doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_base_np	arg_protein	arg_domain	arg_site	arg_sugar	PSource	SiteSource	NProtein	NID	SiteName	sent_text
30518374	9	21	gly	sialylation	1775:1785	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.
30518374	9	85	gly	glycoproteins	1790:1802	arg1	sialylation	glycoproteins			sialylation	Fterm		glycoproteins			CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.
31987167	10	29	gly	glycoproteins	1597:1609	arg1	81 glycoproteins	81 glycoproteins				Fterm		glycoproteins			Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.
30851844	0	64	gly	N-glycopeptides	46:60	arg2	intact tryptic N-glycopeptides			intact tryptic N-glycopeptides						N-glycopeptides	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.
30940835	0	27	gly	glycopeptides	88:100	arg2	amyloid-beta glycopeptides			amyloid-beta glycopeptides						glycopeptides	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.
30172411	0	55	gly	receptor	48:55	arg1	lipopolysaccharide-induced cytokine production	toll-like receptor 4			lipopolysaccharide-induced cytokine production	OGER		toll-like receptor 4	O00206		Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.
29391424	4	2	gly	glycopeptides	538:550	arg2	O-linked glycopeptides			O-linked glycopeptides						glycopeptides	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.
29391424	4	20	gly	glycoproteins	578:590	arg1	standard glycoproteins	standard glycoproteins				Fterm		glycoproteins			Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.
31544955	1	48	gly	glycoproteins	154:166	arg1	glycoproteins	glycoproteins			N-glycans	Fterm		glycoproteins			Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.
29423317	10	56	gly	glycosylation	1671:1683	arg2	the glycosylation site sequon			site sequon						site sequon	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."
31544955	7	50	gly	contained	1035:1043	arg1	23BChE AND a slightly lower initial sialylation level	23BChE			a slightly lower initial sialylation level	PUBTATOR		BChE	590		Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.
29843473	2	12	gly	LPS	299:301	arg1	the O-polysaccharide part	LPS			the O-polysaccharide part	Cterm		LPS			O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS.
31987167	5	82	gly	glycoproteins	873:885	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.
29423317	7	80	gly	glycosylation	1255:1267	arg2	Ser			Ser						Ser	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).
29423317	7	80	gly	glycosylation	1255:1267	arg2	the glycosylation site			the glycosylation site						site	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).
31676555	1	13	gly	glycoprotein	192:203	arg1	O-linked glycoprotein carbohydrates				O-linked glycoprotein carbohydrates						The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.
31676555	1	15	gly	glycoproteins	114:126	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.
31676555	1	21	gly	derived	92:98	arg1	N-linked glycoproteins AND unconjugated oligosaccharides	N-linked glycoproteins			unconjugated oligosaccharides	Fterm		glycoproteins			The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.
30035760	2	19	gly	glycoproteins	274:286	arg1	these glycoproteins	these glycoproteins				Fterm		glycoproteins			On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.
30035760	5	20	gly	glycoproteins	761:773	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.
29393491	8	24	gly	glycosylation	1318:1330	arg1	integrin β1	integrin β1				PUBTATOR		integrin β1	3688		These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.
31659420	8	26	gly	sialylated	1745:1754	arg1	the sialylated lactulose groups				the sialylated lactulose groups						The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.
30844259	2	11	gly	residues	465:472	arg1	guluronic acid (G) residues			residues	guluronic acid (G) residues					residues	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.
30265981	2	25	gly	used	356:359	arg2	This composite			This composite						composite	This composite was then used in the electrode modification for the determination of theophylline.
31785876	2	57	gly	attachment	335:344	arg2	proteins AND polysaccharides	proteins			polysaccharides	Fterm		proteins			Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.
29524801	5	64	gly	domain	768:773	arg1	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II				the cell-wall pectic polysaccharide domain rhamnogalacturonan-II						Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.
31482162	0	45	gly	glycopeptide	21:32	arg2	Trehalose-functional glycopeptide			Trehalose-functional glycopeptide						glycopeptide	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.
29391424	6	41	gly	glycopeptide	877:888	arg2	O-linked glycopeptide spectra			O-linked glycopeptide spectra						glycopeptide	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.
