doc_id sent_index relation_id relation trigger trigger_offset arg_num arg_base_np arg_protein arg_domain arg_site arg_sugar PSource SiteSource NProtein NID SiteName sent_text 25466456 2 4 gly non-glycosylated 356:371 arg1 non-glycosylated (HFBI, m.w. ca. 7 kDa) vs glycosylated (FpHYD5, m.w. ca. 10 kDa) proteins non-glycosylated (HFBI, m.w. ca. 7 kDa) vs glycosylated (FpHYD5, m.w. ca. 10 kDa) proteins Fterm proteins The two hydrophobins are featured as non-glycosylated (HFBI, m.w. ca. 7 kDa) vs glycosylated (FpHYD5, m.w. ca. 10 kDa) proteins. 25466456 2 36 gly glycosylated 399:410 arg1 non-glycosylated (HFBI, m.w. ca. 7 kDa) vs glycosylated (FpHYD5, m.w. ca. 10 kDa) proteins non-glycosylated (HFBI, m.w. ca. 7 kDa) vs glycosylated (FpHYD5, m.w. ca. 10 kDa) proteins Fterm proteins The two hydrophobins are featured as non-glycosylated (HFBI, m.w. ca. 7 kDa) vs glycosylated (FpHYD5, m.w. ca. 10 kDa) proteins. 27031228 3 21 part_of containing 625:634 arg1 the epitope AND glycosylated Thr52 the epitope glycosylated Thr52 Thr52 Although various anti-hPDPN monoclonal antibodies (mAbs) have been generated, no specific mAb has been reported to target the epitope containing glycosylated Thr52. 25722146 1 44 gly composition 344:354 arg1 the polysaccharide position the polysaccharide position The effect of high levels of the polysaccharide Saccharomyces cerevisiae yeast strain (HPS) and another conventional yeast strain (FERM) on the polysaccharide and phenolic composition of Syrah red wines during alcoholic fermentation and subsequent aging on lees, with or without oak wood chips, and on inactive dry yeast was investigated. 28342150 4 37 gly sialylation 923:933 arg1 N-glycans N-glycans β-1,4-Galactosyltransferase 1 (β4GalT1) is known as one of type II transmembrane enzymes that transfer galactose in a β-1, 4 linkage to accepter sugars, and a key enzyme for further sialylation of N-glycans. 24872420 8 70 part_of V1/V2 1461:1465 arg1 the V1/V2 domain V1/V2 the V1/V2 domain Cterm Site V1/V2 domain We also describe the properties of glycopeptide scaffolds from the V1/V2 domain also expressed with mannose-5 glycans. 25594223 5 2 part_of gp41 692:695 arg1 a functional fragment gp41 a functional fragment Cterm Site gp41 fragment SL-ENF bound to a functional fragment of the HIV fusogenic protein gp41 and formed complexes with high affinity and α-helicity, revealing the mechanism behind its potent antiviral activity. 28656506 0 50 gly glycosylated 30:41 arg1 proline rich antibacterial peptide Drosocin peptide PUBTATOR Drosocin 36635 peptide Comparing naturally occurring glycosylated forms of proline rich antibacterial peptide, Drosocin. 25261472 9 60 gly glycoprotein 1437:1448 arg1 glycoprotein sorting glycoprotein sorting Fterm glycoprotein Taken together, our data provide strong evidence that N-linked glycosylation directs glycoprotein sorting into EMVs. 26657283 2 2 gly carries 431:437 arg1 the central chemokine receptor AND polysialic acid the central chemokine receptor polysialic acid Fterm receptor Here we show that CCR7, the central chemokine receptor controlling immune cell trafficking to secondary lymphatic organs, carries polysialic acid. 25832992 8 41 gly glycoproteins 1473:1485 arg1 glycoproteins glycoproteins Fterm glycoproteins Our results provide an alternative method for the mass production of active enzymes involved in the study of glycoproteins. 26123275 3 0 gly domains 470:476 arg1 hybrid GAG chains domains hybrid GAG chains domains In this work, for the structural characterization of DS- and CS-rich domains in hybrid GAG chains extracted from neural tissue, we have developed an advanced approach based on high-resolution mass spectrometry (MS) using nanoelectrospray ionization Orbitrap in the negative ion mode. 26721331 7 21 gly sialylated 1062:1071 arg1 α2-3 linked sialylated N-glycans α2-3 linked sialylated N-glycans A shift from α2-6 towards α2-3 linked sialylated N-glycans was also observed. 27733679 0 26 gly Glycosylation 104:116 arg1 Dystroglycan Dystroglycan The Muscular Dystrophy Gene TMEM5 Encodes a Ribitol β1,4-Xylosyltransferase Required for the Functional Glycosylation of Dystroglycan. 24982189 6 35 part_of sequence 902:909 arg1 GN GN sequence Cterm Site GN P46976 sequence We describe the crystal structure of Caenorhabditis elegans GS in complex with a minimal GS targeting sequence in GN and show that a 34-residue region of GN binds to a conserved surface on GS that is distinct from previously characterized allosteric and binding surfaces on the enzyme. 24982189 6 59 part_of GN 954:955 arg1 a 34-residue region GN a 34-residue region Cterm Site GN P46976 region We describe the crystal structure of Caenorhabditis elegans GS in complex with a minimal GS targeting sequence in GN and show that a 34-residue region of GN binds to a conserved surface on GS that is distinct from previously characterized allosteric and binding surfaces on the enzyme. 26417422 1 28 gly N-glycans 128:136 arg1 proteins proteins N-glycans Fterm proteins Quantification, characterization and biofunctional studies of N-glycans on proteins remain challenging tasks due to complexity, diversity and low abundance of these glycans. 24872420 10 17 part_of V1/V2 1710:1714 arg1 A244 V1/V2 fragments A244 V1/V2 A244 V1/V2 fragments Cterm Site A244 V1/V2 fragments We further show that immunization with A244 V1/V2 fragments alone, or in a prime/boost regimen with gp120, enhanced the antibody response to sequences in the V1/V2 domain associated with protection in the RV144 trial. 24872420 10 50 part_of V1/V2 1824:1828 arg1 the V1/V2 domain V1/V2 the V1/V2 domain Cterm Site V1/V2 domain We further show that immunization with A244 V1/V2 fragments alone, or in a prime/boost regimen with gp120, enhanced the antibody response to sequences in the V1/V2 domain associated with protection in the RV144 trial. 24872420 10 51 part_of A244 1705:1708 arg1 A244 V1/V2 fragments A244 V1/V2 A244 V1/V2 fragments Cterm Site A244 V1/V2 fragments We further show that immunization with A244 V1/V2 fragments alone, or in a prime/boost regimen with gp120, enhanced the antibody response to sequences in the V1/V2 domain associated with protection in the RV144 trial. 24370475 2 19 gly glycosylated 489:500 arg1 glycosylated serum protein glycosylated serum protein Fterm protein The analysis of fasting blood glucose, glycosylated serum protein and serum insulin levels showed that DHP, DOP and DNP, but not DCP, possessed significant hypoglycemic effect with the decreasing order of DHP>DNP>DOP. 25151386 9 45 gly glycosylation 1412:1424 arg1 ion channel availability ion channel availability Fterm channel This chapter will describe ion channel and ion pump structures with a focus on the functional effects of glycosylation on ion channel availability and function, and effects of alterations in glycosylation on nervous system function. 29152974 5 67 part_of CS 1151:1152 arg1 the [CEL + CS] composite CS the [CEL + CS] composite PUBTATOR Site CS 1431 composite Electron paramagnetic resonance (EPR) suggests that some 25% of the EPR-detectable Cu(II) is present as a monomeric species, chemically anchored to the substrate by two or more nitrogen atoms, and, further, adopts a unique spatially oriented conformation when incorporated into the [CEL + CS] composite but not in the [CEL + KER] composite. 28371609 4 101 gly Asn297-linked 941:953 arg1 the Asn297-linked glycan Asn297 the Asn297-linked glycan Asn297 Western blot analysis with anti-α1,3-fucose and anti-xylose antibodies, as well as a test with peptide-N-glycosidase F, confirmed the absence of α1,3-fucose and xylose in the Asn297-linked glycan of PPB-ΔXTFT and TPB-ΔXTFT. 28189248 5 9 part_of /O-BNT 1006:1011 arg1 /O-BNT composite CS(3)/O-BNT /O-BNT composite PUBTATOR Site CS(3)/O-BNT 91977 composite While the most negative effect on ζ-potential of the composites was reached with SDS, which reduced the value of ζ-potential to -39mV for CS(1)/O-BNT composite, the most positive effect was monitored with CTAB (ζ=+40mV) for CS(3)/O-BNT composite. 28189248 5 40 part_of /O-BNT 920:925 arg1 ζ-potential to -39mV for CS(1)/O-BNT composite CS(1)/O-BNT ζ-potential to -39mV for CS(1)/O-BNT composite PUBTATOR Site CS(1)/O-BNT 1442 composite While the most negative effect on ζ-potential of the composites was reached with SDS, which reduced the value of ζ-potential to -39mV for CS(1)/O-BNT composite, the most positive effect was monitored with CTAB (ζ=+40mV) for CS(3)/O-BNT composite. 28959962 3 80 gly glycoproteins 447:459 arg1 complex glycoproteins complex glycoproteins Fterm glycoproteins Despite the importance of glycosylation in biology, the identification and functional validation of complex glycoproteins has remained largely unexplored. 29150937 1 4 gly glycoprotein 195:206 arg1 SOSIP.664 HIV-1 envelope glycoprotein SOSIP.664 HIV-1 envelope glycoprotein Fterm glycoprotein We describe the properties of BG505 SOSIP.664 HIV-1 envelope glycoprotein trimers produced under current Good Manufacturing Practice (cGMP) conditions. 24531467 1 55 gly glycosylated 158:169 arg1 endo-β-1,3-glucanase endo-β-1,3-glucanase Fterm endo-β-1,3-glucanase Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. 25545171 2 49 part_of Multiple 197:204 arg1 medium composition Multiple medium composition Cterm Site Multiple position Multiple factors including medium composition and process conditions impact protein glycosylation and characterizing cellular response to these changes is essential to understand the underlying relationships. 27413183 1 70 gly glycoproteins 226:238 arg1 newly synthesized glycoproteins newly synthesized glycoproteins Fterm glycoproteins Calreticulin is a lectin chaperone of the endoplasmic reticulum that interacts with newly synthesized glycoproteins by binding to Glc1Man9GlcNAc2 oligosaccharides as well as to the polypeptide chain. 27474680 12 32 part_of [CEL+KER 1930:1937 arg1 [CEL+KER] composites CEL [CEL+KER] composites PUBTATOR Site CEL P19835 composites These results together with our previous finding that [CEL+KER] composites can control release of drug such as ciprofloxacin clearly indicate that these composites can potentially be used as wound dressing. 28407380 7 29 gly glycoprotein 1481:1492 arg1 resulting glycoprotein profiles resulting glycoprotein profiles Fterm glycoprotein The strength of our N-glycosylation mutant platform is the broad spectrum of resulting glycoprotein profiles and altered physiological phenotypes that can be produced from single, double, triple and quadruple mutants. 28076415 12 7 part_of gp120 2170:2174 arg1 the gp120 C terminus gp120 C the gp120 C terminus PUBTATOR Site gp120 C 155971 terminus Altogether, this study expands the region of the HIV-1 gp120-gp41 quaternary interface that is a target for broadly neutralizing antibodies and identifies a set of mutations in the gp120 C terminus that exposes the membrane-proximal external region of gp41, with potential utility in HIV vaccine design. 28076415 12 77 part_of C 2176:2176 arg1 the gp120 C terminus gp120 C the gp120 C terminus PUBTATOR Site gp120 C 155971 terminus Altogether, this study expands the region of the HIV-1 gp120-gp41 quaternary interface that is a target for broadly neutralizing antibodies and identifies a set of mutations in the gp120 C terminus that exposes the membrane-proximal external region of gp41, with potential utility in HIV vaccine design. 28473830 6 47 gly glycosylation 699:711 arg2 the HA glycosylation sites the HA glycosylation sites sites We also decided to change neither the number nor the position of the HA glycosylation sites but only the glycan length. 27524389 1 28 gly glycoproteins 155:167 arg1 glycoproteins glycoproteins Fterm glycoproteins Carbohydrate-protein interactions occur through glycoproteins, glycolipids, or polysaccharides displayed on the cell surface with lectins. 28955895 6 33 part_of HCV 967:969 arg1 the protein N-glycosylation sites HCV the protein N-glycosylation sites Cterm Site HCV sites Point mutations were introduced in the protein N-glycosylation sites of HCV (genotype 1b) and the mutant proteins were analyzed using baculovirus expression system in mammalian and insect cells. 27302155 4 9 gly glycopeptide 728:739 arg2 the Asn144-containing glycopeptide the Asn144-containing glycopeptide glycopeptide Six non-fucosylated diantennary complex type glycoforms were detected on the Asn144-containing glycopeptide. 27302155 4 17 gly detected 694:701 arg1 the Asn144-containing glycopeptide AND Six non-fucosylated diantennary complex type glycoforms the Asn144-containing glycopeptide Six non-fucosylated diantennary complex type glycoforms glycopeptide Six non-fucosylated diantennary complex type glycoforms were detected on the Asn144-containing glycopeptide. 27302155 4 78 gly non-fucosylated 637:651 arg1 Six non-fucosylated diantennary complex type glycoforms Six non-fucosylated diantennary complex type glycoforms Six non-fucosylated diantennary complex type glycoforms were detected on the Asn144-containing glycopeptide. 27302155 4 74 gly glycoforms 678:687 arg1 Six non-fucosylated diantennary complex type glycoforms Six non-fucosylated diantennary complex type glycoforms Six non-fucosylated diantennary complex type glycoforms were detected on the Asn144-containing glycopeptide. 24796651 2 85 gly glycopeptide 451:462 arg2 glycopeptide identification glycopeptide identification glycopeptide Although impressive numbers of glycopeptide identification by a direct database search have been reported, false positives remained high and difficult to determine. 29180407 5 38 gly glycosylated 995:1006 arg1 glycosylated MDR1 and polymeric glucose nanofilaments glycosylated MDR1 and polymeric glucose nanofilaments Nanoscale imaging using immunoelectron microscopy and componential analysis demonstrated that MDR1 is involved in MD ring formation and that the MD ring filaments are composed of glycosylated MDR1 and polymeric glucose nanofilaments. 26764011 4 19 gly 500O-glycoproteins 546:563 arg1 500O-glycoproteins 500O-glycoproteins Fterm 500O-glycoproteins Taking advantage of anO-glycan elongation deficient yeast strain to simplify sample complexity, we identified over 500O-glycoproteins from all subcellular compartments for which over 2300O-mannosylation sites were mapped by electron-transfer dissociation (ETD)-based MS/MS. 26353993 3 18 gly glycoprotein 732:743 arg1 intact glycoprotein intact glycoprotein Fterm glycoprotein Here, we demonstrate that ECD and CID techniques can be used together as an effective top- down method for the structural characterization of intact glycoprotein. 25378534 8 39 part_of FcRn 1502:1505 arg1 the FcRn binding site FcRn the FcRn binding site PUBTATOR Site FcRn 2217 site Removal of antibody glycans increased the flexibility of the FcRn binding site in the Fc region. 25378534 8 91 part_of Fc 1527:1528 arg1 the Fc region Fc the Fc region Cterm Site Fc region Removal of antibody glycans increased the flexibility of the FcRn binding site in the Fc region. 28444931 4 48 gly glycoforms 433:442 arg1 six peptides six peptides peptides Retention behavior of several glycoforms of six peptides obtained from tryptic digest of haptoglobin, hemopexin, and sex hormone-binding globulin was studied on a reversed phase chromatographic column. 24531467 2 33 part_of IgE-binding 411:421 arg1 a potentially important IgE-binding epitope IgE a potentially important IgE-binding epitope PUBTATOR Site IgE P01854 epitope The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope that could be responsible for its cross-reactivity. 29120804 0 66 part_of Impact 125:130 arg1 composition Impact composition OGER Site Impact Q9P2X3 position Comparison of consecutive harvests versus blending treatments to produce lower alcohol wines from Cabernet Sauvignon grapes: Impact on polysaccharide and tannin content and composition. 27294781 1 1 part_of BoNT/A1 305:311 arg1 the BoNT/A1 receptor-binding domain BoNT/A1 the BoNT/A1 receptor-binding domain Cterm Site BoNT/A1 domain Here we report a 2.0-Å-resolution crystal structure of the BoNT/A1 receptor-binding domain in complex with its neuronal receptor, glycosylated human SV2C. 27294781 1 3 part_of receptor-binding 313:328 arg1 the BoNT/A1 receptor-binding domain receptor the BoNT/A1 receptor-binding domain Fterm Site receptor domain Here we report a 2.0-Å-resolution crystal structure of the BoNT/A1 receptor-binding domain in complex with its neuronal receptor, glycosylated human SV2C. 29023123 5 13 gly galactose 1024:1032 arg1 chemical composition chemical composition chemical composition Site position Furthermore, the bile acid-binding capacity of LP-A8 was obviously higher than the other fractions, which may be attributed to its highly branched structure, abundant sulfate, fucose, and galactose in chemical composition and denser interconnected macromolecule network in molecular morphology. 29023123 5 17 gly fucose 1012:1017 arg1 chemical composition chemical composition chemical composition Site position Furthermore, the bile acid-binding capacity of LP-A8 was obviously higher than the other fractions, which may be attributed to its highly branched structure, abundant sulfate, fucose, and galactose in chemical composition and denser interconnected macromolecule network in molecular morphology. 29130773 9 8 gly glycoproteins 1065:1077 arg1 these glycoproteins these glycoproteins Fterm glycoproteins Meanwhile, these glycoproteins also exhibited significant age-associated alterations. 25505447 1 63 gly serine 131:136 arg1 The carbohydrate modification serine and threonine residues The carbohydrate modification serine and threonine residues The carbohydrate modification of serine and threonine residues with O-linked beta- N-acetylglucosamine (O-GlcNAc) is ubiquitous and governs cellular processes ranging from cell signaling to apoptosis. 25505447 1 76 gly threonine 142:150 arg1 The carbohydrate modification serine and threonine residues The carbohydrate modification serine and threonine residues The carbohydrate modification of serine and threonine residues with O-linked beta- N-acetylglucosamine (O-GlcNAc) is ubiquitous and governs cellular processes ranging from cell signaling to apoptosis. 28473830 1 39 gly glycoprotein 142:153 arg1 Hemagglutinin glycoprotein Hemagglutinin glycoprotein Fterm glycoprotein Hemagglutinin glycoprotein (HA) is a principle influenza vaccine antigen. 29130773 3 22 gly proteins 305:312 arg1 the glycans proteins the glycans Fterm proteins Structural changes in the glycans of saliva proteins might be reliable indicators of OLP. 24531467 8 63 part_of IgE 1379:1381 arg1 an allergenic IgE epitope IgE an allergenic IgE epitope PUBTATOR Site IgE P01854 epitope Immunological studies suggested that carbohydrates on Hev b 2 represent an allergenic IgE epitope. 25092905 6 43 gly O-glycoproteins 1232:1246 arg1 O-glycoproteins O-glycoproteins Fterm O-glycoproteins Here, we have genetically engineered CHO cells to produce homogeneous truncated O-glycans, so-called SimpleCells, which enabled lectin enrichment of O-glycoproteins and characterization of the O-glycoproteome. 29150937 8 12 gly glycoprotein 1491:1502 arg1 such a complex glycoprotein such a complex glycoprotein Fterm glycoprotein The final cGMP production run yielded 3.52 g (peptidic mass) of fully purified trimers (Drug Substance) from a 200 L bioreactor, a notable yield for such a complex glycoprotein. 29150937 4 98 gly glycosylated 781:792 arg1 BG505 SOSIP.664 trimers BG505 SOSIP.664 trimers Fterm trimers BG505 SOSIP.664 trimers are extensively glycosylated, contain numerous disulfide bonds and require proteolytic cleavage, all properties that pose a substantial challenge to cGMP production. 24085812 14 47 gly fucosylated 2097:2107 arg1 PON1 PON1 their fucosylation levels PUBTATOR PON1 5444 Our data suggest that fucosylated protein biomarkers, such as PON1, and their fucosylation levels and patterns can serve as diagnostic and prognostic serological markers for SCLC. 25862865 6 6 gly integrin 828:835 arg1 the sialylation integrin the sialylation Fterm integrin We also observed a shift in the sialylation of αvβ3 integrin related to reduction of α2-6-linked sialic acid expression and an increase of α2-3 sialylation of both subunits in melanoma progression. 25862865 6 15 gly sialylation 920:930 arg1 both subunits both subunits Fterm subunits We also observed a shift in the sialylation of αvβ3 integrin related to reduction of α2-6-linked sialic acid expression and an increase of α2-3 sialylation of both subunits in melanoma progression. 25862865 6 59 gly sialylation 808:818 arg1 αvβ3 integrin αvβ3 integrin Fterm integrin We also observed a shift in the sialylation of αvβ3 integrin related to reduction of α2-6-linked sialic acid expression and an increase of α2-3 sialylation of both subunits in melanoma progression. 25862865 6 62 gly subunits 940:947 arg1 α2-3 sialylation subunits α2-3 sialylation Fterm subunits We also observed a shift in the sialylation of αvβ3 integrin related to reduction of α2-6-linked sialic acid expression and an increase of α2-3 sialylation of both subunits in melanoma progression. 24769397 4 12 gly glycosylated 656:667 arg1 synthetic glycans proteins synthetic glycans Fterm proteins METHODS In this study, biochemical properties of calmegin and calnexin were compared using synthetic glycans and glycosylated or non-glycosylated proteins as substrates. 24769397 4 22 gly non-glycosylated 672:687 arg1 synthetic glycans proteins synthetic glycans Fterm proteins METHODS In this study, biochemical properties of calmegin and calnexin were compared using synthetic glycans and glycosylated or non-glycosylated proteins as substrates. 28332009 3 17 gly epitopes 830:837 arg1 the plant epitopes the plant epitopes epitopes The stable co-expression of a human β-1,4-galactosyltransferase targeted to different Golgi sub-compartments altered Lo-BM2N-glycosylation and resulted in the production of an antibody that exhibited either hybrid structures containing a low abundance of the plant epitopes (α-1,3-fucose and β-1,2-xylose), or a large amount of galactose-extended N-glycan structures. 29046357 5 5 gly glycopeptide 780:791 arg2 glycopeptide glycopeptide glycopeptide However, the contribution of each glycopeptide component to PILRα binding was largely unclear. 27413183 3 64 part_of site 575:578 arg1 calreticulin calreticulin site PUBTATOR Site calreticulin 811 site Although the lectin-oligosaccharide association is well understood, the polypeptide-based interaction is more controversial because the binding site on calreticulin has not been identified, and its significance in the biogenesis of glycoproteins in cells remains unknown. 26169738 6 45 gly glycoproteins 1075:1087 arg1 the membrane glycoproteins hemagglutinin and neuraminidase the membrane glycoproteins hemagglutinin and neuraminidase Fterm glycoproteins The protocol is exemplarily demonstrated for N-glycosylation fingerprinting of cell culture-derived influenza A and B viruses and their major antigens, the membrane glycoproteins hemagglutinin and neuraminidase. 26169738 6 45 gly glycoproteins 1075:1087 arg1 hemagglutinin hemagglutinin Fterm hemagglutinin The protocol is exemplarily demonstrated for N-glycosylation fingerprinting of cell culture-derived influenza A and B viruses and their major antigens, the membrane glycoproteins hemagglutinin and neuraminidase. 29050610 6 13 part_of BNC/CH-Hep 1038:1047 arg1 the BNC/CH-Hep composites Hep the BNC/CH-Hep composites OGER Site Hep Q9Y251 composites The EDC/NHS crosslinking catalyzed both amide bonds and ester bonds formation in the BNC/CH-Hep composites. 24945804 1 27 part_of hemagglutinin 148:160 arg1 the globular domain hemagglutinin the globular domain Fterm Site hemagglutinin domain Antigenic variation in the globular domain of influenza A virus (IAV) hemagglutinin (HA) precludes effective immunity to this major human pathogen. 25092905 3 3 gly O-glycosylation 545:559 arg1 proteins proteins Fterm proteins We have previously shown that the capacity for O-glycosylation of proteins can be one limiting parameter for production of active proteins in CHO. 23934913 6 47 gly N-glycosylation 818:832 arg2 the structural and sequentially conserved N-glycosylation sites the structural and sequentially conserved N-glycosylation sites sites and lysines We identified candidate lysines by analyzing the structural and sequentially conserved N-glycosylation sites and lysines in bPLBD1 and in the homologous mouse PLBD2. 25548871 13 43 gly used 1894:1897 arg2 this novel composite this novel composite composite Furthermore, the fact that the [CEL+CS+KER] composite has combined properties of its components, namely, superior mechanical strength (CEL), hemostasis and bactericide (CS), and controlled drug release (KER), indicates that this novel composite can be used in ways which hitherto were not possible, e.g., as a high-performance bandage to treat chronic and ulcerous wounds. 25594223 3 36 gly glycosylation 346:358 arg2 varying glycosylation sites varying glycosylation sites sites A set of glycosylated ENFs with varying glycosylation sites and glycan structures were synthesized. 24943676 4 26 gly glycosylation 887:899 arg1 the S-layer proteins the S-layer proteins Fterm proteins To investigate, if T9SS is functional in T. forsythia, T9SS-deficient mutants were generated by targeting either TF0955 (putative C-terminal signal peptidase) or TF2327 (PorK ortholog), and the mutants were analyzed with respect to secretion, assembly and glycosylation of the S-layer proteins as well as proteolytic processing of the CTD and biofilm formation. 28064042 8 4 gly glycoproteins 1228:1240 arg1 glycoproteins glycoproteins Fterm glycoproteins Thus, the reactivity for N-linked oligosaccharide processing of glycoproteins in the endoplasmic reticulum might be tunable by the aglycone structure, e.g., protein portion of glycoproteins. 28064042 8 42 gly glycoproteins 1116:1128 arg1 N-linked oligosaccharide processing glycoproteins N-linked oligosaccharide processing Fterm glycoproteins Thus, the reactivity for N-linked oligosaccharide processing of glycoproteins in the endoplasmic reticulum might be tunable by the aglycone structure, e.g., protein portion of glycoproteins. 24526361 8 49 gly glycoproteins 1576:1588 arg1 intact glycoproteins intact glycoproteins Fterm glycoproteins As such, this presents for the first time a strategy to remove O-linked glycosylation from intact glycoproteins expressed in P. pastoris. 25210975 1 27 gly glycoproteins 263:275 arg1 biologically active glycoproteins biologically active glycoproteins Fterm glycoproteins The secreted cellular sub-proteome (secretome) is a rich source of biologically active glycoproteins. 28290279 5 78 part_of lignin 593:598 arg1 lignin contents/composition lignin lignin contents/composition Fterm Site lignin position Effects on dietary fiber contents, polysaccharide composition, and on lignin contents/composition were much more pronounced during storage at 20°C than at 1°C. 29150937 11 22 gly glycoprotein 1935:1946 arg1 other native-like Env glycoprotein trimers other native-like Env glycoprotein trimers Fterm glycoprotein The methods reported here should pave the way for the cGMP production of other native-like Env glycoprotein trimers of various designs and genotypes. 28731703 2 0 part_of glycyrrhizin 518:529 arg1 the solid-state nanodispersed compositions glycyrrhizin the solid-state nanodispersed compositions Fterm Site glycyrrhizin positions The mechanochemical technique has been applied to prepare the solid-state nanodispersed compositions of antidote 1,8-naphthalic anhydride (NA) with arabinogalactan, sodium salt of carboxymethylcellulose, and glycyrrhizin as DSs. 28054967 2 33 part_of G1 493:494 arg1 the fragment crystallizable (Fc) domain immunoglobulin G1 the fragment crystallizable (Fc) domain Cterm Site immunoglobulin G1 domain In this study, we produced a secreted anthrax decoy fusion protein comprised of a portion of the human capillary morphogenesis gene-2 (CMG2) protein fused via a linker to the fragment crystallizable (Fc) domain of human immunoglobulin G1 in Nicotiana benthamiana plants using a transient expression system. 27440889 4 126 gly glycosylation 911:923 arg2 deleted N-linked glycosylation sites deleted N-linked glycosylation sites sites Since the HA stem region is constituted by an HA1 N-terminal part and a full HA2 part, we expressed a series of recombinant HA mutant proteins with deleted N-linked glycosylation sites in the HA1 stem and HA2 stem regions of H5N1 and pH1N1 viruses. 28877283 2 9 part_of Light 281:285 arg1 Light Bond composite Light Light Bond composite OGER Site Light O43557 composite MATERIAL AND METHODS Forty specimens were fabricated from Transbond XT and Light Bond composite and bonding agent components (n=10/component), then contaminated by immersion in a bacterial endotoxin solution. 26641950 11 18 gly glycoprotein 1744:1755 arg1 intact glycoprotein species intact glycoprotein species Fterm glycoprotein We demonstrate that high resolution CZE separation of intact glycoprotein species coupled to MS has significant potential for the in-depth characterization and quantitative analysis of biopharmaceutical proteoforms. 26512888 5 16 gly glycoprotein 692:703 arg1 one CTB-binding glycoprotein one CTB-binding glycoprotein Fterm glycoprotein Using a metabolically incorporated photocrosslinking sugar, we identified one CTB-binding glycoprotein and demonstrated that the glycan portion of the molecule, not the protein, provides the CTB interaction motif. 24618259 5 29 gly sialylated 902:911 arg1 sialylated N-glycans sialylated N-glycans In particular, we show here that co-expression of BChE with a novel gene-stacking vector, carrying six mammalian genes necessary for in planta protein sialylation, resulted in the generation of rBChE decorated with sialylated N-glycans. 27313224 4 81 gly N-glycosylation 774:788 arg2 five putative N-glycosylation sites sites sites SV2C-LD4 exhibits the strongest protein-protein interaction and comprises five putative N-glycosylation sites (PNG sites). 29416597 7 31 gly glycosylation 1043:1055 arg2 glycosylation sites glycosylation sites sites These results indicated that different locations of glycosylation sites could lead to diverse presentation of peptides and glycopeptides to APCs and influence the immunogenicity of glycoconjugate vaccine, which extend the current understanding of mechanism for adaptive immune system activation by glycoconjugate vaccine, and have implications for rational glycoconjugate vaccine design. 29416597 7 49 gly glycopeptides 1114:1126 arg2 glycopeptides glycopeptides glycopeptides These results indicated that different locations of glycosylation sites could lead to diverse presentation of peptides and glycopeptides to APCs and influence the immunogenicity of glycoconjugate vaccine, which extend the current understanding of mechanism for adaptive immune system activation by glycoconjugate vaccine, and have implications for rational glycoconjugate vaccine design. 27294781 0 39 gly glycosylation 9:21 arg1 botulinum neurotoxin botulinum neurotoxin Fterm neurotoxin A N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A. Botulinum neurotoxin serotype A1 (BoNT/A1), a licensed drug widely used for medical and cosmetic applications, exerts its action by invading motoneurons. 24618259 3 17 gly sialylated 445:454 arg1 a highly sialylated, tetrameric serum protein a highly sialylated, tetrameric serum protein Fterm protein Human butyrylcholinesterase (BChE) is a highly sialylated, tetrameric serum protein, investigated as a bioscavenger for organophosphorous nerve agents. 27511022 5 40 gly glycosylation 871:883 arg2 all presumptive sites all presumptive sites sites To check this possibility, we mutated all presumptive sites of glycosylation and tested the properties of the resulting modified Hsp70 expressed in E. coli. 27716795 0 35 part_of Region 45:50 arg1 the V1V2 Variable Domain Domain Region Fterm Domain 155971 Effect of Glycosylation on an Immunodominant Region in the V1V2 Variable Domain of the HIV-1 Envelope gp120 Protein. 27716795 0 35 part_of Region 45:50 arg1 the HIV-1 Envelope gp120 Protein gp120 Protein Region PUBTATOR gp120 Protein 155971 Effect of Glycosylation on an Immunodominant Region in the V1V2 Variable Domain of the HIV-1 Envelope gp120 Protein. 27716795 0 67 part_of Protein 108:114 arg1 the V1V2 Variable Domain gp120 Protein the V1V2 Variable Domain PUBTATOR gp120 Protein 155971 Effect of Glycosylation on an Immunodominant Region in the V1V2 Variable Domain of the HIV-1 Envelope gp120 Protein. 25422905 3 27 gly non-glycosylated 575:590 arg1 a non-glycosylated protein a non-glycosylated protein Fterm protein We have expressed Gd (carrying two amino acid substitutions to improve solubility) as a non-glycosylated protein in Escherichia coli via periplasmic secretion and determined its X-ray structure at 2.45 Å resolution. 24753400 6 25 gly glycopeptide 1078:1089 arg2 the glycopeptide antigen the glycopeptide antigen glycopeptide Further evaluation by ELISA neutralization experiments and glycopeptide microarrays showed that the induced antibodies were highly specific to the glycopeptide antigen. 24753400 6 48 gly glycopeptide 990:1001 arg2 glycopeptide microarrays glycopeptide microarrays glycopeptide Further evaluation by ELISA neutralization experiments and glycopeptide microarrays showed that the induced antibodies were highly specific to the glycopeptide antigen. 25271059 7 56 gly branching 1090:1098 arg1 the nascent protein protein branching Fterm protein We identified a mechanism in which galactosyltransferase 4 isoform regulated N-glycan branching on the nascent protein, subsequently controlling biological activity in an in vivo model of hCG activity. 