doc_id sent_index relation_id relation trigger trigger_offset arg_num arg_base_np arg_protein arg_domain arg_site arg_sugar PSource SiteSource NProtein NID SiteName sent_text 8286855 4 7 part_of Ser23 661:665 arg1 GpA GpA Ser23 PUBTATOR AminoAcid GpA 2993 Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 Carbohydrate was absent on Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 in GpA. 8286855 4 13 part_of Ser15 654:658 arg1 GpA GpA Ser15 PUBTATOR AminoAcid GpA 2993 Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 Carbohydrate was absent on Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 in GpA. 8286855 4 18 part_of Ser14 647:651 arg1 GpA GpA Ser14 PUBTATOR AminoAcid GpA 2993 Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 Carbohydrate was absent on Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 in GpA. 8286855 4 25 part_of Ser1 641:644 arg1 GpA GpA Ser1 PUBTATOR AminoAcid GpA 2993 Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 Carbohydrate was absent on Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 in GpA. 8286855 4 63 part_of Thr58 678:682 arg1 GpA GpA Thr58 PUBTATOR AminoAcid GpA 2993 Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 Carbohydrate was absent on Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 in GpA. 8286855 4 69 part_of Thr28 668:672 arg1 GpA GpA Thr28 PUBTATOR AminoAcid GpA 2993 Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 Carbohydrate was absent on Ser1, Ser14, Ser15, Ser23, Thr28 and Thr58 in GpA. 11710528 1 23 gly cells--A431 309:319 arg1 hTR hTR cells--A431 PUBTATOR hTR P25116 The structural analysis of high mannose-type Asn-linked (N-linked) oligosaccharides of the human transferrin receptor (hTR) from D-[2-3H]mannose metabolic-radiolabeled human cells--A431, K562, BeWo, and HL60--was investigated. 11710528 1 23 gly cells--A431 309:319 arg1 the human transferrin receptor transferrin receptor cells--A431 PUBTATOR transferrin receptor 7037 The structural analysis of high mannose-type Asn-linked (N-linked) oligosaccharides of the human transferrin receptor (hTR) from D-[2-3H]mannose metabolic-radiolabeled human cells--A431, K562, BeWo, and HL60--was investigated. 11710528 1 41 gly receptor 244:251 arg1 high mannose-type Asn-linked (N-linked) oligosaccharides transferrin receptor high mannose-type Asn-linked (N-linked) oligosaccharides PUBTATOR transferrin receptor 7037 The structural analysis of high mannose-type Asn-linked (N-linked) oligosaccharides of the human transferrin receptor (hTR) from D-[2-3H]mannose metabolic-radiolabeled human cells--A431, K562, BeWo, and HL60--was investigated. 2498325 0 83 gly Glycosylation 0:12 arg1 human apolipoprotein E human apolipoprotein E PUBTATOR apolipoprotein E 348 Glycosylation of human apolipoprotein E. 19202066 3 38 gly modified 530:537 arg3 IKKbeta AND O-linked beta-N-acetyl glucosamine IKKbeta O-linked beta-N-acetyl glucosamine PUBTATOR IKKbeta 16150 Here, we demonstrate that IKKbeta, a component of the IKK complex, was constitutively modified with O-linked beta-N-acetyl glucosamine (O-GlcNAc) in both p53-deficient mouse embryonic fibroblasts (MEFs) and transformed human fibroblasts. 19202066 3 38 gly modified 530:537 arg3 IKKbeta AND O-GlcNAc IKKbeta O-GlcNAc PUBTATOR IKKbeta 16150 Here, we demonstrate that IKKbeta, a component of the IKK complex, was constitutively modified with O-linked beta-N-acetyl glucosamine (O-GlcNAc) in both p53-deficient mouse embryonic fibroblasts (MEFs) and transformed human fibroblasts. 32155444 4 31 gly glycoprotein 519:530 arg1 the SARS-CoV-2 S glycoprotein the SARS-CoV-2 S glycoprotein Cterm S glycoprotein 43740568 We found that the SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs. 8576151 5 63 gly glycosylated 636:647 arg1 The purified procollagenase-3 The purified procollagenase-3 PUBTATOR collagenase-3 4322 The purified procollagenase-3 was shown to be glycosylated and displayed a M(r) of 60,000, the N-terminal sequence being LPLPSGGD, which is consistent with the cDNA-predicted sequence. 24308486 2 47 gly glycosylation 364:376 arg1 IgE IgE PUBTATOR IgE P01854 Here, we present site-specific glycosylation analysis of IgE from three different sources: IgE from the serum of a hyperimmune donor, from the pooled serum of multiple nondiseased donors, and from the pooled serum of 2 patients with IgE myeloma. 20133774 4 50 gly CNTN4 826:830 arg1 the four N-terminal immunoglobulin repeats CNTN4 the four N-terminal immunoglobulin repeats PUBTATOR CNTN4 269784 Furthermore, we present crystal structures of the four N-terminal immunoglobulin repeats of mouse CNTN4 both alone and in complex with the carbonic anhydrase-like domain of mouse PTPRG. 25092234 3 78 gly glycosylation 462:474 arg1 hFXI hFXI PUBTATOR hFXI 2160 This study reports the first in-depth glycosylation analysis of hFXI based on advanced MS approaches. 7107587 13 31 gly contains 1613:1620 arg1 fibrinogen AND four oligosaccharide chains fibrinogen four oligosaccharide chains PUBTATOR fibrinogen 2244 Based on carbohydrate composition, 1H-NMR spectroscopy, sequential exoglycosidase digestion, and gas chromatography-mass spectrometry of derived partially methylated alditol acetates, we propose that fibrinogen contains four oligosaccharide chains of the structure shown below. 19038967 4 63 gly glycosylated 872:883 arg1 fully glycosylated TPP1 fully glycosylated TPP1 Cterm TPP1 In this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing four Asn-linked N-acetylglucosamines that is indistinguishable from fully glycosylated TPP1 in terms of autocatalytic processing of the proform and enzymatic properties of the mature protease. 19038967 4 37 gly H-deglycosylated 757:772 arg1 an endoglycosidase H-deglycosylated form H-deglycosylated form of TPP1 Cterm H-deglycosylated form of TPP1 In this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing four Asn-linked N-acetylglucosamines that is indistinguishable from fully glycosylated TPP1 in terms of autocatalytic processing of the proform and enzymatic properties of the mature protease. 7514386 4 95 gly form 511:514 arg1 the asparagine-linked sugar chain form of CD59 the asparagine-linked sugar chain PUBTATOR form of CD59 966 The structures of the GPI anchor and the asparagine-linked sugar chain of a soluble form of CD59 in urine, U-CD59, were determined. 16770729 0 31 gly Glycosylation 0:12 arg1 Kv1.2 potassium channel Kv1.2 potassium channel OGER potassium channel Glycosylation and cell surface expression of Kv1.2 potassium channel are regulated by determinants in the pore region. 2943741 1 55 gly leukosialin 178:188 arg1 O-linked oligosaccharides leukosialin O-linked oligosaccharides PUBTATOR leukosialin P16150 Structures of O-linked oligosaccharides of leukosialin isolated from K562 erythroid, HL-60 promyelocytic, and HSB-2 T-lymphoid cell lines were examined. 16877748 8 47 gly N-glycosylation 1406:1420 arg1 mouse sICAM-1 mouse sICAM-1 Cterm sICAM-1 3383 The N-glycosylation sites of mouse sICAM-1 were analyzed by MALDI-Fourier transform ion cyclotron resonance (FTICR)-MS and nanoLC-ESI-FTICR-MS of tryptic digests of mouse sICAM-1 expressed in the Lec1 mutant of CHO cells. 7654718 1 30 gly characteristics 133:147 arg1 C-tail C-tail characteristics Cterm C-tail Glycosylation positions and oligosaccharide characteristics in the proline-rich, mucin-like, C-terminal region (C-tail) of human milk bile salt-activated lipase (BAL) were studied in order to assess the possible physiological functions of this region. 10024660 1 17 gly derived 165:171 arg2 bile salt-stimulated lipase AND N- glycans bile salt-stimulated lipase N- glycans PUBTATOR bile salt-stimulated lipase P19835 The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. 10024660 1 17 gly derived 165:171 arg1 BSSL AND N- glycans BSSL N- glycans PUBTATOR BSSL P19835 The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. 9334252 0 40 gly RNase 25:29 arg1 C-Mannosylation RNase 2 C-Mannosylation PUBTATOR RNase 2 6036 C-Mannosylation of human RNase 2 is an intracellular process performed by a variety of cultured cells. 19951703 9 12 gly glycosylation 1240:1252 arg1 UGT1A9 UGT1A9 PUBTATOR UGT1A9 54600 These results suggest that the glycosylation that occurs during translation is important for the folding of UGT1A9. 10397151 10 55 gly RIIa 1294:1297 arg1 core mannose oligosaccharide side chains Fc gamma RIIa core mannose oligosaccharide side chains PUBTATOR Fc gamma RIIa 2212 Electrospray ionisation mass spectrometry (ESMS) indicate that the N-glycans of baculovirus derived Fc gamma RIIa are core mannose oligosaccharide side chains. 10397151 10 55 gly RIIa 1294:1297 arg1 the N-glycans Fc gamma RIIa the N-glycans PUBTATOR Fc gamma RIIa 2212 Electrospray ionisation mass spectrometry (ESMS) indicate that the N-glycans of baculovirus derived Fc gamma RIIa are core mannose oligosaccharide side chains. 2033371 7 116 gly carry 1216:1220 arg1 WAS lymphocyte CD43 AND predominantly the branched structure NeuNAc alpha 2----3Gal beta 1----3 (NeuNAc alpha 2----3Gal beta 1----4G1cNAc beta 1----6) GalNAcOH WAS lymphocyte CD43 predominantly the branched structure NeuNAc alpha 2----3Gal beta 1----3 (NeuNAc alpha 2----3Gal beta 1----4G1cNAc beta 1----6) GalNAcOH PUBTATOR CD43 6693 WAS lymphocyte CD43 was found to carry predominantly the branched structure NeuNAc alpha 2----3Gal beta 1----3 (NeuNAc alpha 2----3Gal beta 1----4G1cNAc beta 1----6) GalNAcOH whereas normal lymphocytes carry the structure NeuNAc alpha 2----3Gal beta 1----3 (NeuNAc alpha 2----6) GalNAcOH. 19690161 6 23 gly glycosylation 736:748 arg1 functional vIL-6 functional vIL-6 OGER vIL P09327 N-Linked glycosylation at the Asn-89 site was required for intracellular production of functional vIL-6, but endoglycosidase-mediated removal of N-linked glycans from secreted vIL-6 did not impair protein function. 21241660 6 28 gly structures 711:720 arg1 PANP PANP structures PUBTATOR PANP 319352 Specific O-glycan structures on PANP were found to be required for PILR recognition of this ligand. 2550193 5 6 gly EPO 610:612 arg1 The two major N-linked oligosaccharides Namalwa EPO The two major N-linked oligosaccharides PUBTATOR Namalwa EPO 2056 The two major N-linked oligosaccharides of Namalwa EPO were fucose-containing tetraantennary and fucose-containing triantennary structures. 20622017 4 62 gly released 711:718 arg2 isolated GGT AND glycans isolated GGT glycans OGER GGT Recent advances in mass spectrometry enabled us to identify the microheterogeneity and relative abundance of glycans on specific glycopeptides and revealed a broader spectrum of glycans than was observed among glycans enzymatically released from isolated GGT. 15152093 5 51 gly glycosylated 869:880 arg1 GCPII GCPII PUBTATOR GCPII 2346 As a type II membrane protein, GCPII is heavily glycosylated. 15152093 5 51 gly glycosylated 869:880 arg1 a type II membrane protein a type II membrane protein PUBTATOR type II membrane protein 10329 As a type II membrane protein, GCPII is heavily glycosylated. 23661698 6 44 gly deglycosylated 911:924 arg1 deglycosylated ephrin-A1 deglycosylated ephrin-A1 PUBTATOR ephrin-A1 1942 Data obtained by surface plasmon resonance confirms that deglycosylated ephrin-A1 does not bind EphA2 with high affinity. 16407218 3 74 gly glycosylated 511:522 arg1 the serum protease inhibitor alpha(2) macroglobulin the serum protease inhibitor alpha(2) macroglobulin PUBTATOR alpha(2) macroglobulin 2 We isolated the serum protease inhibitor alpha(2) macroglobulin (alpha2M), a heavily glycosylated thiol ester protein (TEP) composed of four identical 180-kDa subunits, each of which has eight N-linked glycosylation sites. 23527852 0 86 gly glycoforms 42:51 arg1 new apolipoprotein-CIII glycoforms new apolipoprotein-CIII glycoforms PUBTATOR apolipoprotein-CIII 345 Identification of new apolipoprotein-CIII glycoforms with ultrahigh resolution MALDI-FTICR mass spectrometry of human sera. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Thr146 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Thr126 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser144 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Thr126 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser144 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser144 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 9572875 5 23 part_of IGFBP-6 884:890 arg1 Ser152 IGFBP-6 Thr126, Ser144, Thr145, Thr146, and Ser152 PUBTATOR AminoAcid IGFBP-6 3489 Thr126, Ser144, Thr145, Thr146, and Ser152 Glycosylation sites of IGFBP-6 were identified as Thr126, Ser144, Thr145, Thr146, and Ser152 by using a combination of ESMS and Edman sequencing of tryptic fragments separated by reverse-phase high-pressure liquid chromatography. 25517345 5 9 part_of N241 579:582 arg1 gp120 gp120 N241 PUBTATOR SpecificSite gp120 3700 N241, N262 and N356 However, interestingly, N241, N262 and N356 on gp120 have never been found to be affected after prolonged CBA exposure. 25517345 5 29 part_of N262 585:588 arg1 gp120 gp120 N262 PUBTATOR SpecificSite gp120 3700 N241, N262 and N356 However, interestingly, N241, N262 and N356 on gp120 have never been found to be affected after prolonged CBA exposure. 25517345 5 35 part_of N356 594:597 arg1 gp120 gp120 N356 PUBTATOR SpecificSite gp120 3700 N241, N262 and N356 However, interestingly, N241, N262 and N356 on gp120 have never been found to be affected after prolonged CBA exposure. 1374031 9 105 gly found 1631:1635 arg2 normal urinary hCG AND The major O-linked carbohydrate chains normal urinary hCG The major O-linked carbohydrate chains OGER hCG The major O-linked carbohydrate chains identified are the tetrasaccharide Neu5Ac alpha 2-3Gal beta 1-3(Neu5Ac alpha 2-6)GalNAc-ol and the hexasaccharide Neu5Ac alpha 2-3Gal beta 1-4GlcNAc beta 1-6(Neu5Ac alpha 2-3Gal beta 1-3)GalNAc-ol, both also found in normal urinary hCG. 19088065 10 62 gly modified 1329:1336 arg3 ovarian cancer-derived KLK6 AND alpha2-6-linked sialic acid ovarian cancer-derived KLK6 alpha2-6-linked sialic acid Cterm KLK6 Using a Sambucus nigra agglutinin-monoclonal antibody sandwich enzyme-linked immunosorbent assay approach, it was shown that ovarian cancer-derived KLK6 was modified with alpha2-6-linked sialic acid. 2550193 4 27 gly EPO 528:530 arg1 almost all N-linked oligosaccharide chains Namalwa EPO almost all N-linked oligosaccharide chains PUBTATOR Namalwa EPO 2056 Oligosaccharide structure analyses suggested that almost all N-linked oligosaccharide chains of Namalwa EPO are shared by urinary EPO. 27294781 4 4 gly interface 695:703 arg1 SV2 SV2 interface PUBTATOR SV2 9900 The glycan-binding interface on SV2 is targeted by a human BoNT/A1-neutralizing antibody currently licensed as an antibotulism drug. 16186819 1 14 gly glycoproteins 111:123 arg1 Thrombospondins Thrombospondins PUBTATOR Thrombospondins 7058 Thrombospondins (THBSs) are secreted glycoproteins that have key roles in interactions between cells and the extracellular matrix. 3934016 5 13 gly N-glycosylation 980:994 arg1 human angiotensinogen human angiotensinogen PUBTATOR angiotensinogen 183 For rat angiotensinogen, only 2 of 3 potential sites of N-glycosylation were utilized; in contrast, all 4 potential sites of N-glycosylation of human angiotensinogen were utilized. 1457416 8 85 gly HS-glycoprotein 1656:1670 arg1 alpha 2 HS-glycoprotein alpha 2 HS-glycoprotein PUBTATOR alpha 2 HS-glycoprotein 197 On the basis of these results, the A-chain of alpha 2 HS-glycoprotein was found to possess two biantennary N-glycans and two O-linked trisaccharides. 21606496 5 39 gly 2B4 641:643 arg1 sialylation 2B4 sialylation PUBTATOR 2B4 51744 In contrast, sialylation of 2B4 has a negative impact on ligand binding, as the interaction between 2B4 and CD48 is increased after the removal of sialic acids. 21606496 5 54 gly sialylation 626:636 arg1 2B4 2B4 PUBTATOR 2B4 51744 In contrast, sialylation of 2B4 has a negative impact on ligand binding, as the interaction between 2B4 and CD48 is increased after the removal of sialic acids. 8193552 0 22 gly gonadotropin 131:142 arg1 the disialylated poly-(N-acetyllactosamine)-containing O-linked carbohydrate chains chorionic gonadotropin the disialylated poly-(N-acetyllactosamine)-containing O-linked carbohydrate chains OGER chorionic gonadotropin Structure determination of the disialylated poly-(N-acetyllactosamine)-containing O-linked carbohydrate chains of equine chorionic gonadotropin. 16274239 3 24 gly glycosylated 627:638 arg1 N579Q N579Q PUBTATOR EGFR (N579Q) 13649 To characterize the subpopulation of receptors not glycosylated at N(579), we established a 32D cell line expressing a point mutant of the EGFR (N579Q), which cannot be glycosylated at this position. 12901863 0 26 gly N-glycosylation 29:43 arg1 human ABCC6 human ABCC6 PUBTATOR ABCC6 368 Subcellular localization and N-glycosylation of human ABCC6, expressed in MDCKII cells. 8223648 12 20 gly glycosylated 1359:1370 arg1 glycosylated and nonglycosylated IFN-omega 1 glycosylated and nonglycosylated IFN-omega 1 PUBTATOR IFN-omega 1 3467 Circular dichroism and fluorescence spectroscopy did not reveal any structural differences between glycosylated and nonglycosylated IFN-omega 1. 8223648 12 63 gly nonglycosylated 1376:1390 arg1 glycosylated and nonglycosylated IFN-omega 1 glycosylated and nonglycosylated IFN-omega 1 PUBTATOR IFN-omega 1 3467 Circular dichroism and fluorescence spectroscopy did not reveal any structural differences between glycosylated and nonglycosylated IFN-omega 1. 22967898 3 26 gly N-glycosylation 385:399 arg1 cathepsin V cathepsin V PUBTATOR cathepsin V 1515 In this study, we clarified the role of N-glycosylation of cathepsin V for its functions. 32363391 2 29 gly glycosylated 380:391 arg1 The coronavirus spike (S) protein The coronavirus spike (S) protein OGER S) protein P04004 The coronavirus spike (S) protein, which facilitates viral attachment, entry and membrane fusion is heavily glycosylated and plays a critical role in the elicitation of the host immune response. 8344946 4 80 gly NF-L 830:833 arg1 GlcNAc/mol NF-L GlcNAc/mol PUBTATOR NF-L 4747 In purified neurofilament proteins, the O-GlcNAc modifications occur at a stoichiometry of approximately 0.1 and 0.15 mol of GlcNAc/mol of NF-L and NF-M, respectively. 8344946 4 84 gly NF-M 839:842 arg1 GlcNAc/mol NF-M GlcNAc/mol PUBTATOR NF-M 4741 In purified neurofilament proteins, the O-GlcNAc modifications occur at a stoichiometry of approximately 0.1 and 0.15 mol of GlcNAc/mol of NF-L and NF-M, respectively. 22412906 9 12 gly N-glycosylation 1400:1414 arg1 the GIP receptor the GIP receptor PUBTATOR GIP receptor 2695 N-glycosylation is also required for expression of the GIP receptor at the plasma membrane and efficient GIP potentiation of glucose-induced insulin secretion from the INS-1 pancreatic beta cell line. 24342833 3 35 gly β-catenin 489:497 arg1 the putative O-GlcNAc sites -catenin the putative O-GlcNAc sites PUBTATOR -catenin 1499 OBJECTIVE: The objectives of this investigation were to identify the putative O-GlcNAc sites of β-catenin and the relevance of identified sites in the regulation of β-catenin's localization and transcriptional activity. 21573946 3 3 gly EC-SOD 483:488 arg1 a precise N-glycan profile EC-SOD a precise N-glycan profile PUBTATOR EC-SOD 20657 Thus, a precise N-glycan profile of the recombinant EC-SOD is not available. 29932112 9 12 gly N-glycosylation 1264:1278 arg1 Panx2 Panx2 PUBTATOR Panx2 56666 Our study indicates that N-glycosylation may be important for folding and trafficking of Panx2. 12171601 0 100 gly Glycosylation 0:12 arg1 hPAR2 hPAR2 PUBTATOR hPAR2 2150 Glycosylation of human proteinase-activated receptor-2 (hPAR2): role in cell surface expression and signalling. 12171601 0 100 gly Glycosylation 0:12 arg1 human proteinase-activated receptor-2 human proteinase-activated receptor-2 PUBTATOR proteinase-activated receptor-2 2150 Glycosylation of human proteinase-activated receptor-2 (hPAR2): role in cell surface expression and signalling. 11805077 3 19 gly oligosaccharides 499:514 arg1 the EPO EPO oligosaccharides OGER EPO P01588 Sulfation occurs in a part of the N-linked oligosaccharides in the EPO. 3202829 8 27 gly glycosylation 1074:1086 arg1 human seminal RNase human seminal RNase OGER RNase P07998 The glycosylation pattern of human seminal RNase is very similar to that of the pancreatic enzyme. 17956937 6 70 gly predominant 1207:1217 arg2 the seminal plasma PSA AND high-mannose and hybrid types the seminal plasma PSA high-mannose and hybrid types PUBTATOR PSA 354 In the seminal plasma PSA, high-mannose and hybrid types of oligosaccharides were predominant, and the sialic acids attached to the latter as well as to biantennary oligosaccahrides were exclusively alpha2,6-linked because they were removed by Arthrobacter ureafaciens neuraminidase but resistant to S. pneumoniae neuraminidase. 1657953 3 16 part_of insulin 335:341 arg1 His209 insulin receptor His209 PUBTATOR AminoAcid insulin receptor 3630 His209 His209 (insulin receptor numbering system) is 1 of 2 amino acid residues that are identically conserved in the cysteine-rich domains of insulin receptors, epidermal growth factor receptors, and other homologous receptors. 1657953 3 51 part_of receptor 343:350 arg1 His209 insulin receptor His209 PUBTATOR AminoAcid insulin receptor 3630 His209 His209 (insulin receptor numbering system) is 1 of 2 amino acid residues that are identically conserved in the cysteine-rich domains of insulin receptors, epidermal growth factor receptors, and other homologous receptors. 25081999 5 50 gly hSCP1 1014:1018 arg1 the O-GlcNAc modification site hSCP1 the O-GlcNAc modification site PUBTATOR hSCP1 58190 To gain insight into the PTM of hSCP1, we used the Western blot, immunoprecipitation, succinylayed wheat germ agglutininprecipitation, liquid chromatography-mass spectrometry analyses, and site-directed mutagenesis and identified the Ser41 residue of hSCP1 as the O-GlcNAc modification site. 7781780 1 4 part_of erythropoietin 285:298 arg1 Asn-24 erythropoietin Asn-24 PUBTATOR SpecificSite erythropoietin 2056 Asn-24 A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. 10383441 0 0 gly I 83:83 arg1 mannose phosphorylation DNase I mannose phosphorylation PUBTATOR DNase I 13419 Identification of amino acids that modulate mannose phosphorylation of mouse DNase I, a secretory glycoprotein. 10383441 0 36 gly glycoprotein 98:109 arg1 mouse DNase I mouse DNase I PUBTATOR DNase I 13419 Identification of amino acids that modulate mannose phosphorylation of mouse DNase I, a secretory glycoprotein. 6587378 1 25 gly 2-glycoprotein 128:141 arg1 beta 2-glycoprotein I beta 2-glycoprotein I OGER beta 2-glycoprotein I P02749 We have determined the complete amino acid sequence of beta 2-glycoprotein I (Mr, congruent to 50,000), a human plasma protein that is associated with lipids and binds to platelets but whose function is not yet known. 2243102 2 26 gly glycoproteins 145:157 arg1 lamp-1 lamp-1 PUBTATOR lamp-1 3916 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 2243102 2 26 gly glycoproteins 145:157 arg1 lamp-2 lamp-2 PUBTATOR lamp-2 3920 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 2243102 2 29 gly contain 198:204 arg1 lamp-1 AND 18 and 16 N-glycans lamp-1 18 and 16 N-glycans PUBTATOR lamp-1 3916 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 2243102 2 29 gly contain 198:204 arg1 lamp-2 AND 18 and 16 N-glycans lamp-2 18 and 16 N-glycans PUBTATOR lamp-2 3920 Lysosome membrane glycoproteins, lamp-1 and lamp-2, have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine. 11180632 0 18 gly protein 112:118 arg1 the asparagine-linked sugar chains cerebroside sulfate activator protein the asparagine-linked sugar chains OGER cerebroside sulfate activator protein P17900 Structure of the asparagine-linked sugar chains of porcine kidney and human urine cerebroside sulfate activator protein. 25374123 9 73 gly N-glycosylation 1327:1341 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 As a result, a total of 17 site-specific N-glycopeptides were completely identified in all of the three N-glycosylation sites of vitronectin in human plasma, including 12 N-glycopeptides first reported. 23302862 5 12 gly receptor 1013:1020 arg1 the first leucine-rich-repeat domain insulin receptor the first leucine-rich-repeat domain PUBTATOR insulin receptor P01308 The direct interaction of insulin with the first leucine-rich-repeat domain (L1) of insulin receptor is seen to be sparse, the hormone instead engaging the insulin receptor carboxy-terminal α-chain (αCT) segment, which is itself remodelled on the face of L1 upon insulin binding. 19358553 3 64 gly ribonuclease 645:656 arg1 neutral glycans ribonuclease B neutral glycans Cterm ribonuclease B Glycopeptides derived from glycoproteins containing neutral glycans (ribonuclease B, IgG, and ovalbumin) were initially profiled and yielded excellent and reproducible quantitation (correlation coefficient r = 0.9958, n = 5) when evaluated against a normal phase HPLC 2-AB glycan profile. 17331201 3 48 gly glycoproteins 621:633 arg1 Cbln2 Cbln2 PUBTATOR Cbln2 147381 Here, we demonstrated that in mammalian heterologous cells, Cbln2 and Cbln4 were secreted as N-linked glycoproteins, like Cbln1. 17331201 3 48 gly glycoproteins 621:633 arg1 Cbln4 Cbln4 PUBTATOR Cbln4 140689 Here, we demonstrated that in mammalian heterologous cells, Cbln2 and Cbln4 were secreted as N-linked glycoproteins, like Cbln1. 19478079 0 1 gly modification 29:40 arg1 CCAAT enhancer-binding protein beta CCAAT enhancer-binding protein beta modification PUBTATOR CCAAT enhancer-binding protein beta 1051 O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation. 8636209 7 68 gly glycosylation 864:876 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 Mutagenesis of N-linked glycosylation sites showed that glycosylation of CD3 gamma is not required for TCR assembly and expression. 8636209 7 68 gly glycosylation 864:876 arg1 CD3 gamma CD3 gamma PUBTATOR CD3 gamma 917 Mutagenesis of N-linked glycosylation sites showed that glycosylation of CD3 gamma is not required for TCR assembly and expression. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr81 Thr81 Thr81 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Ser158 Ser158 Ser158 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Ser160 Ser160 Ser160 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr86 Thr86 Thr86 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr156 Thr156 Thr156 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr2 Thr2 Thr2 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 58 gly O-glycosylated 1384:1397 arg1 sIL-15Rα sIL-15Rα OGER sIL Q15468 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 97 gly contained 1331:1339 arg1 IL-15 AND no O-glycans IL-15 no O-glycans PUBTATOR IL-15 3600 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 15616124 3 7 part_of has 416:418 arg1 Human FVII AND N145 Human FVII N145 and N322 Cterm SpecificSite Human FVII 2155 N145 and N322 Human FVII has two N-glycosylation sites (N145 and N322). 23661698 2 23 part_of ephrin-A1 368:376 arg1 the Asn-26 ephrin-A1 the Asn-26 PUBTATOR SpecificSite ephrin-A1 1942 Asn-26 The crystal structure of the ligand-receptor complex revealed a glycosylation on the Asn-26 of ephrin-A1. 10814696 7 18 gly possesses 1476:1484 arg1 human placental arylsulfatase A AND only high-mannose-type oligosaccharides human placental arylsulfatase A only high-mannose-type oligosaccharides PUBTATOR arylsulfatase A 410 The results indicated that human placental arylsulfatase A possesses only high-mannose-type oligosaccharides, of which almost half are core fucosylated. 17563389 2 34 gly glycosylation 324:336 arg1 the NK1R the NK1R PUBTATOR NK1R 6869 We studied the role of N-linked glycosylation in the functioning of the NK1R by constructing three receptor mutants: two single mutants (Asn --> Gln-14 and Asn --> Gln-18) and a double mutant, lacking both glycosylation sites. 10878002 4 24 gly C-Mannosylation 555:569 arg1 human RNase 2 human RNase 2 PUBTATOR RNase 2 6036 C-Mannosylation was first found in human RNase 2 and interleukin-12. 10878002 4 24 gly C-Mannosylation 555:569 arg1 interleukin-12 interleukin-12 OGER interleukin-12 C-Mannosylation was first found in human RNase 2 and interleukin-12. 10878002 4 36 gly found 581:585 arg2 human RNase 2 AND C-Mannosylation human RNase 2 C-Mannosylation PUBTATOR RNase 2 6036 C-Mannosylation was first found in human RNase 2 and interleukin-12. 10878002 4 36 gly found 581:585 arg2 interleukin-12 AND C-Mannosylation interleukin-12 C-Mannosylation OGER interleukin-12 C-Mannosylation was first found in human RNase 2 and interleukin-12. 22226965 7 14 gly PPARγ 1063:1067 arg1 the O-GlcNAc state PPARγ the O-GlcNAc state PUBTATOR PPARγ 19016 Our results suggest that the O-GlcNAc state of PPARγ influences its transcriptional activity and is involved in adipocyte differentiation. 2737288 0 9 gly glycosylation 41:53 arg1 human pancreatic elastase 1 human pancreatic elastase 1 PUBTATOR pancreatic elastase 1 1990 Localization and characterization of the glycosylation site of human pancreatic elastase 1. 27314333 0 47 gly N-Glycosylation 0:14 arg1 Human R-Spondin 1 Human R-Spondin 1 PUBTATOR Human R-Spondin 1 284654 N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability. 19855092 8 64 gly desialylated 1300:1311 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin 11450 In contrast, plasma clearance of desialylated adiponectin was accelerated compared with that of control adiponectin, implicating a role for this modification in determining the half-life of circulating adiponectin. 2209609 0 44 gly glycoprotein 117:128 arg1 a recombinant soluble CD4 glycoprotein a recombinant soluble CD4 glycoprotein PUBTATOR CD4 glycoprotein P01730 The spectrum of N-linked oligosaccharide structures detected by enzymic microsequencing on a recombinant soluble CD4 glycoprotein from Chinese hamster ovary cells. 7514386 1 51 gly glycoprotein 159:170 arg1 CD59 CD59 PUBTATOR CD59 966 CD59 is an 18-kDa glycoprotein widely expressed on human cells. 2059624 2 75 gly oligosaccharides 441:456 arg1 Asn289 Asn289 Asn289 AminoAcid Asn289 Previous studies from this laboratory have established that lepidopteran insect cells possess the glycosylation machinery needed to assemble N-linked complex-type oligosaccharides on Asn289 of recombinant human plasminogen (r-HPg). 9705299 1 3 gly glycoprotein 196:207 arg1 glucose-6-phosphatase glucose-6-phosphatase OGER glucose-6-phosphatase P35575 Deficiency of glucose-6-phosphatase (G6Pase), an endoplasmic reticulum transmembrane glycoprotein, causes glycogen storage disease type 1a. 29095159 1 26 gly glycoprotein 284:295 arg1 myelin-associated glycoprotein myelin-associated glycoprotein OGER myelin-associated glycoprotein P20916 The Nogo Receptor (NgR) is a glycophosphatidylinositol-anchored cell-surface protein and is a receptor for three myelin-associated inhibitors of regeneration: myelin-associated glycoprotein, Nogo66 and oligodendrocyte myelin glycoprotein. 29095159 1 57 gly glycoprotein 332:343 arg1 oligodendrocyte myelin glycoprotein oligodendrocyte myelin glycoprotein PUBTATOR oligodendrocyte myelin glycoprotein 4974 The Nogo Receptor (NgR) is a glycophosphatidylinositol-anchored cell-surface protein and is a receptor for three myelin-associated inhibitors of regeneration: myelin-associated glycoprotein, Nogo66 and oligodendrocyte myelin glycoprotein. 17711303 5 65 gly contains 876:883 arg1 the hKOR AND O-linked glycan the hKOR O-linked glycan PUBTATOR hKOR 4986 FLAG-hKOR was reduced to lower Mr bands by neuraminidase and O-glycosidase, indicating that the hKOR contains O-linked glycan. 8329384 0 26 part_of IX 165:166 arg1 serine 61 factor IX serine 61 OGER SpecificSite factor IX P00740 serine 61 Identification and structural analysis of the tetrasaccharide NeuAc alpha(2-->6)Gal beta(1-->4)GlcNAc beta(1-->3)Fuc alpha 1-->O-linked to serine 61 of human factor IX. 19671700 5 64 gly containing 908:917 arg1 TSR1 AND a Glc-Fuc disaccharide TSR1 a Glc-Fuc disaccharide PUBTATOR TSR1 55720 Analysis of tryptic fragments of recombinant human punctin-1 by mass spectrometry identified a peptide derived from TSR1 containing the (36)WDAWGPWSECSRTC(49) sequence of interest modified with two mannose residues and a Glc-Fuc disaccharide (O-fucosylation). 19671700 5 64 gly containing 908:917 arg1 TSR1 AND O-fucosylation TSR1 O-fucosylation PUBTATOR TSR1 55720 Analysis of tryptic fragments of recombinant human punctin-1 by mass spectrometry identified a peptide derived from TSR1 containing the (36)WDAWGPWSECSRTC(49) sequence of interest modified with two mannose residues and a Glc-Fuc disaccharide (O-fucosylation). 7511099 2 81 gly disialo-AFP 788:798 arg1 alpha 2-->3 sialylated galactose AFP alpha 2-->3 sialylated galactose PUBTATOR AFP 174 By means of neuraminidase and/or beta-galactosidase treatment, AFP-P2 was identified as alpha 2-->6 disialo-AFP, AFP-P3 as having biantennary structures with alpha 2-->6 monosialylated galactose of the Mannose (Man) alpha 1-->6 arm, AFP-P4 as having alpha 2-->6 monosialylated galactose of the Man alpha 1-->3 arm, and AFP-P5 as disialo-AFP with alpha 2-->3 sialylated galactose of the Man alpha 1-->6 antenna with the alpha 2-->6 sialylated galactose of the other antenna. 7511099 2 66 gly monosialylated 629:642 arg1 AFP-P2 AFP PUBTATOR AFP 174 By means of neuraminidase and/or beta-galactosidase treatment, AFP-P2 was identified as alpha 2-->6 disialo-AFP, AFP-P3 as having biantennary structures with alpha 2-->6 monosialylated galactose of the Mannose (Man) alpha 1-->6 arm, AFP-P4 as having alpha 2-->6 monosialylated galactose of the Man alpha 1-->3 arm, and AFP-P5 as disialo-AFP with alpha 2-->3 sialylated galactose of the Man alpha 1-->6 antenna with the alpha 2-->6 sialylated galactose of the other antenna. 28973932 5 12 gly glycosylation 1190:1202 arg1 hepatocyte growth factor receptor hepatocyte growth factor receptor PUBTATOR hepatocyte growth factor receptor 4233 In addition, O-Man glycosylation of IPT/TIG domains of plexins and hepatocyte growth factor receptor was not affected in TMTC KO cells, suggesting the existence of yet another O-Man glycosylation machinery. 25458834 1 35 gly O-glycoprotein 149:162 arg1 Podoplanin Podoplanin OGER Podoplanin Q86YL7 Podoplanin is a transmembrane O-glycoprotein that binds to C-type lectin-like receptor 2 (CLEC-2). 19343721 2 17 gly hPC 505:507 arg1 complex di- and tri-sialylated structures hPC complex di- and tri-sialylated structures OGER hPC P11498 The N-glycans of hPC are complex di- and tri-sialylated structures, and we measured 78% site occupancy at Asn-329 (the Asn-X-Cys sequon). 19343721 2 17 gly hPC 505:507 arg1 The N-glycans hPC The N-glycans OGER hPC P11498 The N-glycans of hPC are complex di- and tri-sialylated structures, and we measured 78% site occupancy at Asn-329 (the Asn-X-Cys sequon). 23001782 4 28 gly glycans 814:820 arg1 intact hSHBG hSHBG glycans PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 91 gly hSHBG 832:836 arg1 detailed glycan structures hSHBG detailed glycan structures PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 91 gly hSHBG 832:836 arg1 glycans hSHBG glycans PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 91 gly hSHBG 832:836 arg1 monosaccharide compositions hSHBG monosaccharide compositions PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 117 gly structures 766:775 arg1 intact hSHBG hSHBG structures PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 124 gly compositions 736:747 arg1 intact hSHBG hSHBG compositions PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 18340083 5 15 gly released 619:626 arg1 mouse GPIHBP1 AND The N-linked glycan mouse GPIHBP1 The N-linked glycan PUBTATOR GPIHBP1 68453 The N-linked glycan could be released from mouse GPIHBP1 with N-glycosidase F, endoglycosidase H, or endoglycosidase F1. 18061361 8 7 gly N-glycosylated 1452:1465 arg1 nearly all NCBE nearly all NCBE PUBTATOR NCBE 94229 By Western blot, nearly all NCBE in mouse and rat brain is highly N-glycosylated (approximately 150 kDa). 10712595 7 59 gly glycosylation 1329:1341 arg1 human antithrombin human antithrombin PUBTATOR antithrombin 462 Furthermore, the invariant third-position Ser137 at this glycosylation site of mammalian and chicken antithrombins is substituted by Thr in the salmon, a replacement that has been shown to induce full glycosylation in human antithrombin. 1569071 9 16 gly unglycosylated 1800:1813 arg1 precursor IGF-II precursor IGF-II PUBTATOR IGF-II P01344 This was the Mr value that would be predicted for an unglycosylated form of precursor IGF-II that had a carboxyl-terminal end at or near Lys88. 10704524 10 88 gly A 1763:1763 arg1 the oligosaccharide structures alpha-galactosidase A the oligosaccharide structures OGER alpha-galactosidase A P06280 Comparison of the oligosaccharide structures of alpha-GalNAc and alpha-galactosidase A, an evolutionary-related and highly homologous exoglycosidase, indicated that alpha-GalNAc had more completed complex chains, presumably due to differences in enzyme structure/domains, rate of biosynthesis, and/or aggregation of the overexpressed recombinant enzymes. 21712440 2 49 gly glycoprotein 411:422 arg1 APP APP OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 21712440 2 6 gly carrying 424:431 arg1 APP AND O-glycans APP O-glycans OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 21712440 2 6 gly carrying 424:431 arg1 APP AND N- APP N- OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 18467335 0 14 gly inhibitor 42:50 arg1 N-glycans protein C inhibitor N-glycans OGER protein C inhibitor P05154 N-glycans and the N terminus of protein C inhibitor affect the cofactor-enhanced rates of thrombin inhibition. 19690161 0 0 gly glycans 17:23 arg1 Asn-89 Asn-89 Asn-89 SpecificSite Asn-89 Complex N-linked glycans on Asn-89 of Kaposi sarcoma herpes virus-encoded interleukin-6 mediate optimal function by affecting cytokine protein conformation. 23242014 8 23 gly glycosylation 1074:1086 arg1 the HCV envelope protein E2 the HCV envelope protein E2 Cterm E2 Here, we have investigated in detail the O-linked glycosylation of the HCV envelope protein E2 expressed in and isolated from human embryonic kidney (HEK 293) cells. 11706042 1 24 gly multi-protein 141:153 arg1 The lipopolysaccharide (LPS) receptor multi The lipopolysaccharide (LPS) receptor OGER multi The lipopolysaccharide (LPS) receptor is a multi-protein complex that consists of at least three proteins, CD14, TLR4, and MD-2. 3264725 9 2 gly VIIa 1858:1861 arg1 the overall carbohydrate compositions factor VIIa the overall carbohydrate compositions Cterm factor VIIa Besides minor differences in the sialic acid and fucose contents, the overall carbohydrate compositions were nearly identical in recombinant factor VIIa and human plasma factor VIIa. 3264725 9 75 gly VIIa 1829:1832 arg1 the overall carbohydrate compositions factor VIIa the overall carbohydrate compositions Cterm factor VIIa Besides minor differences in the sialic acid and fucose contents, the overall carbohydrate compositions were nearly identical in recombinant factor VIIa and human plasma factor VIIa. 19038966 3 66 gly glycosylated 449:460 arg1 the fully glycosylated TPP1 precursor the fully glycosylated TPP1 precursor PUBTATOR TPP1 precursor 1200 We crystallized the fully glycosylated TPP1 precursor under conditions that implied partial autocatalytic cleavage between the prosegment and the catalytic domain. 16201406 1 18 gly glycoprotein 203:214 arg1 Human lactoferrin Human lactoferrin OGER Human lactoferrin P02788 Human lactoferrin (hLF) is an iron-binding glycoprotein involved in the host defence against infection and excessive inflammation. 18340083 4 33 gly glycosylated 576:587 arg1 Human GPIHBP1 Human GPIHBP1 PUBTATOR Human GPIHBP1 338328 Human GPIHBP1 is also glycosylated. 8702840 7 36 part_of NF-M 1099:1102 arg1 Ser48 NF-M Ser48 PUBTATOR AminoAcid NF-M 4741 Ser34 and Ser48 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 47 part_of NF-L 1127:1130 arg1 Ser48 NF-L Ser48 PUBTATOR AminoAcid NF-L 4747 Ser34 and Ser48 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 36 part_of NF-M 1099:1102 arg1 Thr19 NF-M Thr19 and Ser34 PUBTATOR AminoAcid NF-M 4741 Thr19 and Ser34 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 47 part_of NF-L 1127:1130 arg1 Thr19 NF-L Thr19 and Ser34 PUBTATOR AminoAcid NF-L 4747 Thr19 and Ser34 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 17956937 8 37 gly attached 1824:1831 arg2 PSA AND oligosaccharides PSA oligosaccharides PUBTATOR PSA 354 The results suggest that identification of alpha2,3-linked sialic acids on PSA potentially discriminates malignant from benign conditions, if the analysis is applied to oligosaccharides specifically attached to the N-glycosylation site of PSA in either a free or a complexed form in the serum. 17956937 8 104 gly N-glycosylation 1840:1854 arg1 PSA PSA PUBTATOR PSA 354 The results suggest that identification of alpha2,3-linked sialic acids on PSA potentially discriminates malignant from benign conditions, if the analysis is applied to oligosaccharides specifically attached to the N-glycosylation site of PSA in either a free or a complexed form in the serum. 3934016 4 16 gly N-Glycosylation 778:792 arg1 secreted angiotensinogen secreted angiotensinogen PUBTATOR angiotensinogen 183 N-Glycosylation of secreted angiotensinogen was inhibited using tunicamycin. 24509848 5 54 part_of residue 680:686 arg1 N-glycosylation t N-glycosylation residue PUBTATOR SpecificSite t N-glycosylation 780 Asn(211) residue Here, we report that N-glycosylation at the Asn(211) residue plays a unique role in the control of DDR1 dimerization and autophosphorylation. 16698036 1 70 gly Lysosomal 137:145 arg1 Hex A Lysosomal beta-hexosaminidase A Hex A PUBTATOR Lysosomal beta-hexosaminidase A 3073 Lysosomal beta-hexosaminidase A (Hex A) is essential for the degradation of GM2 gangliosides in the central and peripheral nervous system. 16698036 1 75 gly beta-hexosaminidase 147:165 arg1 Hex A Lysosomal beta-hexosaminidase A Hex A PUBTATOR Lysosomal beta-hexosaminidase A 3073 Lysosomal beta-hexosaminidase A (Hex A) is essential for the degradation of GM2 gangliosides in the central and peripheral nervous system. 8687384 0 45 gly glycosylation 4:16 arg1 Bowes melanoma tissue plasminogen activator Bowes melanoma tissue plasminogen activator PUBTATOR tissue plasminogen activator P00750 The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans. 11676606 8 46 part_of CD154 1220:1224 arg1 asparagine 240 CD154 asparagine 240 PUBTATOR SpecificSite CD154 959 asparagine 240 Together, these results indicate that the presence of varied types of N-linked glycans on asparagine 240 of CD154 does not play a significant role in the CD40-CD154 interactions. 2110822 4 114 gly SAP-1 878:882 arg1 Sugar chains SAP-1 Sugar chains PUBTATOR SAP-1 5660 Sugar chains of SAP-1 purified from normal human liver and from GM1 gangliosidosis (type 1) liver were different from each other, although both of them were derived from complex-type sugar chains. 9933650 0 81 gly interleukin-1 52:64 arg1 novel carbohydrate binding activity interleukin-1 novel carbohydrate binding activity PUBTATOR interleukin-1 3552 Detection of novel carbohydrate binding activity of interleukin-1. 24692546 3 6 gly glycosylated 516:527 arg1 fully glycosylated FSH fully glycosylated FSH OGER FSH The model predicts that FSHR binds Asnα(52)-deglycosylated FSH at a 3-fold higher capacity than fully glycosylated FSH. 24692546 3 14 gly -deglycosylated 457:471 arg1 Asnα(52)-deglycosylated FSH Asnα(52)-deglycosylated FSH OGER FSH The model predicts that FSHR binds Asnα(52)-deglycosylated FSH at a 3-fold higher capacity than fully glycosylated FSH. 1445902 4 42 gly deglycosylated 717:730 arg1 glycosylated and deglycosylated IL-3 glycosylated and deglycosylated IL-3 PUBTATOR IL-3 16187 Liquid secondary ion mass spectrometric analysis was carried out on the reduced tryptic and endopeptidase lysyl-C peptides of glycosylated and deglycosylated IL-3. 1445902 4 66 gly glycosylated 700:711 arg1 glycosylated and deglycosylated IL-3 glycosylated and deglycosylated IL-3 PUBTATOR IL-3 16187 Liquid secondary ion mass spectrometric analysis was carried out on the reduced tryptic and endopeptidase lysyl-C peptides of glycosylated and deglycosylated IL-3. 16474139 4 87 gly glycosylated 836:847 arg1 The M protein The M protein OGER M protein P54296 The M protein plays a crucial role in coronavirus assembly and is glycosylated in all coronaviruses, either by N-linked or by O-linked oligosaccharides. 20356926 4 36 gly glycosylation 392:404 arg1 CREB-H CREB-H PUBTATOR CREB-H 84699 In this study we characterized N-linked glycosylation of CREB-H in the luminal domain at the C-terminus. 16679516 0 25 part_of thyroglobulin 73:85 arg1 Ser-2730 thyroglobulin Ser-2730 OGER SpecificSite thyroglobulin P01266 Ser-2730 A single chondroitin 6-sulfate oligosaccharide unit at Ser-2730 of human thyroglobulin enhances hormone formation and limits proteolytic accessibility at the carboxyl terminus. 2498325 2 37 gly glycosylation 96:108 arg1 human apolipoprotein (apo) E human apolipoprotein (apo) E PUBTATOR apolipoprotein (apo) E 348 The glycosylation of human apolipoprotein (apo) E was examined with purified plasma apoE and apoE produced by transfected cell lines. 9228058 7 36 part_of FasL 1238:1241 arg1 Tyr-218 FasL Tyr-218 PUBTATOR SpecificSite FasL 356 Tyr-218 Although the cytotoxic activity of mutant Y218D was unaltered, mutant Y218R was inactive, correlating with the prediction that Tyr-218 of FasL interacts with a cluster of three basic amino acid side chains of Fas. 17117926 9 29 gly glycosylation 1189:1201 arg1 Wnt-5a Wnt-5a PUBTATOR Wnt-5a 7474 In contrast, glycosylation was necessary for the secretion of Wnt-5a, but not essential for the actions of Wnt-5a. 16186819 2 5 gly glycosylated 269:280 arg1 human THBS2 human THBS2 PUBTATOR THBS2 7058 Here, we describe the 2.6-A-resolution crystal structure of the glycosylated signature domain of human THBS2, which includes three epidermal growth factor-like modules, 13 aspartate-rich repeats and a lectin-like module. 26467158 8 76 gly modification 1418:1429 arg1 BACE1 BACE1 modification PUBTATOR BACE1 23821 Point mutations at two N-glycosylation sites (Asn(153) and Asn(223)) abolish the bisecting GlcNAc modification on BACE1. 19951703 6 9 gly unglycosylated 826:839 arg1 unglycosylated UGT1A9 unglycosylated UGT1A9 PUBTATOR UGT1A9 54600 To evaluate the role of glycosylation in the enzyme activity, we produced unglycosylated UGT1A9 by treating HEK293 cells transiently transfected with expression plasmid with tunicamycin. 16201406 4 2 gly found 819:823 arg2 natural hLF AND complex-type glycans natural hLF complex-type glycans PUBTATOR hLF 3131 Even though rhLF contains oligomannose- and hybrid-type N-linked glycans next to complex-type glycans, which are the only glycans found on natural hLF, the structures are identical within the experimental error (r.m.s. deviation of only 0.28 A for the main-chain atoms). 16201406 4 2 gly found 819:823 arg2 natural hLF AND the only glycans natural hLF the only glycans PUBTATOR hLF 3131 Even though rhLF contains oligomannose- and hybrid-type N-linked glycans next to complex-type glycans, which are the only glycans found on natural hLF, the structures are identical within the experimental error (r.m.s. deviation of only 0.28 A for the main-chain atoms). 16201406 4 19 gly contains 706:713 arg1 rhLF AND oligomannose- and hybrid-type N-linked glycans rhLF oligomannose- and hybrid-type N-linked glycans OGER rhLF P02788 Even though rhLF contains oligomannose- and hybrid-type N-linked glycans next to complex-type glycans, which are the only glycans found on natural hLF, the structures are identical within the experimental error (r.m.s. deviation of only 0.28 A for the main-chain atoms). 1997323 14 70 gly contained 1693:1701 arg1 rTf AND several tetrasialylated diantennary glycans rTf several tetrasialylated diantennary glycans Cterm rTf 24825 A further preparation, obtained from the most anionic DEAE-cellulose fraction (peak V) or rTf contained several tetrasialylated diantennary glycans whose precise structures remain to be established in future studies. 27966990 0 44 gly N-Glycosylation 14:28 arg1 Endothelial Cell Receptor Tyrosine Kinase VEGFR-2 Endothelial Cell Receptor Tyrosine Kinase VEGFR-2 OGER VEGFR-2 P35968 Site-Specific N-Glycosylation of Endothelial Cell Receptor Tyrosine Kinase VEGFR-2. 6118137 13 35 gly glycoprotein 1324:1335 arg1 the Thy-1 glycoprotein the Thy-1 glycoprotein PUBTATOR Thy-1 glycoprotein 24832 The sequence of the Thy-1 glycoprotein showed homologies with immunoglobulin domains. 12063277 5 10 gly glycosylation 746:758 arg1 functional HERG channels functional HERG channels PUBTATOR HERG channels 3757 Our results show that N598 is the only site for N-linked glycosylation and that glycosylation is not required for the cell surface expression of functional HERG channels. 10092871 3 71 gly unglycosylated 456:469 arg1 MMP-1 MMP-1 PUBTATOR MMP-1 4312 MMP-1 is secreted as both glycosylated and unglycosylated species, and the two forms have been shown to be identical with respect to substrate specificity, specific activity and inhibitory profile. 10092871 3 82 gly glycosylated 439:450 arg1 MMP-1 MMP-1 PUBTATOR MMP-1 4312 MMP-1 is secreted as both glycosylated and unglycosylated species, and the two forms have been shown to be identical with respect to substrate specificity, specific activity and inhibitory profile. 14693913 5 2 gly contain 868:874 arg1 hBSSL AND 16 Pro-rich 11-amino-acid repeats hBSSL The C-terminal 192 residues 16 Pro-rich 11-amino-acid repeats PUBTATOR hBSSL 1056 residues The C-terminal 192 residues of hBSSL contain 16 Pro-rich 11-amino-acid repeats, which include 32 Ser/Thr residues as potential O-glycosylation sites. 18930737 1 26 gly alpha2-glycoprotein 218:236 arg1 zinc alpha2-glycoprotein zinc alpha2-glycoprotein PUBTATOR zinc alpha2-glycoprotein 563 This is the first report on the formation of a complex between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP). 18930737 1 26 gly alpha2-glycoprotein 218:236 arg1 ZAG ZAG PUBTATOR ZAG 563 This is the first report on the formation of a complex between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP). 12171601 1 4 gly glycosylation 150:162 arg1 human proteinase-activated receptor-2 human proteinase-activated receptor-2 PUBTATOR proteinase-activated receptor-2 2150 We have analysed the role of N-linked glycosylation in regulating human proteinase-activated receptor-2 (hPAR(2)) expression and function. 12171601 1 4 gly glycosylation 150:162 arg1 hPAR(2) hPAR(2) PUBTATOR hPAR(2) 2150 We have analysed the role of N-linked glycosylation in regulating human proteinase-activated receptor-2 (hPAR(2)) expression and function. 19413349 3 43 gly glycosylated 421:432 arg1 melanopsin melanopsin PUBTATOR melanopsin 192223 To investigate if melanopsin is N-linked glycosylated and whether N-bound glycans influence the response of melanopsin to light as evidenced by Fos mRNA induction, we transfected PC12 cells to stably express rat wild-type melanopsin or mutant melanopsin lacking both N-linked glycosylation sites. 22547800 4 20 gly channel 791:797 arg1 a leucine-rich repeat (LRR)-containing membrane protein BK channel a leucine-rich repeat (LRR)-containing membrane protein OGER BK channel We recently identified a leucine-rich repeat (LRR)-containing membrane protein, LRRC26, as a BK channel auxiliary subunit, which causes an unprecedented large negative shift (∼140 mV) in voltage dependence of channel activation. 22547800 4 23 gly BK 788:789 arg1 a leucine-rich repeat (LRR)-containing membrane protein BK channel a leucine-rich repeat (LRR)-containing membrane protein OGER BK channel We recently identified a leucine-rich repeat (LRR)-containing membrane protein, LRRC26, as a BK channel auxiliary subunit, which causes an unprecedented large negative shift (∼140 mV) in voltage dependence of channel activation. 10712595 9 2 gly have 1629:1632 arg1 Salmon antithrombin AND three complex oligosaccharide side chains Salmon antithrombin three complex oligosaccharide side chains PUBTATOR antithrombin 462 Salmon antithrombin appears to have three complex oligosaccharide side chains containing sialic acid terminally linked alpha(2-3) to galactose, while trace amounts of Galbeta(1-4)GlcNAc suggest microheterogeneity due to partial loss of sialic acid. 18642129 6 30 gly glycoprotein 912:923 arg1 FVII FVII OGER FVII P08709 A MALDI-MS analysis of the native protein indicated that FVII is a 50.1 kDa glycoprotein modified on two sites by diantennary, disialylated non-fucosylated (A2S2) glycans. 2226797 0 48 gly variant 64:70 arg1 Carbohydrate structures tissue plasminogen activator variant Carbohydrate structures PUBTATOR tissue plasminogen activator variant P00750 Carbohydrate structures of a human tissue plasminogen activator variant expressed in recombinant Chinese hamster ovary cells. 6689265 0 5 gly protein 84:90 arg1 the O-glycan chain vitamin-D binding protein the O-glycan chain PUBTATOR vitamin-D binding protein 2638 Isolation and characterization of the O-glycan chain of the human vitamin-D binding protein. 27384988 7 41 gly glycosylation 672:684 arg1 rpS3 rpS3 PUBTATOR rpS3 6188 N-linked glycosylation of rpS3 was confirmed as necessary for rpS3 secretion into culture media via the ER-Golgi dependent pathway. 21148085 0 52 gly factor-D 55:62 arg1 Structural determinants vascular endothelial growth factor-D Structural determinants PUBTATOR vascular endothelial growth factor-D 2277 Structural determinants of vascular endothelial growth factor-D receptor binding and specificity. 11371615 4 2 gly modified 644:651 arg3 RNA polymerase II AND O-GlcNAc RNA polymerase II O-GlcNAc OGER RNA polymerase II Meanwhile, transcription factors and RNA polymerase II can be modified by O-GlcNAc. 22826440 8 45 gly Zinc 1653:1656 arg1 the first human O-GlcNAc-6-phosphate modified protein Zinc finger protein 462 the first human O-GlcNAc-6-phosphate modified protein PUBTATOR Zinc finger protein 462 58499 By re-analyzing mass spectrometric data from human embryonic and induced pluripotent stem cells, our study also identified Zinc finger protein 462 (ZNF462) as the first human O-GlcNAc-6-phosphate modified protein. 22826440 8 85 gly finger 1658:1663 arg1 the first human O-GlcNAc-6-phosphate modified protein Zinc finger protein 462 the first human O-GlcNAc-6-phosphate modified protein PUBTATOR Zinc finger protein 462 58499 By re-analyzing mass spectrometric data from human embryonic and induced pluripotent stem cells, our study also identified Zinc finger protein 462 (ZNF462) as the first human O-GlcNAc-6-phosphate modified protein. 9933650 11 2 gly has 1580:1582 arg1 thyroid-stimulating hormone AND the N-acetylgalactosamine residue thyroid-stimulating hormone the N-acetylgalactosamine residue OGER thyroid-stimulating hormone Since IL-1beta did not bind to thyroid-stimulating hormone, which has the sulfate group at C-4 of the N-acetylgalactosamine residue in its N-linked sugar chains, the binding of IL-1beta toward oligosaccharides in fraction AR was considered to be highly specific. 18952059 6 29 gly glycosylation 755:767 arg1 IZUMO IZUMO PUBTATOR IZUMO 73456 These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis. 16014566 1 11 gly glycoprotein 109:120 arg1 human glycoprotein VI (GPVI) human glycoprotein VI (GPVI) PUBTATOR glycoprotein VI 51206 Using recombinant human glycoprotein VI (GPVI), we evaluated the effect of N-linked glycosylation at the consensus site Asparagine92-Glycine-Serine94 (N92GS94) on binding of this platelet-specific receptor to its ligands, human type I collagen, collagen-related peptide (CRP), and the snake venom C-type lectin convulxin (CVX). 10988252 3 72 gly glycoprotein 433:444 arg1 sEGFR sEGFR PUBTATOR EGFR 1956 The human epidermoid carcinoma A431 cell line secretes a soluble 105 kDa glycoprotein (sEGFR) that represents the extracellular domain of the membrane-bound form, and its glycosylation pattern has been investigated. 17222411 0 29 gly glycosylation 11:23 arg1 human podoplanin human podoplanin OGER podoplanin Q86YL7 Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor. 16274239 0 63 part_of receptor 78:85 arg1 Asn-579 epidermal growth factor receptor Asn-579 PUBTATOR SpecificSite epidermal growth factor receptor 13649 Asn-579 Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor. 12901863 3 10 gly glycosylated 455:466 arg1 The human ABCC6 The human ABCC6 PUBTATOR ABCC6 368 The human ABCC6 in MDCKII cells was found to be glycosylated, in contrast to the underglycosylated form of the protein, as expressed in Sf9 cells. 18585350 3 38 gly glycoprotein 484:495 arg1 CALHM1 CALHM1 PUBTATOR CALHM1 255022 We show that CALHM1 encodes a multipass transmembrane glycoprotein that controls cytosolic Ca(2+) concentrations and Abeta levels. 16622833 2 52 gly N-glycosylation 245:259 arg1 A1PI A1PI PUBTATOR A1PI 5265 The three N-glycosylation sites of A1PI contain diantennary N-glycans but also triantennary and even traces of tetraantennary structures leading to the typical IEF pattern observed for A1PI. 16622833 2 16 gly contain 275:281 arg1 A1PI AND diantennary N-glycans but also triantennary and even traces A1PI diantennary N-glycans but also triantennary and even traces PUBTATOR A1PI 5265 The three N-glycosylation sites of A1PI contain diantennary N-glycans but also triantennary and even traces of tetraantennary structures leading to the typical IEF pattern observed for A1PI. 1371281 5 31 gly contain 678:684 arg1 cytokeratin 8 and 18 AND single O-linked N-acetylglucosamine residues cytokeratin 8 and 18 single O-linked N-acetylglucosamine residues PUBTATOR cytokeratin 8 3856 beta-Elimination of the [3H]galactose- labeled CK8/18 generated the disaccharide N-acetyllactosaminitol, indicating that cytokeratin 8 and 18 contain single O-linked N-acetylglucosamine residues. 1544894 5 18 gly fucosylated 943:953 arg1 factor XII factor XII OGER factor XII P00748 We found that factor XII is fully fucosylated at Thr-90. 30659065 11 16 part_of IgM 1842:1844 arg1 N272 IgM sites N46, N209, and N272 OGER SpecificSite IgM P01871 sites N46, N209, and N272 IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms. 30659065 11 16 part_of IgM 1842:1844 arg1 N46 IgM sites N46, N209, and N272 OGER SpecificSite IgM P01871 sites N46, N209, and N272 IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms. 30659065 11 16 part_of IgM 1842:1844 arg1 IgM sites IgM sites N46, N209, and N272 OGER SpecificSite IgM P01871 sites N46, N209, and N272 IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms. 30659065 11 16 part_of IgM 1842:1844 arg1 N46 IgM sites N46, N209, and N272 OGER SpecificSite IgM P01871 sites N46, N209, and N272 IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms. 30659065 11 16 part_of IgM 1842:1844 arg1 IgM sites IgM sites N46, N209, and N272 OGER SpecificSite IgM P01871 sites N46, N209, and N272 IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms. 30659065 11 16 part_of IgM 1842:1844 arg1 IgM sites IgM sites N46, N209, and N272 OGER SpecificSite IgM P01871 sites N46, N209, and N272 IgM sites N46, N209, and N272 displayed mostly complex glycans, whereas sites N279 and N439 displayed higher relative abundances of high-mannose glycoforms. 9233787 1 9 gly glycoprotein 166:177 arg1 BM-40 BM-40 OGER BM-40 P09486 BM-40 (also known as SPARC or osteonectin) is an anti-adhesive secreted glycoprotein involved in tissue remodelling. 20729549 4 65 gly K8/18 643:647 arg1 O-GlcNAcylation K8/18 O-GlcNAcylation PUBTATOR K8/18 3856 Here, by comparing immortalized (Chang) and transformed hepatocyte (HepG2) cell lines, we have demonstrated that O-GlcNAcylation of K8/18 exhibits a positive correlation with their solubility (Nonidet P-40 extractability). 12768205 4 11 gly has 556:558 arg1 Fcalpha AND interdomain N-linked carbohydrates Fcalpha interdomain N-linked carbohydrates Cterm Fcalpha P01876 Fcalpha resembles the Fcs of immunoglobulins IgG and IgE, but has differently located interchain disulphide bonds and external rather than interdomain N-linked carbohydrates. 2110822 1 89 gly protein 215:221 arg1 Asparagine-linked sugar chains activator protein 1 Asparagine-linked sugar chains PUBTATOR activator protein 1 5660 Asparagine-linked sugar chains of sphingolipid activator protein 1 (SAP-1) purified from normal human liver and GM1 gangliosidosis (type 1) liver were comparatively investigated. 24334224 4 49 part_of found 917:921 arg2 endogenous human G-CSF AND Thr134 endogenous human G-CSF Thr134 PUBTATOR AminoAcid G-CSF 1440 Thr134 Our results demonstrated that Thr134, the equivalent O-linked glycosylation site found on endogenous human G-CSF, is the only site modified with a single mannose, allowing glycoengineered P. pastoris to be used as a viable production platform for therapeutic rhG-CSF. 8670172 14 8 gly CD59 1926:1929 arg1 N-linked oligosaccharide CD59 N-linked oligosaccharide PUBTATOR CD59 966 These results document the structural heterogeneity of both the GPI anchor and N-linked oligosaccharide of CD59 and demonstrate that the phospholipid tail is needed for the full functional activity of CD59. 24365146 6 4 gly unglycosylated 909:922 arg1 unglycosylated Nox1 unglycosylated Nox1 PUBTATOR Nox1 27035 Superoxide production by unglycosylated Nox1 is largely dependent on p22(phox), which is abrogated by glutamine substitution for Pro-156 in p22(phox), a mutation leading to a defective interaction with the Nox1-activating protein Noxo1. 23431362 10 69 part_of gp120 1875:1879 arg1 the N332-region HIV-1 gp120 the N332-region PUBTATOR SpecificSite HIV-1 gp120 155971 N332 Overall, our results add to the growing body of evidence that the human immune system is capable of recognizing the N332-region of HIV-1 gp120 in diverse ways. 16332679 10 28 gly hyperglycosylation 1617:1634 arg1 Sp1 Sp1 OGER Sp1 P08047 Treatment of cells with streptozotocin (a potent inhibitor of O-GlcNAcase) led to hyperglycosylation of Sp1 that failed to be significantly phosphorylated. 7525874 12 76 gly glycosylated 1650:1661 arg1 this protein this protein OGER protein can P35658 According to the structural features (a high degree of fucosylation, high amounts of bisecting N-acetylglucosamine, as well as terminal N-acetylglucosamine and galactose residues, and significant amounts of N-acetylneuraminic acid in alpha 2,3 linkage), this protein can be classified as "brain-type" glycosylated. 8286855 1 46 gly sialoglycoprotein 144:160 arg1 glycophorin A glycophorin A PUBTATOR glycophorin A 2993 The human red blood cell sialoglycoprotein, glycophorin A (GpA), contains a 'mucin-like' extensively O-glycosylated extracellular domain which carries the MN blood group antigens. 1997323 0 31 gly serotransferrin 39:53 arg1 Carbohydrate microheterogeneity serotransferrin Carbohydrate microheterogeneity PUBTATOR serotransferrin 24825 Carbohydrate microheterogeneity of rat serotransferrin. 1997323 0 33 gly microheterogeneity 13:30 arg1 rat serotransferrin rat serotransferrin PUBTATOR serotransferrin 24825 Carbohydrate microheterogeneity of rat serotransferrin. 10889209 6 0 gly glycosylated 1021:1032 arg1 GIRK4 GIRK4 PUBTATOR GIRK4 3762 This finding may partly account for the reason that GIRK4 is not glycosylated at Asn(132), either as a homomer or when coexpressed with GIRK1. 8435067 6 32 gly alpha-glucosidase 862:878 arg1 the oligosaccharide side chains lysosomal alpha-glucosidase the oligosaccharide side chains PUBTATOR lysosomal alpha-glucosidase 2548 Evidence is presented that at least two of the oligosaccharide side chains of human lysosomal alpha-glucosidase are phosphorylated. 2243102 6 15 part_of Asn-34 953:958 arg1 lamp-1 lamp-1 Asn-34 PUBTATOR SpecificSite lamp-1 3916 Asn-34, Asn-93 Amino acid analysis and sequencing demonstrated that polylactosaminoglycans were located at Asn-34, Asn-93 and/or Asn-102, and Asn-195 and/or Asn-200 in lamp-1, and at Asn-4 and/or Asn-10, and Asn-279 in lamp-2. 