29391424	6	59	gly	glycopeptides	913:925	arg2	glycopeptides			glycopeptides						glycopeptides	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.
30593802	10	37	gly	used	1473:1476	arg2	bentonite/chitosan@Co3O4 green composite			bentonite/chitosan@Co3O4 green composite						composite	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.
30851844	2	30	gly	glycoprotein	405:416	arg1	the glycoprotein	the glycoprotein				Fterm		glycoprotein			Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.
30350625	7	35	gly	N-glycopeptides	979:993	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	The de-N-deglycosylation decreased the abundance of N-glycopeptides, the desialylation simplified the O-glycans and the enzymatic oxidization conferred the enrichment specificity.
31482162	8	65	gly	glycopeptides	1317:1329	arg2	biocompatible glycopeptides			biocompatible glycopeptides						glycopeptides	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.
31482162	5	52	gly	glycopeptide	844:855	arg2	the glycopeptide			the glycopeptide						glycopeptide	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.
31248580	4	68	gly	position	599:606	arg1	glycans			position	glycans					position	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.
31217009	5	7	gly	used	1000:1003	arg2	the composites			the composites						composites	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.
30903686	3	38	gly	glycosylation	608:620	arg2	human and mouse glycosylation sites			human and mouse glycosylation sites						sites	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.
30903686	3	50	gly	glycosylation	729:741	arg2	N-/O-linked glycosylation sites			N-/O-linked glycosylation sites						sites	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.
30635335	3	19	gly	pole	590:593	arg1	the expected growth sites			the expected growth sites						sites	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.
31987167	1	59	gly	glycoproteins	167:179	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions.
30035760	6	17	gly	glycoproteins	852:864	arg1	These recombinant glycoproteins	These recombinant glycoproteins				Fterm		glycoproteins			These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.
31659420	5	1	gly	sialylated	988:997	arg1	the sialylated lactulose groups				the sialylated lactulose groups						At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.
29760197	6	73	gly	GIPC	1140:1143	arg1	GlcN(Ac) containing GIPCs	GIPC			GlcN(Ac) containing GIPCs	OGER		GIPC	O14908		However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.
30210478	1	11	gly	linked	326:331	arg1	other proteins AND a unique, complex decasaccharide	other proteins			a unique, complex decasaccharide	Fterm		proteins			The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.
31079452	4	36	gly	glycoprotein	885:896	arg1	a model glycoprotein	a model glycoprotein				Fterm		glycoprotein			Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.
31079452	4	36	gly	glycoprotein	885:896	arg1	the AXL receptor tyrosine kinase (AXL)	the AXL receptor tyrosine kinase (AXL)				PUBTATOR		AXL	558		Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.
31079452	4	67	gly	N-glycosylation	912:926	arg2	multiple N-glycosylation sites			multiple N-glycosylation sites						sites	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.
31079452	4	93	gly	N-glycopeptides	1083:1097	arg2	N-glycopeptides			N-glycopeptides						N-glycopeptides	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.
31677578	2	73	gly	occupied	378:385	arg2	the adsorptive sites			the adsorptive sites						sites	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.
31565939	6	25	gly	residue	1083:1089	arg1	the hexasaccharide analogue			residue in	the hexasaccharide analogue					residue in	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.
31544955	4	34	gly	26BChE	605:610	arg1	The N-glycan composition	BChE			The N-glycan composition	PUBTATOR		BChE	100767789		The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.
31539460	2	31	gly	enzymes	412:418	arg1	soluble saccharides	enzymes			soluble saccharides	Fterm		enzymes			This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.
31659420	7	7	gly	sialylated	1413:1422	arg1	the sialylated lactulose groups				the sialylated lactulose groups						Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.
30614079	5	60	gly	acid	912:915	arg1	residues			residues						alanine residues	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.
31079452	2	43	gly	peptide	553:559	arg1	unexpected glycan or peptide sequences				unexpected glycan or peptide sequences						Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.
31853645	16	55	gly	used	1849:1852	arg2	The nanocomposite			The nanocomposite						nanocomposite	The nanocomposite was used to construct a chemiluminescence (CL) assay for tetracycline.
