doc_id sent_index relation_id relation trigger trigger_offset arg_num arg_base_np arg_protein arg_domain arg_site arg_sugar PSource SiteSource NProtein NID SiteName sent_text 20147410 6 68 gly rNKp46s 872:878 arg1 different sialic acid alpha2,3 and alpha2,6 linkage preferences rNKp46s different sialic acid alpha2,3 and alpha2,6 linkage preferences PUBTATOR rNKp46s 117547 We then characterized the O-glycan sequences that mediate the interaction of rNKp46 and IV H1N1; we employed rNKp46s with dissimilar glycosylation patterns and IV H1N1 strains with different sialic acid alpha2,3 and alpha2,6 linkage preferences. 29626154 0 84 gly O-glycosylation 33:47 arg1 osteopontin osteopontin PUBTATOR osteopontin 6696 Biological role of site-specific O-glycosylation in cell adhesion activity and phosphorylation of osteopontin. 29426894 3 54 gly glycoproteins 505:517 arg1 recombinant FcγRIII glycoproteins recombinant FcγRIII glycoproteins PUBTATOR FcγRIII glycoproteins 2214 Although several reports describe N-glycosylation profiles of recombinant FcγRIII glycoproteins, much remains unknown regarding their native glycoforms. 29426894 3 60 gly N-glycosylation 457:471 arg1 recombinant FcγRIII glycoproteins recombinant FcγRIII glycoproteins PUBTATOR FcγRIII glycoproteins 2214 Although several reports describe N-glycosylation profiles of recombinant FcγRIII glycoproteins, much remains unknown regarding their native glycoforms. 1700763 4 8 gly glycoprotein 624:635 arg1 the CD53 glycoprotein the CD53 glycoprotein PUBTATOR CD53 glycoprotein 963 It is suggested that the CD53 glycoprotein is the true human homologue of the rat OX-44 antigen, rather than the CD37 antigen of more restricted expression and lower NH2-terminal sequence similarity to OX-44. 22245686 0 31 gly glycosylated 93:104 arg1 glycosylated BMP-2 glycosylated BMP-2 PUBTATOR BMP-2 650 Non-glycosylated BMP-2 can induce ectopic bone formation at lower concentrations compared to glycosylated BMP-2. 22245686 0 46 gly Non-glycosylated 0:15 arg1 Non-glycosylated BMP-2 Non-glycosylated BMP-2 PUBTATOR BMP-2 650 Non-glycosylated BMP-2 can induce ectopic bone formation at lower concentrations compared to glycosylated BMP-2. 24308486 2 47 gly glycosylation 364:376 arg1 IgE IgE PUBTATOR IgE 3497 Here, we present site-specific glycosylation analysis of IgE from three different sources: IgE from the serum of a hyperimmune donor, from the pooled serum of multiple nondiseased donors, and from the pooled serum of 2 patients with IgE myeloma. 12138100 11 87 gly N-glycosylation 1949:1963 arg1 NCAM NCAM PUBTATOR NCAM 4684 These combined results indicate that the synergistic effect of ST8Sia II and ST8Sia IV is caused by: 1) the ability of ST8Sia IV to add polysialic acid to oligosialic acid formed by ST8Sia II, 2) the potential of ST8Sia IV to act on more antennas of N-glycans than ST8Sia II, and 3) the ability of ST8Sia II and ST8Sia IV in combination to act on the fifth and sixth N-glycosylation sites of NCAM. 29755357 0 38 gly N-Glycosylation 0:14 arg1 Lipocalin 2 Lipocalin 2 PUBTATOR Lipocalin 2 3934 N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting. 19129245 4 79 gly contained 922:930 arg1 CD26 AND such complex-type N-glycans CD26 such complex-type N-glycans PUBTATOR CD26 1803 Glycosidase digestion revealed that CD26 contained such complex-type N-glycans that appear to mediate the MBP binding. 19038967 4 63 gly glycosylated 872:883 arg1 fully glycosylated TPP1 fully glycosylated TPP1 Cterm TPP1 In this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing four Asn-linked N-acetylglucosamines that is indistinguishable from fully glycosylated TPP1 in terms of autocatalytic processing of the proform and enzymatic properties of the mature protease. 19038967 4 37 gly H-deglycosylated 757:772 arg1 an endoglycosidase H-deglycosylated form H-deglycosylated form of TPP1 Cterm H-deglycosylated form of TPP1 In this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing four Asn-linked N-acetylglucosamines that is indistinguishable from fully glycosylated TPP1 in terms of autocatalytic processing of the proform and enzymatic properties of the mature protease. 1737783 2 47 gly glycosylation 277:289 arg1 CD4 CD4 PUBTATOR CD4 920 There have been conflicting reports as to whether glycosylation of CD4 is required for its cell surface expression. 21768397 10 78 gly glycosylation 1573:1585 arg1 HA HA Cterm HA Moreover, reduced glycosylation of HA is likely to be an important factor associated with adaptation of human IAV to growth in mouse lung. 16013610 7 47 gly present 937:943 arg1 human AT AND Fucosylation AT Fucosylation PUBTATOR AT 462 Fucosylation was not yet reported to be present in human AT from plasma, opposite to recombinant human AT from baby hamster kidney cells, which was reported as fully core fucosylated. 29532326 10 27 gly C8β 1379:1381 arg1 all the thrombospondin-like (TSP) domains C8β. all the thrombospondin-like (TSP) domains PUBTATOR C8β. 732 Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β. 29532326 10 30 gly C8α 1371:1373 arg1 all the thrombospondin-like (TSP) domains C8α all the thrombospondin-like (TSP) domains PUBTATOR C8α 731 Additionally, we elucidated the stoichiometry of all C-mannosylation sites in all the thrombospondin-like (TSP) domains of C8α and C8β. 1324936 3 76 gly N-glycosylation 629:643 arg1 the beta subunit the beta subunit OGER subunit 100760716 To investigate this point, a receptor mutant (IR beta N1234) was obtained by stable transfection into Chinese hamster ovary cells of an IR cDNA modified by site-directed mutagenesis on the four potential N-glycosylation sites (Asn-X-Ser/Thr) of the beta subunit. 29853184 1 22 gly glycoproteins 149:161 arg1 ZP2 ZP2 PUBTATOR ZP2 7783 Human zona pellucida (ZP) matrix, a delicate network of thin interconnected filaments, is primarily composed of four glycoproteins, namely, ZP1, ZP2, ZP3, and ZP4. 29853184 1 22 gly glycoproteins 149:161 arg1 ZP1 ZP1 PUBTATOR ZP1 22917 Human zona pellucida (ZP) matrix, a delicate network of thin interconnected filaments, is primarily composed of four glycoproteins, namely, ZP1, ZP2, ZP3, and ZP4. 29853184 1 22 gly glycoproteins 149:161 arg1 ZP3 ZP3 PUBTATOR ZP3 7784 Human zona pellucida (ZP) matrix, a delicate network of thin interconnected filaments, is primarily composed of four glycoproteins, namely, ZP1, ZP2, ZP3, and ZP4. 29853184 1 22 gly glycoproteins 149:161 arg1 ZP4 ZP4 PUBTATOR ZP4 57829 Human zona pellucida (ZP) matrix, a delicate network of thin interconnected filaments, is primarily composed of four glycoproteins, namely, ZP1, ZP2, ZP3, and ZP4. 23723439 8 29 gly glycosylated 1609:1620 arg1 the sulfated α-DG the sulfated α-DG Cterm DG Q14118 Furthermore, using an in vitro enzymatic assay system, we demonstrated that the sulfated α-DG by HNK-1ST is no longer glycosylated by LARGE. 7915183 5 26 gly glycosylated 636:647 arg1 human CD2 human CD2 PUBTATOR CD2 914 RESULTS: The three-dimensional structure of the glycosylated form of domain 1 of human CD2 has been determined by NMR spectroscopy. 27236198 1 65 gly utrophin-glycoprotein 316:336 arg1 the utrophin-glycoprotein complex the utrophin-glycoprotein complex OGER utrophin P46939 The neuromuscular junction (NMJ) is enriched with glycoproteins modified with N-acetylgalactosamine (GalNAc) residues, and four nominally GalNAc-specific plant lectins have historically been used to identify the NMJ and the utrophin-glycoprotein complex. 19951703 9 12 gly glycosylation 1240:1252 arg1 UGT1A9 UGT1A9 PUBTATOR UGT1A9 54600 These results suggest that the glycosylation that occurs during translation is important for the folding of UGT1A9. 12877809 11 35 gly glycosylation 1788:1800 arg1 the tree shrew CETP the tree shrew CETP PUBTATOR CETP 100327267 CONCLUSION: The possible glycosylation in the tree shrew CETP may be involved in the molecular mechanism of its insusceptibility to atherosclerosis. 23556518 10 73 gly unglycosylated 1372:1385 arg1 the unglycosylated hTfR2 the unglycosylated hTfR2 PUBTATOR hTfR2 7036 We further provide evidence that the unglycosylated hTfR2 behaved in manner different from that of the WT in response to holo-Tf treatment. 2717620 1 24 gly glycoprotein 83:94 arg1 Saposin A Saposin A Cterm Saposin A Saposin A, a heat-stable 16-kDa glycoprotein, was isolated from Gaucher disease spleen and purified to homogeneity. 9294593 7 14 gly unglycosylated 1220:1233 arg1 unglycosylated rBPI unglycosylated rBPI Cterm rBPI P17213 Most sera recognized nBPI, rBPI and unglycosylated rBPI equally suggesting that glycosylation has no influence on antigen recognition. 9135025 12 122 gly glycosylation 1929:1941 arg1 CD97 CD97 PUBTATOR CD97 976 SW 1736, HTh 74, and 8505 C cells apparently expressed CD97 with alternative glycosylation compared to peripheral lymphocytes, whereas most of the CD97 antigen presented on thyrocytes and C 643 cells had glycosylation sites resembling those of lymphocytes. 21374492 2 7 gly glycoprotein 262:273 arg1 gp41 (1) gp41 (1) Cterm gp41 The polypeptide precursor gp160 of HIV-1 forms the external glycoprotein, gp120 and the transmembrane glycoprotein, gp41 (1). 21374492 2 52 gly glycoprotein 220:231 arg1 gp120 gp120 PUBTATOR gp120 155971 The polypeptide precursor gp160 of HIV-1 forms the external glycoprotein, gp120 and the transmembrane glycoprotein, gp41 (1). 27268051 8 47 gly sites 1322:1326 arg1 Notch Notch sites PUBTATOR Notch 31293 Although the current putative consensus sequence for O-GlcNAcylation predicts 18 O-GlcNAc sites on Notch, we only observed apparent O-GlcNAc modification at five sites. 17307740 0 16 gly chain 36:40 arg1 autotaxin autotaxin chain PUBTATOR autotaxin 5168 An essential oligomannosidic glycan chain in the catalytic domain of autotaxin, a secreted lysophospholipase-D. 14702339 1 72 gly mutations 251:259 arg1 TPP I TPP I mutations PUBTATOR TPP I 1200 Naturally occurring mutations in TPP I are associated with the classic late infantile neuronal ceroid lipofuscinosis. 22006924 0 68 gly gp120 55:59 arg1 The highly conserved glycan HIV-1 gp120 The highly conserved glycan PUBTATOR HIV-1 gp120 155971 The highly conserved glycan at asparagine 260 of HIV-1 gp120 is indispensable for viral entry. 9557657 3 66 gly glycoprotein 642:653 arg1 The havcr-1 glycoprotein The havcr-1 glycoprotein PUBTATOR havcr-1 glycoprotein 26762 The havcr-1 glycoprotein contains four putative N-glycosylation sites, two in the Cys-rich region and two in the TSP-rich region. 19166345 12 10 gly glycosylation 2002:2014 arg1 intact hH(4)R intact hH(4)R PUBTATOR hH(4)R 59340 In conclusion, (i) hH(4)R shows high constitutive activity and structural instability; (ii) hH(4)R shows a G-protein-independent high-affinity state; (iii) hH(4)R conformation is stabilized by agonists, inverse agonists and G-proteins; (iv) hH(4)R glycosylation is essential for cell-surface expression of intact hH(4)R. 12242028 0 66 gly glycosylation 48:60 arg1 the chorionic gonadotropin beta subunit the chorionic gonadotropin beta subunit PUBTATOR chorionic gonadotropin beta subunit 1082 Unmasking a new recognition signal for O-linked glycosylation in the chorionic gonadotropin beta subunit. 17195076 10 55 gly glycosylation 1491:1503 arg1 MUC1 MUC1 OGER MUC1 P15941 The detection of MGL positive cells in situ at the tumor site together with the modified glycosylation status of MUC1 to target MGL on DC suggests that MGL positive antigen presenting cells may play a role in tumor progression. 29944110 9 61 part_of HA1 1432:1434 arg1 residue 158 HA1 residue 158 PUBTATOR SpecificSite HA1 23526 residue 158 Through a mass spectrometric (MS) analysis of HA, the glycosylated sites of HA1 were established and we determined that residue 158 of HA1 was glycosylated and so modified a neutralization-sensitive epitope. 3934016 5 13 gly N-glycosylation 980:994 arg1 human angiotensinogen human angiotensinogen PUBTATOR angiotensinogen 183 For rat angiotensinogen, only 2 of 3 potential sites of N-glycosylation were utilized; in contrast, all 4 potential sites of N-glycosylation of human angiotensinogen were utilized. 17132688 1 10 gly P-glycoprotein 212:225 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Cystic fibrosis transmembrane conductance regulator (CFTR) and P-glycoprotein (P-gp) are ATP-binding cassette (ABC) transporters that have two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs). 17132688 1 10 gly P-glycoprotein 212:225 arg1 Cystic fibrosis transmembrane conductance regulator Cystic fibrosis transmembrane conductance regulator PUBTATOR Cystic fibrosis transmembrane conductance regulator 1080 Cystic fibrosis transmembrane conductance regulator (CFTR) and P-glycoprotein (P-gp) are ATP-binding cassette (ABC) transporters that have two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs). 17132688 1 10 gly P-glycoprotein 212:225 arg1 P-gp P-gp PUBTATOR P-gp 5243 Cystic fibrosis transmembrane conductance regulator (CFTR) and P-glycoprotein (P-gp) are ATP-binding cassette (ABC) transporters that have two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs). 7524670 0 34 gly gonadotropin 69:80 arg1 N-linked oligosaccharides chorionic gonadotropin N-linked oligosaccharides OGER chorionic gonadotropin Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits. 7524670 0 102 gly beta-subunits 95:107 arg1 N-linked oligosaccharides lutropin beta N-linked oligosaccharides OGER lutropin beta Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits. 22805525 0 48 gly glycosylation 9:21 arg1 dimeric acetylcholinesterase dimeric acetylcholinesterase PUBTATOR acetylcholinesterase 43 N-linked glycosylation of dimeric acetylcholinesterase in erythrocytes is essential for enzyme maturation and membrane targeting. 29162128 1 52 gly glycosylation 118:130 arg1 HA HA Cterm HA A stem glycosylation site of hemagglutinin (HA) is important to the stability of the HA trimmer. 15373830 0 80 gly glycosylated 68:79 arg1 The tumor suppressor HIC1 The tumor suppressor HIC1 OGER HIC1 Q14526 The tumor suppressor HIC1 (hypermethylated in cancer 1) is O-GlcNAc glycosylated. 15841140 1 5 gly glycosylated 166:177 arg1 differently glycosylated IgA1 differently glycosylated IgA1 PUBTATOR IgA1 3493 OBJECTIVE: To investigate the binding capacities of differently glycosylated IgA1 on human umbilical vein endothelial cells (HUVEC). 2954816 1 2 gly glycoproteins 290:302 arg1 the antigen p150,95 the antigen p150,95 OGER p150 O95163 The lymphocyte-function-associated antigen-1 (LFA-1), the complement receptor type 3 (CR3) and the antigen p150,95 are cell-surface glycoproteins. 2954816 1 2 gly glycoproteins 290:302 arg1 The lymphocyte-function-associated antigen-1 The lymphocyte-function-associated antigen-1 PUBTATOR lymphocyte-function-associated antigen-1 3683 The lymphocyte-function-associated antigen-1 (LFA-1), the complement receptor type 3 (CR3) and the antigen p150,95 are cell-surface glycoproteins. 29932112 9 12 gly N-glycosylation 1264:1278 arg1 Panx2 Panx2 PUBTATOR Panx2 56666 Our study indicates that N-glycosylation may be important for folding and trafficking of Panx2. 3950419 4 8 gly sialoglycoprotein 889:905 arg1 GPAch GPAch Cterm GPAch 2993 Although human MN blood group phenotype results from an amino acid polymorphism of GPA, Western blots showed that in chimpanzee sialoglycoprotein (GPAch) always expresses the M blood group, whereas chimpanzee sialoglycoprotein (GPBch) expresses either the N blood group or a null phenotype. 3950419 4 105 gly sialoglycoprotein 970:986 arg1 GPBch GPBch Cterm GPBch 2994 Although human MN blood group phenotype results from an amino acid polymorphism of GPA, Western blots showed that in chimpanzee sialoglycoprotein (GPAch) always expresses the M blood group, whereas chimpanzee sialoglycoprotein (GPBch) expresses either the N blood group or a null phenotype. 7827124 0 62 gly glycosylation 17:29 arg1 lecithin:cholesterol acyltransferase lecithin:cholesterol acyltransferase OGER lecithin:cholesterol acyltransferase P04180 Role of N-linked glycosylation of lecithin:cholesterol acyltransferase in lipoprotein substrate specificity. 6164735 1 2 gly sialoglycoprotein 82:98 arg1 The major erythrocyte membrane (MN) sialoglycoprotein The major erythrocyte membrane (MN) sialoglycoprotein OGER MN) sialoglycoprotein P02724 The major erythrocyte membrane (MN) sialoglycoprotein in Mg red cells was found to exhibit a slightly decreased sodium-dodecyl-sulphate polyacrylamide gel electrophoretic molecular weight and periodic and/Schiff staining intensity. 12950230 10 97 gly O-glycosylation 1734:1748 arg1 recombinant MUC1 recombinant MUC1 PUBTATOR MUC1 100772836 This is the first reported production of large quantities of recombinant MUC1 with a breast cancer-like O-glycosylation that could be used for the immunotherapy of breast cancer. 1722368 7 64 gly determinants 738:749 arg1 GPA GPA determinants PUBTATOR GPA 2993 Serological and immunochemical studies with human and monoclonal antibodies to various determinants on glycophorin A (GPA) suggest that Mi.IX is associated with an aberrant GPA molecule that lacks the trypsin cleavage site at amino-acid residue 39, retains the chymotrypsin cleavage site at residue 34 and has an apparent Mr of about 1,000 less than normal GPA. 1722368 7 64 gly determinants 738:749 arg1 glycophorin A glycophorin A determinants PUBTATOR glycophorin A 2993 Serological and immunochemical studies with human and monoclonal antibodies to various determinants on glycophorin A (GPA) suggest that Mi.IX is associated with an aberrant GPA molecule that lacks the trypsin cleavage site at amino-acid residue 39, retains the chymotrypsin cleavage site at residue 34 and has an apparent Mr of about 1,000 less than normal GPA. 19254717 3 10 gly N-glycosylation 588:602 arg1 a heavily glycosylated neuroglycoprotein Lingo-1 a heavily glycosylated neuroglycoprotein Lingo-1 PUBTATOR Lingo-1 84894 Applying this approach results in a quick identification of essential N-glycosylation sites of a heavily glycosylated neuroglycoprotein Lingo-1, which are sufficient for the support of its surface expression. 19254717 3 44 gly glycosylated 623:634 arg1 a heavily glycosylated neuroglycoprotein Lingo-1 a heavily glycosylated neuroglycoprotein Lingo-1 PUBTATOR Lingo-1 84894 Applying this approach results in a quick identification of essential N-glycosylation sites of a heavily glycosylated neuroglycoprotein Lingo-1, which are sufficient for the support of its surface expression. 