doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_base_np	arg_protein	arg_domain	arg_site	arg_sugar	PSource	SiteSource	NProtein	NID	SiteName	sent_text
34229070	7	24	gly	sialylated	1245:1254	arg1	sialylated O-glycans				sialylated O-glycans						We found a dense cluster of truncated O-glycans on the region nearby beginning of E2 domain and high abundance of sialylated O-glycans on the region close to β-cleavage site.
34229070	7	45	gly	region	1273:1278	arg1	sialylated O-glycans			region	sialylated O-glycans					region	We found a dense cluster of truncated O-glycans on the region nearby beginning of E2 domain and high abundance of sialylated O-glycans on the region close to β-cleavage site.
34229070	7	98	gly	O-glycans	1256:1264	arg1	the region			the region	the region		Site			region	We found a dense cluster of truncated O-glycans on the region nearby beginning of E2 domain and high abundance of sialylated O-glycans on the region close to β-cleavage site.
31336250	5	5	gly	glycosylated	1181:1192	arg1	wild-type glycosylated Fc	wild-type glycosylated Fc				Cterm		Fc			High-throughput screening of the resulting library led to the identification of an aglycosylated Fc variant that exhibited almost double the antibody-dependent cell-mediated cytotoxicity than wild-type glycosylated Fc.
31336250	5	35	gly	aglycosylated	1062:1074	arg1	an aglycosylated Fc variant	an aglycosylated Fc variant				Fterm		variant			High-throughput screening of the resulting library led to the identification of an aglycosylated Fc variant that exhibited almost double the antibody-dependent cell-mediated cytotoxicity than wild-type glycosylated Fc.
32030495	0	76	gly	N-glycoprotein	55:68	arg1	N-glycoprotein	N-glycoprotein				Fterm		N-glycoprotein			A quantitative N-glycoproteomics study of cell-surface N-glycoprotein markers of MCF-7/ADR cancer stem cells.
32109505	0	81	gly	N-glycosylation	0:14	arg1	TRPM8 protein	TRPM8 protein				PUBTATOR		TRPM8 protein	79054		N-glycosylation state of TRPM8 protein revealed by terahertz spectroscopy and molecular modelling.
33340519	3	36	gly	glycosylated	548:559	arg1	angiotensin-converting enzyme 2	angiotensin-converting enzyme 2				OGER		angiotensin-converting enzyme 2	Q9BYF1		The viral spike glycoprotein (S) of SARS-CoV-2 and the human cellular receptor, angiotensin-converting enzyme 2 (ACE2) are both densely glycosylated.
33340519	3	36	gly	glycosylated	548:559	arg1	the human cellular receptor	the human cellular receptor				Fterm		receptor			The viral spike glycoprotein (S) of SARS-CoV-2 and the human cellular receptor, angiotensin-converting enzyme 2 (ACE2) are both densely glycosylated.
33340519	3	48	gly	glycoprotein	428:439	arg1	viral spike glycoprotein	viral spike glycoprotein				Fterm		glycoprotein			The viral spike glycoprotein (S) of SARS-CoV-2 and the human cellular receptor, angiotensin-converting enzyme 2 (ACE2) are both densely glycosylated.
33664361	7	64	gly	N-glycosylated	1112:1125	arg1	the ZIKV E protein	protein		site		Fterm		protein		site	This confirmed that this site of the ZIKV E protein is highly N-glycosylated but with very high micro-heterogeneity.
29755357	0	38	gly	N-Glycosylation	0:14	arg1	Lipocalin 2	Lipocalin 2				PUBTATOR		Lipocalin 2	3934		N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting.
31156631	8	42	gly	deglycosylation	828:842	arg1	N-linked α-gal-containing proteins				N-linked α-gal-containing proteins						PNGase F treatment confirmed the deglycosylation of N-linked α-gal-containing proteins in tick salivary glands.
28922609	12	12	gly	FAC	2195:2197	arg1	secreted, glycosylation site			secreted, glycosylation site						site	Conversely, disulfide bond was unique to FAC; secreted, glycosylation site: N-linked, glycosylation, glycoprotein, polymorphism, and sequence variant were associated solely with FAP.
28922609	12	76	gly	glycoprotein	2255:2266	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Conversely, disulfide bond was unique to FAC; secreted, glycosylation site: N-linked, glycosylation, glycoprotein, polymorphism, and sequence variant were associated solely with FAP.
29784879	0	22	gly	protein	117:123	arg1	the mannose-trimming activity	ER degradation-enhancing α-mannosidase-like protein 3			the mannose-trimming activity	PUBTATOR		ER degradation-enhancing α-mannosidase-like protein 3	80267		ER-resident protein 46 (ERp46) triggers the mannose-trimming activity of ER degradation-enhancing α-mannosidase-like protein 3 (EDEM3).
32001344	6	67	gly	glycosylation	986:998	arg1	the protein	the protein				Fterm		protein			We further show that N-linked glycosylation is required for efficient secretion and solubility of the protein.
31275257	1	32	gly	origin	285:290	arg1	dietary glycans	origin			dietary glycans	Fterm		origin			The human Intestinal mucus is formed by glycoproteins, the O- and N-linked glycans which constitute a crucial source of carbon for commensal gut bacteria, especially when deprived of dietary glycans of plant origin.
31275257	1	73	gly	glycoproteins	117:129	arg1	glycoproteins	glycoproteins			O-	Fterm		glycoproteins			The human Intestinal mucus is formed by glycoproteins, the O- and N-linked glycans which constitute a crucial source of carbon for commensal gut bacteria, especially when deprived of dietary glycans of plant origin.
31275257	1	73	gly	glycoproteins	117:129	arg1	glycoproteins	glycoproteins			N-linked glycans	Fterm		glycoproteins			The human Intestinal mucus is formed by glycoproteins, the O- and N-linked glycans which constitute a crucial source of carbon for commensal gut bacteria, especially when deprived of dietary glycans of plant origin.
31498587	5	16	gly	glycopeptides	676:688	arg2	MUC1-Tf and -STn glycopeptides			MUC1-Tf and -STn glycopeptides						glycopeptides	The conjugates of MUC1-Tf and -STn glycopeptides with Qβ were utilized to immunize immune-tolerant human MUC1 transgenic (MUC1.Tg) mice, which elicited superior levels of anti-MUC1 IgG antibodies with titers reaching over 2 million units.
30476078	8	107	gly	sialylation	1657:1667	arg1	the MC38-glycovariants	MC38-glycovariants			sialylation	Fterm		MC38-glycovariants			Moreover, Lewisx expression was found to influence core-fucosylation, sialylation, antennarity and the subtypes of N-glycans in the MC38-glycovariants.
30476078	8	103	gly	core-fucosylation	1638:1654	arg1	the MC38-glycovariants	MC38-glycovariants			core-fucosylation	Fterm		MC38-glycovariants			Moreover, Lewisx expression was found to influence core-fucosylation, sialylation, antennarity and the subtypes of N-glycans in the MC38-glycovariants.
30476078	8	103	gly	core-fucosylation	1638:1654	arg1	the MC38-glycovariants	MC38-glycovariants			N-glycans	Fterm		MC38-glycovariants			Moreover, Lewisx expression was found to influence core-fucosylation, sialylation, antennarity and the subtypes of N-glycans in the MC38-glycovariants.
30476078	8	107	gly	sialylation	1657:1667	arg1	the MC38-glycovariants	MC38-glycovariants			N-glycans	Fterm		MC38-glycovariants			Moreover, Lewisx expression was found to influence core-fucosylation, sialylation, antennarity and the subtypes of N-glycans in the MC38-glycovariants.
31511323	0	80	gly	N-Glycosylation	0:14	arg1	the voltage-gated sodium channel β2 subunit	the voltage-gated sodium channel β2 subunit				PUBTATOR		2 subunit	170589		N-Glycosylation of the voltage-gated sodium channel β2 subunit is required for efficient trafficking of NaV1.5/β2 to the plasma membrane.
28490633	11	56	gly	glycoproteins	1717:1729	arg1	misfolded glycoproteins	misfolded glycoproteins				Fterm		glycoproteins			In conclusion, we have determined the UGGT structure, which enabled us to develop a plausible functional model of the mechanism for UGGT's selectivity for misfolded glycoproteins.
29237830	7	42	gly	glycoprotein	1085:1096	arg1	LASV glycoprotein	LASV glycoprotein				Fterm		glycoprotein			To study LASV receptor use in the context of productive arenavirus infection, we employed recombinant lymphocytic choriomeningitis virus expressing LASV glycoprotein (rLCMV-LASV GP) as a validated biosafety level 2 (BSL2) model.
30944176	4	33	gly	glycosylation	567:579	arg2	this glycosylation site			this glycosylation site						site	All contemporary ZIKV isolates encode an N-linked glycosylation site in the envelope (E) protein (N154), but this glycosylation site is absent in many historical ZIKV isolates.
30944176	4	63	gly	glycosylation	503:515	arg2	an N-linked glycosylation site			an N-linked glycosylation site						site	All contemporary ZIKV isolates encode an N-linked glycosylation site in the envelope (E) protein (N154), but this glycosylation site is absent in many historical ZIKV isolates.
30550553	1	59	gly	motif	174:178	arg1	two phenylalanines			two phenylalanines						phenylalanines	The glycolipid transfer protein, GLTP, can be found in the cytoplasm, and it has a FFAT-like motif (two phenylalanines in an acidic tract) that targets it to the endoplasmic reticulum (ER).
34257876	10	22	gly	glycosylation	1552:1564	arg2	the site			the site						site	With this approach, information on the site of glycosylation, the glycoform, the source protein, and the target protein of the cross-linked pair were obtained.
30523150	1	61	gly	levels	233:238	arg1	cellular proteins	proteins			levels	Fterm		proteins			The addition of a single β-d-GlcNAc sugar (O-GlcNAc) by O-GlcNAc-transferase (OGT) and O-GlcNAc removal by O-GlcNAcase (OGA) maintain homeostatic O-GlcNAc levels on cellular proteins.
32965048	7	51	gly	N-glycopeptides	1433:1447	arg2	2986 intact N-glycopeptides			2986 intact N-glycopeptides						N-glycopeptides	RESULTS We identified 2986 intact N-glycopeptides with comprehensive site and structure information for the peptide backbones (amino acid sequences and N-glycosites) and the N-glycan moieties (monosaccharide compositions, sequences/linkages).
32965048	7	83	gly	N-glycosites	1551:1562	arg2	N-glycosites			N-glycosites						sequences and N-glycosites	RESULTS We identified 2986 intact N-glycopeptides with comprehensive site and structure information for the peptide backbones (amino acid sequences and N-glycosites) and the N-glycan moieties (monosaccharide compositions, sequences/linkages).
32965048	7	111	gly	backbones	1515:1523	arg1	amino acid sequences			sequences and N-glycosites						sequences and N-glycosites	RESULTS We identified 2986 intact N-glycopeptides with comprehensive site and structure information for the peptide backbones (amino acid sequences and N-glycosites) and the N-glycan moieties (monosaccharide compositions, sequences/linkages).
27343203	4	81	gly	glycosylation	725:737	arg2	the N-linked glycosylation site			the N-linked glycosylation site						site	One variant comprised the full extracellular portion of CD23, including the stalk and head domain; 1 variant was identical with the first, except for an amino acid exchange in the stalk region abolishing the N-linked glycosylation site; and 2 variants represented the head domain, 1 complete and 1 truncated.
32374435	8	42	gly	glycoprotein	1706:1717	arg1	safe glycoprotein pharmaceutical production	safe glycoprotein pharmaceutical production				Fterm		glycoprotein			Our findings reinforce accumulating evidence that robustly customizing the N-glycosylation pathway in P. pastoris to produce particular human-type structures is still an incompletely solved synthetic biology challenge, which will require further innovation to enable safe glycoprotein pharmaceutical production.
29339411	5	27	gly	glycosylation	1056:1068	arg2	the multiple sites			the multiple sites						sites	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Ser182			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Thr149			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Thr259			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Ser334			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Thr149			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Thr259			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Ser334			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Thr259			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Ser334			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
29339411	5	6	gly	protein	1135:1141	arg1	Ser334			Thr149, Ser182, Thr199, Thr259, and Ser334				Selenomonas		Thr149, Ser182, Thr199, Thr259, and Ser334	From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334), the only flagellin protein identified.
32699088	4	52	gly	glycosylation	692:704	arg2	head glycosylation sites			head glycosylation sites						sites	Variable sites are almost exclusive to H1N1 IAVs of human origin, where the number of head glycosylation sites first increased over time and then decreased with and after the introduction of the 2009 pandemic H1N1 IAV of Eurasian swine origin.
32109505	5	66	gly	glycosylation	681:693	arg2	the sole glycosylation site			the sole glycosylation site						site	Here, experimental data performed with deglycosylating agents assess that the sole glycosylation site contains complex glycans with a molecular weight of 2.5 kDa.
32109505	5	35	gly	contains	700:707	arg1	the sole glycosylation site AND complex glycans			the sole glycosylation site	complex glycans					site	Here, experimental data performed with deglycosylating agents assess that the sole glycosylation site contains complex glycans with a molecular weight of 2.5 kDa.
28528272	8	61	gly	cystatin	1218:1225	arg1	complex mannose-linked	cystatin E/M			complex mannose-linked	PUBTATOR		cystatin E/M	1474		The carbohydrates on legumain were shown to be of the hybrid or high mannose type, whereas cystatin E/M was characterized as complex mannose-linked.
28528272	8	95	gly	carbohydrates	1131:1143	arg1	legumain	legumain			carbohydrates	OGER		legumain	Q99538		The carbohydrates on legumain were shown to be of the hybrid or high mannose type, whereas cystatin E/M was characterized as complex mannose-linked.
32544330	5	16	gly	glycan	758:763	arg1	Asn88			Asn88	Asn88		AminoAcid	Ribonuclease 1	P07998	Asn88	In contrast, the glycan on Asn88 is flexible and can even enter the enzymic active site, hindering catalysis.
30029404	0	41	gly	glycopeptides	125:137	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	Preparation of iminodiacetic acid functionalized silica capillary trap column for on-column selective enrichment of N-linked glycopeptides.
29672582	3	62	gly	released	743:750	arg1	serum IgG4 AND N-glycan	serum IgG4			N-glycan	OGER		IgG4	P01861		This study determined the concentration of N-linked glycans (N-glycan) released from serum IgG4 in IgG4RD patients and compared the difference of glycosylation changes to those in healthy controls.
29672582	3	62	gly	released	743:750	arg2	serum IgG4 AND N-linked glycans	serum IgG4			N-linked glycans	OGER		IgG4	P01861		This study determined the concentration of N-linked glycans (N-glycan) released from serum IgG4 in IgG4RD patients and compared the difference of glycosylation changes to those in healthy controls.
33560857	4	19	gly	N-glycosylation	787:801	arg2	21 out of 28 potential N-glycosylation sites			21 out of 28 potential N-glycosylation sites						sites	The present study investigated CEA samples purified from human colon carcinoma and human liver metastases and enabled the characterization of 21 out of 28 potential N-glycosylation sites with respect to their occupancy.
30069741	4	40	gly	sialylated	719:728	arg1	sialylated proteins	sialylated proteins				Fterm		proteins			The precise replication of human glycans is challenging, owing to the absence of a pathway in plants for the synthesis of sialylated proteins and the necessary precursors, but this can now be achieved by the coordinated expression of multiple human enzymes.
34106099	2	63	gly	glycosylated	291:302	arg1	Many proteins	Many proteins				Fterm		proteins			Many proteins in the brain associated with the synapse and important for synaptic transmission are highly glycosylated and their glycosylation could be important for learning and memory related molecular processes and synaptic plasticity.
28679762	6	115	gly	glycosylated	1077:1088	arg1	an N-linked glycosylated protein	an N-linked glycosylated protein				Fterm		protein			While we find that AAVR is an N-linked glycosylated protein, this glycosylation is not a strict requirement for AAV2 binding or functional transduction.
28679762	6	115	gly	glycosylated	1077:1088	arg1	AAVR	AAVR				PUBTATOR		AAVR	79932		While we find that AAVR is an N-linked glycosylated protein, this glycosylation is not a strict requirement for AAV2 binding or functional transduction.
33177111	1	11	gly	glycoproteins	210:222	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Altered glycosylations, which are associated with expression and activities of glycosyltransferases, can dramatically affect the function of glycoproteins and modify the behavior of tumor cells.
30814666	8	35	gly	Mtb glycoproteins	975:991	arg1	Mtb glycoproteins	Mtb glycoproteins				Fterm		Mtb glycoproteins			Collectively, the bulk of Mtb glycoproteins are involved in cell envelope biosynthesis, fatty acid and lipid metabolism, two-component systems, and pathogen-host interaction that are either surface exposed or located in the cell wall.
29793953	12	8	gly	either	2357:2362	arg1	sites			sites						sites	NTCP introduced to HepG2 cells was glycosylated at two N-linked glycosylation sites, but mutating either or both sites failed to prevent infection by cell culture-derived HBV or to confer susceptibility to serum-derived HBV.
29793953	12	24	gly	glycosylation	2323:2335	arg2	two N-linked glycosylation sites			two N-linked glycosylation sites						sites	NTCP introduced to HepG2 cells was glycosylated at two N-linked glycosylation sites, but mutating either or both sites failed to prevent infection by cell culture-derived HBV or to confer susceptibility to serum-derived HBV.
29793953	12	25	gly	glycosylated	2294:2305	arg1	NTCP	NTCP		sites		PUBTATOR		NTCP	6554	sites	NTCP introduced to HepG2 cells was glycosylated at two N-linked glycosylation sites, but mutating either or both sites failed to prevent infection by cell culture-derived HBV or to confer susceptibility to serum-derived HBV.
31829588	1	13	gly	glycosylation	106:118	arg1	bovine lactoferrin	bovine lactoferrin				PUBTATOR		lactoferrin	280846		It has been reported previously that glycosylation of bovine lactoferrin changes over time.
30905461	3	79	gly	glycoproteins	294:306	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			SA in glycoproteins modulates a wide range of physiological and pathological processes and has been routinely measured in hospital since 1950s.
29980609	1	17	gly	glycoproteins	123:135	arg1	AICL glycoproteins	AICL glycoproteins				PUBTATOR		AICL glycoproteins	9976		AICL glycoproteins are cognate activation-induced ligands of the C-type lectin-like receptor NKp80, which is expressed on virtually all mature human NK cells, and NKp80-AICL interaction stimulates NK cell effector functions such as cytotoxicity and cytokine secretion.
29562282	8	47	gly	glycoprotein	1245:1256	arg1	a heavily O-GlcNAcylated and abundant nuclear pore glycoprotein	NUP62			a heavily O-GlcNAcylated and abundant nuclear pore glycoprotein	OGER		NUP62	P37198		Confocal imaging shows that AANL co-localizes extensively with NUP62, a heavily O-GlcNAcylated and abundant nuclear pore glycoprotein.
33578083	8	15	gly	glycosylated	1863:1874	arg1	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	We demonstrate the utility of these new software features by finding previously unrecognized glycopeptides in publicly available data, including glycosylated neuropeptides from rat brain.
33578083	8	79	gly	glycopeptides	1811:1823	arg2	previously unrecognized glycopeptides			previously unrecognized glycopeptides						glycopeptides	We demonstrate the utility of these new software features by finding previously unrecognized glycopeptides in publicly available data, including glycosylated neuropeptides from rat brain.
33578083	8	79	gly	glycopeptides	1811:1823	arg2	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	We demonstrate the utility of these new software features by finding previously unrecognized glycopeptides in publicly available data, including glycosylated neuropeptides from rat brain.
35518855	9	66	gly	fucosylated	1437:1447	arg1	the fucosylated N-glycans				the fucosylated N-glycans						And the abundance of the fucosylated N-glycans was increased from 40.9% (PCTs) to 48.3% (LUSC).
33205259	5	28	gly	glycosylation	920:932	arg2	site			site						site	Here we provide a perspective overview on the MS-based technologies relevant for biotherapeutic product characterisation with an emphasis on the recent developments that allow determination of glycosylation features such as site of glycosylation, sialic acid linkage, glycan structure, and content.
30487799	0	88	gly	IgM	60:62	arg1	Site-Specific N-Glycan Characterization	Grass Carp Serum IgM			Site-Specific N-Glycan Characterization	OGER		Grass Carp Serum IgM	Q15327		Site-Specific N-Glycan Characterization of Grass Carp Serum IgM.
34864596	10	31	gly	fucosylated	1698:1708	arg1	the mostly core fucosylated biantennary glycans				the mostly core fucosylated biantennary glycans						The results suggested no ADCC function - structure relationship due to the mostly core fucosylated biantennary glycans found.
34523671	3	13	gly	engineered	935:944	arg1	sequon			sequon						sequon	Using RP-HPLC with a novel phenyl bonded phase to resolve intact protein glycoforms, the effect of sequon mutation on O-glycosylation initiation was examined through in vitro modification of the naturally O-glycosylated human interferon α-2b, and a sequon engineered human growth hormone.
34523671	3	44	gly	O-glycosylated	884:897	arg1	the naturally O-glycosylated human interferon α-2b	the naturally O-glycosylated human interferon α-2b				PUBTATOR		interferon α-2b	3440		Using RP-HPLC with a novel phenyl bonded phase to resolve intact protein glycoforms, the effect of sequon mutation on O-glycosylation initiation was examined through in vitro modification of the naturally O-glycosylated human interferon α-2b, and a sequon engineered human growth hormone.
34523671	3	99	gly	glycoforms	752:761	arg1	intact protein glycoforms	intact protein glycoforms				Fterm		protein			Using RP-HPLC with a novel phenyl bonded phase to resolve intact protein glycoforms, the effect of sequon mutation on O-glycosylation initiation was examined through in vitro modification of the naturally O-glycosylated human interferon α-2b, and a sequon engineered human growth hormone.
31341641	11	42	gly	glycosylation	1730:1742	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Furthermore, we observed that the nucleotide composition of indels was distinct from the flanking sequence, with higher frequencies of G and lower frequencies of T. Indels affected N-linked glycosylation sites more often in V1 and V2 than expected by chance, consistent with positive selection on glycosylation patterns within these regions.
30178920	4	11	gly	glycopeptides	1041:1053	arg2	glycopeptides			glycopeptides						glycopeptides	The monolith is also employed to the digests mixture of BSA and IgG (50:1, m/m), indicating the high enrichment selectivity of glycopeptides even in the presence of a large interference ratio.
31787385	5	36	gly	glycoprotein	915:926	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			The SL-bioemulsifier produced by strain SL-1 was identified as a glycoprotein with stable emulsifying activity over a wide range of environmental conditions.
32559081	1	1	gly	glycosylated	171:182	arg1	a fully glycosylated full-length SARS-CoV-2 spike (S) protein	a fully glycosylated full-length SARS-CoV-2 spike (S) protein				Fterm		protein			This technical study describes all-atom modeling and simulation of a fully glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
32559081	1	47	gly	protein	217:223	arg1	all-atom modeling and simulation	protein			all-atom modeling and simulation	Fterm		protein			This technical study describes all-atom modeling and simulation of a fully glycosylated full-length SARS-CoV-2 spike (S) protein in a viral membrane.
30177911	9	9	gly	glycosylated	1589:1600	arg1	proteins	proteins				Fterm		proteins			In a more original way, our proteomic analyses, confirmed by sWGA-enrichment and click-chemistry, revealed that rhoptries, proteins necessary for invasion, are glycosylated.
32168410	14	70	gly	deglycosylation	1652:1666	arg1	the native protein	the native protein				Fterm		protein			Complete deglycosylation of the native protein was achieved by a deglycosylation workflow.
34885895	4	50	gly	glycosylated	566:577	arg1	Not all N-X-[S/T] sequons			Not all N-X-[S/T] sequons							Not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
30149081	6	4	gly	glycoproteins	973:985	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The hemagglutination activity of rHEL2 lectins was strongly inhibited by glycoproteins containing mucin-type O-glycans.
30149081	6	57	gly	containing	987:996	arg1	glycoproteins AND mucin-type O-glycans	glycoproteins			mucin-type O-glycans	Fterm		glycoproteins			The hemagglutination activity of rHEL2 lectins was strongly inhibited by glycoproteins containing mucin-type O-glycans.
32334683	8	1	gly	glycoproteins	1319:1331	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We show that FTIR spectra of glycoproteins provide a global but accurate fingerprint of the glycosylation profile.
33263330	9	37	gly	glycoproteins	1789:1801	arg1	homogeneous glycoproteins	homogeneous glycoproteins				Fterm		glycoproteins			It is proposed that the GlycoDelete cell line could serve as a valuable go-to option for the production of homogeneous glycoproteins and their complexes for structural studies by X-ray crystallography and cryo-electron microscopy.
34687007	6	25	gly	glycopeptides	1156:1168	arg2	glycopeptides			glycopeptides						glycopeptides	General principles and practical examples of the most often used LC methods for Ig purification are described, together with typical workflows for N- and O-glycan analysis on the level of free glycans, glycopeptides, subunits, or intact Igs.
29869806	3	27	gly	O-glycoproteins	780:794	arg1	O-glycoproteins	O-glycoproteins				Fterm		O-glycoproteins			Here, we illustrate the potential of integrating data from capillary electrophoresis of transferrin, two-dimensional electrophoresis of N- and O-glycoproteins, mass spectrometry analyses of total serum N-linked glycans and mucin core1 O-glycosylated apolipoprotein C-III for the determination of various culprit CDG gene mutations.
30619781	5	35	gly	glycosylation	854:866	arg1	the tumor necrosis factor receptor type 1-associated DEATH domain protein	the tumor necrosis factor receptor type 1-associated DEATH domain protein				PUBTATOR		tumor necrosis factor receptor type 1-associated DEATH domain protein	8717		Addition of these compounds to cultured mammalian cells was sufficient to inhibit NleB1 glycosylation of the tumor necrosis factor receptor type 1-associated DEATH domain protein.
30529011	7	48	gly	phosphoglycoprotein	815:833	arg1	The SIBLING, matrix extracellular phosphoglycoprotein	The SIBLING, matrix extracellular phosphoglycoprotein				OGER		SIBLING, matrix extracellular phosphoglycoprotein	Q9NQ76		The SIBLING, matrix extracellular phosphoglycoprotein with ASARM motif (MEPE) is highly overexpressed in both BSP-/- and DKO and may impair mineralization through liberation of its ASARM (Acidic Serine-Aspartate Rich MEPE associated) peptides.
30888807	9	40	gly	had	1415:1417	arg1	P25 and anatase NPs AND higher protein and polysaccharide affinities	NPs			higher protein and polysaccharide affinities	OGER		NPs	P0C0P6		P25 and anatase NPs had higher protein and polysaccharide affinities, while rutile NPs exhibited stronger attachment onto phospholipids.
33107910	3	52	gly	N-glycosylation	339:353	arg2	An N-glycosylation site			An N-glycosylation site						site	An N-glycosylation site was introduced at position 178 of the H chain constant region of adalimumab Fab through site-directed mutagenesis (H:L178N Fab), and the H:L178N Fab was produced in Pichia pastoris.
29746846	2	29	gly	distribution	357:368	arg1	different placental regions			different placental regions	different placental regions		Site			regions	Our aim was to determine how O-GlcNAc levels are affected by hyperglycemia and the O-GlcNAc distribution in different placental regions.
30445613	7	34	gly	N-glycosylated	1097:1110	arg1	an N-glycosylated protein database	an N-glycosylated protein database				Fterm		database			We further screened an N-glycosylated protein database of C. elegans, and identified 456 germline-expressed genes coding N-glycosylated proteins.
30445613	7	69	gly	N-glycosylated	1195:1208	arg1	N-glycosylated proteins	N-glycosylated proteins				Fterm		proteins			We further screened an N-glycosylated protein database of C. elegans, and identified 456 germline-expressed genes coding N-glycosylated proteins.
32409993	3	3	gly	N-glycans	528:536	arg1	a protein	protein			N-glycans	Fterm		protein			The studies have shown that many glycoproteins hold multiple N-glycans, yet little is known about the redundancy of N-glycans on a protein.
32409993	3	54	gly	glycoproteins	445:457	arg1	many glycoproteins	many glycoproteins				Fterm		glycoproteins			The studies have shown that many glycoproteins hold multiple N-glycans, yet little is known about the redundancy of N-glycans on a protein.
32298658	2	54	gly	glycosylation	362:374	arg2	gain glycosylation sites			gain glycosylation sites						sites	Additionally, human-adapted HAs gain glycosylation sites over time, although their biological function is poorly defined.
30040982	3	5	gly	site	416:419	arg1	RET	RET			site	PUBTATOR		RET	5979		Both GFRA1 and RET are membrane proteins which are N-glycosylated but no O-linked sialylation site on GFRA1 or RET has been reported.
30040982	3	5	gly	site	416:419	arg1	GFRA1	GFRA1			site	PUBTATOR		GFRA1	2674		Both GFRA1 and RET are membrane proteins which are N-glycosylated but no O-linked sialylation site on GFRA1 or RET has been reported.
30040982	3	24	gly	N-glycosylated	373:386	arg1	RET	RET				PUBTATOR		RET	5979		Both GFRA1 and RET are membrane proteins which are N-glycosylated but no O-linked sialylation site on GFRA1 or RET has been reported.
30040982	3	24	gly	N-glycosylated	373:386	arg1	membrane proteins	membrane proteins				Fterm		proteins			Both GFRA1 and RET are membrane proteins which are N-glycosylated but no O-linked sialylation site on GFRA1 or RET has been reported.
30040982	3	24	gly	N-glycosylated	373:386	arg1	GFRA1	GFRA1				PUBTATOR		Both GFRA1	2674		Both GFRA1 and RET are membrane proteins which are N-glycosylated but no O-linked sialylation site on GFRA1 or RET has been reported.
28254785	3	14	gly	modifications	509:521	arg1	Notch1 epidermal growth factor-like (EGF) domains 8 and 12			Notch1 epidermal growth factor-like (EGF) domains 8 and 12	Notch1 epidermal growth factor-like (EGF) domains 8 and 12		Site			domains	O-Linked fucose modifications on Notch1 epidermal growth factor-like (EGF) domains 8 and 12 engage the EGF3 and C2 domains of Jag1, respectively, and different Notch1 domains are favored in binding to Jag1 than those that bind to the Delta-like 4 ligand.
30042931	2	35	gly	glycosylation	440:452	arg2	two glycosylation sites			two glycosylation sites						sites	JEV E protein has one N-linked glycosylation site at N154 (G2 site), but the related dengue virus E protein has two glycosylation sites at N67 (G1 site) and N153 (G2 site).
30042931	2	85	gly	glycosylation	355:367	arg2	one N-linked glycosylation site			one N-linked glycosylation site						site	JEV E protein has one N-linked glycosylation site at N154 (G2 site), but the related dengue virus E protein has two glycosylation sites at N67 (G1 site) and N153 (G2 site).
34545811	2	4	gly	N-glycans	323:331	arg1	surface glycoproteins	glycoproteins			N-glycans	Fterm		glycoproteins			Here, we observed that N-glycans on surface glycoproteins of dendrites shift from immature to mature forms containing sialic acid in response to increased neuronal activation.
34545811	2	45	gly	glycoproteins	344:356	arg1	surface glycoproteins	surface glycoproteins				Fterm		glycoproteins			Here, we observed that N-glycans on surface glycoproteins of dendrites shift from immature to mature forms containing sialic acid in response to increased neuronal activation.
30514763	4	48	gly	glycosylation	790:802	arg2	three O-linked glycosylation sites			three O-linked glycosylation sites						sites	Using affinity-purified MIC2 and MS/MS analysis along with enzymatic digestion assays, we observed that at least seven C-linked and three O-linked glycosylation sites exist within MIC2, with >95% occupancy at these O-glycosylation sites.
30514763	4	59	gly	O-glycosylation	858:872	arg2	these O-glycosylation sites			these O-glycosylation sites						sites	Using affinity-purified MIC2 and MS/MS analysis along with enzymatic digestion assays, we observed that at least seven C-linked and three O-linked glycosylation sites exist within MIC2, with >95% occupancy at these O-glycosylation sites.
29864502	6	72	gly	glycosylation	1109:1121	arg2	a non-conserved N-linked glycosylation site			a non-conserved N-linked glycosylation site						site	Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor.
29886537	6	52	gly	sialylation	907:917	arg1	PrPSc	PrPSc				PUBTATOR		PrPSc	19122		For assessing sialylation status of PrPSc, we developed a reliable protocol that involves two-dimensional electrophoresis followed by Western blot (2D).
29886537	6	60	gly	PrPSc	929:933	arg1	sialylation status	PrPSc			sialylation status	PUBTATOR		PrPSc	19122		For assessing sialylation status of PrPSc, we developed a reliable protocol that involves two-dimensional electrophoresis followed by Western blot (2D).
33998969	1	1	gly	residue	287:293	arg1	AsnXxxThr/Ser sequon			AsnXxxThr/Ser sequon						sequon	N-glycosylation is a complex mechanism in which the carbohydrate molecules bind to the Asn amino acid in the N-glycan consensus sequence (AsnXxxThr/Ser sequon, where Xxx is any residue, excluding Pro).
33998969	1	40	gly	sequence	238:245	arg1	any residue			any residue						residue	N-glycosylation is a complex mechanism in which the carbohydrate molecules bind to the Asn amino acid in the N-glycan consensus sequence (AsnXxxThr/Ser sequon, where Xxx is any residue, excluding Pro).
30933973	9	83	gly	glycosylation	1826:1838	arg1	gp120	gp120		residues		OGER		gp120	Q14624	residues	Features predicting neutralization sensitivity or resistance included 26 surface-accessible residues in the VRC01 and CD4 binding footprints, the length of gp120, the length of Env, the number of cysteines in gp120, the number of cysteines in Env, and 4 potential N-linked glycosylation sites; the top features will be advanced to the primary sieve analysis.
30933973	9	83	gly	glycosylation	1826:1838	arg1	gp120	gp120		sites		OGER		gp120	Q14624	sites	Features predicting neutralization sensitivity or resistance included 26 surface-accessible residues in the VRC01 and CD4 binding footprints, the length of gp120, the length of Env, the number of cysteines in gp120, the number of cysteines in Env, and 4 potential N-linked glycosylation sites; the top features will be advanced to the primary sieve analysis.
31616924	6	83	gly	sialylated	996:1005	arg1	sialylated core 1 (Neu5Acα2-3Galβ1-3GalNAcα1-) glycosylation				sialylated core 1 (Neu5Acα2-3Galβ1-3GalNAcα1-) glycosylation						Plasma APOE held more abundant (20.5%) N-terminal (Thr8) sialylated core 1 (Neu5Acα2-3Galβ1-3GalNAcα1-) glycosylation compared to CSF APOE (0.1%).
28017896	13	32	gly	modified	2150:2157	arg3	Akt AND O-GlcNAcylation	Akt			O-GlcNAcylation	PUBTATOR		Akt	24185		We confirmed that Akt was modified by O-GlcNAcylation, and glucosamine pretreatment increased the O-GlcNAcylation of Akt.
28017896	13	46	gly	Akt	2241:2243	arg1	the O-GlcNAcylation	Akt			the O-GlcNAcylation	PUBTATOR		Akt	24185		We confirmed that Akt was modified by O-GlcNAcylation, and glucosamine pretreatment increased the O-GlcNAcylation of Akt.
34780171	8	58	gly	glycosylation	1815:1827	arg1	MET	ERBB2		MET		PUBTATOR		ERBB2	2064	MET	Without the immunoprecipitation step, the large-scale glycoproteomic atlas also reveals site-specific glycosylation of many druggable receptor proteins, such as EGFR, MET, ERBB2, ERBB3, AXL, and IGF1R.
34780171	8	58	gly	glycosylation	1815:1827	arg1	MET	IGF1R		MET		PUBTATOR		IGF1R	3480	MET	Without the immunoprecipitation step, the large-scale glycoproteomic atlas also reveals site-specific glycosylation of many druggable receptor proteins, such as EGFR, MET, ERBB2, ERBB3, AXL, and IGF1R.
34780171	8	58	gly	glycosylation	1815:1827	arg1	MET	EGFR		MET		PUBTATOR		EGFR	1956	MET	Without the immunoprecipitation step, the large-scale glycoproteomic atlas also reveals site-specific glycosylation of many druggable receptor proteins, such as EGFR, MET, ERBB2, ERBB3, AXL, and IGF1R.
34780171	8	58	gly	glycosylation	1815:1827	arg1	MET	ERBB3		MET		PUBTATOR		ERBB3	2065	MET	Without the immunoprecipitation step, the large-scale glycoproteomic atlas also reveals site-specific glycosylation of many druggable receptor proteins, such as EGFR, MET, ERBB2, ERBB3, AXL, and IGF1R.
34780171	8	58	gly	glycosylation	1815:1827	arg1	MET	AXL		MET		PUBTATOR		AXL	558	MET	Without the immunoprecipitation step, the large-scale glycoproteomic atlas also reveals site-specific glycosylation of many druggable receptor proteins, such as EGFR, MET, ERBB2, ERBB3, AXL, and IGF1R.
34780171	8	58	gly	glycosylation	1815:1827	arg1	MET	proteins		MET		Fterm		proteins		MET	Without the immunoprecipitation step, the large-scale glycoproteomic atlas also reveals site-specific glycosylation of many druggable receptor proteins, such as EGFR, MET, ERBB2, ERBB3, AXL, and IGF1R.
31139149	1	42	gly	proteins	194:201	arg1	The dynamic O-linked-N-acetylglucosamine posttranslational modification	proteins			The dynamic O-linked-N-acetylglucosamine posttranslational modification	Fterm		proteins			The dynamic O-linked-N-acetylglucosamine posttranslational modification of nucleocytoplasmic proteins has emerged as a key regulator of diverse cellular processes including several hallmarks of cancer.
31139149	1	71	gly	modification	160:171	arg1	nucleocytoplasmic proteins AND The dynamic O-linked-N-acetylglucosamine posttranslational modification	nucleocytoplasmic proteins			The dynamic O-linked-N-acetylglucosamine posttranslational modification	Fterm		proteins			The dynamic O-linked-N-acetylglucosamine posttranslational modification of nucleocytoplasmic proteins has emerged as a key regulator of diverse cellular processes including several hallmarks of cancer.
31336133	0	51	gly	hyper-N-glycosylated	98:117	arg1	a hyper-N-glycosylated variant	a hyper-N-glycosylated variant				Fterm		variant			Effect of ANITVNITV peptide fusion on the bioactivity and pharmacokinetics of human IFN-α2b and a hyper-N-glycosylated variant.
31967800	3	9	gly	N-glycopeptides	856:870	arg2	complex intact N-glycopeptides			complex intact N-glycopeptides						N-glycopeptides	With the recent availability of an independent NCEs setup, we found that the combination of 20%/30%/30% is better than 20%/30%/40%; in the analysis of complex intact N-glycopeptides enriched from gastric cancer tissues, total IDs with spectrum-level FDR ≤ 1%, site-specific IDs with site-determining fragment ions, and structure-specific IDs with structure-diagnostic fragment ions were increased by 42% (4,767 → 6,746), 57% (599 → 942), and 97% (1771 → 3495), respectively.
28511131	8	71	gly	glycosylation	1429:1441	arg2	N-linked glycosylation site (NGS) analysis			N-linked glycosylation site (NGS) analysis						site	N-linked glycosylation site (NGS) analysis revealed a novel potential NGS at GP5 amino acid position 59 in two of the subgroup 2 Henan isolates.
30581149	2	49	gly	allergenic	450:459	arg1	peptide-specific			peptide-specific						peptide-specific	OBJECTIVES To engineer recombinant glycoproteins resembling carbohydrate-specific IgE epitopes from venoms, pollen and mites which can discriminate carbohydrate-specific IgE from allergenic, peptide-specific IgE.
30581149	2	88	gly	glycoproteins	306:318	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			OBJECTIVES To engineer recombinant glycoproteins resembling carbohydrate-specific IgE epitopes from venoms, pollen and mites which can discriminate carbohydrate-specific IgE from allergenic, peptide-specific IgE.
30135544	5	34	gly	glycosylation	715:727	arg2	the glycosylation site			the glycosylation site						site	In contrast, PrP F198S, a pathological mutant with an altered residue within the glycosylation site, and an unglycosylated PrP mutant, N181D/N197D, primarily exist in the cytoplasm.
33606939	2	28	gly	glycosylation	242:254	arg1	viral proteins	viral proteins				Fterm		proteins			The extensive glycosylation of viral proteins almost certainly affects the antibody response, but the investigation of such effects is hampered by the huge range of structures and interactions of surface glycans through their inherent complexity and flexibility.
34331854	7	37	gly	glycoproteins	1099:1111	arg1	cross-linked N-linked glycoproteins	cross-linked N-linked glycoproteins				Fterm		glycoproteins			In addition, covalently cross-linked complexes could be isolated, and protein components of cross-linked N-linked glycoproteins were identified by proteomics analysis.
34662441	9	21	gly	glycoforms	1768:1777	arg1	PSMA glycoforms	PSMA glycoforms				OGER		PSMA	Q04609		It will hopefully stimulate further research into PSMA glycoforms in the context of tumor staging, noninvasive detection of prostate tumors, and the impact of glycoforms on physicochemical and enzymatic characteristics of PSMA in a tissue-specific manner.
28408270	1	59	gly	serine-rich	149:159	arg1	Pneumococcal serine-rich repeat protein			serine	Pneumococcal serine-rich repeat protein					serine	Pneumococcal serine-rich repeat protein (PsrP) is a glycoprotein that mediates Streptococcus pneumoniae attachment to lung cells and promotes biofilm formation.
28408270	1	14	gly	glycoprotein	188:199	arg1	Pneumococcal serine-rich repeat protein	glycoprotein			Pneumococcal serine-rich repeat protein	Fterm		glycoprotein			Pneumococcal serine-rich repeat protein (PsrP) is a glycoprotein that mediates Streptococcus pneumoniae attachment to lung cells and promotes biofilm formation.
28931878	1	48	gly	glycans	99:105	arg1	immunoglobulin G	immunoglobulin G			glycans	Cterm		immunoglobulin G			N-linked glycans on immunoglobulin G (IgG) have been associated with pathogenesis of diseases and the therapeutic functions of antibody-based drugs; however, low-abundance species are difficult to detect.
28931878	1	48	gly	glycans	99:105	arg1	IgG	IgG			glycans	Cterm		IgG			N-linked glycans on immunoglobulin G (IgG) have been associated with pathogenesis of diseases and the therapeutic functions of antibody-based drugs; however, low-abundance species are difficult to detect.
33560857	8	63	gly	N-glycosylation	1318:1332	arg2	certain N-glycosylation sites			certain N-glycosylation sites						sites	Moreover, notable differences were found on certain N-glycosylation sites between primary adenocarcinoma and metastatic tumor in regard to branching, bisection, sialylation, and fucosylation.
30982611	3	91	gly	glycosylation	640:652	arg2	functionally important N-linked glycosylation (Asn514) and glycosylphosphatidylinositol (GPI) anchor (Ser529) sites			functionally important N-linked glycosylation (Asn514) and glycosylphosphatidylinositol (GPI) anchor (Ser529) sites						sites	This variant, located in the final exon of GPC4, results in premature termination of the protein 51 amino acid residues prior to the stop codon, and in concomitant loss of functionally important N-linked glycosylation (Asn514) and glycosylphosphatidylinositol (GPI) anchor (Ser529) sites.
27728760	1	1	gly	glycoproteins	155:167	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In this report, we use a proteomic strategy to identify glycoproteins on the surface of exosomes derived from myeloid-derived suppressor cells (MDSCs), and then test if selected glycoproteins contribute to exosome-mediated chemotaxis and migration of MDSCs.
27728760	1	57	gly	glycoproteins	277:289	arg1	selected glycoproteins	selected glycoproteins				Fterm		glycoproteins			In this report, we use a proteomic strategy to identify glycoproteins on the surface of exosomes derived from myeloid-derived suppressor cells (MDSCs), and then test if selected glycoproteins contribute to exosome-mediated chemotaxis and migration of MDSCs.
31194940	1	39	gly	glycoprotein	156:167	arg1	Env	Env				PUBTATOR		Env)	155971		The fusion peptide (FP) of HIV-1 envelope glycoprotein (Env) is essential for mediating viral entry.
31194940	1	39	gly	glycoprotein	156:167	arg1	HIV-1 envelope glycoprotein	HIV-1 envelope glycoprotein				Fterm		glycoprotein			The fusion peptide (FP) of HIV-1 envelope glycoprotein (Env) is essential for mediating viral entry.
34401920	6	3	gly	glycosylation	833:845	arg2	at least one glycosylation site			at least one glycosylation site						site	82 N-linked glycoproteins with at least one glycosylation site were identified.
34401920	6	59	gly	glycoproteins	801:813	arg1	82 N-linked glycoproteins	82 N-linked glycoproteins				Fterm		glycoproteins			82 N-linked glycoproteins with at least one glycosylation site were identified.
30158294	16	37	gly	N-glycosylation	2481:2495	arg1	SERINC5	SERINC5				PUBTATOR		SERINC5	256987		Nonetheless, N-glycosylation per se is neither required for the ability of SERINC5 to inhibit HIV-1 infectivity nor for its sensitivity to antagonism by Nef.
30653653	1	8	gly	glycoprotein	181:192	arg1	glycoprotein				glycoprotein						The congenital disorders of glycosylation (CDG) are defects in glycoprotein and glycolipid glycan synthesis and attachment.
33045166	3	16	gly	glycopeptides	786:798	arg2	homogeneous N-linked glycopeptides			homogeneous N-linked glycopeptides						glycopeptides	This synthetic strategy demonstrates potential utility in accessing a wide range of homogeneous N-linked glycopeptides for the examination of their biological function.
32022078	7	9	gly	deglycosylated	951:964	arg1	the gas-phase deglycosylated peptide			the gas-phase deglycosylated peptide						peptide	Then, searching for Y0 (the gas-phase deglycosylated peptide) in HCD spectra, the potential glycoforms of these glycopeptides could be lined up.
32022078	7	19	gly	glycoforms	1005:1014	arg1	these glycopeptides			these glycopeptides						glycopeptides	Then, searching for Y0 (the gas-phase deglycosylated peptide) in HCD spectra, the potential glycoforms of these glycopeptides could be lined up.
32022078	7	42	gly	glycopeptides	1025:1037	arg2	these glycopeptides			these glycopeptides						glycopeptides	Then, searching for Y0 (the gas-phase deglycosylated peptide) in HCD spectra, the potential glycoforms of these glycopeptides could be lined up.
31837192	9	27	gly	glycoproteins	1674:1686	arg1	several plant-produced glycoproteins	several plant-produced glycoproteins				Fterm		glycoproteins			Transient BGAL1 down-regulation by RNA interference (RNAi) and BGAL1 depletion by genome editing drastically reduce β-galactosidase activity in N. benthamiana and increase the amounts of fully galactosylated complex N-glycans on several plant-produced glycoproteins.
32817340	7	3	gly	modified	1631:1638	arg3	the S-layer protein AND the same heptameric glycan	the S-layer protein			the same heptameric glycan	Fterm		protein			In addition, we confirmed that the S-layer protein is modified with the same heptameric glycan, suggesting a common N-glycosylation pathway.
28742148	4	17	gly	O-GlcNAc	866:873	arg1	proteins	proteins			O-GlcNAc	Fterm		proteins			O-GlcNAcylation consists in the addition of O-linked-N-acetylglucosamine (O-GlcNAc) on Serine or Threonine residues, a process controlled by two enzymes: O-GlcNAc transferase (OGT), which transfers O-GlcNAc on proteins, and O-GlcNAcase (OGA), which removes it.
28742148	4	78	gly	O-linked-N-acetylglucosamine	712:739	arg1	Threonine			Threonine	Threonine		AminoAcid			Threonine residues	O-GlcNAcylation consists in the addition of O-linked-N-acetylglucosamine (O-GlcNAc) on Serine or Threonine residues, a process controlled by two enzymes: O-GlcNAc transferase (OGT), which transfers O-GlcNAc on proteins, and O-GlcNAcase (OGA), which removes it.
28742148	4	78	gly	O-linked-N-acetylglucosamine	712:739	arg1	Serine			Serine	Serine		AminoAcid			Serine	O-GlcNAcylation consists in the addition of O-linked-N-acetylglucosamine (O-GlcNAc) on Serine or Threonine residues, a process controlled by two enzymes: O-GlcNAc transferase (OGT), which transfers O-GlcNAc on proteins, and O-GlcNAcase (OGA), which removes it.
30541852	13	111	gly	glycosylation	1972:1984	arg1	SLC19A1 and also other residues			SLC19A1 and also other residues						residues	We show that GLN infection is limited to mouse cells due to both a mutation in the mouse gene preventing the glycosylation of SLC19A1 and also other residues conserved within the rat but not in the hamster and human proteins.
28535613	7	13	gly	glycosylation	1268:1280	arg1	the purified rhBMP-4	the purified rhBMP-4				OGER		rhBMP-4	P12644		The N-terminal amino acid sequences and N-linked glycosylation of the purified rhBMP-4 were confirmed by N-terminal sequencing and de-N-glycosylation analysis, respectively.
31527085	3	4	gly	observed	367:374	arg2	sOGT AND multiple O-GlcNAc sites	sOGT			multiple O-GlcNAc sites	Cterm		sOGT	8473		Recently, multiple O-GlcNAc sites have been observed on short-form OGT (sOGT) and nucleocytoplasmic OGT (ncOGT), both of which locate in the nucleus and cytoplasm in cell.
31527085	3	4	gly	observed	367:374	arg2	ncOGT AND multiple O-GlcNAc sites	ncOGT			multiple O-GlcNAc sites	Cterm		ncOGT	8473		Recently, multiple O-GlcNAc sites have been observed on short-form OGT (sOGT) and nucleocytoplasmic OGT (ncOGT), both of which locate in the nucleus and cytoplasm in cell.
31527085	3	4	gly	observed	367:374	arg2	nucleocytoplasmic OGT AND multiple O-GlcNAc sites	OGT			multiple O-GlcNAc sites	PUBTATOR		OGT	8473		Recently, multiple O-GlcNAc sites have been observed on short-form OGT (sOGT) and nucleocytoplasmic OGT (ncOGT), both of which locate in the nucleus and cytoplasm in cell.
31000023	2	17	gly	glycoprotein	221:232	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			It was determined as a glycoprotein comprising 23.65% carbohydrate content with O-linked glycan and showed specific activity of 90.01 ± 1.2 U/mg against laminarin.
30712568	7	0	gly	N-glycosylation	1602:1616	arg2	348 unique N-glycosylation sites			348 unique N-glycosylation sites						sites	Finally, the Fe3O4@PMSA was applied for enriching N-linked glycopeptides from human serum, and 348 unique N-glycosylation sites and 419 glycopeptides from 158 glycoproteins were strictly identified from 1 μL human serum.
30712568	7	9	gly	glycopeptides	1555:1567	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	Finally, the Fe3O4@PMSA was applied for enriching N-linked glycopeptides from human serum, and 348 unique N-glycosylation sites and 419 glycopeptides from 158 glycoproteins were strictly identified from 1 μL human serum.
30712568	7	62	gly	glycoproteins	1655:1667	arg1	158 glycoproteins	158 glycoproteins				Fterm		glycoproteins			Finally, the Fe3O4@PMSA was applied for enriching N-linked glycopeptides from human serum, and 348 unique N-glycosylation sites and 419 glycopeptides from 158 glycoproteins were strictly identified from 1 μL human serum.
30712568	7	3	gly	glycopeptides	1632:1644	arg1	158 glycoproteins	glycoproteins		glycopeptides		Fterm		glycoproteins		glycopeptides	Finally, the Fe3O4@PMSA was applied for enriching N-linked glycopeptides from human serum, and 348 unique N-glycosylation sites and 419 glycopeptides from 158 glycoproteins were strictly identified from 1 μL human serum.
32281997	7	18	gly	glycoprotein	1188:1199	arg1	glycoprotein standards	glycoprotein standards				Fterm		glycoprotein			The approach was demonstrated using glycoprotein standards and further applied to analyze the glycosylation of the murine mammary gland NMuMG cell line.
33270232	6	15	gly	glycoprotein	665:676	arg1	Ub glycoprotein	Ub glycoprotein				Fterm		glycoprotein			Interestingly, immunized rabbits produced antibodies to both Ub glycoprotein and rUb; but antibodies against carbohydrates were immunodominant, rather than antibodies to the protein moiety of EhUbiquitin.
30865517	6	12	gly	modification	1238:1249	arg1	complex subunit proteins AND direct increased O-GlcNAc modification	complex subunit proteins			direct increased O-GlcNAc modification	Fterm		proteins			However, the observed bioenergetic changes appeared not to be due to direct increased O-GlcNAc modification of complex subunit proteins.
30865517	6	45	gly	proteins	1270:1277	arg1	direct increased O-GlcNAc modification	proteins			direct increased O-GlcNAc modification	Fterm		proteins			However, the observed bioenergetic changes appeared not to be due to direct increased O-GlcNAc modification of complex subunit proteins.
30250045	10	15	gly	sialylated	1467:1476	arg1	sialylated glycan modification				sialylated glycan modification						The specific detection of mesothelioma with SKM9-2 can thus be performed by the recognition of sialylated glycan modification in the specific region of HEG1.
29090410	6	52	gly	glycosylation	1007:1019	arg2	the putative N-linked glycosylation sites			the putative N-linked glycosylation sites						sites	In particular, the S32H, N33D, D34N, and S36G variations resulted in that HNhx lost all the putative N-linked glycosylation sites at amino acid positions 30, 32, 33, 34, and 35.
32938717	1	65	gly	glycoproteins	325:337	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Oligosaccharyltransferase (OST) is responsible for the first step in the N-linked glycosylation, transferring an oligosaccharide chain onto asparagine residues to create glycoproteins.
32965048	8	24	gly	N-glycosites	1741:1752	arg2	419 N-glycosites			419 N-glycosites						N-glycosites	The 2986 intact N-glycopeptide IDs corresponded to 754 putative N-glycan linkage structures on 419 N-glycosites of 450 peptide backbones from 327 intact N-glycoproteins.
32965048	8	36	gly	N-glycoproteins	1795:1809	arg1	327 intact N-glycoproteins	327 intact N-glycoproteins				Fterm		N-glycoproteins			The 2986 intact N-glycopeptide IDs corresponded to 754 putative N-glycan linkage structures on 419 N-glycosites of 450 peptide backbones from 327 intact N-glycoproteins.
32965048	8	123	gly	N-glycopeptide	1658:1671	arg2	The 2986 intact N-glycopeptide IDs			The 2986 intact N-glycopeptide IDs						N-glycopeptide	The 2986 intact N-glycopeptide IDs corresponded to 754 putative N-glycan linkage structures on 419 N-glycosites of 450 peptide backbones from 327 intact N-glycoproteins.
32965048	8	131	gly	structures	1723:1732	arg1	419 N-glycosites			419 N-glycosites	419 N-glycosites		Site			N-glycosites	The 2986 intact N-glycopeptide IDs corresponded to 754 putative N-glycan linkage structures on 419 N-glycosites of 450 peptide backbones from 327 intact N-glycoproteins.
29609090	8	26	gly	N-glycoproteins	1323:1337	arg1	important therapeutic mammalian N-glycoproteins	important therapeutic mammalian N-glycoproteins				Fterm		N-glycoproteins			The efficient glycosylation of natural short peptides by AaNGT showed its potential to modify important therapeutic mammalian N-glycoproteins.
29609090	8	38	gly	glycosylation	1211:1223	arg1	natural short peptides			natural short peptides						peptides	The efficient glycosylation of natural short peptides by AaNGT showed its potential to modify important therapeutic mammalian N-glycoproteins.
36303733	1	49	gly	protein	181:187	arg1	N-glycan structure	S) protein			N-glycan structure	OGER		S) protein	P04004		Background: The N-glycan structure and composition of the spike (S) protein of SARS-CoV-2 are pertinent to vaccine development and efficacy.
31604106	12	1	gly	O-glycosylation	2268:2282	arg1	recombinant hCG protein	recombinant hCG protein				PUBTATOR		hCG protein	93659		The results indicated that Galnt1 overexpression increased the recombinant hCG protein level by 1.57 times and improved the total galactosylation of intracellular proteins, O-glycosylation and the stability of recombinant hCG protein.
31671706	5	1	gly	glycosylation	1013:1025	arg2	each glycosylation site			each glycosylation site						site	Finally, it was clearly confirmed that N-linked glycans for each glycosylation site showed significantly different patterns in microheterogeneity, which may indicate certain functions for each glycosylation site in the protein.
31671706	5	25	gly	glycosylation	1141:1153	arg2	each glycosylation site			each glycosylation site						site	Finally, it was clearly confirmed that N-linked glycans for each glycosylation site showed significantly different patterns in microheterogeneity, which may indicate certain functions for each glycosylation site in the protein.
31511323	10	25	gly	glycosylation	1516:1528	arg2	a single intact glycosylation site			a single intact glycosylation site						site	Interestingly, β2 with a single intact glycosylation site was as effective as the WT in promoting NaV1.5 surface localization.
30368301	5	104	gly	heterogeneity	884:896	arg1	hCG	hCG				OGER		hCG			As expected, CGE led to a better resolution than SDS-PAGE and confirmed the large heterogeneity of hCG.
33629527	9	25	gly	fucosylated	1450:1460	arg1	Almost all N-glycan structures				Almost all N-glycan structures						Almost all N-glycan structures were fucosylated.
34404781	6	37	gly	erythropoietin	895:908	arg1	diverse N-glycan profiles	erythropoietin			diverse N-glycan profiles	PUBTATOR		erythropoietin	2056		Using GlyCompare, we study diverse N-glycan profiles from glycoengineered erythropoietin.
31337705	5	35	gly	sialylated	690:699	arg1	sialylated Lewis A				sialylated Lewis A						Herein, we found that the highest percentage of bovine sperm bound to the 3'-O-sulfated form of Lewis A (suLeA) trisaccharide and sialylated Lewis A and that fluoresceinated versions of each localized to receptors on the anterior head of the sperm.
33826885	5	48	gly	occupancy	698:706	arg2	the N160 site			the N160 site				Env	100616444	N160 site	Occupancy of the N133 site is increased by changing N133 to NxS, whereas occupancy of the N160 site is restored by reverting the nearby N156 sequon to NxS.
30016717	12	8	gly	containing	2238:2247	arg1	620 peptides AND one or more O-GlcNAc sites			620 peptides	one or more O-GlcNAc sites					peptides	A total of 620 peptides containing one or more O-GlcNAc sites were identified; interestingly, several of them belong to low expressed proteins, in particular proteins involved in signalling pathways.
34780171	4	16	gly	sialoglycopeptides	964:981	arg2	sialoglycopeptides			sialoglycopeptides						sialoglycopeptides	In a model study using lung cancer cells, early elution by a high percentage of acetonitrile prominently prefilters nonglycopeptides, facilitating high enrichment specificity for glycopeptides (92-96%) and sialoglycopeptides (77-89%) in the subsequent hydrophilic fractions.
34780171	4	49	gly	glycopeptides	937:949	arg2	glycopeptides			glycopeptides						glycopeptides	In a model study using lung cancer cells, early elution by a high percentage of acetonitrile prominently prefilters nonglycopeptides, facilitating high enrichment specificity for glycopeptides (92-96%) and sialoglycopeptides (77-89%) in the subsequent hydrophilic fractions.
34780171	4	75	gly	nonglycopeptides	874:889	arg2	nonglycopeptides			nonglycopeptides						nonglycopeptides	In a model study using lung cancer cells, early elution by a high percentage of acetonitrile prominently prefilters nonglycopeptides, facilitating high enrichment specificity for glycopeptides (92-96%) and sialoglycopeptides (77-89%) in the subsequent hydrophilic fractions.
30486487	5	11	gly	glycosylation	669:681	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	E2 protein was confirmed by Western blot assay, which showed that E2 protein possesses N-linked glycosylation sites.
33222001	7	13	gly	core-fucosylated	1091:1106	arg1	a complex core-fucosylated N-linked glycan				a complex core-fucosylated N-linked glycan						Furthermore, a complex core-fucosylated N-linked glycan was detected that could discriminate a primary tumour with and without LNM.
32168410	10	89	gly	Deglycosylation	1128:1142	arg1	the native protein	the native protein				Fterm		protein			Deglycosylation of the native protein was achieved by repeated digestion at elevated PNGase F concentration.
33826885	0	41	gly	trimers	53:59	arg1	glycan occupancy	trimers			glycan occupancy	Fterm		trimers			Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike.
34855283	6	44	gly	core-fucosylated	1040:1055	arg1	LacdiNAc-containing, tri-antennary, and core-fucosylated glycans				LacdiNAc-containing, tri-antennary, and core-fucosylated glycans						In addition to several bi-antennary and/or bisecting glycans that were commonly elevated in ICC and HCC, a number of LacdiNAc-containing, tri-antennary, and core-fucosylated glycans were uniquely increased in ICC.
34200965	3	13	gly	glycosylated	439:450	arg1	membrane proteins	membrane proteins				Fterm		proteins			In particular, membrane proteins are frequently highly glycosylated, which is both linked to physiological processes and of high relevance in various disease mechanisms.
34809984	2	58	gly	glycosylation	215:227	arg1	these proteins	these proteins				Fterm		proteins			The glycosylation profile of these proteins is a key quality parameter as it can affect their safety, efficacy, and stability.
31837192	4	54	gly	glycoproteins	740:752	arg1	many plant-derived glycoproteins	many plant-derived glycoproteins				Fterm		glycoproteins			Despite significant efforts to optimize the expression of β1,4-galactosyltransferase, many plant-derived glycoproteins still exhibit incomplete processed N-glycans with heterogeneous terminal galactosylation.
33525794	4	62	gly	glycoprotein	1110:1121	arg1	high-value glycoprotein targets	high-value glycoprotein targets				Fterm		glycoprotein			Here, we highlight recent advances in the deployment of metabolic engineering tools and strategies to develop microbial cell glyco-factories for the production of high-value glycoprotein targets with applications in research and medicine.
30563903	6	8	gly	deglycosylation	1163:1177	arg1	fXII	fXII				OGER		fXII	P00748		At both sites, cleavage occurs between proline and an O-linked glycosylated threonine, and deglycosylation of fXII prevents cleavage by CpaA.
31722217	0	39	gly	Glycosylation	0:12	arg1	Specific Notch EGF Repeats				Specific Notch EGF Repeats						Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila.
35140700	11	23	gly	sialylation	1710:1720	arg1	IgG	IgG				Cterm		IgG			Specifically, the results of lectin microarray showed the galactose level of IgG was increased by IFN-γ stimulation (p<0.05), and the sialylation of IgG was increased by IL-21 and IL-17A (p<0.05).
35140700	11	48	gly	IgG	1725:1727	arg1	the sialylation	IgG			the sialylation	Cterm		IgG			Specifically, the results of lectin microarray showed the galactose level of IgG was increased by IFN-γ stimulation (p<0.05), and the sialylation of IgG was increased by IL-21 and IL-17A (p<0.05).
35140700	11	49	gly	IgG	1653:1655	arg1	the galactose level	IgG			the galactose level	Cterm		IgG			Specifically, the results of lectin microarray showed the galactose level of IgG was increased by IFN-γ stimulation (p<0.05), and the sialylation of IgG was increased by IL-21 and IL-17A (p<0.05).
30506260	3	12	gly	glycosylate	633:643	arg1	proteins	proteins				Fterm		proteins			Chlamydomonas reinhardtii chloroplast is an attractive platform for expressing malaria antigens because it is capable of folding complex proteins, including those requiring disulfide bond formation, while lack the ability to glycosylate proteins; a valuable quality of any malaria protein expression system, since the Plasmodium parasite lacks N-linked glycosylation machinery.
31178836	1	79	gly	glycoprotein	170:181	arg1	env	env				PUBTATOR		env	155971		Exploring the characteristics of the HIV-1 envelope glycoprotein (env) gene in a natural HIV-1 infected individual, with broadly neutralizing activity, may provide insight into the generation of such broadly neutralizing antibodies and initiate the design of an appropriate immunogen.
31178836	1	79	gly	glycoprotein	170:181	arg1	HIV-1 envelope glycoprotein	HIV-1 envelope glycoprotein				PUBTATOR		HIV-1 envelope glycoprotein	155971		Exploring the characteristics of the HIV-1 envelope glycoprotein (env) gene in a natural HIV-1 infected individual, with broadly neutralizing activity, may provide insight into the generation of such broadly neutralizing antibodies and initiate the design of an appropriate immunogen.
32719555	2	5	gly	O-glycoproteins	550:564	arg1	different human O-glycoproteins	different human O-glycoproteins				Fterm		O-glycoproteins			Here, we have developed a set of orthogonal pathways for eukaryotic O-linked protein glycosylation in Escherichia coli that installed the cancer-associated mucin-type glycans Tn, T, sialyl-Tn and sialyl-T onto serine residues in acceptor motifs derived from different human O-glycoproteins.
31164895	8	24	gly	fucosylation	1320:1331	arg1	proteins	proteins				Fterm		proteins			We show that the P. tricornutum transporter is able to rescue the fucosylation of proteins in this CHO-gmt5 mutant cell line, thus demonstrating the functional activity of the diatom transporter and its appropriate Golgi localization.
31164895	8	69	gly	proteins	1336:1343	arg1	the fucosylation	proteins			the fucosylation	Fterm		proteins			We show that the P. tricornutum transporter is able to rescue the fucosylation of proteins in this CHO-gmt5 mutant cell line, thus demonstrating the functional activity of the diatom transporter and its appropriate Golgi localization.
29237830	6	48	gly	glycosylated	915:926	arg1	differentially glycosylated DG	differentially glycosylated DG				Cterm		DG	1605		Here, we examine LASV receptor candidates in primary human cells and found coexpression of Axl with differentially glycosylated DG.
34401920	7	69	gly	glycosylation	878:890	arg2	the sequon			the sequon						sequon, Asn	N-Linked glycosylation occurred at the sequon, Asn-X-Ser/Thr, and the proportions of Ser and Thr glycosylation at the hydroxy position were found 39.6% and 60.3%, respectively.
30755292	4	16	gly	N-glycosylation	808:822	arg2	potential N-glycosylation sites			potential N-glycosylation sites						sites	METHODS We determined the HA sequences of A(H3N2) viruses detected in Yokohama, Japan during the 2016/17 and 2017/18 influenza seasons to identify amino acid substitutions and the loss or gain of potential N-glycosylation sites in HA, both of which potentially affect the antigenicity of HA.
27704558	2	56	gly	glycoprotein	397:408	arg1	the small integrin binding ligand N-linked glycoprotein (SIBLING) family	the small integrin binding ligand N-linked glycoprotein (SIBLING) family				Fterm		glycoprotein			Bone sialoprotein (BSP) is with osteopontin (OPN) a member of the small integrin binding ligand N-linked glycoprotein (SIBLING) family, involved in bone formation, hematopoiesis and angiogenesis.
33116178	8	67	gly	structures	1309:1318	arg1	recombinant proteins	proteins			structures	Fterm		proteins			The functionally characterized SmFucTs can also be applied to synthesize complex N-glycan structures on recombinant proteins to study their contribution to immunomodulation.
29460617	1	18	gly	glycosylation	111:123	arg1	nucleocytoplasmic proteins	nucleocytoplasmic proteins				Fterm		proteins			The glycosylation of nucleocytoplasmic proteins with O-linked N-acetylglucosamine residues (O-GlcNAc) is conserved among metazoans and is particularly abundant within brain.
32432921	9	16	gly	attached	1380:1387	arg1	the N-glycosylation site AND The glycan			the N-glycosylation site	The glycan					site	The glycan attached to the N-glycosylation site was found to be composed of fucose, galactose, sialic acid, and N-acetylglucosamine.
32432921	9	31	gly	N-glycosylation	1396:1410	arg2	the N-glycosylation site			the N-glycosylation site						site	The glycan attached to the N-glycosylation site was found to be composed of fucose, galactose, sialic acid, and N-acetylglucosamine.
32553552	4	58	gly	N-glycoproteins	569:583	arg1	the N-glycoproteins	the N-glycoproteins				Fterm		N-glycoproteins			The present study used mass spectrometry-based approaches to characterize the N-glycoproteins present in the midgut cell microvilli of Spodoptera frugiperda.
34857845	8	46	gly	used	1568:1571	arg2	tri- and tetra-antennary core fucosylated glycopeptides			tri- and tetra-antennary core fucosylated glycopeptides						glycopeptides	Further studies are needed to verify whether tri- and tetra-antennary core fucosylated glycopeptides could be used as markers of liver disease progression.
34857845	8	48	gly	fucosylated	1533:1543	arg1	tri- and tetra-antennary core fucosylated glycopeptides			tri- and tetra-antennary core fucosylated glycopeptides						glycopeptides	Further studies are needed to verify whether tri- and tetra-antennary core fucosylated glycopeptides could be used as markers of liver disease progression.
34857845	8	102	gly	glycopeptides	1545:1557	arg2	tri- and tetra-antennary core fucosylated glycopeptides			tri- and tetra-antennary core fucosylated glycopeptides						glycopeptides	Further studies are needed to verify whether tri- and tetra-antennary core fucosylated glycopeptides could be used as markers of liver disease progression.
32001344	7	25	gly	glycosylation	1157:1169	arg2	the active N-linked glycosylation sites			the active N-linked glycosylation sites						sites	By site-directed mutagenesis, we identified amino acids 57 and 330 as the active N-linked glycosylation sites on V3 when expressed in this cell type.
32693290	3	44	gly	peptide	660:666	arg1	Thr163			Thr159, Thr163 and Thr169				factor IX	P00740	Thr159, Thr163 and Thr169	Three O-linked glycan sites were identified in the activation peptide (Thr159, Thr163 and Thr169), where Thr163 has not been reported previously.
32693290	3	44	gly	peptide	660:666	arg1	Thr159			Thr159, Thr163 and Thr169				factor IX	P00740	Thr159, Thr163 and Thr169	Three O-linked glycan sites were identified in the activation peptide (Thr159, Thr163 and Thr169), where Thr163 has not been reported previously.
32693290	3	44	gly	peptide	660:666	arg1	Thr159			Thr159, Thr163 and Thr169				factor IX	P00740	Thr159, Thr163 and Thr169	Three O-linked glycan sites were identified in the activation peptide (Thr159, Thr163 and Thr169), where Thr163 has not been reported previously.
31164864	5	26	gly	proteins	786:793	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins			O-GlcNAcylation of proteins is controlled by two enzymes: the O-GlcNAc transferase (OGT), which transfers a monosaccharide to serine/threonine residues on target proteins, and the O-GlcNAcase (OGA), which removes it.
31164895	10	36	gly	-fucosylation	1759:1771	arg1	the diatom endogenous glycoproteins	the diatom endogenous glycoproteins				Fterm		glycoproteins			Our findings show that overexpression of the FuT54599 leads to a significant increase of the α(1,3)-fucosylation of the diatom endogenous glycoproteins.
31164895	10	81	gly	glycoproteins	1798:1810	arg1	the diatom endogenous glycoproteins	glycoproteins			the α(1,3)-fucosylation	Fterm		glycoproteins			Our findings show that overexpression of the FuT54599 leads to a significant increase of the α(1,3)-fucosylation of the diatom endogenous glycoproteins.
33347638	6	106	gly	glycoprotein	1053:1064	arg1	the newly identified glycans	glycoprotein			the newly identified glycans	Fterm		glycoprotein			Finally, we constructed a new assay to quantify a candidate glycoprotein with the newly identified glycans.
32699088	0	36	gly	Sites	16:20	arg1	the Influenza A Virus Neuraminidase Head Domain	Domain			Sites	Fterm		Domain			N-Linked Glycan Sites on the Influenza A Virus Neuraminidase Head Domain Are Required for Efficient Viral Incorporation and Replication.
32168410	4	1	gly	Asparagine-linked	574:590	arg1	Asparagine-linked carbohydrates			Asparagine	Asparagine-linked carbohydrates					Asparagine	METHODS Asparagine-linked carbohydrates were released from human FXIIII-B by PNGase F digestion.
32461612	2	17	gly	glycoprotein	368:379	arg1	the spike (S) glycoprotein	the spike (S) glycoprotein				Fterm		glycoprotein			Vaccine development focuses on the principal target of the neutralizing humoral immune response, the spike (S) glycoprotein.
30445613	1	27	gly	glycosylation	214:226	arg1	proteins	proteins				Fterm		proteins			N-linked glycosylation of proteins is the most common post-translational modification of proteins.
28031460	6	43	gly	α2,6-sialylated	1017:1031	arg1	the target α2,6-sialylated proteins	the target α2,6-sialylated proteins				Fterm		proteins			Using proteomic analysis, integrin-β1 was identified as one of the target α2,6-sialylated proteins in adipose tissues, and phosphorylation of its downstream molecule focal adhesion kinase was found to be decreased after HFD feeding.
34647616	5	21	gly	sialylated	1162:1171	arg1	heavily sialylated F241A antibody N-glycans				heavily sialylated F241A antibody N-glycans						Furthermore, overexpression of recombinant human α-2,6-sialyltransferase resulted in a predominance of α-2,6-sialylation rather than α-2,3-sialylation for both WT and heavily sialylated F241A antibody N-glycans.
33177111	6	15	gly	glycosylation	789:801	arg2	one N-linked glycosylation site			one N-linked glycosylation site						site	CD55 had one N-linked glycosylation site in addition to a Ser/Thr-rich domain, which was expected to be heavily O-glycosylated.
33177111	6	65	gly	O-glycosylated	879:892	arg1	a Ser/Thr-rich domain			a Ser/Thr-rich domain						domain	CD55 had one N-linked glycosylation site in addition to a Ser/Thr-rich domain, which was expected to be heavily O-glycosylated.
28687873	3	17	gly	sialylated	544:553	arg1	sialylated glycan receptor analogues				sialylated glycan receptor analogues						METHODS Surface plasmon resonance analysis and competitive inhibition with sialylated glycan receptor analogues were used to determine the affinity and the preference of sialic acid receptor.
32258897	2	60	gly	heterogeneity	335:347	arg1	carbohydrate moieties				carbohydrate moieties						Herein, we characterized N-glycosylation heterogeneity of R27T, which includes both N-glycan site occupancy heterogeneity (macro-heterogeneity) and complexity of carbohydrate moieties (micro-heterogeneity).
32258897	1	13	gly	glycosylation	182:194	arg2	an additional glycosylation site			an additional glycosylation site						site	We previously developed a biobetter version of rhIFN-β (R27T) that possesses an additional glycosylation site compared with rhIFN-β 1a.
32384234	5	71	gly	glycosylated	1272:1283	arg1	a multiply glycosylated peptide			a multiply glycosylated peptide						peptide	Importantly, direct elimination of one or more O-glycans under negative-mode MS2 affords an easy way to discover additional O-glycosylations on a multiply glycosylated peptide by virtue of enumerating the dehydration scars imprinted on the O-glycosylated sites.
32384234	5	77	gly	O-glycosylated	1357:1370	arg1	the O-glycosylated sites			the O-glycosylated sites						sites	Importantly, direct elimination of one or more O-glycans under negative-mode MS2 affords an easy way to discover additional O-glycosylations on a multiply glycosylated peptide by virtue of enumerating the dehydration scars imprinted on the O-glycosylated sites.
29899104	6	44	gly	glycosylation	942:954	arg1	the E protein	the E protein				Fterm		protein			The mutation S156P in the domain I resulted in disruption of N-linked glycosylation at amino acid 154 of the E protein and changed the conformation of "150 loop" of the E protein, which reduced virus replication in lungs and abrogated transmission in ducks.
31450586	7	24	gly	glycopeptides	958:970	arg2	1299 unique N-linked glycopeptides			1299 unique N-linked glycopeptides						glycopeptides	We identified 1299 unique N-linked glycopeptides corresponding to 460 proteins.
29384693	0	76	gly	glycosylation	18:30	arg1	cyclooxygenase-2	cyclooxygenase-2				PUBTATOR		cyclooxygenase-2	5743		Palmitate induces glycosylation of cyclooxygenase-2 in primary human vascular smooth muscle cells.
30913278	7	15	gly	pro-drug	1224:1231	arg1	GlcNAc-6-acetate			pro	GlcNAc-6-acetate					pro	Here we report that GlcNAc-6-acetate is a superior pro-drug form of GlcNAc.
29454068	15	75	gly	receptor	2000:2007	arg1	O-GlcNAcylation	receptor for activated C-kinase 1			O-GlcNAcylation	PUBTATOR		receptor for activated C-kinase 1	10399		LAY SUMMARY: O-GlcNAcylation of ribosomal receptor for activated C-kinase 1 at the amino acid serine122 promotes its stability, ribosome localization and interaction with the protein kinase, PKCβII, thus driving the translation of oncogenes and tumorigenesis of hepatocellular carcinoma.
29454068	15	87	gly	serine122	2052:2060	arg1	O-GlcNAcylation	receptor for activated C-kinase 1		serine122	O-GlcNAcylation			receptor for activated C-kinase 1	10399	serine122	LAY SUMMARY: O-GlcNAcylation of ribosomal receptor for activated C-kinase 1 at the amino acid serine122 promotes its stability, ribosome localization and interaction with the protein kinase, PKCβII, thus driving the translation of oncogenes and tumorigenesis of hepatocellular carcinoma.
33578083	3	22	gly	glycans	858:864	arg1	the initial glycan database	database			glycans	Fterm		database			Even at this level, however, glycopeptide analysis poses challenges: finding glycopeptide spectra when they are a tiny fraction of the total spectra; assigning spectra with unanticipated glycans, not in the initial glycan database; and finding, scoring, and labeling diagnostic peaks in tandem mass spectra.
33578083	3	78	gly	glycopeptide	748:759	arg2	finding glycopeptide spectra			finding glycopeptide spectra						glycopeptide	Even at this level, however, glycopeptide analysis poses challenges: finding glycopeptide spectra when they are a tiny fraction of the total spectra; assigning spectra with unanticipated glycans, not in the initial glycan database; and finding, scoring, and labeling diagnostic peaks in tandem mass spectra.
33578083	3	87	gly	glycopeptide	700:711	arg2	glycopeptide analysis			glycopeptide analysis						glycopeptide	Even at this level, however, glycopeptide analysis poses challenges: finding glycopeptide spectra when they are a tiny fraction of the total spectra; assigning spectra with unanticipated glycans, not in the initial glycan database; and finding, scoring, and labeling diagnostic peaks in tandem mass spectra.
31019513	9	56	gly	glycosylation	1549:1561	arg1	CD22	CD22				PUBTATOR		CD22	933		We used dual-color super-resolution imaging to investigate the impact of altered glycosylation of CD22 on the nanoscale organization of CD22 and its association with BCR.
28302723	11	2	gly	proteins	2084:2091	arg1	the O-GlcNAc post-translational modification	proteins			the O-GlcNAc post-translational modification	Fterm		proteins			Thus, a single amino acid substitution in the regulatory domain (the tetratricopeptide repeat domain) of OGT, which catalyzes the O-GlcNAc post-translational modification of nuclear and cytosolic proteins, appears causal for XLID.
28302723	11	30	gly	OGT	1993:1995	arg1	the tetratricopeptide repeat domain	OGT			the tetratricopeptide repeat domain	PUBTATOR		OGT	8473		Thus, a single amino acid substitution in the regulatory domain (the tetratricopeptide repeat domain) of OGT, which catalyzes the O-GlcNAc post-translational modification of nuclear and cytosolic proteins, appears causal for XLID.
31800099	5	14	gly	hypoglycosylated	859:874	arg1	Plasma CETP	Plasma CETP				PUBTATOR		Plasma CETP	1071		Plasma CETP was hypoglycosylated and less active in B4GALT1-CDG patients compared to matched controls.
31019513	7	42	gly	residues	1322:1329	arg1	CD22	CD22			residues	PUBTATOR		CD22	933		To this end, we mutated five out of the six N-linked glycosylation residues on CD22 localized closest to the sialic acid binding site.
32898699	16	4	gly	N-glycosylation	2308:2322	arg1	proteins	proteins				Fterm		proteins			Our report deals with identification of differences in N-glycosylation of proteins in tissue and serum samples from the individuals showing sensitivity or resistance to platinum/taxane-based chemotherapy.
30422384	3	10	gly	glycosylated	625:636	arg1	recombinant glycosylated pro-ATSf-9			recombinant glycosylated pro-ATSf-9						pro	We chemically synthesized aglycosyl pro-AT and pro-CT and purified recombinant glycosylated pro-ATSf-9 .
32326134	3	50	gly	O-glycosylation	487:501	arg2	O-glycosylation sites			O-glycosylation sites						sites	This study investigates the structural and quantitative characteristics of O-glycans and identifies O-glycosylation sites in BSM using liquid chromatography-tandem mass spectrometry.
29782851	3	31	gly	second	484:489	arg1	a region			a region						region	Cbln1, 2 and 4 harbor two N-linked glycosylation sites, one at the N-terminus is in a region implicated in Nrxn binding and the second is in the C1q domain, a region involved in Grid2 binding.
29782851	3	31	gly	second	484:489	arg1	the C1q domain			the C1q domain						domain	Cbln1, 2 and 4 harbor two N-linked glycosylation sites, one at the N-terminus is in a region implicated in Nrxn binding and the second is in the C1q domain, a region involved in Grid2 binding.
29782851	3	47	gly	N-terminus	423:432	arg1	a region			a region						region	Cbln1, 2 and 4 harbor two N-linked glycosylation sites, one at the N-terminus is in a region implicated in Nrxn binding and the second is in the C1q domain, a region involved in Grid2 binding.
29782851	3	57	gly	glycosylation	391:403	arg2	two N-linked glycosylation sites			two N-linked glycosylation sites						sites	Cbln1, 2 and 4 harbor two N-linked glycosylation sites, one at the N-terminus is in a region implicated in Nrxn binding and the second is in the C1q domain, a region involved in Grid2 binding.
31256377	4	3	gly	attached	442:449	arg1	Hyl AND a single galactose unit	Hyl			a single galactose unit	OGER		Hyl	P42679		Then, to the 5-hydroxyl group of Hyl, a single galactose unit can be attached to form galactosyl-Hyl (Gal-Hyl) and further glucose can be added to Gal-Hyl to form glucosylgalactosyl-Hyl (GlcGal-Hyl).
31281932	0	34	gly	proteins	69:76	arg1	sialylation	proteins			sialylation	Fterm		proteins			Comparison of α2,6-sialyltransferases for sialylation of therapeutic proteins.
31281932	0	46	gly	sialylation	42:52	arg1	therapeutic proteins	therapeutic proteins				Fterm		proteins			Comparison of α2,6-sialyltransferases for sialylation of therapeutic proteins.
34401920	3	15	gly	glycoproteins	559:571	arg1	putative glycoproteins	putative glycoproteins				Fterm		glycoproteins			Results of SDS-PAGE and periodic acid-Schiff staining revealed that most serum proteins with high abundance were putative glycoproteins.
31067000	8	10	gly	domain	1268:1273	arg1	V domain glycans				V domain glycans						RESULTS In both data sets, FDR-derived IgG ACPA displayed markedly lower levels of V domain glycans (<50%) compared to IgG ACPA from RA patients.
33664361	6	58	gly	glycopeptide	966:977	arg2	the former glycopeptide			the former glycopeptide						glycopeptide	Nevertheless, N-glycosylation could be proved by enzymatic de-N-glycosylation with PNGase F, resulting in a strong MS-signal of the former glycopeptide with deamidated asparagine at the potential N-glycosylation site N444.
33664361	6	34	gly	N-glycosylation	1023:1037	arg2	N444			site N444				ZIKV		site N444	Nevertheless, N-glycosylation could be proved by enzymatic de-N-glycosylation with PNGase F, resulting in a strong MS-signal of the former glycopeptide with deamidated asparagine at the potential N-glycosylation site N444.
31308178	2	5	gly	receptor	468:475	arg1	sialic acid-capped N-glycans	vascular endothelial growth factor receptor 2			sialic acid-capped N-glycans	PUBTATOR		vascular endothelial growth factor receptor 2	3791		By altering the N-glycosylation machinery in the endoplasmic reticulum and Golgi, proinflammatory cytokines promote the modification of endothelial glycoproteins such as vascular endothelial growth factor receptor 2 (VEGFR2) with sialic acid-capped N-glycans.
31308178	2	40	gly	glycoproteins	411:423	arg1	vascular endothelial growth factor receptor 2	vascular endothelial growth factor receptor 2				PUBTATOR		vascular endothelial growth factor receptor 2	3791		By altering the N-glycosylation machinery in the endoplasmic reticulum and Golgi, proinflammatory cytokines promote the modification of endothelial glycoproteins such as vascular endothelial growth factor receptor 2 (VEGFR2) with sialic acid-capped N-glycans.
31308178	2	40	gly	glycoproteins	411:423	arg1	endothelial glycoproteins	endothelial glycoproteins				Fterm		glycoproteins			By altering the N-glycosylation machinery in the endoplasmic reticulum and Golgi, proinflammatory cytokines promote the modification of endothelial glycoproteins such as vascular endothelial growth factor receptor 2 (VEGFR2) with sialic acid-capped N-glycans.
33806155	8	5	gly	O-glycosylation	1393:1407	arg1	subunit vaccine design	subunit vaccine design				Fterm		subunit			This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.
29615517	4	1	gly	glycosylation	720:732	arg1	NgR1	NgR1				PUBTATOR		NgR1	65079		Endogenous and overexpressed ORL1 coimmunoprecipitated with immature NgR1 protein, and ORL1 enhanced the O-linked glycosylation and surface expression of NgR1 in HEK293T and Neuro2A cells and primary neurons.
32842538	3	35	gly	glycosylation	369:381	arg2	a single N-linked glycosylation site			a single N-linked glycosylation site						site	The prM protein of all ZIKV strains contains a single N-linked glycosylation site, while not all strains contain an N-linked site in the E protein.
32965048	10	28	gly	N-glycosites	1999:2010	arg2	106 new N-glycosites			106 new N-glycosites						N-glycosites	CONCLUSIONS We found 106 new N-glycosites not annotated in the current UniProt database.
30962950	1	0	gly	glycoproteins	165:177	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The goal of the present study was to identify glycoproteins associated with the postoperative relapse of hepatocellular carcinoma (HCC) and to investigate their potential role in HCC metastasis.
31480247	5	19	gly	glycosylation	948:960	arg2	the glycosylation motif			the glycosylation motif						motif	Using site-directed mutagenesis to obtain mutated versions of the two PSIs, with and without the glycosylation motif, we observed that altering the glycosylation pattern interferes with the trafficking of the protein as the non-glycosylated PSI-B, unlike its native glycosylated form, is able to bypass ER-to-Golgi blockage and accumulate in the vacuole.
33806155	2	87	gly	O-glycosites	435:446	arg2	a few O-glycosites			a few O-glycosites						O-glycosites	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.
31266872	1	49	gly	glycoproteins	443:455	arg1	phylogenetically and antigenically distinct envelope glycoproteins	phylogenetically and antigenically distinct envelope glycoproteins				Fterm		glycoproteins			As a consequence of their independent evolutionary origins in apes and Old World monkeys, human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency viruses of the SIVsmm/mac lineage express phylogenetically and antigenically distinct envelope glycoproteins.
33030205	1	9	gly	glycosylation	103:115	arg1	proteins	proteins				Fterm		proteins			The glycosylation of proteins is typically considered as a stabilizing modification, including resistance to proteolysis.
