a high degree of similarity in glycan composition, fucosylation level, sialylation level, and branching, when all five N-glycosylation sites were assessed together as a group.
35598016	2	50	gly	machinery	391:399	arg1	the thermophilic Caldicoprobacter algeriensis TH7C1T strain	strain			machinery	Fterm		strain			The aim of this study was to identify protein encoding genes including CAZymes in order to understand glycans-degrading machinery in the thermophilic Caldicoprobacter algeriensis TH7C1T strain.
36089065	0	40	gly	N-glycoprotein	30:43	arg1	Sequential in vitro enzymatic N-glycoprotein modification	Sequential in vitro enzymatic N-glycoprotein modification				Fterm		N-glycoprotein			Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing.
36827096	4	9	gly	glycoprotein	603:614	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			GYL is a glycoprotein containing two N-glycosylation sites per subunit.
36827096	4	56	gly	N-glycosylation	631:645	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	GYL is a glycoprotein containing two N-glycosylation sites per subunit.
34186233	1	67	part_of	proteins	400:407	arg1	serine/threonine residues	proteins		serine/threonine residues		Fterm	Site	proteins		residues	O-GlcNAcylation is a dynamic post-translational modification mediated by O-linked β-N-acetylglucosamine transferase (OGT) and O-GlcNAc hydrolase (OGA), that adds or removes a single β-N-acetylglucosamine (GlcNAc) moiety to or from serine/threonine residues of nucleocytosolic and mitochondrial proteins, respectively.
37258966	1	22	gly	glycoproteins	320:332	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycosylation is a common posttranslational modification of proteins and refers to the covalent addition of glycans, chains of polysaccharides, onto proteins producing glycoproteins.
34735575	1	16	gly	sites	343:347	arg1	glycans			sites	glycans					sites	Extensive glycosylation of the spike protein of severe acute respiratory syndrome coronavirus 2 virus not only shields the major part of it from host immune responses, but glycans at specific sites also act on its conformation dynamics and contribute to efficient host receptor binding, and hence infectivity.
35495330	6	75	gly	glycosylation	1457:1469	arg2	a naturally found N-linked glycosylation site			a naturally found N-linked glycosylation site						site	Thus, mono- and dimannosylated peptides with N-terminal cysteine facilitated site-specific interactions with HA peptides, mimicking a naturally found N-linked glycosylation site on the HA head domain.
35495330	6	88	gly	dimannosylated	1314:1327	arg1	mono- and dimannosylated peptides			mono- and dimannosylated peptides						peptides	Thus, mono- and dimannosylated peptides with N-terminal cysteine facilitated site-specific interactions with HA peptides, mimicking a naturally found N-linked glycosylation site on the HA head domain.
35854001	0	22	part_of	lectin	15:20	arg1	a lectin domain	lectin		a lectin domain		Fterm	Site	lectin	269181	domain	Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).
34726173	4	43	part_of	SDR	1117:1119	arg1	glycosylated SDR peptides	SDR		glycosylated SDR peptides		PUBTATOR	Site	SDR	8436	peptides	This study reports the crystal structures of SdgB and SdgA from S. aureus as well as multiple structures of SdgB in complex with its substrates (for example UDP, N-acetylglucosamine or SDR peptides), products (glycosylated SDR peptides) or phosphate ions.
34726173	4	85	part_of	SDR	1079:1081	arg1	SDR peptides	SDR		SDR peptides		PUBTATOR	Site	SDR	8436	peptides	This study reports the crystal structures of SdgB and SdgA from S. aureus as well as multiple structures of SdgB in complex with its substrates (for example UDP, N-acetylglucosamine or SDR peptides), products (glycosylated SDR peptides) or phosphate ions.
36385894	10	60	gly	glycopeptide	1960:1971	arg2	glycopeptide biomarkers			glycopeptide biomarkers						glycopeptide	This report represents the first serum glycan and glycopeptide biomarkers of Philippine lung cancer patients, further demonstrating the utility of mass spectrometry-based glycomic and glycoproteomic methods.
34726173	4	18	gly	glycosylated	1104:1115	arg1	glycosylated SDR peptides			glycosylated SDR peptides						peptides	This study reports the crystal structures of SdgB and SdgA from S. aureus as well as multiple structures of SdgB in complex with its substrates (for example UDP, N-acetylglucosamine or SDR peptides), products (glycosylated SDR peptides) or phosphate ions.
36988338	5	7	gly	proteins	737:744	arg1	polysaccharides	proteins			polysaccharides	Fterm		proteins			Although the functional groups of polysaccharides and proteins were confirmed by infrared spectroscopy; the presence of proteins couldn't be detected by SDS-PAGE and UV spectroscopy.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	several glycoproteins	several glycoproteins				Fterm		glycoproteins			The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	metalloprotease	metalloprotease				Fterm		metalloprotease			The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
35662639	5	70	gly	glycoproteins	1332:1344	arg1	serine protease	serine protease				Fterm		protease			The lectin capture strategy generated venom fractions enriched with several glycoproteins, including metalloprotease, serine protease, and L- amino acid oxidase, in addition to various types of low abundant enzymes.
36903897	1	56	gly	glycoprotein	197:208	arg1	the hydroxyproline-rich glycoprotein (HRGP) superfamily	the hydroxyproline-rich glycoprotein (HRGP) superfamily				Fterm		glycoprotein			Arabinogalactan-proteins (AGPs) are members of the hydroxyproline-rich glycoprotein (HRGP) superfamily.
36585837	10	32	gly	glycosylation	1449:1461	arg2	glycosylation sites			glycosylation sites						sites	Glycoproteins were found across all three phases of swine milk production and varied by number of glycosylation sites (1 to 14) and in abundance and distribution between colostrum, transitional and mature milk.
35987426	3	96	gly	glycoprotein	485:496	arg1	the glycoprotein pattern	the glycoprotein pattern				Fterm		glycoprotein			The aim of the present study was to examine the glycoprotein pattern of the horse urinary bladder urothelium by lectin histochemistry.
35865907	2	95	gly	polysaccharides	482:496	arg1	shorter sequences			shorter sequences	shorter sequences		Site			sequences	Reported here is an efficient, stereoselective and orthogonal one-pot synthesis of a tetradecasaccharide and shorter sequences from Lentinus giganteus polysaccharides with antitumor activities.
35166741	1	29	gly	glycoproteins	246:258	arg1	natural glycoproteins	natural glycoproteins				Fterm		glycoproteins			The synthesis of a sufficient amount of homogeneous glycoprotein is of great interest because natural glycoproteins show considerable heterogeneity in oligosaccharide structures, making the studies on glycan structure-function relationship difficult.
35166741	1	51	gly	glycoprotein	196:207	arg1	homogeneous glycoprotein	homogeneous glycoprotein				Fterm		glycoprotein			The synthesis of a sufficient amount of homogeneous glycoprotein is of great interest because natural glycoproteins show considerable heterogeneity in oligosaccharide structures, making the studies on glycan structure-function relationship difficult.
36014368	8	6	gly	N-glycosylation	1217:1231	arg2	the 22 predicted N-glycosylation sites			the 22 predicted N-glycosylation sites						sites	We show that 20 among the 22 predicted N-glycosylation sites are dominated by complex plant N-glycans and one carries oligomannoses.
36870092	7	8	gly	N-glycopeptides	935:949	arg2	The intact N-glycopeptides			The intact N-glycopeptides						N-glycopeptides	The intact N-glycopeptides and differentially expressed intact N-glycopeptides (DEGPs) were determined and quantified via intact N-glycopeptide search engine GPSeeker.
36870092	7	56	gly	N-glycopeptides	987:1001	arg2	differentially expressed intact N-glycopeptides			differentially expressed intact N-glycopeptides						N-glycopeptides	The intact N-glycopeptides and differentially expressed intact N-glycopeptides (DEGPs) were determined and quantified via intact N-glycopeptide search engine GPSeeker.
36870092	7	75	gly	N-glycopeptide	1053:1066	arg2	intact N-glycopeptide search engine			intact N-glycopeptide search engine						N-glycopeptide	The intact N-glycopeptides and differentially expressed intact N-glycopeptides (DEGPs) were determined and quantified via intact N-glycopeptide search engine GPSeeker.
35782731	6	16	part_of	region	860:865	arg1	the glycoprotein	glycoprotein		region		Fterm	Site	glycoprotein		region	Since the mentioned central region in the glycoprotein is not surrounded by glycans and is close to the heme, it is easily approachable to the solvent and substrate.
36781790	1	36	gly	glycopeptides	233:245	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Mass spectrometry-driven glycomics and glycoproteomics, the system-wide profiling of detached glycans and intact glycopeptides from biological samples, respectively, are powerful approaches to interrogate the heterogenous glycoproteome.
35945033	4	118	gly	glycoproteins	1130:1142	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Furthermore, liquid chromatography-mass spectrometry (LC-MS) workflows, such as hydrogen deuterium exchange mass spectrometry (HDX-MS), require a highly efficient enzymatic release of N-glycans at low pH values to facilitate the comprehensive structural analysis of glycoproteins.
37354227	5	16	gly	glycosylation	1008:1020	arg1	the spike protein	protein		sites		Fterm		protein		sites	Our data reveal that the amino acid substitutions and deletions between variants impact the abundance and type of glycans on glycosylation sites of the spike protein.
36385894	1	80	gly	glycosylation	239:251	arg1	secreted proteins	secreted proteins				Fterm		proteins			Aberrant glycosylation has been extensively reported in cancer, with fundamental changes in the glycosylation patterns of cell-surface and secreted proteins largely occurring during cancer progression.
36775128	6	20	gly	glycosylation	835:847	arg1	these proteins	these proteins				Fterm		proteins			We examine the glycosylation states of these proteins using a combination of antibody-enabled and ZIC-HILIC enrichment techniques, to demonstrate that these proteins are modified with C-, O-, and N-linked glycans.
36775128	6	43	gly	modified	990:997	arg1	these proteins AND N-linked glycans	these proteins			N-linked glycans	Fterm		proteins			We examine the glycosylation states of these proteins using a combination of antibody-enabled and ZIC-HILIC enrichment techniques, to demonstrate that these proteins are modified with C-, O-, and N-linked glycans.
33161144	1	25	gly	glycoproteins	214:226	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			The N-linked glycosylation pattern is an important quality attribute of therapeutic glycoproteins.
36745435	3	4	part_of	residues	504:511	arg1	LAM	LAM		residues		OGER	Site	LAM		residues	Arabinofuranose (Araf)residues in LAM and AG are connected either via α-1,2-trans linkages that are synthetically straightforward to install or the more challenging β-(1,2-cis) linkages.
36745435	3	12	part_of	Araf	499:502	arg1	Arabinofuranose (Araf)residues	Arabinofuranose (Araf		Arabinofuranose (Araf)residues		OGER	Site	Arabinofuranose (Araf	P10398	residues	Arabinofuranose (Araf)residues in LAM and AG are connected either via α-1,2-trans linkages that are synthetically straightforward to install or the more challenging β-(1,2-cis) linkages.
36745435	3	15	part_of	Arabinofuranose	482:496	arg1	Arabinofuranose (Araf)residues	Arabinofuranose (Araf		Arabinofuranose (Araf)residues		OGER	Site	Arabinofuranose (Araf	P10398	residues	Arabinofuranose (Araf)residues in LAM and AG are connected either via α-1,2-trans linkages that are synthetically straightforward to install or the more challenging β-(1,2-cis) linkages.
36985724	4	2	gly	glycoprotein	848:859	arg1	in vitro processing glycoprotein N-glycans				in vitro processing glycoprotein N-glycans						Toward this goal, we have successfully expressed in E. coli glycoside hydrolases and glycosyltransferases from bacterial and human origins and developed a robust enzymatic platform for in vitro processing glycoprotein N-glycans from high-mannose-type to α2-6- or α2-3-disialylated biantennary complex type.
36985724	4	42	gly	α2-3-disialylated	906:922	arg1	α2-3-disialylated biantennary complex type				α2-3-disialylated biantennary complex type						Toward this goal, we have successfully expressed in E. coli glycoside hydrolases and glycosyltransferases from bacterial and human origins and developed a robust enzymatic platform for in vitro processing glycoprotein N-glycans from high-mannose-type to α2-6- or α2-3-disialylated biantennary complex type.
36135182	3	14	gly	N-glycosylation	374:388	arg2	the N-glycosylation sites			the N-glycosylation sites						sites	By mutating the N-glycosylation sites, we aimed to study the effect of its N-glycan chain on plasminogen activation, fibrin sensitivity, and to observe the physicochemical properties of DSPAα2.
35495330	1	21	gly	glycoproteins	297:309	arg1	various glycoproteins	various glycoproteins				Fterm		glycoproteins			Cyanovirin-N (CV-N) has been shown to reveal broad neutralizing activity against human immunodeficiency virus (HIV) and to specifically bind Manα(1→2)Manα units exposed on various glycoproteins of enveloped viruses, such as influenza hemagglutinin (HA) and Ebola glycoprotein.
35495330	1	89	gly	glycoprotein	380:391	arg1	Ebola glycoprotein	Ebola glycoprotein				Fterm		glycoprotein			Cyanovirin-N (CV-N) has been shown to reveal broad neutralizing activity against human immunodeficiency virus (HIV) and to specifically bind Manα(1→2)Manα units exposed on various glycoproteins of enveloped viruses, such as influenza hemagglutinin (HA) and Ebola glycoprotein.
37382290	2	6	gly	glycopeptide	267:278	arg2	isobaric glycopeptide structures			isobaric glycopeptide structures						glycopeptide	Despite its immense potential, the qualitative and quantitative analysis of isobaric glycopeptide structures remains one of the most daunting hurdles in the field of glycoproteomics.
37040463	4	10	gly	glycoproteins	502:514	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Cell-free protein synthesis systems have matured as a promising approach for making glycoproteins that may overcome current limitations and enable new glycoprotein medicines.
37040463	4	34	gly	glycoprotein	569:580	arg1	new glycoprotein medicines	new glycoprotein medicines				Fterm		glycoprotein			Cell-free protein synthesis systems have matured as a promising approach for making glycoproteins that may overcome current limitations and enable new glycoprotein medicines.
36585837	4	24	gly	glycosylated	439:450	arg1	N -linked glycosylated proteins	N -linked glycosylated proteins				Fterm		proteins			Our objective was to characterize N -linked glycosylated proteins in homogenate swine milk samples collected from sows (n=5/6) during farrowing to represent colostrum, and on day 3 and 14 post-farrowing to represent transitional and mature milk, respectively.
35586945	6	20	gly	N-glycosylation	1094:1108	arg2	specific N-glycosylation sites			specific N-glycosylation sites						sites	Moreover, distinct types of N-glycans on specific N-glycosylation sites regulate DMA-1/LRR-TM receptor function, which, together with three other extracellular proteins, forms the Menorin adhesion complex.
35586945	6	54	gly	N-glycans	1072:1080	arg1	specific N-glycosylation sites			specific N-glycosylation sites	specific N-glycosylation sites		Site			sites	Moreover, distinct types of N-glycans on specific N-glycosylation sites regulate DMA-1/LRR-TM receptor function, which, together with three other extracellular proteins, forms the Menorin adhesion complex.
37026711	6	7	gly	glycosylation	1251:1263	arg2	each glycosylation site			each glycosylation site						site	We modeled four human SLC6 transporters, the serotonin, dopamine, glycine, and B0AT1 transporters, by first simulating all possible combinations of a glycan attached to each glycosylation site followed by investigating the effect of larger, oligo-N-linked glycans to each transporter.
37026711	6	38	gly	attached	1234:1241	arg2	each glycosylation site AND a glycan			each glycosylation site	a glycan					site	We modeled four human SLC6 transporters, the serotonin, dopamine, glycine, and B0AT1 transporters, by first simulating all possible combinations of a glycan attached to each glycosylation site followed by investigating the effect of larger, oligo-N-linked glycans to each transporter.
36089065	2	52	part_of	site	424:427	arg1	a glycoprotein	glycoprotein		site		Fterm	Site	glycoprotein		site	N-glycans are processed in the secretory pathway to form varied ensembles of structures, and diversity at a single site on a glycoprotein is termed 'microheterogeneity'.
37060669	0	48	gly	fucosylated	39:49	arg1	a novel fucosylated trisaccharide				a novel fucosylated trisaccharide						Structural characterization of a novel fucosylated trisaccharide prepared from bacterial exopolysaccharides and evaluation of its prebiotic activity.
35504880	5	47	gly	α-GalNAc-Thr	807:818	arg1	the α-GalNAc-Thr linkage			Thr	the α-GalNAc-Thr linkage					Thr	Interestingly, to achieve glycosylation, C1GalT1 recognizes a high-energy conformation of the α-GalNAc-Thr linkage, negligibly populated in solution.
36192063	7	48	gly	N-glycoproteins	1426:1440	arg1	Eight intact N-glycoproteins	Eight intact N-glycoproteins				Fterm		N-glycoproteins			Eight intact N-glycoproteins responsible for N-glycan biosynthesis were identified as glycosyltransferases.
32691273	11	115	gly	proteins	1628:1635	arg1	elevated O-GlcNAcylation	proteins			elevated O-GlcNAcylation	Fterm		proteins			We found that elevated O-GlcNAcylation of the total proteins displayed a definite correlation in insulin resistance and endoplasmic reticulum stress.
36233110	7	24	gly	N-glycosylation	1553:1567	arg2	multiple N-glycosylation sites			multiple N-glycosylation sites						sites	These results imply that analyses of the glycosylation profiles of fusion proteins containing multiple N-glycosylation sites, such as aflibercept, being done as a part of quality control for the therapeutics manufacturing process or for biosimilar development, can be done with a more applicable outcome by assessing each site separately.
36233110	7	67	gly	glycosylation	1491:1503	arg1	fusion proteins	fusion proteins				Fterm		proteins			These results imply that analyses of the glycosylation profiles of fusion proteins containing multiple N-glycosylation sites, such as aflibercept, being done as a part of quality control for the therapeutics manufacturing process or for biosimilar development, can be done with a more applicable outcome by assessing each site separately.
36430185	1	12	part_of	@	187:187	arg1	A novel and hierarchical hybrid composite	MnO2@CHS		A novel and hierarchical hybrid composite		OGER	Site	MnO2@CHS	Q99698	composite	A novel and hierarchical hybrid composite (MnO2@CHS@SA@Ni) was synthesized utilizing manganese dioxide (MnO2) nanosheets as the core structure, self-assembly chitosan (CHS), sodium alginate (SA) and nickel species (Ni) as surface layers, and it was further incorporated into an epoxy matrix for achieving fire hazard suppression via surface self-assembly technology.
36430185	1	62	part_of	MnO2	183:186	arg1	A novel and hierarchical hybrid composite	MnO2@CHS		A novel and hierarchical hybrid composite		OGER	Site	MnO2@CHS	Q99698	composite	A novel and hierarchical hybrid composite (MnO2@CHS@SA@Ni) was synthesized utilizing manganese dioxide (MnO2) nanosheets as the core structure, self-assembly chitosan (CHS), sodium alginate (SA) and nickel species (Ni) as surface layers, and it was further incorporated into an epoxy matrix for achieving fire hazard suppression via surface self-assembly technology.
36430185	1	66	part_of	CHS	188:190	arg1	A novel and hierarchical hybrid composite	MnO2@CHS		A novel and hierarchical hybrid composite		OGER	Site	MnO2@CHS	Q99698	composite	A novel and hierarchical hybrid composite (MnO2@CHS@SA@Ni) was synthesized utilizing manganese dioxide (MnO2) nanosheets as the core structure, self-assembly chitosan (CHS), sodium alginate (SA) and nickel species (Ni) as surface layers, and it was further incorporated into an epoxy matrix for achieving fire hazard suppression via surface self-assembly technology.
35887202	7	6	gly	O-glycosylated	1094:1107	arg1	the O-glycosylated Thr			the O-glycosylated Thr						Thr	MD simulations of MUC1 glycopeptide showed that the O-glycosylation significantly limits the flexibility of the peptide backbone and side chain of the O-glycosylated Thr.
35887202	7	45	gly	glycopeptide	966:977	arg2	MUC1 glycopeptide			MUC1 glycopeptide						glycopeptide	MD simulations of MUC1 glycopeptide showed that the O-glycosylation significantly limits the flexibility of the peptide backbone and side chain of the O-glycosylated Thr.
32694201	5	3	part_of	has	647:649	arg1	The prefusion trimer AND three receptor-binding domains	The prefusion trimer		three receptor-binding domains		Fterm	Site	trimer		domains	The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide.
35995210	2	11	gly	N-glycosylated	268:281	arg1	TS enzymes	TS enzymes				Fterm		enzymes			TS enzymes are N-glycosylated, but the biological functions of their glycans have remained elusive.
34726173	2	1	gly	glycosylation	296:308	arg1	the long-chain SDR domain			the long-chain SDR domain						domain	The O-linked glycosylation of the long-chain SDR domain mediated by SdgB and SdgA is a key virulence factor that protects the adhesive SDR proteins against host proteolytic attack in order to promote successful tissue colonization, and has also been implicated in staphylococcal agglutination, which leads to sepsis and an immunodominant epitope for a strong antibody response.
36329887	5	30	part_of	FSHα	890:893	arg1	FSHα Asn<sup>52</sup>	FSHα		FSHα Asn<sup>52</sup>		PUBTATOR	AminoAcid	FSHα	100034174	Asn	It was noted that a single biantennary glycan attached to FSHα Asn<sup>52</sup> might preemptively fill the central pocket in this complex and prevent the other two FSH ligands from binding the remaining ligand-binding sites.
37354227	4	58	gly	N-glycosylation	664:678	arg1	recombinant spike proteins	recombinant spike proteins				Fterm		proteins			In this report, we conducted mass spectrometric analysis of the N-glycosylation profiles of recombinant spike proteins derived from four common SARS-CoV-2 variants classified as Variant of Concern, including Alpha, Beta, Gamma, and Delta along with D614G variant spike as a control.
35166741	2	6	gly	glycoproteins	484:496	arg1	homogeneous glycoproteins	homogeneous glycoproteins				Fterm		glycoproteins			Herein, we report optimized methods that can accelerate the semisynthesis of homogeneous glycoproteins based on recombinant expression and chemical conversion.
36266689	0	20	gly	N-glycoproteins	118:132	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			Endoplasmic reticulum-quality control pathway and endoplasmic reticulum-associated degradation mechanism regulate the N-glycoproteins and N-glycan structures in the diatom Phaeodactylum tricornutum.
36838558	3	44	gly	glycans	421:427	arg1	the Gc protein	protein			glycans	Fterm		protein			GcMAF is produced when glycans on the Gc protein are hydrolyzed by α-Sia-ase and β-Gal-ase, leaving an α-GalNAc.
32340215	2	51	gly	glycoprotein	285:296	arg1	a glycoprotein member	a glycoprotein member				Fterm		glycoprotein			MAG is a glycoprotein member of the immunoglobulin superfamily, expressed by myelination cells.
34972858	1	2	gly	glycoproteins	193:205	arg1	biotherapeutic glycoproteins	biotherapeutic glycoproteins				Fterm		glycoproteins			The glycosylation profile of biotherapeutic glycoproteins is a critical quality attribute that is routinely monitored to ensure desired product quality, safety and efficacy.
34972858	1	53	gly	glycosylation	153:165	arg1	biotherapeutic glycoproteins	biotherapeutic glycoproteins				Fterm		glycoproteins			The glycosylation profile of biotherapeutic glycoproteins is a critical quality attribute that is routinely monitored to ensure desired product quality, safety and efficacy.
35887202	8	41	part_of	MUC1	1163:1166	arg1	modeled MY.1E12 Fv and MUC1 O-glycopeptide	MUC1		modeled MY.1E12 Fv and MUC1 O-glycopeptide		PUBTATOR	Site	MUC1	P15941	O-glycopeptide	Docking simulations using modeled MY.1E12 Fv and MUC1 O-glycopeptide, suggest that VH mainly contributes to the recognition of the MUC1 peptide portion while VL mainly binds to the O-glycan part.
32860273	2	24	part_of	IRBP	464:467	arg1	fragments	IRBP		fragments		OGER	Site	IRBP	P10745	fragments	Despite two crystal structures reported on fragments of IRBP and decades of research, the overall structure of IRBP and function within the visual cycle remain unsolved.
37158310	3	77	part_of	contain	452:458	arg1	The catalytic domains AND conserved active-site residues	The catalytic domains		conserved active-site residues						residues	The catalytic domains of GtfB, GtfC and GtfD contain conserved active-site residues for the overall enzymatic activity that relate to hydrolytic glycosidic cleavage of sucrose to glucose and fructose, release of fructose and generation of a glycosyl-enzyme intermediate in the reducing end.
36252443	9	14	gly	glycoproteins	1486:1498	arg1	native glycoproteins	native glycoproteins				Fterm		glycoproteins			The kinetic evaluation showed that EndoBI-1 displayed higher activity on native glycoproteins than PNGase F, with 0.036 mg/mL×min and 0.012 mg/mL×min glycan release, respectively.
35413075	0	23	gly	fucosylated	73:83	arg1	fucosylated structures				fucosylated structures						Increased levels of acidic free-N-glycans, including multi-antennary and fucosylated structures, in the urine of cancer patients.
36754227	2	7	gly	glycosylation	235:247	arg1	position N573			position						position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
36754227	2	9	gly	glycosylation	322:334	arg1	position N559			position						position	BoNT/A1 recognize the luminal domain of SV2A (LD-SV2A) and its glycosylation at position N573 (N573g) or the luminal domain of SV2C (LD-SV2C) and its glycosylation at position N559 (N559g) to bind neural membrane.
34989310	6	39	part_of	residues	1525:1532	arg1	the four enzymes	enzymes		residues		Fterm	AminoAcid	enzymes		residues in	Drug-enzyme active site residue interaction analyses show that the putative inhibitors form non-covalent bonding interactions with key active site residues in each enzyme, suggesting critical target residues in the four enzymes' active sites.
34989310	6	56	part_of	residues	1473:1480	arg1	each enzyme	enzyme		residues		Fterm	AminoAcid	enzyme		residues in	Drug-enzyme active site residue interaction analyses show that the putative inhibitors form non-covalent bonding interactions with key active site residues in each enzyme, suggesting critical target residues in the four enzymes' active sites.
35022400	0	39	gly	glycoproteins	16:28	arg1	Mammalian brain glycoproteins				Mammalian brain glycoproteins						Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues.
36598201	5	42	gly	glycosylation	713:725	arg2	one glycosylation site			one glycosylation site						site	The growth rate of recombinant viruses that lost one glycosylation site was as high as that of the wild-type virus.
36329887	2	38	gly	fully-glycosylated	381:398	arg1	The two most abundant variants	The two most abundant variants				Fterm		variants			The two most abundant variants are fully-glycosylated FSH24 (based on 24 kDa FSHβ band in Western blots) and hypo-glycosylated FSH21 (21 kDa band, lacks βAsn<sup>24</sup> glycans).
36095241	4	55	gly	unglycosylated	824:837	arg1	the unglycosylated peptide			the unglycosylated peptide						peptide	Compared with the unglycosylated peptide, the binding of sT-P320-334 to hACE2 was enhanced to 133% and the inhibition capacity against RBD-hACE2 binding of sTn- and sT-P320-334 was significantly increased up to 150-410%.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	relaxation				relaxation						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	contraction				contraction						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	N-glycosylation				N-glycosylation						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
35211008	9	72	gly	α-1,6-fucosylation	1639:1656	arg1	calcium transient				calcium transient						Detect α-1,6-fucosylation on N-glycosylation, calcium transient, contraction, and relaxation function and related signals.
37319314	1	20	gly	compositions	295:306	arg1	specific sites			specific sites	specific sites		Site			sites	Glycosylation is an essential protein modification occurring on the majority of extracellular human proteins, with mass spectrometry (MS) being an indispensable tool for its analysis, that not only determines glycan compositions, but also the position of the glycan at specific sites via glycoproteomics.
37319314	1	23	gly	sites	357:361	arg1	glycan compositions			sites	glycan compositions					sites	Glycosylation is an essential protein modification occurring on the majority of extracellular human proteins, with mass spectrometry (MS) being an indispensable tool for its analysis, that not only determines glycan compositions, but also the position of the glycan at specific sites via glycoproteomics.
37319314	1	23	gly	sites	357:361	arg1	the glycan			sites	the glycan					sites	Glycosylation is an essential protein modification occurring on the majority of extracellular human proteins, with mass spectrometry (MS) being an indispensable tool for its analysis, that not only determines glycan compositions, but also the position of the glycan at specific sites via glycoproteomics.
36334478	5	1	part_of	amylopectin	1045:1055	arg1	amylopectin composition	amylopectin		amylopectin composition		Fterm	Site	amylopectin		position	FINDINGS We report on how the PVA, amylose, and amylopectin composition affect the properties of the final polymer blend and cryogel formation.
36388954	2	29	part_of	protein	269:275	arg1	five distinct N-glycosylation sites	protein		five distinct N-glycosylation sites		Fterm	Site	protein		sites	We followed the kinetics of intracellular, site-specific glycan processing of a model protein with five distinct N-glycosylation sites and deduced a mathematical model of the secretory pathway that describes a complex set of processing reactions localized in defined intracellular compartments such as the endoplasmic reticulum the Golgi, or the lysosome.
36499311	2	55	gly	O-glycosylation	588:602	arg2	extensive O-glycosylation sites			extensive O-glycosylation sites						sites	We report here its three-dimensional structure, showing the expected bimodular arrangement and also a unique long elongation at its N-terminus containing extensive O-glycosylation sites that form a peculiar arrangement with a protruding loop within the dimer.
35285541	4	5	gly	glycoprotein	617:628	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			Glycosylation influences the expected size of the glycoprotein, which leads to disproportionate MW estimation, in size-dependent methods.
37240090	3	21	gly	glycoprotein	611:622	arg1	a lectin-based glycoprotein microarray	a lectin-based glycoprotein microarray				Fterm		glycoprotein			The analytical methods used were a lectin-based glycoprotein microarray enabling high-throughput glycan analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) as a standard method for the identification of glycan structures.
34695439	2	35	gly	proteins	346:353	arg1	thrombospondin type I repeats	proteins			thrombospondin type I repeats	Fterm		proteins			B3GLCT mediates modification of proteins with thrombospondin type I repeats (TSR) that contain O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs.
34695439	2	46	gly	glycosylation	459:471	arg2	O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs			O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs						motifs	B3GLCT mediates modification of proteins with thrombospondin type I repeats (TSR) that contain O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs.
