doc_id sent_index relation_id relation trigger trigger_offset arg_num arg_base_np arg_protein arg_domain arg_site arg_sugar PSource SiteSource NProtein NID SiteName sent_text 31907319 3 50 gly presence 487:494 arg1 the proteins AND host glycans the proteins host glycans Fterm proteins The presence of host glycans on the proteins of microbial origin may prevent a strong protective immune response either through hindering access to key epitopes by lymphocytes or through altering immune responses by binding to immunoregulatory glycan-binding receptors on immune cells. 33933451 3 11 gly N-glycosylation 385:399 arg1 PMP22 trafficking PMP22 trafficking PUBTATOR PMP22 Q01453 Here we characterized the role of N-glycosylation in limiting PMP22 trafficking. 34188486 3 3 gly unglycosylated 383:396 arg1 recombinant CenA recombinant CenA Cterm CenA (rCenA However, it seems not crucial for functional activity and secretion since the unglycosylated counterpart, recombinant CenA (rCenA), is both bioactive and secretable in Escherichia coli. 32518939 0 12 gly modified 45:52 arg1 The single EGF-like domain AND O-Glucose PAMR1 domain O-Glucose PUBTATOR PAMR1 210622 domain The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 32518939 0 12 gly modified 45:52 arg3 The single EGF-like domain AND O-Fucose PAMR1 domain O-Fucose PUBTATOR PAMR1 210622 domain The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 32518939 0 12 gly modified 45:52 arg3 The single EGF-like domain AND O-GlcNAc PAMR1 domain O-GlcNAc PUBTATOR PAMR1 210622 domain The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 33292056 2 53 gly glycoprotein 460:471 arg1 The homotrimeric transmembrane spike (S) glycoprotein The homotrimeric transmembrane spike (S) glycoprotein Fterm glycoprotein The homotrimeric transmembrane spike (S) glycoprotein of coronaviruses which facilitates virus entry into the host cells is covered with N-linked glycans having oligomannose and complex sugars. 34677013 15 130 part_of O-GlcNAc 2357:2364 arg1 two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides O-GlcNAc two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides Cterm Site O-GlcNAc peptides Furthermore, two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides were used to assess whether MSH destroyed the O-GlcNAc modification at the same time. 34677013 15 144 part_of N3-O-GlcNAc 2367:2377 arg1 two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides O-GlcNAc two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides Cterm Site O-GlcNAc peptides Furthermore, two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides were used to assess whether MSH destroyed the O-GlcNAc modification at the same time. 32120884 5 1 gly glycoproteins 938:950 arg1 the glycoproteins the glycoproteins Fterm glycoproteins Some of the glycoproteins are predicted to have an additional mucin-like domain located prior to the transmembrane anchor, whereas others are predicted to have a stem consisting of two alpha-helices. 34155345 4 17 gly modification 506:517 arg1 PTC PTC modification OGER PTC P00740 Here we found that the upregulated O-Linked N-Acetylglucosamine Transferase (OGT) expression and O-GlcNAcylation (O-GlcNAc) modification in papillary thyroid cancer (PTC) were essential in tumor growth and metastasis. 33806920 2 43 gly glycosylation 430:442 arg1 the protein the protein Fterm protein Interestingly, after a prolonged incubation (>8 h) several proteoforms were visualized by Western blot, corresponding to different states of glycosylation of the protein. 32315666 10 11 part_of NACP + DMAHDM 1805:1817 arg1 The novel NACP + DMAHDM nanocomposite NACP The novel NACP + DMAHDM nanocomposite OGER Site NACP P37840 nanocomposite CONCLUSIONS The novel NACP + DMAHDM nanocomposite had strong antibacterial effects and Ca and P ion release. 32157140 6 63 part_of Gpr176 838:843 arg1 the N-terminal region Gpr176 the N-terminal region PUBTATOR Site Gpr176 Q14439 region Using a heterologous expression system, we show that N-glycosylation occurs at four conserved asparagine residues in the N-terminal region of Gpr176. 33167279 0 58 part_of sequence 48:55 arg1 three recognition sites sequence three recognition sites sites A lable-free SPR biosensor based on one peptide sequence with three recognition sites for O-GlcNAc transferase detection. 34622651 3 37 gly glycopeptides 418:430 arg2 intact N-linked glycopeptides intact N-linked glycopeptides glycopeptides Therefore, it is essential to elucidate protein modifications of glycosylation by quantitatively profiling intact N-linked glycopeptides. 32958677 2 31 gly glycoproteins 383:395 arg1 misfolded asparagine (N)-linked glycoproteins misfolded asparagine (N)-linked glycoproteins Fterm glycoproteins In the secretory pathway, misfolded asparagine (N)-linked glycoproteins are selectively sorted for endoplasmic reticulum-associated degradation (ERAD) in response to the catalytic removal of terminal alpha-linked mannose units. 33436086 7 10 gly glycosylation 1247:1259 arg1 this site site site H5N6 virus without glycosylation at this site triggered the host immune response mechanism to antagonize the viral infection, making viral pathogenicity milder and favoring virus spread. 34227365 15 64 gly N-glycoproteins 2764:2778 arg1 1016 N-glycoproteins 1016 N-glycoproteins Fterm N-glycoproteins A total of 1016 N-glycoproteins and 2192 N-glycopeptides were identified in the mid-morning urine samples of the 40 healthy volunteers. 34227365 15 98 gly N-glycopeptides 2789:2803 arg2 2192 N-glycopeptides 2192 N-glycopeptides N-glycopeptides A total of 1016 N-glycoproteins and 2192 N-glycopeptides were identified in the mid-morning urine samples of the 40 healthy volunteers. 32892942 9 96 gly N-glycopeptides 1538:1552 arg2 N-glycopeptides N-glycopeptides N-glycopeptides To show the potential of our material in solving real biological issues, N-glycopeptides in the serum from hepatocelluar carcinoma (HCC) patient and health control were enriched and quantified. 33419227 6 20 gly glycosylated 852:863 arg1 all regions all regions regions Interestingly, all regions that likely fold rapidly or were surrounded by lysines were not glycosylated even though an N-glycosylation sequon was present. 33419227 6 36 gly N-glycosylation 880:894 arg2 an N-glycosylation sequon an N-glycosylation sequon sequon Interestingly, all regions that likely fold rapidly or were surrounded by lysines were not glycosylated even though an N-glycosylation sequon was present. 33270417 7 10 gly nonglycosylated 992:1006 arg1 All the nonglycosylated peptides All the nonglycosylated peptides peptides All the nonglycosylated peptides have different half-lives ranging from one to 20 days, for the corresponding glycosylated peptides; all the results showed that the deamidation reaction was significantly reduced by the introduction of N-linked glycosylation. 33270417 7 49 gly glycosylated 1094:1105 arg1 the corresponding glycosylated peptides the corresponding glycosylated peptides peptides All the nonglycosylated peptides have different half-lives ranging from one to 20 days, for the corresponding glycosylated peptides; all the results showed that the deamidation reaction was significantly reduced by the introduction of N-linked glycosylation. 33997889 9 50 part_of ComP 1369:1372 arg1 the ComP sequon ComP the ComP sequon OGER Site ComP P49747 sequon We also demonstrate that the placement of the ComP sequon on the carrier protein is critical for stability and subsequent glycosylation. 33436086 4 84 gly glycosylation 727:739 arg1 site 158 site site Here we report that N-linked glycosylation at site 158 facilitated the assembly of virus-like particles and enhanced virus replication in A549, MDCK, and chicken embryonic fibroblast (CEF) cells. 33361116 2 42 part_of IgG-Fc 231:236 arg1 the IgG-Fc tail IgG the IgG-Fc tail Cterm Site IgG tail A highly conserved N-linked glycan within the IgG-Fc tail, which is essential for IgG function, shows variable composition in humans. 33536395 8 44 gly glycoprotein 997:1008 arg1 the secreted glycoprotein the secreted glycoprotein Fterm glycoprotein Although disruption of the gmn2+ gene is not lethal, the secreted glycoprotein showed a significant glycosylation defect with destabilization of the glycosyltransferase responsible for N-glycan elongation. 32598381 4 8 part_of NA 644:645 arg1 (NA Gly+) NA (NA Gly+) Cterm Site NA Gly+ To assess the effect this novel N-linked glycan has on virus fitness and antibody binding, recombinant viruses with (NA Gly+) or without (NA Gly-) the 245 NA glycan were created. 33242079 9 89 gly α2,6-sialylated 1462:1476 arg1 these α2,6-sialylated O-glycans these α2,6-sialylated O-glycans The majority of these α2,6-sialylated O-glycans were shown to be disialyl-T antigen and sialyl-(6)T antigen by mass spectrometry analysis. 32501708 7 18 part_of mucin 856:860 arg1 representative mucin glycopeptides mucin representative mucin glycopeptides PUBTATOR Site mucin 100508689 glycopeptides Here, we apply it to representative mucin glycopeptides with O-linked glycans: three GalNAc localization variants, a pair with α/β GalNAc anomers, and another with GalNAc/GlcNAc isomers. 34747275 3 61 part_of BT-NPs 394:399 arg1 The optimum composition BT-NPs The optimum composition OGER Site BT-NPs P0C0P6 position The optimum composition of BT-NPs was selected based on the point prediction method given by the Box Behnken design software. 34562451 3 50 gly glycosylation 528:540 arg1 TMPRSS13 TMPRSS13 PUBTATOR TMPRSS13 84000 In this study, we examined the role of N-linked glycosylation in the proteolytic activity, autoactivation, and cellular localization of TMPRSS13. 34478712 5 13 part_of BSF 984:986 arg1 BSF and PCF parasites BSF BSF and PCF parasites Cterm Site BSF parasites The absence of TbGT10 impaired the elaboration of N-glycans and GPI anchor side chains in BSF and PCF parasites, respectively. 33144078 5 39 gly glycoproteins 1273:1285 arg1 newly synthesized glycoproteins newly synthesized glycoproteins Fterm glycoproteins Functionally, the D1241E polymorphism led to a reduced level of FVIII in the Golgi apparatus because of its reduced association with malectin, which interacts with newly synthesized glycoproteins in the ER for FVIII folding and trafficking, leading to degradation of the minor allele of FVIII D1241E in the cytosol. 32958677 5 77 gly site 1140:1143 arg1 the Man1b1 luminal domain site the Man1b1 luminal domain site As expected, the conventional catalytic system requires an intact active site in the Man1b1 luminal domain. 34693225 4 57 gly present 580:586 arg1 the 63-residue peptide AND α-GalNAc the 63-residue peptide α-GalNAc peptide Electron transfer dissociation and higher-energy collisional dissociation tandem mass spectrometry were used to localize three α-N-acetylgalactosaminyl residues (α-GalNAc) present on the 63-residue peptide. 34925382 6 22 part_of HLA 1044:1046 arg1 detected O-GlcNAcylated HLA peptides HLA detected O-GlcNAcylated HLA peptides OGER Site HLA peptides Remarkably, the overlap in detected O-GlcNAcylated HLA peptides as well as their source proteins is strikingly high. 34030043 4 22 gly N-glycosylation 517:531 arg1 N101 N101 N101 N-glycosylation on N101 of TIGIT and, to less extent, on N32, play potent roles in PVR binding. 33206527 6 130 gly CES1 1134:1137 arg1 the N79 glycan CES1 the N79 glycan PUBTATOR CES1 1066 This antiproliferative function of the N79 glycan of CES1 was further supported by quantitative real-time polymerase chain reaction, flow cytometry, and an apoptosis protein array assay. 33436086 2 22 gly position 448:455 arg1 a key determinant position 158 a key determinant position 158 We have shown earlier that lack of glycosylation at position 158 of the hemagglutinin (HA) glycoprotein due to the T160A mutation is a key determinant of the dual receptor binding property of clade 2.3.4.4 H5NX subtypes. 33436086 2 62 gly glycoprotein 487:498 arg1 the hemagglutinin (HA) glycoprotein the hemagglutinin (HA) glycoprotein Fterm glycoprotein We have shown earlier that lack of glycosylation at position 158 of the hemagglutinin (HA) glycoprotein due to the T160A mutation is a key determinant of the dual receptor binding property of clade 2.3.4.4 H5NX subtypes. 33436086 2 80 gly glycosylation 431:443 arg1 position 158 position 158 position 158 We have shown earlier that lack of glycosylation at position 158 of the hemagglutinin (HA) glycoprotein due to the T160A mutation is a key determinant of the dual receptor binding property of clade 2.3.4.4 H5NX subtypes. 32222585 8 39 gly afucosylated 1315:1326 arg1 afucosylated IgG1 afucosylated IgG1 OGER IgG1 P01857 More specifically, the N162 glycan positively influenced the affinity (15-fold) for afucosylated IgG1 while the N45 glycan presented a negative impact (2-fold) regardless of the IgG1 glycoforms. 32222585 8 47 gly glycoforms 1414:1423 arg1 the IgG1 glycoforms the IgG1 glycoforms OGER IgG1 P01857 More specifically, the N162 glycan positively influenced the affinity (15-fold) for afucosylated IgG1 while the N45 glycan presented a negative impact (2-fold) regardless of the IgG1 glycoforms. 33168911 6 74 gly eNOS 871:874 arg1 Novel O-GlcNAcylation sites eNOS Novel O-GlcNAcylation sites PUBTATOR eNOS 24600 Novel O-GlcNAcylation sites of eNOS were predicted by HPLC-MS and MS/MS Ion and determined by immunoblotting. 33536395 0 41 gly glycosylation 104:116 arg1 luminal endoplasmic reticulum proteins luminal endoplasmic reticulum proteins Fterm proteins The fission yeast gmn2+ gene encodes an ERD1 homologue of Saccharomyces cerevisiae required for protein glycosylation and retention of luminal endoplasmic reticulum proteins. 33906120 10 73 gly glycosylation 1696:1708 arg2 glycosylation site mutations glycosylation site mutations site PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 33906120 10 22 gly glycosylation 1777:1789 arg2 the glycosylation modification and glycosylation sites RLK sites OGER RLK P42681 sites PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 33906120 10 22 gly glycosylation 1777:1789 arg1 LRR receptor-like serine/threonine-protein kinase kinase sites Fterm kinase sites PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 33906120 10 22 gly glycosylation 1777:1789 arg1 Beta-D-glucan exohydrolase exohydrolase sites Fterm exohydrolase sites PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 33914740 10 11 gly non-glycosylated 2112:2127 arg1 nBer e 1 nBer e 1 PUBTATOR nBer e 1 74915 Our findings suggest that the O-linked glycosylation by P. pastoris has a small but measurable effect on the in vitro antigenicity of the rBer e 1 compared to its non-glycosylated counterpart, nBer e 1, and thus may influence its applications in diagnostics and immunotherapy. 31981375 2 43 gly glycoprotein 349:360 arg1 Cynomorium songaricum Rupr glycoprotein Cynomorium songaricum Rupr glycoprotein Fterm glycoprotein In this study, single-factor experiments and response surface methodology were used to optimize the extraction of Cynomorium songaricum Rupr glycoprotein (CSG). 32518939 3 18 gly O-glycosylation 638:652 arg2 one, two, three or four potential O-glycosylation consensus sites one, two, three or four potential O-glycosylation consensus sites sites We searched in KEGG GENES database to list mouse and human proteins with an EGF-LD sequence including one, two, three or four potential O-glycosylation consensus sites. 33997889 2 46 part_of containing 442:451 arg1 engineered carrier proteins AND conserved amino acid sequences engineered carrier proteins conserved amino acid sequences Fterm Site proteins sequences Key to bioconjugation are a group of enzymes known as oligosaccharyltransferases (OTases) that transfer polysaccharides to engineered carrier proteins containing conserved amino acid sequences known as sequons. 33667901 1 38 gly attachment 289:298 arg1 serine AND β-D-N-acetylglucosamine serine and threonine residues β-D-N-acetylglucosamine serine and threonine residues Protein O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation), an essential post-translational as well as cotranslational modification, is the attachment of β-D-N-acetylglucosamine to serine and threonine residues of nucleocytoplasmic proteins. 33667901 2 15 gly profile 412:418 arg1 certain proteins proteins profile Fterm proteins An aberrant O-GlcNAc profile on certain proteins has been implicated in metabolic diseases such as diabetes and cancer. 34646892 14 18 gly c-Myc 1993:1997 arg1 Increased O-GlcNAcylation c-Myc Increased O-GlcNAcylation PUBTATOR c-Myc 4609 Increased O-GlcNAcylation of c-Myc with 30 mM glucose stabilized the c-Myc proteins. 33327053 5 26 part_of fibroin 781:787 arg1 a composite fibroin a composite Fterm Site fibroin composite In our study, we fabricated a self-healing and injectable hydrogel with a composite of chitosan, silk fibroin, and PRP (CBPGCTS-SF@PRP) for promoting diabetic wound healing. 33327053 5 49 part_of PRP 794:796 arg1 a composite PRP a composite PUBTATOR Site PRP P04156 composite In our study, we fabricated a self-healing and injectable hydrogel with a composite of chitosan, silk fibroin, and PRP (CBPGCTS-SF@PRP) for promoting diabetic wound healing. 34455356 7 26 gly nonglycosylated 1372:1386 arg1 the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A OGER NS1 Q9Y6Y0 Compared to the parental rDTMUV, the single mutants impaired viral multiplication in vitro, while the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A showed a 5-fold to 178-fold decrease in viral titers and smaller plaque sizes. 35091346 0 8 gly proteins 113:120 arg1 O-GlcNAcylation profiling proteins O-GlcNAcylation profiling Fterm proteins A chemical method for genome- and proteome-wide enrichment and O-GlcNAcylation profiling of chromatin-associated proteins. 31985438 9 60 gly glycosylated 1356:1367 arg1 the O-linked glycosylated serine sites the O-linked glycosylated serine sites serine sites Thus the proposed predictor can help in predicting the O-linked glycosylated serine sites in an efficient and accurate way. 33608772 5 12 gly glycoproteins 639:651 arg1 Serum glycoproteins Serum glycoproteins Fterm glycoproteins Serum glycoproteins of CRC patients and healthy controls were enriched by wheat germ agglutinin, a glycan binding protein specifically binds to terminal GlcNAc and sialic acid. 33924774 9 36 gly N-glycosylation 1195:1209 arg1 ANTXR1 ANTXR1 PUBTATOR ANTXR1 84168 Overall, our results identified N-glycosylation in ANTXR1 as a necessary post-translational modification for establishing stable interactions with SVV. 33914740 9 59 gly glycosylated 1766:1777 arg1 glycosylated rBer e 1 glycosylated rBer e 1 PUBTATOR rBer e 1 74915 Binding of glycosylated rBer e 1 to MR alone was found to not induce the production of IL-10 that modulates bmDC to polarise Th2 cell response by suppressing IL-12 production and DC maturation. 33546740 3 66 gly contained 505:513 arg1 THP AND arabinose THP arabinose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND mannose THP mannose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND glucuronic acid THP glucuronic acid PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND glucose THP glucose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND galacturonic acid THP galacturonic acid PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND galactose THP galactose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 31860302 4 51 gly glycoproteins 497:509 arg1 low-abundance plasma glycoproteins low-abundance plasma glycoproteins Fterm glycoproteins The absence of suitable analytical methods for in-depth and large-scale analyses of low-abundance plasma glycoproteins makes it challenging to investigate the role of glycosylation. 32046000 8 76 gly glycopeptide 1276:1287 arg2 glycopeptide glycopeptide glycopeptide Additionally, the BclA3 conjugated peptide and glycopeptide were used as antigens in an ELISA assay with serum raised against formalin-killed spores. 35091346 1 31 gly found 219:223 arg2 their cofactors AND O-Linked β-N-acetylglucosamine their cofactors O-Linked β-N-acetylglucosamine Fterm cofactors O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg2 many chromatin-associated proteins AND O-Linked β-N-acetylglucosamine many chromatin-associated proteins O-Linked β-N-acetylglucosamine Fterm proteins O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg2 their cofactors AND O-GlcNAc their cofactors O-GlcNAc Fterm cofactors O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg2 many chromatin-associated proteins AND O-GlcNAc many chromatin-associated proteins O-GlcNAc Fterm proteins O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg1 transcription factors AND O-Linked β-N-acetylglucosamine transcription factors O-Linked β-N-acetylglucosamine Fterm factors O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg1 transcription factors AND O-GlcNAc transcription factors O-GlcNAc Fterm factors O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg1 CAPs AND O-Linked β-N-acetylglucosamine CAPs O-Linked β-N-acetylglucosamine OGER CAPs Q13938 O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg1 CAPs AND O-GlcNAc CAPs O-GlcNAc OGER CAPs Q13938 O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 32510947 3 57 gly glycosite 859:867 arg2 the glycosite the glycosite glycosite Herein, we leverage CAD-induced oxocarbenium ion generation to trigger ultraviolet photodissociation (UVPD), an alternate high-energy deposition method that offers extensive fragmentation of peptides while leaving the glycosite intact. 33914740 8 49 gly present 1418:1424 arg1 rBer e 1 AND Glycans rBer e 1 Glycans PUBTATOR rBer e 1 74915 Glycans present on rBer e 1 were shown to increase the efficiency of the protein recognition and internalization by murine bone marrow-derived dendritic cells (bmDC) via C-type lectin receptors, particularly the mannose receptor (MR), compared to the non-glycosylated nBer e 1 and SFA8, a weak allergenic 2S albumin protein from sunflower seed. 33914740 8 70 gly non-glycosylated 1661:1676 arg1 the non-glycosylated nBer e 1 and SFA8 the non-glycosylated nBer e 1 and SFA8 PUBTATOR nBer e 1 74915 Glycans present on rBer e 1 were shown to increase the efficiency of the protein recognition and internalization by murine bone marrow-derived dendritic cells (bmDC) via C-type lectin receptors, particularly the mannose receptor (MR), compared to the non-glycosylated nBer e 1 and SFA8, a weak allergenic 2S albumin protein from sunflower seed. 33914740 8 70 gly non-glycosylated 1661:1676 arg1 a weak allergenic 2S albumin protein a weak allergenic 2S albumin protein OGER 2S albumin protein P07724 Glycans present on rBer e 1 were shown to increase the efficiency of the protein recognition and internalization by murine bone marrow-derived dendritic cells (bmDC) via C-type lectin receptors, particularly the mannose receptor (MR), compared to the non-glycosylated nBer e 1 and SFA8, a weak allergenic 2S albumin protein from sunflower seed. 33317052 0 26 gly Decorin 34:40 arg1 Glycosaminoglycan Modification Decorin Glycosaminoglycan Modification PUBTATOR Decorin 13179 Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly. 33317052 0 39 gly Modification 18:29 arg1 Decorin AND Glycosaminoglycan Modification Decorin Glycosaminoglycan Modification PUBTATOR Decorin 13179 Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly. 34435655 6 17 gly p38 MAPK 1208:1215 arg1 global O‑GlcNAcylation p38  global O‑GlcNAcylation p38  OGA inhibition increased global O‑GlcNAcylation and the phosphorylation of p38 MAPK, ERK1/2 and CaMKII, whereas OGT blockade had the opposite effects. 34435655 6 54 gly ERK1/2 1218:1223 arg1 global O‑GlcNAcylation ERK1/2 global O‑GlcNAcylation PUBTATOR ERK1/2 50689 OGA inhibition increased global O‑GlcNAcylation and the phosphorylation of p38 MAPK, ERK1/2 and CaMKII, whereas OGT blockade had the opposite effects. 32499112 7 54 gly O-glycosylation 1152:1166 arg2 22 more possible Ser/Thr O-glycosylation sites 22 more possible Ser/Thr O-glycosylation sites sites In this study, we not only validate the previously described three-serine residues (208, 238 and 400) as Yin-Yang sites but also predicted 22 more possible Ser/Thr O-glycosylation sites. 34544801 4 9 gly glycosylation 658:670 arg1 the total circulating IgG the total circulating IgG Cterm IgG It specifically included adaptive immune receptor repertoire sequencing to quantify and characterize N-linked glycosylation sites in the circulating BCR repertoire, proteomics to examine glycosylation patterns of the total circulating IgG, and an exploration of human-derived recombinant autoantibodies, which were studied with mass spectrometry and Ag binding assays to respectively confirm occupation of glycosylation sites and determine whether they alter binding. 34544801 4 34 gly glycosylation 581:593 arg2 N-linked glycosylation sites N-linked glycosylation sites sites It specifically included adaptive immune receptor repertoire sequencing to quantify and characterize N-linked glycosylation sites in the circulating BCR repertoire, proteomics to examine glycosylation patterns of the total circulating IgG, and an exploration of human-derived recombinant autoantibodies, which were studied with mass spectrometry and Ag binding assays to respectively confirm occupation of glycosylation sites and determine whether they alter binding. 34544801 4 60 gly glycosylation 877:889 arg2 glycosylation sites glycosylation sites sites It specifically included adaptive immune receptor repertoire sequencing to quantify and characterize N-linked glycosylation sites in the circulating BCR repertoire, proteomics to examine glycosylation patterns of the total circulating IgG, and an exploration of human-derived recombinant autoantibodies, which were studied with mass spectrometry and Ag binding assays to respectively confirm occupation of glycosylation sites and determine whether they alter binding. 34455356 2 31 part_of N-linked 360:367 arg1 three potential predicted N-linked glycosylation sites N three potential predicted N-linked glycosylation sites Cterm Site N sites DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and 207. 34455356 2 0 part_of contains 325:332 arg1 NS1 AND three potential predicted N-linked glycosylation sites NS1 three potential predicted N-linked glycosylation sites OGER Site NS1 Q9Y6Y0 sites DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and 207. 34455356 2 0 part_of contains 325:332 arg1 DTMUV nonstructural protein 1 AND three potential predicted N-linked glycosylation sites DTMUV nonstructural protein 1 three potential predicted N-linked glycosylation sites Fterm Site protein 1 sites DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and 207. 34559939 2 45 gly glycoprotein 383:394 arg1 glycoprotein composition glycoprotein composition Fterm glycoprotein Exosomes have characteristic protein and lipid composition; however, the results concerning glycoprotein composition and glycosylation are scarce. 33451024 4 3 gly N-glycosylation 711:725 arg2 conserved N-glycosylation sites conserved N-glycosylation sites sites Results from NetNGlyc program analyzed the H5 hemagglutinin sequences of isolates during 2004-2020, revealing that seven and two conserved N-glycosylation sites were detected in HA1 and HA2 domain, respectively. 33451024 4 33 gly seven 687:691 arg1 conserved N-glycosylation sites conserved N-glycosylation sites sites Results from NetNGlyc program analyzed the H5 hemagglutinin sequences of isolates during 2004-2020, revealing that seven and two conserved N-glycosylation sites were detected in HA1 and HA2 domain, respectively. 32188979 2 46 gly glycosylation 272:284 arg1 Hyl Hyl OGER Hyl P42679 Among these PTMs, glycosylation of hydroxylysine (Hyl) is the most complicated. 33361116 0 33 gly Afucosylated 0:11 arg1 Afucosylated IgG Afucosylated IgG Cterm IgG Afucosylated IgG characterizes enveloped viral responses and correlates with COVID-19 severity. 34056367 5 64 gly glycoproteins 854:866 arg1 membrane glycoproteins membrane glycoproteins Fterm glycoproteins We are specifically interested in the properties of membrane glycoproteins, which are key components in a number of different disease states. 33073165 6 10 part_of α3β1 1174:1177 arg1 the α3β1 integrin metal ion-dependent adhesion site α3β1 the α3β1 integrin metal ion-dependent adhesion site Cterm Site α3β1 site Molecular modeling of integrin binding indicated that Hyl glycosylation directly impacted the association between the α3β1 integrin metal ion-dependent adhesion site (MIDAS) and the receptor binding site within type IV collagen. 33073165 6 61 part_of integrin 1179:1186 arg1 the α3β1 integrin metal ion-dependent adhesion site integrin the α3β1 integrin metal ion-dependent adhesion site Fterm Site integrin site Molecular modeling of integrin binding indicated that Hyl glycosylation directly impacted the association between the α3β1 integrin metal ion-dependent adhesion site (MIDAS) and the receptor binding site within type IV collagen. 34489534 3 16 part_of channels 522:529 arg1 the 'cap' region Piezo1 channels the 'cap' region PUBTATOR Site Piezo1 channels 9780 region Here we show that N-linked glycosylation of two highly conserved asparagine residues in the 'cap' region of mechanosensitive Piezo1 channels are necessary for the mature protein to reach the plasma membrane. 35058964 1 11 gly glycoprotein 189:200 arg1 the small integrin-binding ligand N-linked glycoprotein (SIBLING) family the small integrin-binding ligand N-linked glycoprotein (SIBLING) family Fterm glycoprotein Background: SPP1, secreted phosphoprotein 1, is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. 34023382 1 14 part_of proteins 297:304 arg1 asparagine residues proteins asparagine residues Fterm AminoAcid proteins asparagine residues Oligosaccharyltransferase (OST) catalyzes the central step in N-linked protein glycosylation, the transfer of a preassembled oligosaccharide from its lipid carrier onto asparagine residues of secretory proteins. 32958677 7 48 gly attached 1310:1317 arg1 misfolded AAT AND N-glycans misfolded AAT N-glycans PUBTATOR AAT 5265 Also, N-glycans attached to misfolded AAT are not required for accelerated degradation mediated by the unconventional system, further demonstrating its catalysis-independent nature. 32730198 11 5 part_of SURF-ANA1T 1588:1597 arg1 genomic sequences SURF-ANA1T genomic sequences Cterm Site SURF-ANA1T sequences The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genomic sequences of strain SURF-ANA1T are KU359248 and GCF 009768935.1, respectively. 33272761 8 13 gly fucosylated 1012:1022 arg1 patatin patatin PUBTATOR patatin 102577633 Our findings suggest that patatin, a fucosylated glycoprotein, could potentially be used as a naturalactiveconstituent with anti-obesity effects. 33272761 8 13 gly fucosylated 1012:1022 arg1 a fucosylated glycoprotein a fucosylated glycoprotein Fterm glycoprotein Our findings suggest that patatin, a fucosylated glycoprotein, could potentially be used as a naturalactiveconstituent with anti-obesity effects. 33272761 8 15 gly glycoprotein 1024:1035 arg1 patatin patatin PUBTATOR patatin 102577633 Our findings suggest that patatin, a fucosylated glycoprotein, could potentially be used as a naturalactiveconstituent with anti-obesity effects. 33272761 8 15 gly glycoprotein 1024:1035 arg1 a fucosylated glycoprotein a fucosylated glycoprotein Fterm glycoprotein Our findings suggest that patatin, a fucosylated glycoprotein, could potentially be used as a naturalactiveconstituent with anti-obesity effects. 33024038 11 98 gly O-glycosylation 2120:2134 arg2 O-glycosylation sites O-glycosylation sites sites Here, we show that CpaA cleaves multiple targets precisely at O-glycosylation sites preceded by a Pro residue. 32709339 13 3 gly fragment 1848:1855 arg1 sugar fragment ions sugar fragment ions Additionally, another logistic regression model completely differentiated between parent and sugar fragment ions. 32910282 9 3 gly β2 1790:1791 arg1 O-GlcNAcylated 2 subunits O-GlcNAcylated PUBTATOR 2 subunits 20454 Additionally, we show that the Cav α1 and β2 subunits are O-GlcNAcylated while α2δ1 is not. 34342946 1 15 gly glycoprotein 245:256 arg1 an abundant type I transmembrane glycoprotein an abundant type I transmembrane glycoprotein Fterm glycoprotein Bovine butyrophilin (BTN1A1) is an abundant type I transmembrane glycoprotein exposed on the surface of milk fat globules. 34342946 1 15 gly glycoprotein 245:256 arg1 Bovine butyrophilin Bovine butyrophilin Fterm butyrophilin Bovine butyrophilin (BTN1A1) is an abundant type I transmembrane glycoprotein exposed on the surface of milk fat globules. 35004852 7 19 gly glycoprotein 1562:1573 arg1 decentralized glycoprotein production decentralized glycoprotein production Fterm glycoprotein Overall, this work represents a first-in-kind glycosylation-on-a-chip prototype that could find use as a laboratory tool for mechanistic dissection of the protein glycosylation process as well as a biomanufacturing platform for small batch, decentralized glycoprotein production. 34227365 8 55 gly N-glycoprotein/N-glycopeptide 1819:1847 arg2 N-glycoprotein/N-glycopeptide enrichment N-glycoprotein/N-glycopeptide enrichment N-glycoprotein/N-glycopeptide Liquid chromatography-mass spectrometry (LC-MS) is an analytical technique widely used for the large-scale profiling of proteomes in biological systems, and the enrichment of N-glycopeptides is a prerequisite for their detection by MS.In this study, we established an approach based on hydrophilic interaction chromatography (HILIC) by optimizing the activation, cleaning, and elution processes of the enrichment method, for instance through the optimization of particle size and solvent composition, and investigated the identification number, selectivity, and stability of N-glycoprotein/N-glycopeptide enrichment under different experimental conditions. 34227365 8 86 gly N-glycopeptides 1419:1433 arg2 N-glycopeptides N-glycopeptides N-glycopeptides Liquid chromatography-mass spectrometry (LC-MS) is an analytical technique widely used for the large-scale profiling of proteomes in biological systems, and the enrichment of N-glycopeptides is a prerequisite for their detection by MS.In this study, we established an approach based on hydrophilic interaction chromatography (HILIC) by optimizing the activation, cleaning, and elution processes of the enrichment method, for instance through the optimization of particle size and solvent composition, and investigated the identification number, selectivity, and stability of N-glycoprotein/N-glycopeptide enrichment under different experimental conditions. 31969392 3 40 gly fucosylated 508:518 arg1 their highly modified glycosaminoglycans their highly modified glycosaminoglycans Other than their highly modified glycosaminoglycans (e.g. fucosylated chondroitin sulfate and fucoidan), nothing is known about their protein-linked glycosylation. 31919391 4 44 gly glycoproteins 1072:1084 arg1 195 different glycoproteins 195 different glycoproteins Fterm glycoproteins Moreover, Fe3O4-GO@PDA-Chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. 31919391 4 46 gly utilized 905:912 arg2 Fe3O4-GO@PDA-Chitosan nanocomposites Fe3O4-GO@PDA-Chitosan nanocomposites nanocomposites Moreover, Fe3O4-GO@PDA-Chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. 31919391 4 50 gly glycopeptides 936:948 arg2 glycopeptides glycopeptides glycopeptides Moreover, Fe3O4-GO@PDA-Chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. 31919391 4 54 gly glycosylation 1094:1106 arg2 458 glycosylation sites 458 glycosylation sites sites Moreover, Fe3O4-GO@PDA-Chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. 31919391 4 64 gly glycopeptides 1030:1042 arg2 393 N-linked glycopeptides 393 N-linked glycopeptides glycopeptides Moreover, Fe3O4-GO@PDA-Chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. 32518069 5 9 gly glycoproteins 881:893 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins We characterized site-specific alteration in sialylation on N-linked glycoproteins in isolated rat nerve terminals after brief depolarization using quantitative sialiomics. 32522876 10 72 gly glycosylation 1697:1709 arg1 chromatin factors chromatin factors Fterm factors These data revealed that O-linked glycosylation of chromatin factors is essential for the transcriptional repression of methylated retrotransposons. 34202747 9 22 part_of protein 1396:1402 arg1 the active site protein the active site Fterm Site protein site Molecular docking was further performed to study the combination mode of the GMABX-PA into the active site of two lung cancer proteins (5XNV, 2EB2) and a blood cancer protein (2M6N). 34202747 9 71 part_of proteins 1355:1362 arg1 the active site proteins the active site Fterm Site proteins site Molecular docking was further performed to study the combination mode of the GMABX-PA into the active site of two lung cancer proteins (5XNV, 2EB2) and a blood cancer protein (2M6N). 34622651 10 94 gly glycopeptides 1719:1731 arg2 intact glycopeptides intact glycopeptides glycopeptides In summary, our integrated platform can serve as a reliable quantitative tool for characterizing intact glycopeptides isolated from complex biological samples to assist our understanding of biological functions of N-linked glycosylation. 32252658 8 24 gly glycosylation 1143:1155 arg2 a new N-linked glycosylation site a new N-linked glycosylation site site In mutated SERPINC1 protein a new N-linked glycosylation site is formed, however, it is unclear if the glycosylation at 219-221 site is possible. 32252658 8 46 gly glycosylation 1203:1215 arg1 the glycosylation at 219-221 site site site In mutated SERPINC1 protein a new N-linked glycosylation site is formed, however, it is unclear if the glycosylation at 219-221 site is possible. 34711323 1 47 gly O-glycosylation 178:192 arg2 one O-glycosylation sites one O-glycosylation sites sites Recombinant human erythropoietin (EPO) is a complex therapeutic glycoprotein with three N- and one O-glycosylation sites. 34711323 1 62 gly glycoprotein 143:154 arg1 a complex therapeutic glycoprotein a complex therapeutic glycoprotein Fterm glycoprotein Recombinant human erythropoietin (EPO) is a complex therapeutic glycoprotein with three N- and one O-glycosylation sites. 34711323 1 62 gly glycoprotein 143:154 arg1 Recombinant human erythropoietin Recombinant human erythropoietin PUBTATOR erythropoietin 2056 Recombinant human erythropoietin (EPO) is a complex therapeutic glycoprotein with three N- and one O-glycosylation sites. 33454535 4 24 gly glycosylation 582:594 arg2 N-linked glycosylation sites N-linked glycosylation sites sites From segments of ion channel proteins centered around N-linked glycosylation sites, the amino acid embedding vectors of each residue were concatenated to create features for prediction. 32157140 5 37 gly N-glycosylation 679:693 arg1 endogenous Gpr176 endogenous Gpr176 PUBTATOR Gpr176 Q14439 Peptide-N-glycosidase treatment of mouse hypothalamus extracts revealed that endogenous Gpr176 undergoes N-glycosylation. 32393178 6 74 part_of HA 1249:1250 arg1 the HA receptor binding site HA the HA receptor binding site Cterm Site HA None site Furthermore, the implicated mutations are predominantly located at five reported antibody-binding sites, and within or close to the HA receptor binding site. 34363524 9 24 part_of containing 1266:1275 arg1 RAB6-interacting golgin AND zinc finger (Znf) domain RAB6-interacting golgin zinc finger (Znf) domain OGER Site RAB6-interacting golgin Q5T7V8 domain Interestingly, protein-protein interaction analysis demonstrated some GLPs are associated with RAB6-interacting golgin, oligopeptide transmembrane transporter activity, beta-glucuronidase activity, protein N-linked glycosylation, multi-pass membrane protein, and proteins containing zinc finger (Znf) domain and RING (really interesting new gene)-type zinc finger domains. 33182133 10 37 gly polysaccharides 1492:1506 arg1 proteins proteins polysaccharides Fterm proteins Our results suggested that higher polysaccharides, a higher β-sheets band in proteins, and lower mineral surface-adhesive and viscoelastic properties benefited the aerobic sludge granulation process and the granule maintenance. 34455356 0 45 part_of N-linked 24:31 arg1 N-linked glycosylation sites N N-linked glycosylation sites Cterm Site N sites N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N-linked glycosylation sites NS1 N-linked glycosylation sites OGER Site NS1 Q9Y6Y0 sites N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 45 part_of N-linked 24:31 arg1 N130 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 45 part_of N-linked 24:31 arg1 N207 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 45 part_of N-linked 24:31 arg1 N207 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N130 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N207 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N207 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 33997889 1 5 gly attached 157:164 arg2 carrier proteins AND polysaccharides carrier proteins polysaccharides Fterm proteins Bioconjugate vaccines, consisting of polysaccharides attached to carrier proteins, are enzymatically generated using prokaryotic glycosylation systems in a process termed bioconjugation. 33682335 9 47 gly glycoprotein 1228:1239 arg1 the glycoprotein glycoprotein The more globular-shaped glycan Fterm glycoprotein The more globular-shaped glycan of the glycoprotein suggests that a thicker bonded phase might improve selectivity. 34284575 8 74 gly O-glycosylation 1039:1053 arg1 recombinant SARS-CoV-2 recombinant SARS-CoV-2 OGER SARS P49591 We utilized WR-SiO2 to comprehensively analyze the O-glycosylation profile of recombinant SARS-CoV-2 S. Twenty-seven O-glycosylation sites including 18 unambiguous sites are identified on SARS-CoV-2 S. 34284575 8 34 gly O-glycosylation 1105:1119 arg2 S. Twenty-seven O-glycosylation sites sites sites We utilized WR-SiO2 to comprehensively analyze the O-glycosylation profile of recombinant SARS-CoV-2 S. Twenty-seven O-glycosylation sites including 18 unambiguous sites are identified on SARS-CoV-2 S. 33552834 6 59 gly glycosylation 1294:1306 arg2 N-linked glycosylation sites N-linked glycosylation sites sites Based on the loss of crucial interactions and the absence of N-linked glycosylation sites we categorized Felis catus, Equus caballus, Panthera tigris altaica, as highly susceptible while Oryctolagus cuniculus, Bos Tauras, Ovis aries and Capra hircus as moderately susceptible species for infection. 33886622 9 25 part_of PmCOX 1475:1479 arg1 Three potential glycosylation sites COX Three potential glycosylation sites OGER Site COX sites Three potential glycosylation sites of PmCOX were identified at N79, N170 and N424. 33547363 8 20 gly glycosylation 1354:1366 arg2 N-linked glycosylation sites N-linked glycosylation sites sites The presence of N-linked glycosylation sites in the iron starvation-induced protein 1a (ISIP1a) confirmed its predicted topology, contributing to the biochemical characterization of ISIP1 proteins. 34544801 5 13 part_of IgG-VN-Glyc 970:980 arg1 IgG-VN-Glyc motifs IgG IgG-VN-Glyc motifs Cterm Site IgG motifs We found that the frequency of IgG-VN-Glyc motifs was increased in the total BCR repertoire of patients with MG when compared with healthy donors. 32776095 5 22 gly sialylated 851:860 arg1 sialylated core 1 glycans sialylated core 1 glycans MMP10, the principal gene upregulated when cells carrying sialylated core 1 glycans were stimulated with EGF, is also upregulated in ER-positive breast carcinoma reported to express high levels of ST3Gal1 and hence mainly core 1 sialylated O-glycans. 32776095 5 30 gly sialylated 1022:1031 arg1 core 1 sialylated O-glycans core 1 sialylated O-glycans MMP10, the principal gene upregulated when cells carrying sialylated core 1 glycans were stimulated with EGF, is also upregulated in ER-positive breast carcinoma reported to express high levels of ST3Gal1 and hence mainly core 1 sialylated O-glycans. 34030043 3 32 part_of has 469:471 arg1 TIGIT AND N32 TIGIT residues, N32 and N101 PUBTATOR SpecificSite TIGIT 201633 residues, N32 and N101 TIGIT has two N-glycosylation residues, N32 and N101. 34030043 3 32 part_of has 469:471 arg1 TIGIT AND two N-glycosylation residues TIGIT residues, N32 and N101 PUBTATOR SpecificSite TIGIT 201633 residues, N32 and N101 TIGIT has two N-glycosylation residues, N32 and N101. 34030043 3 32 part_of has 469:471 arg1 TIGIT AND two N-glycosylation residues TIGIT residues, N32 and N101 PUBTATOR SpecificSite TIGIT 201633 residues, N32 and N101 TIGIT has two N-glycosylation residues, N32 and N101. 32892942 6 76 gly N-glycopeptides 811:825 arg2 N-glycopeptides identification N-glycopeptides identification N-glycopeptides The detection sensitivity for N-glycopeptides identification by MALDI-TOF MS was as low as 10 fmol for tryptic digest of standard human IgG. 33242079 2 38 gly sialylated 377:386 arg1 Alpha 2,6 sialylated N-glycans Alpha 2,6 sialylated N-glycans Alpha 2,6 sialylated N-glycans, one of representative sialylation forms, are synthesized by St6gal1 or St6gal2 gene products in humans and mice. 33242079 2 38 gly sialylated 377:386 arg1 representative sialylation forms representative sialylation forms Alpha 2,6 sialylated N-glycans, one of representative sialylation forms, are synthesized by St6gal1 or St6gal2 gene products in humans and mice. 33914740 6 3 gly glycosylation 1081:1093 arg1 rBer e 1 rBer e 1 PUBTATOR rBer e 1 74915 The glycosylation on rBer e 1 was shown to elicit varying degree of antigenicity by binding to different combination of human leukocyte antigens (HLA) at different frequencies compared to nBer e 1 when tested using human DC-T cell assay. 33647064 9 18 gly glycosylation 1192:1204 arg2 these glycosylation sites these glycosylation sites sites On the other hand, disruption of these glycosylation sites on wild-type MACV GPC rendered the virus substantially attenuated in vivo and also more susceptible to antibody neutralization, while loss of these glycans did not affect virus growth in cultured cells. 34895221 6 7 part_of VIH-kinase 1190:1199 arg1 the functional VIH-kinase domains VIH the functional VIH-kinase domains Cterm Site VIH domains We demonstrate that wheat VIH proteins can utilize InsP7 as the substrate to produce InsP8, a process that requires the functional VIH-kinase domains. 34559939 2 5 part_of glycoprotein 383:394 arg1 glycoprotein composition glycoprotein glycoprotein composition Fterm Site glycoprotein position Exosomes have characteristic protein and lipid composition; however, the results concerning glycoprotein composition and glycosylation are scarce. 32241176 9 86 part_of GBP 1608:1610 arg1 the molecular composition GBP the molecular composition PUBTATOR Site GBP 54972 position The cytotoxicity of doxorubicin positively correlated with the molecular composition of GBP. 31936366 6 47 gly c-Myc 1326:1330 arg1 O-GlcNAcylation-dependent expression Myc O-GlcNAcylation-dependent expression OGER Myc P01108 These results strongly suggest that O-GlcNAcylation-dependent expression of c-Myc represents a new regulatory component of pre-B cell proliferation, as well as a potential therapeutic target for the treatment of pre-B cell-derived leukemia. 34418796 2 33 gly glycosylated 201:212 arg1 IBAV NS3 protein IBAV NS3 protein Fterm protein We have previously shown that IBAV NS3 protein is highly glycosylated and that tunicamycin, an inhibitor of N-linked glycosylation, suppressed NS3 glycosylation and viral propagation. 34030043 4 5 part_of TIGIT 544:548 arg1 N101 TIGIT N101 PUBTATOR SpecificSite TIGIT 201633 N101 N-glycosylation on N101 of TIGIT and, to less extent, on N32, play potent roles in PVR binding. 32439055 4 3 gly 68 N-glycosylation 731:748 arg2 68 N-glycosylation sites 68 N-glycosylation sites sites As to human serum samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites were finally detected, which showed the good ability of the nanosheets material to enrich N-linked glycopeptides from complex human serum samples. 32439055 4 6 gly glycoproteins 679:691 arg1 41 glycoproteins 41 glycoproteins Fterm glycoproteins As to human serum samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites were finally detected, which showed the good ability of the nanosheets material to enrich N-linked glycopeptides from complex human serum samples. 32439055 4 22 gly glycopeptides 855:867 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides As to human serum samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites were finally detected, which showed the good ability of the nanosheets material to enrich N-linked glycopeptides from complex human serum samples. 32439055 4 47 gly glycopeptides 700:712 arg2 66 glycopeptides 66 glycopeptides glycopeptides As to human serum samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites were finally detected, which showed the good ability of the nanosheets material to enrich N-linked glycopeptides from complex human serum samples. 33559614 9 57 part_of domain 1516:1521 arg1 residues domain residues residues The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 33559614 9 57 part_of domain 1516:1521 arg1 His242 Gln238, Gln239, His242 and Ser441 Gln238, Gln239, His242 and Ser441 The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 33559614 9 57 part_of domain 1516:1521 arg1 Ser441 Gln238, Gln239, His242 and Ser441 Gln238, Gln239, His242 and Ser441 The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 33559614 9 57 part_of domain 1516:1521 arg1 Gln238 Gln238, Gln239, His242 and Ser441 Gln238, Gln239, His242 and Ser441 The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 33559614 9 57 part_of domain 1516:1521 arg1 Ser441 Gln238, Gln239, His242 and Ser441 Gln238, Gln239, His242 and Ser441 The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 33559614 9 57 part_of domain 1516:1521 arg1 Gln238 Gln238, Gln239, His242 and Ser441 Gln238, Gln239, His242 and Ser441 The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 33559614 9 57 part_of domain 1516:1521 arg1 Gln238 Gln238, Gln239, His242 and Ser441 Gln238, Gln239, His242 and Ser441 The other is formed around the three hydroxyl groups of the compound, with the involvement of residues mainly from the cap domain, including Gln238, Gln239, His242 and Ser441. 34734627 7 9 gly glycosylation 1192:1204 arg2 glycosylation site occupancy glycosylation site occupancy site Our results provide further insight into the important role of the endoplasmic reticulum redox conditions in glycosylation site occupancy and demonstrate a link between redox conditions in the cytosol and glycosylation efficiency. 32441096 5 51 gly core-fucosylated 960:975 arg1 core-fucosylated N-glycans core-fucosylated N-glycans On tissues, this results in a mass shift of 349.137 a.m.u. for core-fucosylated N-glycans when compared to N-glycans released with standard PNGaseF. 34227365 22 11 gly N-glycopeptides 3641:3655 arg2 N-glycopeptides N-glycopeptides N-glycopeptides The expression levels of N-glycopeptides between the two groups suggested a clear gender difference. 34644163 5 5 gly glycosylated 766:777 arg1 The GFP-CTDCHU_2708 fusion protein The GFP-CTDCHU_2708 fusion protein Fterm protein The GFP-CTDCHU_2708 fusion protein was found to be glycosylated in the periplasm, with a molecular mass about 5 kDa higher than that predicted from its sequence. 33559614 8 25 part_of domain 1298:1303 arg1 residues domain residues residues One is formed around the carboxyl group of the gallic acid and involves residues from the hydrolase-fold domain, including those from the catalytic triad, which consists of Ser206, His485 and Asp439. 34304571 0 36 part_of EWS 62:64 arg1 the RNA-Binding Protein EWS N-Terminal Low Complexity Region EWS the RNA-Binding Protein EWS N-Terminal Low Complexity Region PUBTATOR EWS 2130 O-Linked-N-Acetylglucosaminylation of the RNA-Binding Protein EWS N-Terminal Low Complexity Region Reduces Phase Separation and Enhances Condensate Dynamics. 31869211 1 11 gly N-glycopeptide 271:284 arg2 efficient N-glycopeptide enrichment efficient N-glycopeptide enrichment N-glycopeptide Leaf-like hollow cobalt sulfides with a sulfur-gold-cysteine (S-Au-Cys) structure on the surface have been synthesized for efficient N-glycopeptide enrichment. 34725712 3 58 gly glycosite 514:522 arg2 glycosite glycosite glycosite Here, we report the effects of iterative truncations to the TPR domain of OGT on substrate and glycosite selection with the model protein GFP-JunB and the surrounding O-GlcNAc proteome in U2OS cells. 32185163 7 2 gly fucosylated 1038:1048 arg1 the remaining antennary fucosylated glycans the remaining antennary fucosylated glycans The core fucosylated glycans are thus depleted while the remaining antennary fucosylated glycans are quantitated. 32185163 7 18 gly fucosylated 970:980 arg1 The core fucosylated glycans The core fucosylated glycans The core fucosylated glycans are thus depleted while the remaining antennary fucosylated glycans are quantitated. 32117864 11 0 gly glycosites 2012:2021 arg2 all glycosites all glycosites glycosites Sites of decreased sialylation were minor in the MCF10A (<25% of all glycosites) and T-47D (<15%) cells but dominated in the MDA-MB-231 line (~60%) suggesting that excess sialic acid could be detrimental in advanced cancer and cancer cells can evolve mechanisms to guard against hypersialylation. 34681302 7 86 gly proteins 1327:1334 arg1 subcutaneous fat fat proteins PUBTATOR fat 12491 Furthermore, serum lipopolysaccharide-binding proteins (LBP), adiponectin level, and pro-inflammation gene expression in the colon and subcutaneous fat were inhibited, whereas anti-inflammation gene expression was improved, reflecting SeCMP-200 might mitigate obese-induced inflammation. 33206527 9 163 part_of CES1 1788:1791 arg1 the N-linked glycosyl group at Asn79 CES1 the N-linked glycosyl group at Asn79 PUBTATOR AminoAcid CES1 1066 Asn79 Given that the N-linked glycosyl group at Asn79 (N79 glycan) of CES1 is known to influence CES1 enzyme activity, we hypothesized that the post-translational modification of CES1 at N79 may be linked to its antiproliferative activity. 33451024 3 14 gly N-glycosylation 521:535 arg2 the optimal DC-SIGN interacting N-glycosylation sites the optimal DC-SIGN interacting N-glycosylation sites sites This study aims to identify the optimal DC-SIGN interacting N-glycosylation sites in HA proteins of H5N1-AIVs. 34215103 0 98 gly glycopeptides 101:113 arg2 endogenous glycopeptides endogenous glycopeptides glycopeptides Bowl-like mesoporous polydopamine with size exclusion for highly selective recognition of endogenous glycopeptides. 