29769320	3	8	gly	OGT	546:548	arg1	the tetratricopeptide (TPR) repeats	OGT			the tetratricopeptide (TPR) repeats	PUBTATOR		OGT	8473		Three missense mutations in the tetratricopeptide (TPR) repeats of OGT have recently been reported to cause X-linked intellectual disability (XLID).
29769320	3	8	gly	OGT	546:548	arg1	TPR	OGT			TPR	PUBTATOR		OGT	8473		Three missense mutations in the tetratricopeptide (TPR) repeats of OGT have recently been reported to cause X-linked intellectual disability (XLID).
31333671	5	35	gly	glycosylation	729:741	arg2	N-linked glycosylation motifs			N-linked glycosylation motifs						motifs	Sequences were analyzed for clonal relationships, degree of somatic hypermutation, IGHV gene usage, evidence of antigenic selection, and N-linked glycosylation motifs.
29569918	3	9	gly	sialylated	602:611	arg1	unsialylated and sialylated glycans				unsialylated and sialylated glycans						Combining these two methods, we developed tandem labeling and double labeling strategies that permit the detection of unsialylated and sialylated glycans or the detection of O- and N-linked glycans on the same tissue section, respectively.
29569918	3	10	gly	unsialylated	585:596	arg1	unsialylated and sialylated glycans				unsialylated and sialylated glycans						Combining these two methods, we developed tandem labeling and double labeling strategies that permit the detection of unsialylated and sialylated glycans or the detection of O- and N-linked glycans on the same tissue section, respectively.
33167210	9	60	gly	glycopeptides	1489:1501	arg2	PSA glycopeptides			PSA glycopeptides						glycopeptides	Next to seminal plasma, the method is also expected to be of use for studying PSA glycopeptides derived from other biofluids and/or in other disease contexts.
30657688	4	40	gly	moiety	636:641	arg1	peptides			peptides	peptides		Site			peptides	In this method, the O-GlcNAc moiety on peptides was labeled with UDP-GalNAz followed by copper-free azide-alkyne cycloaddition with a multifunctional reagent bearing a terminal cyclooctyne, a disulfide bridge, and a biotin handle.
29305779	5	55	gly	moiety	1151:1156	arg1	LW-1	LW-1			moiety	PUBTATOR		LW-1	100506164		Advanced NMR techniques (1H-NMR, 13C-NMR, 1H-13C HSQC, 1H-1H TOCSY, 1H-13C HMBC) together with LC-mass spectrometry (MS) revealed a loss of 176 amu (atomic mass unit) unequivocally point to the presence of a glucuronic acid moiety in LW-1.
31186110	0	43	gly	glycosylated	6:17	arg1	Novel glycosylated human interferon alpha 2b	Novel glycosylated human interferon alpha 2b				PUBTATOR		interferon alpha 2b	3440		Novel glycosylated human interferon alpha 2b expressed in glycoengineered Pichia pastoris and its biological activity: N-linked glycoengineering approach.
34278967	4	79	gly	glycosylation	1020:1032	arg2	N-/O-linked glycosylation site			N-/O-linked glycosylation site						site	The Lens culinaris-derived lentil lectin which specifically bind to oligomannose-type glycans and GlcNAc at the non-reducing end terminus showed most potent and broad antiviral activity against a panel of mutant strains and variants, including the artificial mutants at N-/O-linked glycosylation site, natural existed amino acid mutants, as well as the epidemic variants B.1.1.7, B.1.351, and P.1.
32699088	8	129	gly	glycosylation	1404:1416	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Together, these results indicate that in addition to antigenicity, N-linked glycosylation sites can alter NA enzymatic stability and the NA amount in virions.IMPORTANCE N-linked glycans are transferred to secretory proteins upon entry into the endoplasmic reticulum lumen.
32692906	0	8	gly	O-glycans	0:8	arg1	death receptors	receptors			O-glycans	Fterm		receptors			O-glycans on death receptors in cells modulate their sensitivity to TRAIL-induced apoptosis through affecting on their stability and oligomerization.
30712568	1	40	gly	glycopeptides	321:333	arg2	glycopeptides			glycopeptides						glycopeptides	Hydrophilic interaction chromatography (HILIC) utilizing zwitterion-modified material as solid phase has attracted extensive attention for selective enrichment of glycopeptides.
29321565	8	20	gly	glycoproteins	1660:1672	arg1	natural glycoproteins	natural glycoproteins				Fterm		glycoproteins			Recombinant ORF1188, Beauveria and Cordyceps ENGases released the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B, a result that not only confirmed the substrate specificity of these novel ENGases but also suggested that natural glycoproteins could be their substrates.
29321565	8	36	gly	B	1543:1543	arg1	the high-mannose-containing oligosaccharides residues	RNase B			the high-mannose-containing oligosaccharides residues	OGER		RNase B	P07998		Recombinant ORF1188, Beauveria and Cordyceps ENGases released the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B, a result that not only confirmed the substrate specificity of these novel ENGases but also suggested that natural glycoproteins could be their substrates.
29933399	8	67	gly	glycosylation	1642:1654	arg2	three conserved N-linked glycosylation sites			three conserved N-linked glycosylation sites						sites	Here, in addition to serological analysis, we employed high-throughput BCR sequence analysis from the periphery, lymph nodes and bone marrow, as well as B cell- and antibody-isolation and characterization methods, to compare in great detail the B cell and antibody responses elicited in non-human primates by two forms of the clade C HIV Env 426c: one representing the full length extracellular portion of Env while the other lacking the variable domains 1, 2 and 3 and three conserved N-linked glycosylation sites.
29339411	3	21	gly	glycoprotein	598:609	arg1	a Selenomonas glycoprotein	a Selenomonas glycoprotein				Fterm		glycoprotein			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
29339411	3	66	gly	O-glycosylated	671:684	arg1	a major cellular protein	a major cellular protein				Fterm		protein			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
29339411	3	66	gly	O-glycosylated	671:684	arg1	a diversely and heavily O-glycosylated flagellin C9LY14	a diversely and heavily O-glycosylated flagellin C9LY14				Cterm		C9LY14			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
29339411	3	82	gly	carries	738:744	arg1	a major cellular protein AND hitherto undescribed rhamnose-	a major cellular protein			hitherto undescribed rhamnose-	Fterm		protein			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
29339411	3	82	gly	carries	738:744	arg1	a major cellular protein AND N-acetylglucosamine linked O-glycans	a major cellular protein			N-acetylglucosamine linked O-glycans	Fterm		protein			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
29339411	3	82	gly	carries	738:744	arg1	a diversely and heavily O-glycosylated flagellin C9LY14 AND hitherto undescribed rhamnose-	a diversely and heavily O-glycosylated flagellin C9LY14			hitherto undescribed rhamnose-	Cterm		C9LY14			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
29339411	3	82	gly	carries	738:744	arg1	a diversely and heavily O-glycosylated flagellin C9LY14 AND N-acetylglucosamine linked O-glycans	a diversely and heavily O-glycosylated flagellin C9LY14			N-acetylglucosamine linked O-glycans	Cterm		C9LY14			Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides.
31336133	2	1	gly	glycosylated	300:311	arg1	glycosylated peptides			glycosylated peptides						peptides	The genetic fusion to natural or synthetic glycosylated peptides constitutes a promising strategy since it conserves the protein sequence and results in the improvement of the pharmacokinetic properties.
31336133	2	79	gly	natural	279:285	arg1	glycosylated peptides			glycosylated peptides						peptides	The genetic fusion to natural or synthetic glycosylated peptides constitutes a promising strategy since it conserves the protein sequence and results in the improvement of the pharmacokinetic properties.
31306420	0	50	gly	glycosylation	9:21	arg1	the West Nile virus envelope protein	the West Nile virus envelope protein				Fterm		protein			N-linked glycosylation of the West Nile virus envelope protein is not a requisite for avian virulence or vector competence.
29902539	4	11	gly	glycoprotein	701:712	arg1	a large dimeric glycoprotein	a large dimeric glycoprotein				Fterm		glycoprotein			This cardiotoxic hemolytic cytolysin is a large dimeric glycoprotein (subunits of ≈65 kDa) with pore-forming ability.
29902539	4	11	gly	glycoprotein	701:712	arg1	This cardiotoxic hemolytic cytolysin	This cardiotoxic hemolytic cytolysin				Fterm		cytolysin			This cardiotoxic hemolytic cytolysin is a large dimeric glycoprotein (subunits of ≈65 kDa) with pore-forming ability.
30368301	3	96	gly	glycosylation	438:450	arg2	8 potential glycosylation sites			8 potential glycosylation sites						sites	hCG has 8 potential glycosylation sites leading to a high number of isoforms (including glycoforms and other post-translational modifications) that we are interesting to characterize.
30581149	0	47	gly	glycoproteins	12:24	arg1	Recombinant glycoproteins	Recombinant glycoproteins				Fterm		glycoproteins			Recombinant glycoproteins resembling carbohydrate-specific IgE epitopes from plants, venoms and mites.
32699088	11	39	gly	glycosylation	2126:2138	arg2	the N-linked glycosylation sites			the N-linked glycosylation sites						sites	Experimental analyses verified that the N-linked glycosylation sites on the NA head domain contribute to virion incorporation and replication.
29491151	7	46	gly	glycosylation	1448:1460	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	The TF viruses were characterized by shorter V1V2 regions, a reduced number of potential N-linked glycosylation sites, and a higher infectivity titer compared to the virus variants from the adults in the chronic stage of infection.
32258897	0	48	gly	Heterogeneity	14:26	arg1	rhIFN-β	rhIFN-β				Cterm		rhIFN-β	3456		Glycosylation Heterogeneity of Hyperglycosylated Recombinant Human Interferon-β (rhIFN-β).
32258897	0	48	gly	Heterogeneity	14:26	arg1	Hyperglycosylated Recombinant Human Interferon-β	Hyperglycosylated Recombinant Human Interferon-β				PUBTATOR		Human Interferon-β 	3456		Glycosylation Heterogeneity of Hyperglycosylated Recombinant Human Interferon-β (rhIFN-β).
32258897	0	57	gly	Hyperglycosylated	31:47	arg1	rhIFN-β	rhIFN-β				Cterm		rhIFN-β	3456		Glycosylation Heterogeneity of Hyperglycosylated Recombinant Human Interferon-β (rhIFN-β).
32258897	0	57	gly	Hyperglycosylated	31:47	arg1	Hyperglycosylated Recombinant Human Interferon-β	Hyperglycosylated Recombinant Human Interferon-β				PUBTATOR		Human Interferon-β 	3456		Glycosylation Heterogeneity of Hyperglycosylated Recombinant Human Interferon-β (rhIFN-β).
30028870	8	45	gly	glycosylation	1461:1473	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Mutagenesis experiments suggest that the α and β subunits can tolerate large truncations in the non-conserved M3/M4 cytoplasmic loop without compromising oligomeric assembly or GABA-gated channel activity, although removal of N-linked glycosylation sites is negatively correlated with expression level.
33167210	3	16	gly	glycosylation	519:531	arg1	PSA	PSA				PUBTATOR		PSA	354		To understand the involvement of PSA glycosylation in the fertilization process, analytical methods are required to study the glycosylation of PSA from seminal plasma with a high glycoform resolution and in a protein-specific manner.
34341581	0	16	gly	glycoproteins	68:80	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			StrucGP: de novo structural sequencing of site-specific N-glycan on glycoproteins using a modularization strategy.
29530619	0	73	gly	Nav1.5	27:32	arg1	O-GlcNAcylation	Nav1.5			O-GlcNAcylation	PUBTATOR		Nav1.5	25665		O-GlcNAcylation of cardiac Nav1.5 contributes to the development of arrhythmias in diabetic hearts.
33300777	4	26	gly	glycoprotein	779:790	arg1	a native glycoprotein	a native glycoprotein				Fterm		glycoprotein			In this method, using peptide-imprinted magnetic nanoparticles (MNPs) as a versatile platform, a glycopeptide tryptically digested from a native glycoprotein was selectively entrapped for positive selection, while a nonglycosylated analogue with an identical peptide sequence was synthesized for negative selection.
33300777	4	28	gly	glycopeptide	731:742	arg2	a glycopeptide			a glycopeptide						glycopeptide	In this method, using peptide-imprinted magnetic nanoparticles (MNPs) as a versatile platform, a glycopeptide tryptically digested from a native glycoprotein was selectively entrapped for positive selection, while a nonglycosylated analogue with an identical peptide sequence was synthesized for negative selection.
29237830	13	40	gly	LASV	2031:2034	arg1	dystroglycan	LASV			dystroglycan	Cterm		LASV			Although the principal LASV receptor, dystroglycan (DG), is ubiquitously expressed, virus binding critically depends on DG's posttranslational modification, which does not always correlate with tissue tropism.
32220931	4	49	gly	glycopeptide	916:927	arg2	glycopeptide			glycopeptide						glycopeptide	Here we describe several structures of mouse and human FUT8 in the apo state and in complex with GDP, a mimic of the donor substrate, and with a glycopeptide acceptor substrate at 1.80-2.50 Å resolution.
31604106	16	41	gly	O-glycosylation	2799:2813	arg1	recombinant hCG protein	recombinant hCG protein				PUBTATOR		hCG protein	93659		This study revealed the major limiting factors of O-glycosylation of recombinant hCG protein in CHO cells and proposed an effective expression regulation strategy.
26040437	2	91	gly	six-histidine	448:460	arg1	six-histidine (6xHis) tag			histidine	six-histidine (6xHis) tag					histidine	Biosynthesis, glycosylation and secretion of the HA1 proteins, with natural or a melittin signal peptide at the N-terminus and a six-histidine (6xHis) tag at the C-terminus, were examined in insect cells.
26040437	2	96	gly	glycosylation	333:345	arg1	the HA1 proteins	the HA1 proteins				PUBTATOR		HA1 proteins	23526		Biosynthesis, glycosylation and secretion of the HA1 proteins, with natural or a melittin signal peptide at the N-terminus and a six-histidine (6xHis) tag at the C-terminus, were examined in insect cells.
32432921	7	17	gly	N-glycosylation	1029:1043	arg2	the N-glycosylation site	protein		site		Fterm		protein		site	In this work, we have determined the quantitative distribution of AF-hSP-D oligomers, characterized the sugars attached through the N-glycosylation site of the protein, and compared the activity of hSP-D from AF and PAP with respect to their ability to bind and agglutinate bacteria.
33273015	6	76	gly	glycopeptide	850:861	arg2	glycopeptide profiling			glycopeptide profiling						glycopeptide	Native mass spectrometry, mass photometry and glycopeptide profiling revealed significant molecular complexity of diprotomeric nMPO arising from heterogeneous glycosylation, oxidation, chlorination and polypeptide truncation variants and a previously unreported low-abundance monoprotomer.
28939828	3	7	gly	glycoprotein	372:383	arg1	glycoprotein glucosyltransferase				glycoprotein glucosyltransferase						The ER folding-sensor enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) acts as a gatekeeper in the ER quality control system by specifically catalysing monoglucosylation onto incompletely folded glycoproteins, thereby enabling them to interact with lectin-chaperone complexes.
28939828	3	25	gly	enzyme	353:358	arg1	The ER folding-sensor enzyme UDP-glucose	enzyme			The ER folding-sensor enzyme UDP-glucose	Fterm		enzyme			The ER folding-sensor enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) acts as a gatekeeper in the ER quality control system by specifically catalysing monoglucosylation onto incompletely folded glycoproteins, thereby enabling them to interact with lectin-chaperone complexes.
28939828	3	67	gly	glycoproteins	536:548	arg1	incompletely folded glycoproteins	incompletely folded glycoproteins				Fterm		glycoproteins			The ER folding-sensor enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) acts as a gatekeeper in the ER quality control system by specifically catalysing monoglucosylation onto incompletely folded glycoproteins, thereby enabling them to interact with lectin-chaperone complexes.
34495528	1	30	gly	N-glycosylated	108:121	arg1	more than 7000 proteins	more than 7000 proteins				Fterm		proteins			N-glycosylation is a highly conserved glycan modification, and more than 7000 proteins are N-glycosylated in humans.
31831633	2	37	gly	glycosylated	400:411	arg1	this abnormally glycosylated MUC1	this abnormally glycosylated MUC1				OGER		MUC1	P15941		Changes in glycosylation of the oncoprotein MUC1 commonly occur in chronic inflammation, including ulcerative colitis, and this abnormally glycosylated MUC1 promotes cancer development and progression.
31831633	2	62	gly	glycosylation	272:284	arg1	the oncoprotein MUC1	the oncoprotein MUC1				OGER		MUC1	P15941		Changes in glycosylation of the oncoprotein MUC1 commonly occur in chronic inflammation, including ulcerative colitis, and this abnormally glycosylated MUC1 promotes cancer development and progression.
31972267	1	4	gly	attached	318:325	arg1	the protein AND the glycan-moiety	the protein			the glycan-moiety	Fterm		protein			Cell surface glycoproteins play critical roles in maintaining cardiac structure and function in health and disease and the glycan-moiety attached to the protein is critical for proper protein folding, stability and signaling [1].
31972267	1	16	gly	glycoproteins	194:206	arg1	Cell surface glycoproteins	Cell surface glycoproteins				Fterm		glycoproteins			Cell surface glycoproteins play critical roles in maintaining cardiac structure and function in health and disease and the glycan-moiety attached to the protein is critical for proper protein folding, stability and signaling [1].
31604106	11	12	gly	polypeptide	1857:1867	arg1	Galnt1	polypeptide N-acetylgalactosaminyltransferase1			Galnt1	PUBTATOR		polypeptide N-acetylgalactosaminyltransferase1	2589		Furthermore, the effects and mechanisms of the key genes of O-glycan sugar chain synthesis and hydrolases such as polypeptide N-acetylgalactosaminyltransferase1 (Galnt1), Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase (C1galt1), O-linked N-acetylglucosamine transferase (Ogt) and Hexosaminidase (Hex), were evaluated.
30852271	6	39	gly	glycosylation	1019:1031	arg1	the IBV M protein ectodomain			ectodomain						ectodomain	Taken together, this study demonstrates that although not essential for replication, glycosylation in the IBV M protein ectodomain plays important roles in activating ER stress, apoptosis and proinflammatory response, and may contribute to the pathogenesis of IBV.
33030205	5	1	gly	O-glycopeptide	912:925	arg2	an O-glycopeptide			an O-glycopeptide						O-glycopeptide	Here, through the determination of a crystal structure of IMPa in complex with an O-glycopeptide, we reveal that the N-terminal domain of IMPa, which is classified in Pfam as IMPa_N_2, is a proline recognition domain that also shows the properties of recognizing an O-linked glycan on the serine/threonine residue following the proline.
33030205	5	47	gly	glycan	1105:1110	arg1	the serine/threonine residue			the serine/threonine residue	the serine/threonine residue		Site			residue	Here, through the determination of a crystal structure of IMPa in complex with an O-glycopeptide, we reveal that the N-terminal domain of IMPa, which is classified in Pfam as IMPa_N_2, is a proline recognition domain that also shows the properties of recognizing an O-linked glycan on the serine/threonine residue following the proline.
31029427	9	11	gly	site	1502:1505	arg1	FOXA1	FOXA1			site	PUBTATOR		FOXA1	3169		Additionally, we performed ESI-ETD-MS/MS analysis of the full-length O-GalNAcylated FOXA1 protein and identified S355 as the O-GalNAc modification site on FOXA1, consistent with the peptide reaction.
32143591	5	14	gly	-glycoprotein	837:849	arg1	mannosyl(α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase	mannosyl(α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase				Fterm		-glycoprotein			Moreover, we performed immunohistochemical staining of mannosyl(α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase (MGAT1), which is an essential glycosyltransferase that converts high-mannose glycans to complex- or hybrid-type N-glycans.
30044221	5	15	gly	glycosylation	929:941	arg1	the α1 subunit	the α1 subunit				PUBTATOR		1 subunit	146		Remarkable N-linked glycosylation on the α1 subunit occludes the extracellular vestibule of the ion channel and is poised to modulate receptor assembly and perhaps ion channel gating.
33998969	3	52	gly	hyper-glycosylated	597:614	arg1	hyper-glycosylated variants	hyper-glycosylated variants				Fterm		variants			Glycoengineering is a favorably used strategy to design and generate hyper-glycosylated variants.
33300777	7	76	gly	glycoprotein	1268:1279	arg1	the glycoprotein transferrin	the glycoprotein transferrin				Fterm		glycoprotein			With the glycoprotein transferrin as a source of target glycan, two satisfied anti-A2G2S2 aptamers were selected within seven rounds.
32627435	2	25	gly	afucosylated	402:413	arg1	afucosylated N-linked glycan structures				afucosylated N-linked glycan structures						It has been well established that higher levels of afucosylated N-linked glycan structures on the Fc region enhance the IgG binding affinity to the FcγIIIa receptor and lead to increased ADCC activity.
32627435	2	38	gly	structures	431:440	arg1	the Fc region			the Fc region	the Fc region		Site			region	It has been well established that higher levels of afucosylated N-linked glycan structures on the Fc region enhance the IgG binding affinity to the FcγIIIa receptor and lead to increased ADCC activity.
32326134	5	29	gly	O-glycosylation	1153:1167	arg2	O-glycosylation sites			O-glycosylation sites						sites	O-glycosylation sites were identified using O-glycopeptides (bold underlined; 56SGETRTSVI, 259SHSSSGRSRTI, 272GSPSSVSSAEQI, 307RPSYGAL, 625QTLGPL, 728TMTTRTSVVV, and 1080RPEDNTAVA) obtained from proteolytic BSM; these sites are in the four domains of BSM.
32326134	5	11	gly	O-glycopeptides	1197:1211	arg1	O-glycopeptides			O-glycopeptides						O-glycopeptides	O-glycosylation sites were identified using O-glycopeptides (bold underlined; 56SGETRTSVI, 259SHSSSGRSRTI, 272GSPSSVSSAEQI, 307RPSYGAL, 625QTLGPL, 728TMTTRTSVVV, and 1080RPEDNTAVA) obtained from proteolytic BSM; these sites are in the four domains of BSM.
33116178	7	48	gly	fucosylated	1146:1156	arg1	specific fucosylated glycan motifs			specific fucosylated glycan motifs							This knowledge provides a starting point for investigations into the role of specific fucosylated glycan motifs of schistosomes in parasite-host interactions.
31094416	4	14	gly	glycosylation	949:961	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Even though the canonical sugar-binding β-sheet S-face (β-strands 1, 10, 3, 4, 5, 6) of the Gal-3 β-sandwich is involved in interactions with CD146 (e.g. N-linked glycosylation sites), equivalent HSQC spectral perturbations at residues on the opposing Gal-3 F-face β-sheet (β-strands 11, 2, 7, 8, 9) indicate involvement of the Gal-3 F-face in binding CD146.
30514763	0	39	gly	O-glycosylation	40:54	arg1	the adhesin MIC2	the adhesin MIC2				OGER		MIC2	P14209		Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection.
28348171	7	46	gly	glycopeptides	1216:1228	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	To identify enriched surface glycoproteins, we then performed solid-phase extraction of N-linked glycopeptides followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on our homogeneous macrophage populations.
28348171	7	63	gly	glycoproteins	1148:1160	arg1	enriched surface glycoproteins	enriched surface glycoproteins				Fterm		glycoproteins			To identify enriched surface glycoproteins, we then performed solid-phase extraction of N-linked glycopeptides followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on our homogeneous macrophage populations.
30392906	8	9	gly	glycosylation	960:972	arg1	Asn50			Asn50				Myelin protein zero-like protein 1	9019	Asn50	Our results demonstrate that glycosylation of Asn50 is essential for its function as a receptor of ConA.
33103998	1	6	gly	glycosylated	194:205	arg1	its receptor-binding domain	ACE2		domain		OGER		ACE2	Q9BYF1	domain	The Spike protein of SARS-CoV-2, its receptor-binding domain (RBD), and its primary receptor ACE2 are extensively glycosylated.
33103998	1	6	gly	glycosylated	194:205	arg1	its receptor-binding domain	SARS		domain		OGER		SARS	P49591	domain	The Spike protein of SARS-CoV-2, its receptor-binding domain (RBD), and its primary receptor ACE2 are extensively glycosylated.
33103998	1	6	gly	glycosylated	194:205	arg1	its receptor-binding domain	protein		domain		Fterm		protein		domain	The Spike protein of SARS-CoV-2, its receptor-binding domain (RBD), and its primary receptor ACE2 are extensively glycosylated.
33340519	5	9	gly	glycoforms	730:739	arg1	engineered ACE2 glycoforms	engineered ACE2 glycoforms				OGER		ACE2	Q9BYF1		We generated a panel of engineered ACE2 glycoforms which were analyzed by mass spectrometry to reveal the site-specific glycan modifications.
30305605	2	55	gly	glycosylation	303:315	arg1	proteins	proteins				Fterm		proteins	59048		Thus, it plays a crucial role in the biosynthesis of O-linked glycosylation of proteins.
30633504	4	40	gly	monoglycosylated	779:794	arg1	RNase 1	RNase 1				PUBTATOR		RNase 1	6035		As a glutamine residue is not a substrate for cellular oligosaccharyltransferase, we used strategic asparagine-to-glutamine substitutions to produce the three diglycosylated and three monoglycosylated forms of RNase 1.
30633504	4	55	gly	diglycosylated	754:767	arg1	RNase 1	RNase 1				PUBTATOR		RNase 1	6035		As a glutamine residue is not a substrate for cellular oligosaccharyltransferase, we used strategic asparagine-to-glutamine substitutions to produce the three diglycosylated and three monoglycosylated forms of RNase 1.
30250045	1	60	gly	sialylated	189:198	arg1	the sialylated protein HEG homolog 1	the sialylated protein HEG homolog 1				PUBTATOR		HEG homolog 1	57493		The anti-mesothelioma mAb SKM9-2 recognizes the sialylated protein HEG homolog 1 (HEG1).
30250045	1	60	gly	sialylated	189:198	arg1	HEG1	HEG1				PUBTATOR		HEG1	Q9ULI3		The anti-mesothelioma mAb SKM9-2 recognizes the sialylated protein HEG homolog 1 (HEG1).
29258330	2	25	gly	glycosylation	494:506	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites, sequence	From inspection of aligned HIV-2 sequences, we verified that V1/V2 region showed the highest degree of amino acid sequence heterogeneity, including polymorphisms in N-linked glycosylation sites, sequence, and length.
27666939	9	36	gly	afucosylated	1731:1742	arg1	primarily fucosylated to afucosylated glycans				primarily fucosylated to afucosylated glycans						Finally, FXKO clones can express antibodies with the desired ratio of primarily fucosylated to afucosylated glycans when fucose is titrated into the production media, to allow achieving intended levels of FcγRIII-binding and ADCC for an antibody.
29569918	7	77	gly	unsialylated	1251:1262	arg1	unsialylated O-glycans				unsialylated O-glycans						Our double labeling technique identified significant differences in unsialylated O-glycans between B-cell and T-cell lymphomas and between B-cell lymphomas and normal adjacent lymph nodes.
34343291	0	67	gly	glycoprotein	44:55	arg1	glycoprotein dynamics	glycoprotein dynamics				Fterm		glycoprotein			Effects of changes in glycan composition on glycoprotein dynamics: example of N-glycans on insulin receptor.
34662441	8	77	gly	glycoforms	1647:1656	arg1	PSMA	PSMA				OGER		PSMA	Q04609		CONCLUSIONS Our study presents initial descriptive analysis of the glycoforms of PSMA observed in cell lines and in prostate tissue.
30479582	2	4	gly	glycosylation	433:445	arg1	recombinant IgG	recombinant IgG				Cterm		IgG			This task is essential in the biotherapeutics industry, where the type and amount of glycosylation on recombinant IgG alter the efficacy, function, and immunogenicity.
30889014	6	24	gly	N-glycosite	949:959	arg2	the online N-glycosite software			the online N-glycosite software						N-glycosite	Potential N-linked glycosylation site (PNGS) number was calculated using the online N-glycosite software.
30889014	6	77	gly	glycosylation	884:896	arg2	Potential N-linked glycosylation site			Potential N-linked glycosylation site						site	Potential N-linked glycosylation site (PNGS) number was calculated using the online N-glycosite software.
31308178	3	61	gly	N-glycosylated	542:555	arg1	VEGFR2	VEGFR2				PUBTATOR		VEGFR2	3791		VEGFR2 is a highly N-glycosylated receptor tyrosine kinase involved in pro-angiogenic signaling in physiological and pathological contexts, including cancer.
31308178	3	61	gly	N-glycosylated	542:555	arg1	a highly N-glycosylated receptor tyrosine kinase	a highly N-glycosylated receptor tyrosine kinase				Fterm		kinase			VEGFR2 is a highly N-glycosylated receptor tyrosine kinase involved in pro-angiogenic signaling in physiological and pathological contexts, including cancer.
31722217	2	61	gly	has	336:338	arg1	Notch AND 22 O-fucosylation sites	Notch			22 O-fucosylation sites	PUBTATOR		Notch	31293		Although Notch has 22 O-fucosylation sites, the biologically relevant sites affecting Notch activity during animal development in vivo in the presence or absence of Fringe are not known.
30032777	5	7	gly	glycopeptides	789:801	arg2	glycopeptides			glycopeptides						glycopeptides	The enrichment efficiency of MAR@MOP to glycopeptides was demonstrated by trapping N-linked glycopeptides from tryptic digests of human immunoglobulin G (IgG), horseradish peroxidase (HRP) and bovine fetuin.
30032777	5	34	gly	glycopeptides	841:853	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	The enrichment efficiency of MAR@MOP to glycopeptides was demonstrated by trapping N-linked glycopeptides from tryptic digests of human immunoglobulin G (IgG), horseradish peroxidase (HRP) and bovine fetuin.
30032777	5	38	gly	trapping	823:830	arg1	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	The enrichment efficiency of MAR@MOP to glycopeptides was demonstrated by trapping N-linked glycopeptides from tryptic digests of human immunoglobulin G (IgG), horseradish peroxidase (HRP) and bovine fetuin.
34379416	11	80	gly	glycoproteins	1808:1820	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We conclude that glycosylation is fundamental for protein dynamics, making it a necessity for a truthful representation of the flexibility and function in in silico studies of glycoproteins.
34532545	2	56	gly	protein	376:382	arg1	the O-GlcNAcylation level	protein			the O-GlcNAcylation level	Fterm		protein			Evaluation of the O-GlcNAcylation level of a specific protein, however, needs multiple and time-consuming steps if using conventional methods (e.g., immune-purification, mass spectrometric analysis).
32039451	0	34	gly	strain	53:58	arg1	The N-glycans	strain			The N-glycans	Fterm		strain			The N-glycans of Chlorella sorokiniana and a related strain contain arabinose but have strikingly different structures.
28366604	4	55	gly	glycosylation	690:702	arg2	N277			site N277						site N277	We identified a highly conserved N-linked glycosylation site N277 in the C2 region which strongly affected the immunogenicity of the CD4i Ab domain.
33577335	3	1	gly	glycosylated	470:481	arg1	IL-17A	IL-17A				PUBTATOR		IL-17A	3605		Here we report our synthesis and evaluation of homogeneously glycosylated interleukin-17A (IL-17A), based on a synthetic approach combining solid-phase synthesis of (glyco)peptides, chemoenzymatic glycan modification on segments, and chemical ligations.
33577335	3	1	gly	glycosylated	470:481	arg1	homogeneously glycosylated interleukin-17A	homogeneously glycosylated interleukin-17A				PUBTATOR		interleukin-17A	3605		Here we report our synthesis and evaluation of homogeneously glycosylated interleukin-17A (IL-17A), based on a synthetic approach combining solid-phase synthesis of (glyco)peptides, chemoenzymatic glycan modification on segments, and chemical ligations.
33577335	3	21	gly	peptides	581:588	arg1	chemoenzymatic glycan modification			peptides	chemoenzymatic glycan modification					peptides	Here we report our synthesis and evaluation of homogeneously glycosylated interleukin-17A (IL-17A), based on a synthetic approach combining solid-phase synthesis of (glyco)peptides, chemoenzymatic glycan modification on segments, and chemical ligations.
33577335	3	43	gly	modification	613:624	arg1	(glyco)peptides AND chemoenzymatic glycan modification			(glyco)peptides	chemoenzymatic glycan modification					peptides	Here we report our synthesis and evaluation of homogeneously glycosylated interleukin-17A (IL-17A), based on a synthetic approach combining solid-phase synthesis of (glyco)peptides, chemoenzymatic glycan modification on segments, and chemical ligations.
33947960	3	59	gly	ErbB2	585:589	arg1	the site-specific glycan repertoire	ErbB2			the site-specific glycan repertoire	PUBTATOR		ErbB2	2064		However, the site-specific glycan repertoire of ErbB2, as well as the detailed molecular mechanisms through which specific aberrant glycan signatures functionally impact the malignant features of ErbB2-addicted GC cells, including the acquisition of trastuzumab resistance, remain elusive.
34278967	2	41	gly	glycosylated	329:340	arg1	S protein	S protein				PUBTATOR		S protein	Q15517		S protein is heavily glycosylated and the glycosylation sites are relatively conserved, thus glycans on S protein surface could be a target for the development of anti-SARS-CoV-2 strategies against variants.
34278967	2	88	gly	glycosylation	350:362	arg2	the glycosylation sites			the glycosylation sites						sites	S protein is heavily glycosylated and the glycosylation sites are relatively conserved, thus glycans on S protein surface could be a target for the development of anti-SARS-CoV-2 strategies against variants.
29526322	7	14	gly	Glycosylation	858:870	arg1	NCX3	NCX3		N45 site		PUBTATOR		NCX3	6547	N45 site	Glycosylation of NCX3 at the N45 site was required for targeting the protein to the plasma membrane, and the N45 site functioned as an on-off switch for the translocation of NCX3 to either the plasma membrane or the membrane of the ER.
29246839	5	56	gly	glycoprotein	1002:1013	arg1	a monomeric glycoprotein	a monomeric glycoprotein				Fterm		glycoprotein			Sa1-SSTrec purified from the culture supernatant was a monomeric glycoprotein optimally active at pH 5.0-6.0 and 45-50 °C.
29246839	5	56	gly	glycoprotein	1002:1013	arg1	Sa1-SSTrec	Sa1-SSTrec				OGER		Sa1	Q8WVM7		Sa1-SSTrec purified from the culture supernatant was a monomeric glycoprotein optimally active at pH 5.0-6.0 and 45-50 °C.
34341581	6	44	gly	glycoproteins	1088:1100	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The approach will be greatly beneficial for in-depth structural and functional study of glycoproteins in the biomedical research.
32782472	3	50	gly	N-glycosylated	502:515	arg1	an N-glycosylated catalytic domain			an N-glycosylated catalytic domain						domain	The architecture of TrCel7A includes an N-glycosylated catalytic domain, which is connected to a carbohydrate-binding module through a flexible, O-glycosylated linker.
30021839	1	18	gly	glycans	293:299	arg1	misfolded proteins	proteins			glycans	Fterm		proteins			Endoplasmic reticulum (ER) degradation-enhancing α-mannosidase-like 1 protein (EDEM1) is a protein quality control factor that was initially proposed to recognize N-linked glycans on misfolded proteins through its mannosidase-like domain (MLD).
30054538	11	31	gly	desialylated	1950:1961	arg1	partially desialylated PrPSc	partially desialylated PrPSc				PUBTATOR		PrPSc	19122		Moreover, transient degradation of Iκβα observed upon treatment with partially desialylated PrPSc suggests that canonical NFκB activation pathway is involved in inflammatory response.
31270739	6	57	gly	glycopeptide	946:957	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	By developing workflows for glycopeptide enrichment and identification we have demostrated that it is now possible to characterise the glycoproteomes of microbial species in a truely high-throughput manner.
31837192	1	82	gly	glycoproteins	259:271	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			Glyco-design of proteins is a powerful tool in fundamental studies of structure-function relationship and in obtaining profiles optimized for efficacy of therapeutic glycoproteins.
36303733	9	69	gly	protein	1598:1604	arg1	the N-glycan profile	S) protein			the N-glycan profile	OGER		S) protein	P04004		Heterogeneity in the N-glycan profile of the spike (S) protein and its potential effect on vaccine efficacy or adverse reactions to the vaccines remain unexplored.
28657654	7	12	gly	proteins	1446:1453	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins			These findings suggest that dysregulation of O-GlcNAcylation of multiple brain proteins may be involved in the development of sporadic AD.
29779369	6	39	gly	profiles	1204:1211	arg1	human serum proteins	proteins			profiles	Fterm		proteins			With optimized N-glycan release conditions, glycosylamine labeling conditions, and MS acquisition parameters, the N-glycan profiles and abundances in human serum proteins of cancer patients before and after chemotherapy were compared.
30205382	7	27	gly	p727STAT3	1251:1259	arg1	p727STAT3 expression	STAT3			p727STAT3 expression	PUBTATOR		STAT3	6774		While O-GlcNAcylation inhibited p727STAT3 expression, augmented O-GlcNAcylation could balance p705STAT3 expression within relatively high levels corresponding to vascular endothelial growth factor (VEGF) changes.
34888356	7	40	gly	compositions	1548:1559	arg1	sialic acid epitopes			sialic acid epitopes	sialic acid epitopes						Then, three sialylated biantennary N-glycans were further subjected to the separation of linkage isomers and quantification using porous graphitized carbon-liquid chromatography (PGC-LC)/multiple reaction monitoring (MRM)-MS. We were able to successfully identify 11 isomers with sialic acid epitopes from the three glycan compositions consisting of Hex5HexNAc4NeuAc1, Hex5HexNAc4Fuc1NeuAc1, and Hex5HexNAc4NeuAc2.
34888356	7	49	gly	sialylated	1237:1246	arg1	three sialylated biantennary N-glycans				three sialylated biantennary N-glycans						Then, three sialylated biantennary N-glycans were further subjected to the separation of linkage isomers and quantification using porous graphitized carbon-liquid chromatography (PGC-LC)/multiple reaction monitoring (MRM)-MS. We were able to successfully identify 11 isomers with sialic acid epitopes from the three glycan compositions consisting of Hex5HexNAc4NeuAc1, Hex5HexNAc4Fuc1NeuAc1, and Hex5HexNAc4NeuAc2.
30535277	5	23	gly	glycoproteins	894:906	arg1	the fucosylated glycoproteins	the fucosylated glycoproteins				Fterm		glycoproteins			Besides, the fucosylated glycoproteins were isolated from pooled saliva of HV, HB, HC, and HCC by LTL-magnetic particle conjugates.
30535277	5	66	gly	fucosylated	882:892	arg1	the fucosylated glycoproteins	the fucosylated glycoproteins				Fterm		glycoproteins			Besides, the fucosylated glycoproteins were isolated from pooled saliva of HV, HB, HC, and HCC by LTL-magnetic particle conjugates.
31101650	3	13	gly	glycosylation	548:560	arg1	cell surface glycoproteins	cell surface glycoproteins				Fterm		glycoproteins			We used selective exo-enzymatic labeling of cells deficient in the two catalytic subunits of oligosaccharyltransferase - STT3A and STT3B - to monitor the presence and glycosylation status of cell surface glycoproteins.
31101650	3	21	gly	glycoproteins	585:597	arg1	cell surface glycoproteins	cell surface glycoproteins				Fterm		glycoproteins			We used selective exo-enzymatic labeling of cells deficient in the two catalytic subunits of oligosaccharyltransferase - STT3A and STT3B - to monitor the presence and glycosylation status of cell surface glycoproteins.
34200965	7	60	gly	glycopeptide	976:987	arg2	a glycopeptide			glycopeptide	the glycan part					glycopeptide	Since alterations in the glycan part of a glycopeptide cause mass alterations, analytical size exclusion chromatography was applied to detect these mass shifts.
31852790	0	100	gly	Glycans	9:15	arg1	Hemagglutinin	Hemagglutinin			Glycans	Fterm		Hemagglutinin			N-Linked Glycans and K147 Residue on Hemagglutinin Synergize To Elicit Broadly Reactive H1N1 Influenza Virus Antibodies.
29339411	10	38	gly	glycopeptides	1949:1961	arg2	glycopeptides			glycopeptides						glycopeptides	CID oxonium ions and electron transfer dissociation, however, confirmed that just a single site was glycosylated, showing that glycan-to-peptide rearrangement can occur on glycopeptides and that this effect is influenced by the molecular nature of the glycan moiety.
29339411	10	85	gly	glycosylated	1877:1888	arg1	just a single site			just a single site						site	CID oxonium ions and electron transfer dissociation, however, confirmed that just a single site was glycosylated, showing that glycan-to-peptide rearrangement can occur on glycopeptides and that this effect is influenced by the molecular nature of the glycan moiety.
28534487	6	1	gly	integrin	1004:1011	arg1	an N-linked sugar	integrin			an N-linked sugar	Fterm		integrin			In addition, an N-linked sugar of the integrin attaches to the previously identified HS binding site, suggesting a functional role.
28534487	6	28	gly	attaches	1013:1020	arg1	the previously identified HS binding site AND an N-linked sugar			the previously identified HS binding site	an N-linked sugar					site	In addition, an N-linked sugar of the integrin attaches to the previously identified HS binding site, suggesting a functional role.
32220931	1	0	gly	Fucosylation	81:92	arg1	the innermost GlcNAc				the innermost GlcNAc						Fucosylation of the innermost GlcNAc of N-glycans by fucosyltransferase 8 (FUT8) is an important step in the maturation of complex and hybrid N-glycans.
34885895	5	16	gly	glycosylation	732:744	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem.
32938717	3	61	gly	epitope	588:594	arg1	a high-affinity epitope tag				a high-affinity epitope tag						Here, we established a purification method for mutated OSTs using a high-affinity epitope tag attached to the catalytic subunit Stt3, from yeast cells co-expressing the WT OST to support growth.
32938717	3	73	gly	attached	600:607	arg1	the catalytic subunit Stt3 AND a high-affinity epitope tag	the catalytic subunit Stt3			a high-affinity epitope tag	PUBTATOR		Stt3	852862		Here, we established a purification method for mutated OSTs using a high-affinity epitope tag attached to the catalytic subunit Stt3, from yeast cells co-expressing the WT OST to support growth.
29886537	7	17	gly	status	1122:1127	arg1	PrPSc	PrPSc			status	PUBTATOR		PrPSc	19122		The current chapter describes the procedure for the analysis of sialylation status of PrPSc from various sources including central nervous system, secondary lymphoid organs, cultured cells, or PrPSc produced in Protein Misfolding Cyclic Amplification.
29886537	7	45	gly	PrPSc	1132:1136	arg1	sialylation status	PrPSc			sialylation status	PUBTATOR		PrPSc	19122		The current chapter describes the procedure for the analysis of sialylation status of PrPSc from various sources including central nervous system, secondary lymphoid organs, cultured cells, or PrPSc produced in Protein Misfolding Cyclic Amplification.
29886537	7	55	gly	sialylation	1110:1120	arg1	PrPSc	PrPSc				PUBTATOR		PrPSc	19122		The current chapter describes the procedure for the analysis of sialylation status of PrPSc from various sources including central nervous system, secondary lymphoid organs, cultured cells, or PrPSc produced in Protein Misfolding Cyclic Amplification.
30158294	6	45	gly	N-glycosylation	861:875	arg2	a conserved N-glycosylation site	SERINC5		site, N294				SERINC5	256987	site, N294	Sequence alignment of SERINC family proteins led us to identify a conserved N-glycosylation site, N294, in SERINC5.
31094416	1	80	gly	glycoprotein	209:220	arg1	cell adhesion glycoprotein CD146	cell adhesion glycoprotein CD146				Fterm		glycoprotein			Galectin-3 (Gal-3) binds to cell adhesion glycoprotein CD146 to promote cytokine secretion and mediate endothelial cell migration.
34232571	4	54	gly	glycosylation	586:598	arg2	glycosylation sites			glycosylation sites						sites	In this protocol, we detail a workflow for comprehensive analyses of intact glycopeptides (IGPs), glycosylation sites, and glycans from N-linked glycoproteins.
34232571	4	19	gly	glycopeptides	564:576	arg1	N-linked glycoproteins	glycoproteins		glycopeptides		Fterm		glycoproteins		glycopeptides	In this protocol, we detail a workflow for comprehensive analyses of intact glycopeptides (IGPs), glycosylation sites, and glycans from N-linked glycoproteins.
34232571	4	25	gly	glycoproteins	633:645	arg1	N-linked glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			In this protocol, we detail a workflow for comprehensive analyses of intact glycopeptides (IGPs), glycosylation sites, and glycans from N-linked glycoproteins.
29531238	4	45	gly	glycoproteins	820:832	arg1	4,412 glycoproteins	4,412 glycoproteins				Fterm		glycoproteins			Only 27.4% of all NLGs are experimentally validated sites on 4,412 glycoproteins.
33263330	0	33	gly	N-glycosylated	14:27	arg1	Homogeneously N-glycosylated proteins	Homogeneously N-glycosylated proteins				Fterm		proteins			Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis.
33560857	6	26	gly	N-glycopeptides	1078:1092	arg2	893 different N-glycopeptides			893 different N-glycopeptides						N-glycopeptides	In total, 893 different N-glycopeptides and 128 unique N-glycan compositions were identified.
33404348	0	48	gly	Glycoproteins	41:53	arg1	Glycan Alterations	Glycoproteins			Glycan Alterations	Fterm		Glycoproteins			The Repertoire of Glycan Alterations and Glycoproteins in Human Cancers.
30054538	0	26	gly	sialylation	62:72	arg1	PrPSc	PrPSc				PUBTATOR		PrPSc	19122		Inflammatory response of microglia to prions is controlled by sialylation of PrPSc.
30054538	0	86	gly	PrPSc	77:81	arg1	sialylation	PrPSc			sialylation	PUBTATOR		PrPSc	19122		Inflammatory response of microglia to prions is controlled by sialylation of PrPSc.
30619255	2	107	gly	asialylated	461:471	arg1	the asialylated disaccharide Gal-β1,3-GalNAc-Ser/Thr			the asialylated disaccharide Gal-β1,3-GalNAc-Ser/Thr						Gal-β1,3-GalNAc-Ser/Thr	For decades, GC B cells have been defined by their reactivity to the plant lectin peanut agglutinin (PNA), which binds serine/threonine (O-linked) glycans containing the asialylated disaccharide Gal-β1,3-GalNAc-Ser/Thr (also called T-antigen).
29315243	7	51	gly	Runx2	1315:1319	arg1	O-GlcNAcylation	Runx2			O-GlcNAcylation	PUBTATOR		Runx2	12393		Cell treatment with high glucose, glucosamine or N-acetylglucosamine increased O-GlcNAcylation of Runx2 and the total levels of O-GlcNAcylated proteins, which led to a decrease in the transcriptional activity of Runx2, expression levels of osteogenic marker genes (Runx2, osterix, alkaline phosphatase, and type I collagen), and activity of alkaline phosphatase.
34110173	0	72	gly	Glycosylation	39:51	arg1	Erythropoietin	Erythropoietin				PUBTATOR		Erythropoietin	2056		An Integrated Strategy Reveals Complex Glycosylation of Erythropoietin Using Mass Spectrometry.
32441515	9	11	gly	glycopeptide	1553:1564	arg2	glycopeptide			glycopeptide						glycopeptide	This methodology of multiple CE fragmentation without merging spectral information can significantly improve confidence of glycopeptide identification and structural resolution by providing additional information to the established glycopeptide-search algorithms and tools.
30029404	4	70	gly	glycopeptides	894:906	arg2	glycopeptides			glycopeptides						glycopeptides	Thanks to outstanding hydrophilicity brought by the grafted zwitterionic IDA groups, the trap columns showed excellent on-column enrichment performance toward glycopeptides by hydrophilic interaction liquid chromatography (HILIC).
30016717	6	91	gly	containing	1058:1067	arg1	620 peptides AND one or more O-GlcNAc sites			620 peptides	one or more O-GlcNAc sites					peptides	This combination of fractionation and click chemistry is a powerful methodology to map O-GlcNAc sites; indeed, 342 proteins were identified through the identification of 620 peptides containing one or more O-GlcNAc sites.
34229070	2	30	gly	O-glycosylated	322:335	arg1	an O-glycosylated protein	an O-glycosylated protein				Fterm		protein			As an O-glycosylated protein, O-glycosylation of APP is considered to be related to Aβ generation.
34229070	2	83	gly	O-glycosylation	346:360	arg1	APP	APP				OGER		APP	P05067		As an O-glycosylated protein, O-glycosylation of APP is considered to be related to Aβ generation.
28187132	1	20	gly	O-glycosylation	107:121	arg1	serum immunoglobulin A1 (IgA1)	serum immunoglobulin A1 (IgA1)				PUBTATOR		IgA1	P01876		Aberrant O-glycosylation of serum immunoglobulin A1 (IgA1) represents a heritable pathogenic defect in IgA nephropathy, the most common form of glomerulonephritis worldwide, but specific genetic factors involved in its determination are not known.
34341581	2	28	gly	glycopeptides	356:368	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Here, we describe a modularization strategy for de novo interpretation of N-glycan structures on intact glycopeptides using tandem mass spectrometry.
33664361	8	51	gly	N-glycosylation	1238:1252	arg1	viral proteins	viral proteins				Fterm		proteins			Our study clearly demonstrates the progress made towards site-specific N-glycosylation analysis of viral proteins, i.e. for Brazilian ZIKV.
31561469	12	54	gly	glycoprotein	1304:1315	arg1	glycoprotein VI (GPVI)	glycoprotein VI (GPVI)				Fterm		glycoprotein			In contrast, Atr-III cleaves glycoprotein VI (GPVI) into a soluble ~55-kDa fragment (sGPVI).
34857845	6	23	gly	N-glycoforms	1234:1245	arg1	the core fucosylated N-glycoforms				the core fucosylated N-glycoforms						Our results show that a combination of soft fragmentation with exoglycosidases is efficient at the assignment and quantification of the core fucosylated N-glycoforms at specific sites of protein attachment.
34857845	6	87	gly	fucosylated	1222:1232	arg1	the core fucosylated N-glycoforms				the core fucosylated N-glycoforms						Our results show that a combination of soft fragmentation with exoglycosidases is efficient at the assignment and quantification of the core fucosylated N-glycoforms at specific sites of protein attachment.
28487326	3	98	gly	proteins	692:699	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins			A key unfolded protein response prosurvival pathway is controlled by the endoplasmic reticulum stress sensor (inositol-requiring enzyme-1), XBP1 (downstream X-box-binding protein-1), and O-GlcNAc (O-linked β-N-acetylglucosamine) modification of proteins (O-GlcNAcylation).
29304374	1	25	gly	glycoproteins	255:267	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Fucosyltransferase 8 (FUT8) encodes a Golgi-localized α1,6 fucosyltransferase that is essential for transferring the monosaccharide fucose into N-linked glycoproteins, a process known as "core fucosylation."
30420690	3	12	gly	modified	598:605	arg3	the Hedgehog pathway transcription factors AND O-GlcNAcylation	the Hedgehog pathway transcription factors			O-GlcNAcylation	Fterm		factors			In elevated glucose conditions, we determined that the Hedgehog pathway transcription factors, GLI1 and GLI2, are modified by O-GlcNAcylation.
30420690	3	12	gly	modified	598:605	arg3	GLI2 AND O-GlcNAcylation	GLI2			O-GlcNAcylation	PUBTATOR		GLI2	2736		In elevated glucose conditions, we determined that the Hedgehog pathway transcription factors, GLI1 and GLI2, are modified by O-GlcNAcylation.
30420690	3	12	gly	modified	598:605	arg1	GLI1 AND O-GlcNAcylation	GLI1			O-GlcNAcylation	PUBTATOR		GLI1	2735		In elevated glucose conditions, we determined that the Hedgehog pathway transcription factors, GLI1 and GLI2, are modified by O-GlcNAcylation.
31511323	5	82	gly	sialylated	917:926	arg2	Asn-42	β2		Asn-42				2	170589	Asn-42	Using heterologous expression in polarized Madin-Darby canine kidney cells, we show that β2 is N-glycosylated in vivo and in vitro at residues 42, 66, and 74, becoming sialylated only at Asn-42.
31511323	5	58	gly	N-glycosylated	844:857	arg1	β2	2		residues 42, 66, and 74		PUBTATOR		2	170589	residues 42, 66, and 74	Using heterologous expression in polarized Madin-Darby canine kidney cells, we show that β2 is N-glycosylated in vivo and in vitro at residues 42, 66, and 74, becoming sialylated only at Asn-42.
28348171	6	72	gly	glycoproteins	773:785	arg1	novel surface glycoproteins	novel surface glycoproteins				Fterm		glycoproteins			To identify novel surface glycoproteins expressed on M2 macrophages, we developed a novel method of creating homogeneous populations of human macrophages from human CD14+ monocytes in vitro These homogeneous M1 macrophages secrete pro-inflammatory cytokines, and our M2 macrophages secrete anti-inflammatory cytokines as well as vascular endothelial growth factor (VEGF).
33581334	3	11	gly	O-glycoprotein	407:420	arg1	an O-glycoprotein repository	an O-glycoprotein repository				Fterm		O-glycoprotein			To resolve this issue, an O-glycoprotein repository named OGP was established in this work.
29746846	0	25	gly	deposition	30:39	arg1	placental proteins	proteins			deposition	Fterm		proteins			O-linked N-acetyl-glucosamine deposition in placental proteins varies according to maternal glycemic levels.
32887075	5	20	gly	N-glycosylation	786:800	arg2	four N-glycosylation sites			four N-glycosylation sites						sites	The results revealed that there are four N-glycosylation sites in O.niloticus IgM heavy chain, namely, the Asn-315 site in the CH2 domain, the Asn-338 site in the CH3 domain, and the Asn-509 and Asn-551 sites in the CH4 domain, All of the four residues were efficiently N-glycosylated.
32887075	5	44	gly	N-glycosylated	1015:1028	arg1	the four residues			the four residues						residues	The results revealed that there are four N-glycosylation sites in O.niloticus IgM heavy chain, namely, the Asn-315 site in the CH2 domain, the Asn-338 site in the CH3 domain, and the Asn-509 and Asn-551 sites in the CH4 domain, All of the four residues were efficiently N-glycosylated.
29756380	1	28	gly	serine	161:166	arg1	residues			residues						serine and threonine residues	O-Linked glycosylation of serine and threonine residues of nucleocytoplasmic proteins with N-acetylglucosamine (O-GlcNAc) residues is catalyzed by O-GlcNAc transferase (OGT).
29756380	1	37	gly	glycosylation	144:156	arg1	serine			serine and threonine residues						serine and threonine residues	O-Linked glycosylation of serine and threonine residues of nucleocytoplasmic proteins with N-acetylglucosamine (O-GlcNAc) residues is catalyzed by O-GlcNAc transferase (OGT).
34089345	1	16	gly	glycoprotein	295:306	arg1	the endogenous glycan and glycoprotein ligands				the endogenous glycan and glycoprotein ligands						The macrophage mannose receptor (CD206, MR) is an endocytic lectin receptor which plays an important role in homeostasis and innate immunity, however, the endogenous glycan and glycoprotein ligands recognized by its C-type lectin domains (CTLD) have not been well studied.
32699088	5	24	gly	glycosylation	944:956	arg2	head glycosylation sites			head glycosylation sites						sites	In contrast, variable sites exist in H3N2 IAVs of human and swine origin, where the number of head glycosylation sites has mainly increased over time.
28100911	1	56	gly	glycoprotein	261:272	arg1	small integrin-binding ligand N-linked glycoprotein	small integrin-binding ligand N-linked glycoprotein				Fterm		glycoprotein			A recent genome-wide association study (GWAS) for dental caries nominated the chromosomal region 4q21 near ABCG2, PKD2 and the SIBLING (small integrin-binding ligand N-linked glycoprotein) gene family.
28100911	1	56	gly	glycoprotein	261:272	arg1	ABCG2	ABCG2				PUBTATOR		ABCG2	9429		A recent genome-wide association study (GWAS) for dental caries nominated the chromosomal region 4q21 near ABCG2, PKD2 and the SIBLING (small integrin-binding ligand N-linked glycoprotein) gene family.
29672582	10	71	gly	glycosylation	1731:1743	arg1	IgG4 N-glycans				IgG4 N-glycans						Furthermore, there were no significant differences in the glycosylation of IgG4 N-glycans between patients with and without individual organ involvement of IgG4RD.
28637198	2	59	gly	glycoproteins	509:521	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Among them, there are the HIV-1 reverse transcriptase (RT)-associated ribonuclease H (RNase H) function and the cellular α-glucosidase, involved in the control mechanisms of N-linked glycoproteins formation in the endoplasmic reticulum.
30285435	1	1	gly	glycosylation	87:99	arg1	nuclear and cytoplasmic proteins	nuclear and cytoplasmic proteins				Fterm		proteins			Reversible glycosylation of nuclear and cytoplasmic proteins is an important regulatory mechanism across metazoans.
30487799	4	24	gly	occupied	690:697	arg2	Asn-565			Asn-565				Grass Carp Serum IgM	Q15327	Asn-565	Among the four glycosylation sites, the Asn-262, Asn-303, and Asn-426 residues were efficiently glycosylated, while Asn-565 at the C-terminal tailpiece was incompletely occupied.
30487799	4	51	gly	glycosylated	617:628	arg1	the Asn-262, Asn-303, and Asn-426 residues			the Asn-262, Asn-303, and Asn-426 residues				Grass Carp Serum IgM	Q15327	Asn-262, Asn-303, and Asn-426 residues	Among the four glycosylation sites, the Asn-262, Asn-303, and Asn-426 residues were efficiently glycosylated, while Asn-565 at the C-terminal tailpiece was incompletely occupied.
30487799	4	67	gly	glycosylation	536:548	arg2	the four glycosylation sites			the four glycosylation sites						sites	Among the four glycosylation sites, the Asn-262, Asn-303, and Asn-426 residues were efficiently glycosylated, while Asn-565 at the C-terminal tailpiece was incompletely occupied.
29755357	7	11	gly	N-glycosylation	1343:1357	arg2	the N-glycosylation site			the N-glycosylation site						site	Furthermore, a hydrophobic cluster analysis revealed that the N-glycosylation site is embedded in a highly hydrophobic evolutionarily conserved surrounding.
31336250	4	25	gly	aglycosylated	918:930	arg1	aglycosylated Fc regions			aglycosylated Fc regions						regions	In this work, we constructed a huge library of Fc variants with combinations of 25 beneficial mutations that were previously identified to improve binding of glycosylated or aglycosylated Fc regions to human FcγRs in previous studies.
29920571	0	23	gly	glycopeptides	120:132	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	Hydrophilic tripeptide-functionalized magnetic metal-organic frameworks for the highly efficient enrichment of N-linked glycopeptides.
30991145	2	54	gly	glycoprotein	422:433	arg1	The G protein	The G protein				OGER		G protein			The G protein, which harbors the major antigenic determinants of IHNV, is an envelope glycoprotein that plays an important role in both pathogenicity and immunogenicity of IHNV.
30991145	2	54	gly	glycoprotein	422:433	arg1	an envelope glycoprotein	an envelope glycoprotein				Fterm		glycoprotein			The G protein, which harbors the major antigenic determinants of IHNV, is an envelope glycoprotein that plays an important role in both pathogenicity and immunogenicity of IHNV.
34343291	3	48	gly	receptor	554:561	arg1	N-glycans	insulin receptor			N-glycans	PUBTATOR		insulin receptor	3643		One such example is the development of insulin resistance, which has been attributed to the removal of sialic acid residues from N-glycans of insulin receptor (IR) from various experimental studies.
30712568	2	56	gly	glycopeptides	389:401	arg2	glycopeptides			glycopeptides						glycopeptides	However, a tedious synthesis and low specificity for glycopeptides have restricted its application.
30327117	8	48	gly	peptide	1491:1497	arg1	O-GlcNAc-CREB				O-GlcNAc-CREB						The accurate detection range of O-GlcNAc was 4.65 × 10-12 to 4.65 × 10-7 M which was obtained by quantifying the amount of a standard O-GlcNAcylated peptide (O-GlcNAc-CREB), and the detection limit is 4.65 × 10-13 M.
30327117	8	48	gly	peptide	1491:1497	arg1	a standard O-GlcNAcylated peptide				a standard O-GlcNAcylated peptide						The accurate detection range of O-GlcNAc was 4.65 × 10-12 to 4.65 × 10-7 M which was obtained by quantifying the amount of a standard O-GlcNAcylated peptide (O-GlcNAc-CREB), and the detection limit is 4.65 × 10-13 M.
32326134	7	25	gly	O-glycosylation	1626:1640	arg2	O-glycosylation sites			O-glycosylation sites						sites	This is the first study to characterize O-glycans and identify O-glycosylation sites in BSM.
31563509	6	61	gly	glycoprotein	1321:1332	arg1	N-linked glycoprotein biosynthesis	N-linked glycoprotein biosynthesis				Fterm		glycoprotein			Herein, we present a multipronged biochemical and biophysical study of PglC, a monotopic phosphoglycosyl transferase that catalyzes the first membrane-committed step in N-linked glycoprotein biosynthesis in Campylobacter jejuni.
29070692	1	63	gly	glycoproteins	313:325	arg1	two viral surface glycoproteins	two viral surface glycoproteins				Fterm		glycoproteins			Heartland virus (HRTV) is an emerging human pathogen that belongs to the newly defined family Phenuiviridae, order Bunyavirales Gn and Gc are two viral surface glycoproteins encoded by the M segment and are required for early events during infection.
29070692	1	63	gly	glycoproteins	313:325	arg1	Gc	Gc				Cterm		Gc			Heartland virus (HRTV) is an emerging human pathogen that belongs to the newly defined family Phenuiviridae, order Bunyavirales Gn and Gc are two viral surface glycoproteins encoded by the M segment and are required for early events during infection.
31604106	4	129	gly	O-glycosylation	575:589	arg1	recombinant hCG protein expression	hCG protein				PUBTATOR		hCG protein	93659		The effects of O-glycosylation on recombinant hCG protein expression were assessed by adding O-glycan precursors and overexpressing and knocking down key regulatory genes of O-glycan precursor synthesis and O-glycan sugar chain synthesis or hydrolases.
31727010	1	71	gly	glycoproteins	319:331	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			BACKGROUND PMM2-CDG, is the most common N-linked glycosylation disorder and subtype among all CDG syndromes, which are a series of genetic disorders involving the synthesis and attachment of glycoproteins and glycolipid glycans.
30622981	4	14	gly	glycosylation	800:812	arg2	two glycosylation sequons			two glycosylation sequons							Previously, we demonstrated that an ExoA glycoconjugate with two glycosylation sequons was capable of providing significant protection to mice against a challenge with a low-virulence strain of F. tularensis.
27728760	4	0	gly	glycoproteins	805:817	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			As with the lysate proteomes examined previously, the exosome surface N-glycoproteins are primarily a subset of the glycoproteins on the surface of the suppressor cells that released them, with related functions and related potential as therapeutic targets.
27728760	4	3	gly	N-glycoproteins	759:773	arg1	the exosome surface N-glycoproteins	the exosome surface N-glycoproteins				Fterm		N-glycoproteins			As with the lysate proteomes examined previously, the exosome surface N-glycoproteins are primarily a subset of the glycoproteins on the surface of the suppressor cells that released them, with related functions and related potential as therapeutic targets.
35518855	0	56	gly	core-fucosylated	24:39	arg1	core-fucosylated glycans				core-fucosylated glycans						Increased expression of core-fucosylated glycans in human lung squamous cell carcinoma.
31600726	2	31	gly	attached	240:247	arg1	the Asn7 and Asn24 residues AND N-linked glycans			the Asn7 and Asn24 residues	N-linked glycans			FSH	14308	Asn7 and Asn24 residues	N-linked glycans are co-translationally attached to the Asn7 and Asn24 residues on the FSHβ subunit.
30105014	1	57	gly	domain	474:479	arg1	its carbohydrate recognition domain (CRD) binding				its carbohydrate recognition domain (CRD) binding						SP-D can inhibit hemagglutination and infectivity of IAV, in addition to reducing neuraminidase (NA) activity via its carbohydrate recognition domain (CRD) binding to carbohydrate patterns (N-linked mannosylated) on NA and hemagglutinin (HA) of IAV.
30105014	1	85	gly	binding	487:493	arg1	hemagglutinin	hemagglutinin			binding	Fterm		hemagglutinin			SP-D can inhibit hemagglutination and infectivity of IAV, in addition to reducing neuraminidase (NA) activity via its carbohydrate recognition domain (CRD) binding to carbohydrate patterns (N-linked mannosylated) on NA and hemagglutinin (HA) of IAV.
33026663	5	5	gly	glycoforms	567:576	arg1	invertase glycoforms	invertase glycoforms				Fterm		invertase			A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionated by anion-exchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1-EINV3).
31783892	2	3	gly	glycosylation	193:205	arg1	PD-1	PD-1				OGER		PD-1	Q15116		Reducing N-linked glycosylation of PD-1 may decrease PD-1 expression and relieve its inhibitory effects on CAR-T cells.
29030255	9	20	gly	residues	1151:1158	arg1	two cysteine residues			two cysteine residues						cysteine residues	Three potential N-linked glycosylation sites and two cysteine residues (Cys-28 and Cys-209) that are likely to form one disulfide bond were present in pufferfish CA VI.
29030255	9	20	gly	residues	1151:1158	arg1	Cys-28	CA VI		Cys-28 and Cys-209				CA VI	765	Cys-28 and Cys-209	Three potential N-linked glycosylation sites and two cysteine residues (Cys-28 and Cys-209) that are likely to form one disulfide bond were present in pufferfish CA VI.
29030255	9	113	gly	glycosylation	1114:1126	arg1	pufferfish CA VI	CA VI		sites		PUBTATOR		CA VI	765	sites	Three potential N-linked glycosylation sites and two cysteine residues (Cys-28 and Cys-209) that are likely to form one disulfide bond were present in pufferfish CA VI.
31079966	3	46	gly	glycopeptide	678:689	arg2	synthetic glycopeptide			synthetic glycopeptide						glycopeptide	The comparative NMR analysis of synthetic glycopeptide having different length of the O-mannosylated glycans revealed a conformational change of the peptide backbone along with core M1 disaccharide formation.
31079966	3	72	gly	O-mannosylated	722:735	arg1	the O-mannosylated glycans				the O-mannosylated glycans						The comparative NMR analysis of synthetic glycopeptide having different length of the O-mannosylated glycans revealed a conformational change of the peptide backbone along with core M1 disaccharide formation.
34869209	2	56	gly	O-glycosites	294:305	arg2	O-glycosites			O-glycosites						O-glycosites	It is also O-glycosylated, although the number of O-glycosites is less defined.
33581334	5	34	gly	O-glycoprotein	687:700	arg1	the largest O-glycoprotein	the largest O-glycoprotein				Fterm		O-glycoprotein			OGP contains 9354 O-glycosylation sites and 11,633 site-specific O-glycans mapping to 2133 O-glycoproteins, and it is the largest O-glycoprotein repository thus far.
33581334	5	44	gly	O-glycosylation	575:589	arg2	9354 O-glycosylation sites			9354 O-glycosylation sites						sites	OGP contains 9354 O-glycosylation sites and 11,633 site-specific O-glycans mapping to 2133 O-glycoproteins, and it is the largest O-glycoprotein repository thus far.
33581334	5	75	gly	O-glycoproteins	648:662	arg1	2133 O-glycoproteins	2133 O-glycoproteins				Fterm		O-glycoproteins			OGP contains 9354 O-glycosylation sites and 11,633 site-specific O-glycans mapping to 2133 O-glycoproteins, and it is the largest O-glycoprotein repository thus far.
33581334	5	15	gly	contains	561:568	arg1	OGP AND 11,633 site-specific O-glycans	OGP		9354 O-glycosylation sites	11,633 site-specific O-glycans	PUBTATOR	Site	OGP	5016	sites	OGP contains 9354 O-glycosylation sites and 11,633 site-specific O-glycans mapping to 2133 O-glycoproteins, and it is the largest O-glycoprotein repository thus far.
27388694	1	12	gly	glycoproteins	219:231	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			PURPOSE The cytosolic enzyme N-glycanase 1, encoded by NGLY1, catalyzes cleavage of the β-aspartyl glycosylamine bond of N-linked glycoproteins, releasing intact N-glycans from proteins bound for degradation.
30036038	8	56	gly	glycoproteins	1311:1323	arg1	O-linked glycoproteins	O-linked glycoproteins				Fterm		glycoproteins			The strategy is particularly significant for the recognition of O-glycans, because enzymes that can release O-glycans from O-linked glycoproteins are lacking.
31665542	0	33	gly	glycosylation	9:21	arg1	an S-layer protein	an S-layer protein				Fterm		protein			Enhanced glycosylation of an S-layer protein enables a psychrophilic methanogenic archaeon to adapt to elevated temperatures in abundant substrates.
32965048	5	112	gly	N-glycopeptide	1196:1209	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	METHODS We performed trypsin digestion, Zwitterionic Hydrophilic Interaction chromatography (ZIC-HILIC) enrichment, C18-RPLC/nano-ESI-MS/MS using HCD with stepped normalized collisional energies, and GPSeeker database search for a comprehensive site- and structure-specific N-glycoproteomics characterization of the human urinary N-glycoproteome at the intact N-glycopeptide level.
34106099	7	69	gly	sialylated	1130:1139	arg1	intact sialylated N-linked glycopeptides			intact sialylated N-linked glycopeptides						glycopeptides	Characterization of intact sialylated N-linked glycopeptides enriched by titanium dioxide chromatography revealed more than 85% selectivity of sialylated species and the presence of NeuGc on active zone proteins.
34106099	7	74	gly	glycopeptides	1150:1162	arg2	intact sialylated N-linked glycopeptides			intact sialylated N-linked glycopeptides						glycopeptides	Characterization of intact sialylated N-linked glycopeptides enriched by titanium dioxide chromatography revealed more than 85% selectivity of sialylated species and the presence of NeuGc on active zone proteins.
34106099	7	81	gly	presence	1273:1280	arg1	active zone proteins AND NeuGc	active zone proteins			NeuGc	Fterm		proteins			Characterization of intact sialylated N-linked glycopeptides enriched by titanium dioxide chromatography revealed more than 85% selectivity of sialylated species and the presence of NeuGc on active zone proteins.
29597345	7	20	gly	Domain	1433:1438	arg1	the EGF Domain Specific O-Linked N-Acetylglucosamine				the EGF Domain Specific O-Linked N-Acetylglucosamine						We provided evidence that residential PM10 exposure in early life affects methylation of the CpG cg21785536 located on the EGF Domain Specific O-Linked N-Acetylglucosamine Transferase gene.
31129958	4	25	gly	glycopeptide	674:685	arg2	an on-demand decoy glycopeptide generator			an on-demand decoy glycopeptide generator						glycopeptide	To address this need, we have designed DecoyDeveloper, an on-demand decoy glycopeptide generator that can produce a high volume of decoys with low mass differences.
30205382	9	78	gly	JAK2-Tyr705STAT3-VEGF	1647:1667	arg1	the JAK2-Tyr705STAT3-VEGF pathway	JAK2			the JAK2-Tyr705STAT3-VEGF pathway	PUBTATOR		JAK2	3717		Next, we observed that overexpression of O-GlcNAcylation could relieve human RVEC apoptosis related to the JAK2-Tyr705STAT3-VEGF pathway.
32469948	7	40	gly	β-1,2-N-acetylglucosaminyltransferase	1029:1065	arg1	mannosylation	N-acetylglucosaminyltransferase			mannosylation	OGER		N-acetylglucosaminyltransferase	Q8N0V5		In addition, we knocked in the MdsI (α-1,2-mannosidase) gene to reduce mannosylation and the GnTI (β-1,2-N-acetylglucosaminyltransferase I) and GnTII genes to produce human N-glycan structures.
30962950	3	24	gly	glycoproteins	542:554	arg1	candidate glycoproteins	candidate glycoproteins				Fterm		glycoproteins			The prognostic significance of candidate glycoproteins was then validated in 193 HCC tissues using immunohistochemical staining.
30262754	7	42	gly	O-glycosylation	1511:1525	arg1	proteins	proteins				Fterm		proteins			Therefore, GalNAc-T3 expression seems related to the quality of the spermatozoa, and we propose that reduced expression of GalNAc-T3 may lead to impaired O-glycosylation of proteins and thereby abnormal maturation and reduced functionality of the spermatozoa.
30158294	8	95	gly	N-glycosylation	1108:1122	arg1	SERINC5	SERINC5		site		PUBTATOR		SERINC5	256987	site	Our results demonstrate that N294 is the major site of N-glycosylation in SERINC5.
30158294	8	95	gly	N-glycosylation	1108:1122	arg1	SERINC5	SERINC5		N294		PUBTATOR		SERINC5	256987	N294	Our results demonstrate that N294 is the major site of N-glycosylation in SERINC5.
33806155	5	75	gly	O-glycosites	907:918	arg2	25 O-glycosites			25 O-glycosites						O-glycosites	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.
34523671	2	87	gly	glycosylation	546:558	arg2	the site			the site						site	Substrate preference across different isoforms of this enzyme is influenced by isoform-specific amino acid sequences at the site of glycosylation, which we have exploited to engineer production of Core 1 glycan structures in bacteria on human therapeutic proteins.
31101650	5	92	gly	under-glycosylated	1071:1088	arg1	furin	furin				PUBTATOR		furin	5045		Providing cDNA for Golgi-resident proprotein convertase subtilisin/kexin type 5a (PCSK5a) and furin cDNA to wild-type and mutant cells produced under-glycosylated forms of PCSK5a, but not furin, in cells lacking STT3A.
28067406	9	40	gly	N-glycosylation	1562:1576	arg2	an N-glycosylation motif			an N-glycosylation motif						motif	In silico analysis revealed that Ninj1 is highly conserved in vertebrates and that the conserved sequence contains an N-glycosylation motif and cis-interacting intracellular region, which participate in Ninj1 homomer assembly.
34864596	1	28	gly	glycoprotein	122:133	arg1	a glycoprotein based biotherapeutics	a glycoprotein based biotherapeutics				Fterm		glycoprotein			Omalizumab, a glycoprotein based biotherapeutics, is one of the most frequently used targeted antibody biopharmaceutical to reduce asthma exacerbations, improve lung function and reduce oral corticosteroid use.
31831633	8	17	gly	glycoform	1356:1364	arg1	the altered MUC1-sTn glycoform	the altered MUC1-sTn glycoform				OGER		MUC1	P15941		Immunostaining of ulcerative colitis and CACC tissue samples confirmed the elevated number of M2-like macrophages as well as high expression of ST6GALNAC1 and the altered MUC1-sTn glycoform on colon cells.
30029404	2	13	gly	glycopeptides	479:491	arg2	glycopeptides			glycopeptides						glycopeptides	Based on the trap columns, a novel on-column enrichment method toward glycopeptides was developed as a sample pretreatment step prior to matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) or nano-LC-MS/MS analysis.
31516090	7	41	gly	positions	891:899	arg1	positions s116, s123, s130, and s131 + s133			positions s116, s123, s130, and s131 + s133						positions	The first group included mutations creating new N-linked glycosylation sites at positions s116, s123, s130, and s131 + s133 or removing the existing one at s146.
31516090	7	56	gly	glycosylation	868:880	arg2	new N-linked glycosylation sites			new N-linked glycosylation sites						sites	The first group included mutations creating new N-linked glycosylation sites at positions s116, s123, s130, and s131 + s133 or removing the existing one at s146.
29093093	10	93	gly	glycoproteins	2388:2400	arg1	most other characterized coronavirus S glycoproteins	most other characterized coronavirus S glycoproteins				Fterm		glycoproteins			PDCoV S is largely protease resistant, which distinguishes it from most other characterized coronavirus S glycoproteins and suggests that enteric coronaviruses have evolved to fine-tune fusion activation in the protease-rich environment of the small intestine of infected hosts.
32452486	2	44	gly	glycans	245:251	arg1	proteins	proteins			glycans	Fterm		proteins			Therefore rapid and sensitive differentiation of glycans on proteins is highly desirable.
27666939	4	16	gly	afucosylated	622:633	arg1	fully afucosylated glycans				fully afucosylated glycans						A commonly used and efficient approach has been knocking out the FUT8 gene of the Chinese hamster ovary (CHO) host cells, which results in expression of antibody molecules with fully afucosylated glycans.
31306674	1	17	gly	glycoproteins	236:248	arg1	viral N-linked glycoproteins	viral N-linked glycoproteins				Cterm		N-linked glycoproteins	3458		The antiviral mechanism of action of iminosugars against many enveloped viruses is hypothesized to be a consequence of misfolding of viral N-linked glycoproteins through inhibition of host endoplasmic reticulum α-glucosidase enzymes.
31879931	7	59	gly	glycopeptide	1256:1267	arg2	two HIV-1 V3 glycopeptide antigens			two HIV-1 V3 glycopeptide antigens						glycopeptide	In this chapter, we provide detailed procedures of this chemoenzymatic method by demonstrating the synthesis of two HIV-1 V3 glycopeptide antigens carrying a high-mannose-type and a complex-type N-glycan, respectively.
32784866	3	45	gly	glycosylation	562:574	arg1	native fibrinogen	native fibrinogen				PUBTATOR		fibrinogen	2244		Taking into consideration that fibrinogen mediates these processes, we isolated fibrinogen from the plasma from patients with ESRD on peritoneal dialysis (ESRD-PD), and examined glycosylation of native fibrinogen and its subunits by lectin-based microarray and lectin blotting.
32784866	3	45	gly	glycosylation	562:574	arg1	its subunits	its subunits				OGER		subunits	P17813		Taking into consideration that fibrinogen mediates these processes, we isolated fibrinogen from the plasma from patients with ESRD on peritoneal dialysis (ESRD-PD), and examined glycosylation of native fibrinogen and its subunits by lectin-based microarray and lectin blotting.
30514763	3	60	gly	glycosylated	593:604	arg1	highly glycosylated thrombospondin repeat (TSR) domains			highly glycosylated thrombospondin repeat (TSR) domains							Here we demonstrate that micronemal protein 2 (MIC2), a motility-associated adhesin of T. gondii, has highly glycosylated thrombospondin repeat (TSR) domains.
30514763	3	23	gly	has	582:584	arg1	micronemal protein 2 AND highly glycosylated thrombospondin repeat (TSR) domains	micronemal protein 2			highly glycosylated thrombospondin repeat (TSR) domains	Fterm		protein 2			Here we demonstrate that micronemal protein 2 (MIC2), a motility-associated adhesin of T. gondii, has highly glycosylated thrombospondin repeat (TSR) domains.
30514763	3	23	gly	has	582:584	arg1	MIC2 AND highly glycosylated thrombospondin repeat (TSR) domains	MIC2			highly glycosylated thrombospondin repeat (TSR) domains	OGER		2 (MIC2	P14209		Here we demonstrate that micronemal protein 2 (MIC2), a motility-associated adhesin of T. gondii, has highly glycosylated thrombospondin repeat (TSR) domains.
30514763	3	23	gly	has	582:584	arg1	a motility-associated adhesin AND highly glycosylated thrombospondin repeat (TSR) domains	a motility-associated adhesin			highly glycosylated thrombospondin repeat (TSR) domains	Fterm		adhesin			Here we demonstrate that micronemal protein 2 (MIC2), a motility-associated adhesin of T. gondii, has highly glycosylated thrombospondin repeat (TSR) domains.
29780502	4	48	gly	O-mannosylated	784:797	arg1	a model IDP	a model IDP				OGER		IDP	O75874		Here, we synthesized variants of a model IDP, specifically a natively O-mannosylated linker from a fungal enzyme, with α-O-linked mannose, glucose, and galactose moieties, along with a non-glycosylated linker.
34551980	4	32	gly	neoglycoproteins	913:928	arg1	The resulting neoglycoproteins	The resulting neoglycoproteins				Fterm		neoglycoproteins			The resulting neoglycoproteins can be readily subjected to available high-throughput assays, making it possible to systematically investigate the structural and functional consequences of glycan conjugation along a protein backbone.
29337188	6	15	gly	glycoprotein	1163:1174	arg1	N-linked glycoprotein (SIBLING) family	N-linked glycoprotein (SIBLING) family				Fterm		glycoprotein			Fam20c deficiency also significantly reduced the expression of Osterix, Runx2, type I Collagen a 1 (Col1a1), Alkaline phosphatase (Alpl) and the members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family, but increased Fgf23 expression.
27690717	6	2	gly	N-glycosylation	1230:1244	arg1	β2 subunits	β2 subunits				PUBTATOR		2 subunits	23545		Our results suggest that N-glycosylation of β2 subunits plays crucial roles in imparting functional heterogeneity of BK channels, and is potentially involved in the pathological phenotypes of carbohydrate metabolic diseases.
27690717	6	9	gly	heterogeneity	1305:1317	arg1	BK channels	BK channels				Fterm		channels			Our results suggest that N-glycosylation of β2 subunits plays crucial roles in imparting functional heterogeneity of BK channels, and is potentially involved in the pathological phenotypes of carbohydrate metabolic diseases.
34864596	2	39	gly	glycoprotein	368:379	arg1	such glycoprotein drugs	such glycoprotein drugs				Fterm		glycoprotein			The effector function and clearance time of such glycoprotein drugs is affected by their N-glycosylation, that defines the required administration frequency to improve the quality of life in appropriately selected patients.
31059811	6	69	gly	glycosylation	911:923	arg2	12 potential N-linked glycosylation sites			12 potential N-linked glycosylation sites						sites	In addition, 12 potential N-linked glycosylation sites were present in the ectodomain to influence protein trafficking, surface presentation and ligand recognition.
31059811	6	69	gly	glycosylation	911:923	arg1	the ectodomain to influence protein trafficking, surface presentation and ligand recognition			the ectodomain to influence protein trafficking, surface presentation and ligand recognition						ectodomain	In addition, 12 potential N-linked glycosylation sites were present in the ectodomain to influence protein trafficking, surface presentation and ligand recognition.
32269229	4	7	gly	N-glycosites	931:942	arg2	N-glycosites			N-glycosites						N-glycosites	We then investigated the basis of mutual exclusivity between disorder and N-glycosites on the basis of amino acid distribution: when compared with control ordered residue stretches without any N-glycosites, residue neighborhoods surrounding N-glycosites showed a depletion of bulky, hydrophobic and disorder-promoting amino acids and an enrichment for flexible and accessible residues that are frequently found in coiled structures.
32269229	4	48	gly	N-glycosites	883:894	arg2	any N-glycosites			any N-glycosites						N-glycosites	We then investigated the basis of mutual exclusivity between disorder and N-glycosites on the basis of amino acid distribution: when compared with control ordered residue stretches without any N-glycosites, residue neighborhoods surrounding N-glycosites showed a depletion of bulky, hydrophobic and disorder-promoting amino acids and an enrichment for flexible and accessible residues that are frequently found in coiled structures.
32269229	4	59	gly	N-glycosites	764:775	arg2	N-glycosites			N-glycosites						N-glycosites	We then investigated the basis of mutual exclusivity between disorder and N-glycosites on the basis of amino acid distribution: when compared with control ordered residue stretches without any N-glycosites, residue neighborhoods surrounding N-glycosites showed a depletion of bulky, hydrophobic and disorder-promoting amino acids and an enrichment for flexible and accessible residues that are frequently found in coiled structures.
31067000	4	54	gly	presence	630:637	arg2	IgG ACPA V domains AND glycans			IgG ACPA V domains	glycans					domains	As ample evidence indicates that T cells drive the maturation of the ACPA response prior to arthritis onset, we undertook this study to investigate whether the presence of glycans in IgG ACPA V domains predicts the transition from predisease autoimmunity to overt RA.
29609090	0	61	gly	glycopeptides	67:79	arg1	relaxed nucleotide-activated sugar donor selectivity			glycopeptides	relaxed nucleotide-activated sugar donor selectivity					glycopeptides	N-Glycosyltransferase from Aggregatibacter aphrophilus synthesizes glycopeptides with relaxed nucleotide-activated sugar donor selectivity.
30130254	8	38	gly	modification	1129:1140	arg1	intracellular proteins	proteins			modification	Fterm		proteins			To explore the mechanism, we investigated protein glycosylation downstream of HBP and found elevated levels of O-linked β-N-acetylglucosamine (O-GlcNAcylation) posttranslational modification on intracellular proteins.
31275257	9	26	gly	sialylated	1400:1409	arg1	several mucin motifs			several mucin motifs						motifs	In total, the structural modification of several mucin motifs, sialylated mucin ones in particular, was evidenced for nine clones.
33525794	0	52	gly	glycoprotein	25:36	arg1	glycoprotein biosynthesis	glycoprotein biosynthesis				Fterm		glycoprotein			Metabolic engineering of glycoprotein biosynthesis in bacteria.
30991145	8	11	gly	glycosylation	2145:2157	arg1	asparagine 438			asparagine 438				G protein		asparagine 438	This mutant also elicited specific anti-IHNV IgM production earlier than other mutants, suggesting that glycosylation of asparagine 438 may be important for viral immune escape.
30178920	2	26	gly	glycopeptides	640:652	arg2	glycopeptides			glycopeptides						glycopeptides	The polymer monolithic material is used for glycopeptides enrichment coupled with MALDI-TOF MS. By taking advantage of cobalt phthalocyanine including hydrogen bonds between isoindole subunits of phthalocyanine and glycans, coordination interaction between cobalt and glycopeptides, the monolithic material is successfully applied to the enrichment of glycopeptides efficiently and selectively.
30178920	2	36	gly	glycopeptides	556:568	arg2	glycopeptides			glycopeptides						glycopeptides	The polymer monolithic material is used for glycopeptides enrichment coupled with MALDI-TOF MS. By taking advantage of cobalt phthalocyanine including hydrogen bonds between isoindole subunits of phthalocyanine and glycans, coordination interaction between cobalt and glycopeptides, the monolithic material is successfully applied to the enrichment of glycopeptides efficiently and selectively.
30178920	2	42	gly	glycopeptides	332:344	arg2	glycopeptides enrichment			glycopeptides enrichment						glycopeptides	The polymer monolithic material is used for glycopeptides enrichment coupled with MALDI-TOF MS. By taking advantage of cobalt phthalocyanine including hydrogen bonds between isoindole subunits of phthalocyanine and glycans, coordination interaction between cobalt and glycopeptides, the monolithic material is successfully applied to the enrichment of glycopeptides efficiently and selectively.
29717387	4	6	gly	glycosylation	466:478	arg2	two N-linked glycosylation sites			two N-linked glycosylation sites						sites	In this study, the N-glycosylation of PPARγ, as well as two N-linked glycosylation sites in its DNA binding domain (DBD), was identified.
29717387	4	7	gly	N-glycosylation	416:430	arg1	two N-linked glycosylation sites	PPAR		sites		PUBTATOR		PPAR	5468	sites	In this study, the N-glycosylation of PPARγ, as well as two N-linked glycosylation sites in its DNA binding domain (DBD), was identified.
29717387	4	16	gly	N-glycosylation	416:430	arg1	its DNA binding domain	PPAR		domain		PUBTATOR		PPAR	5468	domain	In this study, the N-glycosylation of PPARγ, as well as two N-linked glycosylation sites in its DNA binding domain (DBD), was identified.
30069741	3	1	gly	proteins	531:538	arg1	authentic or at least compatible glycan structures	proteins			authentic or at least compatible glycan structures	Fterm		proteins			The glyco-engineering of plant-based expression systems began as a strategy to eliminate plant-like glycans and produce human proteins with authentic or at least compatible glycan structures.