35247653	0	75	gly	glycoprotein	63:74	arg1	intact glycoprotein	intact glycoprotein				Fterm		glycoprotein			Characterizing a novel hyposialylated erythropoietin by intact glycoprotein and glycan analysis.
34004320	3	49	part_of	receptor	546:553	arg1	The coding region	receptor		The coding region		Fterm	Site	receptor		region	The coding region of the PpelOT/VP-like peptide receptor contained 1497 bp which translationally corresponded to 499 amino acids.
36388954	4	6	gly	protein	904:910	arg1	an increased glycan complexity	protein			an increased glycan complexity	Fterm		protein			Importantly, our model protein can take different routes of the cellular secretory pathway, resulting in an increased glycan complexity of the secreted protein.
35776110	7	19	gly	glycoprotein	1385:1396	arg1	the model glycoprotein fetuin	the model glycoprotein fetuin				Fterm		glycoprotein			We evaluated this RB-GSM approach on N-glycan identification using MS/MS spectra acquired from glycan standards as well as those released from the model glycoprotein fetuin, immunoglobulin G, and human serum samples, which showed the RB-GSM is capable of distinguishing isomeric glycans.
36373229	6	34	gly	O-glycopeptide	1304:1317	arg2	O-glycopeptide identification			O-glycopeptide identification						O-glycopeptide	Using secreted protease of C1 esterase inhibitor (StcE) from enterohemorrhagic Escherichia coli and O-endoprotease OgpA from Akkermansia mucinophila, we explore numerous settings that effect O-glycopeptide identification and show how non-specific and semi-tryptic searches of O-glycopeptide data can produce candidate cleavage motifs.
36373229	6	74	gly	O-glycopeptide	1389:1402	arg2	O-glycopeptide data			O-glycopeptide data						O-glycopeptide	Using secreted protease of C1 esterase inhibitor (StcE) from enterohemorrhagic Escherichia coli and O-endoprotease OgpA from Akkermansia mucinophila, we explore numerous settings that effect O-glycopeptide identification and show how non-specific and semi-tryptic searches of O-glycopeptide data can produce candidate cleavage motifs.
33176830	6	8	gly	N-glycosylation	1134:1148	arg2	non-canonical motifs	Cav3.2		motifs		PUBTATOR		Cav3.2	8912	motifs	Therefore, these newly identified asparagine residues within non-canonical motifs add to those previously reported in canonical sites and suggest that N-glycosylation of Cav3.2 may also occur at non-canonical motifs to control expression of the channel in the plasma membrane.
35219398	1	54	gly	glycosylated	271:282	arg1	several immunoglobulin (Ig)-like ectodomains			several immunoglobulin (Ig)-like ectodomains						ectodomains	Carcinoembryonic cellular adhesion molecules (CEACAMs) serve diverse roles in cell signaling, proliferation, and survival and are made up of one or several immunoglobulin (Ig)-like ectodomains glycosylated in vivo.
32636304	4	22	gly	sites	733:737	arg1	the residues			the residues	the residues		AminoAcid			residues at	In this study, we report one such lectin named horcolin (Hordeum vulgare lectin), seen to lack mitogenicity owing to the divergence in the residues at its carbohydrate-binding sites, which makes it a promising candidate for exploration as an anti-HIV agent.
37146275	1	10	gly	glycosylation	253:265	arg2	glycosylation sites			glycosylation sites						sites	Glycosylation of proteins regulates the life activities of organisms, while abnormalities of glycosylation sites and glycan structures occur in various serious diseases such as cancer.
37146275	1	44	gly	Glycosylation	160:172	arg1	proteins	proteins				Fterm		proteins			Glycosylation of proteins regulates the life activities of organisms, while abnormalities of glycosylation sites and glycan structures occur in various serious diseases such as cancer.
35279850	6	29	gly	fucosylated	1146:1156	arg1	a novel fucosylated N-pentasaccharide				a novel fucosylated N-pentasaccharide						Glycomics profiling in patients with known defects revealed novel features such as the N-tetrasaccharide in ALG2-CDG patients and a novel fucosylated N-pentasaccharide as specific glycomarker for ALG1-CDG.
35925863	11	24	gly	glycoproteins	1761:1773	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			Such serum N-glycomic changes described herein may indicate or correlate to the changes in serum glycoproteins upon COVID-19 infection.
32417172	10	11	gly	Ser121	1375:1380	arg1	The O-GalNAc glycosylation at Ser121			Ser121	The O-GalNAc glycosylation at Ser121					Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
32417172	10	11	gly	Ser121	1375:1380	arg1	The O-GalNAc glycosylation at Ser121			Ser121	The O-GalNAc glycosylation at Ser121					Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
32417172	10	11	gly	Ser121	1375:1380	arg1	The O-GalNAc glycosylation at Ser121			Ser121	The O-GalNAc glycosylation at Ser121					Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
32417172	10	46	gly	glycosylation	1358:1370	arg2	The O-GalNAc glycosylation at Ser121			Ser121						Ser121	The O-GalNAc glycosylation at Ser121 was associated with the stability and activity of p53.
36512245	1	11	part_of	protein	140:146	arg1	the major sites	protein		the major sites		Fterm	Site	protein		sites	The Golgi apparatus is one of the major sites of protein and lipid glycosylation and processing.
35995210	5	23	gly	N-glycosylation	767:781	arg2	up to eight putative N-glycosylation sites			up to eight putative N-glycosylation sites						sites	Our MALDI-TOF mass spectrometry data revealed that up to eight putative N-glycosylation sites were glycosylated.
35995210	5	84	gly	glycosylated	794:805	arg1	up to eight putative N-glycosylation sites			up to eight putative N-glycosylation sites						sites	Our MALDI-TOF mass spectrometry data revealed that up to eight putative N-glycosylation sites were glycosylated.
36031042	2	85	gly	glycoprotein	365:376	arg1	glycoprotein C3	glycoprotein C3				Fterm		glycoprotein			The most abundant complement component, glycoprotein C3, contains two N-glycosylation sites occupied exclusively by this type of glycans.
36031042	2	101	gly	N-glycosylation	395:409	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	The most abundant complement component, glycoprotein C3, contains two N-glycosylation sites occupied exclusively by this type of glycans.
36435009	1	17	gly	core-fucosylated	286:301	arg1	core-fucosylated N-glycans				core-fucosylated N-glycans						A fluorescence-quenching-based assay system to determine the hydrolytic activity of endo-β-N-acetylglucosaminidases (ENGases), which act on the innermost N-acetylglucosamine (GlcNAc) residue of the chitobiose segment of core-fucosylated N-glycans, was constructed using a dual-labeled fluorescent probe with a hexasaccharide structure.
34691043	3	6	gly	glycosylation	427:439	arg2	predicted N-linked glycosylation sites			predicted N-linked glycosylation sites						sites	We previously showed that modulating predicted N-linked glycosylation sites on H3 A/Hong Kong/1/1968 HA identified a conserved epitope at the HA interface.
33085102	6	54	gly	O-glycosylated	907:920	arg1	42 O-glycosylated and 56 N-glycosylated proteins	42 O-glycosylated and 56 N-glycosylated proteins				Fterm		proteins			Analysis using LC/MS-QTOF identified 42 O-glycosylated and 56 N-glycosylated proteins, respectively.
33085102	6	65	gly	N-glycosylated	929:942	arg1	42 O-glycosylated and 56 N-glycosylated proteins	42 O-glycosylated and 56 N-glycosylated proteins				Fterm		proteins			Analysis using LC/MS-QTOF identified 42 O-glycosylated and 56 N-glycosylated proteins, respectively.
36324280	5	47	gly	glycosylation	1093:1105	arg2	the specific glycosylation site			the specific glycosylation site						site	The N-glycome analysis demonstrated that a degree of Hp deglycosylation differs depending on the conditions used for N-glycan release and on the specific glycosylation site, with Asn 241 being the most resistant to deglycosylation under tested conditions.
35887202	8	22	gly	O-glycopeptide	1168:1181	arg2	modeled MY.1E12 Fv and MUC1 O-glycopeptide			modeled MY.1E12 Fv and MUC1 O-glycopeptide						O-glycopeptide	Docking simulations using modeled MY.1E12 Fv and MUC1 O-glycopeptide, suggest that VH mainly contributes to the recognition of the MUC1 peptide portion while VL mainly binds to the O-glycan part.
36797772	2	22	gly	glycoprotein	418:429	arg1	a glycoprotein family	a glycoprotein family				Fterm		glycoprotein			FASCICLIN-LIKE ARABINOGALACTAN PROTEINs (FLAs), which impact plant growth and development, are an excellent example of a glycoprotein family with a complex multidomain structure.
37218360	11	0	part_of	CD16a	1425:1429	arg1	a single CD16a region	CD16a		a single CD16a region		OGER	Site	CD16a	P08637	region	Structural interrogation identified a single CD16a region, proximal to the N162 glycan and the antibody-binding interface, perturbed by the N-glycan composition.
36435935	0	2	gly	oligosaccharyltransferase	74:98	arg1	glycan recognition	oligosaccharyltransferase			glycan recognition	Fterm		oligosaccharyltransferase	281499		Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase.
33006605	8	63	part_of	ACE2	1346:1349	arg1	ACE2 residues	ACE2		ACE2 residues		PUBTATOR	Site	ACE2	Q9BYF1	residues	In addition, we investigated 3705 mutations in SARS-CoV-2 RBD and 11 324 mutations in human ACE2 and found that SARS-CoV-2 neighbor residues G496 and F497 and ACE2 residues D355 and Y41 are critical for the RBD-ACE2 interaction.
33006605	8	68	part_of	SARS-CoV-2	1299:1308	arg1	SARS-CoV-2 neighbor residues G496 and F497	SARS		SARS-CoV-2 neighbor residues G496 and F497		OGER	Site	SARS	P49591	residues	In addition, we investigated 3705 mutations in SARS-CoV-2 RBD and 11 324 mutations in human ACE2 and found that SARS-CoV-2 neighbor residues G496 and F497 and ACE2 residues D355 and Y41 are critical for the RBD-ACE2 interaction.
36631484	9	4	gly	glycoproteins	2351:2363	arg1	model glycoproteins	model glycoproteins				Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
36631484	9	38	gly	glycosylation	2102:2114	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
36631484	9	83	gly	glycans	2334:2340	arg1	model glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
36631484	9	85	gly	glycoproteins	2070:2082	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The growing interest in using in vitro glycoengineering to generate glycoproteins with well-defined glycosylation, provides motivation to demonstrate the capabilities of the GlycoSense method, which we apply here to monitor changes in the protein glycosylation pattern (GlycoPrint) during the in vitro enzymatic modification of the glycans in model glycoproteins.
33486096	3	10	gly	proteins	474:481	arg1	O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
33486096	3	10	gly	proteins	474:481	arg1	The O-linked β-N-acetylglucosamine post-translational modification	proteins			The O-linked β-N-acetylglucosamine post-translational modification	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
33486096	3	10	gly	proteins	474:481	arg1	a glucose-derived metabolic signal	proteins			a glucose-derived metabolic signal	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
33486096	3	40	gly	modification	447:458	arg3	proteins AND The O-linked β-N-acetylglucosamine post-translational modification	proteins			The O-linked β-N-acetylglucosamine post-translational modification	Fterm		proteins			The O-linked β-N-acetylglucosamine post-translational modification (O-GlcNAc) of proteins is a glucose-derived metabolic signal.
34960798	10	32	gly	glycosylation	1731:1743	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	The number of potential N-linked glycosylation sites (PNGS) ranged from 3 to 6 in respective sequences and were located in different positions.
34985300	4	46	gly	glycopeptide	622:633	arg2	both HILIC and FAIMS based glycopeptide enrichment strategies			both HILIC and FAIMS based glycopeptide enrichment strategies						glycopeptide	We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
34985300	4	62	gly	glycosylation	703:715	arg2	257 putative glycosylation sites			257 putative glycosylation sites						sites	We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
34985300	4	81	gly	glycoproteins	730:742	arg1	145 glycoproteins	145 glycoproteins				Fterm		glycoproteins			We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins.
33712585	4	36	part_of	TNT	525:527	arg1	a tuberculosis necrotizing toxin (TNT) domain	TNT		a tuberculosis necrotizing toxin (TNT) domain		OGER	Site	TNT	P13805	domain	The enzymes harbour a tuberculosis necrotizing toxin (TNT) domain and are predominately present in pathogenic species.
35487177	2	3	gly	glycosylation	411:423	arg1	2-azido-6-tert-butyldiphenylsilyl-3,4-di-O-chloroacetyl-2-deoxy-galctopyranosyl imidate and glucose moiety				2-azido-6-tert-butyldiphenylsilyl-3,4-di-O-chloroacetyl-2-deoxy-galctopyranosyl imidate and glucose moiety						The construction of Tn antigen moiety containing α-N-acetylgalactosamine residue was achieved by α-selective glycosylation of 2-azido-6-tert-butyldiphenylsilyl-3,4-di-O-chloroacetyl-2-deoxy-galctopyranosyl imidate and glucose moiety, which acts as a hydrophilic spacer when immobilized on a gold-coated sensor chip.
35311852	8	101	gly	branch-fucosylated	1138:1155	arg1	branch-fucosylated N-glycan isomers				branch-fucosylated N-glycan isomers						To achieve better resolutions on permethylated glycans, different LC conditions were optimized using glycan standards, including core- and branch-fucosylated N-glycan isomers and sialic acid linked isomers, which were both successfully separated.
36740532	9	30	gly	O-glycosylated	1311:1324	arg1	The GPIbα mucin domain			The GPIbα mucin domain						domain	The GPIbα mucin domain is densely O-glycosylated.
36135182	2	34	gly	N-glycosylation	227:241	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo.
36135182	2	28	gly	has	219:221	arg1	N185			N185 and N398						N185 and N398	However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo.
35421698	3	2	gly	N-glycopeptides	511:525	arg2	intact N-glycopeptides			intact N-glycopeptides						N-glycopeptides	Here, we present a comprehensive structure-specific identification of intact N-glycopeptides of Arabidopsis with both HILIC and RAX enrichment, as well as GPSeeker and pGlyco database search.
33343565	1	8	part_of	IgG	196:198	arg1	the IgG Fc domain	IgG		the IgG Fc domain		PUBTATOR	Site	IgG	16059	domain	Therapeutics based on fusing a protein of interest to the IgG Fc domain have been enormously successful, though fewer studies have investigated the vaccine potential of IgG fusions.
33453988	2	39	gly	glycoprotein	415:426	arg1	glycoprotein clustering	glycoprotein clustering				Fterm		glycoprotein			Modification of cell surface receptors with branched N-glycans coordinates cell growth and differentiation by controlling glycoprotein clustering, signaling, and endocytosis.
32709309	8	30	gly	glycoprotein	1569:1580	arg1	several glycoprotein samples	several glycoprotein samples				Fterm		glycoprotein			This method was successfully used to analyze N-linked glycans released from several glycoprotein samples.
35687881	8	49	gly	sialylation	1397:1407	arg1	the N-glycans				the N-glycans						We also found that sialylation of the N-glycans significantly suppressed the immune response.
32417172	2	84	gly	have	318:321	arg1	nuclear or cytoplasmic proteins AND O-GalNAc glycosylation	nuclear or cytoplasmic proteins			O-GalNAc glycosylation	Fterm		proteins			Recently several evidences suggest that nuclear or cytoplasmic proteins might also have O-GalNAc glycosylation.
36959936	10	54	gly	glycoprotein	2028:2039	arg1	native glycoprotein structure	native glycoprotein structure				Fterm		glycoprotein			Nonetheless, animals immunized with the mammalian cell-culture-derived protein were better protected in this challenge model suggesting that more faithfully reproducing the native glycoprotein structure and associated glycosylation of the antigen may be desirable.
37040463	11	20	gly	glycoproteins	1798:1810	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We anticipate that GlycoCAP will facilitate the development and discovery of glycan-based drugs by granting access to a wider variety of possible noncanonical glycan structures and also provide an approach for functionalizing glycoproteins by click chemistry conjugation.
36565355	2	4	part_of	contains	208:215	arg1	The receptor AND an extracellular domain	The receptor		an extracellular domain		Fterm	Site	receptor		domain	The receptor contains an extracellular domain (ECD) with 36 tandem epidermal growth factor-like (EGF) repeats.
35943155	16	106	gly	oligosaccharides	2700:2715	arg1	α-l-fucosyl residues			α-l-fucosyl residues	α-l-fucosyl residues		Site			residues	Microbial α-l-fucosidases are exoglycosidases that remove α-l-fucosyl residues from free oligosaccharides and glycoconjugates and can be also used in transglycosylation reactions to synthesize oligosaccharides.
35364496	5	26	gly	glycosylated	807:818	arg1	Glycinin	Glycinin				Fterm		Glycinin			Glycinin glycosylated with both polysaccharides exhibited enhanced emulsifying activity and stability.
31981002	4	82	part_of	RNase	776:780	arg1	one RNase domain	RNase		one RNase domain		OGER	Site	RNase	P07998	domain	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (74-92%) among various species, retaining one signal peptide, one luminal domain, one serine/threonine kinase domain, one RNase domain, one activation loop, two N-linked glycosylation sites, and several phosphorylation sites.
31981002	4	82	part_of	RNase	776:780	arg1	one signal peptide	RNase		one signal peptide		OGER	Site	RNase	P07998	peptide	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (74-92%) among various species, retaining one signal peptide, one luminal domain, one serine/threonine kinase domain, one RNase domain, one activation loop, two N-linked glycosylation sites, and several phosphorylation sites.
35397991	5	59	gly	N-glycoproteins	889:903	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			N-glycan analysis of N-glycoproteins in the MSG demonstrated that BmGNTI identified and characterized in this study consistently contributed to GlcNAc extension of N-glycans, which led to the accumulation of GlcNAc-extended N-glycans as predominant structures throughout the MSG development.
35380435	0	12	part_of	Receptor-Binding	131:146	arg1	the SARS-CoV-2 Spike Receptor-Binding Domain	Spike Receptor		the SARS-CoV-2 Spike Receptor-Binding Domain		PUBTATOR		Spike Receptor	None		Online Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain.
35380435	0	35	part_of	SARS-CoV-2	114:123	arg1	the SARS-CoV-2 Spike Receptor-Binding Domain	SARS		the SARS-CoV-2 Spike Receptor-Binding Domain		OGER		SARS	P49591		Online Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain.
35380435	0	79	part_of	Spike	125:129	arg1	the SARS-CoV-2 Spike Receptor-Binding Domain	Spike Receptor		the SARS-CoV-2 Spike Receptor-Binding Domain		PUBTATOR		Spike Receptor	None		Online Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain.
36031042	5	69	gly	glycopeptide	841:852	arg2	subsequent LC-MS glycopeptide analysis			subsequent LC-MS glycopeptide analysis						glycopeptide	For this purpose, we developed a novel high-throughput workflow for human C3 concanavalin A lectin affinity enrichment and subsequent LC-MS glycopeptide analysis which enables protein-specific N-glycosylation profiling.
33244686	3	49	gly	glycopeptides	745:757	arg2	glycopeptides			glycopeptides						glycopeptides	Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abundance non-glycosylated peptides, which can be further improved by modifying the mobile phases with ion-pairing agents (IP-HILIC).
33244686	3	68	gly	glycopeptide	626:637	arg2	better glycopeptide identification			better glycopeptide identification						glycopeptide	Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abundance non-glycosylated peptides, which can be further improved by modifying the mobile phases with ion-pairing agents (IP-HILIC).
33244686	3	97	gly	non-glycosylated	779:794	arg1	high-abundance non-glycosylated peptides			high-abundance non-glycosylated peptides						peptides	Hydrophilic interaction chromatography (HILIC) as an orthogonal separation method to reversed-phase liquid chromatography (RPLC) can achieve better glycopeptide identification due to the effective separation between individual glycoforms as well as the separation of glycopeptides from high-abundance non-glycosylated peptides, which can be further improved by modifying the mobile phases with ion-pairing agents (IP-HILIC).
37341462	3	71	gly	O-glycopeptides	580:594	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	However, the pattern of Y ions in O-glycopeptides has not been well studied.
37328468	0	53	gly	glycoproteins	59:71	arg1	divergent Henipavirus fusion glycoproteins	divergent Henipavirus fusion glycoproteins				Fterm		glycoproteins			Structure and antigenicity of divergent Henipavirus fusion glycoproteins.
35405095	3	31	gly	O-glycosylated	486:499	arg1	densely O-glycosylated domains			densely O-glycosylated domains						domains	In particular, the ability to degrade host mucins, a class of proteins carrying densely O-glycosylated domains, provides a competitive advantage in the sustained colonization of niche mucosal environments.
36192063	4	21	gly	N-glycopeptide	803:816	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	With lung cancer as a model system, differential sialylation in cancer tissues relative to adjacent non-tumor tissues was characterized at the intact N-glycopeptide level with N-glycosite information.
36192063	4	68	gly	N-glycosite	829:839	arg2	N-glycosite			N-glycosite						N-glycosite	With lung cancer as a model system, differential sialylation in cancer tissues relative to adjacent non-tumor tissues was characterized at the intact N-glycopeptide level with N-glycosite information.
37037133	4	19	gly	N-glycoproteins	674:688	arg1	1,360, 457, 2,617, and 986 site-specific N-glycans	N-glycoproteins			1,360, 457, 2,617, and 986 site-specific N-glycans	Fterm		N-glycoproteins			We identified 1,360, 457, 2,617, and 986 site-specific N-glycans from 296, 77, 214, and 196 N-glycoproteins in donkey colostrum (DC), donkey mature milk (DM), human colostrum (HC), and human mature milk (HM), respectively.
36797772	3	42	gly	glycans	705:711	arg1	the FAS1 domains			the FAS1 domains	the FAS1 domains		Site			domains	FLAs combine globular fasciclin-like (FAS1) domains, disordered sequences containing glycomotifs for directing addition of O-linked arabinogalactan (AG) glycans, and additional post-translational modifications including N-linked glycans in the FAS1 domains, a cleaved signal peptide at the N-terminus, and often a glycosylphosphatidylinositol (GPI)-anchor signal sequence at the C-terminus.
33085102	5	49	gly	glycosylated	791:802	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			The glycoproteome profiling demonstrates that glycosylated proteins are higher in caprine milk compared to other samples.
33727825	1	16	gly	glycoprotein	159:170	arg1	a highly conserved glycoprotein	a highly conserved glycoprotein				Fterm		glycoprotein			BACKGROUND Ribophorin II (RPN2) is a highly conserved glycoprotein involved in the N-linked glycosylation of multiple proteins.
33727825	1	25	gly	glycosylation	197:209	arg1	multiple proteins	multiple proteins				Fterm		proteins			BACKGROUND Ribophorin II (RPN2) is a highly conserved glycoprotein involved in the N-linked glycosylation of multiple proteins.
36241003	1	34	gly	glycosylated	116:127	arg1	highly glycosylated proteins	highly glycosylated proteins				Fterm		proteins			Mucins are highly glycosylated proteins that make up the mucus covering internal and external surfaces of fish.
36036581	2	42	part_of	protein	329:335	arg1	many important structural motifs	S protein		many important structural motifs		PUBTATOR	Site	S protein	7448	motifs	Previous studies have systematically analyzed site-specific glycan compositions as well as many important structural motifs of the S protein.
35388686	2	38	gly	glycosites	496:505	arg1	glycan compositions			glycosites	glycan compositions					glycosites	Here, we have adapted an HCD-triggered-ultraviolet photodissociation (UVPD) approach for the simultaneous localization of glycosites and full characterization of both glycan compositions and intersaccharide linkages, the latter provided by extensive cross-ring cleavages enabled by UVPD.
35388686	2	38	gly	glycosites	496:505	arg1	intersaccharide linkages			glycosites	intersaccharide linkages					glycosites	Here, we have adapted an HCD-triggered-ultraviolet photodissociation (UVPD) approach for the simultaneous localization of glycosites and full characterization of both glycan compositions and intersaccharide linkages, the latter provided by extensive cross-ring cleavages enabled by UVPD.
35854001	7	10	part_of	lectin	903:908	arg1	the GnT-IVa lectin domain	GnT-IVa lectin		the GnT-IVa lectin domain		PUBTATOR	Site	GnT-IVa lectin	Q9UM21	domain	Comprehensive glycan binding assay using 157 glycans and solution NMR reveal that the GnT-IVa lectin domain selectively interacts with the product N-glycans having a β1-4 GlcNAc branch.
35854001	7	51	part_of	GnT-IVa	895:901	arg1	the GnT-IVa lectin domain	GnT-IVa lectin		the GnT-IVa lectin domain		PUBTATOR	Site	GnT-IVa lectin	Q9UM21	domain	Comprehensive glycan binding assay using 157 glycans and solution NMR reveal that the GnT-IVa lectin domain selectively interacts with the product N-glycans having a β1-4 GlcNAc branch.
37382290	3	44	gly	glycoproteins	534:546	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The ability to distinguish between these complex glycan structures poses a significant challenge, hindering our ability to accurately measure and understand the role of glycoproteins in biological systems.
33176830	0	60	gly	N-glycosylation	53:67	arg2	potential non-canonical N-glycosylation sites			potential non-canonical N-glycosylation sites						sites	Functional identification of potential non-canonical N-glycosylation sites within Cav3.2 T-type calcium channels.
37331167	1	41	gly	glycoproteins	318:330	arg1	insect glycoproteins	glycoproteins			core pentasaccharide	Fterm		glycoproteins			Core α-1,3 mannose is structurally near the core xylose and core fucose on core pentasaccharide from plant and insect glycoproteins.
36252443	7	91	gly	glycoproteins	1171:1183	arg1	bovine milk glycoproteins	bovine milk glycoproteins				Fterm		glycoproteins			infantis ATCC 15697 (B. infantis) was characterized as having the ability to release N-glycans from bovine milk glycoproteins efficiently, without the denaturation.
35091091	6	16	gly	N-glycopeptide	1566:1579	arg2	N-glycopeptide spectra			N-glycopeptide spectra						N-glycopeptide	The new method has been incorporated into the PTM-Shepherd modification analysis tool to work directly with the MSFragger glyco search in the FragPipe graphical user interface, providing a complete computational pipeline for annotation of N-glycopeptide spectra with false discovery rate control of both peptide and glycan components that is both sensitive and robust against false identifications.
35091091	6	25	gly	peptide	1631:1637	arg1	both peptide and glycan components				both peptide and glycan components						The new method has been incorporated into the PTM-Shepherd modification analysis tool to work directly with the MSFragger glyco search in the FragPipe graphical user interface, providing a complete computational pipeline for annotation of N-glycopeptide spectra with false discovery rate control of both peptide and glycan components that is both sensitive and robust against false identifications.
33184803	7	69	gly	glycoprotein	1201:1212	arg1	plasma glycoprotein profile	plasma glycoprotein profile				Fterm		glycoprotein			Mass spectrometry revealed a dramatic alternation in plasma glycoprotein profile in patients with iTTP compared with the healthy controls.
34012659	2	35	gly	N-glycoprotein	310:323	arg1	an N-glycoprotein	an N-glycoprotein				Fterm		N-glycoprotein			Bone marrow stromal cell antigen 2 (BST-2), an N-glycoprotein, has been characterized as an oncogenic factor in several types of cancer.
35343965	7	67	gly	glycoprotein	1345:1356	arg1	the target glycoprotein	the target glycoprotein				Fterm		glycoprotein			The functional importance of glycosylation has been elucidated in several bacteria through mutation of specific genes thought to be involved in the glycosylation process and the study of its impact on the expression of the target glycoprotein and the modifying glycan.
33119615	3	63	gly	glycosylation	556:568	arg1	residue N81			residue N81						residue N81	Our previous in silico study indicated a higher binding affinity for mutated G82S RAGE, which could be caused due to changes in N linked glycosylation at residue N81.
37366623	3	85	gly	glycosylation	752:764	arg2	the glycosylation site			the glycosylation site						site	We also demonstrated that the presence of human γ-globulins in mice and immunity to HSV-1 induced by viral infection in mice significantly reduced replication in their eyes of a mutant virus lacking the glycosylation site but had little effect on the replication of its repaired virus.
35320529	3	74	gly	modified	581:588	arg3	some critical lymphocyte receptors AND glycan structures	some critical lymphocyte receptors			glycan structures	Fterm		receptors			Several galectins have a varying degree of affinity for the N-acetyllactosamine (LacNAc) disaccharide, and some critical lymphocyte receptors can be modified by glycan structures carrying this motif.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	surface lipoproteins	surface lipoproteins				Fterm		lipoproteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	abundant T9SS cargo proteins	proteins				Fterm		proteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	outer membrane β-barrel proteins	proteins				Fterm		proteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
34985300	7	112	gly	O-glycosylation	1178:1192	arg1	outer membrane β-barrel proteins	proteins				Fterm		proteins			Interestingly, a strong bias against the O-glycosylation of abundant proteins exposed to the cell surface such as abundant T9SS cargo proteins, surface lipoproteins, and outer membrane β-barrel proteins was observed.
33085102	8	41	gly	glycoproteins	1124:1136	arg1	Only glycoproteins	Only glycoproteins				Fterm		glycoproteins			Only glycoproteins from human milk have shown positive matching to important human biological pathways, such as vesicle-mediated transport, immune system and hemostasis pathways.
33251387	8	83	part_of	miR-7-5p	869:876	arg1	The binding sites	miR		The binding sites		OGER	Site	miR	Q8BM54	sites	The binding sites of miR-7-5p in the OGT 3' UTR were verified by luciferase reporter assay.
34154738	1	101	gly	fucosylation	105:116	arg1	an Fc N-linked glycan				an Fc N-linked glycan						Core fucosylation of an Fc N-linked glycan affects antibody effector functions, as the absence of fucose increases the antibody dependent cell cytotoxicity (ADCC) response with increased binding to the Fcγ receptors.
37162352	4	11	gly	O-glycosylation	592:606	arg2	325 O-glycosylation sites			325 O-glycosylation sites						sites	In this study, we characterized the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins.
37162352	4	26	gly	glycoproteins	625:637	arg1	226 glycoproteins	226 glycoproteins				Fterm		glycoproteins			In this study, we characterized the O-glycoproteome of F. johnsoniae and identified 325 O-glycosylation sites within 226 glycoproteins.