17957771 0 46 gly receptor 38:45 arg1 N-linked glycans insulin receptor N-linked glycans PUBTATOR insulin receptor 3643 N-linked glycans of the human insulin receptor and their distribution over the crystal structure. 17956937 6 70 gly predominant 1207:1217 arg2 the seminal plasma PSA AND high-mannose and hybrid types the seminal plasma PSA high-mannose and hybrid types PUBTATOR PSA 354 In the seminal plasma PSA, high-mannose and hybrid types of oligosaccharides were predominant, and the sialic acids attached to the latter as well as to biantennary oligosaccahrides were exclusively alpha2,6-linked because they were removed by Arthrobacter ureafaciens neuraminidase but resistant to S. pneumoniae neuraminidase. 7510249 5 31 gly glycosylation 911:923 arg1 K18 K18 PUBTATOR K18 3875 The glycosylation and phosphorylation of K18 in human and insect cells were very similar as determined by tryptic peptide mapping and localization to the head and proximal rod domains. 28025250 1 21 gly glycosylation 209:221 arg1 the glycoprotein Mucin 1 (MUC1) the glycoprotein Mucin 1 (MUC1) PUBTATOR MUC1 4582 In cancer cells, the glycoprotein Mucin 1 (MUC1) undergoes abnormal, truncated glycosylation. 7781780 1 4 part_of erythropoietin 285:298 arg1 Asn-24 erythropoietin Asn-24 PUBTATOR SpecificSite erythropoietin 2056 Asn-24 A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. 17390031 9 27 part_of WNT8B 1453:1457 arg1 two Asn-linked glycosylation sites WNT8B two Asn-linked glycosylation sites PUBTATOR AminoAcid WNT8B 7479 sites, Gly230, and Arg284 Comparative proteomics revealed that N-terminal signal peptide, 22 Cys residues, two Asn-linked glycosylation sites, Gly230, and Arg284 of human WNT8B were conserved among mammalian WNT8B orthologs. 10995746 8 5 gly fXa 1185:1187 arg1 the new oligosaccharide Q333N fXa the new oligosaccharide PUBTATOR Q333N fXa 2159 N-Glycanase cleaves the new oligosaccharide from Q333N fXa leaving aspartic acid. 17500062 5 68 gly glycosylation 799:811 arg1 OPN OPN PUBTATOR OPN 20750 We have characterized the complete phosphorylation and glycosylation patterns of OPN expressed by murine ras-transformed fibroblasts (FbOPN) and differentiating osteoblasts (ObOPN) by a combination of mass spectrometric analyses and Edman degradation. 19880513 11 37 gly glycoproteins 2052:2064 arg1 OPN OPN PUBTATOR OPN 20750 Our study presents a novel and systematic approach for identification of isoform-specific substrates of the ppGalNAcT family and suggests ppGalNAcT-1 to be indispensable for O-glycosylation at specific sites of the bone glycoproteins OPN and BSP. 19880513 11 37 gly glycoproteins 2052:2064 arg1 BSP BSP Cterm BSP Our study presents a novel and systematic approach for identification of isoform-specific substrates of the ppGalNAcT family and suggests ppGalNAcT-1 to be indispensable for O-glycosylation at specific sites of the bone glycoproteins OPN and BSP. 25580119 7 42 part_of IRS-1 1326:1330 arg1 IRS-1 Ser-312 IRS-1 Ser-312, 984, 1037, and 1101 PUBTATOR SpecificSite IRS-1 3667 Ser-312, 984, 1037, and 1101 Moreover, alternative phosphorylation and O-glycosylation on IRS-1 Ser-312, 984, 1037, and 1101 may act as possible therapeutic targets to minimize the risk of AD and T2DM. 25580119 7 42 part_of IRS-1 1326:1330 arg1 1037 IRS-1 Ser-312, 984, 1037, and 1101 PUBTATOR SpecificSite IRS-1 3667 Ser-312, 984, 1037, and 1101 Moreover, alternative phosphorylation and O-glycosylation on IRS-1 Ser-312, 984, 1037, and 1101 may act as possible therapeutic targets to minimize the risk of AD and T2DM. 25580119 7 42 part_of IRS-1 1326:1330 arg1 1101 IRS-1 Ser-312, 984, 1037, and 1101 PUBTATOR SpecificSite IRS-1 3667 Ser-312, 984, 1037, and 1101 Moreover, alternative phosphorylation and O-glycosylation on IRS-1 Ser-312, 984, 1037, and 1101 may act as possible therapeutic targets to minimize the risk of AD and T2DM. 25580119 7 42 part_of IRS-1 1326:1330 arg1 1037 IRS-1 Ser-312, 984, 1037, and 1101 PUBTATOR SpecificSite IRS-1 3667 Ser-312, 984, 1037, and 1101 Moreover, alternative phosphorylation and O-glycosylation on IRS-1 Ser-312, 984, 1037, and 1101 may act as possible therapeutic targets to minimize the risk of AD and T2DM. 25580119 7 42 part_of IRS-1 1326:1330 arg1 1101 IRS-1 Ser-312, 984, 1037, and 1101 PUBTATOR SpecificSite IRS-1 3667 Ser-312, 984, 1037, and 1101 Moreover, alternative phosphorylation and O-glycosylation on IRS-1 Ser-312, 984, 1037, and 1101 may act as possible therapeutic targets to minimize the risk of AD and T2DM. 25580119 7 42 part_of IRS-1 1326:1330 arg1 1101 IRS-1 Ser-312, 984, 1037, and 1101 PUBTATOR SpecificSite IRS-1 3667 Ser-312, 984, 1037, and 1101 Moreover, alternative phosphorylation and O-glycosylation on IRS-1 Ser-312, 984, 1037, and 1101 may act as possible therapeutic targets to minimize the risk of AD and T2DM. 2140803 5 45 gly glycosylation 696:708 arg1 Ca2 Ca2 OGER Ca2 P00918 Incubation of erythrocyte membranes instead of intact erythrocytes with glucose and glucose-6-phosphate strongly suggests that only the glycosylation of the membrane inner-surface proteins can affect Ca2(+)-ATPase activity. 2140803 5 45 gly glycosylation 696:708 arg1 Ca2(+)-ATPase activity Ca2(+)-ATPase activity OGER ATPase Incubation of erythrocyte membranes instead of intact erythrocytes with glucose and glucose-6-phosphate strongly suggests that only the glycosylation of the membrane inner-surface proteins can affect Ca2(+)-ATPase activity. 29408166 9 61 gly core-fucosylated 1681:1696 arg1 core-fucosylated PSA core-fucosylated PSA PUBTATOR PSA 354 This method could be used in large patient cohorts as core-fucosylated PSA may be a diagnostic biomarker for the differentiation of prostate cancer and other prostatic diseases, such as benign prostatic hyperplasia (BPH). 23991039 10 49 gly CD4bs 1628:1632 arg1 glycans CD4 glycans PUBTATOR CD4 920 High-resolution analyses of trimeric Env that show the orientation of glycans and polymorphic elements of the CD4bs that affect binding to antibodies like 1F7 are desirable to understand how to promote immunogenicity of more conserved elements of the CD4bs. 29580922 8 29 part_of sialylated 1351:1360 arg1 tri-antennary and sialylated N-glycans haptoglobin tri-antennary and sialylated N-glycans PUBTATOR AminoAcid haptoglobin 3240 Asn207 and Asn211 Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs. 29580922 8 60 part_of tri-antennary 1333:1345 arg1 tri-antennary and sialylated N-glycans haptoglobin tri-antennary and sialylated N-glycans PUBTATOR AminoAcid haptoglobin 3240 Asn207 and Asn211 Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs. 28815695 7 37 part_of present 1351:1357 arg2 CD59 AND the His44 residue CD59 the His44 residue PUBTATOR AminoAcid CD59 966 His44 residue Since (1) the His44 residue is not present in CD59 from other animal species and (2) humans are particularly prone to develop complications of diabetes, our results indicate that the Lys41 /His44 glycation motif in human CD59 may confer humans a higher risk of developing vascular disease in response to hyperglycemia. 28815695 7 78 part_of CD59 1362:1365 arg1 the His44 residue CD59 the His44 residue PUBTATOR AminoAcid CD59 966 His44 residue Since (1) the His44 residue is not present in CD59 from other animal species and (2) humans are particularly prone to develop complications of diabetes, our results indicate that the Lys41 /His44 glycation motif in human CD59 may confer humans a higher risk of developing vascular disease in response to hyperglycemia. 15658935 0 69 gly glycoforms 41:50 arg1 PrP PrP PUBTATOR PrP 281432 Separation of native prion protein (PrP) glycoforms by copper-binding using immobilized metal affinity chromatography (IMAC). 28801655 3 27 gly O-glycosylation 584:598 arg1 Ser37/Ser41 Ser37/Ser41 PUBTATOR 1 10678 This study identifies β1AR N-terminal O-glycosylation at Ser37/Ser41 as a mechanism that prevents β1AR N-terminal cleavage. 19478079 0 1 gly modification 29:40 arg1 CCAAT enhancer-binding protein beta CCAAT enhancer-binding protein beta modification PUBTATOR CCAAT enhancer-binding protein beta 1051 O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation. 8636209 7 68 gly glycosylation 864:876 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 Mutagenesis of N-linked glycosylation sites showed that glycosylation of CD3 gamma is not required for TCR assembly and expression. 8636209 7 68 gly glycosylation 864:876 arg1 CD3 gamma CD3 gamma PUBTATOR CD3 gamma 917 Mutagenesis of N-linked glycosylation sites showed that glycosylation of CD3 gamma is not required for TCR assembly and expression. 23422691 2 15 gly glycoprotein 274:285 arg1 VEGF VEGF PUBTATOR VEGF 7422 VEGF is a homodimeric glycoprotein that contains one N-glycosylation site. 20477988 3 14 gly VIP36 367:371 arg1 the sugar-binding properties VIP36 the sugar-binding properties PUBTATOR VIP36 10960 Although the sugar-binding properties of VIP36 in vitro have been characterized in detail, the function of VIP36 in the intact cell remains unclear as no convincing glycoprotein cargo has been identified. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr81 Thr81 Thr81 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Ser158 Ser158 Ser158 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Ser160 Ser160 Ser160 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr86 Thr86 Thr86 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr156 Thr156 Thr156 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 15 gly hexasaccharides 1452:1466 arg1 Thr2 Thr2 Thr2 AminoAcid Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 58 gly O-glycosylated 1384:1397 arg1 sIL-15Rα sIL-15Rα OGER sIL Q15468 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 26563299 9 97 gly contained 1331:1339 arg1 IL-15 AND no O-glycans IL-15 no O-glycans PUBTATOR IL-15 3600 IL-15 contained no O-glycans, whereas sIL-15Rα was heavily O-glycosylated with partially sialylated core 1 and 2-type mono- to hexasaccharides on Thr2, Thr81, Thr86, Thr156, Ser158, and Ser160. 9774483 1 20 gly acid 186:189 arg1 NCAM NCAM acid PUBTATOR NCAM 4684 PST and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (NCAM), and these two polysialyltransferases often exist together in the same tissues. 9774483 1 20 gly acid 186:189 arg1 neural cell adhesion molecule neural cell adhesion molecule acid PUBTATOR neural cell adhesion molecule 4684 PST and STX are polysialyltransferases that form polysialic acid in the neural cell adhesion molecule (NCAM), and these two polysialyltransferases often exist together in the same tissues. 12458951 9 90 gly glucose 1336:1342 arg1 Asn439 Asn439 Asn439 AminoAcid Asn324 and Asn439 We were able to identify partial beta-hydroxylation on Asn324 and Asn439, and O-linked glucose on Ser287 from the peptide/glycopeptide map and their mass spectra. 12458951 9 90 gly glucose 1336:1342 arg1 Ser287 Ser287 Ser287 AminoAcid Ser287 We were able to identify partial beta-hydroxylation on Asn324 and Asn439, and O-linked glucose on Ser287 from the peptide/glycopeptide map and their mass spectra. 12458951 9 90 gly glucose 1336:1342 arg1 Asn324 Asn324 Asn324 AminoAcid Asn324 and Asn439 We were able to identify partial beta-hydroxylation on Asn324 and Asn439, and O-linked glucose on Ser287 from the peptide/glycopeptide map and their mass spectra. 22726956 4 68 gly low-glycosylated 720:735 arg1 a recombinant low-glycosylated human BChE a recombinant low-glycosylated human BChE PUBTATOR BChE 590 Conversion of BChE into a catalytic bioscavenger by rational design or designing reactivators specific to BChE required structural data obtained using a recombinant low-glycosylated human BChE expressed in Chinese hamster ovary cells. 17563389 2 34 gly glycosylation 324:336 arg1 the NK1R the NK1R PUBTATOR NK1R 6869 We studied the role of N-linked glycosylation in the functioning of the NK1R by constructing three receptor mutants: two single mutants (Asn --> Gln-14 and Asn --> Gln-18) and a double mutant, lacking both glycosylation sites. 17609437 9 33 gly sialylated 1349:1358 arg1 Large free GPHalpha Large free GPHalpha PUBTATOR GPHalpha 1081 Large free GPHalpha and GPHalpha alpha homodimers were more rapidly sialylated than hCG alphabeta-heterodimers indicating a sequestration mechanism in the secretory pathway. 17609437 9 33 gly sialylated 1349:1358 arg1 GPHalpha alpha GPHalpha alpha PUBTATOR GPHalpha alpha homodimers 1081 Large free GPHalpha and GPHalpha alpha homodimers were more rapidly sialylated than hCG alphabeta-heterodimers indicating a sequestration mechanism in the secretory pathway. 11437595 7 87 gly glycoprotein 1915:1926 arg1 platelet glycoprotein Ibalpha platelet glycoprotein Ibalpha PUBTATOR glycoprotein Ibalpha 2811 The calmodulin tag was also exploited in the development of assays to measure directly vWf and thrombin binding, since it did not interfere with either, demonstrating the feasibility for the use of this soluble receptor fusion protein in detailed biophysical assays to investigate the molecular mode of binding of platelet glycoprotein Ibalpha to these ligands. 2737288 0 9 gly glycosylation 41:53 arg1 human pancreatic elastase 1 human pancreatic elastase 1 PUBTATOR pancreatic elastase 1 1990 Localization and characterization of the glycosylation site of human pancreatic elastase 1. 27612916 7 87 gly glycan 1311:1316 arg1 the UGT2B7 enzyme UGT2B7 enzyme glycan PUBTATOR UGT2B7 enzyme 7364 The presence of an additional N-linked glycan on the UGT2B7 enzyme, likely affecting proper protein folding, resulted in a significant decrease of 49% and 40% in the formation of zidovudine and mycophenolic acid glucuronides, respectively. 26018173 9 9 gly glycosylation 1730:1742 arg1 membrane-associated Env membrane-associated Env PUBTATOR Env 100616444 Additionally, we report a newly observed O-linked glycosylation site, T606, and we show that the full O-linked glycosylation profile of membrane-associated Env is similar to that of soluble gp140. 27314333 0 47 gly N-Glycosylation 0:14 arg1 Human R-Spondin 1 Human R-Spondin 1 PUBTATOR Human R-Spondin 1 284654 N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability. 10212215 4 75 gly glycosylated 731:742 arg1 full-length glycosylated and nonglycosylated tPA full-length glycosylated and nonglycosylated tPA OGER tPA P00750 To understand what structural features of tPA are involved in cell surface interactions, we performed kinetic assays with a range of tPA domain deletion mutants consisting of full-length glycosylated and nonglycosylated tPA (F-G-K1-K2-P), DeltaFtPA (G-K1-K2-P), K2-P tPA (BM 06.022 or Reteplase), and protease domain (P). 10212215 4 79 gly nonglycosylated 748:762 arg1 full-length glycosylated and nonglycosylated tPA full-length glycosylated and nonglycosylated tPA OGER tPA P00750 To understand what structural features of tPA are involved in cell surface interactions, we performed kinetic assays with a range of tPA domain deletion mutants consisting of full-length glycosylated and nonglycosylated tPA (F-G-K1-K2-P), DeltaFtPA (G-K1-K2-P), K2-P tPA (BM 06.022 or Reteplase), and protease domain (P). 9705299 1 3 gly glycoprotein 196:207 arg1 glucose-6-phosphatase glucose-6-phosphatase OGER glucose-6-phosphatase P35575 Deficiency of glucose-6-phosphatase (G6Pase), an endoplasmic reticulum transmembrane glycoprotein, causes glycogen storage disease type 1a. 8824178 2 77 gly glycosylated 379:390 arg1 a glycosylated alphabeta TCR a glycosylated alphabeta TCR Cterm TCR The x-ray structure of the complete extracellular fragment of a glycosylated alphabeta TCR was determined at 2.5 angstroms, and its orientation bound to a class I MHC-peptide (pMHC) complex was elucidated from crystals of the TCR-pMHC complex. 29441788 1 37 gly glycoprotein 96:107 arg1 Afamin Afamin OGER Afamin P43652 Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites. 10504397 2 44 part_of gp41 383:386 arg1 residues 590-620 gp41 residues 590-620 Cterm SpecificSite gp41 residues 590-620 The major interaction site has been located within the gp41 immunodominant region (residues 590-620), and a synthetic peptide overlapping residues 601-613 of gp41 (sequence GIWGCSGKLICTT) was shown to inhibit binding of gp41 to C1q in vitro (Thielens, N.M., Bally, I.M., Ebenbichler, C.F., Dierich, M.P. & Arlaud, G.J. (1993) J. Immunol. 10504397 2 65 part_of gp41 486:489 arg1 601-613 gp41 601-613 Cterm SpecificSite gp41 residues 601-613 The major interaction site has been located within the gp41 immunodominant region (residues 590-620), and a synthetic peptide overlapping residues 601-613 of gp41 (sequence GIWGCSGKLICTT) was shown to inhibit binding of gp41 to C1q in vitro (Thielens, N.M., Bally, I.M., Ebenbichler, C.F., Dierich, M.P. & Arlaud, G.J. (1993) J. Immunol. 28973932 5 12 gly glycosylation 1190:1202 arg1 hepatocyte growth factor receptor hepatocyte growth factor receptor PUBTATOR hepatocyte growth factor receptor 4233 In addition, O-Man glycosylation of IPT/TIG domains of plexins and hepatocyte growth factor receptor was not affected in TMTC KO cells, suggesting the existence of yet another O-Man glycosylation machinery. 23049768 1 3 gly glycoprotein 132:143 arg1 Factor X Factor X OGER Factor X (FX) P00742 Factor X (FX), a plasma glycoprotein playing a central role in coagulation has a long circulatory half-life compared to closely related coagulation factors. 28887379 11 29 gly glycosylation 1751:1763 arg1 HCD CID, HCD OGER CID, HCD Q9NR71 GlycoPAT is used to catalogue site-specific glycosylation on simple glycoproteins, standard protein mixtures and human plasma cryoprecipitate samples in three common MS/MS fragmentation modes: CID, HCD and ETD. 28887379 11 108 gly glycoproteins 1775:1787 arg1 CID CID, HCD OGER CID, HCD Q9NR71 GlycoPAT is used to catalogue site-specific glycosylation on simple glycoproteins, standard protein mixtures and human plasma cryoprecipitate samples in three common MS/MS fragmentation modes: CID, HCD and ETD. 30158294 16 37 gly N-glycosylation 2481:2495 arg1 SERINC5 SERINC5 PUBTATOR SERINC5 256987 Nonetheless, N-glycosylation per se is neither required for the ability of SERINC5 to inhibit HIV-1 infectivity nor for its sensitivity to antagonism by Nef. 2419904 1 57 gly glycoprotein 137:148 arg1 p97 p97 PUBTATOR p97 4241 p97 is a cell-surface glycoprotein that is present in most human melanomas but only in trace amounts in normal adult tissues. 27322084 5 34 gly glycoprotein 824:835 arg1 glycoprotein (G) gene glycoprotein (G) gene OGER glycoprotein (G P07996 According to the phylogenetic analysis of glycoprotein (G) gene, five HRSV genotypes NA1, ON1, BA9, BA-C, and CB1 were found in Chengdu. 