32168410	3	21	gly	linked	373:378	arg1	FXIII-B AND the glycan structures	FXIII-B			the glycan structures	PUBTATOR		FXIII-B	2165		OBJECTIVE To reveal the glycan structures linked to FXIII-B, to design a method for deglycosylating the native protein, to find out if deglycosylation influences the dimeric structure of FXIII-B and its clearance from the circulation.
33177111	10	66	gly	sialylation	1614:1624	arg1	CD55	CD55				OGER		CD55	P08174		These data demonstrated that ST3GAL1-mediated O-linked sialylation of CD55 acts like an immune checkpoint molecule for cancer cells to evade immune attack and that inhibition of ST3GAL1 is a potential strategy to block CD55-mediated immune evasion.
33177111	10	88	gly	CD55	1629:1632	arg1	ST3GAL1-mediated O-linked sialylation	CD55			ST3GAL1-mediated O-linked sialylation	OGER		CD55	P08174		These data demonstrated that ST3GAL1-mediated O-linked sialylation of CD55 acts like an immune checkpoint molecule for cancer cells to evade immune attack and that inhibition of ST3GAL1 is a potential strategy to block CD55-mediated immune evasion.
33947960	2	25	gly	glycosylation	522:534	arg1	the oncogenic cell surface receptor tyrosine kinase (RTK) ErbB2	the oncogenic cell surface receptor tyrosine kinase (RTK) ErbB2				PUBTATOR		ErbB2	2064		Trastuzumab's target epitope is localized within the extracellular domain of the oncogenic cell surface receptor tyrosine kinase (RTK) ErbB2, which is known to undergo extensive N-linked glycosylation.
31256377	8	45	gly	semiquantitative	1293:1308	arg1	site-specific			site-specific						site-specific	In this chapter, we describe quantitative analysis of collagen glycosylation by high-performance liquid chromatography (HPLC) and semiquantitative, site-specific analysis by HPLC-tandem mass spectrometry.
30713024	0	17	gly	OGT-tolerated	16:28	arg1	Ac4GlcNAcF3	OGT			Ac4GlcNAcF3	PUBTATOR		OGT	108155		Ac4GlcNAcF3, an OGT-tolerated but OGA-resistant regulator for O-GlcNAcylation.
30713024	0	27	gly	OGA-resistant	34:46	arg1	Ac4GlcNAcF3	OGA			Ac4GlcNAcF3	PUBTATOR		OGA	76055		Ac4GlcNAcF3, an OGT-tolerated but OGA-resistant regulator for O-GlcNAcylation.
31029427	8	6	gly	sites	1339:1343	arg1	FOXA1	FOXA1			sites	PUBTATOR		FOXA1	3169		Through an ESI-MS assay, S354 and S355 were identified as probable O-GalNAcylation sites on FOXA1.
30850477	6	47	gly	N-glycosylation	675:689	arg2	N-glycosylation sites			N-glycosylation sites						sites	We analyzed the distribution and acquisition of N-glycosylation sites within Ab V regions of peripheral blood and bone marrow B cells of 12 healthy individuals, eight myasthenia gravis patients, and six systemic lupus erythematosus patients, obtained by next-generation sequencing.
30814666	5	53	gly	glycosylated	701:712	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			This data set represents the highest number of glycosylated proteins identified in Mtb to date.
30697857	0	29	gly	N-glycoproteins	0:14	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			N-glycoproteins exhibit a positive expression level-evolutionary rate correlation.
29237830	4	12	gly	glycosylation	520:532	arg1	DG	DG				Cterm		DG	1605		Interestingly, functional glycosylation of DG does not always correlate with viral tropism observed in vivo The broadly expressed phosphatidylserine (PS) receptors Axl and Tyro3 were recently identified as alternative LASV receptor candidates.
29513221	5	32	gly	glycosylation	942:954	arg2	specific vimentin glycosylation sites			specific vimentin glycosylation sites						sites	In addition, we show that the intracellular pathogen Chlamydia trachomatis, which remodels the host IF cytoskeleton during infection, requires specific vimentin glycosylation sites and O-GlcNAc transferase activity to maintain its replicative niche.
29983388	2	5	gly	proteins	513:520	arg1	N-linked glycans	proteins			N-linked glycans	Fterm		proteins			We have found an unexplored mechanism, by which basic fibroblast growth factor (FGF2) induces expression of fucosyltransferase 8 (FUT8) to increase core fucosylations of N-linked glycans of membrane-associated proteins, including several integrin subunits.
29983388	2	15	gly	fucosylations	456:468	arg1	N-linked glycans				N-linked glycans						We have found an unexplored mechanism, by which basic fibroblast growth factor (FGF2) induces expression of fucosyltransferase 8 (FUT8) to increase core fucosylations of N-linked glycans of membrane-associated proteins, including several integrin subunits.
30709903	0	18	gly	O-glycosylated	120:133	arg1	O-glycosylated CREB	O-glycosylated CREB				PUBTATOR		CREB	12912		Iron down-regulates leptin by suppressing protein O-GlcNAc modification in adipocytes, resulting in decreased levels of O-glycosylated CREB.
34855283	4	49	gly	glycoproteins	728:740	arg1	894 glycoproteins	894 glycoproteins				Fterm		glycoproteins			By using our newly developed glycoproteomic methods and novel algorithm, termed 'StrucGP', a total of 486 N-glycan structures attached on 1235 glycosites were identified from 894 glycoproteins in ICC and HCC tumors.
34855283	4	54	gly	attached	675:682	arg2	486 N-glycan structures AND 1235 glycosites			1235 glycosites	486 N-glycan structures					glycosites	By using our newly developed glycoproteomic methods and novel algorithm, termed 'StrucGP', a total of 486 N-glycan structures attached on 1235 glycosites were identified from 894 glycoproteins in ICC and HCC tumors.
34855283	4	59	gly	glycosites	692:701	arg2	1235 glycosites			1235 glycosites						glycosites	By using our newly developed glycoproteomic methods and novel algorithm, termed 'StrucGP', a total of 486 N-glycan structures attached on 1235 glycosites were identified from 894 glycoproteins in ICC and HCC tumors.
34885895	3	17	gly	glycosylation	443:455	arg2	the glycosylation site			the glycosylation site						site	Most of the existing predictors for N-linked glycosylation utilize the information that the glycosylation site occurs at the N-X-[S/T] sequon, where X is any amino acid except proline.
29577901	7	4	gly	peptide	1195:1201	arg1	ncOGT	OGT			peptide	PUBTATOR		OGT	108155		Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain.
29577901	7	4	gly	peptide	1195:1201	arg1	the longest OGT isoform	OGT isoform			peptide	PUBTATOR		OGT isoform	8473		Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain.
29577901	7	4	gly	peptide	1195:1201	arg1	the intervening region			the intervening region	the intervening region		Site			region	Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain.
29577901	7	70	gly	sites	1039:1043	arg1	ncOGT	OGT			sites	PUBTATOR		OGT	108155		Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain.
29577901	7	70	gly	sites	1039:1043	arg1	the longest OGT isoform	OGT isoform			sites	PUBTATOR		OGT isoform	8473		Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain.
29577901	7	70	gly	sites	1039:1043	arg1	the intervening region			the intervening region	the intervening region		Site			region	Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain.
34128680	5	56	gly	NQ11	971:974	arg1	N(GlcNAc)SGSG-Q11	NQ11			N(GlcNAc)SGSG-Q11	Cterm		NQ11			Specifically, an NQ11 variant modified with N-linked N-acetylglucosamine, N(GlcNAc)SGSG-Q11 (GQ11), formed β-sheet nanofibers more slowly than NQ11 in deionized water (pH 5.8), which correlated to the tendency of GQ11 to form a combination of short fibrils and nonfibrillar aggregates, whereas NQ11 formed extended nanofibers.
33404348	3	43	gly	glycoprotein	552:563	arg1	glycoprotein biomarkers	glycoprotein biomarkers				Fterm		glycoprotein			In the past, cancer studies heavily relied on genomics and transcriptomics with relatively little exploration of the glycan alterations and glycoprotein biomarkers among individuals and populations.
32535395	12	51	gly	glycoprotein	1799:1810	arg1	glycoprotein production	glycoprotein production				Fterm		glycoprotein			The data strongly suggests that glycoprotein production by luminal epithelial cells is influenced by the presence of a conceptus.
29269413	4	40	gly	β1,4-galactosyltransferase	641:666	arg1	β1,4GalTV	1,4-galactosyltransferase V			β1,4GalTV	PUBTATOR		1,4-galactosyltransferase V	9334		We have previously reported that β1,4-galactosyltransferase V (β1,4GalTV), which galactosylates the GlcNAcβ1-6Man arm of the branched N-glycans, is highly expressed in glioma and promotes glioma cell growth in vitro and in vivo However, the mechanism by which β1,4GalTV stimulates glioma growth is unknown.
32890705	2	74	gly	branch	303:308	arg1	specific proteins	proteins			branch	Fterm		proteins			N-Glycans have a different number of N-acetylglucosamine (GlcNAc) branches in a protein-selective manner, and the β1,6-linked GlcNAc branch on specific proteins produced by N-acetylglucosaminyltransferase-V (GnT-V or MGAT5) promotes cancer malignancy.
34857845	4	67	gly	fucosylated	581:591	arg1	core fucosylated N-glycopeptides			core fucosylated N-glycopeptides						N-glycopeptides	Mass spectrometry efficiently identifies structures of fucosylated glycans or sites of core fucosylated N-glycopeptides but quantification of the glycopeptides remains less explored.
34857845	4	69	gly	fucosylated	544:554	arg1	fucosylated glycans				fucosylated glycans						Mass spectrometry efficiently identifies structures of fucosylated glycans or sites of core fucosylated N-glycopeptides but quantification of the glycopeptides remains less explored.
34857845	4	74	gly	glycopeptides	635:647	arg2	the glycopeptides			the glycopeptides						glycopeptides	Mass spectrometry efficiently identifies structures of fucosylated glycans or sites of core fucosylated N-glycopeptides but quantification of the glycopeptides remains less explored.
34857845	4	44	gly	N-glycopeptides	593:607	arg1	fucosylated glycans			N-glycopeptides	fucosylated glycans					N-glycopeptides	Mass spectrometry efficiently identifies structures of fucosylated glycans or sites of core fucosylated N-glycopeptides but quantification of the glycopeptides remains less explored.
31164864	8	42	gly	TxNIP	1322:1326	arg1	O-GlcNAcylation	TxNIP			O-GlcNAcylation	PUBTATOR		TxNIP	117514		Interestingly, expression and O-GlcNAcylation of TxNIP appeared to be increased in islets of diabetic rodents.
30834446	6	40	gly	glycoprotein	1059:1070	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			Like other rhabdoviruses, IHNV is characterized by an envelope consisting of trimers of a glycoprotein that display multiple N-linked oligosaccharides and play an integral role in viral infection by mediating the virus attachment and fusion.
36303733	6	26	gly	enzymes	1129:1135	arg1	involved-Man-Ia	enzymes			involved-Man-Ia	Fterm		enzymes			Of the 26 enzymes, the following were involved-Man-Ia, MGAT1, MGAT2, MGAT4, MGAT5, B3GalT, B4GalT, Man-II, SiaT, ST3GalI, ST3GalVI, and FucT8.
36303733	6	26	gly	enzymes	1129:1135	arg1	FucT8	enzymes			FucT8	Fterm		enzymes			Of the 26 enzymes, the following were involved-Man-Ia, MGAT1, MGAT2, MGAT4, MGAT5, B3GalT, B4GalT, Man-II, SiaT, ST3GalI, ST3GalVI, and FucT8.
28348171	11	41	gly	glycoproteins	1919:1931	arg1	surface glycoproteins	surface glycoproteins				Fterm		glycoproteins			These findings highlight the presence of macrophage infiltration in human mCRPC but also surface glycoproteins that could be used for prognosis of localized disease and for targeting strategies.
31406333	3	0	gly	glycosylation	688:700	arg2	an N-linked glycosylation site			an N-linked glycosylation site						site	This antigenic drift was mainly caused by amino acid mutations at NA residues 245, 247 (S245N/S247T; introducing an N-linked glycosylation site at residue 245) and 468.
34551980	8	61	gly	glycoprotein	1901:1912	arg1	glycoprotein structural space	glycoprotein structural space				Fterm		glycoprotein			Importantly, we anticipate that our glycomutagenesis workflow should provide access to unexplored regions of glycoprotein structural space and to custom-made neoglycoproteins with desirable properties.
34551980	8	81	gly	neoglycoproteins	1950:1965	arg1	custom-made neoglycoproteins	custom-made neoglycoproteins				Fterm		neoglycoproteins			Importantly, we anticipate that our glycomutagenesis workflow should provide access to unexplored regions of glycoprotein structural space and to custom-made neoglycoproteins with desirable properties.
31596566	5	12	gly	heterogeneity	871:883	arg1	the secreted IgG	IgG			heterogeneity	Cterm		IgG			Through iterative engineering and model refinement, we rationally increase the fraction of bigalactosylated glycans five-fold from 11.9% to 61.9% and simultaneously decrease the glycan heterogeneity on the secreted IgG.
30889014	0	115	gly	glycosylation	52:64	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	Critical amino acid residues and potential N-linked glycosylation sites contribute to circulating recombinant form 01_AE pathogenesis in Northeast China.
33577335	2	58	gly	glycoforms	369:378	arg1	the protein glycoforms	the protein glycoforms				Fterm		protein			However, deciphering the functions of N-glycosylation remains a challenge due to the lack of analytical and biochemical methods to accurately differentiate the protein glycoforms with various intact glycans.
34857845	5	3	gly	derived	1008:1014	arg1	18 serum proteins AND their nonfucosylated glycoforms	18 serum proteins			their nonfucosylated glycoforms	Fterm		proteins			We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
34857845	5	60	gly	proteins	763:770	arg1	the core fucosylation	proteins			the core fucosylation	Fterm		proteins			We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
34857845	5	62	gly	nonfucosylated	982:995	arg1	their nonfucosylated glycoforms				their nonfucosylated glycoforms						We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
34857845	5	66	gly	fucosylation	747:758	arg1	proteins	proteins				Fterm		proteins			We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
34857845	5	12	gly	glycopeptides	959:971	arg1	the core fucosylated glycoforms			glycopeptides	the core fucosylated glycoforms					glycopeptides	We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
34857845	5	45	gly	fucosylated	930:940	arg1	45 glycopeptides			glycopeptides	the core fucosylated glycoforms					glycopeptides	We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
34857845	5	65	gly	glycoforms	942:951	arg1	the core fucosylated glycoforms			glycopeptides	the core fucosylated glycoforms					glycopeptides	We performed experiments that facilitate quantitative analysis of the core fucosylation of proteins with partial structural resolution of the glycans and we present results of the mass spectrometric SWATH-type DIA analysis of relative abundances of the core fucosylated glycoforms of 45 glycopeptides to their nonfucosylated glycoforms derived from 18 serum proteins in liver disease of different etiologies.
29753740	6	16	gly	N-glycoproteins	966:980	arg1	total N-glycoproteins	total N-glycoproteins				Fterm		N-glycoproteins			Unexpectedly, we found a reduction of total N-glycoproteins and phosphorylation of GFAT1 by AMP-activated protein kinase (AMPK).
30127386	0	28	gly	eIF4GI	25:30	arg1	O-GlcNAc modification	eIF4GI			O-GlcNAc modification	PUBTATOR		eIF4GI	1981		O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response.
28679762	5	24	gly	glycoprotein	1003:1014	arg1	this glycoprotein	this glycoprotein				Fterm		glycoprotein			By establishing a purification procedure, performing further protein separation by two-dimensional electrophoresis, and utilizing mass spectrometry, we now show that this glycoprotein is identical to AAVR.
33560857	1	12	gly	N-glycosylation	164:178	arg2	28 potential N-glycosylation sites			28 potential N-glycosylation sites						sites	With 28 potential N-glycosylation sites, human carcinoembryonic antigen (CEA) bears an extreme amount of N-linked glycosylation, and approximately 60% of its molecular mass can be attributed to its carbohydrates.
29875238	5	18	gly	glycoprotein	712:723	arg1	LASV glycoprotein	LASV glycoprotein				Fterm		glycoprotein			Here, we demonstrate that another PtdSer receptor, TIM-1, mediates LASV glycoprotein (GP)-pseudotyped virion entry into αDG-knocked-out HEK 293T and wild-type (WT) Vero cells, which express αDG lacking appropriate glycosylation.
30069741	2	34	gly	carry	279:283	arg1	human therapeutic proteins AND plant-like rather than human-like glycans	human therapeutic proteins			plant-like rather than human-like glycans	Fterm		proteins			Therefore, human therapeutic proteins produced in plants often carry plant-like rather than human-like glycans, which can affect protein stability, biological function, and immunogenicity.
33073996	4	40	gly	carries	547:553	arg1	PD-L1 AND mostly complex glycans	PD-L1			mostly complex glycans	PUBTATOR		PD-L1	29126		We demonstrate that PD-L1 on the surface of breast cancer cell line carries mostly complex glycans with a high proportion of polyLacNAc structures at the N219 sequon.
31604106	2	144	gly	O-glycosylation	334:348	arg2	four O-glycosylation sites			four O-glycosylation sites						sites	The β subunit contains four O-glycosylation sites.
30552185	1	58	gly	glycoproteins	259:271	arg1	host-derived glycoproteins	host-derived glycoproteins				Fterm		glycoproteins			The asaccharolytic anaerobe Porphyromonas gingivalis metabolizes proteins it encounters in the periodontal pocket, including host-derived glycoproteins such as mucins and immunoglobulins.
30796923	7	23	gly	HDAC6	836:840	arg1	O-GlcNAcylation	HDAC6			O-GlcNAcylation	PUBTATOR		HDAC6	10013		In vitro enzymatic assays showed that O-GlcNAcylation of either tubulin or HDAC6 promoted microtubule disassembly, which likely in turn caused ciliary shortening.
33347638	3	117	gly	PSA	492:494	arg1	the disadvantage	PSA			the disadvantage	OGER		PSA	P07288		The aim of this study was to develop a better resolution for diagnosing prostate cancer to overcome the disadvantage of PSA.
31164895	0	27	gly	Fucosylation	86:97	arg1	Glycoproteins	Glycoproteins				Fterm		Glycoproteins			Characterization of a GDP-Fucose Transporter and a Fucosyltransferase Involved in the Fucosylation of Glycoproteins in the Diatom Phaeodactylum tricornutum.
31164895	0	75	gly	Glycoproteins	102:114	arg1	the Fucosylation	Glycoproteins			the Fucosylation	Fterm		Glycoproteins			Characterization of a GDP-Fucose Transporter and a Fucosyltransferase Involved in the Fucosylation of Glycoproteins in the Diatom Phaeodactylum tricornutum.
31186110	6	38	gly	α2b	1132:1134	arg1	human type N-glycans	IFN α2b			human type N-glycans	PUBTATOR		IFN α2b	3440		Heterogeneity of glycosylation and hypermannosylation in the wild-type strains of Pichia pastoris was circumvented by employing glycoengineered strain (SuperMan5) to produce glycosylated IFN α2b with human type N-glycans.
31186110	6	86	gly	Heterogeneity	941:953	arg1	hypermannosylation				hypermannosylation						Heterogeneity of glycosylation and hypermannosylation in the wild-type strains of Pichia pastoris was circumvented by employing glycoengineered strain (SuperMan5) to produce glycosylated IFN α2b with human type N-glycans.
31186110	6	99	gly	glycosylated	1115:1126	arg1	glycosylated IFN α2b	glycosylated IFN α2b				PUBTATOR		IFN α2b	3440		Heterogeneity of glycosylation and hypermannosylation in the wild-type strains of Pichia pastoris was circumvented by employing glycoengineered strain (SuperMan5) to produce glycosylated IFN α2b with human type N-glycans.
32719555	5	36	gly	glycoprotein	1023:1034	arg1	the orthogonal glycoprotein biosynthesis pathways	the orthogonal glycoprotein biosynthesis pathways				Fterm		glycoprotein			We anticipate that the orthogonal glycoprotein biosynthesis pathways developed here will provide facile access to structurally diverse O-glycoforms for a range of important scientific and therapeutic applications.
30309696	1	5	gly	phosphoglycoproteins	197:216	arg1	phosphoglycoproteins	phosphoglycoproteins				Fterm		phosphoglycoproteins			Dentin sialophosphoprotein (DSPP) is a member of the SIBLING (Small integrin-binding ligand N-linked glycoproteins) family of phosphoglycoproteins and has been proved to contribute to the migration of a variety of solid tumor cells.
30309696	1	15	gly	glycoproteins	172:184	arg1	Small integrin-binding ligand N-linked glycoproteins	Small integrin-binding ligand N-linked glycoproteins				Fterm		glycoproteins			Dentin sialophosphoprotein (DSPP) is a member of the SIBLING (Small integrin-binding ligand N-linked glycoproteins) family of phosphoglycoproteins and has been proved to contribute to the migration of a variety of solid tumor cells.
34532545	5	10	gly	proteins	1012:1019	arg1	the O-GlcNAcylation level	proteins			the O-GlcNAcylation level	Fterm		proteins			This WGA-layer thereby separates O-GlcNAcylated and non-modified forms of proteins, allowing the detection and quantification of the O-GlcNAcylation level of these proteins.
34532545	5	38	gly	proteins	922:929	arg1	O-GlcNAcylated and non-modified forms	proteins			O-GlcNAcylated and non-modified forms	Fterm		proteins			This WGA-layer thereby separates O-GlcNAcylated and non-modified forms of proteins, allowing the detection and quantification of the O-GlcNAcylation level of these proteins.
34210959	1	49	gly	O-glycosylated	116:129	arg1	heavily O-glycosylated proteins	heavily O-glycosylated proteins				Fterm		proteins	2149		Mucins are a large family of heavily O-glycosylated proteins that cover all mucosal surfaces and constitute the major macromolecules in most body fluids.
31196789	3	25	gly	glycoproteins	466:478	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			To elucidate this hypothesis, in this study, firstly we demonstrated that rLcc9 and nLcc9 were glycoproteins, and then enzymatically deglycosylated them.
28187132	3	25	gly	O-glycosylation	683:697	arg1	IgA1	IgA1				PUBTATOR		IgA1	P01876		These genes encode molecular partners essential for enzymatic O-glycosylation of IgA1.
30552185	11	72	gly	glycoproteins	2773:2785	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We hypothesized that given the dramatic shift in community structure during disease, it is possible that free carbohydrates, which would typically be readily metabolized by Gram-positive cocci after cleavage from glycoproteins, may increase in concentration locally and thereby affect the physiological state of the subgingival microbiota.
29339807	2	46	gly	glycoproteins	367:379	arg1	core-GlcNAc	glycoproteins			core-GlcNAc	Fterm		glycoproteins			Recent studies have shown that aberrant expression of α (1,6) fucosyltransferase (FUT8), which catalyzes the transfer of fucose from GDP-fucose to core-GlcNAc of the N-linked glycoproteins, modulates cellular behavior that could lead to the development of aggressive prostate cancer.
31176190	2	5	gly	glycoproteins	431:443	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			However, such compounds only resemble part of the more complex substrate molecule for enzymes acting on glycoconjugates of glycopeptides or glycoproteins.
31176190	2	15	gly	glycopeptides	414:426	arg2	glycopeptides			glycopeptides						glycopeptides	However, such compounds only resemble part of the more complex substrate molecule for enzymes acting on glycoconjugates of glycopeptides or glycoproteins.
28614667	4	22	gly	glycoforms	693:702	arg1	purified calcitonin and amylin receptor extracellular domain	ECD		domain		OGER		ECD	O95905	domain	Here, we define the role of CTR N-glycosylation in hormone binding using purified calcitonin and amylin receptor extracellular domain (ECD) glycoforms and fluorescence polarization/anisotropy and isothermal titration calorimetry peptide-binding assays.
31600726	0	85	gly	glycoforms	16:25	arg1	Recombinant FSH glycoforms	Recombinant FSH glycoforms				PUBTATOR		FSH	14308		Recombinant FSH glycoforms are bioactive in mouse preantral ovarian follicles.
34341581	4	42	gly	glycosites	742:751	arg2	glycosites			glycosites						glycosites	By dividing an N-glycan into three modules and identifying each module using distinct patterns of Y ions or a combination of distinguishable B/Y ions, the method enables determination of detailed glycan structures on thousands of glycosites in mouse brain, which comprise four types of core structure and 17 branch structures with three glycan subtypes.
32553552	0	26	gly	glycoproteins	88:100	arg1	Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins	Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins				Fterm		glycoproteins			N-glycosylation in Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins.
32553552	0	77	gly	N-glycosylation	0:14	arg1	Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins	Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins				Fterm		glycoproteins			N-glycosylation in Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins.
31164895	5	7	gly	fucosylation	864:875	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			In this paper, we have studied molecular actors potentially involved in the fucosylation of the glycoproteins in P. tricornutum.
31164895	5	54	gly	glycoproteins	884:896	arg1	the fucosylation	glycoproteins			the fucosylation	Fterm		glycoproteins			In this paper, we have studied molecular actors potentially involved in the fucosylation of the glycoproteins in P. tricornutum.
29304374	7	26	gly	fucosylated	1236:1246	arg1	total core fucosylated N-glycans				total core fucosylated N-glycans						Functional studies using primary fibroblasts from two affected individuals revealed a complete lack of FUT8 protein expression that ultimately resulted in substantial deficiencies in total core fucosylated N-glycans.
31302509	7	0	gly	glycosylation	990:1002	arg2	an N-linked glycosylation site			an N-linked glycosylation site						site	While this substitution introduces an N-linked glycosylation site, XPR1 receptors are not glycosylated indicating that this replacement alters the virus-receptor interface independently of glycosylation.
31302509	7	25	gly	glycosylated	1033:1044	arg1	XPR1 receptors	XPR1 receptors				PUBTATOR		XPR1 receptors	Q9UBH6		While this substitution introduces an N-linked glycosylation site, XPR1 receptors are not glycosylated indicating that this replacement alters the virus-receptor interface independently of glycosylation.
32030495	1	51	gly	N-glycopeptide	329:342	arg2	the intact N-glycopeptide level			the intact N-glycopeptide level						N-glycopeptide	Isotopic-labeling quantitative N-glycoproteomics characterization of cell-surface differentially expressed N-glycosylation in MCF-7/ADR cancer stem cells (CSCs) relative to MCF-7/ADR cells was carried out at the intact N-glycopeptide level with trypsin digestion, ZIC-HILIC enrichment, isotopic diethyl labeling, RPLC-MS/MS analysis of the 1:1 mixture, and GPSeeker DB search.
34212152	4	35	gly	glycoprotein	872:883	arg1	the glycoprotein PDI	the glycoprotein PDI				Fterm		structure of the glycoprotein			In addition, altering the tertiary structure of the glycoprotein PDI affected its N-glycan remodeling in a site-specific way.
32298669	1	12	gly	glycoproteins	163:175	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			Glycosylation of therapeutic glycoproteins significantly affects their physico-chemical properties, bioactivity and immunogenicity.
32298669	1	65	gly	Glycosylation	134:146	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			Glycosylation of therapeutic glycoproteins significantly affects their physico-chemical properties, bioactivity and immunogenicity.
29936833	7	10	gly	sites	1117:1121	arg1	individual proteins	proteins			sites	Fterm		proteins			Here, we describe advanced methods for identifying O-GlcNAc sites on individual proteins and across the proteome and for determining their stoichiometry in vivo.
31862392	12	29	gly	PAO1	1921:1924	arg1	extracellular polysaccharides	PAO1			extracellular polysaccharides	OGER		PAO1	Q9NWM0		The MELE diminished the production of virulence factors, including pyocyanin, protease, elastase, rhamnolipids, and extracellular polysaccharides of P. aeruginosa PAO1 in a concentration-dependent manner.
29718541	3	12	gly	glycoproteins	665:677	arg1	the Nox5-related oxidase Nox2	the Nox5-related oxidase Nox2				PUBTATOR		Nox2	1536		In contrast, Sar1 (H79G) effectively inhibits ER-to-Golgi transport of glycoproteins including the Nox5-related oxidase Nox2.
29718541	3	12	gly	glycoproteins	665:677	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In contrast, Sar1 (H79G) effectively inhibits ER-to-Golgi transport of glycoproteins including the Nox5-related oxidase Nox2.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	N1158			N1158						N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	the N-glycosite			the N-glycosite						N-glycosite	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	1,158 position			1,158 position						position	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	122, 282 and 1,158 positions			122, 282 and 1,158 positions						positions	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	120	gly	positions	1557:1565	arg1	122, 282 and 1,158 positions			122, 282 and 1,158 positions						positions	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	N1158	2		N122, N282 and N1158				2	170589	N122, N282 and N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	N122	2		N122, N282 and N1158				2	170589	N122, N282 and N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	110	gly	N-glycosite	1493:1503	arg2	N122	2		N122, N282 and N1158				2	170589	N122, N282 and N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	120	gly	positions	1557:1565	arg1	N282	2		N122, N282 and N1158				2	170589	N122, N282 and N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	120	gly	positions	1557:1565	arg1	N122	2		N122, N282 and N1158				2	170589	N122, N282 and N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
34631661	8	120	gly	positions	1557:1565	arg1	N122	2		N122, N282 and N1158				2	170589	N122, N282 and N1158	It was found that the N-glycosite at 1,158 position (N1158) and at 122, 282 and 1,158 positions (N122, N282 and N1158) were absent on S from CHO and HEK cells, respectively.
32441515	6	56	gly	glycopeptides	1014:1026	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Using CE modulation, we were able to break selectively specific glycan linkages on intact glycopeptides and get, to some degree, structure-specific mass spectrometric signals.
32441515	6	83	gly	linkages	995:1002	arg1	intact glycopeptides			intact glycopeptides	intact glycopeptides		Site			glycopeptides	Using CE modulation, we were able to break selectively specific glycan linkages on intact glycopeptides and get, to some degree, structure-specific mass spectrometric signals.
30884134	5	73	gly	glycosylated	1073:1084	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			The results revealed that N-glycosylation inhibition not only directly affects the expression of glycosylated proteins but also alters an extended scale of proteins.
34192331	9	8	gly	IgGs	1480:1483	arg1	tri- and tetra-antennary N-glycans	IgGs			tri- and tetra-antennary N-glycans	Cterm		IgGs			Concerning the Fc-mediated effector functions, the majority of IgGs with tri- and tetra-antennary N-glycans on their Fc region showed properties similar to IgGs with ordinary bi-antennary N-glycans.
34192331	9	44	gly	IgGs	1573:1576	arg1	ordinary bi-antennary N-glycans	IgGs			ordinary bi-antennary N-glycans	Cterm		IgGs			Concerning the Fc-mediated effector functions, the majority of IgGs with tri- and tetra-antennary N-glycans on their Fc region showed properties similar to IgGs with ordinary bi-antennary N-glycans.
34192331	9	59	gly	N-glycans	1515:1523	arg1	their Fc region			their Fc region	their Fc region		Site			region	Concerning the Fc-mediated effector functions, the majority of IgGs with tri- and tetra-antennary N-glycans on their Fc region showed properties similar to IgGs with ordinary bi-antennary N-glycans.
30201803	1	57	gly	glycoproteins	294:306	arg1	glycoproteins	glycoproteins			N-linked oligosaccharide side chains	Fterm		glycoproteins			BACKGROUND The glycoproteinoses are a subgroup of lysosomal storage diseases (LSDs) resulting from impaired degradation of N-linked oligosaccharide side chains of glycoproteins, which are commonly screened by detecting the accumulated free oligosaccharides (FOSs) in urine via thin layer chromatography (TLC).
31879931	2	22	gly	glycopeptides	282:294	arg2	Structurally well-defined homogeneous glycopeptides			Structurally well-defined homogeneous glycopeptides						glycopeptides	Structurally well-defined homogeneous glycopeptides are highly demanded for functional studies and biomedical applications.
34768939	5	29	gly	glycosylation	620:632	arg1	the ACE2 receptor	the ACE2 receptor				PUBTATOR		ACE2 receptor	59272		We further report that glycosylation of the ACE2 receptor enhances SARS-CoV-2 infectivity.
34551980	2	84	gly	glycoproteins	474:486	arg1	glycoproteins				glycoproteins						However, the routine use of glycosylation as a strategy for engineering proteins with advantageous properties is limited by our inability to construct and screen large collections of glycoproteins for cataloguing the consequences of glycan installation.
32946227	1	46	gly	glycopeptides	213:225	arg2	self-assembling glycopeptides			self-assembling glycopeptides						glycopeptides	Carbohydrates are often utilized to provide hydrophilicity and hydroxyl-based hydrogen bonds in self-assembling glycopeptides, affording versatile scaffolds with wide applicability in biomedical research.
31029427	0	29	gly	s	43:43	arg1	FOXA1	FOXA1			s	PUBTATOR		FOXA1	3169		Identification of the O-GalNAcylation site(s) on FOXA1 catalyzed by ppGalNAc-T2 enzyme in vitro.
30384195	5	67	gly	glycosylation	938:950	arg2	two N-linked glycosylation motifs			two N-linked glycosylation motifs						motifs	Sequence alignments with human serine proteases revealed that CT448 has a putative N-terminal pro-peptide which is extended compared to the mammalian homologs and within this pro-peptide two N-linked glycosylation motifs were found.
33222001	10	34	gly	core-fucosylated	1703:1718	arg1	a complex core-fucosylated N-glycan (Hex)2(HexNAc)2(Deoxyhexose)1+(Man)3				a complex core-fucosylated N-glycan (Hex)2(HexNAc)2(Deoxyhexose)1+(Man)3						A comparison of N-linked glycans between primary tumours with and without LNM indicated a reduced abundance of a complex core-fucosylated N-glycan (Hex)2(HexNAc)2(Deoxyhexose)1+(Man)3(GlcNAc)2, in primary tumour with associated lymph node metastasis.
33606939	5	30	gly	glycosylation	826:838	arg1	protein dynamics	protein dynamics				Fterm		protein			The results show that glycosylation nonuniformly shields the surface of the complex and only marginally affects protein dynamics.
30059200	5	32	gly	sites	880:884	arg1	the O-GlcNAcylation stoichiometry levels			sites	the O-GlcNAcylation stoichiometry levels					sites	In addition, we applied isoTCL to compare the O-GlcNAcylation stoichiometry levels of more than 100 modification sites between placenta samples from male and female mice and confirmed site-specifically that female placenta has a higher O-GlcNAcylation than its male counterpart.
31662433	5	29	gly	has	659:661	arg1	ATIII AND N-glycans	ATIII			N-glycans	PUBTATOR		ATIII	462		Although ATIII has N-glycans and a hydrophobic core, we found that its quality control depended solely on free thiol content.
34229070	11	8	gly	structures	1774:1783	arg1	APP	APP			structures	OGER		APP	P05067		GENERAL SIGNIFICANCE The accurate O-glycosites and O-glycan structures on APP may lead to a better understanding of the roles O-glycosylation plays in the processing and functions of APP.
32890705	10	21	gly	glycoproteins	1796:1808	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			GENERAL SIGNIFICANCE Our data provide new insights into how GnT-V recognizes glycoproteins.
30069741	5	32	gly	glycans	1000:1006	arg1	proteins	proteins			glycans	Fterm		proteins			Although the research community has focused on the removal of plant glycans and their replacement with human counterparts, the presence of plant glycans on proteins can also provide benefits, such as boosting the immunogenicity of some vaccines, facilitating the interaction between therapeutic proteins and their receptors, and increasing the efficacy of antibody effector functions.
29681862	5	62	gly	modification	1090:1101	arg3	proteins AND increased O-GlcNAc modification	proteins			increased O-GlcNAc modification	Fterm		proteins			We hypothesized that chronic consumption of glucose, a condition that progresses to metabolic syndrome, leads to increased O-GlcNAc modification of proteins in the PVAT, decreasing its anti-contractile effects.
29681862	5	116	gly	proteins	1106:1113	arg1	increased O-GlcNAc modification	proteins			increased O-GlcNAc modification	Fterm		proteins			We hypothesized that chronic consumption of glucose, a condition that progresses to metabolic syndrome, leads to increased O-GlcNAc modification of proteins in the PVAT, decreasing its anti-contractile effects.
34869209	1	9	gly	N-glycosylation	209:223	arg2	22 predicted N-glycosylation sites			22 predicted N-glycosylation sites						sites	The SARS-CoV-2 spike protein is heavily glycosylated, having 22 predicted N-glycosylation sites per monomer.
34869209	1	57	gly	glycosylated	175:186	arg1	The SARS-CoV-2 spike protein	The SARS-CoV-2 spike protein				PUBTATOR		spike protein	43740568		The SARS-CoV-2 spike protein is heavily glycosylated, having 22 predicted N-glycosylation sites per monomer.
34379416	9	76	gly	glycosylation	1363:1375	arg1	yPDI	yPDI				Cterm		yPDI	64714		We find that glycosylation of yPDI facilitates its catalytic site to reach close to this surface recess.
31826991	4	2	gly	region	527:532	arg1	head region high-mannose glycosites				head region high-mannose glycosites						Since the presence of head region high-mannose glycosites dictates SP-D activity, the ability to predict these glycosite glycan subtypes may be of value.
31826991	4	78	gly	head	522:525	arg1	head region high-mannose glycosites				head region high-mannose glycosites						Since the presence of head region high-mannose glycosites dictates SP-D activity, the ability to predict these glycosite glycan subtypes may be of value.
30275702	1	15	gly	glycoprotein	258:269	arg1	the small integrin binding ligand N-linked glycoprotein family	the small integrin binding ligand N-linked glycoprotein family				Fterm		glycoprotein			BACKGROUND Osteopontin (OPN), a member of the small integrin binding ligand N-linked glycoprotein family, has been analyzed in numerous types of human malignancy.
28344780	0	43	gly	glycosylation	9:21	arg1	Joubert syndrome type 10	Joubert syndrome type 10				PUBTATOR		Joubert syndrome type 10	8481		Abnormal glycosylation in Joubert syndrome type 10.
29784879	8	27	gly	glycoproteins	1535:1547	arg1	misfolded glycoproteins	misfolded glycoproteins				Fterm		glycoproteins			On the basis of these findings, we propose that disposal of misfolded glycoproteins through mannose trimming is tightly connected to redox-mediated regulation in the ER.
28883554	3	61	gly	glycosite	520:528	arg2	one potential N-linked glycosite			one potential N-linked glycosite						glycosite	The mouse-adapted pH1N1 lost its eight consecutive amino acids including one potential N-linked glycosite from the NA stalk region.
32960038	7	6	gly	fucosylated	1836:1846	arg1	afucosylated and fucosylated sulfoglycans				afucosylated and fucosylated sulfoglycans						To promote the PGC-LC-MS/MS-based method for glycome-wide applications, we extended analyses to native sulfoglycans from the egg-propagated and cell culture-derived influenza vaccines and demonstrate the high-resolution separation and structural characterization of underivatized neutral and anionic glycoforms including oligomannosidic glycan anomers, sialoglycan linkage isomers, and regioisomers of afucosylated and fucosylated sulfoglycans containing sulfated-6-GlcNAc and sulfated-4-GalNAc residues.
32960038	7	10	gly	afucosylated	1819:1830	arg1	afucosylated and fucosylated sulfoglycans				afucosylated and fucosylated sulfoglycans						To promote the PGC-LC-MS/MS-based method for glycome-wide applications, we extended analyses to native sulfoglycans from the egg-propagated and cell culture-derived influenza vaccines and demonstrate the high-resolution separation and structural characterization of underivatized neutral and anionic glycoforms including oligomannosidic glycan anomers, sialoglycan linkage isomers, and regioisomers of afucosylated and fucosylated sulfoglycans containing sulfated-6-GlcNAc and sulfated-4-GalNAc residues.
31164895	3	19	gly	glycoproteins	613:625	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			Indeed, such epitope has previously been found on N-glycans of endogenous glycoproteins in P. tricornutum.
30996101	8	104	gly	glycosylation	1609:1621	arg2	fewer N-linked glycosylation sites			fewer N-linked glycosylation sites						sites	No distinct amino acid signatures in the men's viruses were found to link to the women's founders, nor did the women's env sequences have shorter variable loops or fewer N-linked glycosylation sites.
31875397	6	16	gly	N-linked	1103:1110	arg1	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	Overall, 4 N-linked and 14 O-linked glycosylated neuropeptides have been identified for the first time in the crustacean nervous system.
31875397	6	48	gly	glycosylated	1128:1139	arg1	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	Overall, 4 N-linked and 14 O-linked glycosylated neuropeptides have been identified for the first time in the crustacean nervous system.
32269229	2	70	gly	N-glycosites	409:420	arg2	N-glycosites			N-glycosites						N-glycosites	However, investigating a representative dataset of 500 human N-glycoproteins, we observed the sites of N-linked glycosylations or N-glycosites, to be predominantly present in the regions of predicted order.
32269229	2	84	gly	N-glycoproteins	340:354	arg1	500 human N-glycoproteins	500 human N-glycoproteins				Fterm		N-glycoproteins			However, investigating a representative dataset of 500 human N-glycoproteins, we observed the sites of N-linked glycosylations or N-glycosites, to be predominantly present in the regions of predicted order.
31710461	6	16	gly	glycosylation	1052:1064	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	ChymotrypsiN is a valuable addition to the toolkit of proteases to aid the mapping of N-linked glycosylation sites within proteins and proteomes.
31640124	2	3	gly	glycosylation	261:273	arg1	its envelope (E) protein	its envelope (E) protein				Fterm		protein			ZIKV uses glycosylation of its envelope (E) protein to interact with host cell receptors to facilitate entry; these interactions could also be important for designing therapeutics and vaccines.
31133022	7	92	gly	glycans	1408:1414	arg1	GPI-anchored proteins	proteins			glycans	Fterm		proteins			Notably, by coupling biochemical and computational studies, we propose a hypothetical mechanism that involves dual selective recognition and efficient binding dependent on both N-linked glycans on GPI-anchored proteins (GPI-APs) and sphingomyelin (SM) in lipid rafts.
31133022	7	92	gly	glycans	1408:1414	arg1	GPI-APs	APs			glycans	OGER		APs	P07288		Notably, by coupling biochemical and computational studies, we propose a hypothetical mechanism that involves dual selective recognition and efficient binding dependent on both N-linked glycans on GPI-anchored proteins (GPI-APs) and sphingomyelin (SM) in lipid rafts.
31133022	7	92	gly	glycans	1408:1414	arg1	sphingomyelin	sphingomyelin			glycans	Fterm		sphingomyelin			Notably, by coupling biochemical and computational studies, we propose a hypothetical mechanism that involves dual selective recognition and efficient binding dependent on both N-linked glycans on GPI-anchored proteins (GPI-APs) and sphingomyelin (SM) in lipid rafts.
27690717	4	70	gly	Glycosylation	724:736	arg1	these sites			these sites						sites	Glycosylation of these sites strongly and differentially regulate gating kinetics, outward rectification, toxin sensitivity and physical association between the α and β2 subunits.
34110173	3	50	gly	de-N-glycosylated	670:686	arg1	de-N-glycosylated erythropoietin	de-N-glycosylated erythropoietin				PUBTATOR		de-N-glycosylated erythropoietin	2056		Permethylated N-glycans, peptides, and enriched glycopeptides of erythropoietin were analyzed by nanoLC-MS/MS, and de-N-glycosylated erythropoietin was measured by LC-MS, enabling the qualitative and quantitative analysis of glycosylation and different glycan modifications (e.g., phosphorylation and O-acetylation).
34110173	3	79	gly	erythropoietin	620:633	arg1	Permethylated N-glycans	erythropoietin			Permethylated N-glycans	PUBTATOR		erythropoietin	2056		Permethylated N-glycans, peptides, and enriched glycopeptides of erythropoietin were analyzed by nanoLC-MS/MS, and de-N-glycosylated erythropoietin was measured by LC-MS, enabling the qualitative and quantitative analysis of glycosylation and different glycan modifications (e.g., phosphorylation and O-acetylation).
34110173	3	7	gly	glycopeptides	603:615	arg2	enriched glycopeptides	erythropoietin		glycopeptides		PUBTATOR		erythropoietin	2056	glycopeptides	Permethylated N-glycans, peptides, and enriched glycopeptides of erythropoietin were analyzed by nanoLC-MS/MS, and de-N-glycosylated erythropoietin was measured by LC-MS, enabling the qualitative and quantitative analysis of glycosylation and different glycan modifications (e.g., phosphorylation and O-acetylation).
30657688	7	62	gly	proteins	1438:1445	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins			Our method provides a promising tool for the site-specific characterization of O-GlcNAcylation of important proteins.
32692906	2	46	gly	glycoproteins	556:568	arg1	their surface glycoproteins	their surface glycoproteins				Fterm		glycoproteins			Human carcinoma cells often express truncated O-glycans, Tn (GalNAcα1-Ser/Thr), and Sialyl-Tn (Siaα2-6GalNAcα1-Ser/Thr, STn) on their surface glycoproteins, yet molecular mechanisms in terms of advantages for tumor cells to have these truncated O-glycans remain elusive.
30504766	3	5	gly	proteins	373:380	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins	108155		O-linked beta-D-N-acetylglucosamine (O-GlcNAc) transferase (OGT) couples nutrient cues to O-GlcNAcylation of intracellular proteins at serine/threonine residues.
30504766	3	11	gly	residues	402:409	arg1	O-GlcNAcylation			residues	O-GlcNAcylation					residues	O-linked beta-D-N-acetylglucosamine (O-GlcNAc) transferase (OGT) couples nutrient cues to O-GlcNAcylation of intracellular proteins at serine/threonine residues.
31285765	11	37	gly	glycosylation	1429:1441	arg2	Asn29	PCOLCE		Asn29				PCOLCE	5118	Asn29	The overexpression of wild-type PCOLCE, but not its N29Q mutant, promoted migration, invasion and metastasis, indicating that the glycosylation of PCOLCE at Asn29 is necessary for its functions in osteosarcoma.
31285765	11	37	gly	glycosylation	1429:1441	arg1	PCOLCE	PCOLCE		Asn29		PUBTATOR		PCOLCE	5118	Asn29	The overexpression of wild-type PCOLCE, but not its N29Q mutant, promoted migration, invasion and metastasis, indicating that the glycosylation of PCOLCE at Asn29 is necessary for its functions in osteosarcoma.
31285765	11	37	gly	glycosylation	1429:1441	arg1	PCOLCE	PCOLCE		Asn29		PUBTATOR		PCOLCE	5118	Asn29	The overexpression of wild-type PCOLCE, but not its N29Q mutant, promoted migration, invasion and metastasis, indicating that the glycosylation of PCOLCE at Asn29 is necessary for its functions in osteosarcoma.
30042931	7	84	gly	glycosylation	1311:1323	arg1	JEV E proteins	JEV E proteins				Fterm		proteins			Overall, the patterns of N-linked glycosylation on JEV E proteins may affect viral interaction with cellular lectins and contribute to viral replication and pathogenesis.
31010828	6	35	gly	proteins	1499:1506	arg1	increased O-GlcNAcylation	proteins			increased O-GlcNAcylation	Fterm		proteins			Our results suggest that increased O-GlcNAcylation of cellular proteins may be a potential link between excess nutrient levels and pathological inflammation.
32269229	5	4	gly	N-glycosites	1191:1202	arg2	any N-glycosites			any N-glycosites						N-glycosites, N-glycosite	When compared with control disordered residue stretches without any N-glycosites, N-glycosite neighborhoods were depleted of charged, polar, hydrophobic and flexible residues and enriched for aromatic, accessible and order-promoting residues with a tendency to be part of coiled and β structures.
32269229	5	5	gly	N-glycosite	1205:1215	arg2	N-glycosite			N-glycosite						N-glycosites, N-glycosite	When compared with control disordered residue stretches without any N-glycosites, N-glycosite neighborhoods were depleted of charged, polar, hydrophobic and flexible residues and enriched for aromatic, accessible and order-promoting residues with a tendency to be part of coiled and β structures.
29718541	6	0	gly	N-glycosylated	1081:1094	arg1	one high mannose				one high mannose						Both participate in Nox1 trafficking, as Nox1 advances to the cell surface in two differentially N-glycosylated forms, one complex and one high mannose, in a Sar1/Stx5-dependent and -independent manner, respectively.
33629527	6	54	gly	NA	1081:1082	arg1	N-glycan patterns	NA			N-glycan patterns	Cterm		NA	4758		As expected, N-glycan patterns of HA and NA from virus particles produced in both MDCK cell lines were similar.
32544330	6	8	gly	N-glycosylation	855:869	arg1	Asn76			Asn76				Ribonuclease 1	P07998	Asn76	The N-glycosylation of Asn76 has less pronounced consequences.
32553552	6	12	gly	N-glycosylation	982:996	arg2	the potential N-glycosylation sites			the potential N-glycosylation sites						sites	We also performed endoglycosidase release of N-glycans in the presence of H218O determining the compositions of released N-glycans by MALDI-TOF MS analysis and established the occupancy of the potential N-glycosylation sites.
32553552	6	29	gly	occupancy	955:963	arg2	the potential N-glycosylation sites			the potential N-glycosylation sites						sites	We also performed endoglycosidase release of N-glycans in the presence of H218O determining the compositions of released N-glycans by MALDI-TOF MS analysis and established the occupancy of the potential N-glycosylation sites.
31516400	0	71	gly	glycoprotein	81:92	arg1	mass spectrometry-based human N-linked glycoprotein	mass spectrometry-based human N-linked glycoprotein				Fterm		glycoprotein			N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping.
31516400	0	73	gly	glycosylation	98:110	arg2	glycosylation site mapping			glycosylation site mapping						site	N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping.
34687015	4	27	gly	Asn297-linked	614:626	arg1	the IgG Asn297-linked glycan			Asn297	the IgG Asn297-linked glycan			Immunoglobulin G Glycosylation		Asn297	In many inflammatory and autoimmune conditions, as well as in metabolic, cardiovascular, infectious, and neoplastic diseases, the IgG Asn297-linked glycan becomes less sialylated and less galactosylated, leading to increased expression of glycans terminating with GlcNAc.
34780171	7	29	gly	glycoproteins	1605:1617	arg1	1118 glycoproteins	1118 glycoproteins				Fterm		glycoproteins			To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
34780171	7	53	gly	glycopeptides	1544:1556	arg1	2434 N-glycosites			2434 N-glycosites						N-glycosites	To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
34780171	7	53	gly	glycopeptides	1544:1556	arg2	7367 unique glycopeptides			7367 unique glycopeptides						glycopeptides	To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
34780171	7	70	gly	sialoglycopeptides	1507:1524	arg2	2742 sialoglycopeptides			2742 sialoglycopeptides						sialoglycopeptides	To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
34780171	7	81	gly	N-glycosites	1584:1595	arg2	2434 N-glycosites			N-glycosites	166 glycans					N-glycosites	To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
34780171	7	81	gly	N-glycosites	1584:1595	arg1	1118 glycoproteins	glycoproteins		N-glycosites	166 glycans	Fterm		glycoproteins		N-glycosites	To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
34780171	7	81	gly	N-glycosites	1584:1595	arg1	1118 glycoproteins	glycoproteins		N-glycosites		Fterm		glycoproteins		N-glycosites	To the best of our knowledge, the result with 2742 sialoglycopeptides among 7367 unique glycopeptides and 166 glycans from 2434 N-glycosites of 1118 glycoproteins (Byonic score > 100) provides one of the deepest glycoproteomic profiles in single-cell type.
33305950	1	68	gly	N-glycosylation	99:113	arg1	Asn-297			Asn-297						Asn-297	The N-glycosylation pattern of Asn-297 may have impacts on monoclonal antibody (mAb) drug plasma clearance, antibody-dependent cell mediated cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC).
32384234	6	16	gly	N-glycopeptides	1555:1569	arg2	all related N-glycopeptides			all related N-glycopeptides						N-glycopeptides	Moreover, the characteristic peptide core ion carrying a ring cleavage remnant of the innermost amino sugar residue of an N-glycan can be relied upon to filter out all related N-glycopeptides carrying additional O-glycans defined by specific mass increments.
32384234	6	56	gly	carrying	1571:1578	arg1	all related N-glycopeptides AND additional O-glycans			all related N-glycopeptides	additional O-glycans					N-glycopeptides	Moreover, the characteristic peptide core ion carrying a ring cleavage remnant of the innermost amino sugar residue of an N-glycan can be relied upon to filter out all related N-glycopeptides carrying additional O-glycans defined by specific mass increments.
30535277	4	34	gly	fucosylated	753:763	arg1	fucosylated glycans				fucosylated glycans						The results showed that the expression level of fucosylated glycans recognized by LTL was significantly increased in HCC compared with other subjects (P < 0.0001).
34780171	2	1	gly	glycopeptides	374:386	arg2	enrich glycopeptides			enrich glycopeptides						glycopeptides	Due to the structural macro- and microheterogeneity, low stoichiometry, and low ionization efficiency of glycopeptides, high-performance tools to enrich glycopeptides, especially the negatively charged and labile sialoglycopeptides, are essential to enhance the identification of the underexplored glycoproteome.
34780171	2	14	gly	glycopeptides	326:338	arg2	glycopeptides			glycopeptides						glycopeptides	Due to the structural macro- and microheterogeneity, low stoichiometry, and low ionization efficiency of glycopeptides, high-performance tools to enrich glycopeptides, especially the negatively charged and labile sialoglycopeptides, are essential to enhance the identification of the underexplored glycoproteome.
34780171	2	25	gly	microheterogeneity	254:271	arg1	glycopeptides			glycopeptides						glycopeptides	Due to the structural macro- and microheterogeneity, low stoichiometry, and low ionization efficiency of glycopeptides, high-performance tools to enrich glycopeptides, especially the negatively charged and labile sialoglycopeptides, are essential to enhance the identification of the underexplored glycoproteome.
34780171	2	91	gly	sialoglycopeptides	434:451	arg2	the negatively charged and labile sialoglycopeptides			the negatively charged and labile sialoglycopeptides						sialoglycopeptides	Due to the structural macro- and microheterogeneity, low stoichiometry, and low ionization efficiency of glycopeptides, high-performance tools to enrich glycopeptides, especially the negatively charged and labile sialoglycopeptides, are essential to enhance the identification of the underexplored glycoproteome.
32776107	5	5	gly	position	749:756	arg1	di-N-acetyl glucuronic acid			position	di-N-acetyl glucuronic acid					position	glycolytica is responsible for the addition of di-N-acetyl glucuronic acid at the third position of a tetrasaccharide.
33581334	4	20	gly	O-glycoprotein	513:526	arg1	O-glycoprotein data	O-glycoprotein data				Fterm		O-glycoprotein			It was constructed with a collection of O-glycoprotein data from different sources.
30770376	4	61	gly	glycoproteins	801:813	arg1	plasma glycoproteins	plasma glycoproteins				Fterm		glycoproteins			METHODS After PNGase F digestion of plasma glycoproteins, N-glycans were linked to a quinolone using a transient amine group at the reducing end, isolated by a hydrophilic interaction chromatography column, and then identified by accurate mass and quantified using a stable isotope-labeled glycopeptide as the internal standard.
30770376	4	76	gly	glycopeptide	1048:1059	arg2	a stable isotope-labeled glycopeptide			a stable isotope-labeled glycopeptide						glycopeptide	METHODS After PNGase F digestion of plasma glycoproteins, N-glycans were linked to a quinolone using a transient amine group at the reducing end, isolated by a hydrophilic interaction chromatography column, and then identified by accurate mass and quantified using a stable isotope-labeled glycopeptide as the internal standard.
34687015	9	17	gly	glycosylated	1218:1229	arg1	The aberrantly glycosylated IgG glycans				The aberrantly glycosylated IgG glycans						The aberrantly glycosylated IgG glycans associated with inflammation and aging can sustain inflammation through different mechanisms, fueling a vicious loop.
32281997	11	96	gly	glycoproteins	1815:1827	arg1	purified glycoproteins	purified glycoproteins				Fterm		glycoproteins			The method is applicable to purified glycoproteins as well as to biofluids and cell- or tissue-based samples.
29753740	4	60	gly	glycoprotein	791:802	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Up-regulation of glutamine: fructose 6-phosphate aminotransferase 1 (GFAT1), which belongs to the hexosamine biosynthesis pathway (HBP) that produces uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) to maintain glycoprotein, was validated by evaluation of mRNA and protein levels.
29339411	4	24	gly	glycopeptide	1007:1018	arg2	22 unique glycopeptide species			22 unique glycopeptide species						glycopeptide	A comprehensive glycomic and glycoproteomic assessment revealed extensive glycan macro- and microheterogeneity identified from 22 unique glycopeptide species.
32172531	4	1	gly	glycosylated	616:627	arg1	normal glycosylated hERG channels	normal glycosylated hERG channels				PUBTATOR		hERG channels	2078		In the present study, our data revealed that, compared with normal glycosylated hERG channels, nonglycosylated hERG channels were significantly more susceptible to cleavage by extracellular PK.
32172531	4	52	gly	nonglycosylated	644:658	arg1	nonglycosylated hERG channels	nonglycosylated hERG channels				PUBTATOR		hERG channels	2078		In the present study, our data revealed that, compared with normal glycosylated hERG channels, nonglycosylated hERG channels were significantly more susceptible to cleavage by extracellular PK.
30224358	2	47	gly	factor	459:464	arg1	a single known substrate-the so-called HCF-1PRO repeat	host-cell factor 1			a single known substrate-the so-called HCF-1PRO repeat	PUBTATOR		host-cell factor 1	3054		OGT thereby stably glycosylates serines and threonines of numerous proteins and, via a transient glutamate glycosylation, cleaves a single known substrate-the so-called HCF-1PRO repeat of the transcriptional co-regulator host-cell factor 1 (HCF-1).
30224358	2	75	gly	glycosylates	247:258	arg1	serines			serines and threonines						serines and threonines	OGT thereby stably glycosylates serines and threonines of numerous proteins and, via a transient glutamate glycosylation, cleaves a single known substrate-the so-called HCF-1PRO repeat of the transcriptional co-regulator host-cell factor 1 (HCF-1).
35026558	1	7	gly	serine	145:150	arg1	residue			residue						threonine residue	Mucin-type O-glycosylation of serine or threonine residue in proteins is known to be one of the major post-translational modifications.
35026558	1	33	gly	O-glycosylation	126:140	arg1	proteins	proteins		serine		Fterm		proteins		serine	Mucin-type O-glycosylation of serine or threonine residue in proteins is known to be one of the major post-translational modifications.
35026558	1	33	gly	O-glycosylation	126:140	arg1	proteins	proteins		threonine residue		Fterm		proteins		threonine residue	Mucin-type O-glycosylation of serine or threonine residue in proteins is known to be one of the major post-translational modifications.
31029427	6	69	gly	found	987:991	arg1	FOXA1 AND two O-GalNAcylation sites	FOXA1			two O-GalNAcylation sites	PUBTATOR		FOXA1	3169		By dividing and expressing recombinant FOXA1 as three segments, two O-GalNAcylation sites were found on FOXA1, both located at the C-terminal of the protein.
28469279	3	14	gly	glycosylated	398:409	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			As lectins bind glycosylated proteins, we asked if specific lectins interact with mitotic O-GlcNAcylated proteins during metaphase to ensure correct cell division.
31918287	9	75	gly	sialoglycoproteins	1251:1268	arg1	O-linked sialoglycoproteins	O-linked sialoglycoproteins				Fterm		sialoglycoproteins			RESULTS Siglec-E ligands are O-linked sialoglycoproteins with molecular weights of 70-300 kDa and are distributed broadly on mouse aorta as well as on MAECs in vitro.
33197804	1	32	gly	glycoproteins	110:122	arg1	large glycosylated glycoproteins	large glycosylated glycoproteins				Fterm		glycoproteins			OBJECTIVE Mucins are large glycosylated glycoproteins that are produced in the salivary glands, and their changes may contribute to the development of xerostomia due to aging and the accompanying deterioration of oral hygiene.
33197804	1	36	gly	glycosylated	97:108	arg1	large glycosylated glycoproteins	large glycosylated glycoproteins				Fterm		glycoproteins			OBJECTIVE Mucins are large glycosylated glycoproteins that are produced in the salivary glands, and their changes may contribute to the development of xerostomia due to aging and the accompanying deterioration of oral hygiene.
30127001	2	27	gly	domain	344:349	arg1	a novel O-linked hexose modification			domain	a novel O-linked hexose modification					domain	A recent structural analysis suggested that a novel O-linked hexose modification on serine 435 of the mammalian NOTCH1 core ligand-binding domain lies at the interface with its ligands.
30127001	2	32	gly	modification	273:284	arg1	serine 435			serine 435	serine 435		SpecificSite	POGLUT1	56983	serine 435	A recent structural analysis suggested that a novel O-linked hexose modification on serine 435 of the mammalian NOTCH1 core ligand-binding domain lies at the interface with its ligands.
30127001	2	74	gly	modification	273:284	arg1	the mammalian NOTCH1 core ligand-binding domain AND a novel O-linked hexose modification			the mammalian NOTCH1 core ligand-binding domain	a novel O-linked hexose modification					domain	A recent structural analysis suggested that a novel O-linked hexose modification on serine 435 of the mammalian NOTCH1 core ligand-binding domain lies at the interface with its ligands.
29491160	5	80	gly	α2-6-sialylated	747:761	arg1	α2-6-sialylated branched N-linked glycans				α2-6-sialylated branched N-linked glycans						We have recently shown that human influenza viruses preferentially bind to α2-6-sialylated branched N-linked glycans, where the sialic acids on each branch can bind to receptor sites on two protomers of the same hemagglutinin (HA) trimer.
32827291	3	3	gly	presence	571:578	arg1	both sites AND N-glycans			both sites	N-glycans					sites	We found that mutation of one site (Asn220) had greater impact on enzymatic activity while another (Asn335) on Golgi localization and presence of N-glycans at both sites is required for production of stable and enzymatically active protein and its secretion.
30659065	5	29	gly	occupancy	1225:1233	arg2	site occupancy			site occupancy						site	Here we employ a nLC-MS/MS method using stepped-energy higher energy collisional dissociation to characterize the N-glycan repertoire and site occupancy of circulating serum antibodies.
31892091	7	8	gly	glycoprotein	1511:1522	arg1	bAGP	bAGP				Cterm		bAGP	497200		Tryptic digests of other glycoproteins (i.e. human apolipoprotein CIII (APO-C3) and bovine alpha-1-acid glycoprotein (bAGP)) were also analyzed, demonstrating the applicability to glycopeptides with different glycan composition and nature.
31892091	7	8	gly	glycoprotein	1511:1522	arg1	bovine alpha-1-acid glycoprotein	bovine alpha-1-acid glycoprotein				PUBTATOR		alpha-1-acid glycoprotein	497200		Tryptic digests of other glycoproteins (i.e. human apolipoprotein CIII (APO-C3) and bovine alpha-1-acid glycoprotein (bAGP)) were also analyzed, demonstrating the applicability to glycopeptides with different glycan composition and nature.
31892091	7	22	gly	glycoproteins	1432:1444	arg1	other glycoproteins	other glycoproteins				Fterm		glycoproteins			Tryptic digests of other glycoproteins (i.e. human apolipoprotein CIII (APO-C3) and bovine alpha-1-acid glycoprotein (bAGP)) were also analyzed, demonstrating the applicability to glycopeptides with different glycan composition and nature.
31892091	7	43	gly	glycopeptides	1587:1599	arg1	different glycan composition			glycopeptides	different glycan composition					glycopeptides	Tryptic digests of other glycoproteins (i.e. human apolipoprotein CIII (APO-C3) and bovine alpha-1-acid glycoprotein (bAGP)) were also analyzed, demonstrating the applicability to glycopeptides with different glycan composition and nature.
34962767	1	3	gly	glycoproteins	210:222	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Characterization of mucin-type O-glycans linked to serine/threonine of glycoproteins is technically challenging, in part, due to a lack of effective enzymatic tools that enable their analysis.
30659065	10	52	gly	N-glycosylation	1818:1832	arg1	N92			N92						N92	In digests of the sera from a subset of donors, we detected an unmodified peptide containing a proline residue at position 93; this substitution would strongly disfavor N-glycosylation at N92.
31852790	8	83	gly	glycosylation	1437:1449	arg2	glycosylation sites			glycosylation sites						sites and residues in	Primarily, the pattern of glycosylation sites and residues in the Sa antigenic region, around the receptor binding site (RBS), served as signatures for the elicitation of broadly reactive antibodies by these HA immunogens.
31018136	2	8	gly	enzymes	398:404	arg1	key determinants	enzymes			key determinants	Fterm		enzymes			This complex carbohydrate mixture-the glycan profile-is generated in the compartmentalized Golgi, in which level and localization of glycosylation enzymes are key determinants.
28364041	5	60	gly	glycoproteins	990:1002	arg1	CD144/VE-cadherin	CD144/VE-cadherin				PUBTATOR		CD144	1003		Using two different galectin-3 affinity purification processes, we extracted four cell membrane glycoproteins, CD146/melanoma cell adhesion molecule (MCAM)/MUC18, CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1), CD144/VE-cadherin, and CD106/Endoglin, from vascular endothelial cells.
28364041	5	60	gly	glycoproteins	990:1002	arg1	CD106/Endoglin	CD106/Endoglin				PUBTATOR		CD106	7412		Using two different galectin-3 affinity purification processes, we extracted four cell membrane glycoproteins, CD146/melanoma cell adhesion molecule (MCAM)/MUC18, CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1), CD144/VE-cadherin, and CD106/Endoglin, from vascular endothelial cells.
28364041	5	60	gly	glycoproteins	990:1002	arg1	CD146/melanoma cell adhesion molecule (MCAM)/MUC18	CD146/melanoma cell adhesion molecule (MCAM)/MUC18				PUBTATOR		CD146/melanoma cell adhesion molecule	4162		Using two different galectin-3 affinity purification processes, we extracted four cell membrane glycoproteins, CD146/melanoma cell adhesion molecule (MCAM)/MUC18, CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1), CD144/VE-cadherin, and CD106/Endoglin, from vascular endothelial cells.
28364041	5	60	gly	glycoproteins	990:1002	arg1	four cell membrane glycoproteins	four cell membrane glycoproteins				Fterm		glycoproteins			Using two different galectin-3 affinity purification processes, we extracted four cell membrane glycoproteins, CD146/melanoma cell adhesion molecule (MCAM)/MUC18, CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1), CD144/VE-cadherin, and CD106/Endoglin, from vascular endothelial cells.
28364041	5	60	gly	glycoproteins	990:1002	arg1	CD31/platelet endothelial cell adhesion molecule-1	CD31/platelet endothelial cell adhesion molecule-1				PUBTATOR		CD31/platelet endothelial cell adhesion molecule-1	5175		Using two different galectin-3 affinity purification processes, we extracted four cell membrane glycoproteins, CD146/melanoma cell adhesion molecule (MCAM)/MUC18, CD31/platelet endothelial cell adhesion molecule-1 (PECAM-1), CD144/VE-cadherin, and CD106/Endoglin, from vascular endothelial cells.
31726007	6	39	gly	glycosylation	958:970	arg2	seven glycosylation sites			seven glycosylation sites						sites	As determined by mass spectrometric analysis, seven glycosylation sites were identified in type I collagen and the most predominant site was at the helical cross-linking site, α1-87.
31399531	0	37	gly	glycosylation	57:69	arg2	a new N-linked glycosylation site			a new N-linked glycosylation site						site	Ile73Asn mutation in protein C introduces a new N-linked glycosylation site on the first EGF-domain of protein C and causes thrombosis.
30487280	9	106	gly	envelope-glycoprotein	1774:1794	arg1	envelope-glycoprotein spikes	envelope-glycoprotein spikes				PUBTATOR		envelope-glycoprotein	100616444		In neither study, however, was redirection associated with increased neutralization of heterologous tier 2 viruses.IMPORTANCE Engineered SOSIP trimers mimic envelope-glycoprotein spikes, which stud the surface of HIV-1 particles and mediate viral entry into cells.
33908016	3	12	gly	fucosylated	891:901	arg1	α1,6-linked core fucosylated glycans				α1,6-linked core fucosylated glycans						Sialic acid chemical derivatization can be used to determine the isomeric linkage (α2,3 or α2,6) of sialic acids attached to N-glycans, while endoglycosidase F3 (Endo F3) can be enzymatically applied to preferentially release α1,6-linked core fucosylated glycans, further describing the linkage of fucose on N-glycans.
34192331	4	38	gly	region	657:662	arg1	bi-antennary complex-type N-glycans			region	bi-antennary complex-type N-glycans					region	The major glycoforms of the N-glycans on the IgG-Fc region are bi-antennary complex-type N-glycans, while multibranched complex-type N-glycans are not typically found.
34192331	4	61	gly	glycoforms	615:624	arg1	the N-glycans				the N-glycans						The major glycoforms of the N-glycans on the IgG-Fc region are bi-antennary complex-type N-glycans, while multibranched complex-type N-glycans are not typically found.
34192331	4	74	gly	N-glycans	633:641	arg1	the IgG-Fc region			the IgG-Fc region	the IgG-Fc region		Site			region	The major glycoforms of the N-glycans on the IgG-Fc region are bi-antennary complex-type N-glycans, while multibranched complex-type N-glycans are not typically found.
31837192	3	44	gly	glycoproteins	620:632	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			However, with exception of monoclonal antibodies, homogenous human-like β1,4-galactosylation is very hard to achieve in recombinant glycoproteins.
34232571	6	59	gly	glycosites	936:945	arg2	glycosites			glycosites						glycosites	Together, our workflow enables a high-throughput enrichment of glycans, glycosites, and intact glycopeptides from complex biological or clinical samples.
34232571	6	64	gly	glycopeptides	959:971	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Together, our workflow enables a high-throughput enrichment of glycans, glycosites, and intact glycopeptides from complex biological or clinical samples.
34645615	8	85	gly	2,6-sialylation	1543:1557	arg1	triantennary glycans				triantennary glycans						The strongest association for prevalent cardiovascular disease was a high level of bisection on a group of diantennary glycans (A2FS0B; OR=1.38, p=1.34×10-11), while for prevalent nephropathy the increase in 2,6-sialylation on triantennary glycans was most pronounced (A3E; OR=1.28, p=9.70×10-6).
29577901	4	21	gly	peptide	688:694	arg1	a novel O-GlcNAc modified peptide 20LWKPGAQDASSQAQGGSSCILRE42				a novel O-GlcNAc modified peptide 20LWKPGAQDASSQAQGGSSCILRE42						In this study, we report that human LXRα is O-GlcNAc modified in its N-terminal domain (NTD) by identifying a specific O-GlcNAc site S49 and a novel O-GlcNAc modified peptide 20LWKPGAQDASSQAQGGSSCILRE42.
30471266	10	62	gly	glycoproteins	1482:1494	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			CCs encircle the ACCs and contain microvilli, small endocytic vesicles and notably a large numbers of adhesive granules that, according to element analysis and histochemistry, contain glycoproteins.
30221662	8	69	gly	modification	1873:1884	arg1	SNAP29 AND O‑GlcNAc modification	SNAP29			O‑GlcNAc modification	PUBTATOR		SNAP29	O95721		Consistent with the myocardium of diabetic rats, it was also shown in the NRCMs that O‑GlcNAc modification of SNAP29 negatively regulated autophagic flux.
30221662	8	89	gly	SNAP29	1889:1894	arg1	O‑GlcNAc modification	SNAP29			O‑GlcNAc modification	PUBTATOR		SNAP29	O95721		Consistent with the myocardium of diabetic rats, it was also shown in the NRCMs that O‑GlcNAc modification of SNAP29 negatively regulated autophagic flux.
34341430	1	51	gly	fucosylation	161:172	arg1	selected fucosyltransferases				selected fucosyltransferases						In the seminal plasma (n = 118) and serum (n = 90) clusterin (CLU) the fucosylation and the expression of selected fucosyltransferases (FUTs) were analyzed.
34813786	3	27	gly	asialoglycoproteins	525:543	arg1	asialoglycoproteins	asialoglycoproteins			O-glycans	Fterm		asialoglycoproteins			Enzymatic characterization for substrate specificity and kinetic property indicate that cST3Gal-I prefers O-glycans, rather than N-glycan, of asialoglycoproteins as substrates.
30036038	10	11	gly	glycoproteins	1428:1440	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Specific extraction of glycopeptides and glycoproteins containing certain glycans from complex samples was demonstrated.
30036038	10	49	gly	glycopeptides	1410:1422	arg2	glycopeptides			glycopeptides						glycopeptides	Specific extraction of glycopeptides and glycoproteins containing certain glycans from complex samples was demonstrated.
30036038	10	38	gly	containing	1442:1451	arg1	glycopeptides AND certain glycans			glycopeptides	certain glycans					glycopeptides	Specific extraction of glycopeptides and glycoproteins containing certain glycans from complex samples was demonstrated.
30224358	7	13	gly	serine-acceptor	1267:1281	arg1	proposed OGT-based or UDP-GlcNAc-based serine-acceptor residue activation mechanisms			serine	proposed OGT-based or UDP-GlcNAc-based serine-acceptor residue activation mechanisms					serine	Using serine- or glutamate-containing HCF-1PRO-repeat sequences, we show that proposed OGT-based or UDP-GlcNAc-based serine-acceptor residue activation mechanisms can be circumvented independently, but not when disrupted together.
30224358	7	65	gly	serine-	1156:1162	arg1	serine- or glutamate-containing HCF-1PRO-repeat sequences			serine	serine- or glutamate-containing HCF-1PRO-repeat sequences					serine	Using serine- or glutamate-containing HCF-1PRO-repeat sequences, we show that proposed OGT-based or UDP-GlcNAc-based serine-acceptor residue activation mechanisms can be circumvented independently, but not when disrupted together.
33340519	6	30	gly	hypersialylated	932:946	arg1	hypersialylated or oligomannose-type glycans				hypersialylated or oligomannose-type glycans						We then probed the impact of ACE2 glycosylation on S binding and revealed a subtle sensitivity with hypersialylated or oligomannose-type glycans slightly impeding the interaction.
29779369	2	15	gly	glycoproteins	540:552	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In this work, we further extend the application of the DiPyrO tag, which provides amine group reactivity, optical detection capability, and improved electrospray sensitivity, to quantify N-linked glycans enzymatically released from glycoproteins in the glycosylamine form.
29779369	2	62	gly	released	526:533	arg1	glycoproteins AND N-linked glycans	glycoproteins			N-linked glycans	Fterm		glycoproteins			In this work, we further extend the application of the DiPyrO tag, which provides amine group reactivity, optical detection capability, and improved electrospray sensitivity, to quantify N-linked glycans enzymatically released from glycoproteins in the glycosylamine form.
34927585	2	18	gly	Glycosylation	263:275	arg1	both proteins	both proteins				Fterm		proteins			Glycosylation of both proteins is critical for this interaction.
26040437	3	13	gly	unglycosylated	533:546	arg1	A 40-kDa unglycosylated precursor	A 40-kDa unglycosylated precursor				Fterm		precursor			A 40-kDa unglycosylated precursor and a fully processed, mature form of the HA1 protein migrated around 52 kDa were detected by SDS-PAGE and confirmed by Western blot using H5N1-specific antibody.
31516400	8	35	gly	CONCLUSIONS	1438:1448	arg1	//nglycositeatlas.biomarkercenter.org			//nglycositeatlas.biomarkercenter.org						//nglycositeatlas.biomarkercenter.org	CONCLUSIONS The entire human N-glycoproteome database as well as 22 sub-databases associated with individual tissues or body fluids can be downloaded from the N-GlycositeAtlas website at http://nglycositeatlas.biomarkercenter.org.
32269229	6	9	gly	N-glycosite	1420:1430	arg2	N-glycosite			N-glycosite						N-glycosite	N-glycosite neighborhoods also showed greater phylogenetic conservation among amniotes, compared with control ordered regions, which in turn were more conserved than disordered control regions.
34341430	6	88	gly	glycoproteins	1232:1244	arg1	glycoproteins glycosylation	glycoproteins glycosylation				Fterm		glycoproteins			Lack of similarities between levels of examined parameters in blood serum and seminal plasma may suggest the differences in mechanisms leading to glycoproteins glycosylation.
29263309	1	11	gly	glycosylated	154:165	arg1	an N-linked glycosylated protein	an N-linked glycosylated protein				Fterm		protein	728		C5a receptor 1 (C5aR1) is a G protein-coupled receptor for C5a and also an N-linked glycosylated protein.
34670086	1	21	gly	glycosylation	117:129	arg1	the envelope spike (S) protein	the envelope spike (S) protein				Fterm		protein			Understanding the glycosylation of the envelope spike (S) protein of SARS-CoV-2 is important in defining the antigenic surface of this key viral target.
32220931	2	46	gly	glycoproteins	316:328	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This simple modification can dramatically affect the activities and half-lives of glycoproteins, effects that are relevant to understanding the invasiveness of some cancers, development of mAb therapeutics, and the etiology of a congenital glycosylation disorder.
30134155	3	9	gly	threonines	378:387	arg1	O-linked β-N-acetylglucosamine			serines and threonines	O-linked β-N-acetylglucosamine					serines and threonines	O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serines and threonines that functions as a sensor of cellular nutrients.
30134155	3	29	gly	serines	366:372	arg1	O-linked β-N-acetylglucosamine			serines and threonines	O-linked β-N-acetylglucosamine					serines and threonines	O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serines and threonines that functions as a sensor of cellular nutrients.
32462590	4	19	gly	N-glycopeptides	723:737	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Current proteomic workflows are not optimized for the enrichment, identification and characterization of N-glycopeptides.
34631661	7	0	gly	N-glycosites	1390:1401	arg2	the 22 predicted N-glycosites	S proteins		N-glycosites		PUBTATOR		S proteins	43740568	N-glycosites	We identified 21 and 19 out of the 22 predicted N-glycosites of the SARS-CoV-2 S proteins produced in CHO and HEK, respectively.
30793403	2	35	gly	addition	286:293	arg1	proteins-is	proteins-is a			addition	Fterm		proteins-is a			O-GlcNAcylation-the addition of a single sugar residue (O-linked β-N-acetylglucosamine) on proteins-is a posttranslational modification that acts as a cellular nutrient sensor and likely modulates the function of oocyte proteins.
30158774	1	59	gly	glycoproteins	152:164	arg1	high-molecular-weight glycoproteins	glycoproteins			a high O-linked carbohydrate content	Fterm		glycoproteins			BACKGROUND Mucins are high-molecular-weight glycoproteins with a high O-linked carbohydrate content, which are synthesized by many secretory epithelial cells as membrane-bound and/or secreted products.
33073996	8	3	gly	carries	1282:1288	arg1	PD-L1 AND polyLacNAc glycans	PD-L1		the N219 sequon	polyLacNAc glycans	PUBTATOR	SpecificSite	PD-L1	29126	N219 sequon	In conclusion, PD-L1 expressed in the MDA-MB-231 breast cancer cell line carries polyLacNAc glycans mostly at the N219 sequon, which displays the highest variability in occupancy and is most likely to influence the interaction with PD-1.
31336133	6	23	gly	O-glycosylation	948:962	arg2	a single O-glycosylation site			a single O-glycosylation site						site	The aim of the present study was to compare the influence of ANITVNITV peptide fusion on the physicochemical, biological and pharmacokinetic properties of native hIFN-α2b (IFNwt), which contains a single O-glycosylation site, and a hyperglycosylated variant (IFN4N), that bears, in addition, 4 N-linked glycans.
31336133	6	29	gly	hyperglycosylated	976:992	arg1	a hyperglycosylated variant	a hyperglycosylated variant				Fterm		variant			The aim of the present study was to compare the influence of ANITVNITV peptide fusion on the physicochemical, biological and pharmacokinetic properties of native hIFN-α2b (IFNwt), which contains a single O-glycosylation site, and a hyperglycosylated variant (IFN4N), that bears, in addition, 4 N-linked glycans.
32544330	2	2	gly	glycosylation	283:295	arg1	Ribonuclease 1	Ribonuclease 1		asparagine residues 34, 76, and 88		PUBTATOR		Ribonuclease 1	P07998	asparagine residues 34, 76, and 88	Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88.
32544330	2	2	gly	glycosylation	283:295	arg1	RNase 1	RNase 1		asparagine residues 34, 76, and 88		PUBTATOR		RNase 1	6035	asparagine residues 34, 76, and 88	Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88.
31913636	4	52	gly	glycoprotein	939:950	arg1	the envelope glycoprotein	the envelope glycoprotein				PUBTATOR		envelope glycoprotein	100616444		Iterative rounds of de-N-glycosylation followed by N-glycanation could be repeated at least three times and were observed for other viral glycoproteins/vaccine antigens, including the envelope glycoprotein (Env) from HIV.
31913636	4	52	gly	glycoprotein	939:950	arg1	Env	Env				PUBTATOR		Env	100616444		Iterative rounds of de-N-glycosylation followed by N-glycanation could be repeated at least three times and were observed for other viral glycoproteins/vaccine antigens, including the envelope glycoprotein (Env) from HIV.
31129958	5	41	gly	peptide	911:917	arg1	complete, biologically relevant glycan and peptide sequences				complete, biologically relevant glycan and peptide sequences						DecoyDeveloper has a simple user interface and is capable of producing large sets of decoys containing complete, biologically relevant glycan and peptide sequences.
31561469	10	43	gly	glycoprotein	1146:1157	arg1	glycoprotein VI (GPVI)	glycoprotein VI (GPVI)				Fterm		glycoprotein			Platelets interact with collagen via receptors for collagen, glycoprotein VI (GPVI), and α2β1 integrin.
34110173	2	15	gly	glycosylation	295:307	arg1	first-generation erythropoietin	first-generation erythropoietin				PUBTATOR		erythropoietin	2056		This study presents an in-depth analytical strategy for glycosylation of first-generation erythropoietin (epoetin beta), including a developed mass spectrometric workflow for N-glycan analysis, bottom-up mass spectrometric methods for site-specific N-glycosylation, and a LC-MS approach for O-glycan identification.
30032777	0	36	gly	glycopeptides	124:136	arg2	glycopeptides			glycopeptides						glycopeptides	Facile preparation of microporous organic polymers functionalized macroporous hydrophilic resin for selective enrichment of glycopeptides.
32710848	3	45	gly	glycoproteins	720:732	arg1	glycoproteins				glycoproteins						We used CRISPR-Cas9 gene targeting to generate a library of 3D organotypic skin tissues that selectively differ in their capacity to produce glycan structures on the main types of N- and O-linked glycoproteins and glycolipids.
31913636	1	46	gly	glycoproteins	154:166	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In cells, asparagine/N-linked glycans are added to glycoproteins cotranslationally, in an attachment process that supports proper folding of the nascent polypeptide.
32039582	5	69	gly	glycoproteins	687:699	arg1	isolated cell membrane glycoproteins	isolated cell membrane glycoproteins				Fterm		glycoproteins			O-Glycans were chemically released from isolated cell membrane glycoproteins following N-glycan and lipid/glycolipid removal by PNGase F digestion and Folch extraction, respectively.
32039582	5	72	gly	released	650:657	arg1	isolated cell membrane glycoproteins AND O-Glycans	isolated cell membrane glycoproteins			O-Glycans	Fterm		glycoproteins			O-Glycans were chemically released from isolated cell membrane glycoproteins following N-glycan and lipid/glycolipid removal by PNGase F digestion and Folch extraction, respectively.
31931833	7	25	gly	modification	1414:1425	arg1	the glycosite Asn297			the glycosite Asn297	the glycosite Asn297		AminoAcid			glycosite Asn297	When the Endo-T was expressed in Golgi, the secreted IgG1-Fc region was efficiently produced with almost completely truncated N-glycans and the N-GlcNAc modification on the glycosite Asn297 was confirmed via Mass Spectrometry.
31931833	7	55	gly	glycosite	1434:1442	arg2	the glycosite Asn297			the glycosite Asn297						glycosite Asn297	When the Endo-T was expressed in Golgi, the secreted IgG1-Fc region was efficiently produced with almost completely truncated N-glycans and the N-GlcNAc modification on the glycosite Asn297 was confirmed via Mass Spectrometry.
29621585	5	35	gly	glycosylation	689:701	arg2	two putative N-linked glycosylation sites			two putative N-linked glycosylation sites						sites	The extracellular domain of cnBST-2 has two putative N-linked glycosylation sites and three potential dimerization sites.
30962950	2	20	gly	glycoproteins	422:434	arg1	recurrence-related N-linked glycoproteins	recurrence-related N-linked glycoproteins				Fterm		glycoproteins			A method for quantitating N-glycoproteome was used to screen for, and identify, recurrence-related N-linked glycoproteins from 100 serum samples taken from patients with early-stage HCC.
34687015	2	12	gly	galactose	323:331	arg1	residues			residues						residues	The asparagine 297 (Asn297) of IgG heavy chains is decorated by a diantennary glycan bearing a number of galactose and sialic acid residues on the branches ranging from 0 to 2.
31600726	4	73	gly	glycoforms	575:584	arg1	FSH glycoforms	FSH glycoforms				PUBTATOR		FSH	14308		The relative abundance of FSH glycoforms changes with advanced reproductive age, shifting from predominantly FSH21/18 in younger women to FSH24 in older women.
29491151	2	83	gly	glycoproteins	612:624	arg1	250 patient-derived gp120 envelope glycoproteins	250 patient-derived gp120 envelope glycoproteins				Fterm		glycoproteins			In an attempt to identify the transmitted founder (TF) virus and differentiate the biological properties and infectious potential of the TF virus from those of the population of the early transmitted viruses, 250 patient-derived gp120 envelope glycoproteins were cloned in pMN-K7-Luc-IRESs-NefΔgp120 to obtain chimeric viruses.
30946947	5	62	gly	glycoprotein	663:674	arg1	a 69-kDa (SDS-PAGE) monomeric glycoprotein	a 69-kDa (SDS-PAGE) monomeric glycoprotein				Fterm		glycoprotein			Purified Hyal-Ba is a 69-kDa (SDS-PAGE) monomeric glycoprotein with an N-terminal amino acid sequence sharing high identity with homologous snake venom hyaluronidases.
31826991	13	31	gly	head	2239:2242	arg1	influenza type A virus (IAV) through interaction with hemagglutinin (HA) head region high-mannose glycan(s)				influenza type A virus (IAV) through interaction with hemagglutinin (HA) head region high-mannose glycan(s)						Lung surfactant protein D (SP-D), a key factor in first-line innate immunity defense, removes influenza type A virus (IAV) through interaction with hemagglutinin (HA) head region high-mannose glycan(s).
31826991	13	53	gly	region	2244:2249	arg1	influenza type A virus (IAV) through interaction with hemagglutinin (HA) head region high-mannose glycan(s)				influenza type A virus (IAV) through interaction with hemagglutinin (HA) head region high-mannose glycan(s)						Lung surfactant protein D (SP-D), a key factor in first-line innate immunity defense, removes influenza type A virus (IAV) through interaction with hemagglutinin (HA) head region high-mannose glycan(s).
32030495	3	16	gly	N-glycopeptide	940:953	arg2	546 intact N-glycopeptide IDs			546 intact N-glycopeptide IDs						N-glycopeptide	The N-glycan moieties in 546 intact N-glycopeptide IDs were identified with more than one structure-diagnostic fragment ions where multiple linkage structures exist for each of the monosaccharide compositions.
32030495	3	35	gly	moieties	917:924	arg1	546 intact N-glycopeptide IDs	IDs			moieties	OGER		IDs	P22304		The N-glycan moieties in 546 intact N-glycopeptide IDs were identified with more than one structure-diagnostic fragment ions where multiple linkage structures exist for each of the monosaccharide compositions.