36189205	2	60	part_of	IgG1	205:208	arg1	the Fc region	IgG1		the Fc region		OGER	Site	IgG1	P01857	region	The conserved N-glycan in the Fc region of IgG1 impacts interaction of IgG with FcγRs and the resulting effector functions, which has led to the design of antibody therapeutics with greatly improved antibody-dependent cell cytotoxicity (ADCC) activities.
36660462	5	10	gly	glycopeptides	868:880	arg2	789 glycopeptides			789 glycopeptides						glycopeptides	A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
36660462	5	13	gly	glycosylated	938:949	arg1	204 glycosylated sites			204 glycosylated sites						sites	A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
36660462	5	31	gly	glycoproteins	964:976	arg1	159 glycoproteins	159 glycoproteins				Fterm		glycoproteins			A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
37162352	6	62	gly	glycoproteins	803:815	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			Bioinformatic localization of the glycoproteins predicted 68 inner membrane proteins, 60 periplasmic proteins, 26 outer membrane proteins, 57 lipoproteins, and 9 proteins secreted by the T9SS.
32357974	3	50	part_of	HLA-bound	545:553	arg1	HLA-bound peptides	HLA		HLA-bound peptides		OGER	Site	HLA		peptides	Here we have analyzed HLA-bound peptides from a variety of allotypes and assessed the distribution of mass spectrometry-detected PTMs, finding deamidation of asparagine or glutamine to be highly prevalent.
36972173	3	50	gly	glycoproteins	558:570	arg1	spike glycoproteins	spike glycoproteins				Fterm		glycoproteins			This necessitates a better understanding of the glycan shields of coronaviruses, which can occlude potential antibody epitopes on spike glycoproteins.
34274480	11	51	part_of	protein	1385:1391	arg1	The deduced amino acid sequence	CBLN3 protein		The deduced amino acid sequence		PUBTATOR	Site	CBLN3 protein	56410	sequence	The deduced amino acid sequence of the porcine CBLN3 protein was 88% identical to mouse CBLN3 and 94% identical to human CBLN3.
36136114	0	66	gly	glycosylated	48:59	arg1	engineered heavily glycosylated fusion proteins	engineered heavily glycosylated fusion proteins				Fterm		proteins			O-Glycoproteomic analysis of engineered heavily glycosylated fusion proteins using nanoHILIC-MS.
36493594	10	26	gly	glycoproteins	1947:1959	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This study is the first comparative analysis for the identification and relative and absolute quantification of N-glycans in glycoproteins with PF-ProA and PA-ProA using UPLC and LC-MS/MS.
36478308	5	22	part_of	peptides	800:807	arg1	isolated glycosylation sites	peptides		isolated glycosylation sites						sites	Three different protease digests were utilised to generate peptides with isolated glycosylation sites.
37186866	5	3	gly	domains	1216:1222	arg1	O-mannosylation			domains	O-mannosylation					domains	Thus, our study identifies the third protein-specific O-mannosylation pathway in mammals and demonstrates that O-mannosylation of IPT domains serves critical functions during epithelial morphogenesis.
37186866	5	31	gly	O-mannosylation	1193:1207	arg1	IPT domains			IPT domains						domains	Thus, our study identifies the third protein-specific O-mannosylation pathway in mammals and demonstrates that O-mannosylation of IPT domains serves critical functions during epithelial morphogenesis.
36435009	2	11	gly	fucosylated	539:549	arg1	core fucosylated N-glycans				core fucosylated N-glycans						The fluorogenic probe was evaluated using a variety of ENGases, including Endo-M W251N mutant, Endo-F3, and Endo-S, which recognize core fucosylated N-glycans.
34726173	1	54	gly	serine-aspartate	230:245	arg1	SDR			serine	SDR					serine	The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA.
34726173	1	54	gly	serine-aspartate	230:245	arg1	serine-aspartate repeat			serine	serine-aspartate repeat					serine	The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA.
34726173	1	80	gly	containing	219:228	arg1	staphylococcal adhesive proteins AND serine-aspartate repeat (SDR) domains	staphylococcal adhesive proteins			serine-aspartate repeat (SDR) domains	Fterm		proteins			The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA.
35921896	6	6	gly	asparagine-linked	934:950	arg1	the CD16a asparagine-linked (N)-glycans			asparagine	the CD16a asparagine-linked (N)-glycans					asparagine	Another relevant variable shown to affect affinity is composition of the CD16a asparagine-linked (N)-glycans.
36135182	2	48	part_of	has	219:221	arg1	wild-type DSPAα2 AND two N-glycosylation sites	wild-type DSPAα2		two N-glycosylation sites		PUBTATOR	Site	DSPAα2	112321404	sites	However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo.
36670130	7	19	gly	N-glycosylation	1162:1176	arg2	all N-glycosylation sites			all N-glycosylation sites						sites	Intriguingly, the removal of all N-glycosylation sites on MP84 increased adhesion to host cells and enhanced the induction of cytokine secretion from BMDCs compared with that on MP84 carrying WT N-glycans.
36812846	5	46	gly	α-mannosylation	767:781	arg1	the C-3 and C-6 positions			the C-3 and C-6 positions						positions	Sequential regioselective α-mannosylation at the C-3 and C-6 positions of 2,3,4,6-unprotected galactose residues in galactosylchitobiose derivatives was demonstrated.
36812846	5	61	gly	positions	802:810	arg1	Sequential regioselective α-mannosylation			positions	Sequential regioselective α-mannosylation					positions	Sequential regioselective α-mannosylation at the C-3 and C-6 positions of 2,3,4,6-unprotected galactose residues in galactosylchitobiose derivatives was demonstrated.
36838558	0	32	gly	Protein	107:113	arg1	the Glycan Structure	Protein			the Glycan Structure	Fterm		Protein			Investigation of the Protective Effect for GcMAF by a Glycosidase Inhibitor and the Glycan Structure of Gc Protein.
35495330	5	1	gly	residues	1250:1257	arg1	glutamic acid			glutamic acid						acid	We were utilizing a triazole bioisostere linkage to form the respective mannosylated-derivative on the HA peptide sequence of residues glutamine, glycine, and glutamic acid.
36925243	0	2	gly	β-cyclodextrin	81:94	arg1	isomalto/malto-polysaccharides	β-cyclodextrin			isomalto/malto-polysaccharides	Fterm		β-cyclodextrin			Cost-effective and controllable synthesis of isomalto/malto-polysaccharides from β-cyclodextrin by combined action of cyclodextrinase and 4,6-α-glucanotransferase GtfB.
36380917	5	45	gly	peptides	1255:1262	arg1	the therapeutic properties				the therapeutic properties						Our results indicated that the selection of an appropriate position (to avoid hindering the peptide-receptor binding) is crucial for N-glycan modification and its sialylation to improve the therapeutic properties of the modified peptides.
36990248	1	28	gly	O-glycosylation	123:137	arg1	secreted and membrane-bound proteins	secreted and membrane-bound proteins				Fterm		proteins			O-glycosylation of secreted and membrane-bound proteins is an important post-translational modification that affects recognition of cell surface receptors, protein folding, and stability.
37219590	4	7	gly	glycoprotein	750:761	arg1	glycoprotein models	glycoprotein models				Fterm		glycoprotein			Here, a broadening of the scope of the software to analyse and validate the overall conformation of N-glycans is presented, focusing on a newly compiled set of glycosidic linkage torsional preferences harvested from a curated set of glycoprotein models.
34102146	4	31	gly	glycopeptide	892:903	arg2	intact glycopeptide identification			intact glycopeptide identification						glycopeptide	Results identified haptoglobin as the protein associated with HexNAc4-Hex5-NeuAc2, thus directly linking glycan imaging with intact glycopeptide identification.
36892535	4	24	gly	O-glycosylated	598:611	arg1	homogeneously O-glycosylated MUC1 peptides			homogeneously O-glycosylated MUC1 peptides						peptides	To better understand the role of MUC1 glycosylation in interaction and adhesion of cancer cells, we prepared a panel of homogeneously O-glycosylated MUC1 peptides using a quantitative chemoenzymatic approach.
34274643	1	62	gly	glycoprotein	342:353	arg1	an intact glycoprotein	an intact glycoprotein				Fterm		glycoprotein			Separations based on combinations of 2.1 mm I.D. high-performance affinity microcolumns and capillary electrophoresis were developed and used to characterize the glycoforms of an intact glycoprotein.
34274643	1	79	gly	glycoforms	318:327	arg1	an intact glycoprotein	an intact glycoprotein				Fterm		glycoprotein			Separations based on combinations of 2.1 mm I.D. high-performance affinity microcolumns and capillary electrophoresis were developed and used to characterize the glycoforms of an intact glycoprotein.
35123071	6	2	gly	glycoproteins	1039:1051	arg1	41 glycoproteins	41 glycoproteins				Fterm		glycoproteins			A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35123071	6	16	gly	glycoproteins	1133:1145	arg1	these glycoproteins	these glycoproteins				Fterm		glycoproteins			A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35123071	6	31	gly	glycopeptides	1061:1073	arg2	43 glycopeptides			43 glycopeptides						glycopeptides	A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35941485	1	15	gly	glycosylated	175:186	arg1	Proteoglycans	proteins			Proteoglycans	Fterm		proteins			Proteoglycans are heavily glycosylated proteins, covalently linked to one or more glycosaminoglycan (GAG) chains, abundantly expressed in the extracellular matrix (ECM).
36055406	0	65	gly	glycosylation	21:33	arg1	GPI-anchored proteins	GPI-anchored proteins				Fterm		proteins			ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.
33006605	2	64	part_of	protein	310:316	arg1	The receptor-binding domain	S protein		The receptor-binding domain		PUBTATOR	Site	S protein	Q15517	domain	The receptor-binding domain (RBD) of SARS-CoV-2 S protein directly interacts with the human angiotensin-converting enzyme 2 (ACE2) on the host cell membrane.
37398203	5	99	part_of	Fd	749:750	arg1	the Fc/2 and Fd fragments	Fd		the Fc/2 and Fd fragments		Cterm	Site	Fd		fragments	MS analysis of large antibody fragments (the light chain, as well as the Fc/2 and Fd fragments of the heavy chain) confirms the monoclonal nature of this component of the anti-PF4 antibodies repertoire, and reveals the presence of a fully mature complex biantennary N-glycan within its Fd segment.
36740532	0	83	gly	glycoprotein	35:46	arg1	platelet glycoprotein	platelet glycoprotein				Fterm		glycoprotein			Comprehensive analysis of platelet glycoprotein Ibα ectodomain glycosylation.
35395480	5	13	gly	pectin	602:607	arg1	monosaccharide compositions	pectin			monosaccharide compositions	Fterm		pectin			Structural characteristics of pectin, including the molecular weight, degree of methoxylation and acetylation, and monosaccharide compositions were measured.
36870092	9	54	gly	N-glycopeptides	1299:1313	arg2	1717 quantified intact N-glycopeptides			1717 quantified intact N-glycopeptides						N-glycopeptides	Among 1717 quantified intact N-glycopeptides, 104 DEGPs were determined (fold change ≥ 1.5 and p value < 0.05).
36370046	7	1	gly	glycosylation	994:1006	arg1	the spike protein receptor-binding domain			the spike protein receptor-binding domain						domain	Herein, we used designed combinations of glycoengineering enzymes to simplify and control the glycosylation profile of the spike protein receptor-binding domain (RBD).
34960798	9	62	part_of	glycoprotein	1601:1612	arg1	aa sequences	glycoprotein		aa sequences		Fterm	Site	glycoprotein		sequences	In contrast, aa sequences of surface glycoprotein exhibited higher variability confirming type-specific variation in the SU5 epitope.
36447399	5	65	part_of	Fab	1222:1224	arg1	the Fab constant region	Fab		the Fab constant region		PUBTATOR	Site	Fab	2187	region	Since only about 20% of human IgG1 have a Fab glycan, we extended the application of this approach by using molecular modeling to introduce N-glycosylation sites in the Fab constant region of other therapeutic monoclonal antibodies.
35293727	3	46	gly	glycosylation	478:490	arg2	multiple glycosylation sites			multiple glycosylation sites						sites	O-Antigen polysaccharides are typically polydisperse, and carrier proteins can have multiple glycosylation sites.
35780667	6	11	gly	composition	916:926	arg1	neutral sugars			position	neutral sugars					position	The different structure of the two polysaccharide fractions was also confirmed by the composition in neutral sugars and the degrees of methylation and acetylation.
36244450	5	52	gly	glycosylation	823:835	arg1	receptor glycan-binding properties				receptor glycan-binding properties						The construct was expressed in different glycosylation-mutant cell lines to study the influence of differential glycosylation on receptor glycan-binding properties.
37186866	3	58	part_of	IPT	864:866	arg1	IPT domains	IPT		IPT domains		OGER	Site	IPT	Q9H3H1	domains	Here, we report that the TMEM260 gene encodes an ER-located protein O-mannosyltransferase that selectively glycosylates IPT domains.
35941485	7	0	part_of	CS/DS	2002:2006	arg1	oversulfated CS/DS domains	CS		oversulfated CS/DS domains		PUBTATOR	Site	CS	1431	domains	The latter method entails the in-laboratory manufacturing of a simple yet sturdy interface for the online CZE coupling to ESI-MS and the optimization of the coupled system for total analysis of regularly sulfated and irregularly, i.e., under- and oversulfated CS/DS domains.
36192063	2	36	gly	N-glycopeptides	447:461	arg2	intact N-glycopeptides level			intact N-glycopeptides level						N-glycopeptides	Mass spectrometry-based quantitative N-glycoproteomics has been widely adopted for quantitative analysis of aberrant sialylation, yet multiplexing method at intact N-glycopeptides level is still lacking.
32409323	1	49	gly	glycoproteins	214:226	arg1	high mannose N-linked glycoproteins				high mannose N-linked glycoproteins						OBJECTIVES FBXO6, a component of the ubiquitin E3 ligases, has been shown to bind high mannose N-linked glycoproteins and act as ubiquitin ligase subunits.
35285541	3	43	gly	heterogeneity	522:534	arg1	the attached carbohydrates				the attached carbohydrates						Determination of MW of heavily glycosylated proteins, such as Fc-fusion proteins, is seriously hampered by the physicochemical characteristics and heterogeneity of the attached carbohydrates.
35285541	3	37	gly	glycosylated	406:417	arg1	heavily glycosylated proteins	proteins				Fterm		proteins			Determination of MW of heavily glycosylated proteins, such as Fc-fusion proteins, is seriously hampered by the physicochemical characteristics and heterogeneity of the attached carbohydrates.
36089065	4	0	gly	glycoproteins	749:761	arg1	reporter N-linked glycoproteins	reporter N-linked glycoproteins				Fterm		glycoproteins			We tested this hypothesis via expression of reporter N-linked glycoproteins in N-acetylglucosaminyltransferase MGAT1-null HEK293 cells to produce immature Man5GlcNAc2 glycoforms (38 glycan sites total).
36089065	4	57	gly	glycoforms	854:863	arg1	immature Man5GlcNAc2 glycoforms				immature Man5GlcNAc2 glycoforms						We tested this hypothesis via expression of reporter N-linked glycoproteins in N-acetylglucosaminyltransferase MGAT1-null HEK293 cells to produce immature Man5GlcNAc2 glycoforms (38 glycan sites total).
35481895	0	13	gly	protein	94:100	arg1	N-	protein			N-	Fterm		protein			Characterizing the N- and O-linked glycans of the PGF-CTERM sorting domain-containing S-layer protein of Methanoculleus marisnigri.
35481895	0	13	gly	protein	94:100	arg1	O-linked glycans	protein			O-linked glycans	Fterm		protein			Characterizing the N- and O-linked glycans of the PGF-CTERM sorting domain-containing S-layer protein of Methanoculleus marisnigri.
36181511	0	61	gly	glycopeptide	35:46	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	Evaluation of absorbent cotton for glycopeptide enrichment.
36746580	4	17	gly	N-glycans	845:853	arg1	25 N-glycoproteins	N-glycoproteins			N-glycans	Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
36746580	4	37	gly	N-glycans	766:774	arg1	10 N-glycoproteins	N-glycoproteins			N-glycans	Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
36746580	4	68	gly	N-glycoproteins	782:796	arg1	10 N-glycoproteins	10 N-glycoproteins				Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
36746580	4	82	gly	N-glycoproteins	861:875	arg1	25 N-glycoproteins	25 N-glycoproteins				Fterm		N-glycoproteins			With milk maturation, 33 site-specific N-glycans on 10 N-glycoproteins increased significantly, and 113 site-specific N-glycans on 25 N-glycoproteins decreased significantly.
32691273	14	87	gly	proteins	2314:2321	arg1	O-GlcNAc levels	proteins			O-GlcNAc levels	Fterm		proteins			In conclusion, our results suggest that OGT regulates insulin receptor signaling and unfolded protein response by modulating O-GlcNAc levels of total proteins, in response to insulin resistance.
37348475	0	8	gly	glycosylation	90:102	arg1	a classical arabinogalactan protein precursor	a classical arabinogalactan protein precursor				Fterm		precursor			Glycosylphosphatidylinositol-anchoring is required for the proper transport and extensive glycosylation of a classical arabinogalactan protein precursor in tobacco BY-2 cells.
36870654	0	69	gly	glycosylation	36:48	arg1	carbohydrate substrate binding				carbohydrate substrate binding						Insights into the effect of protein glycosylation on carbohydrate substrate binding.
35647713	4	41	gly	glycoproteins	770:782	arg1	viral glycoproteins	glycoproteins			N-linked glycans	Fterm		glycoproteins			We describe herein a one-step enzymatic conjugation approach for the covalent attachment of small molecule adjuvants to N-linked glycans of viral glycoproteins.
36972173	7	19	gly	glycosylation	880:892	arg2	glycosylation sites			glycosylation sites						sites	Conversely, glycosylation sites in the S2 domain are highly conserved and contain a low abundance of oligomannose-type glycans, suggesting a low glycan shield density.
37162352	12	64	gly	glycosylated	1526:1537	arg1	The glycosylated domains			The glycosylated domains						domains	The glycosylated domains were mainly localized to the periplasm.
34274643	0	75	gly	glycoprotein	111:122	arg1	alpha1-acid glycoprotein	alpha1-acid glycoprotein				Fterm		glycoprotein			Glycoprotein analysis using lectin microcolumns and capillary electrophoresis: Characterization of alpha1-acid glycoprotein by combined separation methods.
36754227	3	16	part_of	LD-SV2C	675:681	arg1	residues	SV2C		residues		OGER	Site	SV2C	Q496J9	residues	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
36754227	3	45	part_of	SV2C	484:487	arg1	the luminal domain	SV2C		the luminal domain		OGER	Site	SV2C	Q496J9	domain	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
35104505	6	54	gly	glycoprotein	1212:1223	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			To clarify this mechanism, we measured the in vitro activity of purified GnT-VΔN toward various kinds of substrates (oligosaccharide, glycohexapeptide, and glycoprotein) using HPLC and a UDP-Glo assay.
35943155	12	10	gly	fucosylated	1996:2006	arg1	fucosylated glycans				fucosylated glycans						These results give insight into the great diversity of α-l-fucosidases from the infant gut microbiota, thus supporting the hypothesis that fucosylated glycans are crucial for shaping the newborn microbiota composition.
36892535	7	30	gly	glycosylated	1067:1078	arg1	the glycosylated MUC1 peptides			the glycosylated MUC1 peptides						peptides	To decipher the molecular mechanism for the observed adhesion we investigated conformation of the glycosylated MUC1 peptides by NMR.
36580234	8	23	gly	glycoproteins	1324:1336	arg1	target glycoproteins	target glycoproteins				Fterm		glycoproteins			The precision structural interpretation of site-specific glycans and subsequent intervention of target glycoproteins and related glycosyltransferases are of great value for the development of new diagnostic and therapeutic approaches for different diseases.
35887202	9	2	gly	anti-glycopeptide	1430:1446	arg2	anti-glycopeptide			anti-glycopeptide						anti-glycopeptide	The VH/VL-shared recognition mode of this antibody may be used as a template for the rational design and development of anti-glycopeptide antibodies.
35104505	3	14	gly	glycoprotein	567:578	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			However, it is still unclear how GnT-V selectively acts on glycoprotein substrates.
37093892	12	37	gly	glycosylation	1747:1759	arg2	the highly conserved glycosylation site N8			the highly conserved glycosylation site N8						site	We identified three major epitopic regions: the loops at the apex of the RBD, which likely mediate interactions between Env protomers to form Env trimers, a loop located in the vicinity of the heparan binding site, and a region proximal to the highly conserved glycosylation site N8.
34102146	5	49	part_of	proteins	1042:1049	arg1	S- glycopeptides	proteins		S- glycopeptides		Fterm	Site	proteins		glycopeptides	In total, our spatially resolved glycoproteomics technique identified over 400 N-, O-, and S- glycopeptides from over 30 proteins, demonstrating the diverse array of glycosylation present on the tissue slices and the sensitivity of our technique.
36189205	3	22	gly	N-glycosylation	450:464	arg1	receptor interactions	receptor interactions				Fterm		receptor			Studies have suggested that also N-glycosylation of the FcγRIII affects receptor interactions with IgG, but detailed studies of the interaction of IgG1 and FcγRIIIa with distinct N-glycans have been hindered by the natural heterogeneity in N-glycosylation.
36385669	5	1	gly	glycosylation	874:886	arg2	a glycosylation site			a glycosylation site						site	Furthermore, based on sequence Ans-X-Ser/Thr, where X is any amino acid except proline, a glycosylation site was determined on asparagine 36.
37060669	8	44	gly	fucosylated	1185:1195	arg1	fucosylated human milk oligosaccharides				fucosylated human milk oligosaccharides						This work describes a novel fucosylated oligosaccharide and its preparation method, and the obtained trisaccharide may serve as a promising candidate for fucosylated human milk oligosaccharides.
37060669	8	45	gly	fucosylated	1059:1069	arg1	a novel fucosylated oligosaccharide				a novel fucosylated oligosaccharide						This work describes a novel fucosylated oligosaccharide and its preparation method, and the obtained trisaccharide may serve as a promising candidate for fucosylated human milk oligosaccharides.
36631484	6	19	gly	glycoprotein	1237:1248	arg1	glycoprotein engineering	glycoprotein engineering				Fterm		glycoprotein			Here we report a simplified approach to assist in monitoring glycosylation features during glycoprotein engineering, that employs flow cytometry using fluorescent microspheres chemically coupled to high-specificity glycan binding reagents.
35411940	2	50	gly	proteins	329:336	arg1	N-linked high-mannose glycans	proteins			N-linked high-mannose glycans	Fterm		proteins			Sickle cell disease and infection by Plasmodium falciparum cause oxidative stress that induces aggregates of cross-linked proteins with N-linked high-mannose glycans (HMGs).
35091091	4	74	gly	glycopeptides	784:796	arg1	the glycan composition			glycopeptides	the glycan composition					glycopeptides	Here, we have developed a new method for determining the glycan composition of N-linked glycopeptides fragmented by collisional or hybrid activation that uses multiple sources of information from the spectrum, including observed glycan B-type (oxonium) and Y-type ions and mass and precursor monoisotopic selection errors to discriminate between possible glycan candidates.
35349291	5	38	gly	N-glycopeptide	622:635	arg2	Our recently developed intact N-glycopeptide search engine GPSeeker			Our recently developed intact N-glycopeptide search engine GPSeeker						N-glycopeptide	Our recently developed intact N-glycopeptide search engine GPSeeker has made structure-specific characterization of N-glycosylation possible with structure-diagnostic fragment ions from selective fragmentation of N-glycan moieties.
37294165	2	11	part_of	has	354:356	arg1	SPINK13 AND Pro-Asn-Val-Thr	SPINK13		Pro-Asn-Val-Thr		OGER	Site	SPINK13	Q1W4C9	Pro-Asn-Val-Thr	Although SPINK13 has a consensus sequence (Pro-Asn-Val-Thr) for N-glycosylation, the existence of N-glycosylation and its functions are still unclear.
37294165	2	11	part_of	has	354:356	arg1	SPINK13 AND a consensus sequence	SPINK13		a consensus sequence		OGER	Site	SPINK13	Q1W4C9	sequence	Although SPINK13 has a consensus sequence (Pro-Asn-Val-Thr) for N-glycosylation, the existence of N-glycosylation and its functions are still unclear.
33542268	6	19	gly	glycosylation	1388:1400	arg2	the sole stabilizing N-linked glycosylation site			the sole stabilizing N-linked glycosylation site						site	To uncover potential inherent F3 instability not easily observed under typical culture conditions, we genetically eliminated the sole stabilizing N-linked glycosylation site (N249) from select clinically-identified F3 mutants.
33542268	6	19	gly	glycosylation	1388:1400	arg2	N249			N249						N249	To uncover potential inherent F3 instability not easily observed under typical culture conditions, we genetically eliminated the sole stabilizing N-linked glycosylation site (N249) from select clinically-identified F3 mutants.
35304921	11	92	gly	glycoproteins	1415:1427	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A striking example concerns the involvement of glycan-binding galectins in the regulation of the molecular homeostasis of glycoproteins at the cell surface through the formation of lattices or endocytic pits according to the glycolipid-lectin (GL-Lect) hypothesis.
35281276	3	36	gly	glycosylated	289:300	arg1	Many cellular receptors	Many cellular receptors				Fterm		receptors			Many cellular receptors are glycosylated, and their activity can be modulated through changes in glycan structure.
33006605	7	31	gly	glycosylation	1122:1134	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Moreover, many mutations in N-linked glycosylation sites can increase the stability of the S protein.
35017635	5	19	gly	fucosylated	917:927	arg1	these fucosylated glycans				these fucosylated glycans						Moreover, these fucosylated glycans can serve as ligands for DC-SIGN positive tumour-associated macrophages, modulating their activation and inducing the production of IL-10.
37037133	6	24	part_of	N-glycoproteins	1009:1023	arg1	the molecular composition	MFGM N-glycoproteins		the molecular composition		OGER	Site	MFGM N-glycoproteins	Q08431	position	The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
37037133	6	38	part_of	N-glycoproteins	1211:1225	arg1	the composition	MFGM N-glycoproteins		the composition		OGER	Site	MFGM N-glycoproteins	Q08431	position	The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
36529080	9	65	gly	present	1322:1328	arg1	a new N-linked glycosylation site			a new N-linked glycosylation site						site	we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36529080	9	64	gly	glycosylation	1300:1312	arg1	some variants	variants		site		Fterm		variants		site	we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36775128	3	73	gly	possessing	444:453	arg1	a conserved adhesin AND a thrombospondin repeat (TSR) domain	a conserved adhesin			a thrombospondin repeat (TSR) domain	Fterm		adhesin			At present, the only approved vaccine against any apicomplexan parasite targets a conserved adhesin possessing a thrombospondin repeat (TSR) domain.
33952641	2	56	gly	glycoproteins	340:352	arg1	host-encoded, surface-expressed glycoproteins	host-encoded, surface-expressed glycoproteins				Fterm		glycoproteins			One common host-evasion strategy is the downregulation of host-encoded, surface-expressed glycoproteins.
36585837	9	12	gly	glycoproteins	1324:1336	arg1	220 glycoproteins	220 glycoproteins				Fterm		glycoproteins			Shotgun proteome analysis identified 545 N-linked and glutamine, Q, -linked, glycosylation (P>0.75 for deamidation) sites on 220 glycoproteins in sow milk.
36089065	9	41	gly	glycosylation	1771:1783	arg2	each glycosylation site			each glycosylation site						site	These data suggest higher order local structures surrounding each glycosylation site contribute to the efficiency of modification both in vitro and in vivo to establish the spectrum of microheterogeneity in N-linked glycoproteins.
36089065	9	50	gly	glycoproteins	1921:1933	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			These data suggest higher order local structures surrounding each glycosylation site contribute to the efficiency of modification both in vitro and in vivo to establish the spectrum of microheterogeneity in N-linked glycoproteins.
35183770	2	26	gly	glycoproteins	514:526	arg1	distinctive glycoproteins	distinctive glycoproteins				Fterm		glycoproteins			In this study, by mapping the most comprehensive N-glycoproteome of human spermatozoa using our recently developed site-specific glycoproteomic approaches, we show that spermatozoa contain a number of distinctive glycoproteins, which are mainly involved in spermatogenesis, acrosome reaction and sperm:oocyte membrane binding, and fertilization.
36746580	1	58	gly	N-glycosylated	198:211	arg1	Human milk fat globule membrane (MFGM) proteins	Human milk fat globule membrane (MFGM) proteins				Fterm		proteins			Human milk fat globule membrane (MFGM) proteins, which are N-glycosylated, play essential roles in neonatal development and physiological health.
37093892	10	94	gly	glycosylation	1308:1320	arg2	the glycosylation sites			the glycosylation sites						sites	We defined the regions targeted by the nAbs using mutant SU proteins modified at the glycosylation sites, RBD functional subregions, and genotype-specific sequences that present properties of B-cell epitopes.
36571497	5	11	part_of	AOS	1029:1031	arg1	The different AOS binding sites	AOS		The different AOS binding sites		OGER	Site	AOS		sites	The different AOS binding sites on β-LgA were identified by NMR chemical shift perturbation analyses and showed diverse compositions of charged, polar and hydrophobic residues.
32790871	5	5	part_of	epitope	760:766	arg1	N-CSP	CSP		epitope		OGER	Site	CSP	Q9H3Z4	epitope	The crystal structure at 1.85-Å resolution revealed that 5D5 binds an α-helical epitope in N-CSP with high affinity through extensive shape and charge complementarity and the unusual utilization of an antibody N-linked glycan.
31941975	9	55	gly	N-glycopeptides	1636:1650	arg2	tri- and tetra-antennary N-glycopeptides			tri- and tetra-antennary N-glycopeptides						N-glycopeptides	Specifically, outer fucosylation was dominant in tri- and tetra-antennary N-glycopeptides, while core fucosylation was dominant in the mono-, bi-antennary and hybrid types of N-glycoproteins in human plasma.
31941975	9	66	gly	N-glycoproteins	1737:1751	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			Specifically, outer fucosylation was dominant in tri- and tetra-antennary N-glycopeptides, while core fucosylation was dominant in the mono-, bi-antennary and hybrid types of N-glycoproteins in human plasma.
33268543	7	62	gly	glycosylation	1054:1066	arg1	N102			N102						N102	This mutation, however, impaired the maturation of NLGN4 protein by inhibiting N-linked glycosylation at an adjacent residue (N102), which is conserved in all NLGNs.