33420984 7 52 gly deglycosylated 1772:1785 arg1 deglycosylated peptides deglycosylated peptides peptides The IMAC flow-through and acidic elution are subsequently subjected to a next round of TiO2 enrichment for further separation of mono-phosphopeptides from deglycosylated peptides. 31724933 4 29 gly glycoprotein 569:580 arg1 the soybean vein necrosis virus N glycoprotein the soybean vein necrosis virus N glycoprotein Fterm glycoprotein In this study, peptides derived from the soybean vein necrosis virus N glycoprotein were utilized to identify critical regions in virus-vector interactions. 31724933 4 31 gly utilized 587:594 arg2 peptides peptides peptides In this study, peptides derived from the soybean vein necrosis virus N glycoprotein were utilized to identify critical regions in virus-vector interactions. 34887587 7 48 part_of eIF3a 1036:1040 arg1 the modification site eIF3a the modification site PUBTATOR Site eIF3a 8669 site Eliminating the modification site from eIF3a via CRISPR genome editing induces ATF4 reinitiation even under the nutrient-rich condition. 32337418 1 5 part_of G 187:187 arg1 the fragment crystallizable (Fc) domain immunoglobulin G the fragment crystallizable (Fc) domain PUBTATOR Site immunoglobulin G 16059 domain N-Linked glycosylation of the fragment crystallizable (Fc) domain of immunoglobulin G (IgG) is considered a significant modulator of antibody functions, which is known to be subclass-specific. 31860302 8 15 gly glycoproteins 1378:1390 arg1 several low-abundance plasma glycoproteins several low-abundance plasma glycoproteins Fterm glycoproteins Additionally, several low-abundance plasma glycoproteins were identified, including SVEP1 (∼0.54 ng/mL), F8 (∼0.83 ng/mL), and ADAMTS13 (∼1.2 ng/mL). 32157140 6 69 gly N-glycosylation 749:763 arg2 four conserved asparagine residues four conserved asparagine residues asparagine residues Using a heterologous expression system, we show that N-glycosylation occurs at four conserved asparagine residues in the N-terminal region of Gpr176. 33647064 6 82 gly N166 746:749 arg1 N-linked glycans N83 and N166 N-linked glycans N83 and N166 Interestingly, mutations resulting in acquisition of N-linked glycans at GPC N83 and N166 frequently occurred in late stages of the infection. 33647064 6 89 gly N83 738:740 arg1 N-linked glycans N83 and N166 N-linked glycans N83 and N166 Interestingly, mutations resulting in acquisition of N-linked glycans at GPC N83 and N166 frequently occurred in late stages of the infection. 33168911 8 26 gly eNOS 1085:1088 arg1 low glucose-associated activation eNOS low glucose-associated activation PUBTATOR eNOS 24600 In BAECs and rat thoracic aorta, low glucose-associated activation of eNOS was accompanied by elevated O-GlcNAcylation, which did not affect O-linked serine phosphorylation at 1179/1177 residues. 33232205 9 60 gly glycoprotein 1833:1844 arg1 the native S glycoprotein the native S glycoprotein PUBTATOR S glycoprotein 43740568 This study revealed that the native S glycoprotein stimulated neutralizing antibodies, while bacterially-expressed S fragments did not. 33206527 12 6 gly glycosylation 2461:2473 arg2 Asn79 Asn79 Asn79 Thus our results suggest that CES1 exerts an antiproliferative effect in liver cancer cells and that the single N-linked glycosylation at Asn79 plays a potential regulatory role. 33065162 5 1 gly activity 922:929 arg1 RBPR2 RBPR2 activity PUBTATOR RBPR2 74152 Immunoprecipitation and dual fluorescence staining were used to explore O-GlcNAc-modified RBPR2 and RBP4 binding activity on RBPR2. 33346724 0 29 gly glycoprotein 78:89 arg1 glycoprotein N-linked glycans glycoprotein N-linked glycans Chronic exposure to low doses of ionizing radiation impacts the processing of glycoprotein N-linked glycans in Medaka (Oryzias latipes). 32975831 7 13 gly glycopeptides 1028:1040 arg2 glycopeptides glycopeptides glycopeptides After purification and protein digestion, glycopeptides are analyzed by LC/ESI-MS. 34069226 2 66 part_of contains 230:237 arg1 The Fc domain AND The Fc domain The Fc domain The Fc domain an N-linked oligosaccharide domain The Fc domain of IgG antibodies contains an N-linked oligosaccharide at position 297. 34105348 1 12 gly threonine 237:245 arg1 O-GlcNAcylation threonine O-GlcNAcylation threonine O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 34105348 1 16 gly proteins 250:257 arg1 O-GlcNAcylation proteins O-GlcNAcylation Fterm proteins O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 34105348 1 51 gly O-GlcNAc 186:193 arg1 O-GlcNAcylation O-GlcNAc O-GlcNAcylation Cterm O-GlcNAc O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 34105348 1 76 gly serine 227:232 arg1 O-GlcNAcylation serine O-GlcNAcylation serine O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 32892942 8 6 part_of 172 N-glycoproteins 1227:1245 arg1 598 unique N-glycopeptides 172 N-glycoproteins 598 unique N-glycopeptides Fterm Site 172 N-glycoproteins N-glycopeptides Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 32892942 8 6 part_of 172 N-glycoproteins 1227:1245 arg1 344 unique N-glycosites 172 N-glycoproteins 344 unique N-glycosites Fterm Site 172 N-glycoproteins N-glycosites Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 32892942 8 10 part_of N-glycosites 1179:1190 arg1 172 N-glycoproteins 172 N-glycoproteins N-glycosites Fterm Site 172 N-glycoproteins N-glycosites Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 34828995 7 19 gly glutamic 778:785 arg1 Glucose glutamic acid, leucine Glucose glutamic acid, leucine Glucose, galacturonic acid, aspartate, glutamic acid, leucine, Ca, K, Al, Mg, Na and Fe are the main monomer components. 34828995 7 19 gly glutamic 778:785 arg1 galacturonic acid glutamic acid, leucine galacturonic acid glutamic acid, leucine Glucose, galacturonic acid, aspartate, glutamic acid, leucine, Ca, K, Al, Mg, Na and Fe are the main monomer components. 35004852 1 6 gly glycoproteins 216:228 arg1 glycoproteins glycoproteins Fterm glycoproteins In recent years, cell-free synthetic glycobiology technologies have emerged that enable production and remodeling of glycoproteins outside the confines of the cell. 32931036 3 60 part_of mGlu7 496:500 arg1 the extracellular domain mGlu7 the extracellular domain Cterm Site mGlu7 2917 domain However, the functional importance of the extracellular domain of mGlu7 has not yet been characterized. 31529722 12 58 part_of acyl-chain 2000:2009 arg1 the acyl-chain composition chain the acyl-chain composition OGER Site chain P01308 position CONCLUSIONS Our study demonstrates the key role of hepatic Elovl6 in the regulation of the acyl-chain composition of ceramide and that C18:0-ceramide is a potent regulator of hepatic insulin signaling linked to Pnpla3-mediated NAFLD. 34536471 6 8 gly proteins 1008:1015 arg1 polysaccharides proteins polysaccharides Fterm proteins The cyanobacterium was then grown on a larger scale (10 L) to evaluate the characteristics of the produced PHB in relation to the main composition of the biomass (the content of proteins, polysaccharides, and lipids): after an incubation period of 7 days, a content of 6% of lipids (52% of which as unsaturated fatty acids with 18 carbon atoms), 12% of polysaccharides, 28% of proteins, and 46% of PHB was reached. 34536471 6 23 gly proteins 809:816 arg1 polysaccharides proteins polysaccharides Fterm proteins The cyanobacterium was then grown on a larger scale (10 L) to evaluate the characteristics of the produced PHB in relation to the main composition of the biomass (the content of proteins, polysaccharides, and lipids): after an incubation period of 7 days, a content of 6% of lipids (52% of which as unsaturated fatty acids with 18 carbon atoms), 12% of polysaccharides, 28% of proteins, and 46% of PHB was reached. 34536471 6 60 gly PHB 1029:1031 arg1 polysaccharides PHB polysaccharides OGER PHB P35232 The cyanobacterium was then grown on a larger scale (10 L) to evaluate the characteristics of the produced PHB in relation to the main composition of the biomass (the content of proteins, polysaccharides, and lipids): after an incubation period of 7 days, a content of 6% of lipids (52% of which as unsaturated fatty acids with 18 carbon atoms), 12% of polysaccharides, 28% of proteins, and 46% of PHB was reached. 33906120 8 13 gly glycoproteins 1271:1283 arg1 glycoproteins glycoproteins Fterm glycoproteins Analysis indicated that 57.66% of glycoproteins were highly conserved in other plant species while 42.34% of glycoproteins went unidentified among the conserved glycosylated homologous proteins in other plant species; these may be the new N-linked glycosylated proteins first identified in wheat. 33906120 8 29 gly glycoproteins 1346:1358 arg1 glycoproteins glycoproteins Fterm glycoproteins Analysis indicated that 57.66% of glycoproteins were highly conserved in other plant species while 42.34% of glycoproteins went unidentified among the conserved glycosylated homologous proteins in other plant species; these may be the new N-linked glycosylated proteins first identified in wheat. 33906120 8 43 gly glycosylated 1398:1409 arg1 the conserved glycosylated homologous proteins the conserved glycosylated homologous proteins Fterm proteins Analysis indicated that 57.66% of glycoproteins were highly conserved in other plant species while 42.34% of glycoproteins went unidentified among the conserved glycosylated homologous proteins in other plant species; these may be the new N-linked glycosylated proteins first identified in wheat. 33906120 8 65 gly glycosylated 1485:1496 arg1 the new N-linked glycosylated proteins the new N-linked glycosylated proteins Fterm proteins Analysis indicated that 57.66% of glycoproteins were highly conserved in other plant species while 42.34% of glycoproteins went unidentified among the conserved glycosylated homologous proteins in other plant species; these may be the new N-linked glycosylated proteins first identified in wheat. 33689141 9 47 gly residues 1671:1678 arg1 GPC GPC residues OGER GPC Q78HU7 The glycan residues on GPC provide an immune shield for the virus, and thus represent a target for the design and development of a vaccine. 32482891 9 67 part_of ACLP 1606:1609 arg1 the ACLP discoidin domain ACLP the ACLP discoidin domain PUBTATOR Site ACLP 165 domain We tested the hypothesis that a recently discovered 40-amino acid mutation and insertion in the ACLP discoidin domain regulates collagen binding and assembly. 33177197 2 78 gly glycosylated 295:306 arg1 hemagglutinin hemagglutinin Fterm hemagglutinin Both of hemagglutinin (HA) and NA are highly glycosylated proteins. 33177197 2 78 gly glycosylated 295:306 arg1 highly glycosylated proteins highly glycosylated proteins Fterm proteins Both of hemagglutinin (HA) and NA are highly glycosylated proteins. 32910214 9 48 gly multi-glycosylated 1260:1277 arg1 mono- and multi-glycosylated peptides mono- and multi-glycosylated peptides peptides Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample. 33232205 9 19 part_of S 1910:1910 arg1 bacterially-expressed S fragments S bacterially-expressed S fragments PUBTATOR Site S 43740568 fragments This study revealed that the native S glycoprotein stimulated neutralizing antibodies, while bacterially-expressed S fragments did not. 33292056 0 85 gly glycoprotein 102:113 arg1 SARS-CoV-2 spike glycoprotein spike glycoprotein carbohydrate-binding agents PUBTATOR spike glycoprotein 43740568 Sensing the interactions between carbohydrate-binding agents and N-linked glycans of SARS-CoV-2 spike glycoprotein using molecular docking and simulation studies. 33292056 0 85 gly glycoprotein 102:113 arg1 SARS-CoV-2 spike glycoprotein spike glycoprotein N-linked glycans PUBTATOR spike glycoprotein 43740568 Sensing the interactions between carbohydrate-binding agents and N-linked glycans of SARS-CoV-2 spike glycoprotein using molecular docking and simulation studies. 34215103 5 23 part_of sites 1231:1235 arg1 65 glycoproteins glycoproteins sites Fterm Site glycoproteins sites 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 34215103 5 67 part_of glycoproteins 1266:1278 arg1 125 N-glycosylation sites glycoproteins 125 N-glycosylation sites Fterm Site glycoproteins sites 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 34215103 5 67 part_of glycoproteins 1266:1278 arg1 134 glycopeptides glycoproteins 134 glycopeptides Fterm Site glycoproteins glycopeptides 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 33001090 2 0 gly glycosylation 317:329 arg1 the spike (S) protein the spike (S) protein OGER S) protein Q15517 Viral invasion through envelope proteins is modulated by N-linked glycosylation in the spike (S) protein. 32244903 5 57 gly glycoprotein 887:898 arg1 The glycoprotein The glycoprotein Fterm glycoprotein The glycoprotein we produced was recognized by both anti-B. 34500222 0 24 gly glycopeptides 106:118 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides Graphene functionalized with structurally complementary amino acids for sensitive recognition of N-linked glycopeptides. 33232205 5 11 gly glycosylated 1128:1139 arg1 The membrane (M) protein The membrane (M) protein Fterm protein The membrane (M) protein was partially N-linked glycosylated; the accessory protein 3a existed in three different forms, indicative of cleavage and dimerization. 34142147 5 3 gly sites 790:794 arg1 Sec31A Sec31A sites PUBTATOR Sec31A 22872 We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. 34142147 5 3 gly sites 790:794 arg1 Sec24D Sec24D sites PUBTATOR Sec24D 9871 We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. 34142147 5 3 gly sites 790:794 arg1 Sec24C Sec24C sites PUBTATOR Sec24C 9632 We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. 34490144 8 42 gly glycosylation 1198:1210 arg2 the glycosylation sites the glycosylation sites sites Although the glycosylation sites only constitute a minor subpopulation of the available epitopes, we observed a notable difference in the immunogenicity of the glycosylated YghJ and the non-glycosylated protein variant. 34490144 8 71 gly glycosylated 1345:1356 arg1 the glycosylated YghJ the glycosylated YghJ PUBTATOR YghJ 2847716 Although the glycosylation sites only constitute a minor subpopulation of the available epitopes, we observed a notable difference in the immunogenicity of the glycosylated YghJ and the non-glycosylated protein variant. 34490144 8 78 gly non-glycosylated 1371:1386 arg1 the non-glycosylated protein variant the non-glycosylated protein variant Fterm variant Although the glycosylation sites only constitute a minor subpopulation of the available epitopes, we observed a notable difference in the immunogenicity of the glycosylated YghJ and the non-glycosylated protein variant. 33728790 5 35 gly asparagine 1201:1210 arg1 asparagine - oligosaccharide linkage asparagine asparagine - oligosaccharide linkage asparagine Through PNGase F deglycosylation assays and site-directed mutagenesis of a GPI transamidase protein (GPI16) upregulated in cysts and a heat shock protein 70 (HSP70) upregulated in oospores, we demonstrated that both proteins were N-glycosylated and that the Nglyco -N motif is a target site for asparagine - oligosaccharide linkage. 33728790 5 42 gly N-glycosylated 1136:1149 arg1 both proteins both proteins Fterm proteins Through PNGase F deglycosylation assays and site-directed mutagenesis of a GPI transamidase protein (GPI16) upregulated in cysts and a heat shock protein 70 (HSP70) upregulated in oospores, we demonstrated that both proteins were N-glycosylated and that the Nglyco -N motif is a target site for asparagine - oligosaccharide linkage. 33673381 9 2 part_of δ-ENaC 951:956 arg1 carboxyl terminus domain -ENaC carboxyl terminus domain PUBTATOR Site -ENaC 6339 domain Neither N-linked glycosylation sites nor carboxyl terminus domain of δ-ENaC was necessary for the TNF-induced activation of whole-cell Na+ current in δ-ENaC KD of A549 cells. 33647064 6 63 part_of GPC 734:736 arg1 GPC N83 GPC GPC N83 PUBTATOR SpecificSite GPC P04921 N83 and N166 Interestingly, mutations resulting in acquisition of N-linked glycans at GPC N83 and N166 frequently occurred in late stages of the infection. 32331895 3 5 gly N-glycosylation 601:615 arg2 N-glycosylation sites N-glycosylation sites sites This study investigated the effect of mutagenesis of N-glycosylation sites on the transport function of bPepT2 in Chinese hamster ovary (CHO) cells. 33363554 0 3 gly Polysaccharide 9:22 arg1 Contrasting Climatic Regions Contrasting Climatic Regions Contrasting Climatic Regions Mucilage Polysaccharide Composition and Exudation in Maize From Contrasting Climatic Regions. 33363554 0 42 gly Regions 85:91 arg1 Polysaccharide Polysaccharide Mucilage Polysaccharide Composition and Exudation in Maize From Contrasting Climatic Regions. 31859482 1 19 gly glycoproteins 196:208 arg1 glycoproteins glycoproteins Fterm glycoproteins Aberrant glycosylation has been shown to associate with disease progression, and with glycoproteins representing the major protein component of biological fluids this makes them attractive targets for disease monitoring. 32012886 6 51 gly sialylated 903:912 arg1 PrPSc PrPSc PUBTATOR PrPSc 19122 The current work found that in 22L -infected animals, PrPSc is indeed sialylated in a region dependent manner. 32844720 3 4 part_of HDL 350:352 arg1 HDL protein composition HDL HDL protein composition OGER Site HDL Q9UNE0 position METHODS AND RESULTS Changes in HDL protein composition and function (cholesterol efflux capacity) in patients after acute ischemic stroke at 2 time points (24 hours, 35 patients; 96 hours, 20 patients) and in 35 control subjects were measured. 32241176 5 40 gly used 1040:1043 arg2 a GAG-binding peptide a GAG-binding peptide peptide Here, a GAG-binding peptide (GBP) with high affinity for HS and high cell-penetrating activity was used to develop an HS-targeting delivery system. 34030043 0 27 part_of TIGIT 31:35 arg1 TIGIT Asn32 TIGIT TIGIT Asn32 PUBTATOR AminoAcid TIGIT 201633 Asn32 and Asn101 The N-linked glycosylations of TIGIT Asn32 and Asn101 facilitate PVR/TIGIT interaction. 34934932 6 31 gly HBP 899:901 arg1 glucosamine HBP glucosamine OGER HBP P20160 Administration of glucosamine, a substrate of HBP, induces protein O-GlcNAcylation, Akt activation, and cardiomyocyte hypertrophy. 32892942 7 60 gly N-glycopeptides 957:971 arg2 N-glycopeptides N-glycopeptides N-glycopeptides The selectivity of the HMS towards N-glycopeptides had almost no decrease when the molar ratio of BSA tryptic digest to IgG tryptic digest was increased from 10:1 to 100:1. 34734627 1 38 gly glycosylation 118:130 arg1 proteins proteins Fterm proteins 1666 N-linked glycosylation of proteins entering the secretory pathway is an essential modification required for protein stability and function. 32885501 5 21 gly sialylated 806:815 arg1 multiple sialylated glycoforms multiple sialylated glycoforms The method effectively separates multiple sialylated glycoforms and offers a rapid detection of changes in glycosylation profile in 6 min. 33180459 3 51 gly glycosylation 808:820 arg2 both N-linked glycosylation sites both N-linked glycosylation sites sites Our results show that triantennary glycans can be sterically accommodated in-register on both N-linked glycosylation sites of each monomer. 33806920 4 5 gly hypoglycosylated 670:685 arg1 hypoglycosylated COX-2 hypoglycosylated COX-2 PUBTATOR COX-2 5743 Metabolic labeling experiments using 35S or cycloheximide suggested that the formation of hypoglycosylated COX-2 was dependent on de novo synthesis of the protein rather than the deglycosylation of the native protein. 33806920 4 48 gly deglycosylation 759:773 arg1 the native protein the native protein Fterm protein Metabolic labeling experiments using 35S or cycloheximide suggested that the formation of hypoglycosylated COX-2 was dependent on de novo synthesis of the protein rather than the deglycosylation of the native protein. 34590401 7 48 part_of FEN1 797:800 arg1 352 FEN1 352 PUBTATOR SpecificSite FEN1 2237 serine 352 O-GlcNAcylation at the serine 352 of FEN1 disrupts its interaction with Proliferating Cell Nuclear Antigen (PCNA) at the replication foci, and leads to altered cell cycle, defects in DNA replication, accumulation of DNA damage, and enhanced sensitivity to DNA damage agents. 32439055 1 27 gly glycopeptides 249:261 arg2 the glycopeptides enrichment efficiency the glycopeptides enrichment efficiency glycopeptides A hydrophilic two-dimensional titanium-based metal-organic framework (MOF) nanosheets were prepared and applied to improve the glycopeptides enrichment efficiency. 34590401 1 32 gly proteins 220:227 arg1 O-linked N-acetylglucosamine proteins O-linked N-acetylglucosamine Fterm proteins O-linked N-acetylglucosamine (O-GlcNAcylation) is a ubiquitous post-translational modification of proteins that is essential for cell function. 33270417 9 16 gly glycosylation 1391:1403 arg1 a therapeutic peptide peptide peptide At last, N-linked glycosylation on INGAP-P, a therapeutic peptide, increased the deamidation half-life of INGAP-P as well as its therapeutic potency. 34020366 1 50 gly used 234:237 arg2 Egg white peptides Egg white peptides peptides Egg white peptides (EWPs) can be effectively used to alleviate and treat inflammatory diseases due to their anti-oxidation, anti-inflammation, and microbiota regulation capabilities. 32441096 7 0 gly arm-fucosylated 1321:1335 arg1 outer arm-fucosylated glycans outer arm-fucosylated glycans Initial results in liver and prostate tissues indicate core-fucosylated glycans associated to specific tissue regions while still demonstrating a diverse mix of core- and outer arm-fucosylated glycans throughout all regions of tissue. 32441096 7 10 gly core-fucosylated 1199:1214 arg1 core-fucosylated glycans core-fucosylated glycans Initial results in liver and prostate tissues indicate core-fucosylated glycans associated to specific tissue regions while still demonstrating a diverse mix of core- and outer arm-fucosylated glycans throughout all regions of tissue. 34649588 1 15 gly glycoproteins 145:157 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins BACKGROUND The production of N-linked glycoproteins in genetically amenable bacterial hosts offers great potential for reduced cost, faster/simpler bioprocesses, greater customisation, and utility for distributed manufacturing of glycoconjugate vaccines and glycoprotein therapeutics. 34649588 1 81 gly glycoprotein 365:376 arg1 glycoprotein therapeutics glycoprotein therapeutics Fterm glycoprotein BACKGROUND The production of N-linked glycoproteins in genetically amenable bacterial hosts offers great potential for reduced cost, faster/simpler bioprocesses, greater customisation, and utility for distributed manufacturing of glycoconjugate vaccines and glycoprotein therapeutics. 34260942 4 12 gly 111 717:719 arg1 O-GlcNAc modification serine 111 O-GlcNAc modification serine 111 Mechanistically, O-GlcNAc modification of serine 111 (S111) of Psme3 promotes degradation of Ddx6, which is essential for processing body (P-body) assembly, resulting in the maintenance of ESC pluripotent state. 34260942 4 44 gly modification 694:705 arg3 serine 111 AND O-GlcNAc modification serine 111 O-GlcNAc modification serine 111 Mechanistically, O-GlcNAc modification of serine 111 (S111) of Psme3 promotes degradation of Ddx6, which is essential for processing body (P-body) assembly, resulting in the maintenance of ESC pluripotent state. 34284575 6 35 gly glycopeptides 821:833 arg2 O-linked glycopeptides O-linked glycopeptides glycopeptides Herein, we designed the biomimetic materials, Trp-Arg (WR) monomer-grafted silica microspheres (designated as WR-SiO2), and these biomimetic materials can enrich N- and O-linked glycopeptides with high selectivity. 34559939 4 39 gly α2,6-sialyltransferase 635:656 arg1 ST6Gal-I α2,6-sialyltransferase I ST6Gal-I Cterm α2,6-sialyltransferase I 170589 The silencing of α2,6-sialyltransferase I (ST6Gal-I) significantly reduced the levels of α2,6-sialylated glycoconjugates on CD63 and the surface of HCC-derived exosomes (HCC-exo). 32846088 5 2 part_of receptor-binding 637:652 arg1 their receptor-binding domains receptor their receptor-binding domains Fterm Site receptor domains Although the surface domains of both spike proteins are highly similar, their receptor-binding domains (RBDs) and the O-linked glycan domains are structurally different. 32846088 5 4 part_of proteins 602:609 arg1 the surface domains spike proteins the surface domains PUBTATOR Site spike proteins 43740568 domains Although the surface domains of both spike proteins are highly similar, their receptor-binding domains (RBDs) and the O-linked glycan domains are structurally different. 34711323 2 65 gly Glycosylation 201:213 arg1 EPO EPO PUBTATOR EPO 2056 Glycosylation of EPO influences its safety and efficacy and is defined as a critical quality attribute. 34455356 0 55 gly glycosylation 33:45 arg1 duck Tembusu virus NS1 NS1 sites OGER NS1 Q9Y6Y0 sites N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 55 gly glycosylation 33:45 arg1 duck Tembusu virus NS1 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 55 gly glycosylation 33:45 arg2 N130 N130, N175 and N207 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 55 gly glycosylation 33:45 arg2 N175 N130, N175 and N207 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 55 gly glycosylation 33:45 arg2 N130 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 55 gly glycosylation 33:45 arg2 N175 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 55 gly glycosylation 33:45 arg2 N175 N130, N175 and N207 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 32910214 8 33 part_of PNGase-treated 1146:1159 arg1 the enriched and PNGase-treated deglycosylated peptides PNGase the enriched and PNGase-treated deglycosylated peptides PUBTATOR Site PNGase 55768 peptides Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. 34622651 5 49 gly glycopeptides 661:673 arg2 intact glycopeptides intact glycopeptides glycopeptides However, substantial challenges are still present in the quantitative analysis of intact glycopeptides with high accuracy at high throughput. 33863420 4 42 gly glycopeptides 1008:1020 arg2 24 glycopeptides α-casein glycopeptides Fterm α-casein glycopeptides By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 33863420 4 42 gly glycopeptides 1008:1020 arg1 IgG IgG glycopeptides Cterm IgG glycopeptides By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 33647064 7 23 gly glycosylation 810:822 arg2 These glycosylation sites These glycosylation sites sites These glycosylation sites are conserved in the GPC of wild-type MACV, indicating that this is a phenotypic reversion for the chimeric MACV to gain those glycans crucial for infection in vivo. 32609386 4 46 part_of HIV 519:521 arg1 HIV gp120 sequences HIV gp120 HIV gp120 sequences PUBTATOR Site HIV gp120 3700 sequences HIV gp120 sequences from antiretroviral naïve patients were analyzed for V2 tripeptide composition, length, net charge, and potential N-linked-glycosylation sites. 32609386 4 66 part_of gp120 523:527 arg1 HIV gp120 sequences HIV gp120 HIV gp120 sequences PUBTATOR Site HIV gp120 3700 sequences HIV gp120 sequences from antiretroviral naïve patients were analyzed for V2 tripeptide composition, length, net charge, and potential N-linked-glycosylation sites. 33023909 8 20 gly Thr626 1727:1732 arg1 O-GlcNAcylation Thr626 O-GlcNAcylation Thr626 Decrease in SOCE activity due to increase and decrease in O-GlcNAcylation was attributable to reduced phosphorylation at Ser621 These data suggest that both decrease in O-GlcNAcylation at Thr626 and increase in O-GlcNAcylation at Ser621 in STIM1 lead to impairment of SOCE activity through decrease in Ser621 phosphorylation. 33023909 8 35 gly Ser621 1769:1774 arg1 O-GlcNAcylation Ser621 O-GlcNAcylation Ser621 Decrease in SOCE activity due to increase and decrease in O-GlcNAcylation was attributable to reduced phosphorylation at Ser621 These data suggest that both decrease in O-GlcNAcylation at Thr626 and increase in O-GlcNAcylation at Ser621 in STIM1 lead to impairment of SOCE activity through decrease in Ser621 phosphorylation. 34327998 5 13 gly glycopeptide 792:803 arg2 these reference glycopeptide abundance distribution spectra these reference glycopeptide abundance distribution spectra glycopeptide Likewise, these reference glycopeptide abundance distribution spectra (GADS) can be stored in searchable libraries. 33710212 6 49 gly glycosylation 817:829 arg2 A C. jejuni glycosylation motif A C. jejuni glycosylation motif motif A C. jejuni glycosylation motif is genetically incorporated to tasA gene and expressed in Escherichia coli containing the C. jejuni pgl protein glycosylation pathway. 32518939 3 1 part_of database 528:535 arg1 an EGF-LD sequence database an EGF-LD sequence Fterm Site database sequence We searched in KEGG GENES database to list mouse and human proteins with an EGF-LD sequence including one, two, three or four potential O-glycosylation consensus sites. 32518939 3 60 part_of EGF-LD 578:583 arg1 an EGF-LD sequence EGF an EGF-LD sequence OGER Site EGF P01132 sequence We searched in KEGG GENES database to list mouse and human proteins with an EGF-LD sequence including one, two, three or four potential O-glycosylation consensus sites. 34636569 1 14 gly glycopeptide 369:380 arg2 intact glycopeptide determination intact glycopeptide determination glycopeptide N-linked glycosylation plays important roles in multiple physiological and pathological processes, while the analysis coverage is still limited due to the insufficient digestion of glycoproteins, as well as incomplete ion fragments for intact glycopeptide determination. 34636569 1 34 gly glycoproteins 307:319 arg1 glycoproteins glycoproteins Fterm glycoproteins N-linked glycosylation plays important roles in multiple physiological and pathological processes, while the analysis coverage is still limited due to the insufficient digestion of glycoproteins, as well as incomplete ion fragments for intact glycopeptide determination. 34069226 2 66 gly contains 230:237 arg1 The Fc domain AND an N-linked oligosaccharide The Fc domain an N-linked oligosaccharide domain The Fc domain of IgG antibodies contains an N-linked oligosaccharide at position 297. 34069226 2 66 gly contains 230:237 arg1 position 297 AND an N-linked oligosaccharide The Fc domain position 297 an N-linked oligosaccharide position 297 The Fc domain of IgG antibodies contains an N-linked oligosaccharide at position 297. 32878894 3 73 part_of gp85 965:968 arg1 C terminus gp85 C terminus Cterm Site gp85 terminus Our results showed that hemagglutinin (HA) substitutions of amino acids (aa) 38 to 131 (N terminus of gp85) and aa 159 to 283 (C terminus of gp85) significantly inhibited the interaction between gp85 and chNHE1/chNHE1 loop 1. 32878894 3 108 part_of gp85 926:929 arg1 N terminus gp85 N terminus Cterm Site gp85 terminus Our results showed that hemagglutinin (HA) substitutions of amino acids (aa) 38 to 131 (N terminus of gp85) and aa 159 to 283 (C terminus of gp85) significantly inhibited the interaction between gp85 and chNHE1/chNHE1 loop 1. 34056367 12 72 gly glycosylated 2177:2188 arg1 small or large membrane proteins small or large membrane proteins Fterm proteins Our experiments demonstrated that small or large membrane proteins that contain an N-glycosylation consensus sequence can be glycosylated by NGT in membrane-mimetic environments. 34155551 0 84 gly glycopeptides 61:73 arg2 neutral and sialyl glycopeptides neutral and sialyl glycopeptides glycopeptides Simultaneous enrichment and separation of neutral and sialyl glycopeptides of SARS-CoV-2 spike protein enabled by dual-functionalized Ti-IMAC material. 32117864 4 12 gly sialoglycosites 880:894 arg2 "sialoglycosites "sialoglycosites sialoglycosites We then analyzed N-glycan sialylation using solid phase extraction of glycopeptides (SPEG) mass spectrometry-based proteomics under conditions that selectively captured sialic acid-containing glycopeptides, referred to as "sialoglycosites." 32117864 4 37 gly glycopeptides 727:739 arg2 glycopeptides glycopeptides glycopeptides We then analyzed N-glycan sialylation using solid phase extraction of glycopeptides (SPEG) mass spectrometry-based proteomics under conditions that selectively captured sialic acid-containing glycopeptides, referred to as "sialoglycosites." 32120884 3 17 gly glycoproteins 687:699 arg1 Jingmen flavi-like virus glycoproteins Jingmen flavi-like virus glycoproteins Fterm glycoproteins Jingmen flavi-like virus glycoproteins have a sequence enriched in serine, threonine, and proline at the amino terminus, which is a feature of mucin-like domains. 33689141 3 49 gly chain 591:595 arg1 GPC GPC chain OGER GPC Q78HU7 We used three mutagenesis strategies (asparagine to glutamine, asparagine to alanine, and serine/tyrosine to alanine mutants) to abolish individual glycan chain on GPC and found that all the three strategies led to cleavage inefficiency on the 2nd (N89), 5th (N119), or 8th (N365) glycosylation motif. 33689141 3 72 gly glycosylation 717:729 arg2 8th (N365) glycosylation motif 8th (N365) glycosylation motif motif We used three mutagenesis strategies (asparagine to glutamine, asparagine to alanine, and serine/tyrosine to alanine mutants) to abolish individual glycan chain on GPC and found that all the three strategies led to cleavage inefficiency on the 2nd (N89), 5th (N119), or 8th (N365) glycosylation motif. 33024038 2 102 gly glycosylation 423:435 arg2 their glycosylation site their glycosylation site site However, pathogenic, and commensal bacteria have evolved specific glycoproteases that overcome the steric impediment posed by carbohydrates, cleaving glycoproteins precisely at their glycosylation site(s). 33024038 2 112 gly glycoproteins 390:402 arg1 glycoproteins glycoproteins Fterm glycoproteins However, pathogenic, and commensal bacteria have evolved specific glycoproteases that overcome the steric impediment posed by carbohydrates, cleaving glycoproteins precisely at their glycosylation site(s). 33914740 5 56 gly glycosylation 950:962 arg2 Ser19 Ser19 Ser19 We identified that the glycosylation occurs at Ser96, Ser101 and Ser110 on the large chain and Ser19 on the small polypeptide chain of rBer e 1 only. 33914740 5 56 gly glycosylation 950:962 arg2 Ser101 Ser96, Ser101 and Ser110 Ser96, Ser101 and Ser110 We identified that the glycosylation occurs at Ser96, Ser101 and Ser110 on the large chain and Ser19 on the small polypeptide chain of rBer e 1 only. 33914740 5 56 gly glycosylation 950:962 arg2 Ser110 Ser96, Ser101 and Ser110 Ser96, Ser101 and Ser110 We identified that the glycosylation occurs at Ser96, Ser101 and Ser110 on the large chain and Ser19 on the small polypeptide chain of rBer e 1 only. 33914740 5 56 gly glycosylation 950:962 arg2 Ser110 Ser96, Ser101 and Ser110 Ser96, Ser101 and Ser110 We identified that the glycosylation occurs at Ser96, Ser101 and Ser110 on the large chain and Ser19 on the small polypeptide chain of rBer e 1 only. 33346724 5 6 gly glycoprotein 672:683 arg1 the N-linked glycoprotein glycans the N-linked glycoprotein glycans We investigated whether accumulated low doses of IR (LoDIR) affect the N-linked glycoprotein glycans using Medaka fish (Oryzias latipes). 31724933 3 38 gly glycosylation 332:344 arg2 N-linked glycosylation sites N-linked glycosylation sites sites Glycoprotein RGD motifs and N-linked glycosylation sites have been predicted to mediate receptor binding or play important roles in virus entry into host cells, yet their function needs to be validated. 34649588 7 90 gly glycoprotein 1481:1492 arg1 glycoprotein glycoprotein Fterm glycoprotein Furthermore, we also demonstrate that supplementation with the chemical oxidant cystine enhances the titre of glycoprotein in an oxidoreductase knockout strain by improving total protein production and cell fitness, while at the same time maintaining higher levels of glycosylation efficiency. 33997889 5 32 gly glycosylates 844:855 arg1 ComP ComP OGER ComP P49747 PglS is a pilin-specific O-linking OTase that naturally glycosylates a single protein, ComP. 33997889 5 32 gly glycosylates 844:855 arg1 a single protein a single protein Fterm protein PglS is a pilin-specific O-linking OTase that naturally glycosylates a single protein, ComP. 32897971 0 1 gly Epitope 44:50 arg1 O-GlcNAcH O-GlcNAcH The O-Linked N-Acetylglucosamine Containing Epitope H (O-GlcNAcH) is Upregulated in the Trophoblastic and Downregulated in the Fibroblastic Cells in Missed Miscarriage Human Chorionic Villi With Simple Hydropic Degeneration. 35386843 4 51 gly Glycans 509:515 arg1 glycopeptides glycopeptides glycopeptides Glycans and glycoconjugates (i.e., glycopeptides, nucleotide sugars, etc.) in non-volatile buffer (e.g., Tris buffer) can be loaded on the paper substrate from which buffers can be removed by washing with ACN/H2O (90/10 v/v) solution. 35386843 4 69 gly glycopeptides 544:556 arg2 glycopeptides glycopeptides glycopeptides Glycans and glycoconjugates (i.e., glycopeptides, nucleotide sugars, etc.) in non-volatile buffer (e.g., Tris buffer) can be loaded on the paper substrate from which buffers can be removed by washing with ACN/H2O (90/10 v/v) solution. 34722332 2 52 part_of gut 394:396 arg1 gut microbiota composition gut gut microbiota composition PUBTATOR Site gut 110006 position, However, the interaction between chronic restraint stress (CRS), gut microbiota composition, and colonic mucus remains unclear. 34201710 1 37 part_of protein 247:253 arg1 the cell wall protein composition protein the cell wall protein composition Fterm Site protein position High temperature stress leads to complex changes to plant functionality, which affects, i.a., the cell wall structure and the cell wall protein composition. 33409271 5 6 part_of interferon 800:809 arg1 an N-terminal interferon alpha 2 (IFNα2) signal peptide N-terminal interferon alpha 2 an N-terminal interferon alpha 2 (IFNα2) signal peptide PUBTATOR Site N-terminal interferon alpha 2 3440 peptide The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 48 part_of N-terminal 789:798 arg1 an N-terminal interferon alpha 2 (IFNα2) signal peptide N-terminal interferon alpha 2 an N-terminal interferon alpha 2 (IFNα2) signal peptide PUBTATOR Site N-terminal interferon alpha 2 3440 peptide The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 65 part_of alpha 811:815 arg1 an N-terminal interferon alpha 2 (IFNα2) signal peptide N-terminal interferon alpha 2 an N-terminal interferon alpha 2 (IFNα2) signal peptide PUBTATOR Site N-terminal interferon alpha 2 3440 peptide The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 70 part_of protein 949:955 arg1 a human rhinovirus 3C protease (HRV3C) cleavage site 2019-nCoV spike protein a human rhinovirus 3C protease (HRV3C) cleavage site PUBTATOR Site 2019-nCoV spike protein 43740568 site The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 70 part_of protein 949:955 arg1 an N-terminal interferon alpha 2 (IFNα2) signal peptide 2019-nCoV spike protein an N-terminal interferon alpha 2 (IFNα2) signal peptide PUBTATOR Site 2019-nCoV spike protein 43740568 peptide The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 105 part_of protease 886:893 arg1 a human rhinovirus 3C protease (HRV3C) cleavage site protease a human rhinovirus 3C protease (HRV3C) cleavage site Fterm Site protease site The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33536395 1 30 gly glycosylation 276:288 arg1 N-linked oligosaccharides N-linked oligosaccharides The gmn2 mutant of Schizosaccharomyces pombe has previously been shown to exhibit defects in protein glycosylation of N-linked oligosaccharides (Ballou, L. and Ballou, CE., Proc. 34725712 5 60 gly glycosites 779:788 arg2 The glycoproteome and glycosites The glycoproteome and glycosites glycosites The glycoproteome and glycosites modified by four OGT TPR isoforms were examined on the whole proteome and a single target protein, GFP-JunB. 33673381 9 15 gly glycosylation 899:911 arg1 sites sites sites Neither N-linked glycosylation sites nor carboxyl terminus domain of δ-ENaC was necessary for the TNF-induced activation of whole-cell Na+ current in δ-ENaC KD of A549 cells. 33868212 0 49 part_of Small 8:12 arg1 LCG-N25 Small LCG-N25 OGER SpecificSite Small N25 A Novel Small Molecule, LCG-N25, Inhibits Oral Streptococcal Biofilm. 32878894 6 20 gly glycosylation 1633:1645 arg2 two N-linked glycosylation sites two N-linked glycosylation sites sites Taken together, our findings indicated that the bipartite sequence motif, spanning aa 38 to 131 and aa 159 to 283, of ALV-J gp85 was essential for binding to chNHE1, with its two N-linked glycosylation sites and two cysteines being important for its receptor-binding function and subsequent viral infection steps.IMPORTANCE Infection of a cell by retroviruses requires the attachment and fusion of the host and viral membranes. 32046000 5 27 gly glycopeptide 924:935 arg2 sufficient glycopeptide sufficient glycopeptide glycopeptide Following optimization of SgtA GT activity, we generated sufficient glycopeptide (10 mg) to allow conjugation to KLH (keyhole limpet hemocyanin) protein. 32482891 6 1 gly glycosylation 1015:1027 arg1 Asn-471 Asn-471 and Asn-1030 Asn-471 and Asn-1030 Using site-directed mutagenesis, we determined that glycosylation of Asn-471 and Asn-1030 is necessary for ACLP secretion and identified a specific N-terminal proteolytic ACLP fragment. 34649588 10 93 gly N-glycoprotein 2278:2291 arg1 heterologous recombinant N-glycoprotein production heterologous recombinant N-glycoprotein production Fterm N-glycoprotein The process and genetic strategies identified herein will inform further optimisation and scale-up of heterologous recombinant N-glycoprotein production. 32887230 0 5 part_of Differences 4:14 arg1 Intestinal Microbial Composition Sex Differences Intestinal Microbial Composition OGER Site Sex Differences P51805 position Sex Differences in Intestinal Microbial Composition and Function of Hainan Special Wild Boar. 34342946 0 42 gly glycoprotein 123:134 arg1 human myelin oligodendrocyte glycoprotein human myelin oligodendrocyte glycoprotein PUBTATOR myelin oligodendrocyte glycoprotein 4340 The extracellular region of bovine milk butyrophilin exhibits closer structural similarity to human myelin oligodendrocyte glycoprotein than to immunological BTN family receptors. 32996013 3 74 gly glycosylation 332:344 arg1 serine/threonine residues proteins residues Fterm proteins residues O-GlcNAcylation, a dynamic and ubiquitous post-translational glycosylation of proteins on serine/threonine residues, has been usually considered as a nutrient sensor. 32996013 3 84 gly glycosylation 332:344 arg1 serine/threonine residues proteins residues Fterm proteins residues O-GlcNAcylation, a dynamic and ubiquitous post-translational glycosylation of proteins on serine/threonine residues, has been usually considered as a nutrient sensor. 32996013 3 84 gly glycosylation 332:344 arg1 serine/threonine residues residues residues O-GlcNAcylation, a dynamic and ubiquitous post-translational glycosylation of proteins on serine/threonine residues, has been usually considered as a nutrient sensor. 33182731 3 8 gly glycoproteins 664:676 arg1 SPEG SPEG PUBTATOR SPEG Q15772 To overcome this pitfall, in this report we compared three glycoproteomic analysis methods; first the recently developed N-linked glycans and glycosite-containing peptides (NGAG) chemoenzymatic method, second, solid-phase extraction of N-linked glycoproteins (SPEG), and third, hydrophilic interaction liquid chromatography (HILIC) by characterizing N-linked glycosites in the secretome of Chinese hamster ovary (CHO) cells. 33182731 3 8 gly glycoproteins 664:676 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins To overcome this pitfall, in this report we compared three glycoproteomic analysis methods; first the recently developed N-linked glycans and glycosite-containing peptides (NGAG) chemoenzymatic method, second, solid-phase extraction of N-linked glycoproteins (SPEG), and third, hydrophilic interaction liquid chromatography (HILIC) by characterizing N-linked glycosites in the secretome of Chinese hamster ovary (CHO) cells. 33182731 3 10 gly glycosites 778:787 arg2 N-linked glycosites N-linked glycosites glycosites To overcome this pitfall, in this report we compared three glycoproteomic analysis methods; first the recently developed N-linked glycans and glycosite-containing peptides (NGAG) chemoenzymatic method, second, solid-phase extraction of N-linked glycoproteins (SPEG), and third, hydrophilic interaction liquid chromatography (HILIC) by characterizing N-linked glycosites in the secretome of Chinese hamster ovary (CHO) cells. 33755116 0 31 gly glycoprotein 83:94 arg1 HIV-1 Env glycoprotein HIV-1 Env glycoprotein PUBTATOR HIV-1 Env glycoprotein 100616444 Development of Martini 2.2 parameters for N-glycans: a case study of the HIV-1 Env glycoprotein dynamics. 32337418 6 14 gly N-glycoforms 1194:1205 arg1 subclass-specific IgG Fc N-glycoforms subclass-specific IgG Fc N-glycoforms PUBTATOR IgG 16059 The method was applied to quantify the differences in subclass-specific IgG Fc N-glycoforms between systemic sclerosis (SSc) mice and healthy control mice. 32633815 6 28 gly domain 902:907 arg1 O-linked glycan domain O-linked glycan domain The key residues of the receptor-binding domain (RBD) and O-linked glycan were compared. 34322281 13 61 gly used 2083:2086 arg2 the key sequence the key sequence sequence We anticipate that the key sequence and structural motifs can be extended to analyze specificities of other isoforms of the GalNAc-T family and can be used to guide design of variants with tailored specificity. 34215103 3 101 gly glycopeptides 745:757 arg2 endogenous glycopeptides endogenous glycopeptides glycopeptides Herein, we reported a novel bowl-like mesoporous polydopamine nanoparticle modified by carrageenan (denoted as MPDA@PEI@CA) with strong hydrophilicity and size-exclusion effect for high specificity enrichment of endogenous glycopeptides. 32801002 4 39 part_of OGT 673:675 arg1 the OGT binding site OGT the OGT binding site PUBTATOR Site OGT 8473 site Molecular modeling study of selected inhibitors into the OGT binding site provided insight into the behavior by which these compounds interact with the enzyme. 34520755 1 21 gly N-glycosylation 220:234 arg2 six potential N-glycosylation sites six potential N-glycosylation sites sites rHuPH20, a neutral pH-active hyaluronidase that degrades glycosaminoglycans under physiologic conditions, has six potential N-glycosylation sites. 34638625 1 9 part_of proteins 264:271 arg1 Ser and Thr residues proteins Ser and Thr residues Fterm AminoAcid proteins Ser and Thr residues Glycosyltransferase OGT catalyzes the conjugation of O-linked β-D-N-acetylglucosamine (O-GlcNAc) to Ser and Thr residues of the cellular proteins and regulates many key processes in the cell. 32352119 0 27 gly glycosylation 166:178 arg1 cellular nucleotide-sugar levels cellular nucleotide-sugar levels Robustness in glycosylation systems: effect of modified monosaccharides, acceptor decoys and azido sugars on cellular nucleotide-sugar levels and pattern of N-linked glycosylation. 34188486 1 16 gly glycosylated 237:248 arg1 a glycosylated protein a glycosylated protein Fterm protein CenA is an endoglucanase secreted by the Gram-positive cellulolytic bacterium, Cellulomonas fimi, to the environment as a glycosylated protein. 33316563 0 35 gly glycopeptides 134:146 arg2 endogenous glycopeptides endogenous glycopeptides glycopeptides Melamine foam assisted in-tip packed amine-functionalized titanium metal-organic framework for the selective enrichment of endogenous glycopeptides. 33889548 7 44 gly glycopeptides 1266:1278 arg2 Totally 195 glycopeptides Totally 195 glycopeptides glycopeptides Totally 195 glycopeptides were identified using a dedicated search engine pGlyco. 32878894 2 88 part_of ALV-J 676:680 arg1 the chNHE1-binding domain ALV-J gp85 the chNHE1-binding domain Cterm Site ALV-J gp85 domain In this study, to further clarify the precise organization and properties of the interaction between ALV-J gp85 and chNHE1, we identified the chNHE1-binding domain of ALV-J gp85 using a series of gp85 mutants with segment substitutions and evaluating their effects on chNHE1 binding in protein-cell binding assays. 33442735 1 48 part_of proteins 223:230 arg1 the serine/threonine residues proteins the serine/threonine residues Fterm Site proteins residues O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification (i.e., O-GlcNAcylation) on the serine/threonine residues of proteins. 31859482 0 3 gly Glycoproteins 65:77 arg1 Glycans Glycoproteins Glycans Fterm Glycoproteins Glycans, Glycosite, and Intact Glycopeptide Analysis of N-Linked Glycoproteins Using Liquid Handling Systems. 34555499 3 59 gly released 479:486 arg2 acidic glycoproteins AND Desialylated sulfated oligosaccharides acidic glycoproteins Desialylated sulfated oligosaccharides Fterm glycoproteins 3958 Desialylated sulfated oligosaccharides were released from acidic glycoproteins in the cyst fluid from one patient with a benign serous cyst and one patient with serous OC. 34555499 3 78 gly Desialylated 435:446 arg1 Desialylated sulfated oligosaccharides Desialylated sulfated oligosaccharides Desialylated sulfated oligosaccharides were released from acidic glycoproteins in the cyst fluid from one patient with a benign serous cyst and one patient with serous OC. 34555499 3 93 gly glycoproteins 500:512 arg1 acidic glycoproteins acidic glycoproteins Fterm glycoproteins 3958 Desialylated sulfated oligosaccharides were released from acidic glycoproteins in the cyst fluid from one patient with a benign serous cyst and one patient with serous OC. 34227365 25 12 gly N-glycoproteins 4057:4071 arg1 Differentially expressed N-glycoproteins Differentially expressed N-glycoproteins Fterm N-glycoproteins Differentially expressed N-glycoproteins were mostly associated with platelet degranulation, extracellular region, and ossification. 31463903 4 3 part_of ADAMTS7 685:691 arg1 the various ADAMTS7 domains ADAMTS7 the various ADAMTS7 domains PUBTATOR Site ADAMTS7 11173 domains For the benefit of scientists who study the function of ADAMTS7 and its role in disease, this chapter provides an introduction to the chemical and functional properties of the various ADAMTS7 domains, as well as a protocol for the recombinant expression and purification of ADAMTS7. 33284103 5 9 gly O-glycosylated 738:751 arg1 the residue the residue residue Remarkably, in human OCN (hOCN), the residue corresponding to S8 is a tyrosine (Y12), which is not O-glycosylated. 34069226 7 5 gly Afucosylated 928:939 arg1 Afucosylated human IgG1 Afucosylated human IgG1 OGER IgG1 P01857 Afucosylated human IgG1 showed stronger interaction with the murine FcγRIV, the mouse orthologue of human FcγRIIIa, indicating that this glycan change is functionally conserved between the species. 32306311 1 9 gly glycoproteins 226:238 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins Animal leguminous-type (L-type) lectins, including ERGIC-53 and VIP36 are responsible for intracellular transport and quality control of N-linked glycoproteins in the early secretory pathway. 34227365 6 22 gly N-glycoproteins 995:1009 arg1 urinary N-glycoproteins urinary N-glycoproteins Fterm N-glycoproteins Currently, there is a lack of specialized research on individual differences, physiological fluctuations, and physiological abundance ranges of urinary N-glycoproteins in large healthy populations. 32510947 2 32 gly glycosite 617:625 arg2 glycosite localization glycosite localization glycosite This is due in part to cleavage of the glycosidic bond occurring prior to the peptide backbone during collisionally activated dissociation (CAD), which leads to generation of characteristic oxocarbenium ions and impairs glycosite localization. 34071949 6 44 gly MDC1 1080:1083 arg1 O-GlcNAcylation MDC1 O-GlcNAcylation PUBTATOR MDC1 9656 We confirmed that O-GlcNAcylation of MDC1 is increased upon irradiation and identified additional repair factors related to Homologous Recombination (HR), CtIP and BRCA1, which were increasingly O-GlcNAcyated upon irradiation. 32501708 6 28 gly glycopeptides 770:782 arg2 glycopeptides glycopeptides glycopeptides High-definition differential IMS (FAIMS) had robustly disentangled isomeric peptides involving other PTMs but was not evaluated for glycopeptides that featured multilevel isomerism. 33335573 1 18 gly glycosylation 222:234 arg2 three potential N-linked glycosylation sites three potential N-linked glycosylation sites sites The hepatitis E virus (HEV) capsid protein pORF2 comprises three potential N-linked glycosylation sites. 34544801 0 43 part_of IgG 35:37 arg1 IgG V Regions IgG V Regions IgG V Regions Cterm IgG V Regions Elevated N-Linked Glycosylation of IgG V Regions in Myasthenia Gravis Disease Subtypes. 34544801 0 82 part_of V 39:39 arg1 IgG V Regions IgG V Regions IgG V Regions Cterm IgG V Regions Elevated N-Linked Glycosylation of IgG V Regions in Myasthenia Gravis Disease Subtypes. 34738170 6 7 part_of GPI-anchored 1130:1141 arg1 the GPI-anchored region GPI the GPI-anchored region OGER Site GPI P06744 region Mutational analysis of Pwp1p revealed that the GPI-anchored region of Pwp1p is important in attenuating the growth defect. 31869211 4 19 gly glycopeptides 600:612 arg2 glycopeptides glycopeptides glycopeptides Enrichment of glycopeptides from trypsin digests of immunoglobulin G (IgG) standard samples and of IgG isolated from real human plasma samples was accomplished via hydrophilic interaction liquid chromatography processes using ZIF-L-Co-S-Au-Cys. 32878894 0 106 part_of gp85 55:58 arg1 the N and C Termini gp85 the N and C Termini Cterm gp85 The Bipartite Sequence Motif in the N and C Termini of gp85 of Subgroup J Avian Leukosis Virus Plays a Crucial Role in Receptor Binding and Viral Entry. 34923442 10 15 gly proteins 1948:1955 arg1 polysaccharides proteins polysaccharides Fterm proteins The least amounts of proteins, polysaccharides and total suspended solids (TSS) on the surface of tested #M2 also demonstrated its outstanding biofouling resistance. 33073165 2 13 gly glycosylated 420:431 arg1 glycosylated 5-hydroxylysine 5-hydroxylysine (Hyl OGER 5-hydroxylysine (Hyl P42679 To fully investigative collagen biochemistry, one needs to assemble peptides that possess glycosylated 5-hydroxylysine (Hyl). 34562451 2 74 gly glycosylated 303:314 arg1 the glycosylated residues the glycosylated residues residues Two of the glycosylated residues are located in the scavenger receptor cysteine-rich (SRCR) protein domain, while the remaining two sites are in the catalytic serine protease (SP) domain. 32119511 7 13 gly glycosite 1256:1264 arg2 a similar glycosite profile a similar glycosite profile glycosite Glycoproteomics revealed that nanobody-OGT(4) or full-length OGT produced a similar glycosite profile on the target protein JunB and Nup62. 