30713024	1	3	gly	found	178:182	arg2	Ser AND O-Linked N-acetylglucosamine			Ser	O-Linked N-acetylglucosamine					Ser	O-Linked N-acetylglucosamine (O-GlcNAc) is an abundant posttranslationalmonosaccaride-modification found on Ser or Thr residues of intracellular proteins in most eukaryotes.
30713024	1	3	gly	found	178:182	arg2	Thr AND O-Linked N-acetylglucosamine			Thr	O-Linked N-acetylglucosamine					Thr residues	O-Linked N-acetylglucosamine (O-GlcNAc) is an abundant posttranslationalmonosaccaride-modification found on Ser or Thr residues of intracellular proteins in most eukaryotes.
34885895	2	1	gly	glycosylation	330:342	arg2	glycosylation sites			glycosylation sites						sites	Computational prediction approaches serve as complementary methods for the characterization of glycosylation sites.
30683699	2	62	gly	sialylated	432:441	arg1	sialylated glycans				sialylated glycans						A population of Fcs bearing sialylated glycans has been identified as contributing to this functionality, and high levels of sialylation also lead to longer serum retention times advantageous for therapy.
31254563	4	56	gly	N1125	683:687	arg1	a single N-linked, biantennary, complex glycan			N1125	a single N-linked, biantennary, complex glycan					N1125	Mass spectrometric analysis show a single N-linked, biantennary, complex glycan at N1125.
32168410	7	80	gly	deglycosylated	892:905	arg1	deglycosylated FXIII-B	deglycosylated FXIII-B				PUBTATOR		FXIII-B	2165		The structure of deglycosylated FXIII-B was investigated by gel filtration.
31231989	2	22	gly	variants	292:299	arg1	a UDP-galactose transporter	variants			a UDP-galactose transporter	Fterm		variants			De novo variants in the SLC35A2 gene, which encodes a UDP-galactose transporter, are responsible for CDGs with an X-linked dominant manner.
31231989	2	84	gly	SLC35A2	308:314	arg1	a UDP-galactose transporter	SLC35A2			a UDP-galactose transporter	PUBTATOR		SLC35A2	7355		De novo variants in the SLC35A2 gene, which encodes a UDP-galactose transporter, are responsible for CDGs with an X-linked dominant manner.
33295603	5	100	gly	O-glycans	1017:1025	arg1	IgA1	IgA1			O-glycans	PUBTATOR		IgA1	P01876		Here, we describe a series of nano-liquid chromatography (LC)-mass spectrometry (MS) analyses that demonstrate the range of glycosyltransferase enzymatic activities involved in the biosynthesis of clustered O-glycans on IgA1.
34192331	2	30	gly	N-glycosylation	263:277	arg1	this site			this site						site	Previous studies have shown that N-glycosylation of this site is a critical mediator of the antibody's effector functions, such as antibody-dependent cellular cytotoxicity.
33581334	2	55	gly	O-glycoprotein	360:373	arg1	emerging O-glycoprotein data	emerging O-glycoprotein data				Fterm		O-glycoprotein			However, the lack of updated and convenient databases deters the storage of and reference to emerging O-glycoprotein data.
30905461	1	12	gly	glycoproteins	134:146	arg1	proteins	proteins				Fterm		proteins			Most of proteins in human blood circulation are glycoproteins with one or more covalently linked N- or O-linked glycans.
30905461	1	59	gly	glycoproteins	134:146	arg1	O-linked glycans	glycoproteins			O-linked glycans	Fterm		glycoproteins			Most of proteins in human blood circulation are glycoproteins with one or more covalently linked N- or O-linked glycans.
34687007	7	13	gly	glycoprotein	1300:1311	arg1	glycoprotein purification	glycoprotein purification				Fterm		glycoprotein			Lectin chromatography is a historical approach for the analysis of lectin-carbohydrate interactions and glycoprotein purification but is still being used as a valuable tool in Igs purification and glycan analysis.
27690717	2	32	gly	glycosylation	332:344	arg2	multiple N-linked glycosylation sites			multiple N-linked glycosylation sites						sites	Although multiple N-linked glycosylation sites in the extracellular loop of β subunits have been identified, very little is known about how glycosylation influences the structure and function of BK channels.
33045166	1	9	gly	glycopeptides	252:264	arg2	homogeneous human E-cadherin N-linked glycopeptides			homogeneous human E-cadherin N-linked glycopeptides						glycopeptides	We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
33045166	1	19	gly	N-glycosylation	328:342	arg2	the Asn-633 N-glycosylation site			the Asn-633 N-glycosylation site						site	We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
33045166	1	22	gly	site	344:347	arg1	a core-fucosylated tetrasaccharide			site	a core-fucosylated tetrasaccharide					site	We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
33045166	1	22	gly	site	344:347	arg1	a complex-type biantennary octasaccharide			site	a complex-type biantennary octasaccharide					site	We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
33045166	1	22	gly	site	344:347	arg1	a conserved trisaccharide			site	a conserved trisaccharide					site	We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
33045166	1	22	gly	site	344:347	arg1	representative N-glycan structures			site	representative N-glycan structures					site	We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
33045166	1	24	gly	core-fucosylated	429:444	arg1	a core-fucosylated tetrasaccharide				a core-fucosylated tetrasaccharide						We report herein an efficient chemical synthesis of homogeneous human E-cadherin N-linked glycopeptides consisting of a heptapeptide sequence adjacent to the Asn-633 N-glycosylation site with representative N-glycan structures, including a conserved trisaccharide, a core-fucosylated tetrasaccharide, and a complex-type biantennary octasaccharide.
29339411	9	28	gly	Y1-glycopeptide	1659:1673	arg2	pseudo Y1-glycopeptide fragment ions			pseudo Y1-glycopeptide fragment ions				Selenomonas		Y1-glycopeptide	This effect resulted in pseudo Y1-glycopeptide fragment ions that indicated the presence of additional glycosylation sites on a single glycopeptide.
29339411	9	54	gly	glycopeptide	1763:1774	arg2	a single glycopeptide			a single glycopeptide						glycopeptide	This effect resulted in pseudo Y1-glycopeptide fragment ions that indicated the presence of additional glycosylation sites on a single glycopeptide.
29339411	9	95	gly	glycosylation	1731:1743	arg2	additional glycosylation sites			additional glycosylation sites						sites	This effect resulted in pseudo Y1-glycopeptide fragment ions that indicated the presence of additional glycosylation sites on a single glycopeptide.
31133022	8	36	gly	structures	1631:1640	arg1	GPI-APs	APs			structures	OGER		APs	P07288		Furthermore, specific binding of the lectin module with biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures on GPI-APs triggers substantial conformational changes in the aerolysin module, which interacts with SM, ultimately resulting in the formation of a membrane-bound oligomer in lipid rafts.
31133022	8	47	gly	bisialylated	1558:1569	arg1	biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures				biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures						Furthermore, specific binding of the lectin module with biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures on GPI-APs triggers substantial conformational changes in the aerolysin module, which interacts with SM, ultimately resulting in the formation of a membrane-bound oligomer in lipid rafts.
31133022	8	72	gly	N-glycan	1586:1593	arg1	GPI-APs	APs			N-glycan	OGER		APs	P07288		Furthermore, specific binding of the lectin module with biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures on GPI-APs triggers substantial conformational changes in the aerolysin module, which interacts with SM, ultimately resulting in the formation of a membrane-bound oligomer in lipid rafts.
31133022	8	109	gly	nonfucosylated	1571:1584	arg1	biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures				biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures						Furthermore, specific binding of the lectin module with biantennary bisialylated nonfucosylated N-glycan or sialyl Lewis X-containing glycan structures on GPI-APs triggers substantial conformational changes in the aerolysin module, which interacts with SM, ultimately resulting in the formation of a membrane-bound oligomer in lipid rafts.
31281932	3	5	gly	proteins	433:440	arg1	appropriate terminal sialic acids	proteins			appropriate terminal sialic acids	Fterm		proteins			Expression systems to produce authentically fully glycosylated therapeutic proteins with appropriate terminal sialic acids are not yet perfected.
31281932	3	14	gly	glycosylated	408:419	arg1	fully glycosylated therapeutic proteins	fully glycosylated therapeutic proteins				Fterm		proteins			Expression systems to produce authentically fully glycosylated therapeutic proteins with appropriate terminal sialic acids are not yet perfected.
29267884	5	4	gly	glycosylation	946:958	arg2	known glycosylation sites			known glycosylation sites						sites	The data made available via this tool will be regularly updated to improve the coverage of known glycosylation sites and datasets, reflecting the advances currently being made in characterization of glycoproteomes.
34450331	2	65	gly	deglycosylation	289:303	arg1	N-glycans				N-glycans						The method measures the differential rate of deglycosylation of N-glycans on natively folded proteins by the common peptide:N-glycosidase F (PNGase F) enzyme which in turn informs on their spatial presentation and solvent exposure on the protein surface hence ultimately the glycoprotein conformation.
34450331	2	84	gly	glycoprotein	519:530	arg1	the glycoprotein conformation	the glycoprotein conformation				Fterm		glycoprotein			The method measures the differential rate of deglycosylation of N-glycans on natively folded proteins by the common peptide:N-glycosidase F (PNGase F) enzyme which in turn informs on their spatial presentation and solvent exposure on the protein surface hence ultimately the glycoprotein conformation.
33666655	3	42	gly	glycoproteins	691:703	arg1	red blood cell glycoproteins	red blood cell glycoproteins				Fterm		glycoproteins			Here, we compiled results obtained through lectin arrays, glycan arrays, and mass spectrometry to interrogate red blood cell glycoproteins and glycan-binding proteins found in the plasma of healthy individuals and patients with sickle cell disease and sickle cell trait.
34279906	0	63	gly	Glycans	87:93	arg1	Intact Glycoproteins	Glycoproteins			Glycans	Fterm		Glycoproteins			Native Mass Spectrometry Meets Glycomics: Resolving Structural Detail and Occupancy of Glycans on Intact Glycoproteins.
30850477	5	92	gly	N-glycosylation	553:567	arg2	N-glycosylation sites			N-glycosylation sites						sites	The aim of the current study is to establish patterns of N-glycosylation sites in Ab V regions of naive and memory B cell subsets.
34717971	1	8	gly	glycosylation	109:121	arg1	viral glycoproteins	viral glycoproteins				Fterm		glycoproteins			Extensive glycosylation of viral glycoproteins is a key feature of the antigenic surface of viruses and yet glycan processing can also be influenced by the manner of their recombinant production.
34717971	1	42	gly	glycoproteins	132:144	arg1	viral glycoproteins	viral glycoproteins				Fterm		glycoproteins			Extensive glycosylation of viral glycoproteins is a key feature of the antigenic surface of viruses and yet glycan processing can also be influenced by the manner of their recombinant production.
32022078	6	24	gly	glycopeptides	821:833	arg2	glycopeptides			glycopeptides						glycopeptides	First, glycopeptides bearing the most common glycans can be identified from EThcD and/or HCD data.
30633504	7	47	gly	N-glycosylation	1192:1206	arg1	a human secretory enzyme	a human secretory enzyme				Fterm		enzyme			These data provide new insight on the biological role of the N-glycosylation of a human secretory enzyme.
30487799	10	20	gly	glycosites	1697:1706	arg2	individual glycosites			individual glycosites						glycosites	These results indicate that grass carp serum IgM exhibits unique N-glycan features and highly processed oligosaccharides attached to individual glycosites.
30487799	10	31	gly	attached	1674:1681	arg2	individual glycosites AND highly processed oligosaccharides			individual glycosites	highly processed oligosaccharides					glycosites	These results indicate that grass carp serum IgM exhibits unique N-glycan features and highly processed oligosaccharides attached to individual glycosites.
32699088	12	60	gly	head	2246:2249	arg1	the head domain glycans				the head domain glycans						It also revealed that the head domain glycans affect N1 stability more than N2, suggesting N2 is more accommodating to glycan additions.
32699088	12	98	gly	domain	2251:2256	arg1	the head domain glycans				the head domain glycans						It also revealed that the head domain glycans affect N1 stability more than N2, suggesting N2 is more accommodating to glycan additions.
31665542	4	9	gly	glycoprotein	664:675	arg1	the S-layer protein Mpsy_1486	the S-layer protein Mpsy_1486				Cterm		Mpsy_1486			Lectin affinity enriched a glycoprotein in cells grown at 30 °C under ample substrate availability, which was identified as the S-layer protein Mpsy_1486.
31665542	4	9	gly	glycoprotein	664:675	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			Lectin affinity enriched a glycoprotein in cells grown at 30 °C under ample substrate availability, which was identified as the S-layer protein Mpsy_1486.
31661879	2	65	gly	N-glycosylation	392:406	arg1	protein biogenesis	protein biogenesis				Fterm		protein			Consistent with the important role of N-glycosylation in protein biogenesis, DHDDS mutations result in human diseases.
30032777	6	4	gly	glycopeptides	1017:1029	arg2	811 unique glycopeptides sequence			811 unique glycopeptides sequence						glycopeptides sequence	Furthermore, 879 unique N-glycosylation sites in 811 unique glycopeptides sequence mapped to 516 N-glycosylated proteins were identified in three replicate analyses of proteins extracted from mouse liver.
30032777	6	51	gly	N-glycosylation	981:995	arg2	879 unique N-glycosylation sites			879 unique N-glycosylation sites						sites	Furthermore, 879 unique N-glycosylation sites in 811 unique glycopeptides sequence mapped to 516 N-glycosylated proteins were identified in three replicate analyses of proteins extracted from mouse liver.
30032777	6	74	gly	516 N-glycosylated	1050:1067	arg1	516 N-glycosylated proteins	516 N-glycosylated proteins				Fterm		proteins			Furthermore, 879 unique N-glycosylation sites in 811 unique glycopeptides sequence mapped to 516 N-glycosylated proteins were identified in three replicate analyses of proteins extracted from mouse liver.
34128680	1	6	gly	used	289:292	arg2	short synthetic peptides			short synthetic peptides						peptides	Owing to their biocompatibility and biodegradability, short synthetic peptides that self-assemble into elongated β-sheet fibers (i.e., peptide nanofibers) are widely used to create biomaterials for diverse medical and biotechnology applications.
29793953	8	123	gly	nonglycosylated	1482:1496	arg1	nonglycosylated NTCP	nonglycosylated NTCP				PUBTATOR		NTCP	6554		In conclusion, nonglycosylated NTCP is expressed by differentiated HepaRG cells and capable of mediating cHBV infection in HepG2 cells, but it cannot explain differential susceptibility of HepaRG and HepG2/NTCP cells to cHBV versus sHBV infection and different HBsAg/HBeAg ratios following cHBV infection.
31196789	5	30	gly	sites	645:649	arg1	Three putative N-glycosylation sites			Three putative N-glycosylation sites						sites	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	39	gly	N-glycosylation	629:643	arg2	Three putative N-glycosylation sites			Three putative N-glycosylation sites						sites	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	30	gly	sites	645:649	arg1	N454			N293, N313, and N454				Lcc9		N293, N313, and N454	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	30	gly	sites	645:649	arg1	N293			N293, N313, and N454				Lcc9		N293, N313, and N454	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	30	gly	sites	645:649	arg1	N293			N293, N313, and N454				Lcc9		N293, N313, and N454	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	39	gly	N-glycosylation	629:643	arg2	N293			N293, N313, and N454				Lcc9		N293, N313, and N454	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	39	gly	N-glycosylation	629:643	arg2	N454			N293, N313, and N454				Lcc9		N293, N313, and N454	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
31196789	5	39	gly	N-glycosylation	629:643	arg2	N454			N293, N313, and N454				Lcc9		N293, N313, and N454	Three putative N-glycosylation sites (N293, N313, and N454) were then predicted in Lcc9 and substituted to evaluate their roles in its specific activity.
34110173	1	42	gly	glycoproteins	132:144	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			The characterization of therapeutic glycoproteins is challenging due to the structural heterogeneity of the therapeutic protein glycosylation.
29745666	2	33	gly	derived	430:436	arg1	glycoproteins AND N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			Efficient protein/glycoprotein extraction is a crucial step in the preparation of N-glycans derived from glycoproteins prior to LC-MS analysis.
29745666	2	49	gly	protein/glycoprotein	348:367	arg1	Efficient protein/glycoprotein extraction	Efficient protein/glycoprotein extraction				Fterm		protein/glycoprotein			Efficient protein/glycoprotein extraction is a crucial step in the preparation of N-glycans derived from glycoproteins prior to LC-MS analysis.
29745666	2	63	gly	glycoproteins	443:455	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Efficient protein/glycoprotein extraction is a crucial step in the preparation of N-glycans derived from glycoproteins prior to LC-MS analysis.
30682623	1	30	gly	glycosylation	129:141	arg1	several therapeutic proteins	several therapeutic proteins				Fterm		proteins			N-linked glycosylation affects the potency, safety, immunogenicity, and pharmacokinetic clearance of several therapeutic proteins including monoclonal antibodies.
32171833	3	37	gly	glycosylated	441:452	arg1	GSP	GSP				PUBTATOR		GSP	14683		Their effects on body weight, fasting blood glucose (FBG), glycosylated serum protein (GSP), serum insulin levels (HOMA-IR), blood lipids (including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c)) were tested.
32171833	3	37	gly	glycosylated	441:452	arg1	glycosylated serum protein	glycosylated serum protein				Fterm		protein			Their effects on body weight, fasting blood glucose (FBG), glycosylated serum protein (GSP), serum insulin levels (HOMA-IR), blood lipids (including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c)) were tested.
34192331	8	55	gly	sialylated	1379:1388	arg1	a sialylated penta-antennary structure				a sialylated penta-antennary structure						Among the IgGs produced by these methods, the largest N-glycan attached was a bisecting N-acetylglucosamine containing a sialylated penta-antennary structure.
34770808	4	6	gly	linked	679:684	arg2	hIgG AND these complex oligosaccharides	hIgG			these complex oligosaccharides	Cterm		IgG			To achieve the profile information of these complex oligosaccharides, linked by asparagine to hIgG in the blood, the glycoproteins of the samples needed to be cleaved, labelled, and purified with sufficient yield and selectivity.
34770808	4	54	gly	glycoproteins	726:738	arg1	the glycoproteins	the glycoproteins				Fterm		glycoproteins			To achieve the profile information of these complex oligosaccharides, linked by asparagine to hIgG in the blood, the glycoproteins of the samples needed to be cleaved, labelled, and purified with sufficient yield and selectivity.
34813786	1	51	gly	sialyltransferase	123:139	arg1	An invertebrate sialyltransferase, cST3Gal-I	sialyltransferase			An invertebrate sialyltransferase, cST3Gal-I	Fterm		sialyltransferase			An invertebrate sialyltransferase, cST3Gal-I, identified from the sea squirt Ciona savignyi, was functionally characterized in vitro using recombinant enzyme expressed in yeast strains.
32776107	1	14	gly	Glycosylation	135:147	arg1	multiple proteins	multiple proteins				Fterm		proteins			Glycosylation of multiple proteins via O-linkage is well documented in bacterial species of Neisseria of import to human disease.
31266803	2	78	gly	fucosylated	626:636	arg1	fucosylated glycans				fucosylated glycans						The pneumococcus can degrade a variety of complex glycans, including N- and O-linked glycans, glycosaminoglycans, and carbohydrate antigens, an ability that is tightly linked to the virulence of S. pneumoniae Although S. pneumoniae is known to use a sophisticated enzyme machinery to attack the human glycome, how it copes with fucosylated glycans, which are primarily histo-blood group antigens, is largely unknown.
28186505	0	26	gly	N-glycosylation	0:14	arg1	mouse TRAIL-R	mouse TRAIL-R				PUBTATOR		TRAIL	22035		N-glycosylation of mouse TRAIL-R and human TRAIL-R1 enhances TRAIL-induced death.
28186505	0	26	gly	N-glycosylation	0:14	arg1	human TRAIL-R1	human TRAIL-R1				PUBTATOR		TRAIL-R1	8797		N-glycosylation of mouse TRAIL-R and human TRAIL-R1 enhances TRAIL-induced death.
31357165	3	39	gly	glycosylation	414:426	arg2	potential N-linked glycosylation sites			potential N-linked glycosylation sites						sites	Gp120 sequences were obtained from 322 subjects; length, potential N-linked glycosylation sites (PNGs), net-charge (NC) and 179-181tripeptide α4β7-binding-motif of V2 were evaluated.
30042931	6	81	gly	glycosylation	1262:1274	arg2	this G1 site			this G1 site						site	Furthermore, the JEV mutants with G1 glycosylation showed enhanced DC-SIGN binding, which may then lead to reduced brain invasion and explain the reason why WT JEV is devoid of this G1 site of glycosylation.
31527085	5	65	gly	sites	643:647	arg1	sOGT	sOGT			sites	Cterm		sOGT	8473		To date, the major O-GlcNAcylation sites and their roles in sOGT remain unknown.
32544330	4	43	gly	glycan	567:572	arg1	Asn34			Asn34	Asn34		AminoAcid	Ribonuclease 1	P07998	Asn34	The glycan on Asn34 is relatively compact and rigid, donates hydrogen bonds that "cap" the carbonyl groups at the C-terminus of an α-helix, and enhances protein thermostability.
28528272	1	9	gly	glycosylated	218:229	arg1	The asparaginyl endopeptidase legumain and its inhibitor cystatin E/M	The asparaginyl endopeptidase legumain and its inhibitor cystatin E/M				OGER		asparaginyl endopeptidase legumain	Q99538		The asparaginyl endopeptidase legumain and its inhibitor cystatin E/M are endogenously glycosylated.
29325746	2	1	gly	glycoprotein	317:328	arg1	a high molecular weight (79.4 kDa) monomeric glycoprotein	a high molecular weight (79.4 kDa) monomeric glycoprotein				Fterm		glycoprotein			It is a high molecular weight (79.4 kDa) monomeric glycoprotein that contains 2.4% neutral sugars and 58.4% N-linked oligosaccharides and strongly binds to Concanavalin A.
29325746	2	3	gly	contains	335:342	arg1	a high molecular weight (79.4 kDa) monomeric glycoprotein AND 2.4% neutral sugars and 58.4% N-linked oligosaccharides	a high molecular weight (79.4 kDa) monomeric glycoprotein			2.4% neutral sugars and 58.4% N-linked oligosaccharides	Fterm		glycoprotein			It is a high molecular weight (79.4 kDa) monomeric glycoprotein that contains 2.4% neutral sugars and 58.4% N-linked oligosaccharides and strongly binds to Concanavalin A.
34192331	1	53	gly	N-glycosylation	155:169	arg2	Asn297			Asn297				HER2	2064	Asn297	Immunoglobulin G (IgG) has a conserved N-glycosylation site at Asn297 in the fragment crystallizable (Fc) region.
34192331	1	53	gly	N-glycosylation	155:169	arg2	a conserved N-glycosylation site			a conserved N-glycosylation site						site	Immunoglobulin G (IgG) has a conserved N-glycosylation site at Asn297 in the fragment crystallizable (Fc) region.
31336250	2	42	gly	aglycosylated	474:486	arg1	the aglycosylated Fc region			the aglycosylated Fc region						region	Despite abrogated Fc gamma receptor (FcγR) binding and therapeutic effector function in the absence of N-linked glycosylation at Asn297, the aglycosylated Fc region of IgG antibodies has bioprocessing advantages such as the absence of glycan heterogeneity and simple bacterial antibody production.
28931878	3	18	gly	N-glycans	446:454	arg1	IgGs	IgGs			N-glycans	Cterm		IgGs			We discover 20 sulfated and 4 acetylated N-glycans on IgGs.
33073996	2	52	gly	N-glycosylation	211:225	arg1	PD-L1	PD-L1				PUBTATOR		PD-L1	29126		N-glycosylation of PD-L1 affects its interaction with PD-1, but little is known about the distribution of glycoforms at its four NXS/T sequons.
30712568	5	7	gly	1:230	1293:1297	arg1	sixteen glycopeptides			sixteen glycopeptides						glycopeptides	Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N ≥ 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/N ≥ 20).
30712568	5	27	gly	glycopeptides	1207:1219	arg2	twenty glycopeptides			twenty glycopeptides						glycopeptides	Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N ≥ 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/N ≥ 20).
30712568	5	45	gly	digest	1192:1197	arg1	twenty glycopeptides			twenty glycopeptides						glycopeptides	Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N ≥ 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/N ≥ 20).
30712568	5	54	gly	glycopeptides	1308:1320	arg2	sixteen glycopeptides			sixteen glycopeptides						glycopeptides	Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N ≥ 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/N ≥ 20).
30712568	5	69	gly	glycopeptides	1158:1170	arg2	glycopeptides			glycopeptides						glycopeptides	Owning to the abundant of ZIC molecules with multi-charge and polar groups on the surface of resulting polymer coating, the Fe3O4@PMSA demonstrated high selectivity for glycopeptides enrichment with IgG digest (twenty glycopeptides identified, S/N ≥ 20) and a mixture of IgG and BSA at the mass ratio of 1:230 (sixteen glycopeptides identified, S/N ≥ 20).
27728760	5	36	gly	N-glycoproteins	1092:1106	arg1	the surface N-glycoproteins	the surface N-glycoproteins				Fterm		N-glycoproteins			The "don't eat me" molecule CD47 and its binding partners thrombospondin-1 (TSP1) and signal regulatory protein α (SIRPα) were among the surface N-glycoproteins detected.
34192302	11	23	gly	found	1617:1621	arg2	serum M2BP AND The LacdiNAc structure	serum M2BP			The LacdiNAc structure	PUBTATOR		M2BP	3959		The LacdiNAc structure was not found in serum M2BP.
31471298	7	5	gly	glycans	1218:1224	arg1	ACPA-IgG	ACPA			glycans	PUBTATOR		ACPA	5657		RESULTS V-domain glycans on ACPA-IgG were already present up to 15 years before disease in pre-symptomatic individuals and their abundance increased closer to symptom onset.
34324829	8	8	gly	glycoproteins	1627:1639	arg1	homogeneous N-glycan glycoproteins				homogeneous N-glycan glycoproteins						Altogether, the experimental data provide pivotal insight into the molecular mechanism of substrate recognition and specificity for GH18 ENGases and further advance our understanding of chemoenzymatic synthesis and remodeling of homogeneous N-glycan glycoproteins.
31450586	9	28	gly	glycoproteins	1213:1225	arg1	the altered glycoproteins	the altered glycoproteins				Fterm		glycoproteins			Most of the altered glycoproteins were also observed with changes in their global protein expression levels.
31671706	4	43	gly	glycosylation	761:773	arg2	The expected N-linked glycosylation sites			The expected N-linked glycosylation sites						sites	The expected N-linked glycosylation sites were successfully identified and site-specific glycopeptide mapping was completed by Integrated GlycoProteome Analyzer (I-GPA) for the resulting raw tandem mass data.
31671706	4	61	gly	glycopeptide	828:839	arg2	site-specific glycopeptide mapping			site-specific glycopeptide mapping						glycopeptide	The expected N-linked glycosylation sites were successfully identified and site-specific glycopeptide mapping was completed by Integrated GlycoProteome Analyzer (I-GPA) for the resulting raw tandem mass data.
31176190	8	23	gly	glycopeptides	1681:1693	arg2	glycopeptides			glycopeptides						glycopeptides	The devised assay and data handling method determines the accessible substrate concentration as well as the steady state kinetic parameters, KM and kcat, for glycoconjugates of glycopeptides described by the same units as obtained from using small-molecule substrates and thus allows for a direct comparison.
32269229	0	64	gly	glycoproteins	70:82	arg1	human glycoproteins	human glycoproteins				Fterm		glycoproteins			Mutually exclusive locales for N-linked glycans and disorder in human glycoproteins.
32825463	2	8	gly	fucosylated	184:194	arg1	fucosylated glycans				fucosylated glycans						Alterations in levels of fucosylated glycans are associated with various diseases.
30348809	11	61	gly	glycosylation	1423:1435	arg1	CSF3R	CSF3R				OGER		CSF3R	Q99062		SIGNIFICANCE: This study reveals the critical importance of membrane-proximal N-linked glycosylation of CSF3R for the maintenance of ligand dependency in leukemia.
32035902	2	36	gly	N-glycosylation	224:238	arg1	its extracellular domain			domain						domain	N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism.
32035902	2	36	gly	N-glycosylation	224:238	arg1	ECD	ECD		domain		OGER		ECD	O95905	domain	N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism.
32035902	2	36	gly	N-glycosylation	224:238	arg1	ECD	ECD		domain		OGER		ECD	O95905	domain	N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism.
32035902	2	36	gly	N-glycosylation	224:238	arg1	ECD	ECD		asparagine 130		OGER		ECD	O95905	asparagine 130	N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism.
32258897	9	7	gly	di-sialylated	1402:1414	arg1	a di-sialylated, core-fucosylated bi-antennary structure				a di-sialylated, core-fucosylated bi-antennary structure						The major N-glycan, accounting for ∼42% of total N-glycans, had a di-sialylated, core-fucosylated bi-antennary structure.
31667130	7	111	gly	N-glycosylation	1178:1192	arg1	β-catenin	β-catenin				PUBTATOR		-catenin	84353		It was confirmed that APPB inhibits N-glycosylation of β-catenin at 2.5 nM concentration.
31337705	0	64	gly	trisaccharide	17:29	arg1	oviduct membrane glycoproteins	glycoproteins			trisaccharide	Fterm		glycoproteins			Sulfated Lewis A trisaccharide on oviduct membrane glycoproteins binds bovine sperm and lengthens sperm lifespan.
31337705	0	65	gly	glycoproteins	51:63	arg1	oviduct membrane glycoproteins	oviduct membrane glycoproteins				Fterm		glycoproteins			Sulfated Lewis A trisaccharide on oviduct membrane glycoproteins binds bovine sperm and lengthens sperm lifespan.
32364434	6	30	gly	glycoproteins	1206:1218	arg1	purified glycoproteins	purified glycoproteins				Fterm		glycoproteins			Here, we are reporting the wide potential of micropermethylation-based high-throughput structural analysis of glycans from various sources, including human plasma, mammalian cells, and purified glycoproteins, through an automated tandem electrospray ionization-mass spectrometry (ESI-MSn) platform.
32172531	9	42	gly	glycosylation	1392:1404	arg1	hERG channel stability	hERG channel				PUBTATOR		hERG channel	2078		In summary, our results revealed that N-linked glycosylation protects hERG against protease-mediated degradation and thus contributes to hERG channel stability on the plasma membrane.
34670086	10	0	gly	glycosylation	1688:1700	arg1	the spike protein	the spike protein				PUBTATOR		spike protein	43740568		Overall, we show that in matched expression systems the quaternary protein architecture limits O-linked glycosylation of the spike protein.
33983715	6	75	gly	sialylated	1018:1027	arg1	neutral and sialylated N-glycans				neutral and sialylated N-glycans						The integrated analysis of neutral and sialylated N-glycans and fOSs showed the potential to elucidate the cause of ER stress, which cannot be readily done by protein markers alone.
30619255	12	4	gly	glycoforms	2443:2452	arg1	distinct O-glycosylation states/CD45 glycoforms	distinct O-glycosylation states/CD45 glycoforms				PUBTATOR		CD45	5788		Thus, our data suggest that O-glycan remodeling is a feature of B cell differentiation, dually regulated by ST3Gal1 and GCNT1, that ultimately results in expression of distinct O-glycosylation states/CD45 glycoforms at each stage of B cell differentiation.
34962767	9	76	gly	O-glycosite	1398:1408	arg2	an O-glycosite			an O-glycosite						O-glycosite	Glycopeptides with as few as two amino acids on either side of an O-glycosite were cleaved by IMPa.
30850477	11	24	gly	N-glycosylation	1468:1482	arg2	N-glycosylation site acquisition			N-glycosylation site acquisition						site	These results point toward a differential selection pressure of N-glycosylation site acquisition during affinity maturation of B cells, which depends on the location within the V region and is isotype and subclass dependent.
30029404	7	29	gly	glycoproteins	1331:1343	arg1	78 glycoproteins	78 glycoproteins				Fterm		glycoproteins			In total, 207 glycopeptides assigned to 78 glycoproteins were identified from 2 μL human serum.
30029404	7	31	gly	glycopeptides	1302:1314	arg2	207 glycopeptides			207 glycopeptides						glycopeptides	In total, 207 glycopeptides assigned to 78 glycoproteins were identified from 2 μL human serum.
28929344	1	86	gly	glycoproteins	243:255	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			PURPOSE Glucosidase II plays a major role in regulating the post-translational modification of N-linked glycoproteins.
33340519	4	26	gly	glycosylation	609:621	arg1	ACE2	ACE2				OGER		ACE2	Q9BYF1		We therefore sought to investigate whether the glycosylation state of ACE2 impacts the interaction with SARS-CoV-2 viral spike.
28031460	4	8	gly	β-galactoside	630:642	arg1	St6gal1	-galactoside α2,6-sialyltransferase-1			St6gal1	PUBTATOR		-galactoside α2,6-sialyltransferase-1	20440		We found that a gene encoding β-galactoside α2,6-sialyltransferase-1 (St6gal1), a key enzyme responsible for the biosynthesis of α2,6-linked sialic acid in N-linked glycans, was most down-regulated in VATs from obese mice.
30740857	16	51	gly	glycans	2120:2126	arg1	FVIII	FVIII			glycans	PUBTATOR		FVIII	P00451		Conclusions These findings suggest that CLEC4M is a novel clearance receptor that interacts with mannose-exposed glycans on FVIII in the presence or absence of VWF.
33347638	10	87	gly	O-glycosylated	1959:1972	arg1	O-glycosylated clusterin	O-glycosylated clusterin				PUBTATOR		clusterin	1191		Following this discovery, we constructed a Luminex-based assay to quantify O-glycosylated clusterin, in which total serum clusterin was first captured on anti-clusterin antibody-immobilized beads, and then clusterin-associated O-glycans were determined by the pair of biotin-MPA and streptavidin-phycoerythrin.
32663509	8	65	gly	UDP-GlcNAc	1701:1710	arg1	the form	form of B3GNT2			UDP-GlcNAc	PUBTATOR		form of B3GNT2	Q9NY97		The presence of a sink for UDP-GlcNAc in the form of B3GNT2 with no disposition may have also elevated the intracellular levels of this nucleotide as well as its downstream product, CMP-Neu5Ac.
29339411	8	68	gly	glycopeptide	1609:1620	arg2	protonated glycopeptide ions			protonated glycopeptide ions						glycopeptide	We also found that monosaccharide rearrangement occurred during collision-induced dissociation (CID) of protonated glycopeptide ions.
31308178	4	33	gly	site	830:833	arg1	N-linked glycans	VEGFR2		Asn-247 site	N-linked glycans			VEGFR2	3791	Asn-247 site	Here, using glycoside hydrolase and kinase assays and immunoprecipitation and MS-based analyses, we demonstrate that N-linked glycans at the Asn-247 site in VEGFR2 hinder VEGF ligand-mediated receptor activation and signaling in endothelial cells.
35518855	8	7	gly	fucosylated	1225:1235	arg1	10 and 15 fucosylated N-linked glycans				10 and 15 fucosylated N-linked glycans						There were 10 and 15 fucosylated N-linked glycans that were detected in PCTs and LUSC tissues respectively, 10 fucosylated N-glycans were common, while five fucosylated N-glycans were unique to LUSC tissues.
35518855	8	40	gly	fucosylated	1315:1325	arg1	10 fucosylated N-glycans				10 fucosylated N-glycans						There were 10 and 15 fucosylated N-linked glycans that were detected in PCTs and LUSC tissues respectively, 10 fucosylated N-glycans were common, while five fucosylated N-glycans were unique to LUSC tissues.
35518855	8	73	gly	fucosylated	1361:1371	arg1	five fucosylated N-glycans				five fucosylated N-glycans						There were 10 and 15 fucosylated N-linked glycans that were detected in PCTs and LUSC tissues respectively, 10 fucosylated N-glycans were common, while five fucosylated N-glycans were unique to LUSC tissues.
34944439	9	33	gly	fucosylated	1537:1547	arg1	fucosylated carbohydrates				fucosylated carbohydrates						Given the biological importance of fucosylated carbohydrates, these FucTs are promising candidates for synthetic glycobiology.
32767558	3	30	gly	glycosylation	488:500	arg1	pea seed legumin	pea seed legumin				Fterm		legumin			RESULTS In this study, the effect of transglutaminase-induced oligochitosan glycosylation on the structural and functional properties of pea seed legumin was studied.
34010560	4	19	gly	glycosylation	1019:1031	arg2	glycosylation sites			glycosylation sites						sites	Herein, we present the software GlycoHybridSeq for automated interpretation of EThcD-MS/MS spectra from glycoproteomic data using a customized scoring function, which enables the functionalities of identifying glycopeptides, characterizing glycosylation sites, and distinguishing some isomeric glycans.
34010560	4	67	gly	glycopeptides	989:1001	arg2	glycopeptides			glycopeptides						glycopeptides	Herein, we present the software GlycoHybridSeq for automated interpretation of EThcD-MS/MS spectra from glycoproteomic data using a customized scoring function, which enables the functionalities of identifying glycopeptides, characterizing glycosylation sites, and distinguishing some isomeric glycans.
31019513	10	50	gly	glycosylation	1675:1687	arg2	these five glycosylation sites			these five glycosylation sites						sites	We show that mutation of these five glycosylation sites increased the clustering tendency of CD22 and resulted in higher density CD22 nanoclusters.
32782472	11	19	gly	position	2038:2045	arg1	glycans			position N45 and N384	glycans					position N45 and N384	Molecular dynamic simulation of the N-glycan mutants TrCel7A revealed higher number of contacts between CD and cellulose crystal upon removal of glycans at position N45 and N384.
32782472	11	83	gly	N384	2055:2058	arg1	glycans			position N45 and N384	glycans					position N45 and N384	Molecular dynamic simulation of the N-glycan mutants TrCel7A revealed higher number of contacts between CD and cellulose crystal upon removal of glycans at position N45 and N384.
33629527	2	59	gly	proteins	355:362	arg1	glycan patterns	proteins			glycan patterns	Fterm		proteins			In contrast, glycan patterns of immunogenic viral proteins, which differ significantly between the various expression systems, are hardly analyzed yet.
32366695	2	3	gly	glycoprotein	373:384	arg1	the spike (S) glycoprotein	the spike (S) glycoprotein				Fterm		glycoprotein			Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion.
33154157	1	37	gly	glycosylation	118:130	arg1	glycoprotein folding	glycoprotein folding				Fterm		glycoprotein			Mammalian protein N-linked glycosylation is critical for glycoprotein folding, quality control, trafficking, recognition, and function.
33154157	1	54	gly	glycoprotein	148:159	arg1	glycoprotein folding	glycoprotein folding				Fterm		glycoprotein			Mammalian protein N-linked glycosylation is critical for glycoprotein folding, quality control, trafficking, recognition, and function.
31616924	1	75	gly	O-glycoprotein	108:121	arg1	The O-glycoprotein apolipoprotein E	The O-glycoprotein apolipoprotein E				Fterm		O-glycoprotein			The O-glycoprotein apolipoprotein E (APOE), the strongest genetic risk factor for Alzheimer's disease, associates with lipoproteins.
28057501	6	9	gly	N-glycosylation	1116:1130	arg2	the most possible N-glycosylation sites			the most possible N-glycosylation sites						sites	Furthermore, PYC protein also had three potential N-linked glycosylation sites, among them, -N-S-T-I- at 36 amino acid, -N-G-T-V- at 237 amino acid, and -N-G-S-S- at 517 amino acid were the most possible N-glycosylation sites.
28057501	6	74	gly	glycosylation	971:983	arg2	three potential N-linked glycosylation sites			three potential N-linked glycosylation sites						sites	Furthermore, PYC protein also had three potential N-linked glycosylation sites, among them, -N-S-T-I- at 36 amino acid, -N-G-T-V- at 237 amino acid, and -N-G-S-S- at 517 amino acid were the most possible N-glycosylation sites.
34962767	11	53	gly	glycopeptide	1781:1792	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	We present the use of IMPa in a one-step O-glycoproteomic workflow for glycoprofiling of the purified glycoproteins granulocyte colony-stimulating factor and receptor-type tyrosine-protein phosphatase C without the need for glycopeptide enrichment.
34962767	11	84	gly	glycoproteins	1659:1671	arg1	the purified glycoproteins granulocyte colony-stimulating factor and receptor-type tyrosine-protein phosphatase C	the purified glycoproteins granulocyte colony-stimulating factor and receptor-type tyrosine-protein phosphatase C				Fterm		glycoproteins			We present the use of IMPa in a one-step O-glycoproteomic workflow for glycoprofiling of the purified glycoproteins granulocyte colony-stimulating factor and receptor-type tyrosine-protein phosphatase C without the need for glycopeptide enrichment.
34962767	11	84	gly	glycoproteins	1659:1671	arg1	granulocyte colony-stimulating factor	granulocyte colony-stimulating factor				OGER		granulocyte colony-stimulating factor	P09919		We present the use of IMPa in a one-step O-glycoproteomic workflow for glycoprofiling of the purified glycoproteins granulocyte colony-stimulating factor and receptor-type tyrosine-protein phosphatase C without the need for glycopeptide enrichment.
30834446	10	46	gly	glycoprotein	1827:1838	arg1	the IHNV glycoprotein	the IHNV glycoprotein				Fterm		glycoprotein			These models suggest mechanisms for which the binding of these galectins to the IHNV glycoprotein hinders with different potencies the viral attachment required for infection.
30042931	5	95	gly	E-glycosylation	928:942	arg1	both G1 and G2 sites			sites						sites	The G1G2 mutant (E-D67N), with E-glycosylation on both G1 and G2 sites, showed potent in vitro viral replication and in vivo neurovirulence, but reduced neuroinvasiveness.
32707739	3	36	gly	N-glycosylation	539:553	arg1	different AMPAR subunits	different AMPAR subunits				Fterm		subunits			Although N-glycosylation on different AMPAR subunits has been shown to regulate the ER-exit of hetero-oligomers, its role in the ER-exit of homo-oligomers remains unclear.
29784879	2	49	gly	glycoproteins	235:247	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Correct folding of glycoproteins in the endoplasmic reticulum (ER) is tightly monitored by the recognition of glycan signals by lectins in the ER-associated degradation (ERAD) pathway.
31527085	0	8	gly	O-GlcNAcylation	0:14	arg1	sOGT	sOGT			O-GlcNAcylation	Cterm		sOGT	8473		O-GlcNAcylation of Thr12/Ser56 in short-form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.
31527085	0	8	gly	O-GlcNAcylation	0:14	arg1	short-form O-GlcNAc transferase	transferase			O-GlcNAcylation	Fterm		transferase			O-GlcNAcylation of Thr12/Ser56 in short-form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.
34213308	5	41	gly	glycosylated	794:805	arg1	a fully glycosylated spike	a fully glycosylated spike				PUBTATOR		spike	43740568		Molecular dynamics simulations of a fully glycosylated spike support a model of steric restrictions that shape enzymatic processing of the glycans.
34106099	9	3	gly	sialylated	1592:1601	arg1	intact sialylated N-linked glycopeptides			intact sialylated N-linked glycopeptides						glycopeptides	However, correct identification of intact sialylated N-linked glycopeptides using the Byonic program failed, most likely due to the lack of peptide backbone fragmentation during tandem mass spectrometry.
34106099	9	44	gly	glycopeptides	1612:1624	arg2	intact sialylated N-linked glycopeptides			intact sialylated N-linked glycopeptides						glycopeptides	However, correct identification of intact sialylated N-linked glycopeptides using the Byonic program failed, most likely due to the lack of peptide backbone fragmentation during tandem mass spectrometry.
29681862	12	107	gly	eNOS	2313:2316	arg1	O-GlcNAcylation	eNOS			O-GlcNAcylation	PUBTATOR		eNOS	24600		These data indicate that O-GlcNAcylation contributes to metabolic syndrome-induced PVAT dysfunction and that O-GlcNAcylation of eNOS may be targeted in the development of novel therapies for vascular dysfunction in conditions associated with hyperglycemia.
32719555	0	52	gly	glycoprotein	38:49	arg1	orthogonal human O-linked glycoprotein biosynthesis	orthogonal human O-linked glycoprotein biosynthesis				Fterm		glycoprotein			Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria.
32461612	3	43	gly	glycosylation	466:478	arg2	66-87 N-linked glycosylation sites			66-87 N-linked glycosylation sites						sites	Coronavirus S proteins are extensively glycosylated, encoding around 66-87 N-linked glycosylation sites per trimeric spike.
32461612	3	65	gly	glycosylated	421:432	arg1	Coronavirus S proteins	Coronavirus S proteins				Cterm		Coronavirus S proteins	43740568		Coronavirus S proteins are extensively glycosylated, encoding around 66-87 N-linked glycosylation sites per trimeric spike.
30205382	8	64	gly	modified	1476:1483	arg1	STAT3 AND O-GlcNAcylation	STAT3			O-GlcNAcylation	PUBTATOR		STAT3	25125		Immunoprecipitation revealed that STAT3 was modified by O-GlcNAcylation and phosphorylation simultaneously.
32535395	13	19	gly	glycoproteins	1997:2009	arg1	epithelial secretory glycoproteins	epithelial secretory glycoproteins				Fterm		glycoproteins			We speculate that, as well as providing nourishment for the developing embryo, epithelial secretory glycoproteins may contribute components to the capsule, which develops only partially in embryos cultured in vitro.
30659065	11	84	gly	sites	1846:1850	arg1	N272	IgM		sites N46, N209, and N272				IgM	P01871	sites N46, N209, and N272	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	84	gly	sites	1846:1850	arg1	N46	IgM		sites N46, N209, and N272				IgM	P01871	sites N46, N209, and N272	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	84	gly	sites	1846:1850	arg1	IgM sites	IgM		sites N46, N209, and N272				IgM	P01871	sites N46, N209, and N272	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	84	gly	sites	1846:1850	arg1	N46	IgM		sites N46, N209, and N272				IgM	P01871	sites N46, N209, and N272	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	84	gly	sites	1846:1850	arg1	IgM sites	IgM		sites N46, N209, and N272				IgM	P01871	sites N46, N209, and N272	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	84	gly	sites	1846:1850	arg1	IgM sites	IgM		sites N46, N209, and N272				IgM	P01871	sites N46, N209, and N272	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	97	gly	sites	1914:1918	arg1	sites N279 and N439	IgM		sites N279 and N439				IgM	P01871	sites N279 and N439	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	97	gly	sites	1914:1918	arg1	N439	IgM		sites N279 and N439				IgM	P01871	sites N279 and N439	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
30659065	11	97	gly	sites	1914:1918	arg1	N439	IgM		sites N279 and N439				IgM	P01871	sites N279 and N439	IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms.
29267884	4	32	gly	glycosylation	729:741	arg2	sites			sites						sites	The initial data comprises sites of glycosylation for N-linked, O-GalNAc, O-Fucose, O-Xyl, O-Mannose (in both human and yeast) and cytosolic O-GlcNAc type.
34229070	6	22	gly	O-glycosites	910:921	arg2	14 O-glycosites			14 O-glycosites						O-glycosites	RESULTS A total of 14 O-glycosites were identified on three glycopeptides of APP, and at least four O-glycans including GalNAc (Tn antigen), core 1, and mono-/di-sialylated core 1 glycans were determinant at the residues of Thr576 and Thr577.
34229070	6	39	gly	mono-/di-sialylated	1041:1059	arg1	mono-/di-sialylated core 1 glycans				mono-/di-sialylated core 1 glycans						RESULTS A total of 14 O-glycosites were identified on three glycopeptides of APP, and at least four O-glycans including GalNAc (Tn antigen), core 1, and mono-/di-sialylated core 1 glycans were determinant at the residues of Thr576 and Thr577.
34229070	6	43	gly	residues	1100:1107	arg1	determinant			residues	determinant					residues	RESULTS A total of 14 O-glycosites were identified on three glycopeptides of APP, and at least four O-glycans including GalNAc (Tn antigen), core 1, and mono-/di-sialylated core 1 glycans were determinant at the residues of Thr576 and Thr577.
34229070	6	1	gly	glycopeptides	948:960	arg2	three glycopeptides	APP		glycopeptides		OGER		APP	P05067	glycopeptides	RESULTS A total of 14 O-glycosites were identified on three glycopeptides of APP, and at least four O-glycans including GalNAc (Tn antigen), core 1, and mono-/di-sialylated core 1 glycans were determinant at the residues of Thr576 and Thr577.
29526322	9	24	gly	de-glycosylated	1255:1269	arg1	de-glycosylated NCX3	de-glycosylated NCX3				PUBTATOR		NCX3	6547		This was accompanied by accumulation of de-glycosylated NCX3 in the cytosol (that is in the ER), where it transported calcium ions (Ca2+) from the cytosol to the ER.
29038508	6	49	gly	glycosylation	958:970	arg1	Fz8	Fz8				PUBTATOR		Fz8	Q9H461		OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8.
31826991	11	151	gly	presence	1481:1488	arg1	the head region AND high-mannose glycan			the head region	high-mannose glycan					region	Since SP-D affinity for influenza HA depends on the presence of high-mannose glycan on the head region, our data demonstrate that SP-D may not protect against virus containing these HA subtypes.
32827291	2	9	gly	glycosylation	405:417	arg2	two asparagine-linked glycosylation sites			two asparagine-linked glycosylation sites						sites	Herein, we identify two asparagine-linked glycosylation sites in B4GalT4.
32827291	2	49	gly	sites	419:423	arg1	B4GalT4			sites	B4GalT4					sites	Herein, we identify two asparagine-linked glycosylation sites in B4GalT4.
32452486	3	2	gly	glycopeptides	412:424	arg2	glycopeptides			glycopeptides						glycopeptides	Current technologies for glycan structural analysis normally involve the isolation of glycans from proteins, or enrichment of glycopeptides, and detection by mass spectrometry, which requires relatively large amounts of sample and is not able to be used by non-specialist laboratories.
32452486	3	23	gly	proteins	385:392	arg1	glycans	proteins			glycans	Fterm		proteins			Current technologies for glycan structural analysis normally involve the isolation of glycans from proteins, or enrichment of glycopeptides, and detection by mass spectrometry, which requires relatively large amounts of sample and is not able to be used by non-specialist laboratories.
34662441	6	65	gly	presence	1239:1246	arg2	the former sequons AND high mannose glycoforms			the former sequons	high mannose glycoforms						The presence of high mannose glycoforms at the former sequons likely results from the limited access of enzymes of the glycosynthetic pathway required for the synthesis of the complex structures.
34662441	6	80	gly	glycoforms	1264:1273	arg1	high mannose glycoforms				high mannose glycoforms						The presence of high mannose glycoforms at the former sequons likely results from the limited access of enzymes of the glycosynthetic pathway required for the synthesis of the complex structures.
29263266	3	70	gly	glycosylated	578:589	arg1	gp41	gp41				Cterm		gp41			Unlike the MBLs, which block HIV only through binding to the glycosylated envelope proteins (gp120 and gp41) of the virus, SBL inhibited HIV at multiple steps of the virus infection/replication cycle.
29263266	3	70	gly	glycosylated	578:589	arg1	gp120	gp120				OGER		gp120	Q14624		Unlike the MBLs, which block HIV only through binding to the glycosylated envelope proteins (gp120 and gp41) of the virus, SBL inhibited HIV at multiple steps of the virus infection/replication cycle.
29263266	3	70	gly	glycosylated	578:589	arg1	the glycosylated envelope proteins	the glycosylated envelope proteins				Fterm		proteins			Unlike the MBLs, which block HIV only through binding to the glycosylated envelope proteins (gp120 and gp41) of the virus, SBL inhibited HIV at multiple steps of the virus infection/replication cycle.
32915505	1	12	gly	glycoprotein	230:241	arg1	the SARS-CoV2 spike glycoprotein	the SARS-CoV2 spike glycoprotein				Fterm		glycoprotein			The glycan structures of the receptor binding domain of the SARS-CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR.
32915505	1	29	gly	domain	200:205	arg1	The glycan structures			domain	The glycan structures					domain	The glycan structures of the receptor binding domain of the SARS-CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR.
29295913	10	31	gly	O-mannosylation	1627:1641	arg1	proteins	proteins				Fterm		proteins			These findings demonstrate that O-mannosylation of proteins increases the thermotolerance of Histoplasma yeast cells, which facilitates infection of mammalian hosts.IMPORTANCE For dimorphic fungal pathogens, mammalian body temperature can have contrasting roles.
29295913	10	52	gly	proteins	1646:1653	arg1	O-mannosylation	proteins			O-mannosylation	Fterm		proteins			These findings demonstrate that O-mannosylation of proteins increases the thermotolerance of Histoplasma yeast cells, which facilitates infection of mammalian hosts.IMPORTANCE For dimorphic fungal pathogens, mammalian body temperature can have contrasting roles.
32699088	3	15	gly	glycosylation	517:529	arg2	three N-linked glycosylation sites			three N-linked glycosylation sites						sites	Sequence analysis showed that in the NA head domain of H1N1 IAVs, three N-linked glycosylation sites are conserved and that a fourth site is conserved in H3N2 IAVs.
32827291	0	35	gly	N-glycosylation	0:14	arg1	the human β1,4-galactosyltransferase 4	the human β1,4-galactosyltransferase 4				PUBTATOR		1,4-galactosyltransferase 4	8702		N-glycosylation of the human β1,4-galactosyltransferase 4 is crucial for its activity and Golgi localization.
33205023	3	37	gly	N-glycosylation	461:475	arg2	key N-glycosylation sites			key N-glycosylation sites						sites	Here, analysis of two Env variants from the same donor, with differing functional characteristics and N-glycosylation-site composition, revealed that changes to key N-glycosylation sites affected the Env structure at distant locations and had a ripple effect on Env-wide glycan processing, virus infectivity, antibody recognition, and virus neutralization.
31199881	3	115	gly	glycosylation	623:635	arg2	the Fc N-linked glycosylation site			the Fc N-linked glycosylation site						site	TfRMAbs have Fc effector function side effects, and removal of the Fc N-linked glycosylation site by substituting Asn with Gly reduces the Fc effector function.
30684504	4	24	gly	glycosylated	941:952	arg1	its N-linked glycosylated form				its N-linked glycosylated form						In its N-linked glycosylated form, the resulting fusion protein was easily purified from the culture supernatant via a three-step chromatography process.
30814666	0	46	gly	glycosylation	6:18	arg1	cell envelope proteins	cell envelope proteins				Fterm		proteins			Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex.
32782472	6	102	gly	N-glycosylation	1115:1129	arg2	N-glycosylation site			N-glycosylation site						site	Also, we expressed single N-glycosylation site deficient mutants TrCel7A (N45Q , N270Q , N384Q ).
30775959	7	66	gly	AF10T	846:850	arg1	carbohydrate active enzymes	AF10T			carbohydrate active enzymes	Cterm		AF10T			The genome of strain AF10T was 6.14 Mbp in size and encoded a wide repertoire of carbohydrate active enzymes.
30023808	3	80	gly	glycopeptides	655:667	arg2	N-linked glycopeptides			N-linked glycopeptides						glycopeptides	The obtained superhydrophilic Fe3O4-PEI-pMaltose NPs displayed outstanding advantages in the enrichment of N-linked glycopeptides, including high selectivity (1:100, mass ratios of HRP and bovine serum albumin (BSA) digest), low detection limit (10 fmol), large binding capacity (200 mg/g), and high enrichment recovery (above 85%).
31178836	5	28	gly	glycan	1004:1009	arg1	gp160	gp160			glycan	PUBTATOR		gp160	2028		Shorter V1 length, less potential glycan and a lower ratio of NXT: NXS in gp160 were observed in the first five time points compared to that from the last time points, as well that from the Chinese B_database.
31178836	5	99	gly	NXT	1032:1034	arg1	less potential glycan	NXT			less potential glycan	PUBTATOR		NXT	29107		Shorter V1 length, less potential glycan and a lower ratio of NXT: NXS in gp160 were observed in the first five time points compared to that from the last time points, as well that from the Chinese B_database.
29596379	4	51	gly	glycosylation	750:762	arg2	three N-linked glycosylation sites			three N-linked glycosylation sites						sites	The deduced protein (maNRAMP) possesses the typical structural features of NRAMP protein family, including 12 transmembrane domains, three N-linked glycosylation sites, and a conserved transport motif.
31059811	5	112	gly	leucine-rich	746:757	arg1	eleven leucine-rich repeats			leucine	eleven leucine-rich repeats					leucine	The derived amino acids sequence comprised of an extracellular domain including 26 amino acids signal peptide and eleven leucine-rich repeats (LRR), capped with LRRCT and LRRNT followed by transmembrane domain and cytoplasmic Toll/IL-1R domain (TIR).
28067406	3	31	gly	N-glycosylation	484:498	arg2	Asn60			Asn60				Ninjurin1 Assembles	4814	Asn60	We showed that Ninj1 assembles into a homomeric complex via a cis-interaction mediated by the intracellular region and N-glycosylation at Asn60 .
30101988	0	65	gly	N-glycosylation	125:139	arg1	human immunoglobulin G1			human immunoglobulin G1						G1" by J.J. Bevelacqua and S.M.	Authors' Reply to the Commentary in the journal of Electrophoresis regarding "The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1" by J.J. Bevelacqua and S.M.J. Mortazavi.
31231989	10	37	gly	transferrin	1456:1466	arg1	the N-linked glycans	transferrin			the N-linked glycans	OGER		transferrin	P02787		Partial loss of galactose and sialic acid of the N-linked glycans of serum transferrin was observed.
30712568	4	66	gly	glycopeptides	911:923	arg2	glycopeptides			glycopeptides						glycopeptides	The well-designed composites presented clearly ZIC-polymer shell and superior hydrophilicity (water contact angle 30.2), and the performance for selective enrichment of glycopeptides were investigated with standard and real samples, respectively.
29980609	8	71	gly	glycosylation	1599:1611	arg2	the atypical glycosylation site			the atypical glycosylation site						site	However, although the extent of conventional N-linked glycosylation positively correlates with AICL surface expression, the atypical glycosylation site impairs AICL surfacing.
30881698	10	15	gly	deglycosylate	1417:1429	arg1	whey proteins	whey proteins				Fterm		proteins			In view of future applications in the dairy industry, we investigated the ability of this enzyme to deglycosylate whey proteins.
29681862	4	41	gly	proteins	842:849	arg1	tissue-specific O-GlcNAc modification	proteins			tissue-specific O-GlcNAc modification	Fterm		proteins			Increased flux of glucose through the hexosamine biosynthetic pathway and the consequent increase in tissue-specific O-GlcNAc modification of proteins have been linked to multiple facets of vascular dysfunction in diabetes and other pathological conditions.
29681862	4	60	gly	modification	826:837	arg3	proteins AND tissue-specific O-GlcNAc modification	proteins			tissue-specific O-GlcNAc modification	Fterm		proteins			Increased flux of glucose through the hexosamine biosynthetic pathway and the consequent increase in tissue-specific O-GlcNAc modification of proteins have been linked to multiple facets of vascular dysfunction in diabetes and other pathological conditions.
33273015	4	83	gly	carries	484:490	arg1	nMPO AND both characteristic under-processed and hyper-truncated glycans	nMPO			both characteristic under-processed and hyper-truncated glycans	Cterm		nMPO	P05164		Mass spectrometry demonstrated that nMPO carries both characteristic under-processed and hyper-truncated glycans.
30069741	1	15	gly	glycosylated	94:105	arg1	Most secreted proteins	Most secreted proteins				Fterm		proteins			Most secreted proteins in eukaryotes are glycosylated, and after a number of common biosynthesis steps the glycan structures mature in a species-dependent manner.
29409894	2	68	gly	glycoproteins	329:341	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The maturation of glycoproteins involves the trimming of mannosyl residues by mannosidases and addition of other sugar molecules to three-branched N-glycans in the Golgi.
30944176	11	21	gly	glycosylated	1930:1941	arg1	a viral envelope protein	a viral envelope protein				PUBTATOR		envelope protein	64006		All isolates from recent outbreaks encode a viral envelope protein that is glycosylated, whereas many historical ZIKV strains lack this glycosylation.
31196789	7	46	gly	glycosylation	974:986	arg1	N454			N313 and N454				Lcc9		N313 and N454	Comparison of catalytic properties of those mutants revealed that glycosylation at N313 and N454 in rLcc9 could affect the binding affinity to substrates and the catalytic rate, respectively.
31196789	7	46	gly	glycosylation	974:986	arg2	N454			N313 and N454				Lcc9		N313 and N454	Comparison of catalytic properties of those mutants revealed that glycosylation at N313 and N454 in rLcc9 could affect the binding affinity to substrates and the catalytic rate, respectively.
31196789	7	46	gly	glycosylation	974:986	arg1	N313			N313 and N454				Lcc9		N313 and N454	Comparison of catalytic properties of those mutants revealed that glycosylation at N313 and N454 in rLcc9 could affect the binding affinity to substrates and the catalytic rate, respectively.
31196789	7	46	gly	glycosylation	974:986	arg2	N454			N313 and N454				Lcc9		N313 and N454	Comparison of catalytic properties of those mutants revealed that glycosylation at N313 and N454 in rLcc9 could affect the binding affinity to substrates and the catalytic rate, respectively.
31196789	7	46	gly	glycosylation	974:986	arg1	N313			N313 and N454				Lcc9		N313 and N454	Comparison of catalytic properties of those mutants revealed that glycosylation at N313 and N454 in rLcc9 could affect the binding affinity to substrates and the catalytic rate, respectively.
31196789	7	46	gly	glycosylation	974:986	arg1	N313			N313 and N454				Lcc9		N313 and N454	Comparison of catalytic properties of those mutants revealed that glycosylation at N313 and N454 in rLcc9 could affect the binding affinity to substrates and the catalytic rate, respectively.
33677548	3	12	gly	glycoproteins	490:502	arg1	glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			One of the major reasons is the absence of digital standard to represent glycoproteins and the challenging annotations with glycans.
33300777	6	64	gly	disialylated	1137:1148	arg1	the biantennary digalactosylated disialylated N-glycan A2G2S2				the biantennary digalactosylated disialylated N-glycan A2G2S2						As proof of the principle, the biantennary digalactosylated disialylated N-glycan A2G2S2, against which there have been no antibodies and lectins so far, was employed as the target.
31471298	9	4	gly	sites	1648:1652	arg1	V-domain glycans			sites	V-domain glycans					sites	CONCLUSION Our observations indicate that somatic hypermutation of ACPA, which results in the incorporation of N-linked glycosylation sites and consequently V-domain glycans, occurs already years before symptom onset in individuals that will develop RA later in life.
31471298	9	99	gly	glycosylation	1634:1646	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	CONCLUSION Our observations indicate that somatic hypermutation of ACPA, which results in the incorporation of N-linked glycosylation sites and consequently V-domain glycans, occurs already years before symptom onset in individuals that will develop RA later in life.
30250045	9	78	gly	contained	1331:1339	arg1	the epitope AND a similar glycan modification			the epitope	a similar glycan modification					epitope	Neuraminidase treatment analysis also confirmed that the epitope in mesothelioma cells contained a similar glycan modification.
31636418	8	4	gly	found	1271:1275	arg1	exposed positions AND N-linked glycans			exposed positions	N-linked glycans					positions	In contrast, polymorphic sites and N-linked glycans are preferentially found in exposed positions on the receptor surface, not contacting transferrin, suggesting that transferrin receptor diversification is driven by a need for antigenic variation in the receptor to prolong survival in a host.
33676668	2	45	gly	glycopeptide	313:324	arg2	glycopeptide			glycopeptide						glycopeptide	However, due to the diversity of glycopeptide forms and their low abundance, it is extraordinarily challenging to capture and separate glycopeptides with high selectivity from complex biological samples with mass spectrometric analysis.
33676668	2	77	gly	glycopeptides	415:427	arg2	capture and separate glycopeptides			capture and separate glycopeptides						glycopeptides	However, due to the diversity of glycopeptide forms and their low abundance, it is extraordinarily challenging to capture and separate glycopeptides with high selectivity from complex biological samples with mass spectrometric analysis.
29784879	7	24	gly	glycoprotein	1335:1346	arg1	the glycoprotein T-cell receptor α locus	the glycoprotein T-cell receptor α locus				Fterm		glycoprotein			In a defined in vitro system consisting of recombinant proteins purified from HEK293 cells, the mannose-trimming activity of EDEM3 toward the model misfolded substrate, the glycoprotein T-cell receptor α locus (TCRα), was reconstituted only when ERp46 had established a covalent interaction with EDEM3.
29784879	7	64	gly	EDEM3	1287:1291	arg1	the mannose-trimming activity	EDEM3			the mannose-trimming activity	PUBTATOR		EDEM3	80267		In a defined in vitro system consisting of recombinant proteins purified from HEK293 cells, the mannose-trimming activity of EDEM3 toward the model misfolded substrate, the glycoprotein T-cell receptor α locus (TCRα), was reconstituted only when ERp46 had established a covalent interaction with EDEM3.
32236654	8	87	gly	sialylated	1456:1465	arg1	truncated α2,6-core sialylated glycans				truncated α2,6-core sialylated glycans						Colon-like cell lines are characterized by a prevalence of I-branched and sialyl Lewis x/a epitope carrying glycans, while most undifferentiated cell lines show absence of Lewis epitope expression resulting in dominance of truncated α2,6-core sialylated glycans.
32236654	8	31	gly	carrying	1312:1319	arg1	I-branched and sialyl Lewis x/a epitope AND glycans			I-branched and sialyl Lewis x/a epitope	glycans						Colon-like cell lines are characterized by a prevalence of I-branched and sialyl Lewis x/a epitope carrying glycans, while most undifferentiated cell lines show absence of Lewis epitope expression resulting in dominance of truncated α2,6-core sialylated glycans.
33600485	9	33	gly	glycosylation	1179:1191	arg2	both glycosylation sites			both glycosylation sites						sites	A total of 100 structures were detected on both glycosylation sites.
31471298	2	31	gly	N-glycosylation	378:392	arg2	N-glycosylation sites			N-glycosylation sites						sites	Recently, we showed that N-glycosylation sites, which are required for the incorporation of V-domain glycans, are introduced following somatic hypermutation.
29541627	6	57	gly	N-glycosylation	1742:1756	arg1	preferred and non-preferred sites			sites						sites	The current genome-wide mapping of 1,117 proteins (2,909 asparagine residues) was used to explore charge- and polarity-based mechanistic constraints in N-glycosylation, and discuss alterations of the neoplastic phenotype that can be ascribed to N-glycosylation at preferred and non-preferred sites.
30633504	5	27	gly	N-glycosylation	832:846	arg2	any position			position						position	We found that the N-glycosylation of RNase 1 at any position attenuates its catalytic activity but enhances both its thermostability and its resistance to proteolysis.
30633504	5	27	gly	N-glycosylation	832:846	arg1	RNase 1	RNase 1		position		PUBTATOR		RNase 1	6035	position	We found that the N-glycosylation of RNase 1 at any position attenuates its catalytic activity but enhances both its thermostability and its resistance to proteolysis.
30633504	5	27	gly	N-glycosylation	832:846	arg1	RNase 1	RNase 1		position		PUBTATOR		RNase 1	6035	position	We found that the N-glycosylation of RNase 1 at any position attenuates its catalytic activity but enhances both its thermostability and its resistance to proteolysis.
33093196	1	54	gly	glycans	88:94	arg1	the HIV-1 envelope glycoprotein	glycoprotein			glycans	Fterm		glycoprotein			The dense array of N-linked glycans on the HIV-1 envelope glycoprotein (Env), known as the "glycan shield," is a key determinant of immunogenicity, yet intrinsic heterogeneity confounds typical structure-function analysis.
33093196	1	47	gly	glycoprotein	118:129	arg1	the HIV-1 envelope glycoprotein	glycoprotein			a key determinant	Fterm		glycoprotein			The dense array of N-linked glycans on the HIV-1 envelope glycoprotein (Env), known as the "glycan shield," is a key determinant of immunogenicity, yet intrinsic heterogeneity confounds typical structure-function analysis.
29988048	3	50	gly	glycoprotein	339:350	arg1	its variant surface glycoprotein	its variant surface glycoprotein				Fterm		glycoprotein			The parasite elicits robust antibody-mediated immune responses to its variant surface glycoprotein (VSG) coat, but evades immune clearance by repeatedly accessing a large genetic VSG repertoire and 'switching' to antigenically distinct VSGs.
34551980	0	28	gly	neoglycoproteins	103:118	arg1	neoglycoproteins	neoglycoproteins				Fterm		neoglycoproteins			Shotgun scanning glycomutagenesis: A simple and efficient strategy for constructing and characterizing neoglycoproteins.
33676668	5	7	gly	glycopeptides	1173:1185	arg2	glycopeptides			glycopeptides						glycopeptides	Thirty-two glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were observed with a greatly improved signal-to-noise ratio (S/N) and also presented high performance in anti-interfering enrichment of glycopeptides from complex samples containing 100-fold bovine serum albumin tryptic digests.
33676668	5	13	gly	glycopeptides	967:979	arg2	Thirty-two glycopeptides			Thirty-two glycopeptides						glycopeptides	Thirty-two glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were observed with a greatly improved signal-to-noise ratio (S/N) and also presented high performance in anti-interfering enrichment of glycopeptides from complex samples containing 100-fold bovine serum albumin tryptic digests.
33525794	3	41	gly	glycoprotein	631:642	arg1	efficient glycoprotein expression platforms	efficient glycoprotein expression platforms				Fterm		glycoprotein			It is becoming clear that future progress in creating efficient glycoprotein expression platforms in bacteria will depend on the adoption of advanced strain engineering strategies such as rational design and assembly of orthogonal glycosylation pathways, genome-wide identification of metabolic engineering targets, and evolutionary engineering of pathway performance.
31826991	6	54	gly	glycosylation	943:955	arg2	each head glycosylation site			each head glycosylation site						site	Using mass spectrometry, we determined the glycan subclasses and heterogeneities at each head glycosylation site.
34200965	8	10	gly	glycoproteins	1191:1203	arg1	the altered glycoproteins	the altered glycoproteins				Fterm		glycoproteins			N-glycosidase treatment combined with nanoUPLC-coupled mass spectrometry identified the altered glycoproteins and respective glycosites.
34200965	8	24	gly	glycosites	1220:1229	arg2	respective glycosites			respective glycosites						glycosites	N-glycosidase treatment combined with nanoUPLC-coupled mass spectrometry identified the altered glycoproteins and respective glycosites.
29805493	6	22	gly	SCP	653:655	arg1	The monosaccharide composition	SCP			The monosaccharide composition	OGER		SCP	Q9CXK4		The monosaccharide composition of SCP was determined by high-performance liquid chromatography.
32462590	2	45	gly	N-glycoproteins	382:396	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			However, this likely represents a narrow functional role for the diverse array of glycan structures currently associated with N-glycoproteins in plants.
32915358	5	31	gly	glycoproteins	1159:1171	arg1	a detailed N-glycan characterization	glycoproteins			a detailed N-glycan characterization	Fterm		glycoproteins			We exploited the analytical capabilities of RapiFluor-MS (RFMS) to perform, by a slightly modified protocol, a detailed N-glycan characterization of total serum and single serum glycoproteins from specific patients with CDG (MAN1B1-CDG, ALG12-CDG, MOGS-CDG, TMEM199-CDG).
33947960	4	21	gly	modified	862:869	arg3	ErbB2 AND both α2,6- and α2,3-sialylated glycan structures	ErbB2			both α2,6- and α2,3-sialylated glycan structures	PUBTATOR		ErbB2	2064		Here, we demonstrate that ErbB2 is modified with both α2,6- and α2,3-sialylated glycan structures in GC clinical specimens.
33947960	4	57	gly	α2,3-sialylated	891:905	arg1	both α2,6- and α2,3-sialylated glycan structures				both α2,6- and α2,3-sialylated glycan structures						Here, we demonstrate that ErbB2 is modified with both α2,6- and α2,3-sialylated glycan structures in GC clinical specimens.
29541627	5	87	gly	glycosylation	1306:1318	arg1	membrane proteins	membrane proteins				Fterm		proteins			However, the alteration in side-chain charge and polarity environment of genetic variants, particularly in the vicinity of Asn-containing epitope, may induce constitutive glycosylation (e.g., aberrant glycosylation at preferred and non-preferred sites) of membrane proteins causing constitutive proliferation and triggering epithelial-to-mesenchymal transition.
29541627	5	52	gly	glycosylation	1336:1348	arg2	preferred and non-preferred sites			sites						sites	However, the alteration in side-chain charge and polarity environment of genetic variants, particularly in the vicinity of Asn-containing epitope, may induce constitutive glycosylation (e.g., aberrant glycosylation at preferred and non-preferred sites) of membrane proteins causing constitutive proliferation and triggering epithelial-to-mesenchymal transition.
30591584	6	8	gly	proteins	1111:1118	arg1	O-GalNAc glycosylation	proteins			O-GalNAc glycosylation	Fterm		proteins			The demonstration of O-GalNAc glycosylation of nuclear proteins in mammalian cells reported here has important implications for cell and chemical biology.
30591584	6	50	gly	glycosylation	1086:1098	arg1	nuclear proteins	nuclear proteins				Fterm		proteins			The demonstration of O-GalNAc glycosylation of nuclear proteins in mammalian cells reported here has important implications for cell and chemical biology.
34358619	2	87	gly	attached	269:276	arg2	cell membrane proteins AND N-glycans	cell membrane proteins			N-glycans	Fterm		proteins			N-glycans attached to cell membrane proteins play crucial roles in all aspects of biology, including embryogenesis, development, cell-cell recognition and adhesion, and cell signaling and communication.
30991145	4	40	gly	glycosylation	795:807	arg2	four N-linked glycosylation sites			four N-linked glycosylation sites						sites	In this study, we predicted four N-linked glycosylation sites at position 56, 379, 401, and 438 Asp (N) in G protein, and using a reverse genetics system developed in our laboratory, constructed nine recombinant viruses with single, triple, or quadruple glycosylation site disruptions using alanine substitutions in the following combinations: rIHNV-N56A, rIHNV-N379A, rIHNV-N401A, rIHNV-N438A, rIHNV-N56A-N379A-N401A, rIHNV-N56A-N379A-N438A, rIHNV-N56A-N401A-N438A, rIHNV-N379A-N401A-N438A, and rIHNV-N56A-N379A-N401A-N438A.
30991145	4	74	gly	glycosylation	1007:1019	arg2	quadruple glycosylation site disruptions			quadruple glycosylation site disruptions						site	In this study, we predicted four N-linked glycosylation sites at position 56, 379, 401, and 438 Asp (N) in G protein, and using a reverse genetics system developed in our laboratory, constructed nine recombinant viruses with single, triple, or quadruple glycosylation site disruptions using alanine substitutions in the following combinations: rIHNV-N56A, rIHNV-N379A, rIHNV-N401A, rIHNV-N438A, rIHNV-N56A-N379A-N401A, rIHNV-N56A-N379A-N438A, rIHNV-N56A-N401A-N438A, rIHNV-N379A-N401A-N438A, and rIHNV-N56A-N379A-N401A-N438A.
31866443	8	18	gly	proteins	1126:1133	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins			Functional studies in human adipocytes established a mechanistic link between reduced glutamine, O-GlcNAcylation of nuclear proteins, and a pro-inflammatory transcriptional response.
30852271	0	40	gly	glycosylation	9:21	arg1	the membrane protein ectodomain			the membrane protein ectodomain						ectodomain	N-Linked glycosylation of the membrane protein ectodomain regulates infectious bronchitis virus-induced ER stress response, apoptosis and pathogenesis.
34450331	6	39	gly	glycoproteins	1135:1147	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Data from the LDA and 3D structure analysis showed that glycoproteins predominantly undergo structural changes in loops/turns upon ER stress as exemplified with detailed analysis of ephrin-A5, GALNT10, PVR and BCAM.
30834446	0	65	gly	glycoprotein	135:146	arg1	the infectious hematopoietic necrosis virus (IHNV) envelope glycoprotein	the infectious hematopoietic necrosis virus (IHNV) envelope glycoprotein				Fterm		glycoprotein			Structure of the zebrafish galectin-1-L2 and model of its interaction with the infectious hematopoietic necrosis virus (IHNV) envelope glycoprotein.
34523671	1	66	gly	O-glycosylation	231:245	arg1	therapeutic proteins	therapeutic proteins				Fterm		proteins			We have been developing bacterial expression systems for human mucin-type O-glycosylation on therapeutic proteins, which is initiated by the addition of α-linked GalNAc to serine or threonine residues by enzymes in the GT-27 family of glycosyltransferases.
34523671	1	90	gly	serine	329:334	arg1	residues			residues						threonine residues	We have been developing bacterial expression systems for human mucin-type O-glycosylation on therapeutic proteins, which is initiated by the addition of α-linked GalNAc to serine or threonine residues by enzymes in the GT-27 family of glycosyltransferases.
32364434	7	25	gly	released	1323:1330	arg1	glycoproteins AND The glycans	glycoproteins			The glycans	Fterm		glycoproteins			The glycans released from the plasma, cells, and glycoproteins are permethylated in microscale in a 96-well plate or microcentrifuge tube and isolated by a C18 tip-based cleanup through a shorter and simple process.
32364434	7	44	gly	glycoproteins	1360:1372	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The glycans released from the plasma, cells, and glycoproteins are permethylated in microscale in a 96-well plate or microcentrifuge tube and isolated by a C18 tip-based cleanup through a shorter and simple process.
33135055	6	0	gly	O-glycosylation	883:897	arg2	one novel O-glycosylation site			one novel O-glycosylation site						site	We observed high glycan occupancy (73.2 to 100%) at all seven possible N-glycosylation sites and surprisingly detected one novel O-glycosylation site.
33135055	6	18	gly	N-glycosylation	825:839	arg2	all seven possible N-glycosylation sites			all seven possible N-glycosylation sites						sites	We observed high glycan occupancy (73.2 to 100%) at all seven possible N-glycosylation sites and surprisingly detected one novel O-glycosylation site.
30487799	7	18	gly	site	1234:1237	arg1	Glycan variation			site	Glycan variation					site	Glycan variation at a single site was greatest at the Asn-262 glycosite.
30487799	7	83	gly	glycosite	1267:1275	arg2	the Asn-262 glycosite			the Asn-262 glycosite				Grass Carp Serum IgM	Q15327	Asn-262 glycosite	Glycan variation at a single site was greatest at the Asn-262 glycosite.
33039440	8	13	gly	deglycosylated	1214:1227	arg1	deglycosylated, hypo-galactosylated and hyper-galactosylated variants	deglycosylated, hypo-galactosylated and hyper-galactosylated variants				Fterm		variants			In this article, we report optimized lab-scale workflows and detailed protocols for generation of deglycosylated, hypo-galactosylated and hyper-galactosylated variants of IgG therapeutic antibodies using the in-vitro glycoengineering technology.
33092920	6	64	gly	trifucosylated	662:675	arg1	The triantennary trifucosylated complex N-glycan				The triantennary trifucosylated complex N-glycan						The triantennary trifucosylated complex N-glycan (H5N5F3) was reported for the first time.
34213308	3	8	gly	glycosylation	387:399	arg1	recombinant SARS-CoV-2 spike proteins	recombinant SARS-CoV-2 spike proteins				PUBTATOR		spike proteins	43740568		Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against S protein from infectious virus, cultured in Vero cells.
33629527	4	31	gly	glycoproteins	815:827	arg1	IAV glycoproteins	IAV glycoproteins				Fterm		glycoproteins			Here, we present a versatile and powerful glycoanalytical workflow that enables a comprehensive N-glycosylation analysis of IAV glycoproteins.
33629527	4	41	gly	N-glycosylation	783:797	arg1	IAV glycoproteins	IAV glycoproteins				Fterm		glycoproteins			Here, we present a versatile and powerful glycoanalytical workflow that enables a comprehensive N-glycosylation analysis of IAV glycoproteins.
30581149	11	82	gly	HHM-glycoproteins	1831:1847	arg1	CONCLUSION Defined recombinant HHM-glycoproteins	CONCLUSION Defined recombinant HHM-glycoproteins				OGER		HHM	O95273		CONCLUSION Defined recombinant HHM-glycoproteins resembling carbohydrate-specific IgE epitopes from plants, venoms and mites were engineered which made it possible to discriminate carbohydrate- from peptide-specific IgE reactivity.
30659065	1	17	gly	glycoproteins	166:178	arg1	critical glycoproteins	critical glycoproteins				Fterm		glycoproteins			Antibodies are critical glycoproteins that bridge the innate and adaptive immune systems to provide protection against infection.
30036038	7	7	gly	glycoproteins	1024:1036	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			With glycoproteins containing only N-glycans as well as both N- and O-glycans as glycan source, this approach was proved to be widely applicable and efficient.
30036038	7	43	gly	containing	1038:1047	arg1	glycoproteins AND N-	glycoproteins			N-	Fterm		glycoproteins			With glycoproteins containing only N-glycans as well as both N- and O-glycans as glycan source, this approach was proved to be widely applicable and efficient.
30036038	7	43	gly	containing	1038:1047	arg1	glycoproteins AND only N-glycans	glycoproteins			only N-glycans	Fterm		glycoproteins			With glycoproteins containing only N-glycans as well as both N- and O-glycans as glycan source, this approach was proved to be widely applicable and efficient.
28976803	5	4	gly	mannosylation	1182:1194	arg1	the virulence factor	the virulence factor				Fterm		factor			Phenotypic comparison of the arg1Δ and kcs1Δ deletion mutants (both PP-IP5-deficient) reveals that arg1Δ has the most deleterious phenotype: while PP-IP5 is essential for metabolic and stress adaptation in both mutant strains, PP-IP5 is dispensable for virulence-associated functions such as capsule production, cell wall organization, and normal N-linked mannosylation of the virulence factor, phospholipase B1, as these phenotypes were defective only in arg1Δ.
28976803	5	4	gly	mannosylation	1182:1194	arg1	phospholipase B1	phospholipase B1				PUBTATOR		phospholipase B1	665270		Phenotypic comparison of the arg1Δ and kcs1Δ deletion mutants (both PP-IP5-deficient) reveals that arg1Δ has the most deleterious phenotype: while PP-IP5 is essential for metabolic and stress adaptation in both mutant strains, PP-IP5 is dispensable for virulence-associated functions such as capsule production, cell wall organization, and normal N-linked mannosylation of the virulence factor, phospholipase B1, as these phenotypes were defective only in arg1Δ.
28976803	5	30	gly	factor	1213:1218	arg1	normal N-linked mannosylation	factor			normal N-linked mannosylation	Fterm		factor			Phenotypic comparison of the arg1Δ and kcs1Δ deletion mutants (both PP-IP5-deficient) reveals that arg1Δ has the most deleterious phenotype: while PP-IP5 is essential for metabolic and stress adaptation in both mutant strains, PP-IP5 is dispensable for virulence-associated functions such as capsule production, cell wall organization, and normal N-linked mannosylation of the virulence factor, phospholipase B1, as these phenotypes were defective only in arg1Δ.
32553552	5	15	gly	glycopeptides	746:758	arg2	the resulting glycopeptides			the resulting glycopeptides						glycopeptides	We subjected midgut cell microvilli proteins to proteolytic digestion and enriched the resulting glycopeptides prior to analysis.
31358982	3	0	gly	present	708:714	arg1	glycoproteins AND N-linked glycans	glycoproteins			N-linked glycans	Fterm		glycoproteins			We further show that recruited IDCs express the C-type lectin receptor SIGN-R1, which mediates direct recognition of the virus by interacting with N-linked glycans present in glycoproteins of the virion envelope.
31358982	3	23	gly	glycoproteins	719:731	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We further show that recruited IDCs express the C-type lectin receptor SIGN-R1, which mediates direct recognition of the virus by interacting with N-linked glycans present in glycoproteins of the virion envelope.
31511323	7	76	gly	nonglycosylated	1148:1162	arg1	The nonglycosylated variant	The nonglycosylated variant				Fterm		variant			The nonglycosylated variant reached the cell surface by bypassing the Golgi compartment at a rate of only approximately one-third of that of WT β2.
36303733	5	25	gly	protein	977:983	arg1	The N-glycan biosynthesis network	spike protein			The N-glycan biosynthesis network	PUBTATOR		spike protein	43740568		Results: The N-glycan biosynthesis network of SARS-CoV-2 spike protein shows an elaborate enzymatic pathway with several intermediate glycans, along with the ones identified by mass spectrometric studies.
34869209	5	11	gly	glycosylation	583:595	arg1	the HEK293 recombinant spike RBD and S1 domains	spike RBD		domains		PUBTATOR		spike RBD	43740568	domains	In this study, we have characterized the site-specific glycosylation patterns of the HEK293 recombinant spike RBD and S1 domains as well as the intact spike derived from the whole virus produced in Vero cells.
34869209	5	11	gly	glycosylation	583:595	arg1	the intact spike	spike		domains		PUBTATOR		spike	43740568	domains	In this study, we have characterized the site-specific glycosylation patterns of the HEK293 recombinant spike RBD and S1 domains as well as the intact spike derived from the whole virus produced in Vero cells.
30348809	6	24	gly	receptor	840:847	arg1	sialylation	receptor			sialylation	Fterm		receptor			N610 was also the primary site of sialylation of the receptor.
30348809	6	34	gly	sialylation	821:831	arg1	the receptor	the receptor				Fterm		receptor			N610 was also the primary site of sialylation of the receptor.
32269229	7	40	gly	N-glycosite	1780:1790	arg2	N-glycosite			N-glycosite						N-glycosite	Our results lead us to propose that unique primary structural compositions and differential propensities for evolvability allowed for the mutual spatial exclusion of N-glycosite neighborhoods and disordered stretches.
32817340	0	82	gly	glycan	35:40	arg1	S-layer protein	protein			glycan	Fterm		protein			Identification of a novel N-linked glycan on the archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus.
28528272	10	70	gly	unglycosylated	1575:1588	arg1	unglycosylated prolegumain	unglycosylated prolegumain				Fterm		prolegumain			Glycosylated prolegumain was internalized and processed to the mature active form, but no internalization of unglycosylated prolegumain was observed.
32374435	1	7	gly	glycoproteins	247:259	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			The glycosylation pathways of several eukaryotic protein expression hosts are being engineered to enable the production of therapeutic glycoproteins with humanized application-customized glycan structures.
32080177	6	34	gly	arm	966:968	arg1	the α1,3 arm GlcNAc				the α1,3 arm GlcNAc						An exosite, formed by one of these loops and an SH3 domain, is responsible for the recognition of branched sugars, making contacts specifically to the α1,3 arm GlcNAc, a feature required for catalysis.
33435207	7	57	gly	glycoproteins	1471:1483	arg1	their glycoproteins	their glycoproteins				Fterm		glycoproteins			Importantly, these findings provide additional support that the chloroviruses do not use the canonical host endoplasmic reticulum-Golgi glycosylation pathway to glycosylate their glycoproteins; instead, they perform glycosylation independent of cellular organelles using virus-encoded enzymes.
33435207	7	63	gly	glycosylate	1453:1463	arg1	their glycoproteins	their glycoproteins				Fterm		glycoproteins			Importantly, these findings provide additional support that the chloroviruses do not use the canonical host endoplasmic reticulum-Golgi glycosylation pathway to glycosylate their glycoproteins; instead, they perform glycosylation independent of cellular organelles using virus-encoded enzymes.