3497198 7 29 gly glycosylation 972:984 arg1 H-2Kk H-2Kk PUBTATOR H-2Kk 14972 The glycosylation of H-2Kk did not vary between B10.A and C3H mice. 11912203 0 10 gly glycosylation 18:30 arg1 adiponectin adiponectin PUBTATOR adiponectin 9370 Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. 28165004 3 24 gly pembrolizumab/PD-1 418:435 arg1 the complex structure PD-1 the complex structure PUBTATOR PD-1 5133 However, the binding mechanism of nivolumab to PD-1 has not yet been shown, despite a recent report describing the complex structure of pembrolizumab/PD-1. 20581009 0 43 gly Endocan 85:91 arg1 a soluble endothelial proteoglycan Endocan a soluble endothelial proteoglycan PUBTATOR Endocan 11082 Characterization and binding activity of the chondroitin/dermatan sulfate chain from Endocan, a soluble endothelial proteoglycan. 17222411 2 47 part_of Thr52 299:303 arg1 human podoplanin podoplanin Thr52 OGER AminoAcid podoplanin Q86YL7 domain, and Thr52 Podoplanin possesses a platelet aggregation-stimulating (PLAG) domain, and Thr52 in the PLAG domain of human podoplanin is important for its activity. 17222411 2 48 part_of possesses 235:243 arg1 Podoplanin AND a platelet aggregation-stimulating (PLAG) domain Podoplanin a platelet aggregation-stimulating (PLAG) domain OGER AminoAcid Podoplanin Q86YL7 domain, and Thr52 Podoplanin possesses a platelet aggregation-stimulating (PLAG) domain, and Thr52 in the PLAG domain of human podoplanin is important for its activity. 28531887 11 45 part_of MT4-MMP 1449:1455 arg1 Asn318 MT4-MMP Asn318 PUBTATOR AminoAcid MT4-MMP 4326 Asn318 Moreover, we identified Asn318 as the single N-glycosylation site of MT4-MMP. 8323280 8 28 gly desialylated 1691:1702 arg1 desialylated rhLT desialylated rhLT Cterm rhLT The interaction between desialylated rhLT and uromodulin was inhibited by N,N'-diacetylchitobiose and [Man alpha 1-->6(Man alpha 1-->3)Man alpha 1-->6](Man alpha 1-->2Man alpha 1-->3)Man beta 1-->4GlcNAc beta 1-->4GlcNAc-->Asn. 1900431 0 23 gly has 29:31 arg1 Tissue plasminogen activator AND an O-linked fucose Tissue plasminogen activator an O-linked fucose PUBTATOR Tissue plasminogen activator P00750 Tissue plasminogen activator has an O-linked fucose attached to threonine-61 in the epidermal growth factor domain. 8687384 5 49 gly presence 984:991 arg1 Bowes t-PA AND glycans Bowes t-PA glycans PUBTATOR t-PA P00750 The presence on Bowes t-PA of glycans associated primarily with the nervous system is consistent with its expression in a cell line of neuroectodermal origin. 21402931 3 20 gly ZP 417:418 arg1 betaglycan Other ZP betaglycan Cterm Other ZP Other ZP proteins (namely, betaglycan and endoglin) do not polymerize but serve as important membrane coreceptors for ligands in the transforming growth factor-β (TGF-β) superfamily. 21402931 3 32 gly Other 411:415 arg1 betaglycan Other ZP betaglycan Cterm Other ZP Other ZP proteins (namely, betaglycan and endoglin) do not polymerize but serve as important membrane coreceptors for ligands in the transforming growth factor-β (TGF-β) superfamily. 2136357 1 47 gly glycoprotein 174:185 arg1 The urinary glycoprotein uromodulin The urinary glycoprotein uromodulin PUBTATOR uromodulin 7369 The urinary glycoprotein uromodulin (Tamm-Horsfall glycoprotein) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479-81; Hession et al., (1987) Science 237:1479-84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547-53]. 2136357 1 47 gly glycoprotein 174:185 arg1 Tamm-Horsfall glycoprotein Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 The urinary glycoprotein uromodulin (Tamm-Horsfall glycoprotein) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479-81; Hession et al., (1987) Science 237:1479-84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547-53]. 19299457 11 71 part_of TSHR 1461:1464 arg1 TSHR residues N198 and T200 TSHR residues N198 PUBTATOR SpecificSite TSHR 25360 residues N198 TSHR residues N198 and T200, like Y195, are on the convex facet of the leucine-rich domain. 7451505 0 31 gly ceruloplasmin 62:74 arg1 asparagine-linked sugar chains ceruloplasmin asparagine-linked sugar chains PUBTATOR ceruloplasmin 1356 Structural studies of asparagine-linked sugar chains of human ceruloplasmin. 22023369 2 33 gly nonfucosylated 409:422 arg1 nonfucosylated IgG1-Fc nonfucosylated IgG1-Fc OGER IgG1 P01857 Here, we present the 2.2-Å structure of the complex formed between nonfucosylated IgG1-Fc and a soluble form of FcγRIIIa (sFcγRIIIa) with two N-glycosylation sites. 15750791 5 23 gly N-glycan 769:776 arg1 Edg-1/S1P1 Edg-1 N-glycan PUBTATOR Edg-1 1901 These studies revealed a possible regulatory role for the N-glycan on Edg-1/S1P1 in the dynamics of the receptor, such as its lateral and internal movements within the membrane, in ligand-stimulated mammalian cells. 12096136 4 2 gly F-spondin 697:705 arg1 the thrombospondin type 1 repeats F-spondin the thrombospondin type 1 repeats PUBTATOR F-spondin 10418 Their analysis by a combined mass spectrometric approach is illustrated with peptides from the thrombospondin type 1 repeats (TSRs) of the recombinant axonal guidance protein F-spondin. 7592613 1 13 gly glycoprotein 270:281 arg1 Glycodelin Glycodelin PUBTATOR Glycodelin 5047 Glycodelin, also known as placental protein 14 (PP14) or progesterone-associated endometrial protein (PAEP), is a human glycoprotein with potent immunosuppressive and contraceptive activities. 26467158 3 16 gly modified 418:425 arg1 BACE1 AND GlcNAc BACE1 GlcNAc PUBTATOR BACE1 23821 We have recently found that BACE1 is modified with bisecting N-acetylglucosamine (GlcNAc) by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene) and that GnT-III deficiency reduces Aβ-plaque formation in the brain by accelerating lysosomal degradation of BACE1. 26467158 3 16 gly modified 418:425 arg3 BACE1 AND bisecting N-acetylglucosamine BACE1 bisecting N-acetylglucosamine PUBTATOR BACE1 23821 We have recently found that BACE1 is modified with bisecting N-acetylglucosamine (GlcNAc) by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene) and that GnT-III deficiency reduces Aβ-plaque formation in the brain by accelerating lysosomal degradation of BACE1. 2498325 3 57 gly apoE 269:272 arg1 The carbohydrate attachment site apoE The carbohydrate attachment site PUBTATOR apoE 348 The carbohydrate attachment site of plasma apoE was localized to a single tryptic peptide (residues 192-206). 23187000 7 23 gly glycosylation 1330:1342 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 Cancer cells appear to utilize these bilateral aspects of TIMP-1 for cancer progression; an elevated TIMP-1 level exerts to cancer development via MMP-independent pathway during the early phase of tumor formation, whereas it is the aberrant glycosylation of TIMP-1 that overcome the high anti-proteolytic burden. 8617200 4 2 gly LTBP-1 842:847 arg1 the third 8-Cys repeat LTBP-1 the third 8-Cys repeat PUBTATOR LTBP-1 4052 Immunoblotting of the fusion protein complexes indicated that the third 8-Cys repeat of LTBP-1 bound covalently to the LAP region of TGF-beta1. 2136357 2 20 gly derived 543:549 arg1 uromodulin AND the Man6(7)GlcNAc2-R glycopeptides uromodulin the Man6(7)GlcNAc2-R glycopeptides PUBTATOR uromodulin 7369 We report here that the Man6(7)GlcNAc2-R glycopeptides derived from uromodulin inhibit antigen-specific T cell proliferation by 50% at 0.2-2 microM, and further studies, reported elsewhere, confirm that oligomannose glycopeptides from other sources are also inhibitory, with Man9GlcNAc2-R the most inhibitory of those tested [Muchmore et al., J Leukocyte Biol (in press)]. 25081999 0 45 gly SCP1 42:45 arg1 In vivo putative O-GlcNAcylation SCP1 In vivo putative O-GlcNAcylation PUBTATOR SCP1 58190 In vivo putative O-GlcNAcylation of human SCP1 and evidence for possible role of its N-terminal disordered structure. 10731668 5 44 gly glycosylation 815:827 arg1 CHO sEGFR CHO sEGFR PUBTATOR CHO sEGFR P00533 The glycosylation at Asn(32 ) in CHO sEGFR was incomplete: 20% of Asn(32 ) remained unmodified. 7574684 2 54 gly contains 508:515 arg1 trkB AND 33.3% carbohydrate moieties trkB 33.3% carbohydrate moieties PUBTATOR trkB 4915 The extracellular domain contains 398 amino acids and has a molecular weight of 60.6 kDa according to laser desorption mass spectrometry, indicating that the extracellular domain of trkB contains 33.3% carbohydrate moieties. 8672508 6 30 part_of gp210 1109:1113 arg1 Ser1880 gp210 Ser1880 PUBTATOR AminoAcid gp210 23225 Ser1880 This analysis showed that Ser1880 of gp210 was phosphorylated in mitosis, possibly by cyclin B-p34cdc2 or a related kinase. 18203274 1 34 gly glycosylated 124:135 arg1 Human butyrylcholinesterase Human butyrylcholinesterase PUBTATOR Human butyrylcholinesterase 590 Human butyrylcholinesterase (hBChE) is a highly glycosylated protein present in human plasma. 7774715 0 20 gly isolated 49:56 arg2 recombinant human lactoferrin AND two N-linked glycans recombinant human lactoferrin two N-linked glycans OGER lactoferrin P02788 Structural determination of two N-linked glycans isolated from recombinant human lactoferrin expressed in BHK cells. 10756055 4 49 gly glycosylated 873:884 arg1 cell type-dependent glycosylated CXCR4 cell type-dependent glycosylated CXCR4 PUBTATOR CXCR4 7852 These results may have far-reaching implications for the differential recognition of cell type-dependent glycosylated CXCR4 by HIV-1 isolates and their evolution in vivo. 7514386 10 67 gly U-CD59 1346:1351 arg1 the asparagine-linked sugar chains CD59 the asparagine-linked sugar chains PUBTATOR CD59 966 The structures of the asparagine-linked sugar chains of U-CD59 were biantennary complex type, only 4.2% of which are monosialylated. 25458834 0 11 gly O-glycosylated 61:74 arg1 O-glycosylated podoplanin O-glycosylated podoplanin OGER podoplanin Q86YL7 A platform of C-type lectin-like receptor CLEC-2 for binding O-glycosylated podoplanin and nonglycosylated rhodocytin. 479158 6 23 gly contains 394:401 arg1 Plasminogen variant 1 AND an asparagine288-based branched carbohydrate structure Plasminogen variant 1 an asparagine288-based branched carbohydrate structure OGER Plasminogen variant 1 P00747 Plasminogen variant 1 contains an asparagine288-based branched carbohydrate structure, which has been established in the immediately preceding manuscript. 25802287 9 34 gly glycosylation 1442:1454 arg1 human serum IgM human serum IgM OGER IgM P01871 We applied the microarray approach to a detailed site-specific glycosylation analysis of human serum IgM. 2963625 5 40 gly H 720:720 arg1 the seventh homologous repeat unit factor H the seventh homologous repeat unit PUBTATOR factor H 3075 A tyrosine/histidine polymorphism was observed within the seventh homologous repeat unit of factor H. 22556278 3 84 gly O-Glycosylation 387:401 arg1 the 26 S proteasome ATPase subunit Rpt2 the 26 S proteasome ATPase subunit Rpt2 OGER 26 S proteasome O-Glycosylation of the 26 S proteasome ATPase subunit Rpt2 is known to influence the stability of proteins by reducing their proteasome-dependent degradation. 9572875 3 14 gly glycosylated 659:670 arg1 glycosylated IGFBP-6 glycosylated IGFBP-6 PUBTATOR IGFBP-6 3489 Electrospray ionization mass spectrometry (ESMS) of glycosylated IGFBP-6 revealed considerable heterogeneity of carbohydrate composition. 18930737 0 14 gly alpha2-glycoprotein 59:77 arg1 ZAG ZAG PUBTATOR ZAG 563 Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma. 18930737 0 14 gly alpha2-glycoprotein 59:77 arg1 zinc alpha2-glycoprotein zinc alpha2-glycoprotein PUBTATOR zinc alpha2-glycoprotein 563 Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma. 19683538 6 24 gly glycoprotein 1071:1082 arg1 the alpha-NAGAL glycoprotein the alpha-NAGAL glycoprotein Cterm alpha-NAGAL glycoprotein 4668 To better understand how individual defects in the alpha-NAGAL glycoprotein lead to Schindler disease, we analyzed the effect of disease-causing mutations on the three-dimensional structure. 8407880 6 51 gly HGF 1030:1032 arg1 the N-linked oligosaccharides HGF the N-linked oligosaccharides PUBTATOR HGF 24446 The structures of the N-linked oligosaccharides from rat HGF were also studied. 9295302 6 39 gly deglycosylation 887:901 arg1 intact MRP intact MRP PUBTATOR MRP 4363 Limited proteolysis of MRP-enriched membranes and deglycosylation of intact MRP and its tryptic fragments with PNGase F was carried out followed by immunoblotting with antibodies known to react with specific regions of MRP. 23139753 8 6 gly N-glycosylated 886:899 arg1 HE-4 HE-4 PUBTATOR HE-4 10406 Moreover, HE-4 is N-glycosylated and highly stable on a wide range of pH and temperature. 22159084 12 65 gly deglycosylated 1521:1534 arg1 deglycosylated SLC26A3 deglycosylated SLC26A3 PUBTATOR SLC26A3 1811 While the mature glycosylated SLC26A3 showed little breakdown after treatment with trypsin, deglycosylated SLC26A3 exhibited increased susceptibility to trypsin, suggesting that the oligosaccharides protect SLC26A3 from tryptic digestion. 22159084 12 68 gly glycosylated 1446:1457 arg1 the mature glycosylated SLC26A3 the mature glycosylated SLC26A3 PUBTATOR SLC26A3 1811 While the mature glycosylated SLC26A3 showed little breakdown after treatment with trypsin, deglycosylated SLC26A3 exhibited increased susceptibility to trypsin, suggesting that the oligosaccharides protect SLC26A3 from tryptic digestion. 20356926 6 29 gly glycosylation 633:645 arg1 CREB-H CREB-H PUBTATOR CREB-H 84699 Disruption of all three sites by site-directed mutagenesis completely abrogated N-linked glycosylation of CREB-H. 10677208 0 30 gly N-glycosylation 55:69 arg1 LTBP-1 LTBP-1 OGER LTBP-1 Q14766 Hybrid and complex glycans are linked to the conserved N-glycosylation site of the third eight-cysteine domain of LTBP-1 in insect cells. 7642555 9 10 part_of c-Myc 1120:1124 arg1 threonine 58 c-Myc threonine 58 PUBTATOR SpecificSite c-Myc 4609 threonine 58 These analyses show that threonine 58, an in vivo phosphorylation site in the transactivation domain, is the major O-GlcNAc glycosylation site of c-Myc. 27922006 0 49 gly glycoprotein 38:49 arg1 myelin-associated glycoprotein adhesion myelin-associated glycoprotein adhesion PUBTATOR myelin-associated glycoprotein 4099 Structural basis of myelin-associated glycoprotein adhesion and signalling. 8636209 12 50 gly glycosylation 1531:1543 arg1 CD3 delta CD3 delta PUBTATOR CD3 delta 915 Furthermore, the study indicated that, in contrast to CD3 gamma, glycosylation of CD3 delta is required for TCR assembly and expression. 8636209 12 50 gly glycosylation 1531:1543 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 Furthermore, the study indicated that, in contrast to CD3 gamma, glycosylation of CD3 delta is required for TCR assembly and expression. 2390069 4 7 gly released 636:643 arg1 human leucocyte lactotransferrin AND oligosaccharide alditols human leucocyte lactotransferrin oligosaccharide alditols PUBTATOR lactotransferrin 4057 Structural analysis combining methylation-mass spectrometry and 400 MHz 1H-n.m.r. spectrometry of oligosaccharide alditols released from human leucocyte lactotransferrin shows the presence of two disialylated and non-fucosylated biantennary glycans of the N-acetyl-lactosaminic type. 15454184 0 57 gly glycosylation 26:38 arg1 recombinant human follistatin recombinant human follistatin PUBTATOR follistatin 10468 Analysis of site-specific glycosylation in recombinant human follistatin expressed in Chinese hamster ovary cells. 7430095 0 5 gly A 79:79 arg1 the asparagine-linked sugar chains Glycophorin A the asparagine-linked sugar chains PUBTATOR Glycophorin A 2993 Structures of the asparagine-linked sugar chains of glycophorin A. Glycophorin A isolated from human erythrocytes contains one asparagine-linked sugar chain in one molecule. 2547792 2 63 part_of alpha-MSH 263:271 arg1 D-phenylalanine7 MSH D-phenylalanine7 PUBTATOR AminoAcid MSH 17701 phenylalanine7 The melanotropin (MSH) receptor of mouse B16-F1 melanoma cells was characterized by photoaffinity cross-linking, using a potent alpha-MSH photolabel, [norleucine4, D-phenylalanine7, 1'-(2-nitro-4-azidophenylsulfenyl)-tryptophan9]-alpha-melanotropin (Naps-MSH). 3840370 13 44 gly 2-glycoprotein 1955:1968 arg1 the non-complement beta 2-glycoprotein I the non-complement beta 2-glycoprotein I OGER beta 2-glycoprotein I P02749 These regions in C4b-binding protein are homologous with the three internal-homology regions that have been reported to be present within the Ba region of the complement enzyme factor B and also to the internal-homology regions found in the non-complement beta 2-glycoprotein I. 3427055 0 84 gly glycoprotein 115:126 arg1 SPARC/BM-40/osteonectin SPARC/BM-40/osteonectin PUBTATOR BM-40 6678 Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues. 16877748 1 4 gly N-glycosylated 204:217 arg1 Intercellular adhesion molecule-1 Intercellular adhesion molecule-1 PUBTATOR Intercellular adhesion molecule-1 15894 Intercellular adhesion molecule-1 (ICAM-1) is a heavily N-glycosylated transmembrane protein comprising five extracellular Ig-like domains. 7925474 3 78 gly glycoprotein 402:413 arg1 FA1 FA1 PUBTATOR FA1 8788 FA1 is a single-chained, heterogeneous glycoprotein of 225-262 amino acid residues. 21500857 8 14 gly AP180 988:992 arg1 GlcNAc-P AP180 GlcNAc-P PUBTATOR AP180 65178 Analysis of synthetic GlcNAc-6-P produced identical fragmentation products to GlcNAc-P from AP180. 24334224 2 54 gly glycosylation 489:501 arg1 rhG-CSF rhG-CSF OGER CSF Using liquid chromatography and tandem mass spectrometry techniques, we analyzed the O-linked glycosylation of recombinant human granulocyte colony-stimulating factor (rhG-CSF) derived from glycoengineered Pichia pastoris with regard to its nature, structure, occupancy, and location. 24334224 2 54 gly glycosylation 489:501 arg1 recombinant human granulocyte colony-stimulating factor recombinant human granulocyte colony-stimulating factor PUBTATOR granulocyte colony-stimulating factor 1440 Using liquid chromatography and tandem mass spectrometry techniques, we analyzed the O-linked glycosylation of recombinant human granulocyte colony-stimulating factor (rhG-CSF) derived from glycoengineered Pichia pastoris with regard to its nature, structure, occupancy, and location. 22159084 9 53 gly glycosylated 1211:1222 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 Immnoblotting suggests that SLC26A3 is glycosylated at N153, N161, and N165. 9578468 0 41 gly O-glycosylated 42:55 arg1 human eotaxin human eotaxin PUBTATOR eotaxin 6356 Delayed production of biologically active O-glycosylated forms of human eotaxin by tumor-necrosis-factor-alpha-stimulated dermal fibroblasts. 11683872 6 31 gly glycosylated 829:840 arg1 This mutated VR1 This mutated VR1 PUBTATOR VR1 7442 This mutated VR1 was not glycosylated, confirming the extracellular location of N604 and its role as the exclusive site of glycosylation of the VR1 protein. 11683872 6 49 gly glycosylation 927:939 arg1 the VR1 protein the VR1 protein PUBTATOR VR1 protein 7442 This mutated VR1 was not glycosylated, confirming the extracellular location of N604 and its role as the exclusive site of glycosylation of the VR1 protein. 11459848 7 13 gly N-glycans 821:829 arg1 recombinant BSP BSP N-glycans PUBTATOR BSP 3381 Carbohydrate analysis revealed 10 different complex-type N-glycans on both proteins and eight different O-glycans on recombinant BSP, four of those were found on bone-derived BSP. 11459848 7 60 gly O-glycans 868:876 arg1 recombinant BSP BSP O-glycans PUBTATOR BSP 3381 Carbohydrate analysis revealed 10 different complex-type N-glycans on both proteins and eight different O-glycans on recombinant BSP, four of those were found on bone-derived BSP. 2059624 1 95 part_of plasminogen 182:192 arg1 asparagine-289 plasminogen asparagine-289 OGER SpecificSite plasminogen P00747 asparagine-289 Temporal dependence of the nature of the oligosaccharides assembled on asparagine-289 of recombinant human plasminogen produced in baculovirus vector infected Spodoptera frugiperda (IPLB-SF-21AE) cells. 1482348 1 18 gly HGF 185:187 arg1 O-glycosylated oligosaccharide HGF O-glycosylated oligosaccharide PUBTATOR HGF 3082 The glycosylation site and the structure of O-glycosylated oligosaccharide of recombinant human HGF were investigated. 11567096 1 33 gly glycosylated 346:357 arg1 Rat corticotropin-releasing factor receptor 1 Rat corticotropin-releasing factor receptor 1 PUBTATOR corticotropin-releasing factor receptor 1 81648 Rat corticotropin-releasing factor receptor 1 (rCRFR1) was produced either in transfected HEK 293 cells as a complex glycosylated protein or in the presence of the mannosidase I inhibitor kifunensine as a high mannose glycosylated protein. 11567096 1 64 gly corticotropin-releasing 132:154 arg1 a high mannose glycosylated protein corticotropin-releasing factor receptor 1 a high mannose glycosylated protein PUBTATOR corticotropin-releasing factor receptor 1 81648 Rat corticotropin-releasing factor receptor 1 (rCRFR1) was produced either in transfected HEK 293 cells as a complex glycosylated protein or in the presence of the mannosidase I inhibitor kifunensine as a high mannose glycosylated protein. 11567096 1 67 gly factor 156:161 arg1 a high mannose glycosylated protein corticotropin-releasing factor receptor 1 a high mannose glycosylated protein PUBTATOR corticotropin-releasing factor receptor 1 81648 Rat corticotropin-releasing factor receptor 1 (rCRFR1) was produced either in transfected HEK 293 cells as a complex glycosylated protein or in the presence of the mannosidase I inhibitor kifunensine as a high mannose glycosylated protein. 23187000 3 27 gly glycoprotein 540:551 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 Among TIMPs, TIMP-1, a 184-residue protein, is the only N-linked glycoprotein with glycosylation sites at N30 and N78. 8617200 0 29 gly LTBP-1 117:122 arg1 an eight cysteine repeat LTBP-1 an eight cysteine repeat PUBTATOR LTBP-1 4052 Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1. 11098061 0 72 gly N-glycosylation 45:59 arg1 the human signal transducer gp130 the human signal transducer gp130 PUBTATOR gp130 P40189 Determination of the disulfide structure and N-glycosylation sites of the extracellular domain of the human signal transducer gp130. 2775174 9 35 gly N-glycosylation 1118:1132 arg1 the same subunit subunit OGER subunit P20933 N-Terminal sequence analysis of each subunit revealed a frayed N-terminus of the 24 kDa subunit and an apparent N-glycosylation of Asn-15 in the same subunit. 9450956 0 62 gly C-mannosylation 23:37 arg1 RNase 2 RNase 2 C-mannosylation PUBTATOR RNase 2 6036 Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp. 10988252 1 16 gly glycoprotein 181:192 arg1 The human epidermal growth factor receptor The human epidermal growth factor receptor PUBTATOR epidermal growth factor receptor 1956 The human epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein having 11 potential N-glycosylation sites in its extracellular domain. 15014436 9 43 gly TSP-5/COMP 1091:1100 arg1 the T3 repeats TSP the T3 repeats PUBTATOR TSP P07996 Mutations in the T3 repeats of TSP-5/COMP, which cause two human skeletal disorders, are predicted to disrupt the tertiary structure of the T3-CTD assembly. 22226965 4 7 gly PPARγ 641:645 arg1 the major O-GlcNAc site PPARγ the major O-GlcNAc site PUBTATOR PPARγ 19016 Mass spectrometric analysis and mutant studies revealed that the threonine 54 of the N-terminal AF-1 domain of PPARγ is the major O-GlcNAc site. 22226965 4 50 gly AF-1 626:629 arg1 the major O-GlcNAc site AF-1 the major O-GlcNAc site OGER AF-1 P38484 Mass spectrometric analysis and mutant studies revealed that the threonine 54 of the N-terminal AF-1 domain of PPARγ is the major O-GlcNAc site. 23187000 8 37 gly glycosylation 1415:1427 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 The aberrant glycosylation of TIMP-1 can thus be used as staging and/or prognostic biomarker in colon cancer. 12218058 2 38 part_of has 375:377 arg1 BMP-1 AND Asn(91) BMP-1 Asn(91) PUBTATOR SpecificSite BMP-1 649 Asn(91) Sequence analysis suggests that BMP-1 has six potential N-linked glycosylation sites (i.e. NXS/T) namely: Asn(91) (prodomain), Asn(142) (metalloproteinase domain), Asn(332) and Asn(363) (CUB1 domain), Asn(599) (CUB3 domain), and Asn(726) in the C-terminal-specific domain. 12218058 2 38 part_of has 375:377 arg1 BMP-1 AND Asn(599) BMP-1 Asn(599) PUBTATOR SpecificSite BMP-1 649 Asn(599) Sequence analysis suggests that BMP-1 has six potential N-linked glycosylation sites (i.e. NXS/T) namely: Asn(91) (prodomain), Asn(142) (metalloproteinase domain), Asn(332) and Asn(363) (CUB1 domain), Asn(599) (CUB3 domain), and Asn(726) in the C-terminal-specific domain. 12218058 2 38 part_of has 375:377 arg1 BMP-1 AND Asn(142) BMP-1 Asn(142) PUBTATOR SpecificSite BMP-1 649 Asn(142) Sequence analysis suggests that BMP-1 has six potential N-linked glycosylation sites (i.e. NXS/T) namely: Asn(91) (prodomain), Asn(142) (metalloproteinase domain), Asn(332) and Asn(363) (CUB1 domain), Asn(599) (CUB3 domain), and Asn(726) in the C-terminal-specific domain. 12218058 2 38 part_of has 375:377 arg1 BMP-1 AND Asn(332) BMP-1 Asn(332) PUBTATOR SpecificSite BMP-1 649 Asn(332) and Asn(363) Sequence analysis suggests that BMP-1 has six potential N-linked glycosylation sites (i.e. NXS/T) namely: Asn(91) (prodomain), Asn(142) (metalloproteinase domain), Asn(332) and Asn(363) (CUB1 domain), Asn(599) (CUB3 domain), and Asn(726) in the C-terminal-specific domain. 12022871 8 4 gly glycosylated 1241:1252 arg1 Human TGH Human TGH PUBTATOR Human TGH 1066 Human TGH was glycosylated in the insect cells. 15807535 8 66 gly glycosylated 1513:1524 arg1 glycosylated ABCG2 glycosylated ABCG2 PUBTATOR ABCG2 Q9UNQ0 The ABCG2 (N596Q) variant is also functional, demonstrating rhodamine 123 transport in intact cells comparable to that in cells expressing glycosylated ABCG2. 8068684 7 84 gly contains 1270:1277 arg1 NHE-1 AND O-linked oligosaccharide NHE-1 O-linked oligosaccharide PUBTATOR NHE-1 6548 In addition, treatment of NHE-1 with neuraminidase and O-glycosidase demonstrated that NHE-1 also contains O-linked oligosaccharide. 25187573 9 32 gly O-glycosylation 1717:1731 arg1 lubricin lubricin PUBTATOR lubricin 10216 This suggests that there is a unique combination of transferase genes important for the O-glycosylation of lubricin. 1421756 8 46 gly present 1303:1309 arg1 the TfR AND only one O-linked oligosaccharide TfR only one O-linked oligosaccharide PUBTATOR TfR 7037 These and other results demonstrate that only one O-linked oligosaccharide is present in the TfR and that it occurs on either Thr96 or Thr104. 18491227 9 20 gly N-glycosylation 1457:1471 arg1 E-cadherin expression E-cadherin expression PUBTATOR E-cadherin 999 In conclusion, this study revealed that N-glycosylation at Asn-633 is essential for E-cadherin expression, folding and trafficking. 9169007 1 3 gly glycoforms 166:175 arg1 recombinant antithrombin recombinant antithrombin PUBTATOR antithrombin 462 Two major glycoforms of recombinant antithrombin which differ 10-fold in their affinity for the effector glycosaminoglycan, heparin, were previously shown to be expressed in BHK or CHO mammalian cell lines (I. Björk, et al., 1992, Biochem. 17040911 6 33 gly glycans 1021:1027 arg1 Asn-270 Asn-270 Asn-270 SpecificSite Asn-234 and Asn-270 The removal of two glycans in the protease domain at Asn-234 and Asn-270, as well as one in the tumor necrosis factor receptor-associated factor domain at Asn-452, by a deglycosidase under nondenaturing conditions decreased the chemical and thermal stability of the homo-oligomer without affecting quaternary structure. 17040911 6 33 gly glycans 1021:1027 arg1 Asn-234 Asn-234 Asn-234 SpecificSite Asn-234 and Asn-270 The removal of two glycans in the protease domain at Asn-234 and Asn-270, as well as one in the tumor necrosis factor receptor-associated factor domain at Asn-452, by a deglycosidase under nondenaturing conditions decreased the chemical and thermal stability of the homo-oligomer without affecting quaternary structure. 9054441 6 3 gly glycosylation 952:964 arg1 plasminogen 2 plasminogen 2 OGER plasminogen 2 P00747 In the present study, a combination of trypsin digestion, lectin affinity chromatography, Edman degradation amino acid sequence analysis, carbohydrate composition analysis, and mass spectrometry revealed the existence of a novel site for O-linked glycosylation on plasminogen 2 at Ser-248. 12839991 3 45 gly NgR 568:570 arg1 the entire leucine-rich repeat (LRR) region NgR the entire leucine-rich repeat (LRR) region OGER NgR Q9BZR6 By deletion analysis, we show that the binding of soluble fragments of Nogo, MAG and NgR to cell-surface NgR requires the entire leucine-rich repeat (LRR) region of NgR, but not other portions of the protein. 16847056 3 75 gly beta2 411:415 arg1 essentially no sialylation beta2 essentially no sialylation Cterm beta2 To determine whether beta2-linked sialic acids similarly impact Nav gating, we co-expressed beta2 with Nav1.5 or Nav1.2 in Pro5 (complete sialylation) and in Lec2 (essentially no sialylation) cells. 16847056 3 75 gly beta2 411:415 arg1 complete sialylation beta2 complete sialylation Cterm beta2 To determine whether beta2-linked sialic acids similarly impact Nav gating, we co-expressed beta2 with Nav1.5 or Nav1.2 in Pro5 (complete sialylation) and in Lec2 (essentially no sialylation) cells. 16847056 3 75 gly beta2 411:415 arg1 Lec2 (essentially no sialylation) cells beta2 Lec2 (essentially no sialylation) cells Cterm beta2 To determine whether beta2-linked sialic acids similarly impact Nav gating, we co-expressed beta2 with Nav1.5 or Nav1.2 in Pro5 (complete sialylation) and in Lec2 (essentially no sialylation) cells. 3881423 7 42 gly glycoproteins 1259:1271 arg1 neutral alpha-glucosidase AB neutral alpha-glucosidase AB PUBTATOR neutral alpha-glucosidase AB 23193 Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 3881423 7 42 gly glycoproteins 1259:1271 arg1 glucosidase II glucosidase II OGER glucosidase II Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 3881423 7 48 gly have 1341:1344 arg1 neutral alpha-glucosidase AB AND the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside neutral alpha-glucosidase AB the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside PUBTATOR neutral alpha-glucosidase AB 23193 Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 3881423 7 48 gly have 1341:1344 arg1 glucosidase II AND the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside glucosidase II the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside OGER glucosidase II Both glucosidase II and neutral alpha-glucosidase AB are high-molecular mass (greater than 200,000 dalton) anionic glycoproteins which bind to concanavalin A, have a broad pH optima (5.5-8.5), and have a similar Km for maltose (4.8 versus 2.1 mM) and the artificial substrate 4-methylumbelliferyl-alpha-D-glucopyranoside (35 versus 19 microM). 28450392 4 7 part_of histone 874:880 arg1 histone H4 residues Lys-16, Lys-5, and Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 7 part_of histone 874:880 arg1 Lys-5 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 7 part_of histone 874:880 arg1 Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 7 part_of histone 874:880 arg1 Lys-5 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 7 part_of histone 874:880 arg1 Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 7 part_of histone 874:880 arg1 Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 57 part_of H4 882:883 arg1 histone H4 residues Lys-16, Lys-5, and Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 57 part_of H4 882:883 arg1 Lys-5 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 57 part_of H4 882:883 arg1 Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 57 part_of H4 882:883 arg1 Lys-5 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 57 part_of H4 882:883 arg1 Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 28450392 4 57 part_of H4 882:883 arg1 Lys-8 histone H4 residues Lys-16, Lys-5, and Lys-8 OGER SpecificSite histone H4 P62805 residues Lys-16, Lys-5, and Lys-8 Knocking down or overexpressing OGT1 in human cells remarkably affected the global acetylation of histone H4 residues Lys-16, Lys-5, and Lys-8. 19478079 4 26 gly modified 613:620 arg1 C/EBPbeta AND a dynamic single sugar modification C/EBPbeta a dynamic single sugar modification PUBTATOR C/EBPbeta 1051 Here we show that C/EBPbeta is modified by O-GlcNAc, a dynamic single sugar modification found on nucleocytoplasmic proteins. 19478079 4 26 gly modified 613:620 arg1 C/EBPbeta AND O-GlcNAc C/EBPbeta O-GlcNAc PUBTATOR C/EBPbeta 1051 Here we show that C/EBPbeta is modified by O-GlcNAc, a dynamic single sugar modification found on nucleocytoplasmic proteins. 22387313 3 52 gly glycoprotein 484:495 arg1 DPP10 DPP10 PUBTATOR DPP10 57628 DPP10 is a glycoprotein containing eight predicted N-glycosylation sites in the extracellular domain. 17980170 0 42 gly glycosylation 27:39 arg1 N-acylethanolamine-hydrolyzing acid amidase N-acylethanolamine-hydrolyzing acid amidase PUBTATOR N-acylethanolamine-hydrolyzing acid amidase 27163 Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism. 21308849 3 29 gly glycoprotein 516:527 arg1 MAG MAG PUBTATOR MAG 4099 It has been shown by others that the LRR and stalk regions of NgR1 and NgR2 have distinct roles in conferring binding affinity to the myelin associated glycoprotein (MAG) in vivo. 21308849 3 29 gly glycoprotein 516:527 arg1 the myelin associated glycoprotein the myelin associated glycoprotein PUBTATOR myelin associated glycoprotein 4099 It has been shown by others that the LRR and stalk regions of NgR1 and NgR2 have distinct roles in conferring binding affinity to the myelin associated glycoprotein (MAG) in vivo. 25765764 1 42 gly LRIG1-ECD 129:137 arg1 the LRIG1-LRR (leucine-rich repeat) domain LRIG1 the LRIG1-LRR (leucine-rich repeat) domain PUBTATOR LRIG1 26018 We have expressed and purified three soluble fragments of the human LRIG1-ECD (extracellular domain): the LRIG1-LRR (leucine-rich repeat) domain, the LRIG1-3Ig (immunoglobulin-like) domain, and the LRIG1-LRR-1Ig fragment using baculovirus vectors in insect cells. 8477709 0 52 gly erythropoietin 51:64 arg1 sialylated oligosaccharides erythropoietin sialylated oligosaccharides PUBTATOR erythropoietin 2056 Structures of sialylated oligosaccharides of human erythropoietin expressed in recombinant BHK-21 cells. 10764840 3 44 gly rHuEpo 542:547 arg1 the glycan chains rHuEpo the glycan chains Cterm rHuEpo 2056 The structure of the glycan chains of this rHuEpo slightly differ of those of the urinary human Epo (uHuEpo), considered as the natural Epo molecule. 15807535 10 102 gly N-glycosylation 1843:1857 arg1 ABCG2 ABCG2 PUBTATOR ABCG2 Q9UNQ0 Although subtle defects in transporter trafficking and function may exist, these data taken together suggest that N-glycosylation at arginine 596 is not essential for the expression, trafficking to the plasma membrane, or the overall function of ABCG2. 25081999 5 50 part_of hSCP1 1014:1018 arg1 the Ser41 residue hSCP1 the Ser41 residue PUBTATOR AminoAcid hSCP1 58190 Ser41 residue To gain insight into the PTM of hSCP1, we used the Western blot, immunoprecipitation, succinylayed wheat germ agglutininprecipitation, liquid chromatography-mass spectrometry analyses, and site-directed mutagenesis and identified the Ser41 residue of hSCP1 as the O-GlcNAc modification site. 7694285 2 76 gly [CGT 334:337 arg1 UDPgalactose UDPgalactosyltransferase [CGT UDPgalactose PUBTATOR UDPgalactosyltransferase [CGT 7368 Cerebrosides are synthesized by ceramide UDPgalactosyltransferase [CGT; 2-hydroxyacylsphinogosine 1-beta-galactosyl-transferase; UDPgalactose:2-(2-hydroxyacyl)sphingosine 1-beta-D-galactosyltransferase; UDPgalactose:2-(2-hydroxyacyl)sphingosine 1-beta-D-galactosyltransferase, EC 2.4.1.45] with UDPgalactose and ceramide as substrates. 22226965 0 28 gly PPARγ 25:29 arg1 O-GlcNAc modification PPARγ O-GlcNAc modification PUBTATOR PPARγ 19016 O-GlcNAc modification of PPARγ reduces its transcriptional activity. 14764083 6 63 part_of containing 1011:1020 arg1 rhLF AND Thr130 rhLF Thr130 and Cys404 OGER AminoAcid rhLF P02788 Thr130 and Cys404 J. 312, 107-114] is restricted to rhLF containing the Thr130 and Cys404. 17293352 6 104 gly GAA 667:669 arg1 The N-linked glycans GAA The N-linked glycans PUBTATOR GAA 2548 The N-linked glycans of recombinant human GAA (rhAGLU), isolated from the rabbit milk, were released by peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase F. 15536627 0 42 gly transferrin 46:56 arg1 Site-specific carbohydrate profiling transferrin Site-specific carbohydrate profiling PUBTATOR transferrin 7018 Site-specific carbohydrate profiling of human transferrin by nano-flow liquid chromatography/electrospray ionization mass spectrometry. 8672508 3 44 gly modified 550:557 arg3 Nup214 AND O-linked N-acetylglucosamine Nup214 O-linked N-acetylglucosamine PUBTATOR Nup214 8021 Nonmembrane nucleoporins Nup153, Nup214, and Nup358 that are modified by O-linked N-acetylglucosamine and recognized by a monoclonal antibody were phosphorylated throughout the cell cycle and hyperphosphorylated during M phase. 8672508 3 44 gly modified 550:557 arg3 Nup358 AND O-linked N-acetylglucosamine Nup358 O-linked N-acetylglucosamine PUBTATOR Nup358 5903 Nonmembrane nucleoporins Nup153, Nup214, and Nup358 that are modified by O-linked N-acetylglucosamine and recognized by a monoclonal antibody were phosphorylated throughout the cell cycle and hyperphosphorylated during M phase. 8672508 3 44 gly modified 550:557 arg3 Nup153 AND O-linked N-acetylglucosamine Nup153 O-linked N-acetylglucosamine PUBTATOR Nup153 9972 Nonmembrane nucleoporins Nup153, Nup214, and Nup358 that are modified by O-linked N-acetylglucosamine and recognized by a monoclonal antibody were phosphorylated throughout the cell cycle and hyperphosphorylated during M phase. 25153361 5 27 gly glycoproteins 801:813 arg1 human alpha-1-acid glycoprotein human alpha-1-acid glycoprotein OGER glycoprotein (5 P40197 We demonstrated the effectiveness of the system using a set of glycoproteins including human transferrin (2 sequons), human alpha-1-acid glycoprotein (5 sequons), and influenza A virus hemagglutinin (9 sequons). 25153361 5 27 gly glycoproteins 801:813 arg1 human transferrin human transferrin PUBTATOR transferrin 7018 We demonstrated the effectiveness of the system using a set of glycoproteins including human transferrin (2 sequons), human alpha-1-acid glycoprotein (5 sequons), and influenza A virus hemagglutinin (9 sequons). 25153361 5 55 gly glycoprotein 875:886 arg1 human alpha-1-acid glycoprotein human alpha-1-acid glycoprotein OGER glycoprotein (5 P40197 We demonstrated the effectiveness of the system using a set of glycoproteins including human transferrin (2 sequons), human alpha-1-acid glycoprotein (5 sequons), and influenza A virus hemagglutinin (9 sequons). 20188224 3 46 gly glycoprotein 389:400 arg1 PGRN PGRN PUBTATOR PGRN 2896 PGRN is a glycoprotein, containing five N-glycosylation consensus sequons, three of which fall within granulin domains. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND galactose the tumor necrosis factor-alpha galactose PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND N-acetylneuraminic acid the tumor necrosis factor-alpha N-acetylneuraminic acid PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND N-acetylgalactosamine the tumor necrosis factor-alpha N-acetylgalactosamine PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND sugar components the tumor necrosis factor-alpha sugar components PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 16847056 13 64 gly beta2 1583:1587 arg1 only the sialic acid-independent depolarizing effects beta2 only the sialic acid-independent depolarizing effects Cterm beta2 When sialylation was reduced, only the sialic acid-independent depolarizing effects of beta2 on Nav1.2 gating were apparent. 1567356 11 28 gly glycosylation 1641:1653 arg1 OMD OMD OGER OMD Q99983 The results indicate that the 'site-directed' model of processing offers the most consistent explanation for the structures seen at the individual glycosylation sites of OMD. 21932778 8 64 gly N-deglycosylated 1374:1389 arg1 N-deglycosylated glypican-1 N-deglycosylated glypican-1 PUBTATOR N-deglycosylated glypican-1 2817 A single unfolding transition at high concentrations of urea was found for both N-deglycosylated glypican-1 and glypican-1 in which the N-glycosylation sites had been removed by mutagenesis when chemical denaturation was monitored by circular dichroism and fluorescence emission spectroscopy. 17286803 5 39 gly non-glycosylated 585:600 arg1 Both partially and non-glycosylated CLN3 Both partially and non-glycosylated CLN3 OGER CLN3 Q13286 Both partially and non-glycosylated CLN3 were transported correctly to lysosomes. 9450956 5 36 gly nonglycosylated 561:575 arg1 nonglycosylated RNase 4 nonglycosylated RNase 4 PUBTATOR RNase 4 6038 Expression of chimeras of RNase 2 and nonglycosylated RNase 4 and deletion mutants in HEK293 cells identified residues 1-13 to be sufficient for C-mannosylation. 7309709 0 1 gly prothrombin 62:72 arg1 the carbohydrate moiety prothrombin the carbohydrate moiety PUBTATOR prothrombin 2147 Studies on the structures of the carbohydrate moiety of human prothrombin. 2943741 4 21 gly Gal 750:752 arg1 NeuNAc alpha 2----6GalNAcOH, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH GalNAcOH, Gal beta NeuNAc alpha 2----6GalNAcOH, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH OGER GalNAcOH, Gal beta Q8N6F7 Oligosaccharides from K562 cells were found to be GalNAcOH, Gal beta 1----3GalNAcOH, NeuNAc alpha 2----6GalNAcOH, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH. 2943741 4 74 gly beta 754:757 arg1 NeuNAc alpha 2----6GalNAcOH, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH GalNAcOH, Gal beta NeuNAc alpha 2----6GalNAcOH, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH OGER GalNAcOH, Gal beta Q8N6F7 Oligosaccharides from K562 cells were found to be GalNAcOH, Gal beta 1----3GalNAcOH, NeuNAc alpha 2----6GalNAcOH, NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----3(NeuNAc alpha 2----6)GalNAcOH. 26828122 5 1 gly Glycosylation 967:979 arg1 hTPPT hTPPT PUBTATOR hTPPT 80736 Glycosylation of hTPPT was shown, by mean of site-directed mutagenesis, to occur at Asn(69), Asn(155), Asn(197), Asn(393), and Asn(416). 20356926 8 6 gly deglycosylated 871:884 arg1 unglycosylated or deglycosylated CREB-H unglycosylated or deglycosylated CREB-H PUBTATOR CREB-H 84699 Upon stimulation with an activator of intramembrane proteolysis such as brefeldin A and KDEL-tailed site 1 protease, unglycosylated or deglycosylated CREB-H was largely uncleaved, retained in an inactive form in the endoplasmic reticulum, and less capable of activating transcription driven by unfolded protein response element or C-reactive protein promoter. 20356926 8 51 gly unglycosylated 853:866 arg1 unglycosylated or deglycosylated CREB-H unglycosylated or deglycosylated CREB-H PUBTATOR CREB-H 84699 Upon stimulation with an activator of intramembrane proteolysis such as brefeldin A and KDEL-tailed site 1 protease, unglycosylated or deglycosylated CREB-H was largely uncleaved, retained in an inactive form in the endoplasmic reticulum, and less capable of activating transcription driven by unfolded protein response element or C-reactive protein promoter. 18703501 7 25 part_of contains 1209:1216 arg1 CA IX AND Asn(309) CA IX Asn(309) PUBTATOR SpecificSite CA IX 768 Asn(309) Mass spectrometry experiments showed that CA IX contains an intramolecular disulfide bridge (Cys(119)-Cys(299)) and a unique N-linked glycosylation site (Asn(309)) that bears high mannose-type glycan structures. 1694179 9 28 gly glycosylated 1241:1252 arg1 beta protein C beta protein C Cterm beta protein C It is asparagine 329 that is not glycosylated in beta protein C since antibodies to a synthetic peptide based on the sequence around this amino acid react only with beta protein C. 6619126 0 78 gly glycophorin 54:64 arg1 A carbohydrate structural variant glycophorin A A carbohydrate structural variant PUBTATOR glycophorin A 2993 A carbohydrate structural variant of MM glycoprotein (glycophorin A). 10066782 0 57 gly N-glycosylation 29:43 arg1 the human interleukin-6 receptor the human interleukin-6 receptor PUBTATOR interleukin-6 receptor 3570 Disulfide bond structure and N-glycosylation sites of the extracellular domain of the human interleukin-6 receptor. 19556306 6 18 gly TLR4 975:978 arg1 complex type N-glycans TLR4 complex type N-glycans PUBTATOR TLR4 7099 The amount of the 130 kDa TLR4(C88A) with complex type N-glycans expressed on the cell surface depended on that of MD-2 transfected. 19379732 3 5 gly glycosylated 512:523 arg1 WT hSVCT1 WT hSVCT1 PUBTATOR WT hSVCT1 9963 PNGase F treatment confirmed that WT hSVCT1 (approximately 70-100 kDa) is glycosylated and site-directed mutagenesis of the three putative N-glycosylation sites, Asn138, Asn144, Asn230, demonstrated that mutants N138Q and N144Q were glycosylated (approximately 68-90 kDa) with only 31-65% of WT l-ascorbic acid (AA) uptake while the glycosylation profile of N230Q remained unaltered (approximately 98% of WT activity). 14981520 9 34 gly glycosylation 1494:1506 arg1 seipin seipin PUBTATOR seipin 26580 The amino acid substitutions N88S and S90L affect glycosylation of seipin and result in aggregate formation leading to neurodegeneration. 9530955 1 2 gly glycoprotein 159:170 arg1 The MUC1 glycoprotein The MUC1 glycoprotein PUBTATOR MUC1 glycoprotein 4582 The MUC1 glycoprotein, epitectin, a component of the human bladder epithelium, was purified from human urine. 18508581 4 12 gly hyperglycosylated 588:604 arg1 EpCAM EpCAM PUBTATOR EpCAM 4072 EpCAM was hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. 8942648 3 29 gly mapping 375:381 arg1 human TPO human TPO PUBTATOR TPO 7066 Peptide, disulfide, and glycosylation mapping of human TPO from residues 1 to 246 has been carried out using liquid chromatography-electrospray mass spectrometry (LC-ESMS). 17322565 0 38 gly Glycosylation 0:12 arg1 endothelial lipase endothelial lipase PUBTATOR endothelial lipase 9388 Glycosylation of endothelial lipase at asparagine-116 reduces activity and the hydrolysis of native lipoproteins in vitro and in vivo. 2049076 8 20 part_of IFN-alpha 1198:1206 arg1 Thr-106 IFN-alpha 2 Thr-106 PUBTATOR SpecificSite IFN-alpha 2 3440 Thr-106 These results suggested that Thr-106 of natural IFN-alpha 2 carries O-linked carbohydrates. 9677337 3 29 gly glycosylation 614:626 arg1 both heavy chains both heavy chains OGER chains P19827 In this study, using matrix-assisted laser desorption ionization-time-of-flight MS and amino acid sequencing of tryptic peptides, we provide a detailed analysis of the glycosylation pattern of both heavy chains. 22387313 0 42 gly N-glycosylation 0:14 arg1 the mammalian dipeptidyl aminopeptidase-like protein 10 the mammalian dipeptidyl aminopeptidase-like protein 10 PUBTATOR dipeptidyl aminopeptidase-like protein 10 57628 N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. 22387313 0 42 gly N-glycosylation 0:14 arg1 DPP10 DPP10 PUBTATOR DPP10 57628 N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. 12956774 1 8 gly glycoprotein 233:244 arg1 chemokines chemokines PUBTATOR Duffy antigen/receptor for chemokines 2532 The Duffy antigen/receptor for chemokines (DARC), a seven-transmembrane glycoprotein carrying the Duffy (Fy) blood group, acts as a widely expressed promiscuous chemokine receptor. 15477100 0 64 gly Zn-alpha2-glycoprotein 46:67 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein. 14718370 2 33 part_of subdomains 233:242 arg1 MUC5B MUC5B subdomains PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 33 part_of subdomains 233:242 arg1 MUC5AC MUC5AC subdomains PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 75 part_of Cys1 201:204 arg1 MUC5B MUC5B Cys1 PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 75 part_of Cys1 201:204 arg1 MUC5AC MUC5AC Cys1 PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 80 part_of Cys5 210:213 arg1 MUC5B MUC5B Cys5 PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 80 part_of Cys5 210:213 arg1 MUC5AC MUC5AC Cys5 PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 84 part_of Cys1 219:222 arg1 MUC5B MUC5B Cys1 PUBTATOR AminoAcid MUC5B Q9HC84 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 14718370 2 84 part_of Cys1 219:222 arg1 MUC5AC MUC5AC Cys1 PUBTATOR AminoAcid MUC5AC P98088 Cys1 and Cys5 and Cys1 and Cys3 subdomains The entire Cys1 and Cys5 and Cys1 and Cys3 subdomains in MUC5AC and MUC5B, respectively, each with six carboxyl terminal histidine residues, were pulse-labeled with [(35)S]cysteine/methionine, and the labeled proteins were examined in the culture medium. 19671700 0 94 gly repeats 57:63 arg1 ADAMTS-like 1/punctin-1 ADAMTS-like 1 repeats PUBTATOR ADAMTS-like 1 100757121 Post-translational modification of thrombospondin type-1 repeats in ADAMTS-like 1/punctin-1 by C-mannosylation of tryptophan. 11372680 6 34 part_of contains 818:825 arg1 mouse ZPI AND P1-P1' mouse ZPI P1-P1' PUBTATOR SiteSequence ZPI 217847 P1-P1 Like human ZPI, mouse ZPI contains tyrosine-serine (P1-P1') at its reactive center in contrast to the rat molecule which contains tyrosine-cysteine. 1371281 1 15 gly glycosylation 91:103 arg1 CK CK OGER CK) 8 P05787 The glycosylation of human cytokeratin (CK) 8 and 18 was studied after metabolic labeling of HT29 colonic cells with [3H]glucosamine. 15498570 5 31 gly N-glycosylation 1068:1082 arg1 hPAP21 hPAP21 PUBTATOR hPAP21 84279 Interestingly, the extracellular forms were primarily sensitive to PNG F, not Endo H, implying that complex N-glycosylation could be required for the secretion of hPAP21. 9295302 2 15 gly phosphoglycoprotein 205:223 arg1 Multidrug resistance protein Multidrug resistance protein PUBTATOR Multidrug resistance protein 4363 Multidrug resistance protein, MRP, is a 190-kDa integral membrane phosphoglycoprotein that belongs to the ATP-binding cassette superfamily of transport proteins and is capable of conferring resistance to multiple chemotherapeutic agents. 8390218 4 96 gly AAT 1354:1356 arg1 the carbohydrate chains AAT the carbohydrate chains PUBTATOR AAT 5265 These results indicate that a characteristic feature of the carbohydrate chains of AAT from patients with HCC is an increment in fucosylation. 11390601 3 42 part_of gp120 845:849 arg1 N301 gp120 N301 PUBTATOR SpecificSite gp120 3700 N301 Of the glycosylation sites that were evaluated, those proximal to the V1/V2 loops (N135, N141, N156, N160) and the V3 loops (N301) of gp120 were functionally critical. 1517205 1 13 part_of protein 341:347 arg1 Ser-53 protein Z Ser-53 Cterm SpecificSite protein Z Ser-53 We have recently discovered unusual sugar chains (xylose (Xyl)-glucose (Glc) and (Xyl)2-Glc) linked to a serine residue in the epidermal growth factor (EGF)-like domains of human and bovine clotting factors VII (Ser-52), IX (Ser-53), and protein Z (Ser-53), in addition to bovine platelet glycoprotein thrombospondin. 17711303 12 66 gly N-glycosylation 1808:1822 arg1 the hKOR the hKOR PUBTATOR hKOR 4986 Thus, N-glycosylation of the hKOR plays important roles in stability and trafficking along the biosynthesis pathway of the receptor protein as well as agonist-induced receptor regulation. 3935432 6 19 gly O-linked 867:874 arg1 the IL-2 protein AND Carbohydrates the IL-2 protein Carbohydrates PUBTATOR IL-2 protein 3558 Carbohydrates are O-linked to the IL-2 protein via threonine-3 of the polypeptide chain. 23269669 4 19 gly glycosylated 685:696 arg1 GC-C GC-C PUBTATOR GC-C 2984 GC-C is glycosylated in the extracellular domain, and differentially glycosylated forms that are resident in the endoplasmic reticulum (130 kDa) and the plasma membrane (145 kDa) bind the ST peptide with equal affinity. 7642555 9 10 gly c-Myc 1120:1124 arg1 the major O-GlcNAc glycosylation site c-Myc the major O-GlcNAc glycosylation site PUBTATOR c-Myc 4609 These analyses show that threonine 58, an in vivo phosphorylation site in the transactivation domain, is the major O-GlcNAc glycosylation site of c-Myc. 7642555 9 63 gly glycosylation 1098:1110 arg1 c-Myc c-Myc PUBTATOR c-Myc 4609 These analyses show that threonine 58, an in vivo phosphorylation site in the transactivation domain, is the major O-GlcNAc glycosylation site of c-Myc. 8631363 9 62 gly 0-glycosylation 1551:1565 arg1 tumor necrosis factor-alpha tumor necrosis factor-alpha PUBTATOR tumor necrosis factor-alpha 7124 An amino acid sequence analysis of the glycosylated peptides was performed after Staphylococcus aureus V8 protease digestion of tumor necrosis factor-alpha had been completed, and it was proved that the 0-glycosylation site of tumor necrosis factor-alpha was Ser 4. 21733844 7 45 gly glycosylated 988:999 arg1 glycosylated hAQP10 glycosylated hAQP10 PUBTATOR hAQP10 89872 In contrast, glycosylated hAQP10 showed increased thermostability of 3-6 °C compared with the nonglycosylated protein, suggesting a stabilizing effect of the N-linked glycan. 18490449 0 100 gly sialylglycoprotein 24:41 arg1 Opalin Opalin PUBTATOR Opalin 226115 Opalin, a transmembrane sialylglycoprotein located in the central nervous system myelin paranodal loop membrane. 8687384 2 55 gly glycans 552:558 arg1 Bowes t-PA t-PA glycans PUBTATOR t-PA P00750 This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues. 8687384 2 25 gly carry 579:583 arg1 Bowes t-PA AND sialic acid Bowes t-PA sialic acid PUBTATOR t-PA P00750 This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues. 25092234 0 34 gly N-glycosylation 14:28 arg1 human factor XI human factor XI OGER factor XI P03951 Site-specific N-glycosylation analysis of human factor XI: Identification of a noncanonical NXC glycosite. 18787108 6 69 gly glycoprotein 856:867 arg1 CTRP9 CTRP9 PUBTATOR CTRP9 239126 CTRP9 is a secreted glycoprotein with multiple post-translational modifications in its collagen domain that include hydroxylated prolines and hydroxylated and glycosylated lysines. 8243461 8 17 gly residues 1695:1702 arg1 factor X factor X residues OGER factor X P00742 It appears that carbohydrate residues in factor X play an important role in the activation of the zymogen. 10921916 7 31 gly carbohydrate 1035:1046 arg1 CD45 CD45 carbohydrate PUBTATOR CD45 5788 The interaction between CD45 and GII is dependent on the active site of GII, is mediated through the carbohydrate on CD45, and can be inhibited with mannose. 20406422 2 76 gly N-glycosylation 428:442 arg1 CLN7 CLN7 PUBTATOR CLN7 256471 In this study fluorescence protease protection assays and mutational analyses revealed the N- and C-terminal tails of CLN7 in the cytosol and two N-glycosylation sites at N371 and N376. 2813359 0 69 gly erythropoietin 88:101 arg1 sugar chain structure erythropoietin sugar chain structure PUBTATOR erythropoietin 2056 Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells. 9450956 10 53 gly C-glycosylation 1286:1300 arg1 recombinant human interleukin 12 recombinant human interleukin 12 OGER interleukin 12 Two of these proteins were analyzed protein chemically, which showed partial C-glycosylation of recombinant human interleukin 12. 14699159 4 74 gly glycosylation 968:980 arg1 newly synthesized p90ATF6 newly synthesized p90ATF6 Cterm p90ATF6 22926 Here we show that ER Ca(2+) depletion stress, a triggering mechanism for the UPR, induces the formation of ATF6(f), which represents de novo partial glycosylation of newly synthesized p90ATF6. 19413349 2 80 part_of contains 268:275 arg1 Rat melanopsin AND Asn31 Rat melanopsin Asn31 and Asn35 PUBTATOR AminoAcid Rat melanopsin 192223 Asn31 and Asn35 Rat melanopsin contains two potential sites (Asn31 and Asn35) for N-linked glycosylation in the N-terminal extracellular part. 1445902 0 41 gly glycosylation 21:33 arg1 baculovirus-expressed mouse interleukin-3 baculovirus-expressed mouse interleukin-3 PUBTATOR interleukin-3 16187 Determination of the glycosylation patterns, disulfide linkages, and protein heterogeneities of baculovirus-expressed mouse interleukin-3 by mass spectrometry. 1533633 8 61 gly unglycosylated 1173:1186 arg1 The unglycosylated alpha-subunit The unglycosylated alpha-subunit OGER subunit P06865 The unglycosylated alpha-subunit, resulting from genetic alteration of all three glycosylation sites or synthesis of the wild-type protein in the presence of tunicamycin, was catalytically inactive. 22902367 4 11 gly glycosylated 676:687 arg1 Necl-5 Necl-5 OGER Necl-5 P15151 To understand the molecular basis of their adhesion and specificity, we determined the crystal structures of natively glycosylated full ectodomains or adhesive fragments of all four nectins and Necl-5. 22902367 4 11 gly glycosylated 676:687 arg1 all four nectins all four nectins PUBTATOR nectins 5818 To understand the molecular basis of their adhesion and specificity, we determined the crystal structures of natively glycosylated full ectodomains or adhesive fragments of all four nectins and Necl-5. 19855092 7 18 gly desialylated 1146:1157 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin 11450 Activity of desialylated adiponectin was comparable to control adiponectin in L6 myotubes and acute assays in adiponectin(-/-) mice. 2243102 3 56 gly polylactosaminoglycans 306:327 arg1 lamp-2 lamp-2 polylactosaminoglycans PUBTATOR lamp-2 3920 We have localized the polylactosaminoglycans to specific sites on lamp-1 and lamp-2 purified from human chronic myelogenous leukemia cells. 2243102 3 56 gly polylactosaminoglycans 306:327 arg1 lamp-1 lamp-1 polylactosaminoglycans PUBTATOR lamp-1 3916 We have localized the polylactosaminoglycans to specific sites on lamp-1 and lamp-2 purified from human chronic myelogenous leukemia cells. 2209609 1 16 gly glycoprotein 251:262 arg1 sCD4 sCD4 PUBTATOR sCD4 79966 Structures of the N-linked oligosaccharides of a recombinant soluble form of human CD4 glycoprotein (sCD4) have been investigated by enzymic microsequencing. 2209609 1 16 gly glycoprotein 251:262 arg1 human CD4 glycoprotein human CD4 glycoprotein PUBTATOR CD4 glycoprotein P01730 Structures of the N-linked oligosaccharides of a recombinant soluble form of human CD4 glycoprotein (sCD4) have been investigated by enzymic microsequencing. 16332679 0 95 gly o-glycosylation 71:85 arg1 sp1 sp1 OGER sp1 P08047 Insulin dynamically regulates calmodulin gene expression by sequential o-glycosylation and phosphorylation of sp1 and its subcellular compartmentalization in liver cells. 2049076 0 66 gly O-glycosylated 36:49 arg1 Natural human interferon-alpha 2 Natural human interferon-alpha 2 PUBTATOR interferon-alpha 2 3440 Natural human interferon-alpha 2 is O-glycosylated. 16342937 3 7 gly glycoproteins 255:267 arg1 ZP2 ZP2 PUBTATOR ZP2 22787 Mouse zonae are composed of three glycoproteins (ZP1, ZP2, and ZP3), while rat zonae contain four (ZP1, ZP2, ZP3, and ZP4/ZPB). 16342937 3 7 gly glycoproteins 255:267 arg1 ZP3 ZP3 PUBTATOR ZP3 22788 Mouse zonae are composed of three glycoproteins (ZP1, ZP2, and ZP3), while rat zonae contain four (ZP1, ZP2, ZP3, and ZP4/ZPB). 16342937 3 7 gly glycoproteins 255:267 arg1 ZP1 ZP1 PUBTATOR ZP1 22786 Mouse zonae are composed of three glycoproteins (ZP1, ZP2, and ZP3), while rat zonae contain four (ZP1, ZP2, ZP3, and ZP4/ZPB). 7663166 6 55 gly N-glycosylated 977:990 arg1 rmOSF-2 rmOSF-2 Cterm rmOSF-2 50706 N-Glycanase digestion resulted in the same mobility shift of OSF-2, indicating that rmOSF-2 expressed in insect cells is N-glycosylated. 7574684 1 20 gly glycoprotein 241:252 arg1 a human neurotrophin receptor trkB a human neurotrophin receptor trkB PUBTATOR trkB 4915 An extracellular domain of a human neurotrophin receptor trkB was expressed in Chinese hamster ovary cells and isolated as a glycoprotein possessing binding activity for brain-derived neurotrophic factor. 8243461 4 5 gly had 725:727 arg1 bovine factor X AND an O-linked oligosaccharide chain bovine factor X an O-linked oligosaccharide chain OGER factor X P00742 By combined analysis of amino acid sequence and sialic acid content, we found that bovine factor X had an O-linked oligosaccharide chain linked to Thr26, and human factor X had four carbohydrate-attachment sites, namely, O-glycosidic linkages to Thr17 and Thr29, respectively, and N-glycosidic linkages to Asn39 and Asn49, respectively, in their activation peptides. 8243461 4 84 gly had 799:801 arg1 human factor X AND four carbohydrate-attachment sites human factor X four carbohydrate-attachment sites OGER factor X P00742 By combined analysis of amino acid sequence and sialic acid content, we found that bovine factor X had an O-linked oligosaccharide chain linked to Thr26, and human factor X had four carbohydrate-attachment sites, namely, O-glycosidic linkages to Thr17 and Thr29, respectively, and N-glycosidic linkages to Asn39 and Asn49, respectively, in their activation peptides. 12063277 4 27 gly glycosylation 590:602 arg1 HERG channels HERG channels PUBTATOR HERG channels 3757 In this study, we used the approaches of site-directed mutagenesis and biochemical modification to inhibit N-linked glycosylation and studied the role of glycosylation in the cell surface expression and turnover of HERG channels. 6177036 1 9 gly glycoproteins 110:122 arg1 mouse brain Thy-1 glycoproteins mouse brain Thy-1 glycoproteins PUBTATOR Thy-1 glycoproteins 21838 The amino acid sequences of mouse brain Thy-1 glycoproteins are shown to be homologous to those of variable-region immunoglobulin domains. 1577715 2 74 gly factor 213:218 arg1 The asparagine-linked oligosaccharide chains von Willebrand factor The asparagine-linked oligosaccharide chains PUBTATOR von Willebrand factor 7450 The asparagine-linked oligosaccharide chains of human von Willebrand factor (vWF) purified from pooled plasma were quantitatively liberated from the polypeptide moiety by hydrazinolysis. 12944413 3 8 gly SERT 591:594 arg1 sialic acid-defective Lec4 Chinese hamster ovary (CHO) cells SERT sialic acid-defective Lec4 Chinese hamster ovary (CHO) cells PUBTATOR SERT 6532 In this study, we investigated the contribution of N-glycosyl modification to the structure and function of SERT in two model systems: wild-type SERT expressed in sialic acid-defective Lec4 Chinese hamster ovary (CHO) cells and a mutant form (after site-directed mutagenesis of Asn-208 and Asn-217 to Gln) of SERT, QQ, expressed in parental CHO cells. 19276170 3 54 gly COMP 612:615 arg1 the type 3 repeats COMP the type 3 repeats OGER COMP P49747 In this study, we determined the structure of a recombinant protein that contains the last epidermal growth factor repeat, the type 3 repeats and the C-terminal domain (CTD) of COMP to 3.15-A resolution limit by X-ray crystallography. 8172892 4 82 part_of AP 645:646 arg1 residues 157-165 AP alpha residues 157-165 PUBTATOR SpecificSite AP alpha 2028 residues 157-165 A nonapeptide (AP alpha-D4, residues 157-165) and an undecapeptide (AP alpha-D5, 166-176) derived from AP alpha contained Thr-159 and Thr-169, neither of which could be identified using a gas-phase protein sequencer. 8172892 4 71 part_of contained 742:750 arg1 AP alpha-D5, 166-176 AND Thr-159 AP alpha Thr-159 and Thr-169 PUBTATOR SpecificSite AP alpha 2028 Thr-159 and Thr-169 A nonapeptide (AP alpha-D4, residues 157-165) and an undecapeptide (AP alpha-D5, 166-176) derived from AP alpha contained Thr-159 and Thr-169, neither of which could be identified using a gas-phase protein sequencer. 22448645 3 13 gly N-glycosylated 547:560 arg1 an N-glycosylated form an N-glycosylated form OGER N-glycosylated form of the 5-HT(7 P34969 Western blot analysis of HEK293T cells transiently expressing the 5-HT(7(a)) receptor in the presence of tunicamycin gave rise to a band shift, indicating the existence of an N-glycosylated form of the 5-HT(7(a)) receptor. 