30728244	3	93	gly	terminus	564:571	arg1	the first mannose			terminus	the first mannose					terminus	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.
31079452	5	66	gly	unglycosylated	1179:1192	arg1	unglycosylated peptides			unglycosylated peptides						peptides	Conversely, unglycosylated peptides did not exhibit this grouping behavior.
30940587	10	64	gly	utilized	1407:1414	arg2	The synthesized n-GO@HTCS biocomposite			The synthesized n-GO@HTCS biocomposite						biocomposite	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.
31434476	1	94	gly	origin	222:227	arg1	a polysaccharide	origin			a polysaccharide	Fterm		origin			Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.
30851844	4	8	gly	N-glycopeptide	679:692	arg2	a practical intact tryptic N-glycopeptide enrichment method			a practical intact tryptic N-glycopeptide enrichment method						N-glycopeptide	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.
30851844	4	10	gly	N-glycopeptides	918:932	arg2	enrich intact N-glycopeptides			enrich intact N-glycopeptides						N-glycopeptides	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.
29055059	10	101	gly	glycopeptides	1804:1816	arg2	O-linked glycopeptides			O-linked glycopeptides						glycopeptides	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.
29558455	3	4	gly	glycoproteins	470:482	arg1	S-layer glycoproteins	S-layer glycoproteins				Fterm		glycoproteins			Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.
31107901	5	59	gly	glycans	1677:1683	arg1	A solvent-exposed peptide			A solvent-exposed peptide	A solvent-exposed peptide		Site			peptide	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.
31107901	5	59	gly	glycans	1677:1683	arg1	smugS peptide			smugS peptide	smugS peptide		Site			peptide	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.
31107901	5	137	gly	trisaccharide	1615:1627	arg1	A solvent-exposed peptide			A solvent-exposed peptide	A solvent-exposed peptide		Site			peptide	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.
31107901	5	137	gly	trisaccharide	1615:1627	arg1	smugS peptide			smugS peptide	smugS peptide		Site			peptide	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.
31207953	4	97	gly	moieties	969:976	arg1	position			position	position		Site			position	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.
31207953	4	110	gly	position	903:910	arg1	linear tetrasaccharide carbohydrate moieties			position	linear tetrasaccharide carbohydrate moieties					position	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.
31195757	6	52	gly	containing	791:800	arg1	HMOs AND α-1,2-linked fucose	HMOs			α-1,2-linked fucose	OGER		However, HMOs	P00540		However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.
30690438	9	43	gly	used	1697:1700	arg2	the mHA@Chi-g-PMMA composite			the mHA@Chi-g-PMMA composite						composite	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.
30690438	9	65	gly	utilized	1638:1645	arg2	the mHA@Chi-g-PMMA composite			the mHA@Chi-g-PMMA composite						composite	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.
30518374	2	41	gly	sialylation	508:518	arg1	cerebral glycoproteins	cerebral glycoproteins				Fterm		glycoproteins			In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.
30518374	2	76	gly	glycoproteins	532:544	arg1	sialylation	glycoproteins			sialylation	Fterm		glycoproteins			In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.
31544955	2	47	gly	glycoproteins	237:249	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Human cells produce glycoproteins that include α-2,3 and α-2,6 sialic acids.
31332537	4	23	gly	α-chitin	631:638	arg1	the GlcNAc bioproduction	α-chitin			the GlcNAc bioproduction	Fterm		α-chitin			In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).
29215790	1	61	gly	fucosylated	191:201	arg1	sialyl-Lewis X/A				sialyl-Lewis X/A						Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.
29215790	1	61	gly	fucosylated	191:201	arg1	fucosylated glycans				fucosylated glycans						Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.
31248580	3	86	gly	attached	509:516	arg1	membrane proteins AND glycan chains	membrane proteins			glycan chains	Fterm		proteins			Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.
35021376	6	45	gly	glycoprotein	897:908	arg1	The optimized glycoprotein microarray platform	The optimized glycoprotein microarray platform				Fterm		glycoprotein			The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.
35021376	6	70	gly	glycoproteins	980:992	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.
30903686	2	3	gly	glycosylation	456:468	arg2	glycosylation sites			glycosylation sites						sites	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.
