doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_base_np	arg_protein	arg_domain	arg_site	arg_sugar	PSource	SiteSource	NProtein	NID	SiteName	sent_text
33792699	0	9	gly	Bisecting-GlcNAc	0:15	arg1	Asn388			Asn388	Asn388		AminoAcid			Asn388	Bisecting-GlcNAc on Asn388 is characteristic to ERC/mesothelin expressed on epithelioid mesothelioma cells.
34494876	7	107	gly	N-glycosylation	996:1010	arg1	ACE2	ACE2				PUBTATOR		ACE2	Q9BYF1		The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.
35662980	3	60	gly	enzyme	468:473	arg1	an Asn-linked oligosaccharide	FUT8 enzyme			an Asn-linked oligosaccharide	PUBTATOR		FUT8 enzyme	2530		Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.
32929138	4	69	gly	glycoprotein	738:749	arg1	the S glycoprotein	the S glycoprotein				Cterm		S glycoprotein	43740568		Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.
32426967	8	76	gly	glycopeptide	1577:1588	arg1	A1AT	A1AT				PUBTATOR		A1AT	5265		We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.
32454127	5	78	gly	tri-glycosylated	727:742	arg1	tri-glycosylated PrP	tri-glycosylated PrP				PUBTATOR		PrP	19122		Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.
34151424	0	8	gly	deglycosylation	11:25	arg1	the egg ovomucoid	the egg ovomucoid				PUBTATOR		ovomucoid	416236		Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.
34127537	5	36	gly	N-glycosylation	710:724	arg1	IgAN	IgAN				PUBTATOR		IgAN	60498		To date, limited detail is known about IgA O- and N-glycosylation in IgAN.
34328324	2	6	gly	found	469:473	arg2	S-RBD AND the new O-glycans	S-RBD			the new O-glycans	PUBTATOR		S	43740568		However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.
32059888	5	38	gly	spp	997:999	arg1	polysaccharides	spp			polysaccharides	OGER		spp	Q8TCT9		This study provided a systematic profiling of polysaccharides of Polygonatum spp.
32568312	2	62	gly	glycosylation	471:483	arg1	IgGs	IgGs				Cterm		IgG			Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.
34494876	11	58	gly	deglycosylated	1854:1867	arg1	the deglycosylated ACE2 receptor	the deglycosylated ACE2 receptor				PUBTATOR		ACE2 receptor	Q9BYF1		Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.
34009123	0	32	gly	microbiota	28:37	arg1	Glycan-based shaping	a			Glycan-based shaping	PUBTATOR		a	11820		Glycan-based shaping of the microbiota during primate evolution.
34127537	0	99	gly	N-Glycosylation	7:21	arg1	Serum Immunoglobulin A	Serum Immunoglobulin A				Cterm		Serum Immunoglobulin A			O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.
33124956	0	44	gly	IL-1RI	39:44	arg1	Glycan-mediated functional assembly	IL-1RI			Glycan-mediated functional assembly	PUBTATOR		IL-1RI	3554		Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.
33734311	5	61	gly	ovalbumin	884:892	arg1	highly branched N-glycans	ovalbumin			highly branched N-glycans	PUBTATOR		ovalbumin	396058		We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.
31949166	2	35	gly	α-DG	226:229	arg1	the O-mannosyl glycan	of α-DG			the O-mannosyl glycan	PUBTATOR		of α-DG	6688		Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.
34957619	11	60	gly	α2,6-sialylation	1490:1505	arg1	E-cadherin	E-cadherin				OGER		E-cadherin	P12830		Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.
34957619	11	72	gly	E-cadherin	1510:1519	arg1	ST6GAL1-mediated α2,6-sialylation	E-cadherin			ST6GAL1-mediated α2,6-sialylation	OGER		E-cadherin	P12830		Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.
33423189	4	54	gly	glycoforms	757:766	arg1	mouse IgG2b-Fc glycoforms	mouse IgG2b-Fc glycoforms				PUBTATOR		IgG2b	16016		Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.
34822830	5	52	gly	possessed	951:959	arg1	ERP-W AND neutral sugar content	ERP-W			neutral sugar content	OGER		ERP	Q9BY08		ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.
34232070	12	28	gly	afucosylated	1976:1987	arg1	afucosylated PGT121 MAbs	afucosylated PGT121 MAbs				OGER		PGT121 MAbs			Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.
34786539	6	70	gly	site	911:914	arg1	trans&cis-infection	cis			site	OGER		cis	Q9NSE2		Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.
34019795	2	11	gly	glycosylated	256:267	arg1	Env	Env				PUBTATOR		Env	100616444		We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.
34290702	4	7	gly	found	628:632	arg2	IgG-Fc AND di-galactosylated glycan structures	IgG-Fc			di-galactosylated glycan structures	Cterm		IgG			As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.
32321762	4	69	gly	PrPC	746:749	arg1	GPI glycan structures	PrPC			GPI glycan structures	PUBTATOR		PrPC	5621		Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.
34583196	3	42	gly	CDP-Gro	602:608	arg1	TagD	CDP			TagD	PUBTATOR		CDP	4878		Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.
34285147	0	48	gly	N-glycosylation	0:14	arg1	immunoglobulin G	immunoglobulin G				Cterm		immunoglobulin G			N-glycosylation of immunoglobulin G predicts incident hypertension.
32485649	2	13	gly	glycopeptides	367:379	arg1	hemopexin and haptoglobin proteins	hemopexin and haptoglobin proteins				PUBTATOR		haptoglobin proteins	3240		In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.
33657316	2	73	gly	glycosylated	529:540	arg1	the heavily glycosylated S protein trimer	the heavily glycosylated S protein trimer				PUBTATOR		S protein trimer	7448		CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.
32147470	3	4	gly	IgG-	490:493	arg1	different polysaccharide-based biogels	IgG			different polysaccharide-based biogels	Cterm		IgG			To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.
33063473	3	30	gly	N-glycosylation	320:334	arg1	PD-1	PD-1				OGER		PD-1	P18621		However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.
32162920	0	46	gly	Transferrin	43:53	arg1	the Glycan Profile	Mouse Transferrin			the Glycan Profile	PUBTATOR		Mouse Transferrin	22041		Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.
34339169	8	28	gly	deglycosylated	1483:1496	arg1	fibronectin	fibronectin				PUBTATOR		fibronectin	2335		We also demonstrated that the recognition of human plasma fibronectin by Ata requires this ability to bind glycans, as the interaction between Ata and fibronectin does not occur when fibronectin is deglycosylated.
33709491	3	41	gly	glycoforms	445:454	arg1	RBD glycoforms	RBD glycoforms				Cterm		RBD			However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.
32743578	1	7	gly	glycosylated	187:198	arg1	its highly glycosylated trimeric Spike protein	its highly glycosylated trimeric Spike protein				PUBTATOR		Spike protein	43740568		The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.
32743578	1	27	gly	glycoprotein	265:276	arg1	the cell surface receptor ACE2 glycoprotein	the cell surface receptor ACE2 glycoprotein				PUBTATOR		ACE2 glycoprotein	59272		The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.
34288669	5	74	gly	glycoforms	1163:1172	arg1	hCG glycoforms	hCG glycoforms				PUBTATOR		hCG	1511		Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.
32102878	5	28	gly	residues	839:846	arg1	Env	Env			residues	PUBTATOR		Env	30816		The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.
33758835	3	11	gly	glycosylation	389:401	arg1	recombinant SARS-CoV-2 spike proteins	recombinant SARS-CoV-2 spike proteins				PUBTATOR		spike proteins	43740568		Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.
32205858	0	40	gly	glycosylation	8:20	arg1	glycodelin	glycodelin				PUBTATOR		glycodelin in	5047		Altered glycosylation of glycodelin in endometrial carcinoma.
32045434	2	74	gly	Glycosylation	214:226	arg1	hCG	hCG				PUBTATOR		hCG	1511		Glycosylation of hCG is known to be essential for its biological activity.