10037148 1 2 gly glycoprotein 106:117 arg1 The myelin-associated glycoprotein The myelin-associated glycoprotein PUBTATOR myelin-associated glycoprotein 17136 The myelin-associated glycoprotein (MAG) has been proposed to be important for the integrity of myelinated axons. 10037148 1 2 gly glycoprotein 106:117 arg1 MAG MAG PUBTATOR MAG 17136 The myelin-associated glycoprotein (MAG) has been proposed to be important for the integrity of myelinated axons. 23214446 0 87 gly glycosylated 14:25 arg1 glycosylated soluble amyloid precursor protein alpha glycosylated soluble amyloid precursor protein alpha OGER amyloid precursor protein alpha P05067 Production of glycosylated soluble amyloid precursor protein alpha (sAPPalpha) in Leishmania tarentolae. 21417264 3 17 gly rhGAA 509:513 arg1 bis mannose 6-phosphate rhGAA bis mannose 6-phosphate OGER rhGAA Q6P7A9 To improve muscle targeting, Zhu et al. (1) conjugated periodate oxidized rhGAA with bis mannose 6-phosphate bearing synthetic glycans and achieved 5-fold greater potency in a murine Pompe efficacy model. 10712595 7 59 gly glycosylation 1329:1341 arg1 human antithrombin human antithrombin PUBTATOR antithrombin 462 Furthermore, the invariant third-position Ser137 at this glycosylation site of mammalian and chicken antithrombins is substituted by Thr in the salmon, a replacement that has been shown to induce full glycosylation in human antithrombin. 26029999 4 30 gly CD133 724:728 arg1 all eight potential N-glycosylation sites CD133 all eight potential N-glycosylation sites PUBTATOR CD133 8842 Here we analyzed the exact site(s) of N-glycosylation in CD133 by mass spectrometry and found that all eight potential N-glycosylation sites of CD133 could be indeed occupied by N-glycans. 26029999 4 46 gly N-glycosylation 699:713 arg1 CD133 CD133 PUBTATOR CD133 8842 Here we analyzed the exact site(s) of N-glycosylation in CD133 by mass spectrometry and found that all eight potential N-glycosylation sites of CD133 could be indeed occupied by N-glycans. 29426894 5 48 gly glycoproteins 901:913 arg1 the previously reported recombinant FcγRIII glycoproteins the previously reported recombinant FcγRIII glycoproteins PUBTATOR FcγRIII glycoproteins 2214 Our data indicate a distinct and common tendency of the glycoforms exhibited at each N-glycosylation site between the native and the previously reported recombinant FcγRIII glycoproteins. 10585852 5 26 gly tyrosinase 651:660 arg1 N-glycan processing tyrosinase N-glycan processing PUBTATOR tyrosinase 22173 We present here results on N-glycan processing of TRP-1 and tyrosinase and compare the maturation process and activity of both glycoproteins in the presence of inhibitors of the endoplasmic reticulum stages of N-glycosylation. 21712440 2 49 gly glycoprotein 411:422 arg1 APP APP OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 21712440 2 6 gly carrying 424:431 arg1 APP AND O-glycans APP O-glycans OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 21712440 2 6 gly carrying 424:431 arg1 APP AND N- APP N- OGER APP P05067 Although APP is a well-known membrane glycoprotein carrying both N- and O-glycans, nothing is known about the occurrence of released APP/Aβ glycopeptides in cerebrospinal fluid (CSF). 23263199 0 45 gly N-glycosylations 0:15 arg1 human α1,3-fucosyltransferase IX human α1,3-fucosyltransferase IX PUBTATOR fucosyltransferase IX 10690 N-glycosylations of human α1,3-fucosyltransferase IX are required for full enzyme activity. 15879434 1 7 gly glycoprotein 175:186 arg1 The CASR The CASR PUBTATOR CASR 846 The CASR, a cell surface glycoprotein expressed in parathyroid gland and kidney, is critical for maintaining extracellular calcium homeostasis. 9832436 13 89 gly glycoprotein 2006:2017 arg1 PLP-H PLP-H PUBTATOR PLP-H 59088 Only the 27-kDa protein was detected after N-glycosidase treatment, indicating that PLP-H is a glycoprotein. 19690161 0 0 gly glycans 17:23 arg1 Asn-89 Asn-89 Asn-89 SpecificSite Asn-89 Complex N-linked glycans on Asn-89 of Kaposi sarcoma herpes virus-encoded interleukin-6 mediate optimal function by affecting cytokine protein conformation. 8071374 9 62 gly glycosylation 1309:1321 arg1 the IRR the IRR PUBTATOR IRR 3645 Like receptors for insulin and IGF-I, the IRR was synthesized as a single polypeptide precursor that underwent proteolytic cleavage and glycosylation to yield an alpha subunit and a beta subunit. 24161696 9 1 gly Kv3.1b 1722:1727 arg1 N-glycans Kv3.1b N-glycans Cterm Kv3.1b GENERAL SIGNIFICANCE: Our study demonstrates that N-glycans of Kv3.1b contain information regarding the association, clustering, and distribution of Kv3.1b in the cell membrane, and furthermore that decreased occupancy caused by congenital disorders of glycosylation may alter the biological activity of Kv3.1b. 8388383 13 100 gly glycosylation 1544:1556 arg1 u-PAR u-PAR PUBTATOR u-PAR 5329 These results demonstrate that some extent of glycosylation of u-PAR is necessary for cellular transport and for molecular maturation events leading to ligand binding activity. 12954207 0 39 gly deglycosylation 26:40 arg1 HIV-1 gp120 HIV-1 gp120 PUBTATOR HIV-1 gp120 155971 Structure-based, targeted deglycosylation of HIV-1 gp120 and effects on neutralization sensitivity and antibody recognition. 18340083 4 33 gly glycosylated 576:587 arg1 Human GPIHBP1 Human GPIHBP1 PUBTATOR Human GPIHBP1 338328 Human GPIHBP1 is also glycosylated. 16716077 1 72 gly glycosylation 236:248 arg1 sTFR sTFR Cterm sTFR 7037 Production of the soluble portion of the transferrin receptor (sTFR) by baby hamster kidney (BHK) cells is described, and the effect of glycosylation on the biological function of sTFR is evaluated for the first time. 8702840 7 36 part_of NF-M 1099:1102 arg1 Ser48 NF-M Ser48 PUBTATOR AminoAcid NF-M 4741 Ser34 and Ser48 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 47 part_of NF-L 1127:1130 arg1 Ser48 NF-L Ser48 PUBTATOR AminoAcid NF-L 4747 Ser34 and Ser48 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 36 part_of NF-M 1099:1102 arg1 Thr19 NF-M Thr19 and Ser34 PUBTATOR AminoAcid NF-M 4741 Thr19 and Ser34 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 8702840 7 47 part_of NF-L 1127:1130 arg1 Thr19 NF-L Thr19 and Ser34 PUBTATOR AminoAcid NF-L 4747 Thr19 and Ser34 Beyond the earlier identified sites on NF-M and NF-L, O-GlcNAc sites on Thr19 and Ser34 of NF-M and Ser34 and Ser48 of NF-L are also determined here, all of which are localized in head domain sequences critical for filament assembly. 3402460 7 60 gly contains 1376:1383 arg1 AGP-A AND no diantennary structures AGP-A no diantennary structures PUBTATOR AGP-A 5004 With the assumption that each molecule contains five glycosylation sites, it could be shown that AGP-A contains no diantennary structures whereas AGP-B and AGP-C contain one and two diantennary structures, respectively. 3402460 7 117 gly contain 1435:1441 arg1 AGP-B AND one and two diantennary structures AGP-B one and two diantennary structures PUBTATOR AGP-B 5005 With the assumption that each molecule contains five glycosylation sites, it could be shown that AGP-A contains no diantennary structures whereas AGP-B and AGP-C contain one and two diantennary structures, respectively. 3402460 7 117 gly contain 1435:1441 arg1 AGP-C AND one and two diantennary structures AGP-C one and two diantennary structures Cterm AGP With the assumption that each molecule contains five glycosylation sites, it could be shown that AGP-A contains no diantennary structures whereas AGP-B and AGP-C contain one and two diantennary structures, respectively. 10574586 4 15 gly glycosylated 645:656 arg1 Fully glycosylated rFII Fully glycosylated rFII Cterm rFII 2147 Fully glycosylated rFII is also selectively retained and degraded in warfarin-treated H-35 cells. 25211026 6 81 gly sialylated 1055:1064 arg1 Sc Sc Cterm Sc 19122 As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc). 25211026 6 81 gly sialylated 1055:1064 arg1 PMCAb-derived PrP PMCAb-derived PrP PUBTATOR PrP 19122 As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc). 7510285 1 29 gly glycoprotein 192:203 arg1 the L6 antigen the L6 antigen PUBTATOR L6 antigen 4071 The murine monoclonal antibody (mAb) L6 recognizes an integral membrane glycoprotein that is highly expressed on lung, breast, colon, and ovarian carcinomas and is referred to as the L6 antigen. 21625599 2 14 gly glycoprotein 218:229 arg1 Human tyrosinase Human tyrosinase PUBTATOR Human tyrosinase 7299 Human tyrosinase is a transmembrane glycoprotein with six or seven bulky N-glycans exposed towards the lumen of subcellular organelles. 17050611 1 6 gly Pradimicin 178:187 arg1 a low-molecular-weight (molecular weight, 838) carbohydrate binding agent Pradimicin A a low-molecular-weight (molecular weight, 838) carbohydrate binding agent Cterm Pradimicin A Pradimicin A (PRM-A), an antifungal nonpeptidic benzonaphtacenequinone antibiotic, is a low-molecular-weight (molecular weight, 838) carbohydrate binding agent (CBA) endowed with a selective inhibitory activity against human immunodeficiency virus (HIV). 28287093 8 72 gly glycoprotein 1501:1512 arg1 HDL glycoprotein composition HDL glycoprotein composition OGER HDL glycoprotein Q9UNE0 Our results demonstrate that HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clinical groups, correlate with HDL's immunomodulatory capacity, and may be predictive of HDL's ability to protect from infection. 15279557 5 98 gly attached 1091:1098 arg1 serum albumin AND sugar residues serum albumin sugar residues OGER albumin P02768 In the last few years, MALDI-TOF-MS was applied to the investigation of glycation processes: the analyses of plasma proteins from diabetic or uremic patients allowed a precise determination of the average number of sugar residues attached to serum albumin or immunoglobulins of each patient. 18576678 4 26 gly glycoforms 568:577 arg1 ACE ACE PUBTATOR ACE 1636 Therefore, these mAbs could be used to distinguish different glycoforms of ACE expressed in different tissues or cell lines. 1476702 2 46 gly containing 300:309 arg1 this variant transferrin AND one sialic acid residue this variant transferrin one sialic acid residue PUBTATOR transferrin 24825 The principal component of this variant transferrin containing one sialic acid residue per mole of protein was separated from other forms of transferrin by anion-exchange chromatography, followed by lectin affinity chromatography. 2498325 2 37 gly glycosylation 96:108 arg1 human apolipoprotein (apo) E human apolipoprotein (apo) E PUBTATOR apolipoprotein (apo) E 348 The glycosylation of human apolipoprotein (apo) E was examined with purified plasma apoE and apoE produced by transfected cell lines. 17117926 9 29 gly glycosylation 1189:1201 arg1 Wnt-5a Wnt-5a PUBTATOR Wnt-5a 7474 In contrast, glycosylation was necessary for the secretion of Wnt-5a, but not essential for the actions of Wnt-5a. 27048837 9 22 gly glycosylation 1088:1100 arg1 the protein the protein OGER protein can P35658 Post translational glycosylation of the protein can also be identified. 30208353 8 87 gly glycosylation 1334:1346 arg1 ER-α ER-α PUBTATOR ER-α 2099 We also obtained evidences showing that the glycosylation of ER-α at S573 by GALNT6 is essential for protein stability and nuclear localization of ER-α in breast cancer cells. 2501669 1 28 gly glycoprotein 198:209 arg1 T4-binding globulin T4-binding globulin OGER T4-binding globulin P05543 T4-binding globulin (TBG), a 54-kilodalton glycoprotein, is the major thyroid hormone transport protein in man. 14670950 4 21 gly glycoforms 720:729 arg1 MT1-MMP glycoforms MT1-MMP glycoforms PUBTATOR MT1-MMP 4323 MT1-MMP glycoforms were detected in human cancer cell lines, suggesting that MT1-MMP activity may be regulated by differential glycosylation in vivo. 27966990 0 44 gly N-Glycosylation 14:28 arg1 Endothelial Cell Receptor Tyrosine Kinase VEGFR-2 Endothelial Cell Receptor Tyrosine Kinase VEGFR-2 OGER VEGFR-2 P35968 Site-Specific N-Glycosylation of Endothelial Cell Receptor Tyrosine Kinase VEGFR-2. 10751639 6 17 gly oligosaccharides 1275:1290 arg1 SHBG SHBG oligosaccharides PUBTATOR SHBG 6462 Approximately 9% of the biantennary complex oligosaccharides on SHBG of control men and none of those on SHBG from alcoholic men were fucosylated on the chitobiose core, as determined by chromatography on Lenn culinaris lectin. 10751639 6 29 gly SHBG 1295:1298 arg1 the biantennary complex oligosaccharides SHBG the biantennary complex oligosaccharides PUBTATOR SHBG 6462 Approximately 9% of the biantennary complex oligosaccharides on SHBG of control men and none of those on SHBG from alcoholic men were fucosylated on the chitobiose core, as determined by chromatography on Lenn culinaris lectin. 8786130 1 51 gly glycoprotein 186:197 arg1 Palmitoyl-protein thioesterase Palmitoyl-protein thioesterase PUBTATOR Palmitoyl-protein thioesterase 5538 Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. 1703212 10 46 gly glycosylation 1685:1697 arg1 gp 120 gp 120 PUBTATOR gp 120 155971 Changes in the glycosylation pattern of gp 120 may therefore contribute to the control of HIV-1 spread within its host. 16959765 8 9 gly N-glycosylation 1815:1829 arg1 the alpha5 subunit the alpha5 subunit OGER subunit 281873 Taken together, this study reveals for the first time that the N-glycosylation on the beta-propeller domain of the alpha5 subunit is essential for heterodimerization and biological functions of alpha5beta1 integrin and might also be useful for studies of the molecular structure. 10715125 0 45 gly thrombopoietin 21:34 arg1 The glycan domain thrombopoietin The glycan domain PUBTATOR thrombopoietin 7066 The glycan domain of thrombopoietin enhances its secretion. 14985108 3 35 gly glycosylated 423:434 arg1 glycosylated ONC ONC (gONC Cterm ONC (gONC We employed the Pichia pastoris expression system to produce recombinant glycosylated ONC (gONC) protein. 1935803 0 76 gly receptors 37:45 arg1 Glucose regulation growth hormone receptors Glucose regulation OGER growth hormone receptors P01244 Glucose regulation of growth hormone receptors in primary cultured rat hepatocytes. 14715137 6 45 gly N-glycosylation 985:999 arg1 synaptotagmin 1 synaptotagmin 1 PUBTATOR synaptotagmin 1 6857 Our data suggest that the intravesicular N-glycosylation site of synaptotagmin 1 collaborates with its cytoplasmic C(2) domains in directing synaptotagmin 1 to synaptic vesicles via a novel N-glycosylation-dependent mechanism. 17967194 9 114 gly glycosylated 1563:1574 arg1 a biologically active glycosylated r-alpha1-PI a biologically active glycosylated r-alpha1-PI Cterm r-alpha1-PI SDS-PAGE, Western blot, ELISA, and alpha1-PI activity assays enabled us to select the transformant(s) secreting a biologically active glycosylated r-alpha1-PI with yields of up to 12 mg/L. 11083795 5 49 gly CaMp65 768:773 arg1 all previously characterized tryptic fragments CaMp65 all previously characterized tryptic fragments Cterm CaMp65 Its deduced amino acid sequence showed regions of identity with all previously characterized tryptic fragments of CaMp65, as well as with the corresponding regions of ScMp65. 24716439 0 42 gly transferrin 46:56 arg1 a determinant transferrin a determinant PUBTATOR transferrin 7018 The glycation site specificity of human serum transferrin is a determinant for transferrin's functional impairment under elevated glycaemic conditions. 3543499 4 70 gly POMC 463:466 arg1 all the biosynthetic derivatives POMC all the biosynthetic derivatives PUBTATOR POMC 24664 A comprehensive study of all the biosynthetic derivatives of POMC in the neurointermediate lobe of the rat and mouse pituitary was undertaken. 7964612 13 90 gly unglycosylated 2109:2122 arg1 unglycosylated M protein unglycosylated M protein OGER M protein P54296 These findings suggest that (i) the M protein was probably translated and co-translocated into the ER and at least one site was glycosylated before leaving the ER resulting in no secretion of unglycosylated M protein, and (ii) the M protein had two secretion pathways, one through the conventional pathway and the other probably directly through the ER. 8163463 4 0 gly glycosylated 737:748 arg1 kainate receptor subunits kainate receptor subunits OGER subunits 2898 In this study we have shown that both AMPA receptor subunits (GluR1-4) and kainate receptor subunits (GluR6/7) are glycosylated in adult rat brain; however, the kainate receptor subunits are glycosylated to a greater extent. 8163463 4 0 gly glycosylated 737:748 arg1 GluR1-4 GluR1-4 PUBTATOR GluR1 50592 In this study we have shown that both AMPA receptor subunits (GluR1-4) and kainate receptor subunits (GluR6/7) are glycosylated in adult rat brain; however, the kainate receptor subunits are glycosylated to a greater extent. 8163463 4 0 gly glycosylated 737:748 arg1 GluR6/7 GluR6/7 PUBTATOR GluR6 54257 In this study we have shown that both AMPA receptor subunits (GluR1-4) and kainate receptor subunits (GluR6/7) are glycosylated in adult rat brain; however, the kainate receptor subunits are glycosylated to a greater extent. 8163463 4 0 gly glycosylated 737:748 arg1 both AMPA receptor subunits both AMPA receptor subunits OGER AMPA receptor subunits P19493 In this study we have shown that both AMPA receptor subunits (GluR1-4) and kainate receptor subunits (GluR6/7) are glycosylated in adult rat brain; however, the kainate receptor subunits are glycosylated to a greater extent. 8163463 4 76 gly glycosylated 813:824 arg1 the kainate receptor subunits the kainate receptor subunits OGER subunits 2898 In this study we have shown that both AMPA receptor subunits (GluR1-4) and kainate receptor subunits (GluR6/7) are glycosylated in adult rat brain; however, the kainate receptor subunits are glycosylated to a greater extent. 22344255 4 36 gly N-glycosylation 554:568 arg1 neural cadherin neural cadherin OGER neural cadherin P19022 Mutations that ablate N-glycosylation at three sites on the extracellular domains 2 and 3 of neural cadherin alter this kinetic fingerprint. 18642129 6 30 gly glycoprotein 912:923 arg1 FVII FVII OGER FVII P08709 A MALDI-MS analysis of the native protein indicated that FVII is a 50.1 kDa glycoprotein modified on two sites by diantennary, disialylated non-fucosylated (A2S2) glycans. 27489265 1 32 gly glycoprotein 144:155 arg1 Env Env PUBTATOR Env 155971 The envelope glycoprotein (Env) is the major target for HIV-1 broadly neutralizing antibodies (bNAbs). 