33268543	7	62	gly	glycosylation	1054:1066	arg1	an adjacent residue			an adjacent residue						residue	This mutation, however, impaired the maturation of NLGN4 protein by inhibiting N-linked glycosylation at an adjacent residue (N102), which is conserved in all NLGNs.
31981002	4	6	gly	glycosylation	824:836	arg2	one signal peptide			one signal peptide						peptide	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (74-92%) among various species, retaining one signal peptide, one luminal domain, one serine/threonine kinase domain, one RNase domain, one activation loop, two N-linked glycosylation sites, and several phosphorylation sites.
31981002	4	6	gly	glycosylation	824:836	arg2	two N-linked glycosylation sites			two N-linked glycosylation sites						sites	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (74-92%) among various species, retaining one signal peptide, one luminal domain, one serine/threonine kinase domain, one RNase domain, one activation loop, two N-linked glycosylation sites, and several phosphorylation sites.
35183770	3	11	gly	extracellular	716:728	arg1	regions			regions						regions	Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
35183770	3	15	gly	observed	669:676	arg2	14 glycoproteins AND Heavy fucosylation	14 glycoproteins			Heavy fucosylation	Fterm		glycoproteins			Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
35183770	3	16	gly	glycoproteins	684:696	arg1	14 glycoproteins	14 glycoproteins				Fterm		glycoproteins			Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
35183770	3	47	gly	fucosylation	653:664	arg1	14 glycoproteins	14 glycoproteins				Fterm		glycoproteins			Heavy fucosylation is observed on 14 glycoproteins mostly located at extracellular and cell surface regions in spermatozoa but not in other tissues.
36037649	3	40	gly	glycoprotein	705:716	arg1	the S-layer glycoprotein	the S-layer glycoprotein				Fterm		glycoprotein			In the present report, nuclear magnetic resonance spectroscopy was used to define a tetrasaccharide N-linked to both archaellins, building blocks of the archaeal swimming device (the archaellum), and the S-layer glycoprotein that comprises the protein shell surrounding the Hbt.
32130226	1	29	gly	glycosylation	244:256	arg1	substrate proteins	substrate proteins				Fterm		proteins			The oligosaccharyl transferase (OST) protein complex mediates the N-linked glycosylation of substrate proteins in the endoplasmic reticulum (ER), which regulates stability, activity, and localization of its substrates.
32943699	2	56	gly	glycosylation	458:470	arg1	the Fc region			the Fc region						region	The inflammatory capability of both maternal and neonatal antibodies is governed by N-linked glycosylation of the Fc region, and though this has been examined extensively in adults, there is currently little information regarding antibody glycosylation patterns during early postnatal life.
35995381	1	70	part_of	has	158:160	arg1	Tg AND many glycosylation sites	Tg		many glycosylation sites		PUBTATOR	Site	Tg	7038	sites	Human thyroglobulin (Tg), which has many glycosylation sites, is an essential protein produced by the human thyroid glands.
35995381	1	70	part_of	has	158:160	arg1	Human thyroglobulin AND many glycosylation sites	Human thyroglobulin		many glycosylation sites		PUBTATOR	Site	Human thyroglobulin	7038	sites	Human thyroglobulin (Tg), which has many glycosylation sites, is an essential protein produced by the human thyroid glands.
35995381	1	70	part_of	has	158:160	arg1	an essential protein AND many glycosylation sites	an essential protein		many glycosylation sites		Fterm	Site	protein		sites	Human thyroglobulin (Tg), which has many glycosylation sites, is an essential protein produced by the human thyroid glands.
32356523	0	30	gly	glycoprotein	117:128	arg1	glycoprotein transport	glycoprotein transport				Fterm		glycoprotein			Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport.
34407556	0	42	gly	Asialoglycoprotein	4:21	arg1	The Asialoglycoprotein Receptor	The Asialoglycoprotein Receptor				Fterm		Asialoglycoprotein			The Asialoglycoprotein Receptor Minor Subunit Gene Contributes to Pharmacokinetics of Factor VIII Concentrates in Hemophilia A. BACKGROUND The asialoglycoprotein receptor (ASGPR) binds with high affinity factor VIII (FVIII) through its N-linked oligosaccharides.
34407556	0	76	gly	asialoglycoprotein	143:160	arg1	The asialoglycoprotein receptor	The asialoglycoprotein receptor				Fterm		asialoglycoprotein			The Asialoglycoprotein Receptor Minor Subunit Gene Contributes to Pharmacokinetics of Factor VIII Concentrates in Hemophilia A. BACKGROUND The asialoglycoprotein receptor (ASGPR) binds with high affinity factor VIII (FVIII) through its N-linked oligosaccharides.
36959283	7	74	gly	sialylated	1423:1432	arg1	highly sialylated N-glycans				highly sialylated N-glycans						Furthermore, highly sialylated N-glycans are identified and structurally characterized, and untreated sialic acid-linkage isomers are resolved in a single CZE-MS analysis.
37266972	9	9	gly	site	1193:1196	arg1	the N-glycans			site	the N-glycans					site	More than 90% of the N-glycans at the conserved Asn297 site were deglycosylated.
37266972	9	67	gly	deglycosylated	1203:1216	arg1	the N-glycans				the N-glycans						More than 90% of the N-glycans at the conserved Asn297 site were deglycosylated.
37202422	2	10	part_of	Fc	289:290	arg1	the Fc domain	Fc		the Fc domain		Cterm	Site	Fc		domain	This glycan contributes to the structural organization of the Fc domain and determines its specificity for Fcγ receptors, thereby dictating distinct cellular responses.
35960654	10	56	gly	glycopeptide	1921:1932	arg2	the enhanced comprehensive quantitative intact glycopeptide analysis			the enhanced comprehensive quantitative intact glycopeptide analysis						glycopeptide	Taken together, we developed and optimized a strategy for the enhanced comprehensive quantitative intact glycopeptide analysis with DiLeu labeling, showing significant promise for identifying novel therapeutic targets or biomarkers in biological systems with a limited sample quantity.
35253441	4	17	part_of	region	709:714	arg1	the glycation sites	region		the glycation sites						sites	In addition, the glycation sites with the highest DSP in different samples were located in the irregular coil region of OVA.
36211377	6	20	gly	sialylated	1082:1091	arg1	sialylated N glycans				sialylated N glycans						The most significant alteration of the IgG N-glycome was present 8 weeks after the subjects underwent an LCD, a statistically significant decrease of agalactosylated and the increase of sialylated N glycans.
36585837	11	42	part_of	protein	1629:1635	arg1	14 sites	protein		14 sites		Fterm	Site	protein		sites	PIGR (polymeric immunoglobulin receptor) was the most glycosylated protein with 14 sites identified.
36252443	12	39	gly	glycoproteins	2109:2121	arg1	glycoproteins' biological roles	glycoproteins' biological roles				Fterm		glycoproteins			EndoBI-1 can be considered a promising enzyme for the release of N-glycans and their protein backbone in the native form, which will enable effective glycan release and will facilitate subsequent investigations to reveal their contribution to glycoproteins' biological roles.
36925056	0	42	gly	N-glycosylation	0:14	arg1	serum proteins	serum proteins				Fterm		proteins			N-glycosylation of serum proteins in adult type 1 diabetes mellitus exposes further changes compared to children at the disease onset.
35871894	7	16	gly	glycoproteins	1081:1093	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In this manuscript, we present a step-by-step workflow to build and perform MD analysis of glycoproteins focusing on the SPIKE glycoprotein of SARS-CoV-2 to appraise the impact of glycans in structure stabilization and antibody occlusion.
34972858	7	107	gly	glycoproteins	1302:1314	arg1	other glycoproteins	other glycoproteins				Fterm		glycoproteins			To address this challenge, we have constructed a detailed InstantPC-labeled glycan glucose unit (GU) database through analysis of human serum and a variety of other glycoproteins from various sources.
36935145	4	24	gly	N-glycosite	1340:1350	arg2	comprehensive N-glycosite			comprehensive N-glycosite						N-glycosite	Here we report our glycoproteome-wide qualitative N-glycoproteomics characterization of GoGs in breast cancer Adriamycin drug resistance (ADR) cells (MCF-7/ADR) and cancer stem cells (MCF-7/ADR CSCs); comprehensive N-glycosite and N-glycan structure information at the intact N-glycopeptide level were reported.
36935145	4	62	gly	N-glycopeptide	1401:1414	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	Here we report our glycoproteome-wide qualitative N-glycoproteomics characterization of GoGs in breast cancer Adriamycin drug resistance (ADR) cells (MCF-7/ADR) and cancer stem cells (MCF-7/ADR CSCs); comprehensive N-glycosite and N-glycan structure information at the intact N-glycopeptide level were reported.
31941975	2	43	gly	glycoproteins	341:353	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Fucosylation of glycoproteins plays an important role for structural stability and function of N-linked glycoproteins.
31941975	2	61	gly	glycoproteins	253:265	arg1	Fucosylation of glycoproteins				Fucosylation of glycoproteins						Fucosylation of glycoproteins plays an important role for structural stability and function of N-linked glycoproteins.
33085102	0	64	gly	glycoproteins	48:60	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Isolation and identification of O- and N-linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth.
35945033	7	10	gly	glycoproteins	1678:1690	arg1	horseradish peroxidase	horseradish peroxidase				Fterm		peroxidase			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	10	gly	glycoproteins	1678:1690	arg1	the glycoproteins horseradish peroxidase and bovine lactoferrin	the glycoproteins horseradish peroxidase and bovine lactoferrin				Fterm		glycoproteins			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	16	gly	deglycosylate	1660:1672	arg1	horseradish peroxidase	horseradish peroxidase				Fterm		peroxidase			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
35945033	7	16	gly	deglycosylate	1660:1672	arg1	the glycoproteins horseradish peroxidase and bovine lactoferrin	the glycoproteins horseradish peroxidase and bovine lactoferrin				Fterm		glycoproteins			Recombinant PNGase Rc was able to deglycosylate the glycoproteins horseradish peroxidase and bovine lactoferrin significantly faster than PNGase Dj (10 min vs. 6 h).
32417172	1	44	gly	O-glycosylation	183:197	arg1	secretory proteins	secretory proteins				Fterm		proteins			BACKGROUND Mucin-type O-glycosylation (referred to as O-GalNAc glycosylation) is the most abundant O-glycosylation on membrane and secretory proteins.
34726173	2	46	part_of	SDR	328:330	arg1	the long-chain SDR domain	SDR		the long-chain SDR domain		PUBTATOR	Site	SDR	8436	domain	The O-linked glycosylation of the long-chain SDR domain mediated by SdgB and SdgA is a key virulence factor that protects the adhesive SDR proteins against host proteolytic attack in order to promote successful tissue colonization, and has also been implicated in staphylococcal agglutination, which leads to sepsis and an immunodominant epitope for a strong antibody response.
35320529	2	47	gly	modified	204:211	arg1	Most human lymphocyte membrane receptors AND diverse glycan structures	Most human lymphocyte membrane receptors			diverse glycan structures	Fterm		receptors			Most human lymphocyte membrane receptors are modified by diverse glycan structures, and functional studies have indicated that a family of glycan-binding proteins, galectins, can significantly modulate lymphocyte development and function by interacting with these glycans.
35934376	0	91	gly	glycoprotein	80:91	arg1	ultrafast glycoprotein immobilization	ultrafast glycoprotein immobilization				Fterm		glycoprotein			In-depth profiling of urinary N-glycome in diabetic kidney disease by ultrafast glycoprotein immobilization for glycan extraction (UltraGIG).
34611867	4	26	gly	sialylated	687:696	arg1	galactosylated, agalactosylated, or sialylated glycoforms				galactosylated, agalactosylated, or sialylated glycoforms						Here we describe protocols that can be utilized to produce preferred glycoforms that include galactosylated, agalactosylated, or sialylated glycoforms following isolation of a mAb.
36580234	5	4	gly	sialylated	812:821	arg1	sialylated glycans				sialylated glycans						Results revealed that bisected GlcNAc, core fucosylated, and sialylated glycans (e.g., HexNAc4Hex5Fuc1Neu5Ac1, N4H5F1S1) were increased in M1 and M2 macrophages, especially in the latter.
36233110	5	49	gly	N-glycosylation	1152:1166	arg2	the five N-glycosylation sites			the five N-glycosylation sites						sites	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
36233110	5	52	gly	levels	1070:1075	arg1	the two sites			the two sites	the two sites		Site			sites	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
36233110	5	52	gly	levels	1070:1075	arg1	the VEGFR-2 domain			the VEGFR-2 domain	the VEGFR-2 domain		Site			domain	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
33006605	1	47	gly	glycoprotein	127:138	arg1	The spike (S) glycoprotein	The spike (S) glycoprotein				Fterm		glycoprotein			The spike (S) glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the binding to the permissive cells.
31657490	0	82	gly	glycopeptides	89:101	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	Postsynthesis of zwitterionic hydrophilic composites for enhanced enrichment of N-linked glycopeptides from human serum.
37331167	3	46	gly	glycoprotein	594:605	arg1	a glycoprotein α-1,3 mannosidase	a glycoprotein α-1,3 mannosidase				Fterm		glycoprotein			Through functional genomic analysis, we identified a glycoprotein α-1,3 mannosidase and named it MA3.
35405095	3	45	part_of	carrying	469:476	arg1	proteins AND densely O-glycosylated domains	proteins		densely O-glycosylated domains		Fterm	Site	proteins		domains	In particular, the ability to degrade host mucins, a class of proteins carrying densely O-glycosylated domains, provides a competitive advantage in the sustained colonization of niche mucosal environments.
35773089	11	27	part_of	IgG	2253:2255	arg1	aberrant IgG glycome composition	IgG		aberrant IgG glycome composition		PUBTATOR	Site	IgG	668542	position	The magnitude of observed changes is associated with the severity of the disease, indicating that aberrant IgG glycome composition or changes in IgG glycosylation may be an important molecular mechanism in COVID-19.
36385894	4	92	gly	glycoprotein	617:628	arg1	The N-glycan and glycoprotein profiles				The N-glycan and glycoprotein profiles						The N-glycan and glycoprotein profiles of a cohort (n = 26 patients, n = 22 age- and gender-matched) of lung cancer patients were analyzed and compared to identify potential N-glycan and glycopeptide serum biomarkers using nano-QToF-MS/MS and ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry dynamic multiple monitoring methods, respectively.
35696078	7	19	gly	glycosylation	959:971	arg2	the glycosylation sites			the glycosylation sites						sites	With the development of glycan-related databases and a glycan repository, bioinformatics approaches have attempted to predict the glycosylation pathway and the glycosylation sites on proteins.
36388954	3	2	gly	glycoprotein	738:749	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			The model was able to accommodate site-specific N-glycan processing and we identified phosphorylated glycan structures of the mannose-6-phosphate pathway responsible for the lysosomal sorting of the glycoprotein.
32714760	6	16	gly	glycosylation	759:771	arg1	ZP proteins	ZP proteins				Fterm		proteins			Mutant oocytes have a thin and fragile zona pellucida (ZP) due to the reduction in glycosylation of ZP proteins, and display poor developmental competence after fertilization in vitro.
36478308	12	94	gly	glycosylation	1753:1765	arg1	both spike protein variants	both spike protein variants				Fterm		variants			CONCLUSION Our data clearly shows that the overall glycosylation pattern of both spike protein variants was highly similar from batch-to-batch, and between materials produced at different manufacturing facilities.
35234395	0	12	part_of	glucosyltransferase	42:60	arg1	the 182 site	glucosyltransferase		the 182 site		Fterm	Site	glucosyltransferase		site	[Engineering the 182 site of cyclodextrin glucosyltransferase for glycosylated genistein synthesis].
36870092	10	24	gly	N-glycopeptide	1519:1532	arg2	down-regulated intact N-glycopeptide			N-glycopeptide	bisecting GlcNAc					N-glycopeptide	Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36870092	10	24	gly	N-glycopeptide	1519:1532	arg2	down-regulated intact N-glycopeptide			N-glycopeptide	β1,6-branching N-glycan					N-glycopeptide	Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36870092	10	41	gly	N-glycopeptide	1609:1622	arg2	up-regulated intact N-glycopeptide			N-glycopeptide	bisecting GlcNAc					N-glycopeptide	Annotation of protein-protein interaction and biological processes among others of DEGPs were finally carried out; down-regulated intact N-glycopeptide with bisecting GlcNAc from p38-interacting protein and up-regulated intact N-glycopeptide with β1,6-branching N-glycan from integrin beta-5 were found.
36529080	9	32	part_of	variants	1338:1345	arg1	a new N-linked glycosylation site	variants		a new N-linked glycosylation site		Fterm	Site	variants		site	we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36529080	9	56	part_of	present	1322:1328	arg2	some variants AND a new N-linked glycosylation site	some variants		a new N-linked glycosylation site		Fterm	Site	variants		site	we found that (1) a new N-linked glycosylation site is present in some variants, (2) the glycosylation of Dectin-1 plays an important role in the fate of Dectin-1 and its localization in the cells, and (3) the glycosylation is related to the amount of ingestion of the complex.
36095241	0	22	part_of	SARS-CoV-2	28:37	arg1	SARS-CoV-2 Homogeneous O-Linked Glycopeptides	SARS		SARS-CoV-2 Homogeneous O-Linked Glycopeptides		OGER	Site	SARS	P49591	Glycopeptides	Chemoenzymatic Synthesis of SARS-CoV-2 Homogeneous O-Linked Glycopeptides for Exploring Their Inhibition Functions.
34154738	9	23	gly	fucosylation	1409:1420	arg1	the antibody glycan				the antibody glycan						High expressing RMD clones reduced the fucosylation of the antibody glycan to as low as 16%.
35775947	1	54	gly	glycoproteins	345:357	arg1	the mucin-like glycoproteins	the mucin-like glycoproteins				Fterm		glycoproteins			Two important activities take place in the surface of Trypanosoma cruzi, the agent of Chagas disease: the trans-sialidase (TcTS) catalyzes the transfer of sialic acid from the host glycoconjugates to the mucin-like glycoproteins from the parasite and the presence of lytic antibodies recognize the epitope α-Galp(1 → 3)-β-Galp(1 → 4)-α-GlcNAcp.
35335137	10	45	gly	pro-inflammatory	1224:1239	arg1	the pro-inflammatory tetra-antennary plasma glycans			pro	the pro-inflammatory tetra-antennary plasma glycans					pro	We also detected a substantial increase in the pro-inflammatory tetra-antennary plasma glycans in bilateral AMD (7.90 (2.94-20.95)).
36766693	5	28	gly	mannosylated	1202:1213	arg1	mannosylated glycoproteins	mannosylated glycoproteins				Fterm		glycoproteins			Altered glycan structures composed of mannose residues are expected to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly the entire cascade of events linked to this lectin.
36766693	5	67	gly	glycoproteins	1215:1227	arg1	mannosylated glycoproteins	mannosylated glycoproteins				Fterm		glycoproteins			Altered glycan structures composed of mannose residues are expected to affect the reactivity of mannosylated glycoproteins with mannose-binding lectin and possibly the entire cascade of events linked to this lectin.
34778211	8	36	gly	glycopeptides	1332:1344	arg2	7,127 unique N-linked glycosite-containing intact glycopeptides			7,127 unique N-linked glycosite-containing intact glycopeptides						glycopeptides	A total of 7,127 unique N-linked glycosite-containing intact glycopeptides (IGPs), 928 glycosites, and 442 glycoproteins were identified from FUT8KO and WT CHO cells.
34778211	8	49	gly	glycosites	1358:1367	arg2	928 glycosites			928 glycosites						glycosites	A total of 7,127 unique N-linked glycosite-containing intact glycopeptides (IGPs), 928 glycosites, and 442 glycoproteins were identified from FUT8KO and WT CHO cells.
34778211	8	56	gly	glycoproteins	1378:1390	arg1	442 glycoproteins	442 glycoproteins				Fterm		glycoproteins			A total of 7,127 unique N-linked glycosite-containing intact glycopeptides (IGPs), 928 glycosites, and 442 glycoproteins were identified from FUT8KO and WT CHO cells.
35871410	9	72	gly	attached	1360:1367	arg2	invertase AND N-glycans	invertase			N-glycans	Fterm		invertase			In addition, the length of N-glycans attached to invertase was evaluated in the Δmnt1 strain.
35293727	7	83	gly	fragment	1323:1330	arg1	glycan ISD fragment ions				glycan ISD fragment ions						Three glycoconjugate vaccine candidates, obtained from the bioconjugation of the O-antigen polysaccharides from E. coli serotypes O2, O6A, and O25B with the genetically detoxified exotoxin A from Pseudomonas aeruginosa, were analyzed by MALDI-in-source decay (ISD) FT-ICR MS. Protein and glycan ISD fragment ions were selectively detected using 1,5-diaminonaphtalene and a 2,5-dihydroxybenzoic acid/2-hydroxy-5-methoxybenzoic acid mixture (super-DHB) as a MALDI matrix, respectively.
35397991	6	54	gly	enzyme	1390:1395	arg1	the end product N-glycans	enzyme			the end product N-glycans	Fterm		enzyme			The expression profile of GlcNAc extension-related genes revealed that the enzymes contributing to the hydrolysis of GlcNAc showed stage-specific expressions, thereby resulting in accumulations of the end product N-glycans of the enzyme.
34452239	7	63	gly	glycopeptide	1524:1535	arg2	the glycopeptide			the glycopeptide						glycopeptide	Advanced organic solution spray drying was used to engineer the particles and produce spray-dried powders (SD) of both the peptide and the glycopeptide, as well as co-spray-dried powders (co-SD) with the non-reducing sugar and pharmaceutical excipient, trehalose.
36565991	8	26	gly	glycosylation	1191:1203	arg2	viral glycosylation sites			viral glycosylation sites						sites	These observations suggest a mechanism whereby mutations affecting viral glycosylation sites have a structural impact across the protein surface.
35166741	3	15	part_of	peptides	578:585	arg1	Cys residues	peptides		Cys residues						Cys residues	Peptide thioesters and peptides with Cys residues at their N-terminals are necessary intermediates to perform native chemical ligation.
36892535	4	16	part_of	MUC1	613:616	arg1	homogeneously O-glycosylated MUC1 peptides	MUC1		homogeneously O-glycosylated MUC1 peptides		OGER	Site	MUC1	P15941	peptides	To better understand the role of MUC1 glycosylation in interaction and adhesion of cancer cells, we prepared a panel of homogeneously O-glycosylated MUC1 peptides using a quantitative chemoenzymatic approach.
37156312	5	45	gly	O-glycosites	729:740	arg2	O-glycosites			O-glycosites						O-glycosites	This study constructed a prediction model based on feature fusion for O-glycosites linked to the threonine residues in Homo sapiens.
36373229	2	7	gly	glycoproteins	324:336	arg1	only glycoproteins	only glycoproteins				Fterm		glycoproteins			O-Glycoprotease substrates range from any O-glycoprotein (albeit with specific O-glycan modifications) to only glycoproteins harboring specific O-glycosylated sequence motifs, such as those found in mucin domains.
36373229	2	60	gly	O-glycoprotein	255:268	arg1	any O-glycoprotein	O-glycoprotein			specific O-glycan modifications	Fterm		O-glycoprotein			O-Glycoprotease substrates range from any O-glycoprotein (albeit with specific O-glycan modifications) to only glycoproteins harboring specific O-glycosylated sequence motifs, such as those found in mucin domains.
36252443	11	77	gly	glycoproteins	1764:1776	arg1	the native glycoproteins	the native glycoproteins				Fterm		glycoproteins			Thirty-two and fifteen distinct compositions were released from the native glycoproteins by EndoBI-1 and PNGase F, respectively, as characterized by advanced mass spectrometry.
37162352	13	66	gly	glycosylation	1701:1713	arg2	the glycosylation sites			the glycosylation sites						sites	The function of O-glycosylation is likely related to protein folding and stability; therefore, the finding of the glycosylation sites has relevance for studies involving expression of the proteins.
36252443	5	92	gly	glycoprotein	843:854	arg1	glycoprotein denaturation	glycoprotein denaturation				Fterm		glycoprotein			However, for conventional enzymatic and chemical deglycosylation strategies to work efficiently, glycoprotein denaturation is required, which alters the protein native shape, hindering further investigations of its biological roles.
35388686	3	40	gly	glycopeptides	734:746	arg2	glycopeptides			glycopeptides						glycopeptides	The method is applied to study glycan compositions based on analysis of glycopeptides from proteolytic digestion of recombinant human coronaviruse spike proteins from SARS-CoV-2 and HKU1.
36903897	4	46	gly	glycoproteins	912:924	arg1	AGP fusion glycoproteins	AGP fusion glycoproteins				Fterm		glycoproteins			In addition, this work confirms the presence of β-1,6-linkage on the galactan backbone identified previously in AGP fusion glycoproteins expressed in tobacco suspension culture.
36746580	5	7	gly	sialylated	989:998	arg1	sialylated branching structures				sialylated branching structures						Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36746580	5	62	gly	core-α1,6-fucosylated	942:962	arg1	core-α1,6-fucosylated structures				core-α1,6-fucosylated structures						Moreover, human MFGM glycoproteins with core-α1,6-fucosylated structures and Lewis and sialylated branching structures play a role in the biological processes of antigen processing and presentation.
36436856	0	25	gly	glycoproteins	107:119	arg1	human milk glycoproteins	human milk glycoproteins				Fterm		glycoproteins			Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.
36436856	0	36	gly	levels	71:76	arg1	human milk glycoproteins	glycoproteins			levels	Fterm		glycoproteins			Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.
35092134	6	39	gly	glycosylated	908:919	arg1	most sites			most sites						sites	The results indicated that most sites were fully glycosylated and that the dominant population was the complex type.
36972173	6	32	gly	sites	822:826	arg1	the N-terminal domain			the N-terminal domain	the N-terminal domain		Site			domain	However, there are significant differences in the processing state at glycan sites in the N-terminal domain, such as N165.
34726173	7	84	part_of	SDR	1764:1766	arg1	the SDR domain	SDR		the SDR domain		PUBTATOR	Site	SDR	8436	domain	Overall, these findings not only elucidate the putative role of the three domains of SdgB in recognizing donor and acceptor substrates, but also provide new mechanistic insights into glycosylation of the SDR domain, which can serve as a starting point for the development of antibacterial drugs against staphylococcal infections.
36290159	9	77	gly	fucosylated	3053:3063	arg1	(iv) fucosylated glycans				(iv) fucosylated glycans						It can be summarized as follows: (i) high-mannosylated N-linked glycans (Con A reactivity) were present throughout the oviductal epithelium during the entire menstrual cycle and characteristically in the apical protrusions of non-ciliated cells of the ampulla during the preovulatory phase; (ii) sialoglycans with α2,3-linked sialic acids (MAL II binding) were expressed along the entire oviductal surface only during the preovulatory phase, whereas α2,6-linked ones (SNA affinity) were also detected in the surface of the luteal phase, although during the preovulatory phase they were characteristically found in the glycocalyx of the isthmus cilia, and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA) were found in the entire oviductal surface during all phases of the menstrual cycle; (iii) GalNAc terminating O-linked glycans (HPA staining) were mainly expressed in the entire oviducts of the luteal and preovulatory phases, and characteristically in the apical protrusions of the isthmus non-ciliated cells of the preovulatory phase; and (iv) fucosylated glycans with α1,2-linked fucose (LTA reactivity) occurred in the apical surface of fimbriae during the luteal phase, whereas α1,3/4-linked fucose (UEA I binders) were present in the apical protrusions of the ampulla non-ciliated cells and in the apical surface of isthmus during the preovulatory phase as well as in the isthmus apical surface of follicular-phase oviducts.
36870092	8	59	gly	N-glycopeptides	1113:1127	arg2	4777 intact N-glycopeptides			4777 intact N-glycopeptides						N-glycopeptides	Totally, 4777 intact N-glycopeptides were identified and N-glycan sequence structures among 2764 IDs were distinguished from their isomers by structure-diagnostic fragment ions.
36870092	8	72	gly	sequence	1158:1165	arg1	N-glycan sequence structures				N-glycan sequence structures						Totally, 4777 intact N-glycopeptides were identified and N-glycan sequence structures among 2764 IDs were distinguished from their isomers by structure-diagnostic fragment ions.
36740532	8	53	gly	O-glycosites	1245:1256	arg2	48 unique O-glycosites			48 unique O-glycosites						O-glycosites	In the analysis of the endogenous protein purified from platelets, we identified 48 unique O-glycosites and 1 N-glycosite.
36740532	8	69	gly	N-glycosite	1264:1274	arg2	1 N-glycosite			1 N-glycosite						N-glycosite	In the analysis of the endogenous protein purified from platelets, we identified 48 unique O-glycosites and 1 N-glycosite.
37216524	9	74	part_of	Cosmc	1204:1208	arg1	the transmembrane domain	Cosmc		the transmembrane domain		OGER	Site	Cosmc	Q96EU7	domain	This germline variant occurs within the transmembrane domain of Cosmc, resulting in dramatically reduced expression of the Cosmc protein.
36959936	7	83	gly	observed	1303:1310	arg1	the mammalian cell culture-derived protein AND the predominantly mature, complex glycans	the mammalian cell culture-derived protein			the predominantly mature, complex glycans	Fterm		protein			Complex-type glycans were almost entirely absent from the plant-derived material, which contrasted against the predominantly mature, complex glycans that were observed on the mammalian cell culture-derived protein.
37371476	5	38	gly	sialylated	1106:1115	arg1	sialylated tri- and tetra-antennary N-glycans				sialylated tri- and tetra-antennary N-glycans						The results from comparing pre-operative CRC to post-operative samples were in agreement with studies that identified a decrease in di-antennary structures with core fucosylation and an increase in sialylated tri- and tetra-antennary N-glycans in CRC patient sera.
37371476	5	42	gly	fucosylation	1074:1085	arg1	di-antennary structures				di-antennary structures						The results from comparing pre-operative CRC to post-operative samples were in agreement with studies that identified a decrease in di-antennary structures with core fucosylation and an increase in sialylated tri- and tetra-antennary N-glycans in CRC patient sera.
35995381	3	18	gly	glycopeptide	576:587	arg2	glycopeptide			glycopeptide						glycopeptide	This study aimed to profile human Tg N-glycosylation sites and their glycan contents by using high-throughput glyco-analytical strategies, including glycopeptide and glycan levels.
35995381	3	101	gly	N-glycosylation	464:478	arg2	human Tg N-glycosylation sites			human Tg N-glycosylation sites						sites	This study aimed to profile human Tg N-glycosylation sites and their glycan contents by using high-throughput glyco-analytical strategies, including glycopeptide and glycan levels.