32501643	11	21	gly	glycosylated	1589:1600	arg1	glycosylated APOE	glycosylated APOE				PUBTATOR		APOE	348		Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.
32162920	7	50	gly	CIA	1299:1301	arg1	glycan-based biomarkers	CIA			glycan-based biomarkers	OGER		CIA	Q9CQE6		Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.
31907284	2	55	gly	heterogeneity	552:564	arg1	the final IgG-Fc glycosylation profile	the final IgG-Fc glycosylation profile				PUBTATOR		e	51428		The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.
35049867	6	35	gly	GLP	946:948	arg1	The total sugar content	GLP			The total sugar content	OGER		GLP	Q9H9B1		The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.
31967297	4	84	part_of	CD16a	782:786	arg1	position 48	CD16a		position 48		PUBTATOR	SpecificSite	CD16a	2214	leucine at position 48	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.
32505675	5	10	gly	NOTCH1	715:720	arg1	EGF repeat 27	NOTCH1			EGF repeat 27	PUBTATOR		NOTCH1	P46531		EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.
32800953	3	56	gly	Se-SPP	691:696	arg1	carbohydrate	Se-SPP			carbohydrate	OGER		Se-SPP	Q8TCT9		The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).
32800953	3	56	gly	Se-SPP	691:696	arg1	the monosaccharide composition	Se-SPP			the monosaccharide composition	OGER		Se-SPP	Q8TCT9		The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).
35178379	0	55	gly	SARS-CoV-2	57:66	arg1	Human Blood Group A Glycan Structures	From SARS			Human Blood Group A Glycan Structures	OGER		From SARS	P49591		In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.
33064451	7	66	gly	proteins	1286:1293	arg1	N-glycan profiles	spike proteins			N-glycan profiles	PUBTATOR		spike proteins	43740568		In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.
32623356	0	99	gly	N-glycosylation	0:14	arg1	HMGB1	HMGB1				PUBTATOR		HMGB1	3146		N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.
32623356	0	99	gly	N-glycosylation	0:14	arg1	High Mobility Group Box 1 protein	High Mobility Group Box 1 protein				PUBTATOR		High Mobility Group Box 1 protein	3146		N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.
34067878	6	3	gly	RBD-ACE2	839:846	arg1	oligomannose MAN9 glycans	RBD-ACE2			oligomannose MAN9 glycans	PUBTATOR		RBD-ACE2	Q9BYF1		To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.
34067878	6	3	gly	RBD-ACE2	839:846	arg1	complex FA2 glycans	RBD-ACE2			complex FA2 glycans	PUBTATOR		RBD-ACE2	Q9BYF1		To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.
34067878	6	88	gly	RBD-ACE2	780:787	arg1	oligomannose MAN9 glycans	RBD-ACE2			oligomannose MAN9 glycans	PUBTATOR		RBD-ACE2	Q9BYF1		To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.
34067878	6	88	gly	RBD-ACE2	780:787	arg1	complex FA2 glycans	RBD-ACE2			complex FA2 glycans	PUBTATOR		RBD-ACE2	Q9BYF1		To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.
34067878	6	8	gly	glycans	766:772	arg1	ACE2	ACE2			glycans	PUBTATOR		ACE2	Q9BYF1		To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.
32577644	2	83	gly	glycosylated	322:333	arg1	the extensively glycosylated SARS-CoV-2 spike (S) protein	the extensively glycosylated SARS-CoV-2 spike (S) protein				OGER		S) protein	Q15517		Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).
32438810	5	46	gly	glycoprotein	852:863	arg1	GlyCAM-1	GlyCAM-1				PUBTATOR		GlyCAM-1	282430		GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.
32438810	5	58	gly	carrying	865:872	arg1	GlyCAM-1 AND tri- and tetra-antennary structures	GlyCAM-1			tri- and tetra-antennary structures	PUBTATOR		GlyCAM-1	282430		GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.
34494876	3	62	gly	presence	448:455	arg2	ACE2 AND N-linked glycans	ACE2			N-linked glycans	PUBTATOR		ACE2	Q9BYF1		Previous work has demonstrated the presence of N-linked glycans in ACE2.
32205858	6	28	gly	isoform	1028:1034	arg1	previously reported glycan structures	glycodelin isoform			previously reported glycan structures	PUBTATOR		glycodelin isoform	5047		However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.
34925422	5	25	gly	N-glycosylation	728:742	arg1	the protein N-glycosylation pathway	the protein N-glycosylation pathway				Cterm		N-glycosylation			Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.
34611825	9	69	part_of	Recombinant	1183:1193	arg1	His6-RbL	Recombinant		His6-RbL		Cterm	AminoAcid	Recombinant		His6	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.
33141553	0	95	gly	Glycosylation	0:12	arg1	Serum Clusterin	Serum Clusterin				PUBTATOR		Clusterin	1191		Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.
33063473	4	10	gly	glycosylated	733:744	arg1	glycosylated PD-1	glycosylated PD-1				OGER		PD-1	P18621		Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.
33063473	4	33	gly	non-glycosylated	648:663	arg1	non-glycosylated PD-1 proteins	non-glycosylated PD-1 proteins				OGER		PD-1 proteins	P18621		Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.
33063473	4	43	gly	composition	533:543	arg1	PD-1	PD-1			composition	OGER		PD-1	P18621		Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.
33077685	2	0	gly	N-glycosylation	298:312	arg1	S protein	S protein				PUBTATOR		S protein	Q15517		Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.
33675758	0	41	gly	deglycosylated	22:35	arg1	deglycosylated human IgG1	deglycosylated human IgG1				OGER		IgG1	P01857		Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.
33792699	7	33	part_of	ERC/mesothelin	1413:1426	arg1	Asn388	ERC/mesothelin		Asn388		PUBTATOR	AminoAcid	ERC/mesothelin	10232	Asn388	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.
34611825	0	66	gly	Bean	172:175	arg1	N-Acetyl-D-Glucosamine	Rice Bean			N-Acetyl-D-Glucosamine	OGER		Rice Bean	Q3B7T3		Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).
34611825	0	66	gly	Bean	172:175	arg1	β-D-Galactose	Rice Bean			β-D-Galactose	OGER		Rice Bean	Q3B7T3		Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).
33118822	6	12	part_of	Fc	1307:1308	arg1	Fc fragment	Fc		Fc fragment		Cterm	SpecificSite	Fc		fragment Asn 61	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.
33205457	11	63	gly	ChN-F	2056:2060	arg1	the oppositely charged polysaccharide particles	ChN			the oppositely charged polysaccharide particles	OGER		ChN	P15882		CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.
33675758	13	58	gly	deglycosylated	1875:1888	arg1	deglycosylated IgG1	deglycosylated IgG1				OGER		IgG1	P01857		The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.
33675758	13	87	gly	glycosylated	1760:1771	arg1	glycosylated IgG1	glycosylated IgG1				OGER		IgG1	P01857		The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.
34523784	3	39	gly	carries	354:360	arg1	EPO AND three N-glycans	EPO			three N-glycans	PUBTATOR		EPO	2056		EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge.
32423029	1	57	gly	glycosylation	191:203	arg1	Notch signaling	Notch signaling				PUBTATOR		Notch	31293		Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.
33954318	9	23	gly	fucosylated	1471:1481	arg1	other fucosylated hMOs	other fucosylated hMOs				PUBTATOR		hMOs	4342		We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.
32973324	7	18	gly	deglycosylation	998:1012	arg1	SIgA	SIgA				Cterm		IgA	973		In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.
34355537	7	74	gly	sialylated	1159:1168	arg1	sialylated and asialo mAb1	sialylated and asialo mAb1				Cterm		mAb1	P01857		The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.
34355537	5	26	gly	IgG1	846:849	arg1	sialic acid-enriched species	IgG1			sialic acid-enriched species	OGER		IgG1	P01857		In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.