27489265 1 32 gly glycoprotein 144:155 arg1 The envelope glycoprotein The envelope glycoprotein PUBTATOR envelope glycoprotein 155971 The envelope glycoprotein (Env) is the major target for HIV-1 broadly neutralizing antibodies (bNAbs). 2226797 0 48 gly variant 64:70 arg1 Carbohydrate structures tissue plasminogen activator variant Carbohydrate structures PUBTATOR tissue plasminogen activator variant 100128998 Carbohydrate structures of a human tissue plasminogen activator variant expressed in recombinant Chinese hamster ovary cells. 27716795 1 23 gly glycosylation 123:135 arg1 gp120 gp120 PUBTATOR gp120 155971 Heavy glycosylation of the envelope (Env) surface subunit, gp120, is a key adaptation of HIV-1; however, the precise effects of glycosylation on the folding, conformation and dynamics of this protein are poorly understood. 15982476 4 64 gly fAGP 646:649 arg1 The glycan moiety AGP The glycan moiety PUBTATOR AGP 100144393 The glycan moiety of fAGP was investigated by means of the binding of its oligosaccharides residues with specific lectins. 11063734 5 13 gly glycosylation 683:695 arg1 proSP-B proSP-B PUBTATOR SP-B 6439 Of the two SP-B polymorphisms genotyped, the Ile131Thr variation affects a putative N-terminal N:-linked glycosylation site of proSP-B and the length variation of intron 4 has previously been suggested to associate with RDS. 29867943 9 8 gly sialylated 1640:1649 arg1 antigen-specific sialylated human IgG4 antigen-specific sialylated human IgG4 OGER IgG4 P01861 Distinct IgG subclass distributions and functionally opposite IgG Fc glycosylation patterns might explain different outcomes of IgG-mediated immune responses and provide new therapeutic options through the induction, enrichment, or application of antigen-specific sialylated human IgG4 to prevent complement and FcγR activation as well. 27384988 7 41 gly glycosylation 672:684 arg1 rpS3 rpS3 PUBTATOR rpS3 6188 N-linked glycosylation of rpS3 was confirmed as necessary for rpS3 secretion into culture media via the ER-Golgi dependent pathway. 18952059 6 29 gly glycosylation 755:767 arg1 IZUMO IZUMO PUBTATOR IZUMO 73456 These data suggest that glycosylation is not essential for the function of IZUMO, but has a role in protecting it from fragmentation in cauda epididymis. 19818407 6 42 gly monoglycosylated 1016:1031 arg1 bCD38 bCD38 PUBTATOR CD38 327677 We found by site-directed mutagenesis and mass spectrometry that bCD38 was a monoglycosylated protein at Asn-201. 15047148 6 17 gly hypoglycosylated 824:839 arg1 hypoglycosylated wildtype CD28 hypoglycosylated wildtype CD28 PUBTATOR CD28 940 Unlike hypoglycosylated wildtype CD28, hypoglycosylation of CD28i did not alter CD28i functions. 15047148 6 32 gly hypoglycosylation 856:872 arg1 CD28i CD28i PUBTATOR CD28i 940 Unlike hypoglycosylated wildtype CD28, hypoglycosylation of CD28i did not alter CD28i functions. 20943674 9 68 gly unglycosylated 1472:1485 arg1 rhLF rhLF OGER rhLF P02788 The third putative glycosylation site, at Asn624, is unglycosylated in both hLF and rhLF. 20943674 9 68 gly unglycosylated 1472:1485 arg1 hLF hLF PUBTATOR hLF 3131 The third putative glycosylation site, at Asn624, is unglycosylated in both hLF and rhLF. 15183061 4 12 gly N-glycosylated 563:576 arg1 non-N-glycosylated or N-glycosylated CXCR4 non-N-glycosylated or N-glycosylated CXCR4 PUBTATOR N-glycosylated CXCR4 7852 Similar results were observed in binding studies using non-N-glycosylated or N-glycosylated CXCR4 expressed on cells. 15183061 4 69 gly non-N-glycosylated 541:558 arg1 non-N-glycosylated or N-glycosylated CXCR4 non-N-glycosylated or N-glycosylated CXCR4 PUBTATOR N-glycosylated CXCR4 7852 Similar results were observed in binding studies using non-N-glycosylated or N-glycosylated CXCR4 expressed on cells. 20208072 5 30 part_of GRP78 1026:1030 arg1 the putative O-linked glycosylation site Thr(648) GRP78 the putative O-linked glycosylation site Thr(648) PUBTATOR SpecificSite GRP78 3309 site Thr(648) Moreover, deletion of the C-terminal ER retention motif in GRP78 alters its cell surface presentation in a dose-dependent manner; however, mutation of the putative O-linked glycosylation site Thr(648) of human GRP78 is without effect. 14670950 8 0 gly glycosylation 1702:1714 arg1 MT1-MMP MT1-MMP PUBTATOR MT1-MMP 4323 These data provide evidence for an additional mechanism for post-translational control of MT1-MMP activity and suggest that glycosylation of MT1-MMP may regulate its substrate targeting. 10828967 4 14 gly sites 684:688 arg1 the PTH/PTHrP receptor PTHrP receptor sites PUBTATOR PTHrP receptor 24695 The results revealed that all four potential N-glycosylation sites in the PTH/PTHrP receptor are glycosylated. 8639667 6 13 gly glycosylation 1089:1101 arg1 ICAM-1 ICAM-1 PUBTATOR ICAM-1 3383 It has been proposed that the extent of N-linked glycosylation at Asn-240 and Asn-269 in the third domain of ICAM-1 may regulate the binding avidity of ICAM-1 to Mac-1 [Diamond, M. S., Staunton, D. E., Marlin, S. D., & Springer, T. A. (1991) Cell 65, 961-971]. 12901863 3 10 gly glycosylated 455:466 arg1 The human ABCC6 The human ABCC6 PUBTATOR ABCC6 368 The human ABCC6 in MDCKII cells was found to be glycosylated, in contrast to the underglycosylated form of the protein, as expressed in Sf9 cells. 16401092 8 51 gly core 1338:1341 arg1 huZP3 huZP3 core Cterm huZP3 7784 However, in huZP3 derived from rescue mice, the O-glycans associated with Thr-156 (analogous to Thr-155 in mZP3) are exclusively core 1 and related Tn sequences, whereas core 2 O-glycans predominate at the other conserved site. 15322230 6 41 gly P-glycoprotein 973:986 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Treatment of multidrug-resistant cells with 12-O-tetradecanoylphorbol-13-acetate, a phorbol ester that increases the phosphorylation of P-glycoprotein through activation of protein kinase C, or substituting phosphorylation sites of P-glycoprotein by nonphosphorylatable residues did not affect the ubiquitination of the transporter. 15322230 6 58 gly P-glycoprotein 1069:1082 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Treatment of multidrug-resistant cells with 12-O-tetradecanoylphorbol-13-acetate, a phorbol ester that increases the phosphorylation of P-glycoprotein through activation of protein kinase C, or substituting phosphorylation sites of P-glycoprotein by nonphosphorylatable residues did not affect the ubiquitination of the transporter. 9365923 3 37 gly glycosylated 436:447 arg1 human alpha-lactalbumin human alpha-lactalbumin PUBTATOR alpha-lactalbumin 3906 We have found that human alpha-lactalbumin is glycosylated and the glycosylation site has been determined by protein sequencing and mass spectrometry. 11467948 7 41 gly resialylated 1202:1213 arg1 TNFR-IgG TNFR-IgG PUBTATOR TNFR 7132 To increase the level of terminal sialylation, we regalactosylated and/or resialylated TNFR-IgG using beta-1,4-galactosyltransferase (beta1,4GT) and/or alpha-2,3-sialyltransferase (alpha2,3ST). 12087059 3 5 gly nonglycosylated 663:677 arg1 N30D-Edg-1 N30D-Edg-1 PUBTATOR Edg-1 1901 We found for the first time that Edg-1 is glycosylated in its amino-terminal extracellular portion, and further identified the specific glycosylation site as asparagine 30 by creating a nonglycosylated mutant of Edg-1 (N30D-Edg-1) and transfecting it into cell lines. 12087059 3 13 gly glycosylated 519:530 arg1 Edg-1 Edg-1 PUBTATOR Edg-1 1901 We found for the first time that Edg-1 is glycosylated in its amino-terminal extracellular portion, and further identified the specific glycosylation site as asparagine 30 by creating a nonglycosylated mutant of Edg-1 (N30D-Edg-1) and transfecting it into cell lines. 1371281 5 31 gly contain 678:684 arg1 cytokeratin 8 and 18 AND single O-linked N-acetylglucosamine residues cytokeratin 8 and 18 single O-linked N-acetylglucosamine residues PUBTATOR cytokeratin 8 3856 beta-Elimination of the [3H]galactose- labeled CK8/18 generated the disaccharide N-acetyllactosaminitol, indicating that cytokeratin 8 and 18 contain single O-linked N-acetylglucosamine residues. 16253890 0 43 gly gp120 25:29 arg1 the glycans gp120 the glycans PUBTATOR gp120 3700 Targeting the glycans of gp120: a novel approach aimed at the Achilles heel of HIV. 8639667 1 101 gly glycosylation 297:309 arg1 recombinant human ICAM-1des454-532 [tICAM tICAM(453 Cterm tICAM(453 Sialylated oligosaccharide structures were determined by the technique of electrospray ionization mass spectroscopy at seven of eight N-linked glycosylation sites of recombinant human ICAM-1des454-532 [tICAM(453)] purified from the tissue culture fluid of Chinese hamster ovary, human embryonic kidney, and mouse myeloma cell lines. 18434322 0 38 gly AMACO 116:120 arg1 the first epidermal growth factor repeat AMACO the first epidermal growth factor repeat PUBTATOR AMACO 340706 O-glucosylation and O-fucosylation occur together in close proximity on the first epidermal growth factor repeat of AMACO (VWA2 protein). 8096511 10 1 gly P-glycoprotein 1470:1483 arg1 P-glycoprotein structure P-glycoprotein structure PUBTATOR P-glycoprotein 5243 Our findings support the current model for P-glycoprotein structure. 28486782 2 51 gly glycosylated 223:234 arg1 EGFR EGFR PUBTATOR EGFR 1956 The extracellular domain of EGFR is known to be heavily glycosylated. 10940860 3 23 gly occupancy 472:480 arg1 t-PA t-PA OGER t-PA P00750 In this report, the site occupancy of t-PA is shown to increase gradually over the course of batch and fed-batch CHO cultures. 12626422 4 64 gly glycosylated 757:768 arg1 alpha1-antitrypsin alpha1-antitrypsin PUBTATOR alpha1-antitrypsin 5265 Human plasma alpha1-antitrypsin is normally fully glycosylated at three asparagine residues (46, 83, and 247), but un-, mono-, di-, and fully glycosylated forms of alpha1-antitrypsin were detected by 2D PAGE in the plasma from patients with CDG-I. 12626422 4 70 gly glycosylated 665:676 arg1 Human plasma alpha1-antitrypsin Human plasma alpha1-antitrypsin PUBTATOR alpha1-antitrypsin 5265 Human plasma alpha1-antitrypsin is normally fully glycosylated at three asparagine residues (46, 83, and 247), but un-, mono-, di-, and fully glycosylated forms of alpha1-antitrypsin were detected by 2D PAGE in the plasma from patients with CDG-I. 12498206 4 42 gly alpha-2HS-glycoprotein 865:886 arg1 human alpha-2HS-glycoprotein isoforms human alpha-2HS-glycoprotein isoforms PUBTATOR alpha-2HS-glycoprotein isoforms 197 The method was used to demonstrate that the difference between human alpha-2HS-glycoprotein isoforms separated by 2D-gel electrophoresis was partially due to sialylation of both O-linked and N-linked oligosaccharides. 1710279 6 89 gly glycoproteins 1052:1064 arg1 P0 P0 Cterm P0 Comparison of the corresponding myelin basic proteins (MBP) and P0 glycoproteins (P0) for rodent and shark showed that the conserved residues included most of the amino acids which were predicted to form the alpha or beta conformations, while the altered residues were mainly in the hydrophilic and turn or coil regions. 10395084 2 81 gly glycosylated 505:516 arg1 native human glycosylated uPA native human glycosylated uPA PUBTATOR uPA 5328 The isoforms tested included native human glycosylated uPA, and two recombinant uPA forms, namely a recombinant uPA with wild type structure (r-uPA), and a uPA-mutant in which the first 24 N-terminal amino acid residues of the receptor binding domain were replaced by 13 foreign amino acid residues (r-uPAmut). 27377235 5 12 gly Glycosylation 887:899 arg1 Kv1.2 Kv1.2 Cterm Kv1.2 Glycosylation of Kv1.2 is important with respect to facilitating trafficking to the cell membrane and enhancing the stability of channels that have reached the cell membrane. 10362843 3 88 gly glycosylation 393:405 arg1 N22Q-V2R N22Q-V2R PUBTATOR V2R 554 Mutagenesis of asparagine 22 to glutamine abolished N-linked glycosylation of the V2 receptor (N22Q-V2R), without altering its function or level of expression. 9638944 8 36 gly glycoprotein 1625:1636 arg1 alpha2-HS glycoprotein alpha2-HS glycoprotein PUBTATOR alpha2-HS glycoprotein 197 These approaches have been proved on 1-D PAGE electroblotted bovine fetuin and human glycophorin A and then used to analyze two abundant proteins which separate as glycoforms on 2-D PAGE preparative narrow range (pH 4.5-5.5) blots of human plasma: alpha2-HS glycoprotein (human fetuin) and alpha1-antitrypsin (alpha1-protease inhibitor). 8286855 1 46 gly sialoglycoprotein 144:160 arg1 glycophorin A glycophorin A PUBTATOR glycophorin A 2993 The human red blood cell sialoglycoprotein, glycophorin A (GpA), contains a 'mucin-like' extensively O-glycosylated extracellular domain which carries the MN blood group antigens. 24799124 1 18 gly ceruloplasmin 212:224 arg1 core-fucosylation ceruloplasmin core-fucosylation PUBTATOR ceruloplasmin 1356 A mass spectrometry-based methodology has been developed to study changes in core-fucosylation of serum ceruloplasmin that are site-specific between cirrhosis and hepatocellular carcinoma (HCC). 24799124 1 48 gly core-fucosylation 185:201 arg1 serum ceruloplasmin serum ceruloplasmin PUBTATOR ceruloplasmin 1356 A mass spectrometry-based methodology has been developed to study changes in core-fucosylation of serum ceruloplasmin that are site-specific between cirrhosis and hepatocellular carcinoma (HCC). 10603362 9 1 gly P120 1759:1762 arg1 the repeat regions P120 the repeat regions PUBTATOR P120 1500 The presence of only one site for N-linked (Asn-Xaa-Ser/Thr) glycosylation, a lack of effect of N-glycosidase F, the presence of 70 and 126 Ser/Thr glycosylation sites in the repeat regions of P120 and P140, respectively, and a high molar ratio of carbohydrate to protein suggest that the glycans may be O linked. 10603362 9 15 gly P140 1768:1771 arg1 the repeat regions P140 the repeat regions PUBTATOR P140 80725 The presence of only one site for N-linked (Asn-Xaa-Ser/Thr) glycosylation, a lack of effect of N-glycosidase F, the presence of 70 and 126 Ser/Thr glycosylation sites in the repeat regions of P120 and P140, respectively, and a high molar ratio of carbohydrate to protein suggest that the glycans may be O linked. 3360214 5 0 gly glycosylated 840:851 arg1 nonenzymatically glycosylated laminin nonenzymatically glycosylated laminin OGER laminin An analysis of the stoichiometry of [3H]heparin binding to control and nonenzymatically glycosylated laminin at saturating levels of heparin was performed. 15205186 6 23 part_of has 952:954 arg1 The qAQP2 AND putative N-glycosylation (asparagine-124) and phosphorylation sites The qAQP2 putative N-glycosylation (asparagine-124) and phosphorylation sites PUBTATOR SpecificSite AQP2 378655 asparagine-124 The qAQP2 has six transmembrane domains, two asparagine-proline-alanine (NPA) sequences, and putative N-glycosylation (asparagine-124) and phosphorylation sites (serine-257) for cAMP-dependent protein kinase. 15205186 6 23 part_of has 952:954 arg1 The qAQP2 AND serine-257 The qAQP2 serine-257 PUBTATOR SpecificSite AQP2 378655 serine-257 The qAQP2 has six transmembrane domains, two asparagine-proline-alanine (NPA) sequences, and putative N-glycosylation (asparagine-124) and phosphorylation sites (serine-257) for cAMP-dependent protein kinase. 18636497 0 45 gly sialylation 26:36 arg1 recombinant human interferon-gamma recombinant human interferon-gamma PUBTATOR interferon-gamma 3458 Site- and branch-specific sialylation of recombinant human interferon-gamma in Chinese hamster ovary cell culture. 18636497 0 92 gly interferon-gamma 59:74 arg1 branch-specific sialylation interferon-gamma branch-specific sialylation PUBTATOR interferon-gamma 3458 Site- and branch-specific sialylation of recombinant human interferon-gamma in Chinese hamster ovary cell culture. 7658166 13 8 gly non-glycosylated 2207:2222 arg1 the non-glycosylated LPL the non-glycosylated LPL PUBTATOR LPL 4023 Our findings demonstrate that glycosylation of Asn43 of human lipoprotein lipase in the endoplasmic reticulum is essential for its efflux from this compartment and that the retention of the non-glycosylated LPL induces morphological changes in the ER that could also affect its ability to modify the transport of other proteins. 17609437 11 57 gly monoglycosylated 1865:1880 arg1 monoglycosylated GPHalpha monoglycosylated GPHalpha PUBTATOR GPHalpha 1081 The studies demonstrate the formation, in vivo dynamics of GPHalpha alpha homodimers, and the pathways of the cellular metabolism of variants of GPHalpha, monoglycosylated GPHalpha and large free GPHalpha. 10593893 3 13 gly detected 765:772 arg1 beta-trace protein AND Lewis X ratios beta-trace protein Lewis X ratios PUBTATOR beta-trace protein 5730 The sialyl Lewis X:Lewis X ratios detected in beta-trace protein indicate that the CTS regions of the early acting GlcNAc-transferases I (GnT-I) and III (GnT-III) specify backward targeting of the FT6 catalytic domain, whereas the CTS region of the late acting human alpha1,3-fucosyltransferase VII (FT7) causes forward targeting of the FT6 in vivo activity in the biosynthetic glycosylation pathway. 1413513 6 49 gly nonglycosylated 1533:1547 arg1 nonglycosylated SHo nonglycosylated SHo OGER SHo Q5BIV9 In addition to SHp, nonglycosylated SHo was selected by Dsl affinity, indicating that SHp and SHo may associate to form complexes within infected cells and virus particles. 25336660 11 47 gly N-glycosylation 1582:1596 arg1 ADAM8 ADAM8 PUBTATOR ADAM8 101 Thus, N-glycosylation is essential for processing, localization, stability, and activity of ADAM8. 25389233 9 74 gly IgG 1438:1440 arg1 the glycan IgG the glycan Cterm IgG We studied associations of the glycan of complexed IgG and disease activity according to the physician's global assessment of disease activity and the systemic lupus erythematosus disease activity index 2000 documented at the moment of blood taking. 1692830 1 60 gly glycoprotein 110:121 arg1 Endoglin Endoglin PUBTATOR Endoglin 2022 Endoglin is a major glycoprotein of human vascular endothelium. 27480168 2 9 gly glycosylated 387:398 arg1 hENT1 hENT1 OGER hENT1 Q99808 Previous work, and in silico prediction, suggest that hENT1 is glycosylated at Asn(48) in the first extracellular loop of the protein and that glycosylation plays a role in correct localization and function of hENT1. 12354382 2 4 gly ligand 328:333 arg1 a novel carbohydrate modification P selectin glycoprotein ligand 1 a novel carbohydrate modification PUBTATOR P selectin glycoprotein ligand 1 6404 Here we identify the M-DC8 structure as 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1). 