32157140 10 45 gly N-glycosylated 1312:1325 arg1 human GPR176 human GPR176 PUBTATOR GPR176 11245 We also demonstrate that human GPR176 is N-glycosylated. 34646892 0 104 gly c-Myc 36:40 arg1 Inhibiting Hyper-O-GlcNAcylation c-Myc Inhibiting Hyper-O-GlcNAcylation PUBTATOR c-Myc 4609 Inhibiting Hyper-O-GlcNAcylation of c-Myc accelerate diabetic wound healing by alleviating keratinocyte dysfunction. 32119511 10 29 gly glycosylation 1688:1700 arg1 desired proteins desired proteins Fterm proteins The use of a nanobody to redirect OGT substrate selection for glycosylation of desired proteins in cells may further constitute a generalizable strategy for controlling a broader array of post-translational modifications in cells. 33921866 8 9 gly proliferator 1438:1449 arg1 Fructose-induced upregulation peroxisome proliferator activated receptor α Fructose-induced upregulation PUBTATOR peroxisome proliferator activated receptor α 25747 Fructose-induced upregulation of complex II-driven mitochondrial respiration, peroxisome proliferator-activated receptor-gamma coactivator 1 alpha, and peroxisome proliferator activated receptor α also persisted after switching to control diet. 33921866 8 63 gly alpha 1416:1420 arg1 Fructose-induced upregulation peroxisome proliferator-activated receptor-gamma coactivator 1 alpha Fructose-induced upregulation PUBTATOR peroxisome proliferator-activated receptor-gamma coactivator 1 alpha 83516 Fructose-induced upregulation of complex II-driven mitochondrial respiration, peroxisome proliferator-activated receptor-gamma coactivator 1 alpha, and peroxisome proliferator activated receptor α also persisted after switching to control diet. 32157140 4 50 gly N-glycosylated 558:571 arg1 Gpr176 Gpr176 PUBTATOR Gpr176 Q14439 Here we show that Gpr176 is N-glycosylated. 32331895 11 67 gly N-glycosylation 1997:2011 arg1 bPepT2 bPepT2 Cterm bPepT2 Q16348 In summary, this study demonstrated that N-glycosylation is critical for the function of bPepT2. 34069226 6 58 gly afucosylated 852:863 arg1 afucosylated hIgG1-TA99 afucosylated hIgG1-TA99 Cterm IgG1 C57Bl/6 mice that were injected intraperitoneally with B16F10-gp75 mouse melanoma developed significantly less metastasis outgrowth after treatment with afucosylated hIgG1-TA99 compared to mice treated with wildtype hhIgG1-TA99. 33206527 10 150 gly N-glycosylation 2055:2069 arg2 the single N-glycosylation site the single N-glycosylation site site To investigate the regulatory effect of the N79 glycan on cellular growth, we mutated the single N-glycosylation site in CES1 from Asn to Gln (CES1-N79Q) via site-directed mutagenesis. 33068929 9 0 part_of gut 1409:1411 arg1 the composition gut the composition PUBTATOR Site gut 110006 position The phycocyanin intervention regulated the composition of the lung and gut microbiota, transformed them into normal state, and reduced the level of LPS, which significantly reduced the abundance of inflammation-related bacteria, and increased the abundance of probiotics that produce short-chain fatty acids. 34839024 3 19 gly glycosylation 474:486 arg2 sixteen potential extracellular N-linked glycosylation sites sixteen potential extracellular N-linked glycosylation sites sites CgToll-3 with sixteen potential extracellular N-linked glycosylation sites and shares the closest phylogenic relationship with molluscan TLRs. 32099050 4 4 gly phospholipase 807:819 arg1 the allergen Api m 1 phospholipase A2 the allergen Api m 1 OGER phospholipase A2 P04054 Tandem mass spectrometry identified the cross-reactive allergens as Der f 15 in HDM, two homologues of the Periplaneta americana cockroach allergen Cr-PI/Per a 3 in ACR and two isoforms of the allergen Api m 1 (phospholipase A2: PLA2) in HBV. 34327998 8 49 gly N-glycosylated 1261:1274 arg1 N-glycosylated sites N-glycosylated sites sites Comparisons of GADS for N-glycosylated sites on several proteins, especially the SARS-CoV-2 spike protein, demonstrate the potential reproducibility of GADS and their utility for comparing site-specific distributions. 32598381 2 56 gly glycosylation 301:313 arg2 an N-linked glycosylation site an N-linked glycosylation site site This novel genotype encoded an N-linked glycosylation site at position 245-247 in the NA protein from clade 3c.2a H3N2 viruses. 32598381 2 56 gly glycosylation 301:313 arg2 position 245-247 position 245-247 position 245 This novel genotype encoded an N-linked glycosylation site at position 245-247 in the NA protein from clade 3c.2a H3N2 viruses. 34348917 5 71 gly RUNX1 989:993 arg1 The O-GlcNAc modification RUNX1 The O-GlcNAc modification PUBTATOR RUNX1 50662 The O-GlcNAc modification of RUNX1 was detected by immunoprecipitation. 33802415 2 50 part_of contain 324:330 arg1 FSHβ subunits AND two sites FSHβ subunits two sites Fterm Site subunits sites Both α and FSHβ subunits contain two sites of N-linked oligosaccharides, which are important for its function. 31859482 9 45 gly glycosites 1513:1522 arg2 glycosites glycosites glycosites Together, our method enables a high-throughput enrichment of glycan, glycosites, and IGPs from biological samples. 33454535 11 57 gly glycosylation 1664:1676 arg2 the most recent N-linked glycosylation site predictors the most recent N-linked glycosylation site predictors site Furthermore, compared to N-GlyDE, GlycoEP, SPRINT-Gly, the most recent N-linked glycosylation site predictors, our model yields higher scores on the above 4 metrics, thus further demonstrating the efficiency of our approach. 34711323 1 27 part_of glycoprotein 143:154 arg1 one O-glycosylation sites glycoprotein one O-glycosylation sites Fterm Site glycoprotein sites Recombinant human erythropoietin (EPO) is a complex therapeutic glycoprotein with three N- and one O-glycosylation sites. 34649588 0 85 gly N-glycoprotein 68:81 arg1 enhanced recombinant N-glycoprotein production enhanced recombinant N-glycoprotein production Fterm N-glycoprotein Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria. 34327998 0 61 gly Glycoproteins 75:87 arg1 Site-Specific Glycan Abundance Distributions proteins Site-Specific Glycan Abundance Distributions Fterm proteins Representing and Comparing Site-Specific Glycan Abundance Distributions of Glycoproteins. 32679889 16 84 gly glycoprotein 2127:2138 arg1 glycoprotein abundance glycoprotein abundance Fterm glycoprotein The most marked changes occurred in glycoprotein abundance for both the VviPGIP1 and NtCAD14 lines. 33436086 0 42 gly glycosylation 9:21 arg1 site 158 protein site Fterm protein site N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responses. 33436086 0 42 gly glycosylation 9:21 arg2 site 158 protein site Fterm protein site N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responses. 33436086 0 42 gly glycosylation 9:21 arg2 site 158 site site N-linked glycosylation at site 158 of the HA protein of H5N6 highly pathogenic avian influenza virus is important for viral biological properties and host immune responses. 32516600 0 6 gly glycosylated 16:27 arg1 a glycosylated asparagine residue a glycosylated asparagine residue asparagine residue Generation of a glycosylated asparagine residue through chemoselective acylation of a glycosylhydrazide. 33409271 5 70 gly protein 949:955 arg1 a C-terminal 8x His-tag 2019-nCoV spike protein a C-terminal 8x His-tag PUBTATOR 2019-nCoV spike protein 43740568 The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 70 gly protein 949:955 arg1 a FLAG-tag 2019-nCoV spike protein a FLAG-tag PUBTATOR 2019-nCoV spike protein 43740568 The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33316563 9 2 gly glycopeptides 1715:1727 arg2 the N-linked endogenous glycopeptides the N-linked endogenous glycopeptides glycopeptides Finally, the MOF packed in pipette tip was applied to selectively capture the N-linked endogenous glycopeptides from a healthy saliva sample and 64 unique endogenous glycopeptides were identified. 33316563 9 25 gly glycopeptides 1783:1795 arg2 64 unique endogenous glycopeptides 64 unique endogenous glycopeptides glycopeptides Finally, the MOF packed in pipette tip was applied to selectively capture the N-linked endogenous glycopeptides from a healthy saliva sample and 64 unique endogenous glycopeptides were identified. 32019882 7 73 gly MGL 1004:1006 arg1 truncated O-linked glycans MGL truncated O-linked glycans PUBTATOR MGL 17312 In an immunocompetent orthotopic glioma mouse model overexpressing truncated O-linked glycans (MGL ligands), high-dimensional mass cytometry revealed a wide heterogeneity of infiltrating myeloid cells with increased infiltration of PD-L1+ TAMs as well as distant alterations in the bone marrow (BM). 34835402 6 21 part_of EnfT 912:915 arg1 the EnfT protease domain EnfT the EnfT protease domain Cterm Site EnfT domain Thus, leader sequences cleavage requires two steps: the first via the EnfT protease domain and the second via extracellular proteases. 34835402 6 69 part_of protease 917:924 arg1 the EnfT protease domain protease the EnfT protease domain Fterm Site protease domain Thus, leader sequences cleavage requires two steps: the first via the EnfT protease domain and the second via extracellular proteases. 32441096 3 41 gly glycoproteins 705:717 arg1 glycoproteins glycoproteins Fterm glycoproteins A new MALDI mass spectrometry imaging workflow in formalin-fixed clinical tissues is described using recombinant endoglycosidase F3 (Endo F3), an enzyme with a specific preference for cleaving core-fucosylated N-glycans attached to glycoproteins. 32441096 3 55 gly attached 693:700 arg2 glycoproteins AND core-fucosylated N-glycans glycoproteins core-fucosylated N-glycans Fterm glycoproteins A new MALDI mass spectrometry imaging workflow in formalin-fixed clinical tissues is described using recombinant endoglycosidase F3 (Endo F3), an enzyme with a specific preference for cleaving core-fucosylated N-glycans attached to glycoproteins. 32441096 3 59 gly core-fucosylated 666:681 arg1 core-fucosylated N-glycans core-fucosylated N-glycans A new MALDI mass spectrometry imaging workflow in formalin-fixed clinical tissues is described using recombinant endoglycosidase F3 (Endo F3), an enzyme with a specific preference for cleaving core-fucosylated N-glycans attached to glycoproteins. 33863420 0 33 gly glycopeptides 129:141 arg2 glycopeptides glycopeptides glycopeptides Dual-functionalized magnetic bimetallic metal-organic framework composite for highly specific enrichments of phosphopeptides and glycopeptides. 33906120 7 77 gly glycoproteins 1071:1083 arg1 These glycoproteins These glycoproteins Fterm glycoproteins These glycoproteins are involved in cell wall reconstruction, biomacromolecular metabolism, signal transduction, endoplasmic reticulum quality control and stress response. 33647064 9 22 part_of sites 1206:1210 arg1 wild-type MACV GPC MACV GPC sites OGER Site MACV GPC P04921 sites On the other hand, disruption of these glycosylation sites on wild-type MACV GPC rendered the virus substantially attenuated in vivo and also more susceptible to antibody neutralization, while loss of these glycans did not affect virus growth in cultured cells. 34677013 1 116 gly proteins 224:231 arg1 O-linked β-N-acetylglucosamine proteins O-linked β-N-acetylglucosamine Fterm proteins O-linked β-N-acetylglucosamine (O-GlcNAc), an important post-translational modification of proteins, plays an essential role in maintaining normal biological activities of organisms. 33550377 5 8 gly O-GlcNAcylation 563:577 arg1 GCs GCs O-GlcNAcylation OGER GCs Q16739 Here, O-GlcNAcylation in GCs and its effects on GC proliferation were determined. 32221039 6 78 gly N-glycosylation 1389:1403 arg2 ex novo N-glycosylation sites ex novo N-glycosylation sites sites In 7/80 RA-rmAb, SHM resulted in ex novo N-glycosylation sites in VH and/or VL regions. 32609386 4 53 gly N-linked-glycosylation 653:674 arg2 potential N-linked-glycosylation sites potential N-linked-glycosylation sites sites HIV gp120 sequences from antiretroviral naïve patients were analyzed for V2 tripeptide composition, length, net charge, and potential N-linked-glycosylation sites. 34773221 0 18 part_of Threonine 36:44 arg1 MeCP2 MeCP2 Threonine PUBTATOR SpecificSite MeCP2 4204 Threonine 203 Loss of O-GlcNAcylation on MeCP2 at Threonine 203 Leads to Neurodevelopmental Disorders. 32518069 4 38 gly glycoproteins 797:809 arg1 membrane glycoproteins membrane glycoproteins Fterm glycoproteins We suggest that negatively charged sialic acids can be modulated, similarly to phosphorylation, by the action of sialyltransferases and sialidases thereby changing local structure and function of membrane glycoproteins. 33409271 3 53 part_of protein 474:480 arg1 The receptor-binding domain protein The receptor-binding domain Fterm Site protein domain The receptor-binding domain (RBD) of the 2019-nCoV spike (S) protein contains disulfide bonds and N-linked glycosylations, therefore, it is typically produced by secretion. 33409271 3 100 part_of receptor-binding 417:432 arg1 The receptor-binding domain receptor The receptor-binding domain Fterm Site receptor domain The receptor-binding domain (RBD) of the 2019-nCoV spike (S) protein contains disulfide bonds and N-linked glycosylations, therefore, it is typically produced by secretion. 33206527 1 66 gly containing 176:185 arg1 a serine esterase AND a unique N-linked glycosyl group a serine esterase a unique N-linked glycosyl group Fterm esterase We previously reported that human carboxylesterase 1 (CES1), a serine esterase containing a unique N-linked glycosyl group at Asn79 (N79 CES1), is a candidate serological marker of hepatocellular carcinoma (HCC). 33206527 1 66 gly containing 176:185 arg1 human carboxylesterase 1 AND a unique N-linked glycosyl group human carboxylesterase 1 a unique N-linked glycosyl group PUBTATOR carboxylesterase 1 1066 We previously reported that human carboxylesterase 1 (CES1), a serine esterase containing a unique N-linked glycosyl group at Asn79 (N79 CES1), is a candidate serological marker of hepatocellular carcinoma (HCC). 31985438 2 41 gly glycosylation 367:379 arg2 glycosylation sites glycosylation sites sites Identification of glycosylation sites in a polypeptide chain is not an easy task due to multiple impediments. 34873473 5 13 gly attached 1003:1010 arg1 MET AND fucosylation status EGFR MET fucosylation status PUBTATOR EGFR 1956 MET Mechanistic investigations revealed that FUT8 was involved in the alteration of fucosylation status that was attached to MET and EGFR, changing MET responses after HGF stimulation, as well as in the transactivation of EGFR. 34373697 3 45 gly isolated 537:544 arg2 A. giraldii Harms AND the homogeneous polysaccharide A. giraldii Harms the homogeneous polysaccharide OGER Harms Q9ULH0 In this study, the structural characterization of the homogeneous polysaccharide isolated from A. giraldii Harms, known as AHP-II, and its immunomodulatory effects in vivo will be studied. 32996013 4 63 gly glycosylation 610:622 arg1 proteins proteins Fterm proteins Hyperglycemia, hyperlipidemia, and hyperinsulinemia lead to an enhancement of protein O-GlcNAcylation; however, whether excessive O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation of proteins in cardiomyocytes causes cardiac hypertrophy remains unclear. 33361116 3 50 gly Afucosylated 319:330 arg1 Afucosylated IgG variants Afucosylated IgG variants Fterm variants Afucosylated IgG variants are already used in anticancer therapeutic antibodies for their increased activity through Fc receptors (FcγRIIIa). 33903632 6 40 part_of GDF15 971:975 arg1 the receptor binding site GDF15 the receptor binding site PUBTATOR Site GDF15 Q99988 site In addition, we identified a set of mutations at the receptor binding site of GDF15 that show increased GFRAL binding affinity and led to significant half-life extension. 33168911 3 59 gly eNOS 573:576 arg1 novel O-GlcNAcylation sites eNOS novel O-GlcNAcylation sites PUBTATOR eNOS 24600 Hence, we aim to determine the effects of glucose deprivation on O-GlcNAcylation and novel O-GlcNAcylation sites of eNOS. 33168911 3 59 gly eNOS 573:576 arg1 O-GlcNAcylation eNOS O-GlcNAcylation PUBTATOR eNOS 24600 Hence, we aim to determine the effects of glucose deprivation on O-GlcNAcylation and novel O-GlcNAcylation sites of eNOS. 32931036 2 28 part_of mGlu7 377:381 arg1 the C-terminal region mGlu7 the C-terminal region Cterm Site mGlu7 2917 region The regulation of mGlu7 trafficking into and out of the plasma membrane by binding proteins within the C-terminal region of mGlu7 governs the bidirectional synaptic plasticity. 34401531 5 60 part_of Gut 704:706 arg1 Gut microbial composition Gut Gut microbial composition PUBTATOR Site Gut 110006 position Gut microbial composition of fecal samples from curdlan-supplemented HFD-fed mice indicated an elevated abundance of Bacteroidetes, whereas a reduced abundance of Firmicutes was noted at the phylum level compared with that in cellulose-supplemented HFD-fed mice. 34155551 3 58 gly glycosylation 620:632 arg1 the SARS-CoV-2 S glycoprotein the SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 In this work, we perform analytical characterization of N- and O-linked glycosylation of the SARS-CoV-2 S glycoprotein. 34155551 3 103 gly glycoprotein 654:665 arg1 the SARS-CoV-2 S glycoprotein the SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 In this work, we perform analytical characterization of N- and O-linked glycosylation of the SARS-CoV-2 S glycoprotein. 34215068 0 63 gly glycopeptides 139:151 arg2 glycopeptides glycopeptides glycopeptides Flexible and hierarchical metal-organic framework composite as solid-phase media for facile affinity-tip fabrication to selectively enrich glycopeptides and phosphopeptides. 34418170 12 19 gly [Ca2+ 1738:1742 arg1 O-GlcNAcylation Ca2 O-GlcNAcylation OGER Ca2 P00918 O-GlcNAcylation and [Ca2+ ]i showed an interaction effect on the expression of osteogenic markers. 34544801 0 86 gly Glycosylation 18:30 arg1 IgG V Regions IgG V Regions Cterm IgG V Regions Elevated N-Linked Glycosylation of IgG V Regions in Myasthenia Gravis Disease Subtypes. 32201074 10 22 gly O-GlcNAc 1206:1213 arg1 EGF domains EGF domains EGF domains Site domains Thus, O-GlcNAc on EGF domains possesses a shared function in the stability of EGF domains and Notch1 trafficking. 34056367 9 108 part_of contained 1582:1590 arg1 a hydrophobic WALP-NVT peptide AND the glycosylation site Asn-Val-Thr site Asn-Val-Thr site Asn-Val-Thr The stepwise method consisted of the glycosylation of a soluble 7-residue peptide, a hydrophobic WALP-NVT peptide, and a γ-sarcoglycan membrane protein, all of which contained the glycosylation site Asn-Val-Thr (NVT). 34030043 5 12 gly N-glycosylation 661:675 arg2 the N-glycosylation sites the N-glycosylation sites sites Taken together, these findings suggest that the N-glycosylation sites on TIGIT, especially residue N101, may be potential targets for PVR/TIGIT immune checkpoint blockade. 34030043 5 12 gly N-glycosylation 661:675 arg2 residue N101 residue N101 N101 Taken together, these findings suggest that the N-glycosylation sites on TIGIT, especially residue N101, may be potential targets for PVR/TIGIT immune checkpoint blockade. 33144327 4 2 gly glycosylated 614:625 arg1 glycosylated β subunit glycosylated β subunit Fterm subunit Na,K-ATPase is made in the endoplasmic reticulum (ER), but the glycan-free catalytic α subunit complexes with glycosylated β subunit in the ER to proceed through Golgi and post-Golgi trafficking. 33359854 8 35 gly protein 1207:1213 arg1 carbohydrates protein carbohydrates Fterm protein The various nutritional parameters were analyzed, in particular the amounts of protein, carbohydrates, ash, fiber, and total lipids. 33689141 2 51 gly glycoprotein 236:247 arg1 The glycoprotein complex The glycoprotein complex Fterm glycoprotein The glycoprotein complex (GPC) contains eleven N-linked glycans that play essential roles in GPC functionalities such as cleavage, transport, receptor recognition, epitope shielding, and immune response. 33689141 2 84 gly contains 263:270 arg1 GPC AND eleven N-linked glycans GPC eleven N-linked glycans OGER GPC Q78HU7 The glycoprotein complex (GPC) contains eleven N-linked glycans that play essential roles in GPC functionalities such as cleavage, transport, receptor recognition, epitope shielding, and immune response. 33863420 4 31 part_of IgG 1040:1042 arg1 29 phosphopeptides IgG 29 phosphopeptides Cterm Site IgG phosphopeptides By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 33863420 4 31 part_of IgG 1040:1042 arg1 24 glycopeptides IgG 24 glycopeptides Cterm Site IgG glycopeptides By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 33863420 4 37 part_of α-casein 1027:1034 arg1 29 phosphopeptides α-casein 29 phosphopeptides Fterm Site α-casein phosphopeptides By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 33863420 4 37 part_of α-casein 1027:1034 arg1 24 glycopeptides α-casein 24 glycopeptides Fterm Site α-casein glycopeptides By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 32117864 1 96 gly glycoproteins 246:258 arg1 glycoproteins glycoproteins Fterm glycoproteins Sialylation, a post-translational modification that impacts the structure, activity, and longevity of glycoproteins has been thought to be controlled primarily by the expression of sialyltransferases (STs). 31724933 2 11 gly glycoproteins 257:269 arg1 the viral glycoproteins the viral glycoproteins Fterm glycoproteins Virions travel through the foregut and enter midgut epithelial cells through the interaction between the viral glycoproteins and cellular receptors. 34668190 0 19 gly p65 62:64 arg1 O-GlcNAcylation p65 O-GlcNAcylation PUBTATOR p65 19697 OGT upregulates myogenic IL-6 by mediating O-GlcNAcylation of p65 in mouse skeletal muscle under cold exposure. 34725712 8 21 part_of TPR 1541:1543 arg1 the TPR domain TPR the TPR domain OGER Site TPR P12270 domain This dataset provides a foundation to analyze how perturbations to the TPR domain and expression of OGT isoforms affect the glycosylation of substrates, which will be critical for future efforts in protein engineering of OGT, the biology of OGT isoforms, and diseases associated with the TPR domain of OGT. 34725712 8 57 part_of TPR 1324:1326 arg1 TPR domain TPR TPR domain OGER Site TPR P12270 domain This dataset provides a foundation to analyze how perturbations to the TPR domain and expression of OGT isoforms affect the glycosylation of substrates, which will be critical for future efforts in protein engineering of OGT, the biology of OGT isoforms, and diseases associated with the TPR domain of OGT. 34725712 8 63 part_of OGT 1555:1557 arg1 the TPR domain OGT the TPR domain PUBTATOR Site OGT 8473 domain This dataset provides a foundation to analyze how perturbations to the TPR domain and expression of OGT isoforms affect the glycosylation of substrates, which will be critical for future efforts in protein engineering of OGT, the biology of OGT isoforms, and diseases associated with the TPR domain of OGT. 34725712 8 71 part_of isoforms 1357:1364 arg1 TPR domain OGT isoforms TPR domain PUBTATOR Site OGT isoforms 8473 domain This dataset provides a foundation to analyze how perturbations to the TPR domain and expression of OGT isoforms affect the glycosylation of substrates, which will be critical for future efforts in protein engineering of OGT, the biology of OGT isoforms, and diseases associated with the TPR domain of OGT. 33886622 8 69 gly N-glycosylated 1420:1433 arg1 PmCOX PmCOX OGER COX Similarly, addition of tunicamycin, which inhibits N-linked glycosylation, in PmCOX-expressing cells resulted in PmCOX protein with lower molecular mass than those obtained from untreated cells, suggesting that PmCOX was N-glycosylated. 32518939 1 28 gly positions 294:302 arg1 two positions two positions positions Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 32518939 1 33 gly found 281:285 arg1 two positions AND O-glucose two positions O-glucose positions Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 32518939 1 33 gly found 281:285 arg2 two positions AND O-Glc two positions O-Glc positions Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 32518939 1 72 gly modified 179:186 arg1 Epidermal growth factor-like domains AND N-glycans EGF domains N-glycans OGER EGF P01132 domains Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 32518939 1 72 gly modified 179:186 arg1 secreted proteins AND N-glycans proteins domains N-glycans Fterm proteins domains Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 33886622 12 63 gly N-glycosylation 1884:1898 arg2 N-glycosylation sites N-glycosylation sites sites This study not only establishes the presence of the COX enzyme in penaeid shrimp, but also reveals that N-glycosylation sites are highly conserved and required for COX function in crustaceans. 34644163 2 46 part_of protein 424:430 arg1 the C-terminal domain protein the C-terminal domain Fterm Site protein domain It digests crystalline cellulose with an unknown mechanism and possesses a type IX secretion system (T9SS) that can recognize the C-terminal domain (CTD) of the cargo protein as a signal. 33889548 10 70 gly glycopeptides 1605:1617 arg2 The two glycopeptides The two glycopeptides glycopeptides The two glycopeptides HAN253WTLTPLK (H5N4S2) and (H5N4S1) corresponding to PON1 were significantly increased in AFP-negative HCC patients, as compared with LC patients. 32892942 10 102 gly N-glycopeptides 1799:1813 arg2 the low-abundance N-glycopeptides the low-abundance N-glycopeptides N-glycopeptides All the experiments demonstrated that this polyacrylamide-based hydrophilic microsphere shows a great potential to enrich the low-abundance N-glycopeptides for glycoproteome analysis of real complicated biological samples. 32700722 1 9 gly glycosylated 100:111 arg1 a glycosylated class III lanthipeptide a glycosylated class III lanthipeptide lanthipeptide NAI-112 is a glycosylated class III lanthipeptide produced by an Actinoplanes sp. 34693225 0 53 gly glycopeptide 39:50 arg2 A pain-causing and paralytic ant venom glycopeptide A pain-causing and paralytic ant venom glycopeptide glycopeptide A pain-causing and paralytic ant venom glycopeptide. 32511336 3 14 gly S 355:355 arg1 22 N-linked glycan sequons S 22 N-linked glycan sequons PUBTATOR S 43740568 SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in immune evasion and occluding immunogenic protein epitopes. 32511336 3 21 gly SARS-CoV-2 344:353 arg1 22 N-linked glycan sequons SARS 22 N-linked glycan sequons OGER SARS P49591 SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in immune evasion and occluding immunogenic protein epitopes. 34773221 3 30 gly modified 574:581 arg1 MeCP2 AND O-linked-β-N-acetylglucosamine MeCP2 O-linked-β-N-acetylglucosamine PUBTATOR MeCP2 4204 In this study, we demonstrated that MeCP2 was dynamically modified by O-linked-β-N-acetylglucosamine (O-GlcNAc) at threonine 203 (T203), an etiologic site in RTT patients. 34773221 3 30 gly modified 574:581 arg1 MeCP2 AND O-GlcNAc MeCP2 O-GlcNAc PUBTATOR MeCP2 4204 In this study, we demonstrated that MeCP2 was dynamically modified by O-linked-β-N-acetylglucosamine (O-GlcNAc) at threonine 203 (T203), an etiologic site in RTT patients. 32518069 3 15 gly glycopeptides 577:589 arg2 formerly sialylated N-linked glycopeptides formerly sialylated N-linked glycopeptides glycopeptides Here we show that sialylation of N-linked glycosylation is a novel potential modulator of neurotransmitter release mechanisms by investigating depolarization-dependent changes of formerly sialylated N-linked glycopeptides. 33665237 1 96 gly glycoproteins 235:247 arg1 glycoproteins glycoproteins Fterm glycoproteins 25333 Bone biomineralization is a complex process in which type I collagen and associated non-collagenous proteins (NCPs), including glycoproteins and proteoglycans, interact closely with inorganic calcium and phosphate ions to control the precipitation of nanosized, non-stoichiometric hydroxyapatite (HAP, idealized stoichiometry Ca10(PO4)6(OH)2) within the organic matrix of a tissue. 32046000 10 62 gly KLH-glycopeptide 1690:1705 arg2 KLH-glycopeptide KLH-glycopeptide KLH-glycopeptide To determine whether antibodies to these peptides could modify persistence of spores within the gut, animals immunized intranasally with either the KLH-glycopeptide or KLH-peptide conjugate in the presence of cholera toxin, were challenged with R20291 spores. 32598381 2 29 part_of site 315:318 arg1 the NA protein protein site Fterm Site protein site This novel genotype encoded an N-linked glycosylation site at position 245-247 in the NA protein from clade 3c.2a H3N2 viruses. 34459483 9 3 gly sialylated 1583:1592 arg1 PRG4 PRG4 OGER PRG4 Q92954 The combined results demonstrate that, in addition to platelet GPIbα, PRG4 is a highly sialylated mucin-like glycoprotein found in aortic valve vegetations and may contribute to the persistence of oral streptococci in this protected endovascular niche. 34459483 9 3 gly sialylated 1583:1592 arg1 a highly sialylated mucin-like glycoprotein a highly sialylated mucin-like glycoprotein Fterm glycoprotein The combined results demonstrate that, in addition to platelet GPIbα, PRG4 is a highly sialylated mucin-like glycoprotein found in aortic valve vegetations and may contribute to the persistence of oral streptococci in this protected endovascular niche. 34459483 9 7 gly glycoprotein 1605:1616 arg1 PRG4 PRG4 OGER PRG4 Q92954 The combined results demonstrate that, in addition to platelet GPIbα, PRG4 is a highly sialylated mucin-like glycoprotein found in aortic valve vegetations and may contribute to the persistence of oral streptococci in this protected endovascular niche. 34459483 9 7 gly glycoprotein 1605:1616 arg1 a highly sialylated mucin-like glycoprotein a highly sialylated mucin-like glycoprotein Fterm glycoprotein The combined results demonstrate that, in addition to platelet GPIbα, PRG4 is a highly sialylated mucin-like glycoprotein found in aortic valve vegetations and may contribute to the persistence of oral streptococci in this protected endovascular niche. 32482891 11 23 gly glycosylation 1907:1919 arg1 ACLP ACLP PUBTATOR ACLP 165 Our findings highlight the importance of N-linked glycosylation of ACLP for its secretion and contribute to our understanding of ACLP-dependent disease pathologies. 32892442 2 73 gly proteins 413:420 arg1 O-GlcNAc moiety proteins O-GlcNAc moiety Fterm proteins O-linked-N-acetylglucosamine (O-GlcNAc) transferase (OGT) is an essential cellular enzyme that posttranslationally modifies the cellular proteins with O-GlcNAc moiety. 33552834 4 24 part_of receptor 994:1001 arg1 receptor binding domain receptor receptor binding domain Fterm Site receptor domain Our comprehensive structure analysis revealed that the natural substitution of amino acid residues Gln24, His34, Phe40, Leu79 and Met82 in the N-terminal α1 and α2 helices of the ACE2 receptor results in loss of crucial network of hydrogen-bonded and hydrophobic interactions with receptor binding domain of SARS-CoV-2 spike protein. 33552834 4 104 part_of protein 1038:1044 arg1 receptor binding domain spike protein receptor binding domain PUBTATOR Site spike protein 43740568 domain Our comprehensive structure analysis revealed that the natural substitution of amino acid residues Gln24, His34, Phe40, Leu79 and Met82 in the N-terminal α1 and α2 helices of the ACE2 receptor results in loss of crucial network of hydrogen-bonded and hydrophobic interactions with receptor binding domain of SARS-CoV-2 spike protein. 32851535 3 34 gly glycopeptides 667:679 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides By taking advantages of the porous structure, abundant binding sites, and multivalent interactions between glycopeptides and both glutathione molecules and free amino groups, the pGP/AuG is adopted to the selective enrichment of N-linked glycopeptides with low limit of detection (2 fmol), high enrichment selectivity (1:500), binding capacity (333.3 mg/g), recovery yield (91.3 ± 2.1%), and repeatability (< 6.0% RSD) using matrix-assisted laser desorption/ionization time of flight mass spectrometry detection method. 32851535 3 44 gly glycopeptides 536:548 arg2 glycopeptides glycopeptides glycopeptides By taking advantages of the porous structure, abundant binding sites, and multivalent interactions between glycopeptides and both glutathione molecules and free amino groups, the pGP/AuG is adopted to the selective enrichment of N-linked glycopeptides with low limit of detection (2 fmol), high enrichment selectivity (1:500), binding capacity (333.3 mg/g), recovery yield (91.3 ± 2.1%), and repeatability (< 6.0% RSD) using matrix-assisted laser desorption/ionization time of flight mass spectrometry detection method. 34030043 5 28 part_of sites 677:681 arg1 TIGIT TIGIT sites PUBTATOR Site TIGIT 201633 sites Taken together, these findings suggest that the N-glycosylation sites on TIGIT, especially residue N101, may be potential targets for PVR/TIGIT immune checkpoint blockade. 32331895 1 72 gly N-glycosylation 282:296 arg2 multiple putative N-glycosylation sites multiple putative N-glycosylation sites sites Bovine peptide transporter 2 (bPepT2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative N-glycosylation sites of asparagine residues. 32331895 1 72 gly N-glycosylation 282:296 arg2 asparagine residues asparagine residues asparagine residues Bovine peptide transporter 2 (bPepT2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative N-glycosylation sites of asparagine residues. 32910214 9 10 part_of multi-glycosylated 1260:1277 arg1 mono- and multi-glycosylated peptides multi mono- and multi-glycosylated peptides OGER Site multi peptides Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample. 34322281 10 9 part_of enzyme 1429:1434 arg1 enzyme residues R362, K363, Q364, H365 and W331, which modulate the pocket size and specific enzyme-peptide interactions enzyme enzyme residues R362, K363, Q364, H365 and W331, which modulate the pocket size and specific enzyme-peptide interactions Fterm Site enzyme residues The preference for specific residues at the -1 position of the peptide was regulated by enzyme residues R362, K363, Q364, H365 and W331, which modulate the pocket size and specific enzyme-peptide interactions. 34056367 12 19 part_of contain 2124:2130 arg1 small or large membrane proteins AND an N-glycosylation consensus sequence small or large membrane proteins an N-glycosylation consensus sequence Fterm Site proteins sequence Our experiments demonstrated that small or large membrane proteins that contain an N-glycosylation consensus sequence can be glycosylated by NGT in membrane-mimetic environments. 34408013 4 32 part_of C1q 637:639 arg1 the globular heads C1q the globular heads PUBTATOR Site C1q 712 heads This hexamerization seems crucial for IgG to enable efficient interaction with the globular heads of the first complement component C1q and subsequent complement activation. 34524799 6 24 gly glycoproteins 1039:1051 arg1 glycoproteins glycoproteins Fterm glycoproteins Such staining selectivity has never been observed in conventional dyes, suggesting that PRM-EA could serve as a unique staining agent for the selective detection of glycoproteins with terminal Man residues. 34227365 11 198 gly N-glycoprotein/N-glycopeptide 2164:2192 arg2 N-glycoprotein/N-glycopeptide N-glycoprotein/N-glycopeptide N-glycoprotein/N-glycopeptide The consistency of N-glycoprotein/N-glycopeptide levels in urine samples taken from the same healthy person for five consecutive days was investigated by correlation analysis. 33924774 3 21 gly glycosylation 451:463 arg2 N-linked glycosylation sites N-linked glycosylation sites sites Similar to other mammalian receptors, ANTXR1 has been shown to harbor N-linked glycosylation sites in its extracellular vWA domain. 33886622 5 64 part_of COX 822:824 arg1 putative catalytic domains COX putative catalytic domains OGER Site COX domains Sequence analysis revealed that essential catalytic residues and putative catalytic domains of P. monodon COX (PmCOX) were well-conserved in relation to other vertebrate and crustacean COXs. 33886622 5 64 part_of COX 822:824 arg1 essential catalytic residues COX essential catalytic residues OGER Site COX residues Sequence analysis revealed that essential catalytic residues and putative catalytic domains of P. monodon COX (PmCOX) were well-conserved in relation to other vertebrate and crustacean COXs. 34408013 1 3 gly contains 121:128 arg1 position 297 AND one evolutionarily conserved N-linked glycan Human IgG position 297 one evolutionarily conserved N-linked glycan Cterm Human Ig position 297 Human IgG contains one evolutionarily conserved N-linked glycan in its Fc region at position 297. 34408013 1 3 gly contains 121:128 arg1 Human IgG AND one evolutionarily conserved N-linked glycan Human Ig region one evolutionarily conserved N-linked glycan Cterm Human Ig region Human IgG contains one evolutionarily conserved N-linked glycan in its Fc region at position 297. 32084687 5 10 gly origin 970:975 arg1 complex polysaccharides origin complex polysaccharides Fterm origin For instance, the microbiota of the digestive gland is characterized by Ruminococcaceae and Lachnospiraceae, being shaped to ferment complex polysaccharides of dietary origin into short-chain fatty acids, well matching the general asset of the animal gut microbiota. 33733058 4 45 gly composition 891:901 arg1 virus entry receptors receptors composition Fterm receptors Glycan composition on virus entry receptors has been shown to influence the rate of infection of SARS-CoV-2 and spreading of virions has recently been linked to altered lysosomal exocytosis. 34562451 1 22 gly glycosylation 243:255 arg2 four N-linked glycosylation sites four N-linked glycosylation sites sites TMPRSS13, a member of the type II transmembrane serine protease (TTSP) family, harbors four N-linked glycosylation sites in its extracellular domain. 31985438 1 58 gly Glycosylation 136:148 arg1 proteins proteins Fterm proteins Glycosylation of proteins in eukaryote cells is an important and complicated post-translation modification due to its pivotal role and association with crucial physiological functions within most of the proteins. 34925382 2 13 gly modified 530:537 arg3 HLA Class I peptides AND GlcNAc HLA Class I peptides GlcNAc peptides Here we concentrate on the latter, focusing on the small subset of HLA Class I peptides (less than 1%) that has been observed to be post-translationally modified (PTM) by a O-linked N-acetylglucosamine (GlcNAc). 34925382 2 13 gly modified 530:537 arg3 HLA Class I peptides AND a O-linked N-acetylglucosamine HLA Class I peptides a O-linked N-acetylglucosamine peptides Here we concentrate on the latter, focusing on the small subset of HLA Class I peptides (less than 1%) that has been observed to be post-translationally modified (PTM) by a O-linked N-acetylglucosamine (GlcNAc). 33370382 4 10 gly glycosylation 626:638 arg1 virus-derived Env virus-derived Env PUBTATOR Env 100616444 Here, we aimed to understand the impact of the SP diversity on glycosylation of virus-derived Env and virus neutralization by swapping SPs. 31883117 3 20 part_of lignin 496:501 arg1 composition lignin composition Fterm Site lignin position Various analytical techniques and microscopy methods were employed to examine the composition and distribution of lignin and structural polysaccharides in leatherwood xylem in comparison with trembling aspen (Populus tremuloides) and white spruce (Picea glauca). 33886622 10 19 gly glycosylated 1587:1598 arg1 all three residues all three residues residues Mutational analysis revealed that although all three residues were glycosylated, only mutations at N170 and N424 completely abolished catalytic function. 32766576 2 64 part_of receptor 272:279 arg1 protein's receptor binding domain receptor protein's receptor binding domain Fterm Site receptor domain SARS-CoV-2 (the virus causing COVID-19) is known to use its surface spike (S) protein's receptor binding domain (RBD) to interact with the angiotensin-converting enzyme 2 (ACE2) receptor expressed on many human cell types. 33603735 5 21 part_of MAVS 998:1001 arg1 the serine-rich region MAVS the serine-rich region PUBTATOR Site MAVS 57506 region O-GlcNAcylation of the serine-rich region of MAVS also suppresses its interaction with TRAF3; this prevents IRF3 activation and production of interferon-β. 34555499 1 1 gly glycoproteins 157:169 arg1 ovarian cancer (OC) glycoproteins ovarian cancer (OC) glycoproteins Fterm glycoproteins 3958 Despite sulfated O-linked glycans being abundant on ovarian cancer (OC) glycoproteins, their regulation during cancer development and involvement in cancer pathogenesis remain unexplored. 34418170 6 21 gly [Ca2+ 1070:1074 arg1 O-GlcNAcylation Ca2 O-GlcNAcylation OGER Ca2 P00918 The interaction effect between O-GlcNAcylation and [Ca2+ ]i on osteogenic marker expression was determined using stable OGT knockdown MC3T3-E1 cells. 33168911 15 88 gly eNOS 2205:2208 arg1 O-GlcNAcylation eNOS O-GlcNAcylation PUBTATOR eNOS 4846 Glucose deprivation increases O-GlcNAcylation and activity of eNOS, potentially by the AMPK-OGT pathway, suggesting that Thr866 is a novel O-GlcNAcylation site involved in glucose-deprivation mediated eNOS activation. 34452648 3 62 gly O-glycosylated 377:390 arg1 proteins proteins Fterm proteins However, proteins that can be O-glycosylated by C1GALT1 in GC have not been completely elucidated. 34062155 8 49 gly glycosylation 1354:1366 arg1 torsinA torsinA PUBTATOR torsinA 1861 Using a yeast torsinA expression system, we demonstrate that a specific protein disulfide isomerase, Pdi1, affects the folding and N-linked glycosylation of torsinA and torsinA∆E in a redox-dependent manner, suggesting that the acquisition of early torsinA folding intermediates is sensitive to perturbed interactions between Cys residues and the quality control machinery. 34227365 13 61 gly N-glycoproteins/N-glycopeptides 2560:2590 arg2 N-glycoproteins/N-glycopeptides N-glycoproteins/N-glycopeptides N-glycoproteins/N-glycopeptides Next, urinary samples from 20 healthy male volunteers and 20 healthy female volunteers were enriched for N-glycoproteins/N-glycopeptides, which were profiled by MS through qualitative and quantitative analyses. 31996435 4 77 gly glycoprotein 747:758 arg1 the glycoprotein complex the glycoprotein complex Fterm glycoprotein The glycoprotein (GPC) gene is primarily responsible for attenuation of the Can strain, and we have shown that the absence of an N-linked glycosylation motif in the subunit G1 of the glycoprotein complex of Can, which is otherwise present in the wild-type pathogenic JUNV, causes GPC retention in the endoplasmic reticulum (ER). 31996435 4 99 gly glycoprotein 568:579 arg1 The glycoprotein (GPC) gene The glycoprotein (GPC) gene Fterm glycoprotein The glycoprotein (GPC) gene is primarily responsible for attenuation of the Can strain, and we have shown that the absence of an N-linked glycosylation motif in the subunit G1 of the glycoprotein complex of Can, which is otherwise present in the wild-type pathogenic JUNV, causes GPC retention in the endoplasmic reticulum (ER). 31996435 4 106 gly glycosylation 702:714 arg2 an N-linked glycosylation motif an N-linked glycosylation motif motif The glycoprotein (GPC) gene is primarily responsible for attenuation of the Can strain, and we have shown that the absence of an N-linked glycosylation motif in the subunit G1 of the glycoprotein complex of Can, which is otherwise present in the wild-type pathogenic JUNV, causes GPC retention in the endoplasmic reticulum (ER). 32878894 10 41 gly sites 2424:2428 arg1 the glycosylation sites the glycosylation sites sites In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32878894 10 41 gly sites 2424:2428 arg1 N11 N11 N11 In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32878894 10 134 gly cysteines 2447:2455 arg1 cysteines cysteines cysteines In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32878894 10 18 gly glycosylation 2410:2422 arg1 gp85 gp85 sites Cterm gp85 sites In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32878894 10 18 gly glycosylation 2410:2422 arg2 N11 gp85 N11 Cterm gp85 N11 In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 33415696 4 25 gly glycoproteins 793:805 arg1 small integrin-binding ligand N-linked glycoproteins small integrin-binding ligand N-linked glycoproteins Fterm glycoproteins The probability of loss-of-function intolerance (pLI) differed widely within members of the thrombospondin, CYR61/CTGF/NOV (CCN), tenascin, small integrin-binding ligand N-linked glycoproteins (SIBLING), and secreted protein, acidic and rich in cysteine (SPARC) gene families. 34260942 4 51 part_of Psme3 731:735 arg1 serine 111 Psme3 serine 111 PUBTATOR SpecificSite Psme3 19192 serine 111 Mechanistically, O-GlcNAc modification of serine 111 (S111) of Psme3 promotes degradation of Ddx6, which is essential for processing body (P-body) assembly, resulting in the maintenance of ESC pluripotent state. 33682335 12 68 gly glycoprotein 1470:1481 arg1 glycoprotein analysis glycoprotein analysis Fterm glycoprotein The findings are possibly broadly relevant to glycoprotein analysis since the structural motif involved in internal hydrogen bonding is common to N-linked glycans of human glycoproteins. 33682335 12 52 gly glycoproteins 1596:1608 arg1 N-linked glycans glycoproteins N-linked glycans Fterm glycoproteins The findings are possibly broadly relevant to glycoprotein analysis since the structural motif involved in internal hydrogen bonding is common to N-linked glycans of human glycoproteins. 33140951 10 2 part_of containing 1699:1708 arg1 peptides AND ADP-ribosylated arginine peptides ADP-ribosylated arginine arginine Taking peptide fragment ions resulting from this specific cleavage into account, a considerably larger number of peptides containing ADP-ribosylated arginine were identified in database searches. 34725712 0 31 part_of TPR 18:20 arg1 the TPR domain TPR the TPR domain OGER Site TPR P12270 domain Truncation of the TPR domain of OGT alters substrate and glycosite selection. 34725712 0 59 part_of OGT 32:34 arg1 the TPR domain OGT the TPR domain PUBTATOR Site OGT 8473 domain Truncation of the TPR domain of OGT alters substrate and glycosite selection. 34490144 6 24 gly hyperglycosylated 927:943 arg1 YghJ YghJ PUBTATOR YghJ 2847716 Using the mass spectrometry-based method BEMAP, we demonstrate that YghJ is hyperglycosylated in ETEC and identify 54 O-linked Set/Thr residues within the 1519 amino acid primary sequence. 34030043 0 30 gly glycosylations 13:26 arg1 Asn101 Asn32 and Asn101 Asn32 and Asn101 The N-linked glycosylations of TIGIT Asn32 and Asn101 facilitate PVR/TIGIT interaction. 34342946 6 53 gly glycoprotein 1188:1199 arg1 the human myelin oligodendrocyte glycoprotein the human myelin oligodendrocyte glycoprotein PUBTATOR myelin oligodendrocyte glycoprotein 4340 While there is less structural resemblance to members of the human butyrophilin family such as BTN3A, which play a role as immune receptors, the N-terminal bovine butyrophilin subdomain shows surprising similarity to the human myelin oligodendrocyte glycoprotein, a protein exposed on the surface of myelin sheaths. 34342946 6 53 gly glycoprotein 1188:1199 arg1 a protein a protein Fterm protein While there is less structural resemblance to members of the human butyrophilin family such as BTN3A, which play a role as immune receptors, the N-terminal bovine butyrophilin subdomain shows surprising similarity to the human myelin oligodendrocyte glycoprotein, a protein exposed on the surface of myelin sheaths. 33454535 3 34 part_of sites 465:469 arg1 ion channel proteins proteins sites Fterm Site proteins sites This study used a computational method to predict N-linked glycosylation sites, the most common type, in ion channel proteins. 34227365 19 8 gly N-glycoproteins 3266:3280 arg1 the N-glycoproteins the N-glycoproteins Fterm N-glycoproteins Functional analysis of the N-glycoproteins that exhibited gender differences in abundance was performed. 34622651 8 44 gly glycopeptides 1231:1243 arg2 Intact glycopeptides Intact glycopeptides glycopeptides Intact glycopeptides isolated from human serum were used to evaluate the performance of the integrated platform. 34622651 8 63 gly used 1276:1279 arg2 Intact glycopeptides Intact glycopeptides glycopeptides Intact glycopeptides isolated from human serum were used to evaluate the performance of the integrated platform. 33278450 2 25 part_of receptor 411:418 arg1 receptor site receptor receptor site Fterm Site receptor site In silico study was done to explore interactions of ropinirole and thymoquinone at receptor site (TNF-α and NFK-β). 33118239 3 5 gly glycoprotein 453:464 arg1 glycoprotein synthesis glycoprotein synthesis Fterm glycoprotein In order to investigate the applicability of this reaction to glycoprotein synthesis, we synthesized evasin-3, a cysteine-rich glycoprotein with chemokine-binding ability originally found in tick saliva. 33118239 3 38 gly glycoprotein 518:529 arg1 a cysteine-rich glycoprotein a cysteine-rich glycoprotein Fterm glycoprotein In order to investigate the applicability of this reaction to glycoprotein synthesis, we synthesized evasin-3, a cysteine-rich glycoprotein with chemokine-binding ability originally found in tick saliva. 33034768 0 18 part_of Composition 57:67 arg1 Siganus oramin oramin Composition Fterm Site oramin position Enteromorpha prolifera Diet Drives Intestinal Microbiome Composition in Siganus oramin. 34483834 4 9 gly glycosylation 520:532 arg2 2 N-linked glycosylation sites 2 N-linked glycosylation sites sites Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. 34483834 4 12 gly glycosylation 598:610 arg2 position 27 PKR2 position 27 PUBTATOR PKR2 246313 position 27 Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. 34483834 4 12 gly glycosylation 598:610 arg1 position 27 PKR2 position 27 PUBTATOR PKR2 246313 position 27 Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. 34483834 4 12 gly glycosylation 598:610 arg1 position 27 position 27 position 27 Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. 34215103 5 33 gly N-glycosylation 1215:1229 arg2 125 N-glycosylation sites 125 N-glycosylation sites sites 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 34215103 5 90 gly glycopeptides 1431:1443 arg2 endogenous N-linked glycopeptides endogenous N-linked glycopeptides glycopeptides 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 34215103 5 110 gly glycoproteins 1266:1278 arg1 65 glycoproteins 65 glycoproteins Fterm glycoproteins 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 34215103 5 33 gly N-glycosylation 1215:1229 arg2 134 glycopeptides glycopeptides glycopeptides 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 34215103 5 52 gly glycopeptides 1244:1256 arg1 65 glycoproteins glycoproteins glycopeptides Fterm glycoproteins glycopeptides 125 N-glycosylation sites of 134 glycopeptides from 65 glycoproteins were identified from 2 μL sample of human serum treated with the MPDA@PEI@CA nanoparticles, which manifested the ability to enrich endogenous N-linked glycopeptides from complex biological samples. 33914740 2 58 gly detected 428:435 arg1 the P.pastoris-derived rBer e 1 AND O-linked glycans the P.pastoris-derived rBer e 1 O-linked glycans PUBTATOR e 1 However, O-linked glycans was detected on the P.pastoris-derived rBer e 1, which is not naturally present in nBer e 1, and may contribute to the allergic sensitisation. 35091346 8 10 gly cofactors 1648:1656 arg1 1951 O-GlcNAc peptides cofactors 1951 O-GlcNAc peptides Fterm cofactors Deep coverage of OCAPs was achieved from HeLa cells, including 1951 O-GlcNAc peptides from 1136 O-GlcNAc chromatin-associated transcription factors and cofactors (TFCs) using HCD fragmentation and 669 O-GlcNAc sites using EThcD fragmentation. 35091346 8 40 gly HeLa 1537:1540 arg1 1951 O-GlcNAc peptides HeLa 1951 O-GlcNAc peptides OGER HeLa P0DMC3 Deep coverage of OCAPs was achieved from HeLa cells, including 1951 O-GlcNAc peptides from 1136 O-GlcNAc chromatin-associated transcription factors and cofactors (TFCs) using HCD fragmentation and 669 O-GlcNAc sites using EThcD fragmentation. 35091346 8 42 gly factors 1636:1642 arg1 1951 O-GlcNAc peptides factors 1951 O-GlcNAc peptides Fterm factors Deep coverage of OCAPs was achieved from HeLa cells, including 1951 O-GlcNAc peptides from 1136 O-GlcNAc chromatin-associated transcription factors and cofactors (TFCs) using HCD fragmentation and 669 O-GlcNAc sites using EThcD fragmentation. 34866967 0 10 gly pumpkin 68:74 arg1 polysaccharides pumpkin polysaccharides Fterm pumpkin Optimization of aqueous-assisted extraction of polysaccharides from pumpkin (Cucurbita moschata Duch) and their biological activities. 32295844 7 13 gly Thr-577 1277:1283 arg1 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 We demonstrate here that MYPT1 is O-GlcNAcylated at Thr-577, Ser-585, Ser-589, and Ser-601, which antagonizes CDK1-dependent phosphorylation at Ser-473 and attenuates the association between MYPT1 and PLK1, thereby promoting PLK1 activity. 32295844 7 49 gly Ser-585 1286:1292 arg1 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 We demonstrate here that MYPT1 is O-GlcNAcylated at Thr-577, Ser-585, Ser-589, and Ser-601, which antagonizes CDK1-dependent phosphorylation at Ser-473 and attenuates the association between MYPT1 and PLK1, thereby promoting PLK1 activity. 32295844 7 63 gly Ser-589 1295:1301 arg1 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 We demonstrate here that MYPT1 is O-GlcNAcylated at Thr-577, Ser-585, Ser-589, and Ser-601, which antagonizes CDK1-dependent phosphorylation at Ser-473 and attenuates the association between MYPT1 and PLK1, thereby promoting PLK1 activity. 32295844 7 73 gly Ser-601 1308:1314 arg1 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 O-GlcNAcylated Thr-577, Ser-585, Ser-589, and Ser-601 We demonstrate here that MYPT1 is O-GlcNAcylated at Thr-577, Ser-585, Ser-589, and Ser-601, which antagonizes CDK1-dependent phosphorylation at Ser-473 and attenuates the association between MYPT1 and PLK1, thereby promoting PLK1 activity. 32157140 0 74 gly glycosylation 59:71 arg1 the orphan G protein-coupled receptor Gpr176 the orphan G protein-coupled receptor Gpr176 OGER Gpr176 Q14439 Identification and functional characterisation of N-linked glycosylation of the orphan G protein-coupled receptor Gpr176. 32851535 5 2 gly glycopeptides 1464:1476 arg2 glycopeptides glycopeptides glycopeptides The pGP/AuG was successfully used for enriching and identifying glycopeptides from actual biological sample. 