30903686	2	11	gly	glycopeptides	430:442	arg2	glycopeptides			glycopeptides						glycopeptides	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.
30903686	2	16	gly	microheterogeneity	408:425	arg1	glycopeptides			glycopeptides						glycopeptides	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.
31853645	8	25	gly	used	1060:1063	arg2	The composite			The composite						composite	The composite was used to construct a CL assay for tetracycline.
31284011	1	25	gly	used	246:249	arg2	A magnetic chitosan-based biocomposite			A magnetic chitosan-based biocomposite						biocomposite	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.
35021376	3	75	gly	glycoproteins	338:350	arg1	glycan patterns	glycoproteins			glycan patterns	Fterm		glycoproteins			Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.
30794378	4	4	gly	glycosylated	713:724	arg1	glycosylated peptides			glycosylated peptides						peptides	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.
30035760	10	64	gly	glycoproteins	1568:1580	arg1	mammalian glycoproteins	mammalian glycoproteins				Fterm		glycoproteins			The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.
29941663	6	24	gly	glycoprotein	900:911	arg1	P-selectin glycoprotein ligand-1	P-selectin glycoprotein ligand-1				PUBTATOR		P-selectin glycoprotein ligand-1	6404		E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.
29941663	6	71	gly	glycoforms	875:884	arg1	i.e., cutaneous lymphocyte Ag [CLA	i.e., cutaneous lymphocyte Ag [CLA				PUBTATOR		Ag [CLA	6404		E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.
29941663	6	71	gly	glycoforms	875:884	arg1	E-selectin binding glycoforms	E-selectin binding glycoforms				PUBTATOR		E-selectin	6401		E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.
29941663	6	71	gly	glycoforms	875:884	arg1	P-selectin glycoprotein ligand-1	P-selectin glycoprotein ligand-1				PUBTATOR		P-selectin glycoprotein ligand-1	6404		E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.
29941663	6	71	gly	glycoforms	875:884	arg1	CD44	CD44				PUBTATOR		CD44	960		E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.
29941663	6	71	gly	glycoforms	875:884	arg1	PSGL-1	PSGL-1				OGER		PSGL-1	Q14242		E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.
29391424	2	51	gly	glycopeptide	239:250	arg2	intact glycopeptide mass spectra			intact glycopeptide mass spectra						glycopeptide	We present an approach for automated annotation of intact glycopeptide mass spectra.
31334644	2	6	gly	sialylated	427:436	arg1	sialylated oligosaccharides	HMOs			sialylated oligosaccharides	OGER		HMOs			However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.
30035760	7	51	gly	glycoprotein	1042:1053	arg1	glycoprotein binding	glycoprotein binding				Fterm		glycoprotein			We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.
29995974	0	62	gly	residues	13:20	arg1	the lipopolysaccharide exporter			residues in	the lipopolysaccharide exporter					residues in	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.
31482162	7	20	gly	glycopeptide	1253:1264	arg2	glycopeptide			glycopeptide						glycopeptide	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.
30794378	9	47	gly	glycopeptides	1647:1659	arg2	glycopeptides enrichment			glycopeptides enrichment						glycopeptides	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.
31544955	5	37	gly	di-sialylated	787:799	arg1	bi-antennary di-sialylated glycans				bi-antennary di-sialylated glycans						Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.
31147012	2	51	gly	used	421:424	arg2	pulse electrochemical deposition			pulse electrochemical deposition						position	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.
31079452	1	3	gly	N-glycosylated	186:199	arg1	N-glycosylated peptides			N-glycosylated peptides						peptides	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.
31079452	1	84	gly	peptide	389:395	arg1	possible glycan and peptide combinations				possible glycan and peptide combinations						The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.
30350625	9	3	gly	glycoproteins	1360:1372	arg1	38 glycoproteins	38 glycoproteins				Fterm		glycoproteins			This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.
30350625	9	62	gly	peptide	1322:1328	arg1	59 O-GalNAc modified peptide sequences				59 O-GalNAc modified peptide sequences						This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.
30035760	8	21	gly	glycoproteins	1145:1157	arg1	Recombinant glycoproteins	Recombinant glycoproteins				Fterm		glycoproteins			Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.