36202042	2	50	gly	released	289:296	arg1	proteins AND the N-glycans	proteins			the N-glycans	Fterm		proteins			Various analytical techniques are used for the structural identification of the N-glycans released from proteins through various enzymatic and chemical methods.
37292769	2	4	gly	glycopeptide	267:278	arg2	isobaric glycopeptide structures			isobaric glycopeptide structures						glycopeptide	Despite its immense potential, the qualitative and quantitative analysis of isobaric glycopeptide structures remains one of the most daunting hurdles in the field of glycoproteomics.
37156312	11	63	gly	O-glycosites	1497:1508	arg2	O-glycosites			O-glycosites						O-glycosites	These results demonstrated the high competency of our predictor in identifying O-glycosites on threonine residues.
37071393	1	12	gly	N-glycosylated	148:161	arg1	Many secreted eukaryotic proteins	Many secreted eukaryotic proteins				Fterm		proteins			Many secreted eukaryotic proteins are N-glycosylated with oligosaccharides composed of a high-mannose N-glycan core and, in the specific case of yeast cell-wall proteins, an extended α-1,6-mannan backbone carrying a number of α-1,2- and α-1,3-mannose substituents of varying lengths.
33287410	5	10	gly	glycoproteins	980:992	arg1	the serum glycoproteins	the serum glycoproteins				Fterm		glycoproteins			Whole serum N-glycan profiling was carried out on 117 prostate cancer patients' serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins.
33287410	5	72	gly	released	956:963	arg1	the serum glycoproteins AND N-linked glycans	the serum glycoproteins			N-linked glycans	Fterm		glycoproteins			Whole serum N-glycan profiling was carried out on 117 prostate cancer patients' serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins.
32281790	4	3	gly	glycan	737:742	arg1	the Fc region			the Fc region	the Fc region		Site			region	Specifically, an azide-handle was introduced into the Fc region of a set of immunoglobulins using a two-step enzymatic reaction: (1) cleavage of N-linked glycan in the Fc region by a glycosidase and (2) conjugation of a chemically reactive linker (containing an azide functional handle) using a microbial transglutaminase.
36018613	10	51	gly	glycopeptide	983:994	arg1	the protonated glycopeptide [AT			glycopeptide	the protonated glycopeptide [AT					glycopeptide	Isomerization of the original pyranose, the protonated glycopeptide [AT(GalNAc)A+H]+, is predicted to be facile compared to direct dissociation, as is the glycosidic bond cleavage of the newly formed furanose form, i.e., furanose oxazolinium ion structures are predicted to predominate.
36018613	10	51	gly	glycopeptide	983:994	arg1	GalNAc			glycopeptide	GalNAc					glycopeptide	Isomerization of the original pyranose, the protonated glycopeptide [AT(GalNAc)A+H]+, is predicted to be facile compared to direct dissociation, as is the glycosidic bond cleavage of the newly formed furanose form, i.e., furanose oxazolinium ion structures are predicted to predominate.
36018613	7	47	gly	α-GalNAc-glycosylated	630:650	arg1	threonyl α-GalNAc-glycosylated peptides			threonyl α-GalNAc-glycosylated peptides							In the present article, we test the generality of the preceding findings by examining threonyl α-GalNAc-glycosylated peptides.
36136114	5	76	gly	glycosite	746:754	arg2	a given glycosite			glycosite	the glycan distribution					glycosite	Moreover, the type of glycosylation and the glycan distribution at a given glycosite depend on the host cell line and the expression system conditions that significantly impact safety and efficacy.
34939082	2	36	gly	glycopeptides	278:290	arg2	glycopeptides			glycopeptides						glycopeptides	This problem becomes acute when considering the increasing technical ability to profile large numbers of glycopeptides and glycans in recent studies.
36478308	3	100	gly	glycopeptide	487:498	arg2	glycopeptide libraries			glycopeptide libraries						glycopeptide	The development and use of glycopeptide libraries from previous developmental phases allowed for faster analysis than processing data sets from individual batches from first principles.
36581037	4	17	part_of	MBP-NPs	706:712	arg1	phenolic composition	MBP		phenolic composition		OGER	Site	MBP	P02686	position	During gastrointestinal digestion in vitro, the release characteristic and variation in phenolic composition of MBP-NPs were evaluated.
35211008	13	110	gly	α-1,6-fucosylation	2411:2428	arg1	isoproterenol-induced cardiac N-linked glycoproteins	isoproterenol-induced cardiac N-linked glycoproteins				Fterm		glycoproteins			Stachytine hydrochloride significantly reduced isoproterenol-induced cardiac N-linked glycoproteins with α-1,6-fucosylation.
35211008	13	121	gly	glycoproteins	2392:2404	arg1	isoproterenol-induced cardiac N-linked glycoproteins	glycoproteins			α-1,6-fucosylation	Fterm		glycoproteins			Stachytine hydrochloride significantly reduced isoproterenol-induced cardiac N-linked glycoproteins with α-1,6-fucosylation.
33789105	5	39	gly	fragment	812:819	arg1	the O-GlcNAc modification			fragment	the O-GlcNAc modification					fragment	Mechanistic studies show that Ogt directly interacts with Notch1 and catalyzes the O-GlcNAc modification of Notch TM/ICD fragment.
34452239	3	47	part_of	1-7	841:843	arg1	The related O-linked glycopeptide	Ang (1-7		The related O-linked glycopeptide		OGER	Site	Ang (1-7	Q15389	glycopeptide	The related O-linked glycopeptide, Asp-Arg-Val-Tyr-Ile-His-Ser-(O-β-D-Glc)-amide (PNA5), is a biousian revision of the native peptide hormone Ang (1-7) and shows enhanced stability in vivo and greater levels of brain penetration.
36036581	4	46	part_of	glycosites	812:821	arg1	S protein trimer	S protein trimer		glycosites		PUBTATOR	Site	S protein trimer	7448	glycosites	In addition to the common N-glycans as detected in previous studies, many uncommon glycosylation structures such as LacdiNAc structures, Lewis structures, Mannose 6-phosphate (M6P) residues, and bisected core structures were unambiguously mapped at a total of 20 glycosites in the S protein trimer and protomer.
37121976	1	37	gly	glycoprotein	105:116	arg1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1				Fterm		glycoprotein			The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
37121976	1	46	gly	ɑ-2-glycoprotein	131:146	arg1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1	The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1				Fterm		ɑ-2-glycoprotein 1			The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
37121976	1	46	gly	ɑ-2-glycoprotein	131:146	arg1	a multifunctional protein	a multifunctional protein				Fterm		protein			The serum glycoprotein leucine-rich ɑ-2-glycoprotein 1 (LRG1), primarily produced by hepatocytes and neutrophils, is a multifunctional protein that modulates various signaling cascades, mainly TGFβ signaling.
36253324	4	2	gly	enabling	817:824	arg1	intact LacNAc sites			intact LacNAc sites						sites	This strategy takes advantage of an operationally simple oxidation enzyme module by using galactose oxidase (GOase) to convert the LacNAc unit into oxidized C6'-aldehyde LacNAc sequence, which is not a good substrate for recombinant α1,3-FucT from Helicobacter pylori strain 26695 (Hpα1,3FucT), enabling the site-specific α1,3-fucosylation at intact LacNAc sites.
36253324	4	19	gly	strain	790:795	arg1	recombinant α1,3-FucT	strain			recombinant α1,3-FucT	Fterm		strain			This strategy takes advantage of an operationally simple oxidation enzyme module by using galactose oxidase (GOase) to convert the LacNAc unit into oxidized C6'-aldehyde LacNAc sequence, which is not a good substrate for recombinant α1,3-FucT from Helicobacter pylori strain 26695 (Hpα1,3FucT), enabling the site-specific α1,3-fucosylation at intact LacNAc sites.
36233110	2	28	gly	N-glycosylation	491:505	arg2	the five N-glycosylation sites			the five N-glycosylation sites						sites	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
36233110	2	35	gly	sites	507:511	arg1	one site			one site						site	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
36233110	2	35	gly	sites	507:511	arg1	two sites			sites						sites	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
37354227	8	72	gly	glycopeptides	1608:1620	arg2	glycopeptides			glycopeptides						glycopeptides	Additionally, we applied multiple digestion methods on each sample, and confirmed the results for individual glycosylation sites from different experiment conditions to improve the identification and quantification of glycopeptides.
37354227	8	102	gly	glycosylation	1499:1511	arg2	individual glycosylation sites			individual glycosylation sites						sites	Additionally, we applied multiple digestion methods on each sample, and confirmed the results for individual glycosylation sites from different experiment conditions to improve the identification and quantification of glycopeptides.
32820167	5	10	gly	positioned	669:678	arg1	O-glycans			position	O-glycans					position	We demonstrate that O-glycans positioned within the receptor binding motifs of members of the neuropeptide Y and glucagon families modulate receptor activation properties and substantially extend peptide half-lives.
34452239	0	61	part_of	Angiotensin	167:177	arg1	Advanced Spray Dried and Co-Spray Dried Angiotensin (1-7) Peptide	Angiotensin		Advanced Spray Dried and Co-Spray Dried Angiotensin (1-7) Peptide		Fterm		Angiotensin			Synthesis, Physicochemical Characterization, In Vitro 2D/3D Human Cell Culture, and In Vitro Aerosol Dispersion Performance of Advanced Spray Dried and Co-Spray Dried Angiotensin (1-7) Peptide and PNA5 with Trehalose as Microparticles/Nanoparticles for Targeted Respiratory Delivery as Dry Powder Inhalers.
36055406	2	10	part_of	GPI-attachment	502:515	arg1	C-terminal GPI-attachment sequence	GPI		C-terminal GPI-attachment sequence		OGER	Site	GPI	P06744	sequence	GPI-APs are synthesized in the cytosol as preproproteins, which contain an N-terminal signal sequence (SS), mature protein part, and C-terminal GPI-attachment sequence (GPI-AS), and translocated into the ER lumen where SS and GPI-AS are removed, generating mature GPI-APs.
36055406	2	7	part_of	contain	422:428	arg1	GPI-APs AND C-terminal GPI-attachment sequence	APs		sequence		OGER	Site	APs	P07288	sequence	GPI-APs are synthesized in the cytosol as preproproteins, which contain an N-terminal signal sequence (SS), mature protein part, and C-terminal GPI-attachment sequence (GPI-AS), and translocated into the ER lumen where SS and GPI-AS are removed, generating mature GPI-APs.
36055406	2	7	part_of	contain	422:428	arg1	preproproteins AND C-terminal GPI-attachment sequence	preproproteins		sequence		Fterm	Site	preproproteins		sequence	GPI-APs are synthesized in the cytosol as preproproteins, which contain an N-terminal signal sequence (SS), mature protein part, and C-terminal GPI-attachment sequence (GPI-AS), and translocated into the ER lumen where SS and GPI-AS are removed, generating mature GPI-APs.
35397991	1	38	gly	N-glycosylation	114:128	arg1	proteins	proteins				Fterm		proteins			N-glycosylation of proteins is an important post-translational modification in eukaryotic cells.
35616904	6	69	gly	glycoproteins	860:872	arg1	identified glycoproteins	identified glycoproteins				Fterm		glycoproteins			The majority of identified glycoproteins functioned in response to stimulus and immunity.
35939326	9	56	gly	epitope	1584:1590	arg1	the inner core trisaccharide key epitope				the inner core trisaccharide key epitope						The phosphate group of the inner core trisaccharide key epitope is very important for IgG recognition.
35651042	4	36	part_of	TFP	601:603	arg1	chemical composition	TFP		chemical composition		OGER	Site	TFP	Q9HCM9	position,	The results revealed that the reducing sugar content, chemical composition, molecular weight, rheological property, constituent monosaccharide, and FT-IR spectrum of TFP were not altered after the in vitro simulated digestion, indicating that it was indigestible under different simulated digestion conditions.
32898162	7	25	gly	domain	1160:1165	arg1	the second glycan			domain	the second glycan					domain	We propose that residues 227 and 230 and the second glycan are part of a C-terminal domain that acts as a linchpin for bank vole and mouse prion conversion.
34029329	5	48	gly	glycosylation	911:923	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	The amino acid sequences were assessed for their phylogenetic association, variable loop lengths and prevalence of potential N-linked glycosylation sites (pNLGS).
33343565	3	54	part_of	Fab	663:665	arg1	Fab regions	Fab		Fab regions		PUBTATOR	Site	Fab	2187	regions	Complement protein C1q binding of the IgG fusions was enhanced by: 1) antigen fusion to the IgG N-terminus; 2) removal of the IgG light chain or Fab regions; 3) addition of hexamer-inducing mutations in the IgG Fc; 4) adding a self-binding epitope tag to create recombinant immune complexes (RIC); or 5) producing IgG fusions in plants that lack plant-specific β1,2-linked xylose and α1,3-linked fucose N-linked glycans.
35854001	6	18	part_of	lectin	729:734	arg1	the lectin domain	structure of the lectin		the lectin domain		Fterm	Site	structure of the lectin	269181	domain	The crystal structure of the lectin domain shows structural similarity to a bacterial GlcNAc-binding lectin.
34957216	1	14	part_of	receptor	377:384	arg1	the receptor-binding domain	receptor		the receptor-binding domain		Fterm	Site	receptor		domain	Severe Acute respiratory syndrome coronavirus (SARS-CoV-1) attaches to the host cell surface to initiate the interaction between the receptor-binding domain (RBD) of its spike glycoprotein (S) and the human Angiotensin-converting enzyme (hACE2) receptor.
34957216	1	40	part_of	glycoprotein	308:319	arg1	the receptor-binding domain	glycoprotein		the receptor-binding domain		Fterm	Site	glycoprotein		domain	Severe Acute respiratory syndrome coronavirus (SARS-CoV-1) attaches to the host cell surface to initiate the interaction between the receptor-binding domain (RBD) of its spike glycoprotein (S) and the human Angiotensin-converting enzyme (hACE2) receptor.
34957216	1	47	part_of	receptor-binding	265:280	arg1	the receptor-binding domain	receptor		the receptor-binding domain		Fterm	Site	receptor		domain	Severe Acute respiratory syndrome coronavirus (SARS-CoV-1) attaches to the host cell surface to initiate the interaction between the receptor-binding domain (RBD) of its spike glycoprotein (S) and the human Angiotensin-converting enzyme (hACE2) receptor.
36827096	2	30	part_of	domain	491:496	arg1	WND motifs	domain		WND motifs						motifs	Here, we describe the cDNA cloning of lectin from the bivalve Glycymeris yessoensis (GYL), which encodes 161 amino acids and the C-type carbohydrate recognition domain (CRD) with EPN and WND motifs.
36827096	2	61	part_of	WND	517:519	arg1	WND motifs	WND		WND motifs		OGER	Site	WND	P35670	motifs	Here, we describe the cDNA cloning of lectin from the bivalve Glycymeris yessoensis (GYL), which encodes 161 amino acids and the C-type carbohydrate recognition domain (CRD) with EPN and WND motifs.
36244450	0	85	gly	N-glycosylation	0:14	arg1	mannose receptor	CD206			mannose receptor	PUBTATOR		CD206	4360		N-glycosylation of mannose receptor (CD206) regulates glycan binding by C-type lectin domains.
36598201	3	51	gly	N-glycosylation	429:443	arg2	two conserved N-glycosylation sites			two conserved N-glycosylation sites						sites	NSP4, a multifunctional protein involved in various viral replication processes, has two conserved N-glycosylation sites; however, the role of glycans remains elusive.
35421698	4	48	part_of	3,140 N-glycoproteins	731:751	arg1	3,713 N-glycosites	3,140 N-glycoproteins		3,713 N-glycosites		Fterm	Site	3,140 N-glycoproteins		3,713 N-glycosites	With target-decoy searches and spectrum-level FDR ≤ 1%, 5,687 N-glycopeptides from 3,713 N-glycosites of 3,140 N-glycoproteins were identified, which represents the currently most comprohensive profilling to our best knowledge.
36192063	5	29	part_of	TMT-labeled	854:864	arg1	TMT-labeled intact N-glycopeptides	TMT		TMT-labeled intact N-glycopeptides		OGER	Site	TMT	Q9NRR2	N-glycopeptides	TMT-labeled intact N-glycopeptides with and without sialic acid alkylamidation were subject to reversed-phase liquid chromatography-nano-electron spray ionization-tandem mass spectrometry (RPLC-nanoESI-MS/MS) analysis to provide comprehensive characterization of N-glycosylation with and without sialic acid at the intact N-glycopeptide level with structure and N-glycosite.
37156312	3	57	gly	O-glycosites	517:528	arg2	O-glycosites			O-glycosites						O-glycosites	Biochemical experiments to identify O-glycosites in batches are technically and economically demanding.
36838558	8	12	gly	glycosylated	1105:1116	arg1	the glycosylated peptides			the glycosylated peptides						peptides	Furthermore, structural information regarding the glycosylation site and glycan structure was obtained using tandem mass spectrometric (MS/MS) analysis of the glycosylated peptides after tryptic digestion.
36838558	8	39	gly	glycosylation	996:1008	arg2	the glycosylation site			the glycosylation site						site	Furthermore, structural information regarding the glycosylation site and glycan structure was obtained using tandem mass spectrometric (MS/MS) analysis of the glycosylated peptides after tryptic digestion.
36637420	0	79	gly	N-glycosylation	0:14	arg1	Asn-57			Asn-57						Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	protease inhibitory activity	protease		Asn-57		Fterm		protease		Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	the correct HAI-2 protein folding	HAI-2 protein		Asn-57		PUBTATOR		HAI-2 protein	10653	Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
36637420	0	79	gly	N-glycosylation	0:14	arg1	protease inhibitory activity	protease		Asn-57		Fterm		protease		Asn-57	N-glycosylation on Asn-57 is required for the correct HAI-2 protein folding and protease inhibitory activity.
32273875	6	58	gly	removal	1175:1181	arg3	mannose-ending glycans AND N2118			N2118	mannose-ending glycans					N2118	However, removal of mannose-ending glycans at N2118 did not alter factor VIII endocytosis and presentation to CD4+ T cells by mouse antigen-presenting cells.
36813234	7	24	gly	glycoproteins	1279:1291	arg1	two structurally distinct glycoproteins	two structurally distinct glycoproteins				Fterm		glycoproteins			We found that the expression of GnT-III consistently decreased chemoresistance for doxorubicin and dasatinib, as well as activation of the NF-κB pathway by tumor necrosis factor α (TNFα), which binds to two structurally distinct glycoproteins, TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2), on the cell surface.
35848837	6	47	gly	attached	958:965	arg2	their target proteins AND asparagine (Asn)-linked and free glycans	their target proteins			asparagine (Asn)-linked and free glycans	Fterm		proteins			In this article, we present a method that can characterize the N-linked glycosylation system in Campylobacter jejuni by detecting asparagine (Asn)-linked and free glycans that are not attached to their target proteins.
36812846	2	17	gly	glycoproteins	327:339	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Recently, free oligomannose-type glycans generated by the hydrolysis of glycoproteins or dolichol pyrophosphate-linked oligosaccharides were recognized as important signals for immunogenicity.
31941975	4	23	gly	N-glycopeptides	637:651	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Here, we report for the first time the classification of N-glycopeptides as core- and outer-fucosylated types using tandem mass spectrometry (MS/MS) and machine learning algorithms such as the deep neural network (DNN) and support vector machine (SVM).
31941975	4	56	gly	outer-fucosylated	666:682	arg1	outer-fucosylated types				outer-fucosylated types						Here, we report for the first time the classification of N-glycopeptides as core- and outer-fucosylated types using tandem mass spectrometry (MS/MS) and machine learning algorithms such as the deep neural network (DNN) and support vector machine (SVM).
36322419	7	85	part_of	contains	1604:1611	arg1	CapGH5_57 AND a tryptophan residue	CapGH5_57		a tryptophan residue		Cterm	AminoAcid	CapGH5_57	Q8K2I4	tryptophan residue	Supporting this, CapGH5_57 contains a tryptophan residue adjacent and perpendicular to the cleavage site, indicative of an anchoring site for a substrate with a substitution at the -1 glycosyl moiety.
37319314	3	12	gly	glycopeptide	651:662	arg2	glycopeptide isomer ratios			glycopeptide isomer ratios						glycopeptide	Here, we developed an LC-MS/MS-based workflow for determining glycopeptide isomer ratios.
37202422	3	19	gly	glycoproteins	491:503	arg1	highly-related, but non-equivalent glycoproteins	highly-related, but non-equivalent glycoproteins				Fterm		glycoproteins			The variable construction of this glycan structure leads to highly-related, but non-equivalent glycoproteins known as glycoforms.
33657401	5	30	gly	protein	867:873	arg1	O-GlcNAcylation-dependent binding	protein			O-GlcNAcylation-dependent binding	Fterm		protein			We determined that protein nuclear import by O-GlcNAcylation-dependent binding of cargo protein to importin α might be a general mechanism in cells.
36968546	8	21	gly	sialylated	1277:1286	arg1	a sialylated trisaccharide				a sialylated trisaccharide						Similarly, O-glycosylation with a sialylated trisaccharide had a negative impact on TMPRSS2 cleavage.
34636989	7	67	part_of	XBP1-binding	1157:1168	arg1	XBP1-binding motifs	XBP1		XBP1-binding motifs		PUBTATOR	Site	XBP1	7494	motifs	Using next-generation sequencing followed by bioinformatic analysis of XBP1-binding motifs, we defined an XBP1 regulatory network and identified XBP1 as a repressor of PUMA (a proapoptotic gene) and IRE1 mRNA expression during the UPR.
35304921	8	77	gly	found	984:988	arg1	β1 integrin AND An O-linked HexNAc	integrin		domain	An O-linked HexNAc	Fterm		integrin		domain	An O-linked HexNAc was found in an EGF-like domain of β1 integrin.
33801653	1	20	gly	attachment	203:212	arg2	Ser/Thr residues AND GlcNAc			Ser/Thr residues	GlcNAc					residues	Protein O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine (GlcNAc) to the hydroxyl groups of Ser/Thr residues on numerous nucleocytoplasmic proteins.
33801653	1	20	gly	attachment	203:212	arg2	Ser/Thr residues AND N-acetylglucosamine			Ser/Thr residues	N-acetylglucosamine					residues	Protein O-GlcNAcylation is a dynamic post-translational modification involving the attachment of N-acetylglucosamine (GlcNAc) to the hydroxyl groups of Ser/Thr residues on numerous nucleocytoplasmic proteins.
36959210	2	44	gly	glycoproteins	302:314	arg1	gel-forming glycoproteins	gel-forming glycoproteins				Fterm		glycoproteins			One important, but understudied, factor is the natural host environment: mucus, which contains gel-forming glycoproteins (mucins) that display hundreds of glycan structures with potential regulatory function.
35802832	1	21	gly	glycopeptide	275:286	arg2	the glycopeptide			the glycopeptide						glycopeptide	Casein glycomacropeptide carries various O-glycan modifications, which, together with variations in the amino acid composition of the glycopeptide, may result in different biological activities.
35802832	1	25	gly	carries	166:172	arg1	Casein glycomacropeptide AND various O-glycan modifications			Casein glycomacropeptide	various O-glycan modifications					glycomacropeptide	Casein glycomacropeptide carries various O-glycan modifications, which, together with variations in the amino acid composition of the glycopeptide, may result in different biological activities.
35766466	3	71	gly	glycoproteins	563:575	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			A novel MALDI imaging mass spectrometry workflow has been recently developed to analyze these features of N-glycosylation through the utilization of endoglycosidase enzymes to cleave N-glycans from associated glycoproteins.
34611869	4	15	part_of	regions	816:822	arg1	hydrophobic and aromatic residues	regions		hydrophobic and aromatic residues						residues	To study antibody aggregation in silico we used the online software TANGO and AGGRESCAN to identify aggregation prone regions (APR) in the antibodies and the influence of the Fc glycans on hydrophobic and aromatic residues present in the APRs.
35316032	5	78	part_of	Fab	1315:1317	arg1	both Fc and Fab domains	Fab		both Fc and Fab domains		PUBTATOR	Site	Fab	2187	domains	The chemoenzymatic approach was highly specific for Fc glycan remodeling when both Fc and Fab domains were glycosylated, as exemplified by the selective Fc-glycan remodeling of cetuximab.
37162352	14	34	gly	O-glycosylated	1867:1880	arg1	Six proteins	Six proteins				Fterm		proteins			Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated, which may impact the structure and function of these components.
34691043	7	16	part_of	HA	1013:1014	arg1	the HA receptor binding site	HA		the HA receptor binding site		Cterm	Site	HA		site	Here, we asked whether cysteine stabilization of the hyperglycosylated HA trimer could reverse this immunodominance by preventing access to the interface epitope and focus responses to the HA receptor binding site (RBS).
36585837	11	77	gly	glycosylated	1616:1627	arg1	the most glycosylated protein	the most glycosylated protein				Fterm		protein			PIGR (polymeric immunoglobulin receptor) was the most glycosylated protein with 14 sites identified.
36985724	6	43	gly	glycoproteins	1110:1122	arg1	therapeutic glycoproteins	glycoproteins			N-glycan engineering	Fterm		glycoproteins			The platform can find broad applications in N-glycan engineering of therapeutic glycoproteins.
32357974	9	51	part_of	HLA-I	1706:1710	arg1	HLA-I bound asparagine	HLA		HLA-I bound asparagine		OGER	AminoAcid	HLA		asparagine	This indicates that a large proportion of HLA-I bound asparagine deamidated peptides are generated from formerly glycosylated proteins that have undergone deglycosylation via the ER-associated protein degradation (ERAD) pathway.
32028683	1	16	part_of	Amyloid-β	102:110	arg1	Amyloid-β (Aβ) peptides	Amyloid-β 		Amyloid-β (Aβ) peptides		PUBTATOR	Site	Amyloid-β 	351	peptides	Amyloid-β (Aβ) peptides play a crucial role in the pathogenesis of Alzheimer's disease (AD).
35887202	5	36	part_of	MUC1	726:729	arg1	MUC1 O-glycopeptide	MUC1		MUC1 O-glycopeptide		OGER	Site	MUC1	P15941	O-glycopeptide	The aim of this study is to elucidate the mode of interaction between MY.1E12 and MUC1 O-glycopeptide by NMR, molecular dynamics (MD) and docking simulations.
36924942	5	6	gly	glycosylated	883:894	arg1	a conserved oligosaccharide core				a conserved oligosaccharide core						The N-terminal and C-terminal modules add alternating Kdo residues with β-(2→4) and β-(2→7) linkages, respectively, generating a conserved oligosaccharide core that is further glycosylated to produce diverse capsule structures.
32417172	9	53	part_of	sites	1331:1335	arg1	p53	p53		sites		OGER	Site	p53	P04637	sites	Ser121 residue was one of the glycosylated sites on p53.
35123071	6	3	part_of	glycoproteins	1039:1051	arg1	43 glycopeptides	glycoproteins		43 glycopeptides		Fterm	Site	glycoproteins		glycopeptides	A total of 41 glycoproteins with 43 glycopeptides were found to possess a bisecting GlcNAc, and 25 of these glycoproteins were reported to exhibit this type of modification for the first time.
35854001	2	7	gly	branch	348:353	arg1	the α1-3 mannose arm			the α1-3 mannose arm	the α1-3 mannose arm						N-Acetylglucosaminyltransferase-IVa (GnT-IVa, also designated as MGAT4A) forms a β1-4 GlcNAc branch on the α1-3 mannose arm in N-glycans.
35123071	7	61	gly	glycoproteins	1386:1398	arg1	bisecting GlcNAc modifications	glycoproteins			bisecting GlcNAc modifications	Fterm		glycoproteins			These results provide insights into the potential roles of bisecting GlcNAc modification in the human amniotic membrane, and can be beneficial to functional studies on glycoproteins with bisecting GlcNAc modifications and functional studies on immune suppression in human placenta.
36311785	2	28	gly	glycoprotein	467:478	arg1	yeast glycoprotein	yeast glycoprotein				Fterm		glycoprotein			In our previous study, we reported that supplementation of Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG) in combination synergistically improved antioxidant activities, serum lipid profile, and fatty acid metabolism in chicken.
32691273	5	71	gly	proteins	864:871	arg1	the O-GlcNAcylation	proteins			the O-GlcNAcylation	Fterm		proteins			First, we treated cells with glucosamine and PUGNAc to stimulate the O-GlcNAcylation of total proteins.
35566103	3	48	part_of	HPMC/MCC	595:602	arg1	HPMC/MCC bio-composite	MCC		HPMC/MCC bio-composite		OGER	Site	MCC		bio-composite	MCC at 5%, 10%, 20%, and 30% w/w was introduced into the HPMC matrix and the physical, barrier, thermal, and optical properties of the HPMC/MCC bio-composite (HMB) films were analyzed.
35597280	0	25	part_of	motif	35:39	arg1	thrombospondin-1	thrombospondin-1		motif		PUBTATOR	Site	thrombospondin-1	7057	motif	O-fucosylation stabilizes the TSR3 motif in thrombospondin-1 by interacting with nearby amino acids and protecting a disulfide bond.
35597280	0	34	part_of	TSR3	30:33	arg1	the TSR3 motif	TSR3		the TSR3 motif		PUBTATOR	Site	TSR3	115939	motif	O-fucosylation stabilizes the TSR3 motif in thrombospondin-1 by interacting with nearby amino acids and protecting a disulfide bond.
35995381	6	50	gly	20 N-glycosylation	1013:1030	arg2	20 N-glycosylation sites			20 N-glycosylation sites						sites	In total, 17 out of 20 N-glycosylation sites were characterized.
36827096	1	25	gly	glycoproteins	255:267	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			C-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders.
34186233	1	47	gly	residues	354:361	arg1	a single β-N-acetylglucosamine (GlcNAc) moiety			residues	a single β-N-acetylglucosamine (GlcNAc) moiety					residues	O-GlcNAcylation is a dynamic post-translational modification mediated by O-linked β-N-acetylglucosamine transferase (OGT) and O-GlcNAc hydrolase (OGA), that adds or removes a single β-N-acetylglucosamine (GlcNAc) moiety to or from serine/threonine residues of nucleocytosolic and mitochondrial proteins, respectively.
37354227	7	28	gly	types	1295:1299	arg1	later variants	variants			types	Fterm		variants			In comparison with our previously reported site-specific glycan analysis on the S-D614G and its ancestral protein, glycan types on later variants showed high similarity on the site-specific glycan content to S-D614G.