34649588 9 36 gly glycoprotein 2042:2053 arg1 glycoprotein yield glycoprotein yield Fterm glycoprotein Furthermore, it provides insight into strain engineering and bioprocess strategies, to improve glycoprotein yield and titre, and to avoid physiological burden of unfolded protein stress upon cell growth. 34227365 10 69 gly N-glycoproteins/N-glycopeptides 2095:2125 arg2 N-glycoproteins/N-glycopeptides N-glycoproteins/N-glycopeptides N-glycoproteins/N-glycopeptides On this basis, we analyzed the levels of N-glycoproteins/N-glycopeptides in urine samples. 33316563 6 15 gly glycopeptides 1274:1286 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides The prepared MOF particles possessed regular porosity, high surface area, good hydrophilicity, and offered an in-tip flow-based set-up enhanced the enrichment performance for N-linked glycopeptides. 33950562 4 12 gly glycosites 726:735 arg2 individual glycosites individual glycosites glycosites Target protein O-GlcNAcylation and de-O-GlcNAcylation complements methods to interrogate the role of O-GlcNAc on a global scale or at individual glycosites. 33597033 9 46 gly polysaccharides 1902:1916 arg1 decoupling lignin lignin polysaccharides Fterm lignin Oxidative enzymes such as laccases, peroxidases and lytic polysaccharide monooxygenases that are important for lignocellulose degradation in the terrestrial environment were present but not abundant, while a notable abundance of putative esterases (such as carbohydrate esterase family 1) associated with decoupling lignin from polysaccharides in lignocellulose was observed. 33597033 9 70 gly Oxidative 1574:1582 arg1 lytic polysaccharide monooxygenases Oxidative lytic polysaccharide monooxygenases Cterm Oxidative Oxidative enzymes such as laccases, peroxidases and lytic polysaccharide monooxygenases that are important for lignocellulose degradation in the terrestrial environment were present but not abundant, while a notable abundance of putative esterases (such as carbohydrate esterase family 1) associated with decoupling lignin from polysaccharides in lignocellulose was observed. 33134321 3 39 gly glycoprotein 413:424 arg1 a glycoprotein a glycoprotein Fterm glycoprotein One outstanding difference between torsinA and other AAA+ ATPases is that torsinA is a glycoprotein. 33134321 3 39 gly glycoprotein 413:424 arg1 torsinA torsinA PUBTATOR torsinA 1861 One outstanding difference between torsinA and other AAA+ ATPases is that torsinA is a glycoprotein. 34725712 4 0 part_of TPR 647:649 arg1 the TPR domain TPR the TPR domain OGER Site TPR P12270 domain Iterative truncation of the TPR domain of OGT maintains glycosyltransferase activity but alters subcellular localization of OGT in cells. 34725712 4 11 part_of OGT 661:663 arg1 the TPR domain OGT the TPR domain PUBTATOR Site OGT 8473 domain Iterative truncation of the TPR domain of OGT maintains glycosyltransferase activity but alters subcellular localization of OGT in cells. 33860037 2 78 part_of tRNA-derived 449:460 arg1 tRNA-derived small RNA fragment RNA tRNA-derived small RNA fragment Cterm Site RNA fragment METHODS tRNA derivative expression profiles in three pairs of HGSOC and adjacent normal ovarian tissues were conducted by tRNA-derived small RNA fragment (tRF) and tRNA half (tiRNA) sequencing. 33860037 2 89 part_of RNA 468:470 arg1 tRNA-derived small RNA fragment RNA tRNA-derived small RNA fragment Cterm Site RNA fragment METHODS tRNA derivative expression profiles in three pairs of HGSOC and adjacent normal ovarian tissues were conducted by tRNA-derived small RNA fragment (tRF) and tRNA half (tiRNA) sequencing. 34677046 1 61 gly attachment 306:315 arg2 N-sequon-containing membrane proteins AND heptasaccharide N-sequon-containing membrane proteins heptasaccharide Fterm proteins Campylobacter jejuni is a bacterial pathogen encoding a unique N-linked glycosylation (pgl) system that mediates attachment of a heptasaccharide to N-sequon-containing membrane proteins by the PglB oligosaccharyltransferase (OST). 33436086 5 64 gly glycosylation 1055:1067 arg2 site 158 site site Consistently, the HA-glycosylated H5N6 virus induced higher levels of inflammatory factors and resulted in stronger pathogenicity in mice than the virus without glycosylation at site 158. 33860037 0 18 part_of RNA-Derived 69:79 arg1 Transfer RNA-Derived Fragments Transfer RNA Transfer RNA-Derived Fragments Cterm Transfer RNA Differential Expression Profiles and Function Prediction of Transfer RNA-Derived Fragments in High-Grade Serous Ovarian Cancer. 33860037 0 57 part_of Transfer 60:67 arg1 Transfer RNA-Derived Fragments Transfer RNA Transfer RNA-Derived Fragments Cterm Transfer RNA Differential Expression Profiles and Function Prediction of Transfer RNA-Derived Fragments in High-Grade Serous Ovarian Cancer. 34335620 11 44 gly sites 2318:2322 arg1 GP - glycan cap sites GP - glycan cap sites Together these results reveal a restricted diversity of neutralizing humoral response in which mAbs targeting two antigenic sites on GP - glycan cap and base - play a principal role in plasma-antibody-mediated protective immunity against EVD. 34500222 4 19 gly glycopeptides 688:700 arg2 60 glycopeptides 60 glycopeptides glycopeptides Subsequently, 60 glycopeptides from complex biological sample (human saliva) were identified by Nano-LC-MS/MS. 32518069 6 17 part_of glycosites 1033:1042 arg1 synaptic proteins proteins glycosites Fterm Site proteins glycosites We identified 1965 formerly sialylated N-linked glycosites in synaptic proteins and found that the abundances of 430 glycosites changed after 5 s depolarization. 33733058 3 58 gly sialylated 779:788 arg1 sialylated receptors sialylated receptors Fterm receptors SARS-CoV-2 infects human cells through interaction with sialylated receptors at the surface of epithelial cells, such as angiotensin-converting enzyme 2 (ACE2). 33733058 3 58 gly sialylated 779:788 arg1 angiotensin-converting enzyme 2 angiotensin-converting enzyme 2 OGER angiotensin-converting enzyme 2 Q9BYF1 SARS-CoV-2 infects human cells through interaction with sialylated receptors at the surface of epithelial cells, such as angiotensin-converting enzyme 2 (ACE2). 33900328 10 50 gly N-glycosylation 1365:1379 arg1 FIP-glu FIP-glu OGER AminoAcid FIP Q15853 In conclusion, our findings revealed a novel mechanism by which N-glycosylation of FIP-glu improved its anti-inflammatory activity through the regulation of the expression of inflammatory cytokines in RAW264.7 via inhibition of p38 MAPK phosphorylation. 33292056 5 15 gly glycoproteins 1159:1171 arg1 N-linked glycans S glycoproteins N-linked glycans Cterm S glycoproteins 43740568 CBAs possess unique carbohydrate-binding specificity, therefore, CBAs like mannose-specific plant lectins/lectin-like mimic Pradimicin-A (PRM-A) can be used for targeting N-linked glycans of S glycoproteins. 33964586 10 3 gly G6PDH 1807:1811 arg1 O-GlcNAc modification G6PDH O-GlcNAc modification PUBTATOR G6PDH 24377 These findings, therefore, identified HA as a promising anti-I/R strategy for the heart and proposed O-GlcNAc modification of G6PDH as a therapeutic target in ischemic heart disease. 33964586 10 32 gly modification 1791:1802 arg3 G6PDH AND O-GlcNAc modification G6PDH O-GlcNAc modification PUBTATOR G6PDH 24377 These findings, therefore, identified HA as a promising anti-I/R strategy for the heart and proposed O-GlcNAc modification of G6PDH as a therapeutic target in ischemic heart disease. 32065175 0 37 gly glycopeptide 31:42 arg2 glycopeptide glycopeptide glycopeptide The effectiveness of filtering glycopeptide peak list files for Y ions. 32201074 10 3 part_of EGF 1278:1280 arg1 EGF domains EGF EGF domains OGER Site EGF P01133 domains Thus, O-GlcNAc on EGF domains possesses a shared function in the stability of EGF domains and Notch1 trafficking. 32201074 10 8 part_of EGF 1218:1220 arg1 EGF domains EGF EGF domains OGER Site EGF P01133 domains Thus, O-GlcNAc on EGF domains possesses a shared function in the stability of EGF domains and Notch1 trafficking. 32905539 5 33 gly glycosylation 653:665 arg1 p65 p65 OGER p65 Q9H4A3 In turn, GFPT2 enhanced the glycosylation of p65, which led to the nuclear translocation of p65 and then activated NF-κB pathway. 33197187 0 26 gly O-GlcNAcase 45:55 arg1 Nonsaccharide Inhibitors O-GlcNAcase Nonsaccharide Inhibitors PUBTATOR O-GlcNAcase 10724 Diazaspirononane Nonsaccharide Inhibitors of O-GlcNAcase (OGA) for the Treatment of Neurodegenerative Disorders. 32886756 1 14 gly residues 231:238 arg1 pseudaminic acid residues pseudaminic acid residues Some bacterial flagellins are O-glycosylated on surface-exposed serine/threonine residues with nonulosonic acids such as pseudaminic acid, legionaminic acid and their derivatives by flagellin nonulosonic acid glycosyltransferases, also called motility-associated factors (Maf). 32886756 1 14 gly residues 231:238 arg1 legionaminic acid residues legionaminic acid residues Some bacterial flagellins are O-glycosylated on surface-exposed serine/threonine residues with nonulosonic acids such as pseudaminic acid, legionaminic acid and their derivatives by flagellin nonulosonic acid glycosyltransferases, also called motility-associated factors (Maf). 32886756 1 29 gly O-glycosylated 180:193 arg2 surface-exposed serine/threonine residues residues residues Some bacterial flagellins are O-glycosylated on surface-exposed serine/threonine residues with nonulosonic acids such as pseudaminic acid, legionaminic acid and their derivatives by flagellin nonulosonic acid glycosyltransferases, also called motility-associated factors (Maf). 33270417 6 12 gly nonglycosylated 913:927 arg1 corresponding nonglycosylated peptides corresponding nonglycosylated peptides peptides Glycopeptides and corresponding nonglycosylated peptides were used to compare their deamidation rates. 33270417 6 39 gly used 943:946 arg2 Glycopeptides Glycopeptides Glycopeptides Glycopeptides and corresponding nonglycosylated peptides were used to compare their deamidation rates. 33270417 6 39 gly used 943:946 arg2 corresponding nonglycosylated peptides corresponding nonglycosylated peptides peptides Glycopeptides and corresponding nonglycosylated peptides were used to compare their deamidation rates. 32543352 9 40 gly proteins 1209:1216 arg1 potential protein- proteins potential protein- Fterm proteins Moreover, proteins with potential protein- and glycan-degrading domains could have an immune defence role or assist degrading adhesive mucus to facilitate the transition from stationary to locomotive states. 32543352 9 40 gly proteins 1209:1216 arg1 glycan-degrading domains proteins glycan-degrading domains Fterm proteins Moreover, proteins with potential protein- and glycan-degrading domains could have an immune defence role or assist degrading adhesive mucus to facilitate the transition from stationary to locomotive states. 32120884 0 54 part_of Mucin-Like 169:178 arg1 Mucin-Like Domains Mucin Mucin-Like Domains PUBTATOR Mucin 100508689 Proteomics Computational Analyses Suggest That the Envelope Glycoproteins of Segmented Jingmen Flavi-Like Viruses are Class II Viral Fusion Proteins (b-Penetrenes) with Mucin-Like Domains. 33335573 4 25 gly glycoprotein 578:589 arg1 Recombinant HEV pORF2 glycoprotein E1 Recombinant HEV pORF2 glycoprotein E1 Fterm glycoprotein Recombinant HEV pORF2 glycoprotein E1 (amino acids 439-617) and three mutant variants (N562L, N562C and N562K) were expressed in Pichia pastoris (P. pastoris) and SDS-PAGE, Western blot analysis, tunicamycin assay, double-antibody sandwich ELISA and in vitro PCR-based neutralization assay were performed to characterize the different constructs. 34363524 9 8 gly domain 1295:1300 arg1 -type zinc finger domains -type zinc finger domains domains Interestingly, protein-protein interaction analysis demonstrated some GLPs are associated with RAB6-interacting golgin, oligopeptide transmembrane transporter activity, beta-glucuronidase activity, protein N-linked glycosylation, multi-pass membrane protein, and proteins containing zinc finger (Znf) domain and RING (really interesting new gene)-type zinc finger domains. 33889548 8 79 gly glycopeptides 1348:1360 arg2 glycopeptides HPT glycopeptides PUBTATOR HPT Q9HD23 glycopeptides Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33889548 8 79 gly glycopeptides 1348:1360 arg2 glycopeptides PON1 glycopeptides PUBTATOR PON1 5444 glycopeptides Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33889548 8 79 gly glycopeptides 1348:1360 arg2 glycopeptides APOH glycopeptides PUBTATOR APOH 350 glycopeptides Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 32002580 3 96 part_of oxide-MOF 463:471 arg1 graphene oxide-MOF composite MOF graphene oxide-MOF composite OGER Site MOF Q9H7Z6 composite The fabrication of graphene oxide-MOF composite results in enhanced surface area with improved thermal and chemical stability. 32439055 6 10 gly glycopeptides 1132:1144 arg2 glycopeptides enrichment glycopeptides enrichment glycopeptides It also performed a high sensitivity (1 × 10-10 M HRP), a good enrichment capacity (250 mg/g) for glycopeptides enrichment and a high enrichment recovery of 78%, suggesting a great application prospect for the enrichment of N-linked glycopeptides and the analysis glycoproteomic research. 32439055 6 61 gly glycopeptides 1267:1279 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides It also performed a high sensitivity (1 × 10-10 M HRP), a good enrichment capacity (250 mg/g) for glycopeptides enrichment and a high enrichment recovery of 78%, suggesting a great application prospect for the enrichment of N-linked glycopeptides and the analysis glycoproteomic research. 34520755 4 58 gly N-glycosylation 832:846 arg2 the six N-glycosylation sites the six N-glycosylation sites sites Then the N-linked glycans released from the rHuPH20 by PNGase F were characterized by UPLC-FLR-MS, and the six N-glycosylation sites of the rHuPH20 were identified and characterized by UPLC-MS/MS at peptide levels. 33282554 2 5 gly proteins 300:307 arg1 C-mannosylation proteins C-mannosylation Fterm proteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 33282554 2 5 gly proteins 300:307 arg1 O-mannosylation proteins O-mannosylation Fterm proteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 33282554 2 36 gly C-mannosylation 361:375 arg1 proteins proteins Fterm proteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 33282554 2 36 gly C-mannosylation 361:375 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 33282554 2 40 gly O-mannosylation 344:358 arg1 proteins proteins Fterm proteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 33282554 2 40 gly O-mannosylation 344:358 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 33282554 2 86 gly glycoproteins 329:341 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins Dolichol phosphate mannose synthase (DPMS), an essential mannosyltransferase, plays a central role in post-translational modification of proteins, including N-linked glycoproteins, O-mannosylation, C-mannosylation and glycosylphosphatidylinositol anchors synthesis. 32931036 0 46 gly glycosylation 9:21 arg1 the mGlu7 receptor the mGlu7 receptor Fterm receptor N-linked glycosylation of the mGlu7 receptor regulates the forward trafficking and transsynaptic interaction with Elfn1. 32331895 1 59 part_of contain 256:262 arg1 Bovine peptide transporter 2 AND multiple putative N-glycosylation sites Bovine peptide transporter 2 multiple putative N-glycosylation sites OGER Site Bovine peptide transporter 2 Q16348 sites Bovine peptide transporter 2 (bPepT2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative N-glycosylation sites of asparagine residues. 32331895 1 59 part_of contain 256:262 arg1 Bovine peptide transporter 2 AND asparagine residues Bovine peptide transporter 2 asparagine residues OGER AminoAcid Bovine peptide transporter 2 Q16348 asparagine residues Bovine peptide transporter 2 (bPepT2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative N-glycosylation sites of asparagine residues. 32331895 1 59 part_of contain 256:262 arg1 bPepT2 AND multiple putative N-glycosylation sites bPepT2 multiple putative N-glycosylation sites Cterm Site bPepT2 Q16348 sites Bovine peptide transporter 2 (bPepT2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative N-glycosylation sites of asparagine residues. 32331895 1 59 part_of contain 256:262 arg1 bPepT2 AND asparagine residues bPepT2 asparagine residues Cterm AminoAcid bPepT2 Q16348 asparagine residues Bovine peptide transporter 2 (bPepT2), which mediates the absorption of di- and tripeptides in the bovine mammary gland, was predicted to contain multiple putative N-glycosylation sites of asparagine residues. 32295844 0 38 gly subunit 48:54 arg1 O-GlcNAcylation myosin phosphatase targeting subunit 1 O-GlcNAcylation PUBTATOR myosin phosphatase targeting subunit 1 4659 O-GlcNAcylation of myosin phosphatase targeting subunit 1 (MYPT1) dictates timely disjunction of centrosomes. 34155551 6 64 gly N-glycosylated 1139:1152 arg1 22 potential N-glycosylated sites 22 potential N-glycosylated sites sites We profiled 19 of its 22 potential N-glycosylated sites with 398 unique glycoforms using the dual-functional Ti-IMAC approach, which exhibited improvement of coverage by 1.6-fold compared to the conventional hydrophilic interaction chromatography (HILIC) glycopeptide enrichment method. 34155551 6 104 gly glycopeptide 1359:1370 arg2 the conventional hydrophilic interaction chromatography (HILIC) glycopeptide enrichment method the conventional hydrophilic interaction chromatography (HILIC) glycopeptide enrichment method glycopeptide We profiled 19 of its 22 potential N-glycosylated sites with 398 unique glycoforms using the dual-functional Ti-IMAC approach, which exhibited improvement of coverage by 1.6-fold compared to the conventional hydrophilic interaction chromatography (HILIC) glycopeptide enrichment method. 34306413 1 39 gly glycosylation 253:265 arg1 HIF-1α HIF-1α PUBTATOR HIF 3091 OBJECTIVE To investigate the correlation of the expression level of hypoxia-inducible factor-1α (HIF-1α) with the glycosylation of oral squamous cell carcinoma (OSCC). 34306413 1 39 gly glycosylation 253:265 arg1 hypoxia-inducible factor-1α hypoxia-inducible factor-1α PUBTATOR hypoxia-inducible factor-1α 3091 OBJECTIVE To investigate the correlation of the expression level of hypoxia-inducible factor-1α (HIF-1α) with the glycosylation of oral squamous cell carcinoma (OSCC). 33607086 2 11 part_of receptor-binding 289:304 arg1 the receptor-binding domain receptor the receptor-binding domain Fterm Site receptor domain Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (the virus causing COVID-19) is known to use the receptor-binding domain (RBD) at viral surface spike (S) protein to interact with the angiotensin-converting enzyme 2 (ACE2) receptor expressed on many human cell types. 33607086 2 74 part_of protein 346:352 arg1 the receptor-binding domain protein the receptor-binding domain Fterm Site protein domain Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (the virus causing COVID-19) is known to use the receptor-binding domain (RBD) at viral surface spike (S) protein to interact with the angiotensin-converting enzyme 2 (ACE2) receptor expressed on many human cell types. 31996435 8 34 gly glycosylation 1359:1371 arg2 the N-linked glycosylation motif the N-linked glycosylation motif motif While the mutation of the N-linked glycosylation motif (T168A) is primarily responsible for the formation of aggregates, other mutations within G1 that occurred earlier in the passage history of the Can strain also contribute to aggregation of the GPC within the ER.IMPORTANCE The development of vaccines and therapeutics to combat viral hemorrhagic fevers remains a top priority within the Implementation Plan of the U.S. Department of Health and Human Services Public Health Emergency Medical Countermeasures Enterprise. 33436086 6 93 gly glycosylation 1112:1124 arg2 site 158 site site However, H5N6 viruses without glycosylation at site 158 were more resistant to heat and bound host cells better than the HA-glycosylated viruses. 33889548 4 15 gly glycopeptides 612:624 arg2 serum N-linked intact glycopeptides serum N-linked intact glycopeptides glycopeptides Here, serum N-linked intact glycopeptides with molecular weight (MW) of 40-55 kDa were analyzed in a discovery set (n = 40) including AFP-negative HCC and liver cirrhosis (LC) patients using label-free quantification methodology. 34544801 1 73 gly glycosylation 106:118 arg1 IgG V regions IgG regions Cterm IgG regions Elevated N-linked glycosylation of IgG V regions (IgG-VN-Glyc) is an emerging molecular phenotype associated with autoimmune disorders. 33436086 8 71 gly glycosylation 1458:1470 arg1 HA HA site Cterm HA site These findings highlight the importance of glycosylation at site 158 of HA for the pathogenicity of the H5N6 viruses. 33436086 8 71 gly glycosylation 1458:1470 arg2 site 158 site site These findings highlight the importance of glycosylation at site 158 of HA for the pathogenicity of the H5N6 viruses. 33436086 8 71 gly glycosylation 1458:1470 arg2 site 158 HA site Cterm HA site These findings highlight the importance of glycosylation at site 158 of HA for the pathogenicity of the H5N6 viruses. 34711323 5 77 gly glycoforms 730:739 arg1 EPO glycoforms EPO glycoforms PUBTATOR EPO 2056 Alternatively, comprehensive insights into the glycoform heterogeneity of intact EPO are obtained using ESI MS-based methods with or without upfront separation of EPO glycoforms. 34711323 5 85 gly heterogeneity 620:632 arg1 intact EPO intact EPO PUBTATOR EPO 2056 Alternatively, comprehensive insights into the glycoform heterogeneity of intact EPO are obtained using ESI MS-based methods with or without upfront separation of EPO glycoforms. 33436086 8 87 part_of HA 1487:1488 arg1 site 158 HA site 158 Cterm Site HA site These findings highlight the importance of glycosylation at site 158 of HA for the pathogenicity of the H5N6 viruses. 33997889 6 55 gly glycosylated 1008:1019 arg1 an engineered carrier protein an engineered carrier protein Fterm protein In addition to ComP, we previously demonstrated that an engineered carrier protein containing a large fragment of ComP is also glycosylated by PglS. 34500222 2 39 gly glycopeptides 409:421 arg2 glycopeptides glycopeptides glycopeptides The combination attack with two different zwitterionic polymers resulted in enhanced adsorption sites for glycopeptides. 33624753 5 44 part_of fibronectin 855:865 arg1 fibronectin type III domains fibronectin fibronectin type III domains OGER Site fibronectin P02751 domains Choanoflagellate semaphorins (Sema-FN1) contain several domain features distinct from metazoan semaphorins, including an N-terminal Reeler domain that may facilitate dimer stabilization, an array of fibronectin type III domains, a variable serine/threonine-rich domain that is a potential site for O-linked glycosylation, and a SEA domain that can undergo autoproteolysis. 31859482 8 29 gly glycoprotein 1340:1351 arg1 fetuin fetuin Fterm fetuin Following development and optimization of the C18/MAX-Tip methodology using the standard glycoprotein, fetuin, we investigated normal urine samples to obtain N-linked glycoprotein information. 31859482 8 29 gly glycoprotein 1340:1351 arg1 the standard glycoprotein the standard glycoprotein Fterm glycoprotein Following development and optimization of the C18/MAX-Tip methodology using the standard glycoprotein, fetuin, we investigated normal urine samples to obtain N-linked glycoprotein information. 31859482 8 48 gly glycoprotein 1418:1429 arg1 N-linked glycoprotein information N-linked glycoprotein information Fterm glycoprotein Following development and optimization of the C18/MAX-Tip methodology using the standard glycoprotein, fetuin, we investigated normal urine samples to obtain N-linked glycoprotein information. 33806920 6 2 gly hypoglycosylated 915:930 arg1 The hypoglycosylated COX-2 The hypoglycosylated COX-2 PUBTATOR COX-2 5743 The hypoglycosylated COX-2 induced by SIN-1 showed a reduced capacity to generate prostaglandins and the activity was only partially recovered after immunoprecipitation. 34056367 10 110 gly glycosylation 1859:1871 arg2 the glycosylation site the glycosylation site site Glycosylation of the samples was performed using Escherichia coli-expressed NGT from the Actinobacillus pleuropneumoniae genome, and a single sugar moiety of glucose, provided from a nucleotide-linked donor, was added to the glycosylation site. 32423823 3 80 gly α-1,6-fucosylated 508:524 arg1 α-1,6-fucosylated oligosaccharides α-1,6-fucosylated oligosaccharides Among the many Futs, α-1,6-fucosyltransferase (Fut8) is the only enzyme that produces α-1,6-fucosylated oligosaccharides (core fucose). 32423823 3 80 gly α-1,6-fucosylated 508:524 arg1 core fucose core fucose Among the many Futs, α-1,6-fucosyltransferase (Fut8) is the only enzyme that produces α-1,6-fucosylated oligosaccharides (core fucose). 34335620 9 36 gly sites 1798:1802 arg1 the base region the base region region Molecular and functional analysis of proteomics-identified mAbs revealed recognition of epitopes in three major antigenic sites - the GP head domain, the glycan cap, and the base region, with a high prevalence of neutralizing and protective mAb specificities that targeted the base and glycan cap regions on the GP. 34290711 3 5 gly sialylation 647:657 arg1 cellular proteins cellular proteins Fterm proteins Recent studies suggest that modulation of ST6GAL1 (ST6 beta-galactoside alpha-2,6-sialyltransferase-1), which catalyzes terminal α2-6 sialylation of cellular proteins, may regulate inflammation and contribute to COPD phenotype(s). 34290711 3 85 gly proteins 671:678 arg1 terminal α2-6 sialylation proteins terminal α2-6 sialylation Fterm proteins Recent studies suggest that modulation of ST6GAL1 (ST6 beta-galactoside alpha-2,6-sialyltransferase-1), which catalyzes terminal α2-6 sialylation of cellular proteins, may regulate inflammation and contribute to COPD phenotype(s). 32275306 12 38 gly composition 1477:1487 arg1 xylose position xylose position Total cellular fatty acid composition on xylose presented some differences compared with that on glycerol. 34863021 6 21 gly removal 895:901 arg2 BDD FVIII AND N-linked glycans BDD FVIII N-linked glycans PUBTATOR FVIII 14069 RESULTS BDD FVIII-N41G, FVIII-N239A, FVIII-N1810A, and FVIII-N2118A with confirmed removal of N-linked glycans and similar glycosylation profiles to BDD FVIII were produced. 34693225 3 24 gly glycopeptide 326:337 arg2 an O-linked glycopeptide an O-linked glycopeptide glycopeptide Herein, we describe the discovery and characterization of an O-linked glycopeptide (Mg7a) as a major component of the venom of the ant Myrmecia gulosa. 32676596 5 50 gly glycoprotein 975:986 arg1 The respiratory syncytial virus fusion (F) glycoprotein The respiratory syncytial virus fusion (F) glycoprotein Fterm glycoprotein The respiratory syncytial virus fusion (F) glycoprotein trimer - stabilized in the prefusion conformation and fused with SpyCatcher - could be efficiently conjugated to LuS-SpyTag or ferritin-SpyTag, enabling multivalent display of F trimers with prefusion antigenicity. 34041222 7 119 gly contamination 1383:1395 arg1 the recovered lignin lignin contamination Fterm lignin Close examination of the isolated lignin showed that the main carbohydrate contamination in the recovered lignin was butyl-xyloside and other butoxylate carbohydrates. 32922663 9 71 gly N-glycosylation 1557:1571 arg1 CD44 CD44 OGER CD44 P16070 Further, we identified distinct N-glycosylation pattern of CD44, MGAM, and GINM1 between NMIBC and MIBC patients, which may be associated with disease progression in bladder cancer. 32922663 9 71 gly N-glycosylation 1557:1571 arg1 MGAM MGAM PUBTATOR MGAM 8972 Further, we identified distinct N-glycosylation pattern of CD44, MGAM, and GINM1 between NMIBC and MIBC patients, which may be associated with disease progression in bladder cancer. 32922663 9 71 gly N-glycosylation 1557:1571 arg1 GINM1 GINM1 PUBTATOR GINM1 Q9NU53 Further, we identified distinct N-glycosylation pattern of CD44, MGAM, and GINM1 between NMIBC and MIBC patients, which may be associated with disease progression in bladder cancer. 34677046 11 10 gly glycosites 1549:1558 arg2 C. jejuni glycosites C. jejuni glycosites glycosites There are now 166 experimentally verified C. jejuni glycosites and evidence for occupancy or nonoccupancy of 31 additional sites. 34677046 11 23 gly occupancy 1577:1585 arg2 31 additional sites 31 additional sites sites There are now 166 experimentally verified C. jejuni glycosites and evidence for occupancy or nonoccupancy of 31 additional sites. 33420984 6 38 gly sialylated 1361:1370 arg1 sialylated N-linked glycopeptides sialylated N-linked glycopeptides glycopeptides The protocol involves an initial titanium dioxide (TiO2) step to enrich for phosphopeptides and sialylated N-linked glycopeptides followed by glycan release and post-fractionation using sequential elution from immobilized metal affinity chromatography (SIMAC) to separate mono-phosphorylated and deglycosylated peptides from multi-phosphorylated ones. 33420984 6 45 gly glycopeptides 1381:1393 arg2 sialylated N-linked glycopeptides sialylated N-linked glycopeptides glycopeptides The protocol involves an initial titanium dioxide (TiO2) step to enrich for phosphopeptides and sialylated N-linked glycopeptides followed by glycan release and post-fractionation using sequential elution from immobilized metal affinity chromatography (SIMAC) to separate mono-phosphorylated and deglycosylated peptides from multi-phosphorylated ones. 33420984 6 65 gly deglycosylated 1561:1574 arg1 separate mono-phosphorylated and deglycosylated peptides separate mono-phosphorylated and deglycosylated peptides peptides The protocol involves an initial titanium dioxide (TiO2) step to enrich for phosphopeptides and sialylated N-linked glycopeptides followed by glycan release and post-fractionation using sequential elution from immobilized metal affinity chromatography (SIMAC) to separate mono-phosphorylated and deglycosylated peptides from multi-phosphorylated ones. 33118239 0 10 gly glycoprotein 87:98 arg1 glycoprotein synthesis glycoprotein synthesis Fterm glycoprotein Application of 2,2'-dipyridyl disulfide-mediated thiazolidine ring-opening reaction to glycoprotein synthesis: Total chemical synthesis of evasin-3. 34544801 1 18 part_of IgG 123:125 arg1 IgG V regions IgG V IgG V regions Cterm Site IgG V regions Elevated N-linked glycosylation of IgG V regions (IgG-VN-Glyc) is an emerging molecular phenotype associated with autoimmune disorders. 34544801 1 65 part_of V 127:127 arg1 IgG V regions IgG V IgG V regions Cterm Site IgG V regions Elevated N-linked glycosylation of IgG V regions (IgG-VN-Glyc) is an emerging molecular phenotype associated with autoimmune disorders. 32119511 5 21 gly domain 915:920 arg1 OGT OGT domain PUBTATOR OGT 8473 Truncation of the tetratricopeptide repeat domain as in OGT(4) increased selectivity for the target protein through the nanobody by reducing global elevation of O-GlcNAc levels in the cell. 34677046 3 28 gly glycosites 650:659 arg2 potential glycosites potential glycosites glycosites We exploited pglB-positive (wild-type; WT) and -negative (ΔpglB) proteomes to identify potential glycosites. 35006749 2 54 gly glycosylation 313:325 arg1 PD-L1 PD-L1 PUBTATOR PD-L1 29126 However, the recognition of PD-L1 by antibodies is usually suppressed due to the N-linked glycosylation of PD-L1. 34873473 2 46 part_of HGF 459:461 arg1 MET HGF receptor MET PUBTATOR AminoAcid HGF receptor 4233 MET In this study, we revealed that fucosyltransferase 8 (FUT8), an enzyme that mediates the core fucosylation of N-linked glycosylation, is an important regulator of malignant characteristics in human glioma that acts by modifying the activities of both the HGF receptor (MET) and epidermal growth factor receptor (EGFR). 34155551 5 91 gly glycopeptides 1036:1048 arg2 sialyl glycopeptides sialyl glycopeptides glycopeptides This strategy helps eliminate signal suppression from neutral glycopeptides for the detection of sialyl glycopeptides and improves the glycoform coverage of the S protein. 34155551 5 96 gly glycopeptides 994:1006 arg2 neutral glycopeptides neutral glycopeptides glycopeptides This strategy helps eliminate signal suppression from neutral glycopeptides for the detection of sialyl glycopeptides and improves the glycoform coverage of the S protein. 33889548 8 2 part_of HPT/HPTR 1373:1380 arg1 glycopeptides HPT glycopeptides PUBTATOR Site HPT Q9HD23 glycopeptides Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33889548 8 50 part_of PON1 1387:1390 arg1 glycopeptides PON1 glycopeptides PUBTATOR Site PON1 5444 glycopeptides Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33889548 8 88 part_of APOH 1367:1370 arg1 glycopeptides APOH glycopeptides PUBTATOR Site APOH 350 glycopeptides Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33877808 3 20 gly glycopeptides 656:668 arg2 intact glycopeptides intact glycopeptides glycopeptides Here, we propose a new enrichment strategy using a boronic acid (BA)-functionalized mesoporous graphene-silica composite (denoted as GO@mSiO2-GLYMO-APB) for isolating intact glycopeptides from complex biological samples. 33406421 1 0 gly moiety 179:184 arg1 target proteins proteins moiety Fterm proteins O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) is a unique enzyme introducing O-GlcNAc moiety on target proteins, and it critically regulates various cellular processes in diverse cell types. 34668190 12 71 gly p65 2039:2041 arg1 O-GlcNAcylation p65 O-GlcNAcylation PUBTATOR p65 19697 Taken together, our data suggested that O-GlcNAcylation of p65 enhanced p65 activity and nuclear translocation leading to the upregulation of IL-6, which maintained energy homeostasis and promotes cell survival in mouse skeletal muscle during cold exposure. 32408838 5 27 gly contains 341:348 arg1 The new saponin AND nine sugar units The new saponin nine sugar units Fterm saponin The new saponin contains nine sugar units with two O-linked sugar chains, which is relatively rare in natural products. 33849982 2 89 gly glycoproteins 362:374 arg1 glycoproteins glycoproteins Fterm glycoproteins This spectacular expansion is reflected in the dynamism and diversity of the fungal cell wall, a matrix of polysaccharides and glycoproteins pivotal to fungal life history strategies and a major target in the development of antifungal compounds. 33316563 2 83 gly glycopeptides 547:559 arg2 glycopeptides enrichment glycopeptides enrichment glycopeptides In this study, we synthesized the amine-functionalized titanium metal-organic framework (NH2-MIL-125(Ti)) by a simple hydrothermal method, characterized and used for glycopeptides enrichment. 33316563 1 9 gly glycopeptides 160:172 arg2 Endogenous glycopeptides Endogenous glycopeptides glycopeptides Endogenous glycopeptides are significantly important in diverse pathological and physiological systems, but their direct analysis is severely hampered by their low abundance and presence of interfering species in biological fluids. 34459483 2 33 gly sialylated 385:394 arg1 sialylated glycans sialylated glycans These species express "Siglec-like" adhesins that bind sialylated glycans on host glycoproteins, which can aid the formation of infected platelet-fibrin thrombi (vegetations) on cardiac valve surfaces. 34459483 2 47 gly glycans 396:402 arg1 host glycoproteins glycoproteins glycans Fterm glycoproteins These species express "Siglec-like" adhesins that bind sialylated glycans on host glycoproteins, which can aid the formation of infected platelet-fibrin thrombi (vegetations) on cardiac valve surfaces. 34459483 2 90 gly glycoproteins 412:424 arg1 host glycoproteins host glycoproteins Fterm glycoproteins These species express "Siglec-like" adhesins that bind sialylated glycans on host glycoproteins, which can aid the formation of infected platelet-fibrin thrombi (vegetations) on cardiac valve surfaces. 33682335 2 21 gly glycoforms 329:338 arg1 ribonuclease B ribonuclease B Cterm ribonuclease B Separations of the glycoforms of ribonuclease B compared to its released glycans were studied using a commercial hydrophilic interaction liquid chromatography column. 32002580 7 95 gly glycopeptide 1233:1244 arg2 N-linked glycopeptide enrichment N-linked glycopeptide enrichment glycopeptide GO@UiO-66-PBA nanoparticles show a low detection limit (1 fmol) and good specificity (1:200), reusability and reproducibility for N-linked glycopeptide enrichment from IgG digest. 33073165 7 46 gly glycosylated 1419:1430 arg1 glycosylated collagen sequences glycosylated collagen sequences sequences The Fmoc solid-phase strategy ultimately allowed for chemical biology approaches to be utilized to study tumor cell interactions with glycosylated collagen sequences and document the modulation of receptor interactions by Hyl posttranslational modification. 34227365 0 111 gly N-glycoproteins/N-glycopeptides 60:90 arg2 human urinary N-glycoproteins/N-glycopeptides human urinary N-glycoproteins/N-glycopeptides N-glycoproteins/N-glycopeptides [Large-scale enrichment and identification of human urinary N-glycoproteins/N-glycopeptides]. 33682335 6 61 gly glycoprotein 919:930 arg1 the glycan glycoprotein the glycan Fterm glycoprotein To investigate why, three-dimensional structures of the glycoprotein and the glycan were computed under chromatographic conditions. 34725712 2 73 gly glycosite 384:392 arg2 glycosite selection glycosite selection glycosite OGT and its isoforms select from these substrates through the tetratricopeptide repeat (TPR) domain, yet the impact of truncations to the TPR domain on substrate and glycosite selection is unresolved. 33390949 6 45 gly have 1098:1101 arg1 APS-Ⅲ AND monosaccharide residues APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33390949 6 45 gly have 1098:1101 arg1 APS-Ⅰ AND monosaccharide residues APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33390949 6 45 gly have 1098:1101 arg1 APS-Ⅰ AND monosaccharide residues APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33177197 10 57 gly glycosylated 1417:1428 arg1 a highly glycosylated protein a highly glycosylated protein Fterm protein Overall, the study shed light on the function of NLG of NA in virus replication and budding.IMPORTANCE NA is a highly glycosylated protein. 33024038 4 32 gly glycoproteins 649:661 arg1 factors V and XII factors V and XII Fterm factors V Previously, CpaA was shown to cleave two O-linked glycoproteins, factors V and XII, leading to reduced blood coagulation. 33024038 4 32 gly glycoproteins 649:661 arg1 two O-linked glycoproteins two O-linked glycoproteins Fterm glycoproteins Previously, CpaA was shown to cleave two O-linked glycoproteins, factors V and XII, leading to reduced blood coagulation. 33552834 1 11 gly glycosylated 267:278 arg1 glycosylated SARS-CoV-2 spike 2 protein glycosylated SARS-CoV-2 spike 2 protein PUBTATOR spike 2 protein 43740568 The pandemic COVID-19 was caused by a novel Coronavirus-2 (SARS-CoV-2) that infects humans through the binding of glycosylated SARS-CoV-2 spike 2 protein to the glycosylated ACE2 receptor. 33552834 1 55 gly glycosylated 314:325 arg1 the glycosylated ACE2 receptor the glycosylated ACE2 receptor PUBTATOR ACE2 receptor 59272 The pandemic COVID-19 was caused by a novel Coronavirus-2 (SARS-CoV-2) that infects humans through the binding of glycosylated SARS-CoV-2 spike 2 protein to the glycosylated ACE2 receptor. 32766576 8 91 gly glycan 1255:1260 arg1 Asn90 Asn90 Asn90 AminoAcid Asn90 SMD results indicate that CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 32120884 5 38 part_of mucin-like 988:997 arg1 an additional mucin-like domain mucin an additional mucin-like domain PUBTATOR Site mucin 100508689 domain Some of the glycoproteins are predicted to have an additional mucin-like domain located prior to the transmembrane anchor, whereas others are predicted to have a stem consisting of two alpha-helices. 32120884 5 36 part_of have 969:972 arg1 the glycoproteins AND an additional mucin-like domain the glycoproteins an additional mucin-like domain Fterm Site glycoproteins domain Some of the glycoproteins are predicted to have an additional mucin-like domain located prior to the transmembrane anchor, whereas others are predicted to have a stem consisting of two alpha-helices. 32120884 6 11 part_of glycoprotein 1247:1258 arg1 domains glycoprotein domains Fterm Site glycoprotein domains The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence. 32120884 6 11 part_of glycoprotein 1247:1258 arg1 a mucin-like domain glycoprotein a mucin-like domain Fterm Site glycoprotein domain The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence. 32120884 6 56 part_of mucin-like 1267:1276 arg1 a mucin-like domain mucin a mucin-like domain PUBTATOR Site mucin 100508689 domain The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence. 34195388 6 65 gly O-glycosylation 838:852 arg1 Fos Fos PUBTATOR Fos 14281 Specificity protein 1 (Sp1) expression and O-glycosylation, as well as the role of AP-1 members Jun and Fos in the regulation of GSTP expression, were also assessed. 34391905 6 82 gly residues 1613:1620 arg1 Cs1,6RhaT tryptophan residues Cs1,6RhaT tryptophan residues Functional analysis of substitution mutants for the glutamate and tryptophan residues in Cs1,6RhaT further support their role in determining sugar-sugar/branch-forming GT specificity. 33203757 9 75 gly factors 1851:1857 arg1 general glycan modifications factors general glycan modifications Fterm factors Glycoproteome-wide analysis of several bacterial pathogens has revealed general glycan modifications of virulence factors and protein assemblies. 34071374 5 23 gly HAp 981:983 arg1 polysaccharides HAp polysaccharides OGER HAp Q99933 Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. 33451024 3 6 part_of sites 537:541 arg1 HA proteins proteins sites Fterm Site proteins sites This study aims to identify the optimal DC-SIGN interacting N-glycosylation sites in HA proteins of H5N1-AIVs. 33451024 3 72 part_of DC-SIGN 501:507 arg1 the optimal DC-SIGN interacting N-glycosylation sites DC-SIGN the optimal DC-SIGN interacting N-glycosylation sites PUBTATOR Site DC-SIGN 30835 sites This study aims to identify the optimal DC-SIGN interacting N-glycosylation sites in HA proteins of H5N1-AIVs. 34069226 4 36 gly IgG 563:565 arg1 glycan-enhancing modifications IgG glycan-enhancing modifications Cterm IgG However, it is not yet clear if glycan-enhancing modifications of human IgG translate into more effective treatment in mouse models. 34734627 0 0 part_of STT3B-dependent 78:92 arg1 an STT3B-dependent acceptor site STT3B an STT3B-dependent acceptor site PUBTATOR Site STT3B 201595 site A cytosolic reductase pathway is required for efficient N-glycosylation of an STT3B-dependent acceptor site. 34322281 0 90 gly glycosyltransferase 56:74 arg1 glycosyltransferase GalNAc-T2 transferase glycosyltransferase GalNAc-T2 Fterm transferase Structural basis for peptide substrate specificities of glycosyltransferase GalNAc-T2. 34478712 7 35 gly glycoprotein 1210:1221 arg1 lysosomal glycoprotein p67 lysosomal glycoprotein p67 Fterm glycoprotein The latter bind a carbohydrate epitope present on lysosomal glycoprotein p67 that we show here consists of (-6Galβ1-4GlcNAcβ1-)≥4 poly-N-acetyllactosamine repeats. 34478712 7 68 gly present 1189:1195 arg1 lysosomal glycoprotein p67 AND a carbohydrate epitope lysosomal glycoprotein p67 a carbohydrate epitope OGER p67 P50579 The latter bind a carbohydrate epitope present on lysosomal glycoprotein p67 that we show here consists of (-6Galβ1-4GlcNAcβ1-)≥4 poly-N-acetyllactosamine repeats. 34215103 1 19 gly glycopeptide 154:165 arg2 endogenous glycopeptide endogenous glycopeptide glycopeptide It has been confirmed that endogenous glycopeptide plays an important role in a variety of pathological and physiological processes. 32892442 9 12 gly Akt 1577:1579 arg1 the O-GlcNAcylation Akt the O-GlcNAcylation PUBTATOR Akt 207 Moreover, OSMI-1 further promoted HBV replication by inducing autophagosome formation via inhibiting the O-GlcNAcylation of Akt and mTOR. 32892442 9 35 gly mTOR 1585:1588 arg1 the O-GlcNAcylation mTOR the O-GlcNAcylation PUBTATOR mTOR 2475 Moreover, OSMI-1 further promoted HBV replication by inducing autophagosome formation via inhibiting the O-GlcNAcylation of Akt and mTOR. 34839131 7 40 gly agrocin 1054:1060 arg1 the corresponding phosphorylated glucose-2-phosphoramidate component agrocin the corresponding phosphorylated glucose-2-phosphoramidate component Fterm agrocin Data suggests that the open chain aldehyde forms of the 2-phosphorylated opines agrocinopine C and agrocinopine A and the corresponding phosphorylated glucose-2-phosphoramidate component of the antibiotic agrocin 84 play a central role in agrocin's selective toxicity to certain strains of Agrobacterium after uptake via Ti plasmid-encoded permeases. 32401558 6 32 part_of ECM 1121:1123 arg1 the composition ECM the composition OGER Site ECM Q13201 position Collectively, these results have revealed for the first time the composition of the ECM of the biofilms formed by Scedosporium/Lomentospora species and the role it plays in their architecture. 33608772 9 88 part_of IgA1 1425:1428 arg1 the heavy constant region IgA1 the heavy constant region PUBTATOR Site IgA1 P01876 region Analyses by ion trap mass spectrometry using collision-induced dissociation and electron-transfer dissociation modes revealed one O-linked N-acetylhexosamine modification site at Ser268 located in the heavy constant region of IgA1; unfortunately, it cannot be discriminated whether it was N-acetylglucosamine or N-acetylgalactosamine because of their identical molecular mass. 32766576 8 35 part_of ACE2 1274:1277 arg1 Asn90 ACE2 Asn90 PUBTATOR AminoAcid ACE2 59272 Asn90 SMD results indicate that CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 32522876 0 48 gly glycosylation 21:33 arg1 chromatin factors chromatin factors Fterm factors Methylation-directed glycosylation of chromatin factors represses retrotransposon promoters. 32665404 7 45 part_of IgG1 1192:1195 arg1 the bovine IgG1 hinge region IgG1 the bovine IgG1 hinge region OGER Site IgG1 P01857 region Infliximab was modified to incorporate the sequences from these regions, including the bovine IgG1 hinge region and a predicted disulfide bonding motif linking the upper hinge region, the CH1 domain, and the light chain. 32665404 7 60 part_of regions 1162:1168 arg1 the sequences regions the sequences sequences Infliximab was modified to incorporate the sequences from these regions, including the bovine IgG1 hinge region and a predicted disulfide bonding motif linking the upper hinge region, the CH1 domain, and the light chain. 32665404 7 65 part_of CH1 1286:1288 arg1 the CH1 domain CH1 the CH1 domain OGER Site CH1 Q9UBS9 domain Infliximab was modified to incorporate the sequences from these regions, including the bovine IgG1 hinge region and a predicted disulfide bonding motif linking the upper hinge region, the CH1 domain, and the light chain. 33950562 3 43 gly O-GlcNAc 448:455 arg1 a target protein protein O-GlcNAc Fterm protein We recently reported a pair of methods to selectively install or remove O-GlcNAc on a target protein in cells using an engineered O-GlcNAc transferase (OGT) or split O-GlcNAcase (OGA) fused to a nanobody. 33809195 1 2 part_of proteins 245:252 arg1 Aberrant composition proteins Aberrant composition Fterm Site proteins position Aberrant composition of glycans in the tumor microenvironment (TME) and abnormal expression of extracellular matrix proteins are hallmarks of hepatocellular carcinoma (HCC); however, the mechanisms responsible for establishing the TME remain unclear. 34455356 10 61 gly glycosylation 2056:2068 arg1 DTMUV NS1 NS1 sites OGER NS1 Q9Y6Y0 sites Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 61 gly glycosylation 2056:2068 arg2 N175 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 61 gly glycosylation 2056:2068 arg2 N207 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 61 gly glycosylation 2056:2068 arg2 N130 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 61 gly glycosylation 2056:2068 arg2 N207 N130, N175 and N207 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 61 gly glycosylation 2056:2068 arg2 N130 N130, N175 and N207 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 61 gly glycosylation 2056:2068 arg2 N130 N130, N175 and N207 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34152647 5 53 gly Demannosylation 672:686 arg1 N-linked oligosaccharides N-linked oligosaccharides Demannosylation of N-linked oligosaccharides targets terminally misfolded proteins for ERAD. 33964586 8 85 gly enzyme 1163:1168 arg1 the pentose phosphate pathway enzyme the pentose phosphate pathway Fterm enzyme Hyperglycosylation activated glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme in the pentose phosphate pathway, resulting in an upregulation of NADPH/NADP+ and GSH/GSSG couples and enhancement of redox homeostasis in the heart. 34401531 9 68 part_of gut 1565:1567 arg1 the gut microbial composition gut the gut microbial composition PUBTATOR Site gut 110006 position Curdlan supplementation elevated fecal bile acid and SCFA production, improved host metabolic functions by altering the gut microbial composition in mice. 33557755 10 80 gly glycoproteins 1672:1684 arg1 other highly variable glycoproteins other highly variable glycoproteins Fterm glycoproteins As well as this application to HIV, our method can be used for other highly variable glycoproteins such as hepatitis C virus envelope. 34641490 7 40 part_of Pyrin 1791:1795 arg1 Pyrin Domain Family, Pyrin Domain Containing Protein 3 Pyrin Domain PUBTATOR Family, Pyrin Domain Containing Protein 3 Q12988 Accordingly, LDS might increase the expression of TREM2/DNAX-activating protein of 12 kDa (DAP12) and suppress the Toll-like receptor SX4 (TLR4) pathway and Recombinant NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)/cysteinyl aspartate specific proteinase-1 (Caspase-1) inflammasome expression by LDS in LPS-induced BV2 microglial cells. 34641490 7 76 part_of TLR4 1749:1752 arg1 Pyrin Domain SX4 (TLR4 Pyrin Domain PUBTATOR SX4 (TLR4 21898 Accordingly, LDS might increase the expression of TREM2/DNAX-activating protein of 12 kDa (DAP12) and suppress the Toll-like receptor SX4 (TLR4) pathway and Recombinant NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)/cysteinyl aspartate specific proteinase-1 (Caspase-1) inflammasome expression by LDS in LPS-induced BV2 microglial cells. 34641490 7 104 part_of SX4 1744:1746 arg1 Pyrin Domain SX4 (TLR4 Pyrin Domain PUBTATOR SX4 (TLR4 21898 Accordingly, LDS might increase the expression of TREM2/DNAX-activating protein of 12 kDa (DAP12) and suppress the Toll-like receptor SX4 (TLR4) pathway and Recombinant NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)/cysteinyl aspartate specific proteinase-1 (Caspase-1) inflammasome expression by LDS in LPS-induced BV2 microglial cells. 33292056 6 13 gly glycoprotein 1314:1325 arg1 the S glycoprotein glycans the S glycoprotein glycans Here, we report studies on the binding and stability of lectins (NPA, UDA, GRFT, CV-N and wild-type and mutant BanLec) and PRM-A with the S glycoprotein glycans via docking and MD simulation. 35499042 16 70 part_of AQP3 2143:2146 arg1 the AQP3 promoter region AQP3 the AQP3 promoter region PUBTATOR Site AQP3 360 region Furthermore, the transcription factor SP1 specifically bound to the AQP3 promoter region, and O-GlcNAcylation of SP1 increased its stability and transcriptional regulation of AQP3 which is associated with O-GlcNAcylation of SP1. 32991074 3 70 gly proteins 473:480 arg1 Aberrant O-GlcNAcylation proteins Aberrant O-GlcNAcylation Fterm proteins Aberrant O-GlcNAcylation of proteins is implicated in signaling in metabolic diseases such as diabetes and cancer. 32065175 4 30 gly O-glycopeptide 763:776 arg2 O-glycopeptide data O-glycopeptide data O-glycopeptide The strategy proved to be extremely powerful for O-glycopeptide data, and also of benefit for N-linked data, where it allowed rescue of low confidence results from database searching. 32885501 0 53 gly glycosylated 33:44 arg1 a highly glycosylated fusion protein a highly glycosylated fusion protein Fterm protein Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip-based capillary electrophoresis-mass spectrometry. 34227365 23 7 gly N-glycoproteins/N-glycopeptides 3839:3869 arg2 the N-glycoproteins/N-glycopeptides the N-glycoproteins/N-glycopeptides N-glycoproteins/N-glycopeptides To investigate the biological processes and functions of these proteins, gene ontology (GO) analysis was performed on the N-glycoproteins/N-glycopeptides differentially expressed between males and females. 34262722 11 114 gly mannose 1740:1746 arg1 BLC-1 BLC mannose OGER BLC O43927 The proportions of glucose, mannose in BLC-1 and BLC-2 were 47.68%, 39.18%, and 76.59%, 6.86%, respectively. 34262722 11 130 gly glucose 1731:1737 arg1 BLC-1 BLC glucose OGER BLC O43927 The proportions of glucose, mannose in BLC-1 and BLC-2 were 47.68%, 39.18%, and 76.59%, 6.86%, respectively. 32542517 11 7 gly carrying 1415:1422 arg1 proteins AND Neu5Gc-determinants proteins Neu5Gc-determinants Fterm proteins In contrast to the restricted number of proteins carrying α-Gal and LacdiNAc, the distribution of proteins carrying Neu5Gc-determinants varied between species and between different tissues of the same species. 32542517 11 65 gly carrying 1357:1364 arg1 proteins AND α-Gal proteins α-Gal Fterm proteins In contrast to the restricted number of proteins carrying α-Gal and LacdiNAc, the distribution of proteins carrying Neu5Gc-determinants varied between species and between different tissues of the same species. 32542517 11 65 gly carrying 1357:1364 arg1 proteins AND LacdiNAc proteins LacdiNAc Fterm proteins In contrast to the restricted number of proteins carrying α-Gal and LacdiNAc, the distribution of proteins carrying Neu5Gc-determinants varied between species and between different tissues of the same species. 32264694 8 82 part_of TSHR 768:771 arg1 all the deduced TSHR amino acid sequences TSHR all the deduced TSHR amino acid sequences OGER Site TSHR P16473 sequences Bioinformatics analysis indicated that all the deduced TSHR amino acid sequences contained seven putative transmembrane domains, and the TSHR-3 protein lacked one potential N-linked glycosylation site (N-E-S) compared to the other three deduced proteins. 32264694 8 4 part_of contained 794:802 arg1 all the deduced TSHR amino acid sequences AND seven putative transmembrane domains all the deduced TSHR amino acid sequences seven putative transmembrane domains domains Bioinformatics analysis indicated that all the deduced TSHR amino acid sequences contained seven putative transmembrane domains, and the TSHR-3 protein lacked one potential N-linked glycosylation site (N-E-S) compared to the other three deduced proteins. 