29055059	13	31	gly	glycosylation	2286:2298	arg1	a therapeutic protein	a therapeutic protein				Fterm		protein			This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.
29055059	8	56	gly	glycopeptides	1395:1407	arg2	glycopeptides			glycopeptides						glycopeptides	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.
31107901	3	113	gly	glycoproteins	506:518	arg1	the major surface glycoproteins	the major surface glycoproteins				Fterm		glycoproteins	18972		METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.
31255519	0	8	gly	Impact	0:5	arg1	glucose homeostasis	Impact			glucose homeostasis	OGER		Impact	O55091		Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.
30560095	0	40	gly	Domain	14:19	arg1	Two Domain, Cell Wall				Two Domain, Cell Wall						Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.
30518374	7	24	gly	glycoproteins	1273:1285	arg1	brain glycoproteins	brain glycoproteins				Fterm		glycoproteins			At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.
30518374	7	99	gly	sialylation	1239:1249	arg1	brain glycoproteins	glycoproteins			sialylation	Fterm		glycoproteins			At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.
30518374	7	99	gly	sialylation	1239:1249	arg1	N-glycans	glycoproteins			N-glycans	Fterm		glycoproteins			At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.
30851844	5	25	gly	N-glycopeptides	1008:1022	arg2	the intact N-glycopeptides analysis			the intact N-glycopeptides analysis						N-glycopeptides	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.
31312254	7	56	gly	N-glycosylation	1225:1239	arg1	the rhLF	rhLF		sites		OGER		rhLF	P02788	sites	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.
29391424	5	25	gly	glycosylation	668:680	arg2	970 unique glycosylation sites			970 unique glycosylation sites						sites	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.
29391424	5	42	gly	glycoproteins	632:644	arg1	257 glycoproteins	257 glycoproteins				Fterm		glycoproteins			In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.
29391424	5	50	gly	glycopeptide	720:731	arg2	3447 non-redundant N-linked glycopeptide variants			3447 non-redundant N-linked glycopeptide variants						glycopeptide	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.
30593802	8	10	gly	used	1181:1184	arg2	The composite			The composite						composite	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.
30210478	2	111	gly	glycoproteins	420:432	arg1	The S-layer glycoproteins	The S-layer glycoproteins				Fterm		glycoproteins			The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.
30210478	2	111	gly	glycoproteins	420:432	arg1	virulence factors	virulence factors				Fterm		factors			The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.
30692173	9	93	gly	sialylated	1643:1652	arg1	sialylated lipooligosaccharide				sialylated lipooligosaccharide						C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.
31676555	3	23	gly	glycoproteins	527:539	arg1	O-mannosylated glycoproteins	O-mannosylated glycoproteins				Fterm		glycoproteins			These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.
31676555	3	24	gly	O-mannosylated	512:525	arg1	O-mannosylated glycoproteins	O-mannosylated glycoproteins				Fterm		glycoproteins			These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.
31945552	3	81	gly	used	684:687	arg2	The stable hemin bio-composites			The stable hemin bio-composites						bio-composites	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.
30794378	5	29	gly	glycopeptides	748:760	arg2	Thirty-four glycopeptides			Thirty-four glycopeptides						glycopeptides	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.
30407903	5	8	gly	C16 	801:804	arg1	C18 			C18 				nov	P48745	C18 	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).
30407903	5	8	gly	C16 	801:804	arg1	C16 			C16 				nov	P48745	C16 	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).
30424006	7	12	gly	metabolism	1217:1226	arg1	the mushroom-fed pigs	pigs			metabolism	OGER		pigs	Q96S52		LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.
30424006	7	54	gly	pigs	1290:1293	arg1	bacterial carbohydrate metabolism	pigs			bacterial carbohydrate metabolism	OGER		pigs	Q96S52		LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.
30242779	1	58	gly	composition	235:245	arg1	prolific D'man sheep			position	prolific D'man sheep					position	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.
30350625	1	11	gly	glycoproteins	185:197	arg1	various N-linked and O-linked glycoproteins	various N-linked and O-linked glycoproteins				Fterm		glycoproteins			Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.