29980609	4	30	gly	glycoproteins	755:767	arg1	molecular determinants	AICL glycoproteins			molecular determinants	PUBTATOR		AICL glycoproteins	9976		In this study, we characterize molecular determinants of AICL glycoproteins that cause intracellular retention, thereby controlling AICL surface expression.
33581334	6	57	gly	O-glycosylation	745:759	arg2	the recorded O-glycosylation sites			the recorded O-glycosylation sites						sites	Based on the recorded O-glycosylation sites, an O-glycosylation site prediction tool was developed.
33581334	6	71	gly	O-glycosylation	771:785	arg2	an O-glycosylation site prediction tool			an O-glycosylation site prediction tool						site	Based on the recorded O-glycosylation sites, an O-glycosylation site prediction tool was developed.
33664400	5	21	gly	non-glycosylated	612:627	arg1	non-glycosylated CD44	non-glycosylated CD44				OGER		CD44	P16070		We find that non-glycosylated CD44 favors the canonical sub-micromolar binding site, while glycosylated CD44 binds hyaluronan with an entirely different micromolar binding site.
33664400	5	29	gly	glycosylated	690:701	arg1	glycosylated CD44	glycosylated CD44				OGER		CD44	P16070		We find that non-glycosylated CD44 favors the canonical sub-micromolar binding site, while glycosylated CD44 binds hyaluronan with an entirely different micromolar binding site.
34869209	14	27	gly	occupied	2011:2018	arg2	The O-glycosites			The O-glycosites						O-glycosites	The O-glycosites shared between the S1 and RBD constructs, sites T323 and T523, were occupied by a similar range of Core 1 and 2 type O-glycans.
34869209	14	85	gly	sites	1985:1989	arg1	sites T323 and T523			sites T323 and T523						sites	The O-glycosites shared between the S1 and RBD constructs, sites T323 and T523, were occupied by a similar range of Core 1 and 2 type O-glycans.
34869209	14	122	gly	O-glycosites	1930:1941	arg2	The O-glycosites			The O-glycosites						O-glycosites	The O-glycosites shared between the S1 and RBD constructs, sites T323 and T523, were occupied by a similar range of Core 1 and 2 type O-glycans.
29755357	3	29	gly	N-glycosylation	474:488	arg2	one N-glycosylation site			one N-glycosylation site						site	Human LCN2 contains one N-glycosylation site conserved in other species.
30221662	10	23	gly	membrane	2380:2387	arg1	the O‑GlcNAc‑modified substrate protein SNAP29	vesicle‑associated membrane protein 8			the O‑GlcNAc‑modified substrate protein SNAP29	PUBTATOR		vesicle‑associated membrane protein 8	83730		Finally, co‑immunoprecipitation was used to determine the role of the O‑GlcNAc‑modified substrate protein SNAP29, which acted as an SNAP29‑syntaxin‑17 (STX17)‑vesicle‑associated membrane protein 8 (VAMP8) complex during disease progression.
30221662	10	30	gly	VAMP8	2400:2404	arg1	the O‑GlcNAc‑modified substrate protein SNAP29	8 (VAMP8			the O‑GlcNAc‑modified substrate protein SNAP29	PUBTATOR		8 (VAMP8	83730		Finally, co‑immunoprecipitation was used to determine the role of the O‑GlcNAc‑modified substrate protein SNAP29, which acted as an SNAP29‑syntaxin‑17 (STX17)‑vesicle‑associated membrane protein 8 (VAMP8) complex during disease progression.
30221662	10	86	gly	protein	2389:2395	arg1	the O‑GlcNAc‑modified substrate protein SNAP29	vesicle‑associated membrane protein 8			the O‑GlcNAc‑modified substrate protein SNAP29	PUBTATOR		vesicle‑associated membrane protein 8	83730		Finally, co‑immunoprecipitation was used to determine the role of the O‑GlcNAc‑modified substrate protein SNAP29, which acted as an SNAP29‑syntaxin‑17 (STX17)‑vesicle‑associated membrane protein 8 (VAMP8) complex during disease progression.
30221662	10	91	gly	‑vesicle‑associated	2360:2378	arg1	the O‑GlcNAc‑modified substrate protein SNAP29	vesicle‑associated membrane protein 8			the O‑GlcNAc‑modified substrate protein SNAP29	PUBTATOR		vesicle‑associated membrane protein 8	83730		Finally, co‑immunoprecipitation was used to determine the role of the O‑GlcNAc‑modified substrate protein SNAP29, which acted as an SNAP29‑syntaxin‑17 (STX17)‑vesicle‑associated membrane protein 8 (VAMP8) complex during disease progression.
33167210	4	67	gly	glycopeptide	684:695	arg2	a novel, high-throughput PSA glycopeptide workflow			a novel, high-throughput PSA glycopeptide workflow						glycopeptide	In this study, we developed a novel, high-throughput PSA glycopeptide workflow, based on matrix-assisted laser desorption/ionization-mass spectrometry, allowing the discrimination of sialic acid linkage isomers via the derivatization of glycopeptides.
33167210	4	76	gly	glycopeptides	864:876	arg2	glycopeptides			glycopeptides						glycopeptides	In this study, we developed a novel, high-throughput PSA glycopeptide workflow, based on matrix-assisted laser desorption/ionization-mass spectrometry, allowing the discrimination of sialic acid linkage isomers via the derivatization of glycopeptides.
35140700	2	52	gly	glycosylation	253:265	arg1	IgG	IgG				Cterm		IgG			The glycosylation patterns of IgG play a critical role in regulating the biological function and stability of IgG involved in the pathophysiology of many AIDs.
33516683	2	98	gly	glycoprotein	371:382	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Changes in glycoprotein and proteoglycan distribution are a hallmark of AS, yet valvular carbohydrate content remains virtually uncharacterized at the molecular level.
31181726	1	9	gly	P-glycoprotein	238:251	arg1	P-glycoprotein	P-glycoprotein				PUBTATOR		P-glycoprotein	5243		A series of novel 7,9-O-linked macrocyclic taxoids together with modification at the C2 position were synthesized, and their cytotoxicities against drug-sensitive and P-glycoprotein and βIII-tubulin overexpressed drug-resistant cancer cell lines were evaluated.
31879931	8	30	gly	N-glycopeptides	1456:1470	arg2	other biologically important N-glycopeptides			other biologically important N-glycopeptides						N-glycopeptides	The described procedures should be generally applicable for the synthesis of other biologically important N-glycopeptides.
28364041	0	54	gly	glycoprotein	43:54	arg1	the cell-surface glycoprotein CD146	the cell-surface glycoprotein CD146				Fterm		glycoprotein			Galectin-3 interacts with the cell-surface glycoprotein CD146 (MCAM, MUC18) and induces secretion of metastasis-promoting cytokines from vascular endothelial cells.
34200965	5	38	gly	glycosites	777:786	arg2	the respective glycosites			the respective glycosites						glycosites	Here we describe a new strategy to compare membrane glycoproteomes, thereby identifying proteins with altered glycan structures and the respective glycosites.
34089345	8	28	gly	glycoprotein	1266:1277	arg1	the MR glycoprotein ligands	the MR glycoprotein ligands				Fterm		glycoprotein			As lung cancer tissue and the lung cancer cell line A549 showed intense MR-Fc binding, we further investigated the MR glycoprotein ligands in those cells by immunoprecipitation and glycoproteomic analysis.
29920571	4	27	gly	glycopeptides	857:869	arg2	glycopeptides			glycopeptides						glycopeptides	Endowed with the high hydrophilicity of glutathione, the large surface area of the MOF and strong magnetic responsiveness of magnetic nanoparticles, the as-prepared mMOF@Au@GSH exhibited high selectivity (1 : 100) and great sensitivity (0.5 fmol μL-1) towards glycopeptides.
33270232	3	45	gly	carbohydrates	359:371	arg1	EhUbiquitin	EhUbiquitin			carbohydrates	Fterm		EhUbiquitin			Here we examined the immunogenicity and antigenicity of carbohydrates in EhUbiquitin.
31371405	4	52	gly	N-glycosylated	844:857	arg2	the Asn-141 residue	DDR-2		Asn-141 residue		PUBTATOR		DDR-2	180622	Asn-141 residue	Furthermore, we found that DDR-2 is N-glycosylated at the Asn-141 residue located in its discoidin domain, and mutation of this residue caused an axon regeneration defect.
30677534	6	51	gly	glycosylation	1268:1280	arg2	asparagine (N)-linked glycosylation sites			asparagine (N)-linked glycosylation sites						sites	Computational analysis showed that these mutations may lead to remarkable changes in the conformational structure and asparagine (N)-linked glycosylation sites of S1-NTD, which may be associated with the altered pathogenicity of these variant PEDV strains.
32825463	4	43	gly	fucosylated	500:510	arg1	fucosylated glycans				fucosylated glycans						However, little is known about how efficiently each unnatural Fuc analog is utilized by enzymes in the biosynthetic pathway of fucosylated glycans.
31826991	14	56	gly	have	2317:2320	arg1	H3 HAs AND one or more key high-mannose glycosites	H3 HAs			one or more key high-mannose glycosites	OGER		H3 HAs	Q92839		While it is known that both H1 and H3 HAs have one or more key high-mannose glycosites in the head region, little is known about similar glycosylation of LPAIV strains H2N1, H5N1, H6N1, or H11N9, which may pose future health risks.
28778927	2	16	gly	glycoproteins	270:282	arg1	three salivary glycoproteins	three salivary glycoproteins				Fterm		glycoproteins			In particular, the tick Rhipicephalus sanguineus has been shown to produce three salivary glycoproteins named "evasins," which bind to host chemokines, thereby inhibiting the recruitment of leukocytes to the location of the tick bite.
33073996	6	58	gly	site	938:941	arg1	glycans	PD-L1		N219 site	glycans			PD-L1	29126	N219 site	Molecular modeling of PD-L1/PD-1 interaction with N-glycans suggests that glycans at the N219 site of PD-L1 and N74 and N116 of PD-1 may be involved in glycan-glycan interactions, but the impact of this potential interaction on the protein function remains at this point unknown.
30593635	3	37	gly	ectodomain	573:582	arg1	11 N-linked glycans			ectodomain	11 N-linked glycans					ectodomain	Here, we describe an expression system for HCV E2 ectodomain (eE2) with 11 N-linked glycans and eight disulfide bonds, which combines lentivirus transduction of mammalian cells and a continuous growth, adherent cell bioreactor.
34324829	4	39	gly	N-glycosylated	796:809	arg1	N-glycosylated proteins	N-glycosylated proteins				Fterm		proteins			Chemoenzymatic synthesis using engineered endo-β-N-acetylglucosaminidases (ENGases) has been used extensively to modulate the chemistry of N-glycosylated proteins.
33263330	1	55	gly	glycoproteins	151:163	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Structural studies of glycoproteins and their complexes provide critical insights into their roles in normal physiology and disease.
34571967	6	10	gly	fucosylated	948:958	arg1	fucosylated glycans				fucosylated glycans						Glycans of untreated cells were compared to glycans of cells treated with inhibitors, including kifunensine, which inhibits the formation of complex- and hybrid-type structures, 2,4,7,8,9-Penta-O-acetyl-N-acetyl-3-fluoro-b-d-neuraminic acid methyl ester for sialylated glycans, 2-deoxy-2-fluorofucose, and 6-alkynyl fucose for fucosylated glycans.
34571967	6	36	gly	sialylated	879:888	arg1	sialylated glycans				sialylated glycans						Glycans of untreated cells were compared to glycans of cells treated with inhibitors, including kifunensine, which inhibits the formation of complex- and hybrid-type structures, 2,4,7,8,9-Penta-O-acetyl-N-acetyl-3-fluoro-b-d-neuraminic acid methyl ester for sialylated glycans, 2-deoxy-2-fluorofucose, and 6-alkynyl fucose for fucosylated glycans.
32764711	6	72	gly	asparagine-linked	1072:1088	arg1	hybrid glycans			asparagine	hybrid glycans					asparagine	High-mannose glycan with five mannose residues (Man5GlcNAc2), a complex biantennary galactosylated structure with core fucose (Gal2GlcNAc2Man3GlcNAc2Fuc), and a group of hybrid glycans with the composition Gal0-1GlcNAc1Man3-5GlcNAc2Fuc0-1 were confirmed as the main asparagine-linked oligosaccharides on the surface of TBEV derived from human neuronal cells.
32764711	6	72	gly	asparagine-linked	1072:1088	arg1	the main asparagine-linked oligosaccharides			asparagine	the main asparagine-linked oligosaccharides					asparagine	High-mannose glycan with five mannose residues (Man5GlcNAc2), a complex biantennary galactosylated structure with core fucose (Gal2GlcNAc2Man3GlcNAc2Fuc), and a group of hybrid glycans with the composition Gal0-1GlcNAc1Man3-5GlcNAc2Fuc0-1 were confirmed as the main asparagine-linked oligosaccharides on the surface of TBEV derived from human neuronal cells.
32764711	6	72	gly	asparagine-linked	1072:1088	arg1	High-mannose glycan			asparagine	High-mannose glycan					asparagine	High-mannose glycan with five mannose residues (Man5GlcNAc2), a complex biantennary galactosylated structure with core fucose (Gal2GlcNAc2Man3GlcNAc2Fuc), and a group of hybrid glycans with the composition Gal0-1GlcNAc1Man3-5GlcNAc2Fuc0-1 were confirmed as the main asparagine-linked oligosaccharides on the surface of TBEV derived from human neuronal cells.
32764711	6	72	gly	asparagine-linked	1072:1088	arg1	a complex biantennary galactosylated structure			asparagine	a complex biantennary galactosylated structure					asparagine	High-mannose glycan with five mannose residues (Man5GlcNAc2), a complex biantennary galactosylated structure with core fucose (Gal2GlcNAc2Man3GlcNAc2Fuc), and a group of hybrid glycans with the composition Gal0-1GlcNAc1Man3-5GlcNAc2Fuc0-1 were confirmed as the main asparagine-linked oligosaccharides on the surface of TBEV derived from human neuronal cells.
32986846	5	3	gly	glycoproteins	837:849	arg1	brain glycoproteins	brain glycoproteins				Fterm		glycoproteins			Glycans were enzymatically liberated from brain glycoproteins and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
32986846	5	50	gly	liberated	816:824	arg2	brain glycoproteins AND Glycans	brain glycoproteins			Glycans	Fterm		glycoproteins			Glycans were enzymatically liberated from brain glycoproteins and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
31019496	4	64	gly	proteins	745:752	arg1	polysaccharides	proteins			polysaccharides	Fterm		proteins			We extracted and determined the amount of polysaccharides and proteins from representative bacterial biofilms.
32553552	3	60	gly	glycoproteins	437:449	arg1	membrane-bound midgut glycoproteins	membrane-bound midgut glycoproteins				Fterm		glycoproteins			However, there is still little information about the N-glycome of membrane-bound midgut glycoproteins in Lepidoptera and other insect groups.
30988294	0	27	gly	flippase	53:60	arg1	the Lipid II flippase MurJ	flippase			the Lipid II flippase MurJ	Fterm		flippase			Visualizing conformation transitions of the Lipid II flippase MurJ.
31852790	9	25	gly	glycosylation	1702:1714	arg2	residue 144			residue 144						residue 144	Mice were vaccinated with VLPs expressing HA antigens that lacked a glycosylation site at residue 144 and a deleted lysine at position 147 residue were more effective at protecting against morbidity and mortality following infection with pandemic-like and seasonal-like H1N1 influenza viruses.IMPORTANCE There is a great need to develop broadly reactive or universal vaccines against influenza viruses.
31852790	9	25	gly	glycosylation	1702:1714	arg2	a glycosylation site			a glycosylation site						site	Mice were vaccinated with VLPs expressing HA antigens that lacked a glycosylation site at residue 144 and a deleted lysine at position 147 residue were more effective at protecting against morbidity and mortality following infection with pandemic-like and seasonal-like H1N1 influenza viruses.IMPORTANCE There is a great need to develop broadly reactive or universal vaccines against influenza viruses.
31616924	3	67	gly	O-glycosylation	536:550	arg1	APOE	APOE				PUBTATOR		APOE	348		To better understand the O-glycosylation on this critical molecule and differences across tissues, we analyzed the O-glycosylation on APOE isolated from the plasma and CSF of aged individuals.
32022078	0	60	gly	structures	27:36	arg1	human urinary glycoproteins	glycoproteins			structures	Fterm		glycoproteins			Novel O-linked sialoglycan structures in human urinary glycoproteins.
32022078	0	61	gly	glycoproteins	55:67	arg1	human urinary glycoproteins	human urinary glycoproteins				Fterm		glycoproteins			Novel O-linked sialoglycan structures in human urinary glycoproteins.
30850477	1	30	gly	glycans	137:143	arg1	their V regions			their V regions	their V regions		Site			regions	Abs can acquire N-linked glycans in their V regions during Ag-specific B cell responses.
31479573	4	57	gly	glycosylated	919:930	arg1	the wild-type glycosylated A1 domain			the wild-type glycosylated A1 domain						domain	OBJECTIVES The platelet adhesive function, thermodynamic stability, and conformational dynamics of the wild-type and type 2M G1324S A1 domain lacking glycosylation (Escherichia coli) are compared with the wild-type glycosylated A1 domain (HEK293 cell culture) to decipher the self-inhibitory mechanism.
33030205	6	49	gly	residues	1332:1339	arg1	the bowl to make classic aromatic ring-carbohydrate CH-π interactions			tyrosine residues	the bowl to make classic aromatic ring-carbohydrate CH-π interactions					tyrosine residues	The proline is bound in the center of a bowl formed by four functionally conserved aromatic amino acid side chains while the glycan wraps around one of the tyrosine residues in the bowl to make classic aromatic ring-carbohydrate CH-π interactions.
33554253	5	13	gly	have	802:805	arg1	Nonrecombinant Rb-IgGs AND O-glycans	Nonrecombinant Rb-IgGs			O-glycans	Cterm		IgGs	16059		Nonrecombinant Rb-IgGs have N- and O-glycans, and the presence of O-glycans close to the hinge region of the rRb-IgGs might affect the susceptibility of these antibodies to SrtA cleavage.
33554253	5	13	gly	have	802:805	arg1	Nonrecombinant Rb-IgGs AND N-	Nonrecombinant Rb-IgGs			N-	Cterm		IgGs	16059		Nonrecombinant Rb-IgGs have N- and O-glycans, and the presence of O-glycans close to the hinge region of the rRb-IgGs might affect the susceptibility of these antibodies to SrtA cleavage.
34864058	0	90	gly	fucosylation	53:64	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cells.
34864058	0	69	gly	glycoproteins	69:81	arg1	glycoproteins	glycoproteins			fucosylation	Fterm		glycoproteins			Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cells.
28302723	1	11	gly	proteins	258:265	arg1	O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			O-GlcNAc is a regulatory post-translational modification of nucleocytoplasmic proteins that has been implicated in multiple biological processes, including transcription.
30535277	3	44	gly	fucosylated	429:439	arg1	fucosylated N/O-glycans				fucosylated N/O-glycans						Here, we further investigated the alterations of fucosylated N/O-glycans recognized by LTL in saliva from 120 subjects (30 healthy volunteers (HV), 30 patients with hepatitis B (HB), 30 patients with hepatic cirrhosis (HC), and 30 patients with hepatocellular carcinoma (HCC)) using salivary microarrys and MALDI-TOF/TOF-MS.
32699088	7	4	gly	domain	1260:1265	arg1	the head domain glycans				the head domain glycans						It also revealed that N1 stability is more affected by the head domain glycans, suggesting N2 is more amenable to glycan additions.
32699088	7	67	gly	head	1255:1258	arg1	the head domain glycans				the head domain glycans						It also revealed that N1 stability is more affected by the head domain glycans, suggesting N2 is more amenable to glycan additions.
31837192	2	24	gly	glycoproteins	473:485	arg1	plant-derived glycoproteins	plant-derived glycoproteins				Fterm		glycoproteins			Plants, particularly Nicotiana benthamiana, are attractive hosts to produce recombinant glycoproteins, and recent advances in glyco-engineering facilitate customized N-glycosylation of plant-derived glycoproteins.
31837192	2	36	gly	glycoproteins	362:374	arg1	recombinant glycoproteins	recombinant glycoproteins				Fterm		glycoproteins			Plants, particularly Nicotiana benthamiana, are attractive hosts to produce recombinant glycoproteins, and recent advances in glyco-engineering facilitate customized N-glycosylation of plant-derived glycoproteins.
31837192	2	78	gly	N-glycosylation	440:454	arg1	plant-derived glycoproteins	plant-derived glycoproteins				Fterm		glycoproteins			Plants, particularly Nicotiana benthamiana, are attractive hosts to produce recombinant glycoproteins, and recent advances in glyco-engineering facilitate customized N-glycosylation of plant-derived glycoproteins.
33139572	8	34	gly	heterogeneity	1392:1404	arg1	glycans				glycans						Finally, based on the vast heterogeneity of glycans, we constructed a core glyco-synthesis map to delineate the glycosylation pathway responsible for the glycan diversity during the PFC development.
31879931	6	17	gly	glycosylation	931:943	arg2	a predetermined glycosylation site			a predetermined glycosylation site						site	The first step is to prepare the GlcNAc peptide through automated solid-phase peptide synthesis (SPPS) by incorporating an Asn-linked GlcNAc moiety at a predetermined glycosylation site; and the second step is to transfer an N-glycan from the corresponding N-glycan oxazoline en bloc to the GlcNAc peptide by an endoglycosidase or its efficient glycosynthase mutant.
31879931	6	48	gly	Asn-linked	887:896	arg1	an Asn-linked GlcNAc moiety			Asn	an Asn-linked GlcNAc moiety					Asn	The first step is to prepare the GlcNAc peptide through automated solid-phase peptide synthesis (SPPS) by incorporating an Asn-linked GlcNAc moiety at a predetermined glycosylation site; and the second step is to transfer an N-glycan from the corresponding N-glycan oxazoline en bloc to the GlcNAc peptide by an endoglycosidase or its efficient glycosynthase mutant.
30169771	2	20	gly	repeats	317:323	arg1	the Notch receptors	receptors			repeats	Fterm		receptors			One of the O-linked glycans added to epidermal growth factor-like (EGF) repeats in animal proteins including the Notch receptors is the xylose-xylose-glucose-O oligosaccharide.
30169771	2	20	gly	repeats	317:323	arg1	animal proteins	proteins			repeats	Fterm		proteins			One of the O-linked glycans added to epidermal growth factor-like (EGF) repeats in animal proteins including the Notch receptors is the xylose-xylose-glucose-O oligosaccharide.
31341641	1	32	gly	glycoprotein	238:249	arg1	the surface envelope glycoprotein (gp120)	the surface envelope glycoprotein (gp120)				Fterm		glycoprotein			The transmission fitness and pathogenesis of HIV-1 is disproportionately influenced by evolution in the five variable regions (V1-V5) of the surface envelope glycoprotein (gp120).
34726367	4	31	gly	PglI	666:669	arg1	GlcTF	PglI			GlcTF	Cterm		PglI			Although all 4 strains encode the PglI glucosyltransferase (GlcTF), one aspartate in the DXDD motif was missing, an alteration also present in ∼4% of all available PglI sequences.
32535395	10	16	gly	fucosylated	1495:1505	arg1	fewer fucosylated termini			fewer fucosylated termini						termini	Glycosylation changed dramatically in pregnancy in the luminal and superficial gland epithelium, with fewer fucosylated termini, more N-acetyl galactosamine residues, together with an overall reduction in sialic acid and several other sugar structures.
31604106	8	14	gly	O-glycosylation	1517:1531	arg1	intracellular proteins	intracellular proteins				Fterm		proteins			The LC-MS/MS analysis and Phaseolus vulgaris leucoagglutinin (PHA-L) lectin blot analysis showed that Ugp2 overexpression significantly increased the total galactosylation levels of intracellular proteins and the O-glycosylation of recombinant hCG protein.
31604106	8	14	gly	O-glycosylation	1517:1531	arg1	recombinant hCG protein	recombinant hCG protein				PUBTATOR		hCG protein	93659		The LC-MS/MS analysis and Phaseolus vulgaris leucoagglutinin (PHA-L) lectin blot analysis showed that Ugp2 overexpression significantly increased the total galactosylation levels of intracellular proteins and the O-glycosylation of recombinant hCG protein.
30697857	8	55	gly	N-glycosylated	1179:1192	arg1	N-glycosylated proteins	N-glycosylated proteins				Fterm		proteins			Strikingly, we observe a positive E-R correlation among N-glycosylated proteins.
33092920	0	55	gly	glycoproteins	28:40	arg1	the goat milk glycoproteins	the goat milk glycoproteins				Fterm		glycoproteins			Alteration of the goat milk glycoproteins N/O-glycome at different lactation stages.
31194940	4	34	gly	N88	730:732	arg1	a conserved N-linked glycan			N88	a conserved N-linked glycan			Peptide		N88	We show that ACS202 CDRH3 forms a "β strand" interaction with the exposed hydrophobic FP and recognizes a continuous region of gp120, including a conserved N-linked glycan at N88.
32080177	4	31	gly	FUT8	550:553	arg1	a biantennary complex N-glycan	structure of FUT8			a biantennary complex N-glycan	PUBTATOR		structure of FUT8	2530		Here, we report the crystal structure of FUT8 complexed with GDP and a biantennary complex N-glycan (G0), which provides insight into both substrate recognition and catalysis.
29672582	4	90	gly	glycoform	918:926	arg1	each IgG4 glycoform	each IgG4 glycoform				OGER		IgG4	P01861		We also compared the concentration of each IgG4 glycoform between patients with and without hypocomplementemia and individual organ involvement (kidney, pancreas, lymph node) in IgG4RD.
31892091	2	64	gly	used	428:431	arg2	The O126-glycopeptide	rhEPO		The O126-glycopeptide				rhEPO	P29676	O126-glycopeptide	The O126-glycopeptide of rhEPO was used to optimize the methodology given its importance in quality control of biopharmaceuticals and doping analysis.
31892091	2	40	gly	O126-glycopeptide	397:413	arg2	The O126-glycopeptide	rhEPO		O126-glycopeptide		OGER		rhEPO	P29676	O126-glycopeptide	The O126-glycopeptide of rhEPO was used to optimize the methodology given its importance in quality control of biopharmaceuticals and doping analysis.
31337705	7	16	gly	sialylated	1086:1095	arg1	no sialylated Lewis A				no sialylated Lewis A						MS and immunohistochemistry analyses indicated that suLeA motifs were present predominantly on O-linked glycans initiated by GalNAc residues, but no sialylated Lewis A was detected.
31516400	1	13	gly	glycoprotein	146:157	arg1	BACKGROUND N-linked glycoprotein	BACKGROUND N-linked glycoprotein				Fterm		glycoprotein			BACKGROUND N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research.
33677548	1	48	gly	glycosylation	304:316	arg2	glycosylation sites			glycosylation sites						sites	Recent years have seen great advances in the development of glycoproteomics protocols and methods resulting in a sustainable increase in the reporting proteins, their attached glycans and glycosylation sites.
30838579	2	11	gly	glycoprotein	243:254	arg1	N-linked glycoprotein	N-linked glycoprotein				Fterm		glycoprotein			One category of NCPs is termed the SIBLING (small integrin-binding ligand, N-linked glycoprotein) family, which includes osteopontin (OPN), bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP), etc.
34888356	9	22	gly	terminus	1907:1914	arg1	α2,3-sialic acid			terminus	α2,3-sialic acid					terminus	In particular, it was confirmed that α2,3-sialic acid at the terminus of biantennary N-glycan was the epitope associated with BD.
30623643	4	56	gly	glycoproteins	621:633	arg1	Mucin glycoproteins	Mucin glycoproteins				PUBTATOR		Mucin glycoproteins	100508689		Mucin glycoproteins, for example, comprise an abundant source of carbon in the gut, oral cavity, respiratory tract, and other mucosal surfaces but are not commercially available.
33435207	1	52	gly	protein	233:239	arg1	the four N-linked glycans	protein			the four N-linked glycans	Fterm		protein			The structures of the four N-linked glycans from the prototype chlorovirus PBCV-1 major capsid protein do not resemble any other glycans in the three domains of life.
31783892	0	28	gly	glycosylation	10:22	arg1	PD-1	PD-1				OGER		PD-1	Q15116		Targeting glycosylation of PD-1 to enhance CAR-T cell cytotoxicity.
33340519	2	46	gly	has	301:303	arg1	SARS-CoV-2 AND the capacity to bind sialic acid which is a common, and highly variable, terminal modification of glycans	SARS-CoV-2			the capacity to bind sialic acid which is a common, and highly variable, terminal modification of glycans	OGER		SARS	P49591		One potential contribution are differences in the glycosylation of target human cells, particularly as SARS-CoV-2 has the capacity to bind sialic acid which is a common, and highly variable, terminal modification of glycans.
34813786	7	50	gly	glycoproteins	1283:1295	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			These results could imply that an ancestral tunicate ST3Gal-I in C. savignyi would prefer O-glycan onto glycoproteins as its sialic acid acceptor than vertebrate enzymes.
29969454	3	63	gly	glycosylation	606:618	arg2	two potential N-linked glycosylation sites			two potential N-linked glycosylation sites						sites	Our findings showed that 16 residues that changed antigenicity, two potential N-linked glycosylation sites, and one amino acid in the receptor binding site of the HA protein changed significantly from 2011-2014.
32409993	5	31	gly	occupied	716:723	arg2	all 14 potential N-glycosylation sites			all 14 potential N-glycosylation sites						sites	We found that all 14 potential N-glycosylation sites on BRI1 were occupied with oligosaccharides.
32409993	5	62	gly	N-glycosylation	681:695	arg2	all 14 potential N-glycosylation sites			all 14 potential N-glycosylation sites						sites	We found that all 14 potential N-glycosylation sites on BRI1 were occupied with oligosaccharides.
29793953	6	5	gly	glycosylated	1173:1184	arg1	Tagged NTCP	Tagged NTCP				PUBTATOR		Tagged NTCP	6554		Tagged NTCP introduced to both HepG2 and HepaRG cells was glycosylated, with N5 and N11 being sites of N-linked glycosylation.
29793953	6	34	gly	glycosylation	1227:1239	arg2	sites			sites						sites	Tagged NTCP introduced to both HepG2 and HepaRG cells was glycosylated, with N5 and N11 being sites of N-linked glycosylation.
34379416	7	64	gly	unglycosylated	1266:1279	arg1	hPDI	hPDI				OGER		hPDI	P07237		In addition, we compare our results on yPDI with structural information of homologous proteins such as human PDI (hPDI), which is natively unglycosylated.
34379416	7	64	gly	unglycosylated	1266:1279	arg1	human PDI	human PDI				PUBTATOR		PDI	64714		In addition, we compare our results on yPDI with structural information of homologous proteins such as human PDI (hPDI), which is natively unglycosylated.
33177111	0	33	gly	CD55	15:18	arg1	Sialylation	CD55			Sialylation	OGER		CD55	P08174		Sialylation of CD55 by ST3GAL1 Facilitates Immune Evasion in Cancer.
33026663	9	46	gly	glycoproteins	1329:1341	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N-glycans on the surface charge and enzyme stability in regard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.
33026663	9	74	gly	attached	1317:1324	arg2	glycoproteins AND glycan structures	glycoproteins			glycan structures	Fterm		glycoproteins			These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N-glycans on the surface charge and enzyme stability in regard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.
31800099	2	29	gly	glycosylation	386:398	arg1	B4GALT1	B4GALT1				PUBTATOR		B4GALT1	2683		We set out to further investigate this by studying the effects of defective glycosylation on plasma lipids in patients with B4GALT1-CDG, caused by a mutation in B4GALT1 with defective N-linked glycosylation.
34379416	5	49	gly	glycosylated	824:835	arg1	glycosylated and unglycosylated yPDI	glycosylated and unglycosylated yPDI				Cterm		yPDI	64714		We compare simulations of glycosylated and unglycosylated yPDI and find that the presence of glycan-glycan and glycan-protein interactions influences the flexibility of PDI in different ways.
34379416	5	52	gly	unglycosylated	841:854	arg1	glycosylated and unglycosylated yPDI	glycosylated and unglycosylated yPDI				Cterm		yPDI	64714		We compare simulations of glycosylated and unglycosylated yPDI and find that the presence of glycan-glycan and glycan-protein interactions influences the flexibility of PDI in different ways.
34869209	10	10	gly	N-glycosites	1244:1255	arg2	N-glycosites			N-glycosites						N-glycosites	Comparison of the whole cell-derived WA1 and D614G spike proteins revealed that N-glycosites local to the mutation site appeared to be more readily detected, hinting that these sites are more exposed to glycosylation machinery.
32627435	6	65	gly	fucosylated	1246:1256	arg1	fucosylated structures				fucosylated structures						Specifically, terminal galactose on afucosylated IgG1 mAbs enhanced ADCC activity with impact coefficients (ADCC%/Gal%) more than 20, but had minimal influence on ADCC activity on fucosylated structures with impact coefficient in the range of 0.1-0.2.
34917767	8	65	gly	ST3GAL5	1257:1263	arg1	GM3	ST3GAL5			GM3	PUBTATOR		ST3GAL5	8869		Quantitative plasma GSL profiles discriminated among ST3GAL5 genotypes: GM3 and GD3 were undetectable in ST3GAL5 c.694C > T homozygotes, who had markedly elevated lactosylceramide (19.17 ± 4.20 nmol/ml) relative to heterozygous siblings (9.62 ± 2.46 nmol/ml) and wild type controls (6.55 ± 2.16 nmol/ml).
34917767	8	65	gly	ST3GAL5	1257:1263	arg1	GD3	ST3GAL5			GD3	PUBTATOR		ST3GAL5	8869		Quantitative plasma GSL profiles discriminated among ST3GAL5 genotypes: GM3 and GD3 were undetectable in ST3GAL5 c.694C > T homozygotes, who had markedly elevated lactosylceramide (19.17 ± 4.20 nmol/ml) relative to heterozygous siblings (9.62 ± 2.46 nmol/ml) and wild type controls (6.55 ± 2.16 nmol/ml).
34520098	1	24	gly	sialylated	125:134	arg1	a fully sialylated tetraantennary N-glycan				a fully sialylated tetraantennary N-glycan						The chemical synthesis of a fully sialylated tetraantennary N-glycan has been achieved for the first time by using the diacetyl strategy, in which NHAc is protected as NAc2 to improve reactivity by preventing intermolecular hydrogen bonds.
29093093	2	56	gly	glycoprotein	288:299	arg1	the spike (S) glycoprotein	the spike (S) glycoprotein				Fterm		glycoprotein			They utilize the spike (S) glycoprotein anchored in the viral envelope to mediate host attachment and fusion of the viral and cellular membranes to initiate infection.
32965048	9	71	gly	N-glycosites	1849:1860	arg2	200 N-glycosites			200 N-glycosites						N-glycosites	Next, 146 linkage structures and 200 N-glycosites were confirmed with structure-diagnostic and GlcNAc-containing site-determining product ions, respectively.
33004438	1	65	gly	Asn-linked	147:156	arg1	Mammalian Asn-linked glycans			Asn	Mammalian Asn-linked glycans					Asn	Mammalian Asn-linked glycans are extensively processed as they transit the secretory pathway to generate diverse glycans on cell surface and secreted glycoproteins.
33004438	1	81	gly	glycoproteins	287:299	arg1	secreted glycoproteins	secreted glycoproteins				Fterm		glycoproteins			Mammalian Asn-linked glycans are extensively processed as they transit the secretory pathway to generate diverse glycans on cell surface and secreted glycoproteins.
31696430	3	50	gly	glycosylation	659:671	arg2	putative N-linked glycosylation sites			putative N-linked glycosylation sites						sites	The structural profile of 5-HTHdh displayed key features of G protein-coupled receptors, including seven hydrophobic transmembrane domains, putative N-linked glycosylation sites, and several phosphorylation consensus motifs.
33270232	8	33	gly	glycoprotein	957:968	arg1	anti-Ub glycoprotein antibodies	anti-Ub glycoprotein antibodies				Fterm		glycoprotein			Conversely, anti-Ub glycoprotein antibodies are well maintained in circulation.
31176190	4	6	gly	glycoproteins	612:624	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This is not often the case with glycopeptides or glycoproteins as these may reveal increased complexity in terms of heterogeneity in protein-glycan stoichiometry and restricted enzyme accessibility.
31176190	4	36	gly	glycopeptides	595:607	arg2	glycopeptides			glycopeptides						glycopeptides	This is not often the case with glycopeptides or glycoproteins as these may reveal increased complexity in terms of heterogeneity in protein-glycan stoichiometry and restricted enzyme accessibility.
30242111	5	53	gly	glycosites	903:912	arg2	1733 N-linked glycosites			1733 N-linked glycosites						glycosites	A total of 2946 proteins, 1733 N-linked glycosites and 4100 phosphosites were identified and quantified in this study, disclosing overrepresented processes related to oxidative stress, protein folding and regulation of apoptosis in past-infected placentas Moreover, AKT and ERK signaling pathways activation, together with clinical data, were further correlated to an increased apoptosis in past-infected placentas.
30587575	1	3	gly	serine	181:186	arg1	O-GlcNAcylation			serine	O-GlcNAcylation					serine	O-GlcNAcylation is a post-translational modification of a protein serine or threonine residue catalyzed by O-GlcNAc transferase (OGT) in the nucleus and cytoplasm.
30587575	1	20	gly	residue	201:207	arg1	O-GlcNAcylation			threonine residue	O-GlcNAcylation					threonine residue	O-GlcNAcylation is a post-translational modification of a protein serine or threonine residue catalyzed by O-GlcNAc transferase (OGT) in the nucleus and cytoplasm.
30135544	3	53	gly	glycosylation	400:412	arg1	human PrP	human PrP				PUBTATOR		PrP	5621		In this study, we attempt to elucidate the effects of glycosylation on the aggregation and toxicity of human PrP.
33197804	11	71	gly	profiles	1536:1543	arg1	the SMG	SMG			profiles	OGER		SMG	P62309		CONCLUSION There are three secretory mucins with different glycan profiles in the SMG of mice, and their expression patterns change according to the period of the aging process.
31511323	11	14	gly	glycosylation	1654:1666	arg1	NaV1.5	NaV1.5				PUBTATOR		NaV1	Q8NEY1		In conclusion, our results indicate that N-linked glycosylation of β2 is required for surface localization of NaV1.5, a property that is often defective in inherited cardiac arrhythmias.
31511323	11	14	gly	glycosylation	1654:1666	arg1	β2	β2				PUBTATOR		2	170589		In conclusion, our results indicate that N-linked glycosylation of β2 is required for surface localization of NaV1.5, a property that is often defective in inherited cardiac arrhythmias.
33377107	4	28	gly	glycoproteins	538:550	arg1	N- and/or O-linked glycoproteins	N- and/or O-linked glycoproteins				Fterm		glycoproteins			This procedure can be modified and applied to a variety of N- and/or O-linked glycoproteins.
34343291	6	55	gly	ectodomain	963:972	arg1	leucine-rich repeat L1 domain			ectodomain	leucine-rich repeat L1 domain					ectodomain	We studied the effects of removal of sialic acid on the glycan, as well as on the dynamics of leucine-rich repeat L1 domain of the IR ectodomain.
34343291	6	76	gly	leucine-rich	923:934	arg1	leucine-rich repeat L1 domain			leucine	leucine-rich repeat L1 domain					leucine	We studied the effects of removal of sialic acid on the glycan, as well as on the dynamics of leucine-rich repeat L1 domain of the IR ectodomain.
31281932	7	33	gly	glycoprotein	959:970	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			sialyltransferase using glycoprotein substrates in a 96-well microtiter-plate-based assay.
33167210	6	64	gly	glycopeptides	1009:1021	arg2	Forty-four glycopeptides			Forty-four glycopeptides						glycopeptides	Forty-four glycopeptides were quantified in all samples, showing mainly complex-type glycans with high levels of fucosylation and sialylation.
33030205	0	60	gly	glycopeptide	43:54	arg2	a proline-specific glycopeptide recognition domain			a proline-specific glycopeptide recognition domain						glycopeptide	Structural evidence for a proline-specific glycopeptide recognition domain in an O-glycopeptidase.
30566493	3	53	gly	glycosylation	804:816	arg2	four potential N-linked glycosylation sites			four potential N-linked glycosylation sites						sites	Isolated envelope sequences were analyzed by deep-sequencing showing a small number of dominant changes, including the loss of four potential N-linked glycosylation sites and disruption of the V1/V2 loop.
34279906	2	11	gly	glycosylation	566:578	arg2	glycosylation sites			glycosylation sites						sites	Native mass spectrometry has transformed our ability to study glycoproteins, and despite advances in high-resolution instrumentation, there are comparatively a few studies demonstrating its potential with data largely limited to an overall measure of monosaccharide composition for all glycans across glycosylation sites for a given protein.
34279906	2	24	gly	glycoproteins	327:339	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Native mass spectrometry has transformed our ability to study glycoproteins, and despite advances in high-resolution instrumentation, there are comparatively a few studies demonstrating its potential with data largely limited to an overall measure of monosaccharide composition for all glycans across glycosylation sites for a given protein.
31600726	3	18	gly	N-glycans	330:338	arg1	the FSHβ subunit	subunit			N-glycans	Fterm		subunit			Differences in the number of N-glycans on the FSHβ subunit result in distinct glycoforms: hypo-glycosylated (FSH21/18, glycans absent on either Asn24 or Asn7, respectively) or fully-glycosylated (FSH24, glycans present on both Asn7 and Asn24).
31600726	3	82	gly	present	512:518	arg1	Asn7 AND glycans			Asn7 and Asn24	glycans			FSH	14308	Asn7 and Asn24	Differences in the number of N-glycans on the FSHβ subunit result in distinct glycoforms: hypo-glycosylated (FSH21/18, glycans absent on either Asn24 or Asn7, respectively) or fully-glycosylated (FSH24, glycans present on both Asn7 and Asn24).
28734139	10	30	gly	glycosylation	1548:1560	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Both substitutions carried by this strain were located on antigenic sites and caused losses of N-linked glycosylation sites of the virus, which could potentially affect viral antigenicity.
29980609	5	14	gly	glycoproteins	951:963	arg1	AICL glycoproteins	AICL glycoproteins				PUBTATOR		AICL glycoproteins	9976		Cys87 residing within the C-type lectin-like domain not only ensures stable homodimerization of AICL glycoproteins by disulfide bonding, but Cys87 is also required for efficient cell surface expression of AICL homodimers and essential for AICL-NKp80 interaction.
31821735	6	50	gly	glycosylation	1163:1175	arg1	sites			sites						sites	We employ a well-established experimental system that uses N-linked glycosylation of sites within the protein as a measure to assess the extent of membrane insertion and the topology it assumes when inserted into the ER membrane.
35026558	4	19	gly	residues	855:862	arg1	the saccharide primers			residues in	the saccharide primers					residues in	The amounts of glycosylated products were dependent on the amino acid residues in the saccharide primers.
30980499	8	12	gly	N-linked	1009:1016	arg1	biglycan	N-linked			biglycan	Cterm		N-linked			Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified.
30980499	8	69	gly	glycoproteins	1018:1030	arg1	biglycan	transforming growth factor beta 1			biglycan	PUBTATOR		transforming growth factor beta 1	7040		Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified.
30980499	8	69	gly	glycoproteins	1018:1030	arg1	biglycan	COL1A1			biglycan	PUBTATOR		COL1A1	1277		Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified.
30980499	8	69	gly	glycoproteins	1018:1030	arg1	biglycan	glycoproteins			biglycan	Fterm		glycoproteins			Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified.
30980499	8	69	gly	glycoproteins	1018:1030	arg1	dentin sialoprotein	dentin sialoprotein			biglycan	PUBTATOR		dentin sialoprotein	1834		Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified.
32384234	4	51	gly	glycopeptides	962:974	arg2	glycopeptides			glycopeptides						glycopeptides	Consistent with previous reports by others, we found that sulfation and sialylation drastically alter the MS2 fragmentation pattern of glycopeptides in negative mode and the characteristic features identified can be utilized to program the most informative MS3 on the glycan moiety itself.
32364434	2	33	gly	glycoproteins	393:405	arg1	therapeutic glycoproteins	therapeutic glycoproteins				Fterm		glycoproteins			The stability, serum half-life, immunogenicity, and biological activity of therapeutic glycoproteins, including antibodies, vaccines, and biomarkers, are regulated by their glycosylation profile.
30076101	1	48	gly	glycoprotein	280:291	arg1	Env	Env				PUBTATOR		Env	100616444		An important class of HIV-1 broadly neutralizing antibodies, termed the VRC01 class, targets the conserved CD4-binding site (CD4bs) of the envelope glycoprotein (Env).
30076101	1	48	gly	glycoprotein	280:291	arg1	the envelope glycoprotein	the envelope glycoprotein				PUBTATOR		envelope glycoprotein	100616444		An important class of HIV-1 broadly neutralizing antibodies, termed the VRC01 class, targets the conserved CD4-binding site (CD4bs) of the envelope glycoprotein (Env).
34631661	1	53	gly	glycosylated	269:280	arg1	the extensively glycosylated spike (S) protein	the extensively glycosylated spike (S) protein				Fterm		protein			The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus utilizes the extensively glycosylated spike (S) protein protruding from the viral envelope to bind to angiotensin-converting enzyme-related carboxypeptidase (ACE2) as its primary receptor to mediate host-cell entry.
31067000	13	88	gly	glycosylation	1868:1880	arg1	the IgG ACPA V domain			the IgG ACPA V domain						domain	Based on these findings, we propose that glycosylation of the IgG ACPA V domain represents a predictive marker for RA development in ACPA-positive individuals and may serve to better target prevention measures.
32817340	4	42	gly	modifications	735:747	arg1	S-layer protein	protein			modifications	Fterm		protein			Here we describe the structural characterization of the N-linked glycan modifications on the archaellins and S-layer protein of Methanothermococcus thermolithotrophicus, a methanogen that grows optimally at 65 °C. SDS-PAGE and MS analysis revealed that the sheared archaella are composed principally of two of the four predicted archaellins, FlaB1 and FlaB3, which are modified with a branched, heptameric glycan at all N-linked sequons except for the site closest to the N termini of both proteins.
32817340	4	65	gly	sequons	1092:1098	arg1	a branched, heptameric glycan				a branched, heptameric glycan						Here we describe the structural characterization of the N-linked glycan modifications on the archaellins and S-layer protein of Methanothermococcus thermolithotrophicus, a methanogen that grows optimally at 65 °C. SDS-PAGE and MS analysis revealed that the sheared archaella are composed principally of two of the four predicted archaellins, FlaB1 and FlaB3, which are modified with a branched, heptameric glycan at all N-linked sequons except for the site closest to the N termini of both proteins.
31722217	7	3	gly	glycans	1129:1135	arg1	these sites			these sites	these sites		Site			sites	Our work shows the combinatorial and context-dependent roles of GlcNAc-fucose-O glycans on these sites in Drosophila Notch-ligand interactions.
29920571	5	17	gly	glycopeptides	939:951	arg2	glycopeptides			glycopeptides						glycopeptides	Furthermore, it also achieved outstanding performance in enriching glycopeptides from complex biological samples.
30023808	5	19	gly	glycoproteins	1243:1255	arg1	323 different glycoproteins	323 different glycoproteins				Fterm		glycoproteins			Moreover, Fe3O4-PEI-pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified.
30023808	5	38	gly	glycopeptides	1107:1119	arg2	glycopeptides			glycopeptides						glycopeptides	Moreover, Fe3O4-PEI-pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified.
30023808	5	74	gly	glycopeptides	1201:1213	arg2	449 N-linked glycopeptides			449 N-linked glycopeptides						glycopeptides	Moreover, Fe3O4-PEI-pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified.
30023808	5	86	gly	glycosylation	1265:1277	arg2	476 glycosylation sites			476 glycosylation sites						sites	Moreover, Fe3O4-PEI-pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified.
32784866	5	10	gly	glycans	803:809	arg1	individual chains	chains			glycans	OGER		chains	P17813		The distribution of glycans on individual chains was also affected, with the γ chain, responsible for physiological functions of fibrinogen (such as coagulation and platelet aggregation), being most prone to these alterations.
33525794	1	12	gly	glycoproteins	340:352	arg1	complex glycoproteins	complex glycoproteins				Fterm		glycoproteins			The demonstration more than a decade ago that glycoproteins could be produced in Escherichia coli cells equipped with the N-linked protein glycosylation machinery from Campylobacter jejuni opened the door to using simple bacteria for the expression and engineering of complex glycoproteins.
33525794	1	34	gly	glycoproteins	110:122	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The demonstration more than a decade ago that glycoproteins could be produced in Escherichia coli cells equipped with the N-linked protein glycosylation machinery from Campylobacter jejuni opened the door to using simple bacteria for the expression and engineering of complex glycoproteins.
31337704	7	95	gly	glycoproteins	1759:1771	arg1	specific glycoproteins	specific glycoproteins				Fterm		glycoproteins			We observed that MAGT1-dependent glycosylation is sensitive to Mg2+ levels and that reduced Mg2+ impairs immune-cell function via the loss of specific glycoproteins.
34450331	0	57	gly	glycoprotein	25:36	arg1	glycoprotein conformational changes	glycoprotein conformational changes				Fterm		glycoprotein			Systems-wide analysis of glycoprotein conformational changes by limited deglycosylation assay.
32168410	1	8	gly	glycoprotein	189:200	arg1	The protective/inhibitory B subunits	The protective/inhibitory B subunits				Fterm		subunits			BACKGROUND The protective/inhibitory B subunits of coagulation factor XIII (FXIII-B) is a ~80 kDa glycoprotein containing two N-glycosylation sites.
32168410	1	8	gly	glycoprotein	189:200	arg1	a ~80 kDa glycoprotein	a ~80 kDa glycoprotein				Fterm		glycoprotein			BACKGROUND The protective/inhibitory B subunits of coagulation factor XIII (FXIII-B) is a ~80 kDa glycoprotein containing two N-glycosylation sites.
32168410	1	59	gly	N-glycosylation	217:231	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	BACKGROUND The protective/inhibitory B subunits of coagulation factor XIII (FXIII-B) is a ~80 kDa glycoprotein containing two N-glycosylation sites.
32258897	7	33	gly	arm	1183:1185	arg1	outer arm fucose				outer arm fucose						N-glycans consisted of a mixture of bi-, tri-, and tetra-antennary glycans, some with N-acetyllactosamine extensions, but neither outer arm fucose nor α-galactose was detected.
29454068	0	45	gly	RACK1	19:23	arg1	O-GlcNAcylation	RACK1			O-GlcNAcylation	PUBTATOR		RACK1	10399		O-GlcNAcylation of RACK1 promotes hepatocellular carcinogenesis.
33629527	7	83	gly	N-glycosylation	1269:1283	arg2	N-glycosylation sites			N-glycosylation sites						sites	Detailed analysis of the HA N-glycan microheterogeneity showed an increasing variability and a higher complexity for N-glycosylation sites located closer to the head region of the molecule.
32764711	4	46	gly	linked	599:604	arg2	the E protein AND N-glycans	the E protein			N-glycans	Fterm		protein			In this regard, the main purpose of our study was to investigate the profile of N-glycans linked to the E protein of TBEV when grown in human neuronal cells and compare it to the profile of virus grown in tick cells.
30956133	5	15	gly	proteins	642:649	arg1	hypersialylation	proteins			hypersialylation	Fterm		proteins	6480		Functional ST6Gal-I in exomeres can be transferred to cells, resulting in hypersialylation of recipient cell-surface proteins including β1-integrin.
30956133	5	50	gly	hypersialylation	599:614	arg1	β1-integrin	β1-integrin				PUBTATOR		1-integrin	3688		Functional ST6Gal-I in exomeres can be transferred to cells, resulting in hypersialylation of recipient cell-surface proteins including β1-integrin.
30956133	5	50	gly	hypersialylation	599:614	arg1	recipient cell-surface proteins	recipient cell-surface proteins				Fterm		proteins	6480		Functional ST6Gal-I in exomeres can be transferred to cells, resulting in hypersialylation of recipient cell-surface proteins including β1-integrin.
29920571	6	33	gly	glycopeptides	998:1010	arg2	273 glycopeptides			273 glycopeptides						glycopeptides	In all, 273 glycopeptides corresponding to 94 glycoproteins were identified from only 2 μL human serum.
29920571	6	35	gly	glycoproteins	1032:1044	arg1	94 glycoproteins	94 glycoproteins				Fterm		glycoproteins			In all, 273 glycopeptides corresponding to 94 glycoproteins were identified from only 2 μL human serum.
34192331	3	36	gly	attached	433:440	arg1	the IgG-Fc region AND the N-glycan structures			the IgG-Fc region	the N-glycan structures					region	While the N-glycan structures attached to the IgG-Fc region are generally heterogenous, IgGs engineered to be homogenously glycosylated with functional N-glycans may improve the efficacy of antibodies.
28303575	2	37	gly	glycosylation	321:333	arg2	its N-linked glycosylation site			its N-linked glycosylation site						site	Human monocyte populations express Dectin-1 isoforms A and B, which differ by the presence of a stalk region and its N-linked glycosylation site.
36303733	7	73	gly	SARS-CoV-2	1343:1352	arg1	a substantially modified glycan profile	SARS			a substantially modified glycan profile	OGER		SARS	P49591		Blocking specific enzymes resulted in a substantially modified glycan profile of SARS-CoV-2.
29045410	6	29	gly	glycosylation	1260:1272	arg2	predicted N-linked glycosylation sites			predicted N-linked glycosylation sites						sites	As previously reported for men, time to peak diversity in env-gp120 in women was positively associated with time to CD4+ cell count below 200 (P = 0.017), and the number of predicted N-linked glycosylation sites generally increased over time, followed by a plateau or decline, with the majority of changes localized to the V1-V2 region.
29917166	2	18	gly	glycoproteins	261:273	arg1	osteopontin	osteopontin				PUBTATOR		osteopontin	6696		Studies have demonstrated that small integrin‑binding ligand N‑linked glycoproteins (SIBLINGs), particularly bone sialoprotein (BSP) and osteopontin (OPN), are involved in neoplastic growth and metastasis.
29917166	2	18	gly	glycoproteins	261:273	arg1	bone sialoprotein	bone sialoprotein				PUBTATOR		bone sialoprotein	3381		Studies have demonstrated that small integrin‑binding ligand N‑linked glycoproteins (SIBLINGs), particularly bone sialoprotein (BSP) and osteopontin (OPN), are involved in neoplastic growth and metastasis.
29917166	2	18	gly	glycoproteins	261:273	arg1	small integrin‑binding ligand N‑linked glycoproteins	small integrin‑binding ligand N‑linked glycoproteins				Fterm		glycoproteins			Studies have demonstrated that small integrin‑binding ligand N‑linked glycoproteins (SIBLINGs), particularly bone sialoprotein (BSP) and osteopontin (OPN), are involved in neoplastic growth and metastasis.
32441515	3	27	gly	glycoproteins	523:535	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Mass spectrometry (MS) is one of the most efficient tools used in the current studies of glycoproteins and structure of their glycoforms.
34128680	9	21	gly	glycosylation	1975:1987	arg1	peptide self-assembly			peptide self-assembly						peptide	These observations add to the understanding of the effect of glycosylation on peptide self-assembly and should guide future efforts to develop biomaterials from synthetic β-sheet fibrillizing glycopeptides.
34128680	9	32	gly	glycopeptides	2106:2118	arg2	synthetic β-sheet fibrillizing glycopeptides			synthetic β-sheet fibrillizing glycopeptides						glycopeptides	These observations add to the understanding of the effect of glycosylation on peptide self-assembly and should guide future efforts to develop biomaterials from synthetic β-sheet fibrillizing glycopeptides.
30032777	7	17	gly	glycopeptides	1291:1303	arg2	low-abundance N-linked glycopeptides			low-abundance N-linked glycopeptides						glycopeptides	Therefore, this hydrophilic MOP-coated adsorbent would be applied in the enrichment and identification of low-abundance N-linked glycopeptides in complicated biological samples.
30296064	1	32	gly	proteins	132:139	arg1	O-GlcNAc	proteins			O-GlcNAc	Fterm		proteins			The modification of proteins with O-linked N-acetylglucosamine ( O-GlcNAc) by the enzyme O-GlcNAc transferase (OGT) has emerged as an important regulator of cellular physiology.
30296064	1	32	gly	proteins	132:139	arg1	O-linked N-acetylglucosamine	proteins			O-linked N-acetylglucosamine	Fterm		proteins			The modification of proteins with O-linked N-acetylglucosamine ( O-GlcNAc) by the enzyme O-GlcNAc transferase (OGT) has emerged as an important regulator of cellular physiology.
34450331	5	108	gly	glycoprotein	921:932	arg1	glycoprotein extracts	glycoprotein extracts				Fterm		glycoprotein			The method was then applied to glycoprotein extracts from LLC-MK2 epithelial cells upon treatment with dithiothreitol to induce endoplasmic reticulum stress and promote protein misfolding.
28939828	7	14	gly	glycoprotein	1403:1414	arg1	glycoprotein substrates	glycoprotein substrates				Fterm		glycoprotein			These findings provide structural insights into the working mechanism whereby UGGT operates as a folding-sensor against a variety of glycoprotein substrates through its flexible modular structure possessing extended hydrophobic surfaces for the recognition of unfolded substrates.
29869806	1	19	gly	glycosylation	262:274	arg1	proteins	proteins				Fterm		proteins			Congenital disorders of glycosylation (CDG) are rare autosomal genetic diseases affecting the glycosylation of proteins and lipids.
30487799	3	32	gly	glycosylation	473:485	arg2	each glycosylation site			each glycosylation site						site	In order to characterize these site-specific N-glycans, we conducted the first study of the N-glycans of each glycosylation site of the grass carp serum IgM.
30487799	3	52	gly	site	487:490	arg1	the N-glycans			site	the N-glycans					site	In order to characterize these site-specific N-glycans, we conducted the first study of the N-glycans of each glycosylation site of the grass carp serum IgM.
34631661	11	1	gly	sites	2032:2036	arg1	HEK-Spike	HEK			sites	PUBTATOR		HEK	2042		The relatively higher amount of high-mannose abundant sites (N17, N234, N343, N616, N709, N717, N801, and N1134) on HEK-Spike suggests that glycan-shielding may differ among the two constructs.
34631661	11	59	gly	HEK-Spike	2094:2102	arg1	high-mannose abundant sites	HEK			high-mannose abundant sites	PUBTATOR		HEK	2042		The relatively higher amount of high-mannose abundant sites (N17, N234, N343, N616, N709, N717, N801, and N1134) on HEK-Spike suggests that glycan-shielding may differ among the two constructs.
33554253	8	57	gly	glycosylation	1465:1477	arg1	the recombinant produced IgGs	the recombinant produced IgGs				Cterm		IgGs	16059		The distribution of the different detected glycoforms in our rRb-IgG N-glycan analysis is in agreement with previous studies on recombinant human IgG N-glycans, confirming the hypothesis that the host cell defines the glycosylation of the recombinant produced IgGs.
33629527	8	32	gly	N-glycosylated	1386:1399	arg2	site N73	NA		N73		Cterm		NA	4758	N73	In contrast, NA was found to be exclusively N-glycosylated at site N73.
31516400	7	33	gly	N-glycoproteins	1305:1319	arg1	more than 7200 N-glycoproteins	more than 7200 N-glycoproteins				Fterm		N-glycoproteins			RESULTS The N-GlycositeAtlas database contains more than 30,000 glycosite-containing peptides (representing > 14,000 N-glycosylation sites) from more than 7200 N-glycoproteins from different biological sources including human-derived tissues, body fluids and cell lines from over 100 studies.
31516400	7	50	gly	N-glycosylation	1262:1276	arg2	more than 30,000 glycosite-containing peptides			more than 30,000 glycosite-containing peptides						peptides	RESULTS The N-GlycositeAtlas database contains more than 30,000 glycosite-containing peptides (representing > 14,000 N-glycosylation sites) from more than 7200 N-glycoproteins from different biological sources including human-derived tissues, body fluids and cell lines from over 100 studies.
31516400	7	50	gly	N-glycosylation	1262:1276	arg2	representing > 14,000 N-glycosylation sites			representing > 14,000 N-glycosylation sites						sites	RESULTS The N-GlycositeAtlas database contains more than 30,000 glycosite-containing peptides (representing > 14,000 N-glycosylation sites) from more than 7200 N-glycoproteins from different biological sources including human-derived tissues, body fluids and cell lines from over 100 studies.
31281932	2	51	gly	glycosylation	322:334	arg1	the protein	the protein				Fterm		protein			Therapeutic efficacy and serum half-life are particularly important, and these properties rely heavily on the glycosylation state of the protein.
28368034	6	75	gly	modified	929:936	arg1	The plant-based S-hyIgA AND plant-specific N-linked sugar chains	The plant-based S-hyIgA			plant-specific N-linked sugar chains	Cterm		IgA	P11912		The plant-based S-hyIgA exhibited antigen binding, and was modified with plant-specific N-linked sugar chains.
29531238	6	18	gly	glycoproteins	1081:1093	arg1	40 glycoproteins	40 glycoproteins				Fterm		glycoproteins			nsSNVs causing loss of 24 NLGs on 23 glycoproteins and nsSNVs creating 41 NLGs on 40 glycoproteins are identified in three or more cancers.
29531238	6	52	gly	glycoproteins	1033:1045	arg1	23 glycoproteins	23 glycoproteins				Fterm		glycoproteins			nsSNVs causing loss of 24 NLGs on 23 glycoproteins and nsSNVs creating 41 NLGs on 40 glycoproteins are identified in three or more cancers.
30535277	10	39	gly	fucosylated	1759:1769	arg1	fucosylated N/O-glycans				fucosylated N/O-glycans						This study could facilitate the discovery of biomarkers for HC and HCC based on precise alterations of fucosylated N/O-glycans in saliva.
30158294	11	88	gly	non-glycosylated	1726:1741	arg1	non-glycosylated SERINC5	non-glycosylated SERINC5				PUBTATOR		SERINC5	256987		We conclude that although not required for restrictive-activity or Nef-sensitivity, N-linked glycosylation is important for maintaining the steady-state expression of SERINC5 and that non-glycosylated SERINC5 is likely subjected to a quality-control mechanism that induces its proteasomal degradation.IMPORTANCE SERINC5 is a member of a family of multi-pass transmembrane proteins that inhibit the infectivity of retroviruses including HIV-1.
33177111	8	46	gly	CD55	1151:1154	arg1	The O-glycan profile	CD55			The O-glycan profile	OGER		CD55	P08174		The O-glycan profile of CD55 demonstrated a shift in abundance to nonsialylated core 1 and monosialylated core 2 at the expense of the disialylated core 2 structure after ST3GAL1 silencing.
33177111	8	47	gly	nonsialylated	1193:1205	arg1	nonsialylated core 1				nonsialylated core 1						The O-glycan profile of CD55 demonstrated a shift in abundance to nonsialylated core 1 and monosialylated core 2 at the expense of the disialylated core 2 structure after ST3GAL1 silencing.
33177111	8	52	gly	monosialylated	1218:1231	arg1	monosialylated core 2				monosialylated core 2						The O-glycan profile of CD55 demonstrated a shift in abundance to nonsialylated core 1 and monosialylated core 2 at the expense of the disialylated core 2 structure after ST3GAL1 silencing.
33177111	8	61	gly	disialylated	1262:1273	arg1	the disialylated core 2 structure				the disialylated core 2 structure						The O-glycan profile of CD55 demonstrated a shift in abundance to nonsialylated core 1 and monosialylated core 2 at the expense of the disialylated core 2 structure after ST3GAL1 silencing.
33030205	2	24	gly	glycans	382:388	arg1	their active sites			their active sites	their active sites		Site			sites	A class of peptidases, referred to as glycopeptidases or O-glycopeptidases, circumvent the protective effect of glycans against proteolysis by accommodating the glycans in their active sites as specific features of substrate recognition.
32171833	7	76	gly	composition	1281:1291	arg1	the six legume polysaccharides			position	the six legume polysaccharides					position	Composition analysis indicated that the six legume polysaccharides were obviously different in moisture, ash, neutral sugar, uronic acid, total phenolic content, total flavonoid content, amino acid composition and monosaccharide composition.
32842538	2	43	gly	N-glycosylated	290:303	arg1	envelope (E) protein	envelope (E) protein				Fterm		protein			Two Zika virus (ZIKV) structural proteins, the pre-membrane (prM) and envelope (E) protein are N-glycosylated.
32842538	2	43	gly	N-glycosylated	290:303	arg1	Two Zika virus (ZIKV) structural proteins	Two Zika virus (ZIKV) structural proteins				Fterm		proteins			Two Zika virus (ZIKV) structural proteins, the pre-membrane (prM) and envelope (E) protein are N-glycosylated.
28528272	9	19	gly	glycosylated	1282:1293	arg1	glycosylated prolegumain	glycosylated prolegumain				Fterm		prolegumain			While glycosylated prolegumain was able to autoactivate, the unglycosylated form was not, and addition of glycosaminoglycans did not facilitate autoactivation of unglycosylated prolegumain.
28528272	9	51	gly	unglycosylated	1438:1451	arg1	unglycosylated prolegumain	unglycosylated prolegumain				Fterm		prolegumain			While glycosylated prolegumain was able to autoactivate, the unglycosylated form was not, and addition of glycosaminoglycans did not facilitate autoactivation of unglycosylated prolegumain.
32469948	9	55	gly	glycoproteins	1416:1428	arg1	human glycoproteins	human glycoproteins				Fterm		glycoproteins			This study lays a cornerstone of glycosylation engineering in K. marxianus toward producing human glycoproteins.
32965048	6	59	gly	peptide	1302:1308	arg1	the GlcNAc-containing site-determining peptide backbone				the GlcNAc-containing site-determining peptide backbone						For this, we used b/y product ion pairs from the GlcNAc-containing site-determining peptide backbone and structure-diagnostic product ions from the N-glycan moieties, respectively.
29753090	10	40	gly	MUC10	1285:1289	arg1	NeuAcα2-3Galβ1-3GalNAc	MUC10			NeuAcα2-3Galβ1-3GalNAc	PUBTATOR		MUC10	17830		A major O-glycan of MUC10 was determined to be NeuAcα2-3Galβ1-3GalNAc.
29753090	10	40	gly	MUC10	1285:1289	arg1	A major O-glycan	MUC10			A major O-glycan	PUBTATOR		MUC10	17830		A major O-glycan of MUC10 was determined to be NeuAcα2-3Galβ1-3GalNAc.
31333671	8	13	gly	glycosylation	1000:1012	arg2	N-linked glycosylation motifs			N-linked glycosylation motifs						motifs	Many IgE sequences contained N-linked glycosylation motifs.
32915505	0	17	gly	Glycans	40:46	arg1	the SARS-CoV-2 Spike Protein	Spike Protein			Glycans	PUBTATOR		Spike Protein	43740568		Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins.
32915505	0	17	gly	Glycans	40:46	arg1	the Receptor Binding Domain	Domain			Glycans	Fterm		Domain			Structural Characterization of N-Linked Glycans in the Receptor Binding Domain of the SARS-CoV-2 Spike Protein and their Interactions with Human Lectins.
28778927	6	23	gly	glycosylation	1144:1156	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Most of the identified sequences contain predicted secretion leader sequences, N-linked glycosylation sites, and a putative site of tyrosine sulfation.
33340519	9	42	gly	glycosylation	1197:1209	arg1	SARS-CoV-2	SARS-CoV-2				OGER		SARS	P49591		We suggest that any role of glycosylation in the pathobiology of SARS-CoV-2 will lie beyond its immediate impact of receptor glycosylation on virus binding.
31308178	5	20	gly	sialylated	1110:1119	arg1	sialylated N-glycans				sialylated N-glycans						We provide evidence that cell surface-associated VEGFR2 displays sialylated N-glycans at Asn-247 and, in contrast, that the nearby sites Asn-145 and Asn-160 contain lower levels of sialylated N-glycans and higher levels of high-mannose N-glycans, respectively.
31308178	5	52	gly	sialylated	994:1003	arg1	sialylated N-glycans				sialylated N-glycans						We provide evidence that cell surface-associated VEGFR2 displays sialylated N-glycans at Asn-247 and, in contrast, that the nearby sites Asn-145 and Asn-160 contain lower levels of sialylated N-glycans and higher levels of high-mannose N-glycans, respectively.
32364434	10	51	gly	glycoprotein	2038:2049	arg1	glycoprotein therapeutics	glycoprotein therapeutics				Fterm		glycoprotein			This approach can automate the high-throughput screening of glycosylation on large sample sets of glycoproteins, including clinical glycan biomarkers and glycoprotein therapeutics.
32364434	10	99	gly	glycoproteins	1982:1994	arg1	clinical glycan biomarkers	glycoproteins			clinical glycan biomarkers	Fterm		glycoproteins			This approach can automate the high-throughput screening of glycosylation on large sample sets of glycoproteins, including clinical glycan biomarkers and glycoprotein therapeutics.
32364434	10	99	gly	glycoproteins	1982:1994	arg1	glycoproteins	glycoproteins			clinical glycan biomarkers	Fterm		glycoproteins			This approach can automate the high-throughput screening of glycosylation on large sample sets of glycoproteins, including clinical glycan biomarkers and glycoprotein therapeutics.
32364434	10	99	gly	glycoproteins	1982:1994	arg1	glycoproteins	glycoproteins			clinical glycan biomarkers	Fterm		glycoproteins			This approach can automate the high-throughput screening of glycosylation on large sample sets of glycoproteins, including clinical glycan biomarkers and glycoprotein therapeutics.
34192302	8	35	gly	found	1378:1382	arg1	M2BP AND tri-antennary and tetra-antennary N-glycans	M2BP			tri-antennary and tetra-antennary N-glycans	PUBTATOR		M2BP	3959		In F4-IP(+) M2BP, many branched structures, including tri-antennary and tetra-antennary N-glycans, were found.
32915505	5	52	gly	glycoprotein	904:915	arg1	the glycoprotein perspective	the glycoprotein perspective				Fterm		glycoprotein			Complementary experiments from the glycoprotein perspective or from the lectin's point of view have permitted to disentangle the specific interacting epitopes in each case.
32858085	6	56	gly	non-glycosylated	1016:1031	arg1	M9GN2-RNase and non-glycosylated RNase A	M9GN2-RNase and non-glycosylated RNase A				PUBTATOR		RNase A	6035		RESULTS HUGT1 slightly accelerated the folding of M9GN2-RNase and non-glycosylated RNase A as the same extent.
33273015	5	28	gly	Asn355/Asn391-glycosylation	574:600	arg2	Asn355/Asn391-glycosylation sites			Asn355/Asn391-glycosylation sites						sites	Occlusion of the Asn355/Asn391-glycosylation sites and the Asn323-/Asn483-glycans, located in the MPO dimerisation zone, was found to affect the local glycan processing, thereby providing a molecular basis of the site-specific nMPO glycosylation.
31879931	4	11	gly	N-glycopeptides	691:705	arg2	large complex N-glycopeptides			large complex N-glycopeptides						N-glycopeptides	Among them, the chemoenzymatic method based on an endoglycosidase-catalyzed ligation of free N-glycans and GlcNAc-tagged peptides is emerging as a highly efficient method for constructing large complex N-glycopeptides.
30712865	3	7	gly	glycoproteins	592:604	arg1	the SARS-CoV and MERS-CoV S glycoproteins	the SARS-CoV and MERS-CoV S glycoproteins				Fterm		glycoproteins			To understand the humoral immune response elicited upon natural infections with coronaviruses, we structurally characterized the SARS-CoV and MERS-CoV S glycoproteins in complex with neutralizing antibodies isolated from human survivors.
33577335	4	14	gly	glycoproteins	688:700	arg1	The obtained homogeneous glycoproteins	The obtained homogeneous glycoproteins				Fterm		glycoproteins			The obtained homogeneous glycoproteins allow for the demonstration of the stabilizing role of N-glycans during the folding step.
31783892	3	37	gly	glycosylated	508:519	arg1	the glycosylated residue			the glycosylated residue						residue in	Considering that the codon of Asparagine is aac or aat, we wondered if the adenine base editor (ABE), which induces a·t to g·c conversion at specific site, could be used to reduce PD-1 suppression by changing the glycosylated residue in CAR-T cells.
32699088	10	44	gly	glycosylation	1903:1915	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Sequence analyses of the influenza A virus (IAV) surface antigen neuraminidase (NA or N) showed that the conservation of N-linked glycosylation sites on the NA enzymatic head domain differs by IAV subtype (H1N1 versus H3N2) and species of origin, with human-derived IAVs possessing the most variability.
31041386	7	38	gly	O-GlcNAc	1006:1013	arg1	chromatin-associated proteins	proteins			O-GlcNAc	Fterm		proteins			Analysis of resulting next-generation DNA sequencing data revealed that O-GlcNAc on chromatin-associated proteins at most genomic loci is processed with a half-life in hours.
28187132	6	29	gly	glycans	1095:1101	arg1	IgA1	IgA1			glycans	PUBTATOR		IgA1	P01876		Moreover, rs13226913 represents a strong cis-eQTL for C1GALT1 that encodes the key enzyme responsible for the transfer of galactose to O-linked glycans on IgA1.
34717971	2	17	gly	glycoprotein	489:500	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			The low yields of the soluble form of the trimeric spike (S) glycoprotein from SARS-CoV-2 has prompted advances in protein engineering that have greatly enhanced the stability and yields of the glycoprotein.
34717971	2	31	gly	glycoprotein	356:367	arg1	the trimeric spike (S) glycoprotein	the trimeric spike (S) glycoprotein				Fterm		glycoprotein			The low yields of the soluble form of the trimeric spike (S) glycoprotein from SARS-CoV-2 has prompted advances in protein engineering that have greatly enhanced the stability and yields of the glycoprotein.
31616924	9	91	gly	disialylated	1526:1537	arg1	the branched disialylated structure				the branched disialylated structure						Modeling suggested that C-terminal glycosylation, particularly the branched disialylated structure, could interact across domains including the receptor-binding domain.
32038294	0	85	gly	Increase	6:13	arg1	O-GlcNAc	Increase			O-GlcNAc	Fterm		Increase			Acute Increase in O-GlcNAc Improves Survival in Mice With LPS-Induced Systemic Inflammatory Response Syndrome.
33377107	2	7	gly	glycoproteins	352:364	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycoproteomics analyzes intact glycopeptides to examine site-specific microheterogeneity of glycoproteins.
33377107	2	13	gly	microheterogeneity	330:347	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycoproteomics analyzes intact glycopeptides to examine site-specific microheterogeneity of glycoproteins.
33377107	2	23	gly	glycopeptides	291:303	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Glycoproteomics analyzes intact glycopeptides to examine site-specific microheterogeneity of glycoproteins.
31142619	3	23	gly	glycoproteins	602:614	arg1	intracellular parasite glycoproteins	intracellular parasite glycoproteins				Fterm		glycoproteins			The cyst wall is known to have abundant O-linked N-acetylgalactosamine glycans, but the existing metabolic labeling methods do not allow selective labeling of intracellular parasite glycoproteins without labeling of host glycans.
31800099	6	19	gly	glycosylation	998:1010	arg1	human lipoprotein homeostasis	human lipoprotein homeostasis				Fterm		lipoprotein			Our study provides insight into the role of protein glycosylation in human lipoprotein homeostasis.
31019513	5	71	gly	glycosylation	1061:1073	arg2	12 N-linked glycosylation sites			12 N-linked glycosylation sites						sites	CD22 is highly glycosylated, containing 12 N-linked glycosylation sites on its extracellular domain, the function of which remain to be resolved.
31019513	5	106	gly	glycosylated	1024:1035	arg1	CD22	CD22				PUBTATOR		CD22	933		CD22 is highly glycosylated, containing 12 N-linked glycosylation sites on its extracellular domain, the function of which remain to be resolved.
34495528	7	10	gly	glycoproteins	1008:1020	arg1	some glycoproteins	some glycoproteins				Fterm		glycoproteins			Despite the N-glycan-dependent folding process, some glycoproteins do not fold correctly, and these misfolded glycoproteins are destined to degradation by proteasomes in the cytosol.
34495528	7	58	gly	glycoproteins	1065:1077	arg1	these misfolded glycoproteins	these misfolded glycoproteins				Fterm		glycoproteins			Despite the N-glycan-dependent folding process, some glycoproteins do not fold correctly, and these misfolded glycoproteins are destined to degradation by proteasomes in the cytosol.
33993882	4	30	gly	glycoproteins	715:727	arg1	glycoproteins' functions	glycoproteins' functions				Fterm		glycoproteins			Changes in N-glycosylation can ultimately affect glycoproteins' functions, which will have an impact on cell machinery.
30762425	5	12	gly	β-catenin	998:1006	arg1	the O-GlcNAcylation	-catenin			the O-GlcNAcylation	PUBTATOR		-catenin	1499		Moreover, the O-GlcNAcylation of β-catenin promoted the proliferation, colony formation, and repressed the induction of apoptosis in HEP-G2 and HuH-7 cells.
29718541	2	15	gly	nonglycoprotein	337:351	arg1	the bona fide nonglycoprotein Nox5	the bona fide nonglycoprotein Nox5				Fterm		nonglycoprotein			Here, we show that the bona fide nonglycoprotein Nox5, a transmembrane superoxide-producing NADPH oxidase, is transported to the cell surface in a manner resistant to co-expression of Sar1 (H79G), a GTP-fixed mutant of the small GTPase Sar1, which blocks COPII vesicle fission from the ER.
28591079	7	35	gly	predicted-N-linked-glycosylation	982:1013	arg2	predicted-N-linked-glycosylation sites			predicted-N-linked-glycosylation sites						sites	The frequencies of sequences with predicted-N-linked-glycosylation sites and C-X-C chemokine receptor type 4 were comparable in aqueous humor and plasma of all five patients.
32384234	3	94	gly	glycopeptides	777:789	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	To address these issues, we explored the use of negative-mode MS2/MS3 fragmentation to supplement current nanoLC-MS2-based sequencing and identification of intact glycopeptides largely performed in positive mode.
28657654	5	9	gly	peptide	1092:1098	arg1	O-GlcNAc peptide abundance				O-GlcNAc peptide abundance						Moreover, alteration of O-GlcNAc peptide abundance could be attributed more to O-GlcNAcylation level than to protein level changes.
34869209	13	35	gly	O-glycosites	1852:1863	arg2	O-glycosites			O-glycosites						O-glycosites	Concerning O-glycosylation, our analyses revealed that HEK293 recombinant proteins possessed a range of O-glycosites with compositions consistent with Core type 1 and 2 glycans.
29793953	4	134	gly	unglycosylated	889:902	arg1	Endogenous NTCP protein	Endogenous NTCP protein				PUBTATOR		Endogenous NTCP protein	6554		Endogenous NTCP protein from differentiated HepaRG cells was unglycosylated despite wild-type coding sequence.
32441515	5	55	gly	glycoforms	900:909	arg1	proteins	proteins				Fterm		proteins			Here we report the utility of CE modulation for qualitative and quantitative analysis of site- and structure-specific glycoforms of proteins.
32825463	3	23	gly	fucosylated	285:295	arg1	fucosylated glycans				fucosylated glycans						For detection and functional modulation of fucosylated glycans, chemical biology approaches using fucose (Fuc) analogs are useful.
30991145	0	41	gly	glycosylation	9:21	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	N-linked glycosylation sites in G protein of infectious hematopoietic necrosis virus (IHNV) affect its virulence and immunogenicity in rainbow trout.
33462887	6	16	gly	glycosylation	1133:1145	arg2	glycosylation sites			glycosylation sites						sites	We find that rather than the mass or length of glycoprotein modifications, the stability of DC-SIGN is better correlated with the number of glycosylation sites.
33462887	6	35	gly	glycoprotein	1040:1051	arg1	glycoprotein modifications	glycoprotein modifications				Fterm		glycoprotein			We find that rather than the mass or length of glycoprotein modifications, the stability of DC-SIGN is better correlated with the number of glycosylation sites.
33908012	1	13	gly	glycosylated	222:233	arg1	glycosylated therapeutic proteins	glycosylated therapeutic proteins				Fterm		proteins			The presence of sialic acids is one characteristic of glycosylated therapeutic proteins.
29339807	3	59	gly	glycoprotein	543:554	arg1	glycoprotein fucosylation				glycoprotein fucosylation						While the relationship between the abnormal expression of FUT8 and glycoprotein fucosylation in different prostate cancer cells has been demonstrated, there is no evidence that shows dysregulated fucosylation might be involved in prostate cancer progression from androgen-dependent to castration-resistant prostate cancer.
29339411	0	8	gly	Fragment	145:152	arg1	Rhamnose Fragment Rearrangement				Rhamnose Fragment Rearrangement						Flagellin Glycoproteomics of the Periodontitis Associated Pathogen Selenomonas sputigena Reveals Previously Not Described O-glycans and Rhamnose Fragment Rearrangement Occurring on the Glycopeptides.
29237830	0	55	gly	Glycosylation	74:86	arg1	Dystroglycan				Dystroglycan						Axl Can Serve as Entry Factor for Lassa Virus Depending on the Functional Glycosylation of Dystroglycan.
28614667	8	57	gly	N130	1586:1589	arg1	a single GlcNAc residue	CTR		N130	a single GlcNAc residue			CTR	799	N130	Characterization of peptide-binding affinities of purified N → Q CTR ECD glycan site mutants combined with PNGase F and Endo H treatment strategies and mass spectrometry to define the glycan species indicated that a single GlcNAc residue at CTR N130 was responsible for the peptide affinity enhancement.
33116178	6	32	gly	motifs	1052:1057	arg1	core fucosylation				core fucosylation						All enzymes were localized in the plant Golgi apparatus, which allowed us to identify the SmFucTs involved in core fucosylation and the synthesis of complex antennary glycan motifs.
33116178	6	35	gly	fucosylation	993:1004	arg1	complex antennary glycan motifs			complex antennary glycan motifs							All enzymes were localized in the plant Golgi apparatus, which allowed us to identify the SmFucTs involved in core fucosylation and the synthesis of complex antennary glycan motifs.
29681862	9	84	gly	synthase	1863:1870	arg1	increased O-GlcNAcylation	endothelial nitric oxide synthase			increased O-GlcNAcylation	PUBTATOR		endothelial nitric oxide synthase	24600		PVAT from high sugar diet-fed rats for 12 weeks exhibited decreased NO formation, reduced expression of endothelial nitric oxide synthase (eNOS) and increased O-GlcNAcylation of eNOS.
29681862	9	97	gly	eNOS	1912:1915	arg1	increased O-GlcNAcylation	eNOS			increased O-GlcNAcylation	PUBTATOR		eNOS	24600		PVAT from high sugar diet-fed rats for 12 weeks exhibited decreased NO formation, reduced expression of endothelial nitric oxide synthase (eNOS) and increased O-GlcNAcylation of eNOS.
31067000	11	78	gly	glycosylation	1622:1634	arg1	the IgG ACPA V domain			the IgG ACPA V domain						domain	CONCLUSION Extensive glycosylation of the IgG ACPA V domain is present in a subset of predisposed FDRs of Indigenous North American RA patients.
28614667	6	11	gly	removal	1058:1064	arg2	N-glycans AND N73	CTR ECD		N73, N125, and N130	N-glycans			CTR ECD	799	N73, N125, and N130	PNGase F-catalyzed removal of N-glycans at N73, N125, and N130 in the CTR ECD decreased peptide affinity ∼10-fold, whereas Endo H-catalyzed trimming of the N-glycans to single GlcNAc residues had no effect on peptide binding.
28614667	6	11	gly	removal	1058:1064	arg3	N-glycans AND N125	CTR ECD		N73, N125, and N130	N-glycans			CTR ECD	799	N73, N125, and N130	PNGase F-catalyzed removal of N-glycans at N73, N125, and N130 in the CTR ECD decreased peptide affinity ∼10-fold, whereas Endo H-catalyzed trimming of the N-glycans to single GlcNAc residues had no effect on peptide binding.
28614667	6	11	gly	removal	1058:1064	arg3	N-glycans AND N125	CTR ECD		N73, N125, and N130	N-glycans			CTR ECD	799	N73, N125, and N130	PNGase F-catalyzed removal of N-glycans at N73, N125, and N130 in the CTR ECD decreased peptide affinity ∼10-fold, whereas Endo H-catalyzed trimming of the N-glycans to single GlcNAc residues had no effect on peptide binding.
29465311	5	49	gly	glycoproteins	849:861	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			We found that p32 interacts with endoplasmic reticulum-anchored enzyme mannosyl-oligosaccharide glucosidase I (GCS1), an ER lumen-anchored glucosidase that is essential for the processing of N-linked glycoproteins, and reduces GCS1 in a lysosome-dependent manner.
30422384	6	47	gly	glycosylation	1053:1065	arg1	human proMMP-9	human proMMP-9				OGER		MMP-9			Moreover, we investigated the effect of glycosylation on proteolytic activation of human proMMP-9 with the use of zymography and dye-quenched gelatin cleavage analysis.
29045410	7	68	gly	system	1512:1517	arg1	composition			composition						position	These findings strongly suggest that the sex differences in HIV-1 disease progression attributed to immune system composition and sensitivities are not revealed by, nor do they impact, global patterns of viral evolution, the latter of which proceeds similarly in women and men.
33453284	3	1	gly	enzyme	508:513	arg1	antennae synthesis	enzyme			antennae synthesis	Fterm		enzyme			Two new papers present a detailed structural and mechanistic investigation of β1-3-N-acetylgucosaminyltransferase 2, a key enzyme in antennae synthesis.
34110173	5	0	gly	glycopeptide	1120:1131	arg2	the glycopeptide level			the glycopeptide level						glycopeptide	The site-specificity of N-glycans was revealed at the glycopeptide level by pGlyco software using different proteases.
31527085	6	31	gly	site	775:778	arg1	sOGT	sOGT			site	Cterm		sOGT	8473		Here, we performed LC-MS/MS and mutational analyses to seek the major O-GlcNAcylation site on sOGT.
33263330	2	58	gly	glycoproteins	267:279	arg1	Most glycoproteins	Most glycoproteins				Fterm		glycoproteins			Most glycoproteins contain N-linked glycosylation, a key post-translation modification that critically affects protein folding and stability and the binding kinetics underlying protein interactions.
30919021	4	42	gly	glycosylation	470:482	arg1	N113			N113				prolyl 4-hydroxylase subunit α1	18451	N113	Our studies show that glycosylation of N259, but not N113, by STT3B and magnesium transporter 1 (MAGT1) is augmented by VC.
30919021	4	42	gly	glycosylation	470:482	arg1	N259	P4HA1		N259				P4HA1	18451	N259	Our studies show that glycosylation of N259, but not N113, by STT3B and magnesium transporter 1 (MAGT1) is augmented by VC.
34343291	4	58	gly	glycan-glycoprotein	637:655	arg1	glycan-glycoprotein				glycan-glycoprotein						How such modifications affect the glycan-glycoprotein dynamics, and ultimately their function is not clearly understood to date.
29672582	0	57	gly	IgG4	24:27	arg1	N-glycans	IgG4			N-glycans	OGER		IgG4	P01861		Changes in N-glycans of IgG4 and its relationship with the existence of hypocomplementemia and individual organ involvement in patients with IgG4-related disease.
30834446	9	54	gly	glycoprotein	1728:1739	arg1	the IHNV glycoprotein	the IHNV glycoprotein				Fterm		glycoprotein			To gain structural insight into the inhibitory effect of these galectins on IHNV attachment to the zebrafish epithelial cells, we modeled Drgal3-L1 based on human galectin-3, as well as, the ectodomain of the IHNV glycoprotein.