26689158 7 0 gly derived 1014:1020 arg2 chain termini AND two disaccharides chain termini two disaccharides termini These included eight common disaccharides, two disaccharides derived from chain termini, three 3-O-sulfo-group-containing tetrasaccharides, along with three linkage region tetrasaccharides and their derivatives. 26689158 7 73 gly region 1118:1123 arg1 three linkage region tetrasaccharides three linkage region tetrasaccharides These included eight common disaccharides, two disaccharides derived from chain termini, three 3-O-sulfo-group-containing tetrasaccharides, along with three linkage region tetrasaccharides and their derivatives. 27130732 7 32 part_of having 1148:1153 arg1 ECM proteins AND LG domains ECM proteins LG domains OGER Site ECM proteins Q13201 domains Thus, we propose a novel structure-a ribitol in a phosphodiester linkage-for the moiety on which TMEM5, B4GAT1, and LARGE act to generate the functional receptor for ECM proteins having LG domains. 24585882 9 28 part_of motifs 1198:1203 arg1 the Pbx proteins proteins motifs Fterm Site proteins motifs These results, together with the sequence motifs in the Pbx proteins, suggest that Pbx1 and Pbx2 are redundant proteins that act in remodeling the cell wall to maintain normal cell morphology and precursor availability for other glycan synthetic processes. 27241276 1 8 gly asparagine-linked 232:248 arg1 asparagine-linked GlcNAc asparagine asparagine-linked GlcNAc asparagine In plants, α1,3-fucosyltransferase (FucT) catalyzes the transfer of fucose from GDP-fucose to asparagine-linked GlcNAc of the N-glycan core in the medial Golgi. 25151388 1 2 gly glycoproteins 157:169 arg1 glycoproteins glycoproteins Fterm glycoproteins Protein N-glycosylation can influence the nervous system in a variety of ways by affecting functions of glycoproteins involved in nervous system development and physiology. 27034286 6 122 gly glycosylation 936:948 arg1 these serum proteins these serum proteins Fterm proteins The changes in glycosylation pattern of these serum proteins were assayed using different lectins. 26587964 4 21 gly having 737:742 arg1 a new glycosyl crambin AND complex-type oligosaccharide a new glycosyl crambin complex-type oligosaccharide Fterm crambin For this purpose, we synthesized a new glycosyl crambin having complex-type oligosaccharide and evaluated the folding process, the final protein structure analyzed by NMR, and compared the CD spectra with previously synthesized glycosyl crambin bearing high mannose-type oligosaccharides. 25885924 1 18 gly glycoprotein 181:192 arg1 a glycoprotein a glycoprotein Fterm glycoprotein Neural cell adhesion molecule 140 (NCAM-140) is a glycoprotein and always highly polysialylated in cancer. 24574058 1 54 gly strain 239:244 arg1 N-glycans strain N-glycans Fterm strain In this study, we have performed the first mass spectrometric analysis of N-glycans of the M31 mutant strain of the cellular slime mould Dictyostelium discoideum, previously shown to have a defect in glucosidase II. 25964011 6 80 gly N-glycopeptide 1060:1073 arg2 the asparagine-rich N-glycopeptide [34-45] FESNFNTQATNR the asparagine-rich N-glycopeptide [34-45] FESNFNTQATNR N-glycopeptide A novel data interpretation workflow based on MS/MS spectra classification and glycan database searching enabled the straightforward identification of the asparagine-rich N-glycopeptide [34-45] FESNFNTQATNR and allowed for compositional profiling of its modifying N-glycans. 29292146 7 64 part_of contains 1212:1219 arg1 A possible third domain AND contiguous 2-Rhap residues A possible third domain contiguous 2-Rhap residues residues A possible third domain contains contiguous 2-Rhap residues, some branched at O-3. 25000122 5 23 gly O-glycosylation 759:773 arg2 five O-glycosylation sites five O-glycosylation sites sites We prepared two recombinant OPNs, WT (wild-type)-OPN and mutant OPN (ΔO-OPN), which lacks five O-glycosylation sites at a threonine/proline-rich region. 28656506 5 5 gly monoglycosylated 866:881 arg1 monoglycosylated drosocin monoglycosylated drosocin Fterm drosocin The disaccharide containing drosocin exhibited lower potency compared to monoglycosylated drosocin against all the tested Gram negative bacteria, suggesting the role of the distal sugar or increase in the sugar chain length on the activity. 29247593 7 51 gly monosialylated 1065:1078 arg1 monosialylated and disialylated forms monosialylated and disialylated forms By this method, >90% of a disialylated human-llama antibody (EG2-hFc) and equimolar quantities of monosialylated and disialylated forms of human antibodies (αIL8-hFc and human polyclonal) are produced. 27222530 3 54 gly glycosylated 669:680 arg1 glycosylated receptors glycosylated receptors Fterm receptors Such equilibrium critically governs the function of this lectin signaling by allowing tunable interactions with a preferential set of glycosylated receptors. 27085741 4 94 gly glycopeptides 998:1010 arg2 glycopeptides glycopeptides glycopeptides The hydrophilic interaction liquid chromatography (HILIC) was shown as an efficient technique for separation of labelled or native neutral and acidic glycans, glycopeptides, sialylated glycans, glycosylated and nonglycosylated peptides. 27085741 4 100 gly sialylated 1013:1022 arg1 labelled or native neutral and acidic glycans labelled or native neutral and acidic glycans The hydrophilic interaction liquid chromatography (HILIC) was shown as an efficient technique for separation of labelled or native neutral and acidic glycans, glycopeptides, sialylated glycans, glycosylated and nonglycosylated peptides. 27085741 4 100 gly sialylated 1013:1022 arg1 sialylated glycans sialylated glycans The hydrophilic interaction liquid chromatography (HILIC) was shown as an efficient technique for separation of labelled or native neutral and acidic glycans, glycopeptides, sialylated glycans, glycosylated and nonglycosylated peptides. 27085741 4 67 gly glycosylated 1033:1044 arg1 glycosylated and nonglycosylated peptides peptides labelled or native neutral and acidic glycans peptides The hydrophilic interaction liquid chromatography (HILIC) was shown as an efficient technique for separation of labelled or native neutral and acidic glycans, glycopeptides, sialylated glycans, glycosylated and nonglycosylated peptides. 27085741 4 72 gly nonglycosylated 1050:1064 arg1 glycosylated and nonglycosylated peptides peptides labelled or native neutral and acidic glycans peptides The hydrophilic interaction liquid chromatography (HILIC) was shown as an efficient technique for separation of labelled or native neutral and acidic glycans, glycopeptides, sialylated glycans, glycosylated and nonglycosylated peptides. 26261057 8 76 gly N-glycosylated 1443:1456 arg1 the N-glycosylated peptide the N-glycosylated peptide peptide The mass spectra of the N-glycosylated peptide revealed that the observed biological properties were attributable to the characteristic N-glycan structures of the anti-CD20 mAbs produced in the transgenic silkworms, i.e., the lack of the core-fucose and galactose at the non-reducing terminal. 26857855 8 74 part_of enzyme 1504:1509 arg1 the amino acid sequence enzyme the amino acid sequence Fterm Site enzyme sequence To our knowledge, this is the first report describing the amino acid sequence and structural features of a heat-resistant FOS-forming enzyme from A. aculeatus, providing insights into its potential applications in the prebiotics industry. 27716795 9 0 part_of gp120 1874:1878 arg1 the variable domains gp120 the variable domains PUBTATOR Site gp120 Q14624 domains Together, these results provide a mechanism for conservation of disulfide linkage proximal glycosylation adjacent to the variable domains of gp120 and begin to explain how this could be exploited to enhance the immunogenicity of those regions. 26235297 3 30 gly glycosylated 567:578 arg1 the common acceptor Kdoα(2-4)Kdo the common acceptor Kdoα(2-4)Kdo To obtain 4,5-branched core oligosaccharide structures, the common acceptor Kdoα(2-4)Kdo was glycosylated with the Hep units. 25187293 1 60 part_of glucocerebrosidase 183:200 arg1 vacuolar anchoring domains glucocerebrosidase vacuolar anchoring domains Fterm Site glucocerebrosidase domains Human glucocerebrosidase with vacuolar anchoring domains was targeted to protein storage vacuoles (PSVs) of Arabidopsis seeds, but unexpectedly via the Golgi complex. 26721331 1 2 gly sialylation 165:175 arg1 glycans glycans BACKGROUND Terminal α2-3 and α2-6 sialylation of glycans precludes further chain elongation, leading to the biosynthesis of cancer relevant epitopes such as sialyl-Lewis X (SLe(X)). 28743910 4 8 part_of protein 661:667 arg1 This protein sequence protein This protein sequence Fterm Site protein sequence This protein sequence contains 432 amino acids with the presence of three structurally different domains: an N-terminal signal peptide, a C-terminal LPXTG motif, and a PT repeat region. 26618514 1 29 gly glycosylation 204:216 arg2 a single glycosylation site a single glycosylation site site The Fc portion of immunoglobulin (Ig)G harbours a single glycosylation site. 27031228 5 51 gly anti-glycopeptide 799:815 arg2 anti-glycopeptide anti-glycopeptide anti-glycopeptide Herein, we report the development of a novel anti-glycopeptide mAb (GpMab), LpMab-12. 24366264 1 15 gly channels 306:313 arg1 the lipopolysaccharide (LPS)-dependent modulation channels the lipopolysaccharide (LPS)-dependent modulation Fterm channels Depressed heart rate variability in severe inflammatory diseases can be partially explained by the lipopolysaccharide (LPS)-dependent modulation of cardiac pacemaker channels. 28826211 2 70 gly glycoproteins 259:271 arg1 glycoproteins glycoproteins Fterm glycoproteins Because of the low abundance of glycoproteins and high complexity of clinical samples, the development of methods to selectively capture glycoproteins/glycopeptides is crucial to glycoproteomics study. 28826211 2 99 gly glycoproteins/glycopeptides 364:390 arg2 glycoproteins/glycopeptides glycoproteins/glycopeptides glycoproteins/glycopeptides Because of the low abundance of glycoproteins and high complexity of clinical samples, the development of methods to selectively capture glycoproteins/glycopeptides is crucial to glycoproteomics study. 26565759 3 73 gly glycopeptide 595:606 arg2 glycopeptide compositions glycopeptide compositions glycopeptide We have developed modular, high-throughput data processing software, MassyTools, that is capable of calibrating spectra, extracting data, and performing quality control calculations based on a user-defined list of glycan or glycopeptide compositions. 25187293 4 36 part_of protein 895:901 arg1 the BP-80 transmembrane domain protein the BP-80 transmembrane domain Fterm Site protein domain A reporter protein fused to a vacuolar membrane targeting motif comprised of the BP-80 transmembrane domain (TMD), and the cytoplasmic tail (CT) of α-tonoplast intrinsic protein (α-TIP) is delivered to protein storage vacuoles (PSVs) of tobacco seeds by ER-derived transport vesicles that bypass the Golgi complex. 25187293 4 36 part_of protein 895:901 arg1 the cytoplasmic tail protein the cytoplasmic tail Fterm Site protein tail A reporter protein fused to a vacuolar membrane targeting motif comprised of the BP-80 transmembrane domain (TMD), and the cytoplasmic tail (CT) of α-tonoplast intrinsic protein (α-TIP) is delivered to protein storage vacuoles (PSVs) of tobacco seeds by ER-derived transport vesicles that bypass the Golgi complex. 27031228 10 16 gly non-sialylated 1346:1359 arg1 non-sialylated GalNAc-attached glycopeptide non-sialylated GalNAc-attached glycopeptide LpMab-12 did not recognize non-sialylated GalNAc-attached glycopeptide, indicating that sialylated GalNAc on Thr52 is necessary for the binding of LpMab-12 to hPDPN. 27031228 10 64 gly sialylated 1407:1416 arg1 sialylated GalNAc sialylated GalNAc LpMab-12 did not recognize non-sialylated GalNAc-attached glycopeptide, indicating that sialylated GalNAc on Thr52 is necessary for the binding of LpMab-12 to hPDPN. 25766612 0 54 gly N-Glycosylation 108:122 arg1 the Sequon Asn Asn(204) Asn(204) Association between RABV G Proteins Transported from the Perinuclear Space to the Cell Surface Membrane and N-Glycosylation of the Sequon Asn(204). 28407380 0 82 gly glycoprotein 69:80 arg1 basic and applied glycoprotein research basic and applied glycoprotein research Fterm glycoprotein N-glycan maturation mutants in Lotus japonicus for basic and applied glycoprotein research. 26076608 7 41 gly compositions 1086:1097 arg1 the different mulberry fruit polysaccharides fractions positions the different mulberry fruit polysaccharides fractions positions This may be caused by their different compositions and physical properties in the different mulberry fruit polysaccharides fractions. 28064042 1 2 gly glycoprotein 193:204 arg1 glycoprotein folding glycoprotein folding Fterm glycoprotein Glycoprotein N-linked oligosaccharides in the endoplasmic reticulum function as tags to regulate glycoprotein folding, sorting, secretion and degradation. 24678018 6 25 gly N56 1012:1014 arg1 Fifteen structurally related N-glycans N29 and N56 Fifteen structurally related N-glycans N29 and N56 Fifteen structurally related N-glycans at N29 and N56 were observed, with different N-glycan compositional variants being preferred on each amino acid. 24678018 6 32 gly N29 1004:1006 arg1 Fifteen structurally related N-glycans N29 and N56 Fifteen structurally related N-glycans N29 and N56 Fifteen structurally related N-glycans at N29 and N56 were observed, with different N-glycan compositional variants being preferred on each amino acid. 25117255 1 15 gly glycosylation 130:142 arg1 proteins proteins Fterm proteins To consider biological significance of glycosylation of proteins, it is necessary to evaluate the importance of sugar-recognition processes mediated by lectins. 26655437 5 58 gly fucosylation 605:616 arg1 placental glycans placental glycans Results have shown that preeclampsia reduced fucosylation of placental glycans, increased the appearance of paucimannosidic and mannosidic structures with lower number of mannose residues and decreased the amount of glycans with more mannose residues. 27796371 4 48 gly glycosylation 671:683 arg1 receptor binding receptor binding Fterm receptor We evaluated the effect of hemagglutinin glycosylation on receptor binding and virulence of engineered H3N2 viruses. 25609749 3 1 gly glycoproteins 684:696 arg1 serum glycoproteins serum glycoproteins Fterm glycoproteins TMEM165-CDG patients exhibit cartilage and bone dysplasia and altered glycosylation of serum glycoproteins. 25609749 3 12 gly glycosylation 661:673 arg1 serum glycoproteins serum glycoproteins Fterm glycoproteins TMEM165-CDG patients exhibit cartilage and bone dysplasia and altered glycosylation of serum glycoproteins. 27440889 8 98 gly proteins 1626:1633 arg1 the HA2 stem N-glycans proteins the HA2 stem N-glycans Fterm proteins Unmasking the HA2 stem N-glycans of H5HA mutant proteins showed a significantly improvement in the protection against homologous virus challenges but did so to a less degree for the protection against heterosubtypic pH1N1 virus challenges. 26424213 4 77 gly monoglycosylated 864:879 arg1 β-D-glucosyl-drosocin β-D-glucosyl-drosocin Fterm β-D-glucosyl-drosocin The structural and functional properties of these disaccharide-containing formaecin I and drosocin analogs were compared with their corresponding monoglycosylated forms, β-D-glucosyl-formaecin I and β-D-glucosyl-drosocin, respectively. 25263894 1 8 gly residue 165:171 arg1 A novel water-soluble polysaccharide residue A novel water-soluble polysaccharide residue A novel water-soluble polysaccharide from Pleurotus eryngii residue was isolated and further purified by DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography to yield PEPE-1, PEPE-2 and PEPE-3. 24085812 6 22 gly glycoproteins 892:904 arg1 the fucosylated glycoproteins the fucosylated glycoproteins Fterm glycoproteins In a narrowed down and comparative data analysis of both label-free proteomics and isobaric peptide-tagging chemistry iTRAQ approaches, the fucosylated glycoproteins were identified as up- or down-regulated in the sera of limited disease and extensive disease stage patients with SCLC. 24085812 6 87 gly fucosylated 880:890 arg1 the fucosylated glycoproteins the fucosylated glycoproteins Fterm glycoproteins In a narrowed down and comparative data analysis of both label-free proteomics and isobaric peptide-tagging chemistry iTRAQ approaches, the fucosylated glycoproteins were identified as up- or down-regulated in the sera of limited disease and extensive disease stage patients with SCLC. 24945804 0 33 part_of hemagglutinin 32:44 arg1 influenza a virus hemagglutinin cross-protective stem epitopes hemagglutinin influenza a virus hemagglutinin cross-protective stem epitopes Fterm Site hemagglutinin epitopes Biogenesis of influenza a virus hemagglutinin cross-protective stem epitopes. 24526361 0 66 gly glycoproteins 68:80 arg1 intact glycoproteins glycoproteins O-linked mannose Fterm glycoproteins In vitro enzymatic treatment to remove O-linked mannose from intact glycoproteins. 27077895 4 0 gly region 798:803 arg1 internal region reducing oligosaccharides internal region reducing oligosaccharides In the first, intact W-sol AXs are treated with 2AB to tag the original RE backbone chain sugar residue and then treated with an endoxylanase to generate a mixture of 2AB-labelled RE and internal region reducing oligosaccharides, respectively. 27413183 7 63 gly glycoproteins 1617:1629 arg1 newly synthesized glycoproteins newly synthesized glycoproteins Fterm glycoproteins To assess the relative contributions of the glycan-dependent and -independent interactions in living cells, we expressed lectin-deficient, polypeptide binding-deficient, and doubly deficient calreticulin constructs in calreticulin-negative cells and monitored the effects on the biogenesis of MHC class I molecules, the solubility of mutant forms of α1-antitrypsin, and interactions with newly synthesized glycoproteins. 24085812 8 107 gly fucosylated 1136:1146 arg1 Four fucosylated proteins Four fucosylated proteins Fterm proteins Four fucosylated proteins, APCS, C9, SERPINA4, and PON1, were selected and subsequently validated by hybrid A. aurantia lectin ELISA (HLE) and Western blotting. 26559536 4 35 gly sialylated 717:726 arg1 multi-branched and highly sialylated N-glycans multi-branched and highly sialylated N-glycans Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. 26424213 2 88 part_of formaecin 416:424 arg1 proline-rich antimicrobial glycopeptides formaecin I proline-rich antimicrobial glycopeptides Cterm Site formaecin I glycopeptides We have analyzed the effect of distal sugar and interglycosidic linkage of disaccharides on the properties of proline-rich antimicrobial glycopeptides, formaecin I and drosocin. 26641950 3 39 gly glycosylation 711:723 arg1 recombinant human interferon-β1 interferon-β1 site PUBTATOR interferon-β1 3456 site Here, we report on capillary zone electrophoresis (CZE) coupled via a commercial CESI sheathless interface to an Orbitrap ELITE MS for the intact analysis of recombinant human interferon-β1 (Avonex, rhIFN-β1), a biopharmaceutical with complex glycosylation at a single N-linked site. 26641950 3 39 gly glycosylation 711:723 arg1 a single N-linked site site site Here, we report on capillary zone electrophoresis (CZE) coupled via a commercial CESI sheathless interface to an Orbitrap ELITE MS for the intact analysis of recombinant human interferon-β1 (Avonex, rhIFN-β1), a biopharmaceutical with complex glycosylation at a single N-linked site. 26641950 3 39 gly glycosylation 711:723 arg1 a single N-linked site interferon-β1 site PUBTATOR interferon-β1 3456 site Here, we report on capillary zone electrophoresis (CZE) coupled via a commercial CESI sheathless interface to an Orbitrap ELITE MS for the intact analysis of recombinant human interferon-β1 (Avonex, rhIFN-β1), a biopharmaceutical with complex glycosylation at a single N-linked site. 28990779 4 46 gly N-glycopeptides 1045:1059 arg2 complex N-glycopeptides complex N-glycopeptides N-glycopeptides We found that mutation at the nucleophilic residue (D200) did not provide a typical glycosynthase from this bacterial enzyme, but several mutants with mutation at the general acid/base residue E274 of the Lactobacillus casei α1,6-fucosidase, including E274A, E274S, and E274G, acted as efficient glycoligases that could fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using α-fucosyl fluoride as a simple donor substrate. 28990779 4 83 gly glycoproteins 1072:1084 arg1 intact glycoproteins intact glycoproteins Fterm glycoproteins We found that mutation at the nucleophilic residue (D200) did not provide a typical glycosynthase from this bacterial enzyme, but several mutants with mutation at the general acid/base residue E274 of the Lactobacillus casei α1,6-fucosidase, including E274A, E274S, and E274G, acted as efficient glycoligases that could fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using α-fucosyl fluoride as a simple donor substrate. 28990779 4 38 gly fucosylate 1008:1017 arg1 intact glycoproteins glycoproteins N-glycopeptides Fterm glycoproteins N-glycopeptides We found that mutation at the nucleophilic residue (D200) did not provide a typical glycosynthase from this bacterial enzyme, but several mutants with mutation at the general acid/base residue E274 of the Lactobacillus casei α1,6-fucosidase, including E274A, E274S, and E274G, acted as efficient glycoligases that could fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using α-fucosyl fluoride as a simple donor substrate. 27474680 12 57 gly used 2059:2062 arg2 these composites these composites composites These results together with our previous finding that [CEL+KER] composites can control release of drug such as ciprofloxacin clearly indicate that these composites can potentially be used as wound dressing. 25484063 7 48 gly N-glycosylation 1119:1133 arg2 most N-glycosylation sites most N-glycosylation sites sites Glycoanalysis revealed predominantly high mannose structures present on most N-glycosylation sites, with limited evidence for complex glycosylation or processing to paucimannosidic forms. 25484063 7 72 gly present 1103:1109 arg2 most N-glycosylation sites AND high mannose structures most N-glycosylation sites high mannose structures sites Glycoanalysis revealed predominantly high mannose structures present on most N-glycosylation sites, with limited evidence for complex glycosylation or processing to paucimannosidic forms. 29119999 10 50 gly peptide 1994:2000 arg1 the glycan topology and peptide sequence coverages the glycan topology and peptide sequence coverages In each case, IM-resolved CID and ETD events provided complete coverage of the glycan topology and peptide sequence coverages ranging from 48.4% (over 32 amino acid residues) to 85.7% (over eight amino acid residues). 29119999 10 105 gly sequence 2002:2009 arg1 the glycan topology and peptide sequence coverages the glycan topology and peptide sequence coverages In each case, IM-resolved CID and ETD events provided complete coverage of the glycan topology and peptide sequence coverages ranging from 48.4% (over 32 amino acid residues) to 85.7% (over eight amino acid residues). 28894650 3 31 gly glycosylated 625:636 arg1 peptides peptides peptides The specificity of lectins provides an added advantage of selecting peptides that are differently glycosylated and aberrantly expressed in cancer patients, many of which are not possibly detected using conventional methods because of their low abundance in bodily fluids. 28743910 10 29 gly glycoprotein 1415:1426 arg1 a P. acnes surface glycoprotein a P. acnes surface glycoprotein Fterm glycoprotein We describe here for the first time, the characterization of a P. acnes surface glycoprotein recognizing human fibrinogen. 25964011 8 44 gly glycosylation 1393:1405 arg2 active glycosylation sites active glycosylation sites sites Results obtained from deglycosylation experiments provided clear evidence of asparagine residues N44 and N39 representing active glycosylation sites in HEL. 25964011 8 56 gly residues 1352:1359 arg1 asparagine residues N44 and N39 asparagine residues N44 and N39 asparagine residues N44 and N39 Results obtained from deglycosylation experiments provided clear evidence of asparagine residues N44 and N39 representing active glycosylation sites in HEL. 25964011 8 56 gly residues 1352:1359 arg1 N39 asparagine residues N44 and N39 asparagine residues N44 and N39 Results obtained from deglycosylation experiments provided clear evidence of asparagine residues N44 and N39 representing active glycosylation sites in HEL. 25964011 8 56 gly residues 1352:1359 arg1 N39 asparagine residues N44 and N39 asparagine residues N44 and N39 Results obtained from deglycosylation experiments provided clear evidence of asparagine residues N44 and N39 representing active glycosylation sites in HEL. 24516574 7 52 gly glycoforms 1105:1114 arg1 bisecting GlcNAc bisecting GlcNAc This alteration in bisecting GlcNAc glycoforms as well as its corresponding association with ovarian cancer metastatic behavior was further validated at the glycotransferase level with multiple techniques including real-time PCR, western blotting, transwell assay, lectin blotting and immunohistochemistry analysis. 25451755 2 9 gly protein 317:323 arg1 carbohydrate protein carbohydrate Fterm protein The sulfated polysaccharide contained 92% of carbohydrate, 0% of protein, 7.8% of uronic acid, 22% of ash and 33% of moisture respectively. 28821844 1 15 gly N-glycosylated 192:205 arg1 these proteins these proteins Fterm proteins Nearly one third of the eukaryotic proteome traverses the secretory pathway and most of these proteins are N-glycosylated in the lumen of the endoplasmic reticulum. 24945804 12 99 gly pressure 2085:2092 arg1 the HA stem region the HA stem region the HA stem region Our findings indicate that StRAb binding to HA is precarious, raising the possibility that sufficient immune pressure on the HA stem region could select for viral escape mutants with increased steric hindrance from N-linked glycans. 28165356 8 74 gly glycoprotein 1579:1590 arg1 any physiologically or clinical relevant glycoprotein any physiologically or clinical relevant glycoprotein Fterm glycoprotein The methodology described, comparing a recombinant biopharmaceutical to its native equivalent, can be applied to any physiologically or clinical relevant glycoprotein. 27031228 9 29 gly glycopeptide 1151:1162 arg2 the synthetic glycopeptide hPDPN glycopeptide PUBTATOR hPDPN 10630 glycopeptide Furthermore, LpMab-12 reacted with the synthetic glycopeptide of hPDPN, corresponding to 38-54 amino acids (hpp3854: 38-EGGVAMPGAEDDVVTPG-54), which carries α2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52. 28826211 7 39 gly N-glycopeptides 1385:1399 arg2 N-glycopeptides N-glycopeptides N-glycopeptides Because of the excellent performance in glycopeptides enrichment, CSMs were applied to selectively enrich N-glycopeptides from tryptic digests of human serum and rat brain followed by nanoLC-MS/MS analysis. 28826211 7 61 gly glycopeptides 1319:1331 arg2 glycopeptides enrichment glycopeptides enrichment glycopeptides Because of the excellent performance in glycopeptides enrichment, CSMs were applied to selectively enrich N-glycopeptides from tryptic digests of human serum and rat brain followed by nanoLC-MS/MS analysis. 26721331 3 75 gly sialylated 539:548 arg1 sialylated terminal glycans sialylated terminal glycans METHODS MKN45 gastric carcinoma cells transfected with the sialyltransferase ST3GAL4 were established as a model overexpressing sialylated terminal glycans. 27026155 5 46 gly origin 997:1002 arg1 a ManC5-E origin a ManC5-E Fterm origin We report here the first heterologous production of a ManC5-E of brown algal origin that is successfully refolded in an active form. 25263911 6 29 gly regions 1126:1132 arg1 Polysaccharides regions Polysaccharides regions Polysaccharides from different parts or species of Ganoderma or polysaccharides from the same parts of Ganoderma but from different geographical regions or different strains could be differentiated clearly. 28053144 1 32 gly present 119:125 arg1 proteins AND glycans proteins glycans Fterm proteins Alterations of the structure and/or amount of glycans present on proteins are associated with many diseases. 28493026 7 50 gly glycoprotein 891:902 arg1 the glycans glycoprotein the glycans Fterm glycoprotein Agp is a O-linked glycoprotein with the glycans contributing to one-third of the molecular mass. 28473830 0 42 gly Hemagglutinin 44:56 arg1 N-Glycans Hemagglutinin N-Glycans Fterm Hemagglutinin The Length of N-Glycans of Recombinant H5N1 Hemagglutinin Influences the Oligomerization and Immunogenicity of Vaccine Antigen. 24753400 3 40 gly glycoproteins 634:646 arg1 almost all mammalian O-mannosyl glycoproteins almost all mammalian O-mannosyl glycoproteins Fterm glycoproteins With this in mind, we prepared a glycopeptide vaccine construct containing the N-acetyllactosamine-extended mannose motif Galβ1-4GlcNAcβ1-2ManαThr, found as a common core structure on almost all mammalian O-mannosyl glycoproteins identified. 24753400 3 46 gly glycopeptide 451:462 arg2 glycopeptide glycopeptide glycopeptide With this in mind, we prepared a glycopeptide vaccine construct containing the N-acetyllactosamine-extended mannose motif Galβ1-4GlcNAcβ1-2ManαThr, found as a common core structure on almost all mammalian O-mannosyl glycoproteins identified. 26572445 3 50 part_of calcium-LMP-based 514:530 arg1 homogeneous calcium-LMP-based composites LMP homogeneous calcium-LMP-based composites OGER Site LMP composites CF showed antioxidant activity and produced homogeneous calcium-LMP-based composites. 25832992 4 10 gly glycosylation 739:751 arg2 potential N-linked glycosylation sites potential N-linked glycosylation sites sites PNGase F itself contains potential N-linked glycosylation sites and we found that it was N-glycosylated when PNGase F secreted from silkworm cells. 29174358 5 95 part_of 1 929:929 arg1 C-6 position Araf, 1 C-6 position OGER Site Araf, 1 P10398 position The backbone of LBP1B-S-2 was consisted of 1, 3-linked β-d-Galp, 1, 6-linked β-d-Galp and branches contained 1, 4-linked β-d-GlcpA, T-linked β-d-Galp, 1, 6-linked β-d-Galp, T-linked α-l-Araf, T-linked β-l-Araf, 1, 5-linked α-l-Araf and T-linked β-l-Rhap directly or indirectly attached to C-3 position of 1, 6-linked β-d-Galp or C-6 position of 1, 3-linked β-d-Galp, according to the results of partial acid hydrolysis analysis, methylation analysis, IR and NMR spectra. 25092905 7 1 gly O-glycosites 1333:1344 arg2 1548 O-glycosites 1548 O-glycosites O-glycosites We identified 738 O-glycoproteins (1548 O-glycosites) in cell lysates and secretomes providing the first comprehensive insight into the O-glycosylation capacity of CHO (http://glycomics.ku.dk/o-glycoproteome_db/). 25092905 7 28 gly O-glycoproteins 1311:1325 arg1 1548 O-glycosites O-glycoproteins O-glycosites Fterm O-glycoproteins O-glycosites We identified 738 O-glycoproteins (1548 O-glycosites) in cell lysates and secretomes providing the first comprehensive insight into the O-glycosylation capacity of CHO (http://glycomics.ku.dk/o-glycoproteome_db/). 25817666 4 24 gly strain 759:764 arg1 polysaccharide chains strain polysaccharide chains Fterm strain This phase separation was correlated with lower dipolar interactions between polysaccharide chains and a decrease of ultimate strain and stress of films. 24416370 0 54 gly glycosylation 14:26 arg1 core 1 O-glycan-deficient mice core 1 O-glycan-deficient mice Altered mucus glycosylation in core 1 O-glycan-deficient mice affects microbiota composition and intestinal architecture. 28645725 9 2 gly sialylated 1364:1373 arg1 the total cell surface N-glycans the total cell surface N-glycans About one-third of the total cell surface N-glycans found on tet(-)HepAD38 were sialylated. 25271059 8 23 gly glycoprotein 1398:1409 arg1 nascent glycoprotein nascent glycoprotein Fterm glycoprotein We found that galactosyltransferase 4 is a major control point for glycan branching decisions taken in the Golgi of the cell, which might ultimately control the biological activity of nascent glycoprotein. 26367132 8 52 gly O-glycosylation 1557:1571 arg2 the first O-glycosylation site the first O-glycosylation site site Also, the first O-glycosylation site in a GH7 crystal structure is reported--on a loop where the glycan probably influences loop contacts across the active site and interactions with the cellulose surface. 26721331 8 44 gly sialylated 1186:1195 arg1 significantly increased sialylated N-glycans significantly increased sialylated N-glycans Sialoproteomic analysis further identified 47 proteins with significantly increased sialylated N-glycans. 26721331 8 49 gly proteins 1148:1155 arg1 significantly increased sialylated N-glycans proteins significantly increased sialylated N-glycans Fterm proteins Sialoproteomic analysis further identified 47 proteins with significantly increased sialylated N-glycans. 26477576 6 47 part_of GILT 1176:1179 arg1 a GILT signature sequence GILT a GILT signature sequence OGER Site GILT P13284 sequence The deduced protein possesses the typical structural features of known GILTs, including an active site motif, two potential N-linked glycosylation sites, a GILT signature sequence, and six conserved cysteines. 