18952059 4 21 gly glycosylation 461:473 arg1 IZUMO IZUMO PUBTATOR IZUMO 73456 The expression of N204Q-IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO. 7918467 7 32 part_of apoA-II 810:816 arg1 Cys6 apoA-II Cys6 PUBTATOR AminoAcid apoA-II 336 Cys6 Using a combination of manual Edman degradations and mass spectrometric analysis on a purified cluster of chymotryptic fragments, we identified an intramolecular disulfide bridge between Cys8 and Cys114 and an intermolecular bridge between Cys116 of apoD and Cys6 of apoA-II. 7918467 7 21 part_of apoD 793:796 arg1 Cys114 apoD Cys8 and Cys114 PUBTATOR AminoAcid apoD 347 Cys8 and Cys114 Using a combination of manual Edman degradations and mass spectrometric analysis on a purified cluster of chymotryptic fragments, we identified an intramolecular disulfide bridge between Cys8 and Cys114 and an intermolecular bridge between Cys116 of apoD and Cys6 of apoA-II. 3667593 4 50 gly IFN-beta 714:721 arg1 The major oligosaccharide IFN-beta The major oligosaccharide PUBTATOR IFN-beta 3456 The major oligosaccharide of the recombinant IFN-beta is remarkably homogeneous with respect to terminal galactose sialylation. 12063277 7 23 gly nonglycosylated 1058:1072 arg1 nonglycosylated HERG channel nonglycosylated HERG channel PUBTATOR HERG channel 3757 Pulse-chase experiments show that the turnover rate of nonglycosylated HERG channel is faster than that of the glycosylated form, suggesting that N-linked glycosylation plays an important role in HERG channel stability. 17395589 3 50 gly contains 259:266 arg1 ADAMTS13 AND eight thrombospondin type 1 repeats ADAMTS13 eight thrombospondin type 1 repeats PUBTATOR ADAMTS13 11093 ADAMTS13 contains eight thrombospondin type 1 repeats (TSR), seven of which contain a consensus sequence for the direct addition of fucose to the hydroxyl group of serine or threonine. 10200178 2 102 gly PrP 398:400 arg1 the N-linked glycans PrP the N-linked glycans OGER PrP Q9NP58 A partial site-specific study of the N-linked glycans from hamster PrP has previously been carried out by mass spectrometry [Stahl, N., Baldwin, M. A., Teplow, D. B., Hood, L., Gibson, B. W., Burlingame, A. L., and Prusiner, S. B. (1993) Biochemistry 32, 1991-2002] and revealed that the glycosylation at Asn-181 (equivalent to mouse 180) is heterogeneous, comprising over 30 glycoforms. 18642129 4 93 gly FVII 604:607 arg1 N- FVII N- OGER FVII P08709 Despite glycosylation of recombinant FVIIa has been fully characterized, nothing is reported on the N- and O-glycans of plasma-derived FVII (pd-FVII) and on their structural heterogeneity at each glycosylation site. 17222411 1 36 gly sialoglycoprotein 140:156 arg1 Podoplanin Podoplanin OGER Podoplanin Q86YL7 Podoplanin (Aggrus) is a mucin-type sialoglycoprotein that plays a key role in tumor cell-induced platelet aggregation. 7505568 1 0 gly glycoprotein 102:113 arg1 MAG MAG PUBTATOR MAG 4099 Myelin-associated glycoprotein (MAG) is a neural cell adhesion molecule expressing the L2/HNK-1 carbohydrate epitope. 7505568 1 0 gly glycoprotein 102:113 arg1 Myelin-associated glycoprotein Myelin-associated glycoprotein PUBTATOR Myelin-associated glycoprotein 4099 Myelin-associated glycoprotein (MAG) is a neural cell adhesion molecule expressing the L2/HNK-1 carbohydrate epitope. 14749323 11 82 gly glycosylation 2054:2066 arg1 OAT1 OAT1 PUBTATOR OAT1 9356 This study is the first molecular identification and characterization of glycosylation of OAT1 and may provide important insights into the structure-function relationships of the organic anion transporter family. 11733580 2 6 gly O-glycosylated 375:388 arg1 CCR5 CCR5 PUBTATOR CCR5 1234 We provide evidence that CCR5 is O-glycosylated on serine 6 in the NH2 terminus. 16877748 5 102 gly N-glycosylation 815:829 arg1 mouse sICAM-1 mouse sICAM-1 Cterm sICAM-1 3383 The present study was aimed at elucidating the N-glycosylation of mouse sICAM-1 expressed in wild-type CHO cells with regard to sialylation, N-glycan profile, and N-glycosylation sites. 10814696 1 66 gly A 393:393 arg1 the carbohydrate component arylsulfatase A the carbohydrate component PUBTATOR arylsulfatase A 410 It has been shown that the concentration of arylsulfatase A increases in the body fluids of patients with some forms of cancer, and the carbohydrate component of arylsulfatase A synthesized in tumor tissues and transformed cells undergoes increased sialylation, phosphorylation and sulfation. 1991473 8 59 part_of hLH 1255:1257 arg1 Asn78 hLH alpha Asn78 PUBTATOR AminoAcid hLH alpha 1081 Asn78 The oligosaccharides at Asn78 (hLH alpha) are sialylated rather than sulphated and contain the unique sequence NeuAc alpha 2-6 GalNAc beta 1-4GlcNAc beta 1-2 Man alpha 1-3 as part of the majority of mono- and disialylated compounds. 24927598 4 36 gly deglycosylated 580:593 arg1 ZIP14 ZIP14 PUBTATOR ZIP14 23516 In an attempt to dissect the molecular mechanisms by which iron regulates ZIP14 levels, we found that ZIP14 is endocytosed, extracted from membranes, deglycosylated, and degraded by proteasomes. 23376777 3 88 gly glycosylation 393:405 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 Site-directed mutagenesis was performed on the four putative extracellular N-linked glycosylation sites of KCC4 to determine the role of these sites in KCC4 half-life, cell surface expression, and transporter activity, as well as in KCC4-dependent tumor formation. 15477100 1 67 gly Zn-alpha2-glycoprotein 70:91 arg1 ZAG ZAG PUBTATOR ZAG 563 Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. 15477100 1 67 gly Zn-alpha2-glycoprotein 70:91 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Zn-alpha2-glycoprotein (ZAG) is a 41 kDa soluble protein that is present in most bodily fluids. 479158 0 43 gly variants 38:45 arg1 Carbohydrate plasminogen variants Carbohydrate OGER plasminogen variants P00747 Carbohydrate of the human plasminogen variants. 10756055 0 45 gly glycosylation 9:21 arg1 CXCR4 CXCR4 PUBTATOR CXCR4 7852 N-linked glycosylation of CXCR4 masks coreceptor function for CCR5-dependent human immunodeficiency virus type 1 isolates. 10861210 5 15 part_of AE1 819:821 arg1 N555 AE1 N555 PUBTATOR SpecificSite AE1 6521 N555 Moving the N-glycosylation site to the preceding extracellular loop in an AE1 glycosylation mutant (N555) resulted in processing of the oligosaccharide and production of a complex form of AE1. 2415652 6 99 gly beta 1248:1251 arg1 The O-linked oligosaccharides hCG beta The O-linked oligosaccharides PUBTATOR hCG beta 1082 The O-linked oligosaccharides of standard hCG beta are heterogeneous in size; 13% are of the largest (hexasaccharide) form. 10995228 5 25 gly moieties 727:734 arg1 mER-beta mER moieties PUBTATOR mER 13983 Structural characterization of the carbohydrate moieties on mER-beta, overexpressed in insect Sf9 cells, confirmed the presence of O-GlcNAc. 18676855 7 15 gly glycoprotein 1275:1286 arg1 NGEP NGEP PUBTATOR NGEP Q6IWH7 NGEP is a glycoprotein with predicted glycosylation sites at N809 and N824. 24058541 9 31 gly N-glycosylation 1293:1307 arg1 CLN5 CLN5 PUBTATOR CLN5 O75503 Our results suggest that there are functional differences in various N-glycosylation sites of CLN5 which affect folding, trafficking, and lysosomal function of CLN5. 14764083 0 50 gly glycosylation 21:33 arg1 human and bovine lactoferrin human and bovine lactoferrin PUBTATOR lactoferrin 280846 The role of N-linked glycosylation in the protection of human and bovine lactoferrin against tryptic proteolysis. 27966990 4 63 gly N-glycosylation 554:568 arg1 VEGFR-2 VEGFR-2 PUBTATOR VEGFR-2 P35968 However, despite its importance, the functional role of N-glycosylation of VEGFR-2 remains poorly understood. 8130392 6 134 gly contains 851:858 arg1 rHPC AND N-acetylgalactosamine rHPC N-acetylgalactosamine OGER rHPC P52873 In addition, we found that rHPC contains N-acetylgalactosamine (2.6 mol GalNAc/mol rHPC) in its Asn-linked oligosaccharides, while plasma HPC is devoid of GalNAc. 8130392 6 134 gly contains 851:858 arg1 rHPC AND 2.6 mol GalNAc/mol rHPC rHPC 2.6 mol GalNAc/mol rHPC OGER rHPC P52873 In addition, we found that rHPC contains N-acetylgalactosamine (2.6 mol GalNAc/mol rHPC) in its Asn-linked oligosaccharides, while plasma HPC is devoid of GalNAc. 20622017 3 72 gly released 370:377 arg2 kidney GGT AND N-glycans kidney GGT N-glycans OGER GGT The spectrum of N-glycans released from kidney GGT constituted a subset of the N-glycans identified from renal membrane glycoproteins. 26828122 7 64 gly glycosylated 1484:1495 arg1 the hTPPT the hTPPT PUBTATOR hTPPT 80736 Results of this study showed, for the first time, that the hTPPT is glycosylated and that N-linked glycosylation occurs at multiple sites with some of them being important for function. 22629392 7 19 gly Akt 1390:1392 arg1 O-GlcNAcylations Akt O-GlcNAcylations PUBTATOR Akt 207 Together, this study revealed an extensive crosstalk between O-GlcNAcylations and phosphorylations of Akt and demonstrated O-GlcNAcylation as a new regulatory modification for Akt signaling. 20837471 6 0 part_of Thr 1105:1107 arg1 ANGPTL3 ANGPTL3 Thr PUBTATOR SpecificSite ANGPTL3 Q9Y5C1 Thr(226) Screening of a panel of proteins known to affect lipid metabolism for potential sites glycosylated by GalNAc-T2 led to identification of Thr(226) adjacent to the proprotein convertase processing site in ANGPTL3. 21980294 1 61 gly glycoprotein 125:136 arg1 herpes simplex virus (HSV) glycoprotein D herpes simplex virus (HSV) glycoprotein D PUBTATOR glycoprotein D 2532 Binding of herpes simplex virus (HSV) glycoprotein D (gD) to a cell surface receptor is required to trigger membrane fusion during entry into host cells. 17960739 9 55 gly glycosylated 1248:1259 arg1 CD9P-1 CD9P-1 PUBTATOR CD9P-1 5738 Finally, 2-D PAGE and lectino-blot analyses have revealed the presence of at least 17 glycosylated isoforms of CD9P-1 at cell surface. 1694784 0 12 gly HC 91:92 arg1 the three carbohydrate prosthetic groups protein HC the three carbohydrate prosthetic groups PUBTATOR protein HC 259 Location and characterization of the three carbohydrate prosthetic groups of human protein HC. 20805301 5 69 part_of CYP2W1 711:716 arg1 Asn177 CYP2W1 Asn177 PUBTATOR AminoAcid CYP2W1 54905 Asn177 Bioinformatic analysis identified Asn177 as the only possible glycosylation site of CYP2W1, which was supported by the inability of an N177A mutant to be glycosylated in HEK 293 cells. 2015821 5 43 gly deglycosylation 980:994 arg1 the unfractionated alpha 1-antichymotrypsin the unfractionated alpha 1-antichymotrypsin PUBTATOR alpha 1-antichymotrypsin 12 The N-glycanase was used for the deglycosylation of the unfractionated alpha 1-antichymotrypsin; the successive removal of the N-linked complex-type oligosaccharide side chains of alpha 1-antichymotrypsin was studied in the presence of detergents. 2015821 5 60 gly 1-antichymotrypsin 1133:1150 arg1 the N-linked complex-type oligosaccharide side chains alpha 1-antichymotrypsin the N-linked complex-type oligosaccharide side chains PUBTATOR alpha 1-antichymotrypsin 12 The N-glycanase was used for the deglycosylation of the unfractionated alpha 1-antichymotrypsin; the successive removal of the N-linked complex-type oligosaccharide side chains of alpha 1-antichymotrypsin was studied in the presence of detergents. 19671700 7 71 gly TSR1 1247:1250 arg1 C-Mannosylation TSR1 C-Mannosylation PUBTATOR TSR1 Q2NL82 C-Mannosylation of TSR1 of the related protease ADAMTS5 was also identified. 12731887 0 35 gly glycosylation 2:14 arg1 p67 p67 PUBTATOR p67 64370 A glycosylation site, 60SGTS63, of p67 is required for its ability to regulate the phosphorylation and activity of eukaryotic initiation factor 2alpha. 18467335 9 27 gly N-glycosylated 997:1010 arg1 PCI PCI OGER PCI P05154 PCI was N-glycosylated at all three potential N-glycosylation sites, Asn-230, Asn-243, and Asn-319, but a small fraction of PCI lacked the N-glycan at Asn-243. 24927598 7 39 part_of N102 1011:1014 arg1 ZIP14 ZIP14 N102 PUBTATOR SpecificSite ZIP14 23516 N102 Asparagine-linked (N-linked) glycosylation of ZIP14, particularly the glycosylation at N102, was required for efficient membrane extraction of ZIP14 and therefore is necessary for its iron sensitivity. 19951703 10 69 gly deglycosylated 1375:1388 arg1 deglycosylated UGT1A9 proteins deglycosylated UGT1A9 proteins PUBTATOR UGT1A9 proteins 54600 The thermal stability analysis of the mutated and deglycosylated UGT1A9 proteins supported the findings. 18420026 6 20 gly glycoprotein 1539:1550 arg1 human NECL1 human NECL1 PUBTATOR NECL1 57863 Based on the results of N-Glycosidase F treatment with human fetal brain tissue and lysates from transient transfection with human wild-type or glycosylation site mutant NECL1 in 293ET cells, we demonstrated that human NECL1 is an N-linked glycoprotein with a single glycosylation site at position N290KS. 1820200 1 1 gly glycoprotein 231:242 arg1 the heterodimeric glycoprotein hormone the heterodimeric glycoprotein hormone PUBTATOR glycoprotein hormone human chorionic gonadotrophin (hCG) 93659 Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respectively. 1820200 1 18 gly N-glycosylated 378:391 arg1 hCG beta hCG beta PUBTATOR hCG beta 1082 Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respectively. 1820200 1 18 gly N-glycosylated 378:391 arg1 subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) OGER subunits hCG alpha P01215 Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respectively. 1820200 1 39 gly N-glycosylated 327:340 arg1 subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) subunits hCG alpha OGER subunits hCG alpha P01215 Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respectively. 1820200 1 42 gly N-glycosylation 188:202 arg1 hCG glycoprotein hormone human chorionic gonadotrophin (hCG) PUBTATOR glycoprotein hormone human chorionic gonadotrophin (hCG) 93659 Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respectively. 9169007 11 11 gly antithrombin 2012:2023 arg1 all carbohydrate chains antithrombin all carbohydrate chains PUBTATOR antithrombin 462 These results indicate that all carbohydrate chains of recombinant antithrombin adversely affect heparin-binding affinity to an extent that correlates with their relative proximity to the putative heparin-binding site in antithrombin. 8407981 4 34 gly glycosylated 500:511 arg1 The P-450(arom) protein The P-450(arom) protein PUBTATOR P-450(arom) protein 55010 The P-450(arom) protein expressed in the insect cells was glycosylated, and the sugar chain was sensitive to Endo H. 9169007 0 100 gly antithrombin 56:67 arg1 individual carbohydrate chains antithrombin individual carbohydrate chains PUBTATOR antithrombin 462 Effect of individual carbohydrate chains of recombinant antithrombin on heparin affinity and on the generation of glycoforms differing in heparin affinity. 19413349 9 41 gly glycosylated 1646:1657 arg1 heterologously expressed rat melanopsin heterologously expressed rat melanopsin PUBTATOR melanopsin 192223 In conclusion, we have shown that heterologously expressed rat melanopsin is both N-linked and O-linked glycosylated and that N-linked glycosylation is not crucial for the melanopsin response to light. 21757827 5 11 gly O-glycosylated 776:789 arg1 YIPF3 YIPF3 PUBTATOR YIPF3 25844 Biochemical and immunofluorescence experiments strongly indicated that YIPF3 is synthesized in the ER as a N-glycosylated form (40 kDa), is then O-glycosylated in the Golgi apparatus to become a lower mobility form (46 kDa) and finally becomes a higher mobility form cleaved at its C-terminal luminal domain (36 kDa). 21757827 5 41 gly N-glycosylated 738:751 arg1 YIPF3 YIPF3 PUBTATOR YIPF3 25844 Biochemical and immunofluorescence experiments strongly indicated that YIPF3 is synthesized in the ER as a N-glycosylated form (40 kDa), is then O-glycosylated in the Golgi apparatus to become a lower mobility form (46 kDa) and finally becomes a higher mobility form cleaved at its C-terminal luminal domain (36 kDa). 3342257 4 25 gly kappa-casein 1356:1367 arg1 Five oligosaccharide alditols kappa-casein Five oligosaccharide alditols OGER kappa-casein P07498 Five oligosaccharide alditols (III-VII) were the novel carbohydrate chains of kappa-casein from mammalian milk. 3342257 4 25 gly kappa-casein 1356:1367 arg1 the novel carbohydrate chains kappa-casein the novel carbohydrate chains OGER kappa-casein P07498 Five oligosaccharide alditols (III-VII) were the novel carbohydrate chains of kappa-casein from mammalian milk. 23661698 5 50 gly deglycosylated 651:664 arg1 deglycosylated ephrin-A1 deglycosylated ephrin-A1 PUBTATOR ephrin-A1 1942 We found that deglycosylated ephrin-A1 does not efficiently induce EphA2 receptor internalization and degradation, and does not activate the downstream signaling pathways involved in cell migration and proliferation. 3497198 5 32 part_of H-2Kb 769:773 arg1 Asn86 H-2Kb Asn86 PUBTATOR AminoAcid H-2Kb 14972 Asn86 However, Asn86 of H-2Kb contained a higher ratio (5 to 1), while Asn86 of H-2Kk a lower ratio (1.5 to 1). 3497198 5 40 part_of H-2Kk 825:829 arg1 Asn86 H-2Kk a Asn86 PUBTATOR AminoAcid H-2Kk a 14972 Asn86 However, Asn86 of H-2Kb contained a higher ratio (5 to 1), while Asn86 of H-2Kk a lower ratio (1.5 to 1). 12391027 1 35 gly TGFbeta 263:269 arg1 glycosaminoglycan binding TGFbeta glycosaminoglycan binding PUBTATOR TGFbeta 7040 Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. 12391027 1 14 gly contains 125:132 arg1 Thrombospondin-1 AND three type 1 repeats Thrombospondin-1 three type 1 repeats PUBTATOR Thrombospondin-1 7057 Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. 12391027 1 14 gly contains 125:132 arg1 TSP-1 AND three type 1 repeats TSP-1 three type 1 repeats PUBTATOR TSP-1 7057 Thrombospondin-1 (TSP-1) contains three type 1 repeats (TSRs), which mediate cell attachment, glycosaminoglycan binding, inhibition of angiogenesis, activation of TGFbeta, and inhibition of matrix metalloproteinases. 1421756 1 64 gly contains 193:200 arg1 TfR AND Ser/Thr-linked (O-linked) oligosaccharides TfR Ser/Thr-linked (O-linked) oligosaccharides PUBTATOR TfR 7037 We have previously demonstrated that the human transferrin receptor (TfR) of approximately 90 kDa contains Ser/Thr-linked (O-linked) oligosaccharides. 1421756 1 64 gly contains 193:200 arg1 the human transferrin receptor AND Ser/Thr-linked (O-linked) oligosaccharides the human transferrin receptor Ser/Thr-linked (O-linked) oligosaccharides PUBTATOR transferrin receptor 7037 We have previously demonstrated that the human transferrin receptor (TfR) of approximately 90 kDa contains Ser/Thr-linked (O-linked) oligosaccharides. 17591618 7 76 gly glycosylated 1169:1180 arg1 CFH CFH PUBTATOR CFH P08603 Quantitative analysis showed that CFH is mainly glycosylated by complex, diantennary disialylated, non-fucosylated glycans. 8323299 4 58 gly glycosylated 619:630 arg1 lamp-1 lamp-1 PUBTATOR In lamp-1 3916 In lamp-1, Thr-171, Thr-172, Ser-179, Ser-181, and Ser-183 were fully glycosylated, whereas Ser-169 was partially glycosylated. 25485983 4 16 gly glycosylation 615:627 arg1 antithrombin antithrombin PUBTATOR antithrombin 462 We evaluated the effect of the aromatic sequon in this defective glycosylation site of antithrombin, despite of being located in a loop between the helix D and the strand 2A. 7780197 1 21 gly contains 140:147 arg1 TfR AND three N-linked oligosaccharides TfR three N-linked oligosaccharides PUBTATOR TfR 7037 The human transferrin receptor (TfR) contains three N-linked oligosaccharides and glycosylation is required for the proper folding and function of the molecule. 7780197 1 21 gly contains 140:147 arg1 The human transferrin receptor AND three N-linked oligosaccharides The human transferrin receptor three N-linked oligosaccharides PUBTATOR transferrin receptor 7037 The human transferrin receptor (TfR) contains three N-linked oligosaccharides and glycosylation is required for the proper folding and function of the molecule. 23050552 8 46 gly fibrinogen 1086:1095 arg1 The previously reported N-glycan attachment sites fibrinogen The previously reported N-glycan attachment sites PUBTATOR fibrinogen 2244 The previously reported N-glycan attachment sites of human fibrinogen could be confirmed. 10209036 3 10 gly glycoprotein 482:493 arg1 SIT SIT PUBTATOR SIT 27240 SIT is a disulfide-linked homodimeric glycoprotein that is expressed in lymphocytes. 8349699 10 17 part_of PGH 1590:1592 arg1 Arg277 PGH synthase-1 Arg277 PUBTATOR AminoAcid PGH synthase-1 19224 Arg277 Assuming that the N-glycosylation sites of PGH synthase-1 are on the luminal side of the endoplasmic reticulum (ER), and that the site of tryptic cleavage of ovine PGH synthase-1 (Arg277) is on the cytoplasmic side of the ER, we propose that both the NH2 and COOH termini of PGH synthase-1 are located in the lumen of the ER and that there are two transmembrane domains located between Asn144 and Arg277 and between Arg277 and Asn410, respectively. 1731338 1 2 gly galactoglycoprotein 146:164 arg1 galactoglycoprotein galactoglycoprotein PUBTATOR galactoglycoprotein 6693 The amino acid sequence of galactoglycoprotein purified from human plasma was elucidated to 75% completeness by using chemical degradation of peptides and glycopeptides derived from digests of the protein with seven specific proteases. 28668641 2 23 gly glycosylation 395:407 arg1 human vaspin human vaspin PUBTATOR vaspin 145264 In this study, we have investigated the glycosylation of human vaspin and its effects on biochemical properties relevant to vaspin function. 25458834 8 59 gly residue 924:930 arg1 podoplanin podoplanin residue OGER podoplanin Q86YL7 Carboxyl groups from the sialic acid residue on podoplanin and from the C terminus of the rhodocytin α subunit interact differently at this "second" binding site on CLEC-2. 9449027 7 171 gly contained 1698:1706 arg1 The beta-subunit AND fucosylated and nonfucosylated biantennary N-linked structures The beta-subunit fucosylated and nonfucosylated biantennary N-linked structures OGER subunit P0DN86 The beta-subunit from normal pregnancy hCG contained fucosylated and nonfucosylated biantennary N-linked structures; however, mono- and triantennary oligosaccharides were also identified (4.6 and 13.7%). 1388166 1 88 gly contains 207:214 arg1 TFPI AND sulfated Asn-linked oligosaccharides TFPI sulfated Asn-linked oligosaccharides PUBTATOR TFPI 7035 Tissue factor pathway inhibitor (TFPI) produced by endothelial cells contains sulfated Asn-linked oligosaccharides. 1388166 1 88 gly contains 207:214 arg1 Tissue factor pathway inhibitor AND sulfated Asn-linked oligosaccharides Tissue factor pathway inhibitor sulfated Asn-linked oligosaccharides PUBTATOR Tissue factor pathway inhibitor 7035 Tissue factor pathway inhibitor (TFPI) produced by endothelial cells contains sulfated Asn-linked oligosaccharides. 16601115 5 28 gly glycosylation 1014:1026 arg1 cystatin F cystatin F PUBTATOR cystatin F 8530 Strikingly, core sugars for one of the two N-linked glycosylation sites of cystatin F are well ordered, and their conformation and interactions with the protein indicate that this unique feature of cystatin F may modulate its inhibitory properties, in particular its reduced affinity toward asparaginyl endopeptidase compared with other cystatins. 7592613 3 75 gly glycosylated 558:569 arg1 glycodelin glycodelin PUBTATOR glycodelin 5047 Using strategies based upon fast atom bombardment and electrospray mass spectrometry we have established that glycodelin is glycosylated at Asn-28 and Asn-63. 21237175 1 4 gly glycoprotein 161:172 arg1 The translocating chain-associating membrane protein The translocating chain-associating membrane protein PUBTATOR translocating chain-associating membrane protein 23471 The translocating chain-associating membrane protein (TRAM) is a glycoprotein involved in the translocation of secreted proteins into the endoplasmic reticulum (ER) lumen and in the insertion of integral membrane proteins into the lipid bilayer. 12878160 7 10 gly VIPL 890:893 arg1 The single N-linked glycan VIPL The single N-linked glycan PUBTATOR VIPL 81562 The single N-linked glycan of VIPL remained endoglycosidase H-sensitive during a 2-h pulse-chase, even when the protein was overexpressed or mutated to allow export to the plasma membrane. 15807535 2 81 gly glycosylated 383:394 arg1 Both transiently and stably expressed ABCG2 Both transiently and stably expressed ABCG2 PUBTATOR ABCG2 9429 Both transiently and stably expressed ABCG2 are glycosylated, and treatment with peptide N-glycosidase F reduces the apparent molecular mass on SDS-PAGE gels to approximately 60 kDa. 14764083 3 29 part_of hLF 485:487 arg1 Ile130-->Thr hLF Ile130-->Thr PUBTATOR AminoAcid hLF 3131 Ile130 The analysis revealed that recombinant hLF (rhLF) with mutations Ile130-->Thr and Gly404-->Cys was about twofold more susceptible than glycosylated and unglycosylated variants with the naturally occurring Ile130 and Gly404. 14749323 6 4 part_of site 931:934 arg1 mOAT1 mOAT1 site PUBTATOR SpecificSite mOAT1 18399 site Asp-39 We showed that the putative glycosylation site Asp-39 in mOAT1 was not glycosylated but the corresponding site (Asp-39) in hOAT1 was glycosylated. 3667593 5 17 gly galactose 833:841 arg1 the human IFN-beta IFN-beta galactose PUBTATOR IFN-beta 3456 NeuAc, which is alpha 2-3-linked to galactose in the human IFN-beta secreted by Chinese hamster ovary cells, can be re-incorporated with an alpha 2-6 linkage in vitro, into enzymatically desialylated IFN-beta using rat liver Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase. 3667593 5 54 gly desialylated 984:995 arg1 enzymatically desialylated IFN-beta enzymatically desialylated IFN-beta PUBTATOR IFN-beta 3456 NeuAc, which is alpha 2-3-linked to galactose in the human IFN-beta secreted by Chinese hamster ovary cells, can be re-incorporated with an alpha 2-6 linkage in vitro, into enzymatically desialylated IFN-beta using rat liver Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase. 1421757 0 32 gly glycosylation 31:43 arg1 the human transferrin receptor the human transferrin receptor PUBTATOR transferrin receptor 7037 Identification of the O-linked glycosylation site of the human transferrin receptor. 7613477 5 101 gly glycosylation 1098:1110 arg1 LCAT LCAT PUBTATOR LCAT 3931 All four potential N-linked glycosylation sites (Asn20, Asn84, Asn272, and Asn384) of LCAT were determined to contain sialylated triantennary and/or biantennary complex structures. 7613477 5 46 gly contain 1180:1186 arg1 LCAT AND triantennary LCAT Asn84 triantennary PUBTATOR LCAT 3931 Asn20, Asn84, Asn272, and Asn384 All four potential N-linked glycosylation sites (Asn20, Asn84, Asn272, and Asn384) of LCAT were determined to contain sialylated triantennary and/or biantennary complex structures. 7613477 5 46 gly contain 1180:1186 arg1 LCAT AND sialylated triantennary and/or biantennary complex structures LCAT Asn84 sialylated triantennary and/or biantennary complex structures PUBTATOR LCAT 3931 Asn20, Asn84, Asn272, and Asn384 All four potential N-linked glycosylation sites (Asn20, Asn84, Asn272, and Asn384) of LCAT were determined to contain sialylated triantennary and/or biantennary complex structures. 10929010 1 1 gly transferrin 154:164 arg1 The N-glycans transferrin The N-glycans PUBTATOR transferrin 7018 The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed to examine N-linked oligosaccharide processing in insect cells. 8617200 5 66 part_of beta1-LAP 992:1000 arg1 Cys33 LAP Cys33 PUBTATOR AminoAcid LAP P11117 Cys33 The cysteine required for the association between LTBP-1 and beta1-LAP was mapped to Cys33 of beta1-LAP. 20511397 4 10 gly sialylation 768:778 arg1 cellular and secreted apoE cellular and secreted apoE PUBTATOR apoE 348 We investigated glycosylation and sialylation of cellular and secreted apoE from primary human macrophages by one- and two-dimensional gel electrophoresis and mass spectrometry. 20511397 4 47 gly glycosylation 750:762 arg1 cellular and secreted apoE cellular and secreted apoE PUBTATOR apoE 348 We investigated glycosylation and sialylation of cellular and secreted apoE from primary human macrophages by one- and two-dimensional gel electrophoresis and mass spectrometry. 20511397 4 72 gly apoE 805:808 arg1 sialylation apoE sialylation PUBTATOR apoE 348 We investigated glycosylation and sialylation of cellular and secreted apoE from primary human macrophages by one- and two-dimensional gel electrophoresis and mass spectrometry. 17956937 1 105 gly antigen 225:231 arg1 The oligosaccharide structures prostate specific antigen The oligosaccharide structures OGER prostate specific antigen P07288 The oligosaccharide structures of prostate specific antigen (PSA) are expected to be useful in discriminating prostate cancer from benign conditions both accompanied by increased serum PSA levels. 8631363 9 10 part_of factor-alpha 1590:1601 arg1 Ser 4 tumor necrosis factor-alpha Ser 4 PUBTATOR SpecificSite tumor necrosis factor-alpha 7124 Ser 4 An amino acid sequence analysis of the glycosylated peptides was performed after Staphylococcus aureus V8 protease digestion of tumor necrosis factor-alpha had been completed, and it was proved that the 0-glycosylation site of tumor necrosis factor-alpha was Ser 4. 24058541 0 28 gly N-glycosylation 12:26 arg1 lysosomal protein CLN5 lysosomal protein CLN5 OGER protein CLN5 O75503 The role of N-glycosylation in folding, trafficking, and functionality of lysosomal protein CLN5. 23001782 5 6 gly N-glycosylated 880:893 arg1 serum-derived hSHBG serum-derived hSHBG PUBTATOR hSHBG 6462 It was found that serum-derived hSHBG is N-glycosylated at Asn(351) and Asn(367) with average molar occupancies of 85.1 and 95.3%, respectively. 18775496 2 59 gly O-fucosylated 307:319 arg1 agrin agrin PUBTATOR agrin O00468 Here we demonstrate that agrin is O-fucosylated in a Pofut1-dependent manner, and that this glycosylation can regulate agrin function. 12590919 2 27 gly glycosylation 290:302 arg1 hENT2 hENT2 PUBTATOR hENT2 3177 It is not known whether glycosylation affects the functions of hENT2 or where hENT2 is glycosylated. 12590919 2 29 gly glycosylated 353:364 arg1 hENT2 hENT2 PUBTATOR hENT2 3177 It is not known whether glycosylation affects the functions of hENT2 or where hENT2 is glycosylated. 15044018 3 11 gly glycoprotein 589:600 arg1 IGRP IGRP PUBTATOR IGRP 57818 We show that IGRP is a glycoprotein, held in the endoplasmic reticulum by nine transmembrane domains, which is degraded in cells predominantly through the proteasome pathway that generates the major histocompatibility complex class I-presented peptides. 21669976 4 28 gly O-glycosylated 548:561 arg1 KCNE1 KCNE1 PUBTATOR KCNE1 3753 Our results show that KCNE1 is exclusively O-glycosylated at Thr-7, which is also required for N-glycosylation at Asn-5. 11710528 0 7 gly receptor 125:132 arg1 high mannose-type Asn-linked oligosaccharides transferrin receptor high mannose-type Asn-linked oligosaccharides PUBTATOR transferrin receptor 7037 Differential and cell-type specific microheterogeneity of high mannose-type Asn-linked oligosaccharides of human transferrin receptor. 19196183 8 44 gly N-glycosylated 1206:1219 arg1 bovine serum albumin bovine serum albumin OGER albumin P02768 Furthermore, unavoidable contaminants such as actin and bovine serum albumin which are not N-glycosylated could be easily depleted by using this glycoproteomic strategy. 9054441 2 49 gly contains 190:197 arg1 Plasminogen 1 AND an N-linked oligosaccharide Plasminogen 1 an N-linked oligosaccharide OGER Plasminogen 1 P00747 Plasminogen 1 contains an N-linked oligosaccharide at Asn-289 and an O-linked oligosaccharide at Thr-345. 208636 0 57 gly C-III 69:73 arg1 the oligosaccharide side chain apolipoprotein C-III the oligosaccharide side chain PUBTATOR apolipoprotein C-III 345 Characterization of the oligosaccharide side chain of apolipoprotein C-III from human plasma very low density lipoproteins. 26563299 7 30 part_of IL-15 1085:1089 arg1 Asn112 IL-15 Asn71 and Asn112 PUBTATOR AminoAcid IL-15 3600 Asn71 and Asn112 The two potential IL-15 N-glycosylation sites (Asn71 and Asn112) located at the IL-2 receptor interface were unoccupied. 17956937 2 98 gly glycoprotein 428:439 arg1 alpha(1)-antichymotrypsin alpha(1)-antichymotrypsin PUBTATOR alpha(1)-antichymotrypsin 12 A large proportion of PSA forms a covalent complex with a glycoprotein, alpha(1)-antichymotrypsin, in human blood. 24308486 0 39 gly N-glycosylation 14:28 arg1 IgE IgE PUBTATOR IgE P01854 Site-specific N-glycosylation analysis of human immunoglobulin e. Immunoglobulin E (IgE) is a heterodimeric glycoprotein involved in antiparasitic and allergic immune reactions. 24308486 0 39 gly N-glycosylation 14:28 arg1 human immunoglobulin e. Immunoglobulin E human immunoglobulin e. Immunoglobulin E PUBTATOR Immunoglobulin E P01854 Site-specific N-glycosylation analysis of human immunoglobulin e. Immunoglobulin E (IgE) is a heterodimeric glycoprotein involved in antiparasitic and allergic immune reactions. 10861210 1 29 gly N-glycosylated 367:380 arg1 AE2 AE2 PUBTATOR AE2 6522 The human erythrocyte anion exchanger (AE)1 (Band 3) contains a single complex N-linked oligosaccharide that is attached to Asn(642) in the fourth extracellular loop of this polytopic membrane protein, while other isoforms (AE2, AE3 and trout AE1) are N-glycosylated on the preceding extracellular loop. 10861210 1 29 gly N-glycosylated 367:380 arg1 AE3 AE3 PUBTATOR AE3 6508 The human erythrocyte anion exchanger (AE)1 (Band 3) contains a single complex N-linked oligosaccharide that is attached to Asn(642) in the fourth extracellular loop of this polytopic membrane protein, while other isoforms (AE2, AE3 and trout AE1) are N-glycosylated on the preceding extracellular loop. 16321355 8 17 gly linked 1362:1367 arg2 alpha1-3 AND fucose residues alpha1-3 fucose residues PUBTATOR alpha1-3 146 Exoglycosidase digestion suggested that fucose residues were linked to reducing end GlcNAc in biantennary oligosaccharides and to reducing end and/or alpha1-3 to outer arms GlcNAc in triantennary oligosaccharides and that roughly one of the antennas in triantennary oligosaccharides was alpha2-3 sialylated and occasionally alpha1-3 fucosylated at GlcNAc. 9884403 0 15 gly glycoprotein 75:86 arg1 human Tamm-Horsfall glycoprotein human Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 Glycosylation sites and site-specific glycosylation in human Tamm-Horsfall glycoprotein. 9884403 0 67 gly glycosylation 38:50 arg1 human Tamm-Horsfall glycoprotein human Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 Glycosylation sites and site-specific glycosylation in human Tamm-Horsfall glycoprotein. 21805521 5 27 gly glycosylated 830:841 arg1 huDKK1 huDKK1 Cterm huDKK1 22943 LC-MS/MS peptide mapping indicates that ∼ 92% of huDKK1 is glycosylated at Asn²²⁵ with three N-linked glycans composed of two biantennary forms with 1 and 2 sialic acid (23% and 60%, respectively), and one triantennary structure with 2 sialic acids (9%). 8174273 2 28 gly desialylated 358:369 arg1 desialylated and pepsin-digested transferrin desialylated and pepsin-digested transferrin PUBTATOR transferrin 7018 Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. 8174273 2 53 gly released 327:334 arg2 desialylated and pepsin-digested transferrin AND Sugar chains desialylated and pepsin-digested transferrin Sugar chains PUBTATOR transferrin 7018 Sugar chains released with N-glycanase from desialylated and pepsin-digested transferrin were derivatized by reductive pyridylamination. 15616124 6 12 gly N-glycosylation 908:922 arg1 FVII FVII Cterm FVII 2155 Immediately after pulse, most labeled intracellular FVII had one N-glycan, but during a 1-h chase, the vast majority was processed into FVII with two N-glycans, demonstrating posttranslational N-glycosylation of FVII. 15616124 6 79 gly FVII 851:854 arg1 two N-glycans FVII two N-glycans Cterm FVII 2155 Immediately after pulse, most labeled intracellular FVII had one N-glycan, but during a 1-h chase, the vast majority was processed into FVII with two N-glycans, demonstrating posttranslational N-glycosylation of FVII. 15616124 6 39 gly had 772:774 arg1 most labeled intracellular FVII AND one N-glycan most labeled intracellular FVII one N-glycan Cterm FVII 2155 Immediately after pulse, most labeled intracellular FVII had one N-glycan, but during a 1-h chase, the vast majority was processed into FVII with two N-glycans, demonstrating posttranslational N-glycosylation of FVII. 32366695 3 35 gly SARS-CoV-2 356:365 arg1 22 N-linked glycan sequons SARS 22 N-linked glycan sequons OGER SARS P49591 SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. 1823160 1 3 gly activator 303:311 arg1 the N-linked oligosaccharides tissue plasminogen activator the N-linked oligosaccharides OGER tissue plasminogen activator P00750 We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). 16834341 7 2 part_of hFSH 964:967 arg1 Asn52 hFSH Asn52 OGER AminoAcid hFSH Asn52 Also, glycoforms at Asn52 of hFSH are all complex type, whereas in eFSH, both complex and hybrid structures exist at this site. 23050552 1 55 gly glycoprotein 132:143 arg1 Human fibrinogen Human fibrinogen PUBTATOR Human fibrinogen 2244 Human fibrinogen is a 340 kDa, soluble plasma glycoprotein composed of paired sets of three subunits (α, β, γ). 16859706 4 9 gly bears 644:648 arg1 TPST1 AND N-linked glycosyl residues TPST1 position Asn60 and Asn262 N-linked glycosyl residues PUBTATOR AminoAcid TPST1 8460 position Asn60 and Asn262 PNGase treatment and mutational studies determined that TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262. 20739279 12 34 gly N-glycosylation 1524:1538 arg1 SynCAM proteins SynCAM proteins PUBTATOR SynCAM proteins 23705 These results demonstrate that N-glycosylation of SynCAM proteins differentially affects their binding interface and implicate post-translational modification as a mechanism to regulate trans-synaptic adhesion. 22159084 10 58 gly Deglycosylation 1249:1263 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 Deglycosylation of SLC26A3 causes a defect in cell surface processing with decreased cell surface expression. 1381905 6 32 gly glycosylated 1048:1059 arg1 cell-associated glycosylated SCF cell-associated glycosylated SCF OGER SCF P21583 Pulse-labeling of cells with 35S-labeled Met and Cys resulted in cell-associated glycosylated SCF of M(r) 33,000-45,000 which was converted to M(r) 33,000 by in vitro treatment with glycosidases. 1533633 11 23 gly nonglycosylated 1656:1670 arg1 the nonglycosylated, misfolded alpha-subunit the nonglycosylated, misfolded alpha-subunit OGER subunit P06865 The properties of the nonglycosylated, misfolded alpha-subunit were similar to some mutant alpha-subunits in Tay-Sachs disease patients. 2033371 8 10 gly oligosaccharide 1615:1629 arg1 CD43 CD43 oligosaccharide PUBTATOR CD43 6693 Only after activation NeuNAc alpha 2----3Gal beta 1----3 (NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6) GalNAcOH becomes the principal oligosaccharide on CD43 from normal lymphocytes. 7599134 2 9 gly has 343:345 arg1 The alpha-ATIII isoform AND four N-linked oligosaccharides The alpha-ATIII isoform four N-linked oligosaccharides PUBTATOR ATIII isoform 462 The alpha-ATIII isoform has four N-linked oligosaccharides attached to asparagines 96, 135, 155, and 192. 8981093 4 63 gly EPO 988:990 arg1 sialic acid content EPO sialic acid content PUBTATOR EPO 2056 On the other hand, an inverse relationship between the in vitro bioactivity and sialic acid content of EPO was observed. 9169007 6 51 gly variants 782:789 arg1 Heparin-agarose chromatography antithrombin variants Heparin-agarose chromatography PUBTATOR antithrombin variants 462 Heparin-agarose chromatography of the four antithrombin variants revealed that Gln 96, Gln 135, and Gln 192 variants still displayed the two functional heparin-affinity forms previously observed with the wild-type inhibitor, whereas the Gln 155 variant showed only a single functional high heparin affinity form. 2113054 6 5 gly glycosylation 1171:1183 arg1 sCD4 sCD4 PUBTATOR sCD4 499358 The role of glycosylation in the expression of sCD4 was investigated by mutagenesis of the constructs to remove each of the two N-linked glycosylation sites in turn and both together. 23269669 0 80 gly glycosylation 23:35 arg1 receptor guanylyl cyclase C receptor guanylyl cyclase C PUBTATOR guanylyl cyclase C 2984 Site-specific N-linked glycosylation of receptor guanylyl cyclase C regulates ligand binding, ligand-mediated activation and interaction with vesicular integral membrane protein 36, VIP36. 23269669 5 15 gly glycosylation 902:914 arg1 human GC-C human GC-C PUBTATOR GC-C 2984 When glycosylation of human GC-C was prevented, either by pharmacological intervention or by mutation of all of the 10 predicted glycosylation sites, ST binding and surface localization was abolished. 12144777 1 21 gly glycoprotein 125:136 arg1 Human renal dipeptidase Human renal dipeptidase PUBTATOR renal dipeptidase 1800 Human renal dipeptidase is a membrane-bound glycoprotein hydrolyzing dipeptides and is involved in hydrolytic metabolism of penem and carbapenem beta-lactam antibiotics. 3934016 3 8 gly nonglycosylated 573:587 arg1 angiotensinogen angiotensinogen PUBTATOR angiotensinogen 183 35S-Methionine-labeled precursor and processed forms of angiotensinogen were compared with glycosylated and nonglycosylated 35S-methionine-labeled mature forms of angiotensinogen secreted by hepatoma cells, using immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. 3934016 3 14 gly glycosylated 556:567 arg1 angiotensinogen angiotensinogen PUBTATOR angiotensinogen 183 35S-Methionine-labeled precursor and processed forms of angiotensinogen were compared with glycosylated and nonglycosylated 35S-methionine-labeled mature forms of angiotensinogen secreted by hepatoma cells, using immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. 18491227 8 40 gly E-cadherin 1305:1314 arg1 N-glycans E-cadherin N-glycans PUBTATOR E-cadherin 999 Furthermore, we found that N-glycans of M4 E-cadherin were modified in immature high mannose type, suggesting that it could not depart to Golgi apparatus. 11544325 7 19 gly glycosylation 1198:1210 arg1 hMD-2 hMD-2 PUBTATOR hMD-2 23643 These observations demonstrate that hMD-2 undergoes N-linked glycosylation at Asn(26) and Asn(114), and that these glycosylations are crucial for TLR4-mediated signal transduction of LPS. 9334252 1 1 gly urine 168:172 arg1 RNase 2 RNase 2 urine PUBTATOR RNase 2 6036 C2-alpha-Mannosyltryptophan was discovered in RNase 2 from human urine, representing a novel way of attaching carbohydrate to a protein. 11788899 7 22 part_of WNT8B 910:914 arg1 Gly230 WNT8B Gly230 and Arg284 PUBTATOR AminoAcid WNT8B 7479 Gly230 and Arg284 Gly230 and Arg284 of WNT8B were conserved in WNT8A. 11904304 3 0 part_of Thr-58 677:682 arg1 c-Myc c-Myc Thr-58 PUBTATOR SpecificSite c-Myc 4609 Thr-58 One antibody specifically reacts with the Thr-58-glycosylated form of c-Myc, and the other reacts only with unmodified Thr-58 in c-Myc. 26271046 11 76 gly N-glycans 1912:1920 arg1 GluA2 GluA2 N-glycans PUBTATOR GluA2 2891 Thus, our data suggested that site-specific N-glycans on GluA2 regulate the intracellular trafficking and cell surface expression of AMPAR. 14658030 0 82 gly N-glycosylation 18:32 arg1 the murine neural cell adhesion molecule NCAM the murine neural cell adhesion molecule NCAM PUBTATOR NCAM 17967 Identification of N-glycosylation sites of the murine neural cell adhesion molecule NCAM by MALDI-TOF and MALDI-FTICR mass spectrometry. 22121020 0 23 gly H2B 25:27 arg1 GlcNAcylation histone H2B GlcNAcylation PUBTATOR histone H2B 3772104 GlcNAcylation of histone H2B facilitates its monoubiquitination. 2808371 1 6 gly possesses 174:182 arg1 Erythrocyte protein 4.1 AND a protein-saccharide modification structure Erythrocyte protein 4.1 a protein-saccharide modification structure PUBTATOR protein 4.1 281753 Erythrocyte protein 4.1 is a cytoplasmic protein that possesses a protein-saccharide modification structure, an O-N-acetyl-D-glucosamine (GlcNAc) moiety. 2808371 1 6 gly possesses 174:182 arg1 Erythrocyte protein 4.1 AND an O-N-acetyl-D-glucosamine (GlcNAc) moiety Erythrocyte protein 4.1 an O-N-acetyl-D-glucosamine (GlcNAc) moiety PUBTATOR protein 4.1 281753 Erythrocyte protein 4.1 is a cytoplasmic protein that possesses a protein-saccharide modification structure, an O-N-acetyl-D-glucosamine (GlcNAc) moiety. 21932778 9 45 gly glycans 1714:1720 arg1 glypican-1 glypican-1 glycans PUBTATOR glypican-1 2817 In summary, we have found that the potential N-glycosylation sites in glypican-1 are invariably occupied and that the N-linked glycans on glypican-1 affect protein expression and heparan sulfate substitution but that correct folding can be obtained in the absence of N-linked glycans. 10413465 5 42 gly O-glycosylation 1142:1156 arg1 Thr194-Ala Thr194-Ala Cterm Thr194-Ala 348 This analysis showed that a mutation in the O-glycosylation site of apoE2 (Thr194-Ala) did not affect the SDS-stable binding of apoE to Abeta. 10413465 5 42 gly O-glycosylation 1142:1156 arg1 apoE2 apoE2 PUBTATOR apoE2 348 This analysis showed that a mutation in the O-glycosylation site of apoE2 (Thr194-Ala) did not affect the SDS-stable binding of apoE to Abeta. 14749323 10 96 gly glycosylation 1816:1828 arg1 OAT1 function OAT1 function PUBTATOR OAT1 9356 In summary, we provided the evidence that 1) Asp-39 is crucially involved in substrate recognition of OAT1, 2) glycosylation at individual sites is not required for OAT1 function, and 3) glycosylation plays an important role in the targeting of OAT1 onto the plasma membrane. 20484118 4 6 part_of PLN 697:699 arg1 Ser PLN Ser(16) PUBTATOR SpecificSite PLN 64672 Ser(16) Studies using enzymatic labeling and co-immunoprecipitation of wild type and a series of mutants of PLN showed that PLN was O-GlcNAcylated and Ser(16) of PLN might be the site for O-GlcNAcylation. 7776966 7 102 gly glycosylation 1056:1068 arg1 the FSHR as well as to elucidate their role in the functions of the FSHR the FSHR as well as to elucidate their role in the functions of the FSHR PUBTATOR FSHR 2492 Therefore, further experiments, done in the context of the full-length receptor, were performed to determine the actual sites of glycosylation in the FSHR as well as to elucidate their role in the functions of the FSHR. 16368742 0 76 gly Glycosylation 0:12 arg1 the osmoresponsive transient receptor potential channel TRPV4 the osmoresponsive transient receptor potential channel TRPV4 PUBTATOR TRPV4 59341 Glycosylation of the osmoresponsive transient receptor potential channel TRPV4 on Asn-651 influences membrane trafficking. 8344278 3 31 gly A 587:587 arg1 the oligosaccharide moieties saposin A the oligosaccharide moieties Cterm saposin A The characteristic features of the oligosaccharide moieties of saposin A are (a) the predominance of a fucosylated trimannosyl core structure and (b) the occurrence of several different oligomannose-type and N-acetyllactosamine-type oligosaccharides. 30127001 2 32 gly modification 273:284 arg1 serine 435 serine 435 serine 435 SpecificSite serine 435 A recent structural analysis suggested that a novel O-linked hexose modification on serine 435 of the mammalian NOTCH1 core ligand-binding domain lies at the interface with its ligands. 19864504 0 73 gly A 91:91 arg1 N-linked oligosaccharides arylsulfatase A N-linked oligosaccharides PUBTATOR arylsulfatase A 410 Site-specific analysis of N-linked oligosaccharides of recombinant lysosomal arylsulfatase A produced in different cell lines. 21669976 7 25 gly glycoforms 1272:1281 arg1 the different KCNE1 glycoforms the different KCNE1 glycoforms PUBTATOR KCNE1 3753 The enzymatic assays and panel of glycosylation mutants used here will be valuable for identifying the different KCNE1 glycoforms in native cells and determining the roles N- and O-glycosylation play in KCNQ1–KCNE1 function and localization in cardiomyocytes, 18420026 0 60 gly glycoprotein 28:39 arg1 Nectin-like molecule 1 Nectin-like molecule 1 PUBTATOR Nectin-like molecule 1 57863 Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity. 8407981 2 26 gly glycosylated 215:226 arg1 cytochrome P-450 cytochrome P-450(arom) PUBTATOR cytochrome P-450(arom) 55010 It was found that cytochrome P-450(arom) purified from human placenta microsomes is glycosylated, and the sugar chain was cleaved with endoglycosidase H (Endo H). 26467158 9 36 gly GlcNAc 1556:1561 arg1 BACE1 BACE1 GlcNAc PUBTATOR BACE1 23821 These mutations almost cancelled the enhanced BACE1 degradation seen in Mgat3(-/-) MEFs, indicating that bisecting GlcNAc on BACE1 indeed regulates its degradation. 24161696 9 1 gly Kv3.1b 1722:1727 arg1 N-glycans Kv3.1b N-glycans Cterm Kv3.1b GENERAL SIGNIFICANCE: Our study demonstrates that N-glycans of Kv3.1b contain information regarding the association, clustering, and distribution of Kv3.1b in the cell membrane, and furthermore that decreased occupancy caused by congenital disorders of glycosylation may alter the biological activity of Kv3.1b. 2493268 1 36 gly O-glycosylated 128:141 arg1 The 5-oxoproline N-terminal chain The 5-oxoproline N-terminal chain OGER chain The 5-oxoproline N-terminal chain is O-glycosylated on the 5th amino acid residue. 16877748 6 18 gly carried 1133:1139 arg1 sICAM-1 AND trisialylated complex-type N-glycans sICAM-1 trisialylated complex-type N-glycans Cterm sICAM-1 3383 Ion-exchange chromatography and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) of the released N-glycans showed that sICAM-1 mostly carried di- and trisialylated complex-type N-glycans with or without one fucose. 16877748 6 18 gly carried 1133:1139 arg1 sICAM-1 AND di- sICAM-1 di- Cterm sICAM-1 3383 Ion-exchange chromatography and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) of the released N-glycans showed that sICAM-1 mostly carried di- and trisialylated complex-type N-glycans with or without one fucose. 24125761 11 67 gly glycosylation 1573:1585 arg1 AADAC AADAC PUBTATOR AADAC 13 The mutant types of AADAC containing the N282Q and the N78Q/N282Q substitutions were not detected by immunoblotting analysis after non-reducing SDS-PAGE, suggesting that the glycosylation of AADAC at N282 was important for proper protein folding. 16877748 10 31 gly glycosylated 1997:2008 arg1 mouse sICAM-1 mouse sICAM-1 Cterm sICAM-1 3383 These results show that the N-glycans that enhance the MIP-2-inducing activity of mouse sICAM-1 are mostly di- and trisialylated complex-type N-glycans including a small fraction carrying more sialic acid residues than antennae and that the nine N-glycosylation sites of mouse sICAM-1 are all glycosylated. 16877748 10 67 gly N-glycosylation 1950:1964 arg1 mouse sICAM-1 mouse sICAM-1 Cterm sICAM-1 3383 These results show that the N-glycans that enhance the MIP-2-inducing activity of mouse sICAM-1 are mostly di- and trisialylated complex-type N-glycans including a small fraction carrying more sialic acid residues than antennae and that the nine N-glycosylation sites of mouse sICAM-1 are all glycosylated. 22967898 2 17 gly glycosylated 324:335 arg1 cathepsin V cathepsin V PUBTATOR cathepsin V 1515 Cathepsin V contains two predicted N-glycosylation sites, but it has not been reported whether cathepsin V is glycosylated or not. 12408961 6 41 gly N-glycosylated 816:829 arg1 Human and mouse CREG2 Human and mouse CREG2 PUBTATOR CREG2 263764 Human and mouse CREG2 are N-glycosylated in HeLa cells and deletion of amino-terminal sequences completely abolishes N-glycosylation, indicating that the N termini of both proteins may function as signal sequences. 11544325 4 47 part_of MD-2 628:631 arg1 Asn(26) MD-2 Asn(26) and Asn(114) PUBTATOR SpecificSite MD-2 23643 Asn(26) and Asn(114) Mutation of either one of two potential glycosylation sites (Asn(26) and Asn(114)) of MD-2 resulted in the disappearance of the slowest mobility form, and only the fastest form was detected in hMD-2 carrying mutations at both Asn(26) and Asn(114). 2015821 7 53 gly contains 1348:1355 arg1 1 AND one diantennary glycans 1 one diantennary glycans PUBTATOR peak 1 79834 Moreover our results show that the peak 1 contains four triantennary glycans, the peak 2 three triantennary and one diantennary glycans while the bound peaks 3 + 4 possess, on average, about one triantennary and three diantennary glycans per molecule. 2015821 7 53 gly contains 1348:1355 arg1 1 AND four triantennary glycans 1 four triantennary glycans PUBTATOR peak 1 79834 Moreover our results show that the peak 1 contains four triantennary glycans, the peak 2 three triantennary and one diantennary glycans while the bound peaks 3 + 4 possess, on average, about one triantennary and three diantennary glycans per molecule. 1731338 3 58 gly galactoglycoprotein 606:624 arg1 the native galactoglycoprotein the native galactoglycoprotein PUBTATOR galactoglycoprotein 6693 Comparison of peptide sequences from the native galactoglycoprotein and the deglycosylated derivative demonstrated the locations of 25 sites of O-glycosylation and three serine sites that are not glycosylated. 28668641 0 25 gly Glycosylation 0:12 arg1 SERPINA12 SERPINA12 PUBTATOR SERPINA12 145264 Glycosylation of human vaspin (SERPINA12) and its impact on serpin activity, heparin binding and thermal stability. 28668641 0 25 gly Glycosylation 0:12 arg1 human vaspin human vaspin PUBTATOR vaspin 145264 Glycosylation of human vaspin (SERPINA12) and its impact on serpin activity, heparin binding and thermal stability. 16169851 6 14 gly glycoprotein 767:778 arg1 herpes virus glycoprotein herpes virus glycoprotein PUBTATOR glycoprotein D 2532 Moreover, the structure shows that BTLA recognizes the same surface on HVEM as gD (herpes virus glycoprotein D) and utilizes a similar binding motif. 2403553 1 62 gly oligosaccharides 88:103 arg1 the human transferrin receptor transferrin receptor oligosaccharides PUBTATOR transferrin receptor 7018 We have investigated the oligosaccharides in the human transferrin receptor from three different cell lines. 28450392 8 11 gly NSL3 1347:1350 arg1 O-GlcNAcylation NSL3 O-GlcNAcylation PUBTATOR NSL3 55683 Moreover, O-GlcNAcylation of NSL3 by wild-type OGT1 (OGT1-WT) stabilized NSL3. 24884609 9 6 gly contained 1294:1302 arg1 recombinant ITIH4 AND high-mannose N-linked glycans recombinant ITIH4 high-mannose N-linked glycans PUBTATOR ITIH4 3700 Site N274 contained high-mannose N-linked glycans in both serum and recombinant ITIH4. 7613477 3 117 gly glycopeptides 793:805 arg1 LCAT LCAT PUBTATOR LCAT 3931 In addition to the four expected N-linked glycopeptides of LCAT, a di-O-linked glycopeptide was detected, as well as three additional glycopeptides. 17563389 1 65 part_of has 226:228 arg1 neurokinin 1 receptor AND Asn-14 neurokinin 1 receptor sites, Asn-14 and Asn-18 PUBTATOR SpecificSite neurokinin 1 receptor 6869 sites, Asn-14 and Asn-18 The neurokinin 1 receptor (NK1R), a G protein-coupled receptor involved in diverse functions including pain and inflammation, has two putative N-linked glycosylation sites, Asn-14 and Asn-18. 17563389 1 65 part_of has 226:228 arg1 neurokinin 1 receptor AND Asn-18 neurokinin 1 receptor sites, Asn-14 and Asn-18 PUBTATOR SpecificSite neurokinin 1 receptor 6869 sites, Asn-14 and Asn-18 The neurokinin 1 receptor (NK1R), a G protein-coupled receptor involved in diverse functions including pain and inflammation, has two putative N-linked glycosylation sites, Asn-14 and Asn-18. 17563389 1 65 part_of has 226:228 arg1 neurokinin 1 receptor AND Asn-18 neurokinin 1 receptor sites, Asn-14 and Asn-18 PUBTATOR SpecificSite neurokinin 1 receptor 6869 sites, Asn-14 and Asn-18 The neurokinin 1 receptor (NK1R), a G protein-coupled receptor involved in diverse functions including pain and inflammation, has two putative N-linked glycosylation sites, Asn-14 and Asn-18. 17563389 1 65 part_of has 226:228 arg1 NK1R AND Asn-14 NK1R sites, Asn-14 and Asn-18 PUBTATOR SpecificSite NK1R 6869 sites, Asn-14 and Asn-18 The neurokinin 1 receptor (NK1R), a G protein-coupled receptor involved in diverse functions including pain and inflammation, has two putative N-linked glycosylation sites, Asn-14 and Asn-18. 17563389 1 65 part_of has 226:228 arg1 NK1R AND Asn-18 NK1R sites, Asn-14 and Asn-18 PUBTATOR SpecificSite NK1R 6869 sites, Asn-14 and Asn-18 The neurokinin 1 receptor (NK1R), a G protein-coupled receptor involved in diverse functions including pain and inflammation, has two putative N-linked glycosylation sites, Asn-14 and Asn-18. 17563389 1 65 part_of has 226:228 arg1 NK1R AND Asn-18 NK1R sites, Asn-14 and Asn-18 PUBTATOR SpecificSite NK1R 6869 sites, Asn-14 and Asn-18 The neurokinin 1 receptor (NK1R), a G protein-coupled receptor involved in diverse functions including pain and inflammation, has two putative N-linked glycosylation sites, Asn-14 and Asn-18. 12545205 3 88 gly glycosylated 393:404 arg1 N-cadherin N-cadherin PUBTATOR N-cadherin 1000 N-cadherin is a heavily glycosylated protein. 1823160 7 33 gly structures 1076:1085 arg1 rmt-PA mt-PA structures Cterm mt-PA The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. 16321355 2 48 gly glycosylation 270:282 arg1 CP CP PUBTATOR CP 1356 In this study, a site-specific glycosylation analysis of human ceruloplasmin (CP) was carried out using reversed-phase high-performance liquid chromatography with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). 16321355 2 48 gly glycosylation 270:282 arg1 human ceruloplasmin human ceruloplasmin PUBTATOR ceruloplasmin 1356 In this study, a site-specific glycosylation analysis of human ceruloplasmin (CP) was carried out using reversed-phase high-performance liquid chromatography with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). 14573609 4 18 gly glycosylated 578:589 arg1 Heparanase protein Heparanase protein PUBTATOR Heparanase protein 10855 Heparanase protein was glycosylated at six Asn residues in human tumor cell lines. 2514791 4 23 part_of t-PA 1495:1498 arg1 Asn-448 t-PA Asn-448 PUBTATOR SpecificSite t-PA 5327 Asn-448 Our results suggest the following: (i) type I and type II t-PA are N-glycosylated in an identical way at Asn-117 and Asn-448, when isolated from the same cell line; (ii) Asn-117 is predominantly associated with oligomannose-type structures in all cases; (iii) Asn-184 and Asn-448 are predominantly associated with complex-type structures when t-PA is isolated from fibroblast cells, but with both complex- and oligomannose-type structures when isolated from melanoma cells; (iv) fibroblast cell derived t-PA is associated with both neutral and sialylated oligosaccharides, while melanoma cell derived t-PA is also associated with sulfated oligosaccharides, which are located exclusively at Asn-448 of type II t-PA; (v) no complex-type structures occur in common between t-PA from the two cell lines. 25760607 6 38 gly glycosylated 750:761 arg1 glycosylated LLT1 glycosylated LLT1 PUBTATOR LLT1 29121 The hexamer of glycosylated LLT1 consists of three classical dimers. 17956937 3 48 gly PSA 610:612 arg1 the glycan profiles PSA the glycan profiles PUBTATOR PSA 354 In the present study, the glycan profiles of free and complexed forms of PSA from cancer patient serum and of seminal plasma PSA were compared by analyzing the glycopeptides obtained by lysylendopeptidase digestion of the electrophoretically separated PSA with mass spectrometry. 17956937 3 73 gly PSA 558:560 arg1 free and complexed forms PSA free and complexed forms PUBTATOR PSA 354 In the present study, the glycan profiles of free and complexed forms of PSA from cancer patient serum and of seminal plasma PSA were compared by analyzing the glycopeptides obtained by lysylendopeptidase digestion of the electrophoretically separated PSA with mass spectrometry. 22745122 7 14 part_of serine 884:889 arg1 DRP1 DRP1 serine PUBTATOR SpecificSite DRP1 10059 serine 637 Increased O-GlcNAcylation decreases the phosphorylation of DRP1 at serine 637, which is known to regulate DRP1 function. 9767079 3 50 gly glycosylated 545:556 arg1 glycosylated RFC glycosylated RFC PUBTATOR RFC 6573 At 3 microg/ml tunicamycin, the nearly complete loss of glycosylated RFC was accompanied by a approximately 25% decreased rate of methotrexate uptake. 11544325 4 9 gly glycosylation 582:594 arg1 MD-2 MD-2 PUBTATOR MD-2 23643 Mutation of either one of two potential glycosylation sites (Asn(26) and Asn(114)) of MD-2 resulted in the disappearance of the slowest mobility form, and only the fastest form was detected in hMD-2 carrying mutations at both Asn(26) and Asn(114). 7642555 2 4 gly modified 337:344 arg1 c-Myc AND O-linked N-acetylglucosamine c-Myc O-linked N-acetylglucosamine PUBTATOR c-Myc 4609 Previously, we demonstrated that c-Myc is modified by O-linked N-acetylglucosamine (O-GlcNAc) within or nearby the N-terminal transcriptional activation domain (Chou, T.-Y., Dang, C.V., and Hart, G.W. (1995) Proc. 7642555 2 4 gly modified 337:344 arg1 c-Myc AND O-GlcNAc c-Myc O-GlcNAc PUBTATOR c-Myc 4609 Previously, we demonstrated that c-Myc is modified by O-linked N-acetylglucosamine (O-GlcNAc) within or nearby the N-terminal transcriptional activation domain (Chou, T.-Y., Dang, C.V., and Hart, G.W. (1995) Proc. 1694179 0 45 gly glycosylated 22:33 arg1 Beta protein C Beta protein C Cterm Beta protein C Beta protein C is not glycosylated at asparagine 329. 9767079 7 33 gly N-glycosylation 1274:1288 arg1 human RFC human RFC PUBTATOR RFC 6573 Collectively, our results demonstrate that N-glycosylation of human RFC plays no significant role in either transport function or membrane targeting. 11912203 5 63 gly glycosylated 658:669 arg1 the adiponectin isoforms the adiponectin isoforms PUBTATOR adiponectin isoforms 9370 Carbohydrate detection revealed that six of the adiponectin isoforms are glycosylated. 14764083 2 68 gly glycosylation 232:244 arg1 LF LF PUBTATOR LF 3131 To elucidate the role of N-linked glycosylation in protection of LF against proteolysis, we compared the tryptic susceptibility of human LF (hLF) variants from human milk, expressed in human 293(S) cells or in the milk of transgenic mice and cows. 6203908 7 70 part_of subunit 660:666 arg1 Cys-447 subunit Cys-447 OGER SpecificSite subunit 2 Cys-447 Cys-447 probably forms an interchain bridge with Cys-447 from another subunit. 27314333 11 55 gly N-glycosylation 1736:1750 arg1 Rspo1 Rspo1 PUBTATOR Rspo1 284654 Our findings provide evidence for the critical role of N-glycosylation in the biogenesis of Rspo1. 7106126 0 52 gly sialoglycoprotein 34:50 arg1 sialoglycoprotein D sialoglycoprotein D PUBTATOR sialoglycoprotein D 2995 N-terminal amino acid sequence of sialoglycoprotein D (glycophorin C) from human erythrocyte membranes. 18533687 5 86 gly glycosylation 797:809 arg1 RXFP1 RXFP1 PUBTATOR RXFP1 59350 We herein investigate the actual N-linked glycosylation status of RXFP1 and the functional ramifications of these post-translational modifications. 7514386 0 31 gly form 112:115 arg1 the asparagine-linked sugar chain form of CD59 the asparagine-linked sugar chain PUBTATOR form of CD59 966 Structural study on the glycosyl-phosphatidylinositol anchor and the asparagine-linked sugar chain of a soluble form of CD59 in human urine. 3458201 1 17 gly 1B-glycoprotein 171:185 arg1 alpha 1B-glycoprotein alpha 1B-glycoprotein PUBTATOR alpha 1B-glycoprotein 1 The complete amino acid sequence has been determined for alpha 1B-glycoprotein (alpha 1B), a protein of unknown function present in human plasma. 3458201 1 17 gly 1B-glycoprotein 171:185 arg1 alpha 1B alpha 1B PUBTATOR alpha 1B 1 The complete amino acid sequence has been determined for alpha 1B-glycoprotein (alpha 1B), a protein of unknown function present in human plasma. 11502179 1 68 gly containing 283:292 arg1 the human TSH receptor AND a 10-residue histidine tag the human TSH receptor The amino-terminal ectodomain a 10-residue histidine tag PUBTATOR TSH receptor 7253 ectodomain The amino-terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a glycosylphosphatidylinositol-anchored molecule containing a 10-residue histidine tag close to its C terminus. 17768338 2 38 gly glycoprotein 230:241 arg1 BChE BChE PUBTATOR BChE 590 BChE is a 340 kDa tetrameric glycoprotein that is present in human serum at a concentration of 5 mg l(-1). 