34279906	4	30	gly	glycoprotein	894:905	arg1	remarkable glycoprotein structural details	remarkable glycoprotein structural details				Fterm		glycoprotein			To address this deficiency, we developed a new approach that joins native mass spectrometry with glycan exoglycosidase sequencing, the combination of which provides remarkable glycoprotein structural details.
30364948	2	30	gly	di-glycosylated	333:347	arg1	an unusually di-glycosylated bacteriocin	an unusually di-glycosylated bacteriocin				Fterm		bacteriocin			Glycocin F (GccF) is an unusually di-glycosylated bacteriocin produced in a lactic acid bacterium, Lactobacillus plantarum KW30 that has been shown to be resistant to extreme conditions.
30364948	2	30	gly	di-glycosylated	333:347	arg1	Glycocin F	Glycocin F				Cterm		Glycocin F (GccF			Glycocin F (GccF) is an unusually di-glycosylated bacteriocin produced in a lactic acid bacterium, Lactobacillus plantarum KW30 that has been shown to be resistant to extreme conditions.
34192331	5	66	gly	IgGs	782:785	arg1	tri-antennary complex-type N-glycans	However, IgGs			tri-antennary complex-type N-glycans	Cterm		However, IgGs			However, IgGs with tri-antennary complex-type N-glycans have been generated using the N-glycan remodeling technique, suggesting that more branched N-glycans might be artificially attached.
28474196	3	82	gly	glycosylation	708:720	arg2	one N-linked glycosylation site			one N-linked glycosylation site						site	Sequence alignment and phylogenetic analysis revealed a high degree of conservation (76-96%) among most fish and other vertebrates, retaining one N-linked glycosylation site, one N-terminal acetylation site, 13 phosphorylation sites, one fructose-1,6-bisphosphatase (FBPase) active site, five metal-binding sites, four substrate-binding sites, and several AMP-binding sites.
30250045	6	61	gly	O-glycosylated	762:775	arg1	an O-glycosylated region			an O-glycosylated region						region	The epitope of SKM9-2 was identified as an O-glycosylated region, 893-SKSPSLVSLPT-903, in HEG1.
30250045	6	61	gly	O-glycosylated	762:775	arg1	The epitope			The epitope						epitope	The epitope of SKM9-2 was identified as an O-glycosylated region, 893-SKSPSLVSLPT-903, in HEG1.
31489629	5	12	gly	phosphoglycoprotein	905:923	arg1	matrix extracellular phosphoglycoprotein	matrix extracellular phosphoglycoprotein				OGER		matrix extracellular phosphoglycoprotein	Q9ES02		The gene and protein expressions of noncollagen proteins (BSP, bone sialoprotein; OCN, osteocalcin; OPN, osteopontin), p38 mitogen-activated protein kinase, and SIBLINGs (Small Integrin-Binding LIgand N-linked Glycoproteins) members (DMP1, dentine matrix protein 1, DSPP, dentin sialophosphoprotein, and MEPE, matrix extracellular phosphoglycoprotein) were detected by reverse-transcription quantitative polymerase chain reaction and western blot analysis.
34278967	7	59	gly	lectin	1469:1474	arg1	the carbohydrate specificity	lectin			the carbohydrate specificity	Fterm		lectin			Using structural information and determined N-glycan profile of S trimer, taking together with the carbohydrate specificity of lentil lectin, we provide a basis for the observed broad spectrum anti-SARS-CoV-2 activity.
34278967	7	69	gly	trimer	1401:1406	arg1	N-glycan profile	S trimer			N-glycan profile	Cterm		S trimer	43740568		Using structural information and determined N-glycan profile of S trimer, taking together with the carbohydrate specificity of lentil lectin, we provide a basis for the observed broad spectrum anti-SARS-CoV-2 activity.
29745666	4	18	gly	glycoproteins	664:676	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			Herein, we evaluated the use of sodium deoxycholate (SDC) acidic labile detergent to release N-glycans of glycoproteins derived from biological samples such as cancer cell lines.
32298658	4	56	gly	contain	673:679	arg1	avian-origin AND predominately complex-type glycans	avian-origin			predominately complex-type glycans	Fterm		avian-origin HAs	103990076		By contrast, poorly adapted avian-origin HAs contain predominately complex-type glycans, which have greater structural diversity.
30335141	6	26	gly	glycoprotein	1060:1071	arg1	a co-transfected secretory membrane glycoprotein	glycoprotein				Fterm		glycoprotein			In transfected Chinese hamster ovary AP-1 cells, the Leu515Phe mutant protein was correctly targeted to the TGN/post-Golgi vesicles, but its N-linked oligosaccharide maturation as well as that of a co-transfected secretory membrane glycoprotein, vesicular stomatitis virus G (VSVG) glycoprotein, was reduced compared to cells co-expressing SLC9A7 wild-type and VSVG.
30335141	6	91	gly	glycoprotein	1010:1021	arg1	a co-transfected secretory membrane glycoprotein	glycoprotein				Fterm		glycoprotein			In transfected Chinese hamster ovary AP-1 cells, the Leu515Phe mutant protein was correctly targeted to the TGN/post-Golgi vesicles, but its N-linked oligosaccharide maturation as well as that of a co-transfected secretory membrane glycoprotein, vesicular stomatitis virus G (VSVG) glycoprotein, was reduced compared to cells co-expressing SLC9A7 wild-type and VSVG.
30178920	3	8	gly	glycopeptides	861:873	arg2	only four and three glycopeptides			only four and three glycopeptides						glycopeptides	With IgG and horse radish peroxidase as the model glycoproteins, 28 and 17 glycopeptides could be identified respectively after enrichment with the monolith, only four and three glycopeptides could be obtained for direct analysis.
30178920	3	13	gly	glycoproteins	733:745	arg1	the model glycoproteins	the model glycoproteins				Fterm		glycoproteins			With IgG and horse radish peroxidase as the model glycoproteins, 28 and 17 glycopeptides could be identified respectively after enrichment with the monolith, only four and three glycopeptides could be obtained for direct analysis.
30178920	3	59	gly	glycopeptides	758:770	arg2	17 glycopeptides			17 glycopeptides						glycopeptides	With IgG and horse radish peroxidase as the model glycoproteins, 28 and 17 glycopeptides could be identified respectively after enrichment with the monolith, only four and three glycopeptides could be obtained for direct analysis.
31129958	6	3	gly	glycopeptide	997:1008	arg2	glycopeptide			glycopeptide						glycopeptide	We demonstrate the tool's efficiency by applying it to a set of 80 glycopeptide targets.
32681150	1	16	gly	glycosylated	118:129	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			The glycocalyx comprises glycosylated proteins and lipids and fcorms the outermost layer of cells.
30487799	5	16	gly	Asn-565	861:867	arg1	no glycan occupancy			Asn-565	no glycan occupancy			Grass Carp Serum IgM	Q15327	Asn-565	A striking decrease in the level of occupancy at the Asn-565 glycosite was observed in dimeric IgM compared to that in monomeric IgM, and no glycan occupancy of Asn-565 was observed in tetrameric IgM.
30487799	5	17	gly	occupancy	848:856	arg2	Asn-565			Asn-565				Grass Carp Serum IgM	Q15327	Asn-565	A striking decrease in the level of occupancy at the Asn-565 glycosite was observed in dimeric IgM compared to that in monomeric IgM, and no glycan occupancy of Asn-565 was observed in tetrameric IgM.
30487799	5	23	gly	observed	873:880	arg2	tetrameric IgM AND no glycan occupancy	tetrameric IgM			no glycan occupancy	PUBTATOR		IgM	P01871		A striking decrease in the level of occupancy at the Asn-565 glycosite was observed in dimeric IgM compared to that in monomeric IgM, and no glycan occupancy of Asn-565 was observed in tetrameric IgM.
30487799	5	46	gly	glycosite	761:769	arg2	the Asn-565 glycosite			the Asn-565 glycosite				Grass Carp Serum IgM	Q15327	Asn-565 glycosite	A striking decrease in the level of occupancy at the Asn-565 glycosite was observed in dimeric IgM compared to that in monomeric IgM, and no glycan occupancy of Asn-565 was observed in tetrameric IgM.
32809822	5	21	gly	core-fucosylated	997:1012	arg1	core-fucosylated N-glycans				core-fucosylated N-glycans						Routinely, over 75 N-glycan species can be detected from one microliter of serum in less than 6.5 h. Additionally, endoglycosidase F3 was applied to this workflow to identify core-fucosylated N-glycans and displayed the adaptability of this method for the determination of structural information.
33347638	7	72	gly	O-glycosylated	1395:1408	arg1	O-glycosylated proteins	O-glycosylated proteins				Fterm		proteins			RESULTS Lectin microarray analyses revealed that sera from patients with prostate cancer had a higher affinity for Jacalin, Amaranthus caudatus (ACA) lectin, and Maclura pomifera (MPA) lectin, compared with that from patients with benign prostatic diseases and normal subjects, suggesting that O-glycosylated proteins are more abundant in sera from patients with prostate cancer.
32452486	5	50	gly	glycoprotein	847:858	arg1	glycoprotein solution	glycoprotein solution				Fterm		glycoprotein			With one drop (1 μL) of glycoprotein solution, our lectin-SERS assay can detect as low as 10 ng IgG within two hours with high glycan specificity.
31498587	6	87	gly	glycopeptides	931:943	arg2	MUC1 glycopeptides			MUC1 glycopeptides						glycopeptides	The IgG antibodies recognized a wide range of MUC1 glycopeptides bearing diverse glycans.
30320380	3	85	gly	glycoproteins	608:620	arg1	the small integrin‑binding N‑linked glycoproteins family	the small integrin‑binding N‑linked glycoproteins family				Fterm		glycoproteins			Dentin sialophosphoprotein (DSPP), which is a member of the small integrin‑binding N‑linked glycoproteins family, has been implicated in malignant transformation and invasion of oral squamous cell carcinoma (OSCC).
33121334	2	13	gly	glycosylation	391:403	arg2	more N-linked glycosylation sequons			more N-linked glycosylation sequons							Some lead clinical candidates, including antibodies CAP256-VRC26.25, N6, PGT121, and VRC07-523, have one or more N-linked glycosylation sequons in their variable domains (Fvs) from somatic hypermutation, and these glycans increase chemical heterogeneity, complicating the manufacture of these antibodies as products.
33171078	2	58	gly	origin	277:282	arg1	galactose-alpha-1,3-galactose	origin			galactose-alpha-1,3-galactose	Fterm		origin			In particular, glycans of nonhuman origin, such as galactose-alpha-1,3-galactose (gal-α-gal) and N-glycolylneuraminic acid (NGNA), in the Fc region of therapeutic mAbs produced from murine cell lines carry a risk of immunogenicity.
33171078	2	58	gly	origin	277:282	arg1	N-glycolylneuraminic acid	origin			N-glycolylneuraminic acid	Fterm		origin			In particular, glycans of nonhuman origin, such as galactose-alpha-1,3-galactose (gal-α-gal) and N-glycolylneuraminic acid (NGNA), in the Fc region of therapeutic mAbs produced from murine cell lines carry a risk of immunogenicity.
33171078	2	58	gly	origin	277:282	arg1	glycans	origin			glycans	Fterm		origin			In particular, glycans of nonhuman origin, such as galactose-alpha-1,3-galactose (gal-α-gal) and N-glycolylneuraminic acid (NGNA), in the Fc region of therapeutic mAbs produced from murine cell lines carry a risk of immunogenicity.
33171078	2	72	gly	glycans	257:263	arg1	the Fc region			the Fc region	the Fc region		Site			region	In particular, glycans of nonhuman origin, such as galactose-alpha-1,3-galactose (gal-α-gal) and N-glycolylneuraminic acid (NGNA), in the Fc region of therapeutic mAbs produced from murine cell lines carry a risk of immunogenicity.
33600485	4	2	gly	occupancy	657:665	arg2	two glycosylation sites			two glycosylation sites						sites	Strains are biochemically distinguished by a characteristic three-band immunoblot pattern, defined by differences in the occupancy of two glycosylation sites on the prion protein (PrP).
33600485	4	15	gly	glycosylation	674:686	arg2	two glycosylation sites			two glycosylation sites						sites	Strains are biochemically distinguished by a characteristic three-band immunoblot pattern, defined by differences in the occupancy of two glycosylation sites on the prion protein (PrP).
28366604	8	4	gly	domain	1308:1313	arg1	a determinant			domain	a determinant					domain	Thus, we identified a determinant of the CD4i domain that might affect vaccine-elicited anti-V2 Ab and ADCC responses to SIVmac239.
28528272	14	15	gly	glycosylation	2023:2035	arg1	prolegumain	prolegumain				Fterm		prolegumain			In conclusion, glycosylation of prolegumain is necessary for correct processing to active forms and internalization, whereas the inhibitory property of cystatin E/M is independent of the glycosylation status.
34401920	8	5	gly	found	1070:1074	arg1	serum glycoproteins AND The N-glycan structures	serum glycoproteins			The N-glycan structures	Fterm		glycoproteins			The N-glycan structures found in serum glycoproteins were mainly Man2FucGlcNAc2 (67.9%).
34401920	8	5	gly	found	1070:1074	arg2	serum glycoproteins AND Man2FucGlcNAc2	serum glycoproteins			Man2FucGlcNAc2	Fterm		glycoproteins			The N-glycan structures found in serum glycoproteins were mainly Man2FucGlcNAc2 (67.9%).
34401920	8	29	gly	glycoproteins	1085:1097	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			The N-glycan structures found in serum glycoproteins were mainly Man2FucGlcNAc2 (67.9%).
30962950	10	10	gly	core-fucosylated	1663:1678	arg1	its core-fucosylated glycan				its core-fucosylated glycan						Our study suggests that serum cf-QSOX1-S and tumor QSOX1 levels are helpful for predicting recurrence in HCC patients, and its core-fucosylated glycan at Asn-130 is critical for the inhibitory effects of QSOX1-S on invasion and metastasis of HCC.
30962950	10	41	gly	Asn-130	1690:1696	arg1	its core-fucosylated glycan			Asn-130	its core-fucosylated glycan			QSOX1	104009	Asn-130	Our study suggests that serum cf-QSOX1-S and tumor QSOX1 levels are helpful for predicting recurrence in HCC patients, and its core-fucosylated glycan at Asn-130 is critical for the inhibitory effects of QSOX1-S on invasion and metastasis of HCC.
31237748	1	4	gly	proteins	181:188	arg1	Protein O-linked N-acetylglucosamine	proteins			Protein O-linked N-acetylglucosamine	Fterm		proteins			Protein O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of intracellular proteins that regulates several physiological and pathophysiological process, including response to various stressors.
33273015	10	68	gly	carrying	1762:1769	arg1	nMPO AND hyper-truncated glycans	nMPO			hyper-truncated glycans	Cterm		nMPO	P05164		Enzymatic trimming of the Asn355-/Asn391-glycans recapitulated the activity gain and showed that nMPO carrying hyper-truncated glycans at these positions exhibits increased thermal stability, polypeptide accessibility and ceruloplasmin-mediated inhibition potential relative to native nMPO.
33167210	0	52	gly	glycopeptide	16:27	arg2	High-throughput glycopeptide profiling			High-throughput glycopeptide profiling						glycopeptide	High-throughput glycopeptide profiling of prostate-specific antigen from seminal plasma by MALDI-MS.
30980499	7	49	gly	N-glycoproteins	896:910	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			In total, 708 proteins were identified by mass spectrometry in human TDM, of which 208 were N-glycoproteins with 318 identified glycosylation sites.
30980499	7	68	gly	glycosylation	932:944	arg2	318 identified glycosylation sites			318 identified glycosylation sites						sites	In total, 708 proteins were identified by mass spectrometry in human TDM, of which 208 were N-glycoproteins with 318 identified glycosylation sites.
34128680	4	10	gly	glycosylation	794:806	arg2	a site			site						site	Here, using transmission electron microscopy, fluorescence microscopy, and thioflavin T spectroscopy, we show that glycosylation at a site external to the fibrillization domain can alter the self-assembly pathway of a synthetic fibrillizing peptide, NSGSGQQKFQFQFEQQ (NQ11).
28657654	0	0	gly	proteins	105:112	arg1	altered O-GlcNAcylation	proteins			altered O-GlcNAcylation	Fterm		proteins			Quantitative proteomics identifies altered O-GlcNAcylation of structural, synaptic and memory-associated proteins in Alzheimer's disease.
32172531	1	17	gly	glycosylation	188:200	arg1	The human ether-a-go-go related gene (hERG)-encoded channel hERG	hERG		position 598,		PUBTATOR		hERG	2078	position 598,	The human ether-a-go-go related gene (hERG)-encoded channel hERG undergoes N-linked glycosylation at position 598, which is located in the unusually long S5-pore linker of the channel.
27728760	3	28	gly	glycoproteins	626:638	arg1	93 N-linked glycoproteins	93 N-linked glycoproteins				Fterm		glycoproteins			These glycoprotein identities and functionalities are compared with 93 N-linked glycoproteins identified on the surface of the parental cells.
27728760	3	61	gly	glycoprotein	552:563	arg1	These glycoprotein identities	These glycoprotein identities				Fterm		glycoprotein			These glycoprotein identities and functionalities are compared with 93 N-linked glycoproteins identified on the surface of the parental cells.
34944439	5	10	gly	residues	820:827	arg1	the T. forsythia&nbsp			residues in	the T. forsythia&nbsp					residues in	Of the two fucose residues in the T. forsythia&nbsp;O-glycan, the fucose linked to the reducing-end galactose was shown by mutational analysis to be l-fucose.
28490633	0	74	gly	glycoprotein	61:72	arg1	glycoprotein glucosyltransferase				glycoprotein glucosyltransferase						Single-particle electron microscopy structure of UDP-glucose:glycoprotein glucosyltransferase suggests a selectivity mechanism for misfolded proteins.
31631785	0	15	gly	glycoproteins	59:71	arg1	the envelope glycoproteins	the envelope glycoproteins				Fterm		glycoproteins			GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.
31875397	8	66	gly	glycosylated	1490:1501	arg1	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	This hybrid approach, coupling a shotgun method for neuropeptide discovery and targeted strategy for glycosylation characterization, enables the first report on glycosylated neuropeptides in crustaceans and the discovery of additional neuropeptides simultaneously.
30147262	8	33	gly	glycosylation	1282:1294	arg2	six potential N-linked glycosylation sites			six potential N-linked glycosylation sites						sites	Six of seven sequenced isolates have six potential N-linked glycosylation sites.
35140700	0	51	gly	Glycosylation	52:64	arg1	IgG	IgG				Cterm		IgG			Cytokines in the Immune Microenvironment Change the Glycosylation of IgG by Regulating Intracellular Glycosyltransferases.
30865517	2	37	gly	proteins	436:443	arg1	the O-GlcNAc modification	proteins			the O-GlcNAc modification	Fterm		proteins			Importantly, the O-GlcNAc modification of mitochondrial proteins has been identified as a potential mechanism to modulate metabolism under stress with both potentially beneficial and detrimental effects.
29499461	1	24	gly	glycoprotein	213:224	arg1	most glycoprotein based biotherapeutics	most glycoprotein based biotherapeutics				Fterm		glycoprotein			The carbohydrate moieties on the polypeptide chains in most glycoprotein based biotherapeutics and their biosimilars play essential roles in such major mechanisms of actions as antibody-dependent cell-mediated cytotoxicity, complement-dependent cytotoxicity, anti-inflammatory functions and serum clearance.
34670086	7	69	gly	glycosylation	1103:1115	arg2	several sites			several sites						sites	In contrast, several sites of O-linked glycosylation can be identified when RBD is expressed as a monomer, with T323 being almost completely occupied.
29779369	3	44	gly	glycoprotein	720:731	arg1	glycoprotein standards	glycoprotein standards				Fterm		glycoprotein			Duplex DiPyrO tags that differ in mass by 45.3 mDa were used to label the glycosylamine moieties of freshly released N-glycosylamines from glycoprotein standards and human serum proteins.
30718403	5	61	gly	glycosylation	828:840	arg2	a second N-linked glycosylation site			a second N-linked glycosylation site						site	The most striking differences were observed for three substitutions (Q25R, Q40R, and P68T), with P68T generating a second N-linked glycosylation site (N66) in addition to an invariant N32 encoded by all chimpanzee CD4 alleles.
32764711	7	31	gly	glycopeptides	1222:1234	arg2	the glycopeptides			the glycopeptides						glycopeptides	The observed pattern was supported by examination of the glycopeptides, providing additional information about the glycosylation site in the E protein.
32764711	7	70	gly	glycosylation	1280:1292	arg2	the glycosylation site			the glycosylation site						site	The observed pattern was supported by examination of the glycopeptides, providing additional information about the glycosylation site in the E protein.
27912985	7	22	gly	N-glycosylation	1289:1303	arg2	all N-glycosylation sites			all N-glycosylation sites						sites	Mosquito membrane feeding assays also revealed improved functional immunogenicity of SYN Pfs48/45 (N-glycosylation sites intact) as compared to MUT1 or MUT2 Pfs48/45 DNA plasmids (all N-glycosylation sites mutated).
27912985	7	30	gly	N-glycosylation	1204:1218	arg2	N-glycosylation sites intact			N-glycosylation sites intact						sites	Mosquito membrane feeding assays also revealed improved functional immunogenicity of SYN Pfs48/45 (N-glycosylation sites intact) as compared to MUT1 or MUT2 Pfs48/45 DNA plasmids (all N-glycosylation sites mutated).
27912985	7	76	gly	plasmids	1275:1282	arg1	all N-glycosylation sites			all N-glycosylation sites						sites	Mosquito membrane feeding assays also revealed improved functional immunogenicity of SYN Pfs48/45 (N-glycosylation sites intact) as compared to MUT1 or MUT2 Pfs48/45 DNA plasmids (all N-glycosylation sites mutated).
30881698	11	4	gly	sialylated	1572:1581	arg1	more sialylated glycans				more sialylated glycans						The immobilized enzyme released a higher abundance of neutral glycans from whey proteins, while the free enzyme released more sialylated glycans, determined by nano-LC Chip Q-ToF MS.
31913636	7	37	gly	glycoproteins	1534:1546	arg1	re-engineering therapeutic glycoproteins	re-engineering therapeutic glycoproteins				Fterm		glycoproteins			This reaction suggests that under certain experimental conditions, some glycoproteins can organize self-glycan addition, highlighting a remarkable self-assembly principle that may prove useful for re-engineering therapeutic glycoproteins such as influenza HA or HIV Env, where glycan sequence and structure can markedly affect bioactivity and vaccine efficacy.
31913636	7	37	gly	glycoproteins	1534:1546	arg1	HIV Env	HIV Env				PUBTATOR		HIV Env	100616444		This reaction suggests that under certain experimental conditions, some glycoproteins can organize self-glycan addition, highlighting a remarkable self-assembly principle that may prove useful for re-engineering therapeutic glycoproteins such as influenza HA or HIV Env, where glycan sequence and structure can markedly affect bioactivity and vaccine efficacy.
31913636	7	66	gly	glycoproteins	1382:1394	arg1	some glycoproteins	some glycoproteins				Fterm		glycoproteins			This reaction suggests that under certain experimental conditions, some glycoproteins can organize self-glycan addition, highlighting a remarkable self-assembly principle that may prove useful for re-engineering therapeutic glycoproteins such as influenza HA or HIV Env, where glycan sequence and structure can markedly affect bioactivity and vaccine efficacy.
29093093	4	0	gly	glycoprotein	654:665	arg1	the S glycoprotein trimer	the S glycoprotein trimer				Fterm		glycoprotein			We report here the 3.5-Å-resolution cryo-electron microscopy structure of the S glycoprotein trimer from the pathogenic porcine deltacoronavirus (PDCoV), which belongs to the recently identified Deltacoronavirus genus.
30476078	2	27	gly	fucosylated	418:428	arg1	the fucosylated Lewisx antigen				the fucosylated Lewisx antigen						Specifically, in colorectal cancer cells, increased levels of the fucosylated Lewisx antigen are attributed to the deregulated expression of pertinent fucosyltransferases, like fucosyltransferase 4 (FUT4) and fucosyltransferase 9 (FUT9).
32569746	5	27	gly	found	874:878	arg2	cell envelope surface-layer proteins AND N-glycans	cell envelope surface-layer proteins			N-glycans	Fterm		proteins			In archaea, N-glycans are mainly found on cell envelope surface-layer proteins, archaeal flagellins and pili.
33998969	2	49	gly	glycosylation	348:360	arg2	additional N-linked glycosylation site			additional N-linked glycosylation site						site	Introduction of additional N-linked glycosylation site into proposed location in the protein causes to its hyper-glycosylation and can enhance the protein characteristics to provide promising prospects in treatment.
30054538	9	65	gly	sialylation	1661:1671	arg1	PrPSc N-linked glycans				PrPSc N-linked glycans						To test whether the microglial response is mediated by carbohydrate epitopes on PrPSc surface, the levels of sialylation of PrPSc N-linked glycans was altered by treatment of purified PrPSc with neuraminidase.
32384234	2	14	gly	glycopeptides	460:472	arg2	single glycopeptides			single glycopeptides						glycopeptides	Among these, the ability to efficiently sequence the peptide backbone for de novo identification, delineating multiple N- and O-glycosylation sites on single glycopeptides, and deriving more glycan structure information to discriminate isomeric glycoforms are well acknowledged practical problems to be tackled.
32384234	2	23	gly	O-glycosylation	428:442	arg2	multiple N- and O-glycosylation sites			multiple N- and O-glycosylation sites						sites	Among these, the ability to efficiently sequence the peptide backbone for de novo identification, delineating multiple N- and O-glycosylation sites on single glycopeptides, and deriving more glycan structure information to discriminate isomeric glycoforms are well acknowledged practical problems to be tackled.
33664361	4	44	gly	N-glycosylation	701:715	arg2	both potential N-glycosylation sites			both potential N-glycosylation sites						sites	The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites.
33664361	4	12	gly	glycosylated	629:640	arg1	both potential N-glycosylation sites	protein 1		sites		Fterm		protein 1		sites	The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites.
33664361	4	12	gly	glycosylated	629:640	arg2	both potential N-glycosylation sites	protein 1		sites		Fterm		protein 1		sites	The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites.
33664361	4	12	gly	glycosylated	629:640	arg1	NS1	NS1		sites		PUBTATOR		NS1	5781	sites	The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites.
33664361	4	12	gly	glycosylated	629:640	arg2	both potential N-glycosylation sites			sites						sites	The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites.
33664361	4	12	gly	glycosylated	629:640	arg2	both potential N-glycosylation sites	NS1		sites		PUBTATOR		NS1	5781	sites	The viral non-structural protein 1 (NS1) was glycosylated exclusively with high-mannose structures on both potential N-glycosylation sites.
28614667	0	100	gly	N-Glycosylation	0:14	arg1	Asparagine 130			Asparagine 130				Human Calcitonin Receptor	799	Asparagine 130	N-Glycosylation of Asparagine 130 in the Extracellular Domain of the Human Calcitonin Receptor Significantly Increases Peptide Hormone Affinity.
31516400	5	44	gly	glycoproteins	969:981	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A database resource termed N-GlycositeAtlas was created and further used for the distribution analyses of glycoproteins among different human cells, tissues and body fluids.
33295603	0	81	gly	O-glycosylation	78:92	arg2	the clustered O-glycosylation sites	IgA1		sites		PUBTATOR		IgA1	P01876	sites	Quantitative assessment of successive carbohydrate additions to the clustered O-glycosylation sites of IgA1 by glycosyltransferases.
30535277	6	53	gly	glycoproteins	1052:1064	arg1	the isolated glycoproteins	the isolated glycoproteins				Fterm		glycoproteins			Then, N/O- glycans were released from the isolated glycoproteins with PNGase F and NaClO, and were identified by MALDI-TOF-MS, respectively.
30535277	6	82	gly	released	1025:1032	arg2	the isolated glycoproteins AND N/O- glycans	the isolated glycoproteins			N/O- glycans	Fterm		glycoproteins			Then, N/O- glycans were released from the isolated glycoproteins with PNGase F and NaClO, and were identified by MALDI-TOF-MS, respectively.
32022078	2	3	gly	O-glycopeptides	207:221	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	Mass spectrometric analysis of O-glycopeptides may reveal the identity of the peptide, the composition of the glycan and even the connection between certain sugar units, but usually only the combination of different MS/MS techniques provides sufficient information for reliable assignment.
30712865	2	41	gly	glycoprotein	341:352	arg1	The trimeric spike transmembrane glycoprotein	The trimeric spike transmembrane glycoprotein				Fterm		glycoprotein S			The trimeric spike transmembrane glycoprotein S mediates entry into host cells and is the major target of neutralizing antibodies.
30150325	7	16	gly	group	958:962	arg1	Ser947	ATX1		Ser947	Ser947		AminoAcid	ATX1	P54253	Ser947	SEC transfers an O-GlcNAc group on Ser947 of ATX1, which resides in the SET domain, thereby activating ATX1.
33116178	9	57	gly	glycoproteins	1473:1485	arg1	helminth glycoproteins	helminth glycoproteins				Fterm		glycoproteins			Furthermore, this plant expression system will fuel the development of helminth glycoproteins for pharmaceutical applications or novel anti-helminth vaccines.
34631661	10	134	gly	glycosite	1944:1952	arg2	the most abundant glycosite			the most abundant glycosite						glycosite	The N74 site represents the most abundant glycosite on both spike proteins.
30790257	2	57	gly	glycosylation	465:477	arg1	proteins	proteins				Fterm		proteins			While flippases and floppases establish membrane asymmetry, scramblases randomize the lipid distribution and play pivotal roles during blood clotting, apoptosis, and in processes such as N-linked glycosylation of proteins.
30144161	0	3	gly	Peptide	81:87	arg1	O-GlcNAcylation				O-GlcNAcylation						Distinct Effects of O-GlcNAcylation and Phosphorylation of a Tau-Derived Amyloid Peptide on Aggregation of the Native Peptide.
28369504	9	99	gly	sialoglycoproteins	1672:1689	arg1	high molecular weight O-linked sialoglycoproteins	high molecular weight O-linked sialoglycoproteins				Fterm		sialoglycoproteins			Siglec-8 and Siglec-9 ligands in airways appear to be high molecular weight O-linked sialoglycoproteins.
31471298	8	42	gly	glycans	1493:1499	arg1	ACPA-IgG	ACPA			glycans	PUBTATOR		ACPA	5657		Noteworthy, human leucocyte antigen class II shared epitope (HLA-SE) alleles associated with the presence of V-domain glycans on ACPA-IgG.
34101390	1	14	gly	glycoproteins	256:268	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Mucin-type O-glycosylation (O-glycans, O-glycome) is among the most biologically important post-translational modification in glycoproteins but O-glycan structural diversity and expression are poorly understood due to the inadequacy of current analytical methods.
31913371	3	24	gly	glycoprotein	618:629	arg1	glycoprotein biopharmaceuticals	glycoprotein biopharmaceuticals				Fterm		glycoprotein			Due to the crucial role glycans play in modulating protein function, glycosylation analysis could lead to the discovery of novel biomarkers and is of prime importance in controlling the quality of glycoprotein biopharmaceuticals.
30487799	8	44	gly	glycosite	1390:1398	arg2	the Asn-565 glycosite			the Asn-565 glycosite				Grass Carp Serum IgM	Q15327	Asn-565 glycosite	Unlike IgMs in other species, only traces of complex-type and no high-mannose glycans were found at the Asn-565 glycosite.
30633504	3	62	gly	triglycosylated	556:570	arg1	human RNase 1	human RNase 1				PUBTATOR		RNase 1	6035		By using an engineered strain of the yeast Pichia pastoris, we installed a heptasaccharide (Man5GlcNAc2) on the side chain of Asn34, Asn76, and Asn88 to produce the authentic triglycosylated form of human RNase 1.
34232571	3	85	gly	glycoproteins	342:354	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			High-throughput and comprehensive characterization of glycoproteins is crucial for structural and functional studies of altered glycosylation in biological, physiological, and pathological processes.
30355725	6	10	gly	glycosylation	865:877	arg2	individual Sog glycosylation sites			individual Sog glycosylation sites						sites	We identify conserved N-glycosylated sites and describe the effect of mutating these residues on BMP pathway activity in Drosophila Functional analysis reveals that loss of individual Sog glycosylation sites enhances BMP antagonism and/or increases the spatial range of Sog effects in the tissue.
30355725	6	32	gly	N-glycosylated	699:712	arg1	conserved N-glycosylated sites			conserved N-glycosylated sites						sites	We identify conserved N-glycosylated sites and describe the effect of mutating these residues on BMP pathway activity in Drosophila Functional analysis reveals that loss of individual Sog glycosylation sites enhances BMP antagonism and/or increases the spatial range of Sog effects in the tissue.
32109505	4	62	gly	N-glycosylation	463:477	arg1	TRPM8 channel	TRPM8 channel				PUBTATOR		TRPM8 channel	79054		This work focuses on the N-glycosylation of TRPM8 channel that was previously studied by our group in relation to proliferation and migration of tumoral cells.
29875238	14	57	gly	glycosylated	2346:2357	arg1	appropriately glycosylated α-dystroglycan	receptor			appropriately glycosylated α-dystroglycan	Fterm		receptor			Here, we demonstrate that LASV pseudovirions can utilize the PtdSer receptor TIM-1 but only in the absence of appropriately glycosylated α-dystroglycan (αDG), the high-affinity cell surface receptor for LASV.
29237830	18	21	gly	glycosylated	2756:2767	arg1	glycosylated DG	glycosylated DG				Cterm		DG	1605		In endothelial cells that express low levels of glycosylated DG, both receptors can promote LASV entry.
30940748	9	40	gly	glycosylation	1226:1238	arg1	eIF4G	eIF4G				PUBTATOR		eIF4G	1981		The differential glycosylation of eIF4A and eIF4G appears to be regulated in the initiation complex to fine-tune protein synthesis.
30940748	9	40	gly	glycosylation	1226:1238	arg1	eIF4A	eIF4A				PUBTATOR		eIF4A	1974		The differential glycosylation of eIF4A and eIF4G appears to be regulated in the initiation complex to fine-tune protein synthesis.
30479582	5	38	gly	glycopeptides	892:904	arg2	native IgG glycopeptides			native IgG glycopeptides						glycopeptides	The combined model is capable of accurately predicting the retention of native IgG glycopeptides on 2 completely different liquid chromatography-mass spectrometry systems.
29772801	6	53	gly	tails	659:663	arg1	robust O-GlcNAcylation			tails	robust O-GlcNAcylation					tails	In vitro assays with purified O-GlcNAc transferase (OGT) enzyme showed robust O-GlcNAcylation of recombinant mature lamin A tails (residues 385⁻646), with no detectable modification of lamin B1, lamin C, or 'progerin' (Δ50) tails.
34089345	0	11	gly	glycoproteins	60:72	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Tumor cells express pauci- and oligomannosidic N-glycans in glycoproteins recognized by the mannose receptor (CD206).
34089345	0	18	gly	N-glycans	47:55	arg1	glycoproteins	glycoproteins			N-glycans	Fterm		glycoproteins			Tumor cells express pauci- and oligomannosidic N-glycans in glycoproteins recognized by the mannose receptor (CD206).
32462590	5	32	gly	N-glycopeptide	1085:1098	arg2	an unbiased high confidence N-glycopeptide profile			an unbiased high confidence N-glycopeptide profile						N-glycopeptide	Here we describe a detailed analytical procedure employing hydrophilic interaction chromatography enrichment, high-resolution tandem mass spectrometry employing complementary fragmentation techniques (higher-energy collisional dissociation and electron-transfer dissociation) and a data analytics workflow to produce an unbiased high confidence N-glycopeptide profile from plant samples.
28497210	7	85	gly	glycosylation	1049:1061	arg2	the N-linked glycosylation motif			the N-linked glycosylation motif						motif	Amino acid variations in HA and NA gene resulted in changes in the N-linked glycosylation motif which may lead to immune evasion.
30593635	1	6	gly	glycoproteins	236:248	arg1	E1	envelope glycoproteins 1 and 2 (E1 and E2				PUBTATOR		envelope glycoproteins 1 and 2 (E1 and E2	6080		Posttranslational modifications (PTMs) are often required for proper folding and physiological function of proteins, including the envelope glycoproteins 1 and 2 (E1 and E2) of hepatitis C virus (HCV).
30593635	1	6	gly	glycoproteins	236:248	arg1	the envelope glycoproteins 1 and 2	envelope glycoproteins 1 and 2 (E1 and E2				PUBTATOR		envelope glycoproteins 1 and 2 (E1 and E2	6080		Posttranslational modifications (PTMs) are often required for proper folding and physiological function of proteins, including the envelope glycoproteins 1 and 2 (E1 and E2) of hepatitis C virus (HCV).
30593635	1	6	gly	glycoproteins	236:248	arg1	the envelope glycoproteins 1 and 2	envelope glycoproteins 1 and 2 (E1 and E2				PUBTATOR		envelope glycoproteins 1 and 2 (E1 and E2	6080		Posttranslational modifications (PTMs) are often required for proper folding and physiological function of proteins, including the envelope glycoproteins 1 and 2 (E1 and E2) of hepatitis C virus (HCV).
30086619	6	38	gly	glycosylation	1072:1084	arg2	the five potential N-linked glycosylation sites			the five potential N-linked glycosylation sites						sites	Finally, mutagenesis analysis revealed that among the five potential N-linked glycosylation sites, only three contributed to significant inhibition of hyaluronic activity.
30919021	9	31	gly	glycosylation	1116:1128	arg1	P4HA1	P4HA1				PUBTATOR		P4HA1	18451		These results suggest that N-linked glycosylation of P4HA1 can direct hydroxylation at specific proline residues and affect collagen maturation.
31067000	3	41	gly	N-glycosylation	358:372	arg2	The corresponding N-glycosylation sites			The corresponding N-glycosylation sites						sites	The corresponding N-glycosylation sites in ACPA V-region sequences result from somatic hypermutation, a T cell-dependent process.
31019513	6	4	gly	glycosylation	1187:1199	arg2	these glycosylation sites			these glycosylation sites						sites	We were interested in how these glycosylation sites mediate homotypic vs. heterotypic interactions.
30881698	4	28	gly	glycoprotein	656:667	arg1	Ribonuclease B	Ribonuclease B				Cterm		Ribonuclease B			Ribonuclease B was used as a model glycoprotein to compare N-glycans released by the free and immobilized enzyme.
30881698	4	28	gly	glycoprotein	656:667	arg1	a model glycoprotein	a model glycoprotein				Fterm		glycoprotein			Ribonuclease B was used as a model glycoprotein to compare N-glycans released by the free and immobilized enzyme.
29577901	8	10	gly	sites	1318:1322	arg1	the short isoform sOGT	OGT			sites	PUBTATOR		OGT	108155		We also map four new O-GlcNAc sites in the short isoform sOGT: S391, T393, S399 and S437 in the TPRs 11-13 domain.
34343291	2	42	gly	glycoproteins	332:344	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Modifications of sugar composition in glycoproteins profoundly impact the overall physiology of the organism.
30991145	5	57	gly	glycosylation	1349:1361	arg2	all four asparagines			all four asparagines						asparagines	Our results confirmed that all four asparagines are sites of N-linked glycosylation, and Western blot confirmed that mutation of each predicted N-glycosylation sited impaired glycosylation.
30991145	5	57	gly	glycosylation	1349:1361	arg2	sites			sites						sites	Our results confirmed that all four asparagines are sites of N-linked glycosylation, and Western blot confirmed that mutation of each predicted N-glycosylation sited impaired glycosylation.
34717971	0	47	gly	Glycosylation	0:12	arg1	an Expression-enhanced SARS-CoV-2 Viral Spike Mimetic	an Expression-enhanced SARS-CoV-2 Viral Spike Mimetic				OGER		Expression-enhanced SARS	P49591		Glycosylation and Serological Reactivity of an Expression-enhanced SARS-CoV-2 Viral Spike Mimetic.
33026663	0	20	gly	N-glycoforms	81:92	arg1	yeast invertase	yeast invertase				Fterm		invertase			Increased yield of enzymatic synthesis by chromatographic selection of different N-glycoforms of yeast invertase.
28232487	2	12	gly	proteins	323:330	arg1	increased O-GlcNAcylation	proteins			increased O-GlcNAcylation	Fterm		proteins			Cellular stress, including oxidative stress, results in increased O-GlcNAcylation of numerous proteins, and this increase is thought to promote cell survival.
34869209	9	84	gly	N-glycosites	1138:1149	arg2	N-glycosites			N-glycosites						N-glycosites	Those found in the HEK293-derived RBD were highly similar to those in HEK293 S1 where N-glycosites were shared.
34331854	3	54	gly	glycoproteins	548:560	arg1	N-acetylglucosamine (GlcNAc) residues	glycoproteins			N-acetylglucosamine (GlcNAc) residues	Fterm		glycoproteins			Here we describe the metabolic introduction of a diazirine photo-cross-linker onto N-acetylglucosamine (GlcNAc) residues of N-linked glycoproteins on cell surfaces.
30919021	6	19	gly	glycans	713:719	arg1	P4HA1	P4HA1			glycans	PUBTATOR		P4HA1	18451		Downregulation of Stt3b and Magt1 reduces N259 glycans on P4HA1.
33826885	3	54	gly	glycosylation	476:488	arg1	native BG505 Env	native BG505 Env				PUBTATOR		BG505 Env	100616444		To eliminate glycan holes and mimic the glycosylation of native BG505 Env, we replace all 12 NxS sequons on BG505 SOSIP with NxT.
33581334	0	78	gly	O-glycoproteins	50:64	arg1	Experimentally Characterized O-glycoproteins	Experimentally Characterized O-glycoproteins				Fterm		O-glycoproteins			OGP: A Repository of Experimentally Characterized O-glycoproteins to Facilitate Studies on O-glycosylation.
31168022	0	65	gly	glycoprotein	107:118	arg1	a 110 kDa glycoprotein	a 110 kDa glycoprotein				Fterm		glycoprotein			MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein.
27666939	7	14	gly	fucosylated	1269:1279	arg1	either primarily fucosylated or fully afucosylated glycan profiles				either primarily fucosylated or fully afucosylated glycan profiles						Here, we report generation and use of a FX knockout (FXKO) CHO host cell line that is capable of expressing antibody molecules with either primarily fucosylated or fully afucosylated glycan profiles with otherwise similar product quality attributes, depending on addition of fucose to the cell culture media.
27666939	7	57	gly	afucosylated	1290:1301	arg1	either primarily fucosylated or fully afucosylated glycan profiles				either primarily fucosylated or fully afucosylated glycan profiles						Here, we report generation and use of a FX knockout (FXKO) CHO host cell line that is capable of expressing antibody molecules with either primarily fucosylated or fully afucosylated glycan profiles with otherwise similar product quality attributes, depending on addition of fucose to the cell culture media.
33377107	1	29	gly	glycosylation	195:207	arg1	SARS-CoV-2 spike protein	SARS-CoV-2 spike protein				PUBTATOR		spike protein	43740568		This protocol describes an integrated approach for analyzing site-specific N- and O-linked glycosylation of SARS-CoV-2 spike protein by mass spectrometry.
31622635	1	50	gly	glycoprotein	123:134	arg1	Bile-salt stimulate lipase	Bile-salt stimulate lipase				PUBTATOR		Bile-salt stimulate lipase	1056		Bile-salt stimulate lipase (BSSL) is a glycoprotein found in human milk and blood that can potently bind DC-SIGN.
31622635	1	50	gly	glycoprotein	123:134	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			Bile-salt stimulate lipase (BSSL) is a glycoprotein found in human milk and blood that can potently bind DC-SIGN.
31131693	11	16	gly	kinase	1717:1722	arg1	O-GlcNAcylation	kinase A			O-GlcNAcylation	Fterm		kinase A			O-GlcNAcylation of protein kinase A catalytic subunit, a kinase for phospholamban, decreased with OGTKO.
29402915	7	58	gly	glycosylation	1340:1352	arg1	cubilin protein	cubilin protein		residues		PUBTATOR		cubilin protein	8029	residues	Quantitative mass spectrometry and mutagenesis demonstrated that N-linked glycosylation of at least 4 residues of cubilin protein was required for its surface targeting.
29671116	1	34	gly	glycoproteins	226:238	arg1	secretory glycoproteins	secretory glycoproteins				Fterm		glycoproteins			N-linked glycosylation (NLG) is a co-translational modification that is essential for the folding, stability, and trafficking of transmembrane (TM) and secretory glycoproteins.
32535395	3	89	gly	glycoproteins	487:499	arg1	uterine glycoproteins	glycoproteins			glycan composition	Fterm		glycoproteins			In this study, lectin histochemistry was used to characterise the distribution and glycan composition of uterine glycoproteins destined for secretion, and to ascertain the local effect of an embryo on glycosylation in the endometrium.
31913636	6	59	gly	glycoprotein	1242:1253	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			Rather, N-glycanation relied on a noncovalent assembly between protein and glycan, formed in the presence of the amidase, where linearization of the glycoprotein prevented this retention and subsequent N-glycanation.
30852271	3	30	gly	glycosylation	470:482	arg2	two predicted sites			two predicted sites						sites	In this study, we confirm that N-linked glycosylation occurs at two predicted sites in the M protein ectodomain of infectious bronchitis coronavirus (IBV).
31101650	8	26	gly	glycoproteins	1729:1741	arg1	some glycoproteins	some glycoproteins				Fterm		glycoproteins			These results provide new insight into CDG pathogenesis and highlight how the surface abundance of some glycoproteins can be dually impacted by abnormal glycosylation.
30289687	5	45	gly	mannosylated	875:886	arg1	mannosylated glycoproteins	mannosylated glycoproteins				Fterm		glycoproteins			Of particular interest are the FimH and PapG fimbriae, which bind to mannosylated glycoproteins and glycolipids of the galabiose series, respectively.
30289687	5	141	gly	glycoproteins	888:900	arg1	mannosylated glycoproteins	mannosylated glycoproteins				Fterm		glycoproteins			Of particular interest are the FimH and PapG fimbriae, which bind to mannosylated glycoproteins and glycolipids of the galabiose series, respectively.
32965048	11	55	gly	N-glycopeptide	2108:2121	arg2	urinary intact N-glycopeptide oxonium ions			urinary intact N-glycopeptide oxonium ions						N-glycopeptide	The elution-abundance patterns of urinary intact N-glycopeptide oxonium ions (m/z 138 and 204) of the same subject were temporally stable during the day and over 6 months.
31665542	5	42	gly	N-glycosylation	797:811	arg2	Four N-glycosylation sites			Four N-glycosylation sites						sites	Four N-glycosylation sites were identified on Mpsy_1486, which exhibited different glycosylation profiles, with N94 only found in cells cultured at 30 °C.
32693290	4	17	gly	present	831:837	arg1	the activation peptide AND the two N-linked glycans			the activation peptide	the two N-linked glycans					peptide	For N9-GP, the mono GlycoPEGylation is directed toward one of the two N-linked glycans present at Asn157 and Asn167 in the activation peptide in a one to one ratio.
32693290	4	17	gly	present	831:837	arg2	Asn167 AND the two N-linked glycans			Asn157 and Asn167	the two N-linked glycans			factor IX	P00740	Asn157 and Asn167	For N9-GP, the mono GlycoPEGylation is directed toward one of the two N-linked glycans present at Asn157 and Asn167 in the activation peptide in a one to one ratio.
31266804	9	0	gly	N-glycosylation	1750:1764	arg2	the two TRPC6 N-glycosylation sites			the two TRPC6 N-glycosylation sites						sites	However, mutating the two TRPC6 N-glycosylation sites abrogated the cytotoxicity of mutant TRPC6 and reduced its surface expression.
34089345	9	68	gly	glycoproteins	1367:1379	arg1	All enriched glycoproteins	All enriched glycoproteins				Fterm		glycoproteins			All enriched glycoproteins, of which 42 were identified, contained pauci- or oligomannose N-glycans, confirming the microarray results.
34089345	9	1	gly	contained	1411:1419	arg1	All enriched glycoproteins AND oligomannose N-glycans	All enriched glycoproteins			oligomannose N-glycans	Fterm		glycoproteins			All enriched glycoproteins, of which 42 were identified, contained pauci- or oligomannose N-glycans, confirming the microarray results.
30002126	5	4	gly	sialylation	952:962	arg1	glycan sites				glycan sites						Lectin and mass spectrometry analysis of total N-linked glycans revealed differences in high-mannose glycans, sialylation, and the occupancy of glycan sites.
31094416	3	94	gly	glycosylation	759:771	arg2	glycosylation sites			glycosylation sites						sites	Binding of Gal-3 and its carbohydrate recognition domain (CRD) to CD146 D1-D4 is greatly reduced vis-à-vis CD146 eFL, supporting the proposal of a larger number of glycosylation sites on D5.
34212152	3	15	gly	sites	750:754	arg1	N-glycans			sites	N-glycans					sites	As predicted by an analysis of the accessibility of the N-glycans for their processing enzymes derived from the MD simulations, N-glycans at different glycosylation sites showed different kinetic properties for the processing enzymes.
34212152	3	25	gly	glycosylation	736:748	arg2	different glycosylation sites			different glycosylation sites						sites	As predicted by an analysis of the accessibility of the N-glycans for their processing enzymes derived from the MD simulations, N-glycans at different glycosylation sites showed different kinetic properties for the processing enzymes.
34192331	7	5	gly	IgGs	1041:1044	arg1	multibranched N-glycans	IgGs			multibranched N-glycans	Cterm		IgGs			In this study, IgGs with multibranched N-glycans on the Fc region were prepared by using a combination of the glycosynthase/oxazoline substrate-based N-glycan remodeling technique and successive reactions with glycosyltransferases.
34192331	7	51	gly	N-glycans	1065:1073	arg1	the Fc region			the Fc region	the Fc region		Site			region	In this study, IgGs with multibranched N-glycans on the Fc region were prepared by using a combination of the glycosynthase/oxazoline substrate-based N-glycan remodeling technique and successive reactions with glycosyltransferases.
30392906	6	33	gly	glycosylation	705:717	arg2	a unique glycosylation site			a unique glycosylation site						site	Unexpectedly, we found Asn50 is a unique glycosylation site and the glycosylation mediates its interaction with ConA.
30392906	6	33	gly	glycosylation	705:717	arg2	Asn50			Asn50				Myelin protein zero-like protein 1	9019	Asn50	Unexpectedly, we found Asn50 is a unique glycosylation site and the glycosylation mediates its interaction with ConA.
28511131	9	3	gly	glycosylation	1676:1688	arg2	a glycosylation site			a glycosylation site						site	Another novel GP5 amino acid mutation (44N→44D) was found in a single subgroup 1 Henan isolate (HeNan-A9) in a glycosylation site that is known to be crucial for PRRSV infectivity.
31879931	3	69	gly	O-glycopeptides	472:486	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	Various chemical and chemoenzymatic methods have been reported so far for synthesizing different N- and O-glycopeptides.
28490633	1	29	gly	glycoproteins	242:254	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) mediates quality control of glycoproteins in the endoplasmic reticulum by attaching glucose to N-linked glycan of misfolded proteins.
28490633	1	44	gly	proteins	338:345	arg1	N-linked glycan	proteins			N-linked glycan	Fterm		proteins			The enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) mediates quality control of glycoproteins in the endoplasmic reticulum by attaching glucose to N-linked glycan of misfolded proteins.
28490633	1	51	gly	glycoprotein	174:185	arg1	glycoprotein glucosyltransferase				glycoprotein glucosyltransferase						The enzyme UDP-glucose:glycoprotein glucosyltransferase (UGGT) mediates quality control of glycoproteins in the endoplasmic reticulum by attaching glucose to N-linked glycan of misfolded proteins.
31266872	5	84	gly	glycosylation	1018:1030	arg2	a conserved N-linked glycosylation site			a conserved N-linked glycosylation site						site	Whereas a substitution in a conserved N-linked glycosylation site (N171Q) eliminates sensitivity to ADCC, a lysine-to-serine substitution in this region (K180S) increases ADCC and renders the virus susceptible to neutralization.
32915358	4	81	gly	N-deglycosylation	843:859	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Since most derivatization techniques are notoriously time-consuming, some commercial analytical kits have been developed to speed up N-deglycosylation and N-glycan labelling of glycoproteins of pharmaceutical and biological interest such as monoclonal antibodies (mAbs).
32915358	4	43	gly	glycoproteins	887:899	arg1	N-glycan labelling	glycoproteins			N-glycan labelling	Fterm		glycoproteins			Since most derivatization techniques are notoriously time-consuming, some commercial analytical kits have been developed to speed up N-deglycosylation and N-glycan labelling of glycoproteins of pharmaceutical and biological interest such as monoclonal antibodies (mAbs).
29609090	1	25	gly	glycosylates	265:276	arg1	polypeptides			polypeptides						polypeptides	N-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides.
29609090	1	58	gly	sequon	308:313	arg1	hexose monosaccharides			sequon	hexose monosaccharides					sequon	N-Glycosyltransferase (NGT) is an inverting glycosyltransferase for an unusual pathway of N-linked protein glycosylation and glycosylates polypeptides in the consensus sequon (N-(X≠P)-T/S) with hexose monosaccharides.
31600726	6	68	gly	glycoforms	890:899	arg1	FSH glycoforms	FSH glycoforms				PUBTATOR		FSH	14308		However, the direct effects of FSH glycoforms on the mouse ovarian follicle have not yet been determined.
34232571	9	33	gly	glycoproteins	1133:1145	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Basic Protocol 1: Enzymatic digestion of glycoproteins using C4-tips Basic Protocol 2: Intact glycopeptide analysis using C18/MAX-tips Basic Protocol 3: Glycan and glycosite analysis.
34232571	9	43	gly	glycopeptide	1186:1197	arg2	Intact glycopeptide analysis			Intact glycopeptide analysis						glycopeptide	Basic Protocol 1: Enzymatic digestion of glycoproteins using C4-tips Basic Protocol 2: Intact glycopeptide analysis using C18/MAX-tips Basic Protocol 3: Glycan and glycosite analysis.
34232571	9	53	gly	glycosite	1256:1264	arg2	glycosite			glycosite						glycosite	Basic Protocol 1: Enzymatic digestion of glycoproteins using C4-tips Basic Protocol 2: Intact glycopeptide analysis using C18/MAX-tips Basic Protocol 3: Glycan and glycosite analysis.
31826991	12	50	gly	glycosites	1698:1707	arg2	some glycosites			some glycosites						glycosites	Our results also demonstrate that glycan subtype can be predicted at some glycosites based on sequence comparisons and three-dimensional structural analysis.IMPORTANCE Low-pathogenicity avian influenza virus (LPAIV) subtypes can reassort with circulating human strains and pandemic viruses can emerge in human populations, as was seen in the 1957 pandemic, in which an H2 virus reassorted with the circulating H1N1 to create a novel H2N2 genotype.
28017896	3	104	gly	proteins	558:565	arg1	O-linked β-N-acetylglucosamine	proteins			O-linked β-N-acetylglucosamine	Fterm		proteins			O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational regulatory modification of intracellular proteins and governs the function of numerous proteins, both cytosolic and nuclear.
30735356	1	7	gly	glycoproteins	216:228	arg1	the viral envelope glycoproteins	the viral envelope glycoproteins				Fterm		glycoproteins			Mucin-like regions, characterized by a local high density of O-linked glycosylation, are found on the viral envelope glycoproteins of many viruses.
31616924	5	79	gly	sites	876:880	arg1	several sites			several sites						sites	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Thr18	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296		PUBTATOR		APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Thr289	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296		PUBTATOR		APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	several sites	APOE		sites		PUBTATOR		APOE	348	sites	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296		PUBTATOR		APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser197	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296		PUBTATOR		APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser296	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296		PUBTATOR		APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Thr289	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser197	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser296	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser197	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser296	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser197	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser296	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	3	gly	O-glycosylated	850:863	arg2	Ser296	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Ser296	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Thr18	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Thr289	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Thr18	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Thr289	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Thr289	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
31616924	5	79	gly	sites	876:880	arg1	Ser290	APOE		Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296				APOE	348	Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296	APOE is O-glycosylated at several sites: Thr8, Thr18, Thr194, Ser197, Thr289, Ser290 and Ser296.
32168410	8	40	gly	deglycosylated	968:981	arg1	deglycosylated and native FXIII-B	deglycosylated and native FXIII-B				PUBTATOR		FXIII-B	2165		The clearance of deglycosylated and native FXIII-B from plasma was compared in FXIII-B knock out mice.
31306420	11	83	gly	glycosylated	1686:1697	arg1	NYF	NYF, SYP				PUBTATOR		NYF, SYP	6855		Glycosylated NYT exhibited similar avian virulence properties as NYS, but resulted in higher mosquito oral infectivity than glycosylated NYS and nonglycosylated, NYP, NYF, SYP and KYS mutants.
32172531	7	63	gly	nonglycosylated	1085:1099	arg1	nonglycosylated hERG channels	nonglycosylated hERG channels				PUBTATOR		hERG channels	2078		Compared with normal glycosylated channels, nonglycosylated hERG channels were also more susceptible to cleavage mediated by CAPN, which was present in the medium of human embryonic kidney cells under normal culture conditions.
32172531	7	74	gly	glycosylated	1062:1073	arg1	normal glycosylated channels	normal glycosylated channels				Fterm		channels			Compared with normal glycosylated channels, nonglycosylated hERG channels were also more susceptible to cleavage mediated by CAPN, which was present in the medium of human embryonic kidney cells under normal culture conditions.
31266804	8	38	gly	-glycoprotein	1411:1423	arg1	the mannosyl (α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase	the mannosyl (α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase				PUBTATOR		mannosyl (α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase	4245		Deletion of the mannosyl (α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase (MGAT1), necessary for the generation of complex N-linked glycans, abrogated TRPC6 gain-of-function variant-mediated Ca2+ influx and extracellular signal-regulated kinase activation in HEK cells, but failed to diminish cytotoxicity in cultured podocytes.
29672582	8	131	gly	fucosylated	1381:1391	arg1	fucosylated N-glycan (F1 N-glycan) concentrations				fucosylated N-glycan (F1 N-glycan) concentrations						RESULTS Significant increases of IgG4 G0 N-glycan and IgG4 fucosylated N-glycan (F1 N-glycan) concentrations were observed in IgG4RD compared with healthy controls.
30479582	4	1	gly	glycopeptides	777:789	arg1	rabbit IgGs	IgGs		glycopeptides		Cterm		IgGs		glycopeptides	Here, we have devised a unified model to predict the retention behavior of glycopeptides from human IgGs and applied this to the analysis of glycopeptides from rabbit IgGs.
30479582	4	45	gly	glycopeptides	711:723	arg2	glycopeptides	IgGs		glycopeptides		Cterm		IgGs		glycopeptides	Here, we have devised a unified model to predict the retention behavior of glycopeptides from human IgGs and applied this to the analysis of glycopeptides from rabbit IgGs.
33462887	1	2	gly	glycosylated	180:191	arg1	glycosylated proteins	glycosylated proteins				Fterm		proteins			The immune scavenger protein DC-SIGN interacts with glycosylated proteins and has a putative role in facilitating viral infection.
31527085	4	48	gly	O-GlcNAcylation	505:519	arg1	ncOGT	ncOGT			O-GlcNAcylation	Cterm		ncOGT	8473		Moreover, O-GlcNAcylation of Ser389 in ncOGT (1036 amino acids) affects its nuclear translocation in HeLa cells.
31527085	4	53	gly	Ser389	524:529	arg1	O-GlcNAcylation	ncOGT		Ser389	O-GlcNAcylation			ncOGT	8473	Ser389	Moreover, O-GlcNAcylation of Ser389 in ncOGT (1036 amino acids) affects its nuclear translocation in HeLa cells.
34631661	4	28	gly	protein	1077:1083	arg1	glycans	S protein			glycans	OGER		S protein	Q15517		To further evaluate the sialic acid linkages presenting on S protein, a two-step amidation process, employing dimethylamine and ammonium hydroxide reactions in a solid support system, was developed to differentially modify the sialic acid linkages on the glycans and glycopeptides from the S protein.
34631661	4	55	gly	linkages	1024:1031	arg1	glycopeptides			glycopeptides	glycopeptides		Site			glycopeptides	To further evaluate the sialic acid linkages presenting on S protein, a two-step amidation process, employing dimethylamine and ammonium hydroxide reactions in a solid support system, was developed to differentially modify the sialic acid linkages on the glycans and glycopeptides from the S protein.
34631661	4	20	gly	glycopeptides	1052:1064	arg2	glycopeptides	S protein		glycopeptides		OGER		S protein	Q15517	glycopeptides	To further evaluate the sialic acid linkages presenting on S protein, a two-step amidation process, employing dimethylamine and ammonium hydroxide reactions in a solid support system, was developed to differentially modify the sialic acid linkages on the glycans and glycopeptides from the S protein.
34857845	7	30	gly	fucosylated	1437:1447	arg1	the core fucosylated glycans				the core fucosylated glycans						In addition, our results show that disease-associated changes in core fucosylation are peptide-dependent and further differ by branching of the core fucosylated glycans.
32681150	7	47	gly	glycosylation	1040:1052	arg1	membrane proteins	membrane proteins				Fterm		proteins			In addition, we discuss glycoproteomic analysis that yields the site-specific glycosylation of membrane proteins.
29263294	5	44	gly	attachment	881:890	arg1	the fatty acid transporter CD36 AND O-linked N-acetylglucosamine	the fatty acid transporter CD36			O-linked N-acetylglucosamine	OGER		CD36	P16671		The changes in glucose and fatty acid metabolism are interlinked via a Nox4-ATF4-dependent increase in the hexosamine biosynthetic pathway, which mediates the attachment of O-linked N-acetylglucosamine (O-GlcNAcylation) to the fatty acid transporter CD36 and enhances fatty acid utilization.
34200965	6	15	gly	glycopeptides	919:931	arg2	glycopeptides			glycopeptides						glycopeptides	The workflow started with an optimized procedure for the digestion of membrane proteins followed by the lectin-based isolation of glycopeptides.
27643667	7	75	gly	deglycosylation	626:640	arg1	MPO	MPO				PUBTATOR		MPO	4353		Three de-glycosylated MPOs were used to assay the influence of deglycosylation on microbicidal effect of MPO.
29577901	0	35	gly	receptor-α	33:42	arg1	O-GlcNAc site-mapping	X receptor			O-GlcNAc site-mapping	OGER		X receptor	Q9UBH6		O-GlcNAc site-mapping of liver X receptor-α and O-GlcNAc transferase.
30190529	4	34	gly	glycosylation	850:862	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Amino acid differences between the Jeryl Lynn vaccine strains (genotype A) and genotype G strains were predominantly located in known B-cell epitopes and in N-linked glycosylation sites on the HN protein.
30740857	3	5	gly	glycans	294:300	arg1	FVIII	FVIII			glycans	OGER		FVIII	P00451		CLEC4M binds to mannose-containing glycans on FVIII.
32553689	1	15	gly	glycosylation	144:156	arg1	serum IgG	serum IgG				Cterm		IgG			BACKGROUND Alternative glycosylation of serum IgG has been shown to be closely associated with colorectal cancer (CRC).
32692906	1	13	gly	O-glycosylated	253:266	arg1	the O-glycosylated death receptors	the O-glycosylated death receptors				Fterm		receptors			The TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis in cells by signaling through the O-glycosylated death receptors (DR4 and DR5), but the sensitivity to TRAIL-induced apoptosis of cells varies, and the attributes of this phenomenon are complex.
32692906	1	13	gly	O-glycosylated	253:266	arg1	DR5	DR5				PUBTATOR		DR5	8795		The TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis in cells by signaling through the O-glycosylated death receptors (DR4 and DR5), but the sensitivity to TRAIL-induced apoptosis of cells varies, and the attributes of this phenomenon are complex.
32692906	1	13	gly	O-glycosylated	253:266	arg1	DR4	DR4				PUBTATOR		DR4	3126		The TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis in cells by signaling through the O-glycosylated death receptors (DR4 and DR5), but the sensitivity to TRAIL-induced apoptosis of cells varies, and the attributes of this phenomenon are complex.
35140700	8	61	gly	IgG	1138:1140	arg1	The fine carbohydrate structures	IgG			The fine carbohydrate structures	Cterm		IgG			The fine carbohydrate structures of IgG were confirmed by matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight-mass spectrometry (MALDI-TOF-MS).
29569918	5	76	gly	unsialylated	1004:1015	arg1	both sialylated and unsialylated N-glycans				both sialylated and unsialylated N-glycans						Using tandem labeling with ST6Gal1 to analyze N-glycans in a prostate cancer array, we found striking differences in expression patterns of both sialylated and unsialylated N-glycans between cancerous and healthy samples.
29569918	5	85	gly	sialylated	989:998	arg1	both sialylated and unsialylated N-glycans				both sialylated and unsialylated N-glycans						Using tandem labeling with ST6Gal1 to analyze N-glycans in a prostate cancer array, we found striking differences in expression patterns of both sialylated and unsialylated N-glycans between cancerous and healthy samples.
33676668	8	88	gly	glycopeptides	1670:1682	arg2	glycopeptides enrichment			glycopeptides enrichment						glycopeptides	This work exhibits high selectivity, high sensitivity, good stability and operability, indicating its potential for applications of glycopeptides enrichment in post-translational modification proteomics.
29688069	2	34	gly	mannosylated	513:524	arg1	D494N				D494N						We report herein on the preparation of a Kupffer cell targeting type-I interferon, an albumin-IFNα2b fusion protein that contains highly mannosylated N-linked oligosaccharide chains, Man-HSA(D494N)-IFNα2b, attached by combining albumin fusion technology and site-directed mutagenesis.
29688069	2	34	gly	mannosylated	513:524	arg1	highly mannosylated N-linked oligosaccharide chains				highly mannosylated N-linked oligosaccharide chains						We report herein on the preparation of a Kupffer cell targeting type-I interferon, an albumin-IFNα2b fusion protein that contains highly mannosylated N-linked oligosaccharide chains, Man-HSA(D494N)-IFNα2b, attached by combining albumin fusion technology and site-directed mutagenesis.
29688069	2	59	gly	contains	497:504	arg1	an albumin-IFNα2b fusion protein AND D494N	an albumin-IFNα2b fusion protein			D494N	Fterm		protein			We report herein on the preparation of a Kupffer cell targeting type-I interferon, an albumin-IFNα2b fusion protein that contains highly mannosylated N-linked oligosaccharide chains, Man-HSA(D494N)-IFNα2b, attached by combining albumin fusion technology and site-directed mutagenesis.
29688069	2	59	gly	contains	497:504	arg1	an albumin-IFNα2b fusion protein AND highly mannosylated N-linked oligosaccharide chains	an albumin-IFNα2b fusion protein			highly mannosylated N-linked oligosaccharide chains	Fterm		protein			We report herein on the preparation of a Kupffer cell targeting type-I interferon, an albumin-IFNα2b fusion protein that contains highly mannosylated N-linked oligosaccharide chains, Man-HSA(D494N)-IFNα2b, attached by combining albumin fusion technology and site-directed mutagenesis.
34331854	5	45	gly	glycoproteins	872:884	arg1	cell-surface N-linked glycoproteins	cell-surface N-linked glycoproteins				Fterm		glycoproteins			We show that diazirine-modified GlcNAc can be used to covalently cross-link two extracellular GBPs, galectin-1 and cholera toxin subunit B, to cell-surface N-linked glycoproteins.
31561469	7	27	gly	histidine	783:791	arg1	the Zn2+-complexing histidine residues			histidine residues	the Zn2+-complexing histidine residues					histidine residues	Chemical modification of the Zn2+-complexing histidine residues within the active site by using diethylpyrocarbonate (DEPC) inactivates it.
34450331	8	75	gly	glycoprotein	1475:1486	arg1	glycoprotein structural changes	glycoprotein structural changes				Fterm		glycoprotein			Thus, LDA opens avenues to study glycoprotein structural changes in a range of other physiological and pathophysiological conditions relevant to acute and chronic diseases.
30487280	1	33	gly	glycoprotein	229:240	arg1	native-like, soluble envelope glycoprotein SOSIP trimers	native-like, soluble envelope glycoprotein SOSIP trimers				PUBTATOR		envelope glycoprotein SOSIP	100616444		In HIV-1 vaccine research, native-like, soluble envelope glycoprotein SOSIP trimers are widely used for immunizing animals.
31544308	8	77	gly	vitamin	1158:1164	arg1	glycan	vitamin B			glycan	Cterm		vitamin B			RESULTS Predicted microbial function showed lower amino acid, bioenergetics and lipid pathways, with functions related to vitamin B, glycan, xenobiotic metabolism, DNA/RNA synthesis, in cirrhotics from Turkey compared to the USA.
30850477	8	105	gly	N-glycosylation	1129:1143	arg2	an N-glycosylation site			an N-glycosylation site						site	No evidence was found for an overall selection bias against acquiring an N-glycosylation site, except for the CDR3 of the H chain.
28229220	1	59	gly	glycoprotein	142:153	arg1	PURPOSE HepaCAM	PURPOSE HepaCAM				PUBTATOR		|PURPOSE
HepaCAM|	Q14CZ8		PURPOSE HepaCAM, an N-linked glycoprotein that encodes a member of the immunoglobulin superfamily, has been reported to be a tumor suppressor gene that mediates diverse cellular bio-functions.
28229220	1	59	gly	glycoprotein	142:153	arg1	an N-linked glycoprotein	an N-linked glycoprotein				Fterm		glycoprotein			PURPOSE HepaCAM, an N-linked glycoprotein that encodes a member of the immunoglobulin superfamily, has been reported to be a tumor suppressor gene that mediates diverse cellular bio-functions.
31186110	8	73	gly	glycosylated	1320:1331	arg1	The purified glycosylated IFN α2b	The purified glycosylated IFN α2b				PUBTATOR		IFN α2b	3440		The purified glycosylated IFN α2b was biologically active, inhibiting the viral replication of HCV and HEV at 85% and 66%, respectively.
31406333	6	19	gly	glycosylation	1139:1151	arg2	the glycosylation site			the glycosylation site						site	X-ray crystallography showed that the glycosylation site at residue 245 is within a conserved epitope that overlaps the NA active site, explaining why it impacts antibody binding.
31406333	6	19	gly	glycosylation	1139:1151	arg2	residue 245			residue 245						residue	X-ray crystallography showed that the glycosylation site at residue 245 is within a conserved epitope that overlaps the NA active site, explaining why it impacts antibody binding.
30487280	6	102	gly	occupancy	1225:1233	arg2	the N241 site			the N241 site				Trimers Can	P35658	N241 site	The strongest evidence for redirection of the NAb response to neoepitopes, through the opening and closing of glycan holes, was obtained from trimer immunogen groups with the highest occupancy of the N241 site.
28411811	1	8	gly	glycosylation	214:226	arg1	nucleocytoplasmic and mitochondrial proteins	nucleocytoplasmic and mitochondrial proteins				Fterm		proteins			O-GlcNAcylation is a kind of dynamic O-linked glycosylation of nucleocytoplasmic and mitochondrial proteins.
30633504	2	31	gly	N-glycosylation	312:326	arg1	RNase 1	RNase 1				PUBTATOR		RNase 1	6035		The effect of N-glycosylation on the structure and function of RNase 1 is unknown.
31101650	6	52	gly	glycosylation	1356:1368	arg1	this convertase	this convertase				Fterm		convertase			Reduced glycosylation of PCSK5a in STT3A-null cells or cells treated with the oligosaccharyltransferase inhibitor NGI-1 corresponded with failure to rescue receptor processing, implying that alterations in the glycosylation of this convertase have functional consequences.
31336250	6	4	gly	aglycosylated	1321:1333	arg1	engineered aglycosylated Fc variants	engineered aglycosylated Fc variants				Fterm		variants			All mutations in this aglycosylated Fc variant were derived from previously identified beneficial mutations for engineered aglycosylated Fc variants as opposed to glycosylated variants, suggesting that significantly different sets of beneficial mutations are necessary to improve the effector function of aglycosylated Fc.
31336250	6	8	gly	aglycosylated	1220:1232	arg1	this aglycosylated Fc variant	this aglycosylated Fc variant				Fterm		variant			All mutations in this aglycosylated Fc variant were derived from previously identified beneficial mutations for engineered aglycosylated Fc variants as opposed to glycosylated variants, suggesting that significantly different sets of beneficial mutations are necessary to improve the effector function of aglycosylated Fc.
31336250	6	26	gly	glycosylated	1361:1372	arg1	glycosylated variants	glycosylated variants				Fterm		variants			All mutations in this aglycosylated Fc variant were derived from previously identified beneficial mutations for engineered aglycosylated Fc variants as opposed to glycosylated variants, suggesting that significantly different sets of beneficial mutations are necessary to improve the effector function of aglycosylated Fc.
31336250	6	76	gly	aglycosylated	1503:1515	arg1	aglycosylated Fc	aglycosylated Fc				Cterm		Fc			All mutations in this aglycosylated Fc variant were derived from previously identified beneficial mutations for engineered aglycosylated Fc variants as opposed to glycosylated variants, suggesting that significantly different sets of beneficial mutations are necessary to improve the effector function of aglycosylated Fc.
33826885	1	7	gly	glycosylation	184:196	arg2	a potential N-linked glycosylation site			a potential N-linked glycosylation site						site	Artificial glycan holes on recombinant Env-based vaccines occur when a potential N-linked glycosylation site (PNGS) is under-occupied, but not on their viral counterparts.
34670086	0	64	gly	Glycosylation	24:36	arg1	the SARS-CoV-2 Spike	the SARS-CoV-2 Spike				PUBTATOR		Spike	43740568		Suppression of O-Linked Glycosylation of the SARS-CoV-2 Spike by Quaternary Structural Restraints.
32109505	6	11	gly	glycosylation	765:777	arg1	TRPM8	TRPM8				PUBTATOR		TRPM8	79054		The glycosylation state of TRPM8 in cells untreated and treated with a deglycosylating agent was addressed with Terahertz (THz) spectroscopy.
31826991	1	130	gly	glycosylation	214:226	arg2	Seasonal influenza carrying key hemagglutinin (HA) head region glycosylation sites			Seasonal influenza carrying key hemagglutinin (HA) head region glycosylation sites						sites	Seasonal influenza carrying key hemagglutinin (HA) head region glycosylation sites can be removed from the lung by pulmonary surfactant protein D (SP-D).
30617158	11	22	gly	N-glycosylation	2087:2101	arg1	a specific enzyme activity	a specific enzyme activity				Fterm		enzyme			We demonstrate for the first time that N-glycosylation is required for a specific enzyme activity (Nap nitrate reductase) that is associated with reduced abundance of the NapAB glycoproteins.
30617158	11	64	gly	glycoproteins	2225:2237	arg1	the NapAB glycoproteins	the NapAB glycoproteins				Fterm		glycoproteins			We demonstrate for the first time that N-glycosylation is required for a specific enzyme activity (Nap nitrate reductase) that is associated with reduced abundance of the NapAB glycoproteins.
31337704	5	31	gly	glycoproteins	1233:1245	arg1	both immune and nonimmune glycoproteins	both immune and nonimmune glycoproteins				Fterm		glycoproteins			Using MS-based glycoproteomics, along with CRISPR/Cas9-KO cell lines, natural killer cell-killing assays, and RNA-Seq experiments, we now demonstrate that humans lacking functional MAGT1 have a selective deficiency in both immune and nonimmune glycoproteins, and we identified several critical glycosylation defects in important immune-response proteins and in the expression of genes involved in immunity, particularly CD28.
29530619	11	3	gly	modification	1835:1846	arg3	Nav1.5 AND Excessive O-GlcNAc modification	Nav1.5			Excessive O-GlcNAc modification	PUBTATOR		Nav1.5	6331		Excessive O-GlcNAc modification of Nav1.5 is a novel signaling event, which may be an underlying contributing factor for the development of the arrhythmogenesis in DH.
29530619	11	15	gly	Nav1.5	1851:1856	arg1	Excessive O-GlcNAc modification	Nav1.5			Excessive O-GlcNAc modification	PUBTATOR		Nav1.5	6331		Excessive O-GlcNAc modification of Nav1.5 is a novel signaling event, which may be an underlying contributing factor for the development of the arrhythmogenesis in DH.
30633504	1	57	gly	N-glycosylated	255:268	arg1	the archetypal enzyme RNase A. RNase 1	RNase 1		sites		PUBTATOR		RNase 1	6035	sites	Ribonuclease 1 (RNase 1) is the most prevalent human homologue of the archetypal enzyme RNase A. RNase 1 contains sequons for N-linked glycosylation at Asn34, Asn76, and Asn88 and is N-glycosylated at all three sites in vivo.
29454068	9	15	gly	modified	1119:1126	arg1	ribosomal RACK1 AND O-GlcNAc	ribosomal RACK1			O-GlcNAc	PUBTATOR		RACK1	14694		RESULTS: We found that ribosomal RACK1 was highly modified by O-GlcNAc at Ser122.
33578083	2	45	gly	glycopeptide	489:500	arg2	glycopeptide			glycopeptide						glycopeptide	Exact structural elucidation of glycans is generally beyond the capability of a single mass spectrometry experiment, so a reasonable level of identification for tandem mass spectrometry, taken by several glycopeptide software tools, is that of peptide sequence and glycan composition, meaning the number of monosaccharides of each distinct mass, e.g., HexNAc(2)Hex(5) rather than man5.
30850477	7	49	gly	N-glycosylation	909:923	arg2	N-glycosylation sites			N-glycosylation sites						sites	N-glycosylation sites are clustered around CDRs and the DE loop for both H and L chains, with similar frequencies for healthy donors and patients.
29402915	6	52	gly	glycosylation	1197:1209	arg1	cubilin	cubilin				PUBTATOR		cubilin	8029		Notably, the interaction between cubilin and amnionless was not sufficient, but amnionless-mediated glycosylation of cubilin was necessary for their surface expression.
29454068	10	52	gly	RACK1	1170:1174	arg1	O-GlcNAcylation	RACK1			O-GlcNAcylation	PUBTATOR		RACK1	14694		O-GlcNAcylation of RACK1 enhanced its protein stability, ribosome binding and interaction with PKCβII (PRKCB), leading to increased eukaryotic translation initiation factor 4E phosphorylation and translation of potent oncogenes in HCC cells.
30158294	14	21	gly	glycosylated	2296:2307	arg1	SERINC5	SERINC5				PUBTATOR		SERINC5	256987		Here we show that SERINC5 is a glycosylated protein and that N-glycosylation is important for its steady state expression.
30158294	14	21	gly	glycosylated	2296:2307	arg1	a glycosylated protein	a glycosylated protein				Fterm		protein			Here we show that SERINC5 is a glycosylated protein and that N-glycosylation is important for its steady state expression.
28528272	3	29	gly	unglycosylated	394:407	arg1	cystatin E/M	cystatin E/M				PUBTATOR		cystatin E/M	1474		In this study both glycosylated and unglycosylated forms of legumain and cystatin E/M were studied.
28528272	3	29	gly	unglycosylated	394:407	arg1	legumain	legumain				OGER		legumain	Q99538		In this study both glycosylated and unglycosylated forms of legumain and cystatin E/M were studied.
28528272	3	33	gly	glycosylated	377:388	arg1	cystatin E/M	cystatin E/M				PUBTATOR		cystatin E/M	1474		In this study both glycosylated and unglycosylated forms of legumain and cystatin E/M were studied.
28528272	3	33	gly	glycosylated	377:388	arg1	legumain	legumain				OGER		legumain	Q99538		In this study both glycosylated and unglycosylated forms of legumain and cystatin E/M were studied.
34379416	4	14	gly	glycosylated	670:681	arg1	yPDI	yPDI				Cterm		yPDI	64714		Yeast PDI (yPDI) from Saccharomyces cerevisiae is glycosylated at asparagine side chains and the knowledge of its five modified sites enables a realistic computational modeling.
34379416	4	14	gly	glycosylated	670:681	arg1	Yeast PDI	Yeast PDI				PUBTATOR		PDI	64714		Yeast PDI (yPDI) from Saccharomyces cerevisiae is glycosylated at asparagine side chains and the knowledge of its five modified sites enables a realistic computational modeling.
33135055	7	8	gly	epitopes	948:955	arg1	hACE2	hACE2			epitopes	PUBTATOR		hACE2	59272		To deduce the detailed structure of glycan epitopes on hACE2 that may be involved in viral binding, we have characterized the terminal sialic acid linkages, the presence of bisecting GlcNAc and the pattern of N-glycan fucosylation.
29793953	11	67	gly	nonglycosylated	2201:2215	arg1	nonglycosylated NTCP	nonglycosylated NTCP				PUBTATOR		NTCP	6554		We found differentiated HepaRG cells expressed nonglycosylated NTCP despite a wild-type coding sequence.
30029404	6	69	gly	glycopeptides	1210:1222	arg2	glycopeptides			glycopeptides						glycopeptides	Finally, it was applied to on-column enrichment of glycopeptides from digests of human serum followed by nano-LC-MS/MS analysis.
32168410	11	83	gly	FXIII-B	1267:1273	arg1	The total N-glycan profile	FXIII-B			The total N-glycan profile	PUBTATOR		FXIII-B	2165		The total N-glycan profile of FXIII-B featured nine individual structures; three were fucosylated and each structure contained at least one sialic acid.
29454317	2	87	gly	sialylated	452:461	arg1	two tumor-associated O-linked carbohydrates				two tumor-associated O-linked carbohydrates						NMIBC expresses two tumor-associated O-linked carbohydrates: the disaccharide (Galβ1,3GalNAc) Thomsen-Friedenreich (T) antigen, and its sialylated counterpart (Siaα2,3Galβ1,3GalNAc) sialyl-T (sT), synthesized by sialyltransferase ST3GAL1, whose roles in BCG response are unknown.
29454317	2	87	gly	sialylated	452:461	arg1	Siaα2,3Galβ1,3GalNAc				Siaα2,3Galβ1,3GalNAc						NMIBC expresses two tumor-associated O-linked carbohydrates: the disaccharide (Galβ1,3GalNAc) Thomsen-Friedenreich (T) antigen, and its sialylated counterpart (Siaα2,3Galβ1,3GalNAc) sialyl-T (sT), synthesized by sialyltransferase ST3GAL1, whose roles in BCG response are unknown.
31616924	10	43	gly	glycoforms	1708:1717	arg1	tissue-specific APOE glycoforms	tissue-specific APOE glycoforms				PUBTATOR		APOE	348		These data, although limited by sample size, suggest that there are tissue-specific APOE glycoforms.
31672932	8	51	gly	glycosylation	1104:1116	arg1	RIPK3	RIPK3				PUBTATOR		RIPK3	56532		Furthermore, glycosylation of RIPK3 by OGT is associated with reduced RIPK3 protein stability.
30853938	7	27	gly	glycosylates	971:982	arg1	cyclin D1	cyclin D1				PUBTATOR		cyclin D1	595		Finally, biochemical and cell imaging experiments in human cancer cells reveal that the O-GlcNAc transferase (OGT) binds to and glycosylates cyclin D1.
32282299	6	39	gly	N-glycosylation	1130:1144	arg1	IgA1	IgA1				PUBTATOR		IgA1	P01876		METHODS Capillary electrophoresis with laser induced fluorescent detection (CE-LIF) was used to analyze the N-glycosylation profiles of Z(IgA1) partitioned immunoglobulin A in pooled serum and saliva of 10 control subjects and 8 patients with malignant hematological diseases having cytostatic treatment induced mild oral mucosal lesions.
29784879	3	26	gly	glycoproteins	482:494	arg1	misfolded glycoproteins	misfolded glycoproteins				Fterm		glycoproteins			In mammals, mannose trimming from N-glycans is crucial for disposal of misfolded glycoproteins.
31281932	8	23	gly	proteins	1189:1196	arg1	N-linked glycans	proteins			N-linked glycans	Fterm		proteins			We demonstrated that the recombinant α2,6-sialyltransferase from H. cetorum is an excellent catalyst for modification of N-linked glycans of different therapeutic proteins.
29531238	2	40	gly	glycosylation	588:600	arg2	glycosylation sequon			glycosylation sequon						sequon	In this study, we update the number of human N-linked glycosylation sequons (NLGs), and we investigate cancer-relatedness of glycosylation-impacting somatic nonsynonymous single-nucleotide variation (nsSNV) by mapping human NLGs to cancer variation data and reporting the expected loss or gain of glycosylation sequon.
29531238	2	42	gly	glycosylation	345:357	arg2	human N-linked glycosylation sequons			human N-linked glycosylation sequons							In this study, we update the number of human N-linked glycosylation sequons (NLGs), and we investigate cancer-relatedness of glycosylation-impacting somatic nonsynonymous single-nucleotide variation (nsSNV) by mapping human NLGs to cancer variation data and reporting the expected loss or gain of glycosylation sequon.
33516683	11	17	gly	sialylated	1641:1650	arg1	Three sialylated N-glycans				Three sialylated N-glycans						Three sialylated N-glycans showed common elevation in AS independent of age.
33983715	7	9	gly	sialylated	1301:1310	arg1	sialylated N-glycans				sialylated N-glycans						Changes in the total amount of glycans, increase in the ratio of high-mannose type N-glycans, increase in fOSs, and changes in the ratio of sialylated N-glycans in response to ER stress were shown to be potential descriptors of ER stress.
31176190	5	70	gly	glycopeptides	813:825	arg2	glycopeptides			glycopeptides						glycopeptides	However, a kinetic analysis of glycan release from glycopeptides could provide information complementary to that of small-molecule substrates, especially if providing kinetic parameters that are immediately comparable.
33629527	3	71	gly	N-glycosylation	550:564	arg2	multiple N-glycosylation sites			multiple N-glycosylation sites						sites	The influenza A virus (IAV) proteins hemagglutinin (HA) and neuraminidase (NA) have multiple N-glycosylation sites, and alteration of N-glycan micro- and macroheterogeneity can have strong effects on virulence and immunogenicity.
32553552	9	59	gly	doubly-fucosylated	1427:1444	arg1	only the doubly-fucosylated trimannosylchitobiose core Hex3HexNAc2dHex2				only the doubly-fucosylated trimannosylchitobiose core Hex3HexNAc2dHex2						The two most common N-glycan compositions were the complex/hybrid Hex3HexNAc4dHex4 and the paucimannose structure that contains only the doubly-fucosylated trimannosylchitobiose core Hex3HexNAc2dHex2, each appearing in 22 occupied sites (13.8%).
31316108	1	83	gly	glycoproteins	251:263	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Glucosidase II (GluII) plays a major role in regulating post-translation modification of N-linked glycoproteins.
34662441	3	87	gly	glycoforms	570:579	arg1	PSMA	PSMA				PUBTATOR		PSMA	2346		METHODS Mass spectrometry was used to analyze the distribution of the site-specific glycoforms of PSMA in insect, human embryonic kidney, and prostate cancer cells, and in prostate tissue upon immunoaffinity enrichment.
30865517	1	44	gly	attachment	158:167	arg1	serine AND O-GlcNAc			serine and threonine residues	O-GlcNAc					serine and threonine residues	The attachment of O-linked β-N-acetylglucosamine (O-GlcNAc) to the serine and threonine residues of proteins in distinct cellular compartments is increasingly recognized as an important mechanism regulating cellular function.