32477333	3	46	gly	derived	494:500	arg2	bovine lactoferrin AND N-glycans	bovine lactoferrin			N-glycans	PUBTATOR		lactoferrin	280846		In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.
32477333	3	46	gly	derived	494:500	arg1	bLF AND N-glycans	bLF			N-glycans	Cterm		bLF	280846		In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.
32205858	3	77	gly	glycosylation	426:438	arg1	glycodelin	glycodelin				PUBTATOR		glycodelin	5047		Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.
32477333	0	96	gly	Lactoferrin	52:62	arg1	Dietary N-Glycans	Lactoferrin			Dietary N-Glycans	PUBTATOR		Lactoferrin	280846		Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.
32568201	7	78	gly	observed	1156:1163	arg2	CSF AND two glycans	CSF			two glycans	OGER		CSF			For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.
34687022	1	33	gly	glycoprotein	143:154	arg1	Immunoglobulin G	Immunoglobulin G				Cterm		Immunoglobulin G			Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.
32426967	2	30	gly	glycoforms	398:407	arg1	A1AT	A1AT				PUBTATOR		A1AT	5265		In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.
32426967	2	30	gly	glycoforms	398:407	arg1	serum α-1-antitrypsin	serum α-1-antitrypsin				PUBTATOR		-1-antitrypsin	5265		In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.
33650863	2	71	gly	UMOD	245:248	arg1	Comprehensive glycan profiling	UMOD			Comprehensive glycan profiling	PUBTATOR		UMOD	7369		Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.
32066783	3	44	gly	glycoforms	595:604	arg1	PSA	PSA				PUBTATOR		PSA	354		In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.
32066783	3	50	gly	PSA	396:398	arg1	the glycans	PSA			the glycans	PUBTATOR		PSA	354		In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.
34925381	5	54	gly	glycan-masking	1193:1206	arg1	Ad-S-R158N/Y160T	S			glycan-masking	PUBTATOR		S	Q15517		The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.
34494876	1	34	gly	glycoprotein	211:222	arg1	Human angiotensin I-converting enzyme 2	Human angiotensin I-converting enzyme 2				PUBTATOR		Human angiotensin I-converting enzyme 2	59272		Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.
34582712	7	44	gly	glycosylation	1319:1331	arg1	α-DG	α-DG				Cterm		DG	Q14118		The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.
32066783	5	22	gly	sialylated	830:839	arg1	PSA	PSA				PUBTATOR		PSA	354		PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.
34019795	3	54	gly	patch	383:387	arg1	Env	Env			patch	PUBTATOR		Env	100616444		2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.
33675758	4	79	gly	glycosylated	481:492	arg1	monoclonal human IgG1	monoclonal human IgG1				OGER		IgG1	P01857		Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.
33675758	4	83	gly	deglycosylated	498:511	arg1	monoclonal human IgG1	monoclonal human IgG1				OGER		IgG1	P01857		Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.
32477333	13	55	gly	bLF	2203:2205	arg1	isolated N-glycans	bLF			isolated N-glycans	Cterm		bLF	280846		Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.
33245474	12	18	gly	glycoprotein	1646:1657	arg1	SARS-CoV-2 S glycoprotein stimulation	SARS-CoV-2 S glycoprotein stimulation				PUBTATOR		S glycoprotein	43740568		Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.
33484712	3	20	gly	attached	638:645	arg1	the S-protein AND glycans	the S-protein			glycans	PUBTATOR		S-protein	Q15517		Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.
32321762	8	94	gly	PrPC	1593:1596	arg1	the GPI glycan structure	PrPC			the GPI glycan structure	PUBTATOR		PrPC	5621		In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.
32502344	7	27	gly	MUC1	1100:1103	arg1	Gal/GalNAc	MUC1			Gal/GalNAc	PUBTATOR		MUC1	4582		Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.
32502344	7	27	gly	MUC1	1100:1103	arg1	the terminal galactose/N-acetylgalactosamine	MUC1			the terminal galactose/N-acetylgalactosamine	PUBTATOR		MUC1	4582		Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.
32205858	5	66	gly	Gd	724:725	arg1	Glycans	HEC-1B Gd			Glycans	PUBTATOR		HEC-1B Gd	5047		Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.
32005975	7	5	gly	O-glycans	1076:1084	arg1	EPHA2	EPHA2			O-glycans	PUBTATOR		EPHA2	1969		O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.
32871472	5	5	gly	N-glycosylation	571:585	arg1	PD-L1	PD-L1				PUBTATOR		PD-L1	29126		Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.
32767150	8	36	gly	O-glycosylation	957:971	arg1	CGB7	CGB7				PUBTATOR		CGB7	94027		We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.
32767150	8	36	gly	O-glycosylation	957:971	arg1	CGB3/5/8	CGB3/5/8				PUBTATOR		CGB3	1082		We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.
34757822	6	16	gly	fucosylated	975:985	arg1	fucosylated HMOs	fucosylated HMOs				OGER		HMOs	P00540		One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.
34113353	1	1	gly	attached	111:118	arg1	IgG AND Glycans	IgG			Glycans	PUBTATOR		IgG	668542		Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.
34113353	1	1	gly	attached	111:118	arg1	immunoglobulin G AND Glycans	immunoglobulin G			Glycans	Cterm		immunoglobulin G			Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.
33484712	4	57	gly	glycosylated	862:873	arg1	a fully glycosylated S-protein	a fully glycosylated S-protein				PUBTATOR		S-protein	Q15517		Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.
34328324	5	77	gly	glycoprotein	1509:1520	arg1	the S glycoprotein	the S glycoprotein				PUBTATOR		S glycoprotein	43740568		These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.
35662980	9	43	gly	glycosylation	1554:1566	arg1	KO mgat1	KO mgat1				OGER		KO mgat1	Q91ZV4		Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.
33689337	3	77	gly	protein	754:760	arg1	glycan functions	S protein			glycan functions	PUBTATOR		S protein	Q15517		In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.
32280962	3	50	gly	PSG1	528:531	arg1	the glycan composition	PSG1			the glycan composition	PUBTATOR		PSG1	5669		We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.
34757822	4	21	gly	fucosylated	715:725	arg1	fucosylated HMOs	fucosylated HMOs				OGER		HMOs	P00540		During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.
34890647	2	25	gly	IgG1	451:454	arg1	glycans	IgG1			glycans	OGER		IgG1	P01857		Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.
33065977	3	25	gly	N-glycosylation	414:428	arg1	IgG	IgG				Cterm		IgG			We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.
32577644	8	44	gly	protein	1510:1516	arg1	the glycan shield	S protein			the glycan shield	OGER		S protein	Q15517		Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.
33064451	3	74	gly	N-glycosylation	527:541	arg1	the SARS-CoV-2 spike proteins	the SARS-CoV-2 spike proteins				PUBTATOR		spike proteins	43740568		We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.
32426967	4	8	gly	glycoforms	922:931	arg1	A1AT	A1AT				PUBTATOR		A1AT	5265		Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.
34127537	6	10	gly	glycopeptides	916:928	arg1	serum IgA	serum IgA				PUBTATOR		IgA	973		METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.
34127537	6	84	gly	N-glycosylation	784:798	arg1	IgA2	IgA2				PUBTATOR		IgA2	973		METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.
34127537	6	84	gly	N-glycosylation	784:798	arg1	serum IgA1	serum IgA1				PUBTATOR		IgA1	P01876		METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.
34067878	10	85	gly	glycan	1460:1465	arg1	Asn90			Asn90	Asn90		AminoAcid			Asn90	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.
32885971	2	50	gly	glycosylated	292:303	arg1	S	S				PUBTATOR		S	43740568		As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.
33631789	6	66	gly	[2	1177:1178	arg1	fucose	acid; [2			fucose	OGER		acid; [2	Q86YD1		Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.