29391424	0	9	gly	glycopeptide	19:30	arg2	Large-scale intact glycopeptide identification			Large-scale intact glycopeptide identification						glycopeptide	Large-scale intact glycopeptide identification by Mascot database search.
29391424	3	32	gly	glycoprotein	469:480	arg1	custom glycoprotein databases	custom glycoprotein databases				Fterm		glycoprotein			The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.
29391424	3	65	gly	glycopeptide	324:335	arg2	intact glycopeptide analysis			intact glycopeptide analysis						glycopeptide	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.
29719608	6	26	gly	N-glycosylation	862:876	arg1	CD147	CD147				PUBTATOR		CD147	682		Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.
31195757	7	58	gly	sialylated	939:948	arg1	sialylated oligosaccharides				sialylated oligosaccharides						The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.
30797481	11	30	gly	sites	1288:1292	arg1	Bman26			sites	Bman26					sites	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.
30903686	0	62	gly	N-	23:24	arg1	sites			sites						sites	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.
30903686	0	73	gly	glycosylation	39:51	arg1	human and mouse proteins	human and mouse proteins				Fterm		proteins			SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.
30851844	6	14	gly	glycosylation	1283:1295	arg2	261 glycosylation sites			261 glycosylation sites						sites	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.
30851844	6	18	gly	glycoproteins	1310:1322	arg1	191 glycoproteins	191 glycoproteins				Fterm		glycoproteins			Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.
30851844	6	29	gly	N-glycopeptides	1246:1260	arg2	700 site-specific intact tryptic N-glycopeptides			700 site-specific intact tryptic N-glycopeptides						N-glycopeptides	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.
31482162	2	55	gly	glycopeptides	357:369	arg2	glycopeptides			glycopeptides						glycopeptides	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.
30518374	10	58	gly	sialoglycoproteins	1862:1879	arg1	sialoglycoproteins	sialoglycoproteins				Fterm		sialoglycoproteins			Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.
30518374	0	59	gly	sialylation	24:34	arg1	cerebral glycoproteins	cerebral glycoproteins				Fterm		glycoproteins			Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.
30518374	0	104	gly	glycoproteins	48:60	arg1	cerebral glycoproteins	glycoproteins			sialylation	Fterm		glycoproteins			Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.
30350625	2	31	gly	glycoproteins	257:269	arg1	the serum O-linked glycoproteins	the serum O-linked glycoproteins				Fterm		glycoproteins			Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.
30350625	2	63	gly	glycoproteins	223:235	arg1	N-linked glycoproteins	N-linked glycoproteins				Fterm		glycoproteins			Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.
30796749	10	91	gly	SG0102T	1357:1363	arg1	The cell wall peptidoglycan	SG0102T			The cell wall peptidoglycan	Cterm		SG0102T			The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.
31987167	8	50	gly	glycoproteins	1316:1328	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum.
30940835	3	32	gly	glycopeptide	672:683	arg2	an isotopically double-labeled Aβ1-15 glycopeptide			an isotopically double-labeled Aβ1-15 glycopeptide						glycopeptide	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30940835	3	57	gly	structure	799:807	arg1	human CSF	CSF			structure	OGER		CSF			We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30940835	3	63	gly	glycopeptides	1029:1041	arg2	these Aβ glycopeptides			these Aβ glycopeptides						glycopeptides	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30940835	3	79	gly	desialylated	831:842	arg1	immunopurified and desialylated Aβ glycopeptides			immunopurified and desialylated Aβ glycopeptides						glycopeptides	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30940835	3	101	gly	glycopeptides	965:977	arg2	desialylated Aβ1-15 (and Aβ1-17) glycopeptides			desialylated Aβ1-15 (and Aβ1-17) glycopeptides						glycopeptides	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30940835	3	93	gly	carrying	686:693	arg1	an isotopically double-labeled Aβ1-15 glycopeptide AND the core 1 Galβ3GalNAcα1-O-Tyr-10 structure			an isotopically double-labeled Aβ1-15 glycopeptide	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure					glycopeptide	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30940835	3	88	gly	glycopeptides	847:859	arg1	the definite glycan core 1 structure			glycopeptides	the definite glycan core 1 structure					glycopeptides	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.