33244686	5	28	gly	glycopeptides	1317:1329	arg2	glycopeptides			glycopeptides						glycopeptides	Here, we reported an optimized experimental condition for IP-HILIC-MS where glycine is added in the TFA-containing mobile phases to enhance the MS detection sensitivity for glycopeptides up to ~ 50-fold by eliminating the ion-suppression effect of an ion-pairing agent while still retaining excellent separation capacity.
36740532	4	48	gly	glycosylation	610:622	arg2	GPIbα amino acid sites			GPIbα amino acid sites						sites	OBJECTIVES The aim of this study was to comprehensively analyze GPIbα amino acid sites of glycosylation (glycosites) and glycan structures.
36740532	4	51	gly	glycosites	625:634	arg2	glycosites			glycosites						glycosites	OBJECTIVES The aim of this study was to comprehensively analyze GPIbα amino acid sites of glycosylation (glycosites) and glycan structures.
35504880	4	1	gly	glycopeptides	571:583	arg2	the glycopeptides			the glycopeptides						glycopeptides	The binding of the glycopeptides to the enzyme is mainly driven by the GalNAc moiety while the peptide sequence provides optimal kinetic and binding parameters.
36311785	0	81	gly	glycoprotein	173:184	arg1	Yeast glycoprotein	Yeast glycoprotein				Fterm		glycoprotein			Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens.
34626388	6	65	gly	fucosylated	1426:1436	arg1	novel fucosylated GAG structures				novel fucosylated GAG structures						Moreover, fucose was detected in chondroitin sulfate, dermatan sulfate, and heparan sulfate, and both fucose and mannose were detected in chondroitin sulfate, suggesting the co-presence of other types of glycans or novel fucosylated GAG structures.
34626388	5	53	gly	observed	1093:1100	arg2	heparin AND galactosamine	heparin			galactosamine	Fterm		heparin			Glucosamine was found in both chondroitin sulfate and dermatan sulfate whereas galactosamine was observed in both heparan sulfate and heparin, indicating the cross contamination among different types of GAGs.
36095053	5	11	gly	N79	756:758	arg1	the N-glycans			N79	the N-glycans					N79	We show that the N-glycans at N79 have a requisite role in translocation of TREM2 to the cell surface, while the N-glycans at both N20 and N79 have a critical role in intracellular signal transduction.
36095053	5	45	gly	N79	865:867	arg1	the N-glycans			N20 and N79	the N-glycans					N20 and N79	We show that the N-glycans at N79 have a requisite role in translocation of TREM2 to the cell surface, while the N-glycans at both N20 and N79 have a critical role in intracellular signal transduction.
36095053	5	53	gly	N20	857:859	arg1	the N-glycans			N20 and N79	the N-glycans					N20 and N79	We show that the N-glycans at N79 have a requisite role in translocation of TREM2 to the cell surface, while the N-glycans at both N20 and N79 have a critical role in intracellular signal transduction.
36660462	5	88	part_of	sites	951:955	arg1	159 glycoproteins	glycoproteins		sites		Fterm	Site	glycoproteins		sites	A total of 789 glycopeptides were accurately quantified, which were derived from 204 glycosylated sites in 159 glycoproteins and were modified by 59 N-Linked glycans.
37287875	3	11	gly	N-glycoproteins	458:472	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			StrucGP is a glycan database-independent search engine for the structural interpretation of N-glycoproteins at the site-specific level.
35452121	2	48	gly	glycosylation	464:476	arg1	proteins	proteins				Fterm		proteins			Recent glycobiological research is painting a picture in which glycans represent a crucial structural and functional component of the majority of proteins, with alternative glycosylation of proteins and lipids being an important regulatory mechanism in many biological and pathological processes.
36870092	6	47	gly	N-glycopeptides	697:711	arg2	differential cell-surface intact N-glycopeptides			differential cell-surface intact N-glycopeptides						N-glycopeptides	Here, differential cell-surface intact N-glycopeptides in adriamycin (ADR)-resistant michigan breast cancer foundation-7 stem cells (MCF-7/ADR CSCs) relative to ADR-sensitive MCF-7 CSCs were analyzed with site- and structure-specific quantitative N-glycoproteomics.
33686291	3	33	gly	deglycosylation	457:471	arg1	a target protein	a target protein				Fterm		protein			Herein, we developed a nanobody-fused split O-GlcNAcase (OGA) as an O-GlcNAc eraser for selective deglycosylation of a target protein in cells.
34422587	1	2	gly	sialylated	169:178	arg1	mucin-type sialylated O-linked glycans				mucin-type sialylated O-linked glycans						BACKGROUND A recent study showed that mucin-type sialylated O-linked glycans could induce the increased expression of PD-L1 via binding to Siglec receptors.
36089065	2	26	gly	glycoprotein	434:445	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			N-glycans are processed in the secretory pathway to form varied ensembles of structures, and diversity at a single site on a glycoprotein is termed 'microheterogeneity'.
34274643	9	70	gly	glycoproteins	1558:1570	arg1	other intact glycoproteins	other intact glycoproteins				Fterm		glycoproteins			The separation and analysis methods that were developed could be used with relatively small amounts of AGP and can be adapted for use with other intact glycoproteins.
37156312	6	47	gly	glycosites	899:908	arg2	O-linked threonine glycosites			O-linked threonine glycosites						glycosites	In the training model, we collected and sorted out high-quality human protein data with O-linked threonine glycosites.
35604098	1	15	gly	glycoproteins	233:245	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycosylation of proteins is known to be essential for changing biological activity and stability of glycoproteins on the cell surfaces and in body fluids.
35604098	1	33	gly	Glycosylation	132:144	arg1	proteins	proteins				Fterm		proteins			Glycosylation of proteins is known to be essential for changing biological activity and stability of glycoproteins on the cell surfaces and in body fluids.
36329887	0	49	gly	Asparagine<sup>52</sup>	30:52	arg1	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans			Asparagine	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans					Asparagine	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans: Major Glycan Structural Consistency, Minor Glycan Variation in Abundance.
36329887	0	49	gly	Asparagine<sup>52</sup>	30:52	arg1	Minor Glycan Variation			Asparagine	Minor Glycan Variation					Asparagine	Human FSH Glycoform α-Subunit Asparagine<sup>52</sup> Glycans: Major Glycan Structural Consistency, Minor Glycan Variation in Abundance.
34691043	9	51	gly	cysteine	1269:1276	arg1	glycan engineering			cysteine	glycan engineering					cysteine	Thus, glycan engineering and cysteine stabilization are two strategies that can be used together to alter immunodominance patterns to HA.
35782731	6	57	gly	glycoprotein	874:885	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			Since the mentioned central region in the glycoprotein is not surrounded by glycans and is close to the heme, it is easily approachable to the solvent and substrate.
35506863	7	72	gly	fucosylated	837:847	arg1	complex and outer-arm fucosylated glycans				complex and outer-arm fucosylated glycans						Following rat SCI, complex and outer-arm fucosylated glycans decreased while oligomannose and hybrid structures increased.
33381907	2	60	gly	glycosylation	303:315	arg1	a cysteine			cysteine						cysteine	Bang-25 consists of the first 25 amino acids of the N-terminus of angiotensinogen (Aogen), with N-linked glycosylation on the 14th amino acid and a cysteine conjugated to the 18th amino acid.
35616904	2	81	gly	glycoproteins	268:280	arg1	these glycoproteins	these glycoproteins				Fterm		glycoproteins			However, the detailed information on these glycoproteins, particularly site-specific glycan structures, is still limited.
35616904	1	0	gly	glycoproteins	91:103	arg1	N-Linked glycoproteins	N-Linked glycoproteins				Fterm		glycoproteins			N-Linked glycoproteins are rich in seminal plasma, playing various essential roles in supporting sperm function and the fertilization process.
34911982	3	29	gly	region	618:623	arg1	N-linked glycans			region	N-linked glycans					region	During both types of immunizations, antigen-specific immunoglobulins G (IgGs) have been characterized by the reduced amount of fucose present on N-linked glycans of the fragment crystallizable (Fc) region.
32296440	2	54	part_of	SR-F1	327:331	arg1	recombinant SR-F1 ectodomain	SR-F1		recombinant SR-F1 ectodomain		PUBTATOR	Site	SR-F1	54558	ectodomain	We produced recombinant SR-F1 ectodomain and fragments deleted from the last 2 or 5 C-terminal epidermal growth factor-like modules and investigated their role in the binding of acetylated low density lipoprotein (AcLDL), complement C1q, and calreticulin (CRT).
35887202	6	20	part_of	MUC1	823:826	arg1	MUC1 O-glycopeptides	MUC1		MUC1 O-glycopeptides		PUBTATOR	Site	MUC1	P15941	O-glycopeptides	NMR titration using MUC1 O-glycopeptides suggests that the epitope is located within the O-linked glycan and near the O-glycosylation site.
36329887	4	136	part_of	receptor	787:794	arg1	the complete extracellular domain	FSH receptor		the complete extracellular domain		PUBTATOR	Site	FSH receptor	100769953	domain	Endoglycosidase F1-deglycosylated FSH bound to the complete extracellular domain of the FSH receptor crystallized as a trimeric complex.
36598201	3	30	part_of	has	411:413	arg1	a multifunctional protein AND two conserved N-glycosylation sites	a multifunctional protein		two conserved N-glycosylation sites		Fterm	Site	protein		sites	NSP4, a multifunctional protein involved in various viral replication processes, has two conserved N-glycosylation sites; however, the role of glycans remains elusive.
36598201	3	30	part_of	has	411:413	arg1	NSP4 AND two conserved N-glycosylation sites	NSP4		two conserved N-glycosylation sites		OGER	Site	NSP4	Q14B24	sites	NSP4, a multifunctional protein involved in various viral replication processes, has two conserved N-glycosylation sites; however, the role of glycans remains elusive.
35370997	3	13	gly	attached	630:637	arg1	the Fc fragment AND N-glycans			the Fc fragment	N-glycans					fragment	The IgG structure and its effector functions are strongly dependent on the composition of N-glycans attached to the Fc fragment, and the binding of antigens is regulated by Fab sugar moieties.
36781790	6	29	gly	glycosylation	1495:1507	arg2	site			site						site	Benefits of the glycomics-assisted glycoproteomics method, which can be readily applied to both simple and complex biological specimens such as protein extracts from cells, tissues, and bodily fluids (e.g., serum), include quantitative information of the protein carriers and site(s) of glycosylation, site occupancy, and the site-specific glycan structures directly from biological samples.
35662639	6	33	gly	glycoprotein	1574:1585	arg1	the glycoprotein subproteomes	the glycoprotein subproteomes				Fterm		glycoprotein			The different contents of lectin-enriched proteins underscore novel aspects of the variability of the glycoprotein subproteomes of Bothrops venoms and point to the role of distinct types of glycan chains in generating different venoms by closely related snake species.
35960654	2	28	gly	glycopeptides	420:432	arg2	glycopeptides			glycopeptides						glycopeptides	However, mass spectrometry (MS)-based glycoproteomic analysis is hindered by the low abundance and poor ionization efficiency of glycopeptides.
33904933	7	67	gly	glycosylated	1467:1478	arg1	the glycosylated epitope			the glycosylated epitope						epitope	Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, si-RNA silencing analysis, enzymatic deglycosylation, and tunicamycin treatment revealed that 12G5A recognized the glycosylated epitope on annexin A2 protein.
36014368	4	7	gly	O-glycosylation	707:721	arg2	numerous mucin-type O-glycosylation sites			numerous mucin-type O-glycosylation sites						sites	The SARS-CoV-2 S protein is heavily glycosylated with 22 predicted N-glycosylation consensus sites as well as numerous mucin-type O-glycosylation sites.
36014368	4	54	gly	N-glycosylation	644:658	arg2	22 predicted N-glycosylation consensus sites			22 predicted N-glycosylation consensus sites						sites	The SARS-CoV-2 S protein is heavily glycosylated with 22 predicted N-glycosylation consensus sites as well as numerous mucin-type O-glycosylation sites.
36252443	3	34	gly	glycoproteins	499:511	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			To date, the mechanisms, and contributions of glycans to the biological activities of glycoproteins remain to be elucidated.
36189205	2	19	gly	N-glycan	176:183	arg1	the Fc region			the Fc region	the Fc region		Site			region	The conserved N-glycan in the Fc region of IgG1 impacts interaction of IgG with FcγRs and the resulting effector functions, which has led to the design of antibody therapeutics with greatly improved antibody-dependent cell cytotoxicity (ADCC) activities.
35616904	7	33	gly	detected	1020:1027	arg1	seminal plasma glycoproteins AND heavy fucosylation	seminal plasma glycoproteins			heavy fucosylation	Fterm		glycoproteins			As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	70	gly	fucosylation	966:977	arg1	seminal plasma glycoproteins	seminal plasma glycoproteins				Fterm		glycoproteins			As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
35616904	7	71	gly	glycoproteins	1047:1059	arg1	seminal plasma glycoproteins	seminal plasma glycoproteins				Fterm		glycoproteins			As we reported in human spermatozoa, heavy fucosylation (fucose residues ≥6 per glycan) was also detected on seminal plasma glycoproteins such as clusterin and galectin-3-binding protein, which were involved in the immune response of biological processes and reactome pathways.
36813234	5	1	gly	P-glycoprotein	826:839	arg1	P-glycoprotein	P-glycoprotein				Fterm		P-glycoprotein			By contrast, the expression levels of both P-glycoprotein (P-gp) and its intracellular key regulator, NF-κB signaling, are significantly increased in K562/ADR cells.
32592613	6	5	part_of	IGFBP-3	1019:1025	arg1	the IGFBP-3 peptide	IGFBP-3		the IGFBP-3 peptide		OGER	Site	IGFBP-3	P17936	peptide	We show that both stereochemistry and amino acid identity are important determinants of the interaction between the IGFBP-3 peptide and HA and for the peptide's ability to exert its cytotoxic effects.
37040463	0	29	gly	Azido-Sialoglycoproteins	79:102	arg1	Clickable Azido-Sialoglycoproteins	Clickable Azido-Sialoglycoproteins				Fterm		Azido-Sialoglycoproteins			GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building Clickable Azido-Sialoglycoproteins.
32485989	2	12	gly	glycoprotein	264:275	arg1	This 34 kDa glycoprotein	This 34 kDa glycoprotein				Fterm		glycoprotein			This 34 kDa glycoprotein, termed VaaSP-VX, possesses five kDa N-linked carbohydrates.
32485989	2	57	gly	possesses	295:303	arg1	This 34 kDa glycoprotein AND five kDa N-linked carbohydrates	This 34 kDa glycoprotein			five kDa N-linked carbohydrates	Fterm		glycoprotein			This 34 kDa glycoprotein, termed VaaSP-VX, possesses five kDa N-linked carbohydrates.
37238633	6	47	gly	core-fucosylated	1039:1054	arg1	agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans				agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans						We observed an increase in agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans (padj = 1.00 × 10-4, 2.41 × 10-25, 1.51 × 10-21 and 3.38 × 10-30, respectively) and a decrease in digalactosylated, mono- and di-sialylated N-glycans (padj = 4.93 × 10-12, 7.61 × 10-9 and 1.09 × 10-28, respectively).
37238633	6	49	gly	asialylated	1023:1033	arg1	agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans				agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans						We observed an increase in agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans (padj = 1.00 × 10-4, 2.41 × 10-25, 1.51 × 10-21 and 3.38 × 10-30, respectively) and a decrease in digalactosylated, mono- and di-sialylated N-glycans (padj = 4.93 × 10-12, 7.61 × 10-9 and 1.09 × 10-28, respectively).
37238633	6	53	gly	di-sialylated	1192:1204	arg1	digalactosylated, mono- and di-sialylated N-glycans				digalactosylated, mono- and di-sialylated N-glycans						We observed an increase in agalactosylated, monogalctosylated, asialylated and core-fucosylated N-glycans (padj = 1.00 × 10-4, 2.41 × 10-25, 1.51 × 10-21 and 3.38 × 10-30, respectively) and a decrease in digalactosylated, mono- and di-sialylated N-glycans (padj = 4.93 × 10-12, 7.61 × 10-9 and 1.09 × 10-28, respectively).
36660462	3	24	gly	glycopeptide	506:517	arg2	intact glycopeptide			intact glycopeptide						glycopeptide	Here, tandem mass tag labeling combined with LC-MS/MS was employed to determine the global expression of all proteins and intact glycopeptide in placentas from three healthy pregnant women, three patients with early-onset severe PE, and three patients with late-onset severe PE.
35504880	3	29	gly	glycopeptide	454:465	arg2	a glycopeptide			a glycopeptide						glycopeptide	Through biophysical and cellular studies, including X-ray crystallography of C1GalT1 complexed to a glycopeptide, we report that C1GalT1 is an obligate GT-A fold dimer that follows a SN2 mechanism.
36585837	3	33	gly	attachment	324:333	arg2	asparagine AND glycans			asparagine	glycans					asparagine	Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-link glycosylation.
36585837	3	48	gly	attachment	277:286	arg2	proteins AND sugar moieties	proteins			sugar moieties	Fterm		proteins			Glycosylation is the attachment of sugar moieties to proteins, with attachment of glycans to asparagine indicated as N-link glycosylation.
37071468	2	14	gly	sialylated	241:250	arg1	the optimal Fc-glycan				the optimal Fc-glycan						The α-2,6 sialylated biantennary complex type glycan was identified as the optimal Fc-glycan with significant enhancement in antibody effector functions, including binding to different Fc receptors and ADCC.
37071468	2	14	gly	sialylated	241:250	arg1	The α-2,6 sialylated biantennary complex type glycan				The α-2,6 sialylated biantennary complex type glycan						The α-2,6 sialylated biantennary complex type glycan was identified as the optimal Fc-glycan with significant enhancement in antibody effector functions, including binding to different Fc receptors and ADCC.
31657490	8	4	gly	glycopeptide	1460:1471	arg2	glycopeptide analysis			glycopeptide analysis						glycopeptide	CONCLUSIONS The experimental results showed that GO@PDA@MIL-125-NH2 @Au@L-Cys exhibited excellent performance on glycopeptide analysis.
36881660	3	33	gly	glycans	478:484	arg1	the variable region			the variable region	the variable region		Site			region	In this technical note, we propose a method for facile characterization of glycans in the variable region of antibodies using sequential enzymatic digests with Endoglycosidase-S2 and Rapid™ Peptide-N-Glycosidase-F followed by labeling with a fluorescent dye carrying an NHS-carbamate moiety.
35960654	3	80	gly	glycopeptides	573:585	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Relatively large amounts of starting materials are needed for the enrichment, which makes the identification and quantification of intact glycopeptides from samples with limited quantity more challenging.
37129482	3	6	gly	glycoproteins	490:502	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycogenes, glycoproteins, lectins, pathways, and disease information related to glycans are accessible from GlyCosmos.
35366731	5	33	gly	N-glycosylation	537:551	arg2	only one N-glycosylation site			only one N-glycosylation site						site	RhGlu16B has only one N-glycosylation site in its Ala55-Gly64 loop.
35622127	0	91	gly	N-glycosylation	50:64	arg1	plasma proteins	plasma proteins				Fterm		proteins			Children at onset of type 1 diabetes show altered N-glycosylation of plasma proteins and IgG.
36385669	4	5	gly	contained	713:721	arg1	both isoforms AND high mannose glycans	both isoforms			high mannose glycans	Fterm		isoforms			Mass spectrometry data indicated that both isoforms contained high mannose glycans being mannose 6 the most abundant form.
37266972	5	22	gly	deglycosylated	722:735	arg1	deglycosylated proteins	deglycosylated proteins				Fterm		proteins			Here, we report on the generation of BY-2 cell lines producing deglycosylated proteins.
31657490	6	85	gly	glycopeptides	1138:1150	arg2	glycopeptides			glycopeptides						glycopeptides	RESULTS In the actual enrichment process, GO@PDA@MIL-125-NH2 @Au@L-Cys nanomaterials exhibited high selectivity (1:1000), outstanding sensitivity (0.5 fmol), and excellent repeatability for the enrichment of glycopeptides.
35077806	6	31	gly	found	966:970	arg2	vertebrate proteins AND various N- and O-glycan structures	vertebrate proteins			various N- and O-glycan structures	Fterm		proteins			Our analyses allowed the annotation of various N- and O-glycan structures commonly found in vertebrate proteins.
34611874	7	48	gly	terminus	1230:1237	arg1	both glycan reducing terminus fluorophore labeling				both glycan reducing terminus fluorophore labeling						In this chapter, we describe an analytical method for the UHPLC separation of plasma N-glycans which utilizes both glycan reducing terminus fluorophore labeling, to ensure stoichiometric analysis of relative glycan abundance, and online mass spectrometry for glycan identification.
35481895	8	19	part_of	PGF-CTERM	1423:1431	arg1	a predicted PGF-CTERM processing site	PGF		a predicted PGF-CTERM processing site		OGER	Site	PGF	P49763	site	While the S-layer protein has a predicted PGF-CTERM processing site, no evidence of a truncated and lipidated C-terminus, the expected product of processing by an archaeosortase, was found.
35481895	8	15	part_of	has	1407:1409	arg1	the S-layer protein AND a predicted PGF-CTERM processing site	the S-layer protein		a predicted PGF-CTERM processing site		Fterm	Site	protein		site	While the S-layer protein has a predicted PGF-CTERM processing site, no evidence of a truncated and lipidated C-terminus, the expected product of processing by an archaeosortase, was found.
32820167	4	23	gly	O-glycosites	497:508	arg2	the identified O-glycosites			the identified O-glycosites						O-glycosites	Many of the identified O-glycosites are conserved and are predicted to serve roles in proprotein processing, receptor interaction, biodistribution and biostability.
36007953	1	19	gly	N-glycosylated	214:227	arg1	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein				Fterm		protein			Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S protein) is highly N-glycosylated, and a "glycan shield" is formed to limit the access of other molecules; however, a small open area coincides with the interface to the host's receptor and also neutralising antibodies.
35077806	10	51	gly	glycoproteins	1441:1453	arg1	O- linked glycoproteins	O- linked glycoproteins				Fterm		glycoproteins			Finally, secreted N- and O- linked glycoproteins were predicted from the transcriptomic data, indicating that proteases and protease inhibitors are putative sources of the glycans described herein.
34712242	1	6	gly	glycan	299:304	arg1	the Fc region			the Fc region	the Fc region		Site			region	Rhesus macaques are a common non-human primate model used in the evaluation of human monoclonal antibodies, molecules whose effector functions depend on a conserved N-linked glycan in the Fc region.
34689907	8	67	gly	glycoprotein	1610:1621	arg1	a model glycoprotein	a model glycoprotein				Fterm		glycoprotein			The new approach was demonstrated to be applicable for the analysis of N-linked oligosaccharides released from a model glycoprotein (Human Immunoglobulin G) and applied to map N-glycans from human serum for congenital disorders of glycosylation (CDG) diagnosis.
36754227	3	14	gly	N-glycan	426:433	arg1	the luminal domain			the luminal domain	the luminal domain		Site			domain	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
36754227	3	42	gly	position	586:593	arg1	559			559						position 559	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
36754227	3	77	gly	position	438:445	arg1	the N-glycan			position 480	the N-glycan					position 480	Our computational data suggest that the N-glycan at position 480 (N480g) in the luminal domain of SV2C (LD-SV2C) indirectly enhanced the contacts of the neurotoxin surface with the second N-glycan at position 559 (N559g) by acting as a shield to prevent N559g to interact with residues of LD-SV2C.
34957216	1	65	gly	glycoprotein	308:319	arg1	its spike glycoprotein	its spike glycoprotein				Fterm		glycoprotein			Severe Acute respiratory syndrome coronavirus (SARS-CoV-1) attaches to the host cell surface to initiate the interaction between the receptor-binding domain (RBD) of its spike glycoprotein (S) and the human Angiotensin-converting enzyme (hACE2) receptor.
35943155	14	81	gly	fucosylated	2298:2308	arg1	These fucosylated glycans				These fucosylated glycans						These fucosylated glycans are involved in a number of mammalian physiological processes, including adhesion of pathogens and immune responses.
35017635	4	30	gly	fucosylated	801:811	arg1	O-glycan fucosylated structures				O-glycan fucosylated structures						These different glycan profiles, confirmed by glycomics, can be distinguished by the expression of O-glycan fucosylated structures, present only in epithelial cells and regulated by the expression of GALNT3.
36373229	1	58	gly	glycoproteins	144:156	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			O-Glycoproteases are an emerging class of enzymes that selectively digest glycoproteins at positions decorated with specific O-linked glycans.
31941975	7	32	gly	N-glycoproteins	1343:1357	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Finally, this model was applied to classify fucosylated N-glycoproteins from human plasma.
31941975	7	76	gly	fucosylated	1331:1341	arg1	fucosylated N-glycoproteins	fucosylated N-glycoproteins				Fterm		N-glycoproteins			Finally, this model was applied to classify fucosylated N-glycoproteins from human plasma.
35887202	5	55	gly	O-glycopeptide	731:744	arg2	MUC1 O-glycopeptide			MUC1 O-glycopeptide						O-glycopeptide	The aim of this study is to elucidate the mode of interaction between MY.1E12 and MUC1 O-glycopeptide by NMR, molecular dynamics (MD) and docking simulations.
35065968	1	64	gly	linked	264:269	arg2	an N-methylated threonine AND GlcNAc			an N-methylated threonine	GlcNAc					threonine	The type A glycan modification found in human pathogen Clostridioides difficile consists of a monosaccharide (GlcNAc) that is linked to an N-methylated threonine through a phosphodiester bond.
35065968	1	64	gly	linked	264:269	arg2	an N-methylated threonine AND a monosaccharide			an N-methylated threonine	a monosaccharide					threonine	The type A glycan modification found in human pathogen Clostridioides difficile consists of a monosaccharide (GlcNAc) that is linked to an N-methylated threonine through a phosphodiester bond.
36985724	1	7	gly	glycoproteins	219:231	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Protein N-glycosylation is a common post-translational modification that plays significant roles on the structure, property, and function of glycoproteins.
37270132	1	41	gly	composition	240:250	arg1	β-D-Glcp-			position	β-D-Glcp-					position	A low molecular weight polysaccharides of HEP-1, with molecular weights of 1.67 × 104 Da and composition of →6)-β-D-Glcp-(1→, →3)-β-D-Glcp-(1→, β-D-Glcp-(1→ and →3,6)-β-D-Glcp-(1→, was isolated and characterized from the fruiting body of Hericium erinaceus.
36435935	2	58	part_of	sites	308:312	arg1	secretory proteins	proteins		sites		Fterm	Site	proteins	281499	sites	It catalyzes the transfer of a pre-assembled glycan, GlcNAc2Man9Glc3, from a dolichyl-pyrophosphate donor to acceptor sites in secretory proteins in the lumen of the endoplasmic reticulum.
36233110	5	10	part_of	VEGFR-2	1101:1107	arg1	the VEGFR-2 domain	VEGFR-2		the VEGFR-2 domain		PUBTATOR	Site	VEGFR-2	3791	domain	On the other hand, noticeable variations between lots in the glycan types and sialylation levels on the two sites of the VEGFR-2 domain were observed when each of the five N-glycosylation sites were assessed using the glycopeptide-based method.
35304921	2	46	gly	integrin	342:349	arg1	the first comprehensive site-specific glycan map	integrin			the first comprehensive site-specific glycan map	Fterm		integrin			RESULTS Here, we have established the first comprehensive site-specific glycan map of α5 β1 integrin that was purified from a natural source, that is, rat liver.
32409323	2	85	gly	modified	345:352	arg3	Most proteins AND N-glycans	Most proteins			N-glycans	Fterm		proteins			Most proteins in the secretory pathway, such as matrix metalloproteinases, are modified with N-glycans and play important roles in the development of osteoarthritis (OA).
32409323	2	85	gly	modified	345:352	arg3	matrix metalloproteinases AND N-glycans	matrix metalloproteinases			N-glycans	Fterm		metalloproteinases			Most proteins in the secretory pathway, such as matrix metalloproteinases, are modified with N-glycans and play important roles in the development of osteoarthritis (OA).
34695439	2	58	part_of	contain	401:407	arg1	TSR AND O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs	TSR		O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs		Cterm	Site	TSR		motifs	B3GLCT mediates modification of proteins with thrombospondin type I repeats (TSR) that contain O-linked glucose β1-3 fucose and C-linked mannose glycosylation motifs.
36740532	5	10	gly	glycopeptide	841:852	arg2	glycopeptide analysis			glycopeptide analysis						glycopeptide	METHODS GPIbα ectodomain that was recombinantly expressed or that was purified from human platelets was analyzed by Western blot, mass spectrometry glycomics, and mass spectrometry glycopeptide analysis to define glycosites and the structures of the attached glycans.
36740532	5	4	gly	glycosites	873:882	arg1	the attached glycans			glycosites	the attached glycans					glycosites	METHODS GPIbα ectodomain that was recombinantly expressed or that was purified from human platelets was analyzed by Western blot, mass spectrometry glycomics, and mass spectrometry glycopeptide analysis to define glycosites and the structures of the attached glycans.
32356523	1	31	gly	glycoproteins	388:400	arg1	a cargo receptor	a cargo receptor				Fterm		receptor			The transmembrane intracellular lectin ER-Golgi intermediate compartment protein 53 (ERGIC-53) and the soluble EF-hand multiple coagulation factor deficiency protein 2 (MCFD2) form a complex that functions as a cargo receptor, trafficking various glycoproteins between the endoplasmic reticulum (ER) and the Golgi apparatus.
32356523	1	31	gly	glycoproteins	388:400	arg1	trafficking various glycoproteins	trafficking various glycoproteins				Fterm		glycoproteins			The transmembrane intracellular lectin ER-Golgi intermediate compartment protein 53 (ERGIC-53) and the soluble EF-hand multiple coagulation factor deficiency protein 2 (MCFD2) form a complex that functions as a cargo receptor, trafficking various glycoproteins between the endoplasmic reticulum (ER) and the Golgi apparatus.
37357420	1	4	gly	fucosylated	174:184	arg1	a fucosylated cell-surface glycan				a fucosylated cell-surface glycan						Sialyl Lewis X (sLex ) antigen is a fucosylated cell-surface glycan that is normally involved in cell-cell interactions.
37357420	1	4	gly	fucosylated	174:184	arg1	Sialyl Lewis X (sLex ) antigen				Sialyl Lewis X (sLex ) antigen						Sialyl Lewis X (sLex ) antigen is a fucosylated cell-surface glycan that is normally involved in cell-cell interactions.
34102146	3	44	gly	sialo-glycoprotein	627:644	arg1	the underlying sialo-glycoprotein	the underlying sialo-glycoprotein				Fterm		sialo-glycoprotein			To determine the underlying sialo-glycoprotein, various regions in adjacent tissue sections were subjected to microdigestion and manual glycoproteomic analysis.
37258966	3	26	gly	glycosylated	478:489	arg1	aberrantly glycosylated proteins	aberrantly glycosylated proteins				Fterm		proteins			They also play an integral role in the immune system, and aberrantly glycosylated proteins have wide ranging effects, including leading to diseases such as autoimmune conditions and cancer.
34726173	5	26	part_of	SDR	1328:1330	arg1	the long SDR region	SDR		the long SDR region		PUBTATOR	Site	SDR	8436	region	Together with biophysical and biochemical analyses, this structural work uncovered the novel mechanism by which SdgB and SdgA carry out the glycosyl-transfer process to the long SDR region in SDR proteins.
34726173	5	72	part_of	region	1332:1337	arg1	SDR proteins	SDR proteins		region		PUBTATOR	Site	SDR proteins	8436	region	Together with biophysical and biochemical analyses, this structural work uncovered the novel mechanism by which SdgB and SdgA carry out the glycosyl-transfer process to the long SDR region in SDR proteins.
36493594	3	27	part_of	IgG	769:771	arg1	three glycosylation sites	IgG		three glycosylation sites		Cterm	Site	IgG		sites	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	27	part_of	IgG	769:771	arg1	a single glycosylation site	IgG		a single glycosylation site		Cterm	Site	IgG		site	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	80	part_of	fetuin	719:724	arg1	three glycosylation sites	fetuin		three glycosylation sites		Fterm	Site	fetuin		sites	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	80	part_of	fetuin	719:724	arg1	a single glycosylation site	fetuin		a single glycosylation site		Fterm	Site	fetuin		site	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
35616904	4	7	gly	N-glycosites	708:719	arg2	1019 N-glycosites			1019 N-glycosites						N-glycosites	A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
35616904	4	67	gly	glycopeptides	602:614	arg2	9567 unique glycopeptides			9567 unique glycopeptides						glycopeptides	A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
35616904	4	62	gly	glycoproteins	730:742	arg1	620 glycoproteins	glycoproteins			773 N-linked glycan structures	Fterm		glycoproteins			A total of 9567 unique glycopeptides identified in human seminal plasma were composed of 773 N-linked glycan structures and 1019 N-glycosites from 620 glycoproteins.
34452239	4	1	part_of	Ang	955:957	arg1	the native Ang (1-7) peptide	Ang (1-7		the native Ang (1-7) peptide		OGER	Site	Ang (1-7	Q15389	peptide	We have synthesized the native Ang (1-7) peptide and the glycopeptide, PNA5, and have formulated them for targeted respiratory delivery as inhalable dry powders.
34452239	4	6	part_of	1-7	960:962	arg1	the native Ang (1-7) peptide	Ang (1-7		the native Ang (1-7) peptide		OGER	Site	Ang (1-7	Q15389	peptide	We have synthesized the native Ang (1-7) peptide and the glycopeptide, PNA5, and have formulated them for targeted respiratory delivery as inhalable dry powders.
35343965	4	6	gly	glycoproteins	519:531	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In pathogenic bacteria, glycoproteins have been involved in different stages of the infectious process, and glycan modifications can interfere with specific functions of glycoproteins.
35343965	4	85	gly	glycoproteins	665:677	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In pathogenic bacteria, glycoproteins have been involved in different stages of the infectious process, and glycan modifications can interfere with specific functions of glycoproteins.
36252735	2	39	gly	glycoproteins	232:244	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The glycans and glycoproteins are involved in cell-cell interactions that are of fundamental importance to the brain.
36606688	3	29	gly	contains	362:369	arg1	an extracellular superoxide dismutase AND a single N-glycan chain	an extracellular superoxide dismutase			a single N-glycan chain	Fterm		dismutase			SOD3 is an extracellular superoxide dismutase and contains a single N-glycan chain.
34735575	3	24	part_of	spike	831:835	arg1	the spike N-terminal domain	spike N-terminal		the spike N-terminal domain		PUBTATOR	Site	spike N-terminal	43740568	domain	The Alpha variant derived from the D614G lineage is distinguished from others by having deletion mutations located right within an immunogenic supersite of the spike N-terminal domain (NTD) that make it refractory to most neutralizing antibodies directed against this domain.
34735575	3	76	part_of	N-terminal	837:846	arg1	the spike N-terminal domain	spike N-terminal		the spike N-terminal domain		PUBTATOR	Site	spike N-terminal	43740568	domain	The Alpha variant derived from the D614G lineage is distinguished from others by having deletion mutations located right within an immunogenic supersite of the spike N-terminal domain (NTD) that make it refractory to most neutralizing antibodies directed against this domain.
36055406	9	24	part_of	GPI-APs	1720:1726	arg1	C-terminal sequences	APs		C-terminal sequences		OGER	Site	APs	P07288	sequences	Taking these findings together, this study revealed N- and C-terminal sequences of GPI-APs determine the selective ER entry route, which in turn regulates subsequent maturation processes of GPI-APs.
33073321	5	48	gly	glycopeptides	1090:1102	arg2	glycopeptides			glycopeptides						glycopeptides	More excitingly, glycopeptides in urine of healthy people and patients with kidney cancer were successfully enriched and identified by the combined liquid chromatography-mass spectrometry/mass spectrometry technology (LC-MS/MS).
36437732	5	82	gly	released	799:806	arg2	serum glycoproteins AND N-glycans	serum glycoproteins			N-glycans	Fterm		glycoproteins			N-glycans were enzymatically released from serum glycoproteins, labeled via permethylation, and analyzed by MALDI-TOF mass spectrometry.
36437732	5	89	gly	glycoproteins	819:831	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			N-glycans were enzymatically released from serum glycoproteins, labeled via permethylation, and analyzed by MALDI-TOF mass spectrometry.
35495330	6	17	part_of	peptides	1329:1336	arg1	N-terminal cysteine	peptides		N-terminal cysteine						cysteine	Thus, mono- and dimannosylated peptides with N-terminal cysteine facilitated site-specific interactions with HA peptides, mimicking a naturally found N-linked glycosylation site on the HA head domain.
36827096	4	62	part_of	containing	616:625	arg1	a glycoprotein AND two N-glycosylation sites	a glycoprotein		two N-glycosylation sites		Fterm	Site	glycoprotein		sites	GYL is a glycoprotein containing two N-glycosylation sites per subunit.
37162352	8	5	gly	O-glycosylated	1186:1199	arg1	Six proteins	Six proteins				Fterm		proteins			Six proteins associated with gliding motility or the T9SS were demonstrated to be O-glycosylated.
36233110	2	6	part_of	IgG	606:608	arg1	the human IgG Fc region	IgG		the human IgG Fc region		Cterm	Site	IgG		region	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
36233110	2	30	part_of	VEGFR-1	531:537	arg1	the VEGFR-1 region	VEGFR-1		the VEGFR-1 region		PUBTATOR	Site	VEGFR-1	2321	region	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
36233110	2	68	part_of	VEGFR-2	564:570	arg1	the VEGFR-2 region	VEGFR-2		the VEGFR-2 region		PUBTATOR	Site	VEGFR-2	3791	region	Glycopeptide-based nano-LC MSMS mapping of tryptic-digested samples of each aflibercept lot provided site-specific information about glycan microheterogeneity on each of the five N-glycosylation sites (two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region).
35887202	6	51	gly	O-glycosylation	921:935	arg2	the O-glycosylation site			the O-glycosylation site						site	NMR titration using MUC1 O-glycopeptides suggests that the epitope is located within the O-linked glycan and near the O-glycosylation site.
35887202	6	53	gly	O-glycopeptides	828:842	arg2	MUC1 O-glycopeptides			MUC1 O-glycopeptides						O-glycopeptides	NMR titration using MUC1 O-glycopeptides suggests that the epitope is located within the O-linked glycan and near the O-glycosylation site.
36089065	5	81	gly	glycoproteins	896:908	arg1	These glycoproteins	These glycoproteins				Fterm		glycoproteins			These glycoproteins were then sequentially modified in vitro from high mannose to hybrid and on to biantennary, core-fucosylated, complex structures by a panel of N-glycosylation enzymes, and each reaction time course was quantified by LC-MS/MS.
36809652	2	105	gly	subunit	313:319	arg1	The O-glycan structures	subunit			The O-glycan structures	Fterm		subunit			The O-glycan structures of apo(a) subunit of Lp(a) serve as strong ligands of galectin-1, an O-glycan binding pro-angiogenic lectin abundantly expressed in placental vascular tissues.
33493676	7	99	gly	glycopeptides	1204:1216	arg2	AGP glycopeptides			AGP glycopeptides						glycopeptides	After trypsinization, AGP glycopeptides are purified using a hydrophilic interaction chromatography-based solid-phase extraction and analyzed by reversed-phase-liquid chromatography-electrospray ionization-MS.
36329887	6	18	gly	Asn<sup>52</sup>	1184:1199	arg1	Asn<sup>52</sup> glycans			Asn	Asn<sup>52</sup> glycans					Asn	As the most active FSH21 preparations possessed more rapidly migrating α-subunit bands in Western blots, we hypothesized that Asn<sup>52</sup> glycans in these preparations were small enough to enable greater FSH21 receptor occupancy in the putative FSHR trimer model.
34778211	9	45	gly	glycosites	1516:1525	arg2	928 identified glycosites			928 identified glycosites						glycosites	Moreover, 28.62% in 442 identified glycoproteins and 26.69% in 928 identified glycosites were significantly changed in the FUT8KO CHO compared to wild-type CHO cells.
34778211	9	93	gly	glycoproteins	1473:1485	arg1	442 identified glycoproteins	442 identified glycoproteins				Fterm		glycoproteins			Moreover, 28.62% in 442 identified glycoproteins and 26.69% in 928 identified glycosites were significantly changed in the FUT8KO CHO compared to wild-type CHO cells.
36380917	6	14	gly	glycopeptides/glycoproteins	1335:1361	arg1	N-glycan			glycopeptides/glycoproteins	N-glycan					glycopeptides/glycoproteins	The information learned would facilitate future design of therapeutic glycopeptides/glycoproteins with N-glycan to achieve enhanced pharmacological properties.
33710874	9	14	gly	motifs	1679:1684	arg1	a model peptide			a model peptide						peptide	The plug-and-play trifunctional platform was exemplified by attaching clinically relevant motifs: biotin, fluorescein, a polyethylene glycol chain, and a model peptide.
34626388	3	56	gly	galactosamine	563:575	arg1	heparin	heparin			galactosamine	Fterm		heparin			Testing galactosamine in heparin has been added to the USP monograph after contaminated heparin event, but the general monosaccharide composition analysis has not been developed for GAG quality control purposes.
37398203	8	36	gly	-glycan	1416:1422	arg1	the Fab region			the Fab region	the Fab region		Site			region	Incorporation of enzymatic de- N -glycosylation into the peptide mapping routine allows the N -glycan in the Fab region of the antibody to be localized to the framework 3 region of the V H domain.
35219398	4	29	gly	glycosylation	675:687	arg1	dimer formation	dimer formation				Fterm		dimer			GFCC'C″ was identified as the dominant CCM1 solution dimer, but ambiguity regarding the effect of glycosylation on dimer formation calls its physiological relevance into question.
32545589	3	12	part_of	receptors	549:557	arg1	phosphorylation sites	receptors		phosphorylation sites		Fterm	Site	receptors		sites	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
32545589	3	12	part_of	receptors	549:557	arg1	putative N-linked glycosylation motifs	receptors		putative N-linked glycosylation motifs		Fterm	Site	receptors		motifs	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
32545589	3	12	part_of	receptors	549:557	arg1	seven membrane spanning domains	receptors		seven membrane spanning domains		Fterm	Site	receptors		domains	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
32545589	3	12	part_of	receptors	549:557	arg1	threonine	receptors		serine and threonine residues		Fterm	AminoAcid	receptors		serine and threonine residues	The architecture of HdhGnRH-R gene exhibited key features of G protein-coupled receptors (GPCRs), including seven membrane spanning domains, putative N-linked glycosylation motifs, and phosphorylation sites of serine and threonine residues.
32187935	12	60	part_of	protein	1368:1374	arg1	the second hypervariable region	G protein		the second hypervariable region		OGER	Site	G protein		region	Phylogenetic tree analysis of the second hypervariable region (HVR2) of the G protein classified the HRSV-A specimens into ON1 (n=62) and NA1 (n=2) genotypes while all HRSV-B specimens belonged to BA genotype (n=53).
36912209	8	32	gly	position	1479:1486	arg1	the carbohydrate			position	the carbohydrate					position	We highlight examples of acetylesterases that are highly specific to one substrate and to the position of the acetyl group on the glycosyl residue of the carbohydrate, while other members that have more broad substrate specificity.
35889277	2	21	part_of	glycoproteins	377:389	arg1	composition	structure of glycoproteins		composition		Fterm	Site	structure of glycoproteins		position	Hence, the development of analytical methods able to characterize the composition and structure of glycoproteins is crucial.
36252443	10	79	gly	glycoproteins	1674:1686	arg1	native glycoproteins	native glycoproteins				Fterm		glycoproteins			EndoBI-1 released a broader array of glycan structures compared to PNGase F from native glycoproteins.
34778211	7	108	gly	glycopeptides	1082:1094	arg2	The glycopeptides			The glycopeptides						glycopeptides	The glycopeptides were enriched by hydrophilic chromatography and fractionated 25 fractions by bRPLC followed by analysis using high-resolution liquid chromatography mass spectrometry (LC-MS).
37316505	5	42	gly	sialylated	815:824	arg1	sialylated mucin-type O-glycan with core 1-				sialylated mucin-type O-glycan with core 1-						The result indicates that T153, S157, S159, and T168 are modified by sialylated mucin-type O-glycan with core 1- and core 2-based structures.
34053187	2	97	part_of	Disintegrin	313:323	arg1	ThrombSpondin Motif type 1 motif	Disintegrin		ThrombSpondin Motif type 1 motif		Fterm	Site	Disintegrin		motif	The multimeric size and function of VWF are normally regulated through A Disintegrin And Metalloprotease with ThrombSpondin Motif type 1 motif, member 13 (ADAMTS-13)--mediated proteolysis.
36122299	3	30	gly	glycopeptides	373:385	arg2	glycopeptides			glycopeptides						glycopeptides	Despite the great progress, characterization of structural isomers of glycans and glycopeptides remains challenging.
33493676	1	55	gly	glycoprotein	198:209	arg1	Alpha-1-acid glycoprotein	glycoprotein				Fterm		glycoprotein			Alpha-1-acid glycoprotein (AGP) is an acute phase glycoprotein in blood, which is primarily synthetized in the liver and whose biological role is not completely understood.
33493676	1	69	gly	glycoprotein	161:172	arg1	an acute phase glycoprotein	glycoprotein				Fterm		glycoprotein			Alpha-1-acid glycoprotein (AGP) is an acute phase glycoprotein in blood, which is primarily synthetized in the liver and whose biological role is not completely understood.
37292769	7	59	gly	glycopeptide	1165:1176	arg2	synthetic stable isotope-labelled glycopeptide standards			synthetic stable isotope-labelled glycopeptide standards						glycopeptide	Here, we investigated fragmentation specificity using synthetic stable isotope-labelled glycopeptide standards.
34878920	2	78	gly	glycosylation	313:325	arg2	the 13 predicted N-linked glycosylation sites			the 13 predicted N-linked glycosylation sites						sites	In this study, we found that, among the 13 predicted N-linked glycosylation sites (NGSs) in gp85 of Tibetan chicken strain TBC-J6, N17, and N193/N191 are pivotal for virus replication.
36903897	3	41	gly	glycoproteins	635:647	arg1	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins				Fterm		glycoproteins			Here, our work with Hyp-O-polysaccharides isolated from (Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins overexpressed in transgenic Arabidopsis suspension culture is consistent with the common structural features of AGPs isolated from tobacco.
36903897	3	59	gly	isolated	560:567	arg2	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins AND Hyp-O-polysaccharides	(Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins			Hyp-O-polysaccharides	Fterm		glycoproteins			Here, our work with Hyp-O-polysaccharides isolated from (Ser-Hyp)32-EGFP (enhanced green fluorescent protein) fusion glycoproteins overexpressed in transgenic Arabidopsis suspension culture is consistent with the common structural features of AGPs isolated from tobacco.
34778211	11	38	gly	core-fucosylated	1842:1857	arg1	core-fucosylated glycans				core-fucosylated glycans						Furthermore, a decrease in fucosylation content was observed in FUT8KO cells, in which core-fucosylated glycans almost disappeared as an effect of FUT8 gene knockout.
35247653	5	32	gly	glycoprotein	877:888	arg1	the intact glycoprotein	the intact glycoprotein				Fterm		glycoprotein			At the intact glycoprotein level, NeuroEPO plus glycoforms were separated by capillary zone electrophoresis with ultraviolet detection (CE-UV), optimizing the composition and pH of the separation electrolyte.
35854001	5	8	part_of	lectin	648:653	arg1	the lectin domain	lectin		the lectin domain		Fterm	Site	lectin	269181	domain	Here, we identify the lectin domain in mouse GnT-IVa's C-terminal region.
35854001	5	30	part_of	domain	655:660	arg1	mouse GnT-IVa's C-terminal region	domain		mouse GnT-IVa's C-terminal region						region	Here, we identify the lectin domain in mouse GnT-IVa's C-terminal region.
33316265	1	9	gly	proteins	201:208	arg1	O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			AIM We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats.
33316265	1	9	gly	proteins	201:208	arg1	O-linked N-acetylglucosamine	proteins			O-linked N-acetylglucosamine	Fterm		proteins			AIM We determined the role played by O-linked N-acetylglucosamine (O-GlcNAc) of proteins in systemic arteries during late pregnancy in normotensive and hypertensive rats.
34184536	8	15	part_of	Fab	1605:1607	arg1	the primary antibody's Fab fragment	Fab		the primary antibody's Fab fragment		PUBTATOR	Site	Fab	2187	fragment	The simulations revealed that the flexible hinge between Fab and Fc segments effectively randomized the direction of the secondary antibody, while the restricted binding orientation of the primary antibody's Fab fragment accounted for most of the systematic offset between the reporter and α-tubulin.
32273875	0	65	gly	Removal	0:6	arg3	Mannose-Ending Glycan AND Asn2118			Asn2118	Mannose-Ending Glycan					Asn2118	Removal of Mannose-Ending Glycan at Asn2118 Abrogates FVIII Presentation by Human Monocyte-Derived Dendritic Cells.
35848837	8	55	gly	Asn-glycans	1167:1177	arg1	Asn-glycans and free glycans			Asn	Asn-glycans and free glycans					Asn	The resulted Asn-glycans and free glycans were then purified using porous graphitic carbon cartridges, permethylated, and analyzed by mass spectrometry.
36737995	2	88	gly	glycosylation	343:355	arg1	free milk oligosaccharides				free milk oligosaccharides						However glycosylation of milk components other than free milk oligosaccharides are underinvestigated.
36585837	9	0	part_of	sites	1311:1315	arg1	220 glycoproteins	glycoproteins		sites		Fterm	Site	glycoproteins		sites	Shotgun proteome analysis identified 545 N-linked and glutamine, Q, -linked, glycosylation (P>0.75 for deamidation) sites on 220 glycoproteins in sow milk.
36192063	6	10	gly	N-glycoproteins	1380:1394	arg1	254 intact N-glycoproteins	254 intact N-glycoproteins				Fterm		N-glycoproteins			In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36192063	6	100	gly	N-glycopeptides	1256:1270	arg2	6384 intact N-glycopeptides			6384 intact N-glycopeptides						N-glycopeptides	In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36192063	6	96	gly	N-glycopeptides	1348:1362	arg1	254 intact N-glycoproteins	N-glycoproteins		N-glycopeptides		Fterm		N-glycoproteins		N-glycopeptides	In this study, 6384 intact N-glycopeptides without sialylation were identified and 521 differentially expressed intact N-glycopeptides from 254 intact N-glycoproteins were quantified.
36136114	8	19	gly	glycosylation	1303:1315	arg1	a fusion protein	a fusion protein				Fterm		protein			To overcome this drawback, we used nanoHILIC-MS to characterize the complex glycosylation of UTI-Fc, a fusion protein that greatly increases the half-life of ulinastatin.
32555531	2	37	gly	glycoprotein	483:494	arg1	glomalin-related soil protein	glomalin-related soil protein				Fterm		protein			In a field experiment, the temporal and spatial dynamics of AM fungi, glomalin-related soil protein (GRSP) - which is described as a N-linked glycoprotein and the putative gene product of AM fungi, SOC, and soil aggregates were investigated in halophyte Kosteletzkya virginica rhizosphere soil of coastal saline areas of North Jiangsu, China.
32555531	2	37	gly	glycoprotein	483:494	arg1	a N-linked glycoprotein	a N-linked glycoprotein				Fterm		glycoprotein			In a field experiment, the temporal and spatial dynamics of AM fungi, glomalin-related soil protein (GRSP) - which is described as a N-linked glycoprotein and the putative gene product of AM fungi, SOC, and soil aggregates were investigated in halophyte Kosteletzkya virginica rhizosphere soil of coastal saline areas of North Jiangsu, China.
33206215	2	27	gly	proteoglycans	557:569	arg1	lysines			lysines	lysines		AminoAcid			prolines and lysines	The extracellular matrix (ECM) consists of (1) fibrous proteins, post-translationally modified (PTM) via N- and O-linked glycosylation, as well as hydroxylation on prolines and lysines, and (2) glycosaminoglycan-decorated proteoglycans.
33206215	2	27	gly	proteoglycans	557:569	arg1	prolines			prolines	prolines		AminoAcid			prolines and lysines	The extracellular matrix (ECM) consists of (1) fibrous proteins, post-translationally modified (PTM) via N- and O-linked glycosylation, as well as hydroxylation on prolines and lysines, and (2) glycosaminoglycan-decorated proteoglycans.
36089065	6	44	gly	sites	1233:1237	arg1	the same protein	protein			sites	Fterm		protein			Substantial differences in rates of in vitro enzymatic modification were observed between glycan sites on the same protein, and differences in modification rates varied depending on the glycoenzyme being evaluated.
35995381	8	10	gly	glycopeptide	1267:1278	arg2	the glycopeptide level			the glycopeptide level						glycopeptide	In addition, sulfated glycoform structures were detected at the glycopeptide level in glycosylation sites containing complex and hybrid type glycans.
35995381	8	77	gly	glycosylation	1289:1301	arg2	glycosylation sites			glycosylation sites						sites	In addition, sulfated glycoform structures were detected at the glycopeptide level in glycosylation sites containing complex and hybrid type glycans.
35995381	8	68	gly	containing	1309:1318	arg1	glycosylation sites AND complex and hybrid type glycans			glycosylation sites	complex and hybrid type glycans					sites	In addition, sulfated glycoform structures were detected at the glycopeptide level in glycosylation sites containing complex and hybrid type glycans.
35393560	6	23	gly	glycoprotein	961:972	arg1	an impactful glycoprotein class	an impactful glycoprotein class				Fterm		glycoprotein			In this study, we quantitatively evaluate more than 30 enzymes for glycoengineering immobilized immunoglobulin G, an impactful glycoprotein class in the pharmaceutical field.
36096000	0	40	gly	Glycosylation	0:12	arg1	egg white protein	egg white protein				Fterm		protein			Glycosylation of egg white protein with maltodextrin in the dry state: Changes in structural and gel properties.
36252443	4	18	gly	deglycosylated	588:601	arg1	the deglycosylated protein	the deglycosylated protein				Fterm		protein			Only by testing both the released glycans and the deglycosylated protein in their native (i.e., non-denatured) form, can the individual contribution to the biological activity of glycoproteins be elucidated.
36252443	4	20	gly	glycoproteins	717:729	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Only by testing both the released glycans and the deglycosylated protein in their native (i.e., non-denatured) form, can the individual contribution to the biological activity of glycoproteins be elucidated.
37354227	3	11	gly	N-glycosylation	522:536	arg2	22 potential sites			22 potential sites						sites	The SARS-CoV-2 spike protein (S) is a prime example, having 22 potential sites of N-glycosylation per protein protomer, as predicted from the primary sequence.
36373229	4	4	gly	O-glycopeptides	752:766	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	One challenge of defining O-glycoprotease specificity restraints is the need to characterize O-glycopeptides with site-specific analysis of O-glycosites.
36373229	4	14	gly	O-glycosites	799:810	arg2	O-glycosites			O-glycosites						O-glycosites	One challenge of defining O-glycoprotease specificity restraints is the need to characterize O-glycopeptides with site-specific analysis of O-glycosites.
36628504	8	74	part_of	SHP	1491:1493	arg1	composition	SHP		composition		OGER	Site	SHP	Q15466	position	CONCLUSION Overall, ammonium oxalate and sodium citrate significantly influenced the composition and properties of the SHP.
36797772	6	51	part_of	FAS1	1350:1353	arg1	FAS1 domains	FAS1		FAS1 domains		OGER	Site	FAS1	P25445	domains	We identified FAS1 domains as essential for FLA function, differentiating FLA11/FLA12, with roles in SCW development, from FLA3, specific to flowers and involved in pollen development.
37079606	5	7	gly	afucosylated	1114:1125	arg1	IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan				IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan						RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	53	gly	fucosylated	1081:1091	arg1	IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan				IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan						RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	81	gly	trisialylated	1167:1179	arg1	IgA trisialylated triantennary (A3G3S3) glycan				IgA trisialylated triantennary (A3G3S3) glycan						RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	100	gly	protein	987:993	arg1	core fucosylation	protein			core fucosylation	Fterm		protein			RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
37079606	5	100	gly	protein	987:993	arg1	sialylation	protein			sialylation	Fterm		protein			RESULTS Fasting insulin exhibited significant associations to numerous glycan traits, including plasma protein galactosylation, sialylation, branching, core fucosylation and bisection, to IgG core fucosylated, bisected (FA2B) and afucosylated disialylated (A2G2S2) glycan and to IgA trisialylated triantennary (A3G3S3) glycan (padj range: 4.37x10-05-4.94x10-02).
35211008	14	131	gly	α-1,3-mannosyl-glycoprotein	2613:2639	arg1	α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase A	α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase A				Fterm		α-1,3-mannosyl-glycoprotein	12044		Conclusion: Our results show that stachytine hydrochloride inhibits the synthesis of α-1,6-fucosylation on the N-terminal sugar chain by reducing α-1,6-fucosyltransferase (FUT8) and α-1,3-mannosyl-glycoprotein 4-β-N-acetylglucosaminyltransferase A (MGAT4a), upregulating the N-glycosylation level on β1 adrenergic receptors, and maintaining cAMP/PKA signal pathway activation.
37186866	4	48	gly	domains	961:967	arg1	O-mannosylation			domains	O-mannosylation					domains	We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knockout in cells results in receptor maturation defects and abnormal growth of 3D cell models.
37186866	4	63	gly	O-mannosylation	938:952	arg1	IPT domains			IPT domains						domains	We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knockout in cells results in receptor maturation defects and abnormal growth of 3D cell models.
33946304	5	38	gly	glycoprotein	941:952	arg1	glycoprotein N-linked oligosaccharide processing				glycoprotein N-linked oligosaccharide processing						The mechanism resides in the inhibitory activity toward α-glucosidases that are involved in the early stages of glycoprotein N-linked oligosaccharide processing in the endoplasmic reticulum, leading to a marked decrease of the viral Spike protein.
34853076	3	2	part_of	contains	348:355	arg1	Native C5a AND a large N-linked glycosylation site	Native C5a		a large N-linked glycosylation site		PUBTATOR	Site	Native C5a	728	site	Native C5a contains a large N-linked glycosylation site at Asn64, which accounts for up to 25% of its m.w. To date, the vast majority of published studies examining C5a are performed using Escherichia coli-generated recombinant C5a, which is readily available from numerous commercial suppliers, but lacks this glycosylation moiety.
32357974	7	62	part_of	HLA-bound	1536:1544	arg1	deamidated HLA-bound peptides	HLA		deamidated HLA-bound peptides		OGER	Site	HLA		peptides	Chemical inhibition of Peptide:N-Glycanase (PNGase), the endoglycosidase responsible for the removal of glycans from misfolded and retrotranslocated glycoproteins, greatly reduced presentation of this subset of deamidated HLA-bound peptides.
36399934	8	80	gly	aglycone	1252:1259	arg1	Thr193			Thr193						Thr193	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	81	gly	glycone	1231:1237	arg1	binding sites			binding sites						sites	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	linker residues			linker residues						residues	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	Phe333			Leu192, Ile251, and Phe333						Leu192, Ile251, and Phe333	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	Leu192			Leu192, Ile251, and Phe333						Leu192, Ile251, and Phe333	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
36399934	8	85	gly	residues	1314:1321	arg1	Leu192			Leu192, Ile251, and Phe333						Leu192, Ile251, and Phe333	The high specific activity of the recombinant β-glucosidase Glu1B was correlated with the presence of specific residues in the glycone (Gln455) and aglycone (Thr193 and Hys252) binding sites, along with linker residues (Leu192, Ile251, and Phe333) between residues of these two sites.
33006605	0	13	gly	glycoprotein	59:70	arg1	glycoprotein stability	glycoprotein stability				Fterm		glycoprotein			Systemic effects of missense mutations on SARS-CoV-2 spike glycoprotein stability and receptor-binding affinity.
33552045	10	27	gly	glycosylation	1695:1707	arg1	viral proteins	viral proteins				Fterm		proteins			Overall, this perspective is aimed to support future research on T cells recognizing glycosylated epitopes in order to expand our understanding on how glycosylation of viral proteins could alter host T cell immunity against viral infections.
33552045	10	76	gly	glycosylated	1629:1640	arg1	glycosylated epitopes			glycosylated epitopes						epitopes	Overall, this perspective is aimed to support future research on T cells recognizing glycosylated epitopes in order to expand our understanding on how glycosylation of viral proteins could alter host T cell immunity against viral infections.
36399685	6	6	gly	glycoprotein	1092:1103	arg1	an arrested glycoprotein	an arrested glycoprotein				Fterm		glycoprotein			Moreover, the observation that encoding mRNAs remained stably attached to ribosomes provides evidence of a genotype-glycophenotype link between an arrested glycoprotein and its RNA message.
36289103	7	99	gly	sialylated	977:986	arg1	sialylated N-glycans				sialylated N-glycans						The levels of sialylated N-glycans showed considerable cell line-dependent differences ranging from 24 to 35%.
32357974	10	2	gly	glycoprotein	2061:2072	arg1	glycoprotein antigens	glycoprotein antigens				Fterm		glycoprotein			The information herein will help train deamidation prediction models for HLA-peptide repertoires and aid in the design of novel T cell therapeutic targets derived from glycoprotein antigens.
32306345	3	30	gly	glycoproteins	391:403	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The microneme protein 4 (MIC4) has the property to recognize galactosides residues linked to glycoproteins on the host cell surface.
37146275	6	34	gly	glycosylation	1422:1434	arg2	the glycosylation site			the glycosylation site						site	A total of 677 quantifiable intact N-glycopeptides were identified from the serum of patients with cervical cancer, and the glycosylation site and glycan structure were analyzed in depth, indicating that this novel material can show a broad practical application in cervical cancer diagnosis.
37146275	6	35	gly	N-glycopeptides	1333:1347	arg2	677 quantifiable intact N-glycopeptides			677 quantifiable intact N-glycopeptides						N-glycopeptides	A total of 677 quantifiable intact N-glycopeptides were identified from the serum of patients with cervical cancer, and the glycosylation site and glycan structure were analyzed in depth, indicating that this novel material can show a broad practical application in cervical cancer diagnosis.
36039611	1	23	part_of	BTC	235:237	arg1	Novel β-cyclodextrin (β-CD)@functionalized graphene (G)/Cu-1,3,5-benzenetricarboxylic acid (BTC) composites	BTC		Novel β-cyclodextrin (β-CD)@functionalized graphene (G)/Cu-1,3,5-benzenetricarboxylic acid (BTC) composites		OGER	Site	BTC	P35070	composites	Novel β-cyclodextrin (β-CD)@functionalized graphene (G)/Cu-1,3,5-benzenetricarboxylic acid (BTC) composites were in situ prepared using β-CD functionalized graphene and Cu-BTC, and a new electrochemical sensor for sensitive detection of chloramphenicol (CAP) was developed based on the composites.
37292769	3	44	gly	glycoproteins	534:546	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The ability to distinguish between these complex glycan structures poses a significant challenge, hindering our ability to accurately measure and understand the role of glycoproteins in biological systems.
35960654	8	27	gly	glycopeptides	1614:1626	arg2	glycopeptides			glycopeptides						glycopeptides	Moreover, weighted gene coexpression network analysis (WGCNA) showed nine modules of glycopeptides, two of which were associated with the AD phenotype.
36053804	3	49	gly	enzyme	547:552	arg1	high-mannose glycan moieties	enzyme			high-mannose glycan moieties	Fterm		enzyme			Previously, RNAi-mediated silencing of Mannosidase-Ia (ManIa), a key enzyme in the transition from high-mannose glycan moieties to paucimannose N-glycans, was shown to disrupt the transition from larva to pupa and the metamorphosis into adult beetles.
36053804	3	69	gly	moieties	597:604	arg1	a key enzyme	enzyme			moieties	Fterm		enzyme			Previously, RNAi-mediated silencing of Mannosidase-Ia (ManIa), a key enzyme in the transition from high-mannose glycan moieties to paucimannose N-glycans, was shown to disrupt the transition from larva to pupa and the metamorphosis into adult beetles.
32719124	1	41	part_of	CD8+	134:137	arg1	CD8+ T cell-activating peptides	CD8		CD8+ T cell-activating peptides		PUBTATOR	Site	CD8	925	peptides	The source proteins from which CD8+ T cell-activating peptides are derived remain enigmatic.
32187935	16	0	gly	glycosylation	1916:1928	arg2	glycosylation sites			glycosylation sites						sites	A greater diversification of amino acid substitutions, and some deletion and insertion of glycosylation sites embodied the polymorphism of G protein as main protective antigen.
35193013	2	94	gly	glycoproteins	457:469	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The acidic sugars are attached to the end of glycoproteins, glycolipids or gangliosides.
37294165	7	44	gly	glycoprotein	1359:1370	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Because the two peptides used for this synthesis were prepared by a fast-flow SPPS, the total synthetic time of glycoprotein was considerably shortened.
34274480	12	44	gly	glycosylation	1553:1565	arg2	three possible N-linked glycosylation sites			three possible N-linked glycosylation sites						sites	The amino terminal ends of both the CBLN1 and CBLN3 proteins contain three possible N-linked glycosylation sites.
35304921	14	13	gly	glycosylation	1973:1985	arg2	the specific sites			the specific sites						sites	Yet, detailed knowledge of its glycosylation, notably the specific sites of glycosylation, is lacking.
37347675	6	64	part_of	elastin-like	1289:1300	arg1	genetically engineered elastin-like polypeptides	elastin		genetically engineered elastin-like polypeptides		OGER	Site	elastin	P15502	polypeptides	The HT-LbL method was also used to investigate the deposition of PEMs with a small library of genetically engineered elastin-like polypeptides (ELPs) with different levels of ionizable and hydrophobic amino acid residues.
37121976	4	8	gly	N-glycosylation	788:802	arg1	LRG1	structure of LRG1		sites		OGER		structure of LRG1	P02750	sites	Here, we determined the crystal structure of LRG1, identifying the horseshoe-like solenoid structure of LRG1 and its four N-glycosylation sites.
32796070	2	64	part_of	sequences	309:317	arg1	the viral Env glycoprotein gp120	gp120		sequences		PUBTATOR		gp120	3700		HIV-1 susceptibility to SERINC5 is determined by sequences in the viral Env glycoprotein gp120, and the antiviral effect of SERINC5 is counteracted by the viral accessory protein Nef.
34379775	5	22	gly	fucosylated	1078:1088	arg1	fucosylated glycans				fucosylated glycans						We identified 124 O-glycans, from which 51 were the major glycans with core 2 and fucosylated glycans as the most common structures.
36422041	2	85	gly	modified	493:500	arg3	glycoproteins AND both N-linked and O-linked glycans	glycoproteins			both N-linked and O-linked glycans	Fterm		glycoproteins			Like other fungal pathogens, the Sporothrix cell wall is composed of structural polysaccharides and glycoproteins that are covalently modified with both N-linked and O-linked glycans.
36422041	2	92	gly	glycoproteins	459:471	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Like other fungal pathogens, the Sporothrix cell wall is composed of structural polysaccharides and glycoproteins that are covalently modified with both N-linked and O-linked glycans.
36581037	8	7	part_of	MBP-NPs	1271:1277	arg1	the phenolic composition	MBP		the phenolic composition		OGER	Site	MBP	P02686	position	Compared with that of unencapsulated MBP, the significant variation was occurred in the phenolic composition of MBP-NPs, indicating that MBP-NPs could prevent the degradation and transformation of phenolic compounds.
33176830	7	32	gly	N-glycosylation	1340:1354	arg2	non-canonical N-glycosylation motifs			non-canonical N-glycosylation motifs						motifs	It is also the first study to report the functional importance of non-canonical N-glycosylation motifs in an ion channel.
37238633	5	83	part_of	IgG	933:935	arg1	IgG N-glycome composition	IgG		IgG N-glycome composition		Cterm	Site	IgG		position	Exercise intervention induced significant changes in IgG N-glycome composition.
33073321	4	43	gly	glycopeptides	1036:1048	arg2	glycopeptides			glycopeptides						glycopeptides	Benefitting from the merits of Zr-MOF and glucose-6-phosphate, the as-designed composite exhibits good selectivity (the mass ratio of HRP digests to BSA digests was up to1:200) and low limit of detection (0.1 fmol μL-1) towards the recognition of glycopeptides from standard samples.
34939126	1	42	gly	glycoprotein	145:156	arg1	new glycoprotein variants	new glycoprotein variants				Fterm		glycoprotein			Glycoengineering ultimately allows control over glycosylation patterns to generate new glycoprotein variants with desired properties.
35092134	9	49	gly	domain	1404:1409	arg1	the N-glycans			domain	the N-glycans					domain	The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling.
35092134	9	54	gly	domain	1303:1308	arg1	the N-glycans			domain	the N-glycans					domain	The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling.
35092134	9	69	gly	region	1377:1382	arg1	the N-glycans				the N-glycans						The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling.
33244686	1	31	gly	glycosylation	306:318	arg2	multiple glycosylation site(s)			multiple glycosylation site(s)						site	Many biotherapeutics such as monoclonal antibodies (mAb) and Fc-domain fusion proteins contain heterogeneous glycan contents at one or multiple glycosylation site(s).
33244686	1	74	gly	contain	249:255	arg1	Fc-domain fusion proteins AND heterogeneous glycan contents	Fc-domain fusion proteins			heterogeneous glycan contents	Fterm		proteins			Many biotherapeutics such as monoclonal antibodies (mAb) and Fc-domain fusion proteins contain heterogeneous glycan contents at one or multiple glycosylation site(s).
34611874	4	7	gly	glycosylated	633:644	arg1	the proteins	the proteins				Fterm		proteins			Many of the proteins that constitute plasma are glycosylated with both N- and O-type glycans.
32187935	13	79	gly	glycosylation	1588:1600	arg2	glycosylation sites			glycosylation sites						sites	The HVR2 of the G protein varied in using stop condon, amino acid substitutions, glycosylation sites.
37186866	2	30	part_of	immunoglobulin	318:331	arg1	extracellular immunoglobulin, plexin, transcription factor (IPT) domains	immunoglobulin		extracellular immunoglobulin, plexin, transcription factor (IPT) domains		Fterm	Site	immunoglobulin		domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
37186866	2	54	part_of	IPT	731:733	arg1	these IPT domains	IPT		these IPT domains		OGER	Site	IPT	Q9H3H1	domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
37186866	2	64	part_of	found	377:381	arg2	macrophage-stimulating protein receptor AND extracellular immunoglobulin, plexin, transcription factor (IPT) domains	macrophage-stimulating protein receptor		extracellular immunoglobulin, plexin, transcription factor (IPT) domains		OGER	Site	macrophage-stimulating protein receptor	Q04912	domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
37186866	2	64	part_of	found	377:381	arg2	the hepatocyte growth factor receptor AND extracellular immunoglobulin, plexin, transcription factor (IPT) domains	the hepatocyte growth factor receptor		extracellular immunoglobulin, plexin, transcription factor (IPT) domains		OGER	Site	hepatocyte growth factor receptor	P08581	domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
37186866	2	64	part_of	found	377:381	arg2	plexin receptors AND extracellular immunoglobulin, plexin, transcription factor (IPT) domains	plexin receptors		extracellular immunoglobulin, plexin, transcription factor (IPT) domains		Fterm	Site	receptors		domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
37186866	2	64	part_of	found	377:381	arg1	cMET AND extracellular immunoglobulin, plexin, transcription factor (IPT) domains	cMET		extracellular immunoglobulin, plexin, transcription factor (IPT) domains		Cterm	Site	cMET	P08581	domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
37186866	2	64	part_of	found	377:381	arg1	RON AND extracellular immunoglobulin, plexin, transcription factor (IPT) domains	RON		extracellular immunoglobulin, plexin, transcription factor (IPT) domains		OGER	Site	RON	Q04912	domains	We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin, plexin, transcription factor (IPT) domains found in the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors, and further demonstrated that two known protein O-mannosylation systems orchestrated by the POMT1/2 and transmembrane and tetratricopeptide repeat-containing proteins 1-4 gene families were not required for glycosylation of these IPT domains.
35022995	2	19	gly	residues	405:412	arg1	173-210			173-210						residues 173-210	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
35022995	2	40	gly	N-glycosylation	337:351	arg2	position 143			position 143						position 143	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
35022995	2	40	gly	N-glycosylation	337:351	arg2	one potential N-glycosylation site			one potential N-glycosylation site						site	DynAP is a single-pass transmembrane protein with a carboxy-terminal region (amino acids 135-210) exposed to the outside of the cell possessing one potential N-glycosylation site (position 143) and a distal C-terminal region (residues 173-210) harboring a Thr/Ser-rich (T/S) cluster that may be O-glycosylated.
36838558	2	18	gly	glycans	323:329	arg1	Gc proteins	proteins			glycans	Fterm		proteins			O-linked glycans in Gc proteins from human plasma mainly consist of trisaccharides.
35597280	1	65	part_of	containing	195:204	arg1	small protein motifs AND six conserved cysteines	small protein motifs		six conserved cysteines						cysteines	Thrombospondin type-1 repeats (TSRs) are small protein motifs containing six conserved cysteines forming three disulfide bonds that can be modified with an O-linked fucose.
35247773	3	27	part_of	protein	464:470	arg1	protein composition	protein		protein composition		Fterm	Site	protein		position	The SDC was confirmed by analyzing the changes in protein composition and infrared absorption bands and showed loosened tertiary conformation, reduced surface hydrophobicity, decreased Z-average hydrodynamic diameter (Dh) and zeta potential, and improved emulsifying properties compared to the native SPI.
36892535	5	27	gly	glycosylated	764:775	arg1	up to 6 differently glycosylated MUC1 peptides			up to 6 differently glycosylated MUC1 peptides						peptides	Cell adhesion experiments with MCF-7 cancer cells on surfaces carrying up to 6 differently glycosylated MUC1 peptides demonstrated that different glycans significantly impact adhesion.
36536394	3	35	gly	β-1,4-glycosylation	569:587	arg1	glucose				glucose						Bottom-up production of cello-oligosaccharides (COS) involves multistep iterative β-1,4-glycosylation of glucose from sucrose catalyzed by sucrose phosphorylase from Bifidobacterium adeloscentis (BaScP), cellobiose phosphorylase from Cellulomonas uda (CuCbP) and cellodextrin phosphorylase from Clostridium cellulosi (CcCdP).
35495330	2	70	gly	dimannosylated	417:430	arg1	Chemically synthesized dimannosylated HA peptides			Chemically synthesized dimannosylated HA peptides						peptides	Chemically synthesized dimannosylated HA peptides bound domain-swapped and dimeric CV-N with either four disulfide-bonds (Cys-Cys), or three Cys-Cys bonds and an intact fold of the high-affinity binding site at an equilibrium dissociation constant K D of 10 μM.
37341462	2	23	gly	N-glycopeptide	496:509	arg2	N-glycopeptide identification			N-glycopeptide identification						N-glycopeptide	The Y-ion pattern, a series of Y-ions with known mass gaps derived from the penta-saccharide core structure of N-linked glycosylation, is exploited to facilitate N-glycopeptide identification from their spectra.
36652139	1	69	gly	glycoprotein	154:165	arg1	a glycoprotein hormone	a glycoprotein hormone				Fterm		glycoprotein			Equine chorionic gonadotropin (eCG) is a glycoprotein hormone widely used in timed artificial ovulation (TAI) and superovulation protocols to improve the reproductive performance in livestock.
35647713	5	53	gly	glycoprotein	975:986	arg1	a viral glycoprotein	glycoprotein			N-glycans	Fterm		glycoprotein			It involves the attachment of an immune potentiator to CMP-Neu5AcN3 by Cu(I)-catalyzed azide-alkyne 1,3-cycloaddition followed by sialyltransferase-mediated transfer to N-glycans of a viral glycoprotein.
33442744	2	76	part_of	asparagine	453:462	arg1	a nascent protein	protein		asparagine		Fterm	AminoAcid	protein		asparagine	In the central step of this reaction, a carbohydrate moiety is transferred from a lipid-linked donor to the side-chain of a consensus asparagine in a nascent protein as it is synthesized at the ribosome.
36830744	6	17	gly	sialylated	1118:1127	arg1	sialylated N-glycan structures				sialylated N-glycan structures						The most notable differences were observed in women, where the presence of sialylated N-glycan structures was negatively associated with CAD.
36493594	3	0	gly	glycosylation	788:800	arg2	a single glycosylation site			a single glycosylation site						site	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	46	gly	glycosylation	738:750	arg2	three glycosylation sites			three glycosylation sites						sites	METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	75	gly	glycoproteins	697:709	arg1	two model mammalian glycoproteins	two model mammalian glycoproteins				Fterm		glycoproteins			METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36493594	3	75	gly	glycoproteins	697:709	arg1	bovine fetuin	bovine fetuin				Fterm		fetuin			METHODS N-glycans are released by peptide-N-glycosidase F (PF) or A (PA) from two model mammalian glycoproteins, bovine fetuin (with three glycosylation sites) and human IgG (with a single glycosylation site), and labeled with a fluorescent tag [2-aminobenzamide (AB) or procainamide (ProA)].
36056620	2	56	gly	glycosylation	252:264	arg1	viral envelope proteins	viral envelope proteins				Fterm		proteins			N-linked glycosylation of viral envelope proteins is a key mechanism for such evasion.
35532124	1	12	part_of	factor	252:257	arg1	the A1 domain	von Willebrand factor		the A1 domain		PUBTATOR	Site	von Willebrand factor	7450	domain	Hemostasis in the arterial circulation is mediated by binding of the A1 domain of the ultralong protein von Willebrand factor (VWF) to GPIbα on platelets to form a platelet plug.
35532124	1	40	part_of	A1	202:203	arg1	the A1 domain	A1		the A1 domain		PUBTATOR	Site	A1	28881	domain	Hemostasis in the arterial circulation is mediated by binding of the A1 domain of the ultralong protein von Willebrand factor (VWF) to GPIbα on platelets to form a platelet plug.
36409896	3	1	gly	glycoproteins	550:562	arg1	off-target glycoproteins	off-target glycoproteins				Fterm		glycoproteins			Here, we describe a method to identify and characterize a class of nanobodies that can distinguish glycoforms without reactivity to off-target glycoproteins or glycans.
36233110	7	53	part_of	containing	1533:1542	arg1	fusion proteins AND multiple N-glycosylation sites	fusion proteins		multiple N-glycosylation sites		Fterm	Site	proteins		sites	These results imply that analyses of the glycosylation profiles of fusion proteins containing multiple N-glycosylation sites, such as aflibercept, being done as a part of quality control for the therapeutics manufacturing process or for biosimilar development, can be done with a more applicable outcome by assessing each site separately.
36940546	1	13	part_of	-Rhap-	230:235	arg1	containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues	Rhap-(1		containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues		OGER	Site	Rhap-(1		1 → residues	In this study, a novel 28.6 kDa acidic polysaccharide (HTP-1), containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues as the backbone, analogous to pectin, was isolated from mature Hawk tea leaves.
36940546	1	26	part_of	1 → and → 3,6	237:249	arg1	containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues	3,6)-Galp		containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues		OGER	Site	3,6)-Galp	Q9UBC7	1 → residues	In this study, a novel 28.6 kDa acidic polysaccharide (HTP-1), containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues as the backbone, analogous to pectin, was isolated from mature Hawk tea leaves.
36940546	1	32	part_of	-Galp-	251:256	arg1	containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues	3,6)-Galp		containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues		OGER	Site	3,6)-Galp	Q9UBC7	1 → residues	In this study, a novel 28.6 kDa acidic polysaccharide (HTP-1), containing → 4)-GalpA-(1→, →2)-Rhap-(1 → and → 3,6)-Galp-(1 → residues as the backbone, analogous to pectin, was isolated from mature Hawk tea leaves.
35887202	0	30	part_of	MUC1	39:42	arg1	MUC1 Glycopeptide	MUC1		MUC1 Glycopeptide		PUBTATOR	Site	MUC1	4582	Glycopeptide	O-Glycan-Dependent Interaction between MUC1 Glycopeptide and MY.1E12 Antibody by NMR, Molecular Dynamics and Docking Simulations.
34662212	2	17	part_of	TonB	520:523	arg1	the TonB C-terminal domain	TonB		the TonB C-terminal domain		Cterm	Site	TonB		domain	The inner membrane TonB/ExbB/ExbD complex harvests energy from the proton motive force (Δp), and the TonB C-terminal domain interacts with and transduces energy to outer membrane TonB-dependent transporters (TBDTs).
34102146	1	9	gly	glycoproteins	193:205	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Here, we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of matrix-assisted laser desorption ionization (MALDI) N-glycan mass spectrometry imaging (MSI) and spatially resolved glycoproteomics.
37235553	7	15	gly	N-glycans	1219:1227	arg1	this database	database			N-glycans	Fterm		database			Many N-glycans in this database are not found in current N-glycan mass spectrum libraries.
36211377	0	61	part_of	IgG	65:67	arg1	IgG glycome composition	IgG		IgG glycome composition		Cterm	Site	IgG		position	Effects of low-calorie and different weight-maintenance diets on IgG glycome composition.
36675089	6	20	part_of	PPL	706:708	arg1	the composite	PPL		the composite		OGER	Site	PPL	O60437	composite	This study shows that PPL in the composite not only improves the ZnO dispersivity in PVA as a crosslinker, but also enhances the water barrier of PVA.
37189353	4	7	gly	N-glycosylation	587:601	arg1	the plasma proteins	the plasma proteins				Fterm		proteins			To assess the changes in the N-glycosylation of the plasma proteins and IgG in atrial fibrillation, we analyzed the N-glycosylation of 172 patients with atrial fibrillation, before and six months after a pulmonary vein isolation procedure, with 54 cardiovascularly healthy controls.
32357974	8	6	part_of	containing	1626:1635	arg1	peptides AND a consensus NX(S/T) motif	peptides		a consensus NX(S/T) motif						motif	Importantly, there was no impact of PNGase inhibition on peptides not containing a consensus NX(S/T) motif.
37121976	4	39	part_of	LRG1	770:773	arg1	its four N-glycosylation sites	structure of LRG1		its four N-glycosylation sites		OGER	Site	structure of LRG1	P02750	sites	Here, we determined the crystal structure of LRG1, identifying the horseshoe-like solenoid structure of LRG1 and its four N-glycosylation sites.
36007953	3	45	gly	N-glycosylation	545:559	arg2	N-glycosylation sites			N-glycosylation sites						sites	In contrast, N-glycosylation sites are relatively invariant, and some are essential for infection.
35943155	6	19	gly	fucosylation	1212:1223	arg1	N-glycans				N-glycans						In addition, Fuc1584 also hydrolyzed fucosyl-α-1,6-N-acetylglucosamine (6FN), a component of the core fucosylation of N-glycans.
36959283	5	73	gly	fetuin	857:862	arg1	quantitative N-glycan profiling	fetuin			quantitative N-glycan profiling	Fterm		fetuin			Qualitative and quantitative N-glycan profiling of purified human serum IgG, bovine serum fetuin, bovine pancreas ribonuclease B, blood-derived extracellular vesicle isolates, and total plasma results in the detection of >250, >400, >150, >310, and >520 N-glycans, respectively, using injected amounts equivalent to <25 ng of model protein and nL-levels of plasma-derived samples.
36031042	2	20	part_of	contains	382:389	arg1	glycoprotein C3 AND two N-glycosylation sites	glycoprotein C3		two N-glycosylation sites		Cterm	Site	C3		sites	The most abundant complement component, glycoprotein C3, contains two N-glycosylation sites occupied exclusively by this type of glycans.
37186866	3	52	gly	glycosylates	851:862	arg1	IPT domains			IPT domains						domains	Here, we report that the TMEM260 gene encodes an ER-located protein O-mannosyltransferase that selectively glycosylates IPT domains.
34695439	4	6	gly	glycosylation	657:669	arg1	proteins	proteins				Fterm		proteins			We aimed to start understanding the role of B3GLCT in AMD by evaluating its effect on glycosylation and secretion of proteins from retinal pigment epithelium (RPE) cells.
32839225	0	41	gly	Asparagine	15:24	arg1	Sialylation			Asparagine 612	Sialylation					Asparagine 612	Sialylation of Asparagine 612 Inhibits Aconitase Activity during Mouse Sperm Capacitation; a Possible Mechanism for the Switch from Oxidative Phosphorylation to Glycolysis.
32839225	3	50	gly	glycoproteins	599:611	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Regardless of its importance, to date, no single study has ever identified nor quantified which glycoproteins bearing terminal sialic acid (Sia) are altered during capacitation.
32296440	5	37	gly	glycosylated	895:906	arg1	the three glycosylated asparagines			the three glycosylated asparagines						asparagines	The role of SR-F1 N-linked glycans was also tested by mutating each of the three glycosylated asparagines.
36136114	2	63	gly	glycoprotein	260:271	arg1	glycoprotein therapeutic pharmacokinetics	glycoprotein therapeutic pharmacokinetics				Fterm		glycoprotein			Importantly, glycosylation modulates glycoprotein therapeutic pharmacokinetics, pharmacodynamics, and effector functions.
35104505	4	76	part_of	GnT-V	706:710	arg1	the luminal region	GnT-V		the luminal region		PUBTATOR	Site	GnT-V	4249	region	In this study, we focused on an uncharacterized domain at the N-terminal side of the luminal region (N domain) of GnT-V, which was previously identified in a crystal structure, and aimed to reveal its role in GnT-V action.
35104505	4	76	part_of	GnT-V	706:710	arg1	N domain	GnT-V		N domain		PUBTATOR	Site	GnT-V	4249	domain	In this study, we focused on an uncharacterized domain at the N-terminal side of the luminal region (N domain) of GnT-V, which was previously identified in a crystal structure, and aimed to reveal its role in GnT-V action.
36534501	2	19	part_of	MMP-14	384:389	arg1	the catalytic domain	MMP-14		the catalytic domain		PUBTATOR	Site	MMP-14	4323	domain	Lumican, a member of the SLRPs family, and its derived peptides were shown to possess antitumor activity by interacting directly with the catalytic domain of MMP-14 leading to the inhibition of its activity.
36437732	9	63	gly	fucosylated	1371:1381	arg1	biosynthetically related biantennary core fucosylated N-glycans				biosynthetically related biantennary core fucosylated N-glycans						The three glycans were biosynthetically related biantennary core fucosylated N-glycans that only varied by the degree of galactosylation (G0F, G1F, and G2F; G: galactose, F: fucose).
36437732	9	63	gly	fucosylated	1371:1381	arg1	The three glycans				The three glycans						The three glycans were biosynthetically related biantennary core fucosylated N-glycans that only varied by the degree of galactosylation (G0F, G1F, and G2F; G: galactose, F: fucose).
37341462	1	62	gly	O-glycopeptide	290:303	arg2	O-glycopeptide spectra			O-glycopeptide spectra						O-glycopeptide	Different from N-linked glycosylation, the core structures of mucin type O-glycans are much more diverse, and the sensitive interpretation of O-glycopeptide spectra remains a challenge.
37146275	5	20	gly	glycopeptides	1156:1168	arg2	glycopeptides			glycopeptides						glycopeptides	This microscopically highly hydrophilic material shows excellent enrichment ability for glycopeptides, such as extremely low detection limits (0.01 fmol μL-1), remarkable selectivity (1:8000), and size exclusion effects (1:8000).
32356523	2	12	gly	glycans	588:594	arg1	cargo glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
32356523	2	29	gly	glycoproteins	605:617	arg1	cargo glycoproteins	cargo glycoproteins				Fterm		glycoproteins			It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
32356523	2	37	gly	glycoproteins	675:687	arg1	cargo glycoproteins	cargo glycoproteins				Fterm		glycoproteins			It has been demonstrated that the carbohydrate-recognition domain (CRD) of ERGIC-53 (ERGIC-53CRD) interacts with N-linked glycans on cargo glycoproteins, whereas MCFD2 recognizes polypeptide segments of cargo glycoproteins.
36478308	13	56	gly	glycopeptide	1931:1942	arg2	our glycopeptide libraries			our glycopeptide libraries						glycopeptide	The use of our glycopeptide libraries greatly expedited the generation of site-specific glycan occupancy data for a large glycoprotein.
36478308	13	77	gly	glycoprotein	2038:2049	arg1	a large glycoprotein	a large glycoprotein				Fterm		glycoprotein			The use of our glycopeptide libraries greatly expedited the generation of site-specific glycan occupancy data for a large glycoprotein.
33801653	2	7	gly	cycling	370:376	arg1	substrate proteins	proteins			cycling	Fterm		proteins			Two enzymes are responsible for O-GlcNAc cycling on substrate proteins: O-GlcNAc transferase (OGT) catalyzes the addition while O-GlcNAcase (OGA) helps the removal of GlcNAc.
33552045	4	23	gly	glycosylated	597:608	arg1	glycosylated epitopes			glycosylated epitopes						epitopes	Although there is some evidence for T cell responses to glycosylated epitopes (glyco-epitopes) during viral infections in the literature, this aspect has been largely neglected for HIV.
32935560	5	4	gly	presence	885:892	arg2	N332 AND an N-linked glycan motif			N332	an N-linked glycan motif					N332	By analyzing glycosylation patterns in 82 subtype C viruses, we found that the presence of an N-linked glycan motif at position N413 and its absence at N332 were the most important predictors of neutralization resistance.
32935560	5	64	gly	position	925:932	arg1	an N-linked glycan motif			position N413	an N-linked glycan motif					position N413	By analyzing glycosylation patterns in 82 subtype C viruses, we found that the presence of an N-linked glycan motif at position N413 and its absence at N332 were the most important predictors of neutralization resistance.
36493594	9	4	gly	glycoprotein	1780:1791	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Thus, PF-ProA or PA-ProA allows for more effective identification and quantification of N-glycans than PF-AB in glycoprotein, particularly bovine fetuin.
36329887	3	51	part_of	FSH	603:605	arg1	more FSH binding sites	FSH		more FSH binding sites		OGER	Site	FSH		sites	Due to its ability to bind more rapidly to the FSH receptor and occupy more FSH binding sites than FSH24, hypo-glycosylated FSH21 exhibits greater biological activity.
33657401	1	46	gly	attached	136:143	arg2	proteins AND O-linked β-N-acetyl glucosamine	proteins			O-linked β-N-acetyl glucosamine	Fterm		proteins			O-linked β-N-acetyl glucosamine (O-GlcNAc) is attached to proteins under glucose-replete conditions; this posttranslational modification results in molecular and physiological changes that affect cell fate.
33657401	1	46	gly	attached	136:143	arg2	proteins AND O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			O-linked β-N-acetyl glucosamine (O-GlcNAc) is attached to proteins under glucose-replete conditions; this posttranslational modification results in molecular and physiological changes that affect cell fate.