32896318	7	70	gly	glycoprotein	1253:1264	arg1	the hydroxyproline-rich glycoprotein (HRGP) family	the hydroxyproline-rich glycoprotein (HRGP) family				PUBTATOR		hydroxyproline-rich glycoprotein	3273		We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.
32205858	1	26	gly	glycoprotein	84:95	arg1	Glycodelin	Glycodelin				PUBTATOR		Glycodelin	5047		Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.
32841605	1	26	gly	glycosylated	133:144	arg1	its highly glycosylated trimeric Spike protein	its highly glycosylated trimeric Spike protein				PUBTATOR		Spike protein	43740568		The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.
32059888	0	28	gly	spp	88:90	arg1	polysaccharides	spp			polysaccharides	OGER		spp	Q8TCT9		Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.
32059888	0	28	gly	spp	88:90	arg1	saccharide mapping	spp			saccharide mapping	OGER		spp	Q8TCT9		Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.
32059888	0	49	gly	mapping	32:38	arg1	four common Polygonatum spp	spp			mapping	OGER		spp	Q8TCT9		Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.
32339582	4	68	gly	α-amylase	731:739	arg1	The glucose adsorption capacity	-amylase			The glucose adsorption capacity	PUBTATOR		-amylase	100792499		The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.
33756033	7	21	gly	non-glycosylated	946:961	arg1	non-glycosylated IL-6	non-glycosylated IL-6				PUBTATOR		IL-6	24498		Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.
33756033	7	48	gly	glycoforms	997:1006	arg1	IL-6 glycoforms	IL-6 glycoforms				PUBTATOR		IL-6	24498		Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.
32045434	5	62	gly	hyperglycosylated	803:819	arg1	hyperglycosylated hCG	hyperglycosylated hCG				OGER		hCG			In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.
32568201	2	32	gly	glycosylated	340:351	arg1	CSF	CSF				OGER		CSF			In cerebrospinal fluid (CSF), apoE is heavily glycosylated.
32568201	2	32	gly	glycosylated	340:351	arg1	apoE	apoE				PUBTATOR		apoE	348		In cerebrospinal fluid (CSF), apoE is heavily glycosylated.
32066783	0	52	gly	glycosylation	9:21	arg1	prostate-specific antigen	prostate-specific antigen				PUBTATOR		prostate-specific antigen	354		Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.
33909026	2	34	gly	O-glycosylation	520:534	arg1	ER	ER				PUBTATOR		ER	2069		Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.
34687018	4	6	gly	glycosylation	764:776	arg1	antigen-specific IgG	antigen-specific IgG				Cterm		IgG			In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.
32049016	2	1	gly	carbohydrate	366:377	arg1	Env	Env			carbohydrate	PUBTATOR		Env	100616444		Until now, the dogma has been that HIV-1 lacks O-linked carbohydrate on Env.
32973324	5	33	gly	SIgA	626:629	arg1	enzymatically removed N-glycans	IgA			enzymatically removed N-glycans	Cterm		IgA	973		Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.
32973324	5	60	gly	SIgA	620:623	arg1	enzymatically removed N-glycans	IgA			enzymatically removed N-glycans	Cterm		IgA	973		Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.
32433878	1	2	gly	glycosylation	220:232	arg1	ECM	ECM				OGER		ECM	Q13201		Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.
34523784	4	10	gly	glycoforms	512:521	arg1	EPO	EPO				PUBTATOR		EPO	2056		We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.
34823411	5	49	gly	GLP	963:965	arg1	The monosaccharide composition	GLP			The monosaccharide composition	PUBTATOR		GLP	77683		The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.
34823411	5	49	gly	GLP	963:965	arg1	glucose	GLP			glucose	PUBTATOR		GLP	77683		The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.
31829411	8	73	gly	N-glycoforms	1954:1965	arg1	high potency IgG1 Fc N-glycoforms	high potency IgG1 Fc N-glycoforms				OGER		IgG1	P01857		These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.
34424752	3	47	gly	N-linked	744:751	arg1	an abundant glycan structure	N-linked			an abundant glycan structure	Cterm		N-linked			The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.
34931806	7	14	gly	mucin	1090:1094	arg1	GalNAz	mucin O-linked			GalNAz	PUBTATOR		mucin O-linked	100508689		The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.
34931806	7	29	gly	O-linked	1096:1103	arg1	GalNAz	mucin O-linked			GalNAz	PUBTATOR		mucin O-linked	100508689		The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.
32871472	15	70	gly	N-glycosylated	1724:1737	arg1	N-glycosylated and ubiquitinated PD-L1	N-glycosylated and ubiquitinated PD-L1				PUBTATOR		PD-L1	29126		These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.
32045434	18	91	gly	hCG-related	2436:2446	arg1	hCG-related bisected type N-glycans	hCG			hCG-related bisected type N-glycans	OGER		hCG			hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.
34067878	10	12	part_of	ACE2	1479:1482	arg1	Asn90	ACE2		Asn90		PUBTATOR	AminoAcid	ACE2	Q9BYF1	Asn90	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.
33933541	2	34	gly	GLP	366:368	arg1	a 1 → 4)-α-D-Glcp glycoside linkage	GLP			a 1 → 4)-α-D-Glcp glycoside linkage	OGER		GLP	Q9H9B1		Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.
34494876	15	79	gly	deglycosylation	2351:2365	arg1	ACE2	ACE2				PUBTATOR		ACE2	Q9BYF1		Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.
33142561	1	28	gly	hyaluronan	221:230	arg1	the hyaluronan carbohydrate structure	hyaluronan binding protein			the hyaluronan carbohydrate structure	PUBTATOR		hyaluronan binding protein	3026		Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.
33142561	1	30	gly	binding	232:238	arg1	the hyaluronan carbohydrate structure	hyaluronan binding protein			the hyaluronan carbohydrate structure	PUBTATOR		hyaluronan binding protein	3026		Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.
32423029	3	35	gly	glycans	573:579	arg1	NOTCH2	NOTCH2			glycans	PUBTATOR		NOTCH2	4853		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32423029	3	35	gly	glycans	573:579	arg1	NOTCH1	NOTCH1			glycans	PUBTATOR		NOTCH1	4851		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32423029	3	67	gly	NOTCH1	758:763	arg1	all 17 EGF repeats	NOTCH1			all 17 EGF repeats	PUBTATOR		NOTCH1	4851		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32423029	3	67	gly	NOTCH1	758:763	arg1	16 out of 17 EGF repeats	NOTCH1			16 out of 17 EGF repeats	PUBTATOR		NOTCH1	4851		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32423029	3	72	gly	NOTCH1	758:763	arg1	the O-Glc consensus sequence	NOTCH1			the O-Glc consensus sequence	PUBTATOR		NOTCH1	4851		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32423029	3	95	gly	NOTCH2	852:857	arg1	all 17 EGF repeats	NOTCH2			all 17 EGF repeats	PUBTATOR		NOTCH2	4853		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32423029	3	95	gly	NOTCH2	852:857	arg1	16 out of 17 EGF repeats	NOTCH2			16 out of 17 EGF repeats	PUBTATOR		NOTCH2	4853		Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.
32321762	0	31	gly	GPI	63:65	arg1	sialic acid	GPI			sialic acid	OGER		GPI	P06744		α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.
33564762	0	37	gly	O-glycosylation	14:28	arg1	SARS-CoV-2 spike protein	SARS-CoV-2 spike protein				PUBTATOR		spike protein	43740568		Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.
34523784	1	41	gly	glycoprotein	146:157	arg1	Recombinant human erythropoietin	Recombinant human erythropoietin				PUBTATOR		erythropoietin	2056		Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.
33064451	0	21	gly	Proteins	68:75	arg1	the Glycan Complement	Spike Proteins			the Glycan Complement	PUBTATOR		Spike Proteins	43740568		Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.
34375000	3	2	gly	residues	440:447	arg1	SARS-CoV-2	SARS			residues	OGER		SARS	P49591		Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.