26477576 6 50 part_of possesses 1040:1048 arg1 The deduced protein AND an active site motif The deduced protein an active site motif Fterm Site protein motif The deduced protein possesses the typical structural features of known GILTs, including an active site motif, two potential N-linked glycosylation sites, a GILT signature sequence, and six conserved cysteines. 26477576 6 50 part_of possesses 1040:1048 arg1 The deduced protein AND two potential N-linked glycosylation sites The deduced protein two potential N-linked glycosylation sites Fterm Site protein sites The deduced protein possesses the typical structural features of known GILTs, including an active site motif, two potential N-linked glycosylation sites, a GILT signature sequence, and six conserved cysteines. 26477576 6 50 part_of possesses 1040:1048 arg1 The deduced protein AND six conserved cysteines The deduced protein six conserved cysteines Fterm AminoAcid protein cysteines The deduced protein possesses the typical structural features of known GILTs, including an active site motif, two potential N-linked glycosylation sites, a GILT signature sequence, and six conserved cysteines. 26477576 6 50 part_of possesses 1040:1048 arg1 The deduced protein AND a GILT signature sequence The deduced protein a GILT signature sequence Fterm Site protein sequence The deduced protein possesses the typical structural features of known GILTs, including an active site motif, two potential N-linked glycosylation sites, a GILT signature sequence, and six conserved cysteines. 29046357 4 56 gly glycopeptide 639:650 arg1 glycoprotein B glycoprotein B glycopeptide Cterm glycoprotein B glycopeptide We previously determined the crystal structure of PILRα complexed with the sTn-linked glycopeptide of glycoprotein B, revealing the simultaneous recognition of sTn and peptide by the receptor. 25832992 1 18 gly asparagine 262:271 arg1 the innermost GlcNAc and asparagine residues asparagine residues the innermost GlcNAc and asparagine residues asparagine residues The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 18 gly asparagine 262:271 arg1 high mannose, hybrid and complex oligosaccharides asparagine residues high mannose, hybrid and complex oligosaccharides asparagine residues The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 29 gly residues 273:280 arg1 glycoproteins glycoproteins residues Fterm glycoproteins The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 51 gly GlcNAc 251:256 arg1 glycoproteins glycoproteins GlcNAc Fterm glycoproteins The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 23 gly glycoproteins 340:352 arg1 the innermost GlcNAc and asparagine residues glycoproteins the innermost GlcNAc and asparagine residues Fterm glycoproteins The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 31 gly glycoproteins 340:352 arg1 glycoproteins glycoproteins the innermost GlcNAc and asparagine residues Fterm glycoproteins The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 31 gly glycoproteins 340:352 arg1 glycoproteins glycoproteins the innermost GlcNAc and asparagine residues Fterm glycoproteins The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 25832992 1 31 gly glycoproteins 340:352 arg1 glycoproteins glycoproteins high mannose, hybrid and complex oligosaccharides Fterm glycoproteins The peptide-N (4)-(N-acetyl-β-D-glucosaminyl) asparagine amidase F (PNGase F) catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from glycoproteins. 28990779 1 28 gly fucosylation 117:128 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins Core fucosylation of N-glycoproteins plays a crucial role in modulating the biological functions of glycoproteins. 28990779 1 52 gly glycoproteins 212:224 arg1 glycoproteins glycoproteins Fterm glycoproteins Core fucosylation of N-glycoproteins plays a crucial role in modulating the biological functions of glycoproteins. 28990779 1 71 gly N-glycoproteins 133:147 arg1 N-glycoproteins N-glycoproteins Core fucosylation Fterm N-glycoproteins Core fucosylation of N-glycoproteins plays a crucial role in modulating the biological functions of glycoproteins. 28990779 2 58 gly glycoproteins 293:305 arg1 structurally well-defined, core-fucosylated glycoproteins structurally well-defined, core-fucosylated glycoproteins Fterm glycoproteins Yet, the synthesis of structurally well-defined, core-fucosylated glycoproteins remains a challenging task due to the complexity in multistep chemical synthesis or the inability of the biosynthetic α1,6-fucosyltransferase (FUT8) to directly fucosylate full-size mature N-glycans in a chemoenzymatic approach. 28990779 2 72 gly fucosylate 468:477 arg1 full-size mature N-glycans full-size mature N-glycans Yet, the synthesis of structurally well-defined, core-fucosylated glycoproteins remains a challenging task due to the complexity in multistep chemical synthesis or the inability of the biosynthetic α1,6-fucosyltransferase (FUT8) to directly fucosylate full-size mature N-glycans in a chemoenzymatic approach. 24198434 6 26 gly N-glycosylation 1808:1822 arg2 the N-glycosylation sites the N-glycosylation sites sites The use of multiple lectins substantially increased N-glycoproteome coverage and although there were no discernible differences in the structures of N-glycans, or the charge, isoelectric point (pI) or hydrophobicity of the glycopeptides that differentially bound to each lectin, differences were observed in the amino acid frequency at the -1 and +1 subsites of the N-glycosylation sites. 24198434 6 95 gly glycopeptides 1665:1677 arg2 the glycopeptides the glycopeptides glycopeptides The use of multiple lectins substantially increased N-glycoproteome coverage and although there were no discernible differences in the structures of N-glycans, or the charge, isoelectric point (pI) or hydrophobicity of the glycopeptides that differentially bound to each lectin, differences were observed in the amino acid frequency at the -1 and +1 subsites of the N-glycosylation sites. 26424213 3 3 gly chain 527:531 arg1 their conserved threonine residue their conserved threonine residue their conserved threonine residue AminoAcid threonine residue Their glycosylated analogs-bearing lactose, maltose and cellobiose, as a glycan side chain on their conserved threonine residue, were synthesized where these disaccharides possess identical proximal sugar and vary in the nature of distal sugar and/or interglycosidic linkage. 28952521 3 85 gly N-glycosylation 414:428 arg2 up to five N-glycosylation sites up to five N-glycosylation sites sites IgA antibodies possess up to five N-glycosylation sites within their constant region of the heavy chain as compared to one site for IgG antibodies. 27440889 2 60 gly glycosylation 516:528 arg2 N-linked glycosylation sites N-linked glycosylation sites sites While N-linked glycosylation sites on the globular head are variable among different strains and different subtypes, N-linked glycosylation sites in the stem region are mostly well conserved among various influenza virus strains. 27440889 2 147 gly glycosylation 405:417 arg2 N-linked glycosylation sites N-linked glycosylation sites sites While N-linked glycosylation sites on the globular head are variable among different strains and different subtypes, N-linked glycosylation sites in the stem region are mostly well conserved among various influenza virus strains. 26751696 4 80 gly glycosylated 690:701 arg1 glycosylated α-dystroglycan glycosylated α-dystroglycan To gain insight into the role of glycosylated α-dystroglycan in these processes, we performed muscle fiber typing in young (2, 4 and 8 week old) and regenerated muscle. 26655794 2 18 gly attachment 342:351 arg2 soy peptides AND sugars soy peptides sugars peptides The covalent attachment of sugars to soy peptides was confirmed by amino acid analysis and examination of the Fourier-transform infrared spectra. 26689158 3 57 part_of heparin 559:565 arg1 the sequence heparin the sequence Fterm Site heparin sequence METHODS Disaccharide compositional analysis, utilizing heparinase-catalyzed depolymerization, is one of the most important ways to evaluate the sequence, structural composition and quality of heparin and LMWH. 26689158 3 57 part_of heparin 559:565 arg1 structural composition heparin structural composition Fterm Site heparin position METHODS Disaccharide compositional analysis, utilizing heparinase-catalyzed depolymerization, is one of the most important ways to evaluate the sequence, structural composition and quality of heparin and LMWH. 28371609 2 92 gly Asn297-linked 494:506 arg1 its Asn297-linked glycan Asn297 its Asn297-linked glycan Asn297 In this work, we first designed a pertuzumab plant biosimilar (PPB) and investigated the composition of its Asn297-linked glycan in comparison with trastuzumab plant biosimilar (TPB). 28964841 2 41 part_of lignin.The 429:438 arg1 lignin.The chemical composition lignin lignin.The chemical composition Fterm Site lignin position, Soda cooking resulted in reduction of recalcitrance of lignocellulose biomass leading to hydrolysis of hemicellulose into sugars, which was subsequently washed, leaving a residue of cellulose and lignin.The chemical composition, yield of lignin and cellulose were assessed using TAPPI method and ASTM standards, respectively. 27130732 4 14 gly glycoprotein 724:735 arg1 the glycoprotein the glycoprotein Fterm glycoprotein ISPD is a CDP-ribitol (ribose) pyrophosphorylase that generates the reduced sugar nucleotide for the insertion of ribitol in a phosphodiester linkage to the glycoprotein. 27031228 4 34 gly glycosylated 722:733 arg1 glycosylated membrane proteins glycosylated membrane proteins Fterm proteins We recently established CasMab technology to develop mAbs against glycosylated membrane proteins. 26857855 3 56 gly high-glycosylated 512:528 arg1 two isoforms two isoforms Fterm isoforms The AcFT enzyme showed two isoforms, low-glycosylated AcFT1 and high-glycosylated AcFT2 forms, with similar optimum activity at 60 °C. 26857855 3 72 gly low-glycosylated 485:500 arg1 two isoforms two isoforms Fterm isoforms The AcFT enzyme showed two isoforms, low-glycosylated AcFT1 and high-glycosylated AcFT2 forms, with similar optimum activity at 60 °C. 27440889 4 41 part_of HA2 951:953 arg1 the HA1 stem and HA2 stem regions HA1 the HA1 stem and HA2 stem regions OGER Site HA1 regions Since the HA stem region is constituted by an HA1 N-terminal part and a full HA2 part, we expressed a series of recombinant HA mutant proteins with deleted N-linked glycosylation sites in the HA1 stem and HA2 stem regions of H5N1 and pH1N1 viruses. 27440889 4 111 part_of deleted 894:900 arg1 deleted N-linked glycosylation sites proteins deleted N-linked glycosylation sites Fterm Site proteins sites Since the HA stem region is constituted by an HA1 N-terminal part and a full HA2 part, we expressed a series of recombinant HA mutant proteins with deleted N-linked glycosylation sites in the HA1 stem and HA2 stem regions of H5N1 and pH1N1 viruses. 27440889 4 158 part_of proteins 880:887 arg1 deleted N-linked glycosylation sites proteins deleted N-linked glycosylation sites Fterm Site proteins sites Since the HA stem region is constituted by an HA1 N-terminal part and a full HA2 part, we expressed a series of recombinant HA mutant proteins with deleted N-linked glycosylation sites in the HA1 stem and HA2 stem regions of H5N1 and pH1N1 viruses. 26477576 9 39 gly His6 1487:1490 arg1 a His6 tag His6 a His6 tag His6 Recombinant lbGILT was produced as an inclusion body with a His6 tag in ArcticExpress (DE3), and the protein was then washed, solubilized, and refolded. 27582506 4 5 part_of possesses 676:684 arg1 TRPA1 AND two putative N-linked glycosylation sites TRPA1 two putative N-linked glycosylation sites PUBTATOR Site TRPA1 8989 sites TRPA1 possesses two putative N-linked glycosylation sites at N747 and N753 that have not yet been studied in detail. 28213905 12 39 gly glycosylation 2108:2120 arg1 flagellin flagellin Fterm flagellin Taken together, this study reveals that the gigX cluster determines flagellin glycosylation, and implicates the regulatory role of post-translational modification with the glycosylation, acetylation and methylation of flagellin in the regulation of motility and virulence of Xoo. 28213905 8 46 gly glycosylated 1342:1353 arg1 the glycosylated flagellin the glycosylated flagellin Fterm flagellin In-frame deletion of each gigX gene affected flagellin glycosylation modification, meaning that the unglycosylated flagellin of the mutants was smaller than the glycosylated flagellin of the wild-type. 28213905 8 83 gly unglycosylated 1281:1294 arg1 the unglycosylated flagellin the unglycosylated flagellin Fterm flagellin In-frame deletion of each gigX gene affected flagellin glycosylation modification, meaning that the unglycosylated flagellin of the mutants was smaller than the glycosylated flagellin of the wild-type. 25253131 7 54 gly glycoprotein 1878:1889 arg1 the glycoprotein MUC1 the glycoprotein MUC1 Fterm glycoprotein We provide one example where a pull-down assay with all the GST-tagged canine galectins reveals that the C-terminal carbohydrate recognition domain of galectin-9 (Gal-9C) specifically recognizes the glycan-dependent apical targeting signal from the glycoprotein MUC1. 27796371 2 32 part_of hemagglutinin 360:372 arg1 the hemagglutinin globular head hemagglutinin the hemagglutinin globular head Fterm Site hemagglutinin head A decline in virulence has been accompanied by glycan accumulation on the hemagglutinin globular head, and hemagglutinin receptor binding has changed from recognition of a broad spectrum of glycan receptors to a narrower spectrum. 24333678 4 47 part_of [CEL/CS+B15C5 572:584 arg1 The [CEL/CS+B15C5] composites CEL The [CEL/CS+B15C5] composites PUBTATOR Site CEL P19835 composites The [CEL/CS+B15C5] composites obtained retain properties of their components, namely superior mechanical strength (from CEL), excellent adsorption capability for heavy metal ions and organic pollutants (from B15C5 and CS). 27616693 4 19 part_of PDP 578:580 arg1 the composition PDP the composition OGER Site PDP position Following analysis by IR, HPLC-UV, MS and 1H NMR, the composition of PDP was found to be l-rhamnose, galactose, arabinose, glucose and d-galacturonic acid. 26367394 8 87 gly glycosylation 1063:1075 arg2 up to 5 glycosylation sites up to 5 glycosylation sites sites Unlike class I pilins that carry a single glycan, we found that class II pilins display up to 5 glycosylation sites per monomer on the pilus surface. 26093664 3 45 gly sialylated 794:803 arg1 sialylated ApoC-III isoforms sialylated ApoC-III isoforms The study's aim was to establish this method in our laboratory, what included performing the analysis in a group of 170 healthy individuals to set the reference range of detected relative amounts of sialylated ApoC-III isoforms and to evaluate the gender- and age-dependent differences. 28683274 10 6 part_of β-d-Gal 1404:1410 arg1 alternating 4-linked α-d-Gal and 3-linked β-d-Gal residues Gal alternating 4-linked α-d-Gal and 3-linked β-d-Gal residues OGER Site Gal P22466 residues The third polysaccharide is a xylo(fuco)galactan having a linear core of alternating 4-linked α-d-Gal and 3-linked β-d-Gal residues. 28683274 10 28 part_of α-d-Gal 1383:1389 arg1 alternating 4-linked α-d-Gal and 3-linked β-d-Gal residues Gal alternating 4-linked α-d-Gal and 3-linked β-d-Gal residues OGER Site Gal P22466 residues The third polysaccharide is a xylo(fuco)galactan having a linear core of alternating 4-linked α-d-Gal and 3-linked β-d-Gal residues. 24948903 9 2 gly 91 N-glycosylation 1635:1652 arg2 91 N-glycosylation sites 91 N-glycosylation sites sites We identify 91 N-glycosylation sites derived from 51 secreted proteins, as well as 155 and 29 released N- and O-glycans respectively. 25766612 6 59 gly Asn 947:949 arg1 N-glycan Asn(204) N-glycan Asn(204) Our study of the association of N-glycan of G protein at Asn(204) with the transport of RABV G protein to the cell surface membrane contributes to the understanding of the evolution of fixed virus from street virus, which in turn would help for determine the mechanism underlying RABV budding and enhanced host immune responses. 24334764 2 85 gly glycosylation 336:348 arg1 newly synthesized proteins newly synthesized proteins Fterm proteins As the Golgi complex is the place where glycosylation of newly synthesized proteins occurs, we hypothesized that VPS13B deficiency, responsible of Golgi apparatus disturbance, could lead to glycosylation defects and/or mysfunction of this organelle, and thus be a cause of the main clinical manifestations of CS. 26160171 9 51 part_of mucin-like 1523:1532 arg1 the mucin-like region mucin the mucin-like region PUBTATOR Site mucin 100508689 region Taken together, our data suggest that the mucin-like region of HSV-1 gC is involved in the modulation of the GAG-binding activity, a feature of importance both for unrestricted virus entry into the cells and release of newly produced viral particles from infected cells. 25117255 4 3 gly have 841:844 arg1 a putative lectin domain AND sugar-binding ability a putative lectin domain sugar-binding ability domain This strategy is highly sensitive, and useful to determine whether or not a putative lectin domain may have sugar-binding ability. 28444931 9 33 part_of haptoglobin 1137:1147 arg1 a glycopeptide haptoglobin a glycopeptide PUBTATOR Site haptoglobin 3240 glycopeptide We then demonstrated on the case of a glycopeptide of haptoglobin that the predicted retention time window boosts confidence of identification and minimizes false-positive identification. 25832992 2 40 gly glycoproteins 478:490 arg1 the glycoproteins the glycoproteins Fterm glycoproteins The PNGase F has broad substrate specificity and thus is extensively used for the structural and functional studies of the glycoproteins. 29046357 3 49 gly sialylated 473:482 arg1 sTn sTn the sialylated O-linked sugar T antigen OGER sTn Q05639 PILRα is the only Siglec member to recognize not only the sialylated O-linked sugar T antigen (sTn) but also its attached peptide region. 25779987 7 91 gly glycoprotein 1258:1269 arg1 α1-acid glycoprotein α1-acid glycoprotein Fterm glycoprotein RESULTS Major acute-phase protein contributors to the serum GlycA signal are α1-acid glycoprotein, haptoglobin, α1-antitrypsin, α1-antichymotrypsin, and transferrin. 27213484 7 20 part_of BC-pectin 1432:1440 arg1 BC-pectin composites pectin BC-pectin composites Fterm Site pectin composites This finding was consistent with cellulose, not pectin, being the major load bearing component in BC-pectin composites, as is also the case in plant cell walls. 25261472 7 47 gly glycoproteins 1024:1036 arg1 EMV glycoproteins EMV glycoproteins Fterm glycoproteins We identify EMV glycoproteins and demonstrate alteration of their recruitment as a function of their glycosylation status upon pharmacological manipulation. 24526361 6 11 gly glycoproteins 1190:1202 arg1 glycoproteins glycoproteins Fterm glycoproteins Here we screen a library of α-mannosidases for their ability to decrease the extent of O-mannosylation on glycoproteins secreted from this expression system. 28342150 9 10 gly glycoproteins 1590:1602 arg1 high-quality recombinant glycoproteins high-quality recombinant glycoproteins Fterm glycoproteins Taken together, rhβ4GalT1 from silkworms can become a valuable tool for producing high-quality recombinant glycoproteins with mammalian-like N-glycans. 27413183 3 8 gly glycoproteins 663:675 arg1 glycoproteins glycoproteins Fterm glycoproteins Although the lectin-oligosaccharide association is well understood, the polypeptide-based interaction is more controversial because the binding site on calreticulin has not been identified, and its significance in the biogenesis of glycoproteins in cells remains unknown. 27112851 1 56 part_of NCC 233:235 arg1 nanocrystalline cellulose (NCC) filled polylactide (PLA) composites NCC nanocrystalline cellulose (NCC) filled polylactide (PLA) composites OGER Site NCC P55017 composites A 'continuous route' was developed in this work for the preparation of nanocrystalline cellulose (NCC) filled polylactide (PLA) composites. 27566209 4 95 gly homogeneity 685:695 arg1 the two polysaccharides the two polysaccharides The homogeneity, molecular weight, monosaccharide composition and linkage of the two polysaccharides were determined in addition to other chemical characterizations. 24975464 2 50 part_of IgG 454:456 arg1 the heavy chain constant domains IgG the heavy chain constant domains Cterm Site IgG domains In this study, we developed a pE16 variant consisting of a single-chain variable fragment (scFv) fused to the heavy chain constant domains (CH) of human IgG (pE16scFv-CH). 24085812 5 82 gly glycoproteins 635:647 arg1 Fucosylated glycoproteins Fucosylated glycoproteins Fterm glycoproteins Fucosylated glycoproteins were enriched by the Aleuria aurantia lectin column after serum albumin and IgG depletion. 25782436 9 66 gly protein 1255:1261 arg1 the sialic acid content protein the sialic acid content Fterm protein CONCLUSION SEC-HPLC is a promising PAT tool to monitor the sialic acid content of Fc-fusion protein during biomanufacturing or medium optimization processes. 27582506 5 17 gly modified 833:840 arg1 these sites AND an N-glycan and that the glycan at position N747 modulates agonist-sensitivity of TRPA1 in vitro Additionally, we found that N-glycosylation also modulates cooperative effects of temperature and the agonist cinnamaldehyde on TRPA1 channel activation these sites an N-glycan and that the glycan at position N747 modulates agonist-sensitivity of TRPA1 in vitro Additionally, we found that N-glycosylation also modulates cooperative effects of temperature and the agonist cinnamaldehyde on TRPA1 channel activation sites Here, we show that both of these sites can be modified with an N-glycan and that the glycan at position N747 modulates agonist-sensitivity of TRPA1 in vitro Additionally, we found that N-glycosylation also modulates cooperative effects of temperature and the agonist cinnamaldehyde on TRPA1 channel activation. 25782436 0 37 gly protein 43:49 arg1 Monitoring sialylation levels protein Monitoring sialylation levels Fterm protein Monitoring sialylation levels of Fc-fusion protein using size-exclusion chromatography as a process analytical technology tool. 28955895 5 15 gly glycosylation 787:799 arg2 glycosylation sites glycosylation sites sites We showed that part of glycosylation sites was important for folding of the proteins involved in the formation of viral particles. 26367132 12 45 part_of cellobiohydrolase 2182:2198 arg1 The Geotrichum candidum GH family 7 cellobiohydrolase nucleotide sequence cellobiohydrolase The Geotrichum candidum GH family 7 cellobiohydrolase nucleotide sequence Fterm Site cellobiohydrolase sequence The Geotrichum candidum GH family 7 cellobiohydrolase nucleotide sequence is available in GenBank under accession number KJ958925. 25320359 10 44 gly glycoproteins 1543:1555 arg1 new PDAC-derived O-GalNAc glycoproteins new PDAC-derived O-GalNAc glycoproteins We identified new PDAC-derived O-GalNAc glycoproteins by mass spectrometry using a blood group A-specific antibody. 27833622 16 2 part_of Araf 2219:2222 arg1 fewer Araf residues Araf fewer Araf residues OGER AminoAcid Araf P10398 residues in A decrease in cell wall-associated arabinose was expected, which was likely caused by fewer Araf residues in the arabinoxylan. 27492264 3 19 gly asparagine-linked 497:513 arg1 an asparagine-linked (N-)glycan asparagine an asparagine-linked (N-)glycan asparagine This interaction requires the presence of an asparagine-linked (N-)glycan on the Fc, and variations in the N-glycan composition can affect the affinity of CD16A binding (an FcγR). 27716795 1 23 gly glycosylation 123:135 arg1 the envelope (Env) surface subunit the envelope (Env) surface subunit Fterm subunit 155971 Heavy glycosylation of the envelope (Env) surface subunit, gp120, is a key adaptation of HIV-1; however, the precise effects of glycosylation on the folding, conformation and dynamics of this protein are poorly understood. 27716795 1 27 gly glycosylation 245:257 arg1 this protein this protein Fterm protein 155971 Heavy glycosylation of the envelope (Env) surface subunit, gp120, is a key adaptation of HIV-1; however, the precise effects of glycosylation on the folding, conformation and dynamics of this protein are poorly understood. 27031228 3 40 gly glycosylated 636:647 arg1 glycosylated Thr52 glycosylated Thr52 Thr52 Although various anti-hPDPN monoclonal antibodies (mAbs) have been generated, no specific mAb has been reported to target the epitope containing glycosylated Thr52. 28576849 12 63 gly glycoprotein 2016:2027 arg1 the identified specific glycoprotein ligands the identified specific glycoprotein ligands Fterm glycoprotein In conclusion, the identified specific glycoprotein ligands shed light into the highly specific binding of galectins to dedifferentiated RPE cells and the resulting prevention of PVR-associated cellular events. 24904645 5 13 gly glycosylation 1058:1070 arg1 protein structure protein structure Fterm protein Therefore glycans enable adaptive response to environmental changes and, unlike other epiproteomic modifications, which act as off/on switches, glycosylation significantly contributes to protein structure and enables novel functions. 28959962 2 44 gly glycosylated 250:261 arg1 human proteins human proteins Fterm proteins Over 50% of human proteins are glycosylated, which alters their activities in diverse fundamental biological processes. 26618514 5 60 gly non-sialylated 828:841 arg1 the non-sialylated fraction the non-sialylated fraction Analysing the sialylation status of affinity-purified anti-histone IgG in patients with systemic lupus erythematosus (SLE), we demonstrated that the anti-histone IgG was contained preferentially in the non-sialylated fraction. 28636500 2 10 gly glycosylated 373:384 arg1 aberrantly glycosylated O-glycans aberrantly glycosylated O-glycans In the serum of patients with IgAN, the hinge region of IgA1 immunoglobulin contains aberrantly glycosylated O-glycans deficient in galactose, which is normally added to the core 1 O-glycan structure by core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1), the key enzyme in the process of glycosylation. 28636500 2 36 gly contains 353:360 arg1 the hinge region AND aberrantly glycosylated O-glycans the hinge region the hinge region aberrantly glycosylated O-glycans region In the serum of patients with IgAN, the hinge region of IgA1 immunoglobulin contains aberrantly glycosylated O-glycans deficient in galactose, which is normally added to the core 1 O-glycan structure by core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1), the key enzyme in the process of glycosylation. 28821844 2 30 gly glycoproteins 361:373 arg1 many glycoproteins many glycoproteins Fterm glycoproteins N-glycans fulfill multiple structural and biological functions, and are crucial for productive folding of many glycoproteins. 27756641 3 18 part_of α-amylase 779:787 arg1 the tryptophan residues α-amylase the tryptophan residues Fterm AminoAcid α-amylase tryptophan residues Synchronous fluorescence spectroscopy revealed that the binding interaction between the tryptophan residues of α-amylase and TPSA was predominant. 25378534 5 74 part_of IgG 1089:1091 arg1 the IgG Fab region IgG the IgG Fab region Cterm Site IgG region Interestingly, we found that several regions in the IgG Fab region also showed reduced deuterium uptake. 25378534 5 84 part_of Fab 1093:1095 arg1 the IgG Fab region Fab the IgG Fab region PUBTATOR Site Fab 2187 region Interestingly, we found that several regions in the IgG Fab region also showed reduced deuterium uptake. 25378534 5 88 part_of regions 1074:1080 arg1 the IgG Fab region regions the IgG Fab region region Interestingly, we found that several regions in the IgG Fab region also showed reduced deuterium uptake. 27294781 1 50 gly glycosylated 376:387 arg1 its neuronal receptor its neuronal receptor Fterm receptor Here we report a 2.0-Å-resolution crystal structure of the BoNT/A1 receptor-binding domain in complex with its neuronal receptor, glycosylated human SV2C. 28554385 1 31 gly N-glycosylation 253:267 arg2 three N-glycosylation sites three N-glycosylation sites sites Terminal sialic acids on N-glycan of recombinant human erythropoietin are very important for in vivo half-life, as this glycoprotein has three N-glycosylation sites. 28554385 1 78 gly glycoprotein 230:241 arg1 this glycoprotein this glycoprotein Fterm glycoprotein Terminal sialic acids on N-glycan of recombinant human erythropoietin are very important for in vivo half-life, as this glycoprotein has three N-glycosylation sites. 29130773 8 28 gly glycoproteins 849:861 arg1 The glycoproteins The glycoproteins Fterm glycoproteins RESULTS The glycoproteins recognized by three lectins [Aleuria aurantia lectin (AAL); Phytolacca americana (PWM); Phaseolus vulgaris agglutinin (E + L), (PHA-E + L)] were mainly increasing in the saliva of OLP. 26093517 2 29 part_of residues 406:413 arg1 proteins proteins residues Fterm AminoAcid proteins asparagine residues The OST enzymes catalyze the transfer of an oligosaccharide chain from lipid-linked oligosaccharides (LLO) to asparagine residues in proteins. 26353993 1 8 gly glycosylation 191:203 arg2 glycosylation site glycosylation site site It has been previously reported that the glycosylation site and protein-sequence information could be obtained for ribonuclease B by top-down electron-capture dissociation (ECD) and collision-induced dissociation (CID) mass spectrometry (MS). 28990779 6 92 part_of N-glycoproteins 1564:1578 arg1 N-glycopeptides N-glycoproteins N-glycopeptides Fterm Site N-glycoproteins N-glycopeptides This discovery opens a new avenue to a wide variety of homogeneous, core-fucosylated N-glycopeptides and N-glycoproteins that are hitherto difficult to obtain for structural and functional studies. 27213484 3 1 part_of BC 696:697 arg1 Komagataeibacter xylinus bacterial cellulose (BC)-pectin composites BC Komagataeibacter xylinus bacterial cellulose (BC)-pectin composites Cterm Site BC composites To overcome some of these difficulties, we report on a novel method for investigating Al interactions with Komagataeibacter xylinus bacterial cellulose (BC)-pectin composites as cell wall analogues. 27213484 3 7 part_of -pectin 699:705 arg1 Komagataeibacter xylinus bacterial cellulose (BC)-pectin composites pectin Komagataeibacter xylinus bacterial cellulose (BC)-pectin composites Fterm Site pectin composites To overcome some of these difficulties, we report on a novel method for investigating Al interactions with Komagataeibacter xylinus bacterial cellulose (BC)-pectin composites as cell wall analogues. 28645725 11 64 gly fucosylated 1556:1566 arg1 the total fucosylated type the total fucosylated type Neither treatment changed the cell surface N-glycans expression of the total complex type or the total fucosylated type, which were about 50% or 60%, respectively. 28444931 9 79 gly glycopeptide 1121:1132 arg1 haptoglobin haptoglobin glycopeptide PUBTATOR haptoglobin 3240 glycopeptide We then demonstrated on the case of a glycopeptide of haptoglobin that the predicted retention time window boosts confidence of identification and minimizes false-positive identification. 28213905 11 107 gly glycosylated 1792:1803 arg1 the glycosylated flagellin the glycosylated flagellin Fterm flagellin Moreover, the glycosylated flagellin of the wild-type induced higher H2 O2 levels in rice leaves than did the unglycosylated flagellins of ΔgigX1 or ΔgigX10. 25256490 0 41 gly pumpkin 90:96 arg1 polysaccharides pumpkin polysaccharides Fterm pumpkin Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata). 29046357 7 39 gly glycopeptides 1097:1109 arg2 glycopeptides glycopeptides glycopeptides We show that glycopeptides with different sugar units linking SA and peptides (i.e. "GlcNAc-type" and "deoxy-GlcNAc-type" glycopeptides) have lower affinity and more enthalpy-driven binding than the wild type (i.e. GalNAc-type glycopeptide). 29046357 7 55 gly " 1071:1071 arg1 glycopeptides glycopeptides glycopeptides We show that glycopeptides with different sugar units linking SA and peptides (i.e. "GlcNAc-type" and "deoxy-GlcNAc-type" glycopeptides) have lower affinity and more enthalpy-driven binding than the wild type (i.e. GalNAc-type glycopeptide). 29046357 7 32 gly glycopeptides 988:1000 arg1 different sugar units glycopeptides different sugar units glycopeptides We show that glycopeptides with different sugar units linking SA and peptides (i.e. "GlcNAc-type" and "deoxy-GlcNAc-type" glycopeptides) have lower affinity and more enthalpy-driven binding than the wild type (i.e. GalNAc-type glycopeptide). 24948903 8 51 gly N-glycosylation 1411:1425 arg2 the sites the sites sites Moreover, we apply glycomic techniques to examine (1) the sites of N-glycosylation on secreted proteins, (2) the structures of complex N- and O-glycans, and (3) the relative abundance of complex N- and O-glycans structures in insulin responsive and insulin resistant conditions. 24608122 11 15 gly glycoprotein 1426:1437 arg1 the human glycoprotein hLF the human glycoprotein hLF Fterm glycoprotein The activity on the human glycoprotein hLF, and the functional consequences with reduced inhibition of biofilm formation highlights both innate immunity functions of hLF and a bacterial mechanism to evade this innate immunity function. 