12354382 2 40 gly glycoprotein 315:326 arg1 the P selectin glycoprotein ligand 1 the P selectin glycoprotein ligand 1 PUBTATOR P selectin glycoprotein ligand 1 6404 Here we identify the M-DC8 structure as 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1). 12354382 2 44 gly modification 284:295 arg1 PSGL-1 AND a novel carbohydrate modification PSGL-1 a novel carbohydrate modification PUBTATOR PSGL-1 6404 Here we identify the M-DC8 structure as 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1). 12354382 2 44 gly modification 284:295 arg1 the P selectin glycoprotein ligand 1 AND a novel carbohydrate modification the P selectin glycoprotein ligand 1 a novel carbohydrate modification PUBTATOR P selectin glycoprotein ligand 1 6404 Here we identify the M-DC8 structure as 6-sulfo LacNAc, a novel carbohydrate modification of the P selectin glycoprotein ligand 1 (PSGL-1). 24473128 2 55 gly contains 228:235 arg1 The GP1 subunit AND the glycan cap The GP1 subunit the glycan cap PUBTATOR GP1 subunit 14904 The GP1 subunit contains two heavily glycosylated domains, the glycan cap and the mucin-like domain (MLD). 11780780 1 30 gly prolactin 108:116 arg1 subsequent proper carbohydrate characterization prolactin subsequent proper carbohydrate characterization OGER prolactin P01237 Isolation of glycosylated 26 kDa rat prolactin and subsequent proper carbohydrate characterization has so far not been reported. 11780780 1 76 gly glycosylated 84:95 arg1 glycosylated 26 kDa rat prolactin glycosylated 26 kDa rat prolactin OGER prolactin P01237 Isolation of glycosylated 26 kDa rat prolactin and subsequent proper carbohydrate characterization has so far not been reported. 27760464 4 14 gly glycoprotein 389:400 arg1 AGP AGP Cterm AGP Using alpha-1-acid glycoprotein (AGP) as a model N-glycoprotein, we identified its tryptic N-glycopeptides and examined the data reproducibility in seven laboratories running different LC-MS/MS platforms. 8985126 0 18 gly sialoglycoprotein 109:125 arg1 the human MG160 the human MG160 PUBTATOR MG160 2734 Cloning and sequence analysis of the human MG160, a fibroblast growth factor and E-selectin binding membrane sialoglycoprotein of the Golgi apparatus. 12096136 4 2 gly F-spondin 697:705 arg1 the thrombospondin type 1 repeats F-spondin the thrombospondin type 1 repeats PUBTATOR F-spondin 10418 Their analysis by a combined mass spectrometric approach is illustrated with peptides from the thrombospondin type 1 repeats (TSRs) of the recombinant axonal guidance protein F-spondin. 1711570 2 40 gly glycoprotein 482:493 arg1 MCP MCP PUBTATOR MCP 4179 The structure of a previously reported cDNA clone indicated that MCP was a type 1 membrane glycoprotein and a member of the regulators of complement activation gene/protein cluster. 10353820 2 42 gly O-fucosylation 315:328 arg1 FVII EGF-1 FVII EGF-1 OGER structure of FVII EGF-1 P08709 We report here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, its Ca2+-binding affinity, and its interaction with tissue factor (TF). 26467158 3 16 gly modified 418:425 arg1 BACE1 AND GlcNAc BACE1 GlcNAc PUBTATOR BACE1 23821 We have recently found that BACE1 is modified with bisecting N-acetylglucosamine (GlcNAc) by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene) and that GnT-III deficiency reduces Aβ-plaque formation in the brain by accelerating lysosomal degradation of BACE1. 26467158 3 16 gly modified 418:425 arg3 BACE1 AND bisecting N-acetylglucosamine BACE1 bisecting N-acetylglucosamine PUBTATOR BACE1 23821 We have recently found that BACE1 is modified with bisecting N-acetylglucosamine (GlcNAc) by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene) and that GnT-III deficiency reduces Aβ-plaque formation in the brain by accelerating lysosomal degradation of BACE1. 29626154 3 50 gly O-glycosylation 507:521 arg1 OPN OPN PUBTATOR OPN 6696 However, the role of O-glycosylation in cell adhesion activity and phosphorylation of OPN remains to be clarified. 29898396 1 0 gly glycoprotein 185:196 arg1 Env Env PUBTATOR Env 100616444 Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. 29898396 1 0 gly glycoprotein 185:196 arg1 the HIV envelope glycoprotein the HIV envelope glycoprotein PUBTATOR HIV envelope glycoprotein 100616444 Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. 23187000 7 23 gly glycosylation 1330:1342 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 Cancer cells appear to utilize these bilateral aspects of TIMP-1 for cancer progression; an elevated TIMP-1 level exerts to cancer development via MMP-independent pathway during the early phase of tumor formation, whereas it is the aberrant glycosylation of TIMP-1 that overcome the high anti-proteolytic burden. 2965020 5 44 part_of HP1 1038:1040 arg1 residues 42-102 HP1 residues 42-102 PUBTATOR SpecificSite HP1 23468 residues 42-102 Comparison of the cDNA sequence of HP1 with that of human interleukin 6 disclosed a homology of 65% at the DNA level and of 42% at the protein level with a maximum of 57% for the segment spanning residues 42-102 of mature HP1. 9832151 7 37 gly unglycosylated 1178:1191 arg1 rVMAT1 form of rVMAT1 PUBTATOR form of rVMAT1 25693 It is interesting that in contrast to plasma-membrane neurotransmitter transporters, the unglycosylated form of rVMAT1 distributed in the cell as the wild-type protein. 10763868 2 71 gly AGP 424:426 arg1 a sugar moiety AGP a sugar moiety Cterm AGP Removal of a sugar moiety of AGP by treatment with N-glycosidase was confirmed by high-performance capillary electrophoresis, reversed-phase HPLC and matrix-assisted laser desorption-time of flight (MALDI-TOF) mass spectrometry. 21044954 8 35 gly glycosylation 1431:1443 arg1 endothelial cell protein C receptor-dependent cytoprotective signaling endothelial cell protein C receptor OGER endothelial cell protein C receptor Q9UNN8 These data highlight the previously unidentified role of APC N-linked glycosylation in modulating endothelial cell protein C receptor-dependent cytoprotective signaling via PAR1. 21044954 8 35 gly glycosylation 1431:1443 arg1 endothelial cell protein C receptor-dependent cytoprotective signaling endothelial cell protein C receptor OGER endothelial cell protein C receptor Q9UNN8 These data highlight the previously unidentified role of APC N-linked glycosylation in modulating endothelial cell protein C receptor-dependent cytoprotective signaling via PAR1. 21044954 8 35 gly glycosylation 1431:1443 arg1 endothelial cell protein C receptor-dependent cytoprotective signaling endothelial cell protein C receptor OGER endothelial cell protein C receptor Q9UNN8 These data highlight the previously unidentified role of APC N-linked glycosylation in modulating endothelial cell protein C receptor-dependent cytoprotective signaling via PAR1. 29717117 7 44 gly N-glycosylation 1043:1057 arg1 mTRAIL-R mTRAIL-R PUBTATOR mTRAIL 22035 Instead, it relied on the inhibition of N-glycosylation of the mouse TRAIL receptor (mTRAIL-R). 29717117 7 44 gly N-glycosylation 1043:1057 arg1 the mouse TRAIL receptor the mouse TRAIL receptor PUBTATOR TRAIL receptor 8743 Instead, it relied on the inhibition of N-glycosylation of the mouse TRAIL receptor (mTRAIL-R). 11570856 0 75 gly nonglycosylated 62:76 arg1 recombinant nonglycosylated human serum transferrin recombinant nonglycosylated human serum transferrin PUBTATOR transferrin 101828182 Expression, purification, and characterization of recombinant nonglycosylated human serum transferrin containing a C-terminal hexahistidine tag. 10647817 4 19 part_of kinase 1348:1353 arg1 Ser295 casein kinase II Ser295 OGER AminoAcid casein kinase II Ser295 All the important features of PTA1 molecule are conserved among these Primates: (1) the ORF encoding 336 amino acid residues including signal sequence (18aa), extracellular region (232aa), transmembrane sequence (25aa) and cytoplasmic region (61aa); (2) two conserved pairs of Cys (Cys19 to Cys90 and Cys134 to Cys204) forming disulfide bonds stabilizing the two immunoglobulin superfamily V-like domains; (3) eight putative N-linked glycosylation sites (except gPTA1 with nine sites) and three O-linked glycosylation sites in extracellular region; and (4) predicated protein kinase C phosphorylation sites (Thr275 and Ser311), casein kinase II sites (Ser295 and The299) and the potential tyrosine phosphorylation site (Tyr304). 10647817 4 67 part_of casein 1341:1346 arg1 Ser295 casein kinase II Ser295 OGER AminoAcid casein kinase II Ser295 All the important features of PTA1 molecule are conserved among these Primates: (1) the ORF encoding 336 amino acid residues including signal sequence (18aa), extracellular region (232aa), transmembrane sequence (25aa) and cytoplasmic region (61aa); (2) two conserved pairs of Cys (Cys19 to Cys90 and Cys134 to Cys204) forming disulfide bonds stabilizing the two immunoglobulin superfamily V-like domains; (3) eight putative N-linked glycosylation sites (except gPTA1 with nine sites) and three O-linked glycosylation sites in extracellular region; and (4) predicated protein kinase C phosphorylation sites (Thr275 and Ser311), casein kinase II sites (Ser295 and The299) and the potential tyrosine phosphorylation site (Tyr304). 10647817 4 73 part_of II 1355:1356 arg1 Ser295 casein kinase II Ser295 OGER AminoAcid casein kinase II Ser295 All the important features of PTA1 molecule are conserved among these Primates: (1) the ORF encoding 336 amino acid residues including signal sequence (18aa), extracellular region (232aa), transmembrane sequence (25aa) and cytoplasmic region (61aa); (2) two conserved pairs of Cys (Cys19 to Cys90 and Cys134 to Cys204) forming disulfide bonds stabilizing the two immunoglobulin superfamily V-like domains; (3) eight putative N-linked glycosylation sites (except gPTA1 with nine sites) and three O-linked glycosylation sites in extracellular region; and (4) predicated protein kinase C phosphorylation sites (Thr275 and Ser311), casein kinase II sites (Ser295 and The299) and the potential tyrosine phosphorylation site (Tyr304). 22123080 3 2 gly N-glycosylation 364:378 arg1 CD82 CD82 PUBTATOR CD82 3732 However, the N-glycosylation pattern of CD82 has not been described yet. 29559555 5 41 gly GalNAc-transferase 766:783 arg1 GalNAc-T11 GalNAc-transferase GalNAc-T11 Cterm GalNAc-transferase Moreover, we found that O-glycan modifications at these sites are selectively controlled by the GalNAc-transferase isoform, GalNAc-T11. 25495042 4 66 part_of contains 601:608 arg1 TARP γ-8 AND Asn53 TARP γ-8 sites, Asn53 and Asn56 PUBTATOR AminoAcid TARP γ-8 59283 sites, Asn53 and Asn56 We first showed that TARP γ-8 is an N-glycosylated protein, which contains two glycosylation sites, Asn53 and Asn56, and compared this with the glycosylation of TARP γ-2 and the AMPA receptor auxiliary protein CNIH-2 (cornichon homologue 2). 25495042 4 66 part_of contains 601:608 arg1 TARP γ-8 AND two glycosylation sites TARP γ-8 sites, Asn53 and Asn56 PUBTATOR AminoAcid TARP γ-8 59283 sites, Asn53 and Asn56 We first showed that TARP γ-8 is an N-glycosylated protein, which contains two glycosylation sites, Asn53 and Asn56, and compared this with the glycosylation of TARP γ-2 and the AMPA receptor auxiliary protein CNIH-2 (cornichon homologue 2). 25495042 4 66 part_of contains 601:608 arg1 TARP γ-8 AND two glycosylation sites TARP γ-8 sites, Asn53 and Asn56 PUBTATOR AminoAcid TARP γ-8 59283 sites, Asn53 and Asn56 We first showed that TARP γ-8 is an N-glycosylated protein, which contains two glycosylation sites, Asn53 and Asn56, and compared this with the glycosylation of TARP γ-2 and the AMPA receptor auxiliary protein CNIH-2 (cornichon homologue 2). 29339411 3 66 gly O-glycosylated 671:684 arg1 a diversely and heavily O-glycosylated flagellin C9LY14 a diversely and heavily O-glycosylated flagellin C9LY14 Cterm C9LY14 Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides. 29339411 3 82 gly carries 738:744 arg1 a diversely and heavily O-glycosylated flagellin C9LY14 AND hitherto undescribed rhamnose- a diversely and heavily O-glycosylated flagellin C9LY14 hitherto undescribed rhamnose- Cterm C9LY14 Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides. 29339411 3 82 gly carries 738:744 arg1 a diversely and heavily O-glycosylated flagellin C9LY14 AND N-acetylglucosamine linked O-glycans a diversely and heavily O-glycosylated flagellin C9LY14 N-acetylglucosamine linked O-glycans Cterm C9LY14 Here we provide the first report of a Selenomonas glycoprotein, showing that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N-acetylglucosamine linked O-glycans in the range from mono- to hexasaccharides. 20621206 0 31 gly glycosylation 4:16 arg1 myeloperoxidase myeloperoxidase PUBTATOR myeloperoxidase 4353 The glycosylation of myeloperoxidase. 16877748 4 45 gly carries 662:668 arg1 sICAM-1 AND complex-type N-glycans sICAM-1 complex-type N-glycans Cterm sICAM-1 3383 MIP-2 induction is glycosylation dependent, as it is strongly enhanced when sICAM-1 carries sialylated, complex-type N-glycans as synthesized by wild-type Chinese hamster ovary (CHO) cells. 18339697 2 62 gly O-glycosylated 304:317 arg1 proBNP proBNP PUBTATOR BNP 4879 Recently it has been shown that proBNP is O-glycosylated in human blood. 4083905 0 1 gly structures 19:28 arg1 murine IgM IgM structures OGER IgM P01872 Major carbohydrate structures at five glycosylation sites on murine IgM determined by high resolution 1H-NMR spectroscopy. 28746350 6 33 gly N-glycosylation 745:759 arg1 Vstm5 Vstm5 PUBTATOR Vstm5 69137 N-glycosylation at multiple sites affects differentially the function of Vstm5. 3367907 7 12 gly Unglycosylated 1323:1336 arg1 Unglycosylated NB Unglycosylated NB PUBTATOR Unglycosylated NB 4682 Unglycosylated NB, expressed either in influenza B virus-infected cells treated with tunicamycin or in cells expressing the NB mutant lacking both N-linked glycosylation sites, was expressed at the cell surface, indicating that NB does not require carbohydrate addition for transport. 27938679 1 23 gly glycoproteins 283:295 arg1 TM TM Cterm TM The bovine leukaemia virus (BLV) envelope protein (Env) is synthesized as a polyprotein precursor (gp72) proteolytically cleaved into the mature surface (SU) and transmembrane (TM) glycoproteins. 28167607 4 15 gly contains 491:498 arg1 Wild-type (WT) integrin α5 and N-glycosylation mutant S3-5 (sites 3 to 5) integrin α5 AND fewer N-glycans Wild-type (WT) integrin α5 and N-glycosylation mutant S3-5 (sites 3 to 5) integrin α5 fewer N-glycans PUBTATOR 5) integrin α5 3678 Wild-type (WT) integrin α5 and N-glycosylation mutant S3-5 (sites 3 to 5) integrin α5, which contains fewer N-glycans, were stably reconstituted in α5 knockout cancer cells. 17927214 2 17 gly CI-MPR 529:534 arg1 two high-affinity Man-6-P binding sites CI-MPR two high-affinity Man-6-P binding sites PUBTATOR CI-MPR 3482 Previous studies have mapped two high-affinity Man-6-P binding sites of the CI-MPR to domains 1-3 and 9 and one low-affinity site to domain 5 within its 15-domain extracytoplasmic region. 26328495 3 49 gly O-glycosylated 447:460 arg1 POMGNT1 POMGNT1 PUBTATOR POMGNT1 55624 To determine whether POMGNT1 is O-glycosylated, we prepared recombinant human POMGNT1 from HEK293T cells. 1508225 10 71 gly ABP 1515:1517 arg1 All three mutant forms ABP All three mutant forms PUBTATOR ABP 24775 All three mutant forms of ABP were secreted by the COS cells. 8995188 4 6 gly Zn-alpha2-glycoprotein 508:529 arg1 Zn-alpha2-glycoprotein molecules Zn-alpha2-glycoprotein molecules PUBTATOR Zn-alpha2-glycoprotein 563 Two members of the family have been shown to be functional and share domains with members of the supergene family including HLA class I, FcRn, and Zn-alpha2-glycoprotein molecules. 7915183 9 17 gly deglycosylated 1140:1153 arg1 deglycosylated human CD2 deglycosylated human CD2 PUBTATOR CD2 914 The model provides an explanation for the observed instability of deglycosylated human CD2, and allows residues that are important for CD58 binding to be differentiated from those affecting conformational stability via interactions with the glycan. 11927624 5 51 gly glycosylation 943:955 arg1 SHBG SHBG PUBTATOR SHBG 6462 A single-nucleotide polymorphism within the proband's maternally derived SHBG allele encodes a missense mutation, P156L, which allows for normal steroid ligand binding but causes abnormal glycosylation and inefficient secretion of SHBG. 7657720 6 41 gly monoglycosylated 844:859 arg1 Procathepsin D Procathepsin D Cterm Procathepsin D Procathepsin D monoglycosylated at N70 is readily distinguished from the endogenous protein in transfected human cells and thus provides an excellent substrate for studying lysosomal targeting in an homologous system. 9139799 1 10 gly sites 260:264 arg1 rLHR rLHR sites PUBTATOR rLHR 25477 Using two separate methods, we have determined that all six potential sites for N-linked glycosylation on the rat lutropin/choriogonadotropin receptor (rLHR) contain carbohydrates. 9139799 1 10 gly sites 260:264 arg1 the rat lutropin/choriogonadotropin receptor lutropin/choriogonadotropin receptor sites PUBTATOR lutropin/choriogonadotropin receptor 3973 Using two separate methods, we have determined that all six potential sites for N-linked glycosylation on the rat lutropin/choriogonadotropin receptor (rLHR) contain carbohydrates. 10716671 11 63 gly N-glycosylated 1166:1179 arg1 N-glycosylated TFF2 N-glycosylated TFF2 PUBTATOR N-glycosylated TFF2 7032 This was shown to be N-glycosylated TFF2 using the endoglycosidase, peptide-N-Gycosidase F. 10393537 2 26 gly receptor 401:408 arg1 all three putative N-linked glycosylation consensus sequences AT1 receptor all three putative N-linked glycosylation consensus sequences OGER AT1 receptor O00400 Using site-directed mutagenesis, we substituted both separately and simultaneously the asparagine residues in all three putative N-linked glycosylation consensus sequences (N-X-S/T) of AT1 receptor (positions 4, 176, and 188) with aspartic acid. 21173144 6 70 part_of TPC2 1114:1117 arg1 residues 240-254 TPC2 residues 240-254 PUBTATOR SpecificSite TPC2 219931 residues 240-254 Immunocytochemical analysis of selectively permeabilized cells using antipeptide antibodies confirmed that the C-terminal tails of recombinant TPCs are cytosolic and that residues 240-254 of TPC2 prior to putative pore 1 are luminal. 