30865517	1	44	gly	attachment	158:167	arg1	serine AND O-linked β-N-acetylglucosamine			serine and threonine residues	O-linked β-N-acetylglucosamine					serine and threonine residues	The attachment of O-linked β-N-acetylglucosamine (O-GlcNAc) to the serine and threonine residues of proteins in distinct cellular compartments is increasingly recognized as an important mechanism regulating cellular function.
30504678	0	36	gly	Glycosylation	102:114	arg1	Intercellular Adhesion Molecule-1	Intercellular Adhesion Molecule-1				OGER		Intercellular Adhesion Molecule-1	P05362		Asiatic Acid, Corosolic Acid, and Maslinic Acid Interfere with Intracellular Trafficking and N-Linked Glycosylation of Intercellular Adhesion Molecule-1.
29753740	10	77	gly	N-glycosylation	1531:1545	arg1	proteins	proteins				Fterm		proteins			These results suggest that 2DG reduces N-glycosylation of proteins following the increase of phosphorylation of GFAT1 and results in the inhibition of cell growth mediated by ER stress in pancreatic cancer cells.
29673624	6	14	gly	glycoform	1045:1053	arg1	complex glycoform				complex glycoform						At the highest concentration tested, DMJ reduced N-linked complex glycoform and core fucose levels by 15 and 14 fold, respectively, and increased high mannose level by 21 fold.
30683699	1	25	gly	deglycosylation	338:352	arg1	the unique Asn297 N-X-(T/S) sequon			the unique Asn297 N-X-(T/S) sequon						sequon	In therapeutic applications in which the Fc of IgG is critically important, the receptor binding and functional properties of the Fc are lost after deglycosylation or removal of the unique Asn297 N-X-(T/S) sequon.
29577901	2	57	gly	modified	315:322	arg1	LXRα AND O-linked β-N-acetyl-glucosamine	LXRα			O-linked β-N-acetyl-glucosamine	PUBTATOR		LXRα 	10062		We have previously shown that LXRα is post-translationally modified by O-linked β-N-acetyl-glucosamine (O-GlcNAc) with increased transcriptional activity.
29577901	2	57	gly	modified	315:322	arg3	LXRα AND O-GlcNAc	LXRα			O-GlcNAc	PUBTATOR		LXRα 	10062		We have previously shown that LXRα is post-translationally modified by O-linked β-N-acetyl-glucosamine (O-GlcNAc) with increased transcriptional activity.
30487389	4	61	gly	glycoprotein	519:530	arg1	Moojase	Moojase				Fterm		Moojase			Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5.80⁻6.88).
30487389	4	61	gly	glycoprotein	519:530	arg1	a glycoprotein	glycoprotein			N-linked glycans	Fterm		glycoprotein			Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5.80⁻6.88).
34774484	0	75	gly	N-glycosylation	0:14	arg1	Trop2	Trop2				PUBTATOR		Trop2	4070		N-glycosylation status of Trop2 impacts its surface density, interaction with claudin-7 and exosomal release.
29753740	5	15	gly	N-glycoproteins	905:919	arg1	total N-glycoproteins	total N-glycoproteins				Fterm		N-glycoproteins			Therefore, we assessed the amounts of total N-glycoproteins.
28748269	5	51	gly	had	695:697	arg1	BPIFB1 AND N-linked glycan	BPIFB1			N-linked glycan	PUBTATOR		BPIFB1	228801		BPIFB1 had N-linked glycan that reacted with Aleuria aurantia lectin, which caused two types of spots with a slightly different pI and molecular weight.
34885895	8	58	gly	glycosylation	1363:1375	arg2	N-Linked glycosylation sites			N-Linked glycosylation sites						sites	These results demonstrate that DeepNGlyPred is a robust computational technique to predict N-Linked glycosylation sites confined to N-X-[S/T] sequon.
30101988	1	4	gly	G1	401:402	arg1	[1			[1						G1"[1], we are afraid that some of the important messaging aspects of our paper might not have been articulated adequately to be fully understandable for a wider audience, i.e., not separation scientist	By reading the commentary of Bevelacqua and Mortazavi regarding our recently published paper titled as "The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1"[1], we are afraid that some of the important messaging aspects of our paper might not have been articulated adequately to be fully understandable for a wider audience, i.e., not separation scientists.
30101988	1	28	gly	scientists	594:603	arg1	i.e.			i.e.						G1"[1], we are afraid that some of the important messaging aspects of our paper might not have been articulated adequately to be fully understandable for a wider audience, i.e., not separation scientist	By reading the commentary of Bevelacqua and Mortazavi regarding our recently published paper titled as "The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1"[1], we are afraid that some of the important messaging aspects of our paper might not have been articulated adequately to be fully understandable for a wider audience, i.e., not separation scientists.
30101988	1	61	gly	N-glycosylation	361:375	arg1	human immunoglobulin G1			human immunoglobulin G1						G1"[1], we are afraid that some of the important messaging aspects of our paper might not have been articulated adequately to be fully understandable for a wider audience, i.e., not separation scientist	By reading the commentary of Bevelacqua and Mortazavi regarding our recently published paper titled as "The effect of simulated space radiation on the N-glycosylation of human immunoglobulin G1"[1], we are afraid that some of the important messaging aspects of our paper might not have been articulated adequately to be fully understandable for a wider audience, i.e., not separation scientists.
33983715	4	78	gly	asparagine-linked	574:590	arg1	N-glycans			asparagine	N-glycans					asparagine	This study therefore postulates that tracing the biosynthetic pathway of asparagine-linked glycans (N-glycans) would be a reporter for reflecting the state of the ER and serve as a quantitative descriptor of ER stress.
33983715	4	78	gly	asparagine-linked	574:590	arg1	asparagine-linked glycans			asparagine	asparagine-linked glycans					asparagine	This study therefore postulates that tracing the biosynthetic pathway of asparagine-linked glycans (N-glycans) would be a reporter for reflecting the state of the ER and serve as a quantitative descriptor of ER stress.
30659065	4	50	gly	N-glycosylation	852:866	arg2	a single N-glycosylation site			a single N-glycosylation site						site	Although IgGs usually have a single N-glycosylation site and are well studied, other antibody isotypes, e.g. IgA and IgM, that are the first responders in certain diseases, have two to five sites/monomer of antibody, and little is known about their N-glycosylation.
33676668	4	25	gly	glycosylated	933:944	arg1	glycosylated peptides			glycosylated peptides						peptides	The introduction of cationic cellulose provides not only a perfect surface charge for the composite but also a greater ability to enrich glycosylated peptides.
34358619	6	67	gly	fucosylated	1368:1378	arg1	high mannose, bisecting, and core and antenna fucosylated species				high mannose, bisecting, and core and antenna fucosylated species						Region-specific glycan maps were obtained with ∼120 N-glycan compositions in each region, revealing significant differences in "brain-type" glycans involving high mannose, bisecting, and core and antenna fucosylated species.
34358619	6	104	gly	compositions	1225:1236	arg1	each region			each region	each region		Site			region	Region-specific glycan maps were obtained with ∼120 N-glycan compositions in each region, revealing significant differences in "brain-type" glycans involving high mannose, bisecting, and core and antenna fucosylated species.
30683699	3	36	gly	glycosylation	696:708	arg2	the unique N-linked glycosylation site			the unique N-linked glycosylation site						site	The efficacy of sialylated Fc has generated an incentive to modify the unique N-linked glycosylation site at Asn297, either through chemical and enzymatic methods or by mutagenesis of the Fc, that disrupts the protein-Asn297 carbohydrate interface.
30683699	3	36	gly	glycosylation	696:708	arg2	Asn297			Asn297				Human IgG1	P01857	Asn297	The efficacy of sialylated Fc has generated an incentive to modify the unique N-linked glycosylation site at Asn297, either through chemical and enzymatic methods or by mutagenesis of the Fc, that disrupts the protein-Asn297 carbohydrate interface.
30683699	3	67	gly	protein-Asn297	819:832	arg1	the protein-Asn297 carbohydrate interface			Asn297	the protein-Asn297 carbohydrate interface			Human IgG1	P01857	Asn297	The efficacy of sialylated Fc has generated an incentive to modify the unique N-linked glycosylation site at Asn297, either through chemical and enzymatic methods or by mutagenesis of the Fc, that disrupts the protein-Asn297 carbohydrate interface.
30517873	3	44	gly	N-acetylglucosamine	500:518	arg1	the threonine 754 site	ULK1		the threonine 754 site	the threonine 754 site		SpecificSite	ULK1	8408	threonine 754 site	Here, we provide evidence that ULK1 is the attachment of O-linked N-acetylglucosamine (O-GlcNAcylated) on the threonine 754 site by O-linked N-acetylglucosamine transferase (OGT) upon glucose starvation.
30581149	3	95	gly	N-glycosylation	503:517	arg2	two N-glycosylation sites			two N-glycosylation sites						sites	METHODS One or two N-glycosylation sites were engineered into the N-terminus of the non-allergenic protein horse heart myoglobin (HHM) using synthetic gene technology.
30581149	3	102	gly	METHODS	484:490	arg1	two N-glycosylation sites			two N-glycosylation sites						sites	METHODS One or two N-glycosylation sites were engineered into the N-terminus of the non-allergenic protein horse heart myoglobin (HHM) using synthetic gene technology.
34495528	6	54	gly	N-glycosylated	753:766	arg1	the N-glycosylated nascent proteins	the N-glycosylated nascent proteins				Fterm		proteins			Subsequently, the N-glycosylated nascent proteins enter the folding step, in which N-glycans contribute largely to attaining the correct protein fold by recruiting the lectin-like chaperones, calnexin, and calreticulin.
30158294	0	50	gly	N-glycosylated	3:16	arg1	SERINC5	N-Glycosylated Form of SERINC5				PUBTATOR		N-Glycosylated Form of SERINC5	256987		An N-glycosylated form of SERINC5 is specifically incorporated into HIV-1 virions.
30420690	10	17	gly	factors	1469:1475	arg1	O-GlcNAcylation	factors			O-GlcNAcylation	Fterm		factors			Interfering in O-GlcNAcylation of the GLI transcription factors may be a novel target in controlling cancer progression and drug resistance of breast cancer.
28700571	3	4	gly	glycosylation	517:529	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Although Env can tolerate a high degree of mutation in five variable regions (V1-V5), and also at N-linked glycosylation sites that contribute roughly half the mass of Env, the functional sites for recognition of receptor CD4 and co-receptor CXCR4/CCR5 are conserved and essential for viral fitness.
31881804	0	34	gly	NSL3	25:28	arg1	O-GlcNAc-Modification	NSL3			O-GlcNAc-Modification	PUBTATOR		NSL3	55683		O-GlcNAc-Modification of NSL3 at Thr755 Site Maintains the Holoenzyme Activity of MOF/NSL Histone Acetyltransfease Complex.
33186014	6	80	gly	glycoprotein	1077:1088	arg1	glycoprotein	glycoprotein				Fterm		glycoprotein			Here, we screened wild-type versus mutant strains of the gastric pathogen Helicobacter pylori, ultimately permitting the identification of genes involved in glycoprotein and lipopolysaccharide biosynthesis.
34212152	5	60	gly	structures	1092:1101	arg1	the same protein	protein			structures	Fterm		protein			We propose that the observed differential N-glycan reactivities depend on the surrounding protein tertiary structure and lead to different glycan structures in the same protein through kinetically controlled processing pathways.
34864058	8	8	gly	glycoproteins	1587:1599	arg1	O-glycosylated intestinal epithelial glycoproteins	O-glycosylated intestinal epithelial glycoproteins				Fterm		glycoproteins			Moreover, we show that increased expression of B3GNT7 alone is sufficient to promote increased display of Lex-decorated carbohydrate glycan structures primarily on O-glycosylated intestinal epithelial glycoproteins.
34864058	8	53	gly	O-glycosylated	1550:1563	arg1	O-glycosylated intestinal epithelial glycoproteins	O-glycosylated intestinal epithelial glycoproteins				Fterm		glycoproteins			Moreover, we show that increased expression of B3GNT7 alone is sufficient to promote increased display of Lex-decorated carbohydrate glycan structures primarily on O-glycosylated intestinal epithelial glycoproteins.
31918287	5	25	gly	glycoprotein	661:672	arg1	glycoprotein class	glycoprotein class				Fterm		glycoprotein			METHODS The distribution, molecular size and glycoprotein class of Siglec-E ligands on mouse aorta were determined, and the protein carrier of the ligands was identified.
31306674	9	65	gly	glycosylated	1458:1469	arg1	some key glycosylated host immune receptors	some key glycosylated host immune receptors				Fterm		receptors	3458		This work demonstrates that iminosugar treatment of primary macrophages affects expression and functionality of some key glycosylated host immune receptors important in the dengue life cycle.
34780171	5	46	gly	glycopeptides	1126:1138	arg2	glycopeptides			glycopeptides						glycopeptides	The stepwise elution shows a high glycopeptide fractionation efficiency by a <10% overlap of glycopeptides between adjacent fractions.
34780171	5	80	gly	glycopeptide	1067:1078	arg2	a high glycopeptide fractionation efficiency			a high glycopeptide fractionation efficiency						glycopeptide	The stepwise elution shows a high glycopeptide fractionation efficiency by a <10% overlap of glycopeptides between adjacent fractions.
30158294	5	61	gly	modified	626:633	arg1	virion-associated SERINC5 AND N-linked, complex glycans	virion-associated SERINC5			N-linked, complex glycans	PUBTATOR		SERINC5	256987		We used various glycosidases to establish that virion-associated SERINC5 is modified by N-linked, complex glycans, whereas the majority of SERINC5 in cells is of relatively low molecular weight and is modified by high-mannose glycans.
29687407	9	70	gly	glycosylation	1733:1745	arg2	putative N-linked glycosylation sites			putative N-linked glycosylation sites						sites	Translated sequences revealed potentially functional differences between brain and non-brain sequences in the location of putative N-linked glycosylation sites (N-sites), V1 length, V3 charge, and the number of V4 N-sites.
29687407	9	70	gly	glycosylation	1733:1745	arg2	N-sites			N-sites						N-sites	Translated sequences revealed potentially functional differences between brain and non-brain sequences in the location of putative N-linked glycosylation sites (N-sites), V1 length, V3 charge, and the number of V4 N-sites.
29687407	9	70	gly	glycosylation	1733:1745	arg1	V4 N-sites			N-sites						N-sites	Translated sequences revealed potentially functional differences between brain and non-brain sequences in the location of putative N-linked glycosylation sites (N-sites), V1 length, V3 charge, and the number of V4 N-sites.
29687407	9	70	gly	glycosylation	1733:1745	arg1	V4 N-sites			N-sites						N-sites	Translated sequences revealed potentially functional differences between brain and non-brain sequences in the location of putative N-linked glycosylation sites (N-sites), V1 length, V3 charge, and the number of V4 N-sites.
29899104	10	68	gly	glycosylation	1728:1740	arg1	the E protein	the E protein				Fterm		protein			The mutation S156P results in disruption of N-linked glycosylation at amino acid 154 of the E protein and changes the conformation of "150 loop" of the E protein, which induces limited virus replication in lungs and abrogates transmission between ducks.
31308178	7	45	gly	Asn-247	1459:1465	arg1	N-glycans			Asn-247	N-glycans			vascular endothelial growth factor receptor 2	3791	Asn-247	We propose that N-glycosylation, specifically the capping of N-glycans at Asn-247 by sialic acid, tunes ligand-dependent activation and signaling of VEGFR2 in endothelial cells.
30623643	9	39	gly	glycoproteins	1572:1584	arg1	These "heavy" labeled glycoproteins	These "heavy" labeled glycoproteins				Fterm		glycoproteins			These "heavy" labeled glycoproteins represent a valuable tool for probing in vivo activity of host-associated bacterial communities and their interactions with the mucosal barrier.
29780502	2	37	gly	O-mannosylation	342:356	arg1	intrinsically disordered regions			regions	intrinsically disordered regions		Site			regions	O-linked glycans in particular vary widely in extent and chemistry in eukaryotes, with secreted proteins from fungi and yeast commonly exhibiting O-mannosylation in intrinsically disordered regions of proteins, likely for proteolysis protection, among other functions.
31913636	2	50	gly	de-N-glycosylated	468:484	arg1	its de-N-glycosylated positions			its de-N-glycosylated positions						positions	We found that following pruning of N-glycan by the amidase PNGase F, the principal influenza vaccine antigen and major viral spike protein hemagglutinin (HA) spontaneously reattached N-glycan to its de-N-glycosylated positions when the amidase was removed from solution.
29304374	10	16	gly	fucosylation	1698:1709	arg1	N-glycans				N-glycans						Importantly, identification of additional affected individuals can be easily achieved through analysis of core fucosylation of N-glycans.
31012303	4	62	gly	used	830:833	arg2	9-histidine-DHP			9-histidine-DHP						histidine	Two pyrrole-amino acid conjugates, (±)-6,7-dihydro-7-hydroxy-1-hydroxymethyl-5 H-pyrrolizine (DHP)-cysteine (7-cysteine-DHP) and 9-histidine-DHP, were synthesized and used to demonstrate that acidified ethanolic AgNO3 derivatization can cleave both S-linkage and N-linkage of pyrrole-protein adducts.
31516400	2	9	gly	glycoproteins	284:296	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure and protein function.
31516400	2	80	gly	glycoprotein	413:424	arg1	glycoprotein structure	glycoprotein structure				Fterm		glycoprotein			The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure and protein function.
30980499	5	52	gly	N-glycoproteins	552:566	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			N-glycoproteins were enriched by lectin affinity chromatography and identified by mass spectrometry.
33806155	7	79	gly	O-glycosylation	1163:1177	arg2	peptides			peptides						peptides	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.
29983388	5	4	gly	glycans	932:938	arg1	a specific position			a specific position	a specific position		Site			position	Finally, we use in silico modeling to show that core fucosylations restrict the degrees of freedom of glycans on the integrin's surface, hence stabilizing glycans on a specific position.
31168022	12	69	gly	N-glycosylation	1713:1727	arg2	N-glycosylation sites			N-glycosylation sites						sites	N-glycosidase F digestion of the 110 kDa GP and desmocollin suggested that although both proteins contain N-glycosylation sites, they are not identical.
30217930	7	20	gly	glycosylation	1507:1519	arg1	NIS	NIS				PUBTATOR		NIS	6528		Moreover, PTEN or PI3K/AKT/mTOR signaling could affect DPAGT1, a glycosylating enzyme involved in the initial step of N-linked glycosylation, to inhibit glycosylation of NIS.
32298669	12	39	gly	glycosylation	2128:2140	arg1	therapeutic proteins	therapeutic proteins				Fterm		proteins			The elucidation of mechanism of determined changes requires further investigation, but the described analytical approach represent effective platform for determination, screening and evaluation of glycosylation of therapeutic proteins.
28687873	8	60	gly	α2,6-sialylated	1541:1555	arg1	α2,6-sialylated receptor	α2,6-sialylated receptor				Fterm		receptor			CONCLUSIONS The study provides evidence of optimized therapeutic strategy in oncolytic virotherapy via partly defining α2,6-sialylated receptor as a "cellular marker" for NDV.
30600443	12	38	gly	N-glycosylation	1152:1166	arg1	the CaV3.1-T-type Ca2+ channel	the CaV3.1-T-type Ca2+ channel				Fterm		channel			These findings suggest that N-glycosylation contributes not only to the cell surface expression of the CaV3.1-T-type Ca2+ channel but to the regulation of the gating properties of the channel when the channel proteins were processed during the folding and trafficking steps in the cell.
29437916	1	40	gly	glycosylation	112:124	arg1	alpha-dystroglycan				alpha-dystroglycan						BACKGROUND Defects in glycosylation of alpha-dystroglycan (α-DG) cause autosomal-recessive disorders with wide clinical and genetic heterogeneity, with phenotypes ranging from congenital muscular dystrophies to milder limb girdle muscular dystrophies.
32890705	6	2	gly	glycoproteins	1008:1020	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			RESULTS Our in vitro enzyme assays using various types of substrates including oligosaccharides and glycoproteins revealed that the V354N mutant had dramatically reduced activity for all tested substrates with an altered substrate preference and that K361A had reduced activity for an oligosaccharide with asparagine (Asn), but not a shorter oligosaccharide without the reducing end of GlcNAc and Asn.
30135544	2	26	gly	glycosylation	241:253	arg1	prion protein	prion protein				PUBTATOR		prion protein	5621		The effects of glycosylation on prion protein (PrP) structure and function have not been thoroughly elucidated to date.
30135544	2	26	gly	glycosylation	241:253	arg1	PrP	PrP				PUBTATOR		PrP	5621		The effects of glycosylation on prion protein (PrP) structure and function have not been thoroughly elucidated to date.
31622635	2	38	gly	glycosylated	268:279	arg1	O-linked glycosylated 11 amino acid repeats				O-linked glycosylated 11 amino acid repeats						The BSSL gene is highly polymorphic with a variant number of O-linked glycosylated 11 amino acid repeats at the C-terminus of the protein, encoded in exon 11 of the gene.
27728760	2	39	gly	N-glycoproteins	467:481	arg1	21 surface N-glycoproteins	21 surface N-glycoproteins				Fterm		N-glycoproteins			We report successful modification of a surface chemistry method for use with exosomes and identify 21 surface N-glycoproteins on exosomes released by mouse mammary carcinoma-induced MDSCs.
31616924	4	27	gly	glycoforms	822:831	arg1	APOE	APOE				PUBTATOR		APOE	348		Detailed LC-MS/MS analyses allowed the identification of the glycosite and the attached glycan and site occupancy for all detectable glycosites on APOE and further three-dimensional modeling of physiological glycoforms of APOE.
31616924	4	68	gly	glycosites	747:756	arg2	all detectable glycosites			all detectable glycosites						glycosites	Detailed LC-MS/MS analyses allowed the identification of the glycosite and the attached glycan and site occupancy for all detectable glycosites on APOE and further three-dimensional modeling of physiological glycoforms of APOE.
31616924	4	16	gly	glycosite	675:683	arg1	the attached glycan and site occupancy			glycosite	the attached glycan and site occupancy					glycosite	Detailed LC-MS/MS analyses allowed the identification of the glycosite and the attached glycan and site occupancy for all detectable glycosites on APOE and further three-dimensional modeling of physiological glycoforms of APOE.
31616924	4	16	gly	glycosite	675:683	arg1	the attached glycan and site occupancy			glycosite	the attached glycan and site occupancy					glycosite	Detailed LC-MS/MS analyses allowed the identification of the glycosite and the attached glycan and site occupancy for all detectable glycosites on APOE and further three-dimensional modeling of physiological glycoforms of APOE.
31616924	4	16	gly	glycosite	675:683	arg1	the attached glycan and site occupancy			glycosite	the attached glycan and site occupancy					glycosite	Detailed LC-MS/MS analyses allowed the identification of the glycosite and the attached glycan and site occupancy for all detectable glycosites on APOE and further three-dimensional modeling of physiological glycoforms of APOE.
31616924	4	73	gly	occupancy	718:726	arg2	the glycosite			glycosite						glycosite	Detailed LC-MS/MS analyses allowed the identification of the glycosite and the attached glycan and site occupancy for all detectable glycosites on APOE and further three-dimensional modeling of physiological glycoforms of APOE.
30587575	8	38	gly	kinase	1221:1226	arg1	The O-GlcNAcylation levels	kinase			The O-GlcNAcylation levels	Fterm		kinase			The O-GlcNAcylation levels of paxillin, talin, and focal adhesion kinase were down-regulated in KD cells.
30587575	8	41	gly	paxillin	1185:1192	arg1	The O-GlcNAcylation levels	paxillin			The O-GlcNAcylation levels	PUBTATOR		paxillin	5829		The O-GlcNAcylation levels of paxillin, talin, and focal adhesion kinase were down-regulated in KD cells.
30683699	6	6	gly	attached	1335:1342	arg1	the N-terminal Asn221 sequon AND complex sialylated glycans			the N-terminal Asn221 sequon	complex sialylated glycans			Human IgG1	P01857	Asn221 sequon	The IgG1 Fc fragments containing complex sialylated glycans attached to the N-terminal Asn221 sequon bound influenza virus hemagglutinin and disrupted influenza A-mediated agglutination of human erythrocytes.
30683699	6	56	gly	sialylated	1316:1325	arg1	complex sialylated glycans				complex sialylated glycans						The IgG1 Fc fragments containing complex sialylated glycans attached to the N-terminal Asn221 sequon bound influenza virus hemagglutinin and disrupted influenza A-mediated agglutination of human erythrocytes.
30683699	6	71	gly	containing	1297:1306	arg1	The IgG1 Fc fragments AND complex sialylated glycans			The IgG1 Fc fragments	complex sialylated glycans					fragments	The IgG1 Fc fragments containing complex sialylated glycans attached to the N-terminal Asn221 sequon bound influenza virus hemagglutinin and disrupted influenza A-mediated agglutination of human erythrocytes.
30991145	3	16	gly	glycosylation	571:583	arg2	viral glycosylation sites			viral glycosylation sites						sites	Previous studies have demonstrated that changes to viral glycosylation sites may affect replication and immunogenicity, but little is known about the specific contributions of G protein glycosylation to IHNV replication and pathogenicity.
31041386	3	23	gly	protein	375:381	arg1	O-Linked N-acetylglucosamine	protein			O-Linked N-acetylglucosamine	Fterm		protein			O-Linked N-acetylglucosamine (O-GlcNAc) is a reversible protein modification found on many chromatin-associated proteins.
31041386	3	67	gly	found	396:400	arg2	many chromatin-associated proteins AND O-Linked N-acetylglucosamine	many chromatin-associated proteins			O-Linked N-acetylglucosamine	Fterm		proteins			O-Linked N-acetylglucosamine (O-GlcNAc) is a reversible protein modification found on many chromatin-associated proteins.
29070692	9	75	gly	glycoproteins	1735:1747	arg1	two glycoproteins	two glycoproteins				Fterm		glycoproteins			The M segment of the virus encodes a polyprotein precursor that is cleaved into two glycoproteins, Gn and Gc.
29070692	9	75	gly	glycoproteins	1735:1747	arg1	Gc	Gc				Cterm		Gc			The M segment of the virus encodes a polyprotein precursor that is cleaved into two glycoproteins, Gn and Gc.
30952089	6	38	gly	glycosylation	944:956	arg2	the four potential N-linked glycosylation sites			the four potential N-linked glycosylation sites						sites	Glycosylation modifications of PD-1 could be observed in three of the four potential N-linked glycosylation sites.
31777161	5	67	gly	glycoproteins	569:581	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Recently, N-glycoproteomic analysis performed on glycoproteins secreted by C. reinhardtii demonstrated that the xylosyltransferase A (XTA) was responsible for the addition of the core β(1,2)-xylose.
29933399	3	60	gly	glycoprotein	303:314	arg1	Env	Env				PUBTATOR		Env	155971		The HIV-1 envelope glycoprotein (Env) is the sole viral target of bnAbs, but is also targeted by binding, non-neutralizing antibodies.
29933399	3	60	gly	glycoprotein	303:314	arg1	The HIV-1 envelope glycoprotein	The HIV-1 envelope glycoprotein				PUBTATOR		envelope glycoprotein	155971		The HIV-1 envelope glycoprotein (Env) is the sole viral target of bnAbs, but is also targeted by binding, non-neutralizing antibodies.
33347638	8	92	gly	glycoproteins	1493:1505	arg1	serum glycoproteins	serum glycoproteins				Fterm		glycoproteins			Then, serum glycoproteins preferentially adsorbed onto Jacalin-Agarose as well as biotin-ACA/and biotin-MPA/streptavidin-immobilized magnetic beads were isolated, labeled with iTRAQ, and identified using quantitative mass spectrometry.
30814666	6	38	gly	N-glycosylated	836:849	arg1	the sites			the sites						sites	O-glycosylation constituted 83% of the events identified, while 17% of the sites were N-glycosylated.
31616924	7	74	gly	glycosylated	1108:1119	arg1	Ser197	CSF		Thr194 and Ser197		OGER		CSF		Thr194 and Ser197	APOE was hinge domain glycosylated (Thr194 and Ser197) in both CSF (27.3%) and plasma (10.3%).
31616924	7	74	gly	glycosylated	1108:1119	arg1	Thr194	CSF		Thr194 and Ser197		OGER		CSF		Thr194 and Ser197	APOE was hinge domain glycosylated (Thr194 and Ser197) in both CSF (27.3%) and plasma (10.3%).
31616924	7	74	gly	glycosylated	1108:1119	arg1	Ser197	APOE		Thr194 and Ser197		PUBTATOR		APOE	348	Thr194 and Ser197	APOE was hinge domain glycosylated (Thr194 and Ser197) in both CSF (27.3%) and plasma (10.3%).
31616924	7	74	gly	glycosylated	1108:1119	arg1	Thr194	APOE		Thr194 and Ser197		PUBTATOR		APOE	348	Thr194 and Ser197	APOE was hinge domain glycosylated (Thr194 and Ser197) in both CSF (27.3%) and plasma (10.3%).
31616924	7	74	gly	glycosylated	1108:1119	arg1	Thr194			Thr194 and Ser197				apolipoprotein E	348	Thr194 and Ser197	APOE was hinge domain glycosylated (Thr194 and Ser197) in both CSF (27.3%) and plasma (10.3%).
33263330	5	72	gly	glycoproteins	866:878	arg1	target glycoproteins	target glycoproteins				Fterm		glycoproteins			Here, we demonstrate the benefits of producing target glycoproteins in the GlycoDelete human embryonic kidney 293 cell line that has been engineered to produce N-glycans as short glycan stumps comprising N-acetylglucosamine, galactose and sialic acid.
32304323	3	2	gly	glycosylation	433:445	arg2	their sites			their sites						sites	GalNAc-Ts select their sites of glycosylation based on weak and overlapping peptide sequence motifs, as well prior substrate O-GalNAc glycosylation at sites both remote (long-range) and neighboring (short-range) the acceptor.
32304323	3	76	gly	sites	552:556	arg1	prior substrate O-GalNAc glycosylation			sites	prior substrate O-GalNAc glycosylation					sites	GalNAc-Ts select their sites of glycosylation based on weak and overlapping peptide sequence motifs, as well prior substrate O-GalNAc glycosylation at sites both remote (long-range) and neighboring (short-range) the acceptor.
32304323	3	18	gly	glycosylation	535:547	arg1	sites			sites						sites	GalNAc-Ts select their sites of glycosylation based on weak and overlapping peptide sequence motifs, as well prior substrate O-GalNAc glycosylation at sites both remote (long-range) and neighboring (short-range) the acceptor.
30633504	0	49	gly	N-Glycosylation	31:45	arg1	Human Ribonuclease 1	Human Ribonuclease 1				OGER		Human Ribonuclease 1	P07998		Consequences of the Endogenous N-Glycosylation of Human Ribonuclease 1.
29717387	6	51	gly	unglycosylated	765:778	arg1	unglycosylated PPARγ	unglycosylated PPARγ				PUBTATOR		PPAR	Q07869		PPAR wild-type (WT) transfection inhibited the inflammatory activation of microglia, while the anti-inflammatory function of unglycosylated PPARγ was down-regulated.
31129958	3	28	gly	glycopeptide	560:571	arg2	glycopeptide			glycopeptide						glycopeptide	The effectiveness of these tools is best measured and improved by determining how often they would select a glycopeptide decoy as a spectral match, instead of its correct assignment; yet generating the appropriate number and type of glycopeptide decoys can be challenging.
31129958	3	35	gly	glycopeptide	435:446	arg2	a glycopeptide decoy			a glycopeptide decoy						glycopeptide	The effectiveness of these tools is best measured and improved by determining how often they would select a glycopeptide decoy as a spectral match, instead of its correct assignment; yet generating the appropriate number and type of glycopeptide decoys can be challenging.
33167210	1	26	gly	glycoprotein	229:240	arg1	the highly abundant seminal plasma glycoprotein prostate-specific antigen	the highly abundant seminal plasma glycoprotein prostate-specific antigen				Fterm		glycoprotein			An altered total seminal plasma glycosylation has been associated with male infertility, and the highly abundant seminal plasma glycoprotein prostate-specific antigen (PSA) plays an important role in fertilization.
31616924	8	18	gly	glycosylation	1263:1275	arg1	plasma peptide283-299			plasma peptide283-299						peptide283-299	CSF APOE held almost 10-fold more abundant C-terminal (Thr289, Ser290 and Ser296) glycosylation (36.8% of CSF peptide283-299 was glycosylated, 3.8% of plasma peptide283-299), with sialylated and disialylated (Neu5Acα2-3Galβ1-3(Neu5Acα2-6) GalNAcα1-) core 1 structures.
31616924	8	72	gly	disialylated	1376:1387	arg1	disialylated (Neu5Acα2-3Galβ1-3(Neu5Acα2-6) GalNAcα1-) core 1 structures				disialylated (Neu5Acα2-3Galβ1-3(Neu5Acα2-6) GalNAcα1-) core 1 structures						CSF APOE held almost 10-fold more abundant C-terminal (Thr289, Ser290 and Ser296) glycosylation (36.8% of CSF peptide283-299 was glycosylated, 3.8% of plasma peptide283-299), with sialylated and disialylated (Neu5Acα2-3Galβ1-3(Neu5Acα2-6) GalNAcα1-) core 1 structures.
31616924	8	51	gly	glycosylated	1310:1321	arg1	plasma peptide283-299			peptide283-299						peptide283-299	CSF APOE held almost 10-fold more abundant C-terminal (Thr289, Ser290 and Ser296) glycosylation (36.8% of CSF peptide283-299 was glycosylated, 3.8% of plasma peptide283-299), with sialylated and disialylated (Neu5Acα2-3Galβ1-3(Neu5Acα2-6) GalNAcα1-) core 1 structures.
30733333	3	2	gly	detected	587:594	arg2	several eukaryotic translation initiation factors AND O-GlcNAcylation	several eukaryotic translation initiation factors			O-GlcNAcylation	Fterm		factors			O-GlcNAcylation has also been detected on several eukaryotic translation initiation factors and ribosomal proteins.
30733333	3	2	gly	detected	587:594	arg2	ribosomal proteins AND O-GlcNAcylation	ribosomal proteins			O-GlcNAcylation	Fterm		proteins			O-GlcNAcylation has also been detected on several eukaryotic translation initiation factors and ribosomal proteins.
30733333	3	22	gly	O-GlcNAcylation	557:571	arg1	several eukaryotic translation initiation factors	several eukaryotic translation initiation factors				Fterm		factors			O-GlcNAcylation has also been detected on several eukaryotic translation initiation factors and ribosomal proteins.
30733333	3	22	gly	O-GlcNAcylation	557:571	arg1	ribosomal proteins	ribosomal proteins				Fterm		proteins			O-GlcNAcylation has also been detected on several eukaryotic translation initiation factors and ribosomal proteins.
29454068	16	65	gly	receptor	2285:2292	arg1	Increased O-GlcNAcylation	receptor for activated C-kinase 1			Increased O-GlcNAcylation	PUBTATOR		receptor for activated C-kinase 1	10399		Increased O-GlcNAcylation of ribosomal receptor for activated C-kinase 1 is positively correlated with tumor growth, metastasis and recurrence in patients with hepatocellular carcinoma.
31671706	6	58	gly	glycosylation	1268:1280	arg2	the five glycosylation sites	protein		sites		Fterm		protein		sites	Based on the mapping results, the unique features in glycan microheterogeneity for the five glycosylation sites of VEGFR-IgG fusion protein were compared site-specifically and further discussed to understand the functional meaning of each glycosylation pattern.
31186110	9	21	gly	glycosylated	1534:1545	arg1	glycosylated IFN α2b	glycosylated IFN α2b				PUBTATOR		IFN α2b	3440		Pharmacokinetic studies in Wistar rats revealed 1.3 fold increase in plasma half-life for glycosylated IFN α2b compared to standard IFN α2b produced by E. coli.
30487280	3	71	gly	residue	656:662	arg1	a glycan			residue 241	a glycan					residue 241	For BG505 trimers, the dominant autologous NAb epitope in rabbits involves residues that line a cavity caused by the absence of a glycan at residue 241.
30962950	4	14	gly	glycoprotein	733:744	arg1	Serum core fucosylated quiescin sulfhydryl oxidase 1	Serum core fucosylated quiescin sulfhydryl oxidase 1				PUBTATOR		sulfhydryl oxidase 1	5768		Serum core fucosylated quiescin sulfhydryl oxidase 1 (cf-QSOX1) was identified as a leading prognostic glycoprotein that significantly correlated with HCC recurrence.
30962950	4	14	gly	glycoprotein	733:744	arg1	a leading prognostic glycoprotein	a leading prognostic glycoprotein				Fterm		glycoprotein			Serum core fucosylated quiescin sulfhydryl oxidase 1 (cf-QSOX1) was identified as a leading prognostic glycoprotein that significantly correlated with HCC recurrence.
30962950	4	73	gly	fucosylated	641:651	arg1	Serum core fucosylated quiescin sulfhydryl oxidase 1	Serum core fucosylated quiescin sulfhydryl oxidase 1				PUBTATOR		sulfhydryl oxidase 1	5768		Serum core fucosylated quiescin sulfhydryl oxidase 1 (cf-QSOX1) was identified as a leading prognostic glycoprotein that significantly correlated with HCC recurrence.
30962950	4	73	gly	fucosylated	641:651	arg1	a leading prognostic glycoprotein	a leading prognostic glycoprotein				Fterm		glycoprotein			Serum core fucosylated quiescin sulfhydryl oxidase 1 (cf-QSOX1) was identified as a leading prognostic glycoprotein that significantly correlated with HCC recurrence.
30962950	4	73	gly	fucosylated	641:651	arg1	cf-QSOX1	cf-QSOX1				PUBTATOR		QSOX1	104009		Serum core fucosylated quiescin sulfhydryl oxidase 1 (cf-QSOX1) was identified as a leading prognostic glycoprotein that significantly correlated with HCC recurrence.
29512051	3	12	gly	containing	793:802	arg1	regions AND N-linked glycans			regions	N-linked glycans					regions	Combining these data with Western blot analysis enabled the identification of the putative epitopes that appeared to span regions containing N-linked glycans.
29753090	1	48	gly	glycosylated	148:159	arg1	heavily glycosylated proteins	heavily glycosylated proteins				Fterm		proteins			Mucins are heavily glycosylated proteins with high molecular mass, and are involved in various diseases including infection, inflammation, and cancer.
34857845	3	31	gly	proteins	380:387	arg1	Fucosylation	proteins			Fucosylation	Fterm		proteins			Fucosylation of proteins regulates such processes and is associated with various diseases including autoimmunity and cancer.
34857845	3	49	gly	Fucosylation	364:375	arg1	proteins	proteins				Fterm		proteins			Fucosylation of proteins regulates such processes and is associated with various diseases including autoimmunity and cancer.
31306420	1	22	gly	glycosylation	137:149	arg2	The N-linked glycosylation motif			The N-linked glycosylation motif						motif	The N-linked glycosylation motif at amino acid position 154-156 of the envelope (E) protein of West Nile virus (WNV) is linked to enhanced murine neuroinvasiveness, avian pathogenicity and vector competence.
31604106	1	7	gly	glycoprotein	185:196	arg1	a glycoprotein hormone	a glycoprotein hormone				Fterm		glycoprotein			Human chorionic gonadotropin (hCG) is a glycoprotein hormone that exists as a heterodimer comprised of an α subunit and β subunit linked with disulfide bridges.
31892091	1	24	gly	glycopeptide	290:301	arg2	glycopeptide			glycopeptide						glycopeptide	In this study is described an on-line titanium dioxide solid-phase extraction capillary electrophoresis-mass spectrometry (TiO2-SPE-CE-MS) method for the analysis of the glycopeptide glycoforms obtained from the tryptic digests of recombinant human erythropoietin (rhEPO).
32462590	3	12	gly	glycosylation	440:452	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	The identification of N-linked glycosylation sites and their structural characterization by mass spectrometry remains challenging due to their size, relative abundance, structural heterogeneity, and polarity.
30479582	1	5	gly	glycoproteins	235:247	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The prediction of the retention behavior/time would facilitate the identification and characterization of glycoproteins, particularly the analytical challenges, such as the characterization of low-abundance glycoforms.
34780171	3	9	gly	glycopeptides	743:755	arg2	intact glycopeptides			intact glycopeptides						glycopeptides	Here, we present the first implementation of zwitterionic hydrophilic interaction chromatography with the exposed choline group (ZIC-cHILIC) in StageTip for simultaneous enrichment and fractionation of intact glycopeptides.
34670086	4	6	gly	glycosylation	575:587	arg1	the native-like soluble trimeric protein	the native-like soluble trimeric protein				Fterm		protein			Here, we show that the receptor binding domain (RBD), when expressed as a monomer, exhibits O-linked glycosylation, which is not recapitulated in the native-like soluble trimeric protein.
28871230	10	117	gly	glycoproteins	2060:2072	arg1	N-linked glycans	glycoproteins			N-linked glycans	Fterm		glycoproteins			The MALDI-TOF/TOF-MS results of N-linked glycans from the serum glycoproteins isolated by PHA-E+L-magnetic particle conjugates showed that there was an overlap of 23 N-glycan peaks (e.g., m/z 1419.743, 1663.734, and 1743.581) between CHB, and CHC patients, 5 glycan peaks (e.g., m/z 1850.878, 1866.661, and 2037.750) were presented in virus-infected hepatitis patients compared with HV, 3 glycan peaks (1460.659, 2069.740, and 2174.772) were observed only in CHC patients.
31186110	5	107	gly	N-glycosylation	852:866	arg2	potential N-glycosylation site			potential N-glycosylation site						site	Glycoengineering strategy was employed to construct IFN α2b with potential N-glycosylation site to evade the drawbacks of approved recombinant human IFN α2b drugs.
34962767	8	4	gly	O-glycosites	1239:1250	arg2	two adjacent O-glycosites			two adjacent O-glycosites						O-glycosites	The enzyme does not cleave between two adjacent O-glycosites, indicating that O-glycosylated serine/threonine is not allowed at position P1.
31094416	2	30	gly	CD146	504:508	arg1	15N-labeled Gal-3	CD146			15N-labeled Gal-3	PUBTATOR		CD146	4162		Here, we used Nuclear Magnetic Resonance (NMR) 15N-Heteronuclear Single Quantum Coherence (HSQC) spectroscopy to investigate binding between 15N-labeled Gal-3 and the extracellular domain (eFL) of purified CD146 (five Ig-like ectodomains D1-D5) and a shorter, D5-deleted version of CD146 (D1-D4).
31063103	8	26	gly	glycosylation	1181:1193	arg2	The predicted N-linked glycosylation site			The predicted N-linked glycosylation site						site	The predicted N-linked glycosylation site between amino acids 6 and 8 in the V3 region was conserved in CCR5 viruses, but not in CXCR4 viruses.
32681150	4	7	gly	glycoproteins	626:638	arg1	the glycocalyx N-glycans and O-glycans	glycoproteins			the glycocalyx N-glycans and O-glycans	Fterm		glycoproteins			This protocol describes how to comprehensively characterize the glycocalyx N-glycans and O-glycans of glycoproteins, as well as intact glycolipids in parallel, using the same enriched membrane fraction.
32681150	4	22	gly	glycoproteins	626:638	arg1	glycoproteins	glycoproteins			the glycocalyx N-glycans and O-glycans	Fterm		glycoproteins			This protocol describes how to comprehensively characterize the glycocalyx N-glycans and O-glycans of glycoproteins, as well as intact glycolipids in parallel, using the same enriched membrane fraction.
32681150	4	22	gly	glycoproteins	626:638	arg1	glycoproteins	glycoproteins			the glycocalyx N-glycans and O-glycans	Fterm		glycoproteins			This protocol describes how to comprehensively characterize the glycocalyx N-glycans and O-glycans of glycoproteins, as well as intact glycolipids in parallel, using the same enriched membrane fraction.
33525794	2	22	gly	glycoproteins	526:538	arg1	diverse glycoproteins	diverse glycoproteins				Fterm		glycoproteins			Since that time, metabolic engineering has played an increasingly important role in developing and optimizing microbial cell glyco-factories for the production of diverse glycoproteins and other glycoconjugates.
28844738	3	9	gly	glycosylation	530:542	arg2	the N-linked glycosylation site			the N-linked glycosylation site						site	This domain also contains the N-linked glycosylation site conserved in other isotypes.
32172531	5	36	gly	glycosylation	827:839	arg1	hERG	hERG				PUBTATOR		hERG	2078		Furthermore, the protective effect of BeKm-1 on hERG from PK-cleavage was lost when glycosylation of hERG was inhibited.
32809822	1	15	gly	glycoproteins	187:199	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Changes in the levels and compositions of N-glycans released from serum and plasma glycoproteins have been assessed in many diseases across many large clinical sample cohorts.
32965048	2	13	gly	N-glycopeptide	571:584	arg2	the intact N-glycopeptide database search engine			the intact N-glycopeptide database search engine						N-glycopeptide	Each glycoform functions with a particular site- and structure-specific N-glycan that can be fully characterized using state-of-the-art tandem mass spectrometry (MS/MS) and the intact N-glycopeptide database search engine GPSeeker that we recently developed.
31399531	7	90	gly	glycosylation	1299:1311	arg2	a new glycosylation site			a new glycosylation site						site	Further analysis revealed Ile to Asn substitution introduces a new glycosylation site on first EGF-like domain of protein C, thereby adversely affecting interaction of activated protein C with protein S. Activated protein C-R352Q only exhibited reduced activity in sub-physiological concentrations of Na+ and Ca2+, suggesting that this residue contributes to metal ion-binding affinity of the protease, with no apparent adverse effect on its function in the presence of physiological levels of metal ions.
30581149	10	68	gly	heterogeneity	1703:1715	arg1	carbohydrate IgE epitopes			carbohydrate IgE epitopes							IgE-reactivity and inhibition experiments established a hierarchy of plant glcyoallergens (nPhl p 4 > nCyn d 1 > nPla a 2 > nJug r 2 > nCup a 1 > nCry j 1) indicating a hitherto unknown heterogeneity of carbohydrate IgE epitopes in plants which were completely represented by HHM 2.
34551980	3	45	gly	glycosylation	695:707	arg2	all possible glycosylation site variants			all possible glycosylation site variants						site	To address this challenge, we describe a combinatorial strategy termed shotgun scanning glycomutagenesis in which DNA libraries encoding all possible glycosylation site variants of a given protein are constructed and subsequently expressed in glycosylation-competent bacteria, thereby enabling rapid determination of glycosylatable sites in the protein.
32326134	1	9	gly	glycoprotein	196:207	arg1	a gel-forming glycoprotein polymer	a gel-forming glycoprotein polymer				Fterm		glycoprotein			Bovine submaxillary mucin (BSM) is a gel-forming glycoprotein polymer, and Ser/Thr-linked glycans (O-glycans) are important in regulating BSM's viscoelasticity and polymerization.
30153274	2	74	gly	glycoproteins	407:419	arg1	major O-linked glycoproteins	major O-linked glycoproteins				Fterm		glycoproteins			P. gingivalis produces outer membrane protein A (OmpA)-like proteins (OmpALPs), Pgm6 and Pgm7, as major O-linked glycoproteins, but their pathological roles in P. gingivalis infection are largely unknown.
30153274	2	74	gly	glycoproteins	407:419	arg1	outer membrane protein A (OmpA)-like proteins	outer membrane protein A (OmpA)-like proteins				Fterm		proteins			P. gingivalis produces outer membrane protein A (OmpA)-like proteins (OmpALPs), Pgm6 and Pgm7, as major O-linked glycoproteins, but their pathological roles in P. gingivalis infection are largely unknown.
31450586	10	2	gly	glycosylation	1451:1463	arg2	the glycosylation site occupancy			the glycosylation site occupancy						site	However, alterations in 55 differentially expressed glycoproteins showed no significant change at the protein abundance level, representing that the glycosylation site occupancy was changed between the non-type subtype and luminal/basal subtypes.
31450586	10	69	gly	glycoproteins	1354:1366	arg1	55 differentially expressed glycoproteins	55 differentially expressed glycoproteins				Fterm		glycoproteins			However, alterations in 55 differentially expressed glycoproteins showed no significant change at the protein abundance level, representing that the glycosylation site occupancy was changed between the non-type subtype and luminal/basal subtypes.
32764711	0	69	gly	glycoprotein	50:61	arg1	envelope glycoprotein	envelope glycoprotein				PUBTATOR		envelope glycoprotein	64006		Comprehensive N-glycosylation mapping of envelope glycoprotein from tick-borne encephalitis virus grown in human and tick cells.
34229070	0	42	gly	O-glycosylation	26:40	arg1	APP	APP				OGER		APP	P05067		Comprehensive analysis of O-glycosylation of amyloid precursor protein (APP) using targeted and multi-fragmentation MS strategy.
34229070	0	42	gly	O-glycosylation	26:40	arg1	amyloid precursor protein	amyloid precursor protein				PUBTATOR		amyloid precursor protein	351		Comprehensive analysis of O-glycosylation of amyloid precursor protein (APP) using targeted and multi-fragmentation MS strategy.
32030495	2	13	gly	N-glycopeptides	552:566	arg2	1,336 intact N-glycopeptides			1,336 intact N-glycopeptides						N-glycopeptides	With a spectrum-level false discovery rate of ≤ 1%, 1,336 intact N-glycopeptides from the combination of 301 unique peptide backbones and 169 putative N-glycan linkages (52 monosaccharide compositions) were identified; the corresponding intact N-glycoproteins and N-glycosites were 289 and 305, respectively, among which 176 N-glycosites were confirmed with GlcNAc-containing site-determining b/y fragment ion pairs.
32030495	2	26	gly	N-glycoproteins	731:745	arg1	the corresponding intact N-glycoproteins	the corresponding intact N-glycoproteins				Fterm		N-glycoproteins			With a spectrum-level false discovery rate of ≤ 1%, 1,336 intact N-glycopeptides from the combination of 301 unique peptide backbones and 169 putative N-glycan linkages (52 monosaccharide compositions) were identified; the corresponding intact N-glycoproteins and N-glycosites were 289 and 305, respectively, among which 176 N-glycosites were confirmed with GlcNAc-containing site-determining b/y fragment ion pairs.
32030495	2	32	gly	N-glycosites	812:823	arg2	176 N-glycosites			176 N-glycosites						N-glycosites	With a spectrum-level false discovery rate of ≤ 1%, 1,336 intact N-glycopeptides from the combination of 301 unique peptide backbones and 169 putative N-glycan linkages (52 monosaccharide compositions) were identified; the corresponding intact N-glycoproteins and N-glycosites were 289 and 305, respectively, among which 176 N-glycosites were confirmed with GlcNAc-containing site-determining b/y fragment ion pairs.
32030495	2	59	gly	N-glycosites	751:762	arg2	N-glycosites			N-glycosites						N-glycosites	With a spectrum-level false discovery rate of ≤ 1%, 1,336 intact N-glycopeptides from the combination of 301 unique peptide backbones and 169 putative N-glycan linkages (52 monosaccharide compositions) were identified; the corresponding intact N-glycoproteins and N-glycosites were 289 and 305, respectively, among which 176 N-glycosites were confirmed with GlcNAc-containing site-determining b/y fragment ion pairs.
32030495	2	62	gly	fragment	884:891	arg1	GlcNAc-containing site-determining b/y fragment ion pairs				GlcNAc-containing site-determining b/y fragment ion pairs						With a spectrum-level false discovery rate of ≤ 1%, 1,336 intact N-glycopeptides from the combination of 301 unique peptide backbones and 169 putative N-glycan linkages (52 monosaccharide compositions) were identified; the corresponding intact N-glycoproteins and N-glycosites were 289 and 305, respectively, among which 176 N-glycosites were confirmed with GlcNAc-containing site-determining b/y fragment ion pairs.
32030495	2	64	gly	peptide	603:609	arg1	52 monosaccharide compositions				52 monosaccharide compositions						With a spectrum-level false discovery rate of ≤ 1%, 1,336 intact N-glycopeptides from the combination of 301 unique peptide backbones and 169 putative N-glycan linkages (52 monosaccharide compositions) were identified; the corresponding intact N-glycoproteins and N-glycosites were 289 and 305, respectively, among which 176 N-glycosites were confirmed with GlcNAc-containing site-determining b/y fragment ion pairs.
32782472	5	111	gly	N-glycosylation	999:1013	arg2	the triple N-glycosylation site deficient mutants			the triple N-glycosylation site deficient mutants						site	RESULTS Two expression hosts, Aspergillus oryzae and Trichoderma reesei, were utilized to successfully express wild-types TrCel7A (WT and WT ) and the triple N-glycosylation site deficient mutants TrCel7A N45Q, N270Q, N384Q (ΔN-glyc and ΔN-glyc ).
29499461	3	15	gly	glycoprotein	651:662	arg1	glycoprotein biotherapeutics	glycoprotein biotherapeutics				Fterm		glycoprotein			Glycosylation, therefore, is considered as one of the important critical quality attributes of glycoprotein biotherapeutics, and consequently for their biosimilar counterparts.
32461612	5	6	gly	glycoprotein	819:830	arg1	Lassa virus glycoprotein	Lassa virus glycoprotein				Fterm		glycoprotein			We provide a comparison of the global glycan density of coronavirus spikes with other viral proteins including HIV-1 envelope, Lassa virus glycoprotein complex, and influenza hemagglutinin, where glycosylation plays a known role in shielding immunogenic epitopes.
33404348	4	14	gly	glycosylation	617:629	arg1	proteins	proteins				Fterm		proteins			Since glycosylation of proteins increases their structural complexity by several orders of magnitude, glycome studies resulted in highly dynamic biomarkers that can be evaluated for cancer diagnosis, prognosis, and therapy.
33554253	6	38	gly	glycosylation	984:996	arg1	rIgGs	rIgGs				Cterm		IgGs	16059		In addition, the glycosylation pattern of rIgGs differs depending on the host cell used for expression.
29902539	8	14	gly	residues	1250:1257	arg1	O- linked glycoconjugated residues			O- linked glycoconjugated residues						residues	Furthermore, the toxin exhibited its maximal hemolytic activity at pH between 8 and 9, displaying typical N- and O- linked glycoconjugated residues (galactose (1-4) N-acetylglucosamine and sialic acid (2-3) galactose in N- and/or O-glycan complexes).
30250045	0	57	gly	sialylated	40:49	arg1	mesothelioma-specific sialylated epitope			mesothelioma-specific sialylated epitope						epitope	Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1.
34888356	4	95	gly	terminus	676:683	arg1	sialic acids			terminus	sialic acids					terminus	In particular, sialic acids at the terminus of glycoconjugates are directly implicated in immune responses, cell-cell/pathogen interactions, and tumor progression.
34229070	8	40	gly	O-glycans	1401:1409	arg1	APP	APP			O-glycans	OGER		APP	P05067		Moreover, we also observed that TNF-α could upregulate the expression of APP and the truncated O-glycans on APP in HEK-293 T cell.
34229070	8	109	gly	APP	1379:1381	arg1	the truncated O-glycans	APP			the truncated O-glycans	OGER		APP	P05067		Moreover, we also observed that TNF-α could upregulate the expression of APP and the truncated O-glycans on APP in HEK-293 T cell.
30718403	7	41	gly	domain	1214:1219	arg1	at least one D1 domain glycan				at least one D1 domain glycan						CD4 polymorphisms also reduced Env-mediated cell entry of monkey SIVs, which was dependent on at least one D1 domain glycan.
33908014	3	31	gly	glycans	505:511	arg1	the Fc domain			the Fc domain	the Fc domain		Site			domain	Here we use combinations of exoglycosidases to precisely characterize glycans on the Fc domain of therapeutic antibodies and dimeric fusion proteins.
33908014	3	31	gly	glycans	505:511	arg1	dimeric fusion proteins	proteins			glycans	Fterm		proteins			Here we use combinations of exoglycosidases to precisely characterize glycans on the Fc domain of therapeutic antibodies and dimeric fusion proteins.
33577335	7	30	gly	glycoproteins	1369:1381	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			This structure-activity relationship study on glycoproteins highlights the viability of applying the de novo approach to probe the roles of N-glycans.
29491160	8	57	gly	α2-6-sialylated	1384:1398	arg1	extended α2-6-sialylated N-linked glycans				extended α2-6-sialylated N-linked glycans						Indeed, the addition of the K193T mutation to the H5 HA of a respiratory-droplet-transmissible virus dramatically improves both binding to human trachea epithelial cells and specificity for extended α2-6-sialylated N-linked glycans recognized by human influenza viruses.IMPORTANCE Infections by avian H5N1 viruses are associated with a high mortality rate in several species, including humans.
29427717	10	25	gly	glycosylation	1332:1344	arg2	two N-linked glycosylation sites			two N-linked glycosylation sites						sites	This motif can be discovered in ShTXNL1 as 14CRPC17 and comprised two N-linked glycosylation sites at 72NISA75 and 139NESD142.
34662441	5	46	gly	glycoforms	1044:1053	arg2	the N459, N476, and N638 sequons			the N459, N476, and N638 sequons							We noted an interesting spatial distribution of the glycoforms on the PSMA surface-high mannose glycans were the dominant glycoforms at the N459, N476, and N638 sequons facing the plasma membrane, while the N121, N195, and N336 sites, located at the exposed apical PSMA domain, carried primarily complex glycans.
34662441	5	64	gly	carried	1200:1206	arg1	the N121, N195, and N336 sites AND complex glycans			the N121, N195, and N336 sites	complex glycans			prostate-specific membrane antigen	2346	N121, N195, and N336 sites	We noted an interesting spatial distribution of the glycoforms on the PSMA surface-high mannose glycans were the dominant glycoforms at the N459, N476, and N638 sequons facing the plasma membrane, while the N121, N195, and N336 sites, located at the exposed apical PSMA domain, carried primarily complex glycans.
30420690	0	38	gly	factors	37:43	arg1	O-GlcNAcylation	factors			O-GlcNAcylation	Fterm		factors			O-GlcNAcylation of GLI transcription factors in hyperglycemic conditions augments Hedgehog activity.
33554253	7	36	gly	rRb-IgGs	1126:1133	arg1	O-glycans	IgGs			O-glycans	Cterm		IgGs	16059		Therefore, we analyzed the N- and O-glycans of various rRb-IgGs expressed in HEK293 cells, detecting and quantifying 13 different N-glycan and 3 different O-glycan structures.
33554253	7	36	gly	rRb-IgGs	1126:1133	arg1	N-	IgGs			N-	Cterm		IgGs	16059		Therefore, we analyzed the N- and O-glycans of various rRb-IgGs expressed in HEK293 cells, detecting and quantifying 13 different N-glycan and 3 different O-glycan structures.
31829588	6	51	gly	fucosylated	777:787	arg1	fucosylated glycans				fucosylated glycans						Shifts in the oligomannose distribution and the number of sialylated and fucosylated glycans were observed.
30633504	6	1	gly	N-Glycosylation	982:996	arg2	Asn34			Asn34				Human Ribonuclease 1	P07998	Asn34	N-Glycosylation at Asn34 generates the most active and stable glycoforms, in accord with its sequon being highly conserved among vertebrate species.
30919021	3	46	gly	glycosylation	405:417	arg2	N259	P4HA1		N259				P4HA1	18451	N259	P4HA1 has two glycosylation sites, Asn (N) 113 and N259.
30919021	3	46	gly	glycosylation	405:417	arg2	two glycosylation sites			sites, Asn						sites, Asn	P4HA1 has two glycosylation sites, Asn (N) 113 and N259.
30422384	7	70	gly	proMMP-9	1276:1283	arg1	larger oligosaccharides	MMP-9			larger oligosaccharides	OGER		MMP-9			Compared to recombinant Sf-9 proMMP-9 glycoforms, larger oligosaccharides of human neutrophil proMMP-9 increased resistance against proteolytic activation.
30422384	7	94	gly	glycoforms	1220:1229	arg1	recombinant Sf-9 proMMP-9 glycoforms	recombinant Sf-9 proMMP-9 glycoforms				OGER		MMP-9			Compared to recombinant Sf-9 proMMP-9 glycoforms, larger oligosaccharides of human neutrophil proMMP-9 increased resistance against proteolytic activation.
32890705	8	10	gly	glycoproteins	1569:1581	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			We further performed rescue experiments using GnT-V knockout HeLa cells and confirmed the importance of these residues for modifications of glycoproteins in cells.
29681862	0	50	gly	Synthase	54:61	arg1	Increased O-GlcNAcylation	Endothelial Nitric Oxide Synthase Compromises			Increased O-GlcNAcylation	PUBTATOR		Endothelial Nitric Oxide Synthase Compromises	18127		Increased O-GlcNAcylation of Endothelial Nitric Oxide Synthase Compromises the Anti-contractile Properties of Perivascular Adipose Tissue in Metabolic Syndrome.
33103998	3	36	gly	glycoforms	319:328	arg1	Spike-protein	Spike-protein				Fterm		Spike-protein			We expressed different glycoforms of the Spike-protein and ACE2 in CRISPR-Cas9 glycoengineered cells, and developed corresponding SARS-CoV-2 pseudovirus.
33103998	3	36	gly	glycoforms	319:328	arg1	ACE2	ACE2				OGER		ACE2	Q9BYF1		We expressed different glycoforms of the Spike-protein and ACE2 in CRISPR-Cas9 glycoengineered cells, and developed corresponding SARS-CoV-2 pseudovirus.
33295603	3	22	gly	glycoproteins	464:476	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Although recent technological advances have enhanced our ability to profile glycosylation of glycoproteins, limitations in the understanding of the biosynthesis of these glycan structures remain.
33295603	3	24	gly	glycosylation	447:459	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Although recent technological advances have enhanced our ability to profile glycosylation of glycoproteins, limitations in the understanding of the biosynthesis of these glycan structures remain.
33556394	10	37	gly	β1AR	1882:1885	arg1	a structural determinant	1AR			a structural determinant	PUBTATOR		1AR	153		These studies identify the β1AR N-terminus as a structural determinant of β1AR responses that can be targeted for therapeutic advantage.
34358619	4	17	gly	glycoproteins	937:949	arg1	cell surface glycoproteins	cell surface glycoproteins				Fterm		glycoproteins			In this work, we used a well-established cell surface glycomic nanoLC-Chip-Q-TOF platform developed in our laboratory to characterize the N-glycome of membrane fractions enriched in cell surface glycoproteins obtained from specific functional brain areas.
29861440	1	6	gly	threonine	179:187	arg1	O-linked N-acetylglucosamine			serine and threonine residues	O-linked N-acetylglucosamine					serine and threonine residues	O-linked N-acetylglucosamine (O-GlcNAc) is a dynamic post-translational modification of serine and threonine residues on nuclear and cytoplasmic proteins.
29861440	1	67	gly	serine	168:173	arg1	O-linked N-acetylglucosamine			serine and threonine residues	O-linked N-acetylglucosamine					serine and threonine residues	O-linked N-acetylglucosamine (O-GlcNAc) is a dynamic post-translational modification of serine and threonine residues on nuclear and cytoplasmic proteins.
29780502	3	41	gly	glycosylated	659:670	arg1	glycosylated intrinsically disordered proteins	glycosylated intrinsically disordered proteins				Fterm		proteins			However, it is not well understood why mannose is often the preferred glycan, and more generally, if the neighboring protein sequence and glycan have coevolved to protect against proteolysis in glycosylated intrinsically disordered proteins (IDPs).
34279906	5	8	gly	glycoproteins	1103:1115	arg1	intact glycoproteins	intact glycoproteins				Fterm		glycoproteins			We show how N-glycan branching, terminal fucosylation, LacNAc extensions, and N- and O-glycan occupancy (i.e., total number of glycans) can be directly characterized on intact glycoproteins with minimal sample preparation.
34279906	5	53	gly	fucosylation	968:979	arg1	intact glycoproteins	intact glycoproteins				Fterm		glycoproteins			We show how N-glycan branching, terminal fucosylation, LacNAc extensions, and N- and O-glycan occupancy (i.e., total number of glycans) can be directly characterized on intact glycoproteins with minimal sample preparation.
30538676	4	75	gly	proteins	920:927	arg1	the O-GlcNAc modification	proteins			the O-GlcNAc modification	Fterm		proteins			Here, we demonstrate that FGF23 increased the O-GlcNAc modification of proteins in HBECs.
31913371	2	5	gly	glycoproteins	308:320	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The types and abundance of glycans on glycoproteins can vary due to diseases such as cancer, inflammation, autoimmune and neurodegenerative disorders.
31913371	2	71	gly	glycans	297:303	arg1	glycoproteins	glycoproteins			glycans	Fterm		glycoproteins			The types and abundance of glycans on glycoproteins can vary due to diseases such as cancer, inflammation, autoimmune and neurodegenerative disorders.
31826991	9	37	gly	glycosite	1263:1271	arg2	glycosite			glycosite						glycosite	We found that glycosite "N165" (H3 numbering) is occupied by high-mannose glycans in H3 HA but by complex glycans in all LPAIV HAs.
33908015	6	4	gly	glycoproteins	744:756	arg1	model glycoproteins	model glycoproteins				Fterm		glycoproteins			The sample preparation for permethylated glycans derived from model glycoproteins and complex biological samples, analyzed using LC-MS/MS, is delineated.
33908015	6	11	gly	derived	725:731	arg1	model glycoproteins AND permethylated glycans	model glycoproteins			permethylated glycans	Fterm		glycoproteins			The sample preparation for permethylated glycans derived from model glycoproteins and complex biological samples, analyzed using LC-MS/MS, is delineated.
31837192	10	79	gly	glycoproteins	1794:1806	arg1	plant-produced glycoproteins	glycoproteins			galactosylated complex N-glycans	Fterm		glycoproteins			Altogether, our data demonstrate that NbBGAL1 acts on galactosylated complex N-glycans of plant-produced glycoproteins.
32965048	13	16	gly	glycopeptide	2345:2356	arg2	glycopeptide oxonium ion patterns			glycopeptide oxonium ion patterns						glycopeptide	The results implied an interesting possibility that glycopeptide oxonium ion patterns could serve as distinguishing markers between individuals and/or between physiological and pathological states.
33581334	9	21	gly	O-glycosylation	1201:1215	arg2	O-glycosylation sites			O-glycosylation sites						sites, O-glycopeptide sequences	The first version of OGP repository and the website allow users to obtain various O-glycoprotein-related information, such as protein accession Nos., O-glycosylation sites, O-glycopeptide sequences, site-specific O-glycan structures, experimental methods, and potential O-glycosylation sites.
33581334	9	30	gly	O-glycosylation	1321:1335	arg2	potential O-glycosylation sites			potential O-glycosylation sites						sites	The first version of OGP repository and the website allow users to obtain various O-glycoprotein-related information, such as protein accession Nos., O-glycosylation sites, O-glycopeptide sequences, site-specific O-glycan structures, experimental methods, and potential O-glycosylation sites.
33581334	9	31	gly	O-glycopeptide	1224:1237	arg1	site-specific O-glycan structures			sites, O-glycopeptide sequences	site-specific O-glycan structures					sites, O-glycopeptide sequences	The first version of OGP repository and the website allow users to obtain various O-glycoprotein-related information, such as protein accession Nos., O-glycosylation sites, O-glycopeptide sequences, site-specific O-glycan structures, experimental methods, and potential O-glycosylation sites.
30368301	2	58	gly	glycoprotein	296:307	arg1	hCG	hCG				OGER		hCG			hCG is a hetero-dimeric glycoprotein, specific to the human pregnancy, consisting of an α and a β subunit, so-called hCGα and hCGβ, respectively.
30368301	2	58	gly	glycoprotein	296:307	arg1	a hetero-dimeric glycoprotein	a hetero-dimeric glycoprotein				Fterm		glycoprotein			hCG is a hetero-dimeric glycoprotein, specific to the human pregnancy, consisting of an α and a β subunit, so-called hCGα and hCGβ, respectively.
30706985	4	4	gly	positioning	555:565	arg1	the sugar			position	the sugar					position	The positioning of the reporter on the sugar significantly altered its metabolic fate.
36303733	0	15	gly	Glycans	60:66	arg1	the Spike (S) Glycoprotein	Glycoprotein			Glycans	Fterm		Glycoprotein			N-Glycosylation Network Construction and Analysis to Modify Glycans on the Spike (S) Glycoprotein of SARS-CoV-2.
34962767	12	23	gly	O-glycosites	1865:1876	arg2	O-glycosites			O-glycosites						O-glycosites	In these examples, IMPa enabled both the identification of O-glycosites and the range of complex O-glycan structures at each site.
33676668	3	76	gly	N-glycopeptides	779:793	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Here, we synthesized a new type of hydrophilic composite based on electrostatic interactions, which has been proven to be effective in immobilizing cationic cellulose on graphene oxide-dopamine carriers (expressed as GO-DA-JR), for highly specific enrichment of N-glycopeptides.
31913371	1	11	gly	glycoproteins	200:212	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Glycosylation influences the structure and functionality of glycoproteins, and is regulated by genetic and environmental factors.
32553552	2	56	gly	glycoproteins	208:220	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			It has previously been established that glycoproteins in the midgut microvillar membrane of insects are targets for toxins produced by different organisms as well as plant lectins.
30770249	6	6	gly	RIPK3	1017:1021	arg1	OGT-mediated O-GlcNAcylation	RIPK3			OGT-mediated O-GlcNAcylation	OGER		RIPK3	Q9Y572		Mechanistically, OGT-mediated O-GlcNAcylation of the serine-threonine kinase RIPK3 on threonine 467 (T467) prevented RIPK3-RIPK1 hetero- and RIPK3-RIPK3 homo-interaction and inhibited downstream innate immunity and necroptosis signaling.
32946227	3	27	gly	glycopeptide	452:463	arg2	a dimeric tyrosine-rich glycopeptide system			a dimeric tyrosine-rich glycopeptide system						glycopeptide	Here we have established a dimeric tyrosine-rich glycopeptide system for probing the corresponding hydrogelating behavior under the influence of site- and stereospecific glycosylations.
34212152	2	6	gly	glycosylation	418:430	arg2	five glycosylation sites			five glycosylation sites						sites	To understand site-specific processing, we used protein disulfide isomerase (PDI), a model protein with five glycosylation sites, for molecular dynamics (MD) simulations and compared the result to a biochemical in vitro analysis with four different glycan processing enzymes.
33270232	0	0	gly	moieties	7:14	arg1	Entamoeba histolytica ubiquitin	ubiquitin			moieties	Fterm		ubiquitin			Glycan moieties in Entamoeba histolytica ubiquitin are immunodominant.
34545811	5	19	gly	glycoproteins	1004:1016	arg1	newly synthesized glycoproteins	newly synthesized glycoproteins				Fterm		glycoproteins			They functioned as distal glycosylation stations in dendrites, terminally modifying sugars either on newly synthesized glycoproteins passing through the secretory pathway or on surface glycoproteins taken up from the endocytic pathway.
34545811	5	43	gly	glycoproteins	1070:1082	arg1	surface glycoproteins	surface glycoproteins				Fterm		glycoproteins			They functioned as distal glycosylation stations in dendrites, terminally modifying sugars either on newly synthesized glycoproteins passing through the secretory pathway or on surface glycoproteins taken up from the endocytic pathway.
34545811	5	30	gly	sugars	969:974	arg1	surface glycoproteins	glycoproteins			sugars	Fterm		glycoproteins			They functioned as distal glycosylation stations in dendrites, terminally modifying sugars either on newly synthesized glycoproteins passing through the secretory pathway or on surface glycoproteins taken up from the endocytic pathway.
29409894	10	34	gly	glycoprotein	1691:1702	arg1	glycoprotein abundance	glycoprotein abundance				Fterm		glycoprotein			Taken together, these results suggest that MNS1/2-mediated mannose trimming of N-glycans is crucial in modulating glycoprotein abundance to withstand salt stress in plants.
32960038	4	9	gly	fetuin	1046:1051	arg1	complex-type sialoglycoforms	fetuin			complex-type sialoglycoforms	Fterm		fetuin			The technique enables highly resolved chromatographic separation of over 20 high-mannose glycan isomers in ribonuclease B and a diverse range of hybrid and complex-type sialoglycoforms of fetuin.
32960038	4	15	gly	isomers	954:960	arg1	ribonuclease B	ribonuclease B			isomers	Cterm		ribonuclease B			The technique enables highly resolved chromatographic separation of over 20 high-mannose glycan isomers in ribonuclease B and a diverse range of hybrid and complex-type sialoglycoforms of fetuin.
32960038	4	65	gly	sialoglycoforms	1027:1041	arg1	fetuin	fetuin				Fterm		fetuin			The technique enables highly resolved chromatographic separation of over 20 high-mannose glycan isomers in ribonuclease B and a diverse range of hybrid and complex-type sialoglycoforms of fetuin.
36303733	3	72	gly	glycans	561:567	arg1	the virus spike protein	spike protein			glycans	PUBTATOR		spike protein	43740568		Our compilation of the network tool had 26 glycosyltransferase and glucosidase enzymes and could infer the pathway of glycosylation machinery based on glycans in the virus spike protein.
31631785	4	6	gly	glycoproteins	858:870	arg1	these viral envelope glycoproteins	these viral envelope glycoproteins				Fterm		glycoproteins			While overexpression of GILT inhibited the entry mediated by envelope glycoproteins of SARS coronavirus (SARS-CoV), Ebola virus (EBOV) and Lassa fever virus (LASV), depletion of GILT enhanced the entry mediated by these viral envelope glycoproteins.
31631785	4	54	gly	glycoproteins	693:705	arg1	envelope glycoproteins	envelope glycoproteins				Fterm		glycoproteins			While overexpression of GILT inhibited the entry mediated by envelope glycoproteins of SARS coronavirus (SARS-CoV), Ebola virus (EBOV) and Lassa fever virus (LASV), depletion of GILT enhanced the entry mediated by these viral envelope glycoproteins.
29384693	8	20	gly	glycoform	1307:1315	arg1	COX-2	COX-2				PUBTATOR		COX-2	5743		This response was attenuated by N-linked glycosylation inhibition, suggesting that palmitate impacts expression of the fully activated glycoform of COX-2.
31327656	3	13	gly	glycosylated	410:421	arg1	PD-L1	PD-L1				PUBTATOR		Because PD-L1	29126		Because PD-L1 is heavily glycosylated, we developed a method to resolve this by removing the glycan moieties from cell surface antigens via enzymatic digestion, a process termed sample deglycosylation.
34106099	8	12	gly	glycoproteins	1501:1513	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In addition, both disialic and trisialic acid modified glycans were present on synaptic glycoproteins, although oxonium ion profiling revealed that trisialic units were only present on glycoproteins in the detergent soluble fraction.
34106099	8	101	gly	glycoproteins	1404:1416	arg1	synaptic glycoproteins	synaptic glycoproteins				Fterm		glycoproteins			In addition, both disialic and trisialic acid modified glycans were present on synaptic glycoproteins, although oxonium ion profiling revealed that trisialic units were only present on glycoproteins in the detergent soluble fraction.
34106099	8	108	gly	present	1384:1390	arg1	synaptic glycoproteins AND both disialic and trisialic acid modified glycans	glycoproteins			both disialic and trisialic acid modified glycans	Fterm		glycoproteins			In addition, both disialic and trisialic acid modified glycans were present on synaptic glycoproteins, although oxonium ion profiling revealed that trisialic units were only present on glycoproteins in the detergent soluble fraction.
31875397	5	12	gly	glycosylated	1020:1031	arg1	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	The detection of the oxonium ions in the HCD scans suggests glycan attachment to peptides, allowing electron-transfer/higher-energy collision dissociation (EThcD) to be performed to selectively elucidate structural information of glycosylated neuropeptides that are buried in nonglycosylated peptides.
31875397	5	26	gly	nonglycosylated	1066:1080	arg1	nonglycosylated peptides			nonglycosylated peptides						peptides	The detection of the oxonium ions in the HCD scans suggests glycan attachment to peptides, allowing electron-transfer/higher-energy collision dissociation (EThcD) to be performed to selectively elucidate structural information of glycosylated neuropeptides that are buried in nonglycosylated peptides.
31967800	1	59	gly	N-glycopeptides	324:338	arg2	intact N-glycopeptides characterization			intact N-glycopeptides characterization						N-glycopeptides	With the optional setting of multiple stepped collisional energies (NCEs), higher-energy collisional dissociation (HCD) as available on Orbitrap instruments is a widely adopted dissociation method for intact N-glycopeptides characterization, where peptide backbones and N-glycan moieties are selectively fragmented at high and low NCEs, respectively.
32960038	3	89	gly	glycoproteins	843:855	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Here, we developed a straightforward method using self-packed capillary porous graphitic carbon (PGC) columns for nanoflow LC-MS/MS analyses of native glycans released from glycoproteins.
32960038	3	108	gly	released	829:836	arg1	glycoproteins AND native glycans	glycoproteins			native glycans	Fterm		glycoproteins			Here, we developed a straightforward method using self-packed capillary porous graphitic carbon (PGC) columns for nanoflow LC-MS/MS analyses of native glycans released from glycoproteins.
31723224	5	67	gly	glycosylation	874:886	arg1	the wild-type Cys23	variant		Cys23		Fterm		variant		Cys23	Subcellular localization studies demonstrated O-linked glycosylation of the Ser23 variant, but not the wild-type Cys23, may be a post-translational mechanism which alters its localization within the Golgi apparatus.
33806155	4	20	gly	glycosylation	732:744	arg2	O-linked glycosylation sites			O-linked glycosylation sites						sites	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S-insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).
30144161	4	2	gly	motif	645:649	arg1	O-linked glycans			motif	O-linked glycans					motif	O-linked glycans and phosphate variants of the tau protein-derived VQIVYK hexapeptide motif were generated as a simplified amyloid scaffold model and demonstrate that, while self-aggregation can be attenuated by either a single glycan or a phosphate unit, only co-incubation with the O-GlcNAc variant inhibits aggregation of the native peptide.
29718541	1	4	gly	glycoproteins	151:163	arg1	Transmembrane glycoproteins	Transmembrane glycoproteins				Fterm		glycoproteins			Transmembrane glycoproteins, synthesized at the endoplasmic reticulum (ER), generally reach the Golgi apparatus in COPII-coated vesicles en route to the cell surface.
34774484	4	54	gly	N-glycosylation	587:601	arg1	Trop2	Trop2				PUBTATOR		Trop2	4070		The current study explored the significance of N-glycosylation of Trop2 by substituting specific N-glycan addition sites by site-directed mutagenesis.
34010560	3	62	gly	glycopeptide	606:617	arg2	glycopeptide identification			glycopeptide identification						glycopeptide	Recently, electron transfer/high-energy collision dissociation (EThcD) was introduced for glycopeptide identification, which offers rich structural information on glycopepides with the fragment ions from the cleavages of both the glycan and the peptide backbone.
29093093	3	107	gly	S	433:433	arg1	a major determinant	S protein			a major determinant	OGER		S protein	P04004		The S protein is a major determinant of the zoonotic potential of coronaviruses and is also the main target of the host humoral immune response.
32817316	6	32	gly	carrying	1220:1227	arg1	two sites AND complex N-glycans			two sites	complex N-glycans					sites	No N-glycosylation signals were detected in SLP-8348 and SLP-8321, but SLP-5818 was observed to have two sites carrying complex N-glycans based on a site-specific analysis and a glycomic workflow of the permethylated glycans.
30735356	7	4	gly	glycoproteins	1241:1253	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Our data suggest that the mucin-like region modulates virus-glycosaminoglycan interactions by regulating the affinity, type, and number of glycoproteins involved in the virus-glycosaminoglycan interaction.
30178920	6	28	gly	glycoproteins	1404:1416	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In addition, the monolith was successfully applied to the enrichment of N-linked glycans in human serum samples, demonstrating its great potential for the analysis of glycoproteins.
30713537	7	144	gly	sialylated	1295:1304	arg1	galactosylated (84.5 vs. 88.4%), sialylated (14.5 vs. 17.9%) and bisecting N-acetylglucosamine-containing (8.4 vs. 10.8%) IgG Fc N-linked glycans				galactosylated (84.5 vs. 88.4%), sialylated (14.5 vs. 17.9%) and bisecting N-acetylglucosamine-containing (8.4 vs. 10.8%) IgG Fc N-linked glycans						Extremely low gestational age infants (born < 28 weeks of gestation during second trimester) had reduced IgG concentrations and decreased proportions of galactosylated (84.5 vs. 88.4%), sialylated (14.5 vs. 17.9%) and bisecting N-acetylglucosamine-containing (8.4 vs. 10.8%) IgG Fc N-linked glycans as compared to preterm infants born ≥28 weeks of gestation (during third trimester) and term infants.
30713537	7	144	gly	sialylated	1295:1304	arg1	8.4 vs. 10.8%				8.4 vs. 10.8%						Extremely low gestational age infants (born < 28 weeks of gestation during second trimester) had reduced IgG concentrations and decreased proportions of galactosylated (84.5 vs. 88.4%), sialylated (14.5 vs. 17.9%) and bisecting N-acetylglucosamine-containing (8.4 vs. 10.8%) IgG Fc N-linked glycans as compared to preterm infants born ≥28 weeks of gestation (during third trimester) and term infants.
29530659	6	54	gly	glycosylation	939:951	arg1	HA2	HA2				OGER		HA2			The Assam15 virus had an additional N-linked glycosylation on HA2 (position 557) compared to Kerala14 virus.
30697857	9	30	gly	N-glycoproteins	1299:1313	arg1	lowly expressed N-glycoproteins	lowly expressed N-glycoproteins				Fterm		N-glycoproteins			That is, N-glycoproteins that are highly expressed evolve at faster rates than lowly expressed N-glycoproteins, in contrast to what is observed among intracellular proteins.
30697857	9	57	gly	N-glycoproteins	1213:1227	arg1	N-glycoproteins	N-glycoproteins				Fterm		N-glycoproteins			That is, N-glycoproteins that are highly expressed evolve at faster rates than lowly expressed N-glycoproteins, in contrast to what is observed among intracellular proteins.
33139572	7	80	gly	fucosylated	1295:1305	arg1	fucosylated glycans				fucosylated glycans						Interestingly, changes primarily attributed to sialylated and fucosylated glycans were extensively observed during PFC development.
31892091	0	51	gly	glycopeptide	12:23	arg2	glycopeptide biomarkers			glycopeptide biomarkers						glycopeptide	Analysis of glycopeptide biomarkers by on-line TiO2 solid-phase extraction capillary electrophoresis-mass spectrometry.
33116178	3	41	gly	glycoproteins	484:496	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			As an example, the human blood fluke Schistosoma mansoni produces highly fucosylated glycan structures on glycoproteins and glycolipids.
33116178	3	42	gly	structures	470:479	arg1	glycoproteins	glycoproteins			structures	Fterm		glycoproteins			As an example, the human blood fluke Schistosoma mansoni produces highly fucosylated glycan structures on glycoproteins and glycolipids.
33116178	3	63	gly	fucosylated	451:461	arg1	highly fucosylated glycan structures				highly fucosylated glycan structures						As an example, the human blood fluke Schistosoma mansoni produces highly fucosylated glycan structures on glycoproteins and glycolipids.
30850477	10	43	gly	N-glycosylation	1365:1379	arg2	35 out of 38 (92%) nongermline N-glycosylation sites			35 out of 38 (92%) nongermline N-glycosylation sites						sites	When expressed as rmAb, 35 out of 38 (92%) nongermline N-glycosylation sites became occupied.
26040437	4	22	gly	glycosylated	873:884	arg1	the recombinant HA1 proteins	the recombinant HA1 proteins				PUBTATOR		HA1 proteins	23526		Treatment of tunicamycin and peptide-N-glycosidase F (PNGase F) further revealed that the recombinant HA1 proteins produced in insect cells were indeed glycosylated with N-linked oligosaccharide side chains.
31131693	13	11	gly	subunit	1985:1991	arg1	a novel O-GlcNAcylation	subunit			a novel O-GlcNAcylation	Fterm		subunit			We identified a novel O-GlcNAcylation of protein kinase A catalytic subunit, which may regulate calcium handling and cardiac function.
33026663	7	23	gly	glycoforms	975:984	arg1	different invertase glycoforms	different invertase glycoforms				Fterm		invertase			Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β-d-fructofuranoside (MF) synthesis.
31527367	9	82	gly	glycosylation	1397:1409	arg1	Cav3.2	Cav3.2				PUBTATOR		Cav3.2	8912		It is known that increased extracellular glucose levels enhance Cav3.2 activity through asparagine (N)-linked glycosylation of Cav3.2, which might contribute to diabetic neuropathy.
30217067	4	17	gly	hydrolase	754:762	arg1	glucose transporters	hydrolase			glucose transporters	Fterm		hydrolase			To identify the potential mechanism, we assessed the expression of O-linked β-N-acetlyglucosamine transferase (OGT) and glycoside hydrolase O-GlcNAcase (OGA), as well as hypoxia-inducible factor 1-alpha (HIF1A) and glucose transporters (GLUT1, GLUT3).
30217067	4	75	gly	transferase	722:732	arg1	glucose transporters	O-linked β-N-acetlyglucosamine transferase			glucose transporters	PUBTATOR		O-linked β-N-acetlyglucosamine transferase	8473		To identify the potential mechanism, we assessed the expression of O-linked β-N-acetlyglucosamine transferase (OGT) and glycoside hydrolase O-GlcNAcase (OGA), as well as hypoxia-inducible factor 1-alpha (HIF1A) and glucose transporters (GLUT1, GLUT3).
30305605	7	61	gly	O-glycosylated	1145:1158	arg1	differentially O-glycosylated Cel	differentially O-glycosylated Cel				PUBTATOR		Cel	12613		Here, we provide data suggesting that differentially O-glycosylated Cel could negatively affect beta cell function.
29782851	2	55	gly	glycosylation	288:300	arg1	the in vitro receptor binding properties	the in vitro receptor binding properties				Fterm		receptor			Here we report the effects of glycosylation on the in vitro receptor binding properties of Cblns.
28700571	2	34	gly	glycoprotein	314:325	arg1	envelope glycoprotein	envelope glycoprotein				Fterm		glycoprotein			The envelope glycoprotein (Env) trimer on the surface of HIV is responsible for receptor binding and fusion.
28700571	2	34	gly	glycoprotein	314:325	arg1	Env	Env				Cterm		Env			The envelope glycoprotein (Env) trimer on the surface of HIV is responsible for receptor binding and fusion.
32134190	0	43	gly	glycoprotein	16:27	arg1	glycoprotein encoding mRNA	glycoprotein encoding mRNA				Fterm		glycoprotein			Transfection of glycoprotein encoding mRNA for swift evaluation of N-glycan engineering strategies.
31616924	0	86	gly	O-glycosylation	0:14	arg1	plasma apolipoprotein E	plasma apolipoprotein E				PUBTATOR		apolipoprotein E	348		O-glycosylation on cerebrospinal fluid and plasma apolipoprotein E differs in the lipid-binding domain.
30123425	3	3	gly	attachment	376:385	arg2	serine AND O-GlcNAc			serine	O-GlcNAc					serine	O-GlcNAcylation, the attachment of β-N-acetylglucosamine (O-GlcNAc) to serine or threonine residues of intracellular proteins, modulates protein functions and is implicated in cancer pathogenesis.
30123425	3	3	gly	attachment	376:385	arg2	serine AND β-N-acetylglucosamine			serine	β-N-acetylglucosamine					serine	O-GlcNAcylation, the attachment of β-N-acetylglucosamine (O-GlcNAc) to serine or threonine residues of intracellular proteins, modulates protein functions and is implicated in cancer pathogenesis.