33906120 5 11 gly glycoproteins 710:722 arg1 The identified glycoproteins The identified glycoproteins Fterm glycoproteins The identified glycoproteins were mainly concentrated in plasma membranes (25.6%), cell wall (16.8%) and extracellular area (16%). 32120884 6 32 gly glycoproteins 1182:1194 arg1 Jingmen flavi-virus glycoproteins Jingmen flavi-virus glycoproteins Fterm glycoproteins The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence. 32120884 6 67 gly glycoprotein 1247:1258 arg1 a common class II precursor glycoprotein a common class II precursor glycoprotein Fterm glycoprotein The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence. 34925382 7 17 part_of HLA 1137:1139 arg1 the O-GlcNAcylated HLA peptides HLA the O-GlcNAcylated HLA peptides OGER Site HLA peptides Most of the O-GlcNAcylated HLA peptides originate from nuclear proteins, notably transcription factors. 31981375 0 19 gly glycoprotein 76:87 arg1 a glycoprotein a glycoprotein Fterm glycoprotein Extraction, purification, characterization, and rheological properties of a glycoprotein from Cynomorium songaricum Rupr. 33167279 6 56 gly His-tag 1072:1078 arg1 then the His-tag residue part His then the His-tag residue part His The immobilized peptide would be cleavaged by proteinase K, then the His-tag residue part will leave the surface of Au film, resulting less His-tag could bind to Ni2+ and a small SPR signal would be record. 32640409 8 6 gly glycoproteins 1022:1034 arg1 glycoproteins glycoproteins Fterm glycoproteins They contain proteins and glycoproteins or possibly polysaccharides without detectable amounts of lipids. 34215103 2 70 gly glycopeptide 288:299 arg2 endogenous glycopeptide endogenous glycopeptide glycopeptide However, direct analysis of endogenous glycopeptide is still a great challenge owing to the low abundance of endogenous glycopeptides and the presence of a large number of interfering substances such as large-sized proteins and heteropeptides in complex biological sample. 34215103 2 106 gly glycopeptides 369:381 arg2 endogenous glycopeptides endogenous glycopeptides glycopeptides However, direct analysis of endogenous glycopeptide is still a great challenge owing to the low abundance of endogenous glycopeptides and the presence of a large number of interfering substances such as large-sized proteins and heteropeptides in complex biological sample. 33552834 5 66 gly glycosylation 1183:1195 arg2 more than one N-linked glycosylation site more than one N-linked glycosylation site site Another striking observation is the absence of N-glycosylation site Asn103 in all mammals and many species, lack more than one N-linked glycosylation site in the ACE2 receptor. 33552834 5 103 gly N-glycosylation 1094:1108 arg2 N-glycosylation site Asn103 site Asn103 site Asn103 Another striking observation is the absence of N-glycosylation site Asn103 in all mammals and many species, lack more than one N-linked glycosylation site in the ACE2 receptor. 34489534 4 38 gly glycosylated 751:762 arg1 the fully glycosylated mature Piezo1 protein the fully glycosylated mature Piezo1 protein PUBTATOR Piezo1 protein 9780 Both mutation of these asparagines (N2294Q/N2331Q) and treatment with an enzyme that hydrolyses N-linked oligosaccharides (PNGaseF) eliminates the fully glycosylated mature Piezo1 protein. 32117864 5 15 gly proteins 1015:1022 arg1 1,3,4-O-Bu3ManNAc-treated cells proteins 1,3,4-O-Bu3ManNAc-treated cells Fterm proteins Gene ontology (GO) analysis showed that flux-based changes to sialylation were broadly distributed across classes of proteins in 1,3,4-O-Bu3ManNAc-treated cells. 33906120 10 2 part_of kinase 1843:1848 arg1 the glycosylation modification and glycosylation sites kinase the glycosylation modification and glycosylation sites Fterm Site kinase sites PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 33906120 10 15 part_of exohydrolase 1878:1889 arg1 the glycosylation modification and glycosylation sites exohydrolase the glycosylation modification and glycosylation sites Fterm Site exohydrolase sites PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 34042420 8 31 gly peptides 1042:1049 arg1 N- N- Gradient elution produced fractions enriched in peptides with phosphate, mannose-6-phosphate, and N- and O-linked glycans. 34042420 8 31 gly peptides 1042:1049 arg1 mannose-6-phosphate mannose-6-phosphate Gradient elution produced fractions enriched in peptides with phosphate, mannose-6-phosphate, and N- and O-linked glycans. 33802415 2 50 gly contain 324:330 arg1 FSHβ subunits AND N-linked oligosaccharides FSHβ subunits two sites N-linked oligosaccharides Fterm Site subunits sites Both α and FSHβ subunits contain two sites of N-linked oligosaccharides, which are important for its function. 34155551 2 105 gly glycoprotein 407:418 arg1 the spike (S) glycoprotein the spike (S) glycoprotein Fterm glycoprotein The development of effective vaccines has been focusing on the spike (S) glycoprotein, which mediates viral invasion to human cells through its interaction with the angiotensin-converting enzyme 2 (ACE2) receptor. 34304571 4 69 part_of EWS 754:756 arg1 the EWS N-terminal low complexity region EWS the EWS N-terminal low complexity region PUBTATOR Site EWS 2130 region Using an in vitro approach, we found that O-GlcNAcylation reduces the phase separation propensity of the EWS N-terminal low complexity region (LCRN) under different conditions, including in the presence of the arginine- and glycine-rich RNA-binding domains (RBD). 32315666 3 101 part_of % 838:838 arg1 (3) Remineralizing composite 30% NACP (3) Remineralizing composite OGER Site 30% NACP P37840 composite METHODS Five groups were tested: (1) Heliomolar nanocomposite (Commercial control); (2) Experimental composite control (0% NACP, 0% DMAHDM); (3) Remineralizing composite (30% NACP); (4) Antibacterial composite (3% DMAHDM); (5) Remineralizing and antibacterial composite (NACP + DMAHDM). 33547363 6 40 gly glycosylated 1155:1166 arg1 118 N-linked glycosylated peptides 118 N-linked glycosylated peptides peptides Solid-phase extraction of N-linked glycosylated peptides (SPEG) revealed 118 N-linked glycosylated peptides from cells grown in high- and low-iron growth conditions. 33547363 6 46 gly glycosylated 1104:1115 arg1 N-linked glycosylated peptides SPEG peptides OGER SPEG Q15772 peptides Solid-phase extraction of N-linked glycosylated peptides (SPEG) revealed 118 N-linked glycosylated peptides from cells grown in high- and low-iron growth conditions. 34524799 5 40 gly glycoproteins 742:754 arg1 glycoproteins glycoproteins Fterm glycoproteins Significantly, PRM-EA is sensitive to glycoproteins carrying high mannose-type and hybrid-type N-linked glycans, but not to those carrying complex-type N-linked glycans. 34524799 5 47 gly carrying 756:763 arg1 glycoproteins AND high mannose-type and hybrid-type N-linked glycans glycoproteins high mannose-type and hybrid-type N-linked glycans Fterm glycoproteins Significantly, PRM-EA is sensitive to glycoproteins carrying high mannose-type and hybrid-type N-linked glycans, but not to those carrying complex-type N-linked glycans. 34524799 5 47 gly carrying 756:763 arg1 glycoproteins AND mannose-type glycoproteins mannose-type Fterm glycoproteins Significantly, PRM-EA is sensitive to glycoproteins carrying high mannose-type and hybrid-type N-linked glycans, but not to those carrying complex-type N-linked glycans. 32931036 9 18 gly N-glycosylation 1230:1244 arg1 mGlu7 mGlu7 Cterm mGlu7 2917 We find that N-glycosylation of mGlu7 promotes its interaction with Elfn1, thereby enabling proper localization and stable surface expression of mGlu7 at the presynaptic active zone. 33301684 2 0 gly glycosylation 263:275 arg1 this protein this protein Fterm protein It is associated with the progression of kidney diseases; therefore, changes in the glycosylation profile of this protein could serve as a potential biomarker for kidney health. 33788254 9 6 gly glycoprotein 1469:1480 arg1 glycoprotein productions glycoprotein productions Fterm glycoprotein The present study demonstrates that BN model can be a powerful tool in upstream process and medium development for glycoprotein productions. 33335573 0 0 part_of proteins 68:75 arg1 position 562 proteins position 562 Fterm Site proteins position 562 Different mutations at position 562 of the hepatitis E virus capsid proteins exhibit differential effects on viral neutralizing activity. 34069226 9 44 gly afucosylated 1336:1347 arg1 afucosylated therapeutic IgG afucosylated therapeutic IgG Cterm IgG These results confirm the potential of using afucosylated therapeutic IgG to increase their efficacy. 32922663 0 88 gly N-glycosylation 120:134 arg1 MGAM MGAM PUBTATOR MGAM 8972 Urinary glycoproteomic profiling of non-muscle invasive and muscle invasive bladder carcinoma patients reveals distinct N-glycosylation pattern of CD44, MGAM, and GINM1. 32922663 0 88 gly N-glycosylation 120:134 arg1 GINM1 GINM1 OGER GINM1 Q9NU53 Urinary glycoproteomic profiling of non-muscle invasive and muscle invasive bladder carcinoma patients reveals distinct N-glycosylation pattern of CD44, MGAM, and GINM1. 32922663 0 88 gly N-glycosylation 120:134 arg1 CD44 CD44 OGER CD44 P16070 Urinary glycoproteomic profiling of non-muscle invasive and muscle invasive bladder carcinoma patients reveals distinct N-glycosylation pattern of CD44, MGAM, and GINM1. 34058199 6 8 part_of domain 1047:1052 arg1 two putative active sites domain two putative active sites sites B3GLCT has two putative active sites, one in the N-terminal region and the other in the C-terminal glycosyltransferase domain. 34058199 6 57 part_of has 935:937 arg1 B3GLCT AND two putative active sites B3GLCT two putative active sites PUBTATOR Site B3GLCT 145173 sites B3GLCT has two putative active sites, one in the N-terminal region and the other in the C-terminal glycosyltransferase domain. 33877808 5 42 gly glycopeptide 1244:1255 arg2 intact glycopeptide analysis intact glycopeptide analysis glycopeptide The results from the enrichment performance of the strategy evaluated by standard glycoproteins and the application to global N- and O-glycosylation analyses in human serum indicate the robustness and potential of the strategy for intact glycopeptide analysis. 33877808 5 78 gly glycoproteins 1088:1100 arg1 standard glycoproteins standard glycoproteins Fterm glycoproteins The results from the enrichment performance of the strategy evaluated by standard glycoproteins and the application to global N- and O-glycosylation analyses in human serum indicate the robustness and potential of the strategy for intact glycopeptide analysis. 32946675 10 39 gly homeostasis 1265:1275 arg1 dams dams homeostasis OGER dams Q9Y6J3 In conclusion, WTS exposure during lactation altered the milk composition and altered lipid profile, glucose homeostasis and hormonal levels in dams and pups. 33728790 7 33 gly N-glycosylation 1577:1591 arg2 multiple N-glycosylation motifs multiple N-glycosylation motifs motifs In addition to providing a map of the oomycete N-glycoproteome, this work confirms that P. sojae has evolved multiple N-glycosylation motifs essential for growth. 34155551 4 30 gly glycoprotein 900:911 arg1 a recombinant SARS-CoV-2 S glycoprotein a recombinant SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 We explore the novel use of dual-functionalized titanium (IV)-immobilized metal affinity chromatography (Ti-IMAC) material for simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293 cells. 34155551 4 7 gly glycopeptides 856:868 arg1 a recombinant SARS-CoV-2 S glycoprotein S glycoprotein glycopeptides PUBTATOR S glycoprotein 43740568 glycopeptides We explore the novel use of dual-functionalized titanium (IV)-immobilized metal affinity chromatography (Ti-IMAC) material for simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293 cells. 32922663 6 36 gly N-glycopeptides 1083:1097 arg2 635 N-glycopeptides 635 N-glycopeptides N-glycopeptides We identified 635 N-glycopeptides corresponding to 381 proteins and 543 N-glycopeptides corresponding to 326 proteins in NMIBC and MIBC patients respectively. 32922663 6 81 gly N-glycopeptides 1137:1151 arg2 543 N-glycopeptides 543 N-glycopeptides N-glycopeptides We identified 635 N-glycopeptides corresponding to 381 proteins and 543 N-glycopeptides corresponding to 326 proteins in NMIBC and MIBC patients respectively. 33557755 5 37 gly glycosylation 800:812 arg2 statistically conserved global sites sites sites RESULTS We developed an automated library building tool NGlyAlign, that organizes similar N-linked glycosylation sites as block constraints and statistically conserved global sites as single site constraints to automatically enforce partial columns in consistency-based MSA methods such as Dialign. 33888690 8 4 gly glycoprotein 1182:1193 arg1 on-demand glycoprotein production on-demand glycoprotein production Fterm glycoprotein We anticipate that these methods will facilitate on-demand glycoprotein production and enable new CFE systems with membrane-associated activities. 32337418 1 27 gly glycosylation 112:124 arg1 immunoglobulin G immunoglobulin G domain PUBTATOR immunoglobulin G 16059 domain N-Linked glycosylation of the fragment crystallizable (Fc) domain of immunoglobulin G (IgG) is considered a significant modulator of antibody functions, which is known to be subclass-specific. 34284575 3 58 gly glycosylation 341:353 arg1 this protein this protein Fterm protein A good knowledge of the glycosylation profile of this protein is key to successful vaccine development. 33806146 3 12 gly contained 431:439 arg1 AAP AND polysaccharides AAP polysaccharides PUBTATOR AAP 5345 AAP contained high amounts of polysaccharides (349.83 ± 5.00 mg/g extract) with a molecular weight of 158 kDa. 34811775 4 81 part_of epitopes 787:794 arg1 the immunodominant VP7 and VP4 proteins proteins epitopes Fterm Site proteins epitopes Full-genome analyses of the pathogenic G12P[11] strain was carried out to map the genotype constellation and further to explore the variations in the antigenic epitopes on the immunodominant VP7 and VP4 proteins, the amino acid sequences were compared with neonatal strains; ROTAVAC® (G9P[11]) and asymptomatic G12P[11] and also other G/P-type matched strains. 33167279 5 63 gly used 952:955 arg2 six histidine six histidine histidine The designed peptide contains three recognition sites, one is the cleavage site of protease K, one is the O-GlcNAcylated site by OGT, and another is six histidine which be used as the signal report probe to recognize Ni2+. 34625868 4 65 gly glycosylation 795:807 arg1 these closely related AIV sequences these closely related AIV sequences sequences Furthermore, N-linked glycosylation analysis of these closely related AIV sequences supported the possibility of multiple spillover events of highly pathogenic H5N1 and low pathogenic H9N2 viruses from various avian species to swine. 34455356 6 94 gly glycosylation 1236:1248 arg1 DTMUV NS1 NS1 sites OGER NS1 Q9Y6Y0 sites The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 34455356 6 94 gly glycosylation 1236:1248 arg2 N207 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 34455356 6 94 gly glycosylation 1236:1248 arg2 N175 NS1 N130, N175 and N207 OGER NS1 Q9Y6Y0 N130, N175 and N207 The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 34455356 6 94 gly glycosylation 1236:1248 arg2 N175 N130, N175 and N207 N130, N175 and N207 The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 33728790 4 17 gly glycosites 790:799 arg2 496 glycosites 496 glycosites glycosites A total of 355 N-glycosylated proteins was found, containing 496 glycosites, potentially involved in glycan degradation, carbon metabolism, glycolysis, or other metabolic pathways. 33728790 4 61 gly 355 N-glycosylated 736:753 arg1 355 N-glycosylated proteins 355 N-glycosylated proteins Fterm proteins A total of 355 N-glycosylated proteins was found, containing 496 glycosites, potentially involved in glycan degradation, carbon metabolism, glycolysis, or other metabolic pathways. 32033138 2 53 part_of chitosan 338:345 arg1 different compositions chitosan different compositions PUBTATOR Site chitosan 474403 positions In this study, a series of blend films composed of chitosan (CS)/poly(allylamine hydrochloride) (PAH) in different compositions were prepared as smart drug delivery matrices. 32946675 2 3 part_of composition 282:292 arg1 dams dams composition OGER Site dams Q9Y6J3 position, Herein, the effects of waterpipe tobacco smoke (WTS) exposure during lactation on milk composition, hormonal levels and biochemical profile in dams and pups were investigated. 33586851 4 27 part_of GPI 600:602 arg1 GPI fragments GPI GPI fragments OGER Site GPI P06744 fragments We synthesized a series of GPI fragments and evaluated the interactions and arrangement of these glycolipids in monolayers as a 2-D membrane model. 34934932 2 0 gly N-acetylglucosamine 307:325 arg1 motif motif motif Hexosamine biosynthesis pathway (HBP), an accessory pathway of glycolysis, is known to be involved in the attachment of O-linked N-acetylglucosamine motif (O-GlcNAcylation) to proteins, a post-translational modification. 34934932 2 33 gly attachment 284:293 arg1 proteins AND O-linked N-acetylglucosamine proteins O-linked N-acetylglucosamine Fterm proteins Hexosamine biosynthesis pathway (HBP), an accessory pathway of glycolysis, is known to be involved in the attachment of O-linked N-acetylglucosamine motif (O-GlcNAcylation) to proteins, a post-translational modification. 34401531 0 78 part_of gut 23:25 arg1 gut microbial composition gut gut microbial composition PUBTATOR Site gut 110006 position, Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice. 34367145 8 40 gly glycoproteins 1615:1627 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins Next, lectin blot and deglycosylation assay were done, suggesting the key role of N-linked glycoproteins in exofree. 32352119 10 3 gly face 1831:1834 arg1 cellular nucleotide-sugar concentrations face cellular nucleotide-sugar concentrations face Thus, the glycosylation system output exhibits 'robustness' even in the face of significant changes in cellular nucleotide-sugar concentrations. 32352119 10 93 gly concentrations 1888:1901 arg1 the face the face the face Site face Thus, the glycosylation system output exhibits 'robustness' even in the face of significant changes in cellular nucleotide-sugar concentrations. 34418081 4 0 gly glycosylation 642:654 arg2 three glycosylation sites three glycosylation sites sites Inhibition of N-linked glycosylation by introducing mutations in three glycosylation sites inhibited AZU1 loading into SEVs. 33370382 5 6 gly N88 925:927 arg1 conserved N156 and N160 glycans N88 conserved N156 and N160 glycans N88 Analyses of site-specific glycans revealed that SP swapping altered Env glycan content and occupancy on multiple N-linked glycosites, including conserved N156 and N160 glycans in the V1V2 region at the Env trimer apex and N88 at the trimer base. 33370382 5 35 gly glycosites 825:834 arg2 multiple N-linked glycosites multiple N-linked glycosites glycosites Analyses of site-specific glycans revealed that SP swapping altered Env glycan content and occupancy on multiple N-linked glycosites, including conserved N156 and N160 glycans in the V1V2 region at the Env trimer apex and N88 at the trimer base. 33370382 5 39 gly glycans 871:877 arg1 the V1V2 region the V1V2 region the V1V2 region Site region Analyses of site-specific glycans revealed that SP swapping altered Env glycan content and occupancy on multiple N-linked glycosites, including conserved N156 and N160 glycans in the V1V2 region at the Env trimer apex and N88 at the trimer base. 33370382 5 39 gly glycans 871:877 arg1 N88 N88 N88 SpecificSite N88 Analyses of site-specific glycans revealed that SP swapping altered Env glycan content and occupancy on multiple N-linked glycosites, including conserved N156 and N160 glycans in the V1V2 region at the Env trimer apex and N88 at the trimer base. 34408013 5 16 part_of C3 771:772 arg1 C3 deposition C1q binding, C4, C3 C3 deposition PUBTATOR Site C1q binding, C4, C3 720 position, In this study, we show that galactose incorporated in the IgG1-Fc enhances C1q binding, C4, C3 deposition, and complement-dependent cellular cytotoxicity in human erythrocytes and Raji cells. 33981677 9 49 gly glycopeptide 1722:1733 arg2 glycopeptide deglycosylation glycopeptide deglycosylation glycopeptide Taking advantage of the unique homogeneous reaction of a TMF-immobilized enzyme, both protein digestion and glycopeptide deglycosylation can be finished within 3 min, and the whole sample processing time can be reduced by more than 20 times. 33024038 7 11 gly serine 1128:1133 arg1 residue residue residue We show that CpaA has a unique consensus target sequence that consists of a glycosylated serine or threonine residue after a proline residue (P-S/T), and its activity is not affected by sialic acids. 34401710 5 78 part_of SAA 936:938 arg1 particular SAA residues SAA particular SAA residues OGER Site SAA P0DJI8 residues In our work we aimed to apply diverse computational tools to characterize SAA-GAG complexes formation and to answer questions about their potential specificity, energetic patterns, particular SAA residues involved in these interactions, favourable oligomeric state of the protein and the potential influence of GAGs on SAA aggregation. 34401710 5 82 part_of protein 1016:1022 arg1 particular SAA residues protein particular SAA residues Fterm Site protein residues In our work we aimed to apply diverse computational tools to characterize SAA-GAG complexes formation and to answer questions about their potential specificity, energetic patterns, particular SAA residues involved in these interactions, favourable oligomeric state of the protein and the potential influence of GAGs on SAA aggregation. 34677046 4 38 gly glycopeptides 697:709 arg2 known glycopeptides known glycopeptides glycopeptides The nonglycosylated forms of known glycopeptides were typically increased in protein normalized abundance in ΔpglB relative to WT and restored by pglB reintroduction (ΔpglB::pglB). 34677046 4 43 gly nonglycosylated 666:680 arg1 known glycopeptides known glycopeptides glycopeptides The nonglycosylated forms of known glycopeptides were typically increased in protein normalized abundance in ΔpglB relative to WT and restored by pglB reintroduction (ΔpglB::pglB). 34120284 9 67 gly glycoproteins 1208:1220 arg1 misfolded glycoproteins misfolded glycoproteins Fterm glycoproteins Our results suggest that TiO2NPs exposure increases the levels of misfolded glycoproteins, resulting in generation of considerable amounts of free N-glycans. 32910282 3 10 gly residues 667:674 arg1 intracellular serine/threonine residues intracellular serine/threonine residues residues Intracellular O-linked glycosylation (O-GlcNAcylation), which is the result of two enzymes that dynamically add and remove single N-acetylglucosamines to and from intracellular serine/threonine residues (OGT and OGA respectively), has proven to be an increasingly important post-translational modification that contributes to the regulation of many physiologic processes. 33168911 11 39 gly contained 1630:1638 arg1 eNOS AND novel O-GlcNAcylation sites eNOS novel O-GlcNAcylation sites PUBTATOR eNOS 4846 A marked increase in O-GlcNAcylation indicated that eNOS contained novel O-GlcNAcylation sites that were activated by glucose deprivation. 34734627 5 58 gly hypoglycosylated 828:843 arg1 a hypoglycosylated acceptor site a hypoglycosylated acceptor site site We identified a hypoglycosylated acceptor site that is adjacent to a cysteine involved in a short-range disulfide. 33272761 3 40 gly glycoprotein 293:304 arg1 an O-linked glycoprotein an O-linked glycoprotein Fterm glycoprotein Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 40 gly glycoprotein 293:304 arg1 Patatin Patatin PUBTATOR Patatin 102577633 Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 38 gly contained 311:319 arg1 an O-linked glycoprotein AND fucose monosaccharides an O-linked glycoprotein fucose monosaccharides Fterm glycoprotein Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 38 gly contained 311:319 arg1 an O-linked glycoprotein AND mannose an O-linked glycoprotein mannose Fterm glycoprotein Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 38 gly contained 311:319 arg1 Patatin AND fucose monosaccharides Patatin fucose monosaccharides PUBTATOR Patatin 102577633 Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 38 gly contained 311:319 arg1 Patatin AND mannose Patatin mannose PUBTATOR Patatin 102577633 Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 34858763 4 34 gly glycoproteins 540:552 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins NGLY1 catalyzes protein deglycosylation by cleaving the -aspartyl glycosylamine bond of N-linked glycoproteins and is thereby a component of the endoplasmic reticulum-associated degradation pathway. 32543352 8 69 gly glycoproteins 1135:1147 arg1 hemolectin hemolectin Fterm hemolectin Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. 32543352 8 69 gly glycoproteins 1135:1147 arg1 fibrous gel-forming glycoproteins fibrous gel-forming glycoproteins Fterm glycoproteins Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. 32543352 8 69 gly glycoproteins 1135:1147 arg1 fibrillin fibrillin Fterm fibrillin Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. 32543352 8 69 gly glycoproteins 1135:1147 arg1 SCO-spondin SCO-spondin PUBTATOR SCO-spondin 420367 Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. 33523898 6 23 gly glycoprotein 1194:1205 arg1 ER glycoprotein quality control ER glycoprotein quality control PUBTATOR ER glycoprotein 2069 The fidelity of N-glycosylation is not static in both nontransformed and tumor cells, and the TRAP complex regulates ER glycoprotein quality control under conditions of stress. 32264694 8 67 gly glycosylation 895:907 arg2 one potential N-linked glycosylation site one potential N-linked glycosylation site site Bioinformatics analysis indicated that all the deduced TSHR amino acid sequences contained seven putative transmembrane domains, and the TSHR-3 protein lacked one potential N-linked glycosylation site (N-E-S) compared to the other three deduced proteins. 32987930 8 9 gly glycosylation 1354:1366 arg2 the N-linked glycosylation site the N-linked glycosylation site site In conclusion, the substitution in the N-linked glycosylation site in E2 attenuated SAV3 in cell culture. 34105348 3 93 gly OGA 677:679 arg1 O-GlcNAc antibody CTD110.6 OGA O-GlcNAc antibody CTD110.6 OGER OGA Q9EQQ9 Here, we compared glycan array data of the mutant of Clostridium perfringen OGA (CpOGAD298N), O-GlcNAc antibody CTD110.6, and several lectins. 34625868 2 89 part_of hemagglutinin 379:391 arg1 full-length hemagglutinin (HA) sequences hemagglutinin full-length hemagglutinin (HA) sequences Fterm Site hemagglutinin sequences To unravel the transmission dynamics and spillover events of AIVs from avian reservoirs to swine, full-length hemagglutinin (HA) sequences of AIV subtypes (n = 11) reported from various avian species and swine were retrieved from the 'Influenza Research Database'. 33316563 10 44 gly used 1934:1937 arg2 glycopeptides glycopeptides glycopeptides These results demonstrated the excellent potential of NH2-MIL-125(Ti) based affinity tip for glycopeptides which can be used to enrich trace glycopeptide biomarkers from the biological fluids. 33316563 10 77 gly glycopeptides 1907:1919 arg2 glycopeptides glycopeptides glycopeptides These results demonstrated the excellent potential of NH2-MIL-125(Ti) based affinity tip for glycopeptides which can be used to enrich trace glycopeptide biomarkers from the biological fluids. 33316563 10 93 gly glycopeptide 1955:1966 arg2 trace glycopeptide biomarkers trace glycopeptide biomarkers glycopeptide These results demonstrated the excellent potential of NH2-MIL-125(Ti) based affinity tip for glycopeptides which can be used to enrich trace glycopeptide biomarkers from the biological fluids. 33232205 3 53 gly glycoprotein 851:862 arg1 the spike (S) glycoprotein the spike (S) glycoprotein Fterm glycoprotein By using a panel of rabbit antisera against virions and five structural proteins together with a convalescent serum, the spike (S) glycoprotein was shown to be N-linked glycosylated, PNGase F-sensitive, endoglycosidase H-resistant and cleaved by Furin-like proteases into S1 and S2 subunits. 33232205 3 71 gly glycosylated 889:900 arg1 the spike (S) glycoprotein the spike (S) glycoprotein Fterm glycoprotein By using a panel of rabbit antisera against virions and five structural proteins together with a convalescent serum, the spike (S) glycoprotein was shown to be N-linked glycosylated, PNGase F-sensitive, endoglycosidase H-resistant and cleaved by Furin-like proteases into S1 and S2 subunits. 33886622 7 72 gly glycoproteins 1147:1159 arg1 oligosaccharides glycoproteins oligosaccharides Fterm glycoproteins Incubation of purified PmCOX with endoglycosidase H, which cleaves oligosaccharides from N-linked glycoproteins, reduced the molecular mass of PmCOX. 32987930 5 40 gly glycosylation 768:780 arg2 the glycosylation motif the glycosylation motif motif Mutation of the glycosylation motif in E1 gave a complete inactivation of the virus as no viral replication could be detected in cell culture and infectious particles could not be rescued. 33806146 4 7 gly AAP 568:570 arg1 The main sugar composition AAP The main sugar composition PUBTATOR AAP 5345 The main sugar composition of AAP includes mannose, galactose, and glucose. 32931036 6 42 gly N-glycosylation 880:894 arg1 mGlu7 mGlu7 Cterm mGlu7 2917 We demonstrate that N-glycosylation is essential for forward transport and surface expression of mGlu7. 33607086 8 47 part_of ACE2 1352:1355 arg1 Asn90 ACE2 Asn90 PUBTATOR AminoAcid ACE2 59272 Asn90 SMD results indicate that SARS-CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 32776095 2 41 gly glycoproteins 373:385 arg1 EGFR EGFR PUBTATOR EGFR 1956 Mucin-type O-linked glycosylation is not limited to mucins and occurs on many cell surface glycoproteins including EGFR, where the number of sites can be limited. 32776095 2 41 gly glycoproteins 373:385 arg1 many cell surface glycoproteins many cell surface glycoproteins Fterm glycoproteins Mucin-type O-linked glycosylation is not limited to mucins and occurs on many cell surface glycoproteins including EGFR, where the number of sites can be limited. 33024038 9 49 gly glycoproteins 1554:1566 arg1 human glycoproteins human glycoproteins Fterm glycoproteins Similar bacterial glycoproteases have recently gained attention as tools for proteomic analysis of human glycoproteins, and CpaA appears to be a robust and attractive new component of the glycoproteomics toolbox. 32737203 5 69 part_of lipoprotein 921:931 arg1 lipoprotein deposition lipoprotein lipoprotein deposition Fterm Site lipoprotein position We hypothesize that this interaction promotes lipoprotein deposition onto BrM glycosaminoglycans, initiating downstream effects that contribute to RPE dysfunction/death. 32120884 2 59 part_of have 445:448 arg1 Jingmen flavi-like virus glycoproteins AND an ectodomain Jingmen flavi-like virus glycoproteins an ectodomain Fterm Site glycoproteins ectodomain Computational analyses were used to predict that Jingmen flavi-like virus glycoproteins have structural features of class II viral fusion proteins, including an ectodomain consisting of beta-sheets and short alpha-helices, a fusion peptide with interfacial hydrophobicity and a three-domain architecture. 34489534 3 18 gly glycosylation 417:429 arg1 the 'cap' region region region Here we show that N-linked glycosylation of two highly conserved asparagine residues in the 'cap' region of mechanosensitive Piezo1 channels are necessary for the mature protein to reach the plasma membrane. 34489534 3 19 gly glycosylation 417:429 arg1 two highly conserved asparagine residues two highly conserved asparagine residues asparagine residues Here we show that N-linked glycosylation of two highly conserved asparagine residues in the 'cap' region of mechanosensitive Piezo1 channels are necessary for the mature protein to reach the plasma membrane. 34155551 7 17 gly glycosylation 1419:1431 arg2 O-linked glycosylation site O-linked glycosylation site site We also identified O-linked glycosylation site that was not found using the conventional HILIC approach. 32922663 4 83 gly N-glycosylation 661:675 arg1 proteins proteins Fterm proteins In this study, we investigated the aberrant changes in N-glycosylation pattern of proteins associated with tumorigenesis as well as disease progression in bladder cancer. 32114385 8 63 gly O-GlcNAc 1081:1088 arg1 IKKβ IKKβ O-GlcNAc PUBTATOR IKKβ 1147 Mutations of IKKβ (S733A) and p65 (T352A) abrogated the O-GlcNAc in IKKβ and NF-κB and inhibited AIEC LF82-induced activation of NF-κB. 32114385 8 63 gly O-GlcNAc 1081:1088 arg1 NF-κB NF-κB O-GlcNAc PUBTATOR NF-κB 4790 Mutations of IKKβ (S733A) and p65 (T352A) abrogated the O-GlcNAc in IKKβ and NF-κB and inhibited AIEC LF82-induced activation of NF-κB. 32518069 6 35 gly glycosites 1102:1111 arg2 430 glycosites 430 glycosites glycosites We identified 1965 formerly sialylated N-linked glycosites in synaptic proteins and found that the abundances of 430 glycosites changed after 5 s depolarization. 32518069 6 59 gly glycosites 1033:1042 arg2 1965 formerly sialylated N-linked glycosites 1965 formerly sialylated N-linked glycosites glycosites We identified 1965 formerly sialylated N-linked glycosites in synaptic proteins and found that the abundances of 430 glycosites changed after 5 s depolarization. 32518069 6 67 gly sialylated 1013:1022 arg1 1965 formerly sialylated N-linked glycosites 1965 formerly sialylated N-linked glycosites glycosites We identified 1965 formerly sialylated N-linked glycosites in synaptic proteins and found that the abundances of 430 glycosites changed after 5 s depolarization. 33924774 6 74 gly presence 797:804 arg1 N81 AND complex glycans N81 complex glycans domain, and N81 In our study, tandem mass spectrometry analysis of recombinant ANTXR1-Fc revealed the presence of complex glycans at N166, N184 in the vWA domain, and N81 in the Fc domain. 33924774 6 74 gly presence 797:804 arg1 N184 AND complex glycans N166, N184 complex glycans N166, N184 In our study, tandem mass spectrometry analysis of recombinant ANTXR1-Fc revealed the presence of complex glycans at N166, N184 in the vWA domain, and N81 in the Fc domain. 32393178 7 90 gly glycosylation 1301:1313 arg2 the three N-linked glycosylation sites the three N-linked glycosylation sites sites All of the three N-linked glycosylation sites (i.e. sequons NCSV at HA 54, NHTV at HA 125, and NLSK at HA 160) identified by MTL-SGL to determine antigenic changes were experimentally validated in the H1N1 antigenic variants using mass spectrometry analyses. 32393178 7 110 gly sites 1315:1319 arg1 the three N-linked glycosylation sites the three N-linked glycosylation sites sites All of the three N-linked glycosylation sites (i.e. sequons NCSV at HA 54, NHTV at HA 125, and NLSK at HA 160) identified by MTL-SGL to determine antigenic changes were experimentally validated in the H1N1 antigenic variants using mass spectrometry analyses. 34026840 5 64 gly glycosylation 797:809 arg2 Two putative N-linked glycosylation motifs Two putative N-linked glycosylation motifs motifs Two putative N-linked glycosylation motifs and two cysteine residues could potentially form intramolecular disulfide bond present in HdhCA II. 32511336 2 43 gly glycoprotein 284:295 arg1 the spike (S) glycoprotein the spike (S) glycoprotein Fterm glycoprotein Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, that mediates cell entry and membrane fusion. 32185163 1 0 gly fucosylated 190:200 arg1 antennary fucosylated glycans antennary fucosylated glycans Changes in the abundance of antennary fucosylated glycans in human total plasma N-glycome (TPNG) have been associated with several diseases ranging from diabetes to various forms of cancer. 32518939 0 38 part_of EGF-like 11:18 arg1 The single EGF-like domain EGF The single EGF-like domain OGER Site EGF P01132 domain The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 32518939 0 54 part_of PAMR1 36:40 arg1 The single EGF-like domain PAMR1 The single EGF-like domain PUBTATOR Site PAMR1 210622 domain The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 32012886 2 5 gly modified 359:366 arg1 PrPC AND two N-linked glycans PrPC two N-linked glycans PUBTATOR PrPC 19122 PrPC is posttranslationally modified with two N-linked glycans. 32633815 9 13 part_of SARS-CoV-2 1190:1199 arg1 Those SARS-CoV-2 strains sequences SARS Those SARS-CoV-2 strains sequences OGER Site SARS P49591 sequences Those SARS-CoV-2 strains sequences were 100% identical by each other, significantly varied in RaTG13 and pangolin-CoV. 32108991 1 18 part_of factor 198:203 arg1 the A1A2A3 domains factor the A1A2A3 domains Fterm Site factor domains BACKGROUND Previous studies have demonstrated that the A1A2A3 domains of von Willebrand factor (VWF) play a key role in regulating macrophage-mediated clearance in vivo. 34636569 8 30 gly glycoproteins 1649:1661 arg1 missing glycoproteins missing glycoproteins Fterm glycoproteins The mirror-cutting digestion strategy also has great application potential in the exploration of missing glycoproteins from other complex samples to provide rich resources for glycobiology research. 34717991 1 12 gly used 188:191 arg2 lignocellulosic residues lignocellulosic residues residues Tropical agriculture produces large amounts of lignocellulosic residues that can potentially be used as a natural source of value-added products. 33924503 1 32 gly glycoproteins 174:186 arg1 the carbohydrate side chains glycoproteins the carbohydrate side chains Fterm glycoproteins Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). 32697003 4 68 gly glycoproteins 602:614 arg1 human and pig serum glycoproteins human and pig serum glycoproteins Fterm glycoproteins To validate the α-fucose phenotype observed previously we compared lectin affinity toward human and pig serum glycoproteins by dot blot analysis and confocal microscopy. 32046000 1 61 gly glycoprotein 139:150 arg1 The BclA3 glycoprotein The BclA3 glycoprotein Fterm glycoprotein Abstract: The BclA3 glycoprotein is a major component of the exosporangial layer of Clostridium difficile spores and in this study we demonstrate that this glycoprotein is a major spore surface associated antigen. 32046000 1 66 gly glycoprotein 275:286 arg1 this glycoprotein this glycoprotein Fterm glycoprotein Abstract: The BclA3 glycoprotein is a major component of the exosporangial layer of Clostridium difficile spores and in this study we demonstrate that this glycoprotein is a major spore surface associated antigen. 32516600 2 14 gly glycopeptides 426:438 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides We show that this transformation can be harnessed to generate amino acid building blocks including FmocAsn(GlcNAc)OH (1), a residue that has been previously shown to be a competent reagent in the solid-phase peptide synthesis of N-linked glycopeptides. 34863021 3 102 gly glycosylation 399:411 arg1 FVIII immunity FVIII immunity PUBTATOR FVIII 2157 However, knowledge of the role of FVIII asparagine (N)-linked glycosylation in FVIII immunity is limited. 34490144 0 85 gly Glycosylation 34:46 arg1 YghJ YghJ PUBTATOR YghJ 2847716 Linking inherent O-Linked Protein Glycosylation of YghJ to Increased Antigen Potential. 34805099 4 1 part_of motif 569:573 arg1 the side chain chain motif OGER Site chain P01308 motif Structurally, compound 1 features a novel dioxabicyclo[2.2.2]octan-3-one motif in the side chain. 34579295 5 25 gly residue 1138:1144 arg1 polysaccharides residue polysaccharides residue Furthermore, the extracts were fully characterized by complementary infrared and Raman spectroscopy, revealing the presence of phenolic acids, polysaccharides, sugars, and triterpenoids in the pulp, high content of unsaturated fatty acids in the seed oil, and significant amounts of phenolics and carotenoids in the seed residue. 34579295 5 25 gly residue 1138:1144 arg1 sugars residue sugars residue Furthermore, the extracts were fully characterized by complementary infrared and Raman spectroscopy, revealing the presence of phenolic acids, polysaccharides, sugars, and triterpenoids in the pulp, high content of unsaturated fatty acids in the seed oil, and significant amounts of phenolics and carotenoids in the seed residue. 31981375 3 5 gly glycoprotein 401:412 arg1 a maximum glycoprotein yield a maximum glycoprotein yield Fterm glycoprotein The results show that a maximum glycoprotein yield of 6.39 ± 0.32% was achieved at a ratio of solid to liquid 32:1 for 4.2 H at 52 °C. Then, the IR, monosaccharide composition, amino acid composition, type of glycopeptide linkage, and average molecular weight of CSG-1 purified from CSG were characterized. 31981375 3 17 gly glycopeptide 578:589 arg2 glycopeptide linkage glycopeptide linkage glycopeptide The results show that a maximum glycoprotein yield of 6.39 ± 0.32% was achieved at a ratio of solid to liquid 32:1 for 4.2 H at 52 °C. Then, the IR, monosaccharide composition, amino acid composition, type of glycopeptide linkage, and average molecular weight of CSG-1 purified from CSG were characterized. 34524799 4 7 gly glycoproteins 611:623 arg1 glycoproteins glycoproteins Fterm glycoproteins We also show that PRM-EA stains glycoproteins in dot blot assays, whereas PRM-A fails to do so, owing to severe aggregation. 32328881 2 13 part_of sequence 516:523 arg1 the side-chain side-chain sequence Fterm Site side-chain sequence In the central reaction, a pre-assembled high-mannose sugar is transferred from a lipid-linked donor substrate to the side-chain of an asparagine residue in an -N-X-T/S- sequence (where X is any residue except proline). 32328881 2 25 part_of side-chain 464:473 arg1 an -N-X-T/S- sequence side-chain an -N-X-T/S- sequence Fterm Site side-chain sequence In the central reaction, a pre-assembled high-mannose sugar is transferred from a lipid-linked donor substrate to the side-chain of an asparagine residue in an -N-X-T/S- sequence (where X is any residue except proline). 34373697 2 55 gly present 329:335 arg2 Harms AND The polysaccharides Harms The polysaccharides OGER Harms Q9ULH0 The polysaccharides present in A. giraldii Harms contain major bioactive substances, which have antioxidant, anticancer, and antiviral activities. 33282554 1 26 gly glycosylation 103:115 arg1 proteins proteins Fterm proteins BACKGROUND Altered glycosylation of proteins contributes to tumor progression. 34347113 4 62 gly glycoproteins 1051:1063 arg1 fetuin fetuin Fterm fetuin To address this challenge, nanoflow liquid chromatography, ion mobility spectrometry, and mass spectrometry (nLC-IMS-MS) were combined with the Individuality Normalization when Labeling with the Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy to study a series of glycan standards and those enzymatically released from the glycoproteins horseradish peroxidase, fetuin, and pooled human plasma. 34347113 4 62 gly glycoproteins 1051:1063 arg1 horseradish peroxidase horseradish peroxidase Fterm peroxidase To address this challenge, nanoflow liquid chromatography, ion mobility spectrometry, and mass spectrometry (nLC-IMS-MS) were combined with the Individuality Normalization when Labeling with the Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy to study a series of glycan standards and those enzymatically released from the glycoproteins horseradish peroxidase, fetuin, and pooled human plasma. 34347113 4 62 gly glycoproteins 1051:1063 arg1 the glycoproteins horseradish peroxidase, fetuin, and pooled human plasma the glycoproteins horseradish peroxidase, fetuin, and pooled human plasma Fterm glycoproteins To address this challenge, nanoflow liquid chromatography, ion mobility spectrometry, and mass spectrometry (nLC-IMS-MS) were combined with the Individuality Normalization when Labeling with the Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy to study a series of glycan standards and those enzymatically released from the glycoproteins horseradish peroxidase, fetuin, and pooled human plasma. 33361116 4 34 gly afucosylated 482:493 arg1 afucosylated IgG afucosylated IgG Cterm IgG Here, we report that afucosylated IgG (approximately 6% of total IgG in humans) are specifically formed against enveloped viruses but generally not against other antigens. 32910214 8 25 gly deglycosylated 1161:1174 arg1 the enriched and PNGase-treated deglycosylated peptides the enriched and PNGase-treated deglycosylated peptides peptides Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. 32910214 8 52 gly glycopeptides 1048:1060 arg2 Total 318 N-linked glycopeptides Total 318 N-linked glycopeptides glycopeptides Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. 32892942 8 8 gly N-glycosites 1179:1190 arg2 344 unique N-glycosites 344 unique N-glycosites N-glycosites Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 32892942 8 18 gly 172 N-glycoproteins 1227:1245 arg1 172 N-glycoproteins 172 N-glycoproteins Fterm 172 N-glycoproteins Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 32892942 8 42 gly N-glycopeptides 1334:1348 arg2 825 intact N-glycopeptides 825 intact N-glycopeptides N-glycopeptides Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 32892942 8 56 gly N-glycopeptides 1425:1439 arg2 the N-glycopeptides the N-glycopeptides N-glycopeptides Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 32892942 8 15 gly N-glycopeptides 1206:1220 arg2 598 unique N-glycopeptides 172 N-glycoproteins N-glycopeptides Fterm 172 N-glycoproteins N-glycopeptides Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 μL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. 33167279 5 23 part_of K 872:872 arg1 the cleavage site protease K the cleavage site Cterm Site protease K site The designed peptide contains three recognition sites, one is the cleavage site of protease K, one is the O-GlcNAcylated site by OGT, and another is six histidine which be used as the signal report probe to recognize Ni2+. 33167279 5 82 part_of contains 801:808 arg1 The designed peptide AND three recognition sites The designed peptide three recognition sites sites The designed peptide contains three recognition sites, one is the cleavage site of protease K, one is the O-GlcNAcylated site by OGT, and another is six histidine which be used as the signal report probe to recognize Ni2+. 33997889 6 29 part_of ComP 995:998 arg1 a large fragment ComP a large fragment OGER Site ComP P49747 fragment In addition to ComP, we previously demonstrated that an engineered carrier protein containing a large fragment of ComP is also glycosylated by PglS. 33997889 6 60 part_of containing 964:973 arg1 an engineered carrier protein AND a large fragment an engineered carrier protein a large fragment Fterm Site protein fragment In addition to ComP, we previously demonstrated that an engineered carrier protein containing a large fragment of ComP is also glycosylated by PglS. 31904283 11 5 gly O-glycoproteins 1645:1659 arg1 mucin-type O-glycoproteins mucin-type O-glycoproteins Fterm O-glycoproteins This suggests a cell nonautonomous mechanism for mucin-type O-glycoproteins in maintaining podocyte function. 32878894 5 31 gly sites 1303:1307 arg1 N11 N11 N11 Furthermore, analysis of various N-linked glycosylation sites and cysteine mutants in gp85 revealed that glycosylation sites (N6 and N11) and cysteines (C3 and C9) were directly involved in receptor-gp85 binding and important for the entry of ALV-J into cells. 32878894 5 31 gly sites 1303:1307 arg1 glycosylation sites glycosylation sites sites Furthermore, analysis of various N-linked glycosylation sites and cysteine mutants in gp85 revealed that glycosylation sites (N6 and N11) and cysteines (C3 and C9) were directly involved in receptor-gp85 binding and important for the entry of ALV-J into cells. 32878894 5 34 gly glycosylation 1289:1301 arg2 glycosylation sites glycosylation sites sites Furthermore, analysis of various N-linked glycosylation sites and cysteine mutants in gp85 revealed that glycosylation sites (N6 and N11) and cysteines (C3 and C9) were directly involved in receptor-gp85 binding and important for the entry of ALV-J into cells. 32878894 5 34 gly glycosylation 1289:1301 arg2 N11 N11 N11 Furthermore, analysis of various N-linked glycosylation sites and cysteine mutants in gp85 revealed that glycosylation sites (N6 and N11) and cysteines (C3 and C9) were directly involved in receptor-gp85 binding and important for the entry of ALV-J into cells. 32878894 5 85 gly glycosylation 1226:1238 arg2 various N-linked glycosylation sites various N-linked glycosylation sites sites and cysteine Furthermore, analysis of various N-linked glycosylation sites and cysteine mutants in gp85 revealed that glycosylation sites (N6 and N11) and cysteines (C3 and C9) were directly involved in receptor-gp85 binding and important for the entry of ALV-J into cells. 32878894 5 87 gly cysteines 1326:1334 arg1 cysteines cysteines cysteines Furthermore, analysis of various N-linked glycosylation sites and cysteine mutants in gp85 revealed that glycosylation sites (N6 and N11) and cysteines (C3 and C9) were directly involved in receptor-gp85 binding and important for the entry of ALV-J into cells. 33738296 5 14 part_of BCP 1040:1042 arg1 The chemical composition BCP The chemical composition OGER Site BCP Q63652 position The chemical composition of BCP was determined through high performance liquid chromatography (HPLC). 33383703 2 10 gly glycoproteins 248:260 arg1 polysaccharides glycoproteins polysaccharides Fterm glycoproteins The tree's sap is a complex mixture of compounds, such as catechol lipids, polysaccharides, glycoproteins, enzymes, and water. 34753028 11 64 gly factor-κB 1621:1629 arg1 the D-Gal-induced translocation factor-κB the D-Gal-induced translocation Fterm factor-κB In addition, the D-Gal-induced translocation of nuclear factor-κB (NF-κB), as well as activation of the pro-apoptotic factors Caspase-3 and the PI3K/Akt pathways were inhibited by PG and RG. 33154460 2 49 gly acid-galactose-N-acetylgalactosamine 285:320 arg1 the threonine420 (Thr420) residue the threonine420 (Thr420) residue the threonine420 (Thr420) residue Site residue Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. 33154460 2 16 gly contains 242:249 arg1 Gc1 AND GalNAc Gc1 GalNAc PUBTATOR Gc1 10562 Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. 33154460 2 16 gly contains 242:249 arg1 Gc1 AND an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine Gc1 an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine PUBTATOR Gc1 10562 Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. 32579360 4 27 gly glycosylation 707:719 arg2 the Fc N-linked glycosylation site the Fc N-linked glycosylation site site To overcome this, we recently developed an effectorless TfRMAb-EPO fusion protein, designated TfRMAb-N292G-EPO, by eliminating the Fc N-linked glycosylation site at position 292 of the antibody heavy chain. 33559614 6 58 part_of domain 895:900 arg1 a large inserted cap domain domain a large inserted cap domain domain The enzyme possesses a typical α/β-hydrolase-fold domain with a large inserted cap domain, which together form a bowl-shaped hemispherical shape with a surface concavity surrounded by N-linked glycans. 33559614 6 64 part_of possesses 856:864 arg1 The enzyme AND a typical α/β-hydrolase-fold domain The enzyme a typical α/β-hydrolase-fold domain Fterm Site enzyme domain The enzyme possesses a typical α/β-hydrolase-fold domain with a large inserted cap domain, which together form a bowl-shaped hemispherical shape with a surface concavity surrounded by N-linked glycans. 32518939 10 73 gly O-glycosylated 1678:1691 arg1 a triple O-glycosylated EGF-LD a triple O-glycosylated EGF-LD OGER EGF P01132 Overall, these results are consistent with the presence of a triple O-glycosylated EGF-LD in mouse PAMR1. 32910214 1 68 gly multi-glycosylated 258:275 arg1 mono- and multi-glycosylated peptides mono- and multi-glycosylated peptides peptides A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. 34738170 11 11 part_of GPI-anchor 1942:1951 arg1 The GPI-anchor domain GPI The GPI-anchor domain OGER Site GPI P06744 domain • The GPI-anchor domain of Pwp1p is important for suppressing the growth defect of och1Δ cells. 33276951 4 39 gly Deglycosylation 455:469 arg1 SARS-CoV-2-NP SARS-CoV-2-NP OGER SARS P49591 Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. 34105348 7 25 gly peptides 1177:1184 arg1 33 proteins proteins peptides Fterm proteins Using the CpOGAD298N affinity column, we identified 84 highly confident O-GlcNAc modified peptides from 82 proteins in the MCF-7 cell line and 33 highly confident peptides in 33 proteins from mouse liver tissue; most of them are novel O-GlcNAc proteins and could not bind with wheat germ agglutinin (WGA). 34105348 7 78 gly proteins 1194:1201 arg1 84 highly confident O-GlcNAc modified peptides proteins 84 highly confident O-GlcNAc modified peptides Fterm proteins Using the CpOGAD298N affinity column, we identified 84 highly confident O-GlcNAc modified peptides from 82 proteins in the MCF-7 cell line and 33 highly confident peptides in 33 proteins from mouse liver tissue; most of them are novel O-GlcNAc proteins and could not bind with wheat germ agglutinin (WGA). 32482891 2 79 part_of contains 315:322 arg1 ACLP AND a collagen-binding discoidin domain ACLP domain PUBTATOR Site ACLP 165 domain ACLP contains thrombospondin repeats, a collagen-binding discoidin domain, and a catalytically inactive metallocarboxypeptidase domain. 34290711 2 1 gly fucosylation 312:323 arg1 asparagine (N)-linked glycans asparagine (N)-linked glycans Changes in terminal sialylation and fucosylation of asparagine (N)-linked glycans have been documented in COPD, but the role that glycosyltransferases may play in the regulation of N-linked glycans in COPD has not been fully elucidated. 34290711 2 6 gly sialylation 296:306 arg1 asparagine (N)-linked glycans asparagine (N)-linked glycans Changes in terminal sialylation and fucosylation of asparagine (N)-linked glycans have been documented in COPD, but the role that glycosyltransferases may play in the regulation of N-linked glycans in COPD has not been fully elucidated. 34335620 7 71 part_of GP-specific 1405:1415 arg1 1,512 GP-specific mAb variable gene sequences 1,512 GP 1,512 GP-specific mAb variable gene sequences PUBTATOR Site 1,512 GP 55819 sequences We first identified 1,512 GP-specific mAb variable gene sequences from single cells in the memory B cell compartment. 