27943633 7 24 gly disialylated 1441:1452 arg1 more than 62% biantennary disialylated glycans more than 62% biantennary disialylated glycans Finally, glycoprofiling of IgG with four amino acid substitutions expressed from an α-2,3 sialyltransferase knockout-α-2,6 sialyltransferase stable clone resulted in more than 77% sialylated glycans and more than 62% biantennary disialylated glycans as indicated by both MALDI-TOF and LC-ESI-MS. 27943633 7 38 gly sialylated 1392:1401 arg1 more than 77% sialylated glycans more than 77% sialylated glycans Finally, glycoprofiling of IgG with four amino acid substitutions expressed from an α-2,3 sialyltransferase knockout-α-2,6 sialyltransferase stable clone resulted in more than 77% sialylated glycans and more than 62% biantennary disialylated glycans as indicated by both MALDI-TOF and LC-ESI-MS. 28342150 6 14 gly N-glycosylated 1122:1135 arg1 rhβ4GalT1 rhβ4GalT1 We demonstrated that rhβ4GalT1 is N-glycosylated and without mucin-type glycosylation. 25523794 4 12 gly N-glycosylation 795:809 arg2 all N-glycosylation sites all N-glycosylation sites sites Mass spectrometry analyses showed that all N-glycosylation sites of the extracellular domain of the recombinant haemagglutinins carry plant-specific complex-type N-glycans having core α(1,3)-fucose, core β(1,2)-xylose epitopes and Lewis(a) extensions. 25523794 4 60 gly carry 880:884 arg1 the extracellular domain AND plant-specific complex-type N-glycans the extracellular domain plant-specific complex-type N-glycans domain Mass spectrometry analyses showed that all N-glycosylation sites of the extracellular domain of the recombinant haemagglutinins carry plant-specific complex-type N-glycans having core α(1,3)-fucose, core β(1,2)-xylose epitopes and Lewis(a) extensions. 25523794 4 60 gly carry 880:884 arg1 all N-glycosylation sites AND plant-specific complex-type N-glycans all N-glycosylation sites plant-specific complex-type N-glycans sites Mass spectrometry analyses showed that all N-glycosylation sites of the extracellular domain of the recombinant haemagglutinins carry plant-specific complex-type N-glycans having core α(1,3)-fucose, core β(1,2)-xylose epitopes and Lewis(a) extensions. 25523794 4 12 gly N-glycosylation 795:809 arg2 the extracellular domain domain domain Mass spectrometry analyses showed that all N-glycosylation sites of the extracellular domain of the recombinant haemagglutinins carry plant-specific complex-type N-glycans having core α(1,3)-fucose, core β(1,2)-xylose epitopes and Lewis(a) extensions. 25434844 2 4 gly pectin 456:461 arg1 monosaccharide composition pectin monosaccharide composition Fterm pectin In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and monosaccharide composition of pectin and hemicelluloses from five sweet cherry cultivars ('Chelan', 'Sumele', 'Brooks', 'Sunburst', and 'Regina') with contrasting firmness and cracking susceptibility at two developmental stages (immature and ripe). 27743368 1 46 gly Glycosylation 56:68 arg1 membrane proteins membrane proteins Fterm proteins Glycosylation of membrane proteins plays a crucial role in various physiological events, including intercellular recognition and intermolecular interactions on the cell surface (Gornik et al., Biochim Biophys Acta 1820:1318-1326, 2012). 29292146 6 72 gly residues 1076:1083 arg1 oligosaccharides residues oligosaccharides residues One domain contains a RG I-type backbone with branching at O-3 of GalpA residues to terminal β-D-GlcpA residues, another similarly contains a RG I-type backbone but is branched at O-4 of the Rhap residues to terminal GalpA residues or oligosaccharides containing α-linked 4-Galp and terminal GalpA residues. 25378534 4 10 part_of Fc 863:864 arg1 the antibody Fc region Fc the antibody Fc region Cterm Site Fc region Several regions in the antibody Fc region and the FcRn were protected from exchange upon complex formation, in good agreement with previous crystallographic studies of FcRn in complex with the Fc fragment. 25378534 4 27 part_of regions 839:845 arg1 the antibody Fc region regions the antibody Fc region region Several regions in the antibody Fc region and the FcRn were protected from exchange upon complex formation, in good agreement with previous crystallographic studies of FcRn in complex with the Fc fragment. 25378534 4 27 part_of regions 839:845 arg1 the FcRn FcRn regions PUBTATOR Site FcRn 2217 regions Several regions in the antibody Fc region and the FcRn were protected from exchange upon complex formation, in good agreement with previous crystallographic studies of FcRn in complex with the Fc fragment. 25378534 4 52 part_of Fc 1024:1025 arg1 the Fc fragment Fc the Fc fragment Cterm Site Fc fragment Several regions in the antibody Fc region and the FcRn were protected from exchange upon complex formation, in good agreement with previous crystallographic studies of FcRn in complex with the Fc fragment. 24980207 0 41 gly glycosylation 45:57 arg1 a plant latex peroxidase a plant latex peroxidase Fterm peroxidase Post-translational modification and extended glycosylation pattern of a plant latex peroxidase of native source characterized by X-ray crystallography. 25964011 3 36 gly glycosylation 358:370 arg2 active glycosylation sites active glycosylation sites sites 1995, 73, 307-309) proposed the existence of a low abundant N-glycosylated form of HEL; however, the identity of active glycosylation sites in HEL remained a matter of speculation. 25862865 0 51 gly glycosylation 9:21 arg1 αvβ3 integrin αvβ3 integrin Fterm integrin Aberrant glycosylation of αvβ3 integrin is associated with melanoma progression. 25855670 9 78 gly glycoproteins 1396:1408 arg1 oocyte glycoproteins oocyte glycoproteins Fterm glycoproteins These data reveal that oocyte glycoproteins carrying core 1-derived O-glycans have a regulatory role in COC development. 25855670 9 13 gly carrying 1410:1417 arg1 oocyte glycoproteins AND core 1-derived O-glycans oocyte glycoproteins core 1-derived O-glycans Fterm glycoproteins These data reveal that oocyte glycoproteins carrying core 1-derived O-glycans have a regulatory role in COC development. 27659420 2 45 gly asparagine-linked 234:250 arg1 asparagine-linked oligosaccharides asparagine asparagine-linked oligosaccharides asparagine N-Acetylglucosaminyltransferase V (GnT-V), catalyzing β1-6 branching in asparagine-linked oligosaccharides, is one of the most important glycosyltransferases involved in cancer and the immune system. 26512888 3 20 gly glycoproteins 419:431 arg1 cell surface glycoproteins cell surface glycoproteins Fterm glycoproteins Here we report that CTB binds cell surface glycoproteins. 28955895 7 70 part_of RNA 1398:1400 arg1 RNA fragments RNA RNA fragments Cterm Site RNA fragments Our data showed that, in contrast to HCV 1a and 2a, the folding of HCV 1b envelope proteins E2 (sites N1, N2, N10) and E1 (sites N1, N5) was disrupted, however that did not prevent the formation of virus-like particles (VLP) with misfolded glycoproteins having densities typical for HCV particles containing RNA fragments. 24746228 2 74 gly glycosylated 515:526 arg1 adsorbed proteins adsorbed proteins Fterm proteins Additionally, the innate inflammatory response to implanted biomaterials has been hypothesized to be mediated by inflammatory cells interacting with adsorbed proteins, many of which are glycosylated. 27413183 5 21 gly non-glycosylated 1019:1034 arg1 non-glycosylated firefly luciferase non-glycosylated firefly luciferase Fterm luciferase Mutations in only one patch, P19K/I21E and Y22K/F84E, impaired the ability of calreticulin to suppress the thermally induced aggregation of non-glycosylated firefly luciferase. 25378534 6 25 part_of Fab 1227:1229 arg1 the Fab region Fab the Fab region PUBTATOR Site Fab 2187 region Our findings indicate the presence of hitherto unknown FcRn interaction sites in the Fab region or a possible conformational link between the IgG Fc and Fab regions upon FcRn binding. 25378534 6 61 part_of Fc 1288:1289 arg1 the IgG Fc and Fab regions Fc the IgG Fc and Fab regions Cterm Site Fc regions Our findings indicate the presence of hitherto unknown FcRn interaction sites in the Fab region or a possible conformational link between the IgG Fc and Fab regions upon FcRn binding. 25378534 6 76 part_of Fab 1295:1297 arg1 the IgG Fc and Fab regions Fab the IgG Fc and Fab regions PUBTATOR Site Fab 2187 regions Our findings indicate the presence of hitherto unknown FcRn interaction sites in the Fab region or a possible conformational link between the IgG Fc and Fab regions upon FcRn binding. 25378534 6 81 part_of FcRn 1197:1200 arg1 hitherto unknown FcRn interaction sites FcRn hitherto unknown FcRn interaction sites PUBTATOR Site FcRn 2217 sites Our findings indicate the presence of hitherto unknown FcRn interaction sites in the Fab region or a possible conformational link between the IgG Fc and Fab regions upon FcRn binding. 26093517 10 56 gly linked 1642:1647 arg1 the Asn residue AND the glucose residue the Asn residue the glucose residue Asn residue The MS analysis of the minor HPLC peak of the in vitro oligosaccharyl transfer products also revealed an optional sulfate modification on the glucose residue directly linked to the Asn residue. 26655437 10 60 gly glycoprotein 1610:1621 arg1 glycoprotein glycoprotein Fterm glycoprotein Thus, besides studying the glycosylation pattern of the entire placental membrane due to preeclampsia, it is inevitable to study directly glycoprotein of interest, as no general assumptions or extrapolations can be made. 24796651 6 93 gly glycopeptide 1319:1330 arg2 intact glycopeptide positive spectrum matches intact glycopeptide positive spectrum matches glycopeptide We demonstrated the feasibility and advantage of identifying the peptide core ion directly from edited HCD-MS(2) data as an easy way to reduce false positives without compromising much sensitivity in intact glycopeptide positive spectrum matches. 24573103 1 56 gly glycosylation 121:133 arg1 cell surface glycoproteins cell surface glycoproteins Fterm glycoproteins Aberrant glycosylation of cell surface glycoproteins is commonly associated with the invasion and metastasis of colorectal carcinomas, which can be attributed to the upregulated expression of glycosyltransferases. 24573103 1 62 gly glycoproteins 151:163 arg1 cell surface glycoproteins cell surface glycoproteins Fterm glycoproteins Aberrant glycosylation of cell surface glycoproteins is commonly associated with the invasion and metastasis of colorectal carcinomas, which can be attributed to the upregulated expression of glycosyltransferases. 28407380 5 66 gly glycoproteins 995:1007 arg1 the main glycoproteins the main glycoproteins Fterm glycoproteins Mass spectrometry also suggested that the Lotus japonicus convicilin 2 was one of the main glycoproteins undergoing differential expression/N-glycosylation in the mutants. 27440889 10 157 gly glycosylation 1921:1933 arg2 N-linked glycosylation sites N-linked glycosylation sites sites N-linked glycosylation sites in the stem regions of influenza virus hemagglutinin (HA) proteins are mostly well conserved among various influenza virus strains. 27716795 5 91 gly isolates 1018:1025 arg1 N156 N156 and N160 N156 and N160 Simulations were performed with and without N-linked glycosylation at two sites that are highly conserved across HIV-1 isolates (N156 and N160); both are contacts for recognition by V1V2-targeted broadly neutralizing antibodies against HIV-1. 28444931 3 69 gly glycopeptides 388:400 arg2 glycopeptides glycopeptides glycopeptides In this work, we show the effect of various monosaccharide units on the retention time of glycopeptides. 24531467 3 7 gly glycosylation 643:655 arg2 two glycosylation sites two glycosylation sites sites Here, the structure of the endogenous isoform II of Hev b 2 that exhibits three post-translational modifications, including an N-terminal pyroglutamate and two glycosylation sites at Asn27 and at Asn314, is reported from two crystal polymorphs. 28471203 9 104 gly released 1152:1159 arg1 proteins AND Glycans proteins Glycans Fterm proteins Glycans were released from proteins by the enzyme PNGase F and proteins were digested to peptides by trypsin. 28444931 6 19 gly Fucosylation 746:757 arg1 larger glycans larger glycans Fucosylation of larger glycans provides less significant retention time shift than for smaller ones. 28019699 4 19 part_of EGFR 882:885 arg1 the EGFR extracellular domain EGFR the EGFR extracellular domain PUBTATOR Site EGFR 1956 domain Molecular dynamics simulations were performed to characterize the conformational preferences of the monomeric and dimeric forms of the EGFR extracellular domain upon binding to EGF in the presence and absence of N-glycan moieties. 25885924 3 29 gly glycoproteins 462:474 arg1 Gal-containing glycoproteins Gal-containing glycoproteins ldlD-14 cells, a CHO cell mutant deficient in UDP-Gal 4-epimerase, are useful for structural and functional studies of Gal-containing glycoproteins because their abnormal glycosylation can be converted to normal status by exogenous addition of galactose (Gal). 27584569 1 2 gly fucosylated 164:174 arg1 biantennary core fucosylated glycans biantennary core fucosylated glycans VWF is extensively glycosylated with biantennary core fucosylated glycans. 24721674 11 19 gly composition 2233:2243 arg1 sequence sequence sequence Totally 811 N-glycosylation sites from 567 proteins were identified and 177 intact glycopeptides with both glycan composition and peptides sequence were characterized, which provided a solution for site-specific N-glycosylation characterization of membrane. 24721674 11 100 gly N-glycosylation 2131:2145 arg2 811 N-glycosylation sites 811 N-glycosylation sites sites Totally 811 N-glycosylation sites from 567 proteins were identified and 177 intact glycopeptides with both glycan composition and peptides sequence were characterized, which provided a solution for site-specific N-glycosylation characterization of membrane. 24721674 11 112 gly glycopeptides 2202:2214 arg2 177 intact glycopeptides glycopeptides glycan composition glycopeptides Totally 811 N-glycosylation sites from 567 proteins were identified and 177 intact glycopeptides with both glycan composition and peptides sequence were characterized, which provided a solution for site-specific N-glycosylation characterization of membrane. 29150937 0 37 gly glycoprotein 102:113 arg1 an extensively glycosylated, trimeric HIV-1 envelope glycoprotein an extensively glycosylated, trimeric HIV-1 envelope glycoprotein Fterm glycoprotein cGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV-1 envelope glycoprotein vaccine candidate. 29150937 0 76 gly glycosylated 64:75 arg1 an extensively glycosylated, trimeric HIV-1 envelope glycoprotein an extensively glycosylated, trimeric HIV-1 envelope glycoprotein Fterm glycoprotein cGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV-1 envelope glycoprotein vaccine candidate. 24872420 3 56 part_of V1/V2 499:503 arg1 the V1/V2 domain V1/V2 the V1/V2 domain Cterm Site V1/V2 domain Second, multiple broadly neutralizing monoclonal antibodies to the V1/V2 domain (e.g. PG9) have been isolated from rare infected individuals, termed elite neutralizers. 26424213 2 28 gly glycopeptides 401:413 arg2 proline-rich antimicrobial glycopeptides proline-rich antimicrobial glycopeptides glycopeptides We have analyzed the effect of distal sugar and interglycosidic linkage of disaccharides on the properties of proline-rich antimicrobial glycopeptides, formaecin I and drosocin. 28371609 6 81 gly Asn297-linked 1201:1213 arg1 TPB-WT Asn297-linked GnGn-type glycan Asn297 TPB-WT Asn297-linked GnGn-type glycan Asn297 The core of PPB-WT and TPB-WT Asn297-linked GnGn-type glycan contains α1,3-fucose and β1,2-xylose, which, along with the absence of terminal galactose and sialic acid, distinguishes these plant biosimilars from human IgG. 27716795 3 73 part_of gp120 755:759 arg1 the V1 and V2 hyper-variable domains HIV-1 gp120 the V1 and V2 hyper-variable domains PUBTATOR Site HIV-1 gp120 Q14624 domains To dissect the influence of glycans on the conformation these regions, we focused on an antigenic peptide fragment from a disulfide bridge-bounded region spanning the V1 and V2 hyper-variable domains of HIV-1 gp120. 27716795 3 80 part_of region 693:698 arg1 an antigenic peptide fragment region an antigenic peptide fragment fragment To dissect the influence of glycans on the conformation these regions, we focused on an antigenic peptide fragment from a disulfide bridge-bounded region spanning the V1 and V2 hyper-variable domains of HIV-1 gp120. 24693939 4 15 gly proteins 779:786 arg1 the glycan moiety proteins the glycan moiety Fterm proteins RESULTS We have identified a novel enzyme in the biosynthesis of the glycan moiety of arabinogalactan proteins. 28672761 8 50 part_of mucin-derived 1013:1025 arg1 mucin-derived peptides mucin mucin-derived peptides PUBTATOR Site mucin 100508689 peptides The presence of GlcNAc glycosides affected the interaction of the three enzymes with mucin-derived peptides. 28276098 2 5 gly glycoprotein 415:426 arg1 a synthetic glycoprotein a synthetic glycoprotein Fterm glycoprotein In this paper, a novel partial 15 N-labeling method for the amide backbone of a synthetic glycoprotein is proposed. 27085741 3 89 gly glycoforms 827:836 arg1 various poly- and oligosaccharide glycoforms various poly- and oligosaccharide glycoforms Structural analysis usually requires degradation of LPS and further efficient separation of various poly- and oligosaccharide glycoforms. 26160171 3 2 part_of mucin-like 458:467 arg1 the mucin-like domain mucin the mucin-like domain PUBTATOR Site mucin 100508689 domain Here, we address this issue by studying a HSV-1 mutant lacking the mucin-like domain in gC and the corresponding purified mutant protein (gCΔmuc) in cell culture and GAG-binding assays, respectively. 29131232 3 63 part_of hydrolase 652:660 arg1 free peptides hydrolase free peptides Fterm Site hydrolase peptides For comparison, free peptides, graphene oxide mixed with free peptides (GO-peptdies) and self-assembled peptide nanofibers (PNFs) were also studied for their activity as a hydrolase mimetics for degradation of cellobiose. 28729422 3 51 part_of protein 461:467 arg1 small cysteine-rich protein motifs protein small cysteine-rich protein motifs Fterm Site protein motifs Epidermal growth factor-like (EGF) repeats are also small cysteine-rich protein motifs that can be O-glycosylated by several ER-localized enzymes, including protein O-glucosyltransferase 1 (POGLUT1) and POFUT1. 25151386 3 77 gly channels 375:382 arg1 The N-linked glycans channels The N-linked glycans Fterm channels The N-linked glycans of ion channels are large, contributing between 5 and 50 % of their molecular weight. 25779987 5 84 gly glycoproteins 809:821 arg1 acute-phase glycoproteins acute-phase glycoproteins Fterm glycoproteins Spectra of acute-phase glycoproteins and serum fractions were analyzed to probe the origins of the GlycA signal. 26266936 4 92 gly sialylated 603:612 arg1 sialylated structures sialylated structures Therefore, studying the effect of distinct Fc glycan species such as galactosylated and sialylated structures is challenging due to the lack of well-defined differences in glycan patterns of samples used. 25261472 6 45 gly glycosylation 966:978 arg1 EMV protein trafficking EMV protein trafficking Fterm protein In this study, we further explore the role of glycosylation in EMV protein trafficking. 29292146 6 44 part_of contains 891:898 arg1 One domain AND One domain One domain One domain domain One domain contains a RG I-type backbone with branching at O-3 of GalpA residues to terminal β-D-GlcpA residues, another similarly contains a RG I-type backbone but is branched at O-4 of the Rhap residues to terminal GalpA residues or oligosaccharides containing α-linked 4-Galp and terminal GalpA residues. 27716795 2 75 gly glycosylation 438:450 arg2 glycosylation sites glycosylation sites sites Here we explore the patterns of HIV-1 Env gp120 glycosylation, and particularly the enrichment in glycosylation sites proximal to the disulfide linkages at the base of the surface-exposed variable domains. 27031228 0 19 part_of Domain 128:133 arg1 Thr52 Domain Thr52 Fterm AminoAcid Domain Thr52 LpMab-12 Established by CasMab Technology Specifically Detects Sialylated O-Glycan on Thr52 of Platelet Aggregation-Stimulating Domain of Human Podoplanin. 27031228 0 31 part_of Podoplanin 144:153 arg1 Platelet Aggregation-Stimulating Domain Human Podoplanin Platelet Aggregation-Stimulating Domain OGER Human Podoplanin Q86YL7 LpMab-12 Established by CasMab Technology Specifically Detects Sialylated O-Glycan on Thr52 of Platelet Aggregation-Stimulating Domain of Human Podoplanin. 27226767 4 45 gly glycoproteins 469:481 arg1 These glycoproteins These glycoproteins Fterm glycoproteins These glycoproteins can interact with the host to affect infection rates, and are known to perform other functions. 24872420 0 35 part_of V1/V2 28:32 arg1 V1/V2 domain V1/V2 V1/V2 domain Cterm Site V1/V2 domain HIV-1 envelope proteins and V1/V2 domain scaffolds with mannose-5 to improve the magnitude and quality of protective antibody responses to HIV-1. 24721674 7 13 gly N-glycosites 1470:1481 arg2 82 N-glycosites 82 N-glycosites N-glycosites Furthermore, 177 glycopeptides representing 82 N-glycosites with both glycan composition and peptide sequence were identified by high energy collision dissociation. 24721674 7 83 gly glycopeptides 1440:1452 arg2 177 glycopeptides 177 glycopeptides glycopeptides Furthermore, 177 glycopeptides representing 82 N-glycosites with both glycan composition and peptide sequence were identified by high energy collision dissociation. 28165356 1 58 part_of glycoprotein 190:201 arg1 three potential glycosylation sites glycoprotein three potential glycosylation sites Fterm Site glycoprotein sites Human thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the α-subunit and one in the β-subunit. 26424213 1 64 gly glycosylation 143:155 arg1 protein structure protein structure Fterm protein The effect of glycosylation on protein structure and function depends on a variety of intrinsic factors including glycan chain length. 26424213 1 97 gly factors 225:231 arg1 glycan chain length factors glycan chain length Fterm factors The effect of glycosylation on protein structure and function depends on a variety of intrinsic factors including glycan chain length. 27302155 1 0 gly glycoprotein 170:181 arg1 a glycoprotein a glycoprotein Fterm glycoprotein Immunoglobulin A (IgA) is a glycoprotein of which altered glycosylation has been associated with several pathologies. 24526361 7 21 gly bond 1448:1451 arg1 intact glycoproteins glycoproteins bond Fterm glycoproteins In doing so, we demonstrate the utility of the α-1,2/3/6-mannosidase from Jack bean in not only reducing extended O-linked mannose chains but also in specifically hydrolyzing the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins. 24526361 7 87 gly glycoproteins 1463:1475 arg1 intact glycoproteins intact glycoproteins Fterm glycoproteins In doing so, we demonstrate the utility of the α-1,2/3/6-mannosidase from Jack bean in not only reducing extended O-linked mannose chains but also in specifically hydrolyzing the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins. 28912268 5 59 gly glycosylation 798:810 arg1 GlcNAc GlcNAc Disparity in GlcNAc to ribitol connectivity, as well as variable O-acetylation and glycosylation of GlcNAc contribute to the structural diversity of WTAs. 28955895 3 73 gly glycoproteins 495:507 arg1 different genotypes HCV glycoproteins different genotypes HCV glycoproteins Fterm glycoproteins Glycosylation at certain sites of different genotypes HCV glycoproteins shows substantial differences in functions of the individual glycans (Goffard et al., 2005; Helle et al., 2010) [1], [2]. 28955895 3 93 gly Glycosylation 437:449 arg2 certain sites certain sites sites Glycosylation at certain sites of different genotypes HCV glycoproteins shows substantial differences in functions of the individual glycans (Goffard et al., 2005; Helle et al., 2010) [1], [2]. 27833622 6 27 gly glycosylated 830:841 arg1 UDP-arabinopyranose mutase (UAM)/reversible glycosylated polypeptides UDP-arabinopyranose mutase (UAM)/reversible glycosylated polypeptides polypeptides A family of UDP-arabinopyranose mutase (UAM)/reversible glycosylated polypeptides catalyze the interconversion between UDP-arabinopyranose (UDP-Arap) and UDP-Araf. 27031228 8 49 part_of hPDPN 1095:1099 arg1 The minimal epitope hPDPN The minimal epitope PUBTATOR Site hPDPN 10630 epitope The minimal epitope of LpMab-12 was identified as Asp49-Pro53 of hPDPN. 24608122 4 13 gly glycoprotein 468:479 arg1 the human glycoprotein lactoferrin the human glycoprotein lactoferrin Fterm glycoprotein In this report we identified the human glycoprotein lactoferrin (hLF) as a new substrate for EndoE. 27313224 3 65 part_of isoforms 607:614 arg1 luminal domain 4 isoforms luminal domain 4 Fterm Site isoforms domain The luminal domain 4 (LD4) of the three synaptic vesicle glycoprotein 2 (SV2) isoforms A-C mediates uptake of the clinically most relevant serotype BoNT/A1. 29202246 9 40 gly glycosylated 1240:1251 arg1 the glycosylated variants the glycosylated variants Fterm variants Through this research, we revealed that most of the glycosylated variants have decreased inflammation-associated properties and identified one particular glyco variant, a truncated RANTES containing a Galβ1-3GalNAc disaccharide α-linked to Ser4, which stands out as having the best overall properties: relatively high HIV-1 inhibition potency but also weak inflammatory properties. 29202246 9 2 gly containing 1376:1385 arg1 one particular glyco variant AND a Galβ1-3GalNAc disaccharide one particular glyco variant a Galβ1-3GalNAc disaccharide Fterm variant Through this research, we revealed that most of the glycosylated variants have decreased inflammation-associated properties and identified one particular glyco variant, a truncated RANTES containing a Galβ1-3GalNAc disaccharide α-linked to Ser4, which stands out as having the best overall properties: relatively high HIV-1 inhibition potency but also weak inflammatory properties. 26085151 11 89 gly glycoproteins 2018:2030 arg1 the HIV envelope glycoproteins the HIV envelope glycoproteins Fterm glycoproteins Increasingly, HIV bnAbs are being identified that bind to the N-linked glycans coating the HIV envelope glycoproteins gp120 and gp41, highlighting them as important targets for vaccine design. 29046357 8 30 gly glycopeptides 1270:1282 arg2 these glycopeptides these glycopeptides glycopeptides The crystal structures of PILRα complexed with these glycopeptides highlighted the importance of stereochemical positioning of the O4 atom of the sugar moiety. 26689158 9 1 gly enoxaparin 1315:1324 arg1 The disaccharide compositions enoxaparin The disaccharide compositions Fterm enoxaparin The disaccharide compositions of a heparin, two batches of the LMWH, enoxaparin, and two batches of the LMWH, nadroparin, were compared. 26689158 9 45 gly heparin 1281:1287 arg1 The disaccharide compositions heparin The disaccharide compositions Fterm heparin The disaccharide compositions of a heparin, two batches of the LMWH, enoxaparin, and two batches of the LMWH, nadroparin, were compared. 25862865 8 76 gly sialylated 1385:1394 arg1 both αvβ3 and β1-6-branched sialylated complex-type N-glycans both αvβ3 and β1-6-branched sialylated complex-type N-glycans Our results show that the acquisition of metastatic competence by melanoma cells is accompanied by alteration of αvβ3 integrin glycosylation and that both αvβ3 and β1-6-branched sialylated complex-type N-glycans promote metastatic melanoma migration on VN. 27879335 10 26 gly derived 1414:1420 arg2 pectin AND Sugars pectin Sugars Fterm pectin Sugars derived from pectin, a complex plant polysaccharide digested in the large intestine, also increased virulence gene expression. 26093517 6 58 gly glycopeptide 835:846 arg2 the glycopeptide the glycopeptide glycopeptide The MS analysis of the glycopeptide revealed that the glucose and galactose residues were nearly fully (13)C-labeled, but the mannose residues were fractionally labeled with about 20% efficiency. 25628633 0 41 gly glycoprotein 105:116 arg1 recombinant glycoprotein GA733-FcK recombinant glycoprotein GA733-FcK Fterm glycoprotein Effect of the developmental stage and tissue position on the expression and glycosylation of recombinant glycoprotein GA733-FcK in transgenic plants. 27662881 6 76 gly glycosylation 1596:1608 arg2 different glycosylation sites different glycosylation sites sites The twoplex quantitative CE-MS glycan analysis platform is ideally suited for comparability assessment of biopharmaceuticals, such as monoclonal antibodies, for differential glycomic analysis of clinical material for potential biomarker discovery or for quantitative microheterogeneity analysis of different glycosylation sites within a glycoprotein. 27662881 6 103 gly glycoprotein 1625:1636 arg1 a glycoprotein a glycoprotein Fterm glycoprotein The twoplex quantitative CE-MS glycan analysis platform is ideally suited for comparability assessment of biopharmaceuticals, such as monoclonal antibodies, for differential glycomic analysis of clinical material for potential biomarker discovery or for quantitative microheterogeneity analysis of different glycosylation sites within a glycoprotein. 26587964 3 48 gly glycoproteins 666:678 arg1 the glycoproteins the glycoproteins Fterm structure of the glycoproteins We were interested to know how two different types of oligosaccharides could influence the folding pathway or the final three-dimensional structure of the glycoproteins. 27302155 0 31 gly O-glycopeptides 125:139 arg2 O-glycopeptides O-glycopeptides O-glycopeptides Longitudinal monitoring of immunoglobulin A glycosylation during pregnancy by simultaneous MALDI-FTICR-MS analysis of N- and O-glycopeptides. 25900422 9 21 part_of EuP-82 1039:1044 arg1 the N-terminal amino acid sequences EuP-82 the N-terminal amino acid sequences Cterm Site EuP-82 sequences Based on the N-terminal amino acid sequences and peptide mass fingerprint (PMF) of EuP-82, the enzyme was identified as a new serine protease. 28019699 1 11 gly glycoprotein 215:226 arg1 an important transmembrane glycoprotein an important transmembrane glycoprotein Fterm glycoprotein The epidermal growth factor receptor (EGFR) is an important transmembrane glycoprotein kinase involved the initiation or perpetuation of signal transduction cascades within cells. 27031228 9 61 part_of hPDPN 1167:1171 arg1 the synthetic glycopeptide hPDPN the synthetic glycopeptide PUBTATOR Site hPDPN 10630 glycopeptide Furthermore, LpMab-12 reacted with the synthetic glycopeptide of hPDPN, corresponding to 38-54 amino acids (hpp3854: 38-EGGVAMPGAEDDVVTPG-54), which carries α2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52. 28554385 1 59 part_of has 243:245 arg1 this glycoprotein AND three N-glycosylation sites this glycoprotein three N-glycosylation sites Fterm Site glycoprotein sites Terminal sialic acids on N-glycan of recombinant human erythropoietin are very important for in vivo half-life, as this glycoprotein has three N-glycosylation sites. 24789815 5 49 gly glycoprotein 1099:1110 arg1 glycoprotein glycans glycoprotein glycans The developed method is based on the NMR structural-reporter-group concept earlier established for glycoprotein glycans. 25199692 2 15 gly N-glycosylation 244:258 arg1 the IgG1 Fc domain FcγR domain Cterm FcγR domain N-glycosylation of the IgG1 Fc domain is required for FcγR binding, though it is unclear why. 24085812 4 30 gly glycoproteins 574:586 arg1 the aberrant and selective fucosylated glycoproteins the aberrant and selective fucosylated glycoproteins Fterm glycoproteins In this study, we aimed to identify and validate the aberrant and selective fucosylated glycoproteins in the sera of patients with SCLC. 24085812 4 105 gly fucosylated 562:572 arg1 the aberrant and selective fucosylated glycoproteins the aberrant and selective fucosylated glycoproteins Fterm glycoproteins In this study, we aimed to identify and validate the aberrant and selective fucosylated glycoproteins in the sera of patients with SCLC. 29220650 1 3 gly glycosylation 262:274 arg2 the site the site site The recent paper by Stadlmann et al. (2017) provides a novel algorithm for glycoproteomics in which complex glycopeptides can be identified in complex mixtures to aid in characterizing both the site of glycosylation and the glycan structure. 29220650 1 5 gly glycopeptides 168:180 arg2 complex glycopeptides complex glycopeptides glycopeptides The recent paper by Stadlmann et al. (2017) provides a novel algorithm for glycoproteomics in which complex glycopeptides can be identified in complex mixtures to aid in characterizing both the site of glycosylation and the glycan structure. 28955895 0 90 gly N-glycosylation 31:45 arg1 viral genotype 1b proteins proteins sites Fterm proteins sites Hepatitis C virus: The role of N-glycosylation sites of viral genotype 1b proteins for formation of viral particles in insect and mammalian cells. 28471203 3 122 gly glycoprotein 410:421 arg1 the membrane glycoprotein Cancer Antigen 125 the membrane glycoprotein Cancer Antigen 125 Fterm glycoprotein Currently, the most common diagnostic biomarkers are the membrane glycoprotein Cancer Antigen 125 (CA 125), the Human Epididymal Protein 4 (HE4) and the Carcinoembryonic Antigen (CAE). 