30035760	4	16	gly	glycoprotein	616:627	arg1	mammalian glycoprotein ectodomains	mammalian glycoprotein ectodomains				Fterm		glycoprotein			We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.
30903686	5	78	gly	glycoproteins	1013:1025	arg1	human glycoproteins	human glycoproteins				Fterm		glycoproteins			For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.
30035760	9	67	gly	glycoprotein	1346:1357	arg1	glycoprotein crystals	glycoprotein crystals				Fterm		glycoprotein			We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.
30518374	4	88	gly	sialylation	818:828	arg1	cerebral glycoproteins	cerebral glycoproteins				Fterm		glycoproteins			Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.
30518374	4	8	gly	glycoproteins	842:854	arg1	cerebral glycoproteins	glycoproteins			sialylation	Fterm		glycoproteins			Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.
30794378	7	31	gly	glycopeptides	1315:1327	arg2	glycopeptides			glycopeptides						glycopeptides	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
30794378	7	33	gly	N-glycosylation	1216:1230	arg2	157 N-glycosylation sites			157 N-glycosylation sites						sites	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
30794378	7	41	gly	glycoproteins	1077:1089	arg1	56 glycoproteins	56 glycoproteins				Fterm		glycoproteins			Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
30794378	7	64	gly	glycopeptides	1047:1059	arg2	76 glycopeptides			76 glycopeptides						glycopeptides	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
30794378	7	71	gly	glycoproteins	1193:1205	arg1	129 glycoproteins	129 glycoproteins				Fterm		glycoproteins			Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
30794378	7	78	gly	N-glycosylation	1099:1113	arg2	83 N-glycosylation sites			83 N-glycosylation sites						sites	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
30794378	7	84	gly	glycopeptides	1162:1174	arg2	149 glycopeptides			149 glycopeptides						glycopeptides	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.
31857120	6	15	gly	found	1468:1472	arg1	CSF AND short carbohydrate chains	CSF			short carbohydrate chains	OGER		CSF			By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.
30794378	1	60	gly	glycopeptides	174:186	arg2	glycopeptides enrichment efficiency			glycopeptides enrichment efficiency						glycopeptides	A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency.
29520318	1	44	gly	sialylated	137:146	arg1	sialylated glycans				sialylated glycans						Influenza virus infects cells by binding to sialylated glycans on the cell surface.
30794378	8	83	gly	glycopeptides	1459:1471	arg2	glycopeptides			glycopeptides						glycopeptides	In addition, GO-PEI-Carr exhibited a unique repeatability and stability even after enrichment of glycopeptides for 20 times.
31491490	10	5	gly	group	1385:1389	arg1	glutamine			glutamine						glutamine	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).
31546409	6	15	gly	utilized	1405:1412	arg2	the fabricated chitosan-coated porous PCL/BG nanocomposite			the fabricated chitosan-coated porous PCL/BG nanocomposite						nanocomposite	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.
30940835	2	43	gly	sialylated	419:428	arg1	sialylated Tyr-10 O-glycosylated Aβ peptides			sialylated Tyr-10 O-glycosylated Aβ peptides						peptides	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.
30348015	9	34	gly	contained	1614:1622	arg1	an ECM AND glycosaminoglycans	an ECM			glycosaminoglycans	OGER		ECM	Q13201		Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans.
31079452	6	14	gly	N-glycopeptides	1394:1408	arg2	the N-glycopeptides			the N-glycopeptides						N-glycopeptides	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.
31079452	6	74	gly	peptide	1291:1297	arg1	the glycan and peptide composition				the glycan and peptide composition						Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.
31079452	6	18	gly	N-glycopeptide	1323:1336	arg1	the glycan and peptide composition			N-glycopeptide	the glycan and peptide composition					N-glycopeptide	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.
29763698	2	56	gly	used	335:338	arg2	The resulting alginate-CuxZn1-xO composites			The resulting alginate-CuxZn1-xO composites						composites	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.
30382612	2	75	gly	glycosylated	333:344	arg1	highly glycosylated proteins	highly glycosylated proteins				Fterm		proteins			They produce mucins, highly glycosylated proteins.