34363524 4 9 part_of GLP 525:527 arg1 known 17 germins and GLP sequences GLP known 17 germins and GLP sequences OGER Site GLP Q9H9B1 sequences In this study, 80 putative germins and GLPs have been identified in barley by using known 17 germins and GLP sequences. 32931036 8 22 part_of mGlu7 1167:1171 arg1 the binding domain mGlu7 the binding domain Cterm Site mGlu7 2917 domain In addition, we identify the binding domain of mGlu7 to Elfn1, a transsynaptic adhesion protein. 32684451 6 1 part_of SLAMF7 1192:1197 arg1 different SLAMF7 gene DNA fragments SLAMF7 different SLAMF7 gene DNA fragments PUBTATOR Site SLAMF7 Q9NQ25 fragments Based on different SLAMF7 gene DNA fragments, 2 alternative variants of this gene (V1 and V2) and complete gene expression were identified. 32684451 6 36 part_of DNA 1204:1206 arg1 different SLAMF7 gene DNA fragments DNA different SLAMF7 gene DNA fragments Cterm Site DNA fragments Based on different SLAMF7 gene DNA fragments, 2 alternative variants of this gene (V1 and V2) and complete gene expression were identified. 32264694 2 21 gly glycoprotein 140:151 arg1 a glycoprotein hormone receptor a glycoprotein hormone receptor Fterm glycoprotein The thyroid-stimulating hormone receptor (TSHR) is a glycoprotein hormone receptor which has a pivotal role in metabolic regulation and photoperiod control during reproduction in birds and mammals. 34155551 8 8 gly glycosylation 1743:1755 arg1 the spike protein the spike protein PUBTATOR spike protein 43740568 In addition, we reported on the identification of mannose-6-phosphate (M6P) glycosylation, which substantially expands the current knowledge of the spike protein's glycosylation landscape and enables future investigation into the influence of M6P glycosylation of the spike protein on its cell entry. 32910214 1 38 part_of multi-glycosylated 258:275 arg1 mono- and multi-glycosylated peptides multi mono- and multi-glycosylated peptides OGER Site multi peptides A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. 32910214 1 58 part_of MOF 176:178 arg1 A hydrophilic terpolymer MOF composite MOF A hydrophilic terpolymer MOF composite OGER Site MOF Q9H7Z6 composite A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. 33903632 5 16 gly glycosylation 792:804 arg2 N-linked glycosylation site mutagenesis N-linked glycosylation site mutagenesis site Using a structure-based engineering approach, we combined knob-into-hole Fc technology and N-linked glycosylation site mutagenesis for half-life extension, improved solubility and protease resistance. 32920057 1 21 gly glycoproteins 234:246 arg1 N-linked and O-linked glycoproteins N-linked and O-linked glycoproteins Fterm glycoproteins INTRODUCTION Galectins are proteins that bind β-galactosides such as N-acetylactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated fibrotic mechanisms. 33924503 9 10 gly glycoproteins 1349:1361 arg1 glycoproteins glycoproteins Fterm glycoproteins Changes in glycoside composition of glycoproteins on the platelets' surface impaired their functional capacity and increased their apoptosis. 33370382 8 37 part_of Env 1373:1375 arg1 other Env epitopes Env other Env epitopes PUBTATOR Site Env 100616444 epitopes These data highlight the contribution of SP sequence diversity in shaping the Env glycan content and its impact on the configuration and accessibility of V1V2 and other Env epitopes. 33335689 1 8 part_of G1 161:162 arg1 The hinge region G1 (IgG1 The hinge region OGER Site G1 (IgG1 P01857 region The hinge region of immunoglobulin G1 (IgG1) is used as a common linker for Fc-fusion therapeutic proteins. 33390949 6 45 part_of have 1098:1101 arg1 APS-Ⅲ AND different attachment sites APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33390949 6 45 part_of have 1098:1101 arg1 APS-Ⅰ AND different attachment sites APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33390949 6 45 part_of have 1098:1101 arg1 APS-Ⅰ AND different attachment sites APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 34215068 7 76 gly glycopeptides 1314:1326 arg2 glycopeptides glycopeptides glycopeptides The affinity tip demonstrated excellent performance to enrich glycopeptides and phosphopeptides with a low limit of detection up to 0.5 fmol μL-1 from tryptic digests of horseradish peroxidase and β-Casein, respectively, and was stable up to 5 rounds of enrichment. 34693225 5 2 gly glycosylated 679:690 arg1 the full-length glycosylated peptide the full-length glycosylated peptide peptide To allow for functional studies, we synthesized the full-length glycosylated peptide via solid-phase peptide synthesis, combined with diselenide-selenoester ligation-deselenization chemistry. 32146834 6 90 part_of serotonin 1012:1020 arg1 5-HT-GLU serotonin 5-HT-GLU Fterm AminoAcid serotonin GLU To demonstrate the functional effects of fecal enzymatic activity, we examined β-glucuronidase-mediated cleavage of serotonin β-d-glucuronide (5-HT-GLU) and the resultant production of free 5-HT by HPLC. 34489534 6 71 gly N-glycosylated 1092:1105 arg1 fully N-glycosylated Piezo1 protein fully N-glycosylated Piezo1 protein PUBTATOR N-glycosylated Piezo1 protein 9780 Importantly, trafficking-defective Piezo1 variants linked to generalized lymphatic dysplasia and bicuspid aortic valve display reduced fully N-glycosylated Piezo1 protein. 34026840 3 52 part_of HdhCA 505:509 arg1 The complete sequence CA II The complete sequence PUBTATOR Site CA II 760 sequence The complete sequence of HdhCA II was 1,169 bp, encoding a polypeptide of 349 amino acids with a NH2-terminal signal peptide and a CA architectural domain. 32498907 5 6 gly 330 N-glycosylation 1074:1092 arg2 330 N-glycosylation sites 330 N-glycosylation sites sites Up to 26 N-glycopeptides were identified from IgG digest, and 565 N-glycopeptides and 330 N-glycosylation sites, mapped to 159 glycoproteins, were identified from 2 μL human serum digest, indicating the great ability for enriching N-glycopeptides from complex biological samples. 32498907 5 27 gly glycoproteins 1115:1127 arg1 159 glycoproteins 159 glycoproteins Fterm glycoproteins Up to 26 N-glycopeptides were identified from IgG digest, and 565 N-glycopeptides and 330 N-glycosylation sites, mapped to 159 glycoproteins, were identified from 2 μL human serum digest, indicating the great ability for enriching N-glycopeptides from complex biological samples. 32498907 5 35 gly 26 N-glycopeptides 994:1011 arg2 26 N-glycopeptides 26 N-glycopeptides 26 N-glycopeptides Up to 26 N-glycopeptides were identified from IgG digest, and 565 N-glycopeptides and 330 N-glycosylation sites, mapped to 159 glycoproteins, were identified from 2 μL human serum digest, indicating the great ability for enriching N-glycopeptides from complex biological samples. 32498907 5 51 gly N-glycopeptides 1219:1233 arg2 N-glycopeptides N-glycopeptides N-glycopeptides Up to 26 N-glycopeptides were identified from IgG digest, and 565 N-glycopeptides and 330 N-glycosylation sites, mapped to 159 glycoproteins, were identified from 2 μL human serum digest, indicating the great ability for enriching N-glycopeptides from complex biological samples. 32498907 5 63 gly 565 N-glycopeptides 1050:1068 arg2 565 N-glycopeptides 565 N-glycopeptides 565 N-glycopeptides Up to 26 N-glycopeptides were identified from IgG digest, and 565 N-glycopeptides and 330 N-glycosylation sites, mapped to 159 glycoproteins, were identified from 2 μL human serum digest, indicating the great ability for enriching N-glycopeptides from complex biological samples. 33997889 7 39 part_of ComP 1069:1072 arg1 the minimal ComP sequon ComP the minimal ComP sequon OGER Site ComP P49747 sequon Here we sought to identify the minimal ComP sequon sufficient for PglS glycosylation. 32222585 4 57 gly glycosylation 764:776 arg2 their glycosylation sites their glycosylation sites sites The last few years have thus seen the emergence of many studies investigating the complex influence of FcγRIIIa N-glycans on the interaction with IgG1 through their glycosylation sites or their glycoprofiles. 34734627 2 3 gly occupancy 381:389 arg2 specific glycosylation sites specific glycosylation sites sites Previously, it has been shown that there is a temporal relationship between protein folding and glycosylation, which influences the occupancy of specific glycosylation sites. 34734627 2 17 gly glycosylation 403:415 arg2 specific glycosylation sites specific glycosylation sites sites Previously, it has been shown that there is a temporal relationship between protein folding and glycosylation, which influences the occupancy of specific glycosylation sites. 32157140 11 62 gly N-glycosylation 1378:1392 arg1 human GPR176 GPR176 sites PUBTATOR GPR176 11245 sites Importantly, missense variations in the conserved N-glycosylation sites of human GPR176 (rs1473415441; rs761894953) affected N-glycosylation and thereby attenuated protein expression and cAMP-repressive activity in the cells. 33755116 2 33 gly glycosylation 192:204 arg1 pathogenic proteins pathogenic proteins Fterm proteins For example, glycosylation of pathogenic proteins is a common immune evasive mechanism, hampering the development of successful vaccines. 32441096 2 64 gly fucosylated 320:330 arg1 fucosylated N-glycans fucosylated N-glycans Because of the many possible anomeric linkages associated with fucosylated N-glycans, determination of specific anomeric linkages and the site of fucosylation (i.e., core vs outer arm) can be difficult to elucidate. 32598381 6 103 part_of NA 965:966 arg1 The 245 NA Gly+ 245 NA The 245 NA Gly+ Cterm Site 245 NA Gly+ The 245 NA Gly+ blocked human and rabbit monoclonal antibodies that target the enzymatic site from binding to their epitope. 34646249 7 29 gly glycosylation 936:948 arg2 amino acid position 200 position 200 position 200 Moreover, we found that residue 199 contributed to inducing broad protective antibodies without the influence of the N-linked glycosylation at amino acid position 200 in NAs. 34636569 7 72 gly glycopeptides 1460:1472 arg2 glycopeptides glycopeptides M6P and fucosylation co-modifications glycopeptides This is hitherto the first report of M6P and fucosylation co-modifications of glycopeptides, in which the mechanism and function still needs further exploration. 34322281 1 81 gly N-acetylgalactosaminyl 103:124 arg1 GalNAc-T N-acetylgalactosaminyl GalNAc-T Cterm N-acetylgalactosaminyl The polypeptide N-acetylgalactosaminyl transferase (GalNAc-T) enzyme family initiates O-linked mucin-type glycosylation. 34284575 4 8 part_of sites 511:515 arg1 this protein protein sites Fterm Site protein sites Unlike the 22 confirmed N-glycosylation sites on SARS-CoV-2 S, only a few O-glycosylation sites on this protein have been reported. 32987930 4 11 gly N-glycosylation 628:642 arg2 the N-glycosylation consensus motifs the N-glycosylation consensus motifs motifs In this study, we mutated the N-glycosylation consensus motifs of the E1 and E2 glycoproteins of a SAV3 infectious clone using site-directed mutagenesis. 32987930 4 36 gly glycoproteins 678:690 arg1 the E1 and E2 glycoproteins the E1 and E2 glycoproteins PUBTATOR E1 and E2 glycoproteins 6080 In this study, we mutated the N-glycosylation consensus motifs of the E1 and E2 glycoproteins of a SAV3 infectious clone using site-directed mutagenesis. 34400147 5 46 gly glycosylation 885:897 arg2 glycosylation sites glycosylation sites sites We identified oligomeric states with glycosylation sites that are inaccessible to enzymatic removal. 33004979 6 2 part_of bile 907:910 arg1 fecal bile acid composition bile acid fecal bile acid composition PUBTATOR Site bile acid 230101 position Mdr2-/- mice demonstrate alterations in fecal bile acid composition and enhanced colitis susceptibility with increased colonic adhesion molecule expression, particularly mucosal addressin-cell adhesion molecule 1 (MAdCAM-1). 33004979 6 50 part_of acid 912:915 arg1 fecal bile acid composition bile acid fecal bile acid composition PUBTATOR Site bile acid 230101 position Mdr2-/- mice demonstrate alterations in fecal bile acid composition and enhanced colitis susceptibility with increased colonic adhesion molecule expression, particularly mucosal addressin-cell adhesion molecule 1 (MAdCAM-1). 32099050 0 77 gly cross-reactivity 10:25 arg1 shared glycanic epitopes shared glycanic epitopes epitopes Antigenic cross-reactivity between Schistosoma mansoni and allergenic invertebrates putatively due to shared glycanic epitopes. 34478712 8 44 gly glycopeptides 1363:1375 arg2 Pronase-digested glycopeptides Pronase-digested glycopeptides glycopeptides Methylation linkage analysis of Pronase-digested glycopeptides isolated from BSF wild-type and TbGT10 null parasites showed a reduction in 6-O-substituted- and 3,6-di-O-substituted-Gal residues. 34677046 12 69 gly glycosites 1728:1737 arg2 novel glycosites novel glycosites glycosites This study serves as a model for the use of OST-negative cells and proteomics for highlighting novel glycosites and determining occupancy in a range of organisms. 32482891 2 79 gly contains 315:322 arg1 ACLP AND thrombospondin repeats ACLP a collagen-binding discoidin domain thrombospondin repeats PUBTATOR Site ACLP 165 domain ACLP contains thrombospondin repeats, a collagen-binding discoidin domain, and a catalytically inactive metallocarboxypeptidase domain. 33906120 11 17 gly glycosylated 1938:1949 arg1 N-linked glycosylated proteins N-linked glycosylated proteins Fterm proteins Our results indicated that N-linked glycosylated proteins could play important roles in the early seedling growth of wheat. 33733058 6 25 gly ACE2 1299:1302 arg1 the sialylation status ACE2 the sialylation status OGER ACE2 Q9BYF1 We hypothesize that modulating the activity of one of them, the lysosomal sialidase NEU1, could impinge on both the sialylation status of ACE2 and other host receptors as well as the extent of lysosomal exocytosis. 33733058 6 68 gly sialylation 1277:1287 arg1 ACE2 ACE2 OGER ACE2 Q9BYF1 We hypothesize that modulating the activity of one of them, the lysosomal sialidase NEU1, could impinge on both the sialylation status of ACE2 and other host receptors as well as the extent of lysosomal exocytosis. 33547363 7 1 part_of had 1259:1261 arg1 The identified peptides AND 81% NXT-type motifs The identified peptides 81% NXT-type motifs motifs The identified peptides had 81% NXT-type motifs, with X being any amino acids except proline. 32002580 10 34 gly glycopeptide 1600:1611 arg2 N-linked glycopeptide enrichment N-linked glycopeptide enrichment glycopeptide Thus, the presented research work can be an efficient separation platform for N-linked glycopeptide enrichment from complex samples, which can be extended to cost-effective routine analysis. 32518939 7 40 gly glycosylated 1075:1086 arg1 this domain to be glycosylated this domain to be glycosylated domain To test the ability of this domain to be glycosylated, a correctly folded EGF-LD was produced in Escherichia coli periplasm, purified and subjected to in vitro incubations with the recombinant O-glycosyltransferases POGLUT1, POFUT1 and EOGT, adding O-Glc1, O-Fuc and O-GlcNAc, respectively. 32443590 6 68 gly glycoprotein 1298:1309 arg1 the model glycoprotein RNase B the model glycoprotein RNase B Fterm glycoprotein EndoSS only hydrolyzed a part of the model glycoprotein RNase B with high-mannose N-linked glycoforms into a low molecular weight form, and the solo activity of GH92 could not show any changes comparing with the blank control in SDS-PAGE gel. 34182612 0 61 gly glycosylation 30:42 arg2 Predicted N-terminal N-linked glycosylation sites Predicted N-terminal N-linked glycosylation sites sites Predicted N-terminal N-linked glycosylation sites may underlie membrane protein expression patterns in Saccharomyces cerevisiae. 33806920 7 31 gly hypoglycosylated 1090:1105 arg1 hypoglycosylated COX-2 hypoglycosylated COX-2 PUBTATOR COX-2 5743 Finally, hypoglycosylated COX-2 showed a more rapid rate of degradation compared to COX-2 induced by IL-1α and an alteration in the localization with an accumulation mainly detected in the nuclear membrane. 32633815 11 25 part_of serine 1482:1487 arg1 pangolin pangolin serine Fterm AminoAcid pangolin serine Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O-linked glycans were seen in RaTG13, suggesting that a detailed study needed in pangolin (Manis javanica) and bat (Rhinolophus affinis) related CoV. 32633815 11 36 part_of serine 1428:1433 arg1 pangolin pangolin serine Fterm AminoAcid pangolin serine Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O-linked glycans were seen in RaTG13, suggesting that a detailed study needed in pangolin (Manis javanica) and bat (Rhinolophus affinis) related CoV. 32633815 11 40 part_of threonine 1462:1470 arg1 pangolin pangolin threonine Fterm AminoAcid pangolin threonine Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O-linked glycans were seen in RaTG13, suggesting that a detailed study needed in pangolin (Manis javanica) and bat (Rhinolophus affinis) related CoV. 32188979 5 42 gly glycosylation 915:927 arg1 Hyl Hyl OGER Hyl P42679 In this study, we first adapted the force field parameters for O-linkages between Hyl and carbohydrates and then investigated the influence of Hyl-O-glycosylation on the structure of type I collagen molecule, by performing comprehensive molecular dynamic simulations in explicit solvent of collagen molecule segment with and without the glycosylation of Hyl. 32393178 0 34 gly glycosylation 139:151 arg1 Hemagglutinin Hemagglutinin Fterm Hemagglutinin Multi-task learning sparse group lasso: a method for quantifying antigenicity of influenza A(H1N1) virus using mutations and variations in glycosylation of Hemagglutinin. 32987930 4 19 part_of glycoproteins 678:690 arg1 the N-glycosylation consensus motifs E1 and E2 glycoproteins the N-glycosylation consensus motifs PUBTATOR Site E1 and E2 glycoproteins 6080 motifs In this study, we mutated the N-glycosylation consensus motifs of the E1 and E2 glycoproteins of a SAV3 infectious clone using site-directed mutagenesis. 33309857 0 73 gly glycosylation 116:128 arg1 CDK11p110 CDK11p110 PUBTATOR CDK11p110 984 Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. 34451558 0 18 gly Remodeling 34:43 arg1 Common Bean Common Bean Remodeling OGER Common Bean Q3B7T3 Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean. 34455356 10 57 part_of NS1 2085:2087 arg1 N-linked glycosylation sites NS1 N-linked glycosylation sites OGER Site NS1 Q9Y6Y0 sites Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N-linked glycosylation sites N N-linked glycosylation sites Cterm Site N sites Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 57 part_of NS1 2085:2087 arg1 N130 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 57 part_of NS1 2085:2087 arg1 N175 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 57 part_of NS1 2085:2087 arg1 N175 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N130 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N175 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N175 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34677377 5 29 gly κ-casein 1018:1025 arg1 O-linked glycans -casein O-linked glycans PUBTATOR -casein 281728 Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-d-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. 34677377 5 29 gly κ-casein 1018:1025 arg1 certain oligosaccharide isomers -casein certain oligosaccharide isomers PUBTATOR -casein 281728 Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-d-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. 33182731 4 13 gly glycosites 956:965 arg2 many glycosites many glycosites glycosites Interestingly, the glycosites identified by SPEG and HILIC overlapped considerably whereas NGAG identified many glycosites not observed in the other two methods. 33182731 4 39 gly glycosites 863:872 arg2 the glycosites the glycosites glycosites Interestingly, the glycosites identified by SPEG and HILIC overlapped considerably whereas NGAG identified many glycosites not observed in the other two methods. 33065162 2 6 gly receptor 280:287 arg1 O-GlcNAcylation RBP4 receptor O-GlcNAcylation OGER RBP4 receptor Q00724 Disruption of the retinol cascade and O-GlcNAcylation of the RBP4 receptor (STRA6) are found in diabetic kidneys. 32815135 2 0 gly glycosylated 341:352 arg1 OmpA-like proteins OmpA-like proteins Fterm proteins Our previous studies have shown that OmpA-like proteins are glycosylated by O-linked N-acetylglucosamine (O-GlcNAc) and are strongly reactive to wheat germ agglutinin (WGA) lectin, which shows sugar specificity to GlcNAc. 31452158 5 22 gly glycoprotein 719:730 arg1 recombinant, functional Cryptosporidium glycoprotein antigens recombinant, functional Cryptosporidium glycoprotein antigens Fterm glycoprotein Here, we describe a method of producing recombinant, functional Cryptosporidium glycoprotein antigens in Toxoplasma gondii. 32337418 8 10 gly N-glycoforms 1621:1632 arg1 subclass-specific IgG Fc N-glycoforms subclass-specific IgG Fc N-glycoforms PUBTATOR IgG 16059 This report demonstrates a method with great reliability and practicality that has promising potential for the relative quantitation of subclass-specific IgG Fc N-glycoforms in multiple mouse models. 34738170 1 21 gly chain 192:196 arg1 N-linked oligosaccharides chain N-linked oligosaccharides OGER chain P06744 The glycoproteins of yeast contain a large outer chain on N-linked oligosaccharides; therefore, yeast is not suitable for producing therapeutic glycoproteins for human use. 34738170 1 57 gly glycoproteins 147:159 arg1 The glycoproteins The glycoproteins Fterm glycoproteins The glycoproteins of yeast contain a large outer chain on N-linked oligosaccharides; therefore, yeast is not suitable for producing therapeutic glycoproteins for human use. 34738170 1 81 gly glycoproteins 287:299 arg1 therapeutic glycoproteins therapeutic glycoproteins Fterm glycoproteins The glycoproteins of yeast contain a large outer chain on N-linked oligosaccharides; therefore, yeast is not suitable for producing therapeutic glycoproteins for human use. 33346724 9 19 gly glycoprotein 1237:1248 arg1 N-linked glycoprotein glycans N-linked glycoprotein glycans Fucosylation and sialylation of N-linked glycoprotein glycans are affected by LoDIR and a subset of N-glycans are involved in the organismal radio-response. 33346724 9 23 gly Fucosylation 1196:1207 arg1 N-linked glycoprotein glycans N-linked glycoprotein glycans Fucosylation and sialylation of N-linked glycoprotein glycans are affected by LoDIR and a subset of N-glycans are involved in the organismal radio-response. 33346724 9 58 gly sialylation 1213:1223 arg1 N-linked glycoprotein glycans N-linked glycoprotein glycans Fucosylation and sialylation of N-linked glycoprotein glycans are affected by LoDIR and a subset of N-glycans are involved in the organismal radio-response. 33924774 5 76 gly glycosylation 629:641 arg1 the ANTXR1 vWA domain domain domain Here we show that N-linked glycosylation in the ANTXR1 vWA domain is necessary for SVV attachment and entry. 32709339 5 43 gly fragment 776:783 arg1 sugar fragment ions sugar fragment ions The saccharide moieties produced sugar fragment ions from m/z (monosaccharide) to m/z (polysaccharides). 32046000 3 19 part_of BclA3 601:605 arg1 BclA3 synthetic peptides BclA3 BclA3 synthetic peptides Cterm Site BclA3 peptides In vitro assays using the recombinant enzyme and BclA3 synthetic peptides demonstrated that SgtA GT was responsible for the addition of β-O-linked GlcNAc to threonine residues of each synthetic peptide. 32730198 7 41 part_of SURF-ANA1T 999:1008 arg1 the 16S rRNA gene sequence SURF-ANA1T the 16S rRNA gene sequence Cterm Site SURF-ANA1T sequence Phylogenetic analysis based on the 16S rRNA gene sequence of strain SURF-ANA1T indicated a closest relationship with the recently characterized Rectinema cohabitans (99%). 32958677 4 17 gly N-glycosylated 860:873 arg1 two naturally occurring misfolded N-glycosylated variants two naturally occurring misfolded N-glycosylated variants Fterm variants To interrogate this functional dichotomy, the intracellular fates of two naturally occurring misfolded N-glycosylated variants of human alpha1-antitrypsin (AAT), Null Hong Kong (NHK), and Z (ATZ), in Man1b1 knockout HEK293T cells were monitored in response to mutated or truncated forms of transfected Man1b1. 34188486 6 25 gly glycosylation 960:972 arg2 potential N-linked glycosylation sites potential N-linked glycosylation sites sites In CellBD, the cleavages were shown to occur close to potential N-linked glycosylation sites, suggesting that these sites might serve as 'attributive tags' for differentiating rCenA from endogenous proteins and the points of initiation of SC. 33168911 13 98 gly O-GlcNAcylation 1948:1962 arg1 His-tagged human eNOS His-tagged human eNOS PUBTATOR eNOS 4846 Conversely, in His-tagged human eNOS carrying a mutation at Thr866, O-GlcNAcylation was unaffected by glucose deprivation. 33036363 6 14 part_of ARF 1201:1203 arg1 the overall composition ARF the overall composition OGER Site ARF Q8N726 position Overall, transition of steers from a baseline hay diet to alfalfa pastures was associated with drastic changes in the composition of the fungal community, but the overall composition of ARF did not differ (p > 0.05) among bloated and non-bloated steers. 32510947 6 19 gly proteins 1432:1439 arg1 O-GlcNAcylated residues proteins O-GlcNAcylated residues Fterm proteins We expect this strategy will complement existing MS/MS methods and be broadly useful for mapping O-GlcNAcylated residues of both proteins and proteomes. 33981677 10 64 gly N-glycopeptides 2015:2029 arg2 2,197 N-glycopeptides 2,197 N-glycopeptides N-glycopeptides The application of a TMF-immobilized enzyme in large-scale profiling of protein N-glycosylation in urine samples led to the successful identification of 2,197 N-glycopeptides and further demonstrated the potential of this strategy for fast and high-throughput analysis of N-glycoproteome in clinical samples. 34555499 5 92 gly glycoprotein 1043:1054 arg1 the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b PUBTATOR P-selectin glycoprotein ligand-1 Q14242 To study the specificity of the potential ovarian sulfotransferases involved, Gal3ST2 (Gal-3S)-, Gal3ST4 (Gal-3S)-, and CHST1 (Gal-6S)-encoding expression plasmids were transfected individually into CHO cells also expressing the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b (PSGL-1/mIg G2b) fusion protein and the human core 2 transferase (GCNT1). 34051448 2 10 gly glycoproteins 347:359 arg1 N- linked glycoproteins N- linked glycoproteins Fterm glycoproteins This enzyme functions in cytosolic deglycosylation of N- linked glycoproteins. 34051448 2 18 gly deglycosylation 318:332 arg1 N- linked glycoproteins N- linked glycoproteins Fterm glycoproteins This enzyme functions in cytosolic deglycosylation of N- linked glycoproteins. 33073165 5 26 gly Glycosylation 885:897 arg1 Hyl Hyl OGER Hyl P42679 Glycosylation of Hyl was found to strongly down-regulate the binding of CD44 and the α3β1 integrin to collagen, while the impact on α2β1 integrin binding was more modest. 33624753 8 14 part_of Ras-GAP 1550:1556 arg1 the intracellular Ras-GAP domain GAP the intracellular Ras-GAP domain OGER Site GAP P20936 domain Three-dimensional molecular models revealed a highly conserved structural architecture of choanoflagellate Plexin-1 as compared to metazoan plexins, including similar predicted conformational changes in a segment that is involved in the activation of the intracellular Ras-GAP domain. 34322281 9 70 part_of peptides 1193:1200 arg1 serine proline, serine, threonine, and alanine proline, serine, threonine, and alanine peptides with proline, serine, threonine, and alanine at the -1 position of the peptide preferentially exhibited cognate sequon-like conformations. 34322281 9 70 part_of peptides 1193:1200 arg1 proline proline, serine, threonine, and alanine proline, serine, threonine, and alanine peptides with proline, serine, threonine, and alanine at the -1 position of the peptide preferentially exhibited cognate sequon-like conformations. 34322281 9 70 part_of peptides 1193:1200 arg1 threonine proline, serine, threonine, and alanine proline, serine, threonine, and alanine peptides with proline, serine, threonine, and alanine at the -1 position of the peptide preferentially exhibited cognate sequon-like conformations. 34322281 9 70 part_of peptides 1193:1200 arg1 proline proline, serine, threonine, and alanine proline, serine, threonine, and alanine peptides with proline, serine, threonine, and alanine at the -1 position of the peptide preferentially exhibited cognate sequon-like conformations. 34322281 9 70 part_of peptides 1193:1200 arg1 threonine proline, serine, threonine, and alanine proline, serine, threonine, and alanine peptides with proline, serine, threonine, and alanine at the -1 position of the peptide preferentially exhibited cognate sequon-like conformations. 34322281 9 70 part_of peptides 1193:1200 arg1 threonine proline, serine, threonine, and alanine proline, serine, threonine, and alanine peptides with proline, serine, threonine, and alanine at the -1 position of the peptide preferentially exhibited cognate sequon-like conformations. 32002580 1 67 gly glycopeptides 128:140 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides Highly specific enrichment of N-linked glycopeptides from complex biological samples is crucial prior to mass spectrometric analysis. 34459483 3 50 gly sialylated 672:681 arg1 sialylated core 2 O-glycan structures sialylated core 2 O-glycan structures We previously determined that the ability of S. gordonii to bind sialyl T-antigen (sTa) increased pathogenicity, relative to recognition of sialylated core 2 O-glycan structures, in an animal model of IE. 32157440 1 6 gly glycosylation 184:196 arg1 proteins proteins Fterm proteins Post-translational glycosylation of proteins with O-linked β-N-acetylglucosamine (O-GlcNAcylation) and changes of galectin expression profiles are essential in many cellular stress responses. 31860302 7 34 gly N-glycopeptides 1318:1332 arg2 1644 unique intact N-glycopeptides 1644 unique intact N-glycopeptides N-glycopeptides Finally, a large and comprehensive human plasma N-glycoproteomic database was established, containing 786 proteins, 369 N-glycoproteins, 862 glycosites, 171 glycan compositions, and 1644 unique intact N-glycopeptides. 31860302 7 46 gly glycosites 1258:1267 arg2 862 glycosites 862 glycosites glycosites Finally, a large and comprehensive human plasma N-glycoproteomic database was established, containing 786 proteins, 369 N-glycoproteins, 862 glycosites, 171 glycan compositions, and 1644 unique intact N-glycopeptides. 31860302 7 60 gly N-glycoproteins 1237:1251 arg1 369 N-glycoproteins 369 N-glycoproteins Fterm N-glycoproteins Finally, a large and comprehensive human plasma N-glycoproteomic database was established, containing 786 proteins, 369 N-glycoproteins, 862 glycosites, 171 glycan compositions, and 1644 unique intact N-glycopeptides. 32331895 7 90 gly N-glycosylation 1101:1115 arg2 All 5 putative N-glycosylation sites All 5 putative N-glycosylation sites sites All 5 putative N-glycosylation sites in this loop were altered by site-directed mutagenesis, and the mutant construct was transfected into CHO cells for transport activity assay. 34459483 7 60 gly sTa 1330:1332 arg1 Proteoglycan 4 sTa Proteoglycan 4 OGER sTa P50402 Proteoglycan 4 (PRG4), also known as lubricin, was a major carrier of sTa in the infected vegetations. 33603735 0 33 gly Modification 29:40 arg1 Mitochondrial Antiviral Signaling Protein AND O-Linked N-Acetylglucosamine Modification Mitochondrial Antiviral Signaling Protein O-Linked N-Acetylglucosamine Modification OGER Mitochondrial Antiviral Signaling Protein Regulates Q7Z434 O-Linked N-Acetylglucosamine Modification of Mitochondrial Antiviral Signaling Protein Regulates Antiviral Signaling by Modulating Its Activity. 33603735 0 35 gly Protein 79:85 arg1 O-Linked N-Acetylglucosamine Modification Mitochondrial Antiviral Signaling Protein Regulates O-Linked N-Acetylglucosamine Modification OGER Mitochondrial Antiviral Signaling Protein Regulates Q7Z434 O-Linked N-Acetylglucosamine Modification of Mitochondrial Antiviral Signaling Protein Regulates Antiviral Signaling by Modulating Its Activity. 33301684 3 23 gly glycoprotein 476:487 arg1 glycoprotein isolation glycoprotein isolation Fterm glycoprotein The typical glycomics analysis approaches used to quantify uromodulin glycosylation involve time-consuming and tedious glycoprotein isolation and labeling steps, which limit their utility in clinical glycomics assays, where sample throughput is important. 32510947 1 22 part_of sites 309:313 arg1 proteins proteins sites Fterm Site proteins sites Despite its central importance as a regulator of cellular physiology, identification and precise mapping of O-linked N-acetylglucosamine (O-GlcNAc) post-translational modification (PTM) sites in proteins by mass spectrometry (MS) remains a considerable technical challenge. 34418081 7 9 gly glycosylation 1025:1037 arg1 AZU1 AZU1 PUBTATOR AZU1 566 Collectively, we found that N-linked glycosylation of AZU1 directs its loading into SEVs, thereby enabling AZU1-positive SEVs to function as potent permeabilizers of endothelial cells and leading to enhanced transendothelial migration of RCC cells. 34327998 2 35 gly glycopeptide 350:361 arg2 a different glycopeptide a different glycopeptide glycopeptide The representation takes the form of a simple mass spectrum for a given peptide sequence, with each peak corresponding to a different glycopeptide. 33242079 11 37 gly sialylated 1797:1806 arg1 sialylated O-linked glycoproteins sialylated O-linked glycoproteins Fterm glycoproteins In the future, where and how sialylated O-linked glycoproteins function in the brain tissue remains to be clarified. 33242079 11 86 gly glycoproteins 1817:1829 arg1 sialylated O-linked glycoproteins sialylated O-linked glycoproteins Fterm glycoproteins In the future, where and how sialylated O-linked glycoproteins function in the brain tissue remains to be clarified. 34644163 6 93 gly glycosylated 881:892 arg1 The glycosylated protein The glycosylated protein Fterm protein The glycosylated protein was sensitive to peptide-N-glycosidase F, which can hydrolyze N-linked oligosaccharides. 34284575 9 25 gly glycopeptides 1285:1297 arg2 O-linked glycopeptides O-linked glycopeptides glycopeptides Our study demonstrates that the biomimetic polymer can offer specific selectivity for O-linked glycopeptides and pave the way for O-glycosylation research in biological fields. 32193337 5 19 gly modified 729:736 arg1 AHCY AND an O-linked β-N-acetylglucosamine sugar AHCY an O-linked β-N-acetylglucosamine sugar PUBTATOR AHCY 269378 AHCY is posttranslationally modified with an O-linked β-N-acetylglucosamine sugar (O-GlcNAcylation), which is rapidly removed upon differentiation. 33624753 7 63 part_of contain 1185:1191 arg1 Sema-FN1 AND a short homologous motif Sema-FN1 a short homologous motif PUBTATOR Site Both Sema 34192 motif Both Sema-FN1 and Plexin-1 also contain a short homologous motif near the C-terminus, likely associated with a shared function. 34459483 0 35 gly glycoprotein 49:60 arg1 a highly sialylated glycoprotein glycoprotein Proteoglycan 4 Fterm glycoprotein Proteoglycan 4 (lubricin) is a highly sialylated glycoprotein associated with cardiac valve damage in animal models of infective endocarditis. 34459483 0 77 gly sialylated 38:47 arg1 a highly sialylated glycoprotein glycoprotein Proteoglycan 4 Fterm glycoprotein Proteoglycan 4 (lubricin) is a highly sialylated glycoprotein associated with cardiac valve damage in animal models of infective endocarditis. 32892942 4 34 gly N-glycopeptides 606:620 arg2 N-glycopeptides enrichment N-glycopeptides enrichment N-glycopeptides The abundant hydroxyl groups on the surface make the material highly good hydrophilic and thus it was utilized for N-glycopeptides enrichment. 34023382 7 65 gly glycosylation 1028:1040 arg2 the glycosylation sequon the glycosylation sequon sequon We found that the peptide sequence close to the glycosylation sequon affected peptide affinity and turnover rate. 32598381 11 46 part_of NA 1796:1797 arg1 the 245 NA Gly+ 245 NA the 245 NA Gly+ Cterm Site 245 NA Gly+ These results suggest that while the 245 NA Gly+ decreases virus replication in hNECs and decreases enzymatic activity, the 245 NA glycan blocks the binding of monoclonal and human serum NA specific antibodies that would otherwise inhibit enzymatic activity and virus replication. 34677046 10 68 gly glycosites 1404:1413 arg2 142 glycosites 142 glycosites glycosites We identified 142 glycosites, of which 32 were novel, and 83% of sites predicted by proteomics were validated. 33023909 5 68 gly STIM1 878:882 arg1 the possible O-GlcNAcylation sites STIM1 the possible O-GlcNAcylation sites PUBTATOR STIM1 6786 Using these cells, we examined the possible O-GlcNAcylation sites of STIM1 to determine whether the sites were O-GlcNAcylated. 32157140 11 11 part_of GPR176 1409:1414 arg1 the conserved N-glycosylation sites GPR176 the conserved N-glycosylation sites PUBTATOR Site GPR176 11245 sites Importantly, missense variations in the conserved N-glycosylation sites of human GPR176 (rs1473415441; rs761894953) affected N-glycosylation and thereby attenuated protein expression and cAMP-repressive activity in the cells. 34260942 0 34 gly Psme3 33:37 arg1 Site-specific O-GlcNAcylation Psme3 Site-specific O-GlcNAcylation PUBTATOR Psme3 19192 Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis. 34155551 4 20 part_of glycoprotein 900:911 arg1 neutral and sialyl glycopeptides S glycoprotein neutral and sialyl glycopeptides PUBTATOR Site S glycoprotein 43740568 glycopeptides We explore the novel use of dual-functionalized titanium (IV)-immobilized metal affinity chromatography (Ti-IMAC) material for simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293 cells. 34227365 17 83 gly N-glycopeptides 3047:3061 arg2 urinary N-glycopeptides urinary N-glycopeptides N-glycopeptides The abundance of urinary N-glycopeptides spanned across approximately five orders of magnitude. 33454535 0 25 gly glycosylation 103:115 arg2 N-linked glycosylation sites N-linked glycosylation sites sites Incorporating a transfer learning technique with amino acid embeddings to efficiently predict N-linked glycosylation sites in ion channels. 33454535 3 83 gly glycosylation 451:463 arg2 N-linked glycosylation sites N-linked glycosylation sites sites This study used a computational method to predict N-linked glycosylation sites, the most common type, in ion channel proteins. 33906120 6 40 gly glycoproteins 862:874 arg1 65% glycoproteins 65% glycoproteins Fterm glycoproteins In terms of molecular function, 65% glycoproteins belonged to various enzymes with catalytic activity such as kinase, carboxypeptidase, peroxidase and phosphatase, and, particularly, 25% of glycoproteins were related to binding functions. 33906120 6 107 gly glycoproteins 1016:1028 arg1 glycoproteins glycoproteins Fterm glycoproteins In terms of molecular function, 65% glycoproteins belonged to various enzymes with catalytic activity such as kinase, carboxypeptidase, peroxidase and phosphatase, and, particularly, 25% of glycoproteins were related to binding functions. 33151735 1 3 gly virus 155:159 arg1 cysteine cysteine cysteine No abstract Keywords: canine distemper virus; H gene; phylogenetic analysis; amino-acid substitution; N-linked glycolysation; cysteine. 33788965 4 56 gly N-glycosylated 577:590 arg2 single Asn residues CALHM1 and 3 Asn residues PUBTATOR CALHM1 and 3 255022 Asn residues Biochemical analyses of the two subunits following site-directed mutagenesis and pharmacological treatments established that both CALHM1 and 3 were N-glycosylated at single Asn residues in their second extracellular loops. 33788965 4 56 gly N-glycosylated 577:590 arg1 CALHM1 CALHM1 and 3 PUBTATOR CALHM1 and 3 255022 Biochemical analyses of the two subunits following site-directed mutagenesis and pharmacological treatments established that both CALHM1 and 3 were N-glycosylated at single Asn residues in their second extracellular loops. 33788965 4 56 gly N-glycosylated 577:590 arg1 CALHM1 CALHM1 and 3 Asn residues PUBTATOR CALHM1 and 3 255022 Asn residues Biochemical analyses of the two subunits following site-directed mutagenesis and pharmacological treatments established that both CALHM1 and 3 were N-glycosylated at single Asn residues in their second extracellular loops. 33155130 3 12 gly proteins 600:607 arg1 carbohydrates proteins carbohydrates Fterm proteins Secreted biopolymers present polydisperse and high molecular weight distributions, with significant amounts of carbohydrates and proteins. 32393178 2 92 part_of glycoproteins 459:471 arg1 protein sequence glycoproteins protein sequence Fterm Site glycoproteins sequence Antigenic property of influenza viruses are determined by both protein sequence and N-linked glycosylation of influenza glycoproteins, especially hemagglutinin (HA). 34452648 9 21 gly glycosylation 1134:1146 arg1 integrin α5 integrin α5 PUBTATOR integrin α5 3678 C1GALT1 was able to modify O-linked glycosylation on integrin α5 and thereby modulate the activation of the PI3K/AKT pathway. 31860302 1 78 gly N-glycoproteins 107:121 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins N-glycoproteins are involved in various biological processes. 33888690 7 19 gly glycoprotein 1099:1110 arg1 cell-free N-linked and O-linked glycoprotein synthesis cell-free N-linked and O-linked glycoprotein synthesis Fterm glycoprotein Finally, we apply our methods to enrich membrane-bound oligosaccharyltransferases and lipid-linked oligosaccharides for improving cell-free N-linked and O-linked glycoprotein synthesis. 32542517 12 51 gly Neu5Gc-sialylated 1640:1656 arg1 Neu5Gc-sialylated N-glycans Neu5Gc-sialylated N-glycans Porcine pericardium carried the highest level of Neu5Gc-sialylated O-glycans, and bovine pericardium the highest level of Neu5Gc-sialylated N-glycans. 32542517 12 61 gly Neu5Gc-sialylated 1567:1583 arg1 Neu5Gc-sialylated O-glycans Neu5Gc-sialylated O-glycans Porcine pericardium carried the highest level of Neu5Gc-sialylated O-glycans, and bovine pericardium the highest level of Neu5Gc-sialylated N-glycans. 32931036 5 8 gly N-glycosylated 769:782 arg2 four asparagine residues mGlu7 asparagine residues Cterm mGlu7 2917 asparagine residues In this study, we find that mGlu7 is N-glycosylated at four asparagine residues in heterologous cells and rat cultured neurons. 34348917 9 45 gly modified 1332:1339 arg1 RUNX1 AND O-GlcNAc RUNX1 O-GlcNAc PUBTATOR RUNX1 50662 RUNX1 can be modified by O-GlcNAc, and that the modification is enhanced in a high glucose environment. 32222585 3 23 gly afucosylated 580:591 arg1 afucosylated IgG1 afucosylated IgG1 OGER IgG1 P01857 Pioneer studies on FcγR N-glycans have unveiled an additional complexity in that the N-glycan linked on the Asn-162 of FcγRIIIa was shown to be directly involved in the strong affinity for afucosylated IgG1. 32222585 3 60 gly linked 485:490 arg2 the N-glycan AND the Asn-162 the Asn-162 the N-glycan Asn-162 Pioneer studies on FcγR N-glycans have unveiled an additional complexity in that the N-glycan linked on the Asn-162 of FcγRIIIa was shown to be directly involved in the strong affinity for afucosylated IgG1. 32522876 6 20 gly O-glycosylated 1045:1058 arg1 O-glycosylated proteins O-glycosylated proteins Fterm proteins Whole-genome analysis showed that O-glycosylated proteins and TRIM28 were specifically bound to promoters of active retrotransposons and to imprinting control regions, the two major regulatory sequences controlled by DNA methylation. 33877808 2 16 gly glycopeptides 430:442 arg2 intact glycopeptides intact glycopeptides glycopeptides Efficient and specific enrichment of intact glycopeptides could help greatly with this problem. 34105348 5 69 gly has 903:905 arg1 CpOGAD298N AND a GlcNAc specific binding characteristic CpOGAD298N a GlcNAc specific binding characteristic Cterm CpOGAD298N Q9EQQ9 Glycan array analysis and isothermal titration calorimetry (ITC) show that CpOGAD298N has a GlcNAc specific binding characteristic. 33924774 8 75 gly deglycosylation 1091:1105 arg1 ANTXR1 ANTXR1 PUBTATOR ANTXR1 84168 Cell blocking, co-immunoprecipitation, and plaque formation assays confirmed that deglycosylation of ANTXR1 prevents SVV attachment and subsequent entry. 32931036 7 63 gly Deglycosylated 964:977 arg1 Deglycosylated mGlu7 Deglycosylated mGlu7 Cterm Deglycosylated mGlu7 2917 Deglycosylated mGlu7 is retained in the ER, obstructing expression on the cell surface, and is degraded through the autophagolysosomal degradation pathway. 33889548 6 95 gly N-glycopeptides 1010:1024 arg2 site-specific intact N-glycopeptides PON1 N-glycopeptides PUBTATOR PON1 5444 N-glycopeptides Then, the alteration of site-specific intact N-glycopeptides of PON1 was comprehensively assessed by using Immunoprecipitation (IP) and mass spectrometry based 16O/18O C-terminal labeling quantification method to distinguish AFP-negative HCC from LC patients in a validation set (n = 64). 32653496 3 121 gly proteins 670:677 arg1 lipopolysaccharide proteins lipopolysaccharide Fterm proteins METHODS We performed an imaging-based, quantitative, high-throughput screen (using CaCo-2 and T84 cells incubated with lipopolysaccharide; tumor necrosis factor; histamine; receptor antagonists; and libraries of secreted proteins, microbial metabolites, and drugs) to identify molecules that altered epithelial tight junction (TJ) and focal adhesion morphology. 33065162 8 62 gly RBPR2 1248:1252 arg1 O-GlcNAcylation RBPR2 O-GlcNAcylation PUBTATOR RBPR2 74152 RESULTS Disruption of retinol cascade, RBP4 overproduction, O-GlcNAcylation of RBPR2, decreased RBP4 binding activity on RBPR2 and inflammation were found in livers of db/db and ob/ob mice and high glucose-cultured hepatocytes. 34327998 1 6 part_of sites 184:188 arg1 proteins proteins sites Fterm Site proteins sites A method for representing and comparing distributions of N-linked glycans located at specific sites on proteins is presented. 32221039 9 27 gly Fab-N-linked-glycosylation 1682:1707 arg2 Fab-N-linked-glycosylation introduction sites Fab-N-linked-glycosylation introduction sites sites Fab-N-linked-glycosylation introduction sites are observed in a minority of anti-NET B cell clones but can strongly influence NET-Ag binding. 31885260 7 20 gly sequence 1609:1616 arg1 the CpG repeat sequence the CpG repeat sequence Our data indicate that this highly fluorescent cyclic adduct adopts the promutagenic syn conformation and can stabilize the slipped mutagenic intermediate (SMI) within the CpG repeat of the NarI sequence, which is a hotspot for frameshift mutagenesis mediated by polycyclic N-linked C8-dG adducts. 34418796 0 38 gly glycosylation 8:20 arg1 the Ibaraki virus NS3 protein the Ibaraki virus NS3 protein Fterm protein Partial glycosylation of the Ibaraki virus NS3 protein is sufficient to support virus propagation. 33849982 3 32 gly glycoproteins 796:808 arg1 glycoproteins glycoproteins Cell wall polysaccharides are typically synthesized by Leloir glycosyltransferases, enzymes that are notoriously difficult to characterize, but their nucleotide-sugar substrates are well known and provide the opportunity to inspect the monosaccharides available for incorporation into cell wall polysaccharides and glycoproteins. 32103424 3 83 gly glycoproteins 706:718 arg1 their salivary glycoproteins glycoproteins the N/O-glycan profiles Fterm glycoproteins The present study validated alteration of salivary protein glycopatterns recognized by Bandeiraea simplicifolia lectin I (BS-I) in the saliva of patients with breast diseases using saliva microarrays, and the N/O-glycan profiles of their salivary glycoproteins isolated by the BS-I-magnetic particle conjugates from 259 female subjects (66 healthy volunteers (HV), 65 benign breast cyst or tumor patients (BB), 66 patients with breast cancer in stage I (BC-I) and 62 patients with breast cancer in stage II (BC-II)) were analyzed by MALDI-TOF/TOF-MS. 34644163 0 41 part_of C-Terminal 35:44 arg1 a Cargo Protein C-Terminal Domain Cargo Protein C a Cargo Protein C-Terminal Domain OGER Cargo Protein C P02810 N-Glycosylation of a Cargo Protein C-Terminal Domain Recognized by the Type IX Secretion System in Cytophaga hutchinsonii Affects Protein Secretion and Localization. 34644163 0 51 part_of Protein 27:33 arg1 a Cargo Protein C-Terminal Domain Cargo Protein C a Cargo Protein C-Terminal Domain OGER Cargo Protein C P02810 N-Glycosylation of a Cargo Protein C-Terminal Domain Recognized by the Type IX Secretion System in Cytophaga hutchinsonii Affects Protein Secretion and Localization. 34644163 0 109 part_of Cargo 21:25 arg1 a Cargo Protein C-Terminal Domain Cargo Protein C a Cargo Protein C-Terminal Domain OGER Cargo Protein C P02810 N-Glycosylation of a Cargo Protein C-Terminal Domain Recognized by the Type IX Secretion System in Cytophaga hutchinsonii Affects Protein Secretion and Localization. 35006749 4 46 gly glycosylated 580:591 arg1 glycosylated PD-L1 glycosylated PD-L1 PUBTATOR PD-L1 29126 Molecularly imprinted by PD-L1 N-glycans, NanoNiche can specifically recognize glycosylated PD-L1 on the tumor cell surface, thereby resulting in more efficient PD-L1 blockade. 33889548 6 19 part_of PON1 1029:1032 arg1 site-specific intact N-glycopeptides PON1 site-specific intact N-glycopeptides PUBTATOR Site PON1 5444 N-glycopeptides Then, the alteration of site-specific intact N-glycopeptides of PON1 was comprehensively assessed by using Immunoprecipitation (IP) and mass spectrometry based 16O/18O C-terminal labeling quantification method to distinguish AFP-negative HCC from LC patients in a validation set (n = 64). 32439055 2 30 gly glycopeptides 305:317 arg2 Seventeen N-linked glycopeptides Seventeen N-linked glycopeptides glycopeptides Seventeen N-linked glycopeptides from horseradish peroxidase (HRP) tryptic digests were readily identified and the results showed highly improved signal intensities. 32606389 0 9 gly glycoproteins 89:101 arg1 human glycoproteins human glycoproteins Fterm glycoproteins Author Correction: Mutually exclusive locales for N-linked glycans and disorder in human glycoproteins. 34968415 4 29 gly glycosylation 709:721 arg2 a nascent N-linked glycosylation site a nascent N-linked glycosylation site site Although this mutation generates a nascent N-linked glycosylation site, the additional N-linked glycan is dispensable for the virological property conferred by this mutation. 32878894 6 128 part_of ALV-J 1563:1567 arg1 the bipartite sequence motif ALV-J gp85 the bipartite sequence motif Cterm Site ALV-J gp85 motif Taken together, our findings indicated that the bipartite sequence motif, spanning aa 38 to 131 and aa 159 to 283, of ALV-J gp85 was essential for binding to chNHE1, with its two N-linked glycosylation sites and two cysteines being important for its receptor-binding function and subsequent viral infection steps.IMPORTANCE Infection of a cell by retroviruses requires the attachment and fusion of the host and viral membranes. 34724770 3 19 gly O-GlcNAc 387:394 arg1 O-GlcNAcylation O-GlcNAc O-GlcNAcylation PUBTATOR O-GlcNAc 8473 Similar to phosphorylation, O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) is involved in various cell-signaling processes, including apoptosis and proliferation, with O-GlcNAc addition and removal regulated by O-GlcNAc transferase and O-GlcNAcase (OGA), respectively. 31911580 4 5 gly threonine 594:602 arg1 O-GlcNAc threonine 255 O-GlcNAc threonine 255 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 5 gly threonine 594:602 arg1 O-linked N-acetylglucosamine threonine 255 O-linked N-acetylglucosamine threonine 255 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 phosphoglycerate kinase 1 AND O-GlcNAc phosphoglycerate kinase 1 O-GlcNAc PUBTATOR phosphoglycerate kinase 1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 PGK1 AND O-GlcNAc PGK1 O-GlcNAc PUBTATOR PGK1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 the first ATP-producing enzyme AND O-GlcNAc the first ATP-producing enzyme O-GlcNAc Fterm enzyme We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 phosphoglycerate kinase 1 AND O-linked N-acetylglucosamine phosphoglycerate kinase 1 O-linked N-acetylglucosamine PUBTATOR phosphoglycerate kinase 1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 PGK1 AND O-linked N-acetylglucosamine PGK1 O-linked N-acetylglucosamine PUBTATOR PGK1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 the first ATP-producing enzyme AND O-linked N-acetylglucosamine the first ATP-producing enzyme O-linked N-acetylglucosamine Fterm enzyme We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 35004852 6 35 gly glycoprotein 1085:1096 arg1 a flow-based glycoprotein synthesis system a flow-based glycoprotein synthesis system Fterm glycoprotein In this work, we demonstrate a flow-based glycoprotein synthesis system that promotes enhanced cell-free protein synthesis, efficient protein glycosylation with an immobilized oligosaccharyltransferase, and enrichment of the protein product from cell-free lysate. 34734627 0 10 gly N-glycosylation 56:70 arg1 an STT3B-dependent acceptor site an STT3B-dependent acceptor site site A cytosolic reductase pathway is required for efficient N-glycosylation of an STT3B-dependent acceptor site. 34458505 8 39 gly glycosylation 1699:1711 arg2 an N-linked glycosylation site an N-linked glycosylation site site The amino acid at this position is an N-linked glycosylation site, and mutation of the N-linked glycosylation is an immune escaped strategy adopted by this virus causing a persistent infection in the natural host. 34458505 3 21 part_of GP5 789:791 arg1 the GP5 gene sequence GP5 the GP5 gene sequence PUBTATOR Site GP5 P40197 sequence The present study was designed with the aim of isolation and characterization of the Betaarterivirus suid 2 from outbreaks in Mizoram in primary porcine alveolar macrophage and subsequently characterized the GP5 gene sequence of the isolate in terms of phylogenetic analysis and deduce amino acid sequence comparison. 34555499 10 70 gly glycoproteins 1951:1963 arg1 OC glycoproteins OC glycoproteins Cterm OC glycoproteins 3958 The data displays a complex sulfation pattern of O-glycans on OC glycoproteins and that aggressiveness of the cancer is associated with a decreased expression of the Gal3ST2 transferase. 31860302 2 71 gly glycoproteins 212:224 arg1 specific glycoproteins specific glycoproteins Fterm glycoproteins Certain distinctive glycoforms on specific glycoproteins enhance the specificity and/or sensitivity of cancer diagnosis. 33557755 7 12 part_of sequences 1207:1215 arg1 the highly variable region 1 sequences the highly variable region 1 region We tested the method on two datasets: a set of 156 founder and chronic gp160 HIV-1 subtype B sequences as well as a set of reference sequences of gp120 in the highly variable region 1. 33557755 7 15 part_of gp120 1220:1224 arg1 the highly variable region 1 gp120 the highly variable region 1 PUBTATOR Site gp120 3700 region We tested the method on two datasets: a set of 156 founder and chronic gp160 HIV-1 subtype B sequences as well as a set of reference sequences of gp120 in the highly variable region 1. 33557755 7 17 part_of gp120 1220:1224 arg1 reference sequences gp120 reference sequences PUBTATOR Site gp120 3700 sequences We tested the method on two datasets: a set of 156 founder and chronic gp160 HIV-1 subtype B sequences as well as a set of reference sequences of gp120 in the highly variable region 1. 33557755 7 60 part_of region 1249:1254 arg1 reference sequences region reference sequences sequences We tested the method on two datasets: a set of 156 founder and chronic gp160 HIV-1 subtype B sequences as well as a set of reference sequences of gp120 in the highly variable region 1. 32201074 9 62 part_of EGF 1142:1144 arg1 EGF domains EGF EGF domains OGER Site EGF P01133 domains Through in vitro experiments, we showed that O-GlcNAc mediates the stability of EGF domains in the same manner as O-fucose and O-glucose. 32002580 0 32 gly glycopeptide 64:75 arg2 N-linked glycopeptide enrichment N-linked glycopeptide enrichment glycopeptide Boronic acid functionalized MOFs as HILIC material for N-linked glycopeptide enrichment. 34559939 3 35 gly sialylated 552:561 arg1 multiple microvesicle-related sialylated proteins multiple microvesicle-related sialylated proteins Fterm proteins In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified multiple microvesicle-related sialylated proteins including CD63, a classic marker of exosomes. 34559939 3 35 gly sialylated 552:561 arg1 CD63 CD63 OGER CD63 P08962 In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified multiple microvesicle-related sialylated proteins including CD63, a classic marker of exosomes. 32222585 0 18 gly glycoforms 78:87 arg1 various IgG1 glycoforms various IgG1 glycoforms OGER IgG1 P01857 Glycosylation of Fcγ receptors influences their interaction with various IgG1 glycoforms. 32222585 0 46 gly Glycosylation 0:12 arg1 Fcγ receptors Fcγ receptors Fterm receptors Glycosylation of Fcγ receptors influences their interaction with various IgG1 glycoforms. 32002580 6 100 gly glycopeptides 1001:1013 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides The GO@UiO-66-PBA nanocomposite selectively enriches N-linked glycopeptides from tryptic digests of horseradish peroxidase (HRP) and immunoglobulin (IgG). 32046000 9 44 gly glycopeptide 1388:1399 arg2 Only the glycopeptide Only the glycopeptide glycopeptide Only the glycopeptide was recognized by anti-spore polyclonal immune serum demonstrating that the glycan moiety is a predominant spore-associated surface antigen. 33419227 4 13 gly N-glycosylation 514:528 arg2 N-glycosylation sites N-glycosylation sites sites In this study, we used the introduction of N-glycosylation sites as a tool to explore what effect this glycosylation has on the protein folding, secretion, and function of this key anticoagulant. 34725712 7 46 gly glycosite 1106:1114 arg2 Subsequent glycosite analysis Subsequent glycosite analysis glycosite Subsequent glycosite analysis revealed that alteration to the last four TPRs corresponded to the greatest shift in the resulting O-GlcNAc consensus sequence. 32002580 4 89 part_of MOF 578:580 arg1 The synthesized MOF nanocomposite MOF The synthesized MOF nanocomposite OGER Site MOF Q9H7Z6 nanocomposite The synthesized MOF nanocomposite is characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and BET. 32851535 0 29 gly glycopeptides 132:144 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides Gold nanoparticle-glutathione-functionalized porous graphene oxide-based hydrophilic beads for the selective enrichment of N-linked glycopeptides. 35822049 9 17 gly DNAJC6/AUXI 1619:1629 arg1 the O-GlcNAcylation DNAJC6 the O-GlcNAcylation PUBTATOR DNAJC6 9829 Significant changes in the O-GlcNAcylation of DNAJC6/AUXI, and PICALM, proteins that are risk factors for PD and/or AD respectively, were detected. 35822049 9 62 gly PICALM 1636:1641 arg1 the O-GlcNAcylation PICALM the O-GlcNAcylation PUBTATOR PICALM 8301 Significant changes in the O-GlcNAcylation of DNAJC6/AUXI, and PICALM, proteins that are risk factors for PD and/or AD respectively, were detected. 34483834 4 18 part_of PKR2 572:575 arg1 the N-terminal region PKR2 the N-terminal region PUBTATOR Site PKR2 246313 region Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. 31919391 1 8 gly N-glycopeptides 221:235 arg2 N-glycopeptides N-glycopeptides N-glycopeptides The development of methods to effectively capture N-glycopeptides from the complex biological samples is crucial to N-glycoproteome profiling. 34738170 8 15 gly glycoprotein 1730:1741 arg1 human glycoprotein human glycoprotein Fterm glycoprotein Collectively, our work not only sheds light on the functional relationships between GPI-anchored proteins and N-linked oligosaccharides of glycoproteins in S. pombe, but also supports the application of S. pombe to the production of human glycoprotein. 34738170 8 91 gly glycoproteins 1630:1642 arg1 glycoproteins glycoproteins N-linked oligosaccharides Fterm glycoproteins Collectively, our work not only sheds light on the functional relationships between GPI-anchored proteins and N-linked oligosaccharides of glycoproteins in S. pombe, but also supports the application of S. pombe to the production of human glycoprotein. 34520755 1 31 part_of has 202:204 arg1 a neutral pH-active hyaluronidase AND six potential N-glycosylation sites a neutral pH-active hyaluronidase six potential N-glycosylation sites Fterm Site hyaluronidase sites rHuPH20, a neutral pH-active hyaluronidase that degrades glycosaminoglycans under physiologic conditions, has six potential N-glycosylation sites. 33451024 4 13 part_of HA2 758:760 arg1 HA2 domain HA2 HA2 domain OGER Site HA2 domain Results from NetNGlyc program analyzed the H5 hemagglutinin sequences of isolates during 2004-2020, revealing that seven and two conserved N-glycosylation sites were detected in HA1 and HA2 domain, respectively. 34829055 6 9 gly used 1246:1249 arg2 residue-SDF residue-SDF and residue-IDF residue-SDF and residue-IDF In summary, this study suggests that the residue-SDF and residue-IDF could be used as the ideal dietary fibers for application in the functional food industry. 33469950 3 43 gly glycoproteins 407:419 arg1 different N-linked glycans glycoproteins different N-linked glycans Fterm glycoproteins Three forms of SVLLP were purified from mouse seminal vesicle secretions and characterized as glycoproteins with the same protein core but different N-linked glycans. 32193337 8 44 gly glycosylation 1236:1248 arg1 AHCY AHCY PUBTATOR AHCY 269378 In addition, blocking glycosylation of AHCY reduces somatic cell reprogramming. 34668190 11 97 gly p65 1878:1880 arg1 O-GlcNAcylation p65 O-GlcNAcylation PUBTATOR p65 19697 OGT deficiency attenuated the O-GlcNAcylation, activity, and nuclear translocation of p65, resulting in downregulation of IL-6 in mouse skeletal muscle of mice exposed to cold conditions. 33242079 0 104 gly alpha2,6-sialylated 12:30 arg1 alpha2,6-sialylated glycans alpha2,6-sialylated glycans Majority of alpha2,6-sialylated glycans in the adult mouse brain exist in O-glycans: SALSA-MS analysis for knockout mice of alpha2,6-sialyltransferase genes. 34644163 0 118 gly N-Glycosylation 0:14 arg1 a Cargo Protein C-Terminal Domain a Cargo Protein C-Terminal Domain Fterm Domain N-Glycosylation of a Cargo Protein C-Terminal Domain Recognized by the Type IX Secretion System in Cytophaga hutchinsonii Affects Protein Secretion and Localization. 33665237 10 19 gly glycoproteins 1848:1860 arg1 the small integrin-binding ligand N-linked glycoproteins the small integrin-binding ligand N-linked glycoproteins Cterm N-linked glycoproteins 25333 This study focused primarily on the NCPs belonging to the small leucine-rich proteoglycan (SLRP) family and the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). 33665237 10 72 gly leucine-rich 1761:1772 arg1 the small leucine-rich proteoglycan (SLRP) family leucine the small leucine-rich proteoglycan (SLRP) family leucine This study focused primarily on the NCPs belonging to the small leucine-rich proteoglycan (SLRP) family and the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). 33924774 5 13 part_of ANTXR1 650:655 arg1 the ANTXR1 vWA domain ANTXR1 the ANTXR1 vWA domain PUBTATOR Site ANTXR1 84168 domain Here we show that N-linked glycosylation in the ANTXR1 vWA domain is necessary for SVV attachment and entry. 33863420 2 45 gly glycopeptides 681:693 arg2 glycopeptides glycopeptides glycopeptides The composite not only exhibited strong affinity for phosphopeptide due to the coexistence of Zr-O clusters and Ti-O clusters, but also owned great hydrophilicity for glycopeptides relying on abundant hydrophilic NH2 groups, meeting the demand for simultaneously enrichment and sequential elution of phosphopeptides and glycopeptides. 33863420 2 75 gly glycopeptides 528:540 arg2 glycopeptides glycopeptides glycopeptides The composite not only exhibited strong affinity for phosphopeptide due to the coexistence of Zr-O clusters and Ti-O clusters, but also owned great hydrophilicity for glycopeptides relying on abundant hydrophilic NH2 groups, meeting the demand for simultaneously enrichment and sequential elution of phosphopeptides and glycopeptides. 34050865 3 5 gly glycoproteins 629:641 arg1 many N-linked glycoproteins many N-linked glycoproteins Fterm glycoproteins The lectin-like chaperones calreticulin and calnexin are an important class of structurally-related chaperones relevant for the folding and assembly of many N-linked glycoproteins. 34469835 0 9 part_of Impact 0:5 arg1 chemical composition Impact chemical composition OGER Site Impact Q9P2X3 position Impact of fermentation temperature and grape solids content on the chemical composition and sensory profiles of Cabernet Sauvignon wines made from flash détente treated must fermented off-skins. 32117864 10 100 gly glycosites 1787:1796 arg2 glycosites glycosites glycosites A notable aspect of our analysis of metabolically glycoengineered breast cells was decreased sialylation of a subset of glycosites, which was unexpected because of the increased intracellular levels of sialometabolite "building blocks" in the 1,3,4-O-Bu3ManNAc-treated cells. 34105348 1 60 part_of threonine 237:245 arg1 proteins proteins threonine Fterm AminoAcid proteins threonine O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 34105348 1 64 part_of serine 227:232 arg1 proteins proteins serine Fterm AminoAcid proteins serine O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 34322281 11 27 part_of enzyme 1587:1592 arg1 enzyme residues K281 and K363 enzyme enzyme residues K281 and K363 Fterm Site enzyme residues For the +1 position of the peptide, enzyme residues K281 and K363 formed gating interactions with aromatics and glutamines at the +1 position of the peptide, leading to modes of peptide-binding sub-optimal for catalysis. 32886756 5 57 gly neoglycopeptide 1374:1388 arg2 neoglycopeptide synthesis neoglycopeptide synthesis neoglycopeptide Maf glycosyltransferases have the potential to radically expand the scope of neoglycopeptide synthesis and posttranslational protein engineering. 32046000 4 0 gly protein 847:853 arg1 the central, collagen repeat region protein the central, collagen repeat region Fterm protein These peptide sequences were selected from the central, collagen repeat region of the BclA3 protein. 33436086 2 26 part_of glycoprotein 487:498 arg1 position 158 glycoprotein position 158 Fterm Site glycoprotein position 158 We have shown earlier that lack of glycosylation at position 158 of the hemagglutinin (HA) glycoprotein due to the T160A mutation is a key determinant of the dual receptor binding property of clade 2.3.4.4 H5NX subtypes. 33997889 8 54 part_of ComP 1141:1144 arg1 >100 different ComP fragments ComP >100 different ComP fragments OGER Site ComP P49747 fragments We tested >100 different ComP fragments individually fused to Pseudomonas aeruginosa exotoxin A (EPA), leading to the identification of an 11-amino acid sequence sufficient for robust glycosylation by PglS. 33024038 15 110 gly glycoproteins 2704:2716 arg1 multiple O-linked host glycoproteins multiple O-linked host glycoproteins Fterm glycoproteins We propose that CpaA belongs to an expanding class of functionally defined glycoproteases that targets multiple O-linked host glycoproteins. 33906120 4 70 gly glycosylated 603:614 arg1 308 glycosylated peptides 308 glycosylated peptides peptides In total, we detected 308 glycosylated peptides and 316 glycosylated sites corresponding to 248 unique glycoproteins. 33906120 4 84 gly glycoproteins 680:692 arg1 248 unique glycoproteins 248 unique glycoproteins Fterm glycoproteins In total, we detected 308 glycosylated peptides and 316 glycosylated sites corresponding to 248 unique glycoproteins. 33906120 4 87 gly glycosylated 633:644 arg1 316 glycosylated sites 316 glycosylated sites sites In total, we detected 308 glycosylated peptides and 316 glycosylated sites corresponding to 248 unique glycoproteins. 31904283 8 23 gly O-glycoprotein 1084:1097 arg1 the O-glycoprotein podoplanin the O-glycoprotein podoplanin Fterm O-glycoprotein Expression of the O-glycoprotein podoplanin was lost, while Tn antigen, representing immature O-glycans, was most abundantly found on podocalyxin. 31904283 8 61 gly found 1191:1195 arg1 podocalyxin AND Tn antigen podocalyxin Tn antigen PUBTATOR podocalyxin 27205 Expression of the O-glycoprotein podoplanin was lost, while Tn antigen, representing immature O-glycans, was most abundantly found on podocalyxin. 35517525 0 19 gly protein 112:118 arg1 functionalized N-linked glycan polymers protein functionalized N-linked glycan polymers Fterm protein Synthesis of aryl azide chain-end functionalized N-linked glycan polymers and their photo-labelling of specific protein. 33924503 9 56 part_of glycoproteins 1349:1361 arg1 glycoside composition glycoproteins glycoside composition Fterm Site glycoproteins position Changes in glycoside composition of glycoproteins on the platelets' surface impaired their functional capacity and increased their apoptosis. 34725712 0 14 gly glycosite 57:65 arg2 glycosite glycosite glycosite Truncation of the TPR domain of OGT alters substrate and glycosite selection. 33335689 5 11 gly O-glycosylation 896:910 arg2 all potential O-glycosylation sites all potential O-glycosylation sites sites Surprisingly, O-glycans could be formed at new sites when an initial O-glycosylation site was eliminated, and continued to occur until all potential O-glycosylation sites were nulled. 33335689 5 68 gly O-glycosylation 816:830 arg2 an initial O-glycosylation site an initial O-glycosylation site site Surprisingly, O-glycans could be formed at new sites when an initial O-glycosylation site was eliminated, and continued to occur until all potential O-glycosylation sites were nulled. 33682335 1 9 gly glycoproteins 258:270 arg1 glycoproteins glycoproteins Fterm glycoproteins An understanding of why hydrophilic interaction liquid chromatography gives a higher resolution for glycans than for glycoproteins would facilitate column improvements. 34227365 1 62 gly N-Glycosylation 94:108 arg1 proteins proteins Fterm proteins N-Glycosylation of proteins, an important post-translational modification in eukaryotic cells, plays an essential role in the regulation of cell adhesion, migration, signal transduction, and apoptosis. 33733058 0 40 gly proteins 20:27 arg1 Sialylation proteins Sialylation Fterm proteins Sialylation of host proteins as targetable risk factor for COVID-19 susceptibility and spreading: A hypothesis. 33950562 8 0 gly JunB 1131:1134 arg1 Target protein O-GlcNAcylation JunB Target protein O-GlcNAcylation OGER JunB P17275 Basic Protocol 1: Target protein O-GlcNAcylation of JunB using nanobody-OGT Basic Protocol 2: Target protein deglycosylation of Nup62 using nanobody-splitOGA Alternate Protocol: Verification of the O-GlcNAc state of a tagged target protein through chemoenzymatic labeling Support Protocol: Cloning of new nanobody-OGT/nanobody-splitOGA and target protein pairs. 33950562 8 54 gly deglycosylation 1188:1202 arg1 Nup62 Nup62 OGER Nup62 P37198 Basic Protocol 1: Target protein O-GlcNAcylation of JunB using nanobody-OGT Basic Protocol 2: Target protein deglycosylation of Nup62 using nanobody-splitOGA Alternate Protocol: Verification of the O-GlcNAc state of a tagged target protein through chemoenzymatic labeling Support Protocol: Cloning of new nanobody-OGT/nanobody-splitOGA and target protein pairs. 33950562 8 57 gly protein 1311:1317 arg1 the O-GlcNAc state protein the O-GlcNAc state Fterm protein Basic Protocol 1: Target protein O-GlcNAcylation of JunB using nanobody-OGT Basic Protocol 2: Target protein deglycosylation of Nup62 using nanobody-splitOGA Alternate Protocol: Verification of the O-GlcNAc state of a tagged target protein through chemoenzymatic labeling Support Protocol: Cloning of new nanobody-OGT/nanobody-splitOGA and target protein pairs. 31859482 4 7 gly derived 750:756 arg2 N-linked glycoproteins AND glycans N-linked glycoproteins glycans Fterm glycoproteins To address the limitation, we developed a high-throughput method for characterizing glycans, glycosites, and intact glycopeptides (IGPs) derived from N-linked glycoproteins. 31859482 4 13 gly glycosites 706:715 arg2 glycosites glycosites glycosites To address the limitation, we developed a high-throughput method for characterizing glycans, glycosites, and intact glycopeptides (IGPs) derived from N-linked glycoproteins. 31859482 4 17 gly glycoproteins 772:784 arg1 N-linked glycoproteins N-linked glycoproteins Fterm glycoproteins To address the limitation, we developed a high-throughput method for characterizing glycans, glycosites, and intact glycopeptides (IGPs) derived from N-linked glycoproteins. 31859482 4 66 gly glycopeptides 729:741 arg2 intact glycopeptides intact glycopeptides glycopeptides To address the limitation, we developed a high-throughput method for characterizing glycans, glycosites, and intact glycopeptides (IGPs) derived from N-linked glycoproteins. 34408013 3 26 part_of IgG-Fab 373:379 arg1 the IgG-Fab regions Fab the IgG-Fab regions PUBTATOR Site Fab 2187 regions This is induced after target recognition through the IgG-Fab regions, allowing neighboring IgG-Fc tails to associate through Fc:Fc interaction, ultimately leading to hexamer formation. 34408013 3 46 part_of IgG-Fc 411:416 arg1 neighboring IgG-Fc tails IgG neighboring IgG-Fc tails Cterm Site IgG tails This is induced after target recognition through the IgG-Fab regions, allowing neighboring IgG-Fc tails to associate through Fc:Fc interaction, ultimately leading to hexamer formation. 34215103 4 84 gly glycopeptide 945:956 arg2 N-linked glycopeptide N-linked glycopeptide glycopeptide Thanks to the suitable pore channel structure as well as strong hydrophilic surface, the as-prepared MPDA@PEI@CA nanoparticles exhibited prominent performance in enrichment of N-linked glycopeptide with ultrahigh selectivity (1:5000 M ratio of horseradish peroxidase (HRP) digests/bovine serum albumin (BSA) digests), low detection limit (5 fmol μL-1), outstanding size-exclusion ability (1:1000 mass of HRP/BSA), and unique reusability (five times). 34452648 11 71 part_of C1GALT1 1431:1437 arg1 the C1GALT1 promoter region C1GALT1 the C1GALT1 promoter region PUBTATOR Site C1GALT1 56913 region Moreover, transcription factor SP1 was found to bind to the C1GALT1 promoter region and activated its expression. 32498907 4 57 gly glycopeptides 930:942 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides After modified with glutathione by photo-initiated thiol-ene reaction, these materials exhibited hydrophilicity and could be used as adsorbents of HILIC for capturing N-linked glycopeptides from IgG and serum protein tryptic digests. 33333092 6 10 part_of acetyltransferase 1186:1202 arg1 pseudo histone acetyltransferase domain acetyltransferase pseudo histone acetyltransferase domain Fterm Site acetyltransferase domain By applying a truncated OGA construct, we found that OGA's N-terminal region or pseudo histone acetyltransferase domain is not required for its O-GlcNAcylation, suggesting OGT functionally interacts with OGA through its catalytic and/or stalk domains. 32910214 0 69 gly multi-glycosylated 79:96 arg1 mono- and multi-glycosylated peptides/proteins mono- and multi-glycosylated peptides/proteins peptides/proteins Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach. 31904283 1 80 gly T 241:241 arg1 C1GalT1 T synthase C1GalT1 PUBTATOR T synthase 94192 Mucin-type O-linked glycosylation, a posttranslational modification affecting the stability and biophysical characteristics of proteins, requires C1GalT1 (T synthase) and its obligate, X-linked chaperone Cosmc. 34335620 6 58 gly glycoprotein 1298:1309 arg1 glycoprotein glycoprotein Fterm glycoprotein Here we exploited both single-cell antibody gene sequencing and proteomic sequencing approaches to assess the composition of the ebolavirus glycoprotein (GP)-reactive antibody repertoire in the plasma of an EVD survivor. 33608772 11 6 gly glycosylated 1774:1785 arg1 serum-IgA1 serum-IgA1 PUBTATOR IgA1 P01876 This specific glycosylated form of serum-IgA1 will expand the spectrum of aberrant glycosylation which provides valuable information to cancer glycobiology. 34105348 0 37 gly Protein 42:48 arg1 O-GlcNAcylation Quantification Protein O-GlcNAcylation Quantification Fterm Protein O-GlcNAcylation Quantification of Certain Protein by the Proximity Ligation Assay and Clostridium perfringen OGAD298N(CpOGAD298N). 33728790 3 37 gly glycoproteins 631:643 arg1 differentially expressed glycoproteins differentially expressed glycoproteins Fterm glycoproteins We conducted a glycoproteomic analysis of P. sojae to identify and map N-glycosylated proteins and to quantify differentially expressed glycoproteins associated with mycelia, asexual cyst, and sexual oospore developmental stages. 33728790 3 69 gly N-glycosylated 566:579 arg1 N-glycosylated proteins N-glycosylated proteins Fterm proteins We conducted a glycoproteomic analysis of P. sojae to identify and map N-glycosylated proteins and to quantify differentially expressed glycoproteins associated with mycelia, asexual cyst, and sexual oospore developmental stages. 33523898 1 52 gly glycosylation 141:153 arg1 ER ER PUBTATOR ER 2069 Asparagine (N)-linked glycosylation is required for endoplasmic reticulum (ER) homeostasis, but how this co- and posttranslational modification is maintained during ER stress is unknown. 31936366 0 37 gly c-Myc 29:33 arg1 Increased O-GlcNAcylation Myc Increased O-GlcNAcylation OGER Myc P01108 Increased O-GlcNAcylation of c-Myc Promotes Pre-B Cell Proliferation. 32337418 3 40 gly glycoforms 665:674 arg1 murine IgG Fc glycoforms murine IgG Fc glycoforms PUBTATOR IgG 16059 In this study, we established a method to quantify murine IgG Fc glycoforms normalized to the protein abundance at a subclass-specific level for various mouse strains using multiple reaction monitoring. 33167279 4 41 part_of multi-functional 754:769 arg1 a multi-functional peptide multi a multi-functional peptide OGER Site multi peptide Herein, a label-free and sensitive SPR biosensor was developed for accurate detection of OGT based on a multi-functional peptide. 31860302 9 70 gly glycopeptides 1567:1579 arg2 plasma intact glycopeptides plasma intact glycopeptides glycopeptides These results suggest that this method will be useful for analyzing plasma intact glycopeptides in future studies. 34677046 8 36 gly glycosites 1236:1245 arg2 Novel glycosites Novel glycosites glycosites Novel glycosites were confirmed using parallel multiprotease digestion, LC-MS/MS, and FAIMS-MS to define the glycoproteomes of WT and ΔpglB::pglBC. 33647064 5 57 part_of GPC 609:611 arg1 the ectodomain GPC the ectodomain PUBTATOR Site GPC P04921 ectodomain In a lethal animal model of hemorrhagic fever-causing Machupo virus (MACV) infection, we found that a chimeric MACV with the ectodomain of GPC from Candid#1 vaccine was partially attenuated. 34359264 11 81 part_of pigs 1612:1615 arg1 microbiota composition pigs microbiota composition OGER Site pigs Q96S52 position Although the use of PSB did not modify the overall richness of microbiota composition of these slaughter pigs, it may have increased specific taxa associated with better gut health parameters. 33535865 1 41 gly glycoprotein 176:187 arg1 O-linked glycoprotein glycans O-linked glycoprotein glycans OBJECTIVE This study aimed to investigate the expression of O-linked glycoprotein glycans in tissue of patients with cholangiocarcinoma compared with adjacent normal tissue. 34677377 6 50 gly fucosylated 1185:1195 arg1 fucosylated glycans fucosylated glycans Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, p-value < 0.001) indicating cleavage of fucosylated glycans in milk at room temperature. 34227365 21 19 gly N-glycoproteins 3527:3541 arg1 175 significantly down-regulated N-glycoproteins 175 significantly down-regulated N-glycoproteins Fterm N-glycoproteins In females, we found 175 significantly down-regulated N-glycoproteins and 31 significantly up-regulated N-glycoproteins with respect to males. 34227365 21 116 gly N-glycoproteins 3577:3591 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins In females, we found 175 significantly down-regulated N-glycoproteins and 31 significantly up-regulated N-glycoproteins with respect to males. 34056367 3 58 gly residue 550:556 arg1 the oligosaccharide donor asparagine residue the oligosaccharide donor asparagine residue The enzyme catalyzes glycosidic bond formation between the oligosaccharide donor, containing nucleoside phosphatase, and the amide nitrogen of the asparagine residue. 32408838 0 20 gly saponin 19:25 arg1 nine sugar units saponin nine sugar units Fterm saponin A new triterpenoid saponin with nine sugar units from Anemone amurensis (Korsh.) 33682335 0 14 gly glycoproteins 77:89 arg1 glycoproteins glycoproteins Fterm glycoproteins Protein-induced conformational change in glycans decreases the resolution of glycoproteins in hydrophilic interaction liquid chromatography. 32493725 1 123 part_of protein 218:224 arg1 Deposition tau protein Deposition PUBTATOR Site tau protein 4137 position Deposition of hyperphosphorylated and aggregated tau protein in the central nervous system is characteristic of Alzheimer disease and other tauopathies. 34327998 7 31 gly glycopeptide 1214:1225 arg2 glycopeptide identity glycopeptide identity glycopeptide Since GADS contain only MS1 abundances and identifications, they are equally suitable for expressing collision-induced fragmentation and electron-transfer dissociation determinations of glycopeptide identity. 32498907 1 2 gly glycopeptides 329:341 arg2 glycopeptides enrichment glycopeptides enrichment glycopeptides Hydrophilic interaction chromatography (HILIC), as one of the important methods for glycopeptides enrichment, has attracted the attention of more and more researchers due to the high efficiency in glycopeptides enrichment. 32498907 1 18 gly glycopeptides 216:228 arg2 glycopeptides enrichment glycopeptides enrichment glycopeptides Hydrophilic interaction chromatography (HILIC), as one of the important methods for glycopeptides enrichment, has attracted the attention of more and more researchers due to the high efficiency in glycopeptides enrichment. 33552834 3 20 part_of receptors 621:629 arg1 sequence 16 ACE2 receptors sequence PUBTATOR Site 16 ACE2 receptors Q9BYF1 sequence To understand the susceptibility of animals for infection and transmission, we did sequence and structure-based molecular interaction analysis of 16 ACE2 receptors from different mammalian species with SARS-CoV-2 spike 2 receptor binding domain. 33552834 3 101 part_of receptor 688:695 arg1 2 receptor binding domain spike 2 receptor 2 receptor binding domain PUBTATOR Site spike 2 receptor 43740568 domain To understand the susceptibility of animals for infection and transmission, we did sequence and structure-based molecular interaction analysis of 16 ACE2 receptors from different mammalian species with SARS-CoV-2 spike 2 receptor binding domain. 32117864 8 10 gly glycosylation 1437:1449 arg1 these proteins these proteins Fterm proteins A third category-protein folding chaperones-was unexpected because little precedent exists for the role of glycosylation in the activity of these proteins. 33197187 2 35 gly proteins 391:398 arg1 O-GlcNAc proteins O-GlcNAc Fterm proteins O-GlcNAcase (OGA) is the only enzyme that removes O-linked N-acetyl-d-glucosamine (O-GlcNAc) from target proteins. 33197187 2 35 gly proteins 391:398 arg1 O-linked N-acetyl-d-glucosamine proteins O-linked N-acetyl-d-glucosamine Fterm proteins O-GlcNAcase (OGA) is the only enzyme that removes O-linked N-acetyl-d-glucosamine (O-GlcNAc) from target proteins. 34711323 4 54 gly glycopeptide 511:522 arg2 released glycan and glycopeptide analysis released glycan and glycopeptide analysis glycopeptide Owing to the complexity of the intact protein, information about EPO glycosylation is commonly derived from released glycan and glycopeptide analysis using mass spectrometry (MS). 33419227 3 28 gly N-glycosylation 348:362 arg2 four potential N-glycosylation sites four potential N-glycosylation sites sites Antithrombin, a serpin with four potential N-glycosylation sites, plays a pivotal role in hemostasis, wherein its deficiency significantly increases thrombotic risk. 34681302 4 2 gly SeCMP 875:879 arg1 C. militaris polysaccharides CMP C. militaris polysaccharides OGER CMP Mice were treated with a normal diet (CHOW), HFD alone, HFD plus C. militaris polysaccharides (CMP), or low/medium/high dosage of SeCMP for 8 weeks. 31919391 0 63 gly glycopeptides 156:168 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides Facile synthesis of hydrophilic magnetic graphene nanocomposites via dopamine self-polymerization and Michael addition for selective enrichment of N-linked glycopeptides. 33914740 4 32 gly glycosylation 741:753 arg1 the rBer e 1 the rBer e 1 PUBTATOR rBer e 1 74915 We also determined whether this fungal glycosylation could affect the antigenicity and immunogenicity of the rBer e 1 by using dendritic cells (DC) as an immune cell model due to their role in modulating the immune response. 34327998 8 63 part_of sites 1276:1280 arg1 several proteins proteins sites Fterm Site proteins sites Comparisons of GADS for N-glycosylated sites on several proteins, especially the SARS-CoV-2 spike protein, demonstrate the potential reproducibility of GADS and their utility for comparing site-specific distributions. 34327998 8 63 part_of sites 1276:1280 arg1 the SARS-CoV-2 spike protein spike protein sites PUBTATOR Site spike protein 43740568 sites Comparisons of GADS for N-glycosylated sites on several proteins, especially the SARS-CoV-2 spike protein, demonstrate the potential reproducibility of GADS and their utility for comparing site-specific distributions. 34260942 3 57 gly protein 617:623 arg1 O-GlcNAcylation protein O-GlcNAcylation Fterm protein Here, we identify O-GlcNAcylation of proteasome activator subunit 3 (Psme3) protein as a node of the ESC pluripotency network. 32598381 4 91 gly Gly- 668:671 arg1 the 245 NA glycan Gly- the 245 NA glycan Cterm Gly- To assess the effect this novel N-linked glycan has on virus fitness and antibody binding, recombinant viruses with (NA Gly+) or without (NA Gly-) the 245 NA glycan were created. 32629760 0 3 gly Intake 13:18 arg1 Body Composition Body Composition Body Composition Site position Carbohydrate Intake in Early Childhood and Body Composition and Metabolic Health: Results from the Generation R Study. 32898702 1 76 part_of protein 185:191 arg1 Reversible glycosylation polypeptide protein Reversible glycosylation polypeptide Fterm Site protein polypeptide Reversible glycosylation polypeptide (RGP) is a type of plant-specific protein, primarily involved in the biosynthesis of cell wall polysaccharides, which in turn changes the shape of the cell walls and affects the wood properties of plants. 32065175 1 4 gly glycopeptide 79:90 arg2 Intact glycopeptide analysis Intact glycopeptide analysis glycopeptide Intact glycopeptide analysis is becoming more common with developments in mass spectrometry instrumentation and fragmentation approaches. 32518939 8 57 gly modified 1415:1422 arg1 isolated PAMR1 EGF-LD AND the three O-linked sugars isolated PAMR1 EGF-LD the three O-linked sugars PUBTATOR PAMR1 EGF 210622 Using click chemistry and mass spectrometry, isolated PAMR1 EGF-LD was demonstrated to be modified by the three O-linked sugars. 33206527 9 163 gly CES1 1788:1791 arg1 N79 glycan CES1 N79 glycan PUBTATOR CES1 1066 Given that the N-linked glycosyl group at Asn79 (N79 glycan) of CES1 is known to influence CES1 enzyme activity, we hypothesized that the post-translational modification of CES1 at N79 may be linked to its antiproliferative activity. 34677046 6 34 gly glycosylated 1023:1034 arg1 Peptides Peptides Peptides with novel sequons were either unaltered (likely not glycosylated) or showed abundance consistent with known glycopeptides. 34677046 6 78 gly glycopeptides 1079:1091 arg2 known glycopeptides known glycopeptides glycopeptides Peptides with novel sequons were either unaltered (likely not glycosylated) or showed abundance consistent with known glycopeptides. 32801002 1 28 gly proteins 232:239 arg1 the O-GlcNAcylation proteins the O-GlcNAcylation Fterm proteins The vital enzyme O-linked β-N-acetylglucosamine transferase (OGT) catalyzes the O-GlcNAcylation of intracellular proteins coupling the metabolic status to cellular signaling and transcription pathways. 32123732 2 5 gly glycoproteins 357:369 arg1 glycoproteins glycoproteins Fterm glycoproteins Despite advances in methods to produce defined glycoproteins by chemoenzymatic elaboration of monosaccharides, the understanding and engineering of glycoproteins remain challenging, in part, due to the difficulty of site-specifically controlling glycosylation at each of several positions within a protein. 32123732 2 49 gly glycoproteins 256:268 arg1 defined glycoproteins defined glycoproteins Fterm glycoproteins Despite advances in methods to produce defined glycoproteins by chemoenzymatic elaboration of monosaccharides, the understanding and engineering of glycoproteins remain challenging, in part, due to the difficulty of site-specifically controlling glycosylation at each of several positions within a protein. 32123732 2 21 gly glycosylation 455:467 arg2 several positions positions positions Despite advances in methods to produce defined glycoproteins by chemoenzymatic elaboration of monosaccharides, the understanding and engineering of glycoproteins remain challenging, in part, due to the difficulty of site-specifically controlling glycosylation at each of several positions within a protein. 33802415 1 20 gly glycoprotein 161:172 arg1 the glycoprotein hormone family the glycoprotein hormone family Fterm glycoprotein Follitropin (FSH) is a heterodimeric protein composed of an α subunit that is shared with the glycoprotein hormone family, including lutropin (LH), thyrotropin (TSH), human choriogonadotropin (hCG), and a unique β specific subunit. 33647395 3 18 gly glycoproteins 350:362 arg1 Small integrin-binding ligand N-linked glycoproteins Small integrin-binding ligand N-linked glycoproteins Fterm glycoproteins Dentin sialophosphoprotein (DSPP), a member of the SIBLING (Small integrin-binding ligand N-linked glycoproteins) family, has been reported to contribute to the proliferation and migration of different types of tumor cells, including glioma. 34342946 6 21 part_of butyrophilin 1101:1112 arg1 the N-terminal bovine butyrophilin subdomain butyrophilin the N-terminal bovine butyrophilin subdomain Fterm Site butyrophilin subdomain While there is less structural resemblance to members of the human butyrophilin family such as BTN3A, which play a role as immune receptors, the N-terminal bovine butyrophilin subdomain shows surprising similarity to the human myelin oligodendrocyte glycoprotein, a protein exposed on the surface of myelin sheaths. 32672529 9 8 gly A3α 1589:1591 arg1 Ser Ser Ser Cell-wall peptidoglycan was of type A3α l-Lys-Gly3 (Ser; similar to A11.2 and A11.3). 33182731 5 19 gly proteins 1248:1255 arg1 sialylation proteins sialylation Fterm proteins Further, utilizing enhanced intact glycopeptide identification afforded by the NGAG workflow, we found that the sugar analog 1,3,4-O-Bu3ManNAc, a "high flux" metabolic precursor for sialic acid biosynthesis, increased sialylation of secreted proteins including recombinant human erythropoietin (rhEPO). 33182731 5 21 gly glycopeptide 1041:1052 arg2 enhanced intact glycopeptide identification enhanced intact glycopeptide identification glycopeptide Further, utilizing enhanced intact glycopeptide identification afforded by the NGAG workflow, we found that the sugar analog 1,3,4-O-Bu3ManNAc, a "high flux" metabolic precursor for sialic acid biosynthesis, increased sialylation of secreted proteins including recombinant human erythropoietin (rhEPO). 33182731 5 26 gly sialylation 1224:1234 arg1 secreted proteins secreted proteins Fterm proteins Further, utilizing enhanced intact glycopeptide identification afforded by the NGAG workflow, we found that the sugar analog 1,3,4-O-Bu3ManNAc, a "high flux" metabolic precursor for sialic acid biosynthesis, increased sialylation of secreted proteins including recombinant human erythropoietin (rhEPO). 33182731 5 26 gly sialylation 1224:1234 arg1 recombinant human erythropoietin recombinant human erythropoietin PUBTATOR erythropoietin 2056 Further, utilizing enhanced intact glycopeptide identification afforded by the NGAG workflow, we found that the sugar analog 1,3,4-O-Bu3ManNAc, a "high flux" metabolic precursor for sialic acid biosynthesis, increased sialylation of secreted proteins including recombinant human erythropoietin (rhEPO). 34644163 15 92 gly N-glycosylation 2211:2225 arg1 C. hutchinsonii proteins C. hutchinsonii proteins Fterm proteins Glycosyltransferase CHU_3842 was found to participate in the N-glycosylation of C. hutchinsonii proteins and had effects on cell resistance to some chemicals, cell motility, and cellulose degradation. 34284575 4 24 gly O-glycosylation 495:509 arg2 only a few O-glycosylation sites only a few O-glycosylation sites sites Unlike the 22 confirmed N-glycosylation sites on SARS-CoV-2 S, only a few O-glycosylation sites on this protein have been reported. 34284575 4 69 gly N-glycosylation 445:459 arg2 the 22 confirmed N-glycosylation sites the 22 confirmed N-glycosylation sites sites Unlike the 22 confirmed N-glycosylation sites on SARS-CoV-2 S, only a few O-glycosylation sites on this protein have been reported. 34284575 7 5 gly glycopeptide 963:974 arg2 O-linked glycopeptide enrichment O-linked glycopeptide enrichment glycopeptide And WR-SiO2 can resist the nonglycopeptides' interference with the 100 molar fold of BSA during O-linked glycopeptide enrichment. 34284575 7 49 gly nonglycopeptides 885:900 arg2 the nonglycopeptides' the nonglycopeptides' nonglycopeptides And WR-SiO2 can resist the nonglycopeptides' interference with the 100 molar fold of BSA during O-linked glycopeptide enrichment. 31969392 0 52 gly sialylated 13:22 arg1 Sulfated and sialylated N-glycans Sulfated and sialylated N-glycans Sulfated and sialylated N-glycans in the echinoderm Holothuria atra reflect its marine habitat and phylogeny. 32676596 6 3 gly F-glycoprotein 1214:1227 arg1 F-glycoprotein F-glycoprotein Fterm F-glycoprotein Similarly, F-glycoprotein trimers from human parainfluenza virus-type 3 and spike-glycoprotein trimers from SARS-CoV-2 could be displayed on LuS nanoparticles with decent yield and antigenicity. 32676596 6 24 gly spike-glycoprotein 1279:1296 arg1 spike-glycoprotein spike-glycoprotein PUBTATOR spike 43740568 Similarly, F-glycoprotein trimers from human parainfluenza virus-type 3 and spike-glycoprotein trimers from SARS-CoV-2 could be displayed on LuS nanoparticles with decent yield and antigenicity. 32892942 0 39 gly glycopeptides 91:103 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides One-step synthesis of hydrophilic microspheres for highly selective enrichment of N-linked glycopeptides. 34127633 8 27 part_of flavoprotein 1293:1304 arg1 inhibitory methylation site electron transfer flavoprotein inhibitory methylation site OGER Site electron transfer flavoprotein site Western blotting determined the protein abundance of electron transfer flavoprotein (ETF) subunits, including inhibitory methylation site on ETF-β. 34127633 8 69 part_of site 1355:1358 arg1 ETF-β ETF-β site PUBTATOR Site ETF-β 2108 site Western blotting determined the protein abundance of electron transfer flavoprotein (ETF) subunits, including inhibitory methylation site on ETF-β. 32878894 9 109 part_of ALV-J 2291:2295 arg1 the C terminus ALV-J gp85 the C terminus Cterm Site ALV-J gp85 terminus Our results showed that the amino acids 38 to 131 of the N terminus and 159 to 283 of the C terminus of ALV-J gp85 controlled the efficiency of gp85 binding to chNHE1 and were critical for viral infection. 34490144 9 31 gly non-glycosylated 1583:1598 arg1 the non-glycosylated variant the non-glycosylated variant Fterm variant We can demonstrate by ELISA that serum from patients enrolled in an ETEC H10407 controlled infection study are significantly more reactive with glycosylated YghJ compared to the non-glycosylated variant. 34490144 9 67 gly glycosylated 1549:1560 arg1 glycosylated YghJ glycosylated YghJ PUBTATOR YghJ 2847716 We can demonstrate by ELISA that serum from patients enrolled in an ETEC H10407 controlled infection study are significantly more reactive with glycosylated YghJ compared to the non-glycosylated variant. 32922663 8 54 gly glycosylation 1486:1498 arg1 ECM related proteins ECM related proteins Fterm proteins In concordance with the previously published bladder cancer cell line N-glycoproteomic data, we also observed dysregulated glycosylation in ECM related proteins. 34132588 5 7 gly glycoprotein 625:636 arg1 a glycoprotein a glycoprotein Fterm glycoprotein We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion of aglB blocked S-layer glycosylation and abolished surface-associated growth. 34132588 5 7 gly glycoprotein 625:636 arg1 strain S2 strain S2 Cterm S2 We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion of aglB blocked S-layer glycosylation and abolished surface-associated growth. 33886622 2 40 gly N-glycosylated 315:328 arg1 COX COX asparagine residues OGER COX asparagine residues In vertebrates and corals, COX must be N-glycosylated on at least two asparagine residues in the N-(X)-S/T motif to be catalytically active. 33886622 2 40 gly N-glycosylated 315:328 arg2 at least two asparagine residues asparagine residues asparagine residues In vertebrates and corals, COX must be N-glycosylated on at least two asparagine residues in the N-(X)-S/T motif to be catalytically active. 33886622 2 40 gly N-glycosylated 315:328 arg2 at least two asparagine residues COX asparagine residues OGER COX asparagine residues In vertebrates and corals, COX must be N-glycosylated on at least two asparagine residues in the N-(X)-S/T motif to be catalytically active. 33370382 1 58 part_of contains 205:212 arg1 a precursor gp160 AND an ER-targeting signal peptide a precursor gp160 an ER-targeting signal peptide PUBTATOR Site gp160 2028 peptide HIV-1 envelope (Env) is a trimer of gp120-gp41 heterodimers, synthesized from a precursor gp160 that contains an ER-targeting signal peptide (SP) at its amino-terminus. 34734627 4 14 gly glycosylation 630:642 arg1 hemopexin hemopexin PUBTATOR hemopexin 3263 We found that the efficiency of glycosylation of hemopexin was dependent upon a robust NADPH-dependent cytosolic reductive pathway, which could be mimicked by the addition of a membrane-impermeable reducing agent. 33578036 4 34 gly glycosylation 846:858 arg1 asparagine residues asparagine residues asparagine residues Using this bioreporter, we uncovered critical host and viral determinants of the interaction, including a role for glycosylation of asparagine residues within the RBD in mediating successful viral entry. 34520755 3 54 gly N-glycosylation 667:681 arg2 the potential N-glycosylation sites the potential N-glycosylation sites sites Removal of the N-linked glycans from rHuPH20 with PNGase F shifted the molecular weight from 66 kDa to approximately 52 kDa, its deduced molecular weight based on sequence analysis, suggesting that most, if not all, of the potential N-glycosylation sites are linked to oligosaccharides. 33171989 4 5 part_of ECM 533:535 arg1 the ECM composition ECM the ECM composition OGER Site ECM Q13201 position However, neither the ECM composition of plant-associated pseudomonads nor their phylogenetic distribution within the genus has been so thoroughly studied. 32296775 1 44 part_of containing 211:220 arg1 two designed amphiphilic peptides AND short hydrophobic phenylalanine (F) and cationic arginine (R) sequences two designed amphiphilic peptides short hydrophobic phenylalanine (F) and cationic arginine (R) sequences sequences The self-assembly behavior and antimicrobial activity of two designed amphiphilic peptides, R3F3 and R4F4, containing short hydrophobic phenylalanine (F) and cationic arginine (R) sequences, are investigated. 32709339 6 114 gly fragment 863:870 arg1 The parent and sugar fragment ions The parent and sugar fragment ions The parent and sugar fragment ions of other saponins were predicted using the above mentioned fragmentation pattern. 34725712 2 49 part_of TPR 356:358 arg1 the TPR domain TPR the TPR domain OGER Site TPR P12270 domain OGT and its isoforms select from these substrates through the tetratricopeptide repeat (TPR) domain, yet the impact of truncations to the TPR domain on substrate and glycosite selection is unresolved. 33578036 3 5 part_of protein 688:694 arg1 the SARS-CoV-2 S protein and ACE2 ectodomain S protein the SARS-CoV-2 S protein and ACE2 ectodomain OGER Site S protein Q15517 ectodomain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 33578036 3 5 part_of protein 688:694 arg1 the S receptor-binding domain S protein the S receptor-binding domain OGER Site S protein Q15517 domain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 33578036 3 35 part_of receptor-binding 638:653 arg1 the SARS-CoV-2 S protein and ACE2 ectodomain receptor the SARS-CoV-2 S protein and ACE2 ectodomain Fterm Site receptor ectodomain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 33578036 3 35 part_of receptor-binding 638:653 arg1 the S receptor-binding domain receptor the S receptor-binding domain Fterm Site receptor domain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 33578036 3 46 part_of S 636:636 arg1 the SARS-CoV-2 S protein and ACE2 ectodomain S the SARS-CoV-2 S protein and ACE2 ectodomain PUBTATOR Site S 43740568 ectodomain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 33578036 3 46 part_of S 636:636 arg1 the S receptor-binding domain S the S receptor-binding domain PUBTATOR Site S 43740568 domain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 33578036 3 53 part_of ACE2 700:703 arg1 the SARS-CoV-2 S protein and ACE2 ectodomain ACE2 the SARS-CoV-2 S protein and ACE2 ectodomain PUBTATOR Site ACE2 59272 ectodomain The bioreporter assay is based on a nanoluciferase complementation reporter, composed of two subunits, large BiT and small BiT, fused to the S receptor-binding domain (RBD) of the SARS-CoV-2 S protein and ACE2 ectodomain, respectively. 34622651 7 47 gly glycopeptide 1006:1017 arg2 intact glycopeptide quantification intact glycopeptide quantification glycopeptide The established analysis platform utilizes a well-designed DIA-MS method for raw data collection, a spectral library constructed specifically for intact glycopeptide quantification providing accurate results by the inclusion of Y ions for quantification and filtering of quantified intact glycopeptides with low-quality MS2 spectra automatically using a set of criteria. 34622651 7 86 gly glycopeptides 1142:1154 arg2 quantified intact glycopeptides quantified intact glycopeptides glycopeptides The established analysis platform utilizes a well-designed DIA-MS method for raw data collection, a spectral library constructed specifically for intact glycopeptide quantification providing accurate results by the inclusion of Y ions for quantification and filtering of quantified intact glycopeptides with low-quality MS2 spectra automatically using a set of criteria. 31859482 0 3 part_of Glycoproteins 65:77 arg1 Glycosite Glycoproteins Glycosite Fterm Site Glycoproteins Glycosite Glycans, Glycosite, and Intact Glycopeptide Analysis of N-Linked Glycoproteins Using Liquid Handling Systems. 32401558 2 15 gly glycoproteins 488:500 arg1 carbohydrate-rich molecules glycoproteins carbohydrate-rich molecules Fterm glycoproteins Confocal laser scanning microscopy revealed a dense mycelial mass, with an ECM covering/interspersing the fungal cells and containing carbohydrate-rich molecules (e.g. glycoproteins) and extracellular DNA. 32987930 3 34 gly N-glycosylation 468:482 arg1 viral envelope proteins viral envelope proteins Fterm proteins N-glycosylation of viral envelope proteins may be crucial for viral virulence and a possible target for its purposed attenuation. 32892942 5 77 gly glycopeptides 714:726 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides The enrichment efficiency of PAM-OH HMSs was demonstrated by capturing N-linked glycopeptides from tryptic digest of human immunoglobulin G (IgG). 33272761 4 12 gly fucosylated 432:442 arg1 a fucosylated glycan structural feature a fucosylated glycan structural feature Patatin had a fucosylated glycan structural feature, which strongly bound AAL (Aleuria aurantia Leukoagglutinin), a known fucose binding lectin. 33272761 4 42 gly had 426:428 arg1 Patatin AND a fucosylated glycan structural feature Patatin a fucosylated glycan structural feature PUBTATOR Patatin 102577633 Patatin had a fucosylated glycan structural feature, which strongly bound AAL (Aleuria aurantia Leukoagglutinin), a known fucose binding lectin. 33409271 11 101 part_of enzyme 1598:1603 arg1 the peptidase domain angiotensin-converting enzyme 2 the peptidase domain PUBTATOR Site angiotensin-converting enzyme 2 59272 domain Complex formation with the peptidase domain of human angiotensin-converting enzyme 2 (ACE2), the receptor for the 2019-nCoV spike RBD, was confirmed by SEC, both for the YFP-fused spike RBD and for spike RBD alone, after removal of YFP by proteolytic cleavage. 33889548 5 60 gly glycosylation 909:921 arg1 serum PON1 serum PON1 PUBTATOR PON1 5444 Quantitative lens culinaris agglutin (LCA) ELISA was further used to confirm the difference of glycosylation on serum PON1 in liver diseases (n = 56). 32393178 4 97 part_of sequence 847:854 arg1 HA HA sequence Cterm Site HA None sequence RESULTS A multi-task learning sparse group least absolute shrinkage and selection operator (LASSO) (MTL-SGL) regression method was developed and applied to derive two types of predominant features including protein sequence and N-linked glycosylation in hemagglutinin (HA) affecting variations in serologic data for human and swine H1N1 IAVs. 32393178 4 97 part_of sequence 847:854 arg1 hemagglutinin hemagglutinin sequence Fterm Site hemagglutinin None sequence RESULTS A multi-task learning sparse group least absolute shrinkage and selection operator (LASSO) (MTL-SGL) regression method was developed and applied to derive two types of predominant features including protein sequence and N-linked glycosylation in hemagglutinin (HA) affecting variations in serologic data for human and swine H1N1 IAVs. 33788965 12 45 gly N-glycosylation 1740:1754 arg1 CALHM1/3 subunits CALHM1/3 subunits PUBTATOR CALHM1/3 subunits 255022 Overall, this study characterized N-glycosylation and S-palmitoylation of CALHM1/3 subunits and clarified their differential contributions to its functional expression, providing insights into the fine control of the CALHM1/3 channel and associated physiological processes. 34863021 1 74 gly glycoprotein 219:230 arg1 glycoprotein factor VIII glycoprotein factor VIII Fterm glycoprotein BACKGROUND A portion of individuals with hemophilia A develop neutralizing antibodies called inhibitors to glycoprotein factor VIII (FVIII). 34335620 9 0 part_of regions 1973:1979 arg1 the GP GP regions PUBTATOR Site GP 55819 regions Molecular and functional analysis of proteomics-identified mAbs revealed recognition of epitopes in three major antigenic sites - the GP head domain, the glycan cap, and the base region, with a high prevalence of neutralizing and protective mAb specificities that targeted the base and glycan cap regions on the GP. 34335620 9 2 part_of GP 1810:1811 arg1 three major antigenic sites GP three major antigenic sites PUBTATOR Site GP 55819 sites Molecular and functional analysis of proteomics-identified mAbs revealed recognition of epitopes in three major antigenic sites - the GP head domain, the glycan cap, and the base region, with a high prevalence of neutralizing and protective mAb specificities that targeted the base and glycan cap regions on the GP. 34335620 9 2 part_of GP 1810:1811 arg1 the GP head domain GP the GP head domain PUBTATOR Site GP 55819 domain Molecular and functional analysis of proteomics-identified mAbs revealed recognition of epitopes in three major antigenic sites - the GP head domain, the glycan cap, and the base region, with a high prevalence of neutralizing and protective mAb specificities that targeted the base and glycan cap regions on the GP. 31919391 5 62 gly N-glycopeptides 1250:1264 arg2 N-glycopeptides N-glycopeptides N-glycopeptides This study provides a feasible strategy for the surface functionalized novel materials for isolation and enrichment of N-glycopeptides. 32501708 7 45 gly glycopeptides 862:874 arg1 three GalNAc localization variants glycopeptides three GalNAc localization variants glycopeptides Here, we apply it to representative mucin glycopeptides with O-linked glycans: three GalNAc localization variants, a pair with α/β GalNAc anomers, and another with GalNAc/GlcNAc isomers. 