30123425	3	3	gly	attachment	376:385	arg1	threonine AND O-GlcNAc			threonine	O-GlcNAc					threonine residues	O-GlcNAcylation, the attachment of β-N-acetylglucosamine (O-GlcNAc) to serine or threonine residues of intracellular proteins, modulates protein functions and is implicated in cancer pathogenesis.
30123425	3	3	gly	attachment	376:385	arg1	threonine AND β-N-acetylglucosamine			threonine	β-N-acetylglucosamine					threonine residues	O-GlcNAcylation, the attachment of β-N-acetylglucosamine (O-GlcNAc) to serine or threonine residues of intracellular proteins, modulates protein functions and is implicated in cancer pathogenesis.
32109505	3	75	gly	N-glycosylated	390:403	arg1	TRPM8	TRPM8				PUBTATOR		TRPM8	79054		TRPM8 is N-glycosylated, with a single site per subunit.
33377107	5	5	gly	glycoproteins	695:707	arg1	certain glycoproteins	certain glycoproteins				Fterm		glycoproteins			Combined with bioinformatics, the glycomics-informed glycoproteomics may be useful in generating 3D molecular dynamics simulations of certain glycoproteins alone or interacting with one another.
29463718	7	62	gly	glycosylation	864:876	arg2	an N-linked extracellular site			site						site	Finally, we identify aromatic interactions via π-π bonds and cation-π bonds, glycosylation at an N-linked extracellular site, a pore-loop disulfide bond, and 24 lipid binding sites.
31671706	2	76	gly	glycosylation	390:402	arg2	five N-linked glycosylation sites			five N-linked glycosylation sites						sites	The prepared fusion protein was expected to possess a total of five N-linked glycosylation sites: two sites in the VEGFR-1 region, two sites in the VEGFR-2 region, and one site in the human IgG Fc region.
34813786	4	55	gly	Gal	663:665	arg1	core 1 O-glycan	Gal			core 1 O-glycan	OGER		Gal	Q8N6F7		Interestingly, C. savignyi sialyltransferase exhibited effectively Neu5Ac transfer to core 1 O-glycan, Gal β(1,3)GalNAc, compared to orthologous human glycosyltransferase.
30591584	5	16	gly	glycosylated	943:954	arg1	O-GalNAc glycosylated proteins				O-GalNAc glycosylated proteins						Moreover, we identified O-GalNAc glycosylated proteins in the nucleus and present solid evidence for O-GalNAc glycan synthesis in this organelle.
30962950	9	83	gly	core-fucosylated	1430:1445	arg1	the core-fucosylated glycan				the core-fucosylated glycan						Furthermore, overexpression of a mutant version of QSOX1-S, which had eliminated the core-fucosylated glycan at Asn-130, showed no demonstrable effect on invasion or metastasis of HCC cells.
32946227	4	45	gly	glycopeptides	674:686	arg2	the glycopeptides			the glycopeptides						glycopeptides	Comparison of 18 glycoforms bearing monosaccharides at Tyr4 and Tyr4' shows that the glycopeptides with either α- or β-anomers exhibit contrary gelating abilities, when the glycan moieties contain axial hydroxyl groups.
32030495	4	80	gly	N-glycopeptides	1221:1235	arg2	72 cell-surface differentially expressed intact N-glycopeptides			72 cell-surface differentially expressed intact N-glycopeptides						N-glycopeptides	With the criteria of ≥ 1.5-fold change and p value < 0.05, 72 cell-surface differentially expressed intact N-glycopeptides (DEGPs) were found in MCF-7/ADR CSCs relative to MCF-7/ADR cells, where 8 and 64 were downregulated and upregulated, respectively.
31196789	6	38	gly	glycosylation	826:838	arg2	N293			N293				Lcc9		N293	Molecular weight analysis on those mutants suggested that glycosylation should have occurred on N313 and N454 whereas not on N293 in rLcc9.
31196789	6	38	gly	glycosylation	826:838	arg2	N313			N313				Lcc9		N313 and N454	Molecular weight analysis on those mutants suggested that glycosylation should have occurred on N313 and N454 whereas not on N293 in rLcc9.
29755357	6	33	gly	non-glycosylated	1066:1081	arg1	the non-glycosylated LCN2 variants	the non-glycosylated LCN2 variants				PUBTATOR		LCN2 variants	3934		Moreover, both the glycosylated and the non-glycosylated LCN2 variants are equally targeted to exosomes, demonstrating that this post-translational modification is not necessary for proper trafficking of LCN2 into these membranous extracellular vesicles.
31300553	4	47	gly	eIF4G1	868:873	arg1	the O-GlcNAcylation	eIF4G1			the O-GlcNAcylation	PUBTATOR		eIF4G1	208643		Here, using several approaches, including site-directed mutagenesis, Click O-GlcNAc labeling, immunoblotting, and immunofluorescence and EM imaging, we provide the first evidence for a relationship between the O-GlcNAcylation of eukaryotic translation initiation factor 4γ1 (eIF4G1) and carboxypeptidase E (CPE)-dependent proinsulin processing in βOGTKO mice.
31029427	4	72	gly	glycosylation	768:780	arg2	the specific glycosylation sites			the specific glycosylation sites						sites	However, further investigations are needed to identify the specific mechanism of modification and the specific glycosylation sites on FOXA1.
33560857	7	73	gly	N-glycosylation	1243:1257	arg2	between (macro) individual N-glycosylation sites			between (macro) individual N-glycosylation sites						sites	Overall, a great heterogeneity was found both within (micro) and in between (macro) individual N-glycosylation sites.
30023808	1	62	gly	glycoproteins/glycopeptides	159:185	arg2	Efficient enrichment glycoproteins/glycopeptides			Efficient enrichment glycoproteins/glycopeptides						glycoproteins/glycopeptides	Efficient enrichment glycoproteins/glycopeptides from complex biological solutions are very important in the biomedical sciences, in particular biomarker research.
30550553	8	2	gly	has	1417:1419	arg1	GLTP AND a bound GlcCer	GLTP			a bound GlcCer	PUBTATOR		GLTP	51228		Here we found that if GLTP has a bound GlcCer the association with VAP-A is weaker.
32707739	5	44	gly	N-glycosylation	927:941	arg2	the N-glycosylation site mutant GluA1N63Q			the N-glycosylation site mutant GluA1N63Q						site	In contrast to the N-glycosylation site mutant GluA1N63Q, the cell surface expression levels of GluA1N363Q and GluA2N370Q increased in a time-dependent manner.
30712568	0	48	gly	glycopeptides	143:155	arg2	glycopeptides			glycopeptides						glycopeptides	Facile fabrication of zwitterionic magnetic composites by one-step distillation-precipitation polymerization for highly specific enrichment of glycopeptides.
31623829	1	51	gly	glycoproteins	208:220	arg1	the N-linked type glycoproteins	glycoproteins			core-GlcNAc	Fterm		glycoproteins			BACKGROUND FUT8-mediated core fucosylation, which transfers a fucose residue from GDP-fucose to core-GlcNAc of the N-linked type glycoproteins, is crucial for signaling receptors function.
29258330	1	15	gly	glycoprotein	203:214	arg1	envelope surface glycoprotein	envelope surface glycoprotein				Fterm		glycoprotein			The main goal of this work was to identify molecular signatures in envelope surface glycoprotein that may be correlated with coreceptor usage by different human immunodeficiency virus (HIV)-2 strains.
31628258	2	13	gly	glycoproteins	581:593	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A novel, temperature gradient denaturing protocol was applied on amine functionalized magnetic bead partitioned glycoproteins to circumvent the otherwise prevalent precipitation issue.
30231545	6	3	gly	AKT	953:955	arg1	the glucose metabolism regulators	AKT			the glucose metabolism regulators	PUBTATOR		AKT	11651		This was accompanied by significantly increased activation levels of the glucose metabolism regulators 160 kDa AKT substrate (AS160), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2), and glycogen synthase kinase-3β (GSK3β).
30231545	6	6	gly	glycogen	1044:1051	arg1	the glucose metabolism regulators	glycogen synthase kinase-3β 			the glucose metabolism regulators	PUBTATOR		glycogen synthase kinase-3β 	56637		This was accompanied by significantly increased activation levels of the glucose metabolism regulators 160 kDa AKT substrate (AS160), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2), and glycogen synthase kinase-3β (GSK3β).
30735356	2	28	gly	glycoprotein	333:344	arg1	its glycoprotein gC	its glycoprotein gC				Fterm		glycoprotein			Herpes simplex virus type 1 (HSV-1), for example, exhibits a mucin-like region on its glycoprotein gC, a viral protein involved in initial recruitment of the virus to the cell surface via interaction with sulfated glycosaminoglycans.
29263266	2	57	gly	lectin	393:398	arg1	GalNAc-binding affinity	lectin			GalNAc-binding affinity	PUBTATOR		lectin	547726		Here, we demonstrate that a soybean-derived lectin (SBL) with GalNAc-binding affinity could potently suppress HIV infection of macrophages in a dose-dependent fashion.
31526925	6	23	gly	sites	809:813	arg1	Asn483			sites Asn483 and Asn492				GnRH	2796	sites Asn483 and Asn492	Moreover, we showed that N-linked glycosylation sites Asn483 and Asn492 were vital for the transport function of Oatp1a5.
31526925	6	23	gly	sites	809:813	arg1	N-linked glycosylation sites			sites Asn483 and Asn492				GnRH	2796	sites Asn483 and Asn492	Moreover, we showed that N-linked glycosylation sites Asn483 and Asn492 were vital for the transport function of Oatp1a5.
31526925	6	23	gly	sites	809:813	arg1	N-linked glycosylation sites			sites Asn483 and Asn492				GnRH	2796	sites Asn483 and Asn492	Moreover, we showed that N-linked glycosylation sites Asn483 and Asn492 were vital for the transport function of Oatp1a5.
31526925	6	29	gly	glycosylation	795:807	arg2	Asn483			sites Asn483 and Asn492				GnRH	2796	sites Asn483 and Asn492	Moreover, we showed that N-linked glycosylation sites Asn483 and Asn492 were vital for the transport function of Oatp1a5.
31526925	6	29	gly	glycosylation	795:807	arg2	Asn492			sites Asn483 and Asn492				GnRH	2796	sites Asn483 and Asn492	Moreover, we showed that N-linked glycosylation sites Asn483 and Asn492 were vital for the transport function of Oatp1a5.
31526925	6	29	gly	glycosylation	795:807	arg2	Asn492			sites Asn483 and Asn492				GnRH	2796	sites Asn483 and Asn492	Moreover, we showed that N-linked glycosylation sites Asn483 and Asn492 were vital for the transport function of Oatp1a5.
30541852	7	66	gly	glycosylation	1027:1039	arg2	an N-linked glycosylation site			an N-linked glycosylation site						site	The ecotropic tropism exhibited by this envelope is due to the presence or absence of an N-linked glycosylation site in the first extracellular loop as well as the specific amino acid sequence of the extracellular domains of the receptor.
31831633	5	93	gly	glycoforms	818:827	arg1	aberrant MUC1 glycoforms	aberrant MUC1 glycoforms				OGER		MUC1	P15941		We analyzed the involvement of macrophage-associated cytokines in the induction of aberrant MUC1 glycoforms.
33947960	5	56	gly	N-glycosylation	1170:1184	arg2	ErbB2 N-glycosylation sites			ErbB2 N-glycosylation sites						sites	In-depth mass spectrometry-based glycomic and glycoproteomic analysis of ErbB2's ectodomain disclosed a site-specific glycosylation profile in GC cells, in which the ST6Gal1 sialyltransferase specifically targets ErbB2 N-glycosylation sites occurring within the receptor's trastuzumab-binding domain.
31860772	7	51	gly	detected	1338:1345	arg1	the cyst fluid regions AND specific nonfucosylated glycans			the cyst fluid regions	specific nonfucosylated glycans					regions	A polycystic kidney disease tissue was also characterized for N-glycan composition, with specific nonfucosylated glycans detected in the cyst fluid regions.
31860772	7	85	gly	nonfucosylated	1315:1328	arg1	specific nonfucosylated glycans				specific nonfucosylated glycans						A polycystic kidney disease tissue was also characterized for N-glycan composition, with specific nonfucosylated glycans detected in the cyst fluid regions.
29171040	6	5	gly	glycosylation	1292:1304	arg1	proteins	proteins				Fterm		proteins			Using mass spectrometry, we identified a group of proteins with O-linked glycosylation to be responsible for the necrotic debris-induced IL-1β secretion.
31336868	7	2	gly	β-glucosidase	2297:2309	arg1	L-ido-azepane	-glucosidase			L-ido-azepane	PUBTATOR		-glucosidase	6476		Furthermore, besides α-glucosidase inhibition, both miglitol (41a) and L-ido-azepane (41b) proved to be the strongest β-glucosidase inhibitors of the series with IC50 of 4 µM.
27690717	3	68	gly	N-glycosylated	677:690	arg1	3 sites			3 sites						sites	Using a combination of site-directed mutagenesis, western blot and patch-clamp recordings, we demonstrated that 3 sites in the extracellular loop of β2 subunit are N-glycosylated (N-X-T/S at N88, N96 and N119).
32366695	3	9	gly	SARS-CoV-2	438:447	arg1	22 N-linked glycan sequons	SARS			22 N-linked glycan sequons	OGER		SARS	P49591		The SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion.
28871230	1	96	gly	glycoprotein	411:422	arg1	glycoprotein glycopatterns	glycoprotein glycopatterns				Fterm		glycoprotein			Background: Chronic infection with HBV (CHB) or HCV (CHC) is the most common chronic viral hepatitis that can lead to cirrhosis and hepatocellular carcinoma in humans, their infections have distinct pathogenic processes, however, little is known about the difference of glycoprotein glycopatterns in serum between hepatitis B virus (HBV)- and hepatitis C virus (HCV)-infected patients.
32817340	2	20	gly	N-Glycosylation	235:249	arg1	the major structural proteins	the major structural proteins				Fterm		proteins			N-Glycosylation of the major structural proteins (archaellins) is important for their subsequent incorporation into the archaellum filament.
30620550	1	1	gly	proteins	185:192	arg1	O-linked N-acetylglucosamine	proteins			O-linked N-acetylglucosamine	Fterm		proteins			O-linked N-acetylglucosamine ( O-GlcNAc) is a ubiquitous post-translational modification of proteins and is essential for cell function.
34450331	9	3	gly	glycoproteins	1735:1747	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			SIGNIFICANCE: We describe a novel method termed limited deglycosylation assay (LDA), to probe conformational changes of glycoproteins on a systems-wide scale.
35140700	13	6	gly	mono-sialylated	2045:2059	arg1	mono-sialylated and disialylated N-glycans				mono-sialylated and disialylated N-glycans						Both frequencies of mono-sialylated and disialylated N-glycans were increased by IL-21 and IL-17A with decreased frequency of asialylation, and the expression of β-galactoside α-2,6-sialyltransferase 1 was upregulated by IL-21 and IL-17A.
35140700	13	105	gly	disialylated	2065:2076	arg1	mono-sialylated and disialylated N-glycans				mono-sialylated and disialylated N-glycans						Both frequencies of mono-sialylated and disialylated N-glycans were increased by IL-21 and IL-17A with decreased frequency of asialylation, and the expression of β-galactoside α-2,6-sialyltransferase 1 was upregulated by IL-21 and IL-17A.
29997173	1	15	gly	glycoprotein	171:182	arg1	a glycophosphatidylinositol (GPI)-anchored glycoprotein	a glycophosphatidylinositol (GPI)-anchored glycoprotein				Fterm		glycoprotein			CD52, a glycophosphatidylinositol (GPI)-anchored glycoprotein, is released in a soluble form following T cell activation and binds to the Siglec (sialic acid-binding Ig-like lectin)-10 receptor on T cells to suppress their function.
29997173	1	15	gly	glycoprotein	171:182	arg1	CD52	CD52				OGER		CD52	P31358		CD52, a glycophosphatidylinositol (GPI)-anchored glycoprotein, is released in a soluble form following T cell activation and binds to the Siglec (sialic acid-binding Ig-like lectin)-10 receptor on T cells to suppress their function.
29295913	3	96	gly	glycosylation	448:460	arg1	extracellular proteins	extracellular proteins				Fterm		proteins			Posttranslational glycosylation of extracellular proteins characterizes factors produced by the pathogenic yeast cells but not those of avirulent mycelia, correlating glycosylation with infection.
34450331	7	77	gly	glycoproteins	1384:1396	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			These results show that LDA accurately reports on systems-wide conformational changes of glycoproteins induced under controlled treatment regimes.
31600726	7	86	gly	glycoforms	1095:1104	arg1	purified recombinant FSH glycoforms	purified recombinant FSH glycoforms				PUBTATOR		FSH	14308		In this study, we isolated secondary follicles from pre-pubertal mice and treated them with 20- or 100 ng/mL purified recombinant FSH glycoforms for 1 h or 18-20 h. Analysis of phosphorylated PKA substrates showed that glycoforms were bioactive in follicles following 1-h treatment, although differential bioactivity was only observed with the 100 ng/mL dose.
31067000	0	90	gly	Glycans	9:15	arg1	the Variable Domain	Domain			Glycans	Fterm		Domain			N-Linked Glycans in the Variable Domain of IgG Anti-Citrullinated Protein Antibodies Predict the Development of Rheumatoid Arthritis.
30566828	9	63	gly	nonglycosylated	1118:1132	arg1	the nonglycosylated Asn-297			the nonglycosylated Asn-297				CTRP12	388581	Asn-297	Replacing the nonglycosylated Asn-297 with glutamine or alanine also increased CTRP12 cleavage.
32441515	2	21	gly	attached	296:303	arg1	proteins AND The glycans	proteins			The glycans	Fterm		proteins			The glycans attached to proteins represent an important determinant of the protein interaction-specificity and maintain the 3D structure of proteins.
30186544	2	59	gly	glycosylation	354:366	arg1	intracellular proteins	intracellular proteins				Fterm		proteins			O-linked β-N-acetylglucosamine (O-GlcNAc) glycosylation of intracellular proteins denotes a type of posttranslational modification that influences multiple cytoplasmic and nuclear protein functions.
32334683	7	37	gly	glycoproteins	1124:1136	arg1	several other common glycoproteins	several other common glycoproteins				Fterm		glycoproteins			Fourteen therapeutic monoclonal antibodies (mAbs), one Fc-fusion protein and several other common glycoproteins have been used to demonstrate that FTIR spectra of glycoproteins display spectral variations according to their glycan and monosaccharide compositions.
32334683	7	57	gly	glycoproteins	1189:1201	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Fourteen therapeutic monoclonal antibodies (mAbs), one Fc-fusion protein and several other common glycoproteins have been used to demonstrate that FTIR spectra of glycoproteins display spectral variations according to their glycan and monosaccharide compositions.
33177111	9	34	gly	CD55	1369:1372	arg1	O-linked desialylation	CD55			O-linked desialylation	OGER		CD55	P08174		We also demonstrated that O-linked desialylation of CD55 by ST3GAL1 silencing resulted in increased C3 deposition and complement-mediated lysis of breast cancer cells and enhanced sensitivity to antibody-dependent cell-mediated cytotoxicity.
33177111	9	72	gly	desialylation	1352:1364	arg1	CD55	CD55				OGER		CD55	P08174		We also demonstrated that O-linked desialylation of CD55 by ST3GAL1 silencing resulted in increased C3 deposition and complement-mediated lysis of breast cancer cells and enhanced sensitivity to antibody-dependent cell-mediated cytotoxicity.
29745666	7	68	gly	proteins/glycoproteins	1039:1060	arg1	proteins/glycoproteins	proteins/glycoproteins				Fterm		proteins/glycoproteins			Efficient extraction of proteins/glycoproteins from biological samples was achieved by combining SDC lysis buffer and beads beating cell disruption.
29755357	8	17	gly	N-glycosylation	1473:1487	arg1	LCN2	LCN2				PUBTATOR		LCN2	3934		In sum, our data indicate that the N-glycosylation of LCN2 is not required for proper secretion and exosome cargo recruitment in different cell types, but might be relevant to increase overall solubility.
34962767	3	7	gly	glycoproteins	711:723	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Despite significant progress in understanding the biochemistry of these enzymes, known O-glycoproteases have specificity constraints, such as inefficient cleavage of glycoproteins bearing sialylated O-glycans, high selectivity for certain types of glycoproteins, or protein sequence bias.
34962767	3	61	gly	sialylated	651:660	arg1	sialylated O-glycans				sialylated O-glycans						Despite significant progress in understanding the biochemistry of these enzymes, known O-glycoproteases have specificity constraints, such as inefficient cleavage of glycoproteins bearing sialylated O-glycans, high selectivity for certain types of glycoproteins, or protein sequence bias.
34962767	3	65	gly	glycoproteins	629:641	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Despite significant progress in understanding the biochemistry of these enzymes, known O-glycoproteases have specificity constraints, such as inefficient cleavage of glycoproteins bearing sialylated O-glycans, high selectivity for certain types of glycoproteins, or protein sequence bias.
31837192	6	12	gly	proteins	1126:1133	arg1	glycans	proteins			glycans	Fterm		proteins			To elucidate the so far uncharacterized mechanisms leading to the trimming of terminal galactose residues from glycans of secreted proteins, we studied a N. benthamiana BGAL known to be active in the apoplast (NbBGAL1).
33139572	1	54	gly	heterogeneity	260:272	arg1	glycan structure				glycan structure						Mammalian brain glycome remains a relatively poorly understood area compared to other large-scale "omics" studies, such as genomics and transcriptomics due to the inherent complexity and heterogeneity of glycan structure and properties.
33993882	9	50	gly	fucosylated	1683:1693	arg1	core fucosylated structures				core fucosylated structures						RESULTS In the brain region showing LPS-induced neuroinflammation, a significant decrease in the abundance of sialylated and core fucosylated structures was seen (approximately 7.5% and 8.5%, respectively), whereas oligomannose N-glycans were significantly increased (13.5%).
31726007	7	8	gly	glycosylation	1103:1115	arg2	the glycosylation sites			the glycosylation sites						sites	At all of the glycosylation sites, the relative levels of G- and GG-Hyl were markedly diminished, i.e., by ∼50-75%, in Sh collagen, and at five of these sites, the level of Lys hydroxylation was significantly increased.
30814666	9	39	gly	glycosylated	1317:1328	arg1	101 sites			101 sites						sites	Quantitative glycoproteomic analysis revealed that 101 sites on 67 proteins involved in Mtb fitness and survival were differentially glycosylated between the four lineages, among which 64% were cell envelope and membrane proteins.
30563903	5	87	gly	glycosylated	940:951	arg1	the highly glycosylated proline-rich region			the highly glycosylated proline-rich region						region	In addition, we map the cleavage by CpaA to two positions, 279-280 and 308-309, within the highly glycosylated proline-rich region of human fXII, and show that cleavage at the 308-309 site is responsible for inactivation of fXII.
29093093	7	118	gly	glycoprotein	1672:1683	arg1	transmembrane S glycoprotein	transmembrane S glycoprotein				Fterm		glycoprotein			This property distinguishes PDCoV S from recently characterized betacoronavirus S proteins and suggests that the S protein of enterotropic PDCoV has evolved to tolerate the protease-rich environment of the small intestine and to fine-tune its fusion activation to avoid premature triggering and reduction of infectivity.IMPORTANCE Coronaviruses use transmembrane S glycoprotein trimers to promote host attachment and fusion of the viral and cellular membranes.
34864058	7	39	gly	α1-3-fucosylated	1227:1242	arg1	the α1-3-fucosylated Lewis X (Lex) blood group antigen				the α1-3-fucosylated Lewis X (Lex) blood group antigen						Additionally, we find that IL-22 signaling regulates levels of the α1-3-fucosylated Lewis X (Lex) blood group antigen, and that this glycan epitope is primarily displayed on O-glycosylated intestinal epithelial glycoproteins.
34864058	7	45	gly	O-glycosylated	1334:1347	arg1	O-glycosylated intestinal epithelial glycoproteins	O-glycosylated intestinal epithelial glycoproteins				Fterm		glycoproteins			Additionally, we find that IL-22 signaling regulates levels of the α1-3-fucosylated Lewis X (Lex) blood group antigen, and that this glycan epitope is primarily displayed on O-glycosylated intestinal epithelial glycoproteins.
34864058	7	82	gly	glycoproteins	1371:1383	arg1	O-glycosylated intestinal epithelial glycoproteins	O-glycosylated intestinal epithelial glycoproteins				Fterm		glycoproteins			Additionally, we find that IL-22 signaling regulates levels of the α1-3-fucosylated Lewis X (Lex) blood group antigen, and that this glycan epitope is primarily displayed on O-glycosylated intestinal epithelial glycoproteins.
29859938	3	55	gly	serine	586:591	arg1	O-linked N-acetylglucosamine			serine and threonine residues	O-linked N-acetylglucosamine					serine and threonine residues	O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulator of intracellular signaling, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA).
29859938	3	61	gly	threonine	597:605	arg1	O-linked N-acetylglucosamine			serine and threonine residues	O-linked N-acetylglucosamine					serine and threonine residues	O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulator of intracellular signaling, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA).
32817316	2	93	gly	glycoprotein	330:341	arg1	the S-layer glycoprotein	the S-layer glycoprotein				Fterm		glycoprotein			Previously, we have demonstrated that the S-layer glycoprotein from probiotic Lactobacillus kefiri CIDCA 8348 (SLP-8348) is recognized by Mincle (macrophage inducible C-type lectin receptor), and its adjuvanticity depends on the integrity of its glycans.
33676668	7	54	gly	glycopeptide	1401:1412	arg2	glycopeptide enrichment			glycopeptide enrichment						glycopeptide	Moreover, the results of glycopeptide enrichment and glycosylation analysis from human serum also show egood enrichment selectivity from real biological samples.
28679762	4	117	gly	glycoprotein	785:796	arg1	a glycoprotein	a glycoprotein				Fterm		glycoprotein			By using a virus overlay assay, it was previously shown that the major AAV2 binding protein in membrane preparations of human cells corresponds to a glycoprotein with a molecular mass of 150 kDa.
28303575	10	7	gly	n-glycosylation	1220:1234	arg1	Dectin-1	Dectin-1				PUBTATOR		Dectin-1	Q9BXN2		We show here that n-glycosylation of Dectin-1 is crucial for its cell surface expression and consequently signal transduction.
32168410	13	11	gly	attached	1591:1598	arg2	FXIII-B AND the glycan moieties	FXIII-B			the glycan moieties	PUBTATOR		FXIII-B	2165		CONCLUSION Characterization of the glycan moieties attached to FXIII-B is reported for the first time.
34089345	2	55	gly	glycoprotein	506:517	arg1	the MR glycan and glycoprotein recognition				the MR glycan and glycoprotein recognition						Here we used the murine MR CTLD4-7 coupled to the Fc-portion of human IgG (MR-Fc) to investigate the MR glycan and glycoprotein recognition.
33197804	10	42	gly	fucosylated	1405:1415	arg1	fucosylated glycans				fucosylated glycans						Furthermore, the proportion of mucin glycan species expressed throughout life changed during the aging process, and aging tended to decrease the proportion of fucosylated glycans and increase the proportion of sialoglycans.
33516683	3	82	gly	glycoproteins	545:557	arg1	almost all glycoproteins	almost all glycoproteins				Fterm		glycoproteins			While almost all glycoproteins clinically linked to stenotic valvular modeling contain multiple sites for N-glycosylation, there are very few reports aimed at understanding how N-glycosylation contributes to the valve structure in disease.
33577335	5	8	gly	glycoforms	821:830	arg1	three IL-17A glycoforms	three IL-17A glycoforms				PUBTATOR		IL-17A	3605		A comparison of three IL-17A glycoforms in a normal human dermal fibroblast (NHDF) assay reveals dose-dependent interleukin-6-inducing activities in all cases, wherein the glycoform with sialyl undecasaccharides displays much weaker stimulatory effect than that of the GlcNAc- or GlcNAc(β1→4)GlcNAc-modified proteins.
33462887	4	21	gly	structures	651:660	arg1	extracellular domains			extracellular domains	extracellular domains		Site			domains	We first quantify the Core 1 and 2 O-glycan structures on the carbohydrate recognition and extracellular domains of the protein using sequential exoglycosidase sequencing.
33462887	4	21	gly	structures	651:660	arg1	the protein	protein			structures	Fterm		protein			We first quantify the Core 1 and 2 O-glycan structures on the carbohydrate recognition and extracellular domains of the protein using sequential exoglycosidase sequencing.
33462887	4	48	gly	protein	727:733	arg1	carbohydrate recognition	protein			carbohydrate recognition	Fterm		protein			We first quantify the Core 1 and 2 O-glycan structures on the carbohydrate recognition and extracellular domains of the protein using sequential exoglycosidase sequencing.
29302041	9	21	gly	glycosylation	1301:1313	arg2	the two N-linked glycosylation sites			the two N-linked glycosylation sites						sites	Secretion of Hgbβma or Hgbβmi chains was seen only after genetic mutation to introduce the two N-linked glycosylation sites present in Hgbε, but absent in the Hgbβ chains.
33677548	4	24	gly	glycoform	905:913	arg1	a specific site			a specific site						site	Depending on the experimental method, such a standard must be able to represent glycans as complete structures or as compositions, store not just single glycans but also represent glycoforms on a specific glycosylation side, deal with partially missing site information if no site mapping was performed, and store abundances or ratios of glycans within a glycoform of a specific site.
34662441	7	19	gly	glycosylation	1501:1513	arg1	the N51 site			the N51 site				prostate-specific membrane antigen	2346	N51 site position	In line with the limited accessibility of membrane-proximal sites, no glycosylation was observed at the N51 site positioned closest to the membrane.
31336133	3	4	gly	N-glycosylation	558:572	arg2	2 potential N-glycosylation sites			2 potential N-glycosylation sites						sites	The ANITVNITV peptide described by Perlmann and coworkers presents 9 amino acids and 2 potential N-glycosylation sites.
34358619	3	85	gly	glycoproteins	579:591	arg1	numerous cell surface glycoproteins	numerous cell surface glycoproteins				Fterm		glycoproteins			Although brain function and behavior are known to be regulated by the N-glycosylation state of numerous cell surface glycoproteins, our current understanding of brain glycosylation is limited, and glycan variations associated with functional brain regions remain largely unknown.
34358619	3	97	gly	N-glycosylation	532:546	arg1	numerous cell surface glycoproteins	numerous cell surface glycoproteins				Fterm		glycoproteins			Although brain function and behavior are known to be regulated by the N-glycosylation state of numerous cell surface glycoproteins, our current understanding of brain glycosylation is limited, and glycan variations associated with functional brain regions remain largely unknown.
34962767	10	1	gly	asialylated	1512:1522	arg1	sialylated and asialylated O-glycans				sialylated and asialylated O-glycans						Finally, IMPa efficiently cleaved peptides and proteins carrying sialylated and asialylated O-glycans of varying complexity.
34962767	10	6	gly	sialylated	1497:1506	arg1	sialylated and asialylated O-glycans				sialylated and asialylated O-glycans						Finally, IMPa efficiently cleaved peptides and proteins carrying sialylated and asialylated O-glycans of varying complexity.
34962767	10	39	gly	carrying	1488:1495	arg1	peptides AND sialylated and asialylated O-glycans			peptides	sialylated and asialylated O-glycans					peptides	Finally, IMPa efficiently cleaved peptides and proteins carrying sialylated and asialylated O-glycans of varying complexity.
31501520	5	1	gly	PKM2	739:742	arg1	O-GlcNAcylation	PKM2			O-GlcNAcylation	PUBTATOR		PKM2	5315		Under high glucose conditions, PKM2 is a target of OGA-associated acetyltransferase activity, which facilitates O-GlcNAcylation of PKM2 by O-GlcNAc transferase (OGT).
31875397	4	23	gly	glycosylated	616:627	arg1	glycosylated neuropeptides			glycosylated neuropeptides						neuropeptides	Here, we describe the discovery of glycosylated neuropeptides with an enrichment-free approach, taking advantage of signature oxonium ions produced in higher-energy collision dissociation (HCD) MS/MS spectra.
32858085	11	4	gly	glycoproteins	1707:1719	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			GENERAL SIGNIFICANCE Our study provides a possibility that HUGT1 play a role not only in sensing the misfolded glycoprotein but also in promoting folding of glycoproteins in the endoplasmic reticulum glycoprotein quality control.
32858085	11	25	gly	glycoprotein	1661:1672	arg1	the misfolded glycoprotein	the misfolded glycoprotein				Fterm		glycoprotein			GENERAL SIGNIFICANCE Our study provides a possibility that HUGT1 play a role not only in sensing the misfolded glycoprotein but also in promoting folding of glycoproteins in the endoplasmic reticulum glycoprotein quality control.
32858085	11	68	gly	glycoprotein	1750:1761	arg1	the endoplasmic reticulum glycoprotein quality control	the endoplasmic reticulum glycoprotein quality control				Fterm		glycoprotein			GENERAL SIGNIFICANCE Our study provides a possibility that HUGT1 play a role not only in sensing the misfolded glycoprotein but also in promoting folding of glycoproteins in the endoplasmic reticulum glycoprotein quality control.
32827291	5	12	gly	glycosylated	1077:1088	arg1	either partially or fully glycosylated B4GalT4				either partially or fully glycosylated B4GalT4						We show that KS decrease in A375 cells deficient in B4GalT4 activity can be rescued by overproduction of either partially or fully glycosylated B4GalT4 but not with N-glycan-depleted B4GalT4 version.
34631661	13	84	gly	glycosylation	2381:2393	arg1	recombinant human spike proteins	recombinant human spike proteins				PUBTATOR		spike proteins	43740568		Collectively, these data underscore the importance of characterizing the site-specific glycosylation of recombinant human spike proteins from HEK and CHO cells in order to better understand the impact of the production host on this complex and important protein used in research, diagnostics and vaccines.
28871230	2	126	gly	glycoproteins	611:623	arg1	letin-mediated affinity capture glycoproteins	letin-mediated affinity capture glycoproteins				Fterm		glycoproteins			Methods: A method combining the lectin microarrays, letin-mediated affinity capture glycoproteins, and MALDI-TOF/TOF-MS was employed to analyze serum protein glycopatterns and identify the glycan structures from patients with CHB (n = 54) or CHC(n = 47), and healthy volunteers (HV, n = 35).
32553552	7	4	gly	glycopeptides	1035:1047	arg2	160 glycopeptides			160 glycopeptides						glycopeptides	We report here a total of 160 glycopeptides, representing 25 N-glycan compositions associated with 70 sites on 35 glycoproteins.
32553552	7	55	gly	glycoproteins	1119:1131	arg1	35 glycoproteins	35 glycoproteins				Fterm		glycoproteins			We report here a total of 160 glycopeptides, representing 25 N-glycan compositions associated with 70 sites on 35 glycoproteins.
28687873	6	89	gly	sialylated	1078:1087	arg1	sialylated N-linked glycans				sialylated N-linked glycans						α2,6-linked sialic acid served as a high-affinity receptor for NDV and the ST6Gal I sialyltransferase that synthesizes α2-6 linkage of sialylated N-linked glycans in CHO-K1 cells promoted NDV binding and cytopathic effect.
28303575	7	27	gly	non-glycosylated	874:889	arg1	non-glycosylated isoform B	non-glycosylated isoform B				Fterm		isoform B			Glycosylated Dectin-1 isoform A was predominantly localized at the cell surface, non-glycosylated isoform B was retained intracellularly.
32946227	5	42	gly	glycopeptide	1055:1066	arg2	glycopeptide self-assembly			glycopeptide self-assembly						glycopeptide	A high-resolution X-ray crystallographic structure of the β-galactose-containing gelator, along with other results from spectroscopic, microscopic, and rheological experiments, indicate an unusual carbohydrate-aromatic CH-π bonding that promotes glycopeptide self-assembly.
31907993	3	28	gly	glycoprotein	420:431	arg1	The glycoprotein CD11b/CD18	The glycoprotein CD11b/CD18				Fterm		glycoprotein			The glycoprotein CD11b/CD18 plays a well-described role in regulating PMN transepithelial migration and PMN inflammatory functions.
32842538	0	44	gly	N-glycosylation	0:14	arg1	the Pre-Membrane Protein	the Pre-Membrane Protein				Fterm		Protein			N-glycosylation in the Pre-Membrane Protein Is Essential for the Zika Virus Life Cycle.
30250045	8	34	gly	glycosylated	1135:1146	arg1	the SKM9-2 epitope			the SKM9-2 epitope						epitope	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
30250045	8	52	gly	serine	1208:1213	arg1	two disialylated core 1 O-linked glycan-modified serine residues			serine residues, Ser893 and Ser900	two disialylated core 1 O-linked glycan-modified serine residues			HEG1	Q9ULI3	serine residues, Ser893 and Ser900	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
30250045	8	20	gly	contained	1149:1157	arg1	the SKM9-2 epitope AND two disialylated core 1 O-linked glycan-modified serine residues			the SKM9-2 epitope	two disialylated core 1 O-linked glycan-modified serine residues					epitope	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
30250045	8	0	gly	disialylated	1163:1174	arg1	Ser893			serine residues, Ser893 and Ser900				HEG1	Q9ULI3	serine residues, Ser893 and Ser900	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
30250045	8	0	gly	disialylated	1163:1174	arg1	two disialylated core 1 O-linked glycan-modified serine residues			serine residues, Ser893 and Ser900				HEG1	Q9ULI3	serine residues, Ser893 and Ser900	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
30250045	8	0	gly	disialylated	1163:1174	arg1	two disialylated core 1 O-linked glycan-modified serine residues			serine residues, Ser893 and Ser900				HEG1	Q9ULI3	serine residues, Ser893 and Ser900	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
30250045	8	34	gly	glycosylated	1135:1146	arg2	Ser897			Ser897				HEG1	Q9ULI3	Ser897	Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser897 was not glycosylated, contained two disialylated core 1 O-linked glycan-modified serine residues, Ser893 and Ser900.
32719555	4	22	gly	glycoforms	969:978	arg1	MUC1	MUC1				PUBTATOR		MUC1	4582		In addition, O-glycosylation-competent bacteria were able to generate an antigenically authentic Tn-MUC1 glycoform that exhibited reactivity with antibody 5E5, which specifically recognizes cancer-associated glycoforms of MUC1.
32719555	4	43	gly	glycoform	866:874	arg1	an antigenically authentic Tn-MUC1 glycoform	an antigenically authentic Tn-MUC1 glycoform				PUBTATOR		MUC1	4582		In addition, O-glycosylation-competent bacteria were able to generate an antigenically authentic Tn-MUC1 glycoform that exhibited reactivity with antibody 5E5, which specifically recognizes cancer-associated glycoforms of MUC1.
30127001	9	46	gly	site	1495:1498	arg1	serine 435			serine 435	serine 435		SpecificSite	POGLUT1	56983	serine 435	Mutation of the O-Glc modification site on EGF11 (serine 435) in combination with sensitizing O-fucose mutations in EGF8 or EGF12 affected cell-surface presentation of NOTCH1 or reduced activation of NOTCH1 by Delta-like1, respectively.
33657946	5	3	gly	fucosylated	804:814	arg1	fucosylated triantennary structures				fucosylated triantennary structures						Several parameters (low T stage, low Gleason score, low EAU risk groups for BCR, absence of positive surgical margins, high ratio of fucosylated triantennary structures on total of multiantennary structures [3AFc/MA], low ratio of fucosylated biantennary with core-branched N-acetylglucosamine on total of biantennary structures, and high ratio of triantennary structures on total of multiantennary structures) proved to have a univariate beneficial effect on BCR-free survival.
34110173	8	23	gly	glycosylation	1520:1532	arg1	a therapeutic glycoprotein	a therapeutic glycoprotein				Fterm		glycoprotein			Finally, this integrated strategy was proved to realize the in-depth glycosylation analysis of a therapeutic glycoprotein to understand its pharmacological properties and improving the manufacturing processes.
34110173	8	47	gly	glycoprotein	1560:1571	arg1	a therapeutic glycoprotein	a therapeutic glycoprotein				Fterm		glycoprotein			Finally, this integrated strategy was proved to realize the in-depth glycosylation analysis of a therapeutic glycoprotein to understand its pharmacological properties and improving the manufacturing processes.
31326749	1	32	gly	glycosylated	264:275	arg1	N-linked glycosylated proteins	N-linked glycosylated proteins				Fterm		proteins			NGLY1 deficiency is a rare inherited disorder caused by mutations in the NGLY1 gene encoding N-glycanase 1 that is a hydrolase for N-linked glycosylated proteins.
30657688	1	30	gly	proteins	306:313	arg1	O-GlcNAcylation	proteins			O-GlcNAcylation	Fterm		proteins			As a dynamic post-translational modification, O-linked β- N-acetylglucosamine ( O-GlcNAc) modification (i.e., O-GlcNAcylation) of proteins regulates many biological processes involving cellular metabolism and signaling.
31164895	4	74	gly	epitope	707:713	arg1	the α(1,3)-fucose epitope present				the α(1,3)-fucose epitope present						Meanwhile, the potential immunogenicity of the α(1,3)-fucose epitope present on plant-derived biopharmaceuticals is still a matter of debate.
28528272	5	52	gly	unglycosylated	632:645	arg1	unglycosylated proteins	unglycosylated proteins				Fterm		proteins			To obtain unglycosylated proteins, cells were treated with tunicamycin, an inhibitor of N-linked glycosylation, whereas PNGase F and Endo H were used to characterize the glycosylation types.
32719555	3	56	gly	O-glycoproteins	722:736	arg1	O-glycoproteins	O-glycoproteins				Fterm		O-glycoproteins			These same glycoengineered bacteria were used to supply crude cell extracts enriched with glycosylation machinery that permitted cell-free construction of O-glycoproteins in a one-pot reaction.
33610554	9	5	gly	structures	1332:1341	arg1	N-Cdh	Cdh			structures	OGER		Cdh	Q8NE62		In addition, site-specific changes in the N-glycan structures in the extracellular domain of N-Cdh were detected, which positively impact on homotypic interactions.
33610554	9	5	gly	structures	1332:1341	arg1	the extracellular domain			the extracellular domain	the extracellular domain		Site			domain	In addition, site-specific changes in the N-glycan structures in the extracellular domain of N-Cdh were detected, which positively impact on homotypic interactions.
32817316	4	59	gly	glycosylation	621:633	arg1	SLP-5818	SLP				PUBTATOR		SLP	100418288		Herein, we analyze the glycosylation pattern of three SLPs, SLP-8348, SLP-8321, and SLP-5818, and explore how these patterns impact their recognition by C-type lectin receptors and the immunomodulatory effect of the L. kefiri SLPs on antigen-presenting cells.
32817316	4	59	gly	glycosylation	621:633	arg1	three SLPs	SLPs, SLP				PUBTATOR		SLPs, SLP	100418288		Herein, we analyze the glycosylation pattern of three SLPs, SLP-8348, SLP-8321, and SLP-5818, and explore how these patterns impact their recognition by C-type lectin receptors and the immunomodulatory effect of the L. kefiri SLPs on antigen-presenting cells.
30566828	7	55	gly	N-glycans	838:846	arg1	CTRP12	CTRP12			N-glycans	PUBTATOR		CTRP12	388581		Complex-type N-glycans on CTRP12 blocked cleavage by the Golgi-localized furin.
34279906	6	66	gly	glycoprotein	1388:1399	arg1	glycoprotein function	glycoprotein function				Fterm		glycoprotein			Taken together, native exoglycosidase sequencing mass spectrometry (NES-MS) notably improves our ability to characterize protein glycosylation, addressing a significant need in structural biology that will enable new routes to understand glycoprotein function.
29842980	9	51	gly	glycoprotein	1448:1459	arg1	VP7 glycoprotein	VP7 glycoprotein				Fterm		glycoprotein			Presence of additional N-linked glycosylation site at amino acid 283 of VP7 glycoprotein suggests that the major neutralizing epitope on the VP7 (G3) of RotaTeq vaccine differs from the currently circulating G3 strains.
29842980	9	39	gly	glycosylation	1404:1416	arg1	VP7 glycoprotein	glycoprotein		site		Fterm		glycoprotein		site	Presence of additional N-linked glycosylation site at amino acid 283 of VP7 glycoprotein suggests that the major neutralizing epitope on the VP7 (G3) of RotaTeq vaccine differs from the currently circulating G3 strains.
31101650	1	58	gly	glycoproteins	253:265	arg1	specific glycoproteins	specific glycoproteins				Fterm		glycoproteins			Global inhibition of N-linked glycosylation broadly reduces glycan occupancy on glycoproteins, but identifying how this inhibition functionally impacts specific glycoproteins is challenging.
31101650	1	63	gly	glycoproteins	172:184	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Global inhibition of N-linked glycosylation broadly reduces glycan occupancy on glycoproteins, but identifying how this inhibition functionally impacts specific glycoproteins is challenging.
31101650	1	81	gly	occupancy	159:167	arg1	glycoproteins	glycoproteins			occupancy	Fterm		glycoproteins			Global inhibition of N-linked glycosylation broadly reduces glycan occupancy on glycoproteins, but identifying how this inhibition functionally impacts specific glycoproteins is challenging.
31266803	5	30	gly	defucosylate	1074:1085	arg1	LewisY and LewisB antigens				LewisY and LewisB antigens						Together, these enzymes could defucosylate LewisY and LewisB antigens in a complementary fashion.
31168022	3	32	gly	glycoprotein	369:380	arg1	GP	GP				PUBTATOR		GP	55819		MLS128 mAb inhibited cell growth and bound to a 110 kDa glycoprotein (GP) in LS180 and HT29 colon cancer cells.
31168022	3	32	gly	glycoprotein	369:380	arg1	a 110 kDa glycoprotein	a 110 kDa glycoprotein				Fterm		glycoprotein			MLS128 mAb inhibited cell growth and bound to a 110 kDa glycoprotein (GP) in LS180 and HT29 colon cancer cells.
30487280	15	85	gly	glycoproteins	2659:2671	arg1	envelope glycoproteins	envelope glycoproteins				Fterm		glycoproteins			Thus, in principle, the antibody response might be diverted from one site to a more cross-reactive one, which would help in the induction of broadly neutralizing antibodies by HIV-1 vaccines based on envelope glycoproteins.
31665542	3	15	gly	N-glycosylation	522:536	arg1	S-layer proteins	S-layer proteins				Fterm		proteins			Here, we investigated N-glycosylation of S-layer proteins, the major archaeal envelope component, with respect to mesophilic adaptation.
28410396	6	71	gly	glycosylation	1441:1453	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Phylogenetic analysis of the influenza virus strains from this study and elsewhere around the world, together with the estimations of nucleotide substitution rates and selection pressure, and the predictions of B-cell epitopes and N-linked glycosylation sites all provided evidence for the ongoing evolution of NA.
34450331	1	27	gly	glycoproteins	147:159	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			A new method to probe the conformational changes of glycoproteins on a systems-wide scale, termed limited deglycosylation assay (LDA), is described.
31079966	0	16	gly	O-mannosylated	85:98	arg1	O-mannosylated position			O-mannosylated position						position	Synthetic glycopeptides reveal specific binding pattern and conformational change at O-mannosylated position of α-dystroglycan by POMGnT1 catalyzed GlcNAc modification.
31079966	0	45	gly	glycopeptides	10:22	arg2	Synthetic glycopeptides			Synthetic glycopeptides						glycopeptides	Synthetic glycopeptides reveal specific binding pattern and conformational change at O-mannosylated position of α-dystroglycan by POMGnT1 catalyzed GlcNAc modification.
31892091	6	13	gly	N83-glycopeptides	1281:1297	arg2	N83-glycopeptides			N83-glycopeptides						N83	Although optimized for O-glycopeptides, the method proved also successful for preconcentration of N83-glycopeptides, without compromising the separation between glycopeptide glycoforms with different number of sialic acids.
31892091	6	29	gly	O-glycopeptides	1206:1220	arg2	O-glycopeptides			O-glycopeptides						O-glycopeptides	Although optimized for O-glycopeptides, the method proved also successful for preconcentration of N83-glycopeptides, without compromising the separation between glycopeptide glycoforms with different number of sialic acids.
31892091	6	55	gly	glycopeptide	1344:1355	arg2	glycopeptide			glycopeptide						glycopeptide	Although optimized for O-glycopeptides, the method proved also successful for preconcentration of N83-glycopeptides, without compromising the separation between glycopeptide glycoforms with different number of sialic acids.
32364434	3	66	gly	glycoproteins	624:636	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Thus, there is increasing demand for the qualitative and quantitative characterization and validation of glycosylation on glycoproteins.
32364434	3	71	gly	glycosylation	607:619	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Thus, there is increasing demand for the qualitative and quantitative characterization and validation of glycosylation on glycoproteins.
29782851	1	45	gly	glycoproteins	119:131	arg1	secreted glycoproteins	secreted glycoproteins				Fterm		glycoproteins			Cbln1 is the prototype of a family (Cbln1-Cbln4) of secreted glycoproteins and is essential for normal synapse structure and function in cerebellum by bridging presynaptic Nrxn to postsynaptic Grid2.
33462887	2	38	gly	glycosylation	354:366	arg1	DC-SIGN	DC-SIGN				OGER		DC-SIGN	Q9NNX6		How these recognition events take place with different viruses is not clear and the effects of glycosylation on the folding and stability of DC-SIGN have not been reported.
31511323	6	20	gly	glycosylation	1058:1070	arg1	efficient β2 trafficking	efficient β2 trafficking				PUBTATOR		2	170589		We found that fully nonglycosylated β2 was mostly retained in the endoplasmic reticulum, indicating that N-linked glycosylation is required for efficient β2 trafficking to the apical plasma membrane.
31511323	6	83	gly	nonglycosylated	964:978	arg1	fully nonglycosylated β2	fully nonglycosylated β2				PUBTATOR		2	170589		We found that fully nonglycosylated β2 was mostly retained in the endoplasmic reticulum, indicating that N-linked glycosylation is required for efficient β2 trafficking to the apical plasma membrane.
32825463	10	57	gly	fucosylated	1229:1239	arg1	fucosylated glycans				fucosylated glycans						These findings demonstrate that labeling and detection of fucosylated glycans with Fuc analogs depend on multiple cellular steps, including conversion to GDP form, transport into the ER or Golgi, and utilization by each fucosyltransferase, providing insights into design of novel sugar analogs for specific detection of target glycans or inhibition of their functions.
31600726	9	49	gly	glycoforms	1545:1554	arg1	FSH glycoforms	FSH glycoforms				PUBTATOR		FSH	14308		Our results, therefore, indicate that FSH glycoforms are bioactive in isolated murine follicles.
31450586	8	89	gly	glycopeptides	1051:1063	arg2	118 unique N-linked glycopeptides			118 unique N-linked glycopeptides						glycopeptides	Additionally, we identified 118 unique N-linked glycopeptides corresponding to 84 proteins to be differentially glycosylated only in non-type subtypes as compared to luminal/basal subtypes.
31931833	3	75	gly	N-glycoproteins	404:418	arg1	defined N-glycans	N-glycoproteins			defined N-glycans	Fterm		N-glycoproteins			Thus homogeneous N-glycoproteins with defined N-glycans are essential in their application in clinic therapeutics.
29385488	2	96	gly	used	527:530	arg2	composition			composition						position	Ten ingredients that vary in fiber concentration and composition were used: corn, wheat, soybean meal (SBM), canola meal, distillers dried grains with solubles (DDGS), corn germ meal, copra expellers, sugar beet pulp (SBP), synthetic cellulose (SF), and pectin.
30127001	3	42	gly	NOTCH1	497:502	arg1	Epidermal Growth Factor-like (EGF) repeat 11	NOTCH1			Epidermal Growth Factor-like (EGF) repeat 11	OGER		NOTCH1	P46531		This serine occurs between conserved cysteines 3 and 4 of Epidermal Growth Factor-like (EGF) repeat 11 of NOTCH1, a site distinct from those modified by protein O-glucosyltransferase 1 (POGLUT1), suggesting that a different enzyme is responsible.
30919021	0	61	gly	glycans	25:31	arg1	prolyl 4-hydroxylase subunit	prolyl 4-hydroxylase subunit α1			glycans	PUBTATOR		prolyl 4-hydroxylase subunit α1	18451		Ascorbate inducible N259 glycans on prolyl 4-hydroxylase subunit α1 promote hydroxylation and secretion of type I collagen.
34229070	5	104	gly	O-glycosites	775:786	arg2	various O-glycosites			various O-glycosites						O-glycosites	We calculated relative abundance of glycopeptides with various O-glycosites and O-glycans, and further investigated the alteration of APP O-glycosylation upon TNF-α treatment.
34229070	5	73	gly	glycopeptides	748:760	arg1	O-glycans			glycopeptides	O-glycans					glycopeptides	We calculated relative abundance of glycopeptides with various O-glycosites and O-glycans, and further investigated the alteration of APP O-glycosylation upon TNF-α treatment.
29717387	5	48	gly	N-glycosylation	614:628	arg1	PPARγ	PPARγ				OGER		PPAR	Q07869		Disruption of both sites by site-directed mutagenesis completely abrogated the N-glycosylation of PPARγ.
32699088	13	55	gly	glycosylation	2437:2449	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	These results demonstrate that in addition to antigenicity, changes in N-linked glycosylation sites can alter other properties of viral surface antigens and virions.
30713024	6	23	gly	OGT-dependent	937:949	arg1	O-GlcNAcF3	OGT			O-GlcNAcF3	PUBTATOR		OGT	108155		Further, NIH3T3 cells interfered with OGT (siOGT) showed significant decreasing of O-GlcNAc levels with Ac4GlcNAcF3 treatment, indicating O-GlcNAcF3 was an OGT-dependent modification.
30956133	4	6	gly	α2,6-sialyltransferase	414:435	arg1	ST6Gal-I	-galactoside α2,6-sialyltransferase 1			ST6Gal-I	PUBTATOR		-galactoside α2,6-sialyltransferase 1	6480		We identify distinct functions of exomeres mediated by two of their cargo, the β-galactoside α2,6-sialyltransferase 1 (ST6Gal-I) that α2,6- sialylates N-glycans, and the EGFR ligand, amphiregulin (AREG).
30956133	4	8	gly	β-galactoside	400:412	arg1	ST6Gal-I	-galactoside α2,6-sialyltransferase 1			ST6Gal-I	PUBTATOR		-galactoside α2,6-sialyltransferase 1	6480		We identify distinct functions of exomeres mediated by two of their cargo, the β-galactoside α2,6-sialyltransferase 1 (ST6Gal-I) that α2,6- sialylates N-glycans, and the EGFR ligand, amphiregulin (AREG).
30956133	4	45	gly	sialylates	461:470	arg1	the β-galactoside α2,6-sialyltransferase 1	-galactoside α2,6-sialyltransferase 1			ST6Gal-I	PUBTATOR		-galactoside α2,6-sialyltransferase 1	6480		We identify distinct functions of exomeres mediated by two of their cargo, the β-galactoside α2,6-sialyltransferase 1 (ST6Gal-I) that α2,6- sialylates N-glycans, and the EGFR ligand, amphiregulin (AREG).
30956133	4	45	gly	sialylates	461:470	arg1	the β-galactoside α2,6-sialyltransferase 1	-galactoside α2,6-sialyltransferase 1			N-glycans	PUBTATOR		-galactoside α2,6-sialyltransferase 1	6480		We identify distinct functions of exomeres mediated by two of their cargo, the β-galactoside α2,6-sialyltransferase 1 (ST6Gal-I) that α2,6- sialylates N-glycans, and the EGFR ligand, amphiregulin (AREG).
31722217	3	38	gly	sites	626:630	arg1	three sites			three sites						sites	Using a variety of assays, we find important roles in Drosophila Notch signaling for GlcNAc-fucose-O glycans on three sites: EGF8, EGF9, and EGF12.
31029427	7	35	gly	used	1150:1153	arg2	synthesized peptides			synthesized peptides						peptides	Then, synthesized peptides, including the predicted O-GalNAc sites in the C-terminus of FOXA1, were used in a vitro reaction, and peptides mutated at the predicted O-GalNAc sites were employed as controls.
29609090	3	27	gly	glycopeptide	535:546	arg2	glycopeptide formation			glycopeptide formation						glycopeptide	RP-HPLC and Mass Spectrometry were used to assay and quantify glycopeptide formation by AaNGT and determine its substrate specificities.
33177111	7	25	gly	CD55	952:955	arg1	N-	CD55			N-	OGER		CD55	P08174		Detailed analyses of N- and O-linked oligosaccharides of CD55 released from scramble or ST3GAL1 siRNA-treated breast cancer cells by tandem mass spectrometry revealed that the N-glycan profile was not affected by ST3GAL1 silencing.
33177111	7	25	gly	CD55	952:955	arg1	O-linked oligosaccharides	CD55			O-linked oligosaccharides	OGER		CD55	P08174		Detailed analyses of N- and O-linked oligosaccharides of CD55 released from scramble or ST3GAL1 siRNA-treated breast cancer cells by tandem mass spectrometry revealed that the N-glycan profile was not affected by ST3GAL1 silencing.
33340519	7	57	gly	deglycosylation	1025:1039	arg1	ACE2	ACE2				OGER		ACE2	Q9BYF1		In contrast, deglycosylation of ACE2 did not influence SARS-CoV-2 binding.
32986846	4	14	gly	asparagine-linked	694:710	arg1	the asparagine-linked (N-linked) glycan profile			asparagine	the asparagine-linked (N-linked) glycan profile					asparagine	As a consequence, we hypothesized that the glycan profile is altered in the brain of patients with AD and analyzed the asparagine-linked (N-linked) glycan profile in hippocampus and cortex in AD and control brain.
29988048	8	43	gly	O-glycosylation	1017:1031	arg2	this O-glycosylation site			this O-glycosylation site						site	Mass spectrometric analysis indicates that this O-glycosylation site is heterogeneously occupied in VSG3 by zero to three hexose residues and is also present in other VSGs.
29988048	8	45	gly	occupied	1057:1064	arg2	this O-glycosylation site			this O-glycosylation site						site	Mass spectrometric analysis indicates that this O-glycosylation site is heterogeneously occupied in VSG3 by zero to three hexose residues and is also present in other VSGs.
31498587	3	36	gly	glycopeptides	439:451	arg2	MUC1 glycopeptides SAPDT*RPAP (T* is the glycosylation site)			MUC1 glycopeptides SAPDT*RPAP (T* is the glycosylation site)						glycopeptides	In order to target these antigens as potential anticancer vaccines, MUC1 glycopeptides SAPDT*RPAP (T* is the glycosylation site) bearing the Tf and the STn antigen, respectively, have been synthesized.
31498587	3	78	gly	glycosylation	475:487	arg2	the glycosylation site			the glycosylation site						site	In order to target these antigens as potential anticancer vaccines, MUC1 glycopeptides SAPDT*RPAP (T* is the glycosylation site) bearing the Tf and the STn antigen, respectively, have been synthesized.
31498587	3	90	gly	RPAP	459:462	arg1	the glycosylation site			the glycosylation site						site	In order to target these antigens as potential anticancer vaccines, MUC1 glycopeptides SAPDT*RPAP (T* is the glycosylation site) bearing the Tf and the STn antigen, respectively, have been synthesized.
31931833	9	64	gly	glycoprotein	1765:1776	arg1	increasing novel glycoprotein drugs	increasing novel glycoprotein drugs				Fterm		glycoprotein			This system would provide a prospective platform for mass production of increasing novel glycoprotein drugs.
28348171	9	62	gly	glycoproteins	1536:1548	arg1	these surface glycoproteins	these surface glycoproteins				Fterm		glycoproteins			Finally, we determined whether these surface glycoproteins, found enriched on M2 macrophages, were also expressed in human metastatic castrate-resistant prostate cancer (mCRPC) tissues.
30919021	5	2	gly	glycosylation	577:589	arg1	P4HA1	P4HA1				PUBTATOR		P4HA1	18451		N259 glycosylation on P4HA1 correlates with enhanced pepsin-resistant collagen 1α2 secretion.
29609090	4	31	gly	glycosylate	750:760	arg1	the tested peptides			the tested peptides						peptides	AaNGT could utilize a variety of nucleotide-activated sugar donors, including UDP-Glc, UDP-Gal, UDP-Xyl, GDP-Glc, dGDP-Glc and UDP-GlcN, to glycosylate the tested peptides.
29708975	8	11	gly	glycosylation	1118:1130	arg2	an extra N-linked glycosylation site			an extra N-linked glycosylation site						site	Pakistani G3 strains belonged to lineage 3 within sub-lineage 3d, containing an extra N-linked glycosylation site compared to the G3 strain of RotaTeqTM.
29672582	2	44	gly	IgG	491:493	arg1	G0 N-glycan	IgG			G0 N-glycan	PUBTATOR		IgG	668542		Many reports show that altered IgG glycosylation, especially IgG with agalactosylated N-linked glycan (G0 N-glycan), have proinflammatory roles including complement activation, implicated in the pathogenesis of various inflammatory diseases.
29672582	2	44	gly	IgG	491:493	arg1	agalactosylated N-linked glycan	IgG			agalactosylated N-linked glycan	PUBTATOR		IgG	668542		Many reports show that altered IgG glycosylation, especially IgG with agalactosylated N-linked glycan (G0 N-glycan), have proinflammatory roles including complement activation, implicated in the pathogenesis of various inflammatory diseases.
32452486	4	29	gly	glycans	634:640	arg1	a protein	protein			glycans	Fterm		protein			Herein we present a simple and new strategy for targeting the glycans on a protein (with IgG as a model glycoprotein) using surface-enhanced Raman scattering (SERS) coupled to glycan-binding WGA (wheat germ agglutinin) lectin, in a lectin-SERS assay.
32452486	4	42	gly	glycoprotein	676:687	arg1	a model glycoprotein	a model glycoprotein				Fterm		glycoprotein			Herein we present a simple and new strategy for targeting the glycans on a protein (with IgG as a model glycoprotein) using surface-enhanced Raman scattering (SERS) coupled to glycan-binding WGA (wheat germ agglutinin) lectin, in a lectin-SERS assay.
31702909	8	71	gly	glycosylation	1300:1312	arg2	all N-linked glycosylation sites			all N-linked glycosylation sites						sites	A designed CD19 variant with all N-linked glycosylation sites removed successfully bound antibody in the yeast display context, which provides a lead for aglycosylated applications.
29339807	7	30	gly	fucosylated	1399:1409	arg1	fucosylated enzyme	fucosylated enzyme				Fterm		enzyme			To our knowledge, this is the first study reporting the functional role of fucosylated enzyme in the development of castration-resistant prostate cancer.
31498587	10	48	gly	glycopeptide	1451:1462	arg2	Qβ-MUC1 glycopeptide conjugates			Qβ-MUC1 glycopeptide conjugates						glycopeptide	As such, Qβ-MUC1 glycopeptide conjugates have great potential as anticancer vaccines.
30395637	2	42	gly	glycoprotein	320:331	arg1	trimeric HIV-1 envelope (Env) glycoprotein	trimeric HIV-1 envelope (Env) glycoprotein				Fterm		glycoprotein			VRC01-class bnAbs that target the CD4 binding site (CD4bs) of trimeric HIV-1 envelope (Env) glycoprotein spikes are particularly attractive to elicit because of their extraordinary breadth and potency of neutralization in vitro and their ability to protect against infection in animal models.
33556394	6	42	gly	Ser41	1099:1103	arg1	an O-glycan modification			Ser41	an O-glycan modification			β1-adrenergic		Ser41	We identify Ser41 as the major O-glycosylation site on the β1AR N-terminus and show that an O-glycan modification at Ser41 prevents ADAM17-dependent cleavage of the β1-AR N-terminus at S41↓L42, a second N-terminal cleavage site adjacent to this O-glycan modification (and it attenuates β1-AR N-terminal cleavage at R31↓L32).
33556394	6	47	gly	O-glycosylation	1013:1027	arg2	the major O-glycosylation site			the major O-glycosylation site						site	We identify Ser41 as the major O-glycosylation site on the β1AR N-terminus and show that an O-glycan modification at Ser41 prevents ADAM17-dependent cleavage of the β1-AR N-terminus at S41↓L42, a second N-terminal cleavage site adjacent to this O-glycan modification (and it attenuates β1-AR N-terminal cleavage at R31↓L32).
33556394	6	47	gly	O-glycosylation	1013:1027	arg2	Ser41			Ser41				β1-adrenergic		Ser41	We identify Ser41 as the major O-glycosylation site on the β1AR N-terminus and show that an O-glycan modification at Ser41 prevents ADAM17-dependent cleavage of the β1-AR N-terminus at S41↓L42, a second N-terminal cleavage site adjacent to this O-glycan modification (and it attenuates β1-AR N-terminal cleavage at R31↓L32).
31710461	5	9	gly	glycosylated	853:864	arg1	partially glycosylated Asn sites			partially glycosylated Asn sites						Asn sites	ChyB-Asn enabled the identification of partially glycosylated Asn sites within a model glycoprotein and in the extracellular proteome of Jurkat T cells.
31710461	5	51	gly	glycoprotein	891:902	arg1	a model glycoprotein	a model glycoprotein				Fterm		glycoprotein			ChyB-Asn enabled the identification of partially glycosylated Asn sites within a model glycoprotein and in the extracellular proteome of Jurkat T cells.
34864058	9	34	gly	fucosylation	1690:1701	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Together, these data identify B3GNT7 as an intermediary in IL-22-dependent induction of fucosylation of glycoproteins and uncover a novel role for B3GNT7 in intestinal glycosylation.
34864058	9	73	gly	glycoproteins	1706:1718	arg1	fucosylation	glycoproteins			fucosylation	Fterm		glycoproteins			Together, these data identify B3GNT7 as an intermediary in IL-22-dependent induction of fucosylation of glycoproteins and uncover a novel role for B3GNT7 in intestinal glycosylation.
31527085	7	5	gly	sites	816:820	arg1	the tetratricopeptide repeat domain			the tetratricopeptide repeat domain	the tetratricopeptide repeat domain						We identified six O-GlcNAc sites in the tetratricopeptide repeat domain in sOGT, with Thr12 and Ser56 being two "key" sites.
31527085	7	30	gly	domain	854:859	arg1	sOGT	sOGT			domain	Cterm		sOGT	8473		We identified six O-GlcNAc sites in the tetratricopeptide repeat domain in sOGT, with Thr12 and Ser56 being two "key" sites.
33116178	5	9	gly	fucosylated	714:724	arg1	highly fucosylated N-glycans				highly fucosylated N-glycans						To unravel the synthesis of highly fucosylated N-glycans by S. mansoni, we examined the ability of ten selected SmFucTs to modify N-glycans upon transient expression in Nicotiana benthamiana plants.
32887075	8	58	gly	N-glycans	1390:1398	arg1	other sites			other sites	other sites		Site			sites	N-glycans in other sites were mainly complex-type with a low content of fucosylation and sialylated.
32887075	8	72	gly	sialylated	1479:1488	arg1	N-glycans				N-glycans						N-glycans in other sites were mainly complex-type with a low content of fucosylation and sialylated.
30755292	7	77	gly	glycosylation	1354:1366	arg2	one N-linked glycosylation site			one N-linked glycosylation site						site	The isolates in clades 3C.2A1a and 3C.2A3 lost one N-linked glycosylation site in HA relative to other clades.
29886537	2	40	gly	glycosylation	278:290	arg1	amyloid precursor protein	amyloid precursor protein				OGER		amyloid precursor protein	P05067		They include N-linked glycosylation of the prion protein and amyloid precursor protein, phosphorylation of tau and α-synuclein.
29886537	2	40	gly	glycosylation	278:290	arg1	the prion protein	the prion protein				Fterm		protein			They include N-linked glycosylation of the prion protein and amyloid precursor protein, phosphorylation of tau and α-synuclein.
34401920	10	44	gly	glycoprotein	1403:1414	arg1	three interacting glycoprotein networks	three interacting glycoprotein networks				Fterm		glycoprotein			Glycoside hydrases, serine proteases and serpins were found to form three interacting glycoprotein networks using the website STRING.
34110173	6	13	gly	glycopeptide	1242:1253	arg2	glycopeptide analysis			glycopeptide analysis						glycopeptide	In total, 114 N-glycan compositions were identified from glycopeptide analysis.
32764711	2	43	gly	glycoprotein	306:317	arg1	the TBEV envelope (E) glycoprotein	the TBEV envelope (E) glycoprotein				Fterm		glycoprotein			N-Glycosylation of the TBEV envelope (E) glycoprotein is critical for virus egress in mammalian cells, but not in tick cells.
32764711	2	53	gly	N-Glycosylation	265:279	arg1	the TBEV envelope (E) glycoprotein	the TBEV envelope (E) glycoprotein				Fterm		glycoprotein			N-Glycosylation of the TBEV envelope (E) glycoprotein is critical for virus egress in mammalian cells, but not in tick cells.
34450331	3	23	gly	N-deglycosylation	576:592	arg1	formerly N-glycosylated peptides			formerly N-glycosylated peptides						peptides	LDA involves 1) protein-level N-deglycosylation under native conditions, 2) trypsin digestion, 3) glycopeptide enrichment, 4) peptide-level N-deglycosylation and 5) quantitative MS-based analysis of formerly N-glycosylated peptides (FNGPs).
34450331	3	37	gly	N-glycosylated	754:767	arg1	formerly N-glycosylated peptides			formerly N-glycosylated peptides						peptides	LDA involves 1) protein-level N-deglycosylation under native conditions, 2) trypsin digestion, 3) glycopeptide enrichment, 4) peptide-level N-deglycosylation and 5) quantitative MS-based analysis of formerly N-glycosylated peptides (FNGPs).
34450331	3	122	gly	N-deglycosylation	686:702	arg1	formerly N-glycosylated peptides			formerly N-glycosylated peptides						peptides	LDA involves 1) protein-level N-deglycosylation under native conditions, 2) trypsin digestion, 3) glycopeptide enrichment, 4) peptide-level N-deglycosylation and 5) quantitative MS-based analysis of formerly N-glycosylated peptides (FNGPs).
34450331	3	131	gly	glycopeptide	644:655	arg2	3) glycopeptide enrichment			3) glycopeptide enrichment						glycopeptide	LDA involves 1) protein-level N-deglycosylation under native conditions, 2) trypsin digestion, 3) glycopeptide enrichment, 4) peptide-level N-deglycosylation and 5) quantitative MS-based analysis of formerly N-glycosylated peptides (FNGPs).
30659065	2	7	gly	N-glycosylation	331:345	arg1	the N-linked glycans			domain	the N-linked glycans					domain	The isotype/subclass of the antibody, the co-translational N-glycosylation on the CH2 domain, and the remodeling of the N-linked glycans during passage through the ER and Golgi are the known variables within the Fc domain that program antibody effector function.
30659065	2	25	gly	N-glycosylation	331:345	arg1	the CH2 domain			domain						domain	The isotype/subclass of the antibody, the co-translational N-glycosylation on the CH2 domain, and the remodeling of the N-linked glycans during passage through the ER and Golgi are the known variables within the Fc domain that program antibody effector function.
30659065	2	25	gly	N-glycosylation	331:345	arg1	the CH2 domain			domain	the N-linked glycans					domain	The isotype/subclass of the antibody, the co-translational N-glycosylation on the CH2 domain, and the remodeling of the N-linked glycans during passage through the ER and Golgi are the known variables within the Fc domain that program antibody effector function.
33806155	6	10	gly	O-glycosites	1078:1089	arg2	16 out of 25 O-glycosites			16 out of 25 O-glycosites						O-glycosites	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.
33806155	6	49	gly	N-glycosites	1140:1151	arg2	known N-glycosites			known N-glycosites						N-glycosites	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.
32461612	4	25	gly	density	553:559	arg1	MERS S	S			density	Cterm		S	43740568		Here, we reveal a specific area of high glycan density on MERS S that results in the formation of oligomannose-type glycan clusters, which were absent on SARS and HKU1 CoVs.
31110306	3	47	gly	N-glycosylation	496:510	arg1	a stably expressed IgG	a stably expressed IgG				Cterm		IgG			In this study, we engineered Chinese hamster ovary cells with synthetic genetic circuits to tune N-glycosylation of a stably expressed IgG.
30002126	10	37	gly	glycosylation	1732:1744	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Collectively, our data suggest that factor VIII produced in baby hamster kidney cells is more immunogenic than that produced in Chinese hamster ovary cells, and that incomplete occupancy of N-linked glycosylation sites leads to the formation of IgM- and IgG-factor VIII immune complexes that contribute to the enhanced clearance and immunogenicity in these mouse models of hemophilia A.
30002126	10	103	gly	occupancy	1710:1718	arg2	N-linked glycosylation sites			N-linked glycosylation sites						sites	Collectively, our data suggest that factor VIII produced in baby hamster kidney cells is more immunogenic than that produced in Chinese hamster ovary cells, and that incomplete occupancy of N-linked glycosylation sites leads to the formation of IgM- and IgG-factor VIII immune complexes that contribute to the enhanced clearance and immunogenicity in these mouse models of hemophilia A.
33030205	7	58	gly	glycosylated	1578:1589	arg1	specific glycosylated motifs			specific glycosylated motifs						motifs	This structural evidence provides unprecedented insight into how the ancillary domains in glycoprotein-specific peptidases can noncatalytically recognize specific glycosylated motifs that are common in mucin and mucin-like molecules.
28528272	6	43	gly	glycosylated	839:850	arg1	glycosylated, unglycosylated proteins	glycosylated, unglycosylated proteins				Fterm		proteins			Cells were incubated with glycosylated, unglycosylated proteins and/or legumain selective activity-based probe, and legumain and/or cystatin E/M was studied by activity measurement, ELISA or immunoblotting in cell lysates or conditioned media.
33664361	1	67	gly	N-glycosylation	144:158	arg1	proteins	proteins				Fterm		proteins			Here, we present for the first time, a site-specific N-glycosylation analysis of proteins from a Brazilian Zika virus (ZIKV) strain.
28528272	13	24	gly	unglycosylated	1935:1948	arg1	both glycosylated and unglycosylated cystatin E/M	both glycosylated and unglycosylated cystatin E/M				PUBTATOR		cystatin E/M	1474		Contrary to the importance of legumain glycosylation, both glycosylated and unglycosylated cystatin E/M showed similar capacity to inhibit legumain.
28528272	13	38	gly	glycosylated	1918:1929	arg1	both glycosylated and unglycosylated cystatin E/M	both glycosylated and unglycosylated cystatin E/M				PUBTATOR		cystatin E/M	1474		Contrary to the importance of legumain glycosylation, both glycosylated and unglycosylated cystatin E/M showed similar capacity to inhibit legumain.
30348809	5	36	gly	glycosylation	715:727	arg2	a consensus N-linked glycosylation motif			a consensus N-linked glycosylation motif						motif	Mass spectrometry revealed that the N610 residue is part of a consensus N-linked glycosylation motif in the receptor, usually linked to complex glycans.
33154157	3	56	gly	glycoproteins	591:603	arg1	ER-escaped glycoproteins	ER-escaped glycoproteins				Fterm		glycoproteins			Endo-α-1,2-mannosidase (MANEA) is the sole endo-acting glycoside hydrolase involved in N-glycan trimming and is located within the Golgi, where it allows ER-escaped glycoproteins to bypass the classical N-glycosylation trimming pathway involving ER glucosidases I and II.
31129958	2	5	gly	glycopeptides	277:289	arg2	glycopeptides			glycopeptides						glycopeptides	Many creative workflows and software apps have emerged recently that offer promising capabilities for assigning glycopeptides to MS data in an automated fashion.
33030205	3	68	gly	O-glycosylation	582:596	arg2	a site			a site						site	IMPa from Pseudomonas aeruginosa is such an O-glycopeptidase that cleaves the peptide bond immediately preceding a site of O-glycosylation, and through this glycoprotein-degrading function contributes to the host-pathogen interaction.
32282299	2	4	gly	glycoproteins	458:470	arg1	body fluid glycoproteins	body fluid glycoproteins				Fterm		glycoproteins			BACKGROUND Altered N-glycosylation of body fluid glycoproteins can be an effective indicator of most inflammatory processes.
32282299	2	35	gly	N-glycosylation	428:442	arg1	body fluid glycoproteins	body fluid glycoproteins				Fterm		glycoproteins			BACKGROUND Altered N-glycosylation of body fluid glycoproteins can be an effective indicator of most inflammatory processes.
30881698	1	38	gly	glycoproteins	117:129	arg1	glycoproteins' roles	glycoproteins' roles				Fterm		glycoproteins			As more is learned about glycoproteins' roles in human health and disease, the biological functionalities of N-linked glycans are becoming more relevant.
29980609	7	9	gly	glycosylation	1403:1415	arg1	at least one conventional glycosylation site			at least one conventional glycosylation site						site	One atypical and three conventional N-linked glycosylation sites in the AICL C-type lectin-like domain critically impact maturation and surfacing of AICL, which is strictly dependent on glycosylation of at least one conventional glycosylation site.
29980609	7	13	gly	glycosylation	1446:1458	arg2	at least one conventional glycosylation site			at least one conventional glycosylation site						site	One atypical and three conventional N-linked glycosylation sites in the AICL C-type lectin-like domain critically impact maturation and surfacing of AICL, which is strictly dependent on glycosylation of at least one conventional glycosylation site.
29980609	7	91	gly	glycosylation	1262:1274	arg2	three conventional N-linked glycosylation sites			three conventional N-linked glycosylation sites						sites	One atypical and three conventional N-linked glycosylation sites in the AICL C-type lectin-like domain critically impact maturation and surfacing of AICL, which is strictly dependent on glycosylation of at least one conventional glycosylation site.
29093093	0	89	gly	Glycoprotein	64:75	arg1	Shield	Glycoprotein			Shield	Fterm		Glycoprotein			Glycan Shield and Fusion Activation of a Deltacoronavirus Spike Glycoprotein Fine-Tuned for Enteric Infections.
30566828	12	61	gly	nonglycosylated	1441:1455	arg1	the nonglycosylated Asn-287			the nonglycosylated Asn-287				CTRP12	388581	Asn-287	Replacing the nonglycosylated Asn-287 with glutamine or alanine resulted in protein misfolding and aggregation, leading to retention in the endoplasmic reticulum.
34174425	4	100	gly	proteins	656:663	arg1	glycans	proteins			glycans	Fterm		proteins			One solution to this problem is to reprogram the glycosylation pathway of filamentous fungi to decorate proteins with glycans that match, or can be remodeled into, those that are accepted by humans.
31831633	3	29	gly	glycosylation	502:514	arg1	MUC1	MUC1				OGER		MUC1	P15941		It is not known what causes changes in glycosylation of MUC1.
33300777	3	53	gly	glycosylated	598:609	arg1	a glycosylated peptide			a glycosylated peptide						peptide	Herein, we present a new approach to efficiently screen aptamers toward specific glycans with a complex structure, using a glycosylated peptide as a scaffold.
31302509	8	30	gly	glycosylated	1190:1201	arg1	an unidentified glycosylated cofactor	an unidentified glycosylated cofactor				Fterm		cofactor			Our data also suggest that an unidentified glycosylated cofactor may influence X/P-MLV entry.
33295603	4	17	gly	proteins	752:759	arg1	repeat regions	proteins			repeat regions	Fterm		proteins			Some of these limitations stem from the difficulty to track the biosynthetic process of mucin-type O-glycosylation, especially when glycans occur in dense clusters in repeat regions of proteins, such as the mucins or immunoglobulin A1 (IgA1).
29295913	4	59	gly	mannosylation	731:743	arg1	proteins	proteins				Fterm		proteins			Histoplasma yeast cells lacking the Pmt1 and Pmt2 protein mannosyltransferases, which catalyze O-linked mannosylation of proteins, are severely attenuated during infection of mammalian hosts.
29295913	4	76	gly	proteins	748:755	arg1	O-linked mannosylation	proteins			O-linked mannosylation	Fterm		proteins			Histoplasma yeast cells lacking the Pmt1 and Pmt2 protein mannosyltransferases, which catalyze O-linked mannosylation of proteins, are severely attenuated during infection of mammalian hosts.
28871230	4	33	gly	glycoprotein	963:974	arg1	the glycoprotein glycopatterns	the glycoprotein glycopatterns				Fterm		glycoprotein			Finally, the differences of the glycoprotein glycopatterns were systematically compared between CHB and CHC patients.
31308178	1	1	gly	glycosylation	202:214	arg1	cell-surface proteins	cell-surface proteins				Fterm		proteins			The tumor microenvironment and proinflammatory signals significantly alter glycosylation of cell-surface proteins on endothelial cells.
30348809	8	58	gly	N-glycosylation	1013:1027	arg1	CSF3R	CSF3R				PUBTATOR		CSF3R	1441		Mutation of the N610 site prevented membrane-proximal N-glycosylation of CSF3R, which then drove ligand-independent cellular expansion.
34450331	12	85	gly	glycoproteins	2247:2259	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			However, the size and heterogeneity of the oligosaccharide chains poses several challenges to the applications of these techniques to glycoproteins.
34450331	12	126	gly	heterogeneity	2135:2147	arg1	the oligosaccharide chains				the oligosaccharide chains						However, the size and heterogeneity of the oligosaccharide chains poses several challenges to the applications of these techniques to glycoproteins.
31875397	9	6	gly	glycosylated	1619:1630	arg1	novel glycosylated neuropeptides			novel glycosylated neuropeptides						neuropeptides	The elucidation of novel glycosylated neuropeptides sheds light on the crustacean peptidome and offers novel insights into future neuropeptide functional studies.
27643667	3	46	gly	glycosylation	217:229	arg2	five N-linked glycosylation sites			five N-linked glycosylation sites						sites	MPO has five N-linked glycosylation sites on its heavy chains.
32858085	2	24	gly	glycoproteins	440:452	arg1	client glycoproteins	client glycoproteins				Fterm		glycoproteins			In this system, UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) plays an essential role as the "folding sensor" by virtue of its ability to discriminate folding states of client glycoproteins.
32858085	2	48	gly	glycoprotein	283:294	arg1	glycoprotein glucosyltransferase 1				glycoprotein glucosyltransferase 1						In this system, UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) plays an essential role as the "folding sensor" by virtue of its ability to discriminate folding states of client glycoproteins.
29784879	5	6	gly	glycoprotein	865:876	arg1	a misfolded glycoprotein	a misfolded glycoprotein				Fterm		glycoprotein			However, the molecular mechanism of mannose removal by EDEMs remains unclear, partly owing to the difficulty of reconstituting mannosidase activity in vitro Here, our analysis of EDEM3-mediated mannose-trimming activity on a misfolded glycoprotein revealed that ERp46, an ER-resident oxidoreductase, associates stably with EDEM3.
29784879	5	57	gly	activity	841:848	arg1	a misfolded glycoprotein	glycoprotein			activity	Fterm		glycoprotein			However, the molecular mechanism of mannose removal by EDEMs remains unclear, partly owing to the difficulty of reconstituting mannosidase activity in vitro Here, our analysis of EDEM3-mediated mannose-trimming activity on a misfolded glycoprotein revealed that ERp46, an ER-resident oxidoreductase, associates stably with EDEM3.
31931833	1	7	gly	glycoproteins	119:131	arg1	BACKGROUND Therapeutic glycoproteins	BACKGROUND Therapeutic glycoproteins				Fterm		glycoproteins			BACKGROUND Therapeutic glycoproteins have occupied an extremely important position in the market of biopharmaceuticals.
32469948	2	50	gly	glycoproteins	268:280	arg1	correct glycan structures	glycoproteins			correct glycan structures	Fterm		glycoproteins			To produce human therapeutic proteins, a host that can produce glycoproteins with correct glycan structures is required.
32699088	6	78	gly	glycosylation	1071:1083	arg2	the N-linked glycosylation sites			the N-linked glycosylation sites						sites	Analysis of IAVs carrying N1 and N2 mutants demonstrated that the N-linked glycosylation sites on the NA head domain are required for efficient virion incorporation and replication in cells and eggs.
33135055	9	25	gly	glycosylation	1445:1457	arg2	hACE2 glycosylation sites			hACE2 glycosylation sites						sites	Our elucidation of the site-specific glycosylation and its terminal orientations on the hACE2 receptor, along with the modeling of hACE2 glycosylation sites can aid in understanding the intriguing virus-receptor interactions and assist in the development of novel therapeutics to prevent viral entry.
30581149	4	3	gly	N-glycosylation	690:704	arg2	one or two N-glycosylation sites			one or two N-glycosylation sites						sites	HHM 1 and HHM 2 containing one or two N-glycosylation sites were expressed in baculovirus-infected High-Five™ insect cells and a non-glycosylated version (HHM 0) was obtained by mutating the glycosylation motif.
30581149	4	35	gly	glycosylation	843:855	arg2	the glycosylation motif			the glycosylation motif						motif	HHM 1 and HHM 2 containing one or two N-glycosylation sites were expressed in baculovirus-infected High-Five™ insect cells and a non-glycosylated version (HHM 0) was obtained by mutating the glycosylation motif.
30581149	4	85	gly	non-glycosylated	781:796	arg1	HHM 0	HHM 0				OGER		HHM	O95273		HHM 1 and HHM 2 containing one or two N-glycosylation sites were expressed in baculovirus-infected High-Five™ insect cells and a non-glycosylated version (HHM 0) was obtained by mutating the glycosylation motif.
30375453	5	59	gly	type	777:780	arg1	each site			each site	each site		Site			site	In this study, we used molecular dynamics (MD) simulation to study the force-induced unfolding of A2 domain with and without a single N-linked glycan type on each site.
33606939	0	0	gly	Shielding	15:23	arg1	Lassa Virus Envelop Protein	Protein			Shielding	Fterm		Protein			Glycan Cluster Shielding and Antibody Epitopes on Lassa Virus Envelop Protein.
33606939	0	18	gly	Epitopes	38:45	arg1	Lassa Virus Envelop Protein	Protein			Epitopes	Fterm		Protein			Glycan Cluster Shielding and Antibody Epitopes on Lassa Virus Envelop Protein.
34780171	6	6	gly	glycopeptides	1360:1372	arg2	glycopeptides			glycopeptides						glycopeptides and sialoglycopeptides	Most importantly, the ZIC-cHILIC stepwise strategy demonstrated good reproducibility (>80% in triplicate analysis) as well as superior coverage of 4.6- to 12.0-fold and 2.1- to 35.6-fold more glycopeptides and sialoglycopeptides compared to conventional TiO2 and ZIC-HILIC, respectively.
34780171	6	24	gly	sialoglycopeptides	1378:1395	arg2	sialoglycopeptides			sialoglycopeptides						glycopeptides and sialoglycopeptides	Most importantly, the ZIC-cHILIC stepwise strategy demonstrated good reproducibility (>80% in triplicate analysis) as well as superior coverage of 4.6- to 12.0-fold and 2.1- to 35.6-fold more glycopeptides and sialoglycopeptides compared to conventional TiO2 and ZIC-HILIC, respectively.
34229070	9	82	gly	O-glycopeptide	1480:1493	arg2	O-glycopeptide			O-glycopeptide						O-glycopeptide	CONCLUSION Our study established an intact O-glycopeptide MS analysis strategy for APP O-glycopeptide identification with enhanced fragmentation efficiency and detection sensitivity.
34229070	9	95	gly	O-glycopeptide	1524:1537	arg2	APP O-glycopeptide identification			APP O-glycopeptide identification						O-glycopeptide	CONCLUSION Our study established an intact O-glycopeptide MS analysis strategy for APP O-glycopeptide identification with enhanced fragmentation efficiency and detection sensitivity.
32858085	1	62	gly	glycoproteins	127:139	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			BACKGROUND In the endoplasmic reticulum (ER), folding of glycoproteins is assisted by a combined action of enzymes and chaperones that leads them to biologically functional structures.