doc_id sent_index relation_id relation trigger trigger_offset arg_num arg_base_np arg_protein arg_domain arg_site arg_sugar PSource SiteSource NProtein NID SiteName sent_text 32157140 4 50 gly N-glycosylated 558:571 arg1 Gpr176 Gpr176 PUBTATOR Gpr176 Q14439 Here we show that Gpr176 is N-glycosylated. 34188486 3 3 gly unglycosylated 383:396 arg1 recombinant CenA recombinant CenA Cterm CenA (rCenA However, it seems not crucial for functional activity and secretion since the unglycosylated counterpart, recombinant CenA (rCenA), is both bioactive and secretable in Escherichia coli. 34559939 3 35 gly sialylated 552:561 arg1 CD63 CD63 OGER CD63 P08962 In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified multiple microvesicle-related sialylated proteins including CD63, a classic marker of exosomes. 32222585 0 18 gly glycoforms 78:87 arg1 various IgG1 glycoforms various IgG1 glycoforms OGER IgG1 P01857 Glycosylation of Fcγ receptors influences their interaction with various IgG1 glycoforms. 34738170 1 21 gly chain 192:196 arg1 N-linked oligosaccharides chain N-linked oligosaccharides OGER chain P06744 The glycoproteins of yeast contain a large outer chain on N-linked oligosaccharides; therefore, yeast is not suitable for producing therapeutic glycoproteins for human use. 32331895 0 55 gly N-glycosylation 43:57 arg1 bovine peptide transporter 2 bovine peptide transporter 2 PUBTATOR peptide transporter 2 Q16348 Short communication: The essential role of N-glycosylation in the transport activity of bovine peptide transporter 2. 32518939 0 12 gly modified 45:52 arg3 mouse PAMR1 AND O-Glucose mouse PAMR1 O-Glucose PUBTATOR PAMR1 210622 The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 32518939 0 12 gly modified 45:52 arg3 mouse PAMR1 AND O-Fucose mouse PAMR1 O-Fucose PUBTATOR PAMR1 210622 The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 32518939 0 12 gly modified 45:52 arg3 mouse PAMR1 AND O-GlcNAc mouse PAMR1 O-GlcNAc PUBTATOR PAMR1 210622 The single EGF-like domain of mouse PAMR1 is modified by O-Glucose, O-Fucose and O-GlcNAc. 33154460 3 25 gly glycosylated 540:551 arg1 Gc2 Gc2 PUBTATOR Gc2 83733 In contrast, Gc2 is not glycosylated. 33914740 10 11 gly non-glycosylated 2112:2127 arg1 nBer e 1 nBer e 1 PUBTATOR nBer e 1 74915 Our findings suggest that the O-linked glycosylation by P. pastoris has a small but measurable effect on the in vitro antigenicity of the rBer e 1 compared to its non-glycosylated counterpart, nBer e 1, and thus may influence its applications in diagnostics and immunotherapy. 32188979 5 42 gly glycosylation 915:927 arg1 Hyl Hyl OGER Hyl P42679 In this study, we first adapted the force field parameters for O-linkages between Hyl and carbohydrates and then investigated the influence of Hyl-O-glycosylation on the structure of type I collagen molecule, by performing comprehensive molecular dynamic simulations in explicit solvent of collagen molecule segment with and without the glycosylation of Hyl. 34155345 4 17 gly modification 506:517 arg1 PTC PTC modification OGER PTC P00740 Here we found that the upregulated O-Linked N-Acetylglucosamine Transferase (OGT) expression and O-GlcNAcylation (O-GlcNAc) modification in papillary thyroid cancer (PTC) were essential in tumor growth and metastasis. 33547363 6 46 gly glycosylated 1104:1115 arg1 SPEG SPEG OGER SPEG Q15772 Solid-phase extraction of N-linked glycosylated peptides (SPEG) revealed 118 N-linked glycosylated peptides from cells grown in high- and low-iron growth conditions. 32543352 8 69 gly glycoproteins 1135:1147 arg1 SCO-spondin SCO-spondin PUBTATOR SCO-spondin 420367 Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. 33409271 5 70 gly protein 949:955 arg1 a C-terminal 8x His-tag 2019-nCoV spike protein a C-terminal 8x His-tag PUBTATOR 2019-nCoV spike protein 43740568 The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33409271 5 70 gly protein 949:955 arg1 a FLAG-tag 2019-nCoV spike protein a FLAG-tag PUBTATOR 2019-nCoV spike protein 43740568 The fusion protein, in the vector pcDNA 4/TO, comprises an N-terminal interferon alpha 2 (IFNα2) signal peptide, an eYFP, a FLAG-tag, a human rhinovirus 3C protease (HRV3C) cleavage site, the RBD of the 2019-nCoV spike protein and a C-terminal 8x His-tag. 33997889 5 32 gly glycosylates 844:855 arg1 ComP ComP OGER ComP P49747 PglS is a pilin-specific O-linking OTase that naturally glycosylates a single protein, ComP. 32019882 7 73 gly MGL 1004:1006 arg1 truncated O-linked glycans MGL truncated O-linked glycans PUBTATOR MGL 17312 In an immunocompetent orthotopic glioma mouse model overexpressing truncated O-linked glycans (MGL ligands), high-dimensional mass cytometry revealed a wide heterogeneity of infiltrating myeloid cells with increased infiltration of PD-L1+ TAMs as well as distant alterations in the bone marrow (BM). 34069226 9 44 gly afucosylated 1336:1347 arg1 afucosylated therapeutic IgG afucosylated therapeutic IgG Cterm IgG These results confirm the potential of using afucosylated therapeutic IgG to increase their efficacy. 32922663 0 88 gly N-glycosylation 120:134 arg1 MGAM MGAM PUBTATOR MGAM 8972 Urinary glycoproteomic profiling of non-muscle invasive and muscle invasive bladder carcinoma patients reveals distinct N-glycosylation pattern of CD44, MGAM, and GINM1. 32922663 0 88 gly N-glycosylation 120:134 arg1 GINM1 GINM1 OGER GINM1 Q9NU53 Urinary glycoproteomic profiling of non-muscle invasive and muscle invasive bladder carcinoma patients reveals distinct N-glycosylation pattern of CD44, MGAM, and GINM1. 32922663 0 88 gly N-glycosylation 120:134 arg1 CD44 CD44 OGER CD44 P16070 Urinary glycoproteomic profiling of non-muscle invasive and muscle invasive bladder carcinoma patients reveals distinct N-glycosylation pattern of CD44, MGAM, and GINM1. 33689141 2 84 gly contains 263:270 arg1 GPC AND eleven N-linked glycans GPC eleven N-linked glycans OGER GPC Q78HU7 The glycoprotein complex (GPC) contains eleven N-linked glycans that play essential roles in GPC functionalities such as cleavage, transport, receptor recognition, epitope shielding, and immune response. 33806920 6 2 gly hypoglycosylated 915:930 arg1 The hypoglycosylated COX-2 The hypoglycosylated COX-2 PUBTATOR COX-2 5743 The hypoglycosylated COX-2 induced by SIN-1 showed a reduced capacity to generate prostaglandins and the activity was only partially recovered after immunoprecipitation. 32946675 10 39 gly homeostasis 1265:1275 arg1 dams dams homeostasis OGER dams Q9Y6J3 In conclusion, WTS exposure during lactation altered the milk composition and altered lipid profile, glucose homeostasis and hormonal levels in dams and pups. 34030043 3 32 part_of has 469:471 arg1 TIGIT AND N32 TIGIT residues, N32 and N101 PUBTATOR SpecificSite TIGIT 201633 residues, N32 and N101 TIGIT has two N-glycosylation residues, N32 and N101. 34030043 3 32 part_of has 469:471 arg1 TIGIT AND two N-glycosylation residues TIGIT residues, N32 and N101 PUBTATOR SpecificSite TIGIT 201633 residues, N32 and N101 TIGIT has two N-glycosylation residues, N32 and N101. 34030043 3 32 part_of has 469:471 arg1 TIGIT AND two N-glycosylation residues TIGIT residues, N32 and N101 PUBTATOR SpecificSite TIGIT 201633 residues, N32 and N101 TIGIT has two N-glycosylation residues, N32 and N101. 33608772 11 6 gly glycosylated 1774:1785 arg1 serum-IgA1 serum-IgA1 PUBTATOR IgA1 P01876 This specific glycosylated form of serum-IgA1 will expand the spectrum of aberrant glycosylation which provides valuable information to cancer glycobiology. 34155551 4 7 gly glycopeptides 856:868 arg1 a recombinant SARS-CoV-2 S glycoprotein a recombinant SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 We explore the novel use of dual-functionalized titanium (IV)-immobilized metal affinity chromatography (Ti-IMAC) material for simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293 cells. 34155551 4 30 gly glycoprotein 900:911 arg1 a recombinant SARS-CoV-2 S glycoprotein a recombinant SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 We explore the novel use of dual-functionalized titanium (IV)-immobilized metal affinity chromatography (Ti-IMAC) material for simultaneous enrichment and separation of neutral and sialyl glycopeptides of a recombinant SARS-CoV-2 S glycoprotein from HEK293 cells. 34873473 5 13 gly attached 1003:1010 arg1 EGFR AND fucosylation status EGFR fucosylation status PUBTATOR EGFR 1956 Mechanistic investigations revealed that FUT8 was involved in the alteration of fucosylation status that was attached to MET and EGFR, changing MET responses after HGF stimulation, as well as in the transactivation of EGFR. 34373697 3 45 gly isolated 537:544 arg2 A. giraldii Harms AND the homogeneous polysaccharide A. giraldii Harms the homogeneous polysaccharide OGER Harms Q9ULH0 In this study, the structural characterization of the homogeneous polysaccharide isolated from A. giraldii Harms, known as AHP-II, and its immunomodulatory effects in vivo will be studied. 34562451 3 50 gly glycosylation 528:540 arg1 TMPRSS13 TMPRSS13 PUBTATOR TMPRSS13 84000 In this study, we examined the role of N-linked glycosylation in the proteolytic activity, autoactivation, and cellular localization of TMPRSS13. 32337418 1 27 gly glycosylation 112:124 arg1 immunoglobulin G immunoglobulin G PUBTATOR immunoglobulin G 16059 N-Linked glycosylation of the fragment crystallizable (Fc) domain of immunoglobulin G (IgG) is considered a significant modulator of antibody functions, which is known to be subclass-specific. 33806146 3 12 gly contained 431:439 arg1 AAP AND polysaccharides AAP polysaccharides PUBTATOR AAP 5345 AAP contained high amounts of polysaccharides (349.83 ± 5.00 mg/g extract) with a molecular weight of 158 kDa. 33168911 3 59 gly eNOS 573:576 arg1 novel O-GlcNAcylation sites eNOS novel O-GlcNAcylation sites PUBTATOR eNOS 24600 Hence, we aim to determine the effects of glucose deprivation on O-GlcNAcylation and novel O-GlcNAcylation sites of eNOS. 33168911 3 59 gly eNOS 573:576 arg1 O-GlcNAcylation eNOS O-GlcNAcylation PUBTATOR eNOS 24600 Hence, we aim to determine the effects of glucose deprivation on O-GlcNAcylation and novel O-GlcNAcylation sites of eNOS. 35822049 8 2 gly HDAC 1527:1530 arg1 O-GlcNAcylated synaptic proteins HDAC O-GlcNAcylated synaptic proteins OGER HDAC P22760 Using pathway analyses, we found TG-dependent enrichment of O-GlcNAcylated synaptic proteins, trafficking, Notch/Wnt signaling, HDAC signaling, and circadian clock proteins. 34155551 3 58 gly glycosylation 620:632 arg1 the SARS-CoV-2 S glycoprotein the SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 In this work, we perform analytical characterization of N- and O-linked glycosylation of the SARS-CoV-2 S glycoprotein. 34155551 3 103 gly glycoprotein 654:665 arg1 the SARS-CoV-2 S glycoprotein the SARS-CoV-2 S glycoprotein PUBTATOR S glycoprotein 43740568 In this work, we perform analytical characterization of N- and O-linked glycosylation of the SARS-CoV-2 S glycoprotein. 