25285362 0 37 gly glycosylation 89:101 arg1 HIV-1 gp120 HIV-1 gp120 OGER gp120 Q14624 Mass spectrometry approach and ELISA reveal the effect of codon optimization on N-linked glycosylation of HIV-1 gp120. 14522983 2 46 gly sialoglycoprotein 368:384 arg1 Aggrus/gp44 Aggrus/gp44 PUBTATOR Aggrus 10630 We have previously characterized the platelet aggregation-inducing sialoglycoprotein (Aggrus/gp44) overexpressed on the surface of tumor cells. 1577776 0 77 gly glycoprotein 22:33 arg1 human glycoprotein Ib alpha human glycoprotein Ib alpha PUBTATOR glycoprotein Ib alpha 2811 Polymorphism of human glycoprotein Ib alpha results from a variable number of tandem repeats of a 13-amino acid sequence in the mucin-like macroglycopeptide region. 27668402 2 64 gly glycoprotein 315:326 arg1 the 90K glycoprotein the 90K glycoprotein PUBTATOR 90K glycoprotein 3959 Previously, we showed that β-catenin is downregulated by the 90K glycoprotein via ISGylation-dependent degradation. 870150 1 53 gly glycoprotein 274:285 arg1 alpha 1-acid glycoprotein alpha 1-acid glycoprotein PUBTATOR alpha 1-acid glycoprotein 24614 Ultrasonic extracts of rough and smooth endoplasmic reticulum fraction and Golgi fractions from rat liver were examined by immunoelectrophoresis using antiserum to alpha 1-acid glycoprotein. 17660514 7 51 gly contained 944:952 arg1 rat and human corin proteins AND little O-glycans rat and human corin proteins little O-glycans PUBTATOR corin proteins 10699 Glycosidase digestion showed that rat and human corin proteins contained substantial N-glycans but little O-glycans. 17660514 7 51 gly contained 944:952 arg1 rat and human corin proteins AND substantial N-glycans rat and human corin proteins substantial N-glycans PUBTATOR corin proteins 10699 Glycosidase digestion showed that rat and human corin proteins contained substantial N-glycans but little O-glycans. 22967898 5 34 gly N-glycosylation 571:585 arg1 cathepsin V cathepsin V PUBTATOR cathepsin V 1515 N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells. 22159084 12 65 gly deglycosylated 1521:1534 arg1 deglycosylated SLC26A3 deglycosylated SLC26A3 PUBTATOR SLC26A3 1811 While the mature glycosylated SLC26A3 showed little breakdown after treatment with trypsin, deglycosylated SLC26A3 exhibited increased susceptibility to trypsin, suggesting that the oligosaccharides protect SLC26A3 from tryptic digestion. 22159084 12 68 gly glycosylated 1446:1457 arg1 the mature glycosylated SLC26A3 the mature glycosylated SLC26A3 PUBTATOR SLC26A3 1811 While the mature glycosylated SLC26A3 showed little breakdown after treatment with trypsin, deglycosylated SLC26A3 exhibited increased susceptibility to trypsin, suggesting that the oligosaccharides protect SLC26A3 from tryptic digestion. 29880742 10 30 gly α-1,6-fucosylated 2001:2017 arg1 core α-1,6-fucosylated TGF-β receptor II core α-1,6-fucosylated TGF-β receptor II PUBTATOR α-1,6-fucosylated TGF-β receptor II 21813 To verify this result, the well-known transforming growth factor-β (TGF-β)/Smad2/3 pathway was selected, and core α-1,6-fucosylated TGF-β receptor II (TGFR-βII) was analysed semi-quantitatively with western blotting. 26415233 1 13 gly N-glycosylated 159:172 arg1 nascent immature N-glycosylated 110kDa TrkA nascent immature N-glycosylated 110kDa TrkA PUBTATOR TrkA 4914 In human SH-SY5Y neuroblastoma (NB) cells, nascent immature N-glycosylated 110kDa TrkA moves rapidly from the endoplasmic reticulum (ER) to the Golgi Network (GN), where it matures into the 140kDa receptor prior to being transported to the cell surface, creating GN and cell surface pools of inactive receptor maintained below the spontaneous activation threshold by a full compliment of inhibitory domains and endogenous PTPases. 8081814 1 1 gly glycoprotein 165:176 arg1 ZP3 ZP3 PUBTATOR ZP3 7784 The zona pellucida surrounding the mammalian oocyte contains a major glycoprotein species, ZP3, that serves as a cell- and species-specific receptor for spermatozoa. 10562497 0 70 part_of gp110 63:67 arg1 positions 836-839 EBV gp110 positions 836-839 PUBTATOR SpecificSite EBV gp110 11047 arginine residues at positions 836-839 Four consecutive arginine residues at positions 836-839 of EBV gp110 determine intracellular localization of gp110. 23603833 6 38 gly LDLR-A 868:873 arg1 critical determinants LDLR critical determinants OGER LDLR P01130 The two LDLR-A domains (aa 54-89 and 132-167) with the negatively charged acidic residues involved in Ca(2+) binding are critical determinants of ligand binding. 12359240 6 8 gly glycosylated 849:860 arg1 TMEM9 TMEM9 PUBTATOR TMEM9 252839 COS-1 cells transfected with a TMEM9 expression plasmid gave three bands of about 28, 31, and 33kDa representing glycosylated forms of TMEM9 with a protein backbone of about 26kDa. 2125204 10 59 gly non-glycosylated 1355:1370 arg1 non-glycosylated IFN-gamma non-glycosylated IFN-gamma OGER IFN-gamma P01579 When the cells were grown in batch culture in serum-free medium under pH and dissolved-oxygen control, the proportion of non-glycosylated IFN-gamma increased from 3 to 5% after 3 h, to 30% of the total IFN-gamma present after 195 h. 26088564 1 42 gly glycoprotein 128:139 arg1 AGP AGP Cterm AGP Human α1-acid glycoprotein (AGP) is an abundant human plasma glycoprotein that may be N-glycosylated at five positions. 8341708 9 40 gly glycosylation 1310:1322 arg1 a 62-kDa EPOR a 62-kDa EPOR PUBTATOR EPOR 13857 (iv) Enzymatic deglycosylation and dephosphorylation showed that hmm-EPOR apparently resulted from additional N-linked glycosylation of a 62-kDa EPOR. 2174888 2 135 gly Human 207:211 arg1 alpha-GalNAc Human alpha-N-acetylgalactosaminidase alpha-GalNAc PUBTATOR Human alpha-N-acetylgalactosaminidase 4668 Human alpha-N-acetylgalactosaminidase (alpha-GalNAc, E.C. 3.2.1.49), the lysosomal glycohydrolase that cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates, is encoded by a gene localized to chromosome 22q13----qter. 23426370 4 48 part_of Met 809:811 arg1 ssSPTb ssSPTb Met PUBTATOR SpecificSite ssSPTb 165679 Met(25) In combination with analysis of isoform chimera and site-directed mutagenesis, a single amino acid residue in this core (Met(25) in ssSPTa and Val(25) in ssSPTb) was identified which confers specificity for palmitoyl- or stearoyl-CoA, respectively, in both yeast and mammalian cells. 23426370 4 48 part_of Met 809:811 arg1 ssSPTa ssSPTa Met PUBTATOR SpecificSite ssSPTa 171546 Met(25) In combination with analysis of isoform chimera and site-directed mutagenesis, a single amino acid residue in this core (Met(25) in ssSPTa and Val(25) in ssSPTb) was identified which confers specificity for palmitoyl- or stearoyl-CoA, respectively, in both yeast and mammalian cells. 7980452 0 86 gly N-glycosylation 8:22 arg1 human interferon-gamma human interferon-gamma PUBTATOR interferon-gamma 3458 Role of N-glycosylation in the synthesis, dimerization and secretion of human interferon-gamma. 23714211 15 26 gly glycosylation 2577:2589 arg1 ICAM-2 ICAM-2 PUBTATOR ICAM-2 3384 CONCLUSIONS: Reduced glycosylation of ICAM-2 significantly attenuated, but did not abolish, its ability to suppress metastatic properties of NB cells. 20826563 13 17 gly glycosylated 1647:1658 arg1 glycosylated GH glycosylated GH Cterm GH Sialic acids seem to play a pivotal role for the properties of glycosylated GH. 2318210 10 70 gly rCD4 1593:1596 arg1 the carbohydrate structures rCD4 the carbohydrate structures PUBTATOR rCD4 24932 The constituent monosaccharides of the carbohydrate structures of rCD4 were found to be fucose, mannose, galactose, N-acetylglucosamine and N-acetylneuraminic acid. 8961954 12 11 gly nonglycosylated 1928:1942 arg1 The nonglycosylated hPTH/PTHrP receptor The nonglycosylated hPTH/PTHrP receptor PUBTATOR hPTH/PTHrP receptor 5745 The nonglycosylated hPTH/PTHrP receptor remains fully functional with regard to both of its known signal transduction pathways: cAMP-protein kinase A and phospholipase C-cytosolic calcium. 12490395 6 7 gly glycosylation 1070:1082 arg1 xcCAT1 xcCAT1 Cterm xcCAT1 114246 We found that xcCAT1 expressed in Chinese hamster ovary (CHO) cells undergoes less glycosylation than rCAT1 and that the expression of xcCAT1 rendered the CHO cells more susceptible to infection with Moloney MLV. 16877748 1 4 gly N-glycosylated 204:217 arg1 Intercellular adhesion molecule-1 Intercellular adhesion molecule-1 PUBTATOR Intercellular adhesion molecule-1 15894 Intercellular adhesion molecule-1 (ICAM-1) is a heavily N-glycosylated transmembrane protein comprising five extracellular Ig-like domains. 25502197 5 32 gly glycosylation 794:806 arg1 erythropoietin erythropoietin PUBTATOR erythropoietin 2056 The glycosylation of erythropoietin demonstrates the successful performance of posttranslational modifications in the novel cell-free systems. 21172408 0 74 gly factor 98:103 arg1 carbohydrates tissue factor carbohydrates OGER tissue factor P13726 Differences in the fractional abundances of carbohydrates of natural and recombinant human tissue factor. 27339896 7 40 gly glycoforms 1563:1572 arg1 uncleaved CBG uncleaved CBG OGER CBG P08185 Molecular dynamics simulations of various Asn(347) glycoforms of uncleaved CBG indicated that multiple Asn(347) glycan features are modulating the RCL digestion efficiencies by NE/PAE. 26018173 8 126 gly interactions 1583:1594 arg1 native Env trimers Env trimers interactions PUBTATOR Env trimers 100616444 These site-level studies are important for understanding antibody-glycan interactions on native Env trimers. 1705556 0 45 gly O-glycosylation 9:23 arg1 CD13 CD13 PUBTATOR CD13 290 Variable O-glycosylation of CD13 (aminopeptidase N). 8794331 14 59 gly unglycosylated 2636:2649 arg1 unglycosylated mutant mCAT-1 unglycosylated mutant mCAT-1 PUBTATOR mCAT-1 11987 Although infection with ecotropic MuLV had no effect on activity of the mink CAT-1 transporter that does not bind virus, it caused partial down-modulation of wild-type mCAT-1 and complete down-modulation of unglycosylated mutant mCAT-1. 3288503 1 24 gly N-glycosylation 110:124 arg1 human renin human renin PUBTATOR renin 5972 One or both of two putative N-glycosylation sites (at asparagine-5 and -75) of human renin was eliminated by amino acid replacement of the asparagine residue with an alanine residue using site-directed mutagenesis. 28187981 12 63 gly glycosylated 1542:1553 arg1 aberrant glycosylated MPO aberrant glycosylated MPO PUBTATOR MPO 4353 Thus, aberrant glycosylated MPO exposed neo-epitopes and was recognized by half of the patients with anti-GBM disease. 9557657 11 28 gly N-glycosylation 2333:2347 arg1 havcr-1 havcr-1 PUBTATOR havcr-1 26762 These results indicate that the Cys-rich region of havcr-1 and its first N-glycosylation site are required for binding of protective MAb 190/4 and HAV receptor function. 9259114 1 10 gly glycoprotein 84:95 arg1 GP GP Cterm GP Platelet glycoprotein (GP) Ib/IX/V complex is a major receptor for von Willebrand factor (vWF), which mediates platelet adhesion and aggregation under high shear stress conditions. 2168345 4 40 gly glycosylated 1041:1052 arg1 chemically glycosylated E. coli beta-galactosidase chemically glycosylated E. coli beta-galactosidase OGER beta-galactosidase P23780 A panel of 12 types of chemically glycosylated E. coli beta-galactosidase, exposing the pivotal carbohydrate residues for specific carbohydrate-dependent cell binding, was employed to study the expression of respective cell-surface sugar receptors on these cell lines. 19458237 3 57 gly CD24 578:581 arg1 Alpha2,3-sialyl residues CD24 Alpha2,3-sialyl residues PUBTATOR CD24 100133941 Alpha2,3-sialyl residues of CD24 bind to a structural motif in the first fibronectin type III domain of the adhesion molecule L1. 29562282 8 47 gly glycoprotein 1245:1256 arg1 NUP62 NUP62 OGER NUP62 P37198 Confocal imaging shows that AANL co-localizes extensively with NUP62, a heavily O-GlcNAcylated and abundant nuclear pore glycoprotein. 10988252 1 16 gly glycoprotein 181:192 arg1 The human epidermal growth factor receptor The human epidermal growth factor receptor PUBTATOR epidermal growth factor receptor 1956 The human epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein having 11 potential N-glycosylation sites in its extracellular domain. 8852492 8 33 gly glycosylation 1395:1407 arg1 IgAN IgAN PUBTATOR IgAN 60498 These results suggested that the increased reactivity of O-glycan(s) in the IgA1 hinge region to jacalin is due to an unusual glycosylation of serum IgA1 in IgAN. 8852492 8 33 gly glycosylation 1395:1407 arg1 serum IgA1 serum IgA1 PUBTATOR IgA1 3493 These results suggested that the increased reactivity of O-glycan(s) in the IgA1 hinge region to jacalin is due to an unusual glycosylation of serum IgA1 in IgAN. 27643667 8 62 gly deglycosylated 733:746 arg1 deglycosylated MPO deglycosylated MPO PUBTATOR MPO 4353 RESULTS: Compared with intact MPO, chlorination activity of deglycosylated MPO declined, in which removing of β-galactopyranoside (0.35 ± 0.02 vs. 0.50 ± 0.04, P < 0.001) and α-linked sialic acid (0.35 ± 0.02 vs. 0.50 ± 0.04, P < 0.001) presented the most significance. 27001418 4 38 gly glycoforms 871:880 arg1 MUC1 MUC1 PUBTATOR MUC1 4582 Using MUC1 as the model, this strategy can visualize distinct glycoforms of MUC1 on various cell types and quantitatively track terminal monosaccharide pattern. 22389722 1 5 gly glycosylated 174:185 arg1 hPAP hPAP PUBTATOR hPAP 5068 Secretory human prostatic acid phosphatase (hPAP) is glycosylated at three asparagine residues (N62, N188, N301) and has potent antinociceptive effects when administered to mice. 22389722 1 5 gly glycosylated 174:185 arg1 Secretory human prostatic acid phosphatase Secretory human prostatic acid phosphatase PUBTATOR prostatic acid phosphatase 55 Secretory human prostatic acid phosphatase (hPAP) is glycosylated at three asparagine residues (N62, N188, N301) and has potent antinociceptive effects when administered to mice. 23389953 0 78 gly TSH 57:59 arg1 Carbohydrate-mediated polyethylene glycol conjugation TSH Carbohydrate-mediated polyethylene glycol conjugation Cterm TSH Carbohydrate-mediated polyethylene glycol conjugation of TSH improves its pharmacological properties. 12022871 8 4 gly glycosylated 1241:1252 arg1 Human TGH Human TGH PUBTATOR Human TGH 1066 Human TGH was glycosylated in the insect cells. 25495042 4 4 gly glycosylation 679:691 arg1 TARP γ-2 TARP γ-2 PUBTATOR TARP γ-2 10369 We first showed that TARP γ-8 is an N-glycosylated protein, which contains two glycosylation sites, Asn53 and Asn56, and compared this with the glycosylation of TARP γ-2 and the AMPA receptor auxiliary protein CNIH-2 (cornichon homologue 2). 25495042 4 39 gly N-glycosylated 571:584 arg1 TARP γ-8 TARP γ-8 PUBTATOR TARP γ-8 59283 We first showed that TARP γ-8 is an N-glycosylated protein, which contains two glycosylation sites, Asn53 and Asn56, and compared this with the glycosylation of TARP γ-2 and the AMPA receptor auxiliary protein CNIH-2 (cornichon homologue 2). 26045554 5 45 gly N-glycosylated 1164:1177 arg1 the GluN2A and GluN2B subunits the GluN2A and GluN2B subunits PUBTATOR GluN2B subunits 2904 Although the GluN2A and GluN2B subunits are also N-glycosylated, their N-glycosylation sites do not appear to be essential for surface delivery of NMDARs. 21482747 8 15 gly nonglycosylated 1322:1336 arg1 The predominant intracellular form The predominant intracellular form PUBTATOR form of BNP 4879 The predominant intracellular form of BNP was nonglycosylated proBNP1-108, rather than BNP1-32. 28104755 0 80 gly N-glycosylation 0:14 arg1 human sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) human sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) PUBTATOR sphingomyelin phosphodiesterase acid-like 3A 10924 N-glycosylation of human sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is essential for stability, secretion and activity. 10682309 12 44 gly non-glycosylated 1874:1889 arg1 non-glycosylated SAP-B non-glycosylated SAP-B OGER SAP O60880 Feeding studies with non-glycosylated SAP-precursor, generating non-glycosylated SAP-B, showed that the loss of the carbohydrate chain reduced the intracellular activity of the protein significantly. 10682309 12 49 gly non-glycosylated 1831:1846 arg1 non-glycosylated SAP-precursor non-glycosylated SAP-precursor OGER SAP O60880 Feeding studies with non-glycosylated SAP-precursor, generating non-glycosylated SAP-B, showed that the loss of the carbohydrate chain reduced the intracellular activity of the protein significantly. 25495042 3 18 gly glycosylation 491:503 arg1 AMPA receptor trafficking AMPA receptor OGER AMPA receptor P19493 In the present study, we examined the effect of TARP glycosylation on AMPA receptor trafficking. 9641677 1 2 gly glycoprotein 276:287 arg1 the CD4 glycoprotein the CD4 glycoprotein PUBTATOR CD4 glycoprotein 920 The entry of human immunodeficiency virus (HIV) into cells requires the sequential interaction of the viral exterior envelope glycoprotein, gp120, with the CD4 glycoprotein and a chemokine receptor on the cell surface. 9641677 1 28 gly glycoprotein 242:253 arg1 gp120 gp120 PUBTATOR gp120 155971 The entry of human immunodeficiency virus (HIV) into cells requires the sequential interaction of the viral exterior envelope glycoprotein, gp120, with the CD4 glycoprotein and a chemokine receptor on the cell surface. 28630087 11 16 gly sialoglycoforms 1940:1954 arg1 the HNE sialoglycoforms the HNE sialoglycoforms PUBTATOR HNE 1991 Functionally, the paucimannosidic HNE glycoforms displayed preferential binding to human mannose binding lectin compared with the HNE sialoglycoforms, suggesting a glycoform-dependent involvement of HNE in complement activation. 28630087 11 30 gly glycoforms 1844:1853 arg1 the paucimannosidic HNE glycoforms the paucimannosidic HNE glycoforms PUBTATOR HNE 1991 Functionally, the paucimannosidic HNE glycoforms displayed preferential binding to human mannose binding lectin compared with the HNE sialoglycoforms, suggesting a glycoform-dependent involvement of HNE in complement activation. 9169007 1 3 gly glycoforms 166:175 arg1 recombinant antithrombin recombinant antithrombin PUBTATOR antithrombin 462 Two major glycoforms of recombinant antithrombin which differ 10-fold in their affinity for the effector glycosaminoglycan, heparin, were previously shown to be expressed in BHK or CHO mammalian cell lines (I. Björk, et al., 1992, Biochem. 