34051603	3	33	gly	β-Galp-	635:641	arg1	monosaccharide residues	Galp			monosaccharide residues	OGER		Galp	Q9UBC7		In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.
32438810	4	43	gly	carries	745:751	arg1	IgG AND fucosylated di-antennary glycans	IgG			fucosylated di-antennary glycans	Cterm		IgG			We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.
32623356	7	26	gly	N-glycosylation	919:933	arg1	HMGB1	HMGB1				PUBTATOR		HMGB1	3146		Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).
33093110	4	9	gly	O-glycans	620:628	arg1	proximal colon-derived Muc2	Muc2			O-glycans	PUBTATOR		Muc2	Q02817		In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.
33141553	8	101	gly	content	1587:1593	arg1	serum CLU	CLU			content	PUBTATOR		CLU	1191		The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.
32213588	6	11	gly	glycosylation	1072:1084	arg1	transferrin receptor protein 1	transferrin receptor protein 1				PUBTATOR		transferrin receptor protein 1	7037		Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.
33063473	0	26	gly	N-glycosylation	0:14	arg1	PD-1	PD-1				OGER		PD-1	P18621		N-glycosylation of PD-1 promotes binding of camrelizumab.
33688648	5	81	gly	glycoprotein	1291:1302	arg1	the S glycoprotein	the S glycoprotein				PUBTATOR		S glycoprotein	43740568		These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.
33262351	2	3	gly	heterogeneity	304:316	arg1	HGSC	HGSC				OGER		HGSC	P56915		To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.
33262351	2	56	gly	glycosylation	283:295	arg1	HGSC	HGSC				OGER		HGSC	P56915		To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.
34523784	2	53	gly	EPO	242:244	arg1	carbohydrate-dependent	EPO			carbohydrate-dependent	PUBTATOR		EPO	2056		The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.
33734311	0	3	gly	α1,6-fucosyltransferase	94:116	arg1	the glycan substrate acceptability	1,6-fucosyltransferase			the glycan substrate acceptability	PUBTATOR		1,6-fucosyltransferase	2530		Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).
34767945	9	87	gly	PGD-	1560:1563	arg1	significantly increased IgG1 N-glycan signatures	PGD			significantly increased IgG1 N-glycan signatures	OGER		PGD	P52209		COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.
34127537	7	72	gly	N-glycosylation	1057:1071	arg1	IgA2	IgA2				PUBTATOR		IgA2	973		RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.
34127537	7	72	gly	N-glycosylation	1057:1071	arg1	IgA1	IgA1				PUBTATOR		IgA1	P01876		RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.
33141553	7	79	gly	CLU	1504:1506	arg1	glycan content	CLU			glycan content	PUBTATOR		CLU	1191		In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.
33245474	4	13	gly	glycoproteins	697:709	arg1	human ACE2 glycoproteins	human ACE2 glycoproteins				PUBTATOR		ACE2 glycoproteins	59272		Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.
32501643	10	43	gly	APOE	1342:1345	arg1	the negatively charged sialic acid-containing glycan residue	APOE			the negatively charged sialic acid-containing glycan residue	PUBTATOR		APOE	348		The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.
33661628	6	48	gly	glycosylated	1257:1268	arg1	FcγRIIIa	FcγRIIIa				PUBTATOR		FcγRIIIa	2214		We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).
33661628	6	48	gly	glycosylated	1257:1268	arg1	the fully glycosylated CD16a Fc γ receptor	the fully glycosylated CD16a Fc γ receptor				PUBTATOR		CD16a Fc γ receptor	2214		We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).
32066783	4	70	gly	PSA	636:638	arg1	The glycan profile	PSA			The glycan profile	PUBTATOR		PSA	354		The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.
34494876	13	5	gly	deglycosylation	2062:2076	arg1	S	S				PUBTATOR		S	43740568		In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.
34494876	13	5	gly	deglycosylation	2062:2076	arg1	ACE2	ACE2				PUBTATOR		ACE2	Q9BYF1		In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.
32321762	6	13	part_of	PrPC	1157:1160	arg1	Ser231	PrPC		Ser231		PUBTATOR	AminoAcid	PrPC	5621	Ser231	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.
32321762	6	30	part_of	Gly229	1056:1061	arg1	human PrPC	PrPC		Gly229		PUBTATOR	AminoAcid	PrPC	5621	Gly229	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.
32066783	7	40	gly	PSA	1228:1230	arg1	highly branched N-glycans	PSA			highly branched N-glycans	PUBTATOR		PSA	354		Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.
33319171	3	10	gly	glycosylated	384:395	arg1	fully glycosylated HIV-1 Env	fully glycosylated HIV-1 Env				PUBTATOR		HIV-1 Env	64006		Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.
32865981	8	76	gly	released	1808:1815	arg2	ovalbumin AND N-linked glycans	ovalbumin			N-linked glycans	PUBTATOR		ovalbumin	396058		G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.
33756033	1	12	gly	glycoforms	87:96	arg1	human interleukin 6	human interleukin 6				PUBTATOR		interleukin 6	3569		A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.
33756033	1	12	gly	glycoforms	87:96	arg1	IL-6	IL-6				PUBTATOR		IL-6	3569		A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.
34873736	6	13	gly	DLP-1	1170:1174	arg1	(1→4)-β-D-Glcp	DLP-1			(1→4)-β-D-Glcp	PUBTATOR		DLP-1	71365		Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.
34873736	6	50	gly	1→4	1114:1116	arg1	(1→4)-β-D-Glcp	and (1→4			(1→4)-β-D-Glcp	OGER		and (1→4	O75717		Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.
32871472	0	47	gly	N-glycosylation	0:14	arg1	PD-L1	PD-L1				PUBTATOR		PD-L1	29126		N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.
32321401	11	61	gly	structures	1777:1786	arg1	COPD	COPD			structures	OGER		COPD	P48444		Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.
32321401	11	72	gly	structure	1695:1703	arg1	COPD	COPD			structure	OGER		COPD	P48444		Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.
32577644	4	49	gly	glycosylated	609:620	arg1	glycosylated SARS-CoV-2 S protein	glycosylated SARS-CoV-2 S protein				OGER		S protein	Q15517		Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.
32423029	2	78	gly	containing	488:497	arg1	ECD AND 29-36 epidermal growth factor-like (EGF) repeats	ECD		large extracellular domains	29-36 epidermal growth factor-like (EGF) repeats	OGER		ECD	O95905	domains	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.
33077685	6	18	gly	contained	751:759	arg1	the insect cell-expressed S protein AND 38 N-glycans	the insect cell-expressed S protein			38 N-glycans	PUBTATOR		S protein	Q15517		In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.
32568312	3	87	gly	glycopeptides	638:650	arg1	IgGs	IgGs				Cterm		IgGs			Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.
33688648	4	24	gly	O-glycoforms	974:985	arg1	the S-RBD	the S-RBD				PUBTATOR		S	43740568		By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.
33650863	3	39	gly	glycosylation	371:383	arg1	UMOD	UMOD				PUBTATOR		UMOD	7369		To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.
34495537	5	56	gly	IgG	885:887	arg1	fucosylation	thyroglobulin IgG			fucosylation	OGER		thyroglobulin IgG	P01266		However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.
34495537	5	65	gly	fucosylation	850:861	arg1	anti-thyroglobulin IgG	anti-thyroglobulin IgG				OGER		thyroglobulin IgG	P01266		However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.
33319171	4	23	gly	Env	546:548	arg1	the first complete all-atom model	HIV-1 Env			the first complete all-atom model	PUBTATOR		HIV-1 Env	64006		This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.
32426967	6	57	gly	glycopeptides	1179:1191	arg1	A1AT	A1AT				PUBTATOR		A1AT	5265		Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.
34822830	8	37	gly	α-glucosidase	1555:1567	arg1	polysaccharides	-glucosidase			polysaccharides	PUBTATOR		-glucosidase	6476		Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.
32483376	5	53	gly	receptor	993:1000	arg1	this two-step glycan-editing approach	opioid receptor delta 1			this two-step glycan-editing approach	PUBTATOR		opioid receptor delta 1	O00548		Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.
32483376	5	54	gly	opioid	986:991	arg1	this two-step glycan-editing approach	opioid receptor delta 1			this two-step glycan-editing approach	PUBTATOR		opioid receptor delta 1	O00548		Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.
34478797	1	48	gly	polysaccharides	235:249	arg1	radix ginseng Rubra	Rubra (RGR			polysaccharides	OGER		Rubra (RGR	P47804		The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).
34925381	4	63	gly	Ad-S-D428N	1010:1019	arg1	Glycan-masking	S			Glycan-masking	PUBTATOR		S	Q15517		Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.
32475574	4	15	gly	MRP-P1	678:683	arg1	polysaccharides	MRP-P1			polysaccharides	PUBTATOR		MRP-P1	54931		The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.
34067878	4	69	gly	glycosylated	463:474	arg1	ACE2	ACE2				PUBTATOR		ACE2	Q9BYF1		Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.
34067878	4	69	gly	glycosylated	463:474	arg1	the Spike	the Spike				PUBTATOR		Spike	43740568		Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.
34876606	2	10	gly	glycosylated	383:394	arg1	The spike protein	The spike protein				PUBTATOR		spike protein	43740568		The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.
32224182	7	36	gly	AAP	1247:1249	arg1	a furan type polysaccharide	AAP			a furan type polysaccharide	OGER		AAP	P08697		The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).
33063473	1	4	gly	glycosylated	75:86	arg1	PD-1	PD-1				OGER		PD-1	P18621		PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.
32568312	4	65	gly	glycopeptides	924:936	arg1	purified IgGs	purified IgGs				Cterm		IgGs			In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.
33792699	3	23	gly	glycoprotein	419:430	arg1	ERC/mesothelin	ERC/mesothelin				PUBTATOR		ERC/mesothelin	10232		ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.
33245474	8	68	gly	N-glycans	1181:1189	arg1	its main receptor ACE2	ACE2			N-glycans	PUBTATOR		ACE2	59272		In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.
33245474	8	68	gly	N-glycans	1181:1189	arg1	SARS-CoV-2 S	S			N-glycans	PUBTATOR		S	43740568		In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.
33064451	2	80	gly	glycosylated	389:400	arg1	The spike protein	The spike protein				PUBTATOR		spike protein	43740568		The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection.
33675758	14	21	gly	deglycosylated	2029:2042	arg1	deglycosylated IgG1	deglycosylated IgG1				OGER		IgG1	P01857		These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.
34288669	4	4	gly	glycoforms	693:702	arg1	recombinant hCG	recombinant hCG				PUBTATOR		hCG	1511		In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.
32005658	5	71	gly	sialylated	1154:1163	arg1	heavily sialylated CHO-sKlotho	heavily sialylated CHO-sKlotho				Cterm		CHO-sKlotho	Q9UEF7		Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.
32005658	5	89	gly	hyposialylated	1253:1266	arg1	hyposialylated HEK-sKlotho	hyposialylated HEK-sKlotho				OGER		HEK	P29320		Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.
34285147	1	69	gly	Glycosylation	79:91	arg1	IgG	IgG				PUBTATOR		IgG	668542		OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.
34285147	1	69	gly	Glycosylation	79:91	arg1	immunoglobulin G	immunoglobulin G				Cterm		immunoglobulin G			OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.
34151424	4	31	gly	deglycosylated	639:652	arg1	deglycosylated OVM	deglycosylated OVM				PUBTATOR		OVM	416236		However, the OVM glycated by mannosan (MOS) and deglycosylated OVM exhibited higher allergenicity than native OVM.
33657316	6	25	gly	trimers	1300:1306	arg1	glycan processing	S protein trimers			glycan processing	PUBTATOR		S protein trimers	7448		The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.
33076454	4	9	gly	G	629:629	arg1	enzymatically released N-glycans	affinity purified immunoglobulin G			enzymatically released N-glycans	Cterm		affinity purified immunoglobulin G			We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).
32454127	8	79	gly	glycosylated	1365:1376	arg1	highly glycosylated PrP	highly glycosylated PrP				PUBTATOR		PrP	19122		Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.
32045434	0	47	gly	hyperglycosylation	18:35	arg1	human chorionic gonadotropin	human chorionic gonadotropin				OGER		chorionic gonadotropin			Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis.
32517158	1	53	gly	glycoprotein	136:147	arg1	Endomucin	Endomucin				PUBTATOR		Endomucin	59308		Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.
33087860	3	38	gly	MACs	446:449	arg1	Host-derived mucus glycans	MACs			Host-derived mucus glycans	OGER		MACs	P29966		Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.
33087860	3	61	gly	glycans	364:370	arg1	gut-secreted mucin proteins	mucin proteins			glycans	PUBTATOR		mucin proteins	100508689		Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.
34369795	7	1	gly	distribution	1075:1086	arg1	the coronaviruses' S1 protein	S1 protein			distribution	Cterm		S1 protein	7448		This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.
32928962	3	58	gly	glycans	465:471	arg1	lubricin	lubricin			glycans	PUBTATOR		lubricin	10216		The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.
32049016	3	28	gly	contain	448:454	arg1	Env gp120 AND O-linked carbohydrate	Env gp120		the variable 1 (V1) domain	O-linked carbohydrate	PUBTATOR		Env gp120	100616444	domain	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.
34543117	7	18	gly	pigs	1624:1627	arg1	sialic acid-containing structures	pigs			sialic acid-containing structures	OGER		pigs	Q96S52		Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.
33231436	7	17	gly	glycoform	1246:1254	arg1	the EPO glycoform	the EPO glycoform				PUBTATOR		EPO	13856		The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.
32438810	2	52	gly	glycoproteins	311:323	arg1	immunoglobulin-G	immunoglobulin-G				Cterm		immunoglobulin-G (Ig			In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).
32438810	2	52	gly	glycoproteins	311:323	arg1	lactoferrin	lactoferrin				PUBTATOR		lactoferrin	280846		In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).
32438810	2	52	gly	glycoproteins	311:323	arg1	glycosylation-dependent cellular adhesion molecule 1	glycosylation-dependent cellular adhesion molecule 1				PUBTATOR		glycosylation-dependent cellular adhesion molecule 1	282430		In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).
32438810	2	52	gly	glycoproteins	311:323	arg1	lactoperoxidase	lactoperoxidase				PUBTATOR		lactoperoxidase	280844		In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).
32438810	2	52	gly	glycoproteins	311:323	arg1	α-lactalbumin	α-lactalbumin				PUBTATOR		-lactalbumin	281894		In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).
32045434	15	82	gly	carries	2011:2017	arg1	hCG AND the same N-glycans	hCG			the same N-glycans	OGER		hCG			hCG carries the same N-glycans throughout pregnancy but in different proportions.
33954318	3	15	gly	fucosylated	357:367	arg1	Especially fucosylated hMOs	Especially fucosylated hMOs				PUBTATOR		hMOs	4342		Especially fucosylated hMOs have received attention for their anti-adhesive effects on pathogens, preventing attachment to the intestine and infection.
32929138	3	28	gly	glycoprotein	679:690	arg1	the S glycoprotein	the S glycoprotein				Cterm		S glycoprotein	43740568		These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.
31941772	1	3	gly	glycoprotein	158:169	arg1	Env	Env				PUBTATOR		Env			Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.
34044019	8	67	gly	fucose	1604:1609	arg1	IgG1	IgG1			fucose	OGER		IgG1	P01857		The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.
32623356	13	41	gly	glycosylated	2027:2038	arg1	glycosylated HMGB1	glycosylated HMGB1				PUBTATOR		HMGB1	3146		The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.
32546343	7	42	gly	glycoforms	1188:1197	arg1	IgA	IgA			glycoforms	OGER		IgA	P11912		PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.
33876698	12	40	gly	glycoforms	1725:1734	arg1	PPT1	PPT1				PUBTATOR		PPT1	5538		These N-glycans do not impede drug binding, thus suggesting that all glycoforms of PPT1 can be targeted with these compounds.Communicated by Ramaswamy H. Sarma.
33758835	5	17	gly	glycosylated	772:783	arg1	a fully glycosylated spike	a fully glycosylated spike				PUBTATOR		spike	43740568		Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.
34523784	0	52	gly	Erythropoietin	67:80	arg1	Sialylated Glycoforms	Human Erythropoietin			Sialylated Glycoforms	OGER		Human Erythropoietin	P01588		Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.
35178379	2	61	gly	glycosylated	242:253	arg1	The SARS-CoV-2 spike protein	The SARS-CoV-2 spike protein				PUBTATOR		spike protein	43740568		The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.
34056088	2	47	gly	glycoprotein	329:340	arg1	the viral trimeric spike glycoprotein	the viral trimeric spike glycoprotein				PUBTATOR		spike glycoprotein	43740568		Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.
32161592	4	31	gly	MGL	581:583	arg1	Tn antigen-mediated activation	MGL			Tn antigen-mediated activation	PUBTATOR		MGL	Q99685		Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.
32169901	5	45	gly	glycosylation	1409:1421	arg1	PSP	PSP				OGER		PSP	O60542		We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.
32169901	5	45	gly	glycosylation	1409:1421	arg1	LTA	LTA				OGER		LTA	P01374		We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.
33084194	2	44	gly	contains	286:293	arg1	LAP AND carbohydrates	LAP			carbohydrates	PUBTATOR		LAP	P11117		LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).
34440649	3	81	gly	AGPs	627:630	arg1	the carbohydrate chains	AGPs			the carbohydrate chains	OGER		AGPs	O00116		In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.
32513872	7	79	gly	glycosylated	1863:1874	arg1	incompletely glycosylated PrP	incompletely glycosylated PrP				OGER		PrP	P32119		Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.
33756033	6	4	gly	IL-6	853:856	arg1	the 2,6-sialylated glycoforms	IL-6			the 2,6-sialylated glycoforms	PUBTATOR		IL-6	24498		The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.
33756033	6	15	gly	glycoforms	839:848	arg1	IL-6	IL-6				PUBTATOR		IL-6	24498		The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.
33756033	6	23	gly	2,6-sialylated	824:837	arg1	IL-6	IL-6				PUBTATOR		IL-6	24498		The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.
32568201	10	19	gly	glycosylation	1416:1428	arg1	CSF	CSF				OGER		CSF			The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.
34726315	6	16	gly	sugars	1181:1186	arg1	glcat14a/b	glcat14a/b			sugars	PUBTATOR		glcat14a/b	833996		Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.
33861503	2	51	gly	LGP	468:470	arg1	The monosaccharide composition	LGP			The monosaccharide composition	PUBTATOR		LGP	57426		The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.
33861503	2	51	gly	LGP	468:470	arg1	galactose	LGP			galactose	PUBTATOR		LGP	57426		The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.
33459939	1	18	gly	glycans	186:192	arg1	α2,6-linkage	2			glycans	PUBTATOR		2	170589		Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.
33459939	1	18	gly	glycans	186:192	arg1	α2,3-	2,3-			glycans	PUBTATOR		2,3-	28923		Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.
33065002	6	27	gly	glycosylated	853:864	arg1	the glycosylated Vo subunits	the glycosylated Vo subunits				Cterm		Vo subunits	537		The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.
33876698	10	19	gly	N-glycosylated	1466:1479	arg1	PPT1	PPT1				PUBTATOR		PPT1	5538		N-glycosylated forms of PPT1 were elaborated.
32623356	5	89	gly	N-glycosylated	659:672	arg1	HMGB1	HMGB1				PUBTATOR		HMGB1	3146		In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.
32125558	3	7	gly	2,6	606:608	arg1	two N-acetylneuraminic acid-based key enzymes	(2,6)-sialyltransferase			two N-acetylneuraminic acid-based key enzymes	PUBTATOR		(2,6)-sialyltransferase	100689389		Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.
33141553	5	16	gly	glycoprotein	916:927	arg1	clusterin	clusterin				PUBTATOR		clusterin	1191		We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.
32005658	6	15	gly	modified	1356:1363	arg3	HEK-sKlotho AND an unusual N-glycan structure	HEK-sKlotho			an unusual N-glycan structure	OGER		HEK	P29320		MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.
31907284	1	13	gly	glycoproteins	145:157	arg1	glycoproteins	glycoproteins				PUBTATOR		e glycoproteins	51428		Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.
31907284	1	13	gly	glycoproteins	145:157	arg1	Abs	Abs				OGER		Abs	Q9UJV9		Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.
31907284	1	39	gly	attached	204:211	arg1	the Fc AND a conserved N-linked carbohydrate	the Fc			a conserved N-linked carbohydrate	PUBTATOR		e Fc	51428		Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.
31907284	1	60	gly	carry	164:168	arg1	glycoproteins AND a conserved N-linked carbohydrate	glycoproteins			a conserved N-linked carbohydrate	PUBTATOR		e glycoproteins	51428		Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.
31907284	1	60	gly	carry	164:168	arg1	Abs AND a conserved N-linked carbohydrate	Abs			a conserved N-linked carbohydrate	OGER		Abs	Q9UJV9		Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.
32788422	5	63	gly	N-glycosylation	611:625	arg1	IgG	IgG				PUBTATOR		IgG	668542		Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.
34056088	5	3	gly	glycoproteins	919:931	arg1	engineered spike glycoproteins	engineered spike glycoproteins				PUBTATOR		spike glycoproteins	43740568		Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.
33650863	6	81	gly	UMOD	1357:1360	arg1	comprehensive N-glycan profiling	UMOD			comprehensive N-glycan profiling	PUBTATOR		UMOD	7369		To our knowledge, this is the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS.
34229018	4	56	gly	→	938:938	arg1	SCP	SCP			→	OGER		SCP	Q9BRV3		The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.
32280962	0	42	gly	glycoprotein	46:57	arg1	pregnancy-specific glycoprotein 1	pregnancy-specific glycoprotein 1			Glycan characterization	OGER		pregnancy-specific glycoprotein 1	P11464		Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.
33876698	9	53	part_of	PPT1	1460:1463	arg1	site Met112	PPT1		site Met112		PUBTATOR	AminoAcid	PPT1	5538	Met112	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.
32678978	4	45	gly	glycosylated	1024:1035	arg1	S-protein	S-protein				PUBTATOR		S-protein	7448		Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.
32678978	4	45	gly	glycosylated	1024:1035	arg1	ACE2	ACE2				PUBTATOR		ACE2	59272		Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.
32678978	4	45	gly	glycosylated	1024:1035	arg1	SARS-CoV-2 spike protein	SARS-CoV-2 spike protein				PUBTATOR		spike protein	43740568		Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.
34582712	3	5	gly	glycosylated	398:409	arg1	The α-DG subunit	The α-DG subunit				Cterm		DG subunit	Q14118		The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.
33631789	11	99	gly	Fc-glycosylation	2052:2067	arg1	anti-TNF-alpha Abs	anti-TNF-alpha Abs				PUBTATOR		TNF-alpha Abs	7124		CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.
34494876	14	110	gly	ACE2	2204:2207	arg1	N-glycans	ACE2			N-glycans	PUBTATOR		ACE2	Q9BYF1		In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.
34424752	6	2	gly	RNase	1246:1250	arg1	a model high-mannose-type glycoprotein	RNase B			a model high-mannose-type glycoprotein	OGER		RNase B	P07998		Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.
34424752	6	18	gly	glycoprotein	1281:1292	arg1	RNase B	RNase B				OGER		RNase B	P07998		Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.