32501708 7 45 gly glycopeptides 862:874 arg1 O-linked glycans glycopeptides O-linked glycans glycopeptides Here, we apply it to representative mucin glycopeptides with O-linked glycans: three GalNAc localization variants, a pair with α/β GalNAc anomers, and another with GalNAc/GlcNAc isomers. 32846088 6 36 gly SARS-CoV-2 759:768 arg1 The O-linked glycan domain SARS The O-linked glycan domain OGER SARS P49591 The O-linked glycan domain of SARS-CoV-2 is highly positively charged, which may promote binding to negatively charged human cells. 32120884 3 42 part_of mucin-like 805:814 arg1 mucin-like domains mucin mucin-like domains PUBTATOR Site mucin 100508689 domains Jingmen flavi-like virus glycoproteins have a sequence enriched in serine, threonine, and proline at the amino terminus, which is a feature of mucin-like domains. 32120884 3 65 part_of have 701:704 arg1 Jingmen flavi-like virus glycoproteins AND a sequence Jingmen flavi-like virus glycoproteins a sequence Fterm Site glycoproteins sequence Jingmen flavi-like virus glycoproteins have a sequence enriched in serine, threonine, and proline at the amino terminus, which is a feature of mucin-like domains. 33335689 1 59 gly used 174:177 arg2 The hinge region The hinge region region The hinge region of immunoglobulin G1 (IgG1) is used as a common linker for Fc-fusion therapeutic proteins. 32102257 3 16 gly glycosylation 627:639 arg2 N-linked glycosylation sites N-linked glycosylation sites sites Here, we focused on specific immune-escape mutations associated with the acquisition of N-linked glycosylation sites in HBsAg (NLGSs). 34386963 3 4 part_of precursor 618:626 arg1 lipopeptides precursor lipopeptides Fterm Site precursor lipopeptides A generalized procedure for the synthesis of S-lipopeptides is described which facilities rapid preparation of tens of analogs of lipopeptides from a single thiolated polypeptide precursor. 34811775 7 104 gly glycosylation 1351:1363 arg2 additional N-linked glycosylation site additional N-linked glycosylation site site Presence of additional N-linked glycosylation site was noted in the G12 strains, as a consequence of a change from Asp→Asn at amino acid position 238. 32393178 2 56 gly glycosylation 432:444 arg1 influenza glycoproteins influenza glycoproteins Fterm glycoproteins Antigenic property of influenza viruses are determined by both protein sequence and N-linked glycosylation of influenza glycoproteins, especially hemagglutinin (HA). 32393178 2 107 gly glycoproteins 459:471 arg1 influenza glycoproteins influenza glycoproteins Fterm glycoproteins Antigenic property of influenza viruses are determined by both protein sequence and N-linked glycosylation of influenza glycoproteins, especially hemagglutinin (HA). 34646892 7 17 gly c-Myc 1086:1090 arg1 O-GlcNAcylation c-Myc O-GlcNAcylation PUBTATOR c-Myc 4609 O-GlcNAcylation of c-Myc was confirmed using immunoprecipitation and proximity ligation assay. 32012886 3 29 gly sialoglycoforms 469:483 arg1 PrPC sialoglycoforms PrPC sialoglycoforms PUBTATOR PrPC 19122 Prion strains replicate by selecting substrates from a large pool of PrPC sialoglycoforms expressed by a host. 33216553 5 57 gly sialylated 889:898 arg1 977 formerly sialylated N-linked glycoproteins 977 formerly sialylated N-linked glycoproteins Fterm glycoproteins A total of 8313 protein groups were identified, including 7827 protein groups from nonmodified peptides, 3803 phosphoproteins, and 977 formerly sialylated N-linked glycoproteins. 33216553 5 60 gly glycoproteins 909:921 arg1 977 formerly sialylated N-linked glycoproteins 977 formerly sialylated N-linked glycoproteins Fterm glycoproteins A total of 8313 protein groups were identified, including 7827 protein groups from nonmodified peptides, 3803 phosphoproteins, and 977 formerly sialylated N-linked glycoproteins. 35499042 16 42 gly SP1 2299:2301 arg1 O-GlcNAcylation SP1 O-GlcNAcylation OGER SP1 P08047 Furthermore, the transcription factor SP1 specifically bound to the AQP3 promoter region, and O-GlcNAcylation of SP1 increased its stability and transcriptional regulation of AQP3 which is associated with O-GlcNAcylation of SP1. 35499042 16 53 gly SP1 2188:2190 arg1 O-GlcNAcylation SP1 O-GlcNAcylation OGER SP1 P08047 Furthermore, the transcription factor SP1 specifically bound to the AQP3 promoter region, and O-GlcNAcylation of SP1 increased its stability and transcriptional regulation of AQP3 which is associated with O-GlcNAcylation of SP1. 32501708 0 0 gly Peptide 87:93 arg1 the Peptide and Glycan Levels the Peptide and Glycan Levels High-Resolution Ion Mobility Separations of Isomeric Glycoforms with Variations on the Peptide and Glycan Levels. 33557755 2 96 gly glycosylation 327:339 arg2 N-linked glycosylation sites N-linked glycosylation sites sites This variability, as well as increasing incorporation of N-linked glycosylation sites, is fundamental to this evasion. 34622651 6 24 gly glycopeptides 808:820 arg2 intact glycopeptides intact glycopeptides glycopeptides In this study, we have established a novel integrated platform for the DIA analysis of intact glycopeptides isolated from complex samples. 33933451 6 20 gly N-glycosylated 905:918 arg1 WT PMP22 WT PMP22 PUBTATOR WT PMP22 Q01453 Depletion of oligosaccharyltransferase OST-A and OST-B subunits revealed that WT PMP22 is N-glycosylated posttranslationally by OST-B, whereas L16P is cotranslationally glycosylated by OST-A. 32498907 0 10 gly glycopeptides 75:87 arg2 N-linked glycopeptides N-linked glycopeptides glycopeptides Glutathione-modified ordered mesoporous silicas for enrichment of N-linked glycopeptides by hydrophilic interaction chromatography. 33888690 0 47 gly glycoprotein 20:31 arg1 cell-free glycoprotein synthesis cell-free glycoprotein synthesis Fterm glycoprotein Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles. 32012886 5 10 gly PrPSc 755:759 arg1 sialylation PrPSc sialylation PUBTATOR PrPSc 19122 Toward addressing this question, the current study looked into a possibility that sialylation of PrPSc might be involved in defining selective vulnerability of brain regions. 32012886 5 26 gly sialylation 740:750 arg1 PrPSc PrPSc PUBTATOR PrPSc 19122 Toward addressing this question, the current study looked into a possibility that sialylation of PrPSc might be involved in defining selective vulnerability of brain regions. 33451024 2 71 gly N-glycosylation 423:437 arg2 N-glycosylation sites N-glycosylation sites sites We previously reported that DC-SIGN mediated H5N1 influenza A virus (AIVs) infection, however, the important DC-SIGN interaction with N-glycosylation sites remain unknown. 34644163 8 7 gly N-glycosylation 1164:1178 arg1 GFP-CTD recombinant proteins GFP-CTD recombinant proteins Fterm proteins CTD N-glycosylation is important for the secretion and localization of GFP-CTD recombinant proteins in C. hutchinsonii. 32221039 9 104 part_of Fab-N-linked-glycosylation 1682:1707 arg1 Fab-N-linked-glycosylation introduction sites Fab Fab-N-linked-glycosylation introduction sites PUBTATOR Site Fab 2187 sites Fab-N-linked-glycosylation introduction sites are observed in a minority of anti-NET B cell clones but can strongly influence NET-Ag binding. 33154460 3 25 gly glycosylated 540:551 arg1 Gc2 Gc2 PUBTATOR Gc2 83733 In contrast, Gc2 is not glycosylated. 34455356 6 40 part_of NS1 1265:1267 arg1 the glycosylation sites NS1 the glycosylation sites OGER Site NS1 Q9Y6Y0 sites The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 34455356 6 40 part_of NS1 1265:1267 arg1 N175 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 34668190 9 52 gly p65 1671:1673 arg1 significantly increased glucose metabolism p65 significantly increased glucose metabolism PUBTATOR p65 19697 In addition, increased O-GlcNAcylation was associated with significantly increased glucose metabolism, and OGT-mediated O-GlcNAcylation of p65. 34668190 9 52 gly p65 1671:1673 arg1 OGT-mediated O-GlcNAcylation p65 OGT-mediated O-GlcNAcylation PUBTATOR p65 19697 In addition, increased O-GlcNAcylation was associated with significantly increased glucose metabolism, and OGT-mediated O-GlcNAcylation of p65. 33392644 9 18 gly protein 1760:1766 arg1 glycan milieu protein glycan milieu Fterm protein These findings suggest that N-glycosylation disruption rescues the NaV1.5 channel possibly through the alteration of ubiquitin-proteasome activity, and changes gating properties of the NaV1.5 channel by modulating glycan milieu of the channel protein. 34622651 9 34 gly glycopeptides 1482:1494 arg2 1123 nonredundant intact glycopeptides 1123 nonredundant intact glycopeptides glycopeptides By utilizing 100 isolation windows for DIA data acquisition, a well-constructed human serum spectral library containing 1123 nonredundant intact glycopeptides with Y ions, and automated data inspection, 620 intact glycopeptides were quantified with high confidence from DIA-MS. 34622651 9 71 gly glycopeptides 1551:1563 arg2 620 intact glycopeptides 620 intact glycopeptides glycopeptides By utilizing 100 isolation windows for DIA data acquisition, a well-constructed human serum spectral library containing 1123 nonredundant intact glycopeptides with Y ions, and automated data inspection, 620 intact glycopeptides were quantified with high confidence from DIA-MS. 32123732 6 7 gly glycoproteins 1282:1294 arg1 defined glycoproteins defined glycoproteins Fterm glycoproteins This strategy to site-specifically control the installation of N-linked monosaccharides for elaboration to a variety of functional N-glycans overcomes a major limitation in synthesizing defined glycoproteins for research and therapeutic applications. 34734627 6 64 gly glycosylation 950:962 arg2 this site site site We show that efficient glycosylation at this site is influenced by the cytosolic reductive pathway acting on both STT3A- and STT3B-dependent glycosylation. 34625256 2 18 gly N-glycosylation 413:427 arg1 PSA PSA OGER PSA P07288 The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. 34625256 2 18 gly N-glycosylation 413:427 arg1 prostate-specific antigen prostate-specific antigen PUBTATOR prostate-specific antigen 354 The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. 35386843 6 9 gly glycopeptides 1003:1015 arg2 intact glycopeptides intact glycopeptides glycopeptides This work also showed that DPS-MS is applicable for direct detection of intact glycopeptides and nucleotide sugars as well as determination of glycosylation profiling of antibody, such as NIST monoclonal antibody IgG (NISTmAb). 33476498 2 57 part_of factors 313:319 arg1 the composition factors the composition Fterm Site factors position However, the influence of the genetic affiliation of the culture and the technological factors of yeast strains, the composition of growth media on the efficiency of essential trace elements incorporation into the biomass and on the change of theirs intracellular components content have hardly been investigated. 32331895 0 55 gly N-glycosylation 43:57 arg1 bovine peptide transporter 2 bovine peptide transporter 2 PUBTATOR peptide transporter 2 Q16348 Short communication: The essential role of N-glycosylation in the transport activity of bovine peptide transporter 2. 32822926 8 67 gly composition 1509:1519 arg1 the most important determinant position the most important determinant position The sedimentary matrix is the most important determinant of the composition of the biofilm and the occurrence of EDCs. 32065175 3 46 gly glycopeptide 688:699 arg2 glycopeptide glycopeptide glycopeptide In this work we adapted a software tool, MS-Filter, to search HCD peak list files for predicted Y ions based on matched EThcD results to propose additional glycopeptide assignments. 33788965 11 22 gly CALHM3 1591:1596 arg1 a critical determinant CALHM3 a critical determinant PUBTATOR CALHM3 119395 However, the same mutation markedly attenuated the channel activity but not surface localization of CALHM1/3, suggesting that CALHM3 palmitoylation is a critical determinant of CALHM1/3 activity but not its formation or forward trafficking. 34227365 9 168 gly N-glycoproteins 1915:1929 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins We found that N-glycoproteins and N-glycopeptides were highly enriched in a trifluoroacetic acid system with 5-μm filling particles in the HILIC column. 34227365 9 172 gly N-glycopeptides 1935:1949 arg2 N-glycopeptides N-glycopeptides N-glycopeptides We found that N-glycoproteins and N-glycopeptides were highly enriched in a trifluoroacetic acid system with 5-μm filling particles in the HILIC column. 33276951 2 36 gly glycosylation 321:333 arg1 SARS-CoV-2-NP SARS-CoV-2-NP OGER SARS P49591 We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. 33667901 1 36 part_of proteins 381:388 arg1 serine proteins serine and threonine residues Fterm AminoAcid proteins serine and threonine residues Protein O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation), an essential post-translational as well as cotranslational modification, is the attachment of β-D-N-acetylglucosamine to serine and threonine residues of nucleocytoplasmic proteins. 32443590 5 60 gly glycoprotein 1134:1145 arg1 hydrolyzed high-mannose-containing glycoprotein hydrolyzed high-mannose-containing glycoprotein The supplement of hydrolyzed high-mannose-containing glycoprotein by GH92 and EndoSS could completely restore the growth deficiency of endoSS deletion mutant in swine serum. 34455356 2 27 gly glycosylation 369:381 arg2 three potential predicted N-linked glycosylation sites three potential predicted N-linked glycosylation sites sites DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and 207. 34455356 2 44 gly residues 392:399 arg1 207 residues 130, 175 and 207 residues 130, 175 and 207 DTMUV nonstructural protein 1 (NS1) contains three potential predicted N-linked glycosylation sites at residues 130, 175 and 207. 34490144 7 81 gly glycosylation 1044:1056 arg2 The glycosylation sites The glycosylation sites sites The glycosylation sites are evenly distributed throughout the sequence and do not appear to affect the folding of the overall protein structure. 33024038 5 113 gly glycoproteins 828:840 arg1 CD55 CD55 OGER CD55 P08174 In this work, we show that CpaA cleaves a broader range of O-linked human glycoproteins, including several glycoproteins involved in complement activation, such as CD55 and CD46. 33024038 5 113 gly glycoproteins 828:840 arg1 CD46 CD46 OGER CD46 P15529 In this work, we show that CpaA cleaves a broader range of O-linked human glycoproteins, including several glycoproteins involved in complement activation, such as CD55 and CD46. 33024038 5 113 gly glycoproteins 828:840 arg1 several glycoproteins several glycoproteins Fterm glycoproteins In this work, we show that CpaA cleaves a broader range of O-linked human glycoproteins, including several glycoproteins involved in complement activation, such as CD55 and CD46. 33024038 5 119 gly glycoproteins 795:807 arg1 several glycoproteins glycoproteins Fterm glycoproteins In this work, we show that CpaA cleaves a broader range of O-linked human glycoproteins, including several glycoproteins involved in complement activation, such as CD55 and CD46. 33877808 1 27 gly glycopeptides 283:295 arg2 intact glycopeptides intact glycopeptides glycopeptides The heterogeneity and low abundance of protein glycosylation present challenging barriers to the analysis of intact glycopeptides, which is key to comprehensively understanding the role of glycosylation in an organism. 35499042 1 69 part_of threonine 274:282 arg1 nuclear, cytoplasmic, and mitochondrial proteins proteins threonine Fterm AminoAcid proteins 360 threonine residues Introduction O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification is a post-translational modification in which a single O-GlcNAc is added to serine or threonine residues in nuclear, cytoplasmic, and mitochondrial proteins, and is involved in a variety of physiological processes. 35499042 1 73 part_of serine 264:269 arg1 nuclear, cytoplasmic, and mitochondrial proteins proteins serine Fterm AminoAcid proteins 360 serine Introduction O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification is a post-translational modification in which a single O-GlcNAc is added to serine or threonine residues in nuclear, cytoplasmic, and mitochondrial proteins, and is involved in a variety of physiological processes. 33900328 5 18 gly glycoproteins 760:772 arg1 glycoproteins glycoproteins Fterm glycoproteins Four proteins, including FIP-glu (WT) and its mutants N31S, T36N and N31S/T36N, were successfully expressed in P. pastoris, of which T36N and N31S/T36N were glycoproteins. 31859482 7 61 gly deglycosylated 1169:1182 arg1 deglycosylated peptides deglycosylated peptides peptides Furthermore, our approach enabled the analysis of deglycosylated peptides and glycans from enriched IGPs following PNGase F digest. 31859482 7 63 gly peptides 1184:1191 arg1 glycans peptides glycans peptides Furthermore, our approach enabled the analysis of deglycosylated peptides and glycans from enriched IGPs following PNGase F digest. 34004049 1 7 gly proteins 173:180 arg1 O-GlcNAc proteins O-GlcNAc Fterm proteins O-GlcNAc is a common post-translational modification of nuclear, mitochondrial, and cytoplasmic proteins that regulates normal physiology and the cell stress response. 32878894 10 122 part_of gp85 2472:2475 arg1 N11 gp85 N11 Cterm SpecificSite gp85 N11 In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32878894 10 122 part_of gp85 2472:2475 arg1 the glycosylation sites gp85 the glycosylation sites Cterm Site gp85 sites In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32878894 10 122 part_of gp85 2472:2475 arg1 cysteines gp85 cysteines Cterm AminoAcid gp85 cysteines In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32393178 4 14 gly glycosylation 869:881 arg1 HA HA Cterm HA None RESULTS A multi-task learning sparse group least absolute shrinkage and selection operator (LASSO) (MTL-SGL) regression method was developed and applied to derive two types of predominant features including protein sequence and N-linked glycosylation in hemagglutinin (HA) affecting variations in serologic data for human and swine H1N1 IAVs. 32393178 4 14 gly glycosylation 869:881 arg1 hemagglutinin hemagglutinin Fterm hemagglutinin None RESULTS A multi-task learning sparse group least absolute shrinkage and selection operator (LASSO) (MTL-SGL) regression method was developed and applied to derive two types of predominant features including protein sequence and N-linked glycosylation in hemagglutinin (HA) affecting variations in serologic data for human and swine H1N1 IAVs. 33270417 8 41 gly glycoprotein 1245:1256 arg1 A glycoprotein A glycoprotein Fterm glycoprotein A glycoprotein, RNase B, also showed a significantly elongated deamidation half-life compared to nonglycosylated protein RNase A. 33270417 8 41 gly glycoprotein 1245:1256 arg1 RNase B RNase B OGER RNase B P07998 A glycoprotein, RNase B, also showed a significantly elongated deamidation half-life compared to nonglycosylated protein RNase A. 34215068 6 23 gly glycopeptides 1210:1222 arg2 glycopeptides glycopeptides glycopeptides This affinity tip established good and tunable permeability and was used to selectively enrich glycopeptides as well as phosphopeptides. 34227365 16 187 gly N-glycopeptides 3005:3019 arg2 the physiologically abundant urinary N-glycopeptides the physiologically abundant urinary N-glycopeptides N-glycopeptides A label-free quantitation strategy was used to investigate the fluctuation range of the physiologically abundant urinary N-glycopeptides. 33552834 2 25 gly glycosylated 403:414 arg1 the glycosylated metalloprotease domain the glycosylated metalloprotease domain domain The spike 2 protein recognizes the N-terminal helices of the glycosylated metalloprotease domain in the human ACE2 receptor. 34342946 3 39 part_of butyrophilin 496:507 arg1 The butyrophilin ectodomain butyrophilin The butyrophilin ectodomain Fterm Site butyrophilin ectodomain The butyrophilin ectodomain exhibits two subdomains with immunoglobulin fold, each comprising a β-sandwich with a central disulfide bridge as well as one N-linked glycosylation. 32393178 5 24 gly glycosylation 1032:1044 arg2 N-linked glycosylation sites N-linked glycosylation sites sites Results suggested that mutations and changes in N-linked glycosylation sites are associated with the rise of antigenic variants of H1N1 IAVs. 33242079 10 105 gly α2,6-sialylated 1616:1630 arg1 a few α2,6-sialylated N-glycans a few α2,6-sialylated N-glycans Moreover, it was revealed that a few α2,6-sialylated N-glycans were produced by the action of St6gal1 gene, despite both St6gal1 and St6gal2 genes being expressed in the adult mouse brain. 33906120 0 52 gly glycosylated 48:59 arg1 central glycosylated proteins central glycosylated proteins Fterm proteins N-linked glycoproteome analysis reveals central glycosylated proteins involved in wheat early seedling growth. 33578036 0 11 gly glycosylation 84:96 arg1 SARS-CoV-2 S SARS-CoV-2 S PUBTATOR S 43740568 Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry. 32633815 10 18 part_of has 1314:1316 arg1 SARS-CoV-2 AND a polybasic cleavage site SARS-CoV-2 a polybasic cleavage site OGER Site SARS P49591 site SARS-CoV-2 has a polybasic cleavage site with an inserted sequence of PRRA compared to RaTG13 and only PRR to pangolin. 32188979 7 68 gly Hyl-O-glycosylation 1506:1524 arg1 proteins proteins Fterm proteins The adapted force field parameters for O-linkages between Hyl and carbohydrates will aid future computational studies on proteins with Hyl-O-glycosylation. 32331895 5 35 part_of bPepT2 907:912 arg1 AA composition bPepT2 AA composition Cterm Site bPepT2 Q16348 position BioXM (http://202.195.246.60/BioXM/) and TMHMM (http://www.cbs.dtu.dk/services/TMHMM-2.0/) software were used to predict the AA composition and transmembrane domain of bPepT2, respectively. 32331895 5 35 part_of bPepT2 907:912 arg1 transmembrane domain bPepT2 transmembrane domain Cterm Site bPepT2 Q16348 domain BioXM (http://202.195.246.60/BioXM/) and TMHMM (http://www.cbs.dtu.dk/services/TMHMM-2.0/) software were used to predict the AA composition and transmembrane domain of bPepT2, respectively. 32439286 4 20 gly proteins 505:512 arg1 glycans proteins glycans Fterm proteins Isolating glycans from proteins and tagging a label on glycans is the most commonly used technique for glycan profiling. 33276951 5 31 gly co-located 690:699 arg1 the putative N-linked glycosylation sites the putative N-linked glycosylation sites sites The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes. 33276951 5 35 gly glycosylation 546:558 arg1 this protein this protein Fterm protein 43740575 The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes. 33276951 5 38 gly glycosylation 629:641 arg2 the putative N-linked glycosylation sites the putative N-linked glycosylation sites sites The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes. 33276951 5 38 gly glycosylation 629:641 arg2 the amino acid positions the amino acid positions positions 48 and 270, The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes. 33276951 5 30 gly positions 668:676 arg1 270 positions 48 and 270, positions 48 and 270, The glycosylation pattern analysis of this protein revealed that the putative N-linked glycosylation sites, at the amino acid positions 48 and 270, co-located with two of the main immunodominant B cell epitopes. 32439055 3 55 gly glycoprotein 513:524 arg1 glycoprotein glycoprotein Fterm glycoprotein As the molar ratio of the mixture of tryptic digests of HRP, glycoprotein (HRP), and nonglycoprotein (BSA) reached 1:800:800, good selectivity of the Ti-MOF nanosheets were still clearly observed. 32439055 3 58 gly nonglycoprotein 537:551 arg1 nonglycoprotein nonglycoprotein Fterm nonglycoprotein As the molar ratio of the mixture of tryptic digests of HRP, glycoprotein (HRP), and nonglycoprotein (BSA) reached 1:800:800, good selectivity of the Ti-MOF nanosheets were still clearly observed. 33997889 10 49 part_of ComP 1612:1615 arg1 a ComP fragment ComP a ComP fragment OGER Site ComP P49747 fragment Moreover, we identify novel sites on the surface of EPA that are amenable to ComP sequon insertion and find that Cross-Reactive Material 197 fused to a ComP fragment is also glycosylated. 33668618 1 48 part_of have 217:220 arg1 fPS-2 AND elongated heads fPS heads OGER Site fPS P14324 heads The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. 33668618 1 48 part_of have 217:220 arg1 fPS-65 AND elongated heads fPS heads OGER Site fPS P14324 heads The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. 33668618 1 48 part_of have 217:220 arg1 fPS-2 AND long contractile tails fPS tails OGER Site fPS P14324 tails The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. 33668618 1 48 part_of have 217:220 arg1 fPS-65 AND long contractile tails fPS tails OGER Site fPS P14324 tails The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. 33668618 1 48 part_of have 217:220 arg1 fPS-65 AND elongated heads fPS heads OGER Site fPS P14324 heads The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. 33668618 1 48 part_of have 217:220 arg1 fPS-65 AND long contractile tails fPS tails OGER Site fPS P14324 tails The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. 33171989 5 28 part_of ECM 722:724 arg1 the ECM composition ECM the ECM composition OGER Site ECM Q13201 position In this work, we use in silico methods to describe the ECM composition of Pseudomonas fluorescens F113, a plant growth-promoting rhizobacteria and model for rhizosphere colonization. 33242079 8 4 gly α2,6-sialylated 1368:1382 arg1 α2,6-sialylated glycans α2,6-sialylated glycans Consequently, α2,6-sialylated N-glycans were scarcely detected in adult mouse brain tissues, and a majority of α2,6-sialylated glycans found in the mouse brain were O-linked glycans. 33242079 8 34 gly α2,6-sialylated 1271:1285 arg1 α2,6-sialylated N-glycans α2,6-sialylated N-glycans Consequently, α2,6-sialylated N-glycans were scarcely detected in adult mouse brain tissues, and a majority of α2,6-sialylated glycans found in the mouse brain were O-linked glycans. 33118239 9 62 gly glycoprotein 1319:1330 arg1 glycoprotein synthesis glycoprotein synthesis Fterm glycoprotein These results clearly indicate that this DPDS method is useful for glycoprotein synthesis. 34215068 8 43 gly glycopeptides 1628:1640 arg2 glycopeptides glycopeptides glycopeptides Moreover, this affinity-tip also exhibited high selectivity up to up to 1:1000 (HRP digest to BSA digest) for glycopeptides and 1:200 (β-Casein digest to BSA digest) for phosphopeptides and demonstrated several other fascinating characteristics such as; excellent size exclusion effect for the omission of large-sized proteins, modest backpressure, reproducibility, reusability, smooth enrichment, and successfully applied to a human saliva sample. 34400147 2 74 part_of myocilin 353:360 arg1 the myocilin olfactomedin domain myocilin the myocilin olfactomedin domain OGER Site myocilin domain Despite progress in comprehending the structure and misfolding of the myocilin olfactomedin domain, the structure and function of full-length myocilin, and contextual changes in glaucoma, remain unknown. 33906120 9 24 gly glycosylation 1538:1550 arg2 The glycosylation sites The glycosylation sites sites The glycosylation sites generally occurred on the random coil, which could play roles in maintaining the structural stability of proteins. 32120884 2 14 gly glycoproteins 431:443 arg1 Jingmen flavi-like virus glycoproteins Jingmen flavi-like virus glycoproteins Fterm glycoproteins Computational analyses were used to predict that Jingmen flavi-like virus glycoproteins have structural features of class II viral fusion proteins, including an ectodomain consisting of beta-sheets and short alpha-helices, a fusion peptide with interfacial hydrophobicity and a three-domain architecture. 33886622 9 28 gly glycosylation 1452:1464 arg2 Three potential glycosylation sites COX sites OGER COX sites Three potential glycosylation sites of PmCOX were identified at N79, N170 and N424. 32665399 3 63 gly glycosylated 353:364 arg1 Distinct receptors Distinct receptors Fterm receptors Distinct receptors and effector proteins, many of which are glycosylated, are targeted to each type of granule according to their time of expression, a process called "targeting by timing." 32665399 3 63 gly glycosylated 353:364 arg1 effector proteins effector proteins Fterm proteins Distinct receptors and effector proteins, many of which are glycosylated, are targeted to each type of granule according to their time of expression, a process called "targeting by timing." 35822049 8 2 gly HDAC 1527:1530 arg1 O-GlcNAcylated synaptic proteins HDAC O-GlcNAcylated synaptic proteins OGER HDAC P22760 Using pathway analyses, we found TG-dependent enrichment of O-GlcNAcylated synaptic proteins, trafficking, Notch/Wnt signaling, HDAC signaling, and circadian clock proteins. 33557755 11 16 part_of regions 1856:1862 arg1 HIV sequences regions HIV sequences sequences CONCLUSIONS NGlyAlign is an automated tool for mapping and building glycosylation motif libraries to accurately align highly variable regions in HIV sequences. 32493725 2 118 gly tau 413:415 arg1 O-GlcNAcylation tau O-GlcNAcylation PUBTATOR tau 4137 Tau is subject to O-linked N-acetylglucosamine (O-GlcNAc) modification, and O-GlcNAcylation of tau has been shown to influence tau phosphorylation and aggregation. 34342946 0 60 part_of butyrophilin 40:51 arg1 The extracellular region butyrophilin The extracellular region Fterm Site butyrophilin region The extracellular region of bovine milk butyrophilin exhibits closer structural similarity to human myelin oligodendrocyte glycoprotein than to immunological BTN family receptors. 33607086 8 8 gly glycan 1333:1338 arg1 Asn90 Asn90 Asn90 AminoAcid Asn90 SMD results indicate that SARS-CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 32482891 6 32 part_of ACLP 1134:1137 arg1 a specific N-terminal proteolytic ACLP fragment ACLP a specific N-terminal proteolytic ACLP fragment PUBTATOR Site ACLP 165 fragment Using site-directed mutagenesis, we determined that glycosylation of Asn-471 and Asn-1030 is necessary for ACLP secretion and identified a specific N-terminal proteolytic ACLP fragment. 33454535 0 17 part_of sites 117:121 arg1 ion channels channels sites Fterm Site channels sites Incorporating a transfer learning technique with amino acid embeddings to efficiently predict N-linked glycosylation sites in ion channels. 34566655 7 30 part_of Sp1 857:859 arg1 the E-box1 and Sp1 regions Sp1 the E-box1 and Sp1 regions OGER Site Sp1 P08047 regions Deletion analyses determined that the E-box1 and Sp1 regions play key roles in WGP-induced C4 transcription. 34483834 5 29 gly glycosylation 727:739 arg1 position 7 position 7 position 7 Additionally, we show that glycosylation at position 7 results in a decrease in PKR2 signaling through Gαs without impairing Gαq/ 11 signaling. 34459483 5 28 gly glycoproteins 911:923 arg1 sialylated glycoproteins sialylated glycoproteins Fterm glycoproteins In this study, we identified sialylated glycoproteins in the aortic valve vegetations and plasma of rat and rabbit models of this disease. 34459483 5 44 gly sialylated 900:909 arg1 sialylated glycoproteins sialylated glycoproteins Fterm glycoproteins In this study, we identified sialylated glycoproteins in the aortic valve vegetations and plasma of rat and rabbit models of this disease. 33755116 5 48 gly glycosylated 737:748 arg1 a highly glycosylated protein a highly glycosylated protein Fterm protein We used the HIV-1 Env as a direct applied example of a highly glycosylated protein. 34562451 4 24 gly glycosylation 734:746 arg1 the SP domain the SP domain domain Individual and combinatory site-directed mutagenesis of the glycosylated asparagine residues indicated that glycosylation of the SP domain is critical for TMPRSS13 autoactivation and catalytic activity toward one of its protein substrates, the prostasin zymogen. 34562451 4 39 gly glycosylated 686:697 arg1 the glycosylated asparagine residues the glycosylated asparagine residues asparagine residues Individual and combinatory site-directed mutagenesis of the glycosylated asparagine residues indicated that glycosylation of the SP domain is critical for TMPRSS13 autoactivation and catalytic activity toward one of its protein substrates, the prostasin zymogen. 34725712 3 10 part_of OGT 493:495 arg1 the TPR domain OGT the TPR domain PUBTATOR Site OGT 8473 domain Here, we report the effects of iterative truncations to the TPR domain of OGT on substrate and glycosite selection with the model protein GFP-JunB and the surrounding O-GlcNAc proteome in U2OS cells. 34725712 3 38 part_of TPR 479:481 arg1 the TPR domain TPR the TPR domain OGER Site TPR P12270 domain Here, we report the effects of iterative truncations to the TPR domain of OGT on substrate and glycosite selection with the model protein GFP-JunB and the surrounding O-GlcNAc proteome in U2OS cells. 34069226 0 42 gly Afucosylated 0:11 arg1 Afucosylated IgG Afucosylated IgG Cterm IgG Afucosylated IgG Targets FcγRIV for Enhanced Tumor Therapy in Mice. 32012886 7 0 gly sialylated 985:994 arg1 PrPSc PrPSc PUBTATOR PrPSc 19122 PrPSc in hippocampus and cortex was more sialylated than PrPSc from thalamus and stem. 33863420 7 3 gly glycopeptides 1469:1481 arg2 141 N-linked glycopeptides 141 N-linked glycopeptides glycopeptides From 0.5 mg of tryptic digest of rat brain, 141 N-linked glycopeptides corresponding to 127 glycoproteins and 918 phosphopeptides corresponding to 397 phosphoproteins were enriched simultaneously and identified respectively, proving the Fe3O4@SiO2@(Zr-Ti-MOF)10-NH2 to be a dependable candidate for the simultaneously enrichment of trace phosphopeptides and glycopeptides in intricate biological samples. 33863420 7 28 gly glycoproteins 1504:1516 arg1 127 glycoproteins 127 glycoproteins Fterm glycoproteins From 0.5 mg of tryptic digest of rat brain, 141 N-linked glycopeptides corresponding to 127 glycoproteins and 918 phosphopeptides corresponding to 397 phosphoproteins were enriched simultaneously and identified respectively, proving the Fe3O4@SiO2@(Zr-Ti-MOF)10-NH2 to be a dependable candidate for the simultaneously enrichment of trace phosphopeptides and glycopeptides in intricate biological samples. 33863420 7 44 gly glycopeptides 1770:1782 arg2 glycopeptides glycopeptides phosphopeptides and glycopeptides From 0.5 mg of tryptic digest of rat brain, 141 N-linked glycopeptides corresponding to 127 glycoproteins and 918 phosphopeptides corresponding to 397 phosphoproteins were enriched simultaneously and identified respectively, proving the Fe3O4@SiO2@(Zr-Ti-MOF)10-NH2 to be a dependable candidate for the simultaneously enrichment of trace phosphopeptides and glycopeptides in intricate biological samples. 32443590 0 76 gly Glycoproteins 57:69 arg1 Carbohydrate Source Glycoproteins Carbohydrate Source Fterm Glycoproteins Streptococcus suis Uptakes Carbohydrate Source from Host Glycoproteins by N-glycans Degradation System for Optimal Survival and Full Virulence during Infection. 33545445 1 10 gly Glycans 147:153 arg1 proteins proteins Glycans Fterm proteins Glycans on proteins and cell surfaces are useful biomarkers for determining functional interactions with glycan binding proteins, potential disease states, or indeed level of differentiation. 33877808 4 38 gly O-glycopeptides 989:1003 arg2 O-glycopeptides O-glycopeptides O-glycopeptides The merits of this composite, including high surface area and synergistic effect from size exclusion functionality of mesoporous material, hydrophilic interaction of silica, and the reversible covalent binding with BA, enable the effective and specific enrichment of both intact N- and O-glycopeptides. 34818891 1 12 gly precursors 166:175 arg1 convergent glycan synthesis strategies precursors convergent glycan synthesis strategies Fterm precursors Disaccharide donors are key precursors in convergent glycan synthesis strategies. 33710212 2 36 gly glycosylated 241:252 arg1 mussel adhesive proteins proteins Fterm proteins Adhesive protein-based biomaterials in nature rely on heavily glycosylated proteins such as spider silk and mussel adhesive proteins. 31924761 7 34 gly perilipin 840:848 arg1 O-GlcNAcylation perilipin 1 O-GlcNAcylation PUBTATOR perilipin 1 103968 Loss of OGT decreases O-GlcNAcylation of lipid droplet-associated perilipin 1 (PLIN1), which leads to elevated PLIN1 phosphorylation and enhanced lipolysis. 32174982 10 27 gly AMPK 1362:1365 arg1 O-GlcNAcylation AMPK O-GlcNAcylation PUBTATOR AMPK 5564 We found that O-GlcNAcylation of AMPK suppressed the activity of this regulator, thereby inhibiting ULK1 activity and autophagy. 34677013 15 15 gly used 2394:2397 arg2 two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides peptides Furthermore, two azide-labeled O-GlcNAc (N3-O-GlcNAc) peptides were used to assess whether MSH destroyed the O-GlcNAc modification at the same time. 34056367 9 43 gly glycosylation 1596:1608 arg2 the glycosylation site Asn-Val-Thr site Asn-Val-Thr site Asn-Val-Thr The stepwise method consisted of the glycosylation of a soluble 7-residue peptide, a hydrophobic WALP-NVT peptide, and a γ-sarcoglycan membrane protein, all of which contained the glycosylation site Asn-Val-Thr (NVT). 34056367 9 45 gly glycosylation 1453:1465 arg1 a γ-sarcoglycan membrane protein peptide a γ-sarcoglycan membrane protein peptide The stepwise method consisted of the glycosylation of a soluble 7-residue peptide, a hydrophobic WALP-NVT peptide, and a γ-sarcoglycan membrane protein, all of which contained the glycosylation site Asn-Val-Thr (NVT). 34056367 9 45 gly glycosylation 1453:1465 arg1 a hydrophobic WALP-NVT peptide peptide peptide The stepwise method consisted of the glycosylation of a soluble 7-residue peptide, a hydrophobic WALP-NVT peptide, and a γ-sarcoglycan membrane protein, all of which contained the glycosylation site Asn-Val-Thr (NVT). 34056367 9 45 gly glycosylation 1453:1465 arg1 a hydrophobic WALP-NVT peptide peptide a γ-sarcoglycan membrane protein peptide The stepwise method consisted of the glycosylation of a soluble 7-residue peptide, a hydrophobic WALP-NVT peptide, and a γ-sarcoglycan membrane protein, all of which contained the glycosylation site Asn-Val-Thr (NVT). 32188979 6 38 gly glycosylation 1066:1078 arg1 Hyl Hyl OGER Hyl P42679 Data analysis demonstrated that (i) collagen triple helices remain in a triple-helical structure upon glycosylation of Hyl; (ii) glycosylation of Hyl modulates the peptide backbone conformation and their solvation environment in the vicinity and (iii) the attached sugars are arranged such that their hydrophilic faces are well exposed to the solvent, while their hydrophobic faces point towards the hydrophobic portions of collagen. 32188979 6 82 gly glycosylation 1039:1051 arg1 Hyl Hyl OGER Hyl P42679 Data analysis demonstrated that (i) collagen triple helices remain in a triple-helical structure upon glycosylation of Hyl; (ii) glycosylation of Hyl modulates the peptide backbone conformation and their solvation environment in the vicinity and (iii) the attached sugars are arranged such that their hydrophilic faces are well exposed to the solvent, while their hydrophobic faces point towards the hydrophobic portions of collagen. 34105348 7 47 part_of peptides 1250:1257 arg1 33 proteins proteins peptides Fterm Site proteins peptides Using the CpOGAD298N affinity column, we identified 84 highly confident O-GlcNAc modified peptides from 82 proteins in the MCF-7 cell line and 33 highly confident peptides in 33 proteins from mouse liver tissue; most of them are novel O-GlcNAc proteins and could not bind with wheat germ agglutinin (WGA). 34105348 7 78 part_of proteins 1194:1201 arg1 33 highly confident peptides proteins 33 highly confident peptides Fterm Site proteins peptides Using the CpOGAD298N affinity column, we identified 84 highly confident O-GlcNAc modified peptides from 82 proteins in the MCF-7 cell line and 33 highly confident peptides in 33 proteins from mouse liver tissue; most of them are novel O-GlcNAc proteins and could not bind with wheat germ agglutinin (WGA). 32851535 4 47 part_of N-glycosylated 1028:1041 arg1 209 N-glycosylated peptides 209 N-glycosylated 209 N-glycosylated peptides Cterm Site 209 N-glycosylated peptides Furthermore, the practical applicability of pGP/AuG is evaluated, in which 209 N-glycosylated peptides corresponding to 128 N-glycosylated proteins are identified from 1 μL human serum in three independent analysis procedures, suggesting the great potential for application in glycoproteome fields.Graphical abstract Schematic presentation of preparation for porous graphene oxide-based hydrophilic beads (pGP/AuG) with honeycomb-like microstructure. 34062155 3 58 gly glycoprotein 544:555 arg1 the glycoprotein torsinA the glycoprotein torsinA Fterm glycoprotein Here, we used the glycoprotein torsinA as a model substrate to explore the impact of ER redox homeostasis on PTMs and protein biogenesis. 34562451 4 8 part_of SP 755:756 arg1 the SP domain SP the SP domain Cterm Site SP 2147 domain Individual and combinatory site-directed mutagenesis of the glycosylated asparagine residues indicated that glycosylation of the SP domain is critical for TMPRSS13 autoactivation and catalytic activity toward one of its protein substrates, the prostasin zymogen. 32518939 1 34 part_of proteins 163:170 arg1 Epidermal growth factor-like domains proteins Epidermal growth factor-like domains Fterm Site proteins domains Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 34562451 2 52 part_of SP 468:469 arg1 the catalytic serine protease (SP) domain SP the catalytic serine protease (SP) domain Cterm Site SP 2147 domain Two of the glycosylated residues are located in the scavenger receptor cysteine-rich (SRCR) protein domain, while the remaining two sites are in the catalytic serine protease (SP) domain. 32108991 0 46 part_of VWF 31:33 arg1 VWF A-domains VWF VWF A-domains PUBTATOR Site VWF 22371 A-domains Investigating the clearance of VWF A-domains using site-directed PEGylation and novel N-linked glycosylation. 33168911 2 35 gly eNOS 410:413 arg1 novel O-GlcNAcylation sites eNOS novel O-GlcNAcylation sites PUBTATOR eNOS 24600 However, effects of glucose deprivation on eNOS O-GlcNAcylation and the presence of novel O-GlcNAcylation sites of eNOS under glucose deprivation remain unknown. 32331895 6 76 part_of bPepT2 948:953 arg1 The AA sequence bPepT2 The AA sequence Cterm Site bPepT2 Q16348 sequence The AA sequence of bPepT2 was predicted to have 12 transmembrane domains, with a large extracellular loop between the ninth and tenth transmembrane domains. 32331895 6 77 part_of have 972:975 arg1 The AA sequence AND 12 transmembrane domains The AA sequence 12 transmembrane domains domains The AA sequence of bPepT2 was predicted to have 12 transmembrane domains, with a large extracellular loop between the ninth and tenth transmembrane domains. 32315666 11 83 part_of NACP + DMAHDM 1952:1964 arg1 NACP + DMAHDM nanocomposite NACP NACP + DMAHDM nanocomposite OGER Site NACP P37840 nanocomposite When tested in a multi-species recurrent root caries model, NACP + DMAHDM nanocomposite substantially reduced root dentin demineralization and protected dentin hardness around the restorations under biofilms. 34478712 6 30 gly glycoprotein 1064:1075 arg1 reduced BSF glycoprotein binding reduced BSF glycoprotein binding Fterm glycoprotein Glycosylation defects included reduced BSF glycoprotein binding to the lectin ricin and monoclonal antibodies mAb139 and mAbCB1. 32922663 7 82 gly glycosylation 1256:1268 arg1 41 NMIBC 41 NMIBC Cterm 41 NMIBC Moreover, we identified altered glycosylation in 41 NMIBC and 21 MIBC proteins without any significant change in protein abundance levels. 33292056 9 63 gly glycoprotein 1748:1759 arg1 SARS-CoV-2 S glycoprotein SARS-CoV-2 S glycoprotein Cterm S glycoprotein 43740568 The stability of the docked complexes, i.e. PRM-A and lectins with SARS-CoV-2 S glycoprotein showed favorable intermolecular hydrogen-bond formation during the 100 ns MD simulation. 34182612 2 37 gly glycosylation 287:299 arg1 high-level membrane protein expression high-level membrane protein expression Fterm protein The following describes the effects of N-linked glycosylation on high-level membrane protein expression in yeast with an emphasis on Saccharomyces cerevisiae. 32910214 0 17 part_of multi-glycosylated 79:96 arg1 mono- and multi-glycosylated peptides/proteins multi mono- and multi-glycosylated peptides/proteins OGER Site multi peptides/proteins Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach. 34050207 0 46 gly Thr-866 75:81 arg1 O-GlcNAc modification Thr-866 O-GlcNAc modification Thr-866 Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. 32598381 10 82 part_of NA 1689:1690 arg1 the 245 NA Gly+ 245 NA the 245 NA Gly+ Cterm Site 245 NA Gly+ Finally, the 245 NA Gly+ protected from NA antibody mediated virus neutralization. 34458505 0 111 gly glycoprotein 48:59 arg1 GP5 glycoprotein gene GP5 glycoprotein gene PUBTATOR GP5 glycoprotein P40197 Isolation and molecular characterization of GP5 glycoprotein gene of Betaarterivirus suid 2 from Mizoram, India. 32851535 4 64 gly N-glycosylated 1028:1041 arg1 209 N-glycosylated peptides 209 N-glycosylated peptides peptides Furthermore, the practical applicability of pGP/AuG is evaluated, in which 209 N-glycosylated peptides corresponding to 128 N-glycosylated proteins are identified from 1 μL human serum in three independent analysis procedures, suggesting the great potential for application in glycoproteome fields.Graphical abstract Schematic presentation of preparation for porous graphene oxide-based hydrophilic beads (pGP/AuG) with honeycomb-like microstructure. 32851535 4 65 gly N-glycosylated 1073:1086 arg1 128 N-glycosylated proteins 128 N-glycosylated proteins Fterm proteins Furthermore, the practical applicability of pGP/AuG is evaluated, in which 209 N-glycosylated peptides corresponding to 128 N-glycosylated proteins are identified from 1 μL human serum in three independent analysis procedures, suggesting the great potential for application in glycoproteome fields.Graphical abstract Schematic presentation of preparation for porous graphene oxide-based hydrophilic beads (pGP/AuG) with honeycomb-like microstructure. 32737203 8 46 part_of apolipoproteins 1461:1475 arg1 composition lipoproteins composition Fterm Site lipoproteins position Second, an ApoA-1 mimetic, 5A peptide, was demonstrated to modulate the composition and concentration of apolipoproteins secreted from primary porcine RPE cells. 31588498 5 32 gly N-glycosylation 980:994 arg2 both N-glycosylation sites both N-glycosylation sites sites Complementation of the cmeA mutants with the native cmeA allele restores the WT phenotype, whereas expression of a cmeA allele with point mutations in both N-glycosylation sites is comparable to the cmeA mutants. 34327998 4 30 gly glycopeptides 584:596 arg2 all identifiable glycopeptides all identifiable glycopeptides glycopeptides This provides a facile means of representing all identifiable glycopeptides arising from a single protein "sequon" on a specific sequence, thereby enabling the comparison and searching of these distributions as routinely done for mass spectra. 32931036 4 4 gly N-glycosylation 681:695 arg1 mGlu7 function mGlu7 function Cterm mGlu7 2917 N-glycosylation is an abundant posttranslational modification that plays crucial roles in protein folding and forward trafficking, but the role of N-glycosylation in mGlu7 function remains unknown. 32393178 7 43 part_of HA 1343:1344 arg1 the three N-linked glycosylation sites HA the three N-linked glycosylation sites Cterm Site HA None sites All of the three N-linked glycosylation sites (i.e. sequons NCSV at HA 54, NHTV at HA 125, and NLSK at HA 160) identified by MTL-SGL to determine antigenic changes were experimentally validated in the H1N1 antigenic variants using mass spectrometry analyses. 32393178 7 91 part_of HA 1378:1379 arg1 the three N-linked glycosylation sites HA the three N-linked glycosylation sites Cterm Site HA None sites All of the three N-linked glycosylation sites (i.e. sequons NCSV at HA 54, NHTV at HA 125, and NLSK at HA 160) identified by MTL-SGL to determine antigenic changes were experimentally validated in the H1N1 antigenic variants using mass spectrometry analyses. 32393178 7 111 part_of HA 1358:1359 arg1 the three N-linked glycosylation sites HA the three N-linked glycosylation sites Cterm Site HA None sites All of the three N-linked glycosylation sites (i.e. sequons NCSV at HA 54, NHTV at HA 125, and NLSK at HA 160) identified by MTL-SGL to determine antigenic changes were experimentally validated in the H1N1 antigenic variants using mass spectrometry analyses. 32002580 9 15 gly glycopeptides 1405:1417 arg2 372 N-linked glycopeptides 372 N-linked glycopeptides glycopeptides A total of 372 N-linked glycopeptides corresponding to different glycoproteins are identified from only 1 μL of human serum digest. 32002580 9 20 gly glycoproteins 1446:1458 arg1 different glycoproteins different glycoproteins Fterm glycoproteins A total of 372 N-linked glycopeptides corresponding to different glycoproteins are identified from only 1 μL of human serum digest. 34839024 2 45 gly leucine-rich 309:320 arg1 leucine-rich repeats leucine leucine-rich repeats leucine In this study, a novel TLR CgToll-3 with leucine-rich repeats (LRRs) and a TIR (Toll-interleukin 1-resistance) domain was cloned in Crassostrea gigas. 32023489 3 9 gly glycoprotein 513:524 arg1 GP GP PUBTATOR GP 55819 We systematically analyzed the antibody repertoire in human survivors and identified a pair of potently neutralizing mAbs that cooperatively bound to the ebolavirus glycoprotein (GP). 32023489 3 9 gly glycoprotein 513:524 arg1 the ebolavirus glycoprotein the ebolavirus glycoprotein Fterm glycoprotein 55819 We systematically analyzed the antibody repertoire in human survivors and identified a pair of potently neutralizing mAbs that cooperatively bound to the ebolavirus glycoprotein (GP). 34925382 5 7 part_of HLA 905:907 arg1 O-GlcNAcylated HLA peptides HLA O-GlcNAcylated HLA peptides OGER Site HLA peptides This cumulative list of O-GlcNAcylated HLA peptides were derived from normal and cancerous origin, as well as tissue specimen. 32510947 4 66 gly glycopeptides 947:959 arg2 glycopeptides glycopeptides glycopeptides Upon activation using UV laser pulses, efficient photodissociation of glycopeptides is achieved with production of multiple sequence ions that enable robust and precise localization of O-GlcNAc sites. 33206527 10 99 part_of site 2071:2074 arg1 CES1 CES1 site PUBTATOR Site CES1 1066 site To investigate the regulatory effect of the N79 glycan on cellular growth, we mutated the single N-glycosylation site in CES1 from Asn to Gln (CES1-N79Q) via site-directed mutagenesis. 33647064 3 47 gly glycans 269:275 arg1 GP GP glycans PUBTATOR GP 55819 Specific N-linked glycans on the arenavirus surface glycoprotein (GP) mask important epitopes and help the virus evade antibody responses. 33647064 3 47 gly glycans 269:275 arg1 the arenavirus surface glycoprotein glycoprotein glycans Fterm glycoprotein Specific N-linked glycans on the arenavirus surface glycoprotein (GP) mask important epitopes and help the virus evade antibody responses. 33647064 3 94 gly glycoprotein 303:314 arg1 GP GP PUBTATOR GP 55819 Specific N-linked glycans on the arenavirus surface glycoprotein (GP) mask important epitopes and help the virus evade antibody responses. 33647064 3 94 gly glycoprotein 303:314 arg1 the arenavirus surface glycoprotein the arenavirus surface glycoprotein Fterm glycoprotein Specific N-linked glycans on the arenavirus surface glycoprotein (GP) mask important epitopes and help the virus evade antibody responses. 32293671 5 91 gly O-glycosylation 659:673 arg1 apolipoprotein C-III apolipoprotein C-III OGER apolipoprotein C-III P02656 All patients showed loss of O-glycosylation of apolipoprotein C-III, a non-redundant substrate for GALNT2. 32193337 7 7 gly glycosylation 1040:1052 arg1 AHCY AHCY PUBTATOR AHCY 269378 Blocking glycosylation of AHCY decreases the ratio of S-adenosylmethionine versus S-adenosylhomocysteine (SAM/SAH), reduces the level of H3K4me3, and poises mESC for differentiation. 31981375 4 36 gly glycopeptide 848:859 arg2 O-linked glycopeptide bonds O-linked glycopeptide bonds glycopeptide The results indicate that CSG-1 presented the characteristic absorption peak of polysaccharide and protein, including four monosaccharides and 17 amino acids, had O-linked glycopeptide bonds, Mw , Wn , Mw /Mn , Mp , and the z-average were 5.343 × 106 , 3.203 × 106 , 1.668, 8.911 × 106 , and 6.948 × 106 , respectively. 34636569 6 21 gly glycoforms 1253:1262 arg1 M6P M6P Intriguingly, 27 mannose-6-phosphate (M6P) glycoforms were determined with core fucosylation, and 23 of them were found with the "Y-HexNAc-Fuc" ions after manual checking. 34636569 6 21 gly glycoforms 1253:1262 arg1 27 mannose-6-phosphate 27 mannose-6-phosphate Intriguingly, 27 mannose-6-phosphate (M6P) glycoforms were determined with core fucosylation, and 23 of them were found with the "Y-HexNAc-Fuc" ions after manual checking. 32222585 3 9 part_of FcγRIIIa 510:517 arg1 the Asn-162 FcγRIIIa the Asn-162 PUBTATOR SpecificSite FcγRIIIa 2214 Asn-162 Pioneer studies on FcγR N-glycans have unveiled an additional complexity in that the N-glycan linked on the Asn-162 of FcγRIIIa was shown to be directly involved in the strong affinity for afucosylated IgG1. 32023489 5 40 part_of epitope 719:725 arg1 the GP base region epitope the GP base region region Broadly neutralizing mAb rEBOV-520 targeted a conserved epitope on the GP base region. 32023489 5 43 part_of GP 734:735 arg1 the GP base region GP the GP base region PUBTATOR Site GP 55819 region Broadly neutralizing mAb rEBOV-520 targeted a conserved epitope on the GP base region. 35004852 3 17 gly glycoprotein 594:605 arg1 a model glycoprotein a model glycoprotein Fterm glycoprotein In this work, we describe a microfluidic platform that integrates cell-free protein synthesis, glycosylation, and purification of a model glycoprotein in separate compartments where each step can be individually optimized. 35004852 3 42 gly glycosylation 551:563 arg1 a model glycoprotein a model glycoprotein Fterm glycoprotein In this work, we describe a microfluidic platform that integrates cell-free protein synthesis, glycosylation, and purification of a model glycoprotein in separate compartments where each step can be individually optimized. 32439055 0 21 gly glycopeptides 107:119 arg2 glycopeptides glycopeptides glycopeptides A hydrophilic two-dimensional titanium-based metal-organic framework nanosheets for specific enrichment of glycopeptides.