28873985 11 41 gly glycosylation 1408:1420 arg2 several inserted glycosylation sites several inserted glycosylation sites sites However, a variant with several inserted glycosylation sites near the CBM also showed lower affinity for the substrate compared to the wild-type, and we suggest that substrate interactions of the glycans depend on their exact location as well as other factors such as changes in structure and dynamics of the linker peptide. 28444931 8 13 gly glycopeptide 1010:1021 arg2 glycopeptide retention time windows glycopeptide retention time windows glycopeptide These relative retention times were used for calculation of upper and lower limits of glycopeptide retention time windows under the reversed phase conditions. 26751696 10 2 gly glycosylated 1990:2001 arg1 functionally glycosylated α-dystroglycan functionally glycosylated α-dystroglycan These data suggest that functionally glycosylated α-dystroglycan has a unique role in muscle regeneration and may influence fiber type specification post-injury. 28325309 6 46 gly utilized 991:998 arg2 Corn husk residue Corn husk residue residue Corn husk residue can be utilized as a source of fibers for material development. 26822404 0 49 gly glycosylation 8:20 arg1 surface immune receptors surface immune receptors Fterm receptors Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana. 26822404 0 49 gly glycosylation 8:20 arg1 exported proteins exported proteins Fterm proteins Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana. 28444931 2 5 gly glycopeptide 275:286 arg2 glycopeptide analysis glycopeptide analysis glycopeptide A combination of LC with MS/MS is one of the most powerful tools for glycopeptide analysis. 24184880 3 60 gly glycosylation 612:624 arg1 commercial intravenous immunoglobulin commercial intravenous immunoglobulin Fterm immunoglobulin Switched-memory B cells were cultured for 38 days and, using liquid chromatography-mass spectrometry, we analyzed IgGs' glycosylation profiles which were then compared to the glycosylation patterns of commercial intravenous immunoglobulin (IVIG). 28076415 5 31 part_of C 1001:1001 arg1 the gp120 C terminus gp120 C the gp120 C terminus PUBTATOR Site gp120 C 3700 terminus Site-directed mutagenesis, X-ray crystallography, and negative-stain electron microscopy 3D reconstructions revealed how CAP248-2B recognizes a cleavage-dependent epitope that includes the gp120 C terminus. 28076415 5 99 part_of gp120 995:999 arg1 the gp120 C terminus gp120 C the gp120 C terminus PUBTATOR Site gp120 C 3700 terminus Site-directed mutagenesis, X-ray crystallography, and negative-stain electron microscopy 3D reconstructions revealed how CAP248-2B recognizes a cleavage-dependent epitope that includes the gp120 C terminus. 26093517 4 15 part_of containing 566:575 arg1 a peptide AND the glycosylation sequon a peptide the glycosylation sequon sequon The addition of a peptide containing the glycosylation sequon produced oligosaccharide chains attached to a structurally defined peptide. 29416597 0 32 gly glycosylation 20:32 arg2 glycosylation sites glycosylation sites sites Peptide adjacent to glycosylation sites impacts immunogenicity of glycoconjugate vaccine. 25628633 12 42 gly proteins 1636:1643 arg1 ideal glycan structure profiles proteins ideal glycan structure profiles Fterm proteins Thus, biomass-harvesting time should be optimized to obtain recombinant therapeutic proteins with ideal glycan structure profiles. 24753400 2 8 gly glycans 384:390 arg1 peptides peptides peptides Site peptides We envisioned inducing antibodies that specifically recognize O-mannose glycans on proteins and peptides. 24753400 2 8 gly glycans 384:390 arg1 proteins proteins glycans Fterm proteins We envisioned inducing antibodies that specifically recognize O-mannose glycans on proteins and peptides. 26512888 6 40 gly fucosylated 837:847 arg1 fucosylated structures fucosylated structures We further show that fucosylated structures promote CTB entry into a colonic epithelial cell line and subsequent host cell intoxication. 28844310 10 15 gly Sia-alpha2-6GalNAc 1435:1452 arg1 plasma protein protein Sia-alpha2-6GalNAc Fterm protein CONCLUSIONS The glycan structure Sia-alpha2-6GalNAc in plasma protein and ST6GALNAC2 expression in peripheral leukocytes may have utility as candidate biomarkers for the clinical diagnosis and monitoring of MDD. 24122880 4 22 gly residues 514:521 arg1 the seed coat, suggesting a more flexible seed coat that resists cracking and decortication; (2) a higher content of soluble and insoluble non-starch polysaccharide fractions in the cotyledon periphery, supporting a pectic polysaccharide mechanism comprising arabinogalacturonan, homogalacturonan, rhamnogalalcturonan, and glucuronan backbone structures; (3) higher glucose and mannose residues in the cotyledon periphery, supporting a lectin-mediated mechanism of adhesion; and (4) higher arabinose residues the seed coat, suggesting a more flexible seed coat that resists cracking and decortication; (2) a higher content of soluble and insoluble non-starch polysaccharide fractions in the cotyledon periphery, supporting a pectic polysaccharide mechanism comprising arabinogalacturonan, homogalacturonan, rhamnogalalcturonan, and glucuronan backbone structures; (3) higher glucose and mannose residues in the cotyledon periphery, supporting a lectin-mediated mechanism of adhesion; and (4) higher arabinose residues RESULTS Difficult milling is associated with: (1) lower glucose and xylose residues (less cellulose and xyloglucans) and more arabinose, rhamnose and uronic acid in the seed coat, suggesting a more flexible seed coat that resists cracking and decortication; (2) a higher content of soluble and insoluble non-starch polysaccharide fractions in the cotyledon periphery, supporting a pectic polysaccharide mechanism comprising arabinogalacturonan, homogalacturonan, rhamnogalalcturonan, and glucuronan backbone structures; (3) higher glucose and mannose residues in the cotyledon periphery, supporting a lectin-mediated mechanism of adhesion; and (4) higher arabinose and glucose residues in the cotyledon periphery, supporting a mechanism involving arabinogalactan-proteins. 24980207 4 15 gly attached 569:576 arg1 seven asparagine residues AND N-linked glycans seven asparagine residues N-linked glycans asparagine residues Extensive glycosylation was observed with N-linked glycans attached to seven asparagine residues. 25199692 3 21 part_of FcγR 405:408 arg1 the FcγR polypeptide FcγR the FcγR polypeptide Cterm Site FcγR polypeptide Structures of the FcγR:Fc complex fail to explain this because the FcγR polypeptide does not bind the N-glycan. 24299891 4 25 gly used 950:953 arg2 similar compositions similar compositions positions, Hemicellulosic fractions isolated from the water-immersed samples were obtained in high yields and exhibited similar compositions, which can be used as raw materials for production of value-added products. 25782436 1 7 gly protein 251:257 arg1 sialic acid content protein sialic acid content Fterm protein OBJECTIVE To develop a rapid process analytical technology (PAT) tool that can measure sialic acid content of an Fc-fusion protein from cell culture samples. 26822404 11 3 gly structures 1919:1928 arg1 the receptor receptor structures Fterm receptor Endoglycosidase H-insensitivity of flg22 receptor, FLS2, in the cce2/cce3 mutants suggests altered glycan structures in the receptor. 24796651 8 53 gly glycopeptide 1555:1566 arg2 the glycopeptide backbone the glycopeptide backbone glycopeptide Moreover, it is a viable alternative to deduce the glycopeptide backbone particularly in cases when the peptide backbone cannot be identified by ETD/HCD. 26483551 8 58 part_of α5 1527:1528 arg1 the Calf-1,2 domain integrin α5 the Calf-1,2 domain PUBTATOR Site integrin α5 281873 domain Furthermore, consistent restoration of those N-glycans on the Calf-1,2 domain of integrin α5 reinstated the inhibitory effects as well as the complex formation with EGFR. 27279911 6 23 gly fucosylation 859:870 arg1 PSA N-glycans PSA N-glycans In the present work, we have established two methodologies to analyze the core fucosylation and the sialic acid linkage of PSA N-glycans in serum samples from BPH (29) and PCa (44) patients with different degrees of aggressiveness. 25726649 0 37 gly cordycepin 84:93 arg1 monosaccharide composition cordycepin monosaccharide composition Fterm cordycepin [Study on monosaccharide composition of intracellular polysacchride and contents of cordycepin and cordyceps polysacchride produced by Cordyceps militaris induced by blue light]. 28407380 9 50 gly glycoproteins 1845:1857 arg1 glycoproteins glycoproteins Fterm glycoproteins Furthermore, this technology can be used to generate stable plant mutant lines for biopharmaceutical production of glycoproteins displaying relative homogeneous and mammalian-like N-glycosylation features. 26559536 3 34 gly sialylated 548:557 arg1 multi-branched and highly sialylated N-glycans multi-branched and highly sialylated N-glycans The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto- and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. 24753400 0 0 part_of O-mannosyl 86:95 arg1 O-mannosyl glycopeptides O-mannosyl O-mannosyl glycopeptides Cterm Site O-mannosyl glycopeptides Synthesis of a glycopeptide vaccine conjugate for induction of antibodies recognizing O-mannosyl glycopeptides. 29098336 10 19 gly composition 1681:1691 arg1 the most abundant disaccharide position the most abundant disaccharide position Analysis of HS extracted from all cell lines tested in this study revealed a significant variation in their composition with the most abundant disaccharide being the non-sulphated ∆UA-GlcNAc. 27584569 2 14 gly sialylated 231:240 arg1 Most N-linked and O-linked glycans Most N-linked and O-linked glycans Most N-linked and O-linked glycans on VWF are sialylated. 27211674 3 41 gly residues 526:533 arg1 sugar composition residues sugar composition residues Distinct differences in the yields of alcohol insoluble residues, degree of methoxylation (DM), sugar composition, and molar mass distribution of non-starch polysaccharides were found between the seeds coat and cotyledons. 24721674 3 52 gly N-glycosylation 796:810 arg2 protein N-glycosylation sites protein N-glycosylation sites sites In this study, a systematic approach was developed which combined: an integrated hydrophilic interaction chromatography solid phase interaction (HILIC SPE) for simultaneous detergent removal and glycopeptide enrichment, and mass spectrometric identification of both protein N-glycosylation sites and site-specific glycan composition. 24721674 3 71 gly glycopeptide 717:728 arg2 glycopeptide enrichment glycopeptide enrichment glycopeptide In this study, a systematic approach was developed which combined: an integrated hydrophilic interaction chromatography solid phase interaction (HILIC SPE) for simultaneous detergent removal and glycopeptide enrichment, and mass spectrometric identification of both protein N-glycosylation sites and site-specific glycan composition. 25548871 2 46 part_of [CEL/CS+KER 186:196 arg1 [CEL/CS+KER] composites CEL [CEL/CS+KER] composites OGER Site CEL P19835 composites A method was developed in which cellulose (CEL) and/or chitosan (CS) were added to keratin (KER) to enable [CEL/CS+KER] composites to have better mechanical strength and wider utilization. 27604475 10 21 part_of contain 1597:1603 arg1 CONCLUSIONS Tailspike proteins AND large binding sites CONCLUSIONS Tailspike proteins large binding sites Fterm Site proteins sites CONCLUSIONS Tailspike proteins contain large binding sites with precisely defined specificities and are therefore promising tools to be included in serotyping procedures as rapid serotyping agents in addition to antibodies. 28493026 0 71 gly glycoprotein 71:82 arg1 a novel immunomodulatory glycoprotein a novel immunomodulatory glycoprotein Fterm glycoprotein Structural and functional characterization of a novel immunomodulatory glycoprotein isolated from ajowan (Trachyspermum ammi L.). 26822404 10 34 gly glycosylation 1702:1714 arg1 membrane proteins membrane proteins Fterm proteins Proper protein glycosylation is required for ER/Golgi processing and trafficking of membrane proteins to the plasma membrane. 24943676 6 9 gly modified 1290:1297 arg3 the S-layer proteins AND the complete, mature glycan the S-layer proteins the complete, mature glycan Fterm proteins Despite being entrapped within the periplasm, mass spectrometry analysis revealed that the S-layer proteins were modified with the complete, mature glycan found on the secreted proteins, indicating that protein translocation and glycosylation are two independent processes. 24943676 6 43 gly found 1332:1336 arg2 the secreted proteins AND the complete, mature glycan the secreted proteins the complete, mature glycan Fterm proteins Despite being entrapped within the periplasm, mass spectrometry analysis revealed that the S-layer proteins were modified with the complete, mature glycan found on the secreted proteins, indicating that protein translocation and glycosylation are two independent processes. 24198434 1 90 gly N-glycoproteins 215:229 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins Lectin affinity chromatography (LAC) can provide a valuable front-end enrichment strategy for the study of N-glycoproteins and has been used to characterize a broad range eukaryotic N-glycoproteomes. 27582506 0 32 gly N-Glycosylation 11:25 arg1 the human cation channel the human cation channel Fterm channel Effects of N-Glycosylation of the human cation channel TRPA1 on agonist-sensitivity. 25014402 2 79 gly glutamine 406:414 arg1 glucose and glutamine concentrations glutamine glucose and glutamine concentrations glutamine Cells were cultivated under different levels of glucose and glutamine concentrations with the goal of investigating the effect of nutrient depletion levels and ammonia build up on the cell growth and the glycoprofiles of the monoclonal antibody (Mab). 26160171 2 41 part_of mucin-like 299:308 arg1 a mucin-like region mucin a mucin-like region PUBTATOR Site mucin 100508689 region gC contains a mucin-like region located near the GAG-binding site, which may affect the binding activity. 24948903 8 61 part_of sites 1402:1406 arg1 secreted proteins proteins sites Fterm Site proteins sites Moreover, we apply glycomic techniques to examine (1) the sites of N-glycosylation on secreted proteins, (2) the structures of complex N- and O-glycans, and (3) the relative abundance of complex N- and O-glycans structures in insulin responsive and insulin resistant conditions. 26096447 7 35 part_of chitosan/ECM 1316:1327 arg1 the TiO2-incorporated chitosan/ECM bilayer composite ECM the TiO2-incorporated chitosan/ECM bilayer composite OGER Site ECM Q13201 composite Overall results suggest that the TiO2-incorporated chitosan/ECM bilayer composite can be a suitable candidate as a wound dressing, with an excellent inhibition of bacterial penetration and wound healing acceleration effects. 24721674 11 25 part_of proteins 2162:2169 arg1 811 N-glycosylation sites proteins 811 N-glycosylation sites Fterm Site proteins sites Totally 811 N-glycosylation sites from 567 proteins were identified and 177 intact glycopeptides with both glycan composition and peptides sequence were characterized, which provided a solution for site-specific N-glycosylation characterization of membrane. 28371609 8 37 gly Asn297-linked 1767:1779 arg1 the Asn297-linked glycans Asn297 the Asn297-linked glycans Asn297 Nevertheless, study of the antigen-binding capacity of the biosimilars showed that absence of xylose and fucose residues in the Asn297-linked glycans does not affect the ability of the glycomodified antibodies to interact with HER2/neu positive cancer cells. 28371609 8 82 gly residues 1751:1758 arg1 the Asn297-linked glycans residues in the Asn297-linked glycans residues in Nevertheless, study of the antigen-binding capacity of the biosimilars showed that absence of xylose and fucose residues in the Asn297-linked glycans does not affect the ability of the glycomodified antibodies to interact with HER2/neu positive cancer cells. 29119999 2 103 gly glycopeptide 513:524 arg2 glycopeptide connectivity glycopeptide connectivity glycopeptide Confoundingly, the complete characterization of glycopeptide connectivity usually requires the acquisition of multiple MS/MS fragmentation spectra. 28473830 7 1 gly variants 751:758 arg1 H5Man5 variants H5Man5 Fterm variants Two variants of the H5 antigen with high mannose glycosylation (H5hm) and with low-mannose glycosylation (H5Man5) were prepared utilizing different Pichia strains. 28473830 7 1 gly variants 751:758 arg1 low-mannose glycosylation variants low-mannose glycosylation Fterm variants Two variants of the H5 antigen with high mannose glycosylation (H5hm) and with low-mannose glycosylation (H5Man5) were prepared utilizing different Pichia strains. 28473830 7 1 gly variants 751:758 arg1 high mannose glycosylation variants high mannose glycosylation Fterm variants Two variants of the H5 antigen with high mannose glycosylation (H5hm) and with low-mannose glycosylation (H5Man5) were prepared utilizing different Pichia strains. 28473830 7 11 gly glycosylation 796:808 arg1 Two variants Two variants Fterm variants Two variants of the H5 antigen with high mannose glycosylation (H5hm) and with low-mannose glycosylation (H5Man5) were prepared utilizing different Pichia strains. 28473830 7 15 gly glycosylation 838:850 arg1 Two variants Two variants Fterm variants Two variants of the H5 antigen with high mannose glycosylation (H5hm) and with low-mannose glycosylation (H5Man5) were prepared utilizing different Pichia strains. 29046357 6 51 gly glycopeptide 873:884 arg2 glycopeptide derivatives glycopeptide derivatives glycopeptide Here, we chemically synthesized glycopeptide derivatives and determined the thermodynamic parameters of their interaction with PILRα. 28358137 0 15 gly glycoprotein 59:70 arg1 a plant cell wall specific glycoprotein glycosyl transferase, ExAD a plant cell wall specific glycoprotein glycosyl transferase, ExAD Fterm glycoprotein Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD. 27222530 7 50 gly sites 1111:1115 arg1 a carbohydrate binding place sites a carbohydrate binding place sites By determining the water sites in a carbohydrate binding place during the unbinding process, we found that rupture of ligand-protein interactions induces an increase in energy barrier while ligand unbinding process takes place, whereas the entry of water molecules to the binding groove and further occupation of their corresponding water sites contributes to lowering of the energy barrier. 24664549 6 22 part_of % 920:920 arg1 20% Chia- Nutrim composite 20% Chia 20% Chia- Nutrim composite OGER Site 20% Chia Q9BZP6 composite There was a decrease in the cookie diameter, and increases in the height of cookies and dough hardness using 20% Chia- Nutrim composite. 24664549 6 66 part_of Chia- 922:926 arg1 20% Chia- Nutrim composite 20% Chia 20% Chia- Nutrim composite OGER Site 20% Chia Q9BZP6 composite There was a decrease in the cookie diameter, and increases in the height of cookies and dough hardness using 20% Chia- Nutrim composite. 24198434 7 75 gly glycoproteins 2010:2022 arg1 glycoproteins glycoproteins the putative N-glycan structures Fterm glycoproteins We also demonstrated an alternative and complementary in planta recombinant expression strategy, followed by affinity MS analysis, to identify the putative N-glycan structures of glycoproteins whose abundance is too low to be readily determined by a shotgun approach, and/or combined with deglycosylation for predicted deamidated sites, using a xyloglucan-specific endoglucanase inhibitor protein as an example. 24872420 8 15 gly glycopeptide 1429:1440 arg2 glycopeptide glycopeptide glycopeptide We also describe the properties of glycopeptide scaffolds from the V1/V2 domain also expressed with mannose-5 glycans. 26822404 12 37 gly glycosylation 1965:1977 arg1 other exported proteins other exported proteins Fterm proteins CONCLUSION Proper glycosylation of MAMP receptors (or other exported proteins) is required for optimal responses to MAMPs and is important for immune signaling of host plants. 27059388 4 77 part_of 3-glucan 542:549 arg1 3-glucan composite -1, 3-glucan 3-glucan composite PUBTATOR Site -1, 3-glucan 28888 composite Such surprising phenomenon was observed for hydroxyapatite/β-1, 3-glucan composite during alveolus extraction socket augmentation in people. 28955895 0 77 part_of proteins 74:81 arg1 N-glycosylation sites proteins N-glycosylation sites Fterm Site proteins sites Hepatitis C virus: The role of N-glycosylation sites of viral genotype 1b proteins for formation of viral particles in insect and mammalian cells. 28444931 7 73 gly glycopeptides 866:878 arg2 glycopeptides glycopeptides glycopeptides Retention times of glycopeptides were expressed as relative retention times. 28955895 6 85 gly N-glycosylation 942:956 arg2 the protein N-glycosylation sites HCV sites Cterm HCV sites Point mutations were introduced in the protein N-glycosylation sites of HCV (genotype 1b) and the mutant proteins were analyzed using baculovirus expression system in mammalian and insect cells. 28955895 6 85 gly N-glycosylation 942:956 arg1 genotype 1b 1b sites Cterm 1b sites Point mutations were introduced in the protein N-glycosylation sites of HCV (genotype 1b) and the mutant proteins were analyzed using baculovirus expression system in mammalian and insect cells. 28363541 7 15 gly ASP 1126:1128 arg1 constituent monosaccharides ASP constituent monosaccharides ASP The analysis also resulted in composition of constituent monosaccharides of ASP and co-relation analysis of uronic acids content. 28276098 4 22 gly glycoprotein 938:949 arg1 a synthetic homogeneous glycoprotein a synthetic homogeneous glycoprotein Fterm glycoprotein This idea discriminated even the same type of amino acid based on the intensities of 1 H-15 N HSQC signals, combined with classic homonuclear TOCSY and NOESY methods, thus allowing for understanding the dynamics of the local conformation of a synthetic homogeneous glycoprotein. 28371609 0 35 gly Asn297-linked 62:74 arg1 Asn297-linked Glycan Asn297 Asn297-linked Glycan Asn297 Trastuzumab and Pertuzumab Plant Biosimilars: Modification of Asn297-linked Glycan of the mAbs Produced in a Plant with Fucosyltransferase and Xylosyltransferase Gene Knockouts. 24218211 4 67 gly glycosylated 815:826 arg1 glycosylated protein glycosylated protein Fterm protein For example, 2-D HSQC detects the labeling patterns of adjacent carbon atoms and provides the enrichment of individual carbon atoms of the amino acids and glucosyl and mannosyl units present in hydrolysates of glycosylated protein. 25261472 3 50 part_of protein 488:494 arg1 their protein composition protein their protein composition Fterm Site protein position, The biological functions of EMVs are highly dependent on their protein composition, which can dictate pathogenicity. 26596085 1 33 gly strain 268:273 arg1 capsular exopolysaccharide--colanic acid strain capsular exopolysaccharide--colanic acid Fterm strain The influence of RNA polymerase (rif) mutations on the yield of capsular exopolysaccharide--colanic acid (CA) of Escherichia coli K-12 lon strain was studied. 28279966 8 16 part_of Tyr1605-Met1606 1218:1232 arg1 the Tyr1605-Met1606 ADAMTS13 proteolytic site plasmin the Tyr1605-Met1606 ADAMTS13 proteolytic site PUBTATOR Site plasmin 5340 site Although both plasmin and ADAMTS13 cleave VWF in a shear-dependent manner, plasmin does not cleave at the Tyr1605-Met1606 ADAMTS13 proteolytic site in the A2 domain. 27194101 6 22 gly Glycosylation 862:874 arg1 α-dystroglycan α-dystroglycan Glycosylation of α-dystroglycan in fibroblasts from patients with hypomorphic ISPD mutations is reduced. 24518314 6 9 gly residues 1066:1073 arg1 the oligosaccharide fraction residues in the oligosaccharide fraction residues in Mannose and galactose residues in the oligosaccharide fraction are positively related to astringency perception, probably because its presence is consequence of the degradation of polysaccharides. 26968461 3 13 gly released 880:887 arg2 glycoproteins AND the major N-glycans glycoproteins the major N-glycans Fterm glycoproteins RESULTS The structural profiles of the major N-glycans released from glycoproteins and the total expression levels of the glycans were found to be mostly similar between the brain tissues of the AD patients and those of the normal control group. 26968461 3 30 gly glycoproteins 894:906 arg1 glycoproteins glycoproteins Fterm glycoproteins RESULTS The structural profiles of the major N-glycans released from glycoproteins and the total expression levels of the glycans were found to be mostly similar between the brain tissues of the AD patients and those of the normal control group. 29306439 8 20 gly proteins 1256:1263 arg1 Ac4GlcNCyoc proteins Ac4GlcNCyoc Fterm proteins These include the complete synthesis of Ac4GlcNCyoc, immunoprecipitation of EGFP-fusion proteins to examine the Ac4GlcNCyoc modification state, and a complete section on basics, performance, as well as data analysis for FLIM-FRET microscopy. 24872420 5 47 gly positions 919:927 arg1 mannose-5 glycans positions 156 and 160 mannose-5 glycans positions 156 and 160 However, the broadly neutralizing mAb, PG9, additionally depends on mannose-5 glycans at positions 156 and 160 for binding. 26655437 8 48 gly glycoprotein 1333:1344 arg1 a specific glycoprotein a specific glycoprotein Fterm glycoprotein Therefore, glycans on placental membranes alter due to preeclampsia, but changes seen at the level of the entire N-glycome may be different from the changes detected at the level of a specific glycoprotein. 27917827 1 55 gly used 196:199 arg2 The monoclonal antibody N14 The monoclonal antibody N14 N14 The monoclonal antibody N14 is used as a detection antibody in ELISA kits for the human glycoprotein afamin, a member of the albumin family, which has recently gained interest in the capture and stabilization of Wnt signalling proteins, and for its role in metabolic syndrome and papillary thyroid carcinoma. 25900908 2 26 gly glycopeptide 360:371 arg2 macrocyclic glycopeptide synthesis macrocyclic glycopeptide synthesis glycopeptide Herein, based on the previously reported method for macrocyclic glycopeptide synthesis, two series of tyrocidine A glycosylated derivatives (1a-f and 2a-f) were synthesized and evaluated for their antibacterial activities to further study the structure and activity relationships (SAR). 28444931 10 59 gly glycopeptides 1376:1388 arg2 glycopeptides glycopeptides glycopeptides Relative retention time, as a qualitative parameter, is expected to improve LC-MS/MS characterization of glycopeptides. 24721674 9 9 gly glycopeptide 1867:1878 arg2 intact glycopeptide enrichment intact glycopeptide enrichment glycopeptide It is comprised of an integrated hydrophilic interaction chromatography solid phase extraction for the simultaneous detergent removal and intact glycopeptide enrichment. 26795079 3 43 gly glycosylation 560:572 arg1 a suitably protected, α-(2 → 4)-interlinked Kdo-disaccharide a suitably protected, α-(2 → 4)-interlinked Kdo-disaccharide The glycosylation of a suitably protected, α-(2 → 4)-interlinked Kdo-disaccharide was achieved in high yield and fair anomeric selectivity using a 4,6-O-benzylidene N-phenyltrifluoroacetimidate glucosyl donor. 28279966 0 23 part_of Factor 31:36 arg1 the A1-A2 Linker Region Plasmin Cleaves Von Willebrand Factor the A1-A2 Linker Region PUBTATOR Plasmin Cleaves Von Willebrand Factor 5340 Plasmin Cleaves Von Willebrand Factor at K1491-R1492 in the A1-A2 Linker Region in a Shear- and Glycan-Dependent Manner In Vitro. 29046357 1 50 gly glycoprotein 201:212 arg1 its envelope glycoprotein B its envelope glycoprotein B Fterm glycoprotein B Before entering host cells, herpes simplex virus-1 uses its envelope glycoprotein B to bind paired immunoglobulin-like type 2 receptor α (PILRα) on immune cells. 25498664 1 19 gly sources 150:156 arg1 the chemical composition the chemical composition the chemical composition Site position, The impact of five carbohydrate sources (glucose, maltose, galactose, sucrose, and lactose) on the chemical composition, structure, morphology, and physicochemical properties, as well as, viscosity of exopolysaccharides (EPSs) produced by Lactobacillus rhamnosus E/N was investigated. 25630448 4 3 gly R 1001:1001 arg1 -hydroxyacyl residues -hydroxyacyl residues residues The double glycosyl phosphodiester was assembled by triazolyl-tris-(pyrrolidinyl)phosphonium-assisted coupling of the β-L-Ara4N H-phosphonate to α-lactol of β(1→6) diglucosamine, pentaacylated with (R)-(3)-acyloxyacyl- and Alloc-protected (R)-(3)-hydroxyacyl residues. 28656506 10 22 gly glycosylated 1684:1695 arg1 drosocin drosocin Fterm drosocin This study provides insights about naturally occurring two different glycosylated forms of drosocin. 28803976 5 20 gly glycoproteins 859:871 arg1 glycoproteins glycoproteins Fterm glycoproteins Molecular docking simulations corroborate previous data indicating that the lectin's biological activities occur mostly through interactions with glycoproteins since the lectin interacted favorably with several N-glycans, especially those of the high-mannose type. 25594223 0 47 gly glycopeptide 41:52 arg2 a long-lasting glycopeptide a long-lasting glycopeptide glycopeptide Glycosylated enfuvirtide: a long-lasting glycopeptide with potent anti-HIV activity. 27413183 2 26 gly non-glycosylated 404:419 arg1 various non-glycosylated proteins various non-glycosylated proteins Fterm proteins In vitro, the latter interaction potently suppresses the aggregation of various non-glycosylated proteins. 28165356 1 19 gly glycoprotein 190:201 arg1 a glycoprotein a glycoprotein Fterm glycoprotein Human thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the α-subunit and one in the β-subunit. 28165356 1 39 gly glycosylation 224:236 arg2 three potential glycosylation sites three potential glycosylation sites sites Human thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the α-subunit and one in the β-subunit. 28165356 1 73 gly sites 238:242 arg1 three potential glycosylation sites three potential glycosylation sites sites Human thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the α-subunit and one in the β-subunit. 27302155 5 76 gly glycopeptide 823:834 arg1 triantennary glycoforms glycopeptide triantennary glycoforms glycopeptide Thirteen distinct glycoforms were identified for the Asn340-containing tailpiece glycopeptide, mainly of the diantennary complex type, and low amounts of triantennary glycoforms. 26503547 2 64 part_of scFv 588:591 arg1 a unique antibody fragment scFv a unique antibody fragment PUBTATOR Site scFv 652070 fragment Here we report that the single-chain variable domain (scFv) M6P-1 is a unique antibody fragment with specificity for Man6P monosaccharide that, through an array-screening approach against a number of phosphorylated N-glycans, is shown to bind mono- and diphosphorylated Man6 and Man7 glycans that contain terminal αMan6P(1 → 2)αMan(1 → 3)αMan. 28990779 5 1 gly Asn-linked 1280:1289 arg1 the Asn-linked GlcNAc moiety Asn the Asn-linked GlcNAc moiety Asn Studies on the substrate specificity revealed that the α1,6-fucosidase mutants could introduce an α1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety not only to GlcNAc-peptide but also to high-mannose and complex-type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies. 28990779 5 4 gly N-glycopeptides 1402:1416 arg2 N-glycopeptides N-glycopeptides N-glycopeptides Studies on the substrate specificity revealed that the α1,6-fucosidase mutants could introduce an α1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety not only to GlcNAc-peptide but also to high-mannose and complex-type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies. 28990779 5 91 gly N-glycoproteins 1419:1433 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins Studies on the substrate specificity revealed that the α1,6-fucosidase mutants could introduce an α1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety not only to GlcNAc-peptide but also to high-mannose and complex-type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies. 28332009 2 55 gly glycoform 554:562 arg1 the complex GnGnXF glycoform the complex GnGnXF glycoform Carbohydrate characterization of an antibody (Lo-BM2) expressed in N. tabacum BY-2 cells showed that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (>70%) of the complex GnGnXF glycoform. 25191990 3 6 part_of CS/AOX 1027:1032 arg1 the CS/AOX composites AOX the CS/AOX composites PUBTATOR Site AOX Q15067 composites Fourier transform infrared spectroscopy and the swelling ratio values in the acidic pH range confirmed the influence of the synthesis strategy on the structure of the CS/AOX composites. 28682619 5 29 gly position 1229:1236 arg1 β-(1 → 3)-linked trisaccharides position 3 β-(1 → 3)-linked trisaccharides position 3 With NIS/TfOH as the promotor, 2,6-di-tert-butyl-4-methylpyridine as the base, and a dichloromethane/toluene solvent mixture, ethyl 1-thio-β-d-glucosyl disaccharide donors having 6-O-benzyl group(s) besides tert-butyldimethylsilyl groups were efficiently coupled at -40 °C to the hydroxyl group at position 3 of glucopyranosyl acceptors to form β-(1 → 2),β-(1 → 3)-linked trisaccharides, isolated in excellent 95% yield. 25900422 3 17 gly glycosylated 374:385 arg1 a glycosylated protein a glycosylated protein Fterm protein N-glycan deglycosylation tests revealed that EuP-82 was a glycosylated protein. 25548871 13 5 part_of [CEL+CS+KER 1673:1683 arg1 the [CEL+CS+KER] composite CEL the [CEL+CS+KER] composite OGER Site CEL P19835 composite Furthermore, the fact that the [CEL+CS+KER] composite has combined properties of its components, namely, superior mechanical strength (CEL), hemostasis and bactericide (CS), and controlled drug release (KER), indicates that this novel composite can be used in ways which hitherto were not possible, e.g., as a high-performance bandage to treat chronic and ulcerous wounds. 27833622 13 20 part_of lignin 1773:1778 arg1 Cell wall lignin composition lignin Cell wall lignin composition Fterm Site lignin position Cell wall lignin composition was altered with a concurrent increase in lignin content and transcript abundance of lignin biosynthetic genes in mature tillers. 28959962 8 4 gly carry 1397:1401 arg1 proteins AND a fucosylation-dependent sugar proteins a fucosylation-dependent sugar Fterm proteins Ablation of fucosylation confers resistance to the bioweapon ricin, and we discover proteins that carry a fucosylation-dependent sugar code for ricin toxicity. 25832992 4 1 part_of contains 711:718 arg1 PNGase F AND potential N-linked glycosylation sites PNGase F potential N-linked glycosylation sites OGER Site PNGase F Q96IV0 sites PNGase F itself contains potential N-linked glycosylation sites and we found that it was N-glycosylated when PNGase F secreted from silkworm cells. 24198434 5 63 part_of sites 1326:1330 arg1 230 N-glycoproteins N-glycoproteins sites Fterm Site N-glycoproteins sites Through coupling lectin affinity chromatography with a shotgun proteomics strategy, we identified 448 putative N-glycoproteins, whereas a parallel lectin affinity chromatography plus hydrophilic interaction chromatography analysis revealed 318 putative N-glycosylation sites on 230 N-glycoproteins, of which 100 overlapped with the shotgun analysis, as well as 17 N-glycan structures. 28034823 5 38 part_of -β-d-Galp- 902:911 arg1 →3,6)-β-d-Galp-(1→ residues Galp →3,6)-β-d-Galp-(1→ residues OGER Site Galp Q9UBC7 residues The non-reducing terminal α-l-Araf-(1→ was probably attached to the O-6 position of →3,6)-β-d-Galp-(1→ residues. 25900422 0 65 gly glycosylated 38:49 arg1 a new glycosylated protease a new glycosylated protease Fterm protease Biochemical characterization of a new glycosylated protease from Euphorbia cf. lactea latex. 24359545 3 6 gly glycosylation 925:937 arg2 position 7 position 7 position 7 The synthetic strategy was based on the early introduction of a phosphotriester at position 4 of heptose followed by a regioselective opening of a 6,7-O-(1,1,3,3-tetraisopropyl-1,3-disiloxane-1,3-diyl) group allowing for a straightforward access to glycosylation at position 7. 28990779 6 70 gly N-glycoproteins 1564:1578 arg1 N-glycoproteins N-glycoproteins Fterm N-glycoproteins This discovery opens a new avenue to a wide variety of homogeneous, core-fucosylated N-glycopeptides and N-glycoproteins that are hitherto difficult to obtain for structural and functional studies. 28990779 6 73 gly N-glycopeptides 1544:1558 arg2 N-glycopeptides N-glycopeptides N-glycopeptides This discovery opens a new avenue to a wide variety of homogeneous, core-fucosylated N-glycopeptides and N-glycoproteins that are hitherto difficult to obtain for structural and functional studies. 25095410 2 43 part_of regions 466:472 arg1 DRs DRs regions OGER Site DRs Q9H307 regions The results showed that the IR peaks around 1 035, 1 051, 1 078, 1 156, 1 500, 1 511 and 1 736 cm-1had perceptible differences among DRs from different regions, indicating that different DRs containing remarkable different compositions and contents of polysaccharides, ketones and esters. 25095410 2 57 part_of containing 505:514 arg1 different DRs AND remarkable different compositions different DRs remarkable different compositions OGER Site DRs Q9H307 positions The results showed that the IR peaks around 1 035, 1 051, 1 078, 1 156, 1 500, 1 511 and 1 736 cm-1had perceptible differences among DRs from different regions, indicating that different DRs containing remarkable different compositions and contents of polysaccharides, ketones and esters. 28955895 4 42 part_of proteins 707:714 arg1 differential glycosylation sites proteins differential glycosylation sites Fterm Site proteins sites In this study, differential glycosylation sites of HCV genotype 1b envelope proteins in insect and mammalian cells was demonstrated. 27125755 6 64 part_of containing 1181:1190 arg1 an expressed GH23 family protein AND a cellulose-binding domain an expressed GH23 family protein a cellulose-binding domain Fterm Site protein domain Instead, cellulose decomposition was likely performed by an expressed GH23 family protein containing a cellulose-binding domain. 27920204 2 44 gly sialylated 214:223 arg1 sialylated glycans sialylated glycans Binding of sialylated glycans or other ligands triggers signals that inhibit or activate inflammation. 27440889 12 47 gly viruses 2305:2311 arg1 recombinant HA proteins proteins recombinant HA proteins Fterm proteins Our studies indicate that unmasking the HA2 stem N-glycans of recombinant HA proteins from H5N1 and pH1N1 viruses induced more potent neutralizing antibody titers against homologous and heterosubtypic viruses. 25151386 2 18 gly glycosylation 269:281 arg1 Transport proteins Transport proteins Fterm proteins Transport proteins undergo N-linked glycosylation which can affect expression, location, stability, and function. 25939779 2 47 gly glycopeptide 340:351 arg2 glycopeptide specificity glycopeptide specificity glycopeptide The underlying molecular basis of how the lectin domains of GalNAc-Ts contribute to glycopeptide specificity and catalysis remains unclear. 25950120 2 9 part_of pectin 330:335 arg1 chemical composition pectin chemical composition Fterm Site pectin position This has been done so in an attempt to understand the chemical composition and conformation of pectin, whilst discovering and optimising new industrial applications of the polymer. 27474680 7 42 part_of [CEL+KER 1125:1132 arg1 [CEL+KER] composites CEL [CEL+KER] composites PUBTATOR Site CEL P19835 composites Specifically, adding CEL into KER substantially improves mechanical strength of [CEL+KER] composites made from all three different sources, wool, hair and chicken feathers i.e., [CEL+wool], [CEL+hair] and [CEL+feather]. 28943358 7 50 gly epitopes 1214:1221 arg1 proteins proteins epitopes Fterm proteins Overall, cyclic-carbamate-mediated ligation is useful to study the biology of carbohydrate epitopes on proteins and on cell membranes. 28283092 2 52 gly N-glycosylation 316:330 arg1 umbilical cord plasma proteins umbilical cord plasma proteins Fterm proteins We evaluated genomic damage, cell-free DNA, N-glycosylation of umbilical cord plasma proteins (PG), and nuclear division index (NDI) as possible prognostic biomarkers of health risk in the newborns of mothers with treated pregestational diabetes (NBDM; 22 mothers), compared these parameters with those from newborns of healthy mothers (NBHM; 89 mothers), and associated the results with the mothers' lifestyle in both groups, based on a detailed questionnaire. 25746404 2 14 gly protein 359:365 arg1 polysaccharides protein polysaccharides Fterm protein We found that the contents of crude protein, polysaccharides, and macroelements were highest in mycelia, whereas effective components, including mannitol, ergosterol, adenosine, inosine, Zn, and Se, were lowest in mycelia. 29199570 0 44 gly Se-Methyl-Seleno-L-Cysteine- 32:59 arg1 Se-Methyl-Seleno-L-Cysteine- and Se-Polysaccharide-Containing Extracts Cysteine Se-Methyl-Seleno-L-Cysteine- and Se-Polysaccharide-Containing Extracts Cysteine Selective Cytotoxic Activity of Se-Methyl-Seleno-L-Cysteine- and Se-Polysaccharide-Containing Extracts from Shiitake Medicinal Mushroom, Lentinus edodes (Agaricomycetes). 25187293 3 66 part_of residues 618:625 arg1 the recombinant glycoprotein glycoprotein residues Fterm Site glycoprotein residues Plant-specific N-glycosylation patterns elaborated within the Golgi complex are a major limitation of using plants to produce biopharmaceuticals as the presence of β1,2 xylose and/or α1,3 fucose residues on the recombinant glycoprotein can render the product immunogenic if administrated parenterally. 28064042 2 21 gly glycoprotein 285:296 arg1 the N-glycan structure glycoprotein the N-glycan structure Fterm glycoprotein Since the N-glycan structure of a glycoprotein should reflect the folding state, N-glycan processing may be affected by the aglycone state. 27426470 4 56 gly polysaccharides 824:838 arg1 compression wood whereas [EtPy][Br] liquefy lignin lignin polysaccharides Fterm lignin Raman microscopic analyses revealed that [C2mim][Cl] can preferentially liquefy polysaccharides in compression wood whereas [EtPy][Br] liquefy lignin. 26342810 5 18 gly protein 700:706 arg1 Fuc-GP73 protein Fuc-GP73 Fterm protein Then, AAL ELISA assay using ELISA Index was utilized to measure fucosylation level of GP73 on its protein level (Fuc-GP73). 29279116 3 52 part_of -Gal 728:731 arg1 →3)-GlcA(1→3)-Gal(1→ with sulfated branches comprised of prevelant Gal and minor Glc, and →4)-β-GlcA(1→2)-α-Man(1→ residue 1→3)-Gal →3)-GlcA(1→3)-Gal(1→ with sulfated branches comprised of prevelant Gal and minor Glc, and →4)-β-GlcA(1→2)-α-Man(1→ residue OGER Site 1→3)-Gal P22466 residue Through analysis of aldobiouronic acids released from AGSP, monosaccharide composition comparison of AGSP and its reduced product, and methylation analysis and NMR analysis of AGSP and its desulfated derivative, the main structure residue of AGSP was determined as →3)-GlcA(1→3)-Gal(1→ with sulfated branches comprised of prevelant Gal and minor Glc, and →4)-β-GlcA(1→2)-α-Man(1→ residue was also found. 24943676 3 44 gly glycosylated 590:601 arg1 the glycosylated CTD proteins the glycosylated CTD proteins Fterm proteins T. forsythia is covered with a two-dimensional crystalline surface (S-) layer composed of the glycosylated CTD proteins TfsA and TfsB. 26483551 8 56 gly N-glycans 1482:1490 arg1 the Calf-1,2 domain the Calf-1,2 domain the Calf-1,2 domain Site domain Furthermore, consistent restoration of those N-glycans on the Calf-1,2 domain of integrin α5 reinstated the inhibitory effects as well as the complex formation with EGFR. 28754625 1 16 gly position 253:260 arg1 different monosaccharide composition position different monosaccharide composition position Four antithrombotic fucoidan fractions F1, F2, LF1, and LF2 with different monosaccharide composition, molecular weight, and degree of sulfation and sulfate position were prepared from Laminaria japonica by hot water extraction and radical degradation. 29150937 5 82 gly glycoproteins 1055:1067 arg1 envelope glycoproteins envelope glycoproteins Fterm glycoproteins Our strategy involved creating a stable CHO cell line that was adapted to serum-free culture conditions to produce envelope glycoproteins. 24531467 5 66 gly present 910:916 arg1 this protein protein present Fterm protein A structure is also proposed for the most important N-glycan present in this protein as determined by digestion with specific enzymes. 24753400 0 22 gly glycopeptides 97:109 arg2 O-mannosyl glycopeptides O-mannosyl glycopeptides glycopeptides Synthesis of a glycopeptide vaccine conjugate for induction of antibodies recognizing O-mannosyl glycopeptides. 24753400 0 42 gly glycopeptide 15:26 arg2 glycopeptide glycopeptide glycopeptide Synthesis of a glycopeptide vaccine conjugate for induction of antibodies recognizing O-mannosyl glycopeptides. 26169738 2 40 gly glycoproteins 353:365 arg1 glycoproteins glycoproteins Fterm glycoproteins Antigens used for vaccination comprise a wide variety of macromolecules including glycoproteins, lipopolysaccharides, and complex carbohydrates. 28693994 2 49 gly glycosylated 298:309 arg1 phytoferritin phytoferritin Fterm phytoferritin In this work, phytoferritin was unprecedentedly glycosylated by chitosan to fabricate ferritin-chitosan Maillard reaction products (FCMPs) (grafting degree of 26.17%, 24h, 55°C). 26795079 1 37 gly attached 359:366 arg1 position 5 AND an α-D-glucopyranose position 5 an α-D-glucopyranose position 5 The heptose-deficient inner core of the lipopolysaccharide of several pathogenic strains of the Moraxellaceae family (Moraxella, Acinetobacter) and of Bartonella henselae, respectively, comprises an α-D-glucopyranose attached to position 5 of Kdo. 26512888 5 28 part_of CTB 793:795 arg1 the CTB interaction motif CTB the CTB interaction motif PUBTATOR Site CTB 1486 motif Using a metabolically incorporated photocrosslinking sugar, we identified one CTB-binding glycoprotein and demonstrated that the glycan portion of the molecule, not the protein, provides the CTB interaction motif. 27796371 2 22 gly accumulation 340:351 arg1 the hemagglutinin globular head the hemagglutinin globular head the hemagglutinin globular head Site head A decline in virulence has been accompanied by glycan accumulation on the hemagglutinin globular head, and hemagglutinin receptor binding has changed from recognition of a broad spectrum of glycan receptors to a narrower spectrum. 24526361 4 19 gly mannosylation 698:710 arg1 recombinant proteins recombinant proteins Fterm proteins Such mannosylation of recombinant proteins is considered a key factor in immunomodulation, with mannose-specific receptors binding and promoting enhanced immune responses. 24526361 4 59 gly proteins 727:734 arg1 Such mannosylation proteins Such mannosylation Fterm proteins Such mannosylation of recombinant proteins is considered a key factor in immunomodulation, with mannose-specific receptors binding and promoting enhanced immune responses. 25320359 7 20 gly glycoproteins 1082:1094 arg1 tumor-derived glycoproteins tumor-derived glycoproteins Fterm glycoproteins RESULTS We found that IgM isoagglutinins bind blood group antigens, Tn and T glycoconjugates as well as tumor-derived glycoproteins. 25939779 3 32 gly glycopeptides 656:668 arg2 glycopeptides glycopeptides glycopeptides Here we present the first crystal structures of complexes of GalNAc-T2 with glycopeptides that together with enhanced sampling molecular dynamics simulations demonstrate a cooperative mechanism by which the lectin domain enables free acceptor sites binding of glycopeptides into the catalytic domain. 25939779 3 49 gly glycopeptides 472:484 arg2 glycopeptides glycopeptides glycopeptides Here we present the first crystal structures of complexes of GalNAc-T2 with glycopeptides that together with enhanced sampling molecular dynamics simulations demonstrate a cooperative mechanism by which the lectin domain enables free acceptor sites binding of glycopeptides into the catalytic domain. 24721674 2 48 gly glycopeptides 395:407 arg2 glycopeptides glycopeptides glycopeptides However, the effective extraction of membrane proteins, the selective isolation of glycopeptides and the mass spectrometric characterization of glycosylation are challenging with current analytical techniques. 24767036 4 15 gly O-glycopeptide 447:460 arg2 O-glycopeptide bond O-glycopeptide bond O-glycopeptide The existence of O-glycopeptide bond in LbGp1 was demonstrated by β-elimination reaction. 27034286 9 61 gly glycoproteins 1521:1533 arg1 two serum glycoproteins two serum glycoproteins Fterm glycoproteins On the other hand increased level of fucosylation in two serum glycoproteins was observed in HCV-LC and HCC patients' group using Lens culinarris agglutinin. 25498658 5 53 gly had 478:480 arg1 The polysaccharide fragments AND a lower neutral sugar content The polysaccharide fragments a lower neutral sugar content The polysaccharide fragments precipitated in lower ethanol percentages had a lower neutral sugar content and a larger molecular weight. 28342150 1 11 gly glycoproteins 248:260 arg1 functional recombinant glycoproteins functional recombinant glycoproteins Fterm glycoproteins Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. 28342150 2 4 gly sialylated 555:564 arg1 terminally sialylated complex-type N-glycans terminally sialylated complex-type N-glycans Currently, various approaches have been reported to alter N-linked glycan structures of glycoproteins derived from insects into terminally sialylated complex-type N-glycans. 28342150 2 66 gly glycoproteins 504:516 arg1 N-linked glycan structures glycoproteins N-linked glycan structures Fterm glycoproteins Currently, various approaches have been reported to alter N-linked glycan structures of glycoproteins derived from insects into terminally sialylated complex-type N-glycans. 26524514 3 42 part_of VP7 533:535 arg1 an important target region VP7 an important target region PUBTATOR Site VP7 3773131 region Comparative analysis of RVA and RVC capsid proteins showed major differences at the VP7 layer, an important target region for vaccine development due to its antigenic properties. 26756572 2 15 gly glycosylation 286:298 arg1 specific proteins specific proteins Fterm proteins In order to uncover the biological roles of this modification, imaging the glycosylation state of specific proteins within living cells would be of fundamental importance. 26169738 5 7 gly N-glycosylation 786:800 arg1 specific proteins specific proteins Fterm proteins The method described, enables to analyze the N-glycosylation of specific proteins out of a complex sample or even the total of all N-glycans contained in such a sample. 28064023 4 15 gly glycosylation 988:1000 arg2 three potential Asn-linked glycosylation sites three potential Asn-linked glycosylation sites sites The deduced primary structure of the gpGILT protein includes all of the typical features of other known GILT proteins, including an active-site motif, CXXC, a GILT signature sequence, CQHGX2ECX2NX4C, three potential Asn-linked glycosylation sites, and six other conserved cysteines. 26407005 10 102 gly glycosylated 1936:1947 arg1 the tip PilA adhesin the tip PilA adhesin Fterm adhesin Our results provide the first evidence that the tip PilA adhesin can be glycosylated, and suggest that this modification is critical for PilA stability and may potentially influence interactions with the host. 25300189 2 18 part_of endoglucanase 427:439 arg1 the conserved domains endoglucanase the conserved domains Fterm Site endoglucanase domains Although PcGH5 showed similarity with the conserved domains of a cellulase superfamily GH5, a β-glucosidase/6-phospho-β-glucosidase/β-galactosidase superfamily, and an endoglucanase, recombinant PcGH5 exhibited a β-xylosidase activity, rather than endoglucanase activity. 29046357 0 73 gly glycopeptide 66:77 arg2 glycopeptide recognition glycopeptide recognition glycopeptide Structural and thermodynamic analyses reveal critical features of glycopeptide recognition by the human PILRα immune cell receptor. 26892079 4 49 part_of SR-AI 852:856 arg1 SRCR domain SR SRCR domain PUBTATOR Site SR 338386 domain However, the role of N-glycosylation and SRCR domain of SR-AI and MARCO on oAβ internalization remains unclear. 26892079 4 65 part_of MARCO 862:866 arg1 SRCR domain MARCO SRCR domain PUBTATOR Site MARCO Q9UEW3 domain However, the role of N-glycosylation and SRCR domain of SR-AI and MARCO on oAβ internalization remains unclear. 28844310 9 66 gly alpha2-6Gal/GalNAc-sialylated 1362:1390 arg1 specific alpha2-6Gal/GalNAc-sialylated proteins specific alpha2-6Gal/GalNAc-sialylated proteins LIMITATIONS Our study samples were small and we did not identify specific alpha2-6Gal/GalNAc-sialylated proteins. 28165356 2 15 gly glycoprotein 369:380 arg1 glycoprotein characterization glycoprotein characterization Fterm glycoprotein These sites are not always occupied and occupancy is frequently neglected in glycoprotein characterization, even though it is related to folding, trafficking, initiation of inflammation and host defense, as well as congenital disorders of glycosylation (CDG). 28165356 2 29 gly occupied 319:326 arg2 These sites These sites sites These sites are not always occupied and occupancy is frequently neglected in glycoprotein characterization, even though it is related to folding, trafficking, initiation of inflammation and host defense, as well as congenital disorders of glycosylation (CDG). 24618259 0 70 part_of butyrylcholinesterase 90:110 arg1 subcellular deposition butyrylcholinesterase subcellular deposition PUBTATOR Site butyrylcholinesterase 590 position Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase in Nicotiana benthamiana. 28873985 9 4 gly glycosylation 1108:1120 arg2 four glycosylation sites four glycosylation sites sites Conversely, a substitution of four glycosylation sites near the middle of the linker reduced substrate affinity and increased maximal turnover. 26093517 0 46 gly asparagine-linked 29:45 arg1 an asparagine-linked oligosaccharide asparagine an asparagine-linked oligosaccharide asparagine Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Archaeoglobus fulgidus. 26512888 7 15 gly glycoproteins 977:989 arg1 CTB-binding fucosylated glycoproteins CTB-binding fucosylated glycoproteins Fterm glycoproteins CTB-binding fucosylated glycoproteins are present in normal human intestinal epithelia and could play a role in cholera. 26512888 7 41 gly fucosylated 965:975 arg1 CTB-binding fucosylated glycoproteins CTB-binding fucosylated glycoproteins Fterm glycoproteins CTB-binding fucosylated glycoproteins are present in normal human intestinal epithelia and could play a role in cholera. 26671321 1 3 gly parasite 271:278 arg1 The ceramide phosphoinositol glycan core parasite The ceramide phosphoinositol glycan core parasite The ceramide phosphoinositol glycan core (CPI-GC) of the lipophosphoglycan of Trichomonas vaginalis is a major virulent factor of this common genitourinary parasite. 24574058 8 73 part_of MS/MS 1748:1752 arg1 diagnostic negative-mode MS/MS fragments MS/MS diagnostic negative-mode MS/MS fragments Cterm Site MS/MS fragments Furthermore, our chosen analytical approach enabled us, via observation of diagnostic negative-mode MS/MS fragments, to determine the fine structure of the methylphosphorylated and sulfated N-glycans of the M31 glucosidase mutant in their native state. 25092905 4 0 gly glycosites 861:870 arg2 glycosites glycosites glycosites Although the capacity of CHO for biosynthesis of glycan structures (glycostructures) on glycoproteins are well established, our knowledge of the capacity of CHO cells for attaching GalNAc-type O-glycans to proteins (glycosites) is minimal. 25092905 4 6 gly glycoproteins 733:745 arg1 glycoproteins glycoproteins Fterm glycoproteins Although the capacity of CHO for biosynthesis of glycan structures (glycostructures) on glycoproteins are well established, our knowledge of the capacity of CHO cells for attaching GalNAc-type O-glycans to proteins (glycosites) is minimal. 27313224 11 13 part_of SV2C-LD4 1906:1913 arg1 the SV2C-LD4 peptide SV2C the SV2C-LD4 peptide PUBTATOR Site SV2C 22987 peptide In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding a third anchor point beside a ganglioside and the SV2C-LD4 peptide, for BoNT/A neuronal cell surface binding and uptake. 25722146 0 40 gly composition 72:82 arg1 the polysaccharide position the polysaccharide position Effect of different aging techniques on the polysaccharide and phenolic composition and sensory characteristics of Syrah red wines fermented using different yeast strains. 25726649 1 127 gly cordycepin 309:318 arg1 monosaccharide composition cordycepin monosaccharide composition Fterm cordycepin OBJECTIVE To study effects of blue light irradiation on monosaccharide composition of intracellular polysacchride and contents of cordycepin and cordyceps polysacchride of mycelium and sporocarp in Cordyceps militaris. 28188862 16 61 gly sialylated 2747:2756 arg1 sialylated structures sialylated structures In addition, a correlation was established between ascites volume and degree of sialylation, showing that the high-volume ascites contains a higher amount of sialylated structures than the low-volume ascites. 29108953 10 63 part_of Gdt1p 1708:1712 arg1 the cation binding sites Gdt1p the cation binding sites PUBTATOR Site Gdt1p 852485 sites Finally, this study demonstrated that the aspartic residues of the two conserved motifs E-x-G-D-[KR], likely constituting the cation binding sites of Gdt1p, play a crucial role in Golgi glycosylation and hence in Mn2+/Ca2+transport. 26764011 5 7 gly 293O-glycoproteins 736:753 arg1 the 293O-glycoproteins the 293O-glycoproteins Fterm 293O-glycoproteins In this study, we focus on the 293O-glycoproteins (over 1900 glycosylation sites identified by ETD-MS/MS) that enter the secretory pathway and are targets of ER-localized proteinO-mannosyltransferases. 26764011 5 71 gly glycosylation 766:778 arg2 1900 glycosylation sites 1900 glycosylation sites sites In this study, we focus on the 293O-glycoproteins (over 1900 glycosylation sites identified by ETD-MS/MS) that enter the secretory pathway and are targets of ER-localized proteinO-mannosyltransferases. 27492264 2 4 part_of IgG 344:346 arg1 the IgG Fc region IgG the IgG Fc region Cterm Site IgG region The destruction of IgG-coated targets by cell-mediated pathways begins with an interaction between the IgG Fc region and multiple varieties of membrane-bound Fc γ receptors (FcγRs) on the surface of leukocytes. 27492264 2 35 part_of receptors 404:412 arg1 the IgG Fc region receptors the IgG Fc region Fterm Site receptors region The destruction of IgG-coated targets by cell-mediated pathways begins with an interaction between the IgG Fc region and multiple varieties of membrane-bound Fc γ receptors (FcγRs) on the surface of leukocytes. 28990779 4 77 part_of glycoproteins 1072:1084 arg1 complex N-glycopeptides glycoproteins complex N-glycopeptides Fterm Site glycoproteins N-glycopeptides We found that mutation at the nucleophilic residue (D200) did not provide a typical glycosynthase from this bacterial enzyme, but several mutants with mutation at the general acid/base residue E274 of the Lactobacillus casei α1,6-fucosidase, including E274A, E274S, and E274G, acted as efficient glycoligases that could fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using α-fucosyl fluoride as a simple donor substrate. 27440889 10 55 part_of proteins 1999:2006 arg1 the stem regions proteins the stem regions Fterm Site proteins regions N-linked glycosylation sites in the stem regions of influenza virus hemagglutinin (HA) proteins are mostly well conserved among various influenza virus strains. 28457468 5 36 gly used 740:743 arg2 the cell wall residue the cell wall residue residue The amounts of glucose released by Accellerase-1500® treatment of the cell wall and the cell wall residue remaining after each extraction were used to assess the roles of pectin and hemicellulose in the recalcitrance of Sida biomass. 28358137 1 49 gly glycoproteins 130:142 arg1 plant cell wall glycoproteins plant cell wall glycoproteins Fterm glycoproteins Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. 25261472 8 39 gly glycoprotein 1258:1269 arg1 a specific EMV glycoprotein a specific EMV glycoprotein Fterm glycoprotein Furthermore, we show that genetic manipulation of the glycosylation levels of a specific EMV glycoprotein, EWI-2, directly impacts its recruitment as a function of N-linked glycan sites. 28672761 11 62 gly residues 1365:1372 arg1 ppGalNAc-T3 residues ppGalNAc-T3 residues We propose that acetylated residues on ppGalNAc-T3 function as control points for enzyme activity, and high level of GlcNAc glycosides promote a synergistic regulatory mechanism, leading to a metabolically disordered state. 26641950 2 80 gly glycoproteins 453:465 arg1 such proteins such proteins Fterm proteins High resolution separation and mass spectrometry (MS) analysis of intact proteoforms can contribute significantly to the characterization of such proteins, many of which are glycoproteins. 26641950 2 80 gly glycoproteins 453:465 arg1 glycoproteins glycoproteins Fterm glycoproteins High resolution separation and mass spectrometry (MS) analysis of intact proteoforms can contribute significantly to the characterization of such proteins, many of which are glycoproteins. 28636500 2 46 part_of immunoglobulin 338:351 arg1 the hinge region IgA1 immunoglobulin the hinge region PUBTATOR Site IgA1 immunoglobulin P01876 region In the serum of patients with IgAN, the hinge region of IgA1 immunoglobulin contains aberrantly glycosylated O-glycans deficient in galactose, which is normally added to the core 1 O-glycan structure by core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1), the key enzyme in the process of glycosylation. 28636500 2 36 part_of contains 353:360 arg1 the hinge region AND the hinge region the hinge region the hinge region region In the serum of patients with IgAN, the hinge region of IgA1 immunoglobulin contains aberrantly glycosylated O-glycans deficient in galactose, which is normally added to the core 1 O-glycan structure by core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1), the key enzyme in the process of glycosylation. 27524389 2 94 gly heterogeneity 328:340 arg1 carbohydrates carbohydrates However, studying these interactions is challenging because of the complexity and heterogeneity of the cell surface, the inherent structural complexity of carbohydrates, and the typically weak affinities of the binding reactions between the lectins and monovalent carbohydrates. 27920204 9 16 part_of Siglec-E 1335:1342 arg1 two Siglec-E polypeptides Siglec-E two Siglec-E polypeptides PUBTATOR Site Siglec-E 83382 polypeptides Homology-based structural modeling predicted a cysteine residue (Cys-298) in position to form a disulfide bridge between two Siglec-E polypeptides. 26266936 6 42 gly sialylation 1060:1070 arg1 fully galactosylated Fc glycans fully galactosylated Fc glycans This sample set comprises preparations with minimal and maximal galactosylation and different levels of sialylation of fully galactosylated Fc glycans. 29022389 3 35 gly glycoprotein 672:683 arg1 a glycoprotein mucin a glycoprotein mucin Fterm glycoprotein The aim of this study was to compare the impact of different oligo- and polysaccharides (galacto- and fructooligosaccharides, resistant starch, levan, inulin, arabinogalactan, xylan, pectin and chitin), and a glycoprotein mucin on the growth and metabolism of faecal microbiota in vitro by using isothermal microcalorimetry (IMC). 27229623 5 17 gly fucosylated 885:895 arg1 fucosylated glycans fucosylated glycans Also, the trend to higher prevalence of systemic inflammatory disorders was shown for twins with low level of fucosylated glycans and high level of non-fucosylated glycans. 27229623 5 38 gly non-fucosylated 923:937 arg1 non-fucosylated glycans non-fucosylated glycans Also, the trend to higher prevalence of systemic inflammatory disorders was shown for twins with low level of fucosylated glycans and high level of non-fucosylated glycans. 25782436 2 30 gly protein 440:446 arg1 sialic acid content protein sialic acid content Fterm protein RESULTS A statistical significant correlation between the sialic acid content and size-exclusion chromatography (SEC)-HPLC retention time of an Fc-fusion protein was observed when analyzing the titer of the samples. 26424213 0 65 gly glycopeptides 114:126 arg2 proline rich antimicrobial glycopeptides proline rich antimicrobial glycopeptides glycopeptides Effect of distal sugar and interglycosidic linkage of disaccharides on the activity of proline rich antimicrobial glycopeptides. 27302155 3 32 gly O-glycopeptides 487:501 arg2 O-glycopeptides O-glycopeptides O-glycopeptides Here we present a high-throughput strategy for the simultaneous analysis of serum-derived IgA1 N- and O-glycopeptides using matrix-assisted laser/desorption ionisation Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometry (MS). 24550397 9 47 gly glycosylation 1750:1762 arg2 sites sites sites Sequestration within microparticles may facilitate delivery of glycosylation substrate at effective dosages to sites of extracellular glycosylation while minimizing excessive dilution. 25000122 1 33 gly glycoprotein 150:161 arg1 a multiphosphorylated extracellular glycoprotein a multiphosphorylated extracellular glycoprotein Fterm glycoprotein OPN (osteopontin) is a multiphosphorylated extracellular glycoprotein, which has important roles in bone remodelling, inflammation and cancer metastasis. 26477576 6 21 gly glycosylation 1153:1165 arg2 two potential N-linked glycosylation sites two potential N-linked glycosylation sites sites The deduced protein possesses the typical structural features of known GILTs, including an active site motif, two potential N-linked glycosylation sites, a GILT signature sequence, and six conserved cysteines. 28109810 8 14 part_of seaweed/SPF 1201:1211 arg1 25:75 seaweed/SPF composites SPF 25:75 seaweed/SPF composites OGER Site SPF O76054 composites Thermal stability of hybrid composites was enhanced, indicated by a higher onset degradation temperature (259°C) for 25:75 seaweed/SPF composites than the individual seaweed composites (253°C). 28959962 6 6 gly glycoproteins 932:944 arg1 experimentally confirmed glycoproteins experimentally confirmed glycoproteins Fterm glycoproteins This analysis nearly doubles the number of experimentally confirmed glycoproteins, identifies previously unknown glycosylation sites and multiple glycosylated stemness factors, and uncovers evolutionarily conserved as well as species-specific glycoproteins in embryonic stem cells. 28959962 6 19 gly glycosylation 977:989 arg2 previously unknown glycosylation sites previously unknown glycosylation sites sites This analysis nearly doubles the number of experimentally confirmed glycoproteins, identifies previously unknown glycosylation sites and multiple glycosylated stemness factors, and uncovers evolutionarily conserved as well as species-specific glycoproteins in embryonic stem cells. 28959962 6 41 gly glycosylated 1010:1021 arg1 multiple glycosylated stemness factors multiple glycosylated stemness factors Fterm factors This analysis nearly doubles the number of experimentally confirmed glycoproteins, identifies previously unknown glycosylation sites and multiple glycosylated stemness factors, and uncovers evolutionarily conserved as well as species-specific glycoproteins in embryonic stem cells. 28959962 6 63 gly glycoproteins 1107:1119 arg1 evolutionarily conserved as well as species-specific glycoproteins evolutionarily conserved as well as species-specific glycoproteins Fterm glycoproteins This analysis nearly doubles the number of experimentally confirmed glycoproteins, identifies previously unknown glycosylation sites and multiple glycosylated stemness factors, and uncovers evolutionarily conserved as well as species-specific glycoproteins in embryonic stem cells. 28656506 4 21 gly monoglycosylated 693:708 arg1 drosocin i.e. α-GalNAc-drosocin drosocin i.e. α-GalNAc-drosocin We report herein the chemical synthesis of drosocin carrying disaccharide (β-Gal(1 → 3)α-GalNAc) and comparison of its structural and functional properties with another naturally occurring monoglycosylated form of drosocin i.e. α-GalNAc-drosocin as well as with non-glycosylated drosocin. 28656506 4 26 gly non-glycosylated 766:781 arg1 non-glycosylated drosocin non-glycosylated drosocin Fterm drosocin We report herein the chemical synthesis of drosocin carrying disaccharide (β-Gal(1 → 3)α-GalNAc) and comparison of its structural and functional properties with another naturally occurring monoglycosylated form of drosocin i.e. α-GalNAc-drosocin as well as with non-glycosylated drosocin. 28656506 4 60 gly carrying 556:563 arg1 drosocin AND disaccharide drosocin disaccharide Fterm drosocin We report herein the chemical synthesis of drosocin carrying disaccharide (β-Gal(1 → 3)α-GalNAc) and comparison of its structural and functional properties with another naturally occurring monoglycosylated form of drosocin i.e. α-GalNAc-drosocin as well as with non-glycosylated drosocin. 28656506 4 60 gly carrying 556:563 arg1 drosocin AND β-Gal(1 → 3)α-GalNAc drosocin β-Gal(1 → 3)α-GalNAc Fterm drosocin We report herein the chemical synthesis of drosocin carrying disaccharide (β-Gal(1 → 3)α-GalNAc) and comparison of its structural and functional properties with another naturally occurring monoglycosylated form of drosocin i.e. α-GalNAc-drosocin as well as with non-glycosylated drosocin. 28860654 6 47 gly carry 1073:1077 arg1 C. reinhardtii proteins AND linear Man5GlcNAc2 C. reinhardtii proteins linear Man5GlcNAc2 Fterm proteins Mass spectrometry analyses combined with enzyme sequencing showed that C. reinhardtii proteins carry linear Man5GlcNAc2 instead of the branched structure usually found in eukaryotes. 26342810 8 10 gly glycoprotein 1178:1189 arg1 focused glycoprotein focused glycoprotein Fterm glycoprotein These data indicated that multiple lectin assays could contribute to pre-clinical evaluation of focused glycoprotein and Fuc-GP73 could act as a potential glycobiomarker complementary to AFP-L3 for discrimination of HCC from LC patients. 25344425 4 1 part_of ECM 685:687 arg1 composition ECM composition OGER Site ECM Q13201 position However, the roles and composition of this microbial ECM are still poorly understood. 26756572 4 31 gly glycosylation 486:498 arg1 the intracellular proteins OGT, Foxo1, p53, and Akt1 the intracellular proteins OGT, Foxo1, p53, and Akt1 Fterm proteins Herein, we demonstrate protein-specific detection of the glycosylation of the intracellular proteins OGT, Foxo1, p53, and Akt1 in living cells. 27664852 4 4 gly N-glycosylation 669:683 arg2 Asn47 Asn47 Asn47 The heavier chain contains one N-glycosylation site (Asn47) occupied with either pauci-mannose type [GlcNAc2(Fuc)Man3(Xyl)] or complex type [GlcNAc2(Fuc)Man3(Xyl)GlcNAc(Fuc)Gal] N-glycans. 27664852 4 4 gly N-glycosylation 669:683 arg2 one N-glycosylation site one N-glycosylation site site The heavier chain contains one N-glycosylation site (Asn47) occupied with either pauci-mannose type [GlcNAc2(Fuc)Man3(Xyl)] or complex type [GlcNAc2(Fuc)Man3(Xyl)GlcNAc(Fuc)Gal] N-glycans. 27031228 11 71 gly sialylation 1581:1591 arg1 Thr52 Thr52 Thr52 AminoAcid Thr52 Thus, LpMab-12 could serve as a new diagnostic tool for determining whether hPDPN possesses the sialylation on Thr52, a site-specific post-translational modification critical for the hPDPN association with CLEC-2. 28729422 3 27 gly cysteine-rich 447:459 arg1 Epidermal growth factor-like (EGF) repeats cysteine Epidermal growth factor-like (EGF) repeats cysteine Epidermal growth factor-like (EGF) repeats are also small cysteine-rich protein motifs that can be O-glycosylated by several ER-localized enzymes, including protein O-glucosyltransferase 1 (POGLUT1) and POFUT1. 28729422 3 51 gly protein 461:467 arg1 Epidermal growth factor-like (EGF) repeats protein Epidermal growth factor-like (EGF) repeats Fterm protein Epidermal growth factor-like (EGF) repeats are also small cysteine-rich protein motifs that can be O-glycosylated by several ER-localized enzymes, including protein O-glucosyltransferase 1 (POGLUT1) and POFUT1. 28729422 3 40 gly O-glycosylated 488:501 arg1 Epidermal growth factor-like (EGF) repeats motifs Epidermal growth factor-like (EGF) repeats motifs Epidermal growth factor-like (EGF) repeats are also small cysteine-rich protein motifs that can be O-glycosylated by several ER-localized enzymes, including protein O-glucosyltransferase 1 (POGLUT1) and POFUT1. 28952521 3 84 part_of chain 478:482 arg1 their constant region chain their constant region OGER Site chain 973 region IgA antibodies possess up to five N-glycosylation sites within their constant region of the heavy chain as compared to one site for IgG antibodies. 28276098 8 38 gly glycoprotein 1509:1520 arg1 a glycoprotein a glycoprotein Fterm glycoprotein This insight into oligosaccharide behavior suggests some further effects on binding affinity between a glycoprotein and its receptor. 28363873 4 17 gly glycoproteins 709:721 arg1 only high mannosylated glycoproteins only high mannosylated glycoproteins Fterm glycoproteins Mutant rice lacking GnTI produces only high mannosylated glycoproteins. 28363873 4 62 gly mannosylated 696:707 arg1 only high mannosylated glycoproteins only high mannosylated glycoproteins Fterm glycoproteins Mutant rice lacking GnTI produces only high mannosylated glycoproteins. 24796651 0 71 gly glycopeptides 137:149 arg2 intact glycopeptides intact glycopeptides glycopeptides Novel LC-MS² product dependent parallel data acquisition function and data analysis workflow for sequencing and identification of intact glycopeptides. 29119999 1 46 gly glycoproteins 450:462 arg1 glycoproteins glycoproteins Fterm glycoproteins Glycopeptide-level mass spectrometry (MS) and tandem mass spectrometry (MS/MS) analyses are commonly performed to establish site-specific protein glycosylation profiles that are of central importance to gaining structure-function insights on glycoproteins. 26342810 1 8 gly N-glycosylation 175:189 arg2 three potential N-glycosylation sites three potential N-glycosylation sites sites Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. 27796371 0 57 part_of hemagglutinin 61:73 arg1 the globular head hemagglutinin the globular head Fterm Site hemagglutinin head Glycosylation changes in the globular head of H3N2 influenza hemagglutinin modulate receptor binding without affecting virus virulence. 28034823 4 50 part_of -α-l-Rhap- 782:791 arg1 the residues Rhap-(1 the residues OGER Site Rhap-(1 residues Methylation and nuclear magnetic resonance analyses showed APS was mainly composed by the residues of →5)-α-l-Araf-(1→, →3)-β-d-Galp-(1→, →3,6)-β-d-Galp-(1→, →4)-α-d-GalAp-(1→ and →2)-α-l-Rhap-(1→ in the backbone. 26892079 5 63 gly N-glycosylation 1023:1037 arg2 dual N-glycosylation sites dual N-glycosylation sites sites RESULT: We found that oAβ internalization was diminished in the cells expressing SR-AI harboring mutations of dual N-glycosylation sites (i.e. N120Q-N143Q and N143Q-N184Q) while they were normally surface targeted. 27194101 1 29 gly glycosylation 135:147 arg1 α-dystroglycan α-dystroglycan Mutations in genes required for the glycosylation of α-dystroglycan lead to muscle and brain diseases known as dystroglycanopathies. 24721674 6 24 gly N-glycosylation 1347:1361 arg2 811 N-glycosylation sites 811 N-glycosylation sites sites 200μg total protein digest was processed using this approach, leading to the identification of 811 N-glycosylation sites from 567 proteins within two experimental replicates. 28064023 4 18 part_of proteins 870:877 arg1 three potential Asn-linked glycosylation sites GILT proteins three potential Asn-linked glycosylation sites PUBTATOR Site GILT proteins P13284 sites The deduced primary structure of the gpGILT protein includes all of the typical features of other known GILT proteins, including an active-site motif, CXXC, a GILT signature sequence, CQHGX2ECX2NX4C, three potential Asn-linked glycosylation sites, and six other conserved cysteines. 28064023 4 18 part_of proteins 870:877 arg1 an active-site motif GILT proteins an active-site motif PUBTATOR Site GILT proteins P13284 motif The deduced primary structure of the gpGILT protein includes all of the typical features of other known GILT proteins, including an active-site motif, CXXC, a GILT signature sequence, CQHGX2ECX2NX4C, three potential Asn-linked glycosylation sites, and six other conserved cysteines. 28064023 4 18 part_of proteins 870:877 arg1 a GILT signature sequence GILT proteins a GILT signature sequence PUBTATOR Site GILT proteins P13284 sequence The deduced primary structure of the gpGILT protein includes all of the typical features of other known GILT proteins, including an active-site motif, CXXC, a GILT signature sequence, CQHGX2ECX2NX4C, three potential Asn-linked glycosylation sites, and six other conserved cysteines. 28064023 4 18 part_of proteins 870:877 arg1 six other conserved cysteines GILT proteins six other conserved cysteines PUBTATOR AminoAcid GILT proteins P13284 cysteines The deduced primary structure of the gpGILT protein includes all of the typical features of other known GILT proteins, including an active-site motif, CXXC, a GILT signature sequence, CQHGX2ECX2NX4C, three potential Asn-linked glycosylation sites, and six other conserved cysteines. 28064023 4 37 part_of GILT 920:923 arg1 a GILT signature sequence GILT a GILT signature sequence PUBTATOR Site GILT P13284 sequence The deduced primary structure of the gpGILT protein includes all of the typical features of other known GILT proteins, including an active-site motif, CXXC, a GILT signature sequence, CQHGX2ECX2NX4C, three potential Asn-linked glycosylation sites, and six other conserved cysteines. 26968461 2 91 gly glycoproteins 583:595 arg1 glycoproteins glycoproteins N-glycans Fterm glycoproteins METHODS We designed a standard protocol for the glycoblotting combined with MALDI-TOFMS to perform rapid and quantitative profiling of the glycan parts of glycoproteins (N-glycans) and glycosphingolipids (GSLs) using human AD's post-mortem samples such as brain tissues (dissected cerebral cortices such as frontal, parietal, occipital, and temporal domains), serum and cerebrospinal fluid (CSF). 26968461 2 92 gly glycoproteins 583:595 arg1 the glycan parts glycoproteins the glycan parts Fterm glycoproteins METHODS We designed a standard protocol for the glycoblotting combined with MALDI-TOFMS to perform rapid and quantitative profiling of the glycan parts of glycoproteins (N-glycans) and glycosphingolipids (GSLs) using human AD's post-mortem samples such as brain tissues (dissected cerebral cortices such as frontal, parietal, occipital, and temporal domains), serum and cerebrospinal fluid (CSF). 25384980 6 65 gly glycoprotein 1108:1119 arg1 the major target glycoprotein neural cell adhesion molecule the major target glycoprotein neural cell adhesion molecule Fterm glycoprotein The amount of α1-2Fuc glycan associated with the major target glycoprotein neural cell adhesion molecule (NCAM) varied in a diurnal fashion, although the mRNA and protein expression of Ncam1 did not. 26342810 1 5 part_of protein 146:152 arg1 three potential N-glycosylation sites protein three potential N-glycosylation sites Fterm Site protein sites Serum GP73 is a functional resident Golgi type II membrane protein with three potential N-glycosylation sites. 28955895 2 37 gly glycoproteins 306:318 arg1 HCV envelope glycoproteins HCV envelope glycoproteins Fterm glycoproteins HCV envelope glycoproteins are involved in the virion formation; the correct folding of these proteins plays the key role in virus infectivity. 25224288 4 28 gly used 629:632 arg2 RC-Ch-Ag and RC-Ch-Ag-G composites RC-Ch-Ag and RC-Ch-Ag-G composites composites RC-Ch-Ag and RC-Ch-Ag-G composites were used as wound dressing materials in experimental wounds of rats. 27561539 5 19 part_of TBP-II 633:638 arg1 chemical composition TBP chemical composition OGER Site TBP P20226 position The results revealed that chemical composition and structural characteristic of TBP-II was mainly consisted of galactose, arabinose, xylose and glucose with a molar ratio of 0.7:1:6.3:74.2. 28279966 3 6 part_of metalloproteinase 487:503 arg1 thrombospondin type 1 motif metalloproteinase thrombospondin type 1 motif Fterm Site metalloproteinase motif Interestingly, plasmin has been successfully used as an alternative to ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif) in a mouse model of thrombotic thrombocytopenic purpura. 28279966 3 30 part_of disintegrin 471:481 arg1 thrombospondin type 1 motif ADAMTS13 thrombospondin type 1 motif PUBTATOR Site ADAMTS13 279028 motif Interestingly, plasmin has been successfully used as an alternative to ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif) in a mouse model of thrombotic thrombocytopenic purpura. 24198434 5 24 gly N-glycoproteins 1168:1182 arg1 448 putative N-glycoproteins 448 putative N-glycoproteins Fterm N-glycoproteins Through coupling lectin affinity chromatography with a shotgun proteomics strategy, we identified 448 putative N-glycoproteins, whereas a parallel lectin affinity chromatography plus hydrophilic interaction chromatography analysis revealed 318 putative N-glycosylation sites on 230 N-glycoproteins, of which 100 overlapped with the shotgun analysis, as well as 17 N-glycan structures. 24198434 5 48 gly N-glycoproteins 1339:1353 arg1 230 N-glycoproteins 230 N-glycoproteins Fterm N-glycoproteins Through coupling lectin affinity chromatography with a shotgun proteomics strategy, we identified 448 putative N-glycoproteins, whereas a parallel lectin affinity chromatography plus hydrophilic interaction chromatography analysis revealed 318 putative N-glycosylation sites on 230 N-glycoproteins, of which 100 overlapped with the shotgun analysis, as well as 17 N-glycan structures. 24198434 5 56 gly N-glycosylation 1310:1324 arg2 318 putative N-glycosylation sites 318 putative N-glycosylation sites sites Through coupling lectin affinity chromatography with a shotgun proteomics strategy, we identified 448 putative N-glycoproteins, whereas a parallel lectin affinity chromatography plus hydrophilic interaction chromatography analysis revealed 318 putative N-glycosylation sites on 230 N-glycoproteins, of which 100 overlapped with the shotgun analysis, as well as 17 N-glycan structures. 28363873 2 74 gly carry 308:312 arg1 the replacement enzymes AND mannose 6-phosphate residues the replacement enzymes mannose 6-phosphate residues Fterm enzymes Patients are treated with enzyme replacement therapy (ERT), in which the replacement enzymes are required to carry terminal mannose or mannose 6-phosphate residues to allow efficient uptake into target cells and tissues. 28363873 2 74 gly carry 308:312 arg1 the replacement enzymes AND terminal mannose the replacement enzymes terminal mannose Fterm enzymes Patients are treated with enzyme replacement therapy (ERT), in which the replacement enzymes are required to carry terminal mannose or mannose 6-phosphate residues to allow efficient uptake into target cells and tissues. 24693939 7 90 gly derived 1219:1225 arg2 arabinogalactan proteins AND various oligosaccharides arabinogalactan proteins various oligosaccharides Fterm proteins The recombinant AtGALT29A expressed in Nicotiana benthamiana demonstrated a galactosyltransferase activity, transferring galactose from UDP-galactose to a mixture of various oligosaccharides derived from arabinogalactan proteins. 28826211 8 93 gly N-glycosylation 1519:1533 arg2 194 and 947 unique N-glycosylation sites 194 and 947 unique N-glycosylation sites sites We identified 194 and 947 unique N-glycosylation sites from 2 μL of human serum and 0.1 mg of rat brain, respectively. 25271059 2 63 gly glycoproteins 326:338 arg1 single glycoproteins single glycoproteins Fterm glycoproteins The levels of branching, or antennarity, give rise to differential biological activities for single glycoproteins. 24796651 10 30 gly O-glycopeptide 1989:2002 arg2 O-glycopeptide analysis O-glycopeptide analysis O-glycopeptide Additional informatics can conceivably be developed to mine and integrate the rich information contained within for simultaneous N- and O-glycopeptide analysis. 27858135 3 20 gly deglycosylated 638:651 arg1 the enzymatically deglycosylated proteins the enzymatically deglycosylated proteins Fterm proteins Glycosylation sites were identified for the native and for the enzymatically deglycosylated proteins by mass spectrometry, confirming five to six of the seven predicted glycosylation sites in the NxS/T sequence context. 27858135 3 47 gly glycosylation 730:742 arg2 the seven predicted glycosylation sites the seven predicted glycosylation sites sites Glycosylation sites were identified for the native and for the enzymatically deglycosylated proteins by mass spectrometry, confirming five to six of the seven predicted glycosylation sites in the NxS/T sequence context. 26764011 8 56 gly ofN-glycosylation 1344:1360 arg2 the proximity ofN-glycosylation sites the proximity ofN-glycosylation sites sites Furthermore,O-mannosylation is impeded in the proximity ofN-glycosylation sites suggesting the interplay of these types of post-translational modifications. 26512888 4 9 gly glycoproteins 554:566 arg1 glycoproteins glycoproteins Fterm glycoproteins Relative contributions of gangliosides and glycoproteins to CTB binding depend on cell type, and CTB binds primarily to glycoproteins in colonic epithelial cell lines. 26512888 4 50 gly glycoproteins 477:489 arg1 glycoproteins glycoproteins Fterm glycoproteins Relative contributions of gangliosides and glycoproteins to CTB binding depend on cell type, and CTB binds primarily to glycoproteins in colonic epithelial cell lines. 24334764 5 96 gly glycosylated 1058:1069 arg1 cellular proteins cellular proteins Fterm proteins We also showed that intercellular cell adhesion molecule 1 and LAMP-2, two highly glycosylated cellular proteins, presented an altered migration profile on SDS-PAGE in peripheral blood mononuclear cells from CS patients. 29251719 1 5 gly glycoproteins 99:111 arg1 Vertebrate glycoproteins Vertebrate glycoproteins Fterm glycoproteins Vertebrate glycoproteins and glycolipids are synthesized in complex biosynthetic pathways localized predominantly within membrane compartments of the secretory pathway. 26764011 7 38 gly glycosylation 1189:1201 arg2 glycosylation sites glycosylation sites sites The analysis of glycosylation sites revealed thatO-mannosylation is favored in unstructured regions and β-strands. 26764011 7 65 gly thatO-mannosylation 1218:1236 arg1 unstructured regions unstructured regions regions The analysis of glycosylation sites revealed thatO-mannosylation is favored in unstructured regions and β-strands. 27917827 2 76 gly fucosylated 602:612 arg1 the α-1-6 core fucosylated complex glycan the α-1-6 core fucosylated complex glycan As a rare occurrence, the N14 Fab is N-glycosylated at Asn26L at the onset of the VL1 antigen-binding loop, with the α-1-6 core fucosylated complex glycan facing out of the L1 complementarity-determining region. 28444931 0 84 gly glycopeptides 57:69 arg2 glycopeptides glycopeptides glycopeptides Study of structure-dependent chromatographic behavior of glycopeptides using reversed phase nanoLC. 27226767 3 17 gly glycoproteins 376:388 arg1 glycoproteins glycoproteins Fterm glycoproteins The P. brasiliensis cell wall is a dynamic outer structure, composed of a network of glycoproteins and polysaccharides, such as chitin, glucan and N-glycosylated proteins. 27226767 3 50 gly N-glycosylated 438:451 arg1 N-glycosylated proteins N-glycosylated proteins Fterm proteins The P. brasiliensis cell wall is a dynamic outer structure, composed of a network of glycoproteins and polysaccharides, such as chitin, glucan and N-glycosylated proteins. 29306439 2 56 gly glycosylation 238:250 arg1 designated proteins designated proteins Fterm proteins The visualization of glycosylation states of designated proteins within living cells is of great importance for unraveling the biological roles of intracellular protein glycosylation. 27917827 4 28 part_of discrepancies 1074:1086 arg1 the hybridoma-derived sequence s the hybridoma-derived sequence PUBTATOR Site s 8636 sequence In addition, the map quality at 1.9 Å resolution was sufficient to crystallographically re-sequence the variable VL and VH domains and to detect discrepancies in the hybridoma-derived sequence. 26875935 6 6 gly β-galactosidase 1015:1029 arg1 glycan processing β-galactosidase glycan processing Fterm β-galactosidase It is possible to predict a novel and important function in glycan processing of this β-galactosidase, because various β-galactosyl linkages in N- and O-glycans exist in Golgi apparatus. 27222530 1 14 gly information 262:272 arg1 cellular receptors receptors information Fterm receptors Endogenous lectins can control critical biological responses, including cell communication, signaling, angiogenesis and immunity by decoding glycan-containing information on a variety of cellular receptors and the extracellular matrix. 24085812 12 36 gly fucosylated 1824:1834 arg1 a biantennary fucosylated glycan modification a biantennary fucosylated glycan modification The glycan structural analysis of PON1 by MS/MS identified a biantennary fucosylated glycan modification consisting of a core + 2HexNAc + 1Fuc at increased levels in the sera of patients with SCLC. 24533580 5 26 part_of EPOR 903:906 arg1 EPOR region EPOR EPOR region PUBTATOR Site EPOR 2057 region Three different chimeras, bearing these mutations in the cytosolic, EPOR region were generated; Hence, the differences in the chimera-related effects are specifically attributed to the mutations. 26266936 8 70 gly sialylation 1496:1506 arg1 IIIa receptor binding IIIa receptor binding Fterm receptor A variety of analytical assays, including Surface Plasmon Resonance and recently developed FcγR affinity chromatography, as well as an optimized cell-based ADCC assay were applied to investigate the effect of Fc galactosylation and sialylation on the in vitro FcγRI, IIa, and IIIa receptor binding and ADCC activity of IgG1. 24198434 3 19 gly N-glycosylation 789:803 arg2 particular N-glycosylation sites particular N-glycosylation sites sites A multi-lectin approach has also been reported to provide a significant benefit for the analysis of plant N-glycoproteins; however, it has yet to be determined whether certain lectins, or combinations of lectins are optimal for plant N-glycoproteome profiling; or whether specific lectins show preferential association with particular N-glycosylation sites or N-glycan structures. 24198434 3 76 gly N-glycoproteins 560:574 arg1 plant N-glycoproteins plant N-glycoproteins Fterm N-glycoproteins A multi-lectin approach has also been reported to provide a significant benefit for the analysis of plant N-glycoproteins; however, it has yet to be determined whether certain lectins, or combinations of lectins are optimal for plant N-glycoproteome profiling; or whether specific lectins show preferential association with particular N-glycosylation sites or N-glycan structures. 27743368 2 7 part_of composition 302:312 arg1 membrane proteins proteins composition Fterm Site proteins position To study composition and function of N-glycans on membrane proteins one has to have an efficient and reproducible analytical method, which includes protein extraction and analysis of glycans. 26587964 5 27 gly glycoproteins 1121:1133 arg1 the small glycoproteins the small glycoproteins Fterm glycoproteins From our analysis, we found that the two different oligosaccharides do not influence the folding pathway in vitro and the final structure of the small glycoproteins. 27213484 5 56 part_of BC-pectin 978:986 arg1 The BC-pectin composites pectin The BC-pectin composites Fterm Site pectin composites The BC-pectin composites reacted in a similar way with Al as do plant cell walls, providing insights into the effects of Al on the mechanical properties of the BC-pectin composites as cell wall analogues. 27213484 5 82 part_of BC-pectin 1134:1142 arg1 the BC-pectin composites pectin the BC-pectin composites Fterm Site pectin composites The BC-pectin composites reacted in a similar way with Al as do plant cell walls, providing insights into the effects of Al on the mechanical properties of the BC-pectin composites as cell wall analogues. 26559536 7 45 gly proteins 1173:1180 arg1 the N-glycans proteins the N-glycans Fterm proteins This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans. 24721674 1 80 gly Glycosylation 156:168 arg1 membrane proteins membrane proteins Fterm proteins Glycosylation of membrane proteins plays an important role in cellular behaviors such as cell-cell interaction, immunologic recognition and cell signaling. 29416597 5 61 gly glycosylation 747:759 arg2 the glycosylation sites the glycosylation sites sites Immunogenicity results showed that only peptides adjacent to the glycosylation sites (GS1 and GS2) promoted the generation of PS-specific IgG antibodies and contributed to PS-specific IgG subclass distribution. 28826211 5 30 gly N-glycopeptides 976:990 arg2 N-glycopeptides N-glycopeptides N-glycopeptides On the basis of hydrophilic interaction between the polar groups (amino groups and hydroxyl groups) on CSMs and glycan moieties on glycopeptides, the prepared CSMs were applied to specific capture of N-glycopeptides from standard protein digests and complex biological samples (body fluids and tissues). 28826211 5 33 gly moieties 895:902 arg1 glycopeptides glycopeptides glycopeptides Site glycopeptides On the basis of hydrophilic interaction between the polar groups (amino groups and hydroxyl groups) on CSMs and glycan moieties on glycopeptides, the prepared CSMs were applied to specific capture of N-glycopeptides from standard protein digests and complex biological samples (body fluids and tissues). 28826211 5 75 gly glycopeptides 907:919 arg2 glycopeptides glycopeptides glycopeptides On the basis of hydrophilic interaction between the polar groups (amino groups and hydroxyl groups) on CSMs and glycan moieties on glycopeptides, the prepared CSMs were applied to specific capture of N-glycopeptides from standard protein digests and complex biological samples (body fluids and tissues). 24737166 1 70 gly Glycosylation 73:85 arg1 cellular proteins cellular proteins Fterm proteins Glycosylation of cellular proteins has important impact on their stability and functional properties, and glycan structures strongly influence cell adhesion. 28378443 1 17 gly glycoprotein 132:143 arg1 the hydrophobic lysosomal glycoprotein saposin D the hydrophobic lysosomal glycoprotein saposin D Fterm glycoprotein The main glycoforms of the hydrophobic lysosomal glycoprotein saposin D (SapD) were synthesized by native chemical ligation. 27313224 8 15 gly core-fucosylated 1315:1330 arg1 The N559-glycan The N559-glycan The N559-glycan was characterised as a complex core-fucosylated type with a heterogeneity ranging up to tetra-antennary structure with bisecting N-acetylglucosamine which can establish extensive interactions with BoNT/A. 28943358 4 1 part_of E-selectin 567:576 arg1 E-selectin binding sites E-selectin E-selectin binding sites PUBTATOR Site E-selectin 20339 sites This method enabled the presentation of carbohydrate epitopes on live animal cells, as shown by the acquisition of E-selectin binding sites on mouse MC-38 cells decorated with 3-fucosyllactose or 3-fucosyl-3-sialyllactose. 24574058 7 62 gly attached 1609:1616 arg2 such slime mould proteins AND the oligosaccharides such slime mould proteins the oligosaccharides Fterm proteins As anionic N-glycans are considered to be mostly associated with lysosomal enzymes in Dictyostelium, we hypothesise that glycosidases present in the acidic compartments may act on the oligosaccharides attached to such slime mould proteins. 28743910 9 11 part_of hFg 1249:1251 arg1 The N-terminal Fg1 peptide hFg The N-terminal Fg1 peptide Cterm Site hFg 2244 peptide The N-terminal Fg1 peptide of hFg was recognized by DsA1, and priming DsA1 with Fg1 inhibited DsA1/hFg recognition. 28280963 1 66 gly glycoproteins 354:366 arg1 therapeutic glycoproteins therapeutic glycoproteins Fterm glycoproteins Determination of biological activity and its comparison with clinical behavior is important in the quality assessment of therapeutic glycoproteins. 24872420 1 0 part_of gp120 242:246 arg1 the V1/V2 domain gp120 the V1/V2 domain PUBTATOR Site gp120 3700 domain Two lines of investigation have highlighted the importance of antibodies to the V1/V2 domain of gp120 in providing protection from HIV-1 infection. 24872420 1 43 part_of V1/V2 226:230 arg1 the V1/V2 domain V1/V2 the V1/V2 domain Cterm Site V1/V2 domain Two lines of investigation have highlighted the importance of antibodies to the V1/V2 domain of gp120 in providing protection from HIV-1 infection. 27059388 9 34 part_of HAp/glucan 1422:1431 arg1 HAp/glucan composite HAp HAp/glucan composite OGER Site HAp P10826 composite Simple quantitative correction of amount of implanted composite was sufficient to control in vivo appearance of side effects, confirming that pH-related volume increase of HAp/glucan composite is not a disqualifying factor. 25187293 3 44 gly glycoprotein 646:657 arg1 the recombinant glycoprotein the recombinant glycoprotein Fterm glycoprotein Plant-specific N-glycosylation patterns elaborated within the Golgi complex are a major limitation of using plants to produce biopharmaceuticals as the presence of β1,2 xylose and/or α1,3 fucose residues on the recombinant glycoprotein can render the product immunogenic if administrated parenterally. 26407005 8 100 gly glycosylated 1588:1599 arg1 potentially glycosylated asparagine residues potentially glycosylated asparagine residues asparagine residues In silico search for potentially glycosylated asparagine residues in PilA sequence pointed to N427 and N597, which appear conserved and exposed in the close homolog RrgA from S. pneumoniae, as likely candidates. 24533580 1 41 part_of receptor 367:374 arg1 the intracellular region erythropoietin (EPO) receptor the intracellular region PUBTATOR Site erythropoietin (EPO) receptor 2057 region Primary familial and congenital polycythaemia (PFCP) is a disease characterized by increased red blood cell mass, and can be associated with mutations in the intracellular region of the erythropoietin (EPO) receptor (EPOR). 25187293 5 71 gly glycoprotein 1097:1108 arg1 a pharmaceutical glycoprotein a pharmaceutical glycoprotein Fterm glycoprotein This prompted us to investigate whether a pharmaceutical glycoprotein is targeted to PSVs using the same targeting sequences, thus avoiding the unwanted plant-Golgi-specific complex N-glycan modifications. 27463368 7 59 gly heterogeneity 1047:1059 arg1 AX and BG polysaccharides AX and BG polysaccharides This study provides a unique picture of the structural heterogeneity of AX and BG polysaccharides at the scale of the whole endosperm in a series of wheat and barley cultivars. 24366264 0 12 part_of channel-containing 98:115 arg1 channel-containing membrane microdomains channel channel-containing membrane microdomains Fterm Site channel microdomains Inhibition of cardiac pacemaker channel hHCN2 depends on intercalation of lipopolysaccharide into channel-containing membrane microdomains. 27313224 3 14 gly glycoprotein 586:597 arg1 vesicle glycoprotein 2 vesicle glycoprotein 2 Fterm glycoprotein 2 The luminal domain 4 (LD4) of the three synaptic vesicle glycoprotein 2 (SV2) isoforms A-C mediates uptake of the clinically most relevant serotype BoNT/A1. 28990779 0 67 gly N-Glycopeptides 75:89 arg1 Direct Core Fucosylation N-Glycopeptides Direct Core Fucosylation N-Glycopeptides Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins. 28990779 0 69 gly N-Glycoproteins 95:109 arg1 Direct Core Fucosylation N-Glycoproteins Direct Core Fucosylation Fterm N-Glycoproteins Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins. 28990779 0 90 gly Fucosylation 52:63 arg1 N-Glycopeptides N-Glycoproteins N-Glycopeptides Fterm N-Glycoproteins N-Glycopeptides Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins. 28280963 12 28 gly glycosylation 1893:1905 arg2 potential glycosylation amino acid residue sites potential glycosylation amino acid residue sites sites N-glycan units were constructed; moreover, EPO protein was glycosylated at potential glycosylation amino acid residue sites. 