34051740	4	108	gly	AGPs	768:771	arg1	the glucuronic acid residues	AGPs			the glucuronic acid residues	OGER		AGPs	O00116		One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.
34051740	4	108	gly	AGPs	768:771	arg1	One such sugar residue	AGPs			One such sugar residue	OGER		AGPs	O00116		One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.
32904601	7	9	gly	hACE2	1190:1194	arg1	The PTM and glycan maps	hACE2			The PTM and glycan maps	PUBTATOR		hACE2	59272		The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.
32904601	7	74	gly	protein	1178:1184	arg1	The PTM and glycan maps	HCoV-19 S protein			The PTM and glycan maps	OGER		HCoV-19 S protein	Q15517		The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.
33675758	10	80	gly	deglycosylated	1489:1502	arg1	deglycosylated IgG1	deglycosylated IgG1				OGER		IgG1	P01857		To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.
34067878	11	25	gly	glycans	1594:1600	arg1	ACE2	ACE2			glycans	PUBTATOR		ACE2	Q9BYF1		Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.
34051740	1	68	gly	glycosylated	224:235	arg1	AGPs	AGPs				OGER		AGPs	O00116		BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.
33566602	5	14	gly	N-glycans	854:862	arg1	Mucin-5B	Mucin-5B			N-glycans	PUBTATOR		Mucin-5B	Q9HC84		Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.
34494876	16	59	gly	ACE2	2549:2552	arg1	glycan structure	ACE2			glycan structure	PUBTATOR		ACE2	Q9BYF1		These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.
33124956	6	10	gly	glycosylation	891:903	arg1	IL-1RI	IL-1RI				PUBTATOR		IL-1RI	3554		Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.
33124956	6	10	gly	glycosylation	891:903	arg1	IL-1RAcP	IL-1RAcP				PUBTATOR		IL-1RAcP	3556		Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.
33992099	9	27	gly	L-selectin	1542:1551	arg1	MECA-79 glycans	L-selectin			MECA-79 glycans	OGER		L-selectin	P14151		RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.
33756033	5	54	gly	glycoform	645:653	arg1	Each IL-6 glycoform	Each IL-6 glycoform				PUBTATOR		IL-6	24498		Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan.
33688648	2	4	gly	found	466:470	arg2	S-RBD AND the new O-glycans	S-RBD			the new O-glycans	PUBTATOR		S	43740568		However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.
34494876	9	117	gly	deglycosylation	1355:1369	arg1	ACE2	ACE2				PUBTATOR		ACE2	Q9BYF1		Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.
34494876	9	117	gly	deglycosylation	1355:1369	arg1	S proteins	S proteins				Cterm		S proteins	43740568		Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.
34056088	3	60	gly	glycoprotein	564:575	arg1	spike glycoprotein production	spike glycoprotein production				PUBTATOR		spike glycoprotein	43740568		Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.
34335667	4	9	gly	GAA	740:742	arg1	The N-glycan composition	GAA			The N-glycan composition	PUBTATOR		GAA	2548		The N-glycan composition of the GAA consisted of a predominantly paucimannosidic structure, Man3GlcNAc2 (M3), without the plant-specific N-glycans.
32109354	1	21	gly	protein	145:151	arg1	The high mannose patch	HIV envelope protein			The high mannose patch	PUBTATOR		HIV envelope protein	64006		The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.
32280962	1	35	gly	glycoprotein	140:151	arg1	Pregnancy-specific beta 1 glycoprotein	Pregnancy-specific beta 1 glycoprotein				PUBTATOR		Pregnancy-specific beta 1 glycoprotein	653492		Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.
32280962	1	35	gly	glycoprotein	140:151	arg1	PSG1	PSG1				PUBTATOR		PSG1	5669		Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.
34823411	7	13	gly	chain	1231:1235	arg1	a single α-D-Glcp branch	chain			a single α-D-Glcp branch	OGER		chain	77683		The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.
33231436	5	84	gly	glycoform	787:795	arg1	EPO	EPO				PUBTATOR		EPO	13856		Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.
33231436	5	84	gly	glycoform	787:795	arg1	an erythropoietin	an erythropoietin				PUBTATOR		erythropoietin	13856		Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.
33756033	4	5	gly	glycoforms	475:484	arg1	Nine IL-6 glycoforms	Nine IL-6 glycoforms				PUBTATOR		IL-6	24498		Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.
33756033	4	41	gly	non-glycosylated	587:602	arg1	non-glycosylated recombinant IL-6	non-glycosylated recombinant IL-6				PUBTATOR		IL-6	24498		Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.
34582712	4	38	gly	α-DG	628:631	arg1	the carbohydrate structure	DG			the carbohydrate structure	Cterm		DG	Q14118		Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.
34876606	4	27	gly	N-glycosylation	773:787	arg1	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins				PUBTATOR		spike glycoproteins	43740568		In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.
34876606	4	57	gly	glycoproteins	852:864	arg1	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins				PUBTATOR		spike glycoproteins	43740568		In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.
34248947	2	56	gly	glycosylation	273:285	arg1	serum IgG	serum IgG				PUBTATOR		IgG	668542		This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.
32501643	9	69	part_of	APOE	1151:1154	arg1	serine 94	APOE		serine 94, threonine 194, and threonine 289		PUBTATOR	SpecificSite	APOE	348	serine 94, threonine 194, and threonine 289	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.
32501643	9	69	part_of	APOE	1151:1154	arg1	threonine 194	APOE		serine 94, threonine 194, and threonine 289		PUBTATOR	SpecificSite	APOE	348	serine 94, threonine 194, and threonine 289	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.
32501643	9	69	part_of	APOE	1151:1154	arg1	threonine 194	APOE		serine 94, threonine 194, and threonine 289		PUBTATOR	SpecificSite	APOE	348	serine 94, threonine 194, and threonine 289	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.
34669426	1	9	gly	glycoprotein	184:195	arg1	Env	Env				PUBTATOR		Env	100616444		The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.
34669426	1	9	gly	glycoprotein	184:195	arg1	trimeric envelope glycoprotein	trimeric envelope glycoprotein				PUBTATOR		envelope glycoprotein	100616444		The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.
33940104	2	54	gly	sugar	437:441	arg1	PSF	PSF			sugar	OGER		PSF			Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.
32454127	3	1	gly	glycans	464:470	arg1	PrP	PrP			glycans	PUBTATOR		PrP	19122		In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.
32479947	4	27	gly	PSP	655:657	arg1	monosaccharide composition	PSP			monosaccharide composition	OGER		PSP	O60542		Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.
33758080	0	27	gly	O-glycosylation	23:37	arg1	chemoattractant receptor GPR15	chemoattractant receptor GPR15				PUBTATOR		GPR15	2838		Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.
32544320	5	60	gly	glycoprotein	808:819	arg1	AGP	AGP				PUBTATOR		AGP	497200		The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.
32544320	5	60	gly	glycoprotein	808:819	arg1	alpha-1 acid glycoprotein	alpha-1 acid glycoprotein				PUBTATOR		alpha-1 acid glycoprotein	497200		The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.
32172840	3	28	gly	COP-W1	406:411	arg1	mannose	COP			mannose	OGER		COP			The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.
32172840	3	28	gly	COP-W1	406:411	arg1	The monosaccharide composition	COP			The monosaccharide composition	OGER		COP			The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.
33140034	4	12	gly	glycosylated	627:638	arg1	the glycosylated SARS-CoV-2 S protein	the glycosylated SARS-CoV-2 S protein				PUBTATOR		S protein	Q15517		Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.
33077685	1	21	gly	spike	106:110	arg1	a determinant	spike			a determinant	PUBTATOR		spike	43740568		The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.
32767150	6	34	gly	variants	821:828	arg1	the glycan structures	CGB variants			the glycan structures	PUBTATOR		CGB variants	93659		Here, we investigate the glycan structures and possible functional differences of the two CGB variants.