17534424 9 80 gly glycoprotein 2188:2199 arg1 human ClC-6 human ClC-6 OGER ClC Q05315 CONCLUSIONS: We conclude that human ClC-6 is an endosomal glycoprotein that partitions in detergent resistant lipid domains. 9054441 0 0 part_of plasminogen 75:85 arg1 Ser-248 plasminogen 2 Ser-248 OGER SpecificSite plasminogen 2 P00747 Ser-248 Evidence for a novel O-linked sialylated trisaccharide on Ser-248 of human plasminogen 2. 15616124 4 68 gly FVII 541:544 arg1 one and two N-glycans FVII one and two N-glycans Cterm FVII 2155 Pulse-chase labeled intracellular FVII migrated as two bands corresponding to FVII with one and two N-glycans, respectively. 17980170 2 0 gly glycosylated 356:367 arg1 NAAA NAAA PUBTATOR NAAA 27163 Previously, we suggested that NAAA is glycosylated and proteolytically cleaved. 2129367 5 69 part_of IX 639:640 arg1 Ser-53 factor IX Ser-53 OGER SpecificSite factor IX P00740 Ser-53 The same results were also obtained for a pentapeptide containing Ser-53 of factor IX and protein Z. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. 10441114 0 43 gly glycosylated 6:17 arg1 human plasma endostatin human plasma endostatin PUBTATOR endostatin 80781 Novel glycosylated forms of human plasma endostatin and circulating endostatin-related fragments of collagen XV. 2514791 5 7 gly glycoprotein 1623:1634 arg1 the t-PA glycoprotein the t-PA glycoprotein PUBTATOR t-PA glycoprotein 5327 These results indicate that the t-PA glycoprotein is secreted by each cell line as a set of glycoforms, each glycoform being unique with respect to the nature and disposition of oligosaccharides on a common polypeptide. 22448645 0 64 gly glycosylation 50:62 arg1 the human serotonin 5-HT₇a receptor the human serotonin 5-HT₇a receptor Cterm 5-HT₇a Biochemical and pharmacological study of N-linked glycosylation of the human serotonin 5-HT₇a receptor. 23269669 6 16 gly glycosylation 1148:1160 arg1 GC-C GC-C PUBTATOR GC-C 2984 Systematic mutagenesis of each of the 10 sites of glycosylation in GC-C, either singly or in combination, identified two sites that were critical for ligand binding and two that regulated ST-mediated activation. 25374123 2 78 gly glycoprotein 333:344 arg1 Vitronectin Vitronectin PUBTATOR Vitronectin 7448 Vitronectin is well known to be a multifunctional glycoprotein in the blood and the extracellular matrix, which is related to hepatocellular carcinoma (HCC). 19690161 2 71 gly glycosylation 342:354 arg1 huIL-6 (1) huIL-6 (1) PUBTATOR IL-6 3569 Our previous work showed that N-linked glycosylation was required for optimal function of vIL6 but not huIL-6 (1). 19556306 7 26 gly TLR4 1167:1170 arg1 high mannose type TLR4 high mannose type PUBTATOR TLR4 7099 alpha-Mannosidase II inhibitor blocked the processing N-glycans to complex type, but TLR4 with high mannose type appeared on the cell surface, suggesting that TLR4 is destined to locate on the cell surface before processing N-glycans from a high mannose type to a complex type. 16368742 5 83 part_of TRPV4 974:978 arg1 Residue N651 TRPV4 Residue N651 PUBTATOR SpecificSite TRPV4 59341 N651 Residue N651 of TRPV4 is immediately adjacent to the pore-forming loop. 12590919 3 18 gly glycosylated 480:491 arg1 hENT2 hENT2 PUBTATOR hENT2 3177 We address these questions using N-glycosylation mutants (N48D, N57D, and N48/57D) and demonstrate that hENT2 is glycosylated at Asn(48) and Asn(57). 21264968 6 74 part_of MUC4 2024:2027 arg1 Thr10 MUC4 Thr10 PUBTATOR AminoAcid MUC4 4585 Thr10 The solution structures determined by NMR spectroscopic studies elicited that the preferential introduction of α-GalNAc at Thr10 of MUC4 stabilizes specifically a β-like extended backbone structure at this area, whereas other synthetic models with a single α-GalNAc residue at Thr1, Thr6, or Thr15 did not exhibit any converged three-dimensional structure at the proximal peptide moiety. 24365146 5 11 gly glycosylation 772:784 arg1 Nox1 Nox1 PUBTATOR Nox1 27035 These findings indicate that glycosylation and subsequent N-glycan maturation of Nox1 are both dispensable for its cell surface recruitment. 24365146 5 14 gly Nox1 824:827 arg1 subsequent N-glycan maturation Nox1 subsequent N-glycan maturation PUBTATOR Nox1 27035 These findings indicate that glycosylation and subsequent N-glycan maturation of Nox1 are both dispensable for its cell surface recruitment. 1737041 2 21 gly contains 395:402 arg1 Human GM-CSF AND two N-linked carbohydrate side chains Human GM-CSF two N-linked carbohydrate side chains PUBTATOR Human GM-CSF 1437 Human GM-CSF contains two N-linked carbohydrate side chains of the complex acidic type and several sites of O-linked carbohydrate clustered on serine and threonine residues near the N-terminus of the molecule. 7535613 11 62 part_of PSA 1606:1608 arg1 The PSA glycosylation site PSA site, Asn 61 PUBTATOR SpecificSite PSA P07288 site, Asn 61 The PSA glycosylation site, Asn 61, is fully accessible to the solvent and is enclosed in a positive region of the isopotential map. 1374031 0 45 gly subunit 36:42 arg1 The carbohydrate chains subunit The carbohydrate chains OGER subunit P0DN86 The carbohydrate chains of the beta subunit of human chorionic gonadotropin produced by the choriocarcinoma cell line BeWo. 7599134 0 35 part_of antithrombin 25:36 arg1 antithrombin III asparagine-135 antithrombin III antithrombin III asparagine-135 PUBTATOR SpecificSite antithrombin III 462 asparagine-135 Partial glycosylation of antithrombin III asparagine-135 is caused by the serine in the third position of its N-glycosylation consensus sequence and is responsible for production of the beta-antithrombin III isoform with enhanced heparin affinity. 10712595 6 53 gly glycosylated 1104:1115 arg1 the minor human isoform beta-antithrombin the minor human isoform beta-antithrombin PUBTATOR antithrombin 462 The high heparin binding affinity of the salmon inhibitor, Kd of 2.2 and 48 nM at I = 0.15 and 0.3, respectively, is very similar to that of the minor human isoform beta-antithrombin, which is not glycosylated at Asn135. 2498325 12 32 gly glycosylation 1825:1837 arg1 apoE apoE PUBTATOR apoE P02649 The transfected ldlD cells also secreted high levels of apoE even in the absence of glycosylation, which confirms that glycosylation is not essential for secretion of apoE. 3651384 0 61 gly glycoprotein 47:58 arg1 sulfated glycoprotein 2 sulfated glycoprotein 2 PUBTATOR sulfated glycoprotein 2 24854 Biosynthesis and molecular cloning of sulfated glycoprotein 2 secreted by rat Sertoli cells. 17322565 2 42 gly glycosylation 516:528 arg1 EL EL PUBTATOR EL 9388 The current study demonstrates that mutagenesis of either Asn-116 to threonine (Thr) or Thr-118 to Ala also disrupted the glycosylation of EL and enhanced catalytic activity toward synthetic substrates by 3-fold versus wild-type EL. 8486673 5 69 gly contained 698:706 arg1 rat CRP AND one oligosaccharide chain rat CRP one oligosaccharide chain PUBTATOR CRP 25419 FAB-MS, electrospray-mass spectrometry, and linkage analysis demonstrated that each monomer of rat CRP contained one oligosaccharide chain, predominantly a disialylated biantennary structure, attached to Asn-128. 12545205 11 32 gly N-cadherin 1793:1802 arg1 N-glycans N-cadherin N-glycans PUBTATOR N-cadherin 1000 The results demonstrated that N-glycans of N-cadherin are altered in metastatic melanomas in a way characteristic for invasive tumor cells. 1883960 0 91 gly glycosylation 41:53 arg1 human interleukin-6 human interleukin-6 PUBTATOR interleukin-6 3569 Marked cell-type-specific differences in glycosylation of human interleukin-6. 9524075 12 50 gly Non-glycosylated 1303:1318 arg1 Non-glycosylated procathepsin S Non-glycosylated procathepsin S Cterm Non-glycosylated procathepsin S 1520 Non-glycosylated procathepsin S was bound to the plasma membrane at 2 degrees C, suggesting an additional sorting motif in the cathepsin S molecule besides the Man-6-phosphate residue. 21752865 3 59 gly glycosylated 714:725 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Its apparent molecular mass (42-44 kDa) is significantly higher than that predicted by the number and composition of amino acids (30 kDa) suggesting that BRI2 is glycosylated. 15628971 7 83 gly glycosylation 1461:1473 arg1 recombinant C4ST recombinant C4ST PUBTATOR C4ST 314694 In addition, the N-linked oligosaccharide at the C-terminal region appears to affect the glycosylation pattern of recombinant C4ST; a broad protein band of the wildtype protein resulting from microheterogeneity of N-linked oligosaccharides disappeared and four discrete protein bands with different numbers of N-linked oligosaccharides appeared when the N-linked oligosaccharide at the C-terminal region was deleted. 10715549 7 40 gly N-glycosylation 1344:1358 arg1 CGbeta-N13 CGbeta-N13 PUBTATOR SpecificSite CGbeta 1082 Both CGbeta wild-type (WT) and CGbeta lacking N-glycosylation at Asn(13) (CGbeta-N13) showed aggregates in lysate. 10092871 0 54 gly metalloproteinase-1 30:48 arg1 N-glycan structures matrix metalloproteinase-1 N-glycan structures PUBTATOR matrix metalloproteinase-1 4312 N-glycan structures of matrix metalloproteinase-1 derived from human fibroblasts and from HT-1080 fibrosarcoma cells. 24342833 4 17 gly Type-β-catenin 725:738 arg1 potential O-GlcNAc sites -catenin potential O-GlcNAc sites PUBTATOR -catenin 1499 METHOD: Missense mutations were introduced to potential O-GlcNAc sites of pEGFP-C2-N-Terminal- or pEGFP-C2-Wild Type-β-catenin by site-directed mutagenesis. 10894551 4 40 gly glycosylated 532:543 arg1 the ANP receptor the ANP receptor OGER ANP receptor P01160 Here we present the crystal structure of the glycosylated dimerized hormone-binding domain of the ANP receptor at 2.0-A resolution. 26283364 5 15 gly SSL3 785:788 arg1 the conserved Lewis(X) binding site SSL3 the conserved Lewis(X) binding site Cterm SSL3 Q12988 The structure reveals that formation of the specific inhibitory complex is predominantly mediated by hydrophobic contacts between SSL3 and TLR2 and does not involve interaction of TLR2-glycans with the conserved Lewis(X) binding site of SSL3. 3667593 0 43 gly interferon-beta 46:60 arg1 the carbohydrate moiety interferon-beta the carbohydrate moiety PUBTATOR interferon-beta 3456 Structure of the carbohydrate moiety of human interferon-beta secreted by a recombinant Chinese hamster ovary cell line. 8477709 1 9 part_of erythropoietin 199:212 arg1 Ser126 erythropoietin Ser126 PUBTATOR AminoAcid erythropoietin 2056 Ser126 The native structures of the Asn-linked oligosaccharides and the O-glycans at Ser126 of human erythropoietin expressed from recombinant BHK cells have been elucidated. 2498325 11 66 gly sialylated 1689:1698 arg1 multiply sialylated apoE multiply sialylated apoE PUBTATOR apoE 348 The transfected wild-type cells secreted multiply sialylated apoE. 1517205 2 31 gly factor 504:509 arg1 a tetrasaccharide O-fucosidically factor IX a tetrasaccharide O-fucosidically OGER factor IX P00740 We now have evidence of another modification in the first EGF-like domain of human factor IX, which proved to be a tetrasaccharide O-fucosidically linked to Ser-61. 23894062 8 94 part_of NOG 1130:1132 arg1 NOG-N54 NOG NOG-N54 PUBTATOR SpecificSite NOG 9241 N54 We showed a SOST peptide (SOST-S146, with homology to a bacterial glycotransferase peptide) binds to a NOG peptide (NOG-N54), which contains a N-glycosylation site. 16234806 2 5 gly glycoprotein 258:269 arg1 Cbln1 Cbln1 PUBTATOR Cbln1 12404 We show that Cbln1 is a glycoprotein secreted from cerebellar granule cells that is essential for three processes in cerebellar Purkinje cells: the matching and maintenance of pre- and postsynaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses. 28252640 3 68 part_of BoNT/A1 606:612 arg1 Arg 1294 BoNT residues, Arg 1156 and Arg 1294 Cterm SpecificSite BoNT residues, Arg 1156 and Arg 1294 Notably, two BoNT/A1 residues, Arg 1156 and Arg 1294, that are crucial for the interaction with SV2, are not conserved among subtypes. 28252640 3 68 part_of BoNT/A1 606:612 arg1 two BoNT/A1 residues BoNT residues, Arg 1156 and Arg 1294 Cterm SpecificSite BoNT residues, Arg 1156 and Arg 1294 Notably, two BoNT/A1 residues, Arg 1156 and Arg 1294, that are crucial for the interaction with SV2, are not conserved among subtypes. 28252640 3 68 part_of BoNT/A1 606:612 arg1 two BoNT/A1 residues BoNT residues, Arg 1156 and Arg 1294 Cterm SpecificSite BoNT residues, Arg 1156 and Arg 1294 Notably, two BoNT/A1 residues, Arg 1156 and Arg 1294, that are crucial for the interaction with SV2, are not conserved among subtypes. 21932778 5 75 gly glycosylation 732:744 arg1 glypican-1 glypican-1 PUBTATOR glypican-1 2817 Experiments using the drug tunicamycin to inhibit the N-linked glycosylation of glypican-1 showed that secretion of anchorless glypican-1 was reduced and that the protein did not accumulate inside the cells. 23592772 8 48 gly subunit 1200:1206 arg1 O-GlcNAcylated NF-κB p65 subunit O-GlcNAcylated PUBTATOR NF-κB p65 subunit 5970 The NF-κB p65 subunit and upstream kinases IKKα/IKKβ were O-GlcNAcylated in PDAC. 23661698 8 6 part_of EphA2 1196:1200 arg1 Asp-78 EphA2 Asp-78 and Lys-136 PUBTATOR SpecificSite EphA2 1969 Asp-78 and Lys-136 Analysis of Eph/ephrin crystal structures reveals an interaction between the ligand's carbohydrates and two residues of EphA2: Asp-78 and Lys-136. 8670172 9 96 part_of Asn-18 1358:1363 arg1 CD59u CD59u Asn-18 PUBTATOR SpecificSite CD59u 966 Asn-18 The N-linked carbohydrate side chain of CD59u (at Asn-18) also displayed considerable heterogeneity. 2813359 5 17 gly contains 850:857 arg1 EPO-bi AND the biantennary complex type EPO-bi the biantennary complex type PUBTATOR EPO 2056 However, structural analyses of their N-linked sugar chains revealed that EPO-bi contains the biantennary complex type as the major sugar chain, while EPO-tetra and the standard EPO contain the tetraantennary complex type as the major sugar chain. 2813359 5 17 gly contains 850:857 arg1 EPO-bi AND the major sugar chain EPO-bi the major sugar chain PUBTATOR EPO 2056 However, structural analyses of their N-linked sugar chains revealed that EPO-bi contains the biantennary complex type as the major sugar chain, while EPO-tetra and the standard EPO contain the tetraantennary complex type as the major sugar chain. 2813359 5 45 gly contain 951:957 arg1 the standard EPO AND the major sugar chain EPO the major sugar chain PUBTATOR EPO 2056 However, structural analyses of their N-linked sugar chains revealed that EPO-bi contains the biantennary complex type as the major sugar chain, while EPO-tetra and the standard EPO contain the tetraantennary complex type as the major sugar chain. 2813359 5 45 gly contain 951:957 arg1 the standard EPO AND the tetraantennary complex type EPO the tetraantennary complex type PUBTATOR EPO 2056 However, structural analyses of their N-linked sugar chains revealed that EPO-bi contains the biantennary complex type as the major sugar chain, while EPO-tetra and the standard EPO contain the tetraantennary complex type as the major sugar chain. 18214858 1 30 gly fucosylated 213:223 arg1 fucosylated haptoglobin fucosylated haptoglobin PUBTATOR haptoglobin 3240 It was found in our previous studies that the concentration of fucosylated haptoglobin had increased in the sera of patients with pancreatic cancer (PC) compared to those of other types of cancer and normal controls. 8702840 7 25 gly sites 1071:1075 arg1 Thr19 Thr19 Thr19 AminoAcid Thr19 and Ser34 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 25 gly sites 1071:1075 arg1 Ser34 Ser34 Ser34 AminoAcid Thr19 and Ser34 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 25 gly sites 1071:1075 arg1 Ser48 Ser48 Ser48 AminoAcid Ser34 and Ser48 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 18214858 3 37 gly haptoglobin 600:610 arg1 site-specific N-glycan structures haptoglobin site-specific N-glycan structures PUBTATOR haptoglobin 3240 In the present study, site-specific N-glycan structures of haptoglobin in sera obtained from patients with PC or chronic pancreatitis (CP) were analyzed using liquid chromatography-electrospray ionization mass spectrometry. 1904059 9 114 gly observed 1249:1256 arg1 plasma factor VII AND the three glycan structures plasma factor VII the three glycan structures OGER factor VII P08709 Approximately equal amounts of the three glycan structures were observed in plasma factor VII, whereas in recombinant factor VII the glucose and the glucose-(xylose)2 structures predominated. 18642129 5 14 gly O-glycosylation 692:706 arg1 pd-FVII pd-FVII OGER FVII P08709 N- and O-glycosylation sites and site specific heterogeneity of pd-FVII were studied by various complementary qualitative and quantitative techniques. 18642129 5 84 gly heterogeneity 732:744 arg1 pd-FVII pd-FVII OGER FVII P08709 N- and O-glycosylation sites and site specific heterogeneity of pd-FVII were studied by various complementary qualitative and quantitative techniques. 8172892 6 50 gly alpha-D4 993:1000 arg1 Component sugar and sialic acid analyses AP alpha Component sugar and sialic acid analyses PUBTATOR AP alpha 2028 Component sugar and sialic acid analyses of AP alpha-D4 and AP alpha-D5 revealed that they contained 1 mol each of N-acetyl-D-galactosamine (GalNAc), D-galactose (Gal), and sialic acid. 8172892 6 95 gly alpha-D5 1009:1016 arg1 Component sugar and sialic acid analyses AP alpha Component sugar and sialic acid analyses PUBTATOR AP alpha 2028 Component sugar and sialic acid analyses of AP alpha-D4 and AP alpha-D5 revealed that they contained 1 mol each of N-acetyl-D-galactosamine (GalNAc), D-galactose (Gal), and sialic acid. 23187000 3 21 part_of N30 581:583 arg1 TIMP-1 TIMP-1 N30 PUBTATOR SpecificSite TIMP-1 7076 N30 and N78 Among TIMPs, TIMP-1, a 184-residue protein, is the only N-linked glycoprotein with glycosylation sites at N30 and N78. 23187000 3 66 part_of N78 589:591 arg1 TIMP-1 TIMP-1 N78 PUBTATOR SpecificSite TIMP-1 7076 N30 and N78 Among TIMPs, TIMP-1, a 184-residue protein, is the only N-linked glycoprotein with glycosylation sites at N30 and N78. 29932112 6 25 gly N-glycosylation 862:876 arg1 Panx2 Panx2 PUBTATOR Panx2 56666 Our results showed that N86 is the only N-glycosylation site of Panx2. 10842180 0 40 gly N-glycosylated 13:26 arg1 Doppel Doppel PUBTATOR Doppel 26434 Doppel is an N-glycosylated, glycosylphosphatidylinositol-anchored protein. 24417605 10 50 gly sialylated 1518:1527 arg1 HDL HDL OGER HDL Q9UNE0 This report describes for the first time a glycomic approach for analyzing HDL, highlighting that HDL are highly sialylated particles. 26563299 6 2 part_of IL-15 881:885 arg1 IL-15 Asn79 IL-15 IL-15 Asn79 PUBTATOR AminoAcid IL-15 3600 Asn79 IL-15 Asn79 and sIL-15Rα Asn107 carried the same repertoire of biosynthetically-related N-glycans covering mostly α1-6-core-fucosylated and β-GlcNAc-terminating complex-type structures. 26563299 6 93 part_of sIL-15Rα 897:904 arg1 sIL-15Rα Asn107 sIL sIL-15Rα Asn107 OGER AminoAcid sIL Q15468 Asn107 IL-15 Asn79 and sIL-15Rα Asn107 carried the same repertoire of biosynthetically-related N-glycans covering mostly α1-6-core-fucosylated and β-GlcNAc-terminating complex-type structures. 2573604 0 66 gly glycosylation 9:21 arg1 rat renal gamma-glutamyltranspeptidase rat renal gamma-glutamyltranspeptidase PUBTATOR gamma-glutamyltranspeptidase 116568 O-linked glycosylation of rat renal gamma-glutamyltranspeptidase adjacent to its membrane anchor domain. 2033371 2 41 gly CD43 459:462 arg1 O-glycans CD43 O-glycans PUBTATOR CD43 6693 Since the 70 to 80 O-linked carbohydrate chains of CD43 are known to influence markedly its electrophoretic mobility, we analyzed the structure and the biosynthesis of O-glycans of CD43 in lymphocytes from patients with WAS. 2033371 2 77 gly CD43 329:332 arg1 the 70 to 80 O-linked carbohydrate chains CD43 the 70 to 80 O-linked carbohydrate chains PUBTATOR CD43 6693 Since the 70 to 80 O-linked carbohydrate chains of CD43 are known to influence markedly its electrophoretic mobility, we analyzed the structure and the biosynthesis of O-glycans of CD43 in lymphocytes from patients with WAS. 108268 0 31 gly chain 44:48 arg1 the oligosaccharide J chain the oligosaccharide PUBTATOR J chain 3512 Structure of the oligosaccharide of human J chain. 19951703 3 40 gly N-glycosylation 318:332 arg1 human UGT1A9 human UGT1A9 PUBTATOR UGT1A9 54600 In the present study, we investigated the role of N-glycosylation in the function of human UGT1A9. 18533687 10 61 part_of RXFP1 1661:1665 arg1 Asn-303 RXFP1 Asn-303 PUBTATOR SpecificSite RXFP1 59350 Asn-303 In particular, N-glycosylation at Asn-303 of RXFP1 was required for optimal intracellular cAMP signaling. 21689651 0 6 gly glycosylated 58:69 arg1 TMEM235 TMEM235 OGER TMEM235 A6NFC5 The cataract-associated protein TMEM114, and TMEM235, are glycosylated transmembrane proteins that are distinct from claudin family members. 21689651 0 6 gly glycosylated 58:69 arg1 The cataract-associated protein TMEM114 The cataract-associated protein TMEM114 PUBTATOR TMEM114 B3SHH9 The cataract-associated protein TMEM114, and TMEM235, are glycosylated transmembrane proteins that are distinct from claudin family members. 9767079 1 45 gly N-glycosylation 153:167 arg1 folate carrier folate carrier PUBTATOR reduced folate carrier 6573 The role of N-glycosylation in reduced folate carrier (RFC) transport and membrane targeting was examined in transport-deficient K562 (K500E) cells transfected with human RFC cDNAs. 9767079 1 45 gly N-glycosylation 153:167 arg1 RFC RFC PUBTATOR RFC 6573 The role of N-glycosylation in reduced folate carrier (RFC) transport and membrane targeting was examined in transport-deficient K562 (K500E) cells transfected with human RFC cDNAs. 23010571 5 10 gly glycoprotein 762:773 arg1 Recombinant ADAMTSL5 Recombinant ADAMTSL5 PUBTATOR Recombinant ADAMTSL5 339366 Recombinant ADAMTSL5 is a secreted, N-glycosylated 60kDa glycoprotein located in the subcellular matrix, on the cell-surface, and in the medium of transfected cells. 28279966 0 14 part_of K1491-R1492 41:51 arg1 Von Willebrand Factor Plasmin Cleaves Von Willebrand Factor K1491-R1492 PUBTATOR SiteSequence Plasmin Cleaves Von Willebrand Factor 5340 K1491-R1492 Plasmin Cleaves Von Willebrand Factor at K1491-R1492 in the A1-A2 Linker Region in a Shear- and Glycan-Dependent Manner In Vitro. 26271046 4 8 gly GluA2 817:821 arg1 N-glycans GluA2 N-glycans PUBTATOR GluA2 2891 However, evidence that the HNK-1 epitope on N-glycans of GluA2 directly affects these phenomena is lacking. 16763549 0 13 gly neuropilin-1 34:45 arg1 Glycosaminoglycan modification neuropilin-1 Glycosaminoglycan modification OGER neuropilin-1 O14786 Glycosaminoglycan modification of neuropilin-1 modulates VEGFR2 signaling. 16763549 0 31 gly modification 18:29 arg1 neuropilin-1 AND Glycosaminoglycan modification neuropilin-1 Glycosaminoglycan modification OGER neuropilin-1 O14786 Glycosaminoglycan modification of neuropilin-1 modulates VEGFR2 signaling. 29932112 1 1 gly glycoproteins 147:159 arg1 Pannexins Pannexins PUBTATOR Pannexins (Panx1 24145 Pannexins (Panx1, 2, 3) are channel-forming glycoproteins expressed in mammalian tissues. 19690161 7 38 gly glycosylation 1036:1048 arg1 vIL-6 vIL-6 OGER vIL P09327 With the use of a conformation-specific antibody and tryptic digestion assays, we showed that glycosylation at the Asn-89 site of vIL-6 affected protein conformation. 20959806 5 6 gly Snail1 847:852 arg1 O-GlcNAc modification Snail1 O-GlcNAc modification PUBTATOR Snail1 6615 Thus, dynamic reciprocal O-phosphorylation and O-GlcNAc modification of Snail1 constitute a molecular link between cellular glucose metabolism and the control of EMT. 20959806 5 14 gly EMT 937:939 arg1 cellular glucose metabolism EMT cellular glucose metabolism OGER EMT Q08881 Thus, dynamic reciprocal O-phosphorylation and O-GlcNAc modification of Snail1 constitute a molecular link between cellular glucose metabolism and the control of EMT. 20959806 5 15 gly modification 831:842 arg3 Snail1 AND O-GlcNAc modification Snail1 O-GlcNAc modification PUBTATOR Snail1 6615 Thus, dynamic reciprocal O-phosphorylation and O-GlcNAc modification of Snail1 constitute a molecular link between cellular glucose metabolism and the control of EMT. 10531415 0 46 gly glycosylation 9:21 arg1 D1 D1 Cterm D1 N-linked glycosylation is required for plasma membrane localization of D5, but not D1, dopamine receptors in transfected mammalian cells. 23527852 1 10 gly glycoprotein 175:186 arg1 Apolipoprotein-CIII Apolipoprotein-CIII PUBTATOR Apolipoprotein-CIII 345 Apolipoprotein-CIII (apoCIII) is an abundant blood glycoprotein associated with lipoprotein particles. 15616124 2 83 gly N-glycosylation 312:326 arg1 FVII FVII Cterm FVII 2155 In the present study, however, we demonstrate posttranslational N-glycosylation of recombinant human coagulation factor VII (FVII) in CHO-K1 and 293A cells. 15616124 2 83 gly N-glycosylation 312:326 arg1 recombinant human coagulation factor VII recombinant human coagulation factor VII PUBTATOR coagulation factor VII 2155 In the present study, however, we demonstrate posttranslational N-glycosylation of recombinant human coagulation factor VII (FVII) in CHO-K1 and 293A cells. 19413349 7 36 gly deglycosylation 1213:1227 arg1 wild-type or mutant melanopsin wild-type or mutant melanopsin PUBTATOR melanopsin 192223 Further in vitro O-linked deglycosylation of wild-type or mutant melanopsin with O-glycosidase and neuraminidase converted the 55 kDa band to a 49 kDa band. 21053369 4 75 gly glycosylation 419:431 arg1 rhIL-23r rhIL-23r OGER rhIL-23r Q5VWK5 In this work, glycosylation profiles of soluble recombinant human IL-23r (rhIL-23r) were established using mass spectrometry (MS), which included defining glycosylation sites, degree of glycosylation occupancy of each site and structure of attached oligosaccharides. 21053369 4 75 gly glycosylation 419:431 arg1 soluble recombinant human IL-23r soluble recombinant human IL-23r PUBTATOR IL-23r 149233 In this work, glycosylation profiles of soluble recombinant human IL-23r (rhIL-23r) were established using mass spectrometry (MS), which included defining glycosylation sites, degree of glycosylation occupancy of each site and structure of attached oligosaccharides. 25327667 0 54 gly glycosylation 33:45 arg1 prostate specific antigen prostate specific antigen PUBTATOR prostate specific antigen 354 Glycoproteomics: identifying the glycosylation of prostate specific antigen at normal and high isoelectric points by LC-MS/MS. 1544894 7 69 gly has 1145:1147 arg1 human factor VII AND O-linked fucose human factor VII O-linked fucose OGER factor VII P08709 It has been recently reported that the first EGF domain of human factor VII has O-linked fucose at the equivalent position (Ser-60) (Bjoern, S., Foster, D. C., Thim, L., Wiberg, F. C., Christensen, M., Komiyama, Y., Pedersen, A. H., and Kisiel, W. (1991) J. Biol. 24417605 5 59 gly glycoproteins 648:660 arg1 the N-glycans HDL glycoproteins the N-glycans OGER HDL glycoproteins Q9UNE0 Most of the N-glycans (∼90%) from HDL glycoproteins were sialylated with one or two neuraminic acids (Neu5Ac). 28784760 4 12 part_of NPC1 642:645 arg1 residues 314-1,278 NPC1 residues 314-1,278 PUBTATOR SpecificSite NPC1 4864 residues 314 Here, we report a crystal structure at 3.3 Å resolution of NPC1* (residues 314-1,278), which-in contrast to previous lower resolution structures-features the entire CTD well resolved. 17715132 3 18 gly N-glycosylated 480:493 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We show here that Pannexin1 forms a hexameric channel and reaches the cell surface but, unlike connexins, is N-glycosylated. 20406422 2 27 part_of N371 453:456 arg1 CLN7 CLN7 N371 PUBTATOR SpecificSite CLN7 256471 N371 and N376 In this study fluorescence protease protection assays and mutational analyses revealed the N- and C-terminal tails of CLN7 in the cytosol and two N-glycosylation sites at N371 and N376. 20406422 2 30 part_of N376 462:465 arg1 CLN7 CLN7 N376 PUBTATOR SpecificSite CLN7 256471 N371 and N376 In this study fluorescence protease protection assays and mutational analyses revealed the N- and C-terminal tails of CLN7 in the cytosol and two N-glycosylation sites at N371 and N376. 3264725 8 62 gly glycosylated 1584:1595 arg1 the recombinant factor VIIa the recombinant factor VIIa Cterm factor VIIa In the recombinant factor VIIa, asparagine residue 322 was fully glycosylated whereas asparagine residue 145 was only partially (approximately 66%) glycosylated. 8130392 9 68 gly oligosaccharides 1429:1444 arg1 rHPC are rHPC oligosaccharides OGER rHPC P52873 The structure of the major neutral oligosaccharide in rHPC was determined to be: [formula: see text] Two representatives of the sialylated oligosaccharides in rHPC are: [formula: see text] and [formula: see text] Thus, many of the Asn-linked oligosaccharides in rHPC were found to terminate in GalNAc beta (1-->4)GlcNAc beta (1-->.) 8130392 9 86 gly oligosaccharides 1532:1547 arg1 rHPC rHPC oligosaccharides OGER rHPC P52873 The structure of the major neutral oligosaccharide in rHPC was determined to be: [formula: see text] Two representatives of the sialylated oligosaccharides in rHPC are: [formula: see text] and [formula: see text] Thus, many of the Asn-linked oligosaccharides in rHPC were found to terminate in GalNAc beta (1-->4)GlcNAc beta (1-->.) 8130392 9 128 gly oligosaccharide 1325:1339 arg1 rHPC rHPC oligosaccharide OGER rHPC P52873 The structure of the major neutral oligosaccharide in rHPC was determined to be: [formula: see text] Two representatives of the sialylated oligosaccharides in rHPC are: [formula: see text] and [formula: see text] Thus, many of the Asn-linked oligosaccharides in rHPC were found to terminate in GalNAc beta (1-->4)GlcNAc beta (1-->.) 7916636 4 39 part_of contain 471:477 arg1 urinary kallikrein AND a Lys-162 urinary kallikrein a Lys-162 PUBTATOR SpecificSite kallikrein 9622 Lys-162 The human kallikrein gene and urinary kallikrein both contain a Lys-162 instead of the reported Glu-162. 16622833 5 66 gly acids 733:737 arg1 glycosylation site N107 glycosylation site N107 glycosylation site N107 SpecificSite site N107 The variability of the number of antennae and hence sialic acids on glycosylation site N107, which even contained minute amounts of tetraantennary structures, emerged as a major cause for the IEF pattern of A1PI. 16622833 5 74 gly antennae 707:714 arg1 glycosylation site N107 glycosylation site N107 glycosylation site N107 SpecificSite site N107 The variability of the number of antennae and hence sialic acids on glycosylation site N107, which even contained minute amounts of tetraantennary structures, emerged as a major cause for the IEF pattern of A1PI. 15869464 3 61 gly glycosylation 664:676 arg1 native human OPN native human OPN PUBTATOR OPN 6696 Using a combination of Edman degradation and MS analyses, we have characterized the complete phosphorylation and glycosylation pattern of native human OPN. 2108149 2 85 gly glycosylation 401:413 arg1 the IgGs the IgGs Cterm IgGs The glycosylation patterns of the IgGs of the parental and fused cells were studied. 21327254 6 9 gly Tau 1162:1164 arg1 the microtubule-associated repeats Tau the microtubule-associated repeats Cterm Tau Here, we identify three O-GlcNAc sites by screening a library of small peptides sampling the proline-rich, the microtubule-associated repeats and the carboxy-terminal domains of Tau as potential substrates for the O-β-N-acetylglucosaminyltransferase (OGT). 1577715 0 32 gly factor 83:88 arg1 the asparagine-linked oligosaccharide chains von Willebrand factor the asparagine-linked oligosaccharide chains PUBTATOR von Willebrand factor 7450 Structures of the asparagine-linked oligosaccharide chains of human von Willebrand factor. 24884609 6 1 gly N-glycoforms 998:1009 arg1 ITIH4 N-glycoforms ITIH4 N-glycoforms PUBTATOR ITIH4 3700 Next, we performed glycosidase-assisted LC-MS/MS analysis of ITIH4 trypsin-GluC glycopeptides enriched via hydrophilic interaction liquid chromatography to characterize ITIH4 N-glycoforms. 6684483 0 2 gly globulin 68:75 arg1 the carbohydrate moiety sex hormone-binding globulin the carbohydrate moiety PUBTATOR sex hormone-binding globulin 6462 Study of the carbohydrate moiety of human serum sex hormone-binding globulin. 18775496 0 27 gly agrin 25:29 arg1 O-fucosylation agrin O-fucosylation PUBTATOR agrin O00468 O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. 18775496 0 38 gly O-fucosylation 0:13 arg1 muscle agrin muscle agrin PUBTATOR agrin O00468 O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. 14718370 0 31 gly MUC5AC 19:24 arg1 C-Mannosylation MUC5AC C-Mannosylation PUBTATOR MUC5AC P98088 C-Mannosylation of MUC5AC and MUC5B Cys subdomains. 14718370 0 35 gly MUC5B 30:34 arg1 C-Mannosylation MUC5B C-Mannosylation PUBTATOR MUC5B Q9HC84 C-Mannosylation of MUC5AC and MUC5B Cys subdomains. 22750213 6 56 gly glycosylation 877:889 arg1 mouse PRiMA mouse PRiMA PUBTATOR PRiMA 170952 Abolishing glycosylation on mouse PRiMA appeared not to affect its assembly with AChE(T), the enzymatic properties of AChE, and the membrane trafficking of PRiMA-linked AChE tetramers. 17082223 1 67 gly glycosylation 185:197 arg1 the bile salt export pump [Bsep the bile salt export pump [Bsep OGER bile salt export pump O70127 The aim of this study was to determine the role of N-linked glycosylation in protein stability, intracellular trafficking, and bile acid transport activity of the bile salt export pump [Bsep (ATP-binding cassette B11)]. 9425062 8 11 gly oligosaccharides 1085:1100 arg1 Thr637 Thr637 Thr637 AminoAcid Thr612, Ser619, Thr621, and Thr637 In addition, the IalphaI heavy chains carried several O-linked glycans located on Thr619 of heavy chain 1 and a cluster of four O-linked oligosaccharides on Thr612, Ser619, Thr621, and Thr637 of heavy chain 2. 9425062 8 11 gly oligosaccharides 1085:1100 arg1 Ser619 Ser619 Ser619 AminoAcid Thr612, Ser619, Thr621, and Thr637 In addition, the IalphaI heavy chains carried several O-linked glycans located on Thr619 of heavy chain 1 and a cluster of four O-linked oligosaccharides on Thr612, Ser619, Thr621, and Thr637 of heavy chain 2. 9425062 8 11 gly oligosaccharides 1085:1100 arg1 Thr612 Thr612 Thr612 AminoAcid Thr612, Ser619, Thr621, and Thr637 In addition, the IalphaI heavy chains carried several O-linked glycans located on Thr619 of heavy chain 1 and a cluster of four O-linked oligosaccharides on Thr612, Ser619, Thr621, and Thr637 of heavy chain 2. 9425062 8 11 gly oligosaccharides 1085:1100 arg1 Thr621 Thr621 Thr621 AminoAcid Thr612, Ser619, Thr621, and Thr637 In addition, the IalphaI heavy chains carried several O-linked glycans located on Thr619 of heavy chain 1 and a cluster of four O-linked oligosaccharides on Thr612, Ser619, Thr621, and Thr637 of heavy chain 2. 11439087 8 49 part_of sBST-1 1133:1138 arg1 N1-N4 sBST-1 N1-N4 Cterm SiteSequence sBST-1 683 N1-N4 Site-directed mutagenesis was performed to generate sBST-1 mutants (N1-N4), each preserving a single N-glycosylation site. 21264968 5 95 gly MUC5AC 1826:1831 arg1 four α-GalNAc residues MUC5AC four α-GalNAc residues PUBTATOR MUC5AC 4586 On the contrary, O-glycosylation of naked MUC5AC peptide occurred predominantly at consecutive Thr residues and led to MUC5AC with four α-GalNAc residues at Thr2, Thr3, Thr7, and Thr8. 9757569 0 10 part_of residue 32:38 arg1 ovine angiotensinogen angiotensinogen residue PUBTATOR AminoAcid angiotensinogen 183 residue at position 14 Effects of glycosylation of the residue at position 14 in ovine angiotensinogen on the human renin reaction. 14970177 0 42 gly glycosylation 9:21 arg1 Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6 Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6 OGER interleukin 6 P05231 N-linked glycosylation is required for optimal function of Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6. 8942648 0 85 gly mapping 38:44 arg1 recombinant human thrombopoietin recombinant human thrombopoietin PUBTATOR thrombopoietin 7066 Peptide, disulfide, and glycosylation mapping of recombinant human thrombopoietin from ser1 to Arg246. 9884403 6 39 gly glycoprotein 1322:1333 arg1 native human Tamm-Horsfall glycoprotein native human Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 Profiling of the carbohydrate moieties of Asn208 indicates a large heterogeneity, similar to that established for native human Tamm-Horsfall glycoprotein, namely, multiply charged complex-type carbohydrate structures, terminated by sulfate groups, sialic acid residues, and/or the Sda-determinant. 11562499 3 33 gly glycosylated 539:550 arg1 glycosylated human PEDF glycosylated human PEDF PUBTATOR PEDF 5176 To provide a structural basis for understanding its many biological roles, we have solved the crystal structure of glycosylated human PEDF to 2.85 A. 24884609 3 41 gly glycosylation 443:455 arg1 recombinant and serum-derived ITIH4 recombinant and serum-derived ITIH4 PUBTATOR ITIH4 3700 In this study, we aimed to characterize glycosylation of recombinant and serum-derived ITIH4 using analytical mass spectrometry. 9118998 5 38 gly glycosylations 933:946 arg1 mFA1 mFA1 PUBTATOR mFA1 13386 Three N-linked glycosylations were localized in mFA1 (Asn77, Asn142 and Asn151), two of which (Asn142 and Asn151) were in the unusual Asn-Xaa-Cys motif. 8486673 1 56 gly glycoprotein 131:142 arg1 Rat C-reactive protein Rat C-reactive protein PUBTATOR Rat C-reactive protein 25419 Rat C-reactive protein (CRP) is a pentameric glycoprotein composed of five apparently identical monomers, two of which form a disulfide-linked dimer (Rasosouli, M., Sambasivam, H., Azadi, P., Dell, A., Morris, H. R., Nagpurkar, A., Mookerjea, S., and Murray, R. K. (1992) J. Biol. 27314333 6 43 part_of N137 1080:1083 arg1 Rspo1 Rspo1 N137 PUBTATOR SpecificSite Rspo1 284654 N137 Introduction of the N-glycosylation site to Rspo2 mutant at the position homologous to N137 in Rspo1 restored full glycosylation and rescued the accumulation defect of nonglycosylated Rspo2 mutant in media. 9572875 2 32 gly nonglycosylated 386:400 arg1 Glycosylated and nonglycosylated recombinant human IGFBP-6 Glycosylated and nonglycosylated recombinant human IGFBP-6 PUBTATOR IGFBP-6 3489 Glycosylated and nonglycosylated recombinant human IGFBP-6, expressed in Chinese hamster ovary cells and Escherichia coli, respectively, were purified using IGF-II affinity chromatography and reverse-phase medium-pressure chromatography. 17591618 3 44 gly glycosylation 437:449 arg1 CFH CFH PUBTATOR CFH P08603 In the current study, we present a quantitative glycosylation analysis of CFH using capillary electrophoresis and a complete site-specific N-glycan characterization using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESIMS/MS). 17591618 3 53 gly CFH 463:465 arg1 a complete site-specific N-glycan characterization CFH a complete site-specific N-glycan characterization PUBTATOR CFH P08603 In the current study, we present a quantitative glycosylation analysis of CFH using capillary electrophoresis and a complete site-specific N-glycan characterization using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESIMS/MS). 28207759 4 33 gly PLC 774:776 arg1 9 Shire PLC 9 PUBTATOR Shire PLC 3339 Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans. 28207759 4 33 gly PLC 774:776 arg1 velaglucerase (Shire PLC) Man Shire PLC velaglucerase (Shire PLC) Man PUBTATOR Shire PLC 3339 Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans. 28207759 4 93 gly Shire 768:772 arg1 9 Shire PLC 9 PUBTATOR Shire PLC 3339 Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans. 28207759 4 93 gly Shire 768:772 arg1 velaglucerase (Shire PLC) Man Shire PLC velaglucerase (Shire PLC) Man PUBTATOR Shire PLC 3339 Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans. 28207759 4 53 gly contains 710:717 arg1 imiglucerase AND Man(3) glycans imiglucerase Man(3) glycans PUBTATOR GD: imiglucerase 2629 Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans. 1731338 5 52 gly galactoglycoprotein 897:915 arg1 galactoglycoprotein molecules galactoglycoprotein molecules PUBTATOR galactoglycoprotein 6693 C-terminal analysis revealed multiple C-terminal residues, suggesting that galactoglycoprotein molecules are of varying lengths. 26013384 0 10 part_of Ser126 59:64 arg1 Human Recombinant Erythropoietin Erythropoietin Ser126 PUBTATOR AminoAcid Erythropoietin 2056 Ser126 Structural Identification of a Non-Glycosylated Variant at Ser126 for O-Glycosylation Site from EPO BRP, Human Recombinant Erythropoietin by LC/MS Analysis. 28089369 1 9 gly Notch 300:304 arg1 epidermal growth factor-like (EGF) repeats Notch epidermal growth factor-like (EGF) repeats PUBTATOR Notch 31293 Fringe proteins are β3-N-acetylglucosaminyltransferases that modulate Notch activity by modifying O-fucose residues on epidermal growth factor-like (EGF) repeats of Notch. 11588155 5 12 gly apoB100 982:988 arg1 The N-glycan composition apoB100 The N-glycan composition PUBTATOR apoB100 338 The N-glycan composition of apoB100 derived from five LDL subpopulations (LDL1, d = 1.018-1.023; LDL2, d = 1.023-1.030; LDL3, d = 1.030-1.040; LDL4, d = 1.040-1.051; LDL5, d = 1.051-1.065 g/ml) did not vary in normolipidemic or hypercholesterolemic subjects. 2049076 14 51 gly O-glycosylated 2248:2261 arg1 the only O-glycosylated human IFN-alpha protein the only O-glycosylated human IFN-alpha protein PUBTATOR IFN-alpha protein 3440 As IFN-alpha 2 is the only IFN-alpha species with a threonine residue at position 106, it may represent the only O-glycosylated human IFN-alpha protein. 7505568 2 26 gly glycosylated 217:228 arg1 MAG MAG PUBTATOR MAG 4099 MAG is heavily glycosylated containing 30% carbohydrate by weight. 7309709 1 17 gly contains 93:100 arg1 Human prothrombin AND three asparagine-linked sugar chains Human prothrombin three asparagine-linked sugar chains PUBTATOR Human prothrombin 2147 Human prothrombin contains three asparagine-linked sugar chains in one molecule. 21550978 4 80 gly glycosylation 762:774 arg1 hPAR(1) hPAR(1) PUBTATOR hPAR(1) 2149 We have analyzed the role of N-linked glycosylation in regulating proteinase activation/disarming and cell global expression of hPAR(1). 16014566 6 89 gly glycosylation 1277:1289 arg1 human GPVI human GPVI PUBTATOR GPVI 51206 These findings indicate that N-linked glycosylation at N92 in human GPVI is not required for surface expression, but contributes to maximal adhesion to type I collagen, CRP and, to a lesser extent, CVX. 18988733 4 0 part_of p65 721:723 arg1 Thr-322 NFkappaB p65 Thr-322 and Thr-352 PUBTATOR SpecificSite NFkappaB p65 4790 Thr-322 and Thr-352 Also, we demonstrate that both Thr-322 and Thr-352 of NFkappaB p65 can be modified with O-GlcNAc, but modification on Thr-352, not Thr-322, is important for transcriptional activation. 23376777 4 69 gly glycosylation 711:723 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 We showed that triple (N312/331/344/Q) and quadruple (N312/331/344/360/Q) mutations of N-linked glycosylation sites disrupt the N-linked glycosylation of KCC4, resulting in the accumulation of KCC4, predominantly in the endoplasmic reticulum (ER) and not at the cell surface. 16847056 2 63 gly linked 258:263 arg1 beta1 AND sialic acid residues beta1 sialic acid residues Cterm beta1 We have shown that sialic acid residues linked to Nav alpha and beta1 subunits alter channel gating. 8323299 1 53 gly glycosylated 198:209 arg1 lamp-2 lamp-2 PUBTATOR lamp-2 3920 The lysosomal membrane glycoproteins lamp-1 and lamp-2 are extensively glycosylated with a variety of different carbohydrate structures of both N-linked and O-linked type. 8323299 1 53 gly glycosylated 198:209 arg1 lamp-1 lamp-1 PUBTATOR lamp-1 3916 The lysosomal membrane glycoproteins lamp-1 and lamp-2 are extensively glycosylated with a variety of different carbohydrate structures of both N-linked and O-linked type. 8323299 1 69 gly glycoproteins 150:162 arg1 lamp-2 lamp-2 PUBTATOR lamp-2 3920 The lysosomal membrane glycoproteins lamp-1 and lamp-2 are extensively glycosylated with a variety of different carbohydrate structures of both N-linked and O-linked type. 8323299 1 69 gly glycoproteins 150:162 arg1 lamp-1 lamp-1 PUBTATOR lamp-1 3916 The lysosomal membrane glycoproteins lamp-1 and lamp-2 are extensively glycosylated with a variety of different carbohydrate structures of both N-linked and O-linked type. 15657036 5 50 gly type 603:606 arg1 secreted FGE FGE type PUBTATOR FGE 285362 Intracellular FGE contains a high mannose type N-glycan, which is processed to the complex type in secreted FGE. 15657036 5 14 gly contains 530:537 arg1 Intracellular FGE AND a high mannose type N-glycan Intracellular FGE a high mannose type N-glycan PUBTATOR FGE 285362 Intracellular FGE contains a high mannose type N-glycan, which is processed to the complex type in secreted FGE. 7462199 1 57 gly globulin 147:154 arg1 The asparagine-linked sugar chains cold-insoluble globulin The asparagine-linked sugar chains PUBTATOR cold-insoluble globulin 2335 The asparagine-linked sugar chains of cold-insoluble globulin isolated from human plasma were released as oligosaccharides from the polypeptide moiety by hydrazinolysis. 7462199 1 57 gly globulin 147:154 arg1 oligosaccharides cold-insoluble globulin oligosaccharides PUBTATOR cold-insoluble globulin 2335 The asparagine-linked sugar chains of cold-insoluble globulin isolated from human plasma were released as oligosaccharides from the polypeptide moiety by hydrazinolysis. 9334252 7 19 part_of RNase 1156:1160 arg1 Trp-7 RNase 2 Trp-7 PUBTATOR SpecificSite RNase 2 6036 Trp-7 The observation that pig kidney cells contain the machinery for C-mannosylation of Trp-7 of human RNase 2 but that the homologous RNase from porcine kidney is not a substrate, since it does not contain a tryptophan at position 7, strongly suggests that C-mannosylated proteins other than RNase 2 exist. 20427278 5 15 gly O-glycosylation 678:692 arg1 APP APP OGER APP P05067 TMEM59 transfection inhibited complex N- and O-glycosylation of APP in cultured cells. 27175940 2 14 part_of p27 474:476 arg1 Thr157 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser2 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser106 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser2 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser106 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser106 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 21573946 1 14 gly N-glycosylated 229:242 arg1 EC-SOD EC-SOD PUBTATOR EC-SOD 20657 Extracellular superoxide dismutase (EC-SOD), the major SOD isoenzyme in biological fluids, is known to be N-glycosylated and heterogeneous as was detected in most glycoproteins. 21573946 1 14 gly N-glycosylated 229:242 arg1 Extracellular superoxide dismutase Extracellular superoxide dismutase PUBTATOR Extracellular superoxide dismutase 20657 Extracellular superoxide dismutase (EC-SOD), the major SOD isoenzyme in biological fluids, is known to be N-glycosylated and heterogeneous as was detected in most glycoproteins. 21573946 1 14 gly N-glycosylated 229:242 arg1 the major SOD isoenzyme the major SOD isoenzyme PUBTATOR SOD isoenzyme 6649 Extracellular superoxide dismutase (EC-SOD), the major SOD isoenzyme in biological fluids, is known to be N-glycosylated and heterogeneous as was detected in most glycoproteins. 18533687 8 9 gly N-glycosylation 1305:1319 arg1 RXFP1 RXFP1 PUBTATOR RXFP1 59350 All of the potential N-glycosylation sites of RXFP1 were utilized in HEK-293T cells, and importantly, disruption of glycosylation at individual or combinations of double and triple sites had little effect on relaxin binding. 10889209 0 43 gly Glycosylation 0:12 arg1 GIRK1 GIRK1 PUBTATOR GIRK1 3760 Glycosylation of GIRK1 at Asn119 and ROMK1 at Asn117 has different consequences in potassium channel function. 18642129 12 16 part_of position 1702:1709 arg1 the light chain chain position OGER SpecificSite chain P08709 position Ser(60) and Ser(52) Last, MS and MS/MS analysis revealed that FVII is O-glycosylated on the light chain at position Ser(60) and Ser(52) which are modified by oligosaccharide structures such as fucose and Glc(Xyl)(0-1-2), respectively. 7642555 6 17 gly s 607:607 arg1 c-Myc c-Myc s PUBTATOR c-Myc 4609 In this paper, we identified the O-GlcNAc attachment site(s) on c-Myc. 17650508 1 14 gly glycoprotein 222:233 arg1 myocilin myocilin PUBTATOR myocilin 4653 MYOC, a gene involved in different types of glaucoma, encodes myocilin, a secreted glycoprotein of unknown function, consisting of an N-terminal leucine-zipper-like domain, a central linker region, and a C-terminal olfactomedin-like domain. 9574531 0 43 gly glycosylation 6:18 arg1 HLA-DRalpha HLA-DRalpha OGER HLA Novel glycosylation of HLA-DRalpha disrupts antigen presentation without altering endosomal localization. 9336835 5 19 gly contains 874:881 arg1 the beta subunit AND 27-30% carbohydrate the beta subunit 27-30% carbohydrate OGER subunit 1191 The data indicate that clusterin contains 17-27% carbohydrate by weight, the alpha subunit contains 0-30% carbohydrate and the beta subunit contains 27-30% carbohydrate. 9336835 5 90 gly contains 825:832 arg1 the alpha subunit AND 0-30% carbohydrate the alpha subunit 0-30% carbohydrate OGER subunit 1191 The data indicate that clusterin contains 17-27% carbohydrate by weight, the alpha subunit contains 0-30% carbohydrate and the beta subunit contains 27-30% carbohydrate. 9336835 5 51 gly contains 767:774 arg1 clusterin AND 17-27% carbohydrate clusterin 17-27% carbohydrate PUBTATOR clusterin 1191 The data indicate that clusterin contains 17-27% carbohydrate by weight, the alpha subunit contains 0-30% carbohydrate and the beta subunit contains 27-30% carbohydrate. 3935432 0 67 gly interleukin-2 55:67 arg1 the major carbohydrates interleukin-2 the major carbohydrates PUBTATOR interleukin-2 3558 Structures of the major carbohydrates of natural human interleukin-2. 20378933 9 69 gly desialylation 1394:1406 arg1 the increased IgA binding the increased IgA binding OGER IgA P11912 Furthermore, increased IgA binding was also observed on desialylated FcalphaR after neuraminidase treatment and desialylation of N58 contributed most to the increased IgA binding. 6587378 0 1 gly 2-glycoprotein 50:63 arg1 human plasma beta 2-glycoprotein I human plasma beta 2-glycoprotein I OGER beta 2-glycoprotein I P02749 Complete amino acid sequence of human plasma beta 2-glycoprotein I. 24085305 11 61 gly glycosylated 1774:1785 arg1 optimally glycosylated LILRA3 optimally glycosylated LILRA3 PUBTATOR LILRA3 11026 Binding to monocytes was partially blocked by β-lactose, indicating that optimally glycosylated LILRA3 might be critical for ligand binding and function. 16474139 12 31 gly glycosylated 2100:2111 arg1 the SARS-CoV M protein the SARS-CoV M protein OGER M protein P54296 Thus, the SARS-CoV 3a protein is an O-glycosylated glycoprotein, like the group 2 coronavirus M proteins but unlike the SARS-CoV M protein, which is N glycosylated. 1820200 3 15 gly N-glycosylation 1816:1830 arg1 hCG hCG PUBTATOR hCG 93659 The distinct site-specific distribution of the oligosaccharide structures among individual N-glycosylation sites of hCG appears to reflect primarily the influence of the surrounding protein structure on the substrate accessibility of the Golgi processing enzymes alpha-mannosidase II, GlcNAc transferase II and alpha 1,6-fucosyltransferase. 22159084 7 19 gly Deglycosylation 821:835 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 Deglycosylation of SLC26A3 causes depression of transport activity compared with wild-type, although robust intracellular pH changes were still observed, suggesting that N-glycosylation is not absolutely necessary for transport activity. 8349699 3 99 gly Glycosylation 349:361 arg1 PGH synthase-1 PGH synthase-1 PUBTATOR PGH synthase-1 19224 Glycosylation of PGH synthase-1 at Asn410 and at either Asn68 or Asn144 was required for expression of both the cyclooxygenase and the peroxidase activities of the enzyme. 2793860 8 18 gly O-glycosylated 1279:1292 arg1 O-glycosylated human IL-2 O-glycosylated human IL-2 PUBTATOR IL-2 P60568 Our results show that O-glycosylated human IL-2 can be produced by applying recombinant DNA technology in heterologous cell lines with the same type of post-translational modification that is observed for the protein secreted from natural T lymphocytes. 17139081 0 41 gly glycosylated 37:48 arg1 acid-beta-glucosidase acid-beta-glucosidase PUBTATOR acid-beta-glucosidase 2629 Structural comparison of differently glycosylated forms of acid-beta-glucosidase, the defective enzyme in Gaucher disease. 19556306 0 61 part_of receptor 44:51 arg1 Cys(88) Toll-like receptor 4 Cys(88) PUBTATOR SpecificSite Toll-like receptor 4 7099 Cys(88) Mutational analysis of Cys(88) of Toll-like receptor 4 highlights the critical role of MD-2 in cell surface receptor expression. 19690161 7 29 part_of vIL-6 1072:1076 arg1 the Asn-89 site vIL the Asn-89 site OGER SpecificSite vIL P09327 Asn-89 site With the use of a conformation-specific antibody and tryptic digestion assays, we showed that glycosylation at the Asn-89 site of vIL-6 affected protein conformation. 2001369 3 58 gly rCD4 662:665 arg1 the Asn-linked oligosaccharides rCD4 the Asn-linked oligosaccharides PUBTATOR rCD4 24932 In the present study, the structures of the Asn-linked oligosaccharides of soluble rCD4 have been elucidated. 7068558 1 13 gly contains 144:151 arg1 Human lactoferrin AND 2 asparagine-linked sugar chains Human lactoferrin 2 asparagine-linked sugar chains OGER Human lactoferrin P02788 Human lactoferrin contains 2 asparagine-linked sugar chains in 1 molecule. 21768335 4 23 gly glycosylated 676:687 arg1 a glycosylated Fcγ receptor a glycosylated Fcγ receptor Cterm Fcγ In this study, the crystal structures of a glycosylated Fcγ receptor complexed with either afucosylated or fucosylated Fc were determined allowing a detailed, molecular understanding of the regulatory role of Fc-oligosaccharide core fucosylation in improving ADCC. 18420577 4 13 gly O-GlcNAc 687:694 arg1 hepatic FoxO1 FoxO1 O-GlcNAc PUBTATOR FoxO1 2308 Here we show that O-GlcNAc on hepatic FoxO1 is increased in diabetes. 3571235 4 18 part_of B 536:536 arg1 residues 36-71 glycophorin B residues 36-71 PUBTATOR SpecificSite glycophorin B 2994 residues 36-71 The amino acid sequence of the intramembranous domain (residues 36-71) of glycophorin B was determined and found to be similar to that of the hydrophobic region of the major sialoglycoprotein (glycophorin A). 10731668 2 32 gly contains 370:377 arg1 the CHO sEGFR AND one oligosaccharide chain the CHO sEGFR one oligosaccharide chain PUBTATOR CHO sEGFR P00533 We have found that the CHO sEGFR contains one oligosaccharide chain attached to an atypical N-glycosylation consensus sequence, Asn(32 )-X( 33 )-Cys(34 ). 21752865 0 42 gly Glycosylation 0:12 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Glycosylation of BRI2 on asparagine 170 is involved in its trafficking to the cell surface but not in its processing by furin or ADAM10. 8130392 7 58 gly rHPC 1017:1020 arg1 The Asn-linked oligosaccharides rHPC The Asn-linked oligosaccharides OGER rHPC P52873 The Asn-linked oligosaccharides of rHPC were released by N-glycanase and separated into 25 fractions by high-pH anion-exchange chromatography. 27922006 1 1 gly glycoprotein 94:105 arg1 Myelin-associated glycoprotein Myelin-associated glycoprotein PUBTATOR Myelin-associated glycoprotein 4099 Myelin-associated glycoprotein (MAG) is a myelin-expressed cell-adhesion and bi-directional signalling molecule. 27922006 1 1 gly glycoprotein 94:105 arg1 MAG MAG PUBTATOR MAG 4099 Myelin-associated glycoprotein (MAG) is a myelin-expressed cell-adhesion and bi-directional signalling molecule. 8357534 4 44 gly contain 715:721 arg1 E-3 AND exclusively truncated, oligomannose-type chains E-3 and C-3 positions exclusively truncated, oligomannose-type chains PUBTATOR E-3 and C-3 P01024 positions In contrast, the isoforms E-3 and C-3, the major forms of elastase and cathepsin G respectively, contain exclusively truncated, oligomannose-type chains at the same positions in the sequence of each protein. 10386995 4 1 gly sites 727:731 arg1 synapsin I synapsin I sites PUBTATOR synapsin I 24949 The seven O-GlcNAcylation sites (Ser55, Thr56, Thr87, Ser516, Thr524, Thr562, and Ser576) in synapsin I are clustered around its five phosphorylation sites in domains B and D. 25374123 0 65 gly N-glycosylation 35:49 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 In-depth analysis of site-specific N-glycosylation in vitronectin from human plasma by tandem mass spectrometry with immunoprecipitation. 8193552 1 11 gly N-deglycosylated 297:312 arg1 the enzymically N-deglycosylated beta-subunit the enzymically N-deglycosylated beta-subunit OGER subunit P0DN86 The disialylated poly-(N-acetyllactosamine)-containing O-linked oligosaccharide alditols, released by alkaline borohydride treatment of the enzymically N-deglycosylated beta-subunit of equine chorionic gonadotropin, were purified by fast protein liquid chromatography (FPLC) on Mono Q and analysed by fast ion bombardment mass spectrometry (FAB-MS) and 1H-NMR spectroscopy. 21752865 5 65 gly N-glycosylated 1011:1024 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Given that N-glycosylation is considered essential for protein folding, processing and trafficking, we examined whether BRI2 is N-glycosylated. 19383836 4 27 gly glycosylation 570:582 arg1 ABCC11 ABCC11 PUBTATOR ABCC11 85320 We herein provide the evidence that this genetic polymorphism has an effect on the N-linked glycosylation of ABCC11, intracellular sorting, and proteasomal degradation of the variant protein. 11683872 3 23 gly glycosylated 528:539 arg1 VR1 VR1 PUBTATOR VR1 7442 VR1 was found to be glycosylated in both cell types. 10929010 3 13 gly transferrin 528:538 arg1 the N-glycan structures transferrin the N-glycan structures PUBTATOR transferrin 7018 Consequently, the N-glycan structures of transferrin in the culture medium were determined using three-dimensional high performance liquid chromatography. 8798614 3 69 part_of CD59 619:622 arg1 Asn18 CD59 Asn18 PUBTATOR AminoAcid CD59 966 Asn18 The deduced amino acid sequences of CD59 homologues identified in Old and New World primates as well as in rat reveal that the motif for N-linked glycosylation at the residue corresponding to Asn18 of human CD59 is invariably conserved, despite considerable sequence divergence elsewhere in the protein. 11258925 3 28 gly glycosylation 348:360 arg1 recombinant COX-2 recombinant COX-2 PUBTATOR COX-2 4513 This paper reports on the glycosylation site analysis of recombinant COX-2 using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) and nanoelectrospray (nanoESI) quadrupole-TOF (Q-TOF) MS. The nanoESI MS analysis of COX-2 revealed the presence of three glycoforms at average molecular masses of 71.4, 72.7, and 73.9 kDa. 16014566 0 77 gly glycoprotein 68:79 arg1 platelet glycoprotein VI platelet glycoprotein VI PUBTATOR platelet glycoprotein VI 51206 The influence of N-linked glycosylation on the function of platelet glycoprotein VI. 16014566 0 93 gly glycosylation 26:38 arg1 platelet glycoprotein VI platelet glycoprotein VI PUBTATOR platelet glycoprotein VI 51206 The influence of N-linked glycosylation on the function of platelet glycoprotein VI. 7505568 0 6 gly glycoprotein 70:81 arg1 human myelin-associated glycoprotein human myelin-associated glycoprotein PUBTATOR myelin-associated glycoprotein 4099 Identification of the glycosylated sequons of human myelin-associated glycoprotein. 9677334 11 70 part_of TPO 1513:1515 arg1 the Arg10 and Arg17 residues TPO the Arg10 and Arg17 residues PUBTATOR AminoAcid TPO 7066 Arg10 and Arg17 residues Moreover we found that the Arg10 and Arg17 residues of TPO seem to be specific determinants for TPO/c-Mpl recognition. 12911312 1 24 gly glycoprotein 227:238 arg1 Rat selenoprotein P Rat selenoprotein P PUBTATOR Rat selenoprotein P 29360 Rat selenoprotein P is an extracellular glycoprotein of 366 amino acid residues that is rich in cysteine and selenocysteine. 2971663 5 43 gly structures 1277:1286 arg1 leukosialin leukosialin structures PUBTATOR leukosialin P16150 Since both enzymes compete for the same precursor substrate, the coordinate changes in their activities are most likely responsible for the complete change of the carbohydrate structures on leukosialin during the activation of human T-lymphocytes. 23376777 7 59 gly deglycosylated 1413:1426 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 Under hypotonic stress conditions, the ability to adapt to changes in intracellular chloride ion concentrations and RVD (regulatory volume decrease) activities were less efficient in cells containing the deglycosylated form of KCC4 that were not expressed at the cell surface. 17925379 0 75 gly glycoproteins 30:42 arg1 Pannexin 1 Pannexin 1 PUBTATOR Pannexin 1 24145 Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins. 17925379 0 75 gly glycoproteins 30:42 arg1 pannexin 3 pannexin 3 PUBTATOR pannexin 3 116337 Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins. 17395589 0 38 gly O-fucosylation 0:13 arg1 ADAMTS13 secretion ADAMTS13 secretion PUBTATOR ADAMTS13 11093 O-fucosylation is required for ADAMTS13 secretion. 7107587 12 15 gly fibrinogen 1334:1343 arg1 greater than 95% biantennary oligosaccharide chains fibrinogen greater than 95% biantennary oligosaccharide chains PUBTATOR fibrinogen 2244 Affinity chromatography of the glycopeptides on concanavalin A-Sepharose also showed that the glycopeptides from fibrinogen are greater than 95% biantennary oligosaccharide chains. 7107587 12 67 gly glycopeptides 1315:1327 arg1 fibrinogen fibrinogen PUBTATOR fibrinogen 2244 Affinity chromatography of the glycopeptides on concanavalin A-Sepharose also showed that the glycopeptides from fibrinogen are greater than 95% biantennary oligosaccharide chains. 7776966 14 32 gly carbohydrates 2188:2200 arg1 the FSH receptor FSH receptor carbohydrates PUBTATOR FSH receptor 2492 Our results demonstrate that while N-linked carbohydrates on the FSH receptor are not required directly for the binding of hormone, a carbohydrate at either Asn174 or Asn276 is required for the efficient folding of the nascent receptor protein into a conformation that allows high affinity binding of hormone. 2498325 7 85 gly glycosylation 1122:1134 arg1 apoE apoE PUBTATOR apoE 348 Apolipoprotein E(Thr194----Ala) was secreted exclusively as the asialo isoform, confirming that Thr194 is the site of carbohydrate attachment in these cells and indicating that glycosylation of apoE is not essential for secretion. 7925474 2 16 gly glycosylation 247:259 arg1 fetal antigen 1 fetal antigen 1 PUBTATOR fetal antigen 1 8788 The present paper describes the primary structure, glycosylation and tissue localization of fetal antigen 1 (FA1) isolated from second-trimester human amniotic fluid. 7925474 2 16 gly glycosylation 247:259 arg1 FA1 FA1 PUBTATOR FA1 8788 The present paper describes the primary structure, glycosylation and tissue localization of fetal antigen 1 (FA1) isolated from second-trimester human amniotic fluid. 24365146 6 2 part_of Pro-156 1013:1019 arg1 p22 p22 Pro-156 PUBTATOR SpecificSite p22 11261 Pro-156 Superoxide production by unglycosylated Nox1 is largely dependent on p22(phox), which is abrogated by glutamine substitution for Pro-156 in p22(phox), a mutation leading to a defective interaction with the Nox1-activating protein Noxo1. 9054441 0 20 gly trisaccharide 41:53 arg1 Ser-248 Ser-248 Ser-248 SpecificSite Ser-248 Evidence for a novel O-linked sialylated trisaccharide on Ser-248 of human plasminogen 2. 19508227 0 14 gly Glycosylation 0:12 arg1 tetraspanin Tspan-1 tetraspanin Tspan-1 PUBTATOR Tspan-1 10103 Glycosylation of tetraspanin Tspan-1 at four distinct sites promotes its transition through the endoplasmic reticulum. 3497198 0 88 gly glycosylation 14:26 arg1 H-2K H-2K PUBTATOR H-2K 14972 Site specific glycosylation patterns of H-2K: effects of allelic polymorphism and mitogenic stimulation. 1323463 5 18 gly mucin 1099:1103 arg1 the acidic oligosaccharides mucin the acidic oligosaccharides PUBTATOR mucin 281333 The Lewis(y) and blood-group-A determinants of these sequences have not been found previously in the acidic oligosaccharides of bovine submaxillary-gland mucin, although they have recently been characterised in the neutral chains of bovine submaxillary-gland mucin. 7781780 1 7 gly glycosylation 234:246 arg1 recombinant human erythropoietin recombinant human erythropoietin PUBTATOR erythropoietin 2056 A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. 10878002 6 23 gly C-mannosylation 755:769 arg1 human properdin human properdin PUBTATOR properdin 5199 We studied the C-mannosylation pattern of human properdin by mass spectrometry and Edman degradation. 10878002 6 29 gly properdin 788:796 arg1 the C-mannosylation pattern properdin the C-mannosylation pattern PUBTATOR properdin 5199 We studied the C-mannosylation pattern of human properdin by mass spectrometry and Edman degradation. 7686446 3 6 gly AFP 697:699 arg1 Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2-->6Gal beta 1 -->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 AFP Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2-->6Gal beta 1 -->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 PUBTATOR AFP 174 Because a small amount of HCC-specific AFP isoform was detected in cord serum AFP, the whole sugar chain structures of human cord serum AFP were determined, as follows: Neu5Ac alpha 2-->Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2-->6Gal beta 1 -->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, and Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 in the ratio of 81.6:8.9:9.5. 7686446 3 6 gly AFP 697:699 arg1 Neu5Ac alpha 2-->Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 AFP Neu5Ac alpha 2-->Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 PUBTATOR AFP 174 Because a small amount of HCC-specific AFP isoform was detected in cord serum AFP, the whole sugar chain structures of human cord serum AFP were determined, as follows: Neu5Ac alpha 2-->Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2-->6Gal beta 1 -->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, and Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 in the ratio of 81.6:8.9:9.5. 7686446 3 6 gly AFP 697:699 arg1 the whole sugar chain structures AFP the whole sugar chain structures PUBTATOR AFP 174 Because a small amount of HCC-specific AFP isoform was detected in cord serum AFP, the whole sugar chain structures of human cord serum AFP were determined, as follows: Neu5Ac alpha 2-->Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2-->6Gal beta 1 -->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, and Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 in the ratio of 81.6:8.9:9.5. 7686446 3 6 gly AFP 697:699 arg1 Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc beta AFP Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc beta PUBTATOR AFP 174 Because a small amount of HCC-specific AFP isoform was detected in cord serum AFP, the whole sugar chain structures of human cord serum AFP were determined, as follows: Neu5Ac alpha 2-->Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2-->6Gal beta 1 -->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2, and Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->6(Neu5Ac alpha 2 -->6Gal beta 1-->4GlcNAc beta 1-->2Man alpha 1-->3)Man beta 1-->4R1 and R2 in the ratio of 81.6:8.9:9.5. 8349699 7 45 gly N-glycosylation 1047:1061 arg1 murine PGH synthase-2 murine PGH synthase-2 PUBTATOR PGH synthase-2 19225 Using site-directed mutagenesis, we determined that there is an additional site of N-glycosylation in murine PGH synthase-2 located at Asn580. 23236605 4 40 gly contains 724:731 arg1 human AIM AND no N-glycan human AIM no N-glycan OGER AIM Q07108 Although human AIM contains no N-glycan, attachment of N-glycans increased AIM secretion. 2403553 0 69 gly contains 27:34 arg1 Human transferrin receptor AND O-linked oligosaccharides Human transferrin receptor O-linked oligosaccharides PUBTATOR transferrin receptor 7018 Human transferrin receptor contains O-linked oligosaccharides. 24134926 1 74 gly present 193:199 arg2 ECM AND a glycosaminoglycan ECM a glycosaminoglycan OGER ECM Q13201 Hyaluronan (HA) is a glycosaminoglycan composed by repeating units of D-glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) that is ubiquitously present in the extracellular matrix (ECM) where it has a critical role in the physiology and pathology of several mammalian tissues. 10024660 4 44 gly present 711:717 arg1 BSSL AND esterified N- glycans BSSL esterified N- glycans PUBTATOR BSSL P19835 The molecular mass profile of esterified N- glycans present in BSSL further permitted the more detailed studies through collision-induced dissociation (CID) and sequential exoglycosidase cleavages. 16332679 1 77 gly O-glycosylation 171:185 arg1 Sp1 Sp1 OGER Sp1 P08047 O-glycosylation and phosphorylation of Sp1 are thought to modulate the expression of a number of genes in normal and diabetic state. 8142896 7 6 gly mass 1193:1196 arg1 a hexasaccharide mass 1,039 a hexasaccharide OGER mass 1,039 Q8WXG9 The predominant species was a hexasaccharide of molecular mass 1,039, containing a fucose subunit linked to the proximal N-acetylglucosamine residue: [formula: see text] 22750213 5 14 gly glycosylation 837:849 arg1 PRiMA PRiMA PUBTATOR PRiMA 170952 By using site-directed mutagenesis, the asparagine-43 was identified to be the N-linked glycosylation site of PRiMA. 27966990 7 25 gly deglycosylated 1146:1159 arg1 PNGase F-treated PNGase F-treated PUBTATOR PNGase 59007 MS/MS analyses of glycopeptides and deamidated, deglycosylated (PNGase F-treated) peptides from ectopically expressed VEGFR-2 in porcine aortic endothelial (PAE) cells identified N-glycans at the majority of the 17 potential N-glycosylation sites on VEGFR-2 in a site-specific manner. 10201933 1 19 gly glycoprotein 210:221 arg1 CDw108 CDw108 PUBTATOR CDw108 8482 CDw108, also known as the John-Milton-Hagen human blood group Ag, is an 80-kDa glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that is preferentially expressed on activated lymphocytes and E. 9933650 1 17 gly glycoprotein 81:92 arg1 Tamm-Horsfall glycoprotein Tamm-Horsfall glycoprotein PUBTATOR Tamm-Horsfall glycoprotein 7369 Tamm-Horsfall glycoprotein (THGP) and the oligosaccharide fraction liberated from THGP by hydrazinolysis inhibited tetanus toxoid-induced T cell proliferation. 9933650 1 17 gly glycoprotein 81:92 arg1 THGP THGP PUBTATOR THGP 7369 Tamm-Horsfall glycoprotein (THGP) and the oligosaccharide fraction liberated from THGP by hydrazinolysis inhibited tetanus toxoid-induced T cell proliferation. 9933650 1 43 gly liberated 134:142 arg2 THGP AND the oligosaccharide fraction THGP the oligosaccharide fraction PUBTATOR THGP 7369 Tamm-Horsfall glycoprotein (THGP) and the oligosaccharide fraction liberated from THGP by hydrazinolysis inhibited tetanus toxoid-induced T cell proliferation. 19285951 1 37 gly glycoprotein 128:139 arg1 HRG HRG PUBTATOR HRG 3273 Histidine-rich glycoprotein (HRG) is a plasma protein implicated in the innate immune system. 8665956 4 41 gly glycosylated 749:760 arg1 Sg II Sg II PUBTATOR Sg II 6407 To investigate the molecular interaction of Sg with PSA and PCI, we purified Sg II from seminal coagula as a soluble form and found that Sg II is glycosylated with heterogeneous carbohydrate moieties. 3667593 1 24 gly interferon-beta 186:200 arg1 the major oligosaccharide interferon-beta the major oligosaccharide PUBTATOR interferon-beta 3456 The carbohydrate structure of the major oligosaccharide of human interferon-beta (IFN-beta) synthesized by a genetically engineered Chinese hamster ovary cell line has been determined. 15863501 9 48 gly N-glycosylation 1604:1618 arg1 sPLA(2)-III sPLA(2)-III PUBTATOR sPLA(2)-III 50487 Taken together, these results reveal unique cell type-specific processing and N-glycosylation of sPLA(2)-III and the potential role of this enzyme in cancer development by stimulating tumor cell growth and angiogenesis. 14718370 10 72 gly C-mannosylated 1663:1676 arg1 MUC5B MUC5B PUBTATOR MUC5B Q9HC84 Considered together, these studies suggest that the Cys subdomains of MUC5AC and MUC5B are C-mannosylated in their respective WXXW motifs. 14718370 10 72 gly C-mannosylated 1663:1676 arg1 MUC5AC MUC5AC PUBTATOR MUC5AC P98088 Considered together, these studies suggest that the Cys subdomains of MUC5AC and MUC5B are C-mannosylated in their respective WXXW motifs. 7686446 0 57 gly alpha-fetoprotein 33:49 arg1 Sugar chains alpha-fetoprotein Sugar chains PUBTATOR alpha-fetoprotein 174 Sugar chains of human cord serum alpha-fetoprotein: characteristics of N-linked sugar chains of glycoproteins produced in human liver and hepatocellular carcinomas. 22766194 7 19 gly N-glycosylated 1402:1415 arg1 CD10 CD10 PUBTATOR CD10 4311 All of the three consensus sites of CD10 in HEK293 cells introduced with wild type-CD10 were confirmed to be N-glycosylated. 17715132 6 17 gly glycosylation 788:800 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 The glycosylation of Pannexin1 at its extracellular surface makes it unlikely that two oligomers could dock to form an intercellular channel. 25759508 0 41 gly O-Glycosylation 13:27 arg1 Human Immunoglobulin G3 Human Immunoglobulin G3 PUBTATOR Human Immunoglobulin G3 3502 Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3). 25759508 0 41 gly O-Glycosylation 13:27 arg1 IgG3 IgG3 PUBTATOR IgG3 P01860 Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3). 11904304 3 46 gly Thr-58-glycosylated 600:618 arg1 the Thr-58-glycosylated form form of c-Myc PUBTATOR form of c-Myc 4609 One antibody specifically reacts with the Thr-58-glycosylated form of c-Myc, and the other reacts only with unmodified Thr-58 in c-Myc. 8033893 7 69 gly O-glycosylation 1032:1046 arg1 rat platelet factor 4 rat platelet factor 4 OGER platelet factor 4 P06765 Overall consideration of these data led to identification of the higher-molecular-mass protein as a glycosylated form of rat platelet factor 4 with O-glycosylation at the second N-terminal amino acid, while the structure of the oligosaccharide core was established by mass spectrometry and sugar differentiation with lectins. 8033893 7 76 gly glycosylated 984:995 arg1 rat platelet factor 4 rat platelet factor 4 OGER platelet factor 4 P06765 Overall consideration of these data led to identification of the higher-molecular-mass protein as a glycosylated form of rat platelet factor 4 with O-glycosylation at the second N-terminal amino acid, while the structure of the oligosaccharide core was established by mass spectrometry and sugar differentiation with lectins. 21264968 3 84 gly glycoproteins 1252:1264 arg1 MUC5AC MUC5AC PUBTATOR MUC5AC 4586 An electron-capture dissociation device in a linear radio-frequency quadrupole ion trap (RFQ-ECD) combined with a time-of-flight (TOF) mass spectrometer was employed for the identification of Thr/Ser residues occupied by α-GalNAc branching among multiple and potential O-glycosylation sites in the tandem repeats of human mucin glycoproteins MUC4 (Thr-Ser-Ser-Ala-Ser-Thr-Gly-His-Ala-Thr-Pro-Leu-Pro-Val-Thr-Asp) and MUC5AC (Pro-Thr-Thr-Val-Gly-Ser-Thr-Thr-Val-Gly). 21264968 3 84 gly glycoproteins 1252:1264 arg1 MUC4 MUC4 PUBTATOR MUC4 4585 An electron-capture dissociation device in a linear radio-frequency quadrupole ion trap (RFQ-ECD) combined with a time-of-flight (TOF) mass spectrometer was employed for the identification of Thr/Ser residues occupied by α-GalNAc branching among multiple and potential O-glycosylation sites in the tandem repeats of human mucin glycoproteins MUC4 (Thr-Ser-Ser-Ala-Ser-Thr-Gly-His-Ala-Thr-Pro-Leu-Pro-Val-Thr-Asp) and MUC5AC (Pro-Thr-Thr-Val-Gly-Ser-Thr-Thr-Val-Gly). 21264968 3 128 gly glycoproteins 1252:1264 arg1 the tandem repeats mucin glycoproteins the tandem repeats PUBTATOR mucin glycoproteins 100508689 An electron-capture dissociation device in a linear radio-frequency quadrupole ion trap (RFQ-ECD) combined with a time-of-flight (TOF) mass spectrometer was employed for the identification of Thr/Ser residues occupied by α-GalNAc branching among multiple and potential O-glycosylation sites in the tandem repeats of human mucin glycoproteins MUC4 (Thr-Ser-Ser-Ala-Ser-Thr-Gly-His-Ala-Thr-Pro-Leu-Pro-Val-Thr-Asp) and MUC5AC (Pro-Thr-Thr-Val-Gly-Ser-Thr-Thr-Val-Gly). 7776966 12 45 gly nonglycosylated 1952:1966 arg1 the resulting nonglycosylated FSHR the resulting nonglycosylated FSHR PUBTATOR FSHR 2492 Similarly, when cells expressing the wild type FSHR were treated with tunicamycin to prevent N-linked glycosylation, the resulting nonglycosylated FSHR was not able to bind FSH. 26013384 8 53 part_of Ser126 1023:1028 arg1 fully O-glycosylated rHu-EPO EPO Ser126 PUBTATOR AminoAcid EPO 2056 Ser126 It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126. 2386787 1 11 gly plasminogen 221:231 arg1 the Asn289-linked oligosaccharide structures plasminogen the Asn289-linked oligosaccharide structures OGER plasminogen P00747 A comparison has been made between the Asn289-linked oligosaccharide structures of human plasma plasminogen and a recombinant human plasminogen, expressed in lepidopteran insect (Spodoptera frugiperda) cells, after infection of these cells with a recombinant baculovirus containing the entire human plasminogen cDNA. 2386787 1 45 gly plasminogen 257:267 arg1 the Asn289-linked oligosaccharide structures plasminogen the Asn289-linked oligosaccharide structures OGER plasminogen P00747 A comparison has been made between the Asn289-linked oligosaccharide structures of human plasma plasminogen and a recombinant human plasminogen, expressed in lepidopteran insect (Spodoptera frugiperda) cells, after infection of these cells with a recombinant baculovirus containing the entire human plasminogen cDNA. 12970363 10 21 gly monoglycosylated 1432:1447 arg1 the monoglycosylated TRPC3 the monoglycosylated TRPC3 PUBTATOR TRPC3 7222 Elimination of the e2 glycosylation site, missing in the monoglycosylated TRPC3, was sufficient to convert the tightly receptor-regulated TRPC6 into a constitutively active channel, displaying functional characteristics of TRPC3. 11983428 1 35 gly glycoprotein 241:252 arg1 Stromal interaction molecule 1 Stromal interaction molecule 1 PUBTATOR Stromal interaction molecule 1 6786 Stromal interaction molecule 1 (STIM1) is a cell surface transmembrane glycoprotein implicated in tumour growth control and stromal-haematopoietic cell interactions. 9757569 2 26 gly glycosylated 391:402 arg1 S14N angiotensinogen S14N angiotensinogen PUBTATOR S14N angiotensinogen P01019 The molecular weight was about 3,000 larger than that of wild-type ovine angiotensinogen, indicating that S14N angiotensinogen was glycosylated at Asn14. 18642129 7 20 gly N-glycosylated 1081:1094 arg1 heavy chain chain OGER chain P08709 LC-ESIMS/MS analysis revealed that both light chain and heavy chain were N-glycosylated mainly by A2S2 but also by triantennary sialylated glycans. 18795891 10 44 gly glycoprotein 1602:1613 arg1 the collagen receptor GPVI (glycoprotein VI) and integrin alpha6beta1, but not the von Willebrand receptor GPIbalpha the collagen receptor GPVI (glycoprotein VI) and integrin alpha6beta1, but not the von Willebrand receptor GPIbalpha PUBTATOR glycoprotein VI 51206 Finally, Tspan9 was shown to be a component of tetraspanin microdomains that included the collagen receptor GPVI (glycoprotein VI) and integrin alpha6beta1, but not the von Willebrand receptor GPIbalpha or the integrins alphaIIbbeta3 or alpha2beta1. 21264968 4 27 gly glycosylated 1611:1622 arg1 densely glycosylated MUC4 densely glycosylated MUC4 PUBTATOR MUC4 4585 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 gly containing 1629:1638 arg1 densely glycosylated MUC4 AND six α-GalNAc residues densely glycosylated MUC4 Thr15 six α-GalNAc residues PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 16321355 0 33 gly N-glycosylation 14:28 arg1 human plasma ceruloplasmin human plasma ceruloplasmin PUBTATOR ceruloplasmin 1356 Site-specific N-glycosylation analysis of human plasma ceruloplasmin using liquid chromatography with electrospray ionization tandem mass spectrometry. 21676880 4 34 gly unglycosylated 870:883 arg1 unglycosylated KCNE1 subunits unglycosylated KCNE1 subunits PUBTATOR KCNE1 subunits 3753 Mutations that ablate the co-translational site concomitantly reduce glycosylation at the post-translational site, resulting in unglycosylated KCNE1 subunits that cannot reach the cell surface with their cognate K(+) channel. 1991473 2 0 gly N-glycosylated 268:281 arg1 hLH beta hLH beta PUBTATOR hLH beta 3972 hLH alpha (N-glycosylated at Asn52 and Asn78) and hLH beta (N-glycosylated at Asn30). 1991473 2 11 gly N-glycosylated 219:232 arg1 hLH alpha hLH alpha PUBTATOR hLH alpha 1081 hLH alpha (N-glycosylated at Asn52 and Asn78) and hLH beta (N-glycosylated at Asn30). 15809306 7 59 part_of Ala 1037:1039 arg1 FAPalpha FAPalpha Ala PUBTATOR SpecificSite FAPalpha 2191 Ala(657) Ala(657) in FAPalpha, instead of Asp(663) as in DP-PIV, reduces the acidity in this pocket, and this change could explain the lower affinity for N-terminal amines by FAPalpha. 15677325 1 39 gly glycosylated 138:149 arg1 the kainate receptor subunit GluR6 the kainate receptor subunit GluR6 OGER subunit GluR6 2898 We report the crystal structure of the glycosylated ligand-binding (S1S2) domain of the kainate receptor subunit GluR6, in complex with the agonist domoate. 10712595 0 8 gly has 20:22 arg1 Salmon antithrombin AND only three carbohydrate side chains Salmon antithrombin only three carbohydrate side chains PUBTATOR antithrombin 462 Salmon antithrombin has only three carbohydrate side chains, and shows functional similarities to human beta-antithrombin. 8407981 0 51 gly glycosylation 5:17 arg1 cytochrome P-450 cytochrome P-450(arom) PUBTATOR cytochrome P-450(arom) 55010 Core glycosylation of cytochrome P-450(arom). 7525874 0 27 gly protein 38:44 arg1 Carbohydrate structures beta-trace protein Carbohydrate structures PUBTATOR beta-trace protein 5730 Carbohydrate structures of beta-trace protein from human cerebrospinal fluid: evidence for "brain-type" N-glycosylation. 3651384 1 44 gly glycoprotein 102:113 arg1 SGP-2 SGP-2 PUBTATOR 2 (SGP-2 24854 Sulfated glycoprotein 2 (SGP-2) is the major protein secreted by rat Sertoli cells. 3651384 1 44 gly glycoprotein 102:113 arg1 Sulfated glycoprotein 2 Sulfated glycoprotein 2 PUBTATOR Sulfated glycoprotein 2 24854 Sulfated glycoprotein 2 (SGP-2) is the major protein secreted by rat Sertoli cells. 1991473 3 27 gly liberated 316:324 arg1 intact hLH beta AND The sugar chains intact hLH beta The sugar chains PUBTATOR hLH beta 3972 The sugar chains were liberated by hydrazinolysis from intact hLH beta and from glycopeptides obtained after tryptic digestion of hLH alpha, subsequently reduced and fractionated as alditols by anion-exchange and ion-suppression amine-adsorption HPLC and identified mainly by one-dimensional (1D) and two-dimensional (2D) 1H-NMR spectroscopy. 28327546 7 60 gly glycosylation 890:902 arg1 wild-type FVIII wild-type FVIII PUBTATOR FVIII 2157 Selected mutations also lead to partial glycosylation of N582, suggesting that rapid folding of local conformation prevents glycosylation of this site in wild-type FVIII. 10386995 5 8 gly sites 935:939 arg1 synapsin I synapsin I sites PUBTATOR synapsin I 24949 The proximity of phosphorylation sites to O-GlcNAcylation sites in the regulatory domains of synapsin I suggests that O-GlcNAcylation may modulate phosphorylation and indirectly affect synapsin I interactions. 7096333 0 0 gly ceruloplasmin 81:93 arg1 the carbohydrate chains ceruloplasmin the carbohydrate chains PUBTATOR ceruloplasmin 1356 The structures and microheterogeneity of the carbohydrate chains of human plasma ceruloplasmin. 22826440 1 27 gly found 184:188 arg1 the neuronal protein AP180 AND Phosphorylated O-GlcNAc the neuronal protein AP180 Phosphorylated O-GlcNAc PUBTATOR AP180 65178 Phosphorylated O-GlcNAc is a novel post-translational modification that has so far only been found on the neuronal protein AP180 from the rat (Graham et al., J. Proteome Res. 3356193 5 38 gly NeuAc 718:722 arg1 porcine plasminogen plasminogen NeuAc OGER plasminogen P00747 In porcine plasminogen the sialic acid is mainly NeuAc; the Man alpha 1----6 branch, however, is only partially sialylated. 17534424 0 116 gly glycoprotein 32:43 arg1 Human ClC-6 Human ClC-6 PUBTATOR ClC-6 1185 Human ClC-6 is a late endosomal glycoprotein that associates with detergent-resistant lipid domains. 7727388 0 91 gly glycosylation 27:39 arg1 the human natriuretic peptide receptor-C homodimer the human natriuretic peptide receptor-C homodimer PUBTATOR natriuretic peptide receptor-C homodimer 4883 The disulfide linkages and glycosylation sites of the human natriuretic peptide receptor-C homodimer. 19343721 1 75 gly macro-heterogeneity 256:274 arg1 plasma-derived hPC plasma-derived hPC OGER hPC P11498 We have characterized the micro- and macro-heterogeneity of plasma-derived hPC and compared the glycosylation features with recombinant protein C (tg-PC) produced in a transgenic pig bioreactor from two animals having approximately tenfold different expression levels. 24125761 3 34 gly glycosylation 341:353 arg1 AADAC enzyme activity AADAC enzyme PUBTATOR AADAC enzyme 13 In the present study, we investigated the effect of glycosylation on AADAC enzyme activity. 18952059 3 36 gly unglycosylated 302:315 arg1 unglycosylated IZUMO unglycosylated IZUMO PUBTATOR IZUMO 73456 In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. 18952059 3 36 gly unglycosylated 302:315 arg1 N204Q-IZUMO N204Q-IZUMO PUBTATOR -IZUMO 73456 In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. 11706042 2 29 gly TLR4 355:358 arg1 N-linked carbohydrates TLR4 N-linked carbohydrates PUBTATOR TLR4 7099 Because each of these proteins is glycosylated, we have examined the functional role of N-linked carbohydrates of both MD-2 and TLR4. 11706042 2 32 gly MD-2 346:349 arg1 N-linked carbohydrates MD-2 N-linked carbohydrates OGER MD-2 Q9Y6Y9 Because each of these proteins is glycosylated, we have examined the functional role of N-linked carbohydrates of both MD-2 and TLR4. 1323463 1 38 gly released 186:193 arg1 bovine submaxillary-gland mucin AND The acidic oligosaccharide alditols bovine submaxillary-gland mucin The acidic oligosaccharide alditols PUBTATOR mucin 281333 The acidic oligosaccharide alditols released from bovine submaxillary-gland mucin by Carlson degradation were investigated by a combination of liquid secondary-ion mass spectrometry, methylation analysis and 1H-NMR. 19952283 1 30 gly glycoprotein 107:118 arg1 Neuroplastin Neuroplastin OGER Neuroplastin P97546 Neuroplastin (Np) is a glycoprotein belonging to the immunoglobulin superfamily of cell adhesion molecules (CAMs) and existing in two isoforms, Np55 and Np65, named according to their molecular weights. 15054092 5 40 gly glycosylation 715:727 arg1 G8 G8 Cterm G8 Q9H221 N-Linked glycosylation of G8 was required for efficient trafficking of the G5/G8 heterodimer, but mutations that abolished glycosylation of G5 did not prevent trafficking of the heterodimer. 15054092 5 43 gly glycosylation 829:841 arg1 G5 G5 Cterm G5 Q9H222 N-Linked glycosylation of G8 was required for efficient trafficking of the G5/G8 heterodimer, but mutations that abolished glycosylation of G5 did not prevent trafficking of the heterodimer. 20739279 0 45 gly N-glycosylation 0:14 arg1 synaptic cell adhesion molecule synaptic cell adhesion molecule PUBTATOR synaptic cell adhesion molecule 23705 N-glycosylation at the SynCAM (synaptic cell adhesion molecule) immunoglobulin interface modulates synaptic adhesion. 27384988 8 17 gly Concanavalin 809:820 arg1 a carbohydrate binding lectin protein Concanavalin A a carbohydrate binding lectin protein Cterm Concanavalin A RpS3 bound to Concanavalin A, a carbohydrate binding lectin protein, while treatment with peptide-N-glycosidase F shifted the secreted rpS3 to a lower molecular weight band. 17534424 3 19 gly N-glycosylated 766:779 arg1 human ClC-6 human ClC-6 PUBTATOR ClC-6 1185 PRINCIPAL FINDINGS: Using a polyclonal affinity-purified antibody directed against a unique epitope in the ClC-6 COOH-terminal tail, we show that human ClC-6, when transfected in COS-1 cells, is N-glycosylated in a region that is evolutionary poorly conserved between mammalian CLC proteins and that is located between the predicted helices K and M. Three asparagine residues (N410, N422 and N432) have been defined by mutagenesis as acceptor sites for N-glycosylation, but only two of the three sites seem to be simultaneously N-glycosylated. 10878002 0 13 gly C-mannosylated 59:72 arg1 Properdin Properdin PUBTATOR Properdin 5199 Properdin, the positive regulator of complement, is highly C-mannosylated. 27294781 0 39 gly glycosylation 9:21 arg1 SV2 SV2 PUBTATOR SV2 9900 N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A. Botulinum neurotoxin serotype A1 (BoNT/A1), a licensed drug widely used for medical and cosmetic applications, exerts its action by invading motoneurons. 1883960 4 18 gly O-glycosylated 1037:1050 arg1 25-kD O-glycosylated IL-6 25-kD O-glycosylated IL-6 PUBTATOR IL-6 3569 Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30, suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site. 1883960 4 89 gly O-glycosylated 1130:1143 arg1 the 28- to 30-kD N- and O-glycosylated IL-6 the 28- to 30-kD N- and O-glycosylated IL-6 PUBTATOR IL-6 3569 Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30, suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site. 22556278 0 26 gly Hsp90 58:62 arg1 O-GlcNAc sites Hsp90 O-GlcNAc sites PUBTATOR Hsp90 P08238 Mapping of O-GlcNAc sites of 20 S proteasome subunits and Hsp90 by a novel biotin-cystamine tag. 24342833 0 44 gly glycosylated 22:33 arg1 β-catenin β-catenin PUBTATOR -catenin 1499 β-catenin is O-GlcNAc glycosylated at Serine 23: implications for β-catenin's subcellular localization and transactivator function. 9530955 12 22 gly glycoproteins 2078:2090 arg1 MUC1 glycoproteins MUC1 glycoproteins PUBTATOR MUC1 glycoproteins 4582 The remarkable similarities in the total carbohydrate content, the carbohydrate composition and structures of saccharides between epitectin from urine, a non-malignant source, and H.Ep.2 cells is surprising in view of the prevailing view that MUC1 glycoproteins of cancer cells are underglycosylated compared to those produced by non-malignant cells. 9530955 12 69 gly underglycosylated 2112:2128 arg1 MUC1 glycoproteins MUC1 glycoproteins PUBTATOR MUC1 glycoproteins 4582 The remarkable similarities in the total carbohydrate content, the carbohydrate composition and structures of saccharides between epitectin from urine, a non-malignant source, and H.Ep.2 cells is surprising in view of the prevailing view that MUC1 glycoproteins of cancer cells are underglycosylated compared to those produced by non-malignant cells. 19017259 4 18 gly structures 784:793 arg1 rTFPI rTFPI structures PUBTATOR rTFPI 29436 Sugar chain structures in rTFPI expressed in Chinese hamster ovary (CHO) cells have been reported previously, but those of plasma TFPI have not been. 17018531 2 28 gly glycoprotein 340:351 arg1 NPC2 NPC2 OGER NPC2 Q9Z0J0 One form of the disease is caused by a deficiency in NPC2, a soluble lysosomal glycoprotein that binds cholesterol. 19690161 4 58 part_of vIL-6 538:542 arg1 The Asn-89 site vIL The Asn-89 site OGER SpecificSite vIL P09327 Asn-89 site The Asn-89 site of vIL-6, found to be required for optimal cytokine function, is composed of complex glycans. 8130392 13 38 gly rHPC 1921:1924 arg1 the Asn-linked oligosaccharides rHPC the Asn-linked oligosaccharides OGER rHPC P52873 Biochem., 16D, 151] observed in the Asn-linked oligosaccharides of rHPC derived from human kidney 293 cells, we propose to label the GalNAc beta-(1-->4)[Fuc alpha (1-->3)]GlcNAc beta (1-->.) 26412745 3 28 part_of AKT 516:518 arg1 Ser473 AKT Ser473 PUBTATOR AminoAcid AKT 11651 Thr308 and Ser473 By using co-immunoprecipitation and mutagenesis techniques, we identified O-GlcNAc modification at both Thr308 and Ser473 of AKT. 30582698 5 32 gly MGL 680:682 arg1 this glycan specificity MGL this glycan specificity PUBTATOR MGL 10462 Even though this glycan specificity of MGL is well described, there is a lack of understanding of the actual glycoproteins that bind MGL. 21264968 7 89 gly glycosylation 2369:2381 arg1 MUC5AC MUC5AC PUBTATOR MUC5AC 4586 Such conformational impact on the underlying peptides was proved to be remarkable in the glycosylation at the consecutive Thr residues of MUC5AC. 9524075 9 36 gly non-glycosylated 1048:1063 arg1 the glycosylated and non-glycosylated procathepsin S the glycosylated and non-glycosylated procathepsin S Cterm the glycosylated and non-glycosylated procathepsin S 1520 A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. 9524075 9 55 gly glycosylated 1031:1042 arg1 the glycosylated and non-glycosylated procathepsin S the glycosylated and non-glycosylated procathepsin S Cterm the glycosylated and non-glycosylated procathepsin S 1520 A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. 12218058 1 1 gly glycosylated 160:171 arg1 Bone morphogenetic protein (BMP)-1 Bone morphogenetic protein (BMP)-1 PUBTATOR Bone morphogenetic protein (BMP)-1 649 Bone morphogenetic protein (BMP)-1 is a glycosylated metalloproteinase that is fundamental to the synthesis of a normal extracellular matrix because it cleaves type I procollagen, as well as other precursor proteins. 1567356 1 10 gly contains 95:102 arg1 Orosomucoid AND tetra-antennary glycan chains Orosomucoid (OMD tetra-antennary glycan chains OGER Orosomucoid (OMD Q99983 Orosomucoid (OMD) contains complex bi-, tri- and tetra-antennary glycan chains. 1567356 1 10 gly contains 95:102 arg1 Orosomucoid AND tri- Orosomucoid (OMD tri- OGER Orosomucoid (OMD Q99983 Orosomucoid (OMD) contains complex bi-, tri- and tetra-antennary glycan chains. 1567356 1 10 gly contains 95:102 arg1 Orosomucoid AND complex bi- Orosomucoid (OMD complex bi- OGER Orosomucoid (OMD Q99983 Orosomucoid (OMD) contains complex bi-, tri- and tetra-antennary glycan chains. 29226084 6 0 gly N-glycosylation 1199:1213 arg1 GPR61 GPR61 OGER GPR61 Q9BZJ8 These results demonstrate that GPR61 is subject to N-glycosylation but suggest this is not a prerequisite for cell surface expression, although N-glycosylation of other proteins may be important for cell membrane expression of GPR61. 24161696 5 88 gly N-glycosylation 967:981 arg1 Kv3.1b Kv3.1b Cterm Kv3.1b RESULTS: Microscopy images revealed that occupancy of both N-glycosylation sites of Kv3.1b had relatively similar amounts of Kv3.1b in the outgrowth and cell body while vacancy of one or both sites led to increased accumulation of Kv3.1b in the cell body. 2108149 1 74 gly possessing 327:336 arg1 different IgGs AND structurally distinct CH2-linked oligosaccharide moieties different IgGs structurally distinct CH2-linked oligosaccharide moieties Cterm IgGs To examine the nature of the factors influencing the galactosylation pattern of the heavy chain of murine immunoglobulin G (IgG), cell fusion was performed between a myeloma (P3x63Ag8) and a hybridoma (Sp2HL/Bu) cell line which secrete different IgGs possessing structurally distinct CH2-linked oligosaccharide moieties. 25617829 3 18 gly carries 520:526 arg1 the nutrient-sensing class C G protein-coupled receptor GPRC6A AND seven N-glycans the nutrient-sensing class C G protein-coupled receptor GPRC6A seven N-glycans PUBTATOR GPRC6A 222545 Herein, we show that the nutrient-sensing class C G protein-coupled receptor GPRC6A carries seven N-glycans and that one of these sites modulates surface expression whereas mutation of another site affects receptor function. 29932112 10 20 gly un-glycosylated 1353:1367 arg1 Panx1 Panx1 PUBTATOR Panx1 and 2 24145 We found that the un-glycosylated forms of Panx1 and 2 can readily interact, regulating their localization and potentially their channel function in cells where they are co-expressed. 9524075 11 66 gly non-glycosylated 1245:1260 arg1 non-glycosylated procathepsin S non-glycosylated procathepsin S Cterm non-glycosylated procathepsin S 1520 Subcellular fractionation showed non-glycosylated procathepsin S in the membrane fraction. 19119025 1 43 gly glycoprotein 129:140 arg1 Tapasin Tapasin PUBTATOR Tapasin 6892 Tapasin is a glycoprotein critical for loading major histocompatibility complex (MHC) class I molecules with high-affinity peptides. 16452088 8 63 gly modified 1384:1391 arg1 Piccolo AND O-GlcNAc Piccolo O-GlcNAc Cterm Piccolo Bassoon and Piccolo, proteins critical to synapse assembly and vesicle docking, were extensively modified by O-GlcNAc. 23001782 3 36 gly glycosylation 495:507 arg1 serum-derived hSHBG serum-derived hSHBG PUBTATOR hSHBG 6462 Here, we perform a detailed site-specific characterization of the N- and O-linked glycosylation of serum-derived hSHBG. 1991473 6 15 gly attached 776:783 arg1 hLH alpha AND The oligosaccharides hLH alpha The oligosaccharides PUBTATOR hLH alpha 1081 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 1991473 6 15 gly attached 776:783 arg1 hLH beta AND The oligosaccharides hLH beta The oligosaccharides PUBTATOR hLH beta 3972 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 15454184 4 11 part_of contains 509:516 arg1 FS AND two potential N-glycosylation sites FS sites, Asn95 and Asn259 Cterm AminoAcid FS 10468 sites, Asn95 and Asn259 FS contains two potential N-glycosylation sites, Asn95 and Asn259. 15454184 4 11 part_of contains 509:516 arg1 FS AND Asn259 FS sites, Asn95 and Asn259 Cterm AminoAcid FS 10468 sites, Asn95 and Asn259 FS contains two potential N-glycosylation sites, Asn95 and Asn259. 15454184 4 11 part_of contains 509:516 arg1 FS AND Asn259 FS sites, Asn95 and Asn259 Cterm AminoAcid FS 10468 sites, Asn95 and Asn259 FS contains two potential N-glycosylation sites, Asn95 and Asn259. 2136357 3 26 gly glycoprotein 1033:1044 arg1 Tamm-Horsfall glycoprotein Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 In this work, we have extended the observation of pregnancy-associated inhibitory activity to a second species, and have compared the oligomannose profile of Tamm-Horsfall glycoprotein (nonpregnant) with that of uromodulin (pregnant) derived from both human and bovine sources. 1457969 2 72 gly glycosylated 414:425 arg1 hTSH beta hTSH beta PUBTATOR hTSH beta 7252 Highly purified, biologically active human thyrotrophin (hTSH) was dissociated into its subunits hTSH alpha (glycosylated at Asn 52 and Asn 78) and hTSH beta (glycosylated at Asn 23). 1457969 2 85 gly glycosylated 364:375 arg1 hTSH alpha hTSH alpha PUBTATOR hTSH alpha 1081 Highly purified, biologically active human thyrotrophin (hTSH) was dissociated into its subunits hTSH alpha (glycosylated at Asn 52 and Asn 78) and hTSH beta (glycosylated at Asn 23). 9030779 2 63 gly sphingomyelinase 379:394 arg1 the six potential N-linked oligosaccharide chains acid sphingomyelinase the six potential N-linked oligosaccharide chains PUBTATOR acid sphingomyelinase 6609 We have determined the influence of the six potential N-linked oligosaccharide chains of human acid sphingomyelinase (ASM) on catalytic activity, targeting, and processing of the enzyme. 11152678 2 20 gly N-glycosylated 434:447 arg1 pro-BDNF pro-BDNF PUBTATOR AminoAcid BDNF 627 Metabolic labeling, immunoprecipitation, and SDS-polyacrylamide gel electrophoresis reveal that pro-BDNF is generated as a 32-kDa precursor that is N-glycosylated and glycosulfated on a site, within the pro-domain. 12545205 0 97 gly N-cadherin 25:34 arg1 Carbohydrate moieties N-cadherin Carbohydrate moieties PUBTATOR N-cadherin 1000 Carbohydrate moieties of N-cadherin from human melanoma cell lines. 19141282 3 9 gly unglycosylated 454:467 arg1 unglycosylated IL-7Ralpha unglycosylated IL-7Ralpha PUBTATOR IL-7Ralpha 3575 IL-7 binds glycosylated IL-7Ralpha 300-fold more tightly than unglycosylated IL-7Ralpha, and the enhanced affinity is attributed primarily to an accelerated on rate. 19141282 3 31 gly glycosylated 403:414 arg1 glycosylated IL-7Ralpha glycosylated IL-7Ralpha PUBTATOR IL-7Ralpha 3575 IL-7 binds glycosylated IL-7Ralpha 300-fold more tightly than unglycosylated IL-7Ralpha, and the enhanced affinity is attributed primarily to an accelerated on rate. 26811476 7 0 gly glycoprotein 1322:1333 arg1 glycoprotein 2 glycoprotein 2 PUBTATOR glycoprotein 2 2813 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 26811476 7 0 gly glycoprotein 1322:1333 arg1 GP2 GP2 PUBTATOR GP2 2813 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 26811476 7 52 gly glycoproteins 1285:1297 arg1 glycoprotein 2 glycoprotein 2 PUBTATOR glycoprotein 2 2813 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 26811476 7 52 gly glycoproteins 1285:1297 arg1 α-tectorin α-tectorin PUBTATOR -tectorin 7007 This arrangement is required for filament formation and is directed by an ordered ZP-N/ZP-C linker that is not observed in ZP2 but is conserved in the sequence of deafness/Crohn's disease-associated homopolymeric glycoproteins α-tectorin (TECTA) and glycoprotein 2 (GP2). 1374031 2 13 gly subunit 246:252 arg1 The N-linked carbohydrate chains subunit The N-linked carbohydrate chains OGER subunit P0DN86 The N-linked carbohydrate chains of the beta subunit of human chorionic gonadotropin (hCG-beta) isolated from the culture fluid of the choriocarcinoma cell line BeWo were released enzymatically by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. Subsequently, the O-linked oligosaccharides were split off from the N-deglycosylated protein by mild alkaline borohydride treatment. 28668641 1 34 gly glycoprotein 128:139 arg1 Vaspin Vaspin PUBTATOR Vaspin 145264 Vaspin is a glycoprotein with three predicted glycosylation sites at asparagine residues located in proximity to the reactive center loop and close to domains that play important roles in conformational changes underlying serpin function. 11683872 2 41 gly glycosylation 321:333 arg1 VR1 VR1 PUBTATOR structure of VR1 7442 Here we describe its biochemical properties and assess the subcellular localization, the glycosylation state and the quaternary structure of VR1 expressed in HEK293 cells and in the DRG-derived cell line F-11 (N18TG2 mouse neuroblastoma x rat dorsal root ganglia, hybridoma). 10207176 6 9 gly C-mannosylated 895:908 arg1 nonrecombinant IL-12 nonrecombinant IL-12 OGER IL-12 This shows that nonrecombinant IL-12 is potentially C-mannosylated as well. 14749323 4 67 gly glycosylation 629:641 arg1 mOAT1 function mOAT1 function PUBTATOR mOAT1 18399 274, 1519-1524) that tunicamycin, an inhibitor of asparagine-linked glycosylation, significantly inhibited organic anion transport in COS-7 cells expressing a mouse organic anion transporter (mOAT1), suggesting an important role of glycosylation in mOAT1 function. 7541354 1 40 gly present 117:123 arg1 human plasma vitronectin AND N-linked oligosaccharides human plasma vitronectin N-linked oligosaccharides PUBTATOR vitronectin 7448 The structures of N-linked oligosaccharides present on human plasma vitronectin were elucidated. 10029548 7 67 gly glycosylation 1087:1099 arg1 hTF/2N hTF/2N OGER hTF P02787 Because of its distance from the iron-binding site, glycosylation of Ser-32 should not affect the iron-binding properties of hTF/2N expressed in P. pastoris, making this an excellent expression system for the production of hTF/2N. 27384988 0 42 gly glycosylated 75:86 arg1 Ribosomal protein S3 Ribosomal protein S3 PUBTATOR Ribosomal protein S3 6188 Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated. 27384988 0 42 gly glycosylated 75:86 arg1 rpS3 rpS3 PUBTATOR rpS3 6188 Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated. 21757702 2 16 gly sites 297:301 arg1 mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2) mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2) mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2) SpecificSite sequence C(1) Here we examine the occupancy of the predicted O-glucose sites on mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2). 1517205 0 64 gly has 16:18 arg1 Human factor IX AND a tetrasaccharide Human factor IX a tetrasaccharide OGER factor IX P00740 Human factor IX has a tetrasaccharide O-glycosidically linked to serine 61 through the fucose residue. 19088065 12 35 gly sialylation 1587:1597 arg1 KLK6 KLK6 Cterm KLK6 Therefore, the extensive and almost exclusive sialylation of KLK6 from ovarian cancer cells could lead to the development of an improved biomarker for the early diagnosis of ovarian carcinoma. 19088065 12 85 gly KLK6 1602:1605 arg1 the extensive and almost exclusive sialylation KLK6 the extensive and almost exclusive sialylation Cterm KLK6 Therefore, the extensive and almost exclusive sialylation of KLK6 from ovarian cancer cells could lead to the development of an improved biomarker for the early diagnosis of ovarian carcinoma. 17563389 5 60 gly nonglycosylated 1021:1035 arg1 nonglycosylated NK1R nonglycosylated NK1R PUBTATOR NK1R 6869 All mutant receptors were able to bind to substance P and neurokinin A ligand with similar affinities; however, the double mutant, nonglycosylated NK1R showed only half the B(max) of the wild-type NK1R. 2387072 5 44 gly glycoprotein 1218:1229 arg1 fibronectin fibronectin PUBTATOR fibronectin 2335 Glycopeptides derived from a glycoprotein, fibronectin, secreted from CF fibroblasts were also examined by 1H-NMR spectroscopy and showed no evidence of fucosyl residues linked alpha 1----3 to branch N-acetylglucosamine and a lesser percentage of core fucose than found in the peripheral membrane glycopeptides. 14764083 9 9 part_of Asn281 1308:1313 arg1 bLF-A bLF Asn281 Cterm AminoAcid bLF 3131 Asn281 This difference is due to glycosylation at Asn281 in bLF-A. 1457969 8 18 gly hTSH 1609:1612 arg1 the N-glycans hTSH the N-glycans OGER hTSH Some interesting structural features, not previously reported for the N-glycans of hTSH, included 3-O-sulphated galactose (SO4-3Gal) and peripheral fucose (Fuc alpha 1-3GlcNAc) in the Man alpha 1-6 branch of some diantennary structures; the former suggests the presence of a hitherto uncharacterized galactose-3-O-sulphotransferase in thyrotroph cells of the human anterior pituitary gland. 18467335 4 15 gly heterogeneity 486:498 arg1 blood-derived PCI blood-derived PCI OGER PCI P05154 In this study we have for the first time provided a full explanation for the marked size heterogeneity of blood-derived PCI and identified functional differences between naturally occurring PCI variants. 12604466 7 6 gly glycosylated 1089:1100 arg1 NBCe1 NBCe1 OGER NBCe1 Q9Y6R1 Immunoblotting of oocyte membrane extracts treated with PNGase F indicates that NBCe1 is normally glycosylated at N597 and N617 (both on the third extracellular loop). 3264725 1 7 gly glycoprotein 186:197 arg1 Blood coagulation factor VII Blood coagulation factor VII OGER coagulation factor VII P08709 Blood coagulation factor VII is a vitamin K dependent glycoprotein which in its activated form, factor VIIa, participates in the coagulation process by activating factor X and/or factor IX in the presence of Ca2+ and tissue factor. 1694115 6 41 gly glycosylation 978:990 arg1 alpha-fetoprotein alpha-fetoprotein PUBTATOR alpha-fetoprotein 174 However, because the presence of different glycoforms has been reported in alpha-fetoprotein preparations, both from human sources and from other species, it was important to establish the type and extent of glycosylation of alpha-fetoprotein prepared by our method. 16834341 6 27 part_of hFSH 872:875 arg1 Asn7 hFSH Asn7 OGER AminoAcid hFSH Asn7 For instance, except for one site in the beta subunit (Asn7) of hFSH all other sites in both species have sulfated glycoforms. 2033371 9 93 gly GalNAcOH 1987:1994 arg1 CD43 CD43 GalNAcOH PUBTATOR CD43 6693 Analyzing the six glycosyltransferases involved in the biosynthesis of these O-glycan structures it was found that in WAS lymphocytes high levels of beta 1----6 N-acetyl-glucosaminyl transferase are responsible for the expression of NeuNAc alpha 2----3Gal beta 1----3 (NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6) GalNAcOH on CD43. 7686446 6 32 gly AFP 1803:1805 arg1 fucosylation AFP fucosylation PUBTATOR AFP 174 Fucosylation of AFP produced in fetal liver increased in inverse proportion to the gestation period, in weeks, indicating that fucosylation of AFP in HCC may be related to the dedifferentiation of human hepatocytes through malignant transformation. 7686446 6 50 gly AFP 1676:1678 arg1 Fucosylation AFP Fucosylation PUBTATOR AFP 174 Fucosylation of AFP produced in fetal liver increased in inverse proportion to the gestation period, in weeks, indicating that fucosylation of AFP in HCC may be related to the dedifferentiation of human hepatocytes through malignant transformation. 7686446 6 92 gly fucosylation 1787:1798 arg1 AFP AFP PUBTATOR AFP 174 Fucosylation of AFP produced in fetal liver increased in inverse proportion to the gestation period, in weeks, indicating that fucosylation of AFP in HCC may be related to the dedifferentiation of human hepatocytes through malignant transformation. 7686446 6 106 gly Fucosylation 1660:1671 arg1 AFP AFP PUBTATOR AFP 174 Fucosylation of AFP produced in fetal liver increased in inverse proportion to the gestation period, in weeks, indicating that fucosylation of AFP in HCC may be related to the dedifferentiation of human hepatocytes through malignant transformation. 20624874 0 47 gly ADAMTS13 53:60 arg1 sugar chains ADAMTS13 sugar chains PUBTATOR ADAMTS13 11093 Proteolytic fragmentation and sugar chains of plasma ADAMTS13 purified by a conformation-dependent monoclonal antibody. 9136890 4 69 gly found 549:553 arg2 the native TfR AND the oligosaccharides the native TfR the oligosaccharides PUBTATOR TfR 7037 Human TfR isolated from placentae was used to characterize the structure of the oligosaccharides found in the native TfR. 8639592 0 97 gly inhibitor 92:100 arg1 carbohydrate structure tissue factor pathway inhibitor carbohydrate structure PUBTATOR tissue factor pathway inhibitor 7035 Amino acid sequence and carbohydrate structure of a recombinant human tissue factor pathway inhibitor expressed in Chinese hamster ovary cells: one N-and two O-linked carbohydrate chains are located between Kunitz domains 2 and 3 and one N-linked carbohydrate chain is in Kunitz domain 2. 10889209 4 55 gly glycosylated 655:666 arg1 GIRK1 GIRK1 PUBTATOR GIRK1 3760 Using chimeras between GIRK1 and GIRK4 as well as a GIRK1 N-glycosylation mutant, we report that GIRK1 was glycosylated at Asn(119), whereas GIRK4 was not glycosylated at Asn(132). 10889209 4 68 gly glycosylated 703:714 arg1 GIRK4 GIRK4 PUBTATOR GIRK4 3762 Using chimeras between GIRK1 and GIRK4 as well as a GIRK1 N-glycosylation mutant, we report that GIRK1 was glycosylated at Asn(119), whereas GIRK4 was not glycosylated at Asn(132). 11588155 1 79 gly B100 279:282 arg1 The carbohydrate composition apolipoprotein (apo) B100 The carbohydrate composition PUBTATOR apolipoprotein (apo) B100 338 The carbohydrate composition of apolipoprotein (apo) B100, particularly its degree of sialylation, may contribute to the atherogenic properties of low-density lipoprotein (LDL). 17015441 1 47 gly glycosylation 118:130 arg1 mouse TRPM8 mouse TRPM8 PUBTATOR TRPM8 Q7Z2W7 We have investigated the glycosylation, disulfide bonding, and subunit structure of mouse TRPM8. 12122212 7 38 gly glycosylation 1154:1166 arg1 CD21 CD21 PUBTATOR CD21 1380 We present evidence that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic glycosylation of CD21 would result in a more extended conformation, perhaps with additional epitopes for C3d binding. 2513186 0 45 gly activator 71:79 arg1 Carbohydrate structure tissue plasminogen activator Carbohydrate structure PUBTATOR tissue plasminogen activator P00750 Carbohydrate structure of recombinant human uterine tissue plasminogen activator expressed in mouse epithelial cells. 20624874 7 51 gly pADAMTS13 1477:1485 arg1 O-linked sugar chains pADAMTS13 O-linked sugar chains Cterm pADAMTS13 11093 Lectin blot analysis indicated the presence of non-reducing terminal α2-6 and α2-3-linked sialic acid residues with penultimate β-galactose residues on the N- and O-linked sugar chains of pADAMTS13, suggesting that pADAMTS13 is cleared from the circulation via the hepatic asialoglycoprotein receptor like other plasma glycoproteins. 20624874 7 51 gly pADAMTS13 1477:1485 arg1 N- pADAMTS13 N- Cterm pADAMTS13 11093 Lectin blot analysis indicated the presence of non-reducing terminal α2-6 and α2-3-linked sialic acid residues with penultimate β-galactose residues on the N- and O-linked sugar chains of pADAMTS13, suggesting that pADAMTS13 is cleared from the circulation via the hepatic asialoglycoprotein receptor like other plasma glycoproteins. 2059624 2 43 part_of r-HPg 502:506 arg1 Asn289 r-HPg Asn289 Cterm AminoAcid r-HPg P00747 Asn289 Previous studies from this laboratory have established that lepidopteran insect cells possess the glycosylation machinery needed to assemble N-linked complex-type oligosaccharides on Asn289 of recombinant human plasminogen (r-HPg). 12927778 8 38 gly glycoprotein 1140:1151 arg1 NPP-5 NPP-5 PUBTATOR NPP-5 316249 These data, therefore, suggest that NPP-5 is a neural oligomannosidic glycoprotein that may participate in neural cell communications. 23236605 2 34 gly contains 415:422 arg1 mouse AIM AND two N-glycans mouse AIM two N-glycans OGER AIM Q07108 Inactivation of N-glycosylation sites revealed that mouse AIM contains two N-glycans in the first and second scavenger receptor cysteine-rich domains, and that depletion of N-glycans decreased AIM secretion from producing cells. 11710528 7 15 gly hTR 956:958 arg1 the high mannose-type oligosaccharides hTR the high mannose-type oligosaccharides PUBTATOR hTR P25116 Our results demonstrate that the high mannose-type oligosaccharides of hTR ranged in size from Man5-R to Man9-R with cell-type specific patterns. 25187573 2 84 gly glycosylation 355:367 arg1 lubricin lubricin PUBTATOR lubricin 10216 Thus, a site-specific investigation of the glycosylation of lubricin was undertaken, in order to further understand the pathological mechanisms involved in these diseases. 17015441 4 29 gly glycosylated 573:584 arg1 Unmodified TRPM8 Unmodified TRPM8 PUBTATOR Unmodified TRPM8 Q7Z2W7 Unmodified TRPM8 migrates with an apparent mass of 129 kDa and can be glycosylated in Chinese hamster ovary cells to give glycoproteins with apparent masses of 136 and 147 kDa. 16847056 3 75 part_of beta2 411:415 arg1 Pro5 beta2 Pro5 Cterm AminoAcid beta2 Pro5 To determine whether beta2-linked sialic acids similarly impact Nav gating, we co-expressed beta2 with Nav1.5 or Nav1.2 in Pro5 (complete sialylation) and in Lec2 (essentially no sialylation) cells. 6118137 2 7 gly glycoprotein 147:158 arg1 the Thy-1 membrane glycoprotein the Thy-1 membrane glycoprotein OGER Thy-1 membrane glycoprotein P01830 The full sequence of the Thy-1 membrane glycoprotein of rat brain is reported. 23829323 7 49 gly glycoprotein 1317:1328 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 We use a human glycoprotein standard, haptoglobin, digested with trypsin and GluC, enriched for glycopeptides using HILIC chromatography, and analyzed by LC-MS/MS to demonstrate our algorithmic strategy and evaluate its performance. 12590919 4 5 gly N-glycosylation 680:694 arg1 hENT2 hENT2 PUBTATOR hENT2 3177 Our results show that although the apparent affinities for [3H]uridine and [3H]cytidine of the mutants were indistinguishable from those of the wild-type protein, N-glycosylation was required for efficient targeting of hENT2 to the plasma membrane. 12911312 0 63 gly O-glycosylation 44:58 arg1 full-length rat selenoprotein P full-length rat selenoprotein P PUBTATOR selenoprotein P 29360 Mass spectrometric identification of N- and O-glycosylation sites of full-length rat selenoprotein P and determination of selenide-sulfide and disulfide linkages in the shortest isoform. 19864504 9 35 gly ASA 1267:1269 arg1 the highest mannose-6-phosphate content ASA the highest mannose-6-phosphate content OGER ASA P15289 CHO cells cultured under bioreactor conditions yielded recombinant ASA with the most preserved N-glycan structures, the highest mannose-6-phosphate content and the highest similarity to non-recombinant enzyme. 19864504 9 35 gly ASA 1267:1269 arg1 the most preserved N-glycan structures ASA the most preserved N-glycan structures OGER ASA P15289 CHO cells cultured under bioreactor conditions yielded recombinant ASA with the most preserved N-glycan structures, the highest mannose-6-phosphate content and the highest similarity to non-recombinant enzyme. 22967898 4 33 gly N-glycosylated 471:484 arg1 cathepsin V cathepsin V PUBTATOR cathepsin V 1515 We demonstrated that cathepsin V is N-glycosylated at both Asn(221) and Asn(292) using mass spectrometry and site-directed mutagenesis. 21182469 6 17 part_of ABCC11 843:848 arg1 Gly180 ABCC11 Gly180 PUBTATOR AminoAcid ABCC11 85320 Gly180 On the other hand, the wild type (Gly180) of ABCC11 is associated with wet-type earwax, axillary osmidrosis, colostrum secretion from the mammary gland, and the potential susceptibility of breast cancer. 26467158 7 62 gly sites 1304:1308 arg1 BACE1 BACE1 sites PUBTATOR BACE1 23821 We purified BACE1 from Neuro2A cells and performed LC/ESI/MS analysis for BACE1-derived glycopeptides and mapped bisecting GlcNAc-modified sites on BACE1. 2390069 3 22 gly lactotransferrin 442:457 arg1 the glycans lactotransferrin the glycans PUBTATOR lactotransferrin 4057 A comparative analysis of the molar carbohydrate compositions of human leucocyte lactotransferrin and human milk lactotransferrin reveals that the glycans of leucocyte lactotransferrin differ essentially by the absence of fucose residues. 2390069 3 29 gly lactotransferrin 355:370 arg1 the molar carbohydrate compositions lactotransferrin the molar carbohydrate compositions PUBTATOR lactotransferrin 4057 A comparative analysis of the molar carbohydrate compositions of human leucocyte lactotransferrin and human milk lactotransferrin reveals that the glycans of leucocyte lactotransferrin differ essentially by the absence of fucose residues. 2390069 3 44 gly lactotransferrin 387:402 arg1 the molar carbohydrate compositions lactotransferrin the molar carbohydrate compositions PUBTATOR lactotransferrin 4057 A comparative analysis of the molar carbohydrate compositions of human leucocyte lactotransferrin and human milk lactotransferrin reveals that the glycans of leucocyte lactotransferrin differ essentially by the absence of fucose residues. 24884609 1 70 gly glycoprotein 167:178 arg1 Inter-alpha-trypsin inhibitor heavy chain H4 Inter-alpha-trypsin inhibitor heavy chain H4 PUBTATOR Inter-alpha-trypsin inhibitor heavy chain H4 3700 Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) is a 120 kDa acute-phase glycoprotein produced primarily in the liver, secreted into the blood, and identified in serum. 7541354 2 34 gly released 192:199 arg1 the vitronectin AND Oligosaccharides the vitronectin Oligosaccharides PUBTATOR vitronectin 7448 Oligosaccharides were released from the vitronectin by N-glycosidase F digestion and tagged with 2-aminopyridine; the pyridylamino-oligosaccharides were then fractionated by anion-exchange and reverse-phase HPLC. 17157876 1 91 gly glycoprotein 208:219 arg1 Human carboxypeptidase N (CPN) Human carboxypeptidase N (CPN) PUBTATOR CPN 1369 Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. 28327546 3 82 part_of FVIII 388:392 arg1 N582 FVIII N582 PUBTATOR SpecificSite FVIII 2157 N582 Here we characterize how hemophilia mutations near the unused N-glycosylation site of the A2 domain (N582) of FVIII affect protein conformation and intracellular trafficking. 29932112 4 17 gly N-glycosylation 547:561 arg1 Panx2 Panx2 PUBTATOR Panx2 56666 Our objectives were to validate the predicted N-glycosylation site of Panx2 and to study the effects of Panx2 glycosylation on localization and its capacity to interact with Panx1. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N98 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N90 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N45 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N78 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N90 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N45 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N78 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N45 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N78 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 The corticotropin-releasing factor (CRF) receptor type 1 AND N78 corticotropin-releasing factor (CRF) receptor type 1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite corticotropin-releasing factor (CRF) receptor type 1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N98 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N90 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N45 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N78 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N90 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N45 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N78 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N45 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N78 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 11595658 1 42 part_of contains 154:161 arg1 CRFR1 AND N78 CRFR1 N38, N45, N78, N90, and N98 PUBTATOR SpecificSite CRFR1 1394 N38, N45, N78, N90, and N98 The corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) contains five potential N-glycosylation sites: N38, N45, N78, N90, and N98. 18340083 10 66 gly N-glycosylation 1365:1379 arg1 GPIHBP1 GPIHBP1 PUBTATOR GPIHBP1 68453 These studies demonstrate that N-glycosylation of GPIHBP1 is important for the trafficking of GPIHBP1 to the cell surface. 9572875 7 38 gly nonglycosylated 1296:1310 arg1 nonglycosylated IGFBP-6 nonglycosylated IGFBP-6 PUBTATOR IGFBP-6 3489 Glycosylated IGFBP-6 exhibited greater resistance to proteolysis by chymotrypsin and trypsin than nonglycosylated IGFBP-6. 14693913 0 56 gly glycosylation 36:48 arg1 recombinant human bile salt-stimulated lipase recombinant human bile salt-stimulated lipase OGER bile salt-stimulated lipase P19835 Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris. 15687489 7 42 gly glycosylated 911:922 arg1 the glycosylated human pFGE the glycosylated human pFGE PUBTATOR pFGE 25870 We have crystallized the glycosylated human pFGE and determined its crystal structure at a resolution of 1.86 A. 11544325 0 30 gly glycosylations 9:22 arg1 human MD-2 human MD-2 PUBTATOR MD-2 23643 N-linked glycosylations at Asn(26) and Asn(114) of human MD-2 are required for toll-like receptor 4-mediated activation of NF-kappaB by lipopolysaccharide. 27313224 0 76 gly N559-glycan 17:27 arg1 the synaptic vesicle glycoprotein 2C synaptic vesicle glycoprotein 2C N559-glycan PUBTATOR synaptic vesicle glycoprotein 2C 22987 Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1. 27313224 0 82 gly glycoprotein 53:64 arg1 the synaptic vesicle glycoprotein 2C the synaptic vesicle glycoprotein 2C PUBTATOR synaptic vesicle glycoprotein 2C 22987 Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1. 1381905 5 77 gly deglycosylated 813:826 arg1 the deglycosylated SCF the deglycosylated SCF OGER SCF P21583 Structural analyses of the intact SCF, the deglycosylated SCF, and a deglycosylated C-terminal peptide were performed by laser desorption, fast atom bombardment, or electrospray mass spectrometry. 23027977 0 58 gly glycoprotein 45:56 arg1 Crystal structure Crystal structure PUBTATOR structure of Enpp1 5167 Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling. 6192908 0 12 gly alpha-fetoprotein 15:31 arg1 Sugar chain alpha-fetoprotein Sugar chain PUBTATOR alpha-fetoprotein 174 Sugar chain of alpha-fetoprotein produced in human yolk sac tumor. 2403553 14 34 gly contains 2096:2103 arg1 the human transferrin receptor AND O-linked oligosaccharides the human transferrin receptor O-linked oligosaccharides PUBTATOR transferrin receptor 7018 Thus, our results demonstrate that the human transferrin receptor contains O-linked oligosaccharides and that there are differences in the structures of both the O-linked and complex-type N-linked oligosaccharides on the receptors synthesized by different cell types. 16407218 5 4 part_of Asn-846 930:936 arg1 alpha2M alpha2M Asn-846 PUBTATOR SpecificSite alpha2M 2 Asn-846 We investigated the mechanism of formation of complexes between alpha2M and MBL and concluded that they form by the direct binding of oligomannose glycans Man(5-7) occupying Asn-846 on alpha2M to the lectin domains (carbohydrate recognition domains) of MBL. 6966283 11 11 gly inhibitor 1125:1133 arg1 The sialic acid alpha 1-protease inhibitor The sialic acid PUBTATOR alpha 1-protease inhibitor 5265 The sialic acid of human alpha 1-protease inhibitor was determined to be N-acetylneuraminic acid. 6966283 11 11 gly inhibitor 1125:1133 arg1 N-acetylneuraminic acid alpha 1-protease inhibitor N-acetylneuraminic acid PUBTATOR alpha 1-protease inhibitor 5265 The sialic acid of human alpha 1-protease inhibitor was determined to be N-acetylneuraminic acid. 3342257 1 1 gly linked 113:118 arg1 human kappa-casein AND The carbohydrate chains human kappa-casein The carbohydrate chains OGER kappa-casein P07498 The carbohydrate chains linked to human kappa-casein from mature milk were released by alkaline borohydride treatment as reduced oligosaccharides. 3342257 1 1 gly linked 113:118 arg1 human kappa-casein AND reduced oligosaccharides human kappa-casein reduced oligosaccharides OGER kappa-casein P07498 The carbohydrate chains linked to human kappa-casein from mature milk were released by alkaline borohydride treatment as reduced oligosaccharides. 24884609 9 6 part_of contained 1294:1302 arg1 recombinant ITIH4 AND Site N274 recombinant ITIH4 Site N274 high-mannose N-linked glycans PUBTATOR SpecificSite ITIH4 3700 N274 Site N274 contained high-mannose N-linked glycans in both serum and recombinant ITIH4. 11168369 6 60 gly O-glycosylated 1256:1269 arg1 albumin Kenitra albumin Kenitra PUBTATOR albumin Kenitra 213 Mass spectrometric analyses revealed that the four additional cysteine residues of the variant form two new S-S bridges and showed that albumin Kenitra is partially O-glycosylated by a monosialylated HexHexNAc structure. 12956774 8 64 gly N-glycosylation 1557:1571 arg1 DARC DARC PUBTATOR DARC 2532 We also showed that N-glycosylation of DARC occurred on N16SS and did not influence antibody and chemokine binding. 17322565 6 63 part_of EL 1066:1067 arg1 Asn-116 EL Asn-116 PUBTATOR SpecificSite EL 9388 Asn-116 Finally, we introduced Asn-116 of EL into the analogous positions within LPL and HL, resulting in N-linked glycosylation at this site. 27313224 11 19 gly SV2C-LD4 1839:1846 arg1 the complex N559-glycan SV2C the complex N559-glycan PUBTATOR SV2C 22987 In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding a third anchor point beside a ganglioside and the SV2C-LD4 peptide, for BoNT/A neuronal cell surface binding and uptake. 19343721 3 34 gly tg-PC 643:647 arg1 The N-glycans tg-PC The N-glycans Cterm tg-PC P02810 The N-glycans of tg-PC are complex sialylated structures, but less branched and partially sialylated. 19343721 3 34 gly tg-PC 643:647 arg1 complex sialylated structures tg-PC complex sialylated structures Cterm tg-PC P02810 The N-glycans of tg-PC are complex sialylated structures, but less branched and partially sialylated. 26620227 3 44 part_of proSP-B 418:424 arg1 Asn129 SP-B Asn129 OGER AminoAcid SP-B P07988 Asn129 The SNP causes an altered N-linked glycosylation modification at Asn129 of proSP-B, e.g. the C allele with this glycosylation site but not in the T allele. 27175940 5 43 part_of p27 987:989 arg1 Ser2 p27 Ser2 PUBTATOR AminoAcid p27 3429 Ser2 Cell proliferation was negatively regulated when Ser2 of p27 was replaced with Ala. 17117926 0 39 gly glycosylation 38:50 arg1 Wnt-5a Wnt-5a PUBTATOR Wnt-5a 7474 Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling. 27175940 2 8 gly glycosylation 451:463 arg1 p27 p27 PUBTATOR p27 3429 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 10842180 4 61 gly has 660:662 arg1 wild type (wt) Dpl AND two N-linked oligosaccharides wild type (wt) Dpl two N-linked oligosaccharides PUBTATOR Dpl 26434 Using recombinant Dpl expressed in Escherichia coli and mouse neuroblastoma cells we demonstrate that wild type (wt) Dpl, like PrP(C), adopts a predominantly alpha-helical conformation, forms intramolecular disulfide bonds, has two N-linked oligosaccharides, and is presented on the cell surface via a glycosylphosphatidylinositol anchor. 11696579 4 9 gly modification 752:763 arg3 eNOS AND O-linked N-acetylglucosamine modification eNOS O-linked N-acetylglucosamine modification OGER eNOS P29474 Hyperglycemia-associated inhibition of eNOS was accompanied by a twofold increase in O-linked N-acetylglucosamine modification of eNOS and a reciprocal decrease in O-linked serine phosphorylation at residue 1177. 11696579 4 86 gly eNOS 768:771 arg1 O-linked N-acetylglucosamine modification eNOS O-linked N-acetylglucosamine modification OGER eNOS P29474 Hyperglycemia-associated inhibition of eNOS was accompanied by a twofold increase in O-linked N-acetylglucosamine modification of eNOS and a reciprocal decrease in O-linked serine phosphorylation at residue 1177. 23661698 8 6 gly EphA2 1196:1200 arg1 the ligand's carbohydrates EphA2 the ligand's carbohydrates PUBTATOR EphA2 1969 Analysis of Eph/ephrin crystal structures reveals an interaction between the ligand's carbohydrates and two residues of EphA2: Asp-78 and Lys-136. 7682553 7 25 part_of bikunin 1152:1158 arg1 Ser10 bikunin Ser10 PUBTATOR AminoAcid bikunin 259 Ser10 Biochemical and mass spectrometric analysis of the peptides containing the cross-link indicate that it is mediated by a chondroitin-4-sulfate chain that originates from a typical O-glycosidic link to Ser10 of bikunin. 8034632 7 9 gly linkage 914:920 arg1 C-3 C-3 linkage PUBTATOR C-3 718 Sialylation may occur on the C-6 of the N-acetylgalactosamine involved in the carbohydrate-peptide linkage or on a terminal galactose residue, either on C-3 or on C-6. 8034632 7 9 gly linkage 914:920 arg1 C-6 C-6 linkage PUBTATOR C-6 729 Sialylation may occur on the C-6 of the N-acetylgalactosamine involved in the carbohydrate-peptide linkage or on a terminal galactose residue, either on C-3 or on C-6. 8286855 2 38 gly O-glycosylation 328:342 arg1 GpA GpA PUBTATOR GpA 2993 We have revised the sites of O-glycosylation in the extracellular domain of GpA by automated solid-phase Edman degradation, which allowed positive identification and quantitation of O-glycosylated Ser and Thr residues, as well as the single N-glycosylation site. 10756055 3 31 gly glycoprotein 736:747 arg1 Env Env PUBTATOR Env 100616444 We hypothesize that this alteration unmasks existing common extracellular structures reflecting a conserved three-dimensional similarity of important elements of CXCR4 and CCR5 that are involved in HIV envelope glycoprotein (Env) interaction. 10756055 3 31 gly glycoprotein 736:747 arg1 HIV envelope glycoprotein HIV envelope glycoprotein PUBTATOR HIV envelope glycoprotein 100616444 We hypothesize that this alteration unmasks existing common extracellular structures reflecting a conserved three-dimensional similarity of important elements of CXCR4 and CCR5 that are involved in HIV envelope glycoprotein (Env) interaction. 26993603 0 54 part_of Asn144 24:29 arg1 GP73 N-glycosylation GP73 N-glycosylation Asn144 PUBTATOR AminoAcid GP73 N-glycosylation 51280 Asn144 GP73 N-glycosylation at Asn144 reduces hepatocellular carcinoma cell motility and invasiveness. 21733844 8 49 gly glycosylated 1187:1198 arg1 hAQP10 hAQP10 PUBTATOR hAQP10 89872 Because only one third of hAQP10 was glycosylated yet the thermostability titration was mono-modal, we suggest that the presence of at least one glycosylated protein within each tetramer is sufficient to convey an enhanced structural stability to the remaining hAQP10 protomers of the tetramer. 20511397 1 7 gly glycoprotein 209:220 arg1 Apolipoprotein E Apolipoprotein E PUBTATOR Apolipoprotein E 348 Apolipoprotein E (apoE) is a 34-kDa glycoprotein secreted from various cells including hepatocytes and macrophages and plays an important role in remnant lipoprotein clearance, immune responses, Alzheimer disease, and atherosclerosis. 23376777 8 23 gly Deglycosylated 1486:1499 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 Deglycosylated forms of KCC4 also demonstrated decreased tumor formation and lung colonization in mouse xenografts. 17040911 4 41 gly glycosylated 754:765 arg1 recombinant mouse meprin A recombinant mouse meprin A OGER meprin A Nine of the ten potential N-linked glycosylation sites (Asn-41, Asn-152, Asn-234, Asn-270, Asn-330, Asn-426, Asn-452, Asn-546, and Asn-553) were found to be glycosylated in recombinant mouse meprin A using chemical and enzymatic deglycosylation methods and electrospray ionization mass spectrometry. 18988733 3 30 gly p65 548:550 arg1 O-GlcNAcylation NFkappaB p65 O-GlcNAcylation PUBTATOR NFkappaB p65 4790 In this work, we show that O-GlcNAcylation of NFkappaB p65 decreases binding to IkappaB alpha and increases transcriptional activity under hyperglycemic conditions. 17711303 0 82 gly N-Glycosylation 0:14 arg1 the human kappa opioid receptor the human kappa opioid receptor PUBTATOR kappa opioid receptor 4986 N-Glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway. 6587378 5 5 gly 2-glycoprotein 713:726 arg1 beta 2-glycoprotein I beta 2-glycoprotein I OGER beta 2-glycoprotein I P02749 This suggests that beta 2-glycoprotein I may have evolved by repeated duplications of a gene coding for a 60-amino acid segment of protein. 8349699 10 4 gly N-glycosylation 1444:1458 arg1 PGH synthase-1 PGH synthase-1 PUBTATOR PGH synthase-1 19224 Assuming that the N-glycosylation sites of PGH synthase-1 are on the luminal side of the endoplasmic reticulum (ER), and that the site of tryptic cleavage of ovine PGH synthase-1 (Arg277) is on the cytoplasmic side of the ER, we propose that both the NH2 and COOH termini of PGH synthase-1 are located in the lumen of the ER and that there are two transmembrane domains located between Asn144 and Arg277 and between Arg277 and Asn410, respectively. 21241660 0 27 gly O-glycosylated 16:29 arg1 PANP PANP PUBTATOR PANP 319352 PANP is a novel O-glycosylated PILRα ligand expressed in neural tissues. 6776528 9 74 part_of serine-126 1355:1364 arg1 the 511 alpha chain chain serine-126 OGER SpecificSite chain serine-126 The remainder of the 47A constant region, including the hinge region, is identical to the 511 alpha chain, except for two conservative changes in the CH1 domain: serine-126 and theonine-197 in the 511 alpha chain are both replaced by alanine in the 47A chain. 18340083 8 26 gly nonglycosylated 1126:1140 arg1 a nonglycosylated GPIHBP1 a nonglycosylated GPIHBP1 PUBTATOR GPIHBP1 68453 Consistent with this finding, cells expressing a nonglycosylated GPIHBP1 lack the ability to bind LPL or chylomicrons. 7774715 3 6 gly lactoferrin 380:390 arg1 Two N-linked glycans lactoferrin Two N-linked glycans OGER lactoferrin P02788 Two N-linked glycans from purified recombinant lactoferrin were released by hydrazinolysis and analyzed by 400-MHz 1H-NMR spectroscopy. 20356926 9 41 gly glycosylation 1147:1159 arg1 CREB-H CREB-H PUBTATOR CREB-H 84699 Taken together, our findings suggest that N-linked glycosylation is required for full activation of CREB-H through intramembrane proteolysis. 2001369 0 41 gly CD4 53:55 arg1 Carbohydrate structures CD4 Carbohydrate structures PUBTATOR CD4 920 Carbohydrate structures of recombinant soluble human CD4 expressed in Chinese hamster ovary cells. 6725284 0 12 gly galactoglycoprotein 56:74 arg1 galactoglycoprotein galactoglycoprotein PUBTATOR galactoglycoprotein 6693 The structure of the carbohydrate units of human plasma galactoglycoprotein determined by 500-megahertz 1H NMR spectroscopy. 26207632 4 52 gly O-glycosylation 643:657 arg1 the Obif protein the Obif protein OGER Obif protein Q8R138 RESULTS: First, we found that O-glycosylation of the Obif protein occurs at serine residue 36 in the Obif extracellular domain. 19556306 3 45 gly TLR4 599:602 arg1 complex type N-glycans TLR4 complex type N-glycans PUBTATOR TLR4 7099 Lectin blot and cell surface biotinylation revealed that TLR4 exhibited the 110 kDa protein with high mannose type N-glycans and the 130 kDa protein with complex type N-glycans and that only the 130 kDa TLR4 with complex type N-glycans was expressed on the cell surface. 19556306 3 70 gly protein 480:486 arg1 complex type N-glycans 110 kDa protein complex type N-glycans OGER 110 kDa protein Q9H1K0 Lectin blot and cell surface biotinylation revealed that TLR4 exhibited the 110 kDa protein with high mannose type N-glycans and the 130 kDa protein with complex type N-glycans and that only the 130 kDa TLR4 with complex type N-glycans was expressed on the cell surface. 19556306 3 70 gly protein 480:486 arg1 high mannose type N-glycans 110 kDa protein high mannose type N-glycans OGER 110 kDa protein Q9H1K0 Lectin blot and cell surface biotinylation revealed that TLR4 exhibited the 110 kDa protein with high mannose type N-glycans and the 130 kDa protein with complex type N-glycans and that only the 130 kDa TLR4 with complex type N-glycans was expressed on the cell surface. 7309709 5 26 gly prothrombin 645:655 arg1 the asparagine-linked sugar chains prothrombin the asparagine-linked sugar chains PUBTATOR prothrombin 2147 By the combination of sequential exoglycosidase digestion and methylation analysis, the structures of the asparagine-linked sugar chains of human prothrombin were confirmed to be as follows: (sequence in text). 11696579 7 29 part_of Akt 1496:1498 arg1 Ser1177 Akt Ser1177 PUBTATOR AminoAcid Akt 207 Ser1177 In contrast, when myc-tagged human eNOS carried a mutation at the Akt phosphorylation site (Ser1177), O-linked N-acetylglucosamine modification was unchanged by hyperglycemia and phospho-eNOS was undetectable. 10383441 4 35 part_of contains 571:578 arg1 murine DNase I AND Lys27 DNase I Lys27 and Lys74 PUBTATOR AminoAcid DNase I 13419 Lys27 and Lys74 We now demonstrate that murine DNase I, which contains Lys27 and Lys74, is phosphorylated only 20.9% when expressed in the same COS-1 cell system. 8172892 3 62 gly contained 487:495 arg1 AP beta AND galactosamine AP beta galactosamine PUBTATOR AP beta 6051 Amino acid analysis showed that AP alpha, but not AP beta and AP gamma, contained galactosamine in addition to glucosamine, thereby suggesting the presence of an O-linked sugar chain(s) in the molecule of AP alpha. 8172892 3 62 gly contained 487:495 arg1 AP alpha AND galactosamine AP alpha galactosamine PUBTATOR AP alpha 2028 Amino acid analysis showed that AP alpha, but not AP beta and AP gamma, contained galactosamine in addition to glucosamine, thereby suggesting the presence of an O-linked sugar chain(s) in the molecule of AP alpha. 10988252 4 59 gly sEGFR 622:626 arg1 the oligosaccharides EGFR the oligosaccharides PUBTATOR EGFR 1956 After liberation of the oligosaccharides from sEGFR with PNGase F, the glycans were fractionated along different routes, including Concanavalin A affinity chromatography, anion-exchange chromatography, HPLC and high-pH anion-exchange chromatography. 21908432 6 20 part_of GPIbβ 822:826 arg1 residue Tyr106 GPIbβ, residue Tyr106 PUBTATOR AminoAcid GPIbβ, 2812 residue Tyr106 Central to this interface is residue Tyr106 from GPIbβ, which inserts into a pocket generated by 2 loops (b,c) from GPIX. 6619128 0 19 gly IgD 68:70 arg1 the O-glycosidically linked oligosaccharides IgD the O-glycosidically linked oligosaccharides OGER IgD P01880 Structures of the O-glycosidically linked oligosaccharides of human IgD. 14970177 4 16 gly O-glycosylated 816:829 arg1 hIL-6 hIL-6 OGER hIL-6 P05231 Although hIL-6 is also N-glycosylated at N73 and multiply O-glycosylated, neither N-linked nor O-linked glycosylation is necessary for IL-6 receptor alpha-dependent binding to gp130 or signaling through JAK1-STAT1/3. 14970177 4 28 gly N-glycosylated 781:794 arg1 hIL-6 hIL-6 OGER hIL-6 P05231 Although hIL-6 is also N-glycosylated at N73 and multiply O-glycosylated, neither N-linked nor O-linked glycosylation is necessary for IL-6 receptor alpha-dependent binding to gp130 or signaling through JAK1-STAT1/3. 17715132 9 40 gly N-glycosylation 1149:1163 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We propose that N-glycosylation of Pannexin1 could be a significant mechanism for regulating the trafficking of these membrane proteins to the cell surface in different tissues. 22399287 10 18 gly glycosylated 1319:1330 arg1 CNNM2 CNNM2 PUBTATOR CNNM2 54805 Mutagenesis screening showed that CNNM2 is glycosylated at residue Asn-112, stabilizing CNNM2 on the plasma membrane. 17563389 8 50 gly glycosylation 1502:1514 arg1 NK1R NK1R PUBTATOR NK1R 6869 Therefore, glycosylation of NK1R may stabilize the receptor in the plasma membrane. 8323299 0 39 gly glycoproteins 94:106 arg1 lamp-2 lamp-2 PUBTATOR lamp-2 3920 Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2. 8323299 0 39 gly glycoproteins 94:106 arg1 lamp-1 lamp-1 PUBTATOR lamp-1 3916 Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2. 2275556 7 109 gly glycosylation 1427:1439 arg1 mouse cathepsin L mouse cathepsin L PUBTATOR cathepsin L 13039 Site-directed mutagenesis of the mouse liver cDNA and expression in COS monkey cells was used to examine the glycosylation of mouse cathepsin L. 8033893 0 35 gly glycosylated 22:33 arg1 platelet factor 4 platelet factor 4 OGER platelet factor 4 P06765 Rat platelets contain glycosylated and non-glycosylated forms of platelet factor 4. 8033893 0 68 gly non-glycosylated 39:54 arg1 platelet factor 4 platelet factor 4 OGER platelet factor 4 P06765 Rat platelets contain glycosylated and non-glycosylated forms of platelet factor 4. 8344278 1 15 gly A 242:242 arg1 the oligosaccharide moieties saposin A the oligosaccharide moieties Cterm saposin A We have determined and compared the structures of the oligosaccharide moieties of saposin A, C and D purified from the spleen of a patient with Gaucher disease. 2403553 8 61 gly contains 1028:1035 arg1 The transferrin receptor AND O-linked oligosaccharides The transferrin receptor O-linked oligosaccharides PUBTATOR transferrin receptor 7018 The transferrin receptor from all three cell types contains O-linked oligosaccharides that are released from peptide by mild base/borohydride treatment. 2415652 7 80 gly oligosaccharides 1364:1379 arg1 hCG beta hCG beta oligosaccharides PUBTATOR hCG beta 1082 In contrast, over 50% of the O-linked oligosaccharides in hCG beta from the JAr choriocarcinoma cell line are hexasaccharides. 2415652 7 117 gly beta 1388:1391 arg1 hexasaccharides hCG beta hexasaccharides PUBTATOR hCG beta 1082 In contrast, over 50% of the O-linked oligosaccharides in hCG beta from the JAr choriocarcinoma cell line are hexasaccharides. 2415652 7 117 gly beta 1388:1391 arg1 the O-linked oligosaccharides hCG beta the O-linked oligosaccharides PUBTATOR hCG beta 1082 In contrast, over 50% of the O-linked oligosaccharides in hCG beta from the JAr choriocarcinoma cell line are hexasaccharides. 26993603 3 7 part_of GP73 560:563 arg1 Asn144 GP73 Asn109, Asn144 and Asn398 PUBTATOR AminoAcid GP73 51280 Asn109, Asn144 and Asn398 We identified three GP73 N-glycosylation sites: Asn109, Asn144 and Asn398. 26993603 3 7 part_of GP73 560:563 arg1 Asn109 GP73 Asn109, Asn144 and Asn398 PUBTATOR AminoAcid GP73 51280 Asn109, Asn144 and Asn398 We identified three GP73 N-glycosylation sites: Asn109, Asn144 and Asn398. 26993603 3 7 part_of GP73 560:563 arg1 Asn109 GP73 Asn109, Asn144 and Asn398 PUBTATOR AminoAcid GP73 51280 Asn109, Asn144 and Asn398 We identified three GP73 N-glycosylation sites: Asn109, Asn144 and Asn398. 8639509 0 72 gly B-crystallin 62:73 arg1 Dynamic O-GlcNAcylation alpha B-crystallin Dynamic O-GlcNAcylation OGER alpha B-crystallin P02511 Dynamic O-GlcNAcylation of the small heat shock protein alpha B-crystallin. 25802287 13 74 gly glycans 2170:2176 arg1 Asn 563 Asn 563 Asn 563 SpecificSite Asn 563, Asn 402 Besides complex type glycans on Asn 395, 332, 171, and on the J chain, we observed oligomannosidic glycans on Asn 563, Asn 402 and minor amounts of oligomannosidic glycans on the glycosite Asn 171. 25802287 13 74 gly glycans 2170:2176 arg1 Asn 402 Asn 402 Asn 402 SpecificSite Asn 563, Asn 402 Besides complex type glycans on Asn 395, 332, 171, and on the J chain, we observed oligomannosidic glycans on Asn 563, Asn 402 and minor amounts of oligomannosidic glycans on the glycosite Asn 171. 25802287 13 74 gly glycans 2170:2176 arg1 the glycosite Asn 171 the glycosite Asn 171 the glycosite Asn 171 SpecificSite glycosite Asn 171 Besides complex type glycans on Asn 395, 332, 171, and on the J chain, we observed oligomannosidic glycans on Asn 563, Asn 402 and minor amounts of oligomannosidic glycans on the glycosite Asn 171. 25802287 13 99 gly glycans 2092:2098 arg1 Asn 395, 332 Asn 395, 332 Asn 395, 332 SpecificSite Asn 395, 332, 171 Besides complex type glycans on Asn 395, 332, 171, and on the J chain, we observed oligomannosidic glycans on Asn 563, Asn 402 and minor amounts of oligomannosidic glycans on the glycosite Asn 171. 12731890 0 57 gly glycosylation 20:32 arg1 the epidermal growth factor receptor the epidermal growth factor receptor PUBTATOR epidermal growth factor receptor 1956 Characterization of glycosylation sites of the epidermal growth factor receptor. 7107587 2 85 gly fibrinogen 149:158 arg1 The carbohydrate composition fibrinogen The carbohydrate composition PUBTATOR fibrinogen 2244 The carbohydrate composition of fibrinogen and constituent S-carboxymethylated chains was determined. 10814696 0 30 gly A 70:70 arg1 High-mannose-type oligosaccharides arylsulfatase A High-mannose-type oligosaccharides PUBTATOR arylsulfatase A 410 High-mannose-type oligosaccharides from human placental arylsulfatase A are core fucosylated as confirmed by MALDI MS. Despite numerous studies on arylsulfatase A, the structure of its glycans is not well understood. 8174273 4 32 gly glycoprotein 1234:1245 arg1 alpha 1-antitrypsin alpha 1-antitrypsin PUBTATOR alpha 1-antitrypsin 5265 Further, the fucosylated alteration of the sugar chain was detected also in alpha 1-antitrypsin, hemopexin, alpha 1-acid glycoprotein and alpha 2-HS glycoprotein from one of the patients with increased fucosylated transferrin. 8174273 4 56 gly glycoprotein 1262:1273 arg1 alpha 2-HS glycoprotein alpha 2-HS glycoprotein PUBTATOR alpha 2-HS glycoprotein 197 Further, the fucosylated alteration of the sugar chain was detected also in alpha 1-antitrypsin, hemopexin, alpha 1-acid glycoprotein and alpha 2-HS glycoprotein from one of the patients with increased fucosylated transferrin. 8174273 4 56 gly glycoprotein 1262:1273 arg1 alpha 1-antitrypsin alpha 1-antitrypsin PUBTATOR alpha 1-antitrypsin 5265 Further, the fucosylated alteration of the sugar chain was detected also in alpha 1-antitrypsin, hemopexin, alpha 1-acid glycoprotein and alpha 2-HS glycoprotein from one of the patients with increased fucosylated transferrin. 8174273 4 72 gly fucosylated 1315:1325 arg1 increased fucosylated transferrin increased fucosylated transferrin PUBTATOR transferrin 7018 Further, the fucosylated alteration of the sugar chain was detected also in alpha 1-antitrypsin, hemopexin, alpha 1-acid glycoprotein and alpha 2-HS glycoprotein from one of the patients with increased fucosylated transferrin. 20147294 7 69 gly glycosylation 906:918 arg1 ADA2 ADA2 PUBTATOR ADA2 51816 An extensive glycosylation and the presence of a conserved disulfide bond and a signal peptide in ADA2 strongly suggest that ADA2, in contrast to ADA1, is specifically designed to act in the extracellular environment. 14711516 6 6 part_of Thy-1 1015:1019 arg1 asparagine 60 Recombinant Thy-1 asparagine 60 PUBTATOR SpecificSite Recombinant Thy-1 P04216 asparagine 60 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 7068558 4 19 gly lactoferrin 775:785 arg1 the sugar chain moiety lactoferrin the sugar chain moiety OGER lactoferrin P02788 A structural study of the oligosaccharides obtained from the two samples by sequential exoglycosidase digestion in combination with methylation analysis also gave the same results indicating that there is no racial difference both in quality and quantity of the sugar chain moiety of lactoferrin. 15331613 1 48 gly glycosylation 496:508 arg1 NGC NGC PUBTATOR NGC 29873 NGC dramatically changed its structure from a proteoglycan to a nonproteoglycan form with cerebellar development, whereas a small portion of NGC molecules existed in a nonproteoglycan form in the other areas of the mature CNS, suggesting that the CS glycosylation of NGC is developmentally regulated in the whole CNS. 22688517 4 43 gly N-glycosylation 470:484 arg1 recombinant human LOX-1 recombinant human LOX-1 PUBTATOR LOX-1 4973 The present study was aimed at elucidating the N-glycosylation of recombinant human LOX-1 with regard to N-glycan profile and N-glycosylation sites. 1694784 8 24 gly HC 1098:1099 arg1 the carbohydrate composition protein HC the carbohydrate composition PUBTATOR protein HC 259 No difference was found between the carbohydrate composition of urinary and plasma protein HC. 26828122 4 13 gly glycoprotein 953:964 arg1 the hTPPT protein the hTPPT protein PUBTATOR hTPPT protein 80736 Using several approaches including inhibiting de novo N-glycosylation in human colonic epithelial NCM460 cells with tunicamycin as well as enzymatic de-glycosylation, we show that the hTPPT protein is, indeed, a glycoprotein. 12435857 8 105 part_of THTR-1 1597:1602 arg1 the THTR-1 consensus N-glycosylation acceptor asparagine 63 THTR-1 the THTR-1 consensus N-glycosylation acceptor asparagine 63 PUBTATOR SpecificSite THTR-1 10560 asparagine 63 Consistently, either treatment with tunicamycin or substitution of the THTR-1 consensus N-glycosylation acceptor asparagine 63 with glutamine, abolished its glycosylation and plasma membrane targeting. 7841792 4 18 gly glycopeptide 653:664 arg1 a mutant human albumin Casebrook a mutant human albumin Casebrook OGER albumin Casebrook P02768 Using a model glycopeptide from a mutant human albumin Casebrook, glycosylated PTH-Asn was recovered after sequential solid-phase Edman degradation, subjected to acid hydrolysis and the sugars were identified by high performance anion exchange chromatography with pulsed amperometric detection. 7841792 4 51 gly glycosylated 705:716 arg1 glycosylated PTH-Asn glycosylated PTH-Asn PUBTATOR AminoAcid PTH 5741 Using a model glycopeptide from a mutant human albumin Casebrook, glycosylated PTH-Asn was recovered after sequential solid-phase Edman degradation, subjected to acid hydrolysis and the sugars were identified by high performance anion exchange chromatography with pulsed amperometric detection. 23829323 0 63 gly microheterogeneity 40:57 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Exploring site-specific N-glycosylation microheterogeneity of haptoglobin using glycopeptide CID tandem mass spectra and glycan database search. 23829323 0 104 gly N-glycosylation 24:38 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Exploring site-specific N-glycosylation microheterogeneity of haptoglobin using glycopeptide CID tandem mass spectra and glycan database search. 1323463 0 43 gly mucin 143:147 arg1 the acidic O-linked carbohydrate chains mucin the acidic O-linked carbohydrate chains PUBTATOR mucin 281333 Characterisation by mass spectrometry and 1H-NMR of novel hexasaccharides among the acidic O-linked carbohydrate chains of bovine submaxillary mucin. 7592613 0 31 gly glycoprotein 77:88 arg1 glycodelin glycodelin PUBTATOR glycodelin 5047 Structural analysis of the oligosaccharides derived from glycodelin, a human glycoprotein with potent immunosuppressive and contraceptive activities. 15522226 2 47 gly glycoprotein 243:254 arg1 The WFS1 protein The WFS1 protein PUBTATOR WFS1 protein 22393 The WFS1 protein is a glycoprotein located in the endoplasmic reticulum (ER) membrane but its function is poorly understood. 8349699 9 31 gly glycosylation 1351:1363 arg1 PGH synthase-2 PGH synthase-2 PUBTATOR PGH synthase-2 19225 Glycosylation of PGH synthase-2 is necessary for expression of enzyme activity, but glycosylation of PGH synthase-2 at Asn580 per se does not affect activity. 8349699 9 70 gly Glycosylation 1267:1279 arg1 PGH synthase-2 PGH synthase-2 PUBTATOR PGH synthase-2 19225 Glycosylation of PGH synthase-2 is necessary for expression of enzyme activity, but glycosylation of PGH synthase-2 at Asn580 per se does not affect activity. 28531887 11 35 gly N-glycosylation 1425:1439 arg1 MT4-MMP MT4-MMP PUBTATOR MT4-MMP 4326 Moreover, we identified Asn318 as the single N-glycosylation site of MT4-MMP. 29671580 5 30 gly α-1B-glycoprotein 986:1002 arg1 α-1B-glycoprotein α-1B-glycoprotein PUBTATOR -1B-glycoprotein 1 In addition, two novel atypical glycosites (with N-X-V motif) were identified and validated from albumin and α-1B-glycoprotein. 2386787 3 18 gly glycosylation 895:907 arg1 the insect cell expressed recombinant human plasminogen the insect cell expressed recombinant human plasminogen OGER plasminogen P00747 The glycosylation pattern of the insect cell expressed recombinant human plasminogen showed considerable microheterogeneity, with identifiable high-mannose carbohydrate (Man9GlcNAc2) and truncated high-mannose oligosaccharide (Man5GlcNAc2, Man4GlcNAc2, and Man3GlcNAc2). 10712595 3 110 part_of antithrombin 473:484 arg1 Asn135 antithrombin Asn135 PUBTATOR AminoAcid antithrombin 462 Asn135 Due to a single nucleotide replacement, Asn135 of the antithrombin in higher vertebrates is substituted by Asp in the salmon homolog. 18340083 3 14 gly N-glycosylated 505:518 arg1 mouse GPIHBP1 mouse GPIHBP1 PUBTATOR GPIHBP1 68453 Here, we show that mouse GPIHBP1 is N-glycosylated at Asn-76 within the Ly-6 domain. 18787108 0 15 gly glycoprotein 63:74 arg1 CTRP9 CTRP9 PUBTATOR CTRP9 239126 Identification and characterization of CTRP9, a novel secreted glycoprotein, from adipose tissue that reduces serum glucose in mice and forms heterotrimers with adiponectin. 7511099 3 44 gly AFP 946:948 arg1 the terminal galactose Desialylated AFP the terminal galactose PUBTATOR Desialylated AFP 174 Desialylated AFP with the terminal galactose of the Man alpha 1-->6 antenna with or without the galactose of the other arm also had a migration of AFP-P4, and other hydrolytic intermediates without the terminal galactose of the Man alpha 1-->6 arm with and without the galactose of the other antenna had mobilities of AFP-P3s and AFP-P3, respectively. 7511099 3 63 gly Desialylated 933:944 arg1 Desialylated AFP Desialylated AFP PUBTATOR Desialylated AFP 174 Desialylated AFP with the terminal galactose of the Man alpha 1-->6 antenna with or without the galactose of the other arm also had a migration of AFP-P4, and other hydrolytic intermediates without the terminal galactose of the Man alpha 1-->6 arm with and without the galactose of the other antenna had mobilities of AFP-P3s and AFP-P3, respectively. 22187327 3 17 gly ICAM-5 519:524 arg1 the N-glycans ICAM-5 the N-glycans PUBTATOR ICAM-5 15898 Here, we produced fifteen ICAM-5 gene constructs, in which each potential N-glycosylation site was mutated, to elucidate the function of the N-glycans of ICAM-5, and observed the effects of transfection of them on a neuronal cell line, Neuro-2a (N2a). 8627329 0 2 gly oligosaccharide 9:23 arg1 the 75-kDa neurotrophin receptor neurotrophin receptor oligosaccharide PUBTATOR neurotrophin receptor 4923 O-linked oligosaccharide on the 75-kDa neurotrophin receptor. 19951703 8 52 gly deglycosylated 1054:1067 arg1 the deglycosylated UGT1A9 the deglycosylated UGT1A9 PUBTATOR UGT1A9 54600 To the contrary, the deglycosylated UGT1A9, which was produced by the treatment with Endo H under the non-denaturing condition, showed the same enzyme kinetics as the control. 20427285 6 1 part_of ZPI 826:828 arg1 Glu-383 ZPI residues Glu-313 and Glu-383 PUBTATOR SpecificSite ZPI 51156 residues Glu-313 and Glu-383 Modeling of the ternary Michaelis complex implicated ZPI residues Glu-313 and Glu-383 in fXa binding. 20427285 6 1 part_of ZPI 826:828 arg1 Glu-313 ZPI residues Glu-313 and Glu-383 PUBTATOR SpecificSite ZPI 51156 residues Glu-313 and Glu-383 Modeling of the ternary Michaelis complex implicated ZPI residues Glu-313 and Glu-383 in fXa binding. 20427285 6 1 part_of ZPI 826:828 arg1 Glu-313 ZPI residues Glu-313 and Glu-383 PUBTATOR SpecificSite ZPI 51156 residues Glu-313 and Glu-383 Modeling of the ternary Michaelis complex implicated ZPI residues Glu-313 and Glu-383 in fXa binding. 1900431 5 13 gly Fucosylation 599:610 arg1 t-PA t-PA PUBTATOR t-PA 5327 Fucosylation of threonine-61 was observed in t-PA isolated from the Bowes melanoma cell line and from recombinant expression systems using Chinese hamster ovary or human embryonic kidney cells. 1900431 5 32 gly observed 632:639 arg2 t-PA AND Fucosylation t-PA Fucosylation PUBTATOR t-PA 5327 Fucosylation of threonine-61 was observed in t-PA isolated from the Bowes melanoma cell line and from recombinant expression systems using Chinese hamster ovary or human embryonic kidney cells. 19571171 1 55 gly Golgi-resident 152:165 arg1 N-Acetylglucosamine-6-sulfotransferase-1 Golgi-resident N-Acetylglucosamine-6-sulfotransferase-1 Cterm Golgi-resident N-Acetylglucosamine-6-sulfotransferase-1 (GlcNAc6ST-1) is a Golgi-resident glycoprotein that is responsible for sulfation of the l-selectin ligand on endothelial cells. 14699159 0 90 gly Underglycosylation 0:17 arg1 ATF6 ATF6 PUBTATOR ATF6 22926 Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response. 7462199 0 43 gly globulin 57:64 arg1 the sugar chains cold-insoluble globulin the sugar chains PUBTATOR cold-insoluble globulin 2335 Structural studies of the sugar chains of cold-insoluble globulin isolated from human plasma. 22363519 4 22 gly Toll-like 582:590 arg1 a model repeat protein Toll-like receptor4 a model repeat protein PUBTATOR Toll-like receptor4 7099 As a model repeat protein, a Toll-like receptor4 (TLR4) decoy receptor composed of leucine-rich repeat (LRR) modules was used, and its interaction interface was rationally engineered to increase the binding affinity for myeloid differentiation protein 2 (MD2). 22363519 4 71 gly TLR4 603:606 arg1 a model repeat protein TLR4 a model repeat protein PUBTATOR TLR4 7099 As a model repeat protein, a Toll-like receptor4 (TLR4) decoy receptor composed of leucine-rich repeat (LRR) modules was used, and its interaction interface was rationally engineered to increase the binding affinity for myeloid differentiation protein 2 (MD2). 22363519 4 80 gly receptor4 592:600 arg1 a model repeat protein Toll-like receptor4 a model repeat protein PUBTATOR Toll-like receptor4 7099 As a model repeat protein, a Toll-like receptor4 (TLR4) decoy receptor composed of leucine-rich repeat (LRR) modules was used, and its interaction interface was rationally engineered to increase the binding affinity for myeloid differentiation protein 2 (MD2). 6177036 0 18 gly glycoprotein 20:31 arg1 Neuronal cell Thy-1 glycoprotein Neuronal cell Thy-1 glycoprotein PUBTATOR Thy-1 glycoprotein 21838 Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. 9343410 4 12 gly contain 790:796 arg1 Sp1 AND a dominant O-GlcNAc residue Sp1 a dominant O-GlcNAc residue OGER Sp1 Q8N907 When the model Sp1 peptide was overexpressed in primate cells, this 97-amino-acid domain of Sp1 was found to contain a dominant O-GlcNAc residue at high stoichiometry, which allowed the mapping and mutagenesis of this glycosylation site. 19592704 5 3 part_of NCEH 851:854 arg1 Asn NCEH Asn(270), Asn(367), and Asn(389) PUBTATOR SpecificSite NCEH 57552 Asn(270), Asn(367), and Asn(389) All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 19592704 5 3 part_of NCEH 851:854 arg1 Asn NCEH Asn(270), Asn(367), and Asn(389) PUBTATOR SpecificSite NCEH 57552 Asn(270), Asn(367), and Asn(389) All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 19592704 5 3 part_of NCEH 851:854 arg1 Asn NCEH Asn(270), Asn(367), and Asn(389) PUBTATOR SpecificSite NCEH 57552 Asn(270), Asn(367), and Asn(389) All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 17925379 3 14 gly glycosylated 693:704 arg1 Panx3 Panx3 PUBTATOR Panx3 116337 Furthermore, N-glycosidase treatment showed that both Panx1 (approximately 41-48 kD species) and Panx3 (approximately 43 kD) were glycosylated, whereas N-linked glycosylation-defective mutants exhibited a decreased ability to be transported to the cell surface. 17925379 3 14 gly glycosylated 693:704 arg1 Panx1 Panx1 PUBTATOR Panx1 24145 Furthermore, N-glycosidase treatment showed that both Panx1 (approximately 41-48 kD species) and Panx3 (approximately 43 kD) were glycosylated, whereas N-linked glycosylation-defective mutants exhibited a decreased ability to be transported to the cell surface. 23395175 6 29 gly O-GlcNAcylation 842:856 arg1 PER2 PER2 O-GlcNAcylation PUBTATOR PER2 8864 In addition, O-GlcNAcylation of a region in PER2 known to regulate human sleep phase (S662-S674) competes with phosphorylation of this region, and this interplay is at least partly mediated by glucose levels. 1898343 0 30 gly elastase 43:50 arg1 Carbohydrate structure pancreatic elastase 1 Carbohydrate structure PUBTATOR pancreatic elastase 1 1990 Carbohydrate structure of human pancreatic elastase 1. 20332087 3 30 gly MPO 470:472 arg1 the N-glycan composition MPO the N-glycan composition PUBTATOR MPO 4353 Here, the N-glycan composition of native dimeric human MPO purified from neutrophils and of monomeric MPO recombinantly expressed in Chinese hamster ovary cells has been investigated. 20332087 3 48 gly MPO 517:519 arg1 the N-glycan composition MPO the N-glycan composition PUBTATOR MPO 4353 Here, the N-glycan composition of native dimeric human MPO purified from neutrophils and of monomeric MPO recombinantly expressed in Chinese hamster ovary cells has been investigated. 1694179 12 37 gly glycosylated 1694:1705 arg1 protein C protein C Cterm protein C The percentage of protein C that is glycosylated at this site may therefore depend at least in part on the rate of disulfide bond formation which may in turn be related to the rate of protein synthesis. 19038967 5 76 gly deglycosylated 1016:1029 arg1 deglycosylated pro-TPP1 deglycosylated pro-TPP1 Cterm AminoAcid TPP1 The crystal structure of deglycosylated pro-TPP1 was determined at 1.85 angstroms resolution. 2493268 0 46 gly glycosylated 37:48 arg1 human pancreatic stone protein human pancreatic stone protein OGER pancreatic stone protein P05451 N-terminal sequence extension in the glycosylated forms of human pancreatic stone protein. 8174273 1 33 gly chains 138:143 arg1 serum transferrin transferrin chains PUBTATOR transferrin 7018 The asparagine-linked sugar chains in serum transferrin purified from patients with hepatocellular carcinoma (n = 13), healthy individuals (n = 5) and patients with liver cirrhosis (n = 6) were compared. 19467646 9 6 gly glycosylated 1172:1183 arg1 BDNF BDNF PUBTATOR BDNF 627 Treatment with N-glycanase and plasmin reduced the size of the higher molecular weight bands, confirming the glycosylated pro-form of BDNF. 7524900 2 15 gly contains 328:335 arg1 proMBP AND O-linked glycan proMBP O-linked glycan PUBTATOR proMBP 5553 It is shown that proMBP contains O-linked glycan bound to Ser-24, Thr-25 (fully substituted), and to Thr-23 and Thr-34 (partially substituted). 11080501 0 106 gly N-glycosylation 48:62 arg1 the human gastrointestinal carcinoma antigen GA733-2 the human gastrointestinal carcinoma antigen GA733-2 OGER GA733-2 P16422 Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM). 11676606 6 69 gly structures 768:777 arg1 sCD154 CD154 structures PUBTATOR CD154 959 Detailed carbohydrate analysis revealed high-mannose structures on sCD154 purified from Pichia pastoris, whereas CD154 purified from Chinese hamster ovary E1A contained heterogeneous populations of complex carbohydrates. 23851396 1 5 gly glycoproteins 145:157 arg1 Folate receptors Folate receptors PUBTATOR Folate receptors (FRα, FRβ and FRγ) 2348 Folate receptors (FRα, FRβ and FRγ) are cysteine-rich cell-surface glycoproteins that bind folate with high affinity to mediate cellular uptake of folate. 2514791 4 46 gly N-glycosylated 853:866 arg1 type II t-PA type II t-PA PUBTATOR t-PA 5327 Our results suggest the following: (i) type I and type II t-PA are N-glycosylated in an identical way at Asn-117 and Asn-448, when isolated from the same cell line; (ii) Asn-117 is predominantly associated with oligomannose-type structures in all cases; (iii) Asn-184 and Asn-448 are predominantly associated with complex-type structures when t-PA is isolated from fibroblast cells, but with both complex- and oligomannose-type structures when isolated from melanoma cells; (iv) fibroblast cell derived t-PA is associated with both neutral and sialylated oligosaccharides, while melanoma cell derived t-PA is also associated with sulfated oligosaccharides, which are located exclusively at Asn-448 of type II t-PA; (v) no complex-type structures occur in common between t-PA from the two cell lines. 15454184 7 41 gly rhFS 1014:1017 arg1 the oligosaccharides rhFS the oligosaccharides Cterm rhFS This finding was supported by mass spectrometric oligosaccharide profiling, in which the m/z values and elution times of some of the oligosaccharides from rhFS were in good agreement with those of standard oligosaccharides. 16877748 4 45 gly carries 662:668 arg1 sICAM-1 AND complex-type N-glycans sICAM-1 complex-type N-glycans Cterm sICAM-1 3383 MIP-2 induction is glycosylation dependent, as it is strongly enhanced when sICAM-1 carries sialylated, complex-type N-glycans as synthesized by wild-type Chinese hamster ovary (CHO) cells. 2363690 5 29 gly present 976:982 arg1 IgG2 AND Unusual monoantennary oligosaccharides IgG2 Unusual monoantennary oligosaccharides OGER IgG2 P01859 Unusual monoantennary oligosaccharides present in IgG2 and IgG3 proteins were isolated and their structures determined. 2363690 5 29 gly present 976:982 arg1 IgG3 AND Unusual monoantennary oligosaccharides IgG3 Unusual monoantennary oligosaccharides PUBTATOR IgG3 proteins P01860 Unusual monoantennary oligosaccharides present in IgG2 and IgG3 proteins were isolated and their structures determined. 10383441 1 9 gly glycoprotein 162:173 arg1 bovine DNase I bovine DNase I PUBTATOR DNase I 282217 We have reported that bovine DNase I, a secretory glycoprotein, acquires mannose 6-phosphate residues on 12.6% of its Asn-linked oligosaccharides when expressed in COS-1 cells and that the extent of phosphorylation increases to 79.2% when lysines are placed at positions 27 and 74 of the mature protein (Nishikawa, A., Gregory, W. , Frenz, J., Cacia, J., and Kornfeld, S. (1997) J. Biol. 11904304 4 51 gly -glycosylated 876:888 arg1 c-Myc c-Myc PUBTATOR c-Myc 4609 Using these antibodies together with a commercial anti-Thr-58-phosphorylated c-Myc antibody, we simultaneously detected three forms of c-Myc (Thr-58-unmodified, -phosphorylated, and -glycosylated). 20511397 7 32 gly sialylated 1385:1394 arg1 cell-derived apoE cell-derived apoE PUBTATOR apoE 348 Comparison of plasma and cellular/secreted apoE from the same donor confirmed that cell-derived apoE is more extensively sialylated than plasma apoE. 22967898 5 34 gly N-glycosylation 571:585 arg1 cathepsin V cathepsin V PUBTATOR cathepsin V 1515 N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells. 18490449 6 1 gly contained 1089:1097 arg1 myelin Opalin AND O-glycans myelin Opalin O-glycans PUBTATOR Opalin 226115 Enzymatic deglycosylation showed that myelin Opalin contained N- and O-glycans, and that the O-glycans, at least, had negatively charged sialic acids. 18490449 6 1 gly contained 1089:1097 arg1 myelin Opalin AND N- myelin Opalin N- PUBTATOR Opalin 226115 Enzymatic deglycosylation showed that myelin Opalin contained N- and O-glycans, and that the O-glycans, at least, had negatively charged sialic acids. 3458201 0 77 gly 1B-glycoprotein 42:56 arg1 1B-glycoprotein 1B-glycoprotein PUBTATOR alpha 1B-glycoprotein 1 Amino acid sequence of human plasma alpha 1B-glycoprotein: homology to the immunoglobulin supergene family. 7947762 2 2 part_of RNase 397:401 arg1 position 7 RNase position 7 OGER SpecificSite RNase P07998 tryptophan residue at position 7 An aldohexopyranosyl residue was found to be linked via a C-C bond to the indole ring of the tryptophan residue at position 7 of human RNase Us. 9705299 6 45 gly glycosylated 1001:1012 arg1 G6Pase G6Pase PUBTATOR G6Pase 2538 Western blot and in vitro translation studies showed that G6Pase is glycosylated only at Asn96, further validating the nine-transmembrane topology model. 19276170 1 25 gly glycoprotein 240:251 arg1 thrombospondin-5 thrombospondin-5 PUBTATOR thrombospondin-5 1311 Cartilage oligomeric matrix protein (COMP), or thrombospondin-5 (TSP-5), is a secreted glycoprotein that is important for growth plate organization and function. 19276170 1 25 gly glycoprotein 240:251 arg1 Cartilage oligomeric matrix protein Cartilage oligomeric matrix protein OGER Cartilage oligomeric matrix protein P49747 Cartilage oligomeric matrix protein (COMP), or thrombospondin-5 (TSP-5), is a secreted glycoprotein that is important for growth plate organization and function. 11904304 1 25 gly glycosylated 263:274 arg1 c-Myc c-Myc PUBTATOR c-Myc 4609 Previously, we reported that c-Myc is glycosylated by O-linked N-acetylglucosamine at Thr-58, a known phosphorylation site and a mutational hot spot in lymphomas. 9054441 8 23 gly plasminogen 1365:1375 arg1 sialylation plasminogen sialylation OGER plasminogen P00747 These findings provide a structural basis for some of the observed microheterogeneity, and have implications with regard to the known functional consequences of the extent of sialylation of plasminogen. 9054441 8 55 gly sialylation 1350:1360 arg1 plasminogen plasminogen OGER plasminogen P00747 These findings provide a structural basis for some of the observed microheterogeneity, and have implications with regard to the known functional consequences of the extent of sialylation of plasminogen. 8349699 6 16 gly glycosylation 850:862 arg1 ovine PGH synthase-1 ovine PGH synthase-1 PUBTATOR PGH synthase-1 19224 N-Glycosylation consensus sequences corresponding to the three glycosylation sites of ovine PGH synthase-1 are conserved in the deduced amino acid sequences of PGH synthases-2. 20511397 0 48 gly Glycosylation 0:12 arg1 macrophage-derived human apolipoprotein E macrophage-derived human apolipoprotein E PUBTATOR apolipoprotein E 348 Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290. 20511397 0 55 gly E 73:73 arg1 sialylation apolipoprotein E sialylation PUBTATOR apolipoprotein E 348 Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290. 20511397 0 59 gly sialylation 18:28 arg1 macrophage-derived human apolipoprotein E macrophage-derived human apolipoprotein E PUBTATOR apolipoprotein E 348 Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290. 21752865 11 20 gly N-glycosylated 1756:1769 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 Our results confirm the theoretical predictions that BRI2 is N-glycosylated at Asn170 and show that this post-translational modification is essential for its expression at the cell surface but not for its proteolytic processing. 18715980 3 50 gly proteins 542:549 arg1 various N-linked carbohydrates SPINKL proteins various N-linked carbohydrates PUBTATOR SPINKL proteins 77424 Further analysis identified several SPINKL proteins with various N-linked carbohydrates. 21752865 7 28 gly N-glycosylated 1245:1258 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 These data indicate that BRI2 is N-glycosylated at Asn170. 11805077 2 43 gly found 386:390 arg1 EPO AND sulfated N-linked oligosaccharides EPO sulfated N-linked oligosaccharides OGER EPO P01588 Using LC/MS with GCC, sulfated N-linked oligosaccharides were found in erythropoietin (EPO) expressed in baby hamster kidney cells. 11805077 2 43 gly found 386:390 arg1 erythropoietin AND sulfated N-linked oligosaccharides erythropoietin sulfated N-linked oligosaccharides OGER erythropoietin P01588 Using LC/MS with GCC, sulfated N-linked oligosaccharides were found in erythropoietin (EPO) expressed in baby hamster kidney cells. 29402915 7 58 gly glycosylation 1340:1352 arg1 cubilin protein cubilin protein PUBTATOR cubilin protein 8029 Quantitative mass spectrometry and mutagenesis demonstrated that N-linked glycosylation of at least 4 residues of cubilin protein was required for its surface targeting. 8243461 6 19 gly deglycosylation 1288:1302 arg1 bovine factor X1 bovine factor X1 Cterm factor X1 The effect of deglycosylation of bovine factor X1 was investigated with factor-X-activating enzyme from Russell's viper venom or extrinsic Xase (factor VIIa/tissue factor/phospholipid) by examining the activation rates of derivatives of factor X prepared using O-glycanase, sialidase, and/or N-glycanase. 20378933 7 84 gly deglycosylation 1007:1021 arg1 IgA binding IgA binding OGER IgA P11912 Flow cytometry analysis of IgA binding to CHO cells transfected with mutated FcalphaR showed that deglycosylation of FcalphaR at individual N44, N120, N156, N165 or N177 site did not affect IgA binding but deglycosylation at N58 resulted in marked increase of IgA binding. 22809326 8 89 gly glycosylated 1032:1043 arg1 a heavily glycosylated lysosomal membrane protein a heavily glycosylated lysosomal membrane protein OGER glycosylated lysosomal membrane protein Q8WWB7 It is the first high-resolution structure of a heavily glycosylated lysosomal membrane protein. 8202534 7 26 gly homogeneity 986:996 arg1 SAP SAP PUBTATOR SAP 325 The protein and glycan homogeneity of SAP and the integrity of AP suggest that the complete glycoprotein structure is important for the normal and the pathophysiological functions of this molecule. 8202534 7 47 gly SAP 1001:1003 arg1 The protein and glycan homogeneity SAP The protein and glycan homogeneity PUBTATOR SAP 325 The protein and glycan homogeneity of SAP and the integrity of AP suggest that the complete glycoprotein structure is important for the normal and the pathophysiological functions of this molecule. 15003450 3 31 gly glycoprotein 465:476 arg1 the human alpha-GAL glycoprotein the human alpha-GAL glycoprotein OGER GAL glycoprotein Q8N6F7 Here, we present the structure of the human alpha-GAL glycoprotein determined by X-ray crystallography. 10889209 0 50 part_of Asn117 46:51 arg1 ROMK1 ROMK1 Asn117 PUBTATOR AminoAcid ROMK1 3758 Asn117 Glycosylation of GIRK1 at Asn119 and ROMK1 at Asn117 has different consequences in potassium channel function. 8130392 3 53 gly rHPC 570:573 arg1 the carbohydrate moiety rHPC the carbohydrate moiety OGER rHPC P52873 In seeking to elucidate the molecular basis for the improved efficacy of the recombinant antithrombotic drug, we focused on the carbohydrate moiety of rHPC. 7780197 6 35 part_of found 886:890 arg2 the TfR AND Asn-727 the TfR Asn-727 PUBTATOR SpecificSite TfR 7037 Asn-727 Glycosylation of Asn-727 found in the TfR purified from human placentae was analysed by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) and mass spectrometry following tryptic digestion, peptide purification via reverse-phase high-performance liquid chromatography (RP-HPLC) and peptide sequencing. 1997323 10 55 gly type 1120:1123 arg1 rTf-3 rTf type Cterm rTf 24825 Thus, in rTf-3, both antennae were of the disialylated diantennary N-acetyllactosamine type which is frequently encountered in other plasma glycoproteins. 23027977 1 23 gly glycoprotein 139:150 arg1 Enpp1 Enpp1 PUBTATOR Enpp1 5167 Enpp1 is a membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing extracellular nucleotide triphosphates to produce pyrophosphate. 18214858 4 60 gly haptoglobin 886:896 arg1 tri- haptoglobin tri- PUBTATOR haptoglobin 3240 Mass spectrometry analyses demonstrated that concentrations of total fucosylated di-, tri- and tetra-branched glycans of haptoglobin increased in the sera of PC patients. 18214858 4 60 gly haptoglobin 886:896 arg1 tetra-branched glycans haptoglobin tetra-branched glycans PUBTATOR haptoglobin 3240 Mass spectrometry analyses demonstrated that concentrations of total fucosylated di-, tri- and tetra-branched glycans of haptoglobin increased in the sera of PC patients. 18214858 4 60 gly haptoglobin 886:896 arg1 total fucosylated di- haptoglobin total fucosylated di- PUBTATOR haptoglobin 3240 Mass spectrometry analyses demonstrated that concentrations of total fucosylated di-, tri- and tetra-branched glycans of haptoglobin increased in the sera of PC patients. 22846177 5 54 part_of vimentin 736:743 arg1 vimentin serine 71 vimentin vimentin serine 71 PUBTATOR SpecificSite vimentin 7431 serine 71 The interaction with GlcNAc-bearing polymers promoted the cell surface recruitment of vimentin followed by the phosphorylation of vimentin serine 71 and the increase in tetrameric vimentin disassembled from vimentin filaments in HeLa cells. 2498325 4 56 gly glycosylated 570:581 arg1 asialo-apoE asialo-apoE PUBTATOR apoE 348 Sequence analysis and amino sugar analysis of this peptide derived from asialo-, monosialo-, or disialo-apoE indicated that the carbohydrate moiety is attached only to Thr194 in monosialo- and disialo-apoE and that asialo-apoE is not glycosylated. 24967714 0 4 gly gp120 61:65 arg1 the highly conserved N-glycan at Asn260 gp120 the highly conserved N-glycan at Asn260 PUBTATOR gp120 155971 Deletion of the highly conserved N-glycan at Asn260 of HIV-1 gp120 affects folding and lysosomal degradation of gp120, and results in loss of viral infectivity. 2415652 4 36 gly beta 1033:1036 arg1 the O-linked oligosaccharides hCG beta the O-linked oligosaccharides PUBTATOR hCG beta 1082 Oligosaccharides, O-linked to serine or threonine, were present in ElBre hCG beta, presumably on its CTE as judged by the complete binding of desialylated ElBre hCG beta to immobilized peanut agglutinin (this lectin is specific for terminal galactose linked beta 1----3 to N-acetylgalactosamine, a disaccharide exposed after desialylation of the O-linked oligosaccharides of standard hCG beta). 2415652 4 110 gly desialylated 787:798 arg1 desialylated ElBre hCG beta desialylated ElBre hCG beta PUBTATOR ElBre hCG beta 1082 Oligosaccharides, O-linked to serine or threonine, were present in ElBre hCG beta, presumably on its CTE as judged by the complete binding of desialylated ElBre hCG beta to immobilized peanut agglutinin (this lectin is specific for terminal galactose linked beta 1----3 to N-acetylgalactosamine, a disaccharide exposed after desialylation of the O-linked oligosaccharides of standard hCG beta). 2415652 4 98 gly present 701:707 arg2 ElBre hCG beta AND Oligosaccharides ElBre hCG beta Oligosaccharides PUBTATOR ElBre hCG beta 1082 Oligosaccharides, O-linked to serine or threonine, were present in ElBre hCG beta, presumably on its CTE as judged by the complete binding of desialylated ElBre hCG beta to immobilized peanut agglutinin (this lectin is specific for terminal galactose linked beta 1----3 to N-acetylgalactosamine, a disaccharide exposed after desialylation of the O-linked oligosaccharides of standard hCG beta). 22688517 2 57 gly N-glycosylation 216:230 arg1 LOX-1 LOX-1 PUBTATOR LOX-1 4973 The N-glycosylation of LOX-1 has been shown to affect its biological functions in vivo and modulate the pathogenesis of atherosclerosis. 3571235 2 2 gly sialoglycoproteins 157:174 arg1 glycophorins B and C glycophorins B and C PUBTATOR glycophorins B and C 2994 We have developed methods for the preparative purification of two sialoglycoproteins (glycophorins B and C) from human erythrocyte membranes by high-performance ion exchange and gel permeation chromatography in the presence of Triton X-100. 16083266 8 86 gly glycosylated 2417:2428 arg1 beta-casein beta-casein OGER beta-casein P05814 Using ERPA, we demonstrate >95% sequence coverage in the analysis of two heavily phosphorylated and glycosylated proteins, beta-casein at the 50 fmole level and the epidermal growth factor receptor (EGFR) at the 1 pmole level. 16083266 8 86 gly glycosylated 2417:2428 arg1 the epidermal growth factor receptor the epidermal growth factor receptor PUBTATOR epidermal growth factor receptor 1956 Using ERPA, we demonstrate >95% sequence coverage in the analysis of two heavily phosphorylated and glycosylated proteins, beta-casein at the 50 fmole level and the epidermal growth factor receptor (EGFR) at the 1 pmole level. 8407880 0 57 gly factor 71:76 arg1 the N-linked oligosaccharides hepatocyte growth factor the N-linked oligosaccharides PUBTATOR hepatocyte growth factor 24446 Structural study of the N-linked oligosaccharides of hepatocyte growth factor by two-dimensional sugar mapping. 2793860 6 60 gly site 1048:1051 arg1 natural human IL-2 IL-2 site PUBTATOR IL-2 P60568 The O-linked chains were exclusively linked to Thr in position 3 of the polypeptide chain which is the carbohydrate attachment site in natural human IL-2. 1731338 0 92 gly galactoglycoprotein 36:54 arg1 human plasma galactoglycoprotein human plasma galactoglycoprotein PUBTATOR galactoglycoprotein 6693 Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin). 19592704 5 41 gly glycosylation 793:805 arg1 NCEH NCEH PUBTATOR NCEH 57552 All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 19592704 5 53 gly glycosylated 860:871 arg1 NCEH NCEH PUBTATOR NCEH 57552 All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 27313224 3 14 gly glycoprotein 586:597 arg1 vesicle glycoprotein 2 vesicle glycoprotein 2 PUBTATOR synaptic vesicle glycoprotein 2 9900 The luminal domain 4 (LD4) of the three synaptic vesicle glycoprotein 2 (SV2) isoforms A-C mediates uptake of the clinically most relevant serotype BoNT/A1. 27313224 3 14 gly glycoprotein 586:597 arg1 SV2 SV2 PUBTATOR SV2 9900 The luminal domain 4 (LD4) of the three synaptic vesicle glycoprotein 2 (SV2) isoforms A-C mediates uptake of the clinically most relevant serotype BoNT/A1. 21768335 3 54 gly afucosylated 471:482 arg1 afucosylated IgGs afucosylated IgGs Cterm IgGs Although afucosylated IgGs exist naturally, a next generation of recombinant therapeutic, glycoenginereed antibodies is currently being developed to exploit this finding. 14573609 2 6 gly glycosylated 398:409 arg1 glycosylated heparanase glycosylated heparanase PUBTATOR heparanase 10855 The predicted amino acid sequence of heparanase includes six putative N-glycosylation sites; however, the precise biochemical role of glycosylated heparanase remains unknown. 24977290 4 76 gly presence 627:634 arg1 plasma derived ADAMTS13 AND putative O-fucosylation, C-mannosylation and N-linked glycosylation sites plasma derived ADAMTS13 putative O-fucosylation, C-mannosylation and N-linked glycosylation sites PUBTATOR ADAMTS13 Q76LX8 OBJECTIVES: Here we investigated the presence of putative O-fucosylation, C-mannosylation and N-linked glycosylation sites on plasma derived ADAMTS13. 8460945 1 61 gly attached 198:205 arg1 human erythropoietin AND the mucin-type sugar chains human erythropoietin the mucin-type sugar chains PUBTATOR erythropoietin 2056 Less is known about the mucin-type sugar chains attached to human erythropoietin as compared with N-linked sugar chains which structures and function have been well studied. 26563299 12 17 gly glycosylation 1741:1753 arg1 IL-15 IL-15 PUBTATOR IL-15 3600 Highly reproducible glycosylation of IL-15 and sIL-15Rα of two batches of hetIL-15 demonstrated consistent manufacturing and purification. 19379732 4 23 gly N-glycosylation 1378:1392 arg1 hSVCT1 membrane targeting hSVCT1 membrane targeting PUBTATOR hSVCT1 9963 However, the N138Q/N144Q double mutant displayed barely detectable membrane expression at approximately 65 kDa, no apparent glycosylation and minimal AA uptake (<10%) with no discernible improvement in expression or activity when cultured at 28 degrees C or 37 degrees C. Marker protein immunocytochemistry with N138Q/N144Q identified intracellular aggregates with hSVCT1 localised at the nuclear membrane but absent at the plasma membrane thus implicating its role as a possible intracellular transporter and suggesting N-glycosylation is required for hSVCT1 membrane targeting. 8639592 8 87 gly structures 1501:1510 arg1 r-hTFPI hTFPI structures OGER hTFPI P10646 All the N-linked structures in r-hTFPI were complex-type carbohydrate chains with one fucose residue attached to the reducing-end GlcNAc and consisted of bi-, tri-, and tetraantennary carbohydrate chains in the ratio 1.9:1.3:1.0. 9153399 8 45 gly ICAM-2 1268:1273 arg1 N-linked glycans ICAM-2 N-linked glycans PUBTATOR ICAM-2 3384 A bend between domains 1 and 2 of ICAM-2 and a tripod-like arrangement of N-linked glycans in the membrane-proximal region of domain 2 may be important for presenting the recognition surface to LFA-1. 16834341 3 33 gly glycopeptides 370:382 arg1 human pituitary follicle stimulating hormone human pituitary follicle stimulating hormone Cterm follicle stimulating hormone In the present study the negatively charged glycopeptides of equine and human pituitary follicle stimulating hormone (eFSH and hFSH) have been characterized in a glycosylation site-specific manner using FT-ICR-MS and Edman sequencing. 7679920 0 45 gly alpha-fetoprotein 33:49 arg1 Carbohydrate structures alpha-fetoprotein Carbohydrate structures PUBTATOR alpha-fetoprotein 174 Carbohydrate structures of human alpha-fetoprotein of patients with hepatocellular carcinoma: presence of fucosylated and non-fucosylated triantennary glycans. 10929010 0 43 gly transferrin 27:37 arg1 N-glycan patterns transferrin N-glycan patterns PUBTATOR transferrin 7018 N-glycan patterns of human transferrin produced in Trichoplusia ni insect cells: effects of mammalian galactosyltransferase. 20511397 3 77 gly structures 590:599 arg1 plasma apoE apoE structures PUBTATOR apoE 348 Some of the glycan structures on plasma apoE are characterized; however, the more complicated structures on plasma and cellular/secreted apoE remain unidentified. 2498325 9 15 gly N-glycosylated 1435:1448 arg1 this apoE this apoE PUBTATOR apoE 348 Studies with tunicamycin indicated that this apoE was N-glycosylated at Asn194. 8486673 4 46 gly CRP 452:454 arg1 the oligosaccharide chain CRP the oligosaccharide chain PUBTATOR CRP 25419 In this study, the nature of the oligosaccharide chain of rat CRP was investigated by fast atom bombardment-mass spectrometry (FAB-MS), and general features of its biosynthetic pathway were also analyzed. 10669592 4 1 part_of NEP 721:723 arg1 residues 52-749 NEP residues 52-749 PUBTATOR SpecificSite NEP 4311 residues 52-749 Here we describe the crystal structure of the extracellular domain (residues 52-749) of human NEP complexed with the generic metalloproteinase inhibitor phosphoramidon at 2.1 A resolution. 12435857 9 15 gly glycosylation 1876:1888 arg1 misfolded THTR-1 protein misfolded THTR-1 protein PUBTATOR THTR-1 protein 10560 CONCLUSIONS: Taken collectively, these results suggest that the G172D mutation presumably misfolded THTR-1 protein that fails to undergo a complete glycosylation, is retained in the Golgi-ER compartment and thereby cannot be targeted to the plasma membrane. 7686446 5 41 gly isoform 1527:1533 arg1 the sugar chain structure AFP isoform the sugar chain structure PUBTATOR AFP isoform 174 In contrast, the sugar chain structure of HCC highly specific AFP isoform was found to comprise a monosialyl-biantennary sugar chain with additional fucosylation of the proximal N-acetylglucosamine. 8390218 1 69 gly 1-antitrypsin 191:203 arg1 the sugar chains alpha 1-antitrypsin the sugar chains PUBTATOR alpha 1-antitrypsin 5265 Chemical structures of the sugar chains of alpha 1-antitrypsin (AAT) from patients with hepatocellular carcinoma (HCC) and from healthy individuals with a different affinity for Lens culinaris agglutinin (LCA) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion in combination with reversed-phase and size-fractionation high-performance liquid chromatography. 16332679 7 5 gly O-glycosylation 937:951 arg1 Sp1 Sp1 OGER Sp1 P08047 The O-glycosylation of Sp1 and its nuclear accumulation induced by insulin peaked early (approximately 30 min), followed by a steady decline of O-GlcNAc-modified Sp1 to negligible levels by 240 min. 21606496 4 7 gly glycosylation 543:555 arg1 2B4 2B4 PUBTATOR 2B4 51744 Using a recombinant fusion protein of the extracellular domain of 2B4, we demonstrate that N-linked glycosylation of 2B4 is essential for the binding to its ligand CD48. 18061361 0 28 gly glycosylation 63:75 arg1 the sodium-coupled bicarbonate transporter NCBE the sodium-coupled bicarbonate transporter NCBE OGER bicarbonate transporter NCBE Q9Y6M7 Use of a new polyclonal antibody to study the distribution and glycosylation of the sodium-coupled bicarbonate transporter NCBE in rodent brain. 7947762 0 16 gly C-glycosylation 58:72 arg1 human RNase Us human RNase Us OGER RNase P07998 New type of linkage between a carbohydrate and a protein: C-glycosylation of a specific tryptophan residue in human RNase Us. 9757569 0 23 gly glycosylation 11:23 arg1 the human renin reaction the human renin reaction PUBTATOR renin 5972 Effects of glycosylation of the residue at position 14 in ovine angiotensinogen on the human renin reaction. 25617829 0 15 gly N-glycosylation 0:14 arg1 GPRC6A receptor expression GPRC6A receptor PUBTATOR GPRC6A receptor 222545 N-glycosylation and disulfide bonding affects GPRC6A receptor expression, function, and dimerization. 1737041 5 53 gly GM-CSF 1122:1127 arg1 several carbohydrate-deficient mutants GM-CSF several carbohydrate-deficient mutants PUBTATOR GM-CSF 1437 Unlike a number of other secreted glycoproteins, the transient time and secretory efficiency of several carbohydrate-deficient mutants of GM-CSF are indistinguishable from those of the native growth factor in BHK, 293, COS, and ldlD cells. 18490449 11 99 gly sialylglycosylated 1747:1764 arg1 highly sialylglycosylated Opalin highly sialylglycosylated Opalin PUBTATOR Opalin 226115 These results suggest a role for highly sialylglycosylated Opalin in an intermembranous function of the myelin paranodal loops in the central nervous system. 2108149 9 1 gly glycosylation 1654:1666 arg1 IgGs IgGs Cterm IgGs Analysis of the carbohydrate moieties of the gamma 1 chain from the homodimeric and heterodimeric IgGs and of the gamma 2b chain from the heterodimeric molecule demonstrates that the polypeptide structure of the heavy chain influences the terminal galactosylation of the glycan unit at the conserved site of glycosylation of IgGs. 2108149 9 80 gly IgGs 1444:1447 arg1 the carbohydrate moieties IgGs the carbohydrate moieties Cterm IgGs Analysis of the carbohydrate moieties of the gamma 1 chain from the homodimeric and heterodimeric IgGs and of the gamma 2b chain from the heterodimeric molecule demonstrates that the polypeptide structure of the heavy chain influences the terminal galactosylation of the glycan unit at the conserved site of glycosylation of IgGs. 22750213 4 34 gly glycoprotein 682:693 arg1 PRiMA PRiMA PUBTATOR PRiMA 170952 PRiMA is a glycoprotein containing two putative N-linked glycosylation sites. 22766194 9 5 gly N-glycosylation 1567:1581 arg1 NEP activities NEP activities PUBTATOR NEP 4311 CONCLUSIONS: N-glycosylation at Asn(628) is essential not only for NEP activities, but also for surface expression. 24967714 2 62 gly present 341:347 arg2 gp120 AND 24 N-linked glycans gp120 24 N-linked glycans PUBTATOR gp120 3700 Of the, on average, 24 N-linked glycans present on gp120, the glycan at Asn260 was reported to be essential for the correct expression of gp120 and gp41 in the virus particle and deletion of the N260 glycan in gp120 heavily compromised virus infectivity. 2243102 6 49 gly located 942:948 arg1 lamp-2 AND polylactosaminoglycans lamp-2 polylactosaminoglycans PUBTATOR lamp-2 3920 Amino acid analysis and sequencing demonstrated that polylactosaminoglycans were located at Asn-34, Asn-93 and/or Asn-102, and Asn-195 and/or Asn-200 in lamp-1, and at Asn-4 and/or Asn-10, and Asn-279 in lamp-2. 3200844 3 43 gly glycoprotein 402:413 arg1 np62 np62 PUBTATOR np62 65274 The major (62 kDa) nuclear pore glycoprotein (np62) was purified from rat liver nuclear envelopes by immunoaffinity chromatography and preparative gel electrophoresis. 9336835 1 78 gly glycoprotein 126:137 arg1 Clusterin Clusterin PUBTATOR Clusterin 1191 Clusterin is a ubiquitous, heterodimeric glycoprotein with multiple possible functions that are likely influenced by glycosylation. 10978165 6 14 gly glycosylated 659:670 arg1 1-74 1-74 Cterm 1-74 6358 These peptides were isolated from a peptide library of human blood filtrate and represent predominantly HCC-1 (1-74) and glycosylated HCC-1 (1-74). 10978165 6 14 gly glycosylated 659:670 arg1 glycosylated HCC-1 glycosylated HCC-1 PUBTATOR HCC-1 6358 These peptides were isolated from a peptide library of human blood filtrate and represent predominantly HCC-1 (1-74) and glycosylated HCC-1 (1-74). 17591618 0 70 gly factor 60:65 arg1 Site-specific N-glycan characterization complement factor H Site-specific N-glycan characterization PUBTATOR complement factor H 3075 Site-specific N-glycan characterization of human complement factor H. Human complement factor H (CFH) is a plasma glycoprotein involved in the regulation of the alternative pathway of the complement system. 21056543 1 2 gly glycoprotein 129:140 arg1 Protein C inhibitor Protein C inhibitor PUBTATOR Protein C inhibitor 5104 Protein C inhibitor (PCI) is a 57-kDa glycoprotein that exists in many tissues and secretions in human. 29402915 6 52 gly glycosylation 1197:1209 arg1 cubilin cubilin PUBTATOR cubilin 8029 Notably, the interaction between cubilin and amnionless was not sufficient, but amnionless-mediated glycosylation of cubilin was necessary for their surface expression. 2943741 6 77 gly glycosylated 1415:1426 arg1 leukosialin leukosialin PUBTATOR leukosialin P16150 These results clearly indicate that leukosialin can be differently glycosylated with O-linked chains, and each erythroid or myeloid (and T-lymphoid) cell line expresses a characteristic set of O-linked oligosaccharides which differ in core structures as well as in sialylation. 2129367 10 3 gly presence 1299:1306 arg2 protein Z AND the unique trisaccharide structure protein Z the unique trisaccharide structure Cterm protein Z The presence of the unique trisaccharide structure in factors VII, IX and protein Z leads us to anticipate its biological role in the tissue factor pathway. 2129367 10 3 gly presence 1299:1306 arg1 factors VII AND the unique trisaccharide structure factors VII the unique trisaccharide structure Cterm VII The presence of the unique trisaccharide structure in factors VII, IX and protein Z leads us to anticipate its biological role in the tissue factor pathway. 18340083 6 22 gly glycan 846:851 arg1 GPIHBP1 GPIHBP1 glycan PUBTATOR GPIHBP1 68453 The glycan was marginally sensitive to endoglycosidase F2 digestion but resistant to endoglycosidase F3 digestion, suggesting that the glycan on GPIHBP1 is of the oligomannose type. 8223648 5 62 gly carry 502:506 arg1 natural IFN-omega 1 AND complex-type oligosaccharides natural IFN-omega 1 complex-type oligosaccharides PUBTATOR IFN-omega 1 3467 While natural IFN-omega 1 was shown to carry complex-type oligosaccharides [Adolf, G. R., Maurer-Fogy, I., Kalsner, I. & Cantell, K. (1990) J. Biol. 16537470 3 86 gly chain 621:625 arg1 the galactose moiety chain the galactose moiety OGER chain 12479 For GalA-GSL, a shorter fatty acyl chain, an absence of the 4-OH on the sphingosine tail and a 6'-COOH group on the galactose moiety account for its observed antigenic potency. 20457942 2 13 gly glycoprotein 172:183 arg1 RhCG RhCG PUBTATOR RhCG 51458 Human Rh C glycoprotein (RhCG) forms a trimeric complex that plays an essential role in ammonia excretion and renal pH regulation. 20457942 2 13 gly glycoprotein 172:183 arg1 Human Rh C glycoprotein Human Rh C glycoprotein PUBTATOR Human Rh C glycoprotein 51458 Human Rh C glycoprotein (RhCG) forms a trimeric complex that plays an essential role in ammonia excretion and renal pH regulation. 15066430 1 6 gly glycoprotein 208:219 arg1 Carboxypeptidase M Carboxypeptidase M PUBTATOR Carboxypeptidase M 1368 Carboxypeptidase M (CPM), an extracellular glycosylphosphatidyl-inositol(GPI)-anchored membrane glycoprotein belonging to the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, specifically removes C-terminal basic residues from peptides and proteins. 24884609 10 23 gly O-glycoforms 1413:1424 arg1 isoform-specific ITIH4 O-glycoforms isoform-specific ITIH4 O-glycoforms PUBTATOR ITIH4 3700 We also identified isoform-specific ITIH4 O-glycoforms and documented that utilization of O-glycosylation sites on ITIH4 differed between the cell line and serum. 14711516 6 3 gly Recombinant 1003:1013 arg1 GlcNAc Recombinant Thy-1 GlcNAc PUBTATOR Recombinant Thy-1 P04216 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 3 gly Recombinant 1003:1013 arg1 2 Recombinant Thy-1 2 PUBTATOR Recombinant Thy-1 P04216 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 3 gly Recombinant 1003:1013 arg1 Man Recombinant Thy-1 Man PUBTATOR Recombinant Thy-1 P04216 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 3 gly Recombinant 1003:1013 arg1 5 Recombinant Thy-1 5 PUBTATOR Recombinant Thy-1 P04216 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 41 gly oligosaccharides 1097:1112 arg1 asparagine 23 and 100 asparagine 23 and 100 asparagine 23 and 100 SpecificSite asparagine 23 and 100 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 41 gly oligosaccharides 1097:1112 arg1 100 100 100 SpecificSite asparagine 23 and 100 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 3571235 4 1 gly sialoglycoprotein 624:640 arg1 glycophorin A glycophorin A PUBTATOR glycophorin A 2993 The amino acid sequence of the intramembranous domain (residues 36-71) of glycophorin B was determined and found to be similar to that of the hydrophobic region of the major sialoglycoprotein (glycophorin A). 22226965 3 36 gly modified 487:494 arg1 PPARγ AND O-GlcNAc PPARγ O-GlcNAc PUBTATOR PPARγ 19016 Here, we report that PPARγ is modified by O-GlcNAc in 3T3-L1 adipocytes. 26271046 10 56 part_of GluA2 1803:1807 arg1 the N413 GluA2 the N413 PUBTATOR SpecificSite GluA2 2891 N413 The HNK-1 epitope on N-glycan at the N413 of GluA2 was also involved in the cell surface expression of GluA1. 21980294 0 63 gly glycoprotein 34:45 arg1 herpes simplex virus glycoprotein D herpes simplex virus glycoprotein D PUBTATOR glycoprotein D 2532 Structure of herpes simplex virus glycoprotein D bound to the human receptor nectin-1. 2215480 1 35 gly glycoprotein 227:238 arg1 T-cell-replacing factor (TRF)/IL-5 T-cell-replacing factor (TRF)/IL-5 PUBTATOR IL-5 16191 T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. 25485983 1 41 gly glycosylation 114:126 arg1 antithrombin antithrombin PUBTATOR antithrombin 462 The inefficient glycosylation of consensus sequence on N135 in antithrombin explains the two glycoforms of this key anticoagulant serpin found in plasma: α and β, with four and three N-glycans, respectively. 15454184 1 51 gly glycoprotein 135:146 arg1 Follistatin Follistatin PUBTATOR Follistatin (FS) 10468 Follistatin (FS), a glycoprotein, plays an important role in cell growth and differentiation through the neutralization of the biological activities of activins. 16877748 0 27 gly molecule-1 86:95 arg1 N-glycan structures intercellular adhesion molecule-1 N-glycan structures PUBTATOR intercellular adhesion molecule-1 15894 N-glycan structures and N-glycosylation sites of mouse soluble intercellular adhesion molecule-1 revealed by MALDI-TOF and FTICR mass spectrometry. 16877748 0 53 gly N-glycosylation 24:38 arg1 mouse soluble intercellular adhesion molecule-1 mouse soluble intercellular adhesion molecule-1 PUBTATOR intercellular adhesion molecule-1 15894 N-glycan structures and N-glycosylation sites of mouse soluble intercellular adhesion molecule-1 revealed by MALDI-TOF and FTICR mass spectrometry. 15498570 0 24 gly glycoprotein 78:89 arg1 hPAP21 hPAP21 PUBTATOR hPAP21 84279 N-glycosylation is required for efficient secretion of a novel human secreted glycoprotein, hPAP21. 15498570 0 41 gly N-glycosylation 0:14 arg1 hPAP21 hPAP21 PUBTATOR hPAP21 84279 N-glycosylation is required for efficient secretion of a novel human secreted glycoprotein, hPAP21. 21757702 2 82 part_of Notch1 312:317 arg1 mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2) Notch1 mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2) PUBTATOR SpecificSite Notch1 18128 sequence C(1) Here we examine the occupancy of the predicted O-glucose sites on mouse Notch1 (mN1) using the consensus sequence C(1)XSXPC(2). 10871631 0 82 gly glycoproteins 70:82 arg1 Torsin A Torsin A PUBTATOR Torsin A 1861 Torsin A and its torsion dystonia-associated mutant forms are lumenal glycoproteins that exhibit distinct subcellular localizations. 2511201 4 0 gly contain 816:822 arg1 protein Z AND such carbohydrate moieties protein Z such carbohydrate moieties Cterm protein Z We report here that human factors VII and IX and protein Z and bovine protein Z also contain such carbohydrate moieties linked to a serine residue at the same position found in bovine factors VII and IX. 2511201 4 0 gly contain 816:822 arg1 bovine protein Z AND such carbohydrate moieties bovine protein Z such carbohydrate moieties Cterm bovine protein Z We report here that human factors VII and IX and protein Z and bovine protein Z also contain such carbohydrate moieties linked to a serine residue at the same position found in bovine factors VII and IX. 2511201 4 0 gly contain 816:822 arg1 human factors VII AND such carbohydrate moieties human factors VII such carbohydrate moieties Cterm VII We report here that human factors VII and IX and protein Z and bovine protein Z also contain such carbohydrate moieties linked to a serine residue at the same position found in bovine factors VII and IX. 17395588 0 101 gly O-fucosylation 0:13 arg1 ADAMTS-like-1/punctin-1 ADAMTS-like-1 O-fucosylation PUBTATOR ADAMTS-like-1 92949 O-fucosylation of thrombospondin type 1 repeats in ADAMTS-like-1/punctin-1 regulates secretion: implications for the ADAMTS superfamily. 21606496 2 56 gly glycosylated 276:287 arg1 2B4 2B4 PUBTATOR 2B4 51744 Here we show that 2B4 is heavily and differentially glycosylated in primary human NK cells and NK cell lines. 12200435 4 46 gly glycoprotein 645:656 arg1 Human Dpl Human Dpl PUBTATOR Human Dpl 23627 Human Dpl appears to be a glycosylphosphatidylinositol-anchored glycoprotein with N- and O-linked sugars. 21500857 6 4 gly contains 735:742 arg1 AP180 AND a phosphorylated O-GlcNAc AP180 a phosphorylated O-GlcNAc PUBTATOR AP180 65178 We now show that AP180 purified from rat brain contains a phosphorylated O-GlcNAc (O-GlcNAc-P) within a highly conserved sequence. 21500857 6 4 gly contains 735:742 arg1 AP180 AND O-GlcNAc-P AP180 O-GlcNAc-P PUBTATOR AP180 65178 We now show that AP180 purified from rat brain contains a phosphorylated O-GlcNAc (O-GlcNAc-P) within a highly conserved sequence. 8999954 4 7 gly detected 483:490 arg2 mouse ER AND O-GlcNAc moieties mouse ER O-GlcNAc moieties PUBTATOR ER 17289 O-GlcNAc moieties were detected on insect cell-expressed, mouse ER (mER) by probing with bovine milk galactosyltransferase, followed by structural analysis. 19951703 7 57 gly unglycosylated 943:956 arg1 The unglycosylated UGT1A9 The unglycosylated UGT1A9 PUBTATOR UGT1A9 54600 The unglycosylated UGT1A9 was almost inactive, which was not an indirect effect of ER stress. 9884403 1 0 gly N-glycosylation 93:107 arg1 human Tamm-Horsfall glycoprotein human Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 The N-glycosylation sites of human Tamm-Horsfall glycoprotein from one healthy male donor have been characterized, based on an approach using endoproteinase Glu-C (V-8 protease, Staphylococcus aureus ) digestion and a combination of chromatographic techniques, automated Edman sequencing, and fast atom bombardment mass spectrometry. 9884403 1 45 gly glycoprotein 138:149 arg1 human Tamm-Horsfall glycoprotein human Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 The N-glycosylation sites of human Tamm-Horsfall glycoprotein from one healthy male donor have been characterized, based on an approach using endoproteinase Glu-C (V-8 protease, Staphylococcus aureus ) digestion and a combination of chromatographic techniques, automated Edman sequencing, and fast atom bombardment mass spectrometry. 1457416 0 28 gly 2HS-glycoprotein 71:86 arg1 2HS-glycoprotein 2HS-glycoprotein PUBTATOR alpha 2HS-glycoprotein 197 Structure of the N- and O-glycans of the A-chain of human plasma alpha 2HS-glycoprotein as deduced from the chemical compositions of the derivatives prepared by stepwise degradation with exoglycosidases. 18768590 7 64 gly glycosylated 1189:1200 arg1 The complexly glycosylated TRPV5 The complexly glycosylated TRPV5 OGER TRPV5 Q9NQA5 The complexly glycosylated TRPV5 that appears at the plasma membrane was increased by WNK3. 24365146 2 48 gly glycosylation 219:231 arg1 human Nox1 human Nox1 PUBTATOR Nox1 27035 Here we show that human Nox1 undergoes glycosylation at Asn-162 and Asn-236 in the second and third extracellular loops, respectively. 7107587 0 33 gly fibrinogen 32:41 arg1 Carbohydrate structure fibrinogen Carbohydrate structure PUBTATOR fibrinogen 2244 Carbohydrate structure of human fibrinogen. 10814696 2 64 gly glycosylation 563:575 arg1 arylsulfatase A arylsulfatase A PUBTATOR arylsulfatase A 410 To understand the significance of any changes in the glycosylation of arylsulfatase A in cancer, it is important to know the structure of its carbohydrate component in normal tissue. 24927598 7 26 gly glycosylation 953:965 arg1 ZIP14 ZIP14 PUBTATOR ZIP14 23516 Asparagine-linked (N-linked) glycosylation of ZIP14, particularly the glycosylation at N102, was required for efficient membrane extraction of ZIP14 and therefore is necessary for its iron sensitivity. 24884609 5 68 part_of ITIH4 665:669 arg1 N517 ITIH4 N81, N207, N517, and N577 PUBTATOR SpecificSite ITIH4 3700 N81, N207, N517, and N577 First, we confirmed that the four ITIH4 N-X-S/T sequons (N81, N207, N517, and N577) were glycosylated by treating ITIH4 tryptic/GluC glycopeptides with PNGaseF in the presence of (18)O water. 24884609 5 68 part_of ITIH4 665:669 arg1 N577 ITIH4 N81, N207, N517, and N577 PUBTATOR SpecificSite ITIH4 3700 N81, N207, N517, and N577 First, we confirmed that the four ITIH4 N-X-S/T sequons (N81, N207, N517, and N577) were glycosylated by treating ITIH4 tryptic/GluC glycopeptides with PNGaseF in the presence of (18)O water. 24884609 5 68 part_of ITIH4 665:669 arg1 N207 ITIH4 N81, N207, N517, and N577 PUBTATOR SpecificSite ITIH4 3700 N81, N207, N517, and N577 First, we confirmed that the four ITIH4 N-X-S/T sequons (N81, N207, N517, and N577) were glycosylated by treating ITIH4 tryptic/GluC glycopeptides with PNGaseF in the presence of (18)O water. 24884609 5 68 part_of ITIH4 665:669 arg1 N577 ITIH4 N81, N207, N517, and N577 PUBTATOR SpecificSite ITIH4 3700 N81, N207, N517, and N577 First, we confirmed that the four ITIH4 N-X-S/T sequons (N81, N207, N517, and N577) were glycosylated by treating ITIH4 tryptic/GluC glycopeptides with PNGaseF in the presence of (18)O water. 24884609 5 68 part_of ITIH4 665:669 arg1 N207 ITIH4 N81, N207, N517, and N577 PUBTATOR SpecificSite ITIH4 3700 N81, N207, N517, and N577 First, we confirmed that the four ITIH4 N-X-S/T sequons (N81, N207, N517, and N577) were glycosylated by treating ITIH4 tryptic/GluC glycopeptides with PNGaseF in the presence of (18)O water. 24884609 5 68 part_of ITIH4 665:669 arg1 N207 ITIH4 N81, N207, N517, and N577 PUBTATOR SpecificSite ITIH4 3700 N81, N207, N517, and N577 First, we confirmed that the four ITIH4 N-X-S/T sequons (N81, N207, N517, and N577) were glycosylated by treating ITIH4 tryptic/GluC glycopeptides with PNGaseF in the presence of (18)O water. 29932112 6 13 part_of Panx2 886:890 arg1 N86 Panx2 N86 PUBTATOR SpecificSite Panx2 56666 N86 Our results showed that N86 is the only N-glycosylation site of Panx2. 2498325 7 68 part_of Apolipoprotein 945:958 arg1 Thr194----Ala Apolipoprotein E Thr194----Ala PUBTATOR AminoAcid Apolipoprotein E 348 Thr194 Apolipoprotein E(Thr194----Ala) was secreted exclusively as the asialo isoform, confirming that Thr194 is the site of carbohydrate attachment in these cells and indicating that glycosylation of apoE is not essential for secretion. 24816101 4 9 gly glycosylated 639:650 arg1 glycosylated SGSH glycosylated SGSH OGER SGSH P51688 Here, the crystal structure of glycosylated SGSH is presented at 2 Å resolution. 21402931 8 37 gly Nonpolymerizing 1285:1299 arg1 betaglycan Nonpolymerizing ZP betaglycan Cterm Nonpolymerizing ZP Nonpolymerizing ZP proteins such as betaglycan and endoglin do not contain this cleavage site. 21402931 8 52 gly ZP 1301:1302 arg1 betaglycan Nonpolymerizing ZP betaglycan Cterm Nonpolymerizing ZP Nonpolymerizing ZP proteins such as betaglycan and endoglin do not contain this cleavage site. 20739279 9 15 gly glycosylation 1134:1146 arg1 SynCAM 2 SynCAM 2 PUBTATOR SynCAM 2 253559 Although glycosylation of SynCAM 2 at Asn(60) reduces adhesion, N-glycans at Asn(70)/Asn(104) of SynCAM 1 increase its interactions. 8486673 10 5 gly non-glycosylated 1587:1602 arg1 The non-glycosylated rat CRP The non-glycosylated rat CRP PUBTATOR CRP 25419 The non-glycosylated rat CRP, however, was still able to bind to phosphorylcholine-Sepharose and to be secreted by hepatocytes. 18642129 1 6 gly glycoprotein 141:152 arg1 Factor VII Factor VII OGER Factor VII P08709 Factor VII (FVII) is a vitamin K-dependent glycoprotein which, in its activated form (FVIIa), participates in the coagulation process by activating factor X and factor IX. 10861210 2 70 gly contained 502:510 arg1 Human AE1 AND a high-mannose oligosaccharide Human AE1 a high-mannose oligosaccharide PUBTATOR Human AE1 6521 Human AE1 expressed in transfected human embryonic kidney (HEK)-293 or COS-7 cells contained a high-mannose oligosaccharide. 2015821 0 70 gly 1-antichymotrypsin 60:77 arg1 the glycans alpha 1-antichymotrypsin the glycans PUBTATOR alpha 1-antichymotrypsin 12 Structure determination of the glycans of human-serum alpha 1-antichymotrypsin using 1H-NMR spectroscopy and deglycosylation by N-glycanase. 1710976 12 12 part_of Peptide 1287:1293 arg1 residues 427-429 Peptide residues 427-429 OGER SpecificSite Peptide residues 427-429 Peptide Asn-Gly-Ser (residues 427-429) is the most probable candidate for glycosylation; literature data suggests that deamidation occurs in the stretch Glu-Asn-Gly-Lys-Asp (residues 364-368) and Asn-Gly-Asn-Cys (residues 474-477). 19855092 0 33 gly adiponectin 28:38 arg1 Sialic acid modification adiponectin Sialic acid modification PUBTATOR adiponectin 11450 Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation. 19855092 0 96 gly modification 12:23 arg1 adiponectin AND Sialic acid modification adiponectin Sialic acid modification PUBTATOR adiponectin 11450 Sialic acid modification of adiponectin is not required for multimerization or secretion but determines half-life in circulation. 26563299 3 32 gly glycosylation 531:543 arg1 hetIL-15 hetIL-15 PUBTATOR IL-15 3600 Favorable pharmacokinetic properties are associated with the heterodimeric formation and the glycosylation of hetIL-15, which, however, remains largely uncharacterized. 2136357 0 29 gly glycoprotein 108:119 arg1 human and bovine uromodulin human and bovine uromodulin PUBTATOR uromodulin 281567 Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein). 2136357 0 29 gly glycoprotein 108:119 arg1 Tamm-Horsfall glycoprotein Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein). 2136357 0 64 gly uromodulin 82:91 arg1 oligomannose oligosaccharides uromodulin oligomannose oligosaccharides PUBTATOR uromodulin 281567 Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine uromodulin (Tamm-Horsfall glycoprotein). 23602662 1 30 gly glycoprotein 139:150 arg1 CD200 CD200 PUBTATOR CD200 4345 CD200 is a widely distributed membrane glycoprotein that regulates myeloid cell activity through its interaction with an inhibitory receptor (CD200R). 2943741 5 18 gly beta 1066:1069 arg1 NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAcOH, Gal beta 1----4GlcNAc beta 1----6(NeuNAc alpha 2----3)Gal beta 1----3)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6(NeuNAc alpha 2----3Gal beta 1----3)GalNAcOH Gal beta 1 NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAcOH, Gal beta 1----4GlcNAc beta 1----6(NeuNAc alpha 2----3)Gal beta 1----3)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6(NeuNAc alpha 2----3Gal beta 1----3)GalNAcOH OGER Gal beta 1 On the other hand, oligosaccharides from HL-60 and HSB-2 cells were found to be NeuNAc alpha 2----3Gal beta 1----3GalNAcOH, NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAcOH, Gal beta 1----4GlcNAc beta 1----6(NeuNAc alpha 2----3)Gal beta 1----3)GalNAcOH, and NeuNAc alpha 2----3Gal beta 1----4GlcNAc beta 1----6(NeuNAc alpha 2----3Gal beta 1----3)GalNAcOH. 27313224 5 62 gly N-glycosylation 939:953 arg1 SV2C SV2C PUBTATOR SV2C 22987 Here, we expressed human SV2C-LD4 fused to human IgG-Fc in prokaryotic and eukaryotic expression systems to analyse the effect of N-glycosylation of SV2C on the interaction with BoNT/A1. 1421757 1 30 gly glycoprotein 121:132 arg1 The human transferrin receptor The human transferrin receptor PUBTATOR transferrin receptor 7018 The human transferrin receptor is a glycoprotein containing three N-linked and one O-linked glycosylation sites. 17157876 8 22 gly subunit 1816:1822 arg1 the central leucine-rich repeat tandem CPN2 subunit the central leucine-rich repeat tandem PUBTATOR CPN2 subunit 1370 In tetrameric CPN, each CPN1 subunit might interact with the central leucine-rich repeat tandem of the cognate CPN2 subunit via a unique hydrophobic surface patch wrapping around the catalytic domain-TT interface, exposing the two active centers. 9677334 11 70 gly TPO 1513:1515 arg1 specific determinants TPO specific determinants PUBTATOR TPO 7066 Moreover we found that the Arg10 and Arg17 residues of TPO seem to be specific determinants for TPO/c-Mpl recognition. 3567160 0 6 gly G 110:110 arg1 the N-linked oligosaccharides human normal and pathological immunoglobulin G the N-linked oligosaccharides Cterm human normal and pathological immunoglobulin G Comparative structural study of the N-linked oligosaccharides of human normal and pathological immunoglobulin G. 15628971 6 51 gly attached 1280:1287 arg1 C4ST-1 AND N-linked oligosaccharides C4ST-1 N-linked oligosaccharides PUBTATOR C4ST-1 314694 These observations strongly suggest that N-linked oligosaccharides attached to C4ST-1 contribute to the production and stability of the active form of C4ST-1. 6833285 0 11 gly 2HS-glycoprotein 54:69 arg1 human plasma alpha 2HS-glycoprotein human plasma alpha 2HS-glycoprotein PUBTATOR alpha 2HS-glycoprotein 197 Characterization of the B-chain of human plasma alpha 2HS-glycoprotein. 18815274 9 9 gly glycosylated 1250:1261 arg1 SV2A SV2A PUBTATOR SV2A 64051 Together, the data reported here demonstrate that glycosylated SV2A and SV2B act in conjunction with gangliosides to mediate the entry of BoNT/E into neurons. 2059624 6 31 part_of r-HPg 1628:1632 arg1 N289 r-HPg N289 Cterm SpecificSite r-HPg P00747 N289 At an intermediate time period window, of 20-60 h, p.i., a mixture of complex-type oligosaccharides, totaling approximately 77% of the glycans, with various levels of branching and outer arm completion, and high-mannose type of oligosaccharides, totaling approximately 23% of the glycans, was assembled on N289 of the r-HPg produced. 2049076 8 22 gly carries 1210:1216 arg1 natural IFN-alpha 2 AND O-linked carbohydrates natural IFN-alpha 2 Thr-106 O-linked carbohydrates PUBTATOR IFN-alpha 2 3440 Thr-106 These results suggested that Thr-106 of natural IFN-alpha 2 carries O-linked carbohydrates. 15750791 3 4 gly glycosylated 422:433 arg1 Edg-1/S1P1 Edg-1/S1P1 PUBTATOR S1P1 1901 Our recent novel studies established that Edg-1/S1P1 is glycosylated in its N-terminal extracellular portion and further identified the specific glycosylation site as asparagine 30. 8999954 2 6 gly modified 280:287 arg1 eukaryotic RNA polymerase II AND Ser(Thr)-O-linked N-acetylglucosamine moieties eukaryotic RNA polymerase II Ser(Thr)-O-linked N-acetylglucosamine moieties OGER RNA polymerase II Many transcription factors and eukaryotic RNA polymerase II itself are also dynamically modified by Ser(Thr)-O-linked N-acetylglucosamine moieties (O-GlcNAc). 8999954 2 6 gly modified 280:287 arg3 eukaryotic RNA polymerase II AND O-GlcNAc eukaryotic RNA polymerase II O-GlcNAc OGER RNA polymerase II Many transcription factors and eukaryotic RNA polymerase II itself are also dynamically modified by Ser(Thr)-O-linked N-acetylglucosamine moieties (O-GlcNAc). 10413465 6 23 part_of apoE4 1298:1302 arg1 position 158 apoE4 position 158 PUBTATOR SpecificSite apoE4 348 cysteine at position 158 In contrast, introduction of cysteine at position 158 of apoE4 (Arg112, Cys158) increased the SDS-stable binding of apoE to Abeta to the levels similar to those observed in apoE2. 11595658 0 38 gly N-glycosylation 0:14 arg1 CRF receptor type 1 CRF receptor type 1 PUBTATOR CRF receptor type 1 1394 N-glycosylation of CRF receptor type 1 is important for its ligand-specific interaction. 18642129 12 83 gly O-glycosylated 1665:1678 arg1 FVII FVII OGER FVII P08709 Last, MS and MS/MS analysis revealed that FVII is O-glycosylated on the light chain at position Ser(60) and Ser(52) which are modified by oligosaccharide structures such as fucose and Glc(Xyl)(0-1-2), respectively. 18642129 12 83 gly O-glycosylated 1665:1678 arg1 the light chain the light chain OGER chain P08709 Last, MS and MS/MS analysis revealed that FVII is O-glycosylated on the light chain at position Ser(60) and Ser(52) which are modified by oligosaccharide structures such as fucose and Glc(Xyl)(0-1-2), respectively. 23959878 6 7 gly deglycosylated 924:937 arg1 native and deglycosylated hIDUA native and deglycosylated hIDUA PUBTATOR hIDUA 3425 The kinetics of native and deglycosylated hIDUA suggested that the N-glycan is also involved in catalytic processes. 1457969 4 89 gly liberated 648:656 arg1 intact hTSH beta AND The oligosaccharides intact hTSH beta The oligosaccharides PUBTATOR hTSH beta 7252 The oligosaccharides were liberated from hTSH alpha glycopeptides and from intact hTSH beta by hydrazinolysis, and were fractionated as alditols by anion-exchange and ion-suppression amine-adsorption HPLC preparatory to structural analysis. 21138434 6 23 gly N-glycosylation 1402:1416 arg1 the h5-HT3B subunit the h5-HT3B subunit Cterm h5-HT3B subunit 9177 The present study has identified utilised N-glycosylation sites of the h5-HT3B subunit and demonstrated that they promote subunit expression in the cell membrane; a prerequisite for 5-HT(3) receptor function. 8942648 0 22 part_of ser1 87:90 arg1 Peptide Peptide ser1 OGER AminoAcid Peptide ser1 Peptide, disulfide, and glycosylation mapping of recombinant human thrombopoietin from ser1 to Arg246. 8702840 3 46 gly modified 469:476 arg3 NF-L AND O-GlcNAc NF-L O-GlcNAc PUBTATOR NF-L 4747 NF-M and NF-L are known to be modified by O-linked N-acetylglucosamine (O-GlcNAc) (Dong, D. L.-Y., Xu, Z.-S., Chevrier, M. R., Cotter, R. J., Cleveland, D. W., and Hart, G. W. (1993) J. Biol. 8702840 3 46 gly modified 469:476 arg3 NF-M AND O-GlcNAc NF-M O-GlcNAc PUBTATOR NF-M 4741 NF-M and NF-L are known to be modified by O-linked N-acetylglucosamine (O-GlcNAc) (Dong, D. L.-Y., Xu, Z.-S., Chevrier, M. R., Cotter, R. J., Cleveland, D. W., and Hart, G. W. (1993) J. Biol. 8702840 3 46 gly modified 469:476 arg3 NF-L AND O-linked N-acetylglucosamine NF-L O-linked N-acetylglucosamine PUBTATOR NF-L 4747 NF-M and NF-L are known to be modified by O-linked N-acetylglucosamine (O-GlcNAc) (Dong, D. L.-Y., Xu, Z.-S., Chevrier, M. R., Cotter, R. J., Cleveland, D. W., and Hart, G. W. (1993) J. Biol. 8702840 3 46 gly modified 469:476 arg3 NF-M AND O-linked N-acetylglucosamine NF-M O-linked N-acetylglucosamine PUBTATOR NF-M 4741 NF-M and NF-L are known to be modified by O-linked N-acetylglucosamine (O-GlcNAc) (Dong, D. L.-Y., Xu, Z.-S., Chevrier, M. R., Cotter, R. J., Cleveland, D. W., and Hart, G. W. (1993) J. Biol. 7642555 0 52 gly glycosylated 9:20 arg1 c-Myc c-Myc PUBTATOR c-Myc 4609 c-Myc is glycosylated at threonine 58, a known phosphorylation site and a mutational hot spot in lymphomas. 3651384 2 13 gly glycosylated 253:264 arg1 SGP-2 SGP-2 PUBTATOR SGP-2 24854 Pulse-chase labeling shows that SGP-2 is synthesized as a cotranslationally glycosylated 64-kDa precursor that is modified to a negatively charged 73-kDa form before intracellular cleavage to the mature 47- and 34-kDa subunits. 16274239 1 57 part_of receptor 186:193 arg1 579 epidermal growth factor receptor N(579) PUBTATOR SpecificSite epidermal growth factor receptor 13649 N(579) We have investigated functional effects of glycosylation at N(579) of the epidermal growth factor receptor (EGFR). 2668275 12 14 gly contain 1566:1572 arg1 STS AND mannose 6-phosphate residues STS mannose 6-phosphate residues Cterm STS 412 In spite of its similarity with these two lysosomal sulfatases, STS does not contain mannose 6-phosphate residues and is transported to lysosomes by a mannose 6-phosphate receptor-independent mechanism. 24226769 0 65 gly glycosylates 28:39 arg1 Notch Notch PUBTATOR Notch 100037842 The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality. 8962717 0 56 gly glycosylation 16:28 arg1 the epidermal growth factor receptor the epidermal growth factor receptor PUBTATOR epidermal growth factor receptor P00533 Analysis of the glycosylation patterns of the extracellular domain of the epidermal growth factor receptor expressed in Chinese hamster ovary fibroblasts. 1371281 0 54 gly glycosylation 42:54 arg1 human cytokeratin 8 and 18 human cytokeratin 8 and 18 PUBTATOR cytokeratin 8 3856 Characterization and dynamics of O-linked glycosylation of human cytokeratin 8 and 18. 16679516 10 90 gly hTg-CS 1570:1575 arg1 the chondroitin 6-sulfate oligosaccharide unit hTg the chondroitin 6-sulfate oligosaccharide unit OGER hTg P01266 Furthermore, the chondroitin 6-sulfate oligosaccharide unit of hTg-CS protected peptide bond Lys2714-Gly2715 from proteolysis, during the limited digestion of hTg-CS with trypsin. 17924658 0 75 gly glycosylation 65:77 arg1 recombinant human gamma-glutamyltranspeptidase recombinant human gamma-glutamyltranspeptidase PUBTATOR gamma-glutamyltranspeptidase 102724197 Kinetic characterization and identification of the acylation and glycosylation sites of recombinant human gamma-glutamyltranspeptidase. 2226797 1 24 gly variant 217:223 arg1 The carbohydrate structures tissue plasminogen activator variant The carbohydrate structures PUBTATOR tissue plasminogen activator variant P00750 The carbohydrate structures of a genetically engineered human tissue plasminogen activator variant bearing a single N-glycosylation site at Asn 448 are reported. 7538124 0 75 gly glycosylation 46:58 arg1 human keratin 18 human keratin 18 PUBTATOR keratin 18 3875 Identification and mutational analysis of the glycosylation sites of human keratin 18. 2065054 2 58 part_of r-HPg 1153:1157 arg1 Asn289 HPg Asn289 Cterm AminoAcid HPg 5340 Asn289 In order to investigate whether a more general capability of lepidopteran insect cells to produce complex oligosaccharide existed, and to identify the chemical nature of the types of oligosaccharides that such insect cells were able to assemble, we have infected Mamestra brassicae (IZD-MBO503) cells for 48 h with a recombinant (r) baculovirus containing the [R561E]human plasminogen (HPg) cDNA and characterized the nature of the glycopeptidase F (GF) released N-linked oligosaccharides contained on Asn289 of the r-HPg expressed by these cells. 24342833 6 41 part_of β-catenin 956:964 arg1 Serine 23 -catenin Serine 23 PUBTATOR SpecificSite -catenin 1499 Serine 23 RESULTS: Serine 23 of β-catenin was determined as a site for O-GlcNAc modification which regulated its subcellular distribution, its interactions with cellular partners and consequently its transcriptional activity. 26271046 5 87 gly N-glycans 903:911 arg1 GluA2 GluA2 N-glycans PUBTATOR GluA2 2891 Moreover, it is thought that other N-glycans on GluA2 also have potential roles in the regulation of AMPAR functions. 17322565 1 15 gly glycosylation 196:208 arg1 human endothelial lipase human endothelial lipase PUBTATOR endothelial lipase 9388 We previously identified that four of five putative N-linked glycosylation sites of human endothelial lipase (EL) are utilized and suggested that the substitution of asparagine-116 (Asn-116) with alanine (Ala) (N116A) increased the hydrolytic activity of EL. 17322565 1 15 gly glycosylation 196:208 arg1 EL EL PUBTATOR EL 9388 We previously identified that four of five putative N-linked glycosylation sites of human endothelial lipase (EL) are utilized and suggested that the substitution of asparagine-116 (Asn-116) with alanine (Ala) (N116A) increased the hydrolytic activity of EL. 10206894 1 11 gly Zn-alpha2-glycoprotein 81:102 arg1 ZAG ZAG PUBTATOR ZAG 563 Zn-alpha2-glycoprotein (ZAG) is a soluble protein that is present in serum and other body fluids. 10206894 1 11 gly Zn-alpha2-glycoprotein 81:102 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Zn-alpha2-glycoprotein (ZAG) is a soluble protein that is present in serum and other body fluids. 19413349 8 90 gly deglycosylation 1378:1392 arg1 melanopsin function melanopsin function PUBTATOR melanopsin 192223 Finally, neither in vivo N-linked deglycosylation nor mutations of the two N-linked glycosylation sites significantly affected melanopsin function measured by Fos induction after light stimulation. 18702514 3 29 gly unglycosylated 458:471 arg1 human pancreatic lipase-related protein 2 human pancreatic lipase-related protein 2 PUBTATOR pancreatic lipase-related protein 2 5408 Here we report two crystal structures of wild-type and unglycosylated human pancreatic lipase-related protein 2 (HPLRP2) with the lid in an open conformation in the absence of amphiphiles. 18702514 3 29 gly unglycosylated 458:471 arg1 HPLRP2 HPLRP2 OGER HPLRP2 P54318 Here we report two crystal structures of wild-type and unglycosylated human pancreatic lipase-related protein 2 (HPLRP2) with the lid in an open conformation in the absence of amphiphiles. 1388166 2 48 gly TFPI 334:337 arg1 the oligosaccharides TFPI the oligosaccharides PUBTATOR TFPI 7035 We have determined that greater than 70% of the oligosaccharides on recombinant TFPI expressed in 293 cells terminate with the sequence SO4-4GalNAc beta 1, 4GlcNAc beta 1, 2Man alpha. 1388166 2 75 gly oligosaccharides 302:317 arg1 recombinant TFPI TFPI oligosaccharides PUBTATOR TFPI 7035 We have determined that greater than 70% of the oligosaccharides on recombinant TFPI expressed in 293 cells terminate with the sequence SO4-4GalNAc beta 1, 4GlcNAc beta 1, 2Man alpha. 23167757 7 72 gly glycosylation 1257:1269 arg1 PDIA2 protein-protein interactions PDIA2 protein-protein interactions PUBTATOR PDIA2 64714 Nevertheless, in HeLa cells, both wild-type and N127/284/516Q mutant PDIA2 proteins localize to the ER, but not the ER-Golgi intermediate compartment, suggesting that glycosylation is important for PDIA2 protein-protein interactions but not subcellular localization. 2059624 4 13 part_of r-HPg 947:951 arg1 N289 r-HPg N289 Cterm SpecificSite r-HPg P00747 N289 At the earliest postinfection (p.i.) time period studied, i.e., 0-20 h, virtually all (96%) of the oligosaccharides released with glycopeptidase F from N289 of the expressed r-HPg were of the high-mannose type and comprised nearly the full range of such structures, containing 3-9 mannose units. 10889209 8 6 gly N-glycosylation 1526:1540 arg1 ROMK1 ROMK1 PUBTATOR ROMK1 3758 Thus, N-glycosylation of GIRK1 at Asn(119) does not appear to affect its physical association with GIRK4, the routing of the heteromer to the cell surface, or heteromeric channel function, unlike the dramatic functional effects of N-glycosylation of ROMK1 at Asn(117) (Schwalbe, R. A., Wang, Z., Wible, B. A., and Brown, A. M. (1995) J. Biol. 10889209 8 14 gly N-glycosylation 1301:1315 arg1 GIRK1 GIRK1 PUBTATOR GIRK1 3760 Thus, N-glycosylation of GIRK1 at Asn(119) does not appear to affect its physical association with GIRK4, the routing of the heteromer to the cell surface, or heteromeric channel function, unlike the dramatic functional effects of N-glycosylation of ROMK1 at Asn(117) (Schwalbe, R. A., Wang, Z., Wible, B. A., and Brown, A. M. (1995) J. Biol. 20622017 0 46 gly glycosylation 26:38 arg1 renal and hepatic γ-glutamyl transpeptidase renal and hepatic γ-glutamyl transpeptidase PUBTATOR -glutamyl transpeptidase 102724197 Analysis of site-specific glycosylation of renal and hepatic γ-glutamyl transpeptidase from normal human tissue. 15702487 8 22 gly Glycosylation 1462:1474 arg1 alpha 5 beta 1 subunits alpha 5 beta 1 subunits OGER subunits P08648 Glycosylation of alpha 5 beta 1 subunits of human integrin was studied to test the methodology. 24121512 6 21 part_of cdAE1 1228:1232 arg1 55-356 cdAE1 55-356 Cterm SpecificSite cdAE1 6521 residues 55-356 A 2.1-Å resolution crystal structure of cdΔ54AE1 (residues 55-356 of cdAE1) lacking the amino-terminal and carboxyl-terminal disordered regions, produced at physiological pH, revealed an extensive hydrogen-bonded network involving Arg(283) and Glu(85). 12526797 2 13 part_of ICAM-1 366:371 arg1 Glu-34 ICAM-1 Glu-34 PUBTATOR SpecificSite ICAM-1 3383 Glu-34 The I domain Mg2+ directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain residue Glu-241 enables a critical salt bridge. 17715132 5 1 gly glycosylated 694:705 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We show for the first time that Pannexin1 is glycosylated at Asn-254 and that this residue is important for plasma membrane targeting. 1421756 9 78 part_of contains 1449:1456 arg1 s-TfR AND Thr104 TfR Thr104 PUBTATOR AminoAcid TfR 7037 Thr104 From human serum we purified the cleaved, soluble form of the TfR (s-TfR), which contains Thr104, but lacks Thr96. 14764083 1 6 gly glycoprotein 150:161 arg1 Lactoferrin Lactoferrin PUBTATOR Lactoferrin 280846 Lactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. 24692546 4 1 gly deglycosylated 650:663 arg1 deglycosylated FSH deglycosylated FSH OGER FSH It also predicts that, upon dissociation of the FSHR trimer into monomers, the binding of glycosylated FSH, but not deglycosylated FSH, would increase 3-fold, and that the dissociated monomers would in turn enhance FSHR binding and signaling activities by 3-fold. 24692546 4 29 gly glycosylated 624:635 arg1 glycosylated FSH glycosylated FSH OGER FSH It also predicts that, upon dissociation of the FSHR trimer into monomers, the binding of glycosylated FSH, but not deglycosylated FSH, would increase 3-fold, and that the dissociated monomers would in turn enhance FSHR binding and signaling activities by 3-fold. 10715549 4 46 part_of LHbeta 1064:1069 arg1 the Asn(13) LHbeta the Asn(13) PUBTATOR SpecificSite LHbeta 3972 Asn(13) No aggregation was seen when N-linked oligosaccharides were attached to the Asn(13) of LHbeta. 3264725 7 19 gly N-glycosylated 1475:1488 arg1 human plasma factor VIIa human plasma factor VIIa Cterm factor VIIa Asparagine residues 145 and 322 were found to be fully N-glycosylated in human plasma factor VIIa. 20805301 5 12 gly glycosylation 689:701 arg1 CYP2W1 CYP2W1 PUBTATOR CYP2W1 54905 Bioinformatic analysis identified Asn177 as the only possible glycosylation site of CYP2W1, which was supported by the inability of an N177A mutant to be glycosylated in HEK 293 cells. 8344280 0 63 gly glycoprotein 50:61 arg1 human interleukin 2 glycoprotein variants human interleukin 2 glycoprotein variants PUBTATOR interleukin 2 glycoprotein 3558 Biosynthesis and secretion of human interleukin 2 glycoprotein variants from baculovirus-infected Sf21 cells. 7525874 1 61 gly protein 163:169 arg1 The carbohydrate structures beta-trace protein The carbohydrate structures PUBTATOR beta-trace protein 5730 The carbohydrate structures of beta-trace protein from human cerebrospinal fluid have been elucidated. 8323299 7 1 part_of lamp-1 1218:1223 arg1 residues 167 to 190 lamp-1 residues 167 to 190 PUBTATOR SpecificSite lamp-1 3916 residues 167 Circular dichroism and nuclear magnetic resonance spectroscopy was used for the structural characterization of a synthetic peptide corresponding to residues 167 to 190 of lamp-1. 8798614 1 68 gly glycoprotein 176:187 arg1 CD59 CD59 PUBTATOR CD59 966 CD59 is a glycosylphosphatidylinositol-anchored membrane glycoprotein that serves as the principle cellular inhibitor of the C5b-9 membrane attack complex (MAC) of human complement. 1898343 1 24 gly glycoprotein 93:104 arg1 Human pancreatic elastase 1 Human pancreatic elastase 1 PUBTATOR Human pancreatic elastase 1 1990 Human pancreatic elastase 1 (E1) is a glycoprotein containing two potential N-glycosylation sites, one of which carries a carbohydrate moiety [Wendorf, Geyer, Sziegoleit & Linder (1989) FEBS Lett. 11738084 6 11 gly glycosylated 804:815 arg1 glycosylated albumin variants glycosylated albumin variants OGER albumin variants P02768 The structure is principally the same as that of glycans bound to two other types of glycosylated albumin variants. 1371281 6 16 gly CK18 879:882 arg1 N-acetylglucosamine/protein molecule CK18 N-acetylglucosamine/protein molecule PUBTATOR CK18 3875 Using chemical analysis, the stoichiometry of glycosylation was found to be 1.5 and 2 molecules of N-acetylglucosamine/protein molecule of CK8 and CK18, respectively. 1371281 6 33 gly CK8 871:873 arg1 N-acetylglucosamine/protein molecule CK8 N-acetylglucosamine/protein molecule PUBTATOR CK8 3856 Using chemical analysis, the stoichiometry of glycosylation was found to be 1.5 and 2 molecules of N-acetylglucosamine/protein molecule of CK8 and CK18, respectively. 14707133 1 28 gly glypican-1 193:202 arg1 a glycosylphosphatidylinositol-linked heparan sulfate proteoglycan glypican-1 a glycosylphosphatidylinositol-linked heparan sulfate proteoglycan PUBTATOR glypican-1 58920 The core protein of glypican-1, a glycosylphosphatidylinositol-linked heparan sulfate proteoglycan, can bind Cu(II) or Zn(II) ions and undergo S-nitrosylation in the presence of nitric oxide. 21932778 7 35 gly N-glycosylated 1267:1280 arg1 N-glycosylated glypican-1 N-glycosylated glypican-1 PUBTATOR N-glycosylated glypican-1 2817 N-Glycosylation mutants and N-deglycosylated glypican-1 had far-UV circular dichroism and fluorescence emission spectra that were highly similar to those of N-glycosylated glypican-1. 21932778 7 68 gly N-deglycosylated 1138:1153 arg1 N-deglycosylated glypican-1 N-deglycosylated glypican-1 PUBTATOR N-deglycosylated glypican-1 2817 N-Glycosylation mutants and N-deglycosylated glypican-1 had far-UV circular dichroism and fluorescence emission spectra that were highly similar to those of N-glycosylated glypican-1. 15014436 2 55 gly TSPs 242:245 arg1 the calcium-binding type 3 (T3) repeats TSPs the calcium-binding type 3 (T3) repeats Cterm TSPs 7057 The most highly conserved region of all TSPs are the calcium-binding type 3 (T3) repeats and the C-terminal globular domain (CTD). 17591618 2 102 gly glycoprotein 327:338 arg1 CFH CFH PUBTATOR CFH P08603 CFH is a 155-kDa glycoprotein containing nine potential N-glycosylation sites. 26271046 3 95 gly GluA2 669:673 arg1 N-linked glycans GluA2 N-linked glycans PUBTATOR GluA2 2891 To date, a unique trisaccharide (HSO3-3GlcAβ1-3Galβ1-4GlcNAc), human natural killer-1 (HNK-1) carbohydrate, was found expressed specifically on N-linked glycans of GluA2 and regulated the cell surface expression of AMPAR and the spine maturation process. 3571235 5 26 part_of C 741:741 arg1 residues 49-88 glycophorin C residues 49-88 PUBTATOR SpecificSite glycophorin C 2995 residues 49-88 The amino acid sequence of the hydrophobic domain (residues 49-88) of glycophorin C, that was also determined, agreed completely with the structure recently deduced from cDNA sequencing. 14699159 9 69 gly glycosylation 1805:1817 arg1 p90ATF6 p90ATF6 Cterm p90ATF6 22926 Because accumulation of underglycosylated proteins in the ER is a potent inducer for the UPR, these studies uncover a novel mechanism whereby the glycosylation status of p90ATF6 can serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the UPR. 7629073 7 34 part_of Thr411 934:939 arg1 clone 4 clone 4 Thr411 PUBTATOR AminoAcid clone 4 22116 Thr411, Leu468, and Ser592 Their overlapping sequences (from amino acid 152) are identical, except for three point mutations that result in three amino acid differences in the catalytic domain of the enzyme (Thr411, Leu468, and Ser592 in clone 4 to Met411, Phe468, and Phe592 in clone 16, respectively). 7629073 7 37 part_of Leu468 942:947 arg1 clone 4 clone 4 Leu468 PUBTATOR AminoAcid clone 4 22116 Thr411, Leu468, and Ser592 Their overlapping sequences (from amino acid 152) are identical, except for three point mutations that result in three amino acid differences in the catalytic domain of the enzyme (Thr411, Leu468, and Ser592 in clone 4 to Met411, Phe468, and Phe592 in clone 16, respectively). 7629073 7 100 part_of Ser592 954:959 arg1 clone 4 clone 4 Ser592 PUBTATOR AminoAcid clone 4 22116 Thr411, Leu468, and Ser592 Their overlapping sequences (from amino acid 152) are identical, except for three point mutations that result in three amino acid differences in the catalytic domain of the enzyme (Thr411, Leu468, and Ser592 in clone 4 to Met411, Phe468, and Phe592 in clone 16, respectively). 15869464 1 85 gly glycoprotein 258:269 arg1 OPN OPN PUBTATOR OPN 6696 OPN (osteopontin) is an integrin-binding highly phosphorylated glycoprotein, recognized as a key molecule in a multitude of biological processes such as bone mineralization, cancer metastasis, cell-mediated immune response, inflammation and cell survival. 14711516 4 4 gly N-glycosylation 752:766 arg1 recombinant chicken Thy-1 recombinant chicken Thy-1 PUBTATOR Thy-1 P04216 The disulfide linkage pattern and glycoform distribution on each N-glycosylation site of recombinant chicken Thy-1 from both cell lines were determined by a combination of amino-terminal sequencing and mass spectrometry. 7681597 4 25 gly containing 827:836 arg1 GPA-2 AND two nonconsecutive GalNAc-Ser/Thr residues GPA-2 the glycopeptide two nonconsecutive GalNAc-Ser/Thr residues OGER GPA-2 P02724 glycopeptide The Tn antigenicity, as assayed by the binding to a monoclonal anti-Tn antibody (MLS 128), was found exclusively in the glycopeptides including three (cluster I) or four (cluster II) consecutive residues of GalNAc-Ser/Thr, whereas the glycopeptide (GPA-2) containing two nonconsecutive GalNAc-Ser/Thr residues had practically no Tn antigenicity. 22688517 6 24 gly glycosylation 1145:1157 arg1 recombinant human LOX-1 recombinant human LOX-1 PUBTATOR LOX-1 4973 With this approach, one potential glycosylation site of recombinant human LOX-1 on Asn(139) is readily identified and found to carry heterogeneous complex type N-glycans. 22688517 6 30 gly carry 1238:1242 arg1 recombinant human LOX-1 AND heterogeneous complex type N-glycans recombinant human LOX-1 one potential glycosylation site heterogeneous complex type N-glycans PUBTATOR LOX-1 4973 site With this approach, one potential glycosylation site of recombinant human LOX-1 on Asn(139) is readily identified and found to carry heterogeneous complex type N-glycans. 9405696 3 2 gly glycosylated 536:547 arg1 BCNG-1 BCNG-1 PUBTATOR BCNG-1 15165 BCNG-1 is expressed exclusively in the brain, as a glycosylated protein of approximately 132 kDa. 8702538 8 96 gly glycoprotein 1372:1383 arg1 myelin-associated glycoprotein myelin-associated glycoprotein OGER myelin-associated glycoprotein P20916 Site-directed mutagenesis of similar NC(T/S) motifs in the first or second Ig domains of the I-type lectins myelin-associated glycoprotein, and sialoadhesin did not disrupt their ability to mediate sialic acid binding. 6725284 1 40 gly galactoglycoprotein 138:156 arg1 Human plasma galactoglycoprotein Human plasma galactoglycoprotein PUBTATOR galactoglycoprotein 6693 Human plasma galactoglycoprotein (Mr = 81,000) which was recently identified and characterized (Schmid, K., Mao, S. K. Y., Kimura, A., Hayashi, S., and Binette , J. P. (1980) J. Biol. 12731890 3 50 gly glycosylated 564:575 arg1 the naturally expressed human EGF receptor the naturally expressed human EGF receptor PUBTATOR EGF receptor 1956 Our data show that the naturally expressed human EGF receptor is fully glycosylated on eight of the 11 canonical sites; two of the sites are not glycosylated, and one is partially glycosylated, a pattern of site-usage similar but not identical to those reported for the recombinant human EGF receptor heterologously expressed in Chinese hamster ovary cells. 2015821 6 13 gly carries 1264:1270 arg1 alpha 1-antichymotrypsin AND four oligosaccharide side chains alpha 1-antichymotrypsin four oligosaccharide side chains PUBTATOR alpha 1-antichymotrypsin 12 From these experiments it is concluded that alpha 1-antichymotrypsin carries four oligosaccharide side chains. 3179269 1 60 gly erythropoietin 165:178 arg1 the N-linked oligosaccharides erythropoietin the N-linked oligosaccharides PUBTATOR erythropoietin 2056 The structures of the N-linked oligosaccharides of the urinary erythropoietin (u-EPO) purified from urine of aplastic anemic patients were analyzed and compared with those for recombinant erythropoietin (r-EPO) prepared with baby hamster kidney (BHK) cells. 11180632 1 15 gly residues 140:147 arg1 saposin B saposin B residues Cterm saposin B The specific sugar residues and their linkages in the oligosaccharides from pig kidney and human urine cerebroside sulfate activator proteins (saposin B), although previously hypothesized, have been unambiguously characterized. 3356193 4 1 gly plasminogen 475:485 arg1 the N-glycan plasminogen the N-glycan OGER plasminogen P00747 In the N-glycan of human plasminogen the two antennae are sialylated with N-acetylneuraminic acid (NeuAc), whereas in the bovine counterpart both branches carry significant amounts of N-glycolylneuraminic acid (NeuGc). 28450392 3 81 gly NSL3 770:773 arg1 O-GlcNAcylation NSL3 O-GlcNAcylation PUBTATOR NSL3 55683 Here, we demonstrate that OGT1 regulates the activity of the NSL complex by mainly acetylating histone H4 Lys-16, Lys-5, and Lys-8 via O-GlcNAcylation and stabilization of the NSL complex subunit NSL3. 8615697 13 64 part_of residues 2281:2288 arg1 hTG hTG residues OGER SpecificSite hTG P01266 asparagine residues 91, 477, 1849, and 2102 Only four of the 20 putative sites the sequence of hTG, at asparagine residues 91, 477, 1849, and 2102 were not represented in the purified glycopeptide population and are presumed to escape significant glycosylation. 21550978 7 38 part_of hPAR 1516:1519 arg1 Asn(250) hPAR(1) Asn(250) PUBTATOR SpecificSite hPAR(1) 2149 Asn(250) Removing these N-linked glycosylation sequons affected hPAR(1) cell surface expression to varying degrees, and N-linked glycosylation at extracellular loop 2 (especially Asn(250)) of hPAR(1) is essential for optimal receptor cell surface expression and receptor stability. 14573609 5 2 gly glycosylation 697:709 arg1 heparanase heparanase PUBTATOR heparanase 10855 Treatment with a glycosylation inhibitor demonstrated that glycosylation was not required for the activity of heparanase. 9054441 3 68 gly contain 308:314 arg1 Plasminogen 2 AND only an O-linked oligosaccharide Plasminogen 2 only an O-linked oligosaccharide OGER Plasminogen 2 P00747 Plasminogen 2 is known to contain only an O-linked oligosaccharide at Thr-345. 14970177 5 50 gly glycosylated 1036:1047 arg1 glycosylated hIL-6 glycosylated hIL-6 OGER hIL-6 P05231 As distinct from vIL-6, unglycosylated hIL-6 is as potent as glycosylated hIL-6 in stimulating B cell proliferation. 14970177 5 58 gly unglycosylated 999:1012 arg1 unglycosylated hIL-6 unglycosylated hIL-6 OGER hIL-6 P05231 As distinct from vIL-6, unglycosylated hIL-6 is as potent as glycosylated hIL-6 in stimulating B cell proliferation. 1872845 6 42 gly contains 933:940 arg1 vWF itself AND UEA-I reactive sugar chains vWF itself UEA-I reactive sugar chains PUBTATOR vWF 7450 Our results indicate that vWF itself contains UEA-I reactive sugar chains in its Asn-linked oligosaccharides. 3497198 4 35 part_of H-2Kb 676:680 arg1 Asn176 H-2Kb Asn176 PUBTATOR AminoAcid H-2Kb 14972 Asn176 Asn176 of both H-2Kk and H-2Kb contained the same ratio (2.8 to 1) of bi- to monosialylated chains. 3497198 4 45 part_of H-2Kk 666:670 arg1 Asn176 H-2Kk Asn176 PUBTATOR AminoAcid H-2Kk 14972 Asn176 Asn176 of both H-2Kk and H-2Kb contained the same ratio (2.8 to 1) of bi- to monosialylated chains. 15807535 9 4 gly glycosylated 1709:1720 arg1 glycosylated ABCG2 glycosylated ABCG2 PUBTATOR ABCG2 Q9UNQ0 Furthermore, in crude membrane preparations, neither the basal nor the prazosin-stimulated ( approximately 2-fold) ATPase activities of ABCG2 (N596Q) were affected compared to glycosylated ABCG2. 22187327 2 7 gly ICAM-5 309:314 arg1 the N-glycan structures ICAM-5 the N-glycan structures PUBTATOR ICAM-5 15898 Although we have determined the N-glycan structures of ICAM-5 in a previous report, their function is unknown. 27294781 1 50 gly glycosylated 376:387 arg1 glycosylated human SV2C glycosylated human SV2C PUBTATOR SV2C 22987 Here we report a 2.0-Å-resolution crystal structure of the BoNT/A1 receptor-binding domain in complex with its neuronal receptor, glycosylated human SV2C. 3056714 2 27 gly glycosylated 366:377 arg1 The CT29-LAP The CT29-LAP PUBTATOR CT29 57082 The CT29-LAP was synthesized in BHK cells as a heterogeneously glycosylated precursor that was tightly membrane associated. 12775711 1 37 gly glycoprotein 140:151 arg1 HCGP39 HCGP39 PUBTATOR HCGP39 1116 The 39-kDa human cartilage glycoprotein (HCGP39), a member of a novel family of chitinase-like lectins (Chilectins), is overexpressed in articular chondrocytes and certain cancers. 23376777 10 97 gly glycosylation 1744:1756 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 We propose that glycosylation is essential for the surface expression, stabilization, and bioactivity of KCC4. 8142896 0 20 gly receptor 102:109 arg1 the carbohydrate moieties interferon gamma receptor the carbohydrate moieties PUBTATOR interferon gamma receptor 3458 Structural analysis and localization of the carbohydrate moieties of a soluble human interferon gamma receptor produced in baculovirus-infected insect cells. 1421756 10 3 gly contains 1577:1584 arg1 the s-TfR AND an O-linked oligosaccharide the s-TfR an O-linked oligosaccharide PUBTATOR TfR 7037 The s-TfR was sensitive to O-glycanase and bound to Jacalin lectin, indicating that the s-TfR contains an O-linked oligosaccharide. 22187327 1 5 gly glycoprotein 196:207 arg1 Intercellular adhesion molecule-5 Intercellular adhesion molecule-5 PUBTATOR Intercellular adhesion molecule-5 15898 Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized type I membrane glycoprotein, and promotes dendritic filopodia formation. 4373463 0 4 gly immunoglobulin 44:57 arg1 the carbohydrate units IgA1 immunoglobulin the carbohydrate units PUBTATOR IgA1 immunoglobulin P01876 Structure of the carbohydrate units of IgA1 immunoglobulin. 8631363 8 73 gly 0-glycosylated 1262:1275 arg1 tumor necrosis factor-alpha tumor necrosis factor-alpha PUBTATOR tumor necrosis factor-alpha 7124 About 20% of tumor necrosis factor-alpha was found to be 0-glycosylated, based on the results of the sugar composition and structure analyses. 17082223 8 41 gly Bsep 1644:1647 arg1 one glycan Bsep one glycan PUBTATOR Bsep 83569 To determine whether a specific glycosylation site or the number of glycans was critical for protein stability, we studied the protein expression of combinations of N-glycan-deficient mutants and observed that Bsep with one glycan was considerably unstable compared with Bsep harboring two or more glycans. 11733580 0 5 gly O-glycans 11:19 arg1 CC chemokine receptor 5 CC chemokine receptor 5 O-glycans PUBTATOR CC chemokine receptor 5 1234 Sialylated O-glycans and sulfated tyrosines in the NH2-terminal domain of CC chemokine receptor 5 contribute to high affinity binding of chemokines. 8357534 2 39 part_of has 374:376 arg1 cathepsin G AND Asn-64 cathepsin G Asn-64 PUBTATOR SpecificSite cathepsin G 1511 Asn-64 Elastase has two N-glycosylation sites occupied (Asn-45 and Asn-144), whereas cathepsin G has only one (Asn-64). 21752865 4 61 gly N-glycosylated 857:870 arg1 BRI2 BRI2 PUBTATOR BRI2 9445 In support, bioinformatics analysis indicated that BRI2 bears the consensus sequence Asn-Thr-Ser (residues 170-173) and could be N-glycosylated at Asn170. 9852066 0 52 gly O-glycosylation 20:34 arg1 CGA79-439 CGA79-439 Cterm CGA79-439 1113 Phosphorylation and O-glycosylation sites of human chromogranin A (CGA79-439) from urine of patients with carcinoid tumors. 9852066 0 52 gly O-glycosylation 20:34 arg1 human chromogranin A human chromogranin A PUBTATOR chromogranin A 1113 Phosphorylation and O-glycosylation sites of human chromogranin A (CGA79-439) from urine of patients with carcinoid tumors. 19413349 0 37 gly deglycosylated 9:22 arg1 N-linked deglycosylated melanopsin N-linked deglycosylated melanopsin PUBTATOR melanopsin 192223 N-linked deglycosylated melanopsin retains its responsiveness to light. 18508581 3 1 gly glycosylation 497:509 arg1 EpCAM EpCAM PUBTATOR EpCAM 4072 We have uncovered differential glycosylation of EpCAM as a means to discriminate normal from malignant tissues. 21886772 8 18 gly N-glycosylation 1415:1429 arg1 FKRP homodimer FKRP homodimer PUBTATOR FKRP homodimer 79147 FKRP contains N-glycan of high mannose and/or hybrid type; however, FKRP N-glycosylation is not required for FKRP homodimer or multimer formation. 21886772 8 22 gly contains 1347:1354 arg1 FKRP AND N-glycan FKRP N-glycan PUBTATOR FKRP 79147 FKRP contains N-glycan of high mannose and/or hybrid type; however, FKRP N-glycosylation is not required for FKRP homodimer or multimer formation. 12545205 10 49 gly contained 1634:1642 arg1 N-cadherin AND complex type chains N-cadherin complex type chains PUBTATOR N-cadherin 1000 In addition, N-cadherin from WM9 (lymphomodus metastatic site) and A375 (solid tumor metastatic site) contained complex type chains with alpha2-3 sialic acid (positive reaction with Maackia amurensis agglutinin--MAA). 1991473 6 48 part_of hLH 817:819 arg1 Asn30 hLH beta Asn30 PUBTATOR AminoAcid hLH beta 3972 Asn30 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 1991473 6 60 part_of hLH 795:797 arg1 Asn52 hLH alpha Asn52 PUBTATOR AminoAcid hLH alpha 1081 Asn52 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 19017797 2 60 part_of FMS 482:484 arg1 D1-D3 FMS D1-D3 OGER SiteSequence FMS P07333 D1-D3 We report a 2.4 A crystal structure of M-CSF bound to the first 3 domains (D1-D3) of FMS. 1998667 1 47 gly glycoprotein 124:135 arg1 Factor XI Factor XI OGER Factor XI P03951 Factor XI is a plasma glycoprotein that participates in the blood coagulation cascade. 1456441 9 23 gly N-glycosylation 1336:1350 arg1 h-STF h-STF Cterm h-STF 7018 Di- and triantennary oligosaccharides were found to occur on each of the two N-glycosylation sites of h-STF (Asn413 and Asn611) in the ratio of approximately 85:15. 17286803 4 19 gly glycosylated 519:530 arg1 CLN3 CLN3 OGER CLN3 Q13286 Mutational analysis revealed that in COS7 cells, CLN3 is glycosylated at asparagine residues 71 and 85. 11168369 0 24 gly O-glycosylated 77:90 arg1 albumin Kénitra albumin Kénitra PUBTATOR albumin Kénitra 213 A nucleotide insertion and frameshift cause albumin Kénitra, an extended and O-glycosylated mutant of human serum albumin with two additional disulfide bridges. 22159084 3 5 gly glycosylated 324:335 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 It has been shown that SLC26A3 are glycosylated, with the attached carbohydrate being extracellular and perhaps modulating function. 17286803 3 61 gly glycosylation 351:363 arg1 CLN3 CLN3 OGER CLN3 Q13286 We now examined the role of glycosylation and the C-terminal CAAX motif in lysosomal transport of CLN3 in non-neuronal and neuronal cells. 21569239 0 76 gly glycosylated 35:46 arg1 F-spondin F-spondin PUBTATOR F-spondin 10418 The structure of the Ca²+-binding, glycosylated F-spondin domain of F-spondin - A C2-domain variant in an extracellular matrix protein. 7613477 1 31 gly glycosylation 289:301 arg1 human lecithin human lecithin PUBTATOR lecithin:cholesterol acyltransferase 3931 Site-specific structural characterization of the glycosylation of human lecithin:cholesterol acyltransferase (LCAT) was carried out using microbore reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC/ESIMS). 7613477 0 59 gly glycosylation 63:75 arg1 apolipoprotein D apolipoprotein D PUBTATOR apolipoprotein D 347 Site-specific detection and structural characterization of the glycosylation of human plasma proteins lecithin:cholesterol acyltransferase and apolipoprotein D using HPLC/electrospray mass spectrometry and sequential glycosidase digestion. 7613477 0 59 gly glycosylation 63:75 arg1 lecithin:cholesterol acyltransferase lecithin:cholesterol acyltransferase PUBTATOR lecithin:cholesterol acyltransferase 3931 Site-specific detection and structural characterization of the glycosylation of human plasma proteins lecithin:cholesterol acyltransferase and apolipoprotein D using HPLC/electrospray mass spectrometry and sequential glycosidase digestion. 24365146 0 30 gly glycosylation 9:21 arg1 the superoxide-producing NADPH oxidase Nox1 the superoxide-producing NADPH oxidase Nox1 PUBTATOR Nox1 27035 N-Linked glycosylation of the superoxide-producing NADPH oxidase Nox1. 9712881 2 46 part_of AE1 223:225 arg1 Glu681 AE1 Glu681 PUBTATOR AminoAcid AE1 6521 Glu681 Glu681 of human AE1 may form part of the anion translocation apparatus and the permeability barrier. 22159084 6 62 gly glycosylated 670:681 arg1 the mature glycosylated form form of SLC26A3 PUBTATOR form of SLC26A3 1811 Deglycosylation experiments with glycosidases indicated that the mature glycosylated form of SLC26A3 exists at the plasma membrane, and a putative large second extracellular loop contains all of the N-linked carbohydrates. 20511397 2 13 gly glycosylated/sialylated 523:545 arg1 plasma apoE plasma apoE PUBTATOR apoE 348 Cellular apoE and plasma apoE exist as multiple glycosylated and sialylated glycoforms with plasma apoE being less glycosylated/sialylated than cell-derived apoE. 11226831 0 23 gly Glycosylation 0:12 arg1 the murine estrogen receptor-alpha the murine estrogen receptor-alpha PUBTATOR estrogen receptor 13982 Glycosylation of the murine estrogen receptor-alpha. 8407981 3 30 gly glycosylation 303:315 arg1 arom P-450(arom) PUBTATOR P-450(arom) 55010 The core glycosylation of P-450(arom) was examined with two heterologous expression systems, cultured insect cells and in vitro translation system. 1472036 4 25 part_of Cys524 925:930 arg1 the other alpha-subunit subunit Cys524 OGER AminoAcid subunit 3643 Cys524 Since it has been shown that the extracellular domain of the insulin receptor has no free thiols and since no other sequences containing cysteine were found in these fractions, we conclude that Cys524 forms a disulfide bond to the Cys524 in the other alpha-subunit. 17711303 1 60 gly glycosylation 144:156 arg1 FLAG-hKOR FLAG-hKOR PUBTATOR hKOR 4986 We examined glycosylation of FLAG-hKOR expressed in CHO cells and determined its functional significance. 1388166 0 23 gly oligosaccharides 22:37 arg1 tissue factor pathway inhibitor tissue factor pathway inhibitor oligosaccharides PUBTATOR tissue factor pathway inhibitor 7035 The asparagine-linked oligosaccharides on tissue factor pathway inhibitor terminate with SO4-4GalNAc beta 1, 4GlcNAc beta 1,2 Mana alpha. 1997323 11 88 gly antenna 1222:1228 arg1 rTf-1 rTf-1 antenna PUBTATOR rTf-1 Q92541 However, the alpha-1,3-Man-linked antenna in rTf-1 as well as rTf-2 had the sequence: Neu5Ac(alpha 2-3)Gal(beta 1-3)[Neu5Ac(alpha 2-6)]GlcNAc(beta 1-2)Man. 1997323 11 88 gly antenna 1222:1228 arg1 rTf-2 rTf-2 antenna OGER rTf-2 Q3T1J8 However, the alpha-1,3-Man-linked antenna in rTf-1 as well as rTf-2 had the sequence: Neu5Ac(alpha 2-3)Gal(beta 1-3)[Neu5Ac(alpha 2-6)]GlcNAc(beta 1-2)Man. 15254193 8 44 gly N-glycosylated 1164:1177 arg1 Most murine IFN-alpha Most murine IFN-alpha PUBTATOR IFN-alpha 111654 Most murine IFN-alpha turned out to be N-glycosylated. 15807535 0 41 gly glycosylation 9:21 arg1 the human ABC transporter ABCG2 the human ABC transporter ABCG2 PUBTATOR ABCG2 9429 N-Linked glycosylation of the human ABC transporter ABCG2 on asparagine 596 is not essential for expression, transport activity, or trafficking to the plasma membrane. 6966283 0 37 gly alpha 47:51 arg1 the oligosaccharide chains alpha 1-protease inhibitor the oligosaccharide chains PUBTATOR alpha 1-protease inhibitor 5265 Studies on the oligosaccharide chains of human alpha 1-protease inhibitor. 10930422 3 17 gly glycosylated 297:308 arg1 Reprimo Reprimo PUBTATOR Reprimo 56475 Reprimo is a highly glycosylated protein and, when ectopically expressed, it is localized in the cytoplasm and induces G(2) arrest of the cell cycle. 18586680 4 45 part_of Asp-413 773:779 arg1 human O-GlcNAcase O-GlcNAcase Asp-413 PUBTATOR SpecificSite O-GlcNAcase 10724 Asp-413 The caspase-3 cleavage site of O-GlcNAcase, mapped by Edman sequencing, is a noncanonical recognition site that occurs after Asp-413 of the SVVD sequence in human O-GlcNAcase. 10978165 10 52 gly glycosylated 1253:1264 arg1 1-74 1-74 Cterm 1-74 6358 Our data imply that HCC-1 (1-74), HCC-1 (3-74), HCC-1 (4-74) and glycosylated HCC-1 (1-74) circulate in human blood. 10978165 10 52 gly glycosylated 1253:1264 arg1 glycosylated HCC-1 glycosylated HCC-1 PUBTATOR HCC-1 6358 Our data imply that HCC-1 (1-74), HCC-1 (3-74), HCC-1 (4-74) and glycosylated HCC-1 (1-74) circulate in human blood. 11439087 11 8 gly N-glycosylation 1428:1442 arg1 sBST-1 sBST-1 Cterm sBST-1 683 We conclude that N-glycosylation of sBST-1 facilitates the folding of the nascent polypeptide chain into a conformation that is conductive for intracellular transport and enzymic activity. 27384988 11 1 part_of rpS3 1244:1247 arg1 the Asn 165 residue rpS3 the Asn 165 residue PUBTATOR SpecificSite rpS3 6188 Asn 165 residue The results indicate that the Asn 165 residue of rpS3 is a critical site for N-linked glycosylation and passage through the ER-Golgi secretion pathway. 14573609 3 21 gly glycosylation 483:495 arg1 heparanase heparanase PUBTATOR heparanase 10855 In this study, we examined the link between glycosylation and the function of heparanase in human tumor cell lines. 7679920 8 52 gly AFP 1730:1732 arg1 the carbohydrate chains AFP the carbohydrate chains PUBTATOR AFP 174 These results indicate that the increment in fucosylation and branching to form new antennae is a characteristic feature of the carbohydrate chains of AFP from patients with neoplastic diseases of the liver. 24125761 6 81 gly glycosylated 878:889 arg1 AADAC AADAC PUBTATOR AADAC 13 This result indicated that AADAC was glycosylated at both N78 and N282. 18467335 12 69 gly PCI 1565:1567 arg1 the N-linked glycans PCI the N-linked glycans OGER PCI P05154 These results thus demonstrate that the N-linked glycans and the N-terminal region of blood-derived PCI in different ways affect the cofactor-enhanced rates of thrombin inhibition and provide information on the mechanisms by which this may be achieved. 2775174 7 13 gly contained 855:863 arg1 The 20 kDa subunit AND one high-mannose-type oligosaccharide chain The 20 kDa subunit one high-mannose-type oligosaccharide chain OGER subunit P20933 The 20 kDa subunit contained one high-mannose-type oligosaccharide chain, and the 24 kDa subunit had one high-mannose-type and one complex-type oligosaccharide chain. 2775174 7 1 gly had 933:935 arg1 the 24 kDa subunit AND complex-type the 24 kDa subunit complex-type OGER subunit P20933 The 20 kDa subunit contained one high-mannose-type oligosaccharide chain, and the 24 kDa subunit had one high-mannose-type and one complex-type oligosaccharide chain. 2775174 7 1 gly had 933:935 arg1 the 24 kDa subunit AND high-mannose-type the 24 kDa subunit high-mannose-type OGER subunit P20933 The 20 kDa subunit contained one high-mannose-type oligosaccharide chain, and the 24 kDa subunit had one high-mannose-type and one complex-type oligosaccharide chain. 8702840 6 7 gly modified 704:711 arg3 NF-H AND O-GlcNAc NF-H O-GlcNAc PUBTATOR NF-H 4744 Here we further report that NF-H is extensively modified by O-GlcNAc at Thr53, Ser54, and Ser56 in the head domain and, somewhat surprisingly, at multiple sites within the Lys-Ser-Pro repeat motif in the tail domain, a region in assembled neurofilaments known to be nearly stoichiometrically phosphorylated on each of the approximately 50 KSP repeats. 26536155 6 38 gly glycosylation 1501:1513 arg1 human IgG3 human IgG3 PUBTATOR IgG3 P01860 The approach was evaluated on glycoprotein standards and also applied to investigate the glycosylation of human IgG3 providing details on the hitherto uncharacterized glycosylation site Asn392 of the CH3 domain. 16407218 5 66 gly MBL 1009:1011 arg1 carbohydrate recognition domains MBL carbohydrate recognition domains PUBTATOR MBL 4153 We investigated the mechanism of formation of complexes between alpha2M and MBL and concluded that they form by the direct binding of oligomannose glycans Man(5-7) occupying Asn-846 on alpha2M to the lectin domains (carbohydrate recognition domains) of MBL. 24125761 12 36 gly N-glycosylation 1724:1738 arg1 AADAC AADAC PUBTATOR AADAC 13 Overall, this study found that the translational, but not post-translational, N-glycosylation of AADAC plays a crucial role in regulating AADAC enzyme activity. 18214858 6 42 gly derived 1085:1091 arg2 serum haptoglobin AND fucosylated N-glycans serum haptoglobin fucosylated N-glycans PUBTATOR haptoglobin 3240 While fucosylated N-glycans derived from serum haptoglobin of patients with CP slightly increased, di-fucosylated tetra-antennary N-glycans were observed only at this site in PC patients, and were absent in the haptoglobin of normal controls and individuals with CP. 21327254 5 5 gly sites 884:888 arg1 Tau Tau sites Cterm Tau Moreover, analytical difficulties have hampered the precise localization of the O-GlcNAc sites on Tau, except for the S400 site that was very recently identified on the basis of ETD-FT-MS. 22750213 5 17 part_of PRiMA 859:863 arg1 the asparagine-43 PRiMA the asparagine-43 PUBTATOR SpecificSite PRiMA 170952 asparagine-43 By using site-directed mutagenesis, the asparagine-43 was identified to be the N-linked glycosylation site of PRiMA. 14970177 3 48 part_of vIL-6 606:610 arg1 site N89 vIL site N89 OGER SpecificSite vIL P09327 N89 Here we show that vIL-6 is N-linked glycosylated at N78 and N89 and demonstrate that N-linked glycosylation at site N89 of vIL-6 markedly enhances binding to gp130, signaling through the JAK1-STAT1/3 pathway and functions in a cytokine-dependent cell proliferation bioassay. 24141704 0 48 gly glycosylation 38:50 arg1 Rho Rho OGER Rho P08100 A bacterial toxin catalyzing tyrosine glycosylation of Rho and deamidation of Gq and Gi proteins. 15628971 2 47 gly glycoprotein 335:346 arg1 purified C4ST-1 purified C4ST-1 PUBTATOR C4ST-1 314694 We showed previously that purified C4ST-1 from the culture medium of rat chondrosarcoma cells was a glycoprotein containing approx. 23431362 3 40 gly glycan 441:446 arg1 HIV-1 gp120 HIV-1 gp120 glycan PUBTATOR HIV-1 gp120 155971 One of these regions (N332) is characterized by an N-linked glycan at residue 332 on HIV-1 gp120 and is recognized by antibody 2G12 and by the recently reported antibodies PGT121-137, the latter isolated from three donors. 12421832 12 7 gly subunit 1655:1661 arg1 N-linked carbohydrate substitution PAPP-A subunit N-linked carbohydrate substitution PUBTATOR PAPP-A subunit 5069 We also show that of 13 potential sites for N-linked carbohydrate substitution of the PAPP-A subunit, 11 are occupied. 9574531 1 15 part_of DRA 171:173 arg1 Pro96-->Ser DRA Pro96-->Ser PUBTATOR AminoAcid DRA 1811 Pro96 The HLA-DR hemizygous B lymphoblastoid cell line, 10.24.6, has a DRA mutation (Pro96-->Ser) that creates a novel glycosylation site at Asn94. 468798 0 0 gly gonadotropin 81:92 arg1 the O-glycosidic carbohydrate units chorionic gonadotropin the O-glycosidic carbohydrate units OGER chorionic gonadotropin Structure and location of the O-glycosidic carbohydrate units of human chorionic gonadotropin. 9030779 0 59 gly N-glycosylation 35:49 arg1 human acid sphingomyelinase human acid sphingomyelinase PUBTATOR acid sphingomyelinase 6609 Functional characterization of the N-glycosylation sites of human acid sphingomyelinase by site-directed mutagenesis. 2110822 0 58 gly protein 76:82 arg1 asparagine-linked sugar chains activator protein 1 asparagine-linked sugar chains PUBTATOR activator protein 1 5660 Characteristics of asparagine-linked sugar chains of sphingolipid activator protein 1 purified from normal human liver and GM1 gangliosidosis (type 1) liver. 20427278 0 75 gly glycosylation 52:64 arg1 the amyloid precursor protein the amyloid precursor protein OGER amyloid precursor protein P05067 The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. 1457969 5 43 gly present 877:883 arg1 hTSH AND The N-glycans hTSH The N-glycans OGER hTSH The N-glycans present on hTSH were mainly diantennary complex-type structures with a common Man alpha 1-3 branch that terminated with 4-O-sulphated GalNAc. 1457969 5 43 gly present 877:883 arg2 hTSH AND diantennary complex-type structures hTSH diantennary complex-type structures OGER hTSH The N-glycans present on hTSH were mainly diantennary complex-type structures with a common Man alpha 1-3 branch that terminated with 4-O-sulphated GalNAc. 14749323 8 5 gly glycosylation 1484:1496 arg1 OAT function OAT function OGER OAT P04181 Single replacement of asparagines at other sites had no effect on transport activity indicating that glycosylation at individual sites is not essential for OAT function. 15807535 7 77 gly glycosylated 1346:1357 arg1 the glycosylated ABCG2 protein the glycosylated ABCG2 protein PUBTATOR ABCG2 protein Q9UNQ0 Cell surface analysis of ABCG2 expression showed comparable amounts of the N596Q variant present at the plasma membrane compared to the glycosylated ABCG2 protein. 19022411 8 49 part_of GAPDH 1362:1366 arg1 Thr227 GAPDH Thr227 PUBTATOR AminoAcid GAPDH 2597 Thr227 The present study identified Thr227 as the major GAPDH O-GlcNAcylation site, which suggests that this modification mediates the nuclear translocation of GAPDH, presumably by disrupting the conformation of tetrameric GAPDH. 16763549 3 40 gly NRP1 501:504 arg1 proteoglycan NRP1 proteoglycan OGER NRP1 O14786 Here we show that a substantial fraction of NRP1 is proteoglycan modified with either heparan sulfate or chondroitin sulfate on a single conserved Ser. 2511201 11 58 gly structure 1868:1876 arg1 human factors VII VII structure Cterm VII In relation to the trisaccharide sugar chain previously discovered in bovine factors VII and IX, these findings indicate the existence of a Xyl2-Glc-Ser and a Xyl-Glc-Ser structure in the first epidermal growth factor-like domains of human factors VII and IX and protein Z in addition to that of bovine protein Z. Whether these carbohydrate moieties contribute to the biological activities of these proteins is unknown. 2511201 11 58 gly structure 1868:1876 arg1 protein Z protein Z structure Cterm protein Z In relation to the trisaccharide sugar chain previously discovered in bovine factors VII and IX, these findings indicate the existence of a Xyl2-Glc-Ser and a Xyl-Glc-Ser structure in the first epidermal growth factor-like domains of human factors VII and IX and protein Z in addition to that of bovine protein Z. Whether these carbohydrate moieties contribute to the biological activities of these proteins is unknown. 23187000 5 44 gly glycosylation 880:892 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 Because the TIMP-1 glycosylation participate in the interaction, aberrant glycosylation of TIMP-1 presumably affects the interaction, thereby leading to pathogenic dysfunction in cancer cells. 17980170 11 51 gly N-glycosylation 1281:1295 arg1 human NAAA human NAAA PUBTATOR NAAA 27163 We next determined N-glycosylation sites of human NAAA by site-directed mutagenesis addressed to asparagine residues in six potential N-glycosylation sites. 7620335 1 59 gly has 159:161 arg1 Human blood coagulation factor X AND two N-linked oligosaccharides Human blood coagulation factor X two N-linked oligosaccharides OGER factor X P00742 Human blood coagulation factor X has two N-linked oligosaccharides at Asn39 and Asn49 residues and two O-linked oligosaccharides at Thr17 and Thr29 residues in the region of the factor X activation peptide (XAP) which is cleaved off during its activation by factor IXa. 7620335 1 59 gly has 159:161 arg1 Human blood coagulation factor X AND two O-linked oligosaccharides Human blood coagulation factor X two O-linked oligosaccharides OGER factor X P00742 Human blood coagulation factor X has two N-linked oligosaccharides at Asn39 and Asn49 residues and two O-linked oligosaccharides at Thr17 and Thr29 residues in the region of the factor X activation peptide (XAP) which is cleaved off during its activation by factor IXa. 8216207 0 17 gly site 63:66 arg1 soluble recombinant human thrombomodulin thrombomodulin site PUBTATOR thrombomodulin 7056 Identification of the predominant glycosaminoglycan-attachment site in soluble recombinant human thrombomodulin: potential regulation of functionality by glycosyltransferase competition for serine474. 23319596 10 10 gly hLOXL2 1578:1583 arg1 the N-glycan at Asn-644 hLOXL2 the N-glycan at Asn-644 PUBTATOR hLOXL2 4017 These results suggest that the N-glycan at Asn-644 of hLOXL2 enhances the solubility and stability of the LOX catalytic domain. 6966283 3 52 gly has 143:145 arg1 Human alpha 1-protease inhibitor AND three oligosaccharide side chains Human alpha 1-protease inhibitor three oligosaccharide side chains PUBTATOR Human alpha 1-protease inhibitor 5265 Human alpha 1-protease inhibitor has three oligosaccharide side chains attached to 3 separate asparaginyl residues of the protein by N-glycosyl linkages. 22688517 3 77 gly N-glycosylation 362:376 arg1 LOX-1 LOX-1 PUBTATOR LOX-1 4973 However, the N-glycosylation pattern of LOX-1 has not been described yet. 2108149 5 12 gly possessed 848:856 arg1 IgGs AND oligosaccharide units IgGs oligosaccharide units Cterm IgGs IgGs from both parental lines possessed oligosaccharide units displaying microheterogeneity based upon a common symmetrical biantennary structure terminating in beta-GlcNAc. 12151713 5 20 gly N-glycosylation 629:643 arg1 recombinant lactoferrin recombinant lactoferrin OGER lactoferrin P02788 The results indicated that both N-glycosylation sites of recombinant lactoferrin are mainly substituted by typical plant paucimannose-type glycans, with beta1,2-xylose and alpha1,3-linked fucose at the proximal N-acetylglucosamine, and that complex-type glycans with Lewis(a) determinants are not present in maize recombinant lactoferrin. 12151713 5 21 gly present 894:900 arg1 maize recombinant lactoferrin AND complex-type glycans lactoferrin complex-type glycans OGER lactoferrin P02788 The results indicated that both N-glycosylation sites of recombinant lactoferrin are mainly substituted by typical plant paucimannose-type glycans, with beta1,2-xylose and alpha1,3-linked fucose at the proximal N-acetylglucosamine, and that complex-type glycans with Lewis(a) determinants are not present in maize recombinant lactoferrin. 7780197 0 45 gly glycosylation 13:25 arg1 human transferrin receptor human transferrin receptor PUBTATOR transferrin receptor 7037 The critical glycosylation site of human transferrin receptor contains a high-mannose oligosaccharide. 7780197 0 65 gly contains 62:69 arg1 human transferrin receptor AND a high-mannose oligosaccharide human transferrin receptor The critical glycosylation site a high-mannose oligosaccharide PUBTATOR transferrin receptor 7037 site The critical glycosylation site of human transferrin receptor contains a high-mannose oligosaccharide. 20406422 3 38 gly non-glycosylated 487:502 arg1 Both partially and non-glycosylated CLN7 Both partially and non-glycosylated CLN7 PUBTATOR CLN7 256471 Both partially and non-glycosylated CLN7 were correctly transported to lysosomes. 20595044 1 21 gly glycoprotein 92:103 arg1 MD-1 MD-1 OGER MD-1 O88188 MD-1 is a glycoprotein that associates with a B-cell-specific RP105 protein and has a low sequence identity of 16% to MD-2 that associates with Toll-like receptor 4 and recognizes endotoxic lipopolysaccharide. 12970363 5 58 gly glycosylation 842:854 arg1 TRPC6 TRPC6 PUBTATOR TRPC6 7225 To identify potential molecular correlates accounting for the functional difference, we analyzed the glycosylation pattern of TRPC6 compared with TRPC3. 2415652 8 5 gly desialylated 1487:1498 arg1 desialylated JAr hCG beta desialylated JAr hCG beta PUBTATOR JAr hCG beta 1082 Like desialylated ElBre hCG beta, desialylated JAr hCG beta bound completely to peanut agglutinin, but was incompletely recognized by antisera to the hCG beta-CTE. 2415652 8 62 gly desialylated 1458:1469 arg1 desialylated ElBre hCG beta desialylated ElBre hCG beta PUBTATOR ElBre hCG beta 1082 Like desialylated ElBre hCG beta, desialylated JAr hCG beta bound completely to peanut agglutinin, but was incompletely recognized by antisera to the hCG beta-CTE. 20511397 5 58 part_of Thr 1037:1039 arg1 both cellular and secreted apoE apoE Thr PUBTATOR SpecificSite apoE 348 Thr(194) Our results identify eight different glycoforms with (HexNAc)(2)-Hex(2)-(NeuAc)(2) being the most complex glycan detected on Thr(194) in both cellular and secreted apoE. 15054092 4 55 gly glycosylated 624:635 arg1 G5 G5 Cterm G5 Q9H222 Site-directed mutagenesis revealed that two asparagine residues (Asn(585) and Asn(592)) are glycosylated in G5 and that G8 has a single N-linked glycan attached to Asn(619). 15054092 4 33 gly has 655:657 arg1 G8 AND a single N-linked glycan G8 a single N-linked glycan Cterm G8 Q9H221 Site-directed mutagenesis revealed that two asparagine residues (Asn(585) and Asn(592)) are glycosylated in G5 and that G8 has a single N-linked glycan attached to Asn(619). 28327546 3 81 gly N-glycosylation 340:354 arg1 FVIII FVIII PUBTATOR FVIII 2157 Here we characterize how hemophilia mutations near the unused N-glycosylation site of the A2 domain (N582) of FVIII affect protein conformation and intracellular trafficking. 23167757 5 56 gly glycosylation 875:887 arg1 human PDIA2 human PDIA2 PUBTATOR PDIA2 64714 By site-directed mutagenesis and enzymatic deglycosylation, we show here that all three Asn residues within the potential N-linked glycosylation sites of human PDIA2 (N127, N284 and N516) are glycosylated in human cells. 25485983 0 10 part_of antithrombin 84:95 arg1 N135 antithrombin N135 PUBTATOR SpecificSite antithrombin 462 N135 Increased N-glycosylation efficiency by generation of an aromatic sequon on N135 of antithrombin. 6689265 2 34 gly glycosylated 362:373 arg1 Gc 1 protein Gc 1 protein PUBTATOR Gc 1 protein 10562 It was found that only the Gc 1 protein (Gc1a isoform) was glycosylated, the glycan moiety representing about 1% of the protein. 3422739 4 43 gly have 593:596 arg1 Ii AND multiple candidate glycosaminoglycan-attachment sites Ii multiple candidate glycosaminoglycan-attachment sites Cterm Ii Ii does not have multiple candidate glycosaminoglycan-attachment sites, and we used site-directed mutagenesis to replace a candidate serine glycosaminoglycan-acceptor site with alanine at position 201 in the murine Ii protein. 18227435 5 36 gly O-glycosylated 873:886 arg1 (3) P17 (3) P17 PUBTATOR 3) P17 653820 Our findings are as follows: (1) the N-terminal amino acid of P16 and P17 is pyroglutamic acid; (2) the Ser residue at the sixth position of P16 is phosphorylated; (3) P17 is O-glycosylated at Thr10; and (4) the C-terminal amino acid of P16 and P17 is truncated. 8636209 8 53 gly glycosylation 1015:1027 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 In contrast, treatment of T cells with tunicamycin suggested that N-linked glycosylation of CD3 delta is required for TCR assembly. 8636209 8 53 gly glycosylation 1015:1027 arg1 CD3 delta CD3 delta PUBTATOR CD3 delta 915 In contrast, treatment of T cells with tunicamycin suggested that N-linked glycosylation of CD3 delta is required for TCR assembly. 18775496 3 2 gly Fucosylation 408:419 arg1 recombinant C45 agrin recombinant C45 agrin PUBTATOR C45 agrin O00468 Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). 18775496 3 40 gly agrin 440:444 arg1 Fucosylation C45 agrin Fucosylation PUBTATOR C45 agrin O00468 Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). 24161696 8 61 gly N-glycosylation 1492:1506 arg1 Kv3.1b Kv3.1b Cterm Kv3.1b CONCLUSIONS: Our findings provide direct evidence that occupancy of the N-glycosylation sites of Kv3.1b contributes significantly to its lateral heterogeneity in membranes of neuronal-derived cells, and in turn alters cellular properties. 9712881 0 70 part_of protein 55:61 arg1 Glu681 AE1 protein Glu681 PUBTATOR AminoAcid AE1 protein 6521 Glu681 Topology of the region surrounding Glu681 of human AE1 protein, the erythrocyte anion exchanger. 25265424 3 25 gly glycoforms 356:365 arg1 hemopexin hemopexin PUBTATOR hemopexin 3263 We present a case study that compares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. 25265424 3 25 gly glycoforms 356:365 arg1 kininogen-1 kininogen-1 PUBTATOR kininogen-1 3827 We present a case study that compares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. 25265424 3 25 gly glycoforms 356:365 arg1 complement factor H haptoglobin, complement factor H PUBTATOR haptoglobin, complement factor H 3240 We present a case study that compares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. 25374123 3 56 gly N-glycopeptides 492:506 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 Here, we systematically analyzed the site-specific N-glycopeptides of vitronectin in human plasma by tandem mass spectrometry combined with immunoprecipitation and hydrophilic interaction liquid chromatography (HILIC) enrichment. 15454184 2 66 gly glycosylation 308:320 arg1 recombinant human FS (rhFS) recombinant human FS (rhFS) Cterm rhFS In this study, we analyzed the glycosylation of recombinant human FS (rhFS) produced in Chinese hamster ovary cells. 10988252 9 12 gly glycosylation 1604:1616 arg1 sEGFR sEGFR PUBTATOR EGFR 1956 This first comprehensive glycosylation study on a human nonrecombinant receptor shows the immense heterogeneity of the glycosylation of sEGFR. 2013294 1 53 gly glycoprotein 118:129 arg1 Secretory actin-binding protein Secretory actin-binding protein PUBTATOR Secretory actin-binding protein 5304 Secretory actin-binding protein (SABP), a glycoprotein from human seminal plasma, was isolated according to Akiyama and Kimura [Akiyama, K. & Kimura, H. (1990) Biochim. 8670172 5 88 part_of Asn-77 888:893 arg1 a GPI-anchor GPI Asn-77 OGER SpecificSite GPI P06744 Asn-77 Mass analysis of the isolated C-terminal peptide (T9) indicated that a GPI-anchor (at Asn-77) without an inositol-associated phospholipid was present in soluble CD59u. 10814696 3 68 gly A 786:786 arg1 carbohydrate moieties human placental arylsylfatase A carbohydrate moieties Cterm human placental arylsylfatase A In the present study we have analyzed carbohydrate moieties of human placental arylsylfatase A using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting on Immobilon P and on-blot deglycosylation using PNGase F for glycan release. 6118137 0 53 gly glycoprotein 16:27 arg1 Rat brain Thy-1 glycoprotein Rat brain Thy-1 glycoprotein PUBTATOR Thy-1 glycoprotein 24832 Rat brain Thy-1 glycoprotein. 9169007 7 5 part_of antithrombin 1124:1135 arg1 Asn 155 antithrombin Asn 155 PUBTATOR SpecificSite antithrombin 462 Asn 155 These results demonstrate that heterogeneous glycosylation of Asn 155 of recombinant antithrombin is responsible for generating the low heparin affinity glycoform. 11279095 2 1 gly Polysialylation 190:204 arg1 NCAM NCAM PUBTATOR NCAM 4684 Polysialylation of the neural cell adhesion molecule (NCAM) is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which contain sialylmotifs L and S conserved in all members of the sialyltransferases. 11279095 2 1 gly Polysialylation 190:204 arg1 the neural cell adhesion molecule the neural cell adhesion molecule PUBTATOR neural cell adhesion molecule 4684 Polysialylation of the neural cell adhesion molecule (NCAM) is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which contain sialylmotifs L and S conserved in all members of the sialyltransferases. 11279095 2 52 gly molecule 234:241 arg1 Polysialylation neural cell adhesion molecule Polysialylation PUBTATOR neural cell adhesion molecule 4684 Polysialylation of the neural cell adhesion molecule (NCAM) is catalyzed by two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), which contain sialylmotifs L and S conserved in all members of the sialyltransferases. 8286855 9 49 gly glycosylation 1234:1246 arg1 GpA GpA PUBTATOR GpA 2993 These simple rules explain the glycosylation (or lack of it) on 21 of 22 Ser/Thr in the extracellular domain of GpA. 23661698 4 48 gly deglycosylated 518:531 arg1 Ephrin-A1 Ephrin-A1 PUBTATOR Ephrin-A1 1942 Ephrin-A1 was enzymatically deglycosylated, and its activity was evaluated in several assays using glioblastoma (GBM) cells and recombinant EphA2. 22750213 0 45 gly glycosylation 9:21 arg1 globular tetrameric acetylcholinesterase globular tetrameric acetylcholinesterase PUBTATOR acetylcholinesterase 11423 N-linked glycosylation of proline-rich membrane anchor (PRiMA) is not required for assembly and trafficking of globular tetrameric acetylcholinesterase. 22750213 0 45 gly glycosylation 9:21 arg1 PRiMA PRiMA PUBTATOR PRiMA 170952 N-linked glycosylation of proline-rich membrane anchor (PRiMA) is not required for assembly and trafficking of globular tetrameric acetylcholinesterase. 8702538 10 29 gly glycoproteins 1714:1726 arg1 the CD22 and CD33 glycoproteins the CD22 and CD33 glycoproteins PUBTATOR CD33 glycoproteins 945 These observations suggest that a single N-linked glycosylation site located at a similar position in the CD22 and CD33 glycoproteins is critical for regulating ligand recognition by both receptors. 11805077 0 13 gly oligosaccharides 41:56 arg1 erythropoietin erythropoietin oligosaccharides OGER erythropoietin P01588 Structural analysis of sulfated N-linked oligosaccharides in erythropoietin. 19855092 10 88 gly desialylated 1724:1735 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin Q15848 Finally, after chronic administration in adiponectin(-/-) mice steady-state levels of desialylated adiponectin were lower than control adiponectin and failed to recapitulate the improvements in glucose and insulin tolerance tests observed with control adiponectin. 10092871 7 73 gly carry 1135:1139 arg1 MMP-1 AND alpha 2,3-sialylated complex-type diantennary glycans MMP-1 alpha 2,3-sialylated complex-type diantennary glycans PUBTATOR MMP-1 4312 MMP-1 derived from fibroblasts was found to carry mainly alpha 2,3-sialylated complex-type diantennary glycans. 25568315 9 17 gly N-glycosylation 1681:1695 arg1 the membrane processed NBCe1-A dimer the membrane processed NBCe1-A dimer PUBTATOR NBCe1-A dimer 8671 Moreover, the formation of this unique structure is critically dependent on the finely tuned interplay between disulfide bonding and N-glycosylation in the membrane processed NBCe1-A dimer. 9933650 6 49 part_of IL-1-dependent 910:923 arg1 D10-G4 IL-1 D10-G4 PUBTATOR SiteSequence IL-1 3552 D10-G4 The oligosaccharides in fraction AR also inhibited the growth of an IL-1-dependent cell line, D10-G4. 17542669 5 27 part_of 2,500 690:694 arg1 2,500 A large (2,500 A(2) OGER SpecificSite large (2,500 Q8N3Y3 A(2) The interface buried in the complex is large (2,500 A(2)) and an extensive network of ionic, polar, and hydrophobic bonding is involved in the interaction. 21752865 8 19 gly N-glycosylation 1296:1310 arg1 BRI2 trafficking BRI2 trafficking PUBTATOR BRI2 9445 To examine the effect of N-glycosylation on BRI2 trafficking at the cell surface, we performed biotinylation and (35)S methionine pulse-chase experiments. 27314333 10 39 gly Rspo1 1590:1594 arg1 N-glycan Rspo1 N-glycan PUBTATOR Rspo1 284654 While N-glycan of Rspo1 plays a role in its intracellular stability, it had little effect on secreted Rspo1. 1731338 6 60 gly galactoglycoprotein 1001:1019 arg1 the galactoglycoprotein polypeptide the galactoglycoprotein polypeptide PUBTATOR galactoglycoprotein 6693 A search of a protein data base revealed that the galactoglycoprotein polypeptide is identical to the N-terminal (extracellular) polypeptide region of the blood-cell surface molecule CD43 (sialophorin, leukosialin). 7068558 0 44 gly lactoferrin 46:56 arg1 the sugar chains lactoferrin the sugar chains OGER lactoferrin P02788 Structural study of the sugar chains of human lactoferrin: finding of four novel complex-type asparagine-linked sugar chains. 6192908 1 2 gly alpha-fetoprotein 94:110 arg1 The carbohydrate moiety alpha-fetoprotein The carbohydrate moiety PUBTATOR alpha-fetoprotein 174 The carbohydrate moiety of alpha-fetoprotein purified from human yolk sac tumors grown in nude mice was quantitatively released from the polypeptide chain as oligosaccharides by hydrazinolysis. 22750213 3 41 gly glycosylation 492:504 arg1 T T Cterm T 11423 The glycosylation of AChE(T) is known to be required for its proper assembly and trafficking; however, the role of PRiMA glycosylation in the oligomer assembly has not been revealed. 22750213 3 41 gly glycosylation 492:504 arg1 AChE AChE PUBTATOR AChE 11423 The glycosylation of AChE(T) is known to be required for its proper assembly and trafficking; however, the role of PRiMA glycosylation in the oligomer assembly has not been revealed. 20739279 5 10 gly N-glycan 742:749 arg1 residue residue residue SpecificSite residue Asn(60) Through crystallographic analysis of SynCAM 2, we identified within the adhesive interface of its Ig1 domain an N-glycan on residue Asn(60). 1731338 7 36 gly galactoglycoprotein 1273:1291 arg1 125I-labeled galactoglycoprotein 125I-labeled galactoglycoprotein PUBTATOR 125I-labeled galactoglycoprotein 6693 Further support of the relatedness of these molecules was obtained by immunoprecipitation of 125I-labeled galactoglycoprotein by monoclonal anti-CD43 antibodies. 30127001 9 46 gly site 1495:1498 arg1 serine 435 serine 435 serine 435 SpecificSite serine 435 Mutation of the O-Glc modification site on EGF11 (serine 435) in combination with sensitizing O-fucose mutations in EGF8 or EGF12 affected cell-surface presentation of NOTCH1 or reduced activation of NOTCH1 by Delta-like1, respectively. 24134926 9 64 part_of HAS2 1646:1649 arg1 serine 221 residue HAS2 serine 221 residue PUBTATOR SpecificSite HAS2 3037 serine 221 residue Such highly dynamic and ubiquitous protein modification affects serine 221 residue of HAS2 that lead to a dramatic stabilization of the enzyme in the membranes. 22678432 7 8 gly IgG 1212:1214 arg1 released glycans IgG released glycans Cterm IgG The data obtained from analyzing released glycans of rHuEPO and IgG, described in the second protocol of this series (10.1038/nprot.2012.063), provide complementary detailed glycan structural information that facilitates characterization of the glycopeptides. 22678432 7 14 gly rHuEPO 1201:1206 arg1 released glycans rHuEPO released glycans Cterm rHuEPO 2056 The data obtained from analyzing released glycans of rHuEPO and IgG, described in the second protocol of this series (10.1038/nprot.2012.063), provide complementary detailed glycan structural information that facilitates characterization of the glycopeptides. 7451505 2 13 gly released 220:227 arg2 human ceruloplasmin AND the oligosaccharides human ceruloplasmin the oligosaccharides PUBTATOR ceruloplasmin 1356 The structures of the oligosaccharides released from human ceruloplasmin by hydrazinolysis were studied by sequential exoglycosidase digestion in combination with methylation analysis and periodate oxidation. 1371281 9 14 gly glycosylated 1231:1242 arg1 CK8 CK8 PUBTATOR CK8 3856 Our results show that CK8 and 18 are glycosylated at multiple sites with a single O-linked N-acetylglucosamine. 8390218 0 85 gly 1-antitrypsin 55:67 arg1 the sugar chains alpha 1-antitrypsin the sugar chains PUBTATOR alpha 1-antitrypsin 5265 Structural analysis on the sugar chains of human alpha 1-antitrypsin: presence of fucosylated biantennary glycan in hepatocellular carcinoma. 25517345 3 6 gly deletions 457:465 arg1 gp120 gp120 deletions PUBTATOR gp120 155971 It has been shown that long-term CBA pressure in vitro can result in mutant HIV-1 isolates with several N-linked glycan deletions on gp120. 6689265 1 28 gly protein 198:204 arg1 the carbohydrate chain vitamin D-binding protein the carbohydrate chain PUBTATOR vitamin D-binding protein 2638 On a highly purified preparation, the structure of the carbohydrate chain of the human vitamin D-binding protein was investigated and two genetic forms of this protein were considered (Gc 2 and Gc 1 proteins). 23661698 9 52 gly glycosylation 1255:1267 arg1 ephrin-A1 ephrin-A1 PUBTATOR ephrin-A1 1942 These findings suggest that the glycosylation on ephrin-A1 plays a critical role in the binding and activation of the EphA2 receptor. 3422739 1 46 gly has 296:298 arg1 Ii AND a proteoglycan form Ii a proteoglycan form Cterm (Ii) The invariant chain (Ii), a nonpolymorphic glycoprotein that associates with the immunoregulatory Ia proteins encoded by the major histocompatibility complex, has a proteoglycan form (Ii-CS) that bears a chondroitin sulfate glycosaminoglycan. 3422739 1 18 gly bears 333:337 arg1 Ii-CS AND a chondroitin sulfate glycosaminoglycan Ii-CS a chondroitin sulfate glycosaminoglycan Cterm Ii The invariant chain (Ii), a nonpolymorphic glycoprotein that associates with the immunoregulatory Ia proteins encoded by the major histocompatibility complex, has a proteoglycan form (Ii-CS) that bears a chondroitin sulfate glycosaminoglycan. 28207759 3 67 gly containing 437:446 arg1 recombinant GBA AND mannose-terminated N-linked glycans recombinant GBA mannose-terminated N-linked glycans PUBTATOR GBA 2629 Supplementing storage cells with lacking enzyme is accomplished via chronic intravenous administration of recombinant GBA containing mannose-terminated N-linked glycans, mediating the selective uptake by macrophages expressing mannose-binding lectin(s). 16964247 2 19 part_of p53 294:296 arg1 Ser 149 p53 Ser 149 PUBTATOR SpecificSite p53 7157 Ser 149 Here, we show that Ser 149 of p53 is O-GlcNAcylated and that this modification is associated with decreased phosphorylation of p53 at Thr 155, which is a site that is targeted by the COP9 signalosome, resulting in decreased p53 ubiquitination. 7541354 7 36 gly oligosaccharides 1175:1190 arg1 human vitronectin vitronectin oligosaccharides PUBTATOR vitronectin 7448 The possibility that several binding activities of vitronectin can be ascribed to its glycan moiety was discussed, based on the specific features of the N-linked oligosaccharides on human vitronectin revealed here. 10978165 9 13 gly nonglycosylated 1128:1142 arg1 1-74 1-74 Cterm 1-74 6358 In hemofiltrate approximately 3% of total HCC-1 represents HCC-1 (3-74) and approximately 1% represents HCC-1 (4-74) whereas the major products are nonglycosylated HCC-1 (1-74) and glycosylated HCC-1 (1-74). 10978165 9 13 gly nonglycosylated 1128:1142 arg1 nonglycosylated HCC-1 nonglycosylated HCC-1 PUBTATOR HCC-1 6358 In hemofiltrate approximately 3% of total HCC-1 represents HCC-1 (3-74) and approximately 1% represents HCC-1 (4-74) whereas the major products are nonglycosylated HCC-1 (1-74) and glycosylated HCC-1 (1-74). 10978165 9 18 gly glycosylated 1161:1172 arg1 1-74 1-74 Cterm 1-74 6358 In hemofiltrate approximately 3% of total HCC-1 represents HCC-1 (3-74) and approximately 1% represents HCC-1 (4-74) whereas the major products are nonglycosylated HCC-1 (1-74) and glycosylated HCC-1 (1-74). 10978165 9 18 gly glycosylated 1161:1172 arg1 glycosylated HCC-1 glycosylated HCC-1 PUBTATOR HCC-1 6358 In hemofiltrate approximately 3% of total HCC-1 represents HCC-1 (3-74) and approximately 1% represents HCC-1 (4-74) whereas the major products are nonglycosylated HCC-1 (1-74) and glycosylated HCC-1 (1-74). 10929010 2 31 gly glycoforms 398:407 arg1 multiple transferrin glycoforms multiple transferrin glycoforms PUBTATOR transferrin 7018 Metabolic radiolabeling of the intra- and extracellular protein fractions revealed the presence of multiple transferrin glycoforms with molecular weights lower than that observed for native human transferrin. 8323299 5 71 gly glycosylation 697:709 arg1 lamp-2 lamp-2 PUBTATOR In lamp-2 3920 In lamp-2, complete glycosylation was found at Ser-167, Thr-168, Thr-172, Thr-175, Thr-176, Thr-182, and Thr-183, and partial glycosylation at Ser-179 and Thr-181, and possibly also at Thr-185. 30127001 3 42 gly NOTCH1 497:502 arg1 Epidermal Growth Factor-like (EGF) repeat 11 NOTCH1 Epidermal Growth Factor-like (EGF) repeat 11 PUBTATOR NOTCH1 4851 This serine occurs between conserved cysteines 3 and 4 of Epidermal Growth Factor-like (EGF) repeat 11 of NOTCH1, a site distinct from those modified by protein O-glucosyltransferase 1 (POGLUT1), suggesting that a different enzyme is responsible. 23001782 1 72 gly glycoprotein 195:206 arg1 Human sex hormone binding globulin Human sex hormone binding globulin PUBTATOR Human sex hormone binding globulin 6462 Human sex hormone binding globulin (hSHBG) is a serum glycoprotein central to the transport and targeted delivery of sex hormones to steroid-sensitive tissues. 7541354 6 62 gly vitronectin 738:748 arg1 The major oligosaccharides vitronectin The major oligosaccharides PUBTATOR vitronectin 7448 The major oligosaccharides of human vitronectin were of the diantennary N-acetyllactosamine type, with a lesser amount of the tri- and a small amount of the mono-antennary type, to which 1-3 mol sialic acid residues were linked, mostly through alpha 2-6 linkages, although alpha 2-3 linkages were also present. 10386995 3 21 gly sites 619:623 arg1 synapsin I synapsin I sites PUBTATOR synapsin I 24949 Here, we identified seven in vivo O-GlcNAcylation sites on synapsin I by analysis of HPLC-purified digests of rat brain synapsin I. 8687384 1 14 gly glycosylation 191:203 arg1 t-PA t-PA PUBTATOR t-PA 5327 The glycosylation of tissue plasminogen activator (t-PA) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing. 8687384 1 14 gly glycosylation 191:203 arg1 tissue plasminogen activator tissue plasminogen activator OGER tissue plasminogen activator P00750 The glycosylation of tissue plasminogen activator (t-PA) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing. 10200178 10 79 gly epitopes 1845:1852 arg1 murine PrPSc PrPSc epitopes PUBTATOR PrPSc 19122 The abundance of the Lewisx and sialyl Lewisx epitopes on murine PrPSc may indicate a role for these structures in the normal function of PrPC or the pathophysiology of PrPSc. 1694784 1 40 gly isolated 205:212 arg2 the reduced and carboxymethylated human protein HC AND Three different carbohydrate prosthetic groups the reduced and carboxymethylated human protein HC Three different carbohydrate prosthetic groups PUBTATOR protein HC 259 Three different carbohydrate prosthetic groups associated to three chymotryptic peptides, Q1, Q2 and Q3, were isolated from the reduced and carboxymethylated human protein HC. 7727388 2 56 gly glycoprotein 242:253 arg1 a disulfide-linked homodimer a disulfide-linked homodimer PUBTATOR homodimer 6647 This cell surface glycoprotein is a disulfide-linked homodimer with a subunit molecular weight of 68,000. 3179269 2 41 gly released 408:415 arg1 each EPO protein AND Asparagine-linked neutral oligosaccharides each EPO protein Asparagine-linked neutral oligosaccharides PUBTATOR EPO protein 2056 Asparagine-linked neutral oligosaccharides were released from each EPO protein by N-oligosaccharide glycopeptidase (almond) digestion. 20489211 3 70 part_of fibronectin 629:639 arg1 D4-D6 fibronectin D4-D6 PUBTATOR SiteSequence fibronectin 2335 D4-D6 Here we report the crystal structure of the entire extracellular portion of human gp130 (domains 1-6, D1-D6) at 3.6 A resolution, in an unliganded form, as well as a higher resolution structure of the membrane-proximal fibronectin type III domains (D4-D6) at 1.9 A. 10764840 0 49 gly N-glycosylation 8:22 arg1 a recombinant human erythropoietin a recombinant human erythropoietin PUBTATOR erythropoietin 2056 Unusual N-glycosylation of a recombinant human erythropoietin expressed in a human lymphoblastoid cell line does not alter its biological properties. 1899031 0 20 part_of plasminogen 74:84 arg1 asparagine-289 plasminogen asparagine-289 PUBTATOR SpecificSite plasminogen 5340 asparagine-289 Oligosaccharide structures present on asparagine-289 of recombinant human plasminogen expressed in a Chinese hamster ovary cell line. 9334252 8 13 gly C-mannosylated 1457:1470 arg1 Recombinant RNase 2 Recombinant RNase 2 PUBTATOR Recombinant RNase 2 6036 Recombinant RNase 2 isolated from insect cells, plant protoplasts, and Escherichia coli was not C-mannosylated. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr6 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Ser2 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr10 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr15 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Ser2 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr10 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr15 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr10 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr15 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 21264968 4 127 part_of containing 1629:1638 arg1 densely glycosylated MUC4 AND Thr15 MUC4 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 PUBTATOR AminoAcid MUC4 4585 residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15 In the present study, O-glycosylation was initiated specifically at Thr10 in naked MUC4 peptide and additional introduction of α-GalNAc proceeded preferentially but randomly at three other Thr residues to afford densely glycosylated MUC4 containing six α-GalNAc residues at Thr1, Ser2, Ser5, Thr6, Thr10, and Thr15. 2415652 5 31 gly beta 1142:1145 arg1 the carbohydrate-sensitive antiserum R141 hCG beta the carbohydrate-sensitive antiserum R141 PUBTATOR hCG beta 1082 ElBre hCG beta, however, was incompletely recognized by antisera specific for the CTE of standard hCG beta, especially the carbohydrate-sensitive antiserum R141. 22601780 3 6 gly contains 344:351 arg1 Del-1 AND 3 epidermal growth factor (EGF)-like repeats Del-1 3 epidermal growth factor (EGF)-like repeats PUBTATOR Del-1 10085 Del-1 contains 3 epidermal growth factor (EGF)-like repeats and 2 discoidin-like domains. 12970363 7 86 gly monoglycosylated 1252:1267 arg1 the monoglycosylated TRPC3 channel the monoglycosylated TRPC3 channel PUBTATOR TRPC3 channel 7222 Immunoblotting analysis of HEK 293 cell lysates expressing TRPC6 wild type and mutants favors a model of TRPC6 that is dually glycosylated within the first (e1) and second extracellular loop (e2) as opposed to the monoglycosylated TRPC3 channel (Vannier, B., Zhu, X., Brown, D., and Birnbaumer, L. (1998) J. Biol. 6684483 1 32 gly contains 130:137 arg1 Sex hormone-binding globulin AND one O-linked oligosaccharide Sex hormone-binding globulin one O-linked oligosaccharide PUBTATOR Sex hormone-binding globulin 6462 Sex hormone-binding globulin from human blood serum contains two biantennary N-linked oligosaccharide chains of the N-acetyllactosamine type and one O-linked oligosaccharide per one molecule of the glycoprotein. 6684483 1 32 gly contains 130:137 arg1 Sex hormone-binding globulin AND two biantennary N-linked oligosaccharide chains Sex hormone-binding globulin two biantennary N-linked oligosaccharide chains PUBTATOR Sex hormone-binding globulin 6462 Sex hormone-binding globulin from human blood serum contains two biantennary N-linked oligosaccharide chains of the N-acetyllactosamine type and one O-linked oligosaccharide per one molecule of the glycoprotein. 7781780 0 29 gly erythropoietin 119:132 arg1 a mannose-6-phosphate containing oligomannosidic N-glycan erythropoietin a mannose-6-phosphate containing oligomannosidic N-glycan PUBTATOR erythropoietin 2056 Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoietin secreted by recombinant BHK-21 cells. 108268 1 40 gly chain 109:113 arg1 the oligosaccharide moiety J chain the oligosaccharide moiety PUBTATOR J chain 3512 The complete structure of the oligosaccharide moiety of J chain isolated from a Waldenströms macroglobulin Wa has been established. 26013384 8 12 gly O-glycosylated 997:1010 arg1 fully O-glycosylated rHu-EPO fully O-glycosylated rHu-EPO PUBTATOR EPO 2056 It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126. 26013384 8 43 gly non-O-glycosylated 943:960 arg1 non-O-glycosylated rHu-EPO non-O-glycosylated rHu-EPO PUBTATOR EPO 2056 It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126. 22159084 13 78 gly N-glycosylation 1706:1720 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 In conclusion, our data indicate that N-glycosylation of SLC26A3 is important for cell surface expression and for protection from proteolytic degradation that may contribute to the understanding of pathogenesis of congenital disorders of glycosylation. 16622833 1 46 gly glycoprotein 140:151 arg1 Human alpha1-antitrypsin Human alpha1-antitrypsin PUBTATOR Human alpha1-antitrypsin 5265 Human alpha1-antitrypsin (A1PI) is a well-known glycoprotein in human plasma important for the protection of tissues from proteolytic enzymes. 22023369 3 40 gly sFcγRIIIa 568:576 arg1 the two N-glycans FcγRIIIa the two N-glycans PUBTATOR FcγRIIIa 2214 The crystal structure shows that one of the two N-glycans of sFcγRIIIa mediates the interaction with nonfucosylated Fc, thereby stabilizing the complex. 19855092 9 97 gly desialylated 1492:1503 arg1 desialylated adiponectin desialylated adiponectin PUBTATOR adiponectin Q15848 Uptake of desialylated adiponectin by isolated primary rat hepatocytes was also accelerated, suggesting a role for the hepatic asialoglycoprotein receptor. 17117926 10 37 gly glycosylation 1338:1350 arg1 Wnt-5a Wnt-5a PUBTATOR Wnt-5a 7474 Thus the post-translational palmitoylation and glycosylation of Wnt-5a are important for the actions and secretion of Wnt-5a. 19556306 2 44 gly TLR4 291:294 arg1 the N-glycan TLR4 the N-glycan PUBTATOR TLR4 7099 The purposes of this study were to characterize the N-glycan of TLR4 and to investigate the roles of MD-2 in N-linked glycosylation and cell surface expression of TLR4. 19556306 2 66 gly glycosylation 345:357 arg1 TLR4 TLR4 PUBTATOR TLR4 7099 The purposes of this study were to characterize the N-glycan of TLR4 and to investigate the roles of MD-2 in N-linked glycosylation and cell surface expression of TLR4. 17956937 0 46 gly antigen 50:56 arg1 Oligosaccharide profiles prostate specific antigen Oligosaccharide profiles OGER prostate specific antigen P07288 Oligosaccharide profiles of the prostate specific antigen in free and complexed forms from the prostate cancer patient serum and in seminal plasma: a glycopeptide approach. 23395175 5 25 gly modified 755:762 arg3 Clock AND O-GlcNAcylation Clock O-GlcNAcylation PUBTATOR Clock 12753 Central clock proteins, Clock and Period, are reversibly modified by O-GlcNAcylation to regulate their transcriptional activities. 23395175 5 25 gly modified 755:762 arg3 Central clock proteins AND O-GlcNAcylation Central clock proteins O-GlcNAcylation PUBTATOR Central clock proteins 12753 Central clock proteins, Clock and Period, are reversibly modified by O-GlcNAcylation to regulate their transcriptional activities. 24125761 2 53 gly glycoprotein 228:239 arg1 AADAC AADAC PUBTATOR AADAC 13 AADAC is a glycoprotein, but the role of glycosylation remains unclear. 21138434 2 77 gly N-glycosylation 373:387 arg1 the 5-HT3A subunit the 5-HT3A subunit PUBTATOR 5-HT3A subunit 3359 Since N-glycosylation of the 5-HT3A subunit impacts cell surface trafficking, the presence of N-glycosylation of the human (h) 5-HT3B subunit and the influence upon cell membrane expression was investigated. 21138434 2 83 gly N-glycosylation 461:475 arg1 the human (h) 5-HT3B subunit the human (h) 5-HT3B subunit PUBTATOR 5-HT3B subunit 9177 Since N-glycosylation of the 5-HT3A subunit impacts cell surface trafficking, the presence of N-glycosylation of the human (h) 5-HT3B subunit and the influence upon cell membrane expression was investigated. 15628971 4 86 gly attached 489:496 arg1 C4ST-1 AND the N-linked oligosaccharides C4ST-1 the N-linked oligosaccharides PUBTATOR C4ST-1 314694 In the present paper, we investigated the functional role of the N-linked oligosaccharides attached to C4ST-1. 1457416 1 59 gly 2HS-glycoprotein 270:285 arg1 human plasma alpha 2HS-glycoprotein human plasma alpha 2HS-glycoprotein PUBTATOR alpha 2HS-glycoprotein 197 The structure of the glycans of the A-chain of human plasma alpha 2HS-glycoprotein was established from the chemical compositions of its derivatives prepared by sequential enzymatic degradation of the carbohydrate moiety, from the determination of the kind and amount of the monosaccharides liberated after each step of the enzymatic digestion, and from the distinct specificity of the highly purified exoglycosidases. 12654314 0 14 gly M 91:91 arg1 the N-linked oligosaccharides immunoglobulin M the N-linked oligosaccharides OGER immunoglobulin M P01872 Site-specific characterization of the N-linked oligosaccharides of a murine immunoglobulin M by high-performance liquid chromatography/electrospray mass spectrometry. 24018687 3 39 part_of DDR1 724:727 arg1 asparagine 211 DDR1 asparagine 211 PUBTATOR SpecificSite DDR1 780 asparagine 211 Site-directed mutational analysis of the consensus N-glycosylation sites of the DDRs revealed that mutations of asparagine 213 of DDR2 and asparagine 211 of DDR1, a conserved N-glycosylation site among vertebrate DDRs, inhibited the generation of the high-molecular-mass isoform. 24018687 3 53 part_of DDR2 697:700 arg1 asparagine 213 DDR2 asparagine 213 PUBTATOR SpecificSite DDR2 4921 asparagine 213 Site-directed mutational analysis of the consensus N-glycosylation sites of the DDRs revealed that mutations of asparagine 213 of DDR2 and asparagine 211 of DDR1, a conserved N-glycosylation site among vertebrate DDRs, inhibited the generation of the high-molecular-mass isoform. 27314333 4 12 gly N-glycosylated 695:708 arg1 Rspo3 Rspo3 PUBTATOR Rspo3 84870 Using chemical inhibitors, deglycosylase and site-directed mutagenesis, we found that human Rspo1 and Rspo3 are both N-glycosylated at N137, a site near the C-terminus of the furin repeat 2 domain, and Rspo2 is N-glycosylated at N160, a position near the N-terminus of TSR1 domain. 27314333 4 12 gly N-glycosylated 695:708 arg1 human Rspo1 human Rspo1 PUBTATOR Rspo1 284654 Using chemical inhibitors, deglycosylase and site-directed mutagenesis, we found that human Rspo1 and Rspo3 are both N-glycosylated at N137, a site near the C-terminus of the furin repeat 2 domain, and Rspo2 is N-glycosylated at N160, a position near the N-terminus of TSR1 domain. 27314333 4 50 gly N-glycosylated 789:802 arg1 Rspo2 Rspo2 PUBTATOR Rspo2 340419 Using chemical inhibitors, deglycosylase and site-directed mutagenesis, we found that human Rspo1 and Rspo3 are both N-glycosylated at N137, a site near the C-terminus of the furin repeat 2 domain, and Rspo2 is N-glycosylated at N160, a position near the N-terminus of TSR1 domain. 21550978 6 27 gly glycosylated 1166:1177 arg1 hPAR(1) hPAR(1) PUBTATOR hPAR(1) 2149 In addition, hPAR(1) is heavily N-linked glycosylated and sialylated in epithelial cell lines, and glycosylation occurs at all five consensus sites, namely, Asn(35), Asn(62), Asn(75), Asn(250), and Asn(259). 21550978 6 83 gly sialylated 1183:1192 arg1 hPAR(1) hPAR(1) PUBTATOR hPAR(1) 2149 In addition, hPAR(1) is heavily N-linked glycosylated and sialylated in epithelial cell lines, and glycosylation occurs at all five consensus sites, namely, Asn(35), Asn(62), Asn(75), Asn(250), and Asn(259). 9757569 1 7 part_of angiotensinogen 165:179 arg1 Ser14 angiotensinogen Ser14 PUBTATOR AminoAcid angiotensinogen P01019 Ser14 A mutant angiotensinogen, S14N, in which Ser14 of ovine angiotensinogen was replaced by Asn to form a N-glycosylation site, was produced in CHO cells. 10029548 0 68 gly glycosylated 144:155 arg1 human transferrin human transferrin PUBTATOR transferrin 7018 X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32. 9136890 1 78 gly has 137:139 arg1 TfR AND three N-linked oligosaccharides TfR three N-linked oligosaccharides PUBTATOR TfR 7037 The human transferrin receptor (TfR) has three N-linked oligosaccharides. 9136890 1 78 gly has 137:139 arg1 The human transferrin receptor AND three N-linked oligosaccharides The human transferrin receptor three N-linked oligosaccharides PUBTATOR transferrin receptor 7037 The human transferrin receptor (TfR) has three N-linked oligosaccharides. 7538124 5 67 gly glycopeptides 941:953 arg1 K18 K18 PUBTATOR K18 3875 We identified the major glycosylation sites of K18 by comparing the tryptic 3H-glycopeptide pattern of the panel of mutant and wild type K18 expressed in the insect cells with the glycopeptides of K18 in human colonic cells. 7538124 5 79 gly glycosylation 785:797 arg1 K18 K18 PUBTATOR K18 3875 We identified the major glycosylation sites of K18 by comparing the tryptic 3H-glycopeptide pattern of the panel of mutant and wild type K18 expressed in the insect cells with the glycopeptides of K18 in human colonic cells. 16212939 4 55 part_of IL-1ra 822:827 arg1 Asn84 IL-1ra Asn84 PUBTATOR AminoAcid IL-1ra 3557 Asn84 The mutation of potential N-glycosylation site, by substituting Gln for either Asn7 of N-terminal 24 amino acids of hIL-1beta (Asn7Gln) or Asn84 of IL-1ra (Asn84Gln), resulted in a dramatic reduction of rhG-CSF secretion efficiency. 16212939 4 55 part_of IL-1ra 822:827 arg1 Asn7 IL-1ra Asn7 PUBTATOR AminoAcid IL-1ra 3557 Asn7 The mutation of potential N-glycosylation site, by substituting Gln for either Asn7 of N-terminal 24 amino acids of hIL-1beta (Asn7Gln) or Asn84 of IL-1ra (Asn84Gln), resulted in a dramatic reduction of rhG-CSF secretion efficiency. 1737041 3 43 gly GM-CSF 719:724 arg1 the carbohydrate modification characteristic GM-CSF the carbohydrate modification characteristic PUBTATOR GM-CSF 1437 Previous studies have failed to detect a significant functional role for the carbohydrate modification characteristic of human GM-CSF. 18642129 0 75 gly VII 93:95 arg1 O-glycans coagulation factor VII O-glycans OGER coagulation factor VII P08709 Mass spectrometric characterization of N- and O-glycans of plasma-derived coagulation factor VII. 18642129 0 75 gly VII 93:95 arg1 N- coagulation factor VII N- OGER coagulation factor VII P08709 Mass spectrometric characterization of N- and O-glycans of plasma-derived coagulation factor VII. 23209641 3 20 gly glycosylation 547:559 arg1 adiponectin adiponectin PUBTATOR adiponectin Q15848 Hydroxylation and, especially, glycosylation of the lysine residues of adiponectin have been shown to be essential for the formation of the more active high molecular weight adiponectin oligomers and thus for its function. 22688517 5 67 gly LOX-1 995:999 arg1 N-glycans structures LOX-1 N-glycans structures PUBTATOR LOX-1 4973 Here, an approach using nonspecific protease (Pronase E) digestion followed by MALDI-QIT-TOF MS and multistage MS (MS(3)) analysis is explored to obtain site-specific N-glycosylation information of recombinant human LOX-1, in combination with glycan structure confirmation through characterizing released glycans using tandem MS. The results reveal that N-glycans structures as well as their corresponding attached site of LOX-1 can be identified simultaneously by direct MS analysis of glycopeptides from non-specific protease digestion. 7512967 8 46 gly O-glycosylation 1225:1239 arg1 IgG2b IgG2b PUBTATOR IgG2b 16016 A therapeutic significance of the O-glycosylation of IgG2b is briefly discussed. 1533633 0 64 gly glycosylation 16:28 arg1 the alpha-subunit the alpha-subunit OGER subunit P06865 Analysis of the glycosylation and phosphorylation of the alpha-subunit of the lysosomal enzyme, beta-hexosaminidase A, by site-directed mutagenesis. 19299457 4 8 part_of TSHR 624:627 arg1 TSHR hinge region residues 261-289 TSHR TSHR hinge region residues 261-289 PUBTATOR SpecificSite TSHR 25360 residues 261-289 To characterize further the CS-17 epitope, we exploited the observation that CS-17 does not recognize a chimeric receptor with TSHR hinge region residues 261-289 replaced with homologous rat LH receptor residues (13 mismatches). 8323280 7 65 gly Desialylated 1524:1535 arg1 Desialylated rhLT Desialylated rhLT Cterm Desialylated rhLT Desialylated rhLT showed a lectin-like binding character to uromodulin similar to that of tumor necrosis factor, although intact rhLT did not. 24417605 7 21 gly apolipoprotein 1058:1071 arg1 fetuin A. GM3 apolipoprotein CIII fetuin A. GM3 PUBTATOR apolipoprotein CIII 345 The observed O-glycans were all sialylated, and most contained a core 1 structure with two Neu5Acs, including those that were associated with apolipoprotein CIII (ApoC-III) and fetuin A. GM3 (monosialoganglioside, NeuAc2-3Gal1-4Glc-Cer) and GD3 (disialoganglioside, NeuAc2-8NeuAc2-3Gal1-4Glc-Cer) were the major gangliosides in HDL. 24334224 0 37 gly glycosylation 9:21 arg1 recombinant human granulocyte colony-stimulating factor recombinant human granulocyte colony-stimulating factor PUBTATOR granulocyte colony-stimulating factor 1440 O-linked glycosylation analysis of recombinant human granulocyte colony-stimulating factor produced in glycoengineered Pichia pastoris by liquid chromatography and mass spectrometry. 20581009 3 31 gly Endocan 567:573 arg1 the glycosaminoglycan (GAG) chain Endocan the glycosaminoglycan (GAG) chain PUBTATOR Endocan 11082 In this work, we characterized the glycosaminoglycan (GAG) chain of Endocan, purified either from the naturally producing human umbilical vein endothelial cells (HUVEC) or from a recombinant over-expression system in human embryonic kidney cells (HEK). 1380064 5 93 part_of found 904:908 arg2 EoCP-2 AND the residues 4 and 5 EoCP-2 the residues 4 and 5 OGER SpecificSite EoCP P13501 residues 4 and 5 In the major Eo chemotaxin, EoCP-1, the residues 4 and 5, which in EoCP-2 were found to be serine residues, could not be identified. 3567160 1 18 gly G 190:190 arg1 oligosaccharides immunoglobulin G oligosaccharides Cterm immunoglobulin G The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. 8670172 9 73 gly chain 1339:1343 arg1 Asn-18 Asn-18 Asn-18 SpecificSite Asn-18 The N-linked carbohydrate side chain of CD59u (at Asn-18) also displayed considerable heterogeneity. 8670172 9 92 gly CD59u 1348:1352 arg1 The N-linked carbohydrate side chain CD59u The N-linked carbohydrate side chain PUBTATOR CD59u 966 The N-linked carbohydrate side chain of CD59u (at Asn-18) also displayed considerable heterogeneity. 8223648 3 30 gly N-glycosylated 335:348 arg1 Half Half OGER Half Q9UNN4 Half of the protein purified by immunoaffinity chromatography was shown to be N-glycosylated at the same site as the natural IFN-omega 1. 20044576 3 18 gly glycan 562:567 arg1 recombinant alpha-DG DG, glycan PUBTATOR DG, 1605 Using mass spectrometry- and nuclear magnetic resonance (NMR)-based structural analyses, we identified a phosphorylated O-mannosyl glycan on the mucin-like domain of recombinant alpha-DG, which was required for laminin binding. 7613477 5 51 part_of LCAT 1156:1159 arg1 Asn384 LCAT Asn20, Asn84, Asn272, and Asn384 PUBTATOR AminoAcid LCAT 3931 Asn20, Asn84, Asn272, and Asn384 All four potential N-linked glycosylation sites (Asn20, Asn84, Asn272, and Asn384) of LCAT were determined to contain sialylated triantennary and/or biantennary complex structures. 7613477 5 51 part_of LCAT 1156:1159 arg1 Asn84 LCAT Asn20, Asn84, Asn272, and Asn384 PUBTATOR AminoAcid LCAT 3931 Asn20, Asn84, Asn272, and Asn384 All four potential N-linked glycosylation sites (Asn20, Asn84, Asn272, and Asn384) of LCAT were determined to contain sialylated triantennary and/or biantennary complex structures. 7613477 5 51 part_of LCAT 1156:1159 arg1 Asn84 LCAT Asn20, Asn84, Asn272, and Asn384 PUBTATOR AminoAcid LCAT 3931 Asn20, Asn84, Asn272, and Asn384 All four potential N-linked glycosylation sites (Asn20, Asn84, Asn272, and Asn384) of LCAT were determined to contain sialylated triantennary and/or biantennary complex structures. 9524075 8 68 gly non-glycosylated 870:885 arg1 non-glycosylated procathepsin S non-glycosylated procathepsin S Cterm non-glycosylated procathepsin S 1520 In vitro processing of glycosylated as well as of non-glycosylated procathepsin S gave fully active enzymes thus indicating that the oligosaccharide chain was not necessary for proper folding. 12731890 1 47 gly glycoprotein 137:148 arg1 The epidermal growth factor receptor The epidermal growth factor receptor PUBTATOR epidermal growth factor receptor 1956 The epidermal growth factor receptor is a transmembrane glycoprotein that mediates the cellular responses to epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). 10092871 6 8 gly MMP-1 1001:1005 arg1 the N-glycan structures MMP-1 the N-glycan structures PUBTATOR MMP-1 4312 Using strategies based on sequential exoglycosidase digestion combined with matrix-assisted laser desorption ionization-time of flight MS and electrospray tandem MS, we have characterized the N-glycan structures of MMP-1, derived from human dermal fibroblasts and from the HT-1080 fibrosarcoma cell line. 11741940 8 32 gly moiety 1571:1576 arg1 Asn Asn Asn SpecificSite Asn(172) Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 11741940 8 32 gly moiety 1571:1576 arg1 172 172 172 SpecificSite Asn(172) Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 11741940 8 87 gly contains 1530:1537 arg1 sFRP-1 AND a relatively large carbohydrate moiety sFRP-1 a relatively large carbohydrate moiety PUBTATOR sFRP-1 6422 Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 21615908 5 35 gly glycosylated 891:902 arg1 Fibin Fibin PUBTATOR Fibin 67606 Fibin is an evolutionarily conserved protein, carries a cleavable signal peptide (amino acids 1-18) and is glycosylated at Asn30. 18533687 10 28 gly N-glycosylation 1631:1645 arg1 optimal intracellular cAMP signaling optimal intracellular cAMP signaling OGER cAMP Q96JM3 In particular, N-glycosylation at Asn-303 of RXFP1 was required for optimal intracellular cAMP signaling. 16332679 13 22 gly O-glycosylation 2075:2089 arg1 Sp1 Sp1 OGER Sp1 P08047 Thus, O-glycosylation of Sp1 appears to be critical for its localization into the nucleus, where it undergoes obligatory phosphorylation that is needed for Sp1 to activate calmodulin gene expression. 8942648 3 86 part_of residues 401:408 arg1 Peptide Peptide residues OGER SpecificSite Peptide residues 1 Peptide, disulfide, and glycosylation mapping of human TPO from residues 1 to 246 has been carried out using liquid chromatography-electrospray mass spectrometry (LC-ESMS).