32255017	4	81	gly	utilized	726:733	arg2	The obtained CDs-3D-PG-Pd@Au NCs composite			The obtained CDs-3D-PG-Pd@Au NCs composite						composite	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.
30560095	8	44	gly	domains	1027:1033	arg1	Peptidoglycan binding activity				Peptidoglycan binding activity						Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.
30560095	8	90	gly	Rv1288	1012:1017	arg1	Peptidoglycan binding activity	Rv1288			Peptidoglycan binding activity	PUBTATOR		Rv1288	886974		Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.
31482162	4	53	gly	glycopeptide	618:629	arg2	The specifically synthesized glycopeptide			The specifically synthesized glycopeptide						glycopeptide	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.
31212920	1	50	gly	present	210:216	arg1	human milk AND Human milk oligosaccharides	human milk			Human milk oligosaccharides	PUBTATOR		milk	100532204		Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.
31212920	1	50	gly	present	210:216	arg2	human milk AND multifunctional carbohydrates	human milk			multifunctional carbohydrates	PUBTATOR		milk	100532204		Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.
31987167	4	33	gly	glycoproteins	719:731	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).
30899062	4	65	gly	fourth	609:614	arg1	glycines			glycines						glycines	FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively.
31079452	8	29	gly	N-glycopeptides	1647:1661	arg2	more than 500 unique N-glycopeptides			more than 500 unique N-glycopeptides						N-glycopeptides	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.
31987167	0	26	gly	glycoproteins	75:87	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.
29352487	0	26	gly	isoform	89:95	arg1	Disaccharide combinations	isoform			Disaccharide combinations	Fterm		isoform			Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.
30851844	3	0	gly	glycopeptides	602:614	arg2	lowly abundant glycopeptides			lowly abundant glycopeptides						glycopeptides	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.
30851844	3	5	gly	non-glycosylated	528:543	arg1	highly abundant non-glycosylated peptides			highly abundant non-glycosylated peptides						peptides	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.
30851844	3	7	gly	N-glycopeptide	435:448	arg2	intact N-glycopeptide analysis			intact N-glycopeptide analysis						N-glycopeptide	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.
29391424	1	7	gly	glycopeptides	107:119	arg2	glycopeptides			glycopeptides						glycopeptides	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.
30064105	1	18	gly	used	190:193	arg2	The ligno-cellulose residues			The ligno-cellulose residues						residues	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.
30940835	4	59	gly	glycopeptides	1349:1361	arg2	the desialylated Aβ1-15 or Aβ1-17 glycopeptides			the desialylated Aβ1-15 or Aβ1-17 glycopeptides						glycopeptides	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.
30940835	4	75	gly	glycopeptides	1189:1201	arg2	the glycopeptides			glycopeptides	core 1 structures					glycopeptides	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.
30940835	5	5	gly	glycopeptide	1473:1484	arg2	double-labeled glycopeptide standards			double-labeled glycopeptide standards						glycopeptide	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.
30940835	5	24	gly	sialylation	1647:1657	arg1	individual Aβ glycopeptides			individual Aβ glycopeptides						glycopeptides	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.
30940835	5	72	gly	glycopeptides	1543:1555	arg2	glycopeptides			glycopeptides						glycopeptides	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.
30940835	5	92	gly	glycopeptides	1685:1697	arg2	individual Aβ glycopeptides			glycopeptides	specific sialylation patterns					glycopeptides	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.
31207953	6	39	gly	has	1145:1147	arg1	Psolusoside I AND an unprecedented branched tetrasaccharide disulfated carbohydrate moiety			I (7)	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety			H1 (6)	3010	I (7)	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.
29215790	4	93	gly	fucosylated	573:583	arg1	the fucosylated structures				the fucosylated structures						Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.
35021376	4	21	gly	glycoproteins	501:513	arg1	glycoproteins	glycoproteins				Fterm		glycoproteins			In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.
35021376	4	34	gly	nonglycosylated	596:610	arg1	high-abundant nonglycosylated proteins	high-abundant nonglycosylated proteins				Fterm		proteins			In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.
29055059	4	77	gly	glycopeptides	750:762	arg2	glycopeptides			glycopeptides						glycopeptides	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.