34455356 6 94 gly glycosylation 1236:1248 arg1 DTMUV NS1 DTMUV NS1 OGER NS1 Q9Y6Y0 The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 32222585 8 39 gly afucosylated 1315:1326 arg1 afucosylated IgG1 afucosylated IgG1 OGER IgG1 P01857 More specifically, the N162 glycan positively influenced the affinity (15-fold) for afucosylated IgG1 while the N45 glycan presented a negative impact (2-fold) regardless of the IgG1 glycoforms. 32222585 8 47 gly glycoforms 1414:1423 arg1 the IgG1 glycoforms the IgG1 glycoforms OGER IgG1 P01857 More specifically, the N162 glycan positively influenced the affinity (15-fold) for afucosylated IgG1 while the N45 glycan presented a negative impact (2-fold) regardless of the IgG1 glycoforms. 31904283 8 61 gly found 1191:1195 arg1 podocalyxin AND Tn antigen podocalyxin Tn antigen PUBTATOR podocalyxin 27205 Expression of the O-glycoprotein podoplanin was lost, while Tn antigen, representing immature O-glycans, was most abundantly found on podocalyxin. 33906120 10 22 gly glycosylation 1777:1789 arg1 LRR-RLK LRR-RLK OGER RLK P42681 PNGase F digestion and glycosylation site mutations further verified the glycosylation modification and glycosylation sites of LRR receptor-like serine/threonine-protein kinase (LRR-RLK) and Beta-D-glucan exohydrolase (β-D-GEH). 33933451 3 11 gly N-glycosylation 385:399 arg1 PMP22 trafficking PMP22 trafficking PUBTATOR PMP22 Q01453 Here we characterized the role of N-glycosylation in limiting PMP22 trafficking. 32193337 5 19 gly modified 729:736 arg1 AHCY AND an O-linked β-N-acetylglucosamine sugar AHCY an O-linked β-N-acetylglucosamine sugar PUBTATOR AHCY 269378 AHCY is posttranslationally modified with an O-linked β-N-acetylglucosamine sugar (O-GlcNAcylation), which is rapidly removed upon differentiation. 34069226 4 36 gly IgG 563:565 arg1 glycan-enhancing modifications IgG glycan-enhancing modifications Cterm IgG However, it is not yet clear if glycan-enhancing modifications of human IgG translate into more effective treatment in mouse models. 34646892 14 18 gly c-Myc 1993:1997 arg1 Increased O-GlcNAcylation c-Myc Increased O-GlcNAcylation PUBTATOR c-Myc 4609 Increased O-GlcNAcylation of c-Myc with 30 mM glucose stabilized the c-Myc proteins. 34105348 3 93 gly OGA 677:679 arg1 O-GlcNAc antibody CTD110.6 OGA O-GlcNAc antibody CTD110.6 OGER OGA Q9EQQ9 Here, we compared glycan array data of the mutant of Clostridium perfringen OGA (CpOGAD298N), O-GlcNAc antibody CTD110.6, and several lectins. 34455356 7 26 gly nonglycosylated 1372:1386 arg1 the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A OGER NS1 Q9Y6Y0 Compared to the parental rDTMUV, the single mutants impaired viral multiplication in vitro, while the nonglycosylated virus rDTMUV-NS1-N130A/N175A/N207A showed a 5-fold to 178-fold decrease in viral titers and smaller plaque sizes. 34132588 5 7 gly glycoprotein 625:636 arg1 strain S2 strain S2 Cterm S2 We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion of aglB blocked S-layer glycosylation and abolished surface-associated growth. 34724770 3 19 gly O-GlcNAc 387:394 arg1 O-GlcNAcylation O-GlcNAc O-GlcNAcylation PUBTATOR O-GlcNAc 8473 Similar to phosphorylation, O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) is involved in various cell-signaling processes, including apoptosis and proliferation, with O-GlcNAc addition and removal regulated by O-GlcNAc transferase and O-GlcNAcase (OGA), respectively. 33924774 9 36 gly N-glycosylation 1195:1209 arg1 ANTXR1 ANTXR1 PUBTATOR ANTXR1 84168 Overall, our results identified N-glycosylation in ANTXR1 as a necessary post-translational modification for establishing stable interactions with SVV. 34668190 0 19 gly p65 62:64 arg1 O-GlcNAcylation p65 O-GlcNAcylation PUBTATOR p65 19697 OGT upregulates myogenic IL-6 by mediating O-GlcNAcylation of p65 in mouse skeletal muscle under cold exposure. 33914740 9 59 gly glycosylated 1766:1777 arg1 glycosylated rBer e 1 glycosylated rBer e 1 PUBTATOR rBer e 1 74915 Binding of glycosylated rBer e 1 to MR alone was found to not induce the production of IL-10 that modulates bmDC to polarise Th2 cell response by suppressing IL-12 production and DC maturation. 34478712 7 68 gly present 1189:1195 arg1 lysosomal glycoprotein p67 AND a carbohydrate epitope lysosomal glycoprotein p67 a carbohydrate epitope OGER p67 P50579 The latter bind a carbohydrate epitope present on lysosomal glycoprotein p67 that we show here consists of (-6Galβ1-4GlcNAcβ1-)≥4 poly-N-acetyllactosamine repeats. 33886622 8 69 gly N-glycosylated 1420:1433 arg1 PmCOX PmCOX OGER COX Similarly, addition of tunicamycin, which inhibits N-linked glycosylation, in PmCOX-expressing cells resulted in PmCOX protein with lower molecular mass than those obtained from untreated cells, suggesting that PmCOX was N-glycosylated. 32518939 1 72 gly modified 179:186 arg1 EGF-LDs AND N-glycans EGF-LDs N-glycans OGER EGF P01132 Epidermal growth factor-like domains (EGF-LDs) of membrane and secreted proteins can be modified by N-glycans and/or potentially elongated O-linked monosaccharides such as O-glucose (O-Glc) found at two positions (O-Glc 1 and O-Glc2), O-fucose (O-Fuc) and O-N-acetylglucosamine (O-GlcNAc). 34734627 4 14 gly glycosylation 630:642 arg1 hemopexin hemopexin PUBTATOR hemopexin 3263 We found that the efficiency of glycosylation of hemopexin was dependent upon a robust NADPH-dependent cytosolic reductive pathway, which could be mimicked by the addition of a membrane-impermeable reducing agent. 34435655 6 54 gly ERK1/2 1218:1223 arg1 global O‑GlcNAcylation ERK1/2 global O‑GlcNAcylation PUBTATOR ERK1/2 50689 OGA inhibition increased global O‑GlcNAcylation and the phosphorylation of p38 MAPK, ERK1/2 and CaMKII, whereas OGT blockade had the opposite effects. 33914740 8 49 gly present 1418:1424 arg1 rBer e 1 AND Glycans rBer e 1 Glycans PUBTATOR rBer e 1 74915 Glycans present on rBer e 1 were shown to increase the efficiency of the protein recognition and internalization by murine bone marrow-derived dendritic cells (bmDC) via C-type lectin receptors, particularly the mannose receptor (MR), compared to the non-glycosylated nBer e 1 and SFA8, a weak allergenic 2S albumin protein from sunflower seed. 33914740 8 70 gly non-glycosylated 1661:1676 arg1 the non-glycosylated nBer e 1 and SFA8 the non-glycosylated nBer e 1 and SFA8 PUBTATOR nBer e 1 74915 Glycans present on rBer e 1 were shown to increase the efficiency of the protein recognition and internalization by murine bone marrow-derived dendritic cells (bmDC) via C-type lectin receptors, particularly the mannose receptor (MR), compared to the non-glycosylated nBer e 1 and SFA8, a weak allergenic 2S albumin protein from sunflower seed. 33914740 8 70 gly non-glycosylated 1661:1676 arg1 a weak allergenic 2S albumin protein a weak allergenic 2S albumin protein OGER 2S albumin protein P07724 Glycans present on rBer e 1 were shown to increase the efficiency of the protein recognition and internalization by murine bone marrow-derived dendritic cells (bmDC) via C-type lectin receptors, particularly the mannose receptor (MR), compared to the non-glycosylated nBer e 1 and SFA8, a weak allergenic 2S albumin protein from sunflower seed. 33317052 0 26 gly Decorin 34:40 arg1 Glycosaminoglycan Modification Decorin Glycosaminoglycan Modification PUBTATOR Decorin 13179 Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly. 33317052 0 39 gly Modification 18:29 arg1 Decorin AND Glycosaminoglycan Modification Decorin Glycosaminoglycan Modification PUBTATOR Decorin 13179 Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly. 33168911 11 39 gly contained 1630:1638 arg1 eNOS AND novel O-GlcNAcylation sites eNOS novel O-GlcNAcylation sites PUBTATOR eNOS 4846 A marked increase in O-GlcNAcylation indicated that eNOS contained novel O-GlcNAcylation sites that were activated by glucose deprivation. 32193337 8 44 gly glycosylation 1236:1248 arg1 AHCY AHCY PUBTATOR AHCY 269378 In addition, blocking glycosylation of AHCY reduces somatic cell reprogramming. 34544801 4 9 gly glycosylation 658:670 arg1 the total circulating IgG the total circulating IgG Cterm IgG It specifically included adaptive immune receptor repertoire sequencing to quantify and characterize N-linked glycosylation sites in the circulating BCR repertoire, proteomics to examine glycosylation patterns of the total circulating IgG, and an exploration of human-derived recombinant autoantibodies, which were studied with mass spectrometry and Ag binding assays to respectively confirm occupation of glycosylation sites and determine whether they alter binding. 32931036 9 18 gly N-glycosylation 1230:1244 arg1 mGlu7 mGlu7 Cterm mGlu7 2917 We find that N-glycosylation of mGlu7 promotes its interaction with Elfn1, thereby enabling proper localization and stable surface expression of mGlu7 at the presynaptic active zone. 34773221 3 30 gly modified 574:581 arg1 MeCP2 AND O-linked-β-N-acetylglucosamine MeCP2 O-linked-β-N-acetylglucosamine PUBTATOR MeCP2 4204 In this study, we demonstrated that MeCP2 was dynamically modified by O-linked-β-N-acetylglucosamine (O-GlcNAc) at threonine 203 (T203), an etiologic site in RTT patients. 34773221 3 30 gly modified 574:581 arg1 MeCP2 AND O-GlcNAc MeCP2 O-GlcNAc PUBTATOR MeCP2 4204 In this study, we demonstrated that MeCP2 was dynamically modified by O-linked-β-N-acetylglucosamine (O-GlcNAc) at threonine 203 (T203), an etiologic site in RTT patients. 31904283 1 80 gly T 241:241 arg1 C1GalT1 T synthase C1GalT1 PUBTATOR T synthase 94192 Mucin-type O-linked glycosylation, a posttranslational modification affecting the stability and biophysical characteristics of proteins, requires C1GalT1 (T synthase) and its obligate, X-linked chaperone Cosmc. 33361116 0 33 gly Afucosylated 0:11 arg1 Afucosylated IgG Afucosylated IgG Cterm IgG Afucosylated IgG characterizes enveloped viral responses and correlates with COVID-19 severity. 32012886 3 29 gly sialoglycoforms 469:483 arg1 PrPC sialoglycoforms PrPC sialoglycoforms PUBTATOR PrPC 19122 Prion strains replicate by selecting substrates from a large pool of PrPC sialoglycoforms expressed by a host. 34459483 9 3 gly sialylated 1583:1592 arg1 PRG4 PRG4 OGER PRG4 Q92954 The combined results demonstrate that, in addition to platelet GPIbα, PRG4 is a highly sialylated mucin-like glycoprotein found in aortic valve vegetations and may contribute to the persistence of oral streptococci in this protected endovascular niche. 34459483 9 7 gly glycoprotein 1605:1616 arg1 PRG4 PRG4 OGER PRG4 Q92954 The combined results demonstrate that, in addition to platelet GPIbα, PRG4 is a highly sialylated mucin-like glycoprotein found in aortic valve vegetations and may contribute to the persistence of oral streptococci in this protected endovascular niche. 33523898 1 52 gly glycosylation 141:153 arg1 ER ER PUBTATOR ER 2069 Asparagine (N)-linked glycosylation is required for endoplasmic reticulum (ER) homeostasis, but how this co- and posttranslational modification is maintained during ER stress is unknown. 35822049 9 17 gly DNAJC6/AUXI 1619:1629 arg1 the O-GlcNAcylation DNAJC6 the O-GlcNAcylation PUBTATOR DNAJC6 9829 Significant changes in the O-GlcNAcylation of DNAJC6/AUXI, and PICALM, proteins that are risk factors for PD and/or AD respectively, were detected. 35822049 9 62 gly PICALM 1636:1641 arg1 the O-GlcNAcylation PICALM the O-GlcNAcylation PUBTATOR PICALM 8301 Significant changes in the O-GlcNAcylation of DNAJC6/AUXI, and PICALM, proteins that are risk factors for PD and/or AD respectively, were detected. 32958677 7 48 gly attached 1310:1317 arg1 misfolded AAT AND N-glycans misfolded AAT N-glycans PUBTATOR AAT 5265 Also, N-glycans attached to misfolded AAT are not required for accelerated degradation mediated by the unconventional system, further demonstrating its catalysis-independent nature. 31936366 0 37 gly c-Myc 29:33 arg1 Increased O-GlcNAcylation Myc Increased O-GlcNAcylation OGER Myc P01108 Increased O-GlcNAcylation of c-Myc Promotes Pre-B Cell Proliferation. 33806146 4 7 gly AAP 568:570 arg1 The main sugar composition AAP The main sugar composition PUBTATOR AAP 5345 The main sugar composition of AAP includes mannose, galactose, and glucose. 33272761 8 13 gly fucosylated 1012:1022 arg1 patatin patatin PUBTATOR patatin 102577633 Our findings suggest that patatin, a fucosylated glycoprotein, could potentially be used as a naturalactiveconstituent with anti-obesity effects. 33272761 8 15 gly glycoprotein 1024:1035 arg1 patatin patatin PUBTATOR patatin 102577633 Our findings suggest that patatin, a fucosylated glycoprotein, could potentially be used as a naturalactiveconstituent with anti-obesity effects. 33607086 8 47 part_of ACE2 1352:1355 arg1 Asn90 ACE2 Asn90 PUBTATOR AminoAcid ACE2 59272 Asn90 SMD results indicate that SARS-CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 32776095 2 41 gly glycoproteins 373:385 arg1 EGFR EGFR PUBTATOR EGFR 1956 Mucin-type O-linked glycosylation is not limited to mucins and occurs on many cell surface glycoproteins including EGFR, where the number of sites can be limited. 33197187 0 26 gly O-GlcNAcase 45:55 arg1 Nonsaccharide Inhibitors O-GlcNAcase Nonsaccharide Inhibitors PUBTATOR O-GlcNAcase 10724 Diazaspirononane Nonsaccharide Inhibitors of O-GlcNAcase (OGA) for the Treatment of Neurodegenerative Disorders. 32910282 9 3 gly β2 1790:1791 arg1 O-GlcNAcylated 2 subunits O-GlcNAcylated PUBTATOR 2 subunits 20454 Additionally, we show that the Cav α1 and β2 subunits are O-GlcNAcylated while α2δ1 is not. 32878894 10 122 part_of gp85 2472:2475 arg1 N11 gp85 N11 Cterm SpecificSite gp85 N11 In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 32114385 8 63 gly O-GlcNAc 1081:1088 arg1 IKKβ IKKβ O-GlcNAc PUBTATOR IKKβ 1147 Mutations of IKKβ (S733A) and p65 (T352A) abrogated the O-GlcNAc in IKKβ and NF-κB and inhibited AIEC LF82-induced activation of NF-κB. 32114385 8 63 gly O-GlcNAc 1081:1088 arg1 NF-κB NF-κB O-GlcNAc PUBTATOR NF-κB 4790 Mutations of IKKβ (S733A) and p65 (T352A) abrogated the O-GlcNAc in IKKβ and NF-κB and inhibited AIEC LF82-induced activation of NF-κB. 32878894 10 18 gly glycosylation 2410:2422 arg1 gp85 gp85 Cterm gp85 In addition, the glycosylation sites (N6 and N11) and cysteines (C3 and C9) of gp85 played a crucial role in the receptor binding and viral entry. 31911580 4 17 gly modified 537:544 arg3 phosphoglycerate kinase 1 AND O-GlcNAc phosphoglycerate kinase 1 O-GlcNAc PUBTATOR phosphoglycerate kinase 1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 PGK1 AND O-GlcNAc PGK1 O-GlcNAc PUBTATOR PGK1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 phosphoglycerate kinase 1 AND O-linked N-acetylglucosamine phosphoglycerate kinase 1 O-linked N-acetylglucosamine PUBTATOR phosphoglycerate kinase 1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 31911580 4 17 gly modified 537:544 arg3 PGK1 AND O-linked N-acetylglucosamine PGK1 O-linked N-acetylglucosamine PUBTATOR PGK1 5230 We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). 34668190 11 97 gly p65 1878:1880 arg1 O-GlcNAcylation p65 O-GlcNAcylation PUBTATOR p65 19697 OGT deficiency attenuated the O-GlcNAcylation, activity, and nuclear translocation of p65, resulting in downregulation of IL-6 in mouse skeletal muscle of mice exposed to cold conditions. 34711323 1 62 gly glycoprotein 143:154 arg1 Recombinant human erythropoietin Recombinant human erythropoietin PUBTATOR erythropoietin 2056 Recombinant human erythropoietin (EPO) is a complex therapeutic glycoprotein with three N- and one O-glycosylation sites. 34681302 4 2 gly SeCMP 875:879 arg1 C. militaris polysaccharides CMP C. militaris polysaccharides OGER CMP Mice were treated with a normal diet (CHOW), HFD alone, HFD plus C. militaris polysaccharides (CMP), or low/medium/high dosage of SeCMP for 8 weeks. 32012886 2 5 gly modified 359:366 arg1 PrPC AND two N-linked glycans PrPC two N-linked glycans PUBTATOR PrPC 19122 PrPC is posttranslationally modified with two N-linked glycans. 34408013 1 3 gly contains 121:128 arg1 Human IgG AND one evolutionarily conserved N-linked glycan Human IgG its Fc region one evolutionarily conserved N-linked glycan Cterm Human Ig region Human IgG contains one evolutionarily conserved N-linked glycan in its Fc region at position 297. 33914740 4 32 gly glycosylation 741:753 arg1 the rBer e 1 the rBer e 1 PUBTATOR rBer e 1 74915 We also determined whether this fungal glycosylation could affect the antigenicity and immunogenicity of the rBer e 1 by using dendritic cells (DC) as an immune cell model due to their role in modulating the immune response. 32188979 2 46 gly glycosylation 272:284 arg1 Hyl Hyl OGER Hyl P42679 Among these PTMs, glycosylation of hydroxylysine (Hyl) is the most complicated. 32598381 4 91 gly Gly- 668:671 arg1 the 245 NA glycan Gly- the 245 NA glycan Cterm Gly- To assess the effect this novel N-linked glycan has on virus fitness and antibody binding, recombinant viruses with (NA Gly+) or without (NA Gly-) the 245 NA glycan were created. 34284575 8 74 gly O-glycosylation 1039:1053 arg1 recombinant SARS-CoV-2 recombinant SARS-CoV-2 OGER SARS P49591 We utilized WR-SiO2 to comprehensively analyze the O-glycosylation profile of recombinant SARS-CoV-2 S. Twenty-seven O-glycosylation sites including 18 unambiguous sites are identified on SARS-CoV-2 S. 33370382 4 10 gly glycosylation 626:638 arg1 virus-derived Env virus-derived Env PUBTATOR Env 100616444 Here, we aimed to understand the impact of the SP diversity on glycosylation of virus-derived Env and virus neutralization by swapping SPs. 32518939 8 57 gly modified 1415:1422 arg1 isolated PAMR1 EGF-LD AND the three O-linked sugars isolated PAMR1 EGF-LD the three O-linked sugars PUBTATOR PAMR1 EGF 210622 Using click chemistry and mass spectrometry, isolated PAMR1 EGF-LD was demonstrated to be modified by the three O-linked sugars. 33206527 9 163 gly CES1 1788:1791 arg1 N79 glycan CES1 N79 glycan PUBTATOR CES1 1066 Given that the N-linked glycosyl group at Asn79 (N79 glycan) of CES1 is known to influence CES1 enzyme activity, we hypothesized that the post-translational modification of CES1 at N79 may be linked to its antiproliferative activity. 34863021 3 102 gly glycosylation 399:411 arg1 FVIII immunity FVIII immunity PUBTATOR FVIII 2157 However, knowledge of the role of FVIII asparagine (N)-linked glycosylation in FVIII immunity is limited. 34490144 0 85 gly Glycosylation 34:46 arg1 YghJ YghJ PUBTATOR YghJ 2847716 Linking inherent O-Linked Protein Glycosylation of YghJ to Increased Antigen Potential. 32157140 11 62 gly N-glycosylation 1378:1392 arg1 human GPR176 human GPR176 PUBTATOR GPR176 11245 Importantly, missense variations in the conserved N-glycosylation sites of human GPR176 (rs1473415441; rs761894953) affected N-glycosylation and thereby attenuated protein expression and cAMP-repressive activity in the cells. 34418170 6 21 gly [Ca2+ 1070:1074 arg1 O-GlcNAcylation Ca2 O-GlcNAcylation OGER Ca2 P00918 The interaction effect between O-GlcNAcylation and [Ca2+ ]i on osteogenic marker expression was determined using stable OGT knockdown MC3T3-E1 cells. 32511336 3 14 gly S 355:355 arg1 22 N-linked glycan sequons S 22 N-linked glycan sequons PUBTATOR S 43740568 SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in immune evasion and occluding immunogenic protein epitopes. 32511336 3 21 gly SARS-CoV-2 344:353 arg1 22 N-linked glycan sequons SARS 22 N-linked glycan sequons OGER SARS P49591 SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in immune evasion and occluding immunogenic protein epitopes. 33546740 3 66 gly contained 505:513 arg1 THP AND arabinose THP arabinose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND mannose THP mannose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND glucuronic acid THP glucuronic acid PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND glucose THP glucose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND galacturonic acid THP galacturonic acid PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 33546740 3 66 gly contained 505:513 arg1 THP AND galactose THP galactose PUBTATOR THP 22242 Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. 34062155 8 49 gly glycosylation 1354:1366 arg1 torsinA torsinA PUBTATOR torsinA 1861 Using a yeast torsinA expression system, we demonstrate that a specific protein disulfide isomerase, Pdi1, affects the folding and N-linked glycosylation of torsinA and torsinA∆E in a redox-dependent manner, suggesting that the acquisition of early torsinA folding intermediates is sensitive to perturbed interactions between Cys residues and the quality control machinery. 33914740 6 3 gly glycosylation 1081:1093 arg1 rBer e 1 rBer e 1 PUBTATOR rBer e 1 74915 The glycosylation on rBer e 1 was shown to elicit varying degree of antigenicity by binding to different combination of human leukocyte antigens (HLA) at different frequencies compared to nBer e 1 when tested using human DC-T cell assay. 35091346 1 31 gly found 219:223 arg1 CAPs AND O-Linked β-N-acetylglucosamine CAPs O-Linked β-N-acetylglucosamine OGER CAPs Q13938 O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 35091346 1 31 gly found 219:223 arg1 CAPs AND O-GlcNAc CAPs O-GlcNAc OGER CAPs Q13938 O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). 34373697 2 55 gly present 329:335 arg2 Harms AND The polysaccharides Harms The polysaccharides OGER Harms Q9ULH0 The polysaccharides present in A. giraldii Harms contain major bioactive substances, which have antioxidant, anticancer, and antiviral activities. 33182731 5 26 gly sialylation 1224:1234 arg1 recombinant human erythropoietin recombinant human erythropoietin PUBTATOR erythropoietin 2056 Further, utilizing enhanced intact glycopeptide identification afforded by the NGAG workflow, we found that the sugar analog 1,3,4-O-Bu3ManNAc, a "high flux" metabolic precursor for sialic acid biosynthesis, increased sialylation of secreted proteins including recombinant human erythropoietin (rhEPO). 33550377 5 8 gly O-GlcNAcylation 563:577 arg1 GCs GCs O-GlcNAcylation OGER GCs Q16739 Here, O-GlcNAcylation in GCs and its effects on GC proliferation were determined. 33361116 4 34 gly afucosylated 482:493 arg1 afucosylated IgG afucosylated IgG Cterm IgG Here, we report that afucosylated IgG (approximately 6% of total IgG in humans) are specifically formed against enveloped viruses but generally not against other antigens. 33578036 0 11 gly glycosylation 84:96 arg1 SARS-CoV-2 S SARS-CoV-2 S PUBTATOR S 43740568 Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry. 34030043 4 5 part_of TIGIT 544:548 arg1 N101 TIGIT N101 PUBTATOR SpecificSite TIGIT 201633 N101 N-glycosylation on N101 of TIGIT and, to less extent, on N32, play potent roles in PVR binding. 34260942 4 51 part_of Psme3 731:735 arg1 serine 111 Psme3 serine 111 PUBTATOR SpecificSite Psme3 19192 serine 111 Mechanistically, O-GlcNAc modification of serine 111 (S111) of Psme3 promotes degradation of Ddx6, which is essential for processing body (P-body) assembly, resulting in the maintenance of ESC pluripotent state. 32676596 6 24 gly spike-glycoprotein 1279:1296 arg1 spike-glycoprotein spike-glycoprotein PUBTATOR spike 43740568 Similarly, F-glycoprotein trimers from human parainfluenza virus-type 3 and spike-glycoprotein trimers from SARS-CoV-2 could be displayed on LuS nanoparticles with decent yield and antigenicity. 34490144 6 24 gly hyperglycosylated 927:943 arg1 YghJ YghJ PUBTATOR YghJ 2847716 Using the mass spectrometry-based method BEMAP, we demonstrate that YghJ is hyperglycosylated in ETEC and identify 54 O-linked Set/Thr residues within the 1519 amino acid primary sequence. 34342946 6 53 gly glycoprotein 1188:1199 arg1 the human myelin oligodendrocyte glycoprotein the human myelin oligodendrocyte glycoprotein PUBTATOR myelin oligodendrocyte glycoprotein 4340 While there is less structural resemblance to members of the human butyrophilin family such as BTN3A, which play a role as immune receptors, the N-terminal bovine butyrophilin subdomain shows surprising similarity to the human myelin oligodendrocyte glycoprotein, a protein exposed on the surface of myelin sheaths. 34490144 9 67 gly glycosylated 1549:1560 arg1 glycosylated YghJ glycosylated YghJ PUBTATOR YghJ 2847716 We can demonstrate by ELISA that serum from patients enrolled in an ETEC H10407 controlled infection study are significantly more reactive with glycosylated YghJ compared to the non-glycosylated variant. 34773221 0 18 part_of Threonine 36:44 arg1 MeCP2 MeCP2 Threonine PUBTATOR SpecificSite MeCP2 4204 Threonine 203 Loss of O-GlcNAcylation on MeCP2 at Threonine 203 Leads to Neurodevelopmental Disorders. 34681302 7 86 gly proteins 1327:1334 arg1 subcutaneous fat fat proteins PUBTATOR fat 12491 Furthermore, serum lipopolysaccharide-binding proteins (LBP), adiponectin level, and pro-inflammation gene expression in the colon and subcutaneous fat were inhibited, whereas anti-inflammation gene expression was improved, reflecting SeCMP-200 might mitigate obese-induced inflammation. 32518939 10 73 gly O-glycosylated 1678:1691 arg1 a triple O-glycosylated EGF-LD a triple O-glycosylated EGF-LD OGER EGF P01132 Overall, these results are consistent with the presence of a triple O-glycosylated EGF-LD in mouse PAMR1. 33863420 4 42 gly glycopeptides 1008:1020 arg1 IgG IgG Cterm IgG By using sequential elution strategy, 29 phosphopeptides and 24 glycopeptides from α-casein and IgG digests mixture could be simultaneously enriched and respectively detected through a single-step enrichment and sequential elution method. 33276951 4 39 gly Deglycosylation 455:469 arg1 SARS-CoV-2-NP SARS-CoV-2-NP OGER SARS P49591 Deglycosylation of SARS-CoV-2-NP significantly increased the number of positive tests. 32482891 2 79 gly contains 315:322 arg1 ACLP AND thrombospondin repeats ACLP a collagen-binding discoidin domain thrombospondin repeats PUBTATOR Site ACLP 165 domain ACLP contains thrombospondin repeats, a collagen-binding discoidin domain, and a catalytically inactive metallocarboxypeptidase domain. 34483834 4 12 gly glycosylation 598:610 arg1 PKR2 PKR2 PUBTATOR PKR2 246313 Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. 34105348 1 51 gly O-GlcNAc 186:193 arg1 O-GlcNAcylation O-GlcNAc O-GlcNAcylation Cterm O-GlcNAc O-GlcNAcylation is an O-linked β-N-acetyl-glucosamine (O-GlcNAc)-monosaccharide modification of serine or threonine in proteins that plays a vital role in many critical cellular processes. 33788965 11 22 gly CALHM3 1591:1596 arg1 a critical determinant CALHM3 a critical determinant PUBTATOR CALHM3 119395 However, the same mutation markedly attenuated the channel activity but not surface localization of CALHM1/3, suggesting that CALHM3 palmitoylation is a critical determinant of CALHM1/3 activity but not its formation or forward trafficking. 33914740 2 58 gly detected 428:435 arg1 the P.pastoris-derived rBer e 1 AND O-linked glycans the P.pastoris-derived rBer e 1 O-linked glycans PUBTATOR e 1 However, O-linked glycans was detected on the P.pastoris-derived rBer e 1, which is not naturally present in nBer e 1, and may contribute to the allergic sensitisation. 33886622 2 40 gly N-glycosylated 315:328 arg1 COX COX OGER COX In vertebrates and corals, COX must be N-glycosylated on at least two asparagine residues in the N-(X)-S/T motif to be catalytically active. 35091346 8 40 gly HeLa 1537:1540 arg1 1951 O-GlcNAc peptides HeLa 1951 O-GlcNAc peptides OGER HeLa P0DMC3 Deep coverage of OCAPs was achieved from HeLa cells, including 1951 O-GlcNAc peptides from 1136 O-GlcNAc chromatin-associated transcription factors and cofactors (TFCs) using HCD fragmentation and 669 O-GlcNAc sites using EThcD fragmentation. 32157140 0 74 gly glycosylation 59:71 arg1 the orphan G protein-coupled receptor Gpr176 the orphan G protein-coupled receptor Gpr176 OGER Gpr176 Q14439 Identification and functional characterisation of N-linked glycosylation of the orphan G protein-coupled receptor Gpr176. 33232205 9 60 gly glycoprotein 1833:1844 arg1 the native S glycoprotein the native S glycoprotein PUBTATOR S glycoprotein 43740568 This study revealed that the native S glycoprotein stimulated neutralizing antibodies, while bacterially-expressed S fragments did not. 33206527 1 66 gly containing 176:185 arg1 human carboxylesterase 1 AND a unique N-linked glycosyl group human carboxylesterase 1 a unique N-linked glycosyl group PUBTATOR carboxylesterase 1 1066 We previously reported that human carboxylesterase 1 (CES1), a serine esterase containing a unique N-linked glycosyl group at Asn79 (N79 CES1), is a candidate serological marker of hepatocellular carcinoma (HCC). 33065162 5 1 gly activity 922:929 arg1 RBPR2 RBPR2 activity PUBTATOR RBPR2 74152 Immunoprecipitation and dual fluorescence staining were used to explore O-GlcNAc-modified RBPR2 and RBP4 binding activity on RBPR2. 34668190 12 71 gly p65 2039:2041 arg1 O-GlcNAcylation p65 O-GlcNAcylation PUBTATOR p65 19697 Taken together, our data suggested that O-GlcNAcylation of p65 enhanced p65 activity and nuclear translocation leading to the upregulation of IL-6, which maintained energy homeostasis and promotes cell survival in mouse skeletal muscle during cold exposure. 33788965 12 45 gly N-glycosylation 1740:1754 arg1 CALHM1/3 subunits CALHM1/3 subunits PUBTATOR CALHM1/3 subunits 255022 Overall, this study characterized N-glycosylation and S-palmitoylation of CALHM1/3 subunits and clarified their differential contributions to its functional expression, providing insights into the fine control of the CALHM1/3 channel and associated physiological processes. 33154460 2 16 gly contains 242:249 arg1 Gc1 AND GalNAc Gc1 GalNAc PUBTATOR Gc1 10562 Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. 33154460 2 16 gly contains 242:249 arg1 Gc1 AND an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine Gc1 an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine PUBTATOR Gc1 10562 Gc1 contains an O-linked trisaccharide [sialic acid-galactose-N-acetylgalactosamine (GalNAc)] on the threonine420 (Thr420) residue and can be converted to a potent macrophage activating factor (GcMAF) by selective removal of sialic acid and galactose, leaving GalNAc at Thr420. 34155551 8 8 gly glycosylation 1743:1755 arg1 the spike protein the spike protein PUBTATOR spike protein 43740568 In addition, we reported on the identification of mannose-6-phosphate (M6P) glycosylation, which substantially expands the current knowledge of the spike protein's glycosylation landscape and enables future investigation into the influence of M6P glycosylation of the spike protein on its cell entry. 32337418 3 40 gly glycoforms 665:674 arg1 murine IgG Fc glycoforms murine IgG Fc glycoforms PUBTATOR IgG 16059 In this study, we established a method to quantify murine IgG Fc glycoforms normalized to the protein abundance at a subclass-specific level for various mouse strains using multiple reaction monitoring. 33597033 9 70 gly Oxidative 1574:1582 arg1 lytic polysaccharide monooxygenases Oxidative lytic polysaccharide monooxygenases Cterm Oxidative Oxidative enzymes such as laccases, peroxidases and lytic polysaccharide monooxygenases that are important for lignocellulose degradation in the terrestrial environment were present but not abundant, while a notable abundance of putative esterases (such as carbohydrate esterase family 1) associated with decoupling lignin from polysaccharides in lignocellulose was observed. 33134321 3 39 gly glycoprotein 413:424 arg1 torsinA torsinA PUBTATOR torsinA 1861 One outstanding difference between torsinA and other AAA+ ATPases is that torsinA is a glycoprotein. 34069226 6 58 gly afucosylated 852:863 arg1 afucosylated hIgG1-TA99 afucosylated hIgG1-TA99 Cterm IgG1 C57Bl/6 mice that were injected intraperitoneally with B16F10-gp75 mouse melanoma developed significantly less metastasis outgrowth after treatment with afucosylated hIgG1-TA99 compared to mice treated with wildtype hhIgG1-TA99. 33806920 7 31 gly hypoglycosylated 1090:1105 arg1 hypoglycosylated COX-2 hypoglycosylated COX-2 PUBTATOR COX-2 5743 Finally, hypoglycosylated COX-2 showed a more rapid rate of degradation compared to COX-2 induced by IL-1α and an alteration in the localization with an accumulation mainly detected in the nuclear membrane. 32846088 6 36 gly SARS-CoV-2 759:768 arg1 The O-linked glycan domain SARS The O-linked glycan domain OGER SARS P49591 The O-linked glycan domain of SARS-CoV-2 is highly positively charged, which may promote binding to negatively charged human cells. 33276951 2 36 gly glycosylation 321:333 arg1 SARS-CoV-2-NP SARS-CoV-2-NP OGER SARS P49591 We herein demonstrate that the glycosylation of SARS-CoV-2-NP masks some of its antibody epitopes. 34489534 6 71 gly N-glycosylated 1092:1105 arg1 fully N-glycosylated Piezo1 protein fully N-glycosylated Piezo1 protein PUBTATOR N-glycosylated Piezo1 protein 9780 Importantly, trafficking-defective Piezo1 variants linked to generalized lymphatic dysplasia and bicuspid aortic valve display reduced fully N-glycosylated Piezo1 protein. 34536471 6 60 gly PHB 1029:1031 arg1 polysaccharides PHB polysaccharides OGER PHB P35232 The cyanobacterium was then grown on a larger scale (10 L) to evaluate the characteristics of the produced PHB in relation to the main composition of the biomass (the content of proteins, polysaccharides, and lipids): after an incubation period of 7 days, a content of 6% of lipids (52% of which as unsaturated fatty acids with 18 carbon atoms), 12% of polysaccharides, 28% of proteins, and 46% of PHB was reached. 34646892 7 17 gly c-Myc 1086:1090 arg1 O-GlcNAcylation c-Myc O-GlcNAcylation PUBTATOR c-Myc 4609 O-GlcNAcylation of c-Myc was confirmed using immunoprecipitation and proximity ligation assay. 33689141 9 47 gly residues 1671:1678 arg1 GPC GPC residues OGER GPC Q78HU7 The glycan residues on GPC provide an immune shield for the virus, and thus represent a target for the design and development of a vaccine. 33733058 3 58 gly sialylated 779:788 arg1 angiotensin-converting enzyme 2 angiotensin-converting enzyme 2 OGER angiotensin-converting enzyme 2 Q9BYF1 SARS-CoV-2 infects human cells through interaction with sialylated receptors at the surface of epithelial cells, such as angiotensin-converting enzyme 2 (ACE2). 33900328 10 50 gly N-glycosylation 1365:1379 arg1 FIP-glu FIP-glu OGER AminoAcid FIP Q15853 In conclusion, our findings revealed a novel mechanism by which N-glycosylation of FIP-glu improved its anti-inflammatory activity through the regulation of the expression of inflammatory cytokines in RAW264.7 via inhibition of p38 MAPK phosphorylation. 33292056 5 15 gly glycoproteins 1159:1171 arg1 N-linked glycans S glycoproteins N-linked glycans Cterm S glycoproteins 43740568 CBAs possess unique carbohydrate-binding specificity, therefore, CBAs like mannose-specific plant lectins/lectin-like mimic Pradimicin-A (PRM-A) can be used for targeting N-linked glycans of S glycoproteins. 35499042 16 42 gly SP1 2299:2301 arg1 O-GlcNAcylation SP1 O-GlcNAcylation OGER SP1 P08047 Furthermore, the transcription factor SP1 specifically bound to the AQP3 promoter region, and O-GlcNAcylation of SP1 increased its stability and transcriptional regulation of AQP3 which is associated with O-GlcNAcylation of SP1. 35499042 16 53 gly SP1 2188:2190 arg1 O-GlcNAcylation SP1 O-GlcNAcylation OGER SP1 P08047 Furthermore, the transcription factor SP1 specifically bound to the AQP3 promoter region, and O-GlcNAcylation of SP1 increased its stability and transcriptional regulation of AQP3 which is associated with O-GlcNAcylation of SP1. 31936366 6 47 gly c-Myc 1326:1330 arg1 O-GlcNAcylation-dependent expression Myc O-GlcNAcylation-dependent expression OGER Myc P01108 These results strongly suggest that O-GlcNAcylation-dependent expression of c-Myc represents a new regulatory component of pre-B cell proliferation, as well as a potential therapeutic target for the treatment of pre-B cell-derived leukemia. 33964586 10 3 gly G6PDH 1807:1811 arg1 O-GlcNAc modification G6PDH O-GlcNAc modification PUBTATOR G6PDH 24377 These findings, therefore, identified HA as a promising anti-I/R strategy for the heart and proposed O-GlcNAc modification of G6PDH as a therapeutic target in ischemic heart disease. 33964586 10 32 gly modification 1791:1802 arg3 G6PDH AND O-GlcNAc modification G6PDH O-GlcNAc modification PUBTATOR G6PDH 24377 These findings, therefore, identified HA as a promising anti-I/R strategy for the heart and proposed O-GlcNAc modification of G6PDH as a therapeutic target in ischemic heart disease. 32905539 5 33 gly glycosylation 653:665 arg1 p65 p65 OGER p65 Q9H4A3 In turn, GFPT2 enhanced the glycosylation of p65, which led to the nuclear translocation of p65 and then activated NF-κB pathway. 33292056 0 85 gly glycoprotein 102:113 arg1 SARS-CoV-2 spike glycoprotein spike glycoprotein carbohydrate-binding agents PUBTATOR spike glycoprotein 43740568 Sensing the interactions between carbohydrate-binding agents and N-linked glycans of SARS-CoV-2 spike glycoprotein using molecular docking and simulation studies. 33292056 0 85 gly glycoprotein 102:113 arg1 SARS-CoV-2 spike glycoprotein spike glycoprotein N-linked glycans PUBTATOR spike glycoprotein 43740568 Sensing the interactions between carbohydrate-binding agents and N-linked glycans of SARS-CoV-2 spike glycoprotein using molecular docking and simulation studies. 33886622 9 28 gly glycosylation 1452:1464 arg1 PmCOX PmCOX OGER COX Three potential glycosylation sites of PmCOX were identified at N79, N170 and N424. 33001090 2 0 gly glycosylation 317:329 arg1 the spike (S) protein the spike (S) protein OGER S) protein Q15517 Viral invasion through envelope proteins is modulated by N-linked glycosylation in the spike (S) protein. 33933451 6 20 gly N-glycosylated 905:918 arg1 WT PMP22 WT PMP22 PUBTATOR WT PMP22 Q01453 Depletion of oligosaccharyltransferase OST-A and OST-B subunits revealed that WT PMP22 is N-glycosylated posttranslationally by OST-B, whereas L16P is cotranslationally glycosylated by OST-A. 34322281 1 81 gly N-acetylgalactosaminyl 103:124 arg1 GalNAc-T N-acetylgalactosaminyl GalNAc-T Cterm N-acetylgalactosaminyl The polypeptide N-acetylgalactosaminyl transferase (GalNAc-T) enzyme family initiates O-linked mucin-type glycosylation. 32012886 6 51 gly sialylated 903:912 arg1 PrPSc PrPSc PUBTATOR PrPSc 19122 The current work found that in 22L -infected animals, PrPSc is indeed sialylated in a region dependent manner. 34142147 5 3 gly sites 790:794 arg1 Sec31A Sec31A sites PUBTATOR Sec31A 22872 We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. 34142147 5 3 gly sites 790:794 arg1 Sec24D Sec24D sites PUBTATOR Sec24D 9871 We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. 34142147 5 3 gly sites 790:794 arg1 Sec24C Sec24C sites PUBTATOR Sec24C 9632 We identify new O-GlcNAcylation sites on Sec24C, Sec24D and Sec31A, core components of the COPII system, and provide evidence for potential nutrient-sensitive pathway regulation through site-specific glycosylation. 34490144 8 71 gly glycosylated 1345:1356 arg1 the glycosylated YghJ the glycosylated YghJ PUBTATOR YghJ 2847716 Although the glycosylation sites only constitute a minor subpopulation of the available epitopes, we observed a notable difference in the immunogenicity of the glycosylated YghJ and the non-glycosylated protein variant. 34455356 6 40 part_of NS1 1265:1267 arg1 N175 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 The NS1-WT, NS1-N130A, NS1-N175A and NS1-N207A showed a shift in migration to 37 kDa after digestion with both endoglycosidases, which further confirmed that N130, N175 and N207 were the glycosylation sites of DTMUV NS1. 33647064 6 63 part_of GPC 734:736 arg1 GPC N83 GPC GPC N83 PUBTATOR SpecificSite GPC P04921 N83 and N166 Interestingly, mutations resulting in acquisition of N-linked glycans at GPC N83 and N166 frequently occurred in late stages of the infection. 33889548 8 79 gly glycopeptides 1348:1360 arg1 HPT/HPTR HPT/HPTR PUBTATOR HPT Q9HD23 Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33889548 8 79 gly glycopeptides 1348:1360 arg1 PON1 PON1 PUBTATOR PON1 5444 Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 33889548 8 79 gly glycopeptides 1348:1360 arg1 APOH APOH PUBTATOR APOH 350 Among them, glycopeptides from APOH, HPT/HPTR, and PON1 were significantly changed in AFP-negative HCC as compared to LC. 32012886 5 10 gly PrPSc 755:759 arg1 sialylation PrPSc sialylation PUBTATOR PrPSc 19122 Toward addressing this question, the current study looked into a possibility that sialylation of PrPSc might be involved in defining selective vulnerability of brain regions. 32012886 5 26 gly sialylation 740:750 arg1 PrPSc PrPSc PUBTATOR PrPSc 19122 Toward addressing this question, the current study looked into a possibility that sialylation of PrPSc might be involved in defining selective vulnerability of brain regions. 34668190 9 52 gly p65 1671:1673 arg1 significantly increased glucose metabolism p65 significantly increased glucose metabolism PUBTATOR p65 19697 In addition, increased O-GlcNAcylation was associated with significantly increased glucose metabolism, and OGT-mediated O-GlcNAcylation of p65. 34668190 9 52 gly p65 1671:1673 arg1 OGT-mediated O-GlcNAcylation p65 OGT-mediated O-GlcNAcylation PUBTATOR p65 19697 In addition, increased O-GlcNAcylation was associated with significantly increased glucose metabolism, and OGT-mediated O-GlcNAcylation of p65. 33733058 6 25 gly ACE2 1299:1302 arg1 the sialylation status ACE2 the sialylation status OGER ACE2 Q9BYF1 We hypothesize that modulating the activity of one of them, the lysosomal sialidase NEU1, could impinge on both the sialylation status of ACE2 and other host receptors as well as the extent of lysosomal exocytosis. 33733058 6 68 gly sialylation 1277:1287 arg1 ACE2 ACE2 OGER ACE2 Q9BYF1 We hypothesize that modulating the activity of one of them, the lysosomal sialidase NEU1, could impinge on both the sialylation status of ACE2 and other host receptors as well as the extent of lysosomal exocytosis. 34625256 2 18 gly N-glycosylation 413:427 arg1 PSA PSA OGER PSA P07288 The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. 34625256 2 18 gly N-glycosylation 413:427 arg1 prostate-specific antigen prostate-specific antigen PUBTATOR prostate-specific antigen 354 The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. 33436086 8 71 gly glycosylation 1458:1470 arg1 HA HA Cterm HA These findings highlight the importance of glycosylation at site 158 of HA for the pathogenicity of the H5N6 viruses. 33270417 8 41 gly glycoprotein 1245:1256 arg1 RNase B RNase B OGER RNase B P07998 A glycoprotein, RNase B, also showed a significantly elongated deamidation half-life compared to nonglycosylated protein RNase A. 32157140 10 45 gly N-glycosylated 1312:1325 arg1 human GPR176 human GPR176 PUBTATOR GPR176 11245 We also demonstrate that human GPR176 is N-glycosylated. 34590401 7 48 part_of FEN1 797:800 arg1 352 FEN1 352 PUBTATOR SpecificSite FEN1 2237 serine 352 O-GlcNAcylation at the serine 352 of FEN1 disrupts its interaction with Proliferating Cell Nuclear Antigen (PCNA) at the replication foci, and leads to altered cell cycle, defects in DNA replication, accumulation of DNA damage, and enhanced sensitivity to DNA damage agents. 32295844 0 38 gly subunit 48:54 arg1 O-GlcNAcylation myosin phosphatase targeting subunit 1 O-GlcNAcylation PUBTATOR myosin phosphatase targeting subunit 1 4659 O-GlcNAcylation of myosin phosphatase targeting subunit 1 (MYPT1) dictates timely disjunction of centrosomes. 34030043 0 27 part_of TIGIT 31:35 arg1 TIGIT Asn32 TIGIT TIGIT Asn32 PUBTATOR AminoAcid TIGIT 201633 Asn32 and Asn101 The N-linked glycosylations of TIGIT Asn32 and Asn101 facilitate PVR/TIGIT interaction. 34934932 6 31 gly HBP 899:901 arg1 glucosamine HBP glucosamine OGER HBP P20160 Administration of glucosamine, a substrate of HBP, induces protein O-GlcNAcylation, Akt activation, and cardiomyocyte hypertrophy. 33206527 9 163 part_of CES1 1788:1791 arg1 the N-linked glycosyl group at Asn79 CES1 the N-linked glycosyl group at Asn79 PUBTATOR AminoAcid CES1 1066 Asn79 Given that the N-linked glycosyl group at Asn79 (N79 glycan) of CES1 is known to influence CES1 enzyme activity, we hypothesized that the post-translational modification of CES1 at N79 may be linked to its antiproliferative activity. 33390949 6 45 gly have 1098:1101 arg1 APS-Ⅲ AND monosaccharide residues APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33390949 6 45 gly have 1098:1101 arg1 APS-Ⅰ AND monosaccharide residues APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33390949 6 45 gly have 1098:1101 arg1 APS-Ⅰ AND monosaccharide residues APS sites monosaccharide residues OGER Site APS P07288 sites Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. 33665237 10 19 gly glycoproteins 1848:1860 arg1 the small integrin-binding ligand N-linked glycoproteins the small integrin-binding ligand N-linked glycoproteins Cterm N-linked glycoproteins 25333 This study focused primarily on the NCPs belonging to the small leucine-rich proteoglycan (SLRP) family and the small integrin-binding ligand N-linked glycoproteins (SIBLINGs). 33024038 5 113 gly glycoproteins 828:840 arg1 CD55 CD55 OGER CD55 P08174 In this work, we show that CpaA cleaves a broader range of O-linked human glycoproteins, including several glycoproteins involved in complement activation, such as CD55 and CD46. 33024038 5 113 gly glycoproteins 828:840 arg1 CD46 CD46 OGER CD46 P15529 In this work, we show that CpaA cleaves a broader range of O-linked human glycoproteins, including several glycoproteins involved in complement activation, such as CD55 and CD46. 34544801 1 73 gly glycosylation 106:118 arg1 IgG-VN-Glyc IgG-VN-Glyc Cterm IgG Elevated N-linked glycosylation of IgG V regions (IgG-VN-Glyc) is an emerging molecular phenotype associated with autoimmune disorders. 34451558 0 18 gly Remodeling 34:43 arg1 Common Bean Common Bean Remodeling OGER Common Bean Q3B7T3 Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean. 34455356 10 57 part_of NS1 2085:2087 arg1 N130 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 57 part_of NS1 2085:2087 arg1 N175 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 57 part_of NS1 2085:2087 arg1 N175 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N130 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N175 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34455356 10 85 part_of N-linked 2047:2054 arg1 N175 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 34105348 5 69 gly has 903:905 arg1 CpOGAD298N AND a GlcNAc specific binding characteristic CpOGAD298N a GlcNAc specific binding characteristic Cterm CpOGAD298N Q9EQQ9 Glycan array analysis and isothermal titration calorimetry (ITC) show that CpOGAD298N has a GlcNAc specific binding characteristic. 34677377 5 29 gly κ-casein 1018:1025 arg1 O-linked glycans -casein O-linked glycans PUBTATOR -casein 281728 Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-d-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. 34677377 5 29 gly κ-casein 1018:1025 arg1 certain oligosaccharide isomers -casein certain oligosaccharide isomers PUBTATOR -casein 281728 Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-d-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. 33168911 8 26 gly eNOS 1085:1088 arg1 low glucose-associated activation eNOS low glucose-associated activation PUBTATOR eNOS 24600 In BAECs and rat thoracic aorta, low glucose-associated activation of eNOS was accompanied by elevated O-GlcNAcylation, which did not affect O-linked serine phosphorylation at 1179/1177 residues. 33065162 2 6 gly receptor 280:287 arg1 O-GlcNAcylation RBP4 receptor O-GlcNAcylation OGER RBP4 receptor Q00724 Disruption of the retinol cascade and O-GlcNAcylation of the RBP4 receptor (STRA6) are found in diabetic kidneys. 33168911 6 74 gly eNOS 871:874 arg1 Novel O-GlcNAcylation sites eNOS Novel O-GlcNAcylation sites PUBTATOR eNOS 24600 Novel O-GlcNAcylation sites of eNOS were predicted by HPLC-MS and MS/MS Ion and determined by immunoblotting. 32337418 8 10 gly N-glycoforms 1621:1632 arg1 subclass-specific IgG Fc N-glycoforms subclass-specific IgG Fc N-glycoforms PUBTATOR IgG 16059 This report demonstrates a method with great reliability and practicality that has promising potential for the relative quantitation of subclass-specific IgG Fc N-glycoforms in multiple mouse models. 32393178 4 14 gly glycosylation 869:881 arg1 HA HA Cterm HA None RESULTS A multi-task learning sparse group least absolute shrinkage and selection operator (LASSO) (MTL-SGL) regression method was developed and applied to derive two types of predominant features including protein sequence and N-linked glycosylation in hemagglutinin (HA) affecting variations in serologic data for human and swine H1N1 IAVs. 34559939 4 39 gly α2,6-sialyltransferase 635:656 arg1 ST6Gal-I α2,6-sialyltransferase I ST6Gal-I Cterm α2,6-sialyltransferase I 170589 The silencing of α2,6-sialyltransferase I (ST6Gal-I) significantly reduced the levels of α2,6-sialylated glycoconjugates on CD63 and the surface of HCC-derived exosomes (HCC-exo). 35006749 4 46 gly glycosylated 580:591 arg1 glycosylated PD-L1 glycosylated PD-L1 PUBTATOR PD-L1 29126 Molecularly imprinted by PD-L1 N-glycans, NanoNiche can specifically recognize glycosylated PD-L1 on the tumor cell surface, thereby resulting in more efficient PD-L1 blockade. 34711323 2 65 gly Glycosylation 201:213 arg1 EPO EPO PUBTATOR EPO 2056 Glycosylation of EPO influences its safety and efficacy and is defined as a critical quality attribute. 34455356 0 55 gly glycosylation 33:45 arg1 duck Tembusu virus NS1 duck Tembusu virus NS1 OGER NS1 Q9Y6Y0 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 33272761 3 40 gly glycoprotein 293:304 arg1 Patatin Patatin PUBTATOR Patatin 102577633 Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 38 gly contained 311:319 arg1 Patatin AND fucose monosaccharides Patatin fucose monosaccharides PUBTATOR Patatin 102577633 Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 33272761 3 38 gly contained 311:319 arg1 Patatin AND mannose Patatin mannose PUBTATOR Patatin 102577633 Patatin was an O-linked glycoprotein that contained fucose monosaccharides, as well as mannose, rhamnose, glucose, galactose, xylose, and arabinose. 34863021 6 21 gly removal 895:901 arg2 BDD FVIII AND N-linked glycans BDD FVIII N-linked glycans PUBTATOR FVIII 14069 RESULTS BDD FVIII-N41G, FVIII-N239A, FVIII-N1810A, and FVIII-N2118A with confirmed removal of N-linked glycans and similar glycosylation profiles to BDD FVIII were produced. 34555499 5 92 gly glycoprotein 1043:1054 arg1 the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b PUBTATOR P-selectin glycoprotein ligand-1 Q14242 To study the specificity of the potential ovarian sulfotransferases involved, Gal3ST2 (Gal-3S)-, Gal3ST4 (Gal-3S)-, and CHST1 (Gal-6S)-encoding expression plasmids were transfected individually into CHO cells also expressing the P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b (PSGL-1/mIg G2b) fusion protein and the human core 2 transferase (GCNT1). 33073165 5 26 gly Glycosylation 885:897 arg1 Hyl Hyl OGER Hyl P42679 Glycosylation of Hyl was found to strongly down-regulate the binding of CD44 and the α3β1 integrin to collagen, while the impact on α2β1 integrin binding was more modest. 32922663 9 71 gly N-glycosylation 1557:1571 arg1 CD44 CD44 OGER CD44 P16070 Further, we identified distinct N-glycosylation pattern of CD44, MGAM, and GINM1 between NMIBC and MIBC patients, which may be associated with disease progression in bladder cancer. 32922663 9 71 gly N-glycosylation 1557:1571 arg1 MGAM MGAM PUBTATOR MGAM 8972 Further, we identified distinct N-glycosylation pattern of CD44, MGAM, and GINM1 between NMIBC and MIBC patients, which may be associated with disease progression in bladder cancer. 32922663 9 71 gly N-glycosylation 1557:1571 arg1 GINM1 GINM1 PUBTATOR GINM1 Q9NU53 Further, we identified distinct N-glycosylation pattern of CD44, MGAM, and GINM1 between NMIBC and MIBC patients, which may be associated with disease progression in bladder cancer. 32119511 5 21 gly domain 915:920 arg1 OGT OGT domain PUBTATOR OGT 8473 Truncation of the tetratricopeptide repeat domain as in OGT(4) increased selectivity for the target protein through the nanobody by reducing global elevation of O-GlcNAc levels in the cell. 34418170 12 19 gly [Ca2+ 1738:1742 arg1 O-GlcNAcylation Ca2 O-GlcNAcylation OGER Ca2 P00918 O-GlcNAcylation and [Ca2+ ]i showed an interaction effect on the expression of osteogenic markers. 33689141 3 49 gly chain 591:595 arg1 GPC GPC chain OGER GPC Q78HU7 We used three mutagenesis strategies (asparagine to glutamine, asparagine to alanine, and serine/tyrosine to alanine mutants) to abolish individual glycan chain on GPC and found that all the three strategies led to cleavage inefficiency on the 2nd (N89), 5th (N119), or 8th (N365) glycosylation motif. 35006749 2 54 gly glycosylation 313:325 arg1 PD-L1 PD-L1 PUBTATOR PD-L1 29126 However, the recognition of PD-L1 by antibodies is usually suppressed due to the N-linked glycosylation of PD-L1. 34306413 1 39 gly glycosylation 253:265 arg1 HIF-1α HIF-1α PUBTATOR HIF 3091 OBJECTIVE To investigate the correlation of the expression level of hypoxia-inducible factor-1α (HIF-1α) with the glycosylation of oral squamous cell carcinoma (OSCC). 34306413 1 39 gly glycosylation 253:265 arg1 hypoxia-inducible factor-1α hypoxia-inducible factor-1α PUBTATOR hypoxia-inducible factor-1α 3091 OBJECTIVE To investigate the correlation of the expression level of hypoxia-inducible factor-1α (HIF-1α) with the glycosylation of oral squamous cell carcinoma (OSCC). 34646892 0 104 gly c-Myc 36:40 arg1 Inhibiting Hyper-O-GlcNAcylation c-Myc Inhibiting Hyper-O-GlcNAcylation PUBTATOR c-Myc 4609 Inhibiting Hyper-O-GlcNAcylation of c-Myc accelerate diabetic wound healing by alleviating keratinocyte dysfunction. 34459483 7 60 gly sTa 1330:1332 arg1 Proteoglycan 4 sTa Proteoglycan 4 OGER sTa P50402 Proteoglycan 4 (PRG4), also known as lubricin, was a major carrier of sTa in the infected vegetations. 33603735 0 33 gly Modification 29:40 arg1 Mitochondrial Antiviral Signaling Protein AND O-Linked N-Acetylglucosamine Modification Mitochondrial Antiviral Signaling Protein O-Linked N-Acetylglucosamine Modification OGER Mitochondrial Antiviral Signaling Protein Regulates Q7Z434 O-Linked N-Acetylglucosamine Modification of Mitochondrial Antiviral Signaling Protein Regulates Antiviral Signaling by Modulating Its Activity. 33603735 0 35 gly Protein 79:85 arg1 O-Linked N-Acetylglucosamine Modification Mitochondrial Antiviral Signaling Protein Regulates O-Linked N-Acetylglucosamine Modification OGER Mitochondrial Antiviral Signaling Protein Regulates Q7Z434 O-Linked N-Acetylglucosamine Modification of Mitochondrial Antiviral Signaling Protein Regulates Antiviral Signaling by Modulating Its Activity. 32337418 6 14 gly N-glycoforms 1194:1205 arg1 subclass-specific IgG Fc N-glycoforms subclass-specific IgG Fc N-glycoforms PUBTATOR IgG 16059 The method was applied to quantify the differences in subclass-specific IgG Fc N-glycoforms between systemic sclerosis (SSc) mice and healthy control mice. 32493725 2 118 gly tau 413:415 arg1 O-GlcNAcylation tau O-GlcNAcylation PUBTATOR tau 4137 Tau is subject to O-linked N-acetylglucosamine (O-GlcNAc) modification, and O-GlcNAcylation of tau has been shown to influence tau phosphorylation and aggregation. 33168911 15 88 gly eNOS 2205:2208 arg1 O-GlcNAcylation eNOS O-GlcNAcylation PUBTATOR eNOS 4846 Glucose deprivation increases O-GlcNAcylation and activity of eNOS, potentially by the AMPK-OGT pathway, suggesting that Thr866 is a novel O-GlcNAcylation site involved in glucose-deprivation mediated eNOS activation. 34418081 7 9 gly glycosylation 1025:1037 arg1 AZU1 AZU1 PUBTATOR AZU1 566 Collectively, we found that N-linked glycosylation of AZU1 directs its loading into SEVs, thereby enabling AZU1-positive SEVs to function as potent permeabilizers of endothelial cells and leading to enhanced transendothelial migration of RCC cells. 33889548 5 60 gly glycosylation 909:921 arg1 serum PON1 serum PON1 PUBTATOR PON1 5444 Quantitative lens culinaris agglutin (LCA) ELISA was further used to confirm the difference of glycosylation on serum PON1 in liver diseases (n = 56). 33868212 0 49 part_of Small 8:12 arg1 LCG-N25 Small LCG-N25 OGER SpecificSite Small N25 A Novel Small Molecule, LCG-N25, Inhibits Oral Streptococcal Biofilm. 33607086 8 8 gly glycan 1333:1338 arg1 Asn90 Asn90 Asn90 AminoAcid Asn90 SMD results indicate that SARS-CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 33206527 6 130 gly CES1 1134:1137 arg1 the N79 glycan CES1 the N79 glycan PUBTATOR CES1 1066 This antiproliferative function of the N79 glycan of CES1 was further supported by quantitative real-time polymerase chain reaction, flow cytometry, and an apoptosis protein array assay. 33755116 0 31 gly glycoprotein 83:94 arg1 HIV-1 Env glycoprotein HIV-1 Env glycoprotein PUBTATOR HIV-1 Env glycoprotein 100616444 Development of Martini 2.2 parameters for N-glycans: a case study of the HIV-1 Env glycoprotein dynamics. 33552834 1 11 gly glycosylated 267:278 arg1 glycosylated SARS-CoV-2 spike 2 protein glycosylated SARS-CoV-2 spike 2 protein PUBTATOR spike 2 protein 43740568 The pandemic COVID-19 was caused by a novel Coronavirus-2 (SARS-CoV-2) that infects humans through the binding of glycosylated SARS-CoV-2 spike 2 protein to the glycosylated ACE2 receptor. 33552834 1 55 gly glycosylated 314:325 arg1 the glycosylated ACE2 receptor the glycosylated ACE2 receptor PUBTATOR ACE2 receptor 59272 The pandemic COVID-19 was caused by a novel Coronavirus-2 (SARS-CoV-2) that infects humans through the binding of glycosylated SARS-CoV-2 spike 2 protein to the glycosylated ACE2 receptor. 33806920 4 5 gly hypoglycosylated 670:685 arg1 hypoglycosylated COX-2 hypoglycosylated COX-2 PUBTATOR COX-2 5743 Metabolic labeling experiments using 35S or cycloheximide suggested that the formation of hypoglycosylated COX-2 was dependent on de novo synthesis of the protein rather than the deglycosylation of the native protein. 32766576 8 91 gly glycan 1255:1260 arg1 Asn90 Asn90 Asn90 AminoAcid Asn90 SMD results indicate that CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 33182731 3 8 gly glycoproteins 664:676 arg1 SPEG SPEG PUBTATOR SPEG Q15772 To overcome this pitfall, in this report we compared three glycoproteomic analysis methods; first the recently developed N-linked glycans and glycosite-containing peptides (NGAG) chemoenzymatic method, second, solid-phase extraction of N-linked glycoproteins (SPEG), and third, hydrophilic interaction liquid chromatography (HILIC) by characterizing N-linked glycosites in the secretome of Chinese hamster ovary (CHO) cells. 34069226 0 42 gly Afucosylated 0:11 arg1 Afucosylated IgG Afucosylated IgG Cterm IgG Afucosylated IgG Targets FcγRIV for Enhanced Tumor Therapy in Mice. 33023909 5 68 gly STIM1 878:882 arg1 the possible O-GlcNAcylation sites STIM1 the possible O-GlcNAcylation sites PUBTATOR STIM1 6786 Using these cells, we examined the possible O-GlcNAcylation sites of STIM1 to determine whether the sites were O-GlcNAcylated. 32482891 11 23 gly glycosylation 1907:1919 arg1 ACLP ACLP PUBTATOR ACLP 165 Our findings highlight the importance of N-linked glycosylation of ACLP for its secretion and contribute to our understanding of ACLP-dependent disease pathologies. 32012886 7 0 gly sialylated 985:994 arg1 PrPSc PrPSc PUBTATOR PrPSc 19122 PrPSc in hippocampus and cortex was more sialylated than PrPSc from thalamus and stem. 34071374 5 23 gly HAp 981:983 arg1 polysaccharides HAp polysaccharides OGER HAp Q99933 Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. 33309857 0 73 gly glycosylation 116:128 arg1 CDK11p110 CDK11p110 PUBTATOR CDK11p110 984 Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. 33788965 4 56 gly N-glycosylated 577:590 arg1 CALHM1 CALHM1 and 3 PUBTATOR CALHM1 and 3 255022 Biochemical analyses of the two subunits following site-directed mutagenesis and pharmacological treatments established that both CALHM1 and 3 were N-glycosylated at single Asn residues in their second extracellular loops. 34544801 0 86 gly Glycosylation 18:30 arg1 IgG V Regions IgG V Regions Cterm IgG V Regions Elevated N-Linked Glycosylation of IgG V Regions in Myasthenia Gravis Disease Subtypes. 31924761 7 34 gly perilipin 840:848 arg1 O-GlcNAcylation perilipin 1 O-GlcNAcylation PUBTATOR perilipin 1 103968 Loss of OGT decreases O-GlcNAcylation of lipid droplet-associated perilipin 1 (PLIN1), which leads to elevated PLIN1 phosphorylation and enhanced lipolysis. 34452648 9 21 gly glycosylation 1134:1146 arg1 integrin α5 integrin α5 PUBTATOR integrin α5 3678 C1GALT1 was able to modify O-linked glycosylation on integrin α5 and thereby modulate the activation of the PI3K/AKT pathway. 32174982 10 27 gly AMPK 1362:1365 arg1 O-GlcNAcylation AMPK O-GlcNAcylation PUBTATOR AMPK 5564 We found that O-GlcNAcylation of AMPK suppressed the activity of this regulator, thereby inhibiting ULK1 activity and autophagy. 34342946 0 42 gly glycoprotein 123:134 arg1 human myelin oligodendrocyte glycoprotein human myelin oligodendrocyte glycoprotein PUBTATOR myelin oligodendrocyte glycoprotein 4340 The extracellular region of bovine milk butyrophilin exhibits closer structural similarity to human myelin oligodendrocyte glycoprotein than to immunological BTN family receptors. 32892442 9 12 gly Akt 1577:1579 arg1 the O-GlcNAcylation Akt the O-GlcNAcylation PUBTATOR Akt 207 Moreover, OSMI-1 further promoted HBV replication by inducing autophagosome formation via inhibiting the O-GlcNAcylation of Akt and mTOR. 32892442 9 35 gly mTOR 1585:1588 arg1 the O-GlcNAcylation mTOR the O-GlcNAcylation PUBTATOR mTOR 2475 Moreover, OSMI-1 further promoted HBV replication by inducing autophagosome formation via inhibiting the O-GlcNAcylation of Akt and mTOR. 32188979 6 38 gly glycosylation 1066:1078 arg1 Hyl Hyl OGER Hyl P42679 Data analysis demonstrated that (i) collagen triple helices remain in a triple-helical structure upon glycosylation of Hyl; (ii) glycosylation of Hyl modulates the peptide backbone conformation and their solvation environment in the vicinity and (iii) the attached sugars are arranged such that their hydrophilic faces are well exposed to the solvent, while their hydrophobic faces point towards the hydrophobic portions of collagen. 32188979 6 82 gly glycosylation 1039:1051 arg1 Hyl Hyl OGER Hyl P42679 Data analysis demonstrated that (i) collagen triple helices remain in a triple-helical structure upon glycosylation of Hyl; (ii) glycosylation of Hyl modulates the peptide backbone conformation and their solvation environment in the vicinity and (iii) the attached sugars are arranged such that their hydrophilic faces are well exposed to the solvent, while their hydrophobic faces point towards the hydrophobic portions of collagen. 32931036 5 8 gly N-glycosylated 769:782 arg1 mGlu7 mGlu7 Cterm mGlu7 2917 In this study, we find that mGlu7 is N-glycosylated at four asparagine residues in heterologous cells and rat cultured neurons. 34348917 9 45 gly modified 1332:1339 arg1 RUNX1 AND O-GlcNAc RUNX1 O-GlcNAc PUBTATOR RUNX1 50662 RUNX1 can be modified by O-GlcNAc, and that the modification is enhanced in a high glucose environment. 32157140 5 37 gly N-glycosylation 679:693 arg1 endogenous Gpr176 endogenous Gpr176 PUBTATOR Gpr176 Q14439 Peptide-N-glycosidase treatment of mouse hypothalamus extracts revealed that endogenous Gpr176 undergoes N-glycosylation. 32222585 3 23 gly afucosylated 580:591 arg1 afucosylated IgG1 afucosylated IgG1 OGER IgG1 P01857 Pioneer studies on FcγR N-glycans have unveiled an additional complexity in that the N-glycan linked on the Asn-162 of FcγRIIIa was shown to be directly involved in the strong affinity for afucosylated IgG1. 32987930 4 36 gly glycoproteins 678:690 arg1 the E1 and E2 glycoproteins the E1 and E2 glycoproteins PUBTATOR E1 and E2 glycoproteins 6080 In this study, we mutated the N-glycosylation consensus motifs of the E1 and E2 glycoproteins of a SAV3 infectious clone using site-directed mutagenesis. 32766576 8 35 part_of ACE2 1274:1277 arg1 Asn90 ACE2 Asn90 PUBTATOR AminoAcid ACE2 59272 Asn90 SMD results indicate that CoV-2 RBD interacts with the N-linked glycan on Asn90 of ACE2. 33924774 8 75 gly deglycosylation 1091:1105 arg1 ANTXR1 ANTXR1 PUBTATOR ANTXR1 84168 Cell blocking, co-immunoprecipitation, and plaque formation assays confirmed that deglycosylation of ANTXR1 prevents SVV attachment and subsequent entry. 33168911 2 35 gly eNOS 410:413 arg1 novel O-GlcNAcylation sites eNOS novel O-GlcNAcylation sites PUBTATOR eNOS 24600 However, effects of glucose deprivation on eNOS O-GlcNAcylation and the presence of novel O-GlcNAcylation sites of eNOS under glucose deprivation remain unknown. 34069226 7 5 gly Afucosylated 928:939 arg1 Afucosylated human IgG1 Afucosylated human IgG1 OGER IgG1 P01857 Afucosylated human IgG1 showed stronger interaction with the murine FcγRIV, the mouse orthologue of human FcγRIIIa, indicating that this glycan change is functionally conserved between the species. 32931036 7 63 gly Deglycosylated 964:977 arg1 Deglycosylated mGlu7 Deglycosylated mGlu7 Cterm Deglycosylated mGlu7 2917 Deglycosylated mGlu7 is retained in the ER, obstructing expression on the cell surface, and is degraded through the autophagolysosomal degradation pathway. 34455356 10 61 gly glycosylation 2056:2068 arg1 DTMUV NS1 DTMUV NS1 OGER NS1 Q9Y6Y0 Overall, our results indicated that N130, N175 and N207 are N-linked glycosylation sites of DTMUV NS1, which play crucial roles in viral multiplication, viremia and virulence in vitro and in vivo. 33889548 6 95 gly N-glycopeptides 1010:1024 arg1 PON1 PON1 PUBTATOR PON1 5444 Then, the alteration of site-specific intact N-glycopeptides of PON1 was comprehensively assessed by using Immunoprecipitation (IP) and mass spectrometry based 16O/18O C-terminal labeling quantification method to distinguish AFP-negative HCC from LC patients in a validation set (n = 64). 32922663 7 82 gly glycosylation 1256:1268 arg1 41 NMIBC 41 NMIBC Cterm 41 NMIBC Moreover, we identified altered glycosylation in 41 NMIBC and 21 MIBC proteins without any significant change in protein abundance levels. 33292056 9 63 gly glycoprotein 1748:1759 arg1 SARS-CoV-2 S glycoprotein SARS-CoV-2 S glycoprotein Cterm S glycoprotein 43740568 The stability of the docked complexes, i.e. PRM-A and lectins with SARS-CoV-2 S glycoprotein showed favorable intermolecular hydrogen-bond formation during the 100 ns MD simulation. 33065162 8 62 gly RBPR2 1248:1252 arg1 O-GlcNAcylation RBPR2 O-GlcNAcylation PUBTATOR RBPR2 74152 RESULTS Disruption of retinol cascade, RBP4 overproduction, O-GlcNAcylation of RBPR2, decreased RBP4 binding activity on RBPR2 and inflammation were found in livers of db/db and ob/ob mice and high glucose-cultured hepatocytes. 33272761 4 42 gly had 426:428 arg1 Patatin AND a fucosylated glycan structural feature Patatin a fucosylated glycan structural feature PUBTATOR Patatin 102577633 Patatin had a fucosylated glycan structural feature, which strongly bound AAL (Aleuria aurantia Leukoagglutinin), a known fucose binding lectin. 34071949 6 44 gly MDC1 1080:1083 arg1 O-GlcNAcylation MDC1 O-GlcNAcylation PUBTATOR MDC1 9656 We confirmed that O-GlcNAcylation of MDC1 is increased upon irradiation and identified additional repair factors related to Homologous Recombination (HR), CtIP and BRCA1, which were increasingly O-GlcNAcyated upon irradiation. 34711323 5 77 gly glycoforms 730:739 arg1 EPO glycoforms EPO glycoforms PUBTATOR EPO 2056 Alternatively, comprehensive insights into the glycoform heterogeneity of intact EPO are obtained using ESI MS-based methods with or without upfront separation of EPO glycoforms. 34711323 5 85 gly heterogeneity 620:632 arg1 intact EPO intact EPO PUBTATOR EPO 2056 Alternatively, comprehensive insights into the glycoform heterogeneity of intact EPO are obtained using ESI MS-based methods with or without upfront separation of EPO glycoforms. 32931036 6 42 gly N-glycosylation 880:894 arg1 mGlu7 mGlu7 Cterm mGlu7 2917 We demonstrate that N-glycosylation is essential for forward transport and surface expression of mGlu7. 34555499 10 70 gly glycoproteins 1951:1963 arg1 OC glycoproteins OC glycoproteins Cterm OC glycoproteins 3958 The data displays a complex sulfation pattern of O-glycans on OC glycoproteins and that aggressiveness of the cancer is associated with a decreased expression of the Gal3ST2 transferase. 34458505 0 111 gly glycoprotein 48:59 arg1 GP5 glycoprotein gene GP5 glycoprotein gene PUBTATOR GP5 glycoprotein P40197 Isolation and molecular characterization of GP5 glycoprotein gene of Betaarterivirus suid 2 from Mizoram, India. 33073165 2 13 gly glycosylated 420:431 arg1 glycosylated 5-hydroxylysine 5-hydroxylysine (Hyl OGER 5-hydroxylysine (Hyl P42679 To fully investigative collagen biochemistry, one needs to assemble peptides that possess glycosylated 5-hydroxylysine (Hyl). 34260942 0 34 gly Psme3 33:37 arg1 Site-specific O-GlcNAcylation Psme3 Site-specific O-GlcNAcylation PUBTATOR Psme3 19192 Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis. 32931036 4 4 gly N-glycosylation 681:695 arg1 mGlu7 function mGlu7 function Cterm mGlu7 2917 N-glycosylation is an abundant posttranslational modification that plays crucial roles in protein folding and forward trafficking, but the role of N-glycosylation in mGlu7 function remains unknown. 34262722 11 114 gly mannose 1740:1746 arg1 BLC-1 BLC mannose OGER BLC O43927 The proportions of glucose, mannose in BLC-1 and BLC-2 were 47.68%, 39.18%, and 76.59%, 6.86%, respectively. 34262722 11 130 gly glucose 1731:1737 arg1 BLC-1 BLC glucose OGER BLC O43927 The proportions of glucose, mannose in BLC-1 and BLC-2 were 47.68%, 39.18%, and 76.59%, 6.86%, respectively. 34195388 6 65 gly O-glycosylation 838:852 arg1 Fos Fos PUBTATOR Fos 14281 Specificity protein 1 (Sp1) expression and O-glycosylation, as well as the role of AP-1 members Jun and Fos in the regulation of GSTP expression, were also assessed. 33921866 8 9 gly proliferator 1438:1449 arg1 Fructose-induced upregulation peroxisome proliferator activated receptor α Fructose-induced upregulation PUBTATOR peroxisome proliferator activated receptor α 25747 Fructose-induced upregulation of complex II-driven mitochondrial respiration, peroxisome proliferator-activated receptor-gamma coactivator 1 alpha, and peroxisome proliferator activated receptor α also persisted after switching to control diet. 33921866 8 63 gly alpha 1416:1420 arg1 Fructose-induced upregulation peroxisome proliferator-activated receptor-gamma coactivator 1 alpha Fructose-induced upregulation PUBTATOR peroxisome proliferator-activated receptor-gamma coactivator 1 alpha 83516 Fructose-induced upregulation of complex II-driven mitochondrial respiration, peroxisome proliferator-activated receptor-gamma coactivator 1 alpha, and peroxisome proliferator activated receptor α also persisted after switching to control diet. 33682335 2 21 gly glycoforms 329:338 arg1 ribonuclease B ribonuclease B Cterm ribonuclease B Separations of the glycoforms of ribonuclease B compared to its released glycans were studied using a commercial hydrophilic interaction liquid chromatography column. 32023489 3 9 gly glycoprotein 513:524 arg1 GP GP PUBTATOR GP 55819 We systematically analyzed the antibody repertoire in human survivors and identified a pair of potently neutralizing mAbs that cooperatively bound to the ebolavirus glycoprotein (GP). 32331895 11 67 gly N-glycosylation 1997:2011 arg1 bPepT2 bPepT2 Cterm bPepT2 Q16348 In summary, this study demonstrated that N-glycosylation is critical for the function of bPepT2. 34455356 0 45 part_of N-linked 24:31 arg1 N130 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 45 part_of N-linked 24:31 arg1 N207 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 45 part_of N-linked 24:31 arg1 N207 N N130, N175 and N207 Cterm SpecificSite N N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N130 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N207 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 34455356 0 80 part_of NS1 75:77 arg1 N207 NS1 N130, N175 and N207 OGER SpecificSite NS1 Q9Y6Y0 N130, N175 and N207 N130, N175 and N207 are N-linked glycosylation sites of duck Tembusu virus NS1 that are important for viral multiplication, viremia and virulence in ducklings. 33168911 13 98 gly O-GlcNAcylation 1948:1962 arg1 His-tagged human eNOS His-tagged human eNOS PUBTATOR eNOS 4846 Conversely, in His-tagged human eNOS carrying a mutation at Thr866, O-GlcNAcylation was unaffected by glucose deprivation. 33950562 8 0 gly JunB 1131:1134 arg1 Target protein O-GlcNAcylation JunB Target protein O-GlcNAcylation OGER JunB P17275 Basic Protocol 1: Target protein O-GlcNAcylation of JunB using nanobody-OGT Basic Protocol 2: Target protein deglycosylation of Nup62 using nanobody-splitOGA Alternate Protocol: Verification of the O-GlcNAc state of a tagged target protein through chemoenzymatic labeling Support Protocol: Cloning of new nanobody-OGT/nanobody-splitOGA and target protein pairs. 33950562 8 54 gly deglycosylation 1188:1202 arg1 Nup62 Nup62 OGER Nup62 P37198 Basic Protocol 1: Target protein O-GlcNAcylation of JunB using nanobody-OGT Basic Protocol 2: Target protein deglycosylation of Nup62 using nanobody-splitOGA Alternate Protocol: Verification of the O-GlcNAc state of a tagged target protein through chemoenzymatic labeling Support Protocol: Cloning of new nanobody-OGT/nanobody-splitOGA and target protein pairs. 33647064 3 47 gly glycans 269:275 arg1 GP GP glycans PUBTATOR GP 55819 Specific N-linked glycans on the arenavirus surface glycoprotein (GP) mask important epitopes and help the virus evade antibody responses. 33647064 3 94 gly glycoprotein 303:314 arg1 GP GP PUBTATOR GP 55819 Specific N-linked glycans on the arenavirus surface glycoprotein (GP) mask important epitopes and help the virus evade antibody responses. 32293671 5 91 gly O-glycosylation 659:673 arg1 apolipoprotein C-III apolipoprotein C-III OGER apolipoprotein C-III P02656 All patients showed loss of O-glycosylation of apolipoprotein C-III, a non-redundant substrate for GALNT2. 32193337 7 7 gly glycosylation 1040:1052 arg1 AHCY AHCY PUBTATOR AHCY 269378 Blocking glycosylation of AHCY decreases the ratio of S-adenosylmethionine versus S-adenosylhomocysteine (SAM/SAH), reduces the level of H3K4me3, and poises mESC for differentiation. 33523898 6 23 gly glycoprotein 1194:1205 arg1 ER glycoprotein quality control ER glycoprotein quality control PUBTATOR ER glycoprotein 2069 The fidelity of N-glycosylation is not static in both nontransformed and tumor cells, and the TRAP complex regulates ER glycoprotein quality control under conditions of stress. 33370382 5 39 gly glycans 871:877 arg1 N88 N88 N88 SpecificSite N88 Analyses of site-specific glycans revealed that SP swapping altered Env glycan content and occupancy on multiple N-linked glycosites, including conserved N156 and N160 glycans in the V1V2 region at the Env trimer apex and N88 at the trimer base. 32099050 4 4 gly phospholipase 807:819 arg1 the allergen Api m 1 phospholipase A2 the allergen Api m 1 OGER phospholipase A2 P04054 Tandem mass spectrometry identified the cross-reactive allergens as Der f 15 in HDM, two homologues of the Periplaneta americana cockroach allergen Cr-PI/Per a 3 in ACR and two isoforms of the allergen Api m 1 (phospholipase A2: PLA2) in HBV. 32222585 3 9 part_of FcγRIIIa 510:517 arg1 the Asn-162 FcγRIIIa the Asn-162 PUBTATOR SpecificSite FcγRIIIa 2214 Asn-162 Pioneer studies on FcγR N-glycans have unveiled an additional complexity in that the N-glycan linked on the Asn-162 of FcγRIIIa was shown to be directly involved in the strong affinity for afucosylated IgG1. 34489534 4 38 gly glycosylated 751:762 arg1 the fully glycosylated mature Piezo1 protein the fully glycosylated mature Piezo1 protein PUBTATOR Piezo1 protein 9780 Both mutation of these asparagines (N2294Q/N2331Q) and treatment with an enzyme that hydrolyses N-linked oligosaccharides (PNGaseF) eliminates the fully glycosylated mature Piezo1 protein. 34348917 5 71 gly RUNX1 989:993 arg1 The O-GlcNAc modification RUNX1 The O-GlcNAc modification PUBTATOR RUNX1 50662 The O-GlcNAc modification of RUNX1 was detected by immunoprecipitation.