28280963 12 0 gly glycosylated 1867:1878 arg1 EPO protein EPO protein sites PUBTATOR EPO protein 2056 sites N-glycan units were constructed; moreover, EPO protein was glycosylated at potential glycosylation amino acid residue sites. 27582506 4 16 gly glycosylation 708:720 arg2 two putative N-linked glycosylation sites two putative N-linked glycosylation sites sites TRPA1 possesses two putative N-linked glycosylation sites at N747 and N753 that have not yet been studied in detail. 28488115 7 66 gly nystatin 1252:1259 arg1 the mycosaminyl sugar residue nystatin the mycosaminyl sugar residue Fterm nystatin P1 that is responsible for the transfer of a mannose to the mycosaminyl sugar residue of nystatin. 28407380 1 7 gly glycoproteins 205:217 arg1 glycoproteins glycoproteins Fterm glycoproteins Studies of protein N-glycosylation are important for answering fundamental questions on the diverse functions of glycoproteins in plant growth and development. 28955895 4 53 gly glycosylation 659:671 arg1 HCV genotype 1b envelope proteins proteins sites Fterm proteins sites In this study, differential glycosylation sites of HCV genotype 1b envelope proteins in insect and mammalian cells was demonstrated. 29119999 5 74 gly glycopeptides 1079:1091 arg2 glycopeptides glycopeptides glycopeptides Here, a previously developed method of multiplexing CID and ETD is applied to the study of glycopeptides for the first time. 23934913 6 2 part_of lysines 844:850 arg1 bPLBD1 PLBD1 lysines PUBTATOR AminoAcid PLBD1 317710 sites and lysines We identified candidate lysines by analyzing the structural and sequentially conserved N-glycosylation sites and lysines in bPLBD1 and in the homologous mouse PLBD2. 23934913 6 2 part_of lysines 844:850 arg1 the homologous mouse PLBD2 PLBD2 lysines PUBTATOR AminoAcid PLBD2 71772 sites and lysines We identified candidate lysines by analyzing the structural and sequentially conserved N-glycosylation sites and lysines in bPLBD1 and in the homologous mouse PLBD2. 23934913 6 29 part_of sites 834:838 arg1 bPLBD1 PLBD1 sites PUBTATOR AminoAcid PLBD1 317710 sites and lysines We identified candidate lysines by analyzing the structural and sequentially conserved N-glycosylation sites and lysines in bPLBD1 and in the homologous mouse PLBD2. 23934913 6 29 part_of sites 834:838 arg1 the homologous mouse PLBD2 PLBD2 sites PUBTATOR AminoAcid PLBD2 71772 sites and lysines We identified candidate lysines by analyzing the structural and sequentially conserved N-glycosylation sites and lysines in bPLBD1 and in the homologous mouse PLBD2. 26093517 4 18 gly attached 634:641 arg1 a structurally defined peptide AND oligosaccharide chains a structurally defined peptide oligosaccharide chains peptide The addition of a peptide containing the glycosylation sequon produced oligosaccharide chains attached to a structurally defined peptide. 26093517 4 77 gly glycosylation 581:593 arg2 the glycosylation sequon the glycosylation sequon sequon The addition of a peptide containing the glycosylation sequon produced oligosaccharide chains attached to a structurally defined peptide. 26975884 5 13 part_of Ca 723:724 arg1 four Ca(2+) ion binding sites Ca(2 four Ca(2+) ion binding sites OGER Site Ca(2 P00918 sites The crystal structures reveal the presence of four Ca(2+) ion binding sites, with three of these binding sites are highly conserved among Anoxybacillus α-amylases. 26975884 5 37 part_of 2+ 726:727 arg1 four Ca(2+) ion binding sites Ca(2 four Ca(2+) ion binding sites OGER Site Ca(2 P00918 sites The crystal structures reveal the presence of four Ca(2+) ion binding sites, with three of these binding sites are highly conserved among Anoxybacillus α-amylases. 27313224 6 40 gly glycosylation 1051:1063 arg1 N565 N534, N559 and N565 N534, N559 and N565 Mass spectrometric analysis of gSV2CLD-Fc demonstrates glycosylation of N534, N559 and N565, the latter two residing at the BoNT/A interface. 27313224 6 40 gly glycosylation 1051:1063 arg1 N559 N534, N559 and N565 N534, N559 and N565 Mass spectrometric analysis of gSV2CLD-Fc demonstrates glycosylation of N534, N559 and N565, the latter two residing at the BoNT/A interface. 27313224 6 40 gly glycosylation 1051:1063 arg1 N559 N534, N559 and N565 N534, N559 and N565 Mass spectrometric analysis of gSV2CLD-Fc demonstrates glycosylation of N534, N559 and N565, the latter two residing at the BoNT/A interface. 25199692 2 9 part_of IgG1 267:270 arg1 the IgG1 Fc domain IgG1 the IgG1 Fc domain OGER Site IgG1 P01857 domain N-glycosylation of the IgG1 Fc domain is required for FcγR binding, though it is unclear why. 25092905 2 53 gly O-glycoproteins 445:459 arg1 some O-glycoproteins some O-glycoproteins Fterm O-glycoproteins CHO is used for production of several O-glycoprotein therapeutics including erythropoietin, coagulation factors, and chimeric receptor IgG1-Fc-fusion proteins, however, some O-glycoproteins are not produced efficiently in CHO. 25092905 2 58 gly O-glycoprotein 309:322 arg1 several O-glycoprotein therapeutics several O-glycoprotein therapeutics Fterm O-glycoprotein CHO is used for production of several O-glycoprotein therapeutics including erythropoietin, coagulation factors, and chimeric receptor IgG1-Fc-fusion proteins, however, some O-glycoproteins are not produced efficiently in CHO. 24753400 4 24 gly glycopeptides 732:744 arg2 the corresponding glycopeptides the corresponding glycopeptides glycopeptides O-mannose glycosylated amino acid building blocks and the corresponding glycopeptides were prepared by chemical synthesis and then conjugated to an immune carrier protein. 24753400 4 27 gly glycosylated 670:681 arg1 O-mannose glycosylated amino acid building blocks O-mannose glycosylated amino acid building blocks O-mannose glycosylated amino acid building blocks and the corresponding glycopeptides were prepared by chemical synthesis and then conjugated to an immune carrier protein. 24693939 3 1 gly proteins 576:583 arg1 the glycan moiety proteins the glycan moiety Fterm proteins Importance of the variations in the glycan moiety of arabinogalactan proteins for their functions has been implicated, but its biosynthetic process is poorly understood. 28682619 7 54 gly residues 1779:1786 arg1 disaccharide repeating units residues in disaccharide repeating units residues in The developed methodology was subsequently applied in the synthesis of a multibranched hexasaccharide related to the capsular polysaccharide from Streptococcus pneumoniae type 37, which consists of a β-(1 → 3)-linked backbone and a β-(1 → 2)-linked side chain of d-glucosyl residues in disaccharide repeating units. 28682619 7 73 gly residues 1779:1786 arg1 a β-(1 → 2)-linked side chain residues in a β-(1 → 2)-linked side chain residues in The developed methodology was subsequently applied in the synthesis of a multibranched hexasaccharide related to the capsular polysaccharide from Streptococcus pneumoniae type 37, which consists of a β-(1 → 3)-linked backbone and a β-(1 → 2)-linked side chain of d-glucosyl residues in disaccharide repeating units. 27545408 6 54 part_of SCP 749:751 arg1 The amino acid composition SCP The amino acid composition OGER Site SCP D3ZH22 position The amino acid composition of SCP consisted of 12 amino acids. 25320359 6 68 gly O-glycosylated 893:906 arg1 aberrant O-glycosylated tumor proteins aberrant O-glycosylated tumor proteins Fterm proteins An anti-A antibody was used for immunoprecipitation of aberrant O-glycosylated tumor proteins and subsequent mass spectromic analysis. 28936655 1 32 gly glycosylated 128:139 arg1 highly glycosylated proteins proteins Fterm proteins The arabinogalactan proteins (AGPs) are highly glycosylated proteins, ubiquitous in plants that have been linked to numerous aspects of sexual reproduction in several plant species, including the monoecious tree species Quercus suber. 28894966 3 16 part_of lectin-like 546:556 arg1 the smallest lectin-like cyclic peptide lectin the smallest lectin-like cyclic peptide Fterm Site lectin peptide In the reported study, we have synthesized lectinomimics based on odorranalectin 1; the smallest lectin-like cyclic peptide isolated from the frog Odorrana grahami skin, and assessed the ability of these peptides to bind specific carbohydrates on molecular and cellular levels. 24333678 8 21 part_of CS 1287:1288 arg1 individual CS, CEL and B15C5 composites CS, CEL individual CS, CEL and B15C5 composites PUBTATOR Site CS, CEL P19835 composites That is, the adsorption capacity (qe values) for Cd(2+) and Zn(2+) by [CS+B15C5], [CEL+B15C5] and [CEL+CS+B15C5] composites are much higher than combined qe values of individual CS, CEL and B15C5 composites. 24333678 8 28 part_of CEL 1291:1293 arg1 individual CS, CEL and B15C5 composites CS, CEL individual CS, CEL and B15C5 composites PUBTATOR Site CS, CEL P19835 composites That is, the adsorption capacity (qe values) for Cd(2+) and Zn(2+) by [CS+B15C5], [CEL+B15C5] and [CEL+CS+B15C5] composites are much higher than combined qe values of individual CS, CEL and B15C5 composites. 24333678 8 41 part_of [CEL+CS+B15C5 1207:1219 arg1 [CEL+B15C5] and [CEL+CS+B15C5] composites CEL [CEL+B15C5] and [CEL+CS+B15C5] composites PUBTATOR Site CEL P19835 composites That is, the adsorption capacity (qe values) for Cd(2+) and Zn(2+) by [CS+B15C5], [CEL+B15C5] and [CEL+CS+B15C5] composites are much higher than combined qe values of individual CS, CEL and B15C5 composites. 26772917 6 22 gly proteins 674:681 arg1 sugars proteins sugars Fterm proteins The purified EPS contained 58.3% carbohydrates with 7.08% uronic acid containing sugars, functional groups such as sulfate (2.68%) and trace amounts of proteins (0.65%). 26093664 1 7 gly glycoprotein 171:182 arg1 a glycoprotein a glycoprotein Fterm glycoprotein Apolipoprotein C-III (ApoC-III) is a glycoprotein carrying the most common O-linked glycan structure and is abundantly present in serum, what renders it a suitable marker for analysis of O-glycosylation abnormalities. 26093664 1 39 gly carrying 184:191 arg1 a glycoprotein AND the most common O-linked glycan structure a glycoprotein the most common O-linked glycan structure Fterm glycoprotein Apolipoprotein C-III (ApoC-III) is a glycoprotein carrying the most common O-linked glycan structure and is abundantly present in serum, what renders it a suitable marker for analysis of O-glycosylation abnormalities. 27279911 4 48 gly fucosylation 450:461 arg1 PSA N-glycans PSA N-glycans In particular, changes in the extent of core fucosylation and sialylation of PSA N-glycans in PCa patients compared to healthy controls or BPH patients have been reported. 27279911 4 63 gly sialylation 467:477 arg1 PSA N-glycans PSA N-glycans In particular, changes in the extent of core fucosylation and sialylation of PSA N-glycans in PCa patients compared to healthy controls or BPH patients have been reported. 26160171 0 27 part_of Mucin-like 0:9 arg1 Mucin-like Region Mucin Mucin-like Region PUBTATOR Mucin 100508689 Mucin-like Region of Herpes Simplex Virus Type 1 Attachment Protein Glycoprotein C (gC) Modulates the Virus-Glycosaminoglycan Interaction. 28493026 6 22 gly glycoprotein 795:806 arg1 ajowan glycoprotein or Agp ajowan glycoprotein or Agp Fterm glycoprotein One of the proteins (30.7 kDa; ajowan glycoprotein or Agp) showed effective mitogenic activity towards splenocytes. 27474680 9 29 part_of [CEL+wool 1469:1477 arg1 [CEL+wool] composite CEL [CEL+wool] composite PUBTATOR Site CEL P19835 composite Conversely, [CEL+wool] composite has the weakest mechanical strength because wool has the highest α-helix content. 27440889 5 110 gly N-glycans 1005:1013 arg1 the HA2 stem region the HA2 stem region the HA2 stem region Site region Unmasking N-glycans in the HA2 stem region (H5 N484A and H1 N503A) was found to elicit more potent neutralizing antibody titers against homologous, heterologous, and heterosubtypic viruses. 29119999 9 49 gly glycopeptide 1685:1696 arg2 N-Linked glycopeptide ions N-Linked glycopeptide ions glycopeptide N-Linked glycopeptide ions ranging in molecular weight from 1.8 to 6.5 kDa were generated from four model glycoproteins that collectively encompassed paucimannosidic, high mannose, and complex types of N-glycosylation. 29119999 9 60 gly glycoproteins 1782:1794 arg1 four model glycoproteins four model glycoproteins Fterm glycoproteins N-Linked glycopeptide ions ranging in molecular weight from 1.8 to 6.5 kDa were generated from four model glycoproteins that collectively encompassed paucimannosidic, high mannose, and complex types of N-glycosylation. 27031228 2 59 gly sialylated 321:330 arg1 sialylated O-glycan sialylated O-glycan Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2) and the platelet-aggregating activity of human PDPN (hPDPN). 28943358 6 29 gly glycoproteins 982:994 arg1 glycoproteins glycoproteins Fterm glycoproteins Proteins were modified with oligosaccharides to study their role in stimulating cytokine secretion by dendritic cells, thus pointing to interactions between glycoproteins and phosphoinositide 3-kinase signaling in controlling interleukin-12, tumor necrosis factor alpha and interleukin-1β release. 25210975 5 20 gly sialylated 1003:1012 arg1 highly sialylated and fucosylated complex type N-glycans highly sialylated and fucosylated complex type N-glycans The secretomes comprised significant levels of highly sialylated and fucosylated complex type N-glycans, which were elevated in all cancer cells relative to HMEC (57.7-87.2% vs 24.9%, p < 0.0001 and 57.1-78.0% vs 38.4%, p < 0.0001-0.001, respectively). 25210975 5 25 gly fucosylated 1018:1028 arg1 highly sialylated and fucosylated complex type N-glycans highly sialylated and fucosylated complex type N-glycans The secretomes comprised significant levels of highly sialylated and fucosylated complex type N-glycans, which were elevated in all cancer cells relative to HMEC (57.7-87.2% vs 24.9%, p < 0.0001 and 57.1-78.0% vs 38.4%, p < 0.0001-0.001, respectively). 25779987 2 27 gly glycosylated 425:436 arg1 enzymatically glycosylated acute-phase proteins enzymatically glycosylated acute-phase proteins Fterm proteins One of these signals that we named GlycA originates from a subset of glycan N-acetylglucosamine residues on enzymatically glycosylated acute-phase proteins. 25779987 2 40 gly residues 399:406 arg1 enzymatically glycosylated acute-phase proteins proteins residues Fterm proteins One of these signals that we named GlycA originates from a subset of glycan N-acetylglucosamine residues on enzymatically glycosylated acute-phase proteins. 24948903 3 27 gly glycosylation 448:460 arg1 proteins proteins Fterm proteins In addition, the global elevation of the intracellular glycosylation of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc) via either genetic or pharmacological methods is sufficient to induce insulin resistance in both cultured cells and animal models. 29119999 0 23 gly N-glycopeptides 91:105 arg2 N-glycopeptides N-glycopeptides N-glycopeptides Ion mobility-resolved collision-induced dissociation and electron transfer dissociation of N-glycopeptides: gathering orthogonal connectivity information from a single mass-selected precursor ion population. 28034823 7 44 part_of APS 1024:1026 arg1 The initial decomposition APS The initial decomposition OGER Site APS P07288 position The initial decomposition of APS occurred at 172°C, and the rapidest weight loss rate of APS appeared at 320°C. Antioxidant activity assay showed the DPPH radical scavenging activity of APS was 67.5% at 1mg/mL. 27059388 0 50 part_of HAp/glucan 27:36 arg1 new HAp/glucan composite HAp new HAp/glucan composite OGER Site HAp P10826 composite Unexpected reaction of new HAp/glucan composite to environmental acidification: Defect or advantage? 24522548 8 35 gly fucosylated 1258:1268 arg1 fucosylated xyloglucans epitope fucosylated xyloglucans epitope epitope The significant differences in the pattern of occurrence of long 1,5-α-L-arabinan chain, homogalacturonan and fucosylated xyloglucans epitope were detected between the two internodes. 25320359 11 39 gly glycoproteins 1722:1734 arg1 PDAC O-GalNAc glycoproteins PDAC O-GalNAc glycoproteins CONCLUSION Our data elucidated a novel interaction of blood group IgM isoagglutinins and PDAC O-GalNAc glycoproteins that may contribute to the pathogenesis and progression of pancreatic cancer. 25735823 2 7 gly O-glycosylated 288:301 arg1 a bifunctional O-glycosylated natriuretic peptide a bifunctional O-glycosylated natriuretic peptide peptide Described herein is a bifunctional O-glycosylated natriuretic peptide, TcNPa, from Tropidechis carinatus venom and it unusually targets both NPR-A and NPR-B. 25855670 10 79 part_of ECM 1669:1671 arg1 ECM composition ECM ECM composition OGER Site ECM Q13201 position In addition, our study of Controls indicates that a functional COC can form provided all essential components are present above a minimum threshold level, and thus some variation in ECM composition does not adversely affect oocyte development, ovulation or fertilisation. 27511022 4 5 gly glycosylation 667:679 arg1 the protein the protein Fterm protein It was suggested that the protein underwent glycosylation in the process of expression, and this quite common modification for proteins expressed in the milk complicated its isolation. 29287329 5 19 part_of ASE 767:769 arg1 the chemical composition ASE the chemical composition OGER Site ASE P51690 position, Differences were found between ASE and HWE with the chemical composition, molecular weight distribution, rheological property, and antioxidant activities of the obtained polysaccharides, while the primary structure remained the same. 28821844 3 5 gly N-glycosylation 523:537 arg2 an N-glycosylation'sequon' an N-glycosylation'sequon' sequon N-glycosylation involves the attachment of an oligosaccharide to selected asparagine residues in the sequence N-X-S/T (X ≠ P), a motif known as an N-glycosylation'sequon'. 28821844 3 41 gly attachment 405:414 arg1 selected asparagine residues AND an oligosaccharide selected asparagine residues an oligosaccharide asparagine residues N-glycosylation involves the attachment of an oligosaccharide to selected asparagine residues in the sequence N-X-S/T (X ≠ P), a motif known as an N-glycosylation'sequon'. 24269685 3 3 part_of KIAA1199 721:728 arg1 a cleavable signal sequence KIAA1199 a cleavable signal sequence PUBTATOR Site KIAA1199 57214 sequence These results suggest that the N-terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199-mediated HA depolymerization. 29150961 2 22 part_of IgG 397:399 arg1 the IgG CH 2 domain IgG the IgG CH 2 domain Cterm Site IgG domain A conserved N-glycan positioned at the N-terminal region of the IgG CH 2 domain is critical in maintaining the quaternary structure of the molecule for Fcγ receptor engagement. 24721674 6 62 part_of proteins 1378:1385 arg1 811 N-glycosylation sites proteins 811 N-glycosylation sites Fterm Site proteins sites 200μg total protein digest was processed using this approach, leading to the identification of 811 N-glycosylation sites from 567 proteins within two experimental replicates. 26100547 2 49 gly glycosylated 492:503 arg1 the oligosaccharide the oligosaccharide This hydrolysis results in the same molecular structure as the oligosaccharide that is transferred to a protein to be glycosylated. 28596008 0 45 gly strain 104:109 arg1 newly isolated complex polysaccharides strain newly isolated complex polysaccharides Fterm strain Macrophage-stimulating activities of newly isolated complex polysaccharides from Parachlorella kessleri strain KNK-A001. 28826211 6 14 gly glycopeptides 1253:1265 arg2 glycopeptides enrichment glycopeptides enrichment glycopeptides The CSMs exhibited high selectivity (HRP/BSA = 1:100), good sensitivity (4.5 × 10-10 M of HRP), good recovery yield (74.9-106.4%), and high binding capacity (100 mg g-1) in glycopeptides enrichment. 28279966 10 22 gly local 1562:1566 arg1 local N-linked glycan expression K1491-R1492 local N-linked glycan expression K1491-R1492 Finally, we demonstrate that VWF susceptibility to plasmin proteolysis at K1491-R1492 is modulated by local N-linked glycan expression within A1A2A3, and specifically inhibited by heparin binding to the A1 domain. 28279966 10 47 gly N-linked 1568:1575 arg1 local N-linked glycan expression K1491-R1492 local N-linked glycan expression K1491-R1492 Finally, we demonstrate that VWF susceptibility to plasmin proteolysis at K1491-R1492 is modulated by local N-linked glycan expression within A1A2A3, and specifically inhibited by heparin binding to the A1 domain. 28959962 4 73 gly glycopeptides 559:571 arg2 intact glycopeptides intact glycopeptides glycopeptides Here we develop a novel quantitative approach to identify intact glycopeptides from comparative proteomic data sets, allowing us not only to infer complex glycan structures but also to directly map them to sites within the associated proteins at the proteome scale. 29046357 4 17 part_of B 668:668 arg1 the sTn-linked glycopeptide glycoprotein B the sTn-linked glycopeptide Cterm Site glycoprotein B glycopeptide We previously determined the crystal structure of PILRα complexed with the sTn-linked glycopeptide of glycoprotein B, revealing the simultaneous recognition of sTn and peptide by the receptor. 29046357 4 20 part_of sTn-linked 628:637 arg1 the sTn-linked glycopeptide sTn the sTn-linked glycopeptide OGER Site sTn Q05639 glycopeptide We previously determined the crystal structure of PILRα complexed with the sTn-linked glycopeptide of glycoprotein B, revealing the simultaneous recognition of sTn and peptide by the receptor. 28276098 1 9 gly glycoprotein 209:220 arg1 glycoprotein properties glycoprotein properties Fterm glycoprotein Elucidating the effects of oligosaccharides on glycoprotein properties, such as local conformational changes, stability, and dynamics, has still been challenging. 28371609 5 91 gly Asn297-linked 1114:1126 arg1 PPB-WT and TPB-WT Asn297-linked glycans Asn297 PPB-WT and TPB-WT Asn297-linked glycans Asn297 Peptide analysis followed by the identification of glycomodified peptides using MALDI-TOF/TOF showed that PPB-WT and TPB-WT Asn297-linked glycans are mainly of complex type GnGnXF. 25253131 3 42 gly glycoproteins 885:897 arg1 immunopurified glycoproteins immunopurified glycoproteins Fterm glycoproteins Glutathione S-transferase (GST) fused to the amino-terminus of galectin cDNAs has proven to be especially useful for preparation of recombinant galectins in bacteria for use on glycan arrays, in experiments with cultured or isolated cells, and in pull-down assays with immunopurified glycoproteins. 28218815 2 5 gly modified 345:352 arg1 these enzymes AND sialylated N-glycans these enzymes sialylated N-glycans Fterm enzymes In silico analysis of carboxylesterases CES1 and CES2 suggested that these enzymes are modified with sialylated N-glycans, which are proposed to stabilize the active multimeric forms of these enzymes. 28218815 2 21 gly sialylated 359:368 arg1 sialylated N-glycans sialylated N-glycans In silico analysis of carboxylesterases CES1 and CES2 suggested that these enzymes are modified with sialylated N-glycans, which are proposed to stabilize the active multimeric forms of these enzymes. 25422905 2 35 gly residues 461:468 arg1 amino acid residues Asn28 and Asn63 residues Asn28 and Asn63 residues Asn28 and Asn63 In females and males, Gd is found in four distinct glycoforms-A, C, F and S-that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. 25422905 2 35 gly residues 461:468 arg1 Asn63 residues Asn28 and Asn63 residues Asn28 and Asn63 In females and males, Gd is found in four distinct glycoforms-A, C, F and S-that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. 25422905 2 54 gly residues 461:468 arg1 different N-linked oligosaccharide side chains residues Asn28 and Asn63 different N-linked oligosaccharide side chains residues Asn28 and Asn63 In females and males, Gd is found in four distinct glycoforms-A, C, F and S-that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. 25422905 2 35 gly residues 461:468 arg1 Asn63 residues Asn28 and Asn63 residues Asn28 and Asn63 In females and males, Gd is found in four distinct glycoforms-A, C, F and S-that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. 25422905 2 54 gly residues 461:468 arg1 different N-linked oligosaccharide side chains residues Asn28 and Asn63 different N-linked oligosaccharide side chains residues Asn28 and Asn63 In females and males, Gd is found in four distinct glycoforms-A, C, F and S-that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. 25422905 2 54 gly residues 461:468 arg1 different N-linked oligosaccharide side chains residues Asn28 and Asn63 different N-linked oligosaccharide side chains residues Asn28 and Asn63 In females and males, Gd is found in four distinct glycoforms-A, C, F and S-that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. 28076415 1 88 part_of regions 158:164 arg1 HIV-1 envelope trimers trimers regions Fterm trimers 155971 A comprehensive understanding of the regions on HIV-1 envelope trimers targeted by broadly neutralizing antibodies may contribute to rational design of an HIV-1 vaccine. 27716795 10 47 gly glycopeptide 2004:2015 arg2 glycopeptide immunogens glycopeptide immunogens glycopeptide These studies suggest that glycopeptide immunogens can be designed to stabilize the most relevant Env conformations to focus the immune response on key neutralizing epitopes. 27112851 6 14 part_of PLA/NCC 1118:1124 arg1 PLA/NCC composites NCC PLA/NCC composites OGER Site NCC P55017 composites The phase interface adhesion also plays an important role in the mechanical behavior of PLA/NCC composites, which is further revealed by the nanomechanical analysis using atom force microscopy. 24796651 1 25 gly glycopeptides 405:417 arg2 intact glycopeptides intact glycopeptides glycopeptides Data dependent acquisition (DDA) of higher collision energy dissociation (HCD)-MS(2) followed by electron transfer dissociation (ETD)-MS(2) upon detection of glycan-specific oxonium is one of the better approaches in current LC-MS(2) analysis of intact glycopeptides. 28955895 3 68 part_of glycoproteins 495:507 arg1 certain sites glycoproteins certain sites Fterm Site glycoproteins sites Glycosylation at certain sites of different genotypes HCV glycoproteins shows substantial differences in functions of the individual glycans (Goffard et al., 2005; Helle et al., 2010) [1], [2]. 28990779 3 15 gly fucosylation 648:659 arg1 intact N-glycoproteins intact N-glycoproteins Fterm N-glycoproteins We report in this paper the design and generation of potential α1,6-fucosynthase and fucoligase for direct core fucosylation of intact N-glycoproteins. 28990779 3 68 gly N-glycoproteins 671:685 arg1 direct core fucosylation N-glycoproteins direct core fucosylation Fterm N-glycoproteins We report in this paper the design and generation of potential α1,6-fucosynthase and fucoligase for direct core fucosylation of intact N-glycoproteins. 25569238 4 33 part_of preproteins 421:431 arg1 type IV pilin-like signal peptides proteins type IV pilin-like signal peptides Fterm Site proteins peptides Both pilins and archaellins are made initially as preproteins with type IV pilin-like signal peptides, which must be removed by a prepilin peptidase-like enzyme. 28955895 7 3 gly glycoproteins 1330:1342 arg1 misfolded glycoproteins misfolded glycoproteins Fterm glycoproteins Our data showed that, in contrast to HCV 1a and 2a, the folding of HCV 1b envelope proteins E2 (sites N1, N2, N10) and E1 (sites N1, N5) was disrupted, however that did not prevent the formation of virus-like particles (VLP) with misfolded glycoproteins having densities typical for HCV particles containing RNA fragments. 27313224 4 32 part_of PNG 797:799 arg1 PNG sites PNG sites OGER Site PNG Q96C90 sites SV2C-LD4 exhibits the strongest protein-protein interaction and comprises five putative N-glycosylation sites (PNG sites). 29066631 10 95 gly disialylated 2207:2218 arg1 fucosylated and disialylated type 1 and 2 chains fucosylated and disialylated type 1 and 2 chains The complexity of their terminal glycotopes including variants of fucosylated and disialylated type 1 and 2 chains would otherwise not be adequately delineated by any conventional LC-MS/MS analysis. 29066631 10 99 gly fucosylated 2191:2201 arg1 fucosylated and disialylated type 1 and 2 chains fucosylated and disialylated type 1 and 2 chains The complexity of their terminal glycotopes including variants of fucosylated and disialylated type 1 and 2 chains would otherwise not be adequately delineated by any conventional LC-MS/MS analysis. 24334764 3 0 gly glycosylation 613:625 arg1 CS serum proteins CS serum proteins Fterm proteins The glycosylation status of CS serum proteins showed a very unusual pattern of glycosylation characterized by a significant accumulation of agalactosylated fucosylated structures as well as asialylated fucosylated structures demonstrating a major defect of glycan maturation in CS. 24334764 3 14 gly asialylated 799:809 arg1 asialylated fucosylated structures asialylated fucosylated structures The glycosylation status of CS serum proteins showed a very unusual pattern of glycosylation characterized by a significant accumulation of agalactosylated fucosylated structures as well as asialylated fucosylated structures demonstrating a major defect of glycan maturation in CS. 24334764 3 66 gly fucosylated 811:821 arg1 asialylated fucosylated structures asialylated fucosylated structures The glycosylation status of CS serum proteins showed a very unusual pattern of glycosylation characterized by a significant accumulation of agalactosylated fucosylated structures as well as asialylated fucosylated structures demonstrating a major defect of glycan maturation in CS. 24334764 3 76 gly fucosylated 765:775 arg1 agalactosylated fucosylated structures agalactosylated fucosylated structures The glycosylation status of CS serum proteins showed a very unusual pattern of glycosylation characterized by a significant accumulation of agalactosylated fucosylated structures as well as asialylated fucosylated structures demonstrating a major defect of glycan maturation in CS. 26147064 8 65 part_of heparin 1496:1502 arg1 heparin product composition heparin heparin product composition Fterm Site heparin position Use of highly sensitive liquid chromatography-mass spectrometry and nuclear magnetic resonance analytical techniques reveal a minimum impact of chitosan-based purification on heparin product composition. 25964011 5 11 gly N-glycopeptides 811:825 arg2 both intact and deglycosylated N-glycopeptides both intact and deglycosylated N-glycopeptides N-glycopeptides Our analytical approach comprised ZIC-HILIC enrichment of N-glycopeptides from HEL trypsin digest, deglycosylation by (18)O/PNGase F as well as by various endoglycosidases, and LC-MS/MS analysis of both intact and deglycosylated N-glycopeptides engaging multiple techniques of ionization and fragmentation. 25964011 5 31 gly N-glycopeptides 640:654 arg2 N-glycopeptides N-glycopeptides N-glycopeptides Our analytical approach comprised ZIC-HILIC enrichment of N-glycopeptides from HEL trypsin digest, deglycosylation by (18)O/PNGase F as well as by various endoglycosidases, and LC-MS/MS analysis of both intact and deglycosylated N-glycopeptides engaging multiple techniques of ionization and fragmentation. 25964011 5 41 gly deglycosylation 681:695 arg1 both intact and deglycosylated N-glycopeptides both intact and deglycosylated N-glycopeptides N-glycopeptides Our analytical approach comprised ZIC-HILIC enrichment of N-glycopeptides from HEL trypsin digest, deglycosylation by (18)O/PNGase F as well as by various endoglycosidases, and LC-MS/MS analysis of both intact and deglycosylated N-glycopeptides engaging multiple techniques of ionization and fragmentation. 25964011 5 57 gly deglycosylated 796:809 arg1 both intact and deglycosylated N-glycopeptides both intact and deglycosylated N-glycopeptides N-glycopeptides Our analytical approach comprised ZIC-HILIC enrichment of N-glycopeptides from HEL trypsin digest, deglycosylation by (18)O/PNGase F as well as by various endoglycosidases, and LC-MS/MS analysis of both intact and deglycosylated N-glycopeptides engaging multiple techniques of ionization and fragmentation. 25422905 1 15 gly glycoprotein 179:190 arg1 an abundant glycoprotein an abundant glycoprotein Fterm glycoprotein Human glycodelin (Gd) is an abundant glycoprotein from the lipocalin family and is involved in crucial biological processes such as reproduction and immune reaction. 27302155 6 25 gly N-glycopeptides 947:961 arg2 these N-glycopeptides these N-glycopeptides N-glycopeptides Simultaneously with these N-glycopeptides, 53 compositional glycoforms of the hinge region O-glycopeptide were profiled in a single high resolution MALDI-FTICR spectrum. 27302155 6 30 gly glycoforms 981:990 arg1 the hinge region O-glycopeptide the hinge region O-glycopeptide O-glycopeptide Simultaneously with these N-glycopeptides, 53 compositional glycoforms of the hinge region O-glycopeptide were profiled in a single high resolution MALDI-FTICR spectrum. 27302155 6 71 gly O-glycopeptide 1012:1025 arg2 the hinge region O-glycopeptide the hinge region O-glycopeptide O-glycopeptide Simultaneously with these N-glycopeptides, 53 compositional glycoforms of the hinge region O-glycopeptide were profiled in a single high resolution MALDI-FTICR spectrum.