1328682 4 32 gly glycoprotein 551:562 arg1 The UL16 glycoprotein The UL16 glycoprotein PUBTATOR UL16 glycoprotein 3077464 The UL16 glycoprotein was synthesized at early times after infection and accumulated to the highest levels at late times after infection. 8688424 11 29 gly fucosylation 1796:1807 arg1 antithrombin antithrombin PUBTATOR antithrombin 462 It is possible that fucosylation of antithrombin may occur in vivo as a means of modifying the physiological properties of the antithrombin through alteration of the amount of antithrombin bound to surface heparin-like species. 8688424 11 69 gly antithrombin 1812:1823 arg1 fucosylation antithrombin fucosylation PUBTATOR antithrombin 462 It is possible that fucosylation of antithrombin may occur in vivo as a means of modifying the physiological properties of the antithrombin through alteration of the amount of antithrombin bound to surface heparin-like species. 8207403 1 34 gly glycoprotein 225:236 arg1 gp41 gp41 Cterm gp41 The transmembrane envelope glycoprotein (gp41) of human immunodeficiency virus type 1 possesses four consensus sites (Asn-X-Ser/Thr) for the incorporation of N-linked sugars situated on the extracellular domain of the molecule. 19880378 9 20 gly N-glycosylation 1246:1260 arg1 POMT2 POMT2 PUBTATOR POMT2 29954 These results suggest that the N-glycosylation of POMT1 and POMT2 is required for maintaining the conformation as well as the activity of the POMT1-POMT2 complex. 19880378 9 20 gly N-glycosylation 1246:1260 arg1 POMT1 POMT1 PUBTATOR POMT1 10585 These results suggest that the N-glycosylation of POMT1 and POMT2 is required for maintaining the conformation as well as the activity of the POMT1-POMT2 complex. 25614955 7 25 gly glycoprotein 1238:1249 arg1 the glycoprotein G the glycoprotein G OGER glycoprotein G P07996 In addition, sequence analysis of the glycoprotein G identified an amino acid substitution (I338→T338) unique to the IMDRV-13 within antigenic sites III (330-338), this mutation also leads to an additional potential N-glycosylation site (N336), which may represent a useful model to study relationship of N-glycosylation in G protein and specific properties such as pathogenicity or host adaption of RABV. 25614955 7 66 gly N-glycosylation 1505:1519 arg1 G protein G protein OGER G protein In addition, sequence analysis of the glycoprotein G identified an amino acid substitution (I338→T338) unique to the IMDRV-13 within antigenic sites III (330-338), this mutation also leads to an additional potential N-glycosylation site (N336), which may represent a useful model to study relationship of N-glycosylation in G protein and specific properties such as pathogenicity or host adaption of RABV. 9054441 6 3 gly glycosylation 952:964 arg1 plasminogen 2 plasminogen 2 OGER plasminogen 2 P00747 In the present study, a combination of trypsin digestion, lectin affinity chromatography, Edman degradation amino acid sequence analysis, carbohydrate composition analysis, and mass spectrometry revealed the existence of a novel site for O-linked glycosylation on plasminogen 2 at Ser-248. 1850903 4 14 gly glycosylation 945:957 arg1 gG-1 gG-1 Cterm gG-1 gG-1 and gG-2 expressed in recombinant baculovirus-infected insect cells undergo cotranslational N-linked glycosylation, but the overall processing of the proteins differs from that observed in HSV-1 or HSV-2-infected cells. 1850903 4 14 gly glycosylation 945:957 arg1 gG-2 gG-2 Cterm gG-2 gG-1 and gG-2 expressed in recombinant baculovirus-infected insect cells undergo cotranslational N-linked glycosylation, but the overall processing of the proteins differs from that observed in HSV-1 or HSV-2-infected cells. 7706290 1 53 gly glycoprotein 124:135 arg1 Mouse Interleukin 4 Mouse Interleukin 4 PUBTATOR Mouse Interleukin 4 16189 Mouse Interleukin 4 is a 20-kDa glycoprotein, synthesized by activated T lymphocytes and mast cells, which regulates the growth and/or differentiation of a broad spectrum of target cells of the immune system, including B and T lymphocytes, macrophages, and hematopoietic progenitor cells. 27142834 0 44 gly O-glycosylated 4:17 arg1 FXYD5 FXYD5 PUBTATOR FXYD5 53827 The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase β1 subunits. 1897978 5 15 gly hGM-CSF 1006:1012 arg1 carbohydrate moieties hGM-CSF carbohydrate moieties PUBTATOR hGM-CSF 1437 To investigate the role of carbohydrate moieties of hGM-CSF, we isolated each form of hGM-CSF and examined its biological properties. 15557177 1 1 gly glycosylation 165:177 arg1 IgE IgE OGER IgE P01854 Analysis of the glycosylation of human serum IgD and IgE indicated that oligomannose structures are present on both Igs. 15557177 1 1 gly glycosylation 165:177 arg1 human serum IgD human serum IgD OGER IgD P01880 Analysis of the glycosylation of human serum IgD and IgE indicated that oligomannose structures are present on both Igs. 27173519 5 63 gly DSIgG 1221:1225 arg1 sialylation IgG sialylation Cterm IgG In addition, significant changes in galactosylation, sialylation, and bisecting N-acetylglucosamine (GlcNAc) from DSIgG were also observed between two pathophysiological states. 27173519 5 63 gly DSIgG 1221:1225 arg1 bisecting N-acetylglucosamine IgG bisecting N-acetylglucosamine Cterm IgG In addition, significant changes in galactosylation, sialylation, and bisecting N-acetylglucosamine (GlcNAc) from DSIgG were also observed between two pathophysiological states. 27173519 5 63 gly DSIgG 1221:1225 arg1 GlcNAc IgG GlcNAc Cterm IgG In addition, significant changes in galactosylation, sialylation, and bisecting N-acetylglucosamine (GlcNAc) from DSIgG were also observed between two pathophysiological states. 19136072 1 5 gly glycoprotein 328:339 arg1 Env Env PUBTATOR Env 100616444 Human immunodeficiency virus type 1 (HIV-1) env genes were cloned from blood samples of HIV-1-infected Thai patients, and 35 infectious CRF01_AE envelope glycoprotein (Env)-recombinant viruses were established. 19136072 1 5 gly glycoprotein 328:339 arg1 CRF01_AE envelope glycoprotein CRF01_AE envelope glycoprotein PUBTATOR CRF01_AE envelope glycoprotein 100616444 Human immunodeficiency virus type 1 (HIV-1) env genes were cloned from blood samples of HIV-1-infected Thai patients, and 35 infectious CRF01_AE envelope glycoprotein (Env)-recombinant viruses were established. 28081265 9 84 gly glycosylation 1324:1336 arg1 Pls Pls Cterm Pls Expression and deletion analysis revealed that both gtfC and gtfD mediate glycosylation of Pls. 18728239 6 27 gly desialylated 1117:1128 arg1 the desialylated BR3-Fc the desialylated BR3-Fc OGER BR3 Q96RJ3 The results of the data presented here suggest that exposed Gal on the desialylated BR3-Fc led to rapid clearance due to uptake and degradation in the liver that was associated with nonparenchymal cells. 18728239 6 92 gly Gal 1106:1108 arg1 the desialylated BR3-Fc BR3 Gal OGER BR3 Q96RJ3 The results of the data presented here suggest that exposed Gal on the desialylated BR3-Fc led to rapid clearance due to uptake and degradation in the liver that was associated with nonparenchymal cells. 2432614 0 43 gly glycoprotein 61:72 arg1 myelin-associated glycoprotein myelin-associated glycoprotein PUBTATOR structure of myelin-associated glycoprotein 29409 Molecular cloning and primary structure of myelin-associated glycoprotein. 18027437 5 25 gly present 736:742 arg2 MUC1 AND the carbohydrates MUC1 the carbohydrates PUBTATOR MUC1 4582 The characteristics of the MUCI epitopes constituted with the tandem repeats and the carbohydrates present on MUC1 induce immune responses that favor targeted immunotherapy. 17980170 0 42 gly glycosylation 27:39 arg1 N-acylethanolamine-hydrolyzing acid amidase N-acylethanolamine-hydrolyzing acid amidase PUBTATOR N-acylethanolamine-hydrolyzing acid amidase 27163 Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism. 15026421 0 73 gly glycoprotein 146:157 arg1 P-selectin glycoprotein ligand-1 P-selectin glycoprotein ligand-1 PUBTATOR P-selectin glycoprotein ligand-1 6404 Functional analysis of the combined role of the O-linked branching enzyme core 2 beta1-6-N-glucosaminyltransferase and dimerization of P-selectin glycoprotein ligand-1 in rolling on P-selectin. 8349598 7 73 gly acceptors 1590:1598 arg1 Trf Trf acceptors PUBTATOR Trf 7018 In contrast Bowes, EL-4, and B16L6 cell extracts transferred GalNAc to oligosaccharides acceptors on Trf but not on hCG. 8349598 7 73 gly acceptors 1590:1598 arg1 hCG hCG acceptors OGER hCG In contrast Bowes, EL-4, and B16L6 cell extracts transferred GalNAc to oligosaccharides acceptors on Trf but not on hCG. 8063760 7 31 gly attached 1026:1033 arg2 AP-3 AND single GlcNAc residues AP-3 single GlcNAc residues OGER AP-3 Analysis of the disaccharide released by beta-elimination indicated that single GlcNAc residues are attached to AP-3 through an O-glycosidic linkage to threonine or serine residues. 2174119 6 6 gly glycosylated 1214:1225 arg1 glycosylated Fc glycosylated Fc Cterm Fc The monomeric aglycosylated trypsin Fc fragment inhibits human Fc gamma R1 recognition by U937 cells 115-fold less well (K50 = 2 microM) than does glycosylated Fc (K50 = 17 nM), confirming that aglycosylation disrupts the site for human Fc gamma R1 within the CH2 domain and indicating that the trypsin Fc fragments reflect the functional properties of the intact IgG glycoforms. 2174119 6 32 gly glycoforms 1435:1444 arg1 the intact IgG glycoforms the intact IgG glycoforms Cterm IgG The monomeric aglycosylated trypsin Fc fragment inhibits human Fc gamma R1 recognition by U937 cells 115-fold less well (K50 = 2 microM) than does glycosylated Fc (K50 = 17 nM), confirming that aglycosylation disrupts the site for human Fc gamma R1 within the CH2 domain and indicating that the trypsin Fc fragments reflect the functional properties of the intact IgG glycoforms. 15100290 5 46 gly present 628:634 arg2 CDR1 AND another carbohydrate CDR1 another carbohydrate PUBTATOR CDR1 1038 Also, when another carbohydrate was present in CDR1, CDR2, or CDR3 of the L chain, the V(H) CDR2 glycan remained high mannose. 15100290 5 46 gly present 628:634 arg1 CDR2 AND another carbohydrate CDR2 another carbohydrate OGER CDR2 P97817 Also, when another carbohydrate was present in CDR1, CDR2, or CDR3 of the L chain, the V(H) CDR2 glycan remained high mannose. 15100290 5 46 gly present 628:634 arg1 CDR3 AND another carbohydrate CDR3 another carbohydrate PUBTATOR CDR3 8163 Also, when another carbohydrate was present in CDR1, CDR2, or CDR3 of the L chain, the V(H) CDR2 glycan remained high mannose. 8645991 3 30 gly residues 542:549 arg1 ASN 99 ASN 99 ASN 99 SpecificSite ASN 99 However, in mass spectrometry the sugar residues on ASN 99 (219) and 252 (371) were deficient in sialic acids. 23389048 2 65 gly glycoforms 321:330 arg1 the disease-related haptoglobin glycoforms the disease-related haptoglobin glycoforms PUBTATOR haptoglobin 3240 To analyze the disease-related haptoglobin glycoforms in liver cirrhosis and hepatocellular carcinoma, we have optimized an LC-MS-multiple reaction monitoring (MRM) workflow for glycopeptide quantification. 1413513 2 45 gly glycosylated 500:511 arg1 SHp SHp PUBTATOR SHp 8431 Analysis of SH protein expressed in cells infected with RS virus or with a recombinant vaccinia virus revealed two glycosylated SH protein species, SHg and SHp, which contained N-linked carbohydrate residues. 1413513 2 31 gly contained 552:560 arg1 SHp AND N-linked carbohydrate residues SHp N-linked carbohydrate residues PUBTATOR SHp 8431 Analysis of SH protein expressed in cells infected with RS virus or with a recombinant vaccinia virus revealed two glycosylated SH protein species, SHg and SHp, which contained N-linked carbohydrate residues. 15743766 5 63 gly carbohydrate 867:878 arg1 Fcepsilon3-4 Fcepsilon3-4 carbohydrate Cterm Fcepsilon3-4 In contrast to IgG Fc where deglycosylation abrogates receptor binding activity, the removal of the N-linked carbohydrate at Asn-394 in Fcepsilon3-4 only reduces binding affinity by a factor of 4, principally because of a faster off-rate. 28400175 3 104 gly N-glycosylation 442:456 arg1 recombinant IgE recombinant IgE OGER IgE P01854 Here, we present the expression of recombinant IgE in wild type and glycan-engineered Nicotiana benthamiana plants and in-depth N-glycosylation analyses. 9587408 2 19 gly glycoprotein 309:320 arg1 TXA2R TXA2R PUBTATOR TXA2R 6915 TXA2R was shown to be a glycoprotein. 17522223 2 1 gly sites 361:365 arg1 gp120 gp120 sites PUBTATOR gp120 3700 Here, we delineate the N-linked glycosylation (N-glycan) sites in gp120 that contribute to optimal DC-SIGN binding. 2174119 5 40 gly glycosylated 760:771 arg1 the glycosylated intact IgG3 the glycosylated intact IgG3 PUBTATOR IgG3 3502 Functionally, whilst the glycosylated intact IgG3 interacts with human Fc gamma R111 expressed on human killer (K) cells to trigger antibody-dependent cellular cytotoxicity the aglycosylated intact IgG3 fails to trigger cell lysis, localising the site on IgG for triggering human Fc gamma R111 mediated functions to the CH2 domain. 2174119 5 83 gly aglycosylated 912:924 arg1 the aglycosylated intact IgG3 the aglycosylated intact IgG3 PUBTATOR IgG3 3502 Functionally, whilst the glycosylated intact IgG3 interacts with human Fc gamma R111 expressed on human killer (K) cells to trigger antibody-dependent cellular cytotoxicity the aglycosylated intact IgG3 fails to trigger cell lysis, localising the site on IgG for triggering human Fc gamma R111 mediated functions to the CH2 domain. 8757998 0 63 gly glycoprotein 36:47 arg1 human cytomegalovirus glycoprotein B human cytomegalovirus glycoprotein B Cterm human cytomegalovirus glycoprotein B Processing of human cytomegalovirus glycoprotein B in recombinant adenovirus-infected cells. 15536627 0 42 gly transferrin 46:56 arg1 Site-specific carbohydrate profiling transferrin Site-specific carbohydrate profiling PUBTATOR transferrin 7018 Site-specific carbohydrate profiling of human transferrin by nano-flow liquid chromatography/electrospray ionization mass spectrometry. 7685965 7 47 gly deglycosylation 1118:1132 arg1 HA1 HA1 OGER HA1 More extensive deglycosylation of HA1 had a differential effect on the clones, allowing one to proliferate but not the other. 21762534 4 14 gly ECI 452:454 arg1 the shortest sugar ECI the shortest sugar OGER ECI Q9BS40 We investigated whether synthetic ECI with the shortest sugar is functionally active. 28708860 3 12 gly N-glycosylation 462:476 arg1 NA NA PUBTATOR NA 4758 The purpose of this paper is to explore the relations between charged amino acids, N-glycosylation and epitopes in hemagglutinin (HA) and neuraminidase (NA). 28708860 3 12 gly N-glycosylation 462:476 arg1 HA HA Cterm HA The purpose of this paper is to explore the relations between charged amino acids, N-glycosylation and epitopes in hemagglutinin (HA) and neuraminidase (NA). 28708860 3 12 gly N-glycosylation 462:476 arg1 neuraminidase neuraminidase PUBTATOR neuraminidase 4758 The purpose of this paper is to explore the relations between charged amino acids, N-glycosylation and epitopes in hemagglutinin (HA) and neuraminidase (NA). 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND galactose the tumor necrosis factor-alpha galactose PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND N-acetylneuraminic acid the tumor necrosis factor-alpha N-acetylneuraminic acid PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND N-acetylgalactosamine the tumor necrosis factor-alpha N-acetylgalactosamine PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 8631363 3 11 gly contained 418:426 arg1 the tumor necrosis factor-alpha AND sugar components the tumor necrosis factor-alpha sugar components PUBTATOR tumor necrosis factor-alpha 7124 Sugar composition analyses revealed that the tumor necrosis factor-alpha contained galactose, N-acetylgalactosamine and N-acetylneuraminic acid as sugar components. 1969925 8 44 gly heterogeneity 750:762 arg1 PrP PrP PUBTATOR PrP 5621 Some of the heterogeneity of PrP is therefore due to differential N-glycosylation. 10356976 5 13 gly N-glycans 727:735 arg1 corticosteroid binding globulin corticosteroid binding globulin N-glycans OGER corticosteroid binding globulin P08185 Thus, the N-glycans on corticosteroid binding globulin do not act as an apical sorting signal in MDCK cells. 12637583 3 1 gly SIgA1 417:421 arg1 all its glycans IgA1 all its glycans Cterm IgA1 Based on these data, we have constructed a molecular model of SIgA1 with all its glycans, in which the Fab arms form a T shape and the SC is wrapped around the heavy chains. 15147907 2 0 gly presence 333:340 arg2 the human complement serum glycoprotein C3 AND such monoglucosylated N-glycans the human complement serum glycoprotein C3 such monoglucosylated N-glycans Cterm C3 We report here the presence of such monoglucosylated N-glycans on the human complement serum glycoprotein C3. 9718580 0 39 gly glycosylation 48:60 arg1 recombinant human coagulation factor VIIa recombinant human coagulation factor VIIa Cterm factor VIIa Analysis of the site-specific asparagine-linked glycosylation of recombinant human coagulation factor VIIa by glycosidase digestions, liquid chromatography, and mass spectrometry. 29759137 4 40 gly glycosylation 407:419 arg1 IgG IgG Cterm IgG However, limited data is available on the glycosylation pattern of IgG in cerebrospinal fluid (CSF) compared to serum. 9450956 5 36 gly nonglycosylated 561:575 arg1 nonglycosylated RNase 4 nonglycosylated RNase 4 PUBTATOR RNase 4 6038 Expression of chimeras of RNase 2 and nonglycosylated RNase 4 and deletion mutants in HEK293 cells identified residues 1-13 to be sufficient for C-mannosylation. 3950419 6 86 gly contains 1278:1285 arg1 GPBch AND the 10F7 determinant GPBch the 10F7 determinant Cterm GPBch 2994 GPBch has higher apparent m.w. than human GPB, is present in the erythrocyte membrane in greater quantity than human GPB, and contains trypsin cleavage site(s) and the 10F7 determinant (both found on human GPA but not GPB). 3636155 7 52 gly contain 1298:1304 arg1 Each heavy chain AND 4 tandem repeats Each heavy chain 4 tandem repeats OGER chain 3818 Each heavy chain of factor XIa (369 amino acids) was found to contain 4 tandem repeats of 90 (or 91) amino acids plus a short connecting peptide. 2608056 8 6 gly glycosylation 1960:1972 arg1 free LH beta free LH beta PUBTATOR LH beta 280839 Collectively, these results indicate that N-linked glycosylation is important for intracellular degradation of free LH beta, but is not essential for either its assembly with alpha-subunit or the transport and secretion of biologically active heterodimer. 17195076 1 10 gly glycoprotein 164:175 arg1 The epithelial mucin MUC1 The epithelial mucin MUC1 OGER MUC1 P15941 The epithelial mucin MUC1 is a high molecular weight membrane glycoprotein frequently overexpressed and aberrantly glycosylated in adenocarcinoma. 17195076 1 11 gly glycosylated 217:228 arg1 The epithelial mucin MUC1 The epithelial mucin MUC1 OGER MUC1 P15941 The epithelial mucin MUC1 is a high molecular weight membrane glycoprotein frequently overexpressed and aberrantly glycosylated in adenocarcinoma. 29153507 1 49 gly glycoprotein 122:133 arg1 Afamin Afamin PUBTATOR Afamin 173 Afamin, a human plasma glycoprotein and putative transporter of hydrophobic molecules, has been shown to act as extracellular chaperone for poorly soluble, acylated Wnt proteins, forming a stable, soluble complex with functioning Wnt proteins. 7605197 0 49 gly glycoprotein 4:15 arg1 The glycoprotein G The glycoprotein G OGER glycoprotein G P07996 The glycoprotein G of rhabdoviruses. 11251288 11 97 gly glycosylated 2326:2337 arg1 Erythropoietin Erythropoietin PUBTATOR Erythropoietin 2056 CONCLUSIONS: Erythropoietin expressed in E. coli bearing specific Asn-->Cys mutations at natural glycosylation sites can be glycosylated using beta-N-glycosyl iodoacetamides even in the presence of two disulfide bonds. 7914388 9 24 gly N-glycosylation 1540:1554 arg1 hPVR function hPVR function PUBTATOR hPVR 5817 N-glycosylation of the four sites in domains 1 and 2 is not required for hPVR function, but glycosylation in domain 1 has a greater effect on receptor function than that of domain 2. 10066782 0 57 gly N-glycosylation 29:43 arg1 the human interleukin-6 receptor the human interleukin-6 receptor PUBTATOR interleukin-6 receptor 3570 Disulfide bond structure and N-glycosylation sites of the extracellular domain of the human interleukin-6 receptor. 19556306 6 18 gly TLR4 975:978 arg1 complex type N-glycans TLR4 complex type N-glycans PUBTATOR TLR4 7099 The amount of the 130 kDa TLR4(C88A) with complex type N-glycans expressed on the cell surface depended on that of MD-2 transfected. 18981290 0 37 gly ADAMTS13 13:20 arg1 N-Glycans ADAMTS13 N-Glycans PUBTATOR ADAMTS13 100770010 N-Glycans of ADAMTS13 modulate its secretion and von Willebrand factor cleaving activity. 19379732 3 5 gly glycosylated 512:523 arg1 WT hSVCT1 WT hSVCT1 PUBTATOR WT hSVCT1 9963 PNGase F treatment confirmed that WT hSVCT1 (approximately 70-100 kDa) is glycosylated and site-directed mutagenesis of the three putative N-glycosylation sites, Asn138, Asn144, Asn230, demonstrated that mutants N138Q and N144Q were glycosylated (approximately 68-90 kDa) with only 31-65% of WT l-ascorbic acid (AA) uptake while the glycosylation profile of N230Q remained unaltered (approximately 98% of WT activity). 10858228 5 46 gly glycoprotein 838:849 arg1 gp15 gp15 Cterm gp15 We have cloned and sequenced a gene designated Cpgp40/15 that encodes gp40 as well as gp15, an antigenically distinct, surface glycoprotein also implicated in C. parvum-host cell interactions. 11563913 12 14 gly glycosylated 2094:2105 arg1 full-length glycosylated human BSSL full-length glycosylated human BSSL PUBTATOR BSSL 1056 We have also solved the structure of full-length glycosylated human BSSL at 4.1 A resolution, using the refined coordinates of the truncated molecule as a search model. 26946944 1 51 gly glycosylated 89:100 arg1 CTL CTL Cterm CTL A glycosylated lectin (CTL) with specificity for mannose and glucose has been detected and purified from seeds of Centrolobium tomentosum, a legume plant from Dalbergieae tribe. 7753821 4 23 gly glycosylation 601:613 arg1 the oncoprotein c-Myc the oncoprotein c-Myc OGER Myc P01106 Here we present evidence for O-GlcNAc glycosylation of the oncoprotein c-Myc, a helix-loop-helix/leucine zipper phosphoprotein that heterodimerizes with Max and participates in the regulation of gene transcription in normal and neoplastic cells. 7753821 4 62 gly c-Myc 634:638 arg1 O-GlcNAc glycosylation Myc O-GlcNAc glycosylation OGER Myc P01106 Here we present evidence for O-GlcNAc glycosylation of the oncoprotein c-Myc, a helix-loop-helix/leucine zipper phosphoprotein that heterodimerizes with Max and participates in the regulation of gene transcription in normal and neoplastic cells. 29587225 3 51 gly sialylation 439:449 arg1 EPO conformation EPO conformation PUBTATOR EPO 2056 Nonetheless, a detailed understanding of the effects of sialylation on EPO conformation and dynamics is still lacking. 8084592 1 52 gly glycoprotein 191:202 arg1 MN MN Cterm MN MN is a transmembrane glycoprotein that has been detected in HeLa cells and in some human carcinomas. 8647865 9 26 gly located 1508:1514 arg2 MAP2 AND both the O-GlcNAc and biotin hydrazide-reactive carbohydrate moieties MAP2 both the O-GlcNAc and biotin hydrazide-reactive carbohydrate moieties PUBTATOR MAP2 25595 We have also shown that both the O-GlcNAc and biotin hydrazide-reactive carbohydrate moieties are located on the projection domain of MAP2. 12871464 5 2 gly carbohydrate 827:838 arg1 Asn 45 Asn 45 Asn 45 SpecificSite Asn 45 The N-linked carbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator. 12706347 4 9 part_of asparagine 691:700 arg1 NTPDase3 NTPDase3 asparagine PUBTATOR SpecificSite NTPDase3 956 sites, asparagine 81 Only one of these putative glycosylation sites, asparagine 81 in NTPDase3, which is located near apyrase conserved region 1 (ACR1), is invariant in all the cell surface membrane eNTPDases. 22387313 0 42 gly N-glycosylation 0:14 arg1 the mammalian dipeptidyl aminopeptidase-like protein 10 the mammalian dipeptidyl aminopeptidase-like protein 10 PUBTATOR dipeptidyl aminopeptidase-like protein 10 57628 N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. 22387313 0 42 gly N-glycosylation 0:14 arg1 DPP10 DPP10 PUBTATOR DPP10 57628 N-glycosylation of the mammalian dipeptidyl aminopeptidase-like protein 10 (DPP10) regulates trafficking and interaction with Kv4 channels. 20506028 0 65 gly glycosylated 25:36 arg1 chondromodulin-I chondromodulin-I PUBTATOR chondromodulin-I 11061 A functional role of the glycosylated N-terminal domain of chondromodulin-I. 15477100 0 64 gly Zn-alpha2-glycoprotein 46:67 arg1 Zn-alpha2-glycoprotein Zn-alpha2-glycoprotein PUBTATOR Zn-alpha2-glycoprotein 563 Crystallographic studies of ligand binding by Zn-alpha2-glycoprotein. 11179479 3 55 gly mucin 468:472 arg1 the tandem repeat domain mucin the tandem repeat domain PUBTATOR mucin 100508689 Their epitopes can be classified according to their position within the tandem repeat domain of the mucin and with respect to effects exerted by site-specific glycosylation. 2706086 6 36 gly N-glycosylation 997:1011 arg1 LAP LAP PUBTATOR LAP 53 Only two out of 8 N-glycosylation sites in LAP and 3 in PAP are conserved, suggesting that the dense N-glycosylation of LAP is related to its function in lysosomes. 19284292 2 23 gly polysialylation 406:420 arg1 NCAM NCAM PUBTATOR NCAM 4684 Examples of the latter are found in the formation of the mannose-6-phosphate receptor ligand on lysosomal hydrolases, and in polysialylation of NCAM, which are regulated via conformational signal patches on the protein. 19284292 2 45 gly NCAM 425:428 arg1 polysialylation NCAM polysialylation PUBTATOR NCAM 4684 Examples of the latter are found in the formation of the mannose-6-phosphate receptor ligand on lysosomal hydrolases, and in polysialylation of NCAM, which are regulated via conformational signal patches on the protein. 8660696 9 26 gly N-glycosylation 1539:1553 arg1 APP itself APP Cterm APP These results indicate that defective N-glycosylation of other cellular proteins, but not of APP itself, affects the metabolism of APP. 22243251 11 42 gly has 1323:1325 arg1 pig KLK4 AND NA3 N-glycan cores pig KLK4 NA3 N-glycan cores PUBTATOR KLK4 56640 We conclude that pig KLK4 has NA2, NA2F, and NA3 N-glycan cores with no, or with one, two, or three sialic acids. 12090474 1 86 gly glycoprotein 228:239 arg1 MUC1 MUC1 PUBTATOR MUC1 4582 MUC1 is a transmembrane glycoprotein abundantly expressed on the apical surface of human ductal epithelial cells and over entire cell surface of tumors originating from those cells. 21715597 0 13 gly glycans 37:43 arg1 gp120 gp120 glycans PUBTATOR gp120 155971 Removal of two high-mannose N-linked glycans on gp120 renders human immunodeficiency virus 1 largely resistant to the carbohydrate-binding agent griffithsin. 15140192 10 22 gly glycosylation 1207:1219 arg1 prestin prestin PUBTATOR prestin 375611 N-linked glycosylation is not required for plasma membrane targeting of prestin. 7755594 1 0 gly glycoprotein 158:169 arg1 Human interferon-gamma Human interferon-gamma PUBTATOR Human interferon-gamma 3458 Human interferon-gamma (IFN-gamma) is a secretory, dimeric glycoprotein that forms a compact globular structure with potential N-linked glycosylation sites at Asn-25 and Asn-97 on the surface of the dimer. 22243251 1 14 gly glycosylated 110:121 arg1 Kallikrein-related peptidase 4 Kallikrein-related peptidase 4 PUBTATOR Kallikrein-related peptidase 4 56640 Kallikrein-related peptidase 4 (KLK4) is a glycosylated serine protease that functions in the maturation (hardening) of dental enamel. 12223479 12 107 gly glycosylation 2013:2025 arg1 its auxiliary beta4 subunit its auxiliary beta4 subunit PUBTATOR beta4 subunit 10717 Taken together, these data show that the pore-forming alpha subunit of the hSlo channel promotes N-linked glycosylation of its auxiliary beta4 subunit, and this in turn influences the modulation of the channel by the beta4 subunit. 16227292 7 83 gly glycans 1148:1154 arg1 N1169 N1169 N1169 SpecificSite N1169 In contrast, mutant Gc proteins lacking glycans on either N624 or N1169, or both sites, were able to target to the Golgi. 16227292 7 83 gly glycans 1148:1154 arg1 N624 N624 N624 SpecificSite N624 In contrast, mutant Gc proteins lacking glycans on either N624 or N1169, or both sites, were able to target to the Golgi. 17486637 9 91 gly glycosylation 1565:1577 arg1 ZP4 ZP4 PUBTATOR ZP4 57829 Employing baculovirus-expressed recombinant ZP3 and ZP4 with reduced N-linked glycosylation and respective E. coli-expressed recombinant proteins, it was observed that glycosylation is required for induction of acrosomal exocytosis but its absence may not compromise on their binding ability. 17486637 9 91 gly glycosylation 1565:1577 arg1 baculovirus-expressed recombinant ZP3 baculovirus-expressed recombinant ZP3 PUBTATOR ZP3 7784 Employing baculovirus-expressed recombinant ZP3 and ZP4 with reduced N-linked glycosylation and respective E. coli-expressed recombinant proteins, it was observed that glycosylation is required for induction of acrosomal exocytosis but its absence may not compromise on their binding ability. 27539975 7 57 gly glycoproteins 1472:1484 arg1 MFGM glycoproteins biosynthesis MFGM glycoproteins biosynthesis PUBTATOR MFGM glycoproteins 4240 The findings increased the number of known N-glycosylation sites in the milk from dairy animal species, revealed the complexity of the MFGM glycoproteome, and provided useful information to further explore the mechanism of MFGM glycoproteins biosynthesis among the studied mammals. 8726871 1 25 gly glycoprotein 122:133 arg1 oEGP oEGP Cterm oEGP Ovine oestrus-associated oviducal glycoprotein (oEGP) is synthesized and secreted specifically by the ampullary region of the ovine oviduct during the peri-ovulatory stages of the oestrous cycle. 9593713 8 47 gly glycosylation 1351:1363 arg1 the ATPase the ATPase OGER ATPase Transient transfection resulted in most of the protein being retained in the endoplasmic reticulum with only core glycosylation and minor activity of the ATPase evident. 6284780 0 77 gly glycosylation 14:26 arg1 proopiomelanocortin proopiomelanocortin PUBTATOR proopiomelanocortin 5443 Synthesis and glycosylation of proopiomelanocortin by a Cushing tumor. 7584619 1 5 gly glycoprotein 109:120 arg1 Transferrin Transferrin PUBTATOR Transferrin 7018 Transferrin is a glycoprotein functioning in iron transport in higher eukaryotes, and consists of two highly homologous domains. 2507634 6 0 gly aglycosylated 1101:1113 arg1 aglycosylated IgG3-Gln aglycosylated IgG3-Gln PUBTATOR AminoAcid IgG3 380795 The serum half-life in mice of aglycosylated IgG1-Gln remains the same as wild-type IgG1, 6.5 +/- 0.5 days, whereas aglycosylated IgG3-Gln has a shorter half-life, 3.5 +/- 0.2 days, compared to that of wild-type IgG3, 5.1 +/- 0.4 days. 2507634 6 41 gly aglycosylated 1016:1028 arg1 aglycosylated IgG1-Gln aglycosylated IgG1-Gln PUBTATOR AminoAcid IgG1 16017 The serum half-life in mice of aglycosylated IgG1-Gln remains the same as wild-type IgG1, 6.5 +/- 0.5 days, whereas aglycosylated IgG3-Gln has a shorter half-life, 3.5 +/- 0.2 days, compared to that of wild-type IgG3, 5.1 +/- 0.4 days. 14634141 1 68 gly glycoprotein 400:411 arg1 desmoglein 1 desmoglein 1 PUBTATOR desmoglein 1 1828 Pemphigus foliaceus (PF) is a life-threatening autoimmune blistering skin disease caused by pathogenic IgG autoantibodies against desmoglein 1 (dg1), a desmosomal cadherin-type adhesion glycoprotein. 11856322 0 51 gly low-glycosylated 31:46 arg1 human butyrylcholinesterase human butyrylcholinesterase PUBTATOR butyrylcholinesterase 590 Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase: expression, purification, characterization and crystallization. 26972002 1 34 gly glycoprotein 123:134 arg1 The HIV-1 envelope glycoprotein The HIV-1 envelope glycoprotein PUBTATOR HIV-1 envelope glycoprotein trimer 155971 The HIV-1 envelope glycoprotein trimer is covered by an array of N-linked glycans that shield it from immune surveillance. 24337294 1 1 gly glycoprotein 76:87 arg1 Erythropoietin Erythropoietin PUBTATOR Erythropoietin 2056 Erythropoietin is a signaling glycoprotein that controls the fundamental process of erythropoiesis, orchestrating the production and maintenance of red blood cells. 17660514 0 54 gly glycosylation 8:20 arg1 corin zymogen activation corin zymogen activation PUBTATOR corin 10699 Role of glycosylation in corin zymogen activation. 9722984 3 73 gly glycoprotein 489:500 arg1 the mucin glycoprotein molecule the mucin glycoprotein molecule PUBTATOR mucin glycoprotein 100508689 The MUC2 gene product is more than 5100 amino acids in its commonest allelic form and accounts for one fifth by weight of the mucin glycoprotein molecule (80% oligosaccharide side chains). 21733844 7 45 gly glycosylated 988:999 arg1 glycosylated hAQP10 glycosylated hAQP10 PUBTATOR hAQP10 89872 In contrast, glycosylated hAQP10 showed increased thermostability of 3-6 °C compared with the nonglycosylated protein, suggesting a stabilizing effect of the N-linked glycan. 27773703 18 6 gly glycoforms 1759:1768 arg1 megalin megalin PUBTATOR megalin 14725 Cell type-specific glycoforms of megalin exist in the proximal tubular cells and modulate ligand absorption capacity. 7690818 10 2 gly aglycosylated 1374:1386 arg1 aglycosylated IgG4 aglycosylated IgG4 OGER IgG4 P01861 Some RA-derived RF bound aglycosylated IgG4 less well than glycosylated IgG4, suggesting that the carbohydrate moiety was important in establishing their binding epitope in CH2. 7690818 10 67 gly glycosylated 1408:1419 arg1 glycosylated IgG4 glycosylated IgG4 OGER IgG4 P01861 Some RA-derived RF bound aglycosylated IgG4 less well than glycosylated IgG4, suggesting that the carbohydrate moiety was important in establishing their binding epitope in CH2. 7987212 7 71 gly glycosylated 859:870 arg1 Rat C5a Rat C5a PUBTATOR Rat C5a 728 Rat C5a, like human C5a, is glycosylated but contains 77 amino acid residues instead of the 74 residues of human C5a. 19880378 7 0 gly POMT1 1095:1099 arg1 all N-glycosylation sites POMT1 all N-glycosylation sites PUBTATOR POMT1 10585 Mutation of any single site did not significantly affect POMT activity, but mutations of all N-glycosylation sites of either POMT1 or POMT2 caused a loss of POMT activity. 19880378 7 18 gly N-glycosylation 1063:1077 arg1 POMT1 POMT1 PUBTATOR POMT1 10585 Mutation of any single site did not significantly affect POMT activity, but mutations of all N-glycosylation sites of either POMT1 or POMT2 caused a loss of POMT activity. 19880378 7 18 gly N-glycosylation 1063:1077 arg1 POMT2 POMT2 PUBTATOR POMT2 29954 Mutation of any single site did not significantly affect POMT activity, but mutations of all N-glycosylation sites of either POMT1 or POMT2 caused a loss of POMT activity. 19880378 7 43 gly POMT2 1104:1108 arg1 all N-glycosylation sites POMT2 all N-glycosylation sites PUBTATOR POMT2 29954 Mutation of any single site did not significantly affect POMT activity, but mutations of all N-glycosylation sites of either POMT1 or POMT2 caused a loss of POMT activity. 9597548 5 34 gly presence 900:907 arg1 this human IgM AND oligosaccharides this human IgM oligosaccharides OGER IgM P01871 Of note is the presence in this human IgM of oligosaccharides containing N-glycolylneuraminic acid and N-acetylneuraminic acid in the ratio of 98:2 as determined using anion-exchange chromatography. 18992821 1 53 gly glycoprotein 134:145 arg1 The beta 2 integrin CR3 The beta 2 integrin CR3 OGER CR3 The beta 2 integrin CR3 is a leukocyte adhesion heterodimeric glycoprotein which functions both as receptor for iC3b and in several cell-cell and cell-substrate adhesion interactions. 2170216 0 40 gly glycosylation 13:25 arg1 HDL HDL OGER HDL Q9UNE0 Nonenzymatic glycosylation of HDL resulting in inhibition of high-affinity binding to cultured human fibroblasts. 24760753 11 11 gly sites 1600:1604 arg1 sOGT sOGT (S52 sites OGER sOGT (S52 Q9NZV1 To test the rule, 2 O-GlcNAcylation sites on sOGT (S52 and T449) were predicted and confirmed by Western blot. 12693993 11 71 gly glycosylated 1672:1683 arg1 human CRP human CRP PUBTATOR CRP 1401 Thus we have convincingly demonstrated that human CRP is glycosylated in some pathological conditions. 17208043 12 7 gly N-glycosylation 1647:1661 arg1 ATX ATX PUBTATOR ATX 18606 In conclusion, the present work demonstrates the crucial role of N-glycosylation in secretion and activity of ATX. 9514732 1 40 gly glycoprotein 115:126 arg1 Fibronectin Fibronectin PUBTATOR Fibronectin 2335 Fibronectin is an extracellular matrix glycoprotein that plays a role in a number of physiological processes involving cell adhesion and migration. 15616123 2 30 gly glycosylation 356:368 arg1 apoB100 apoB100 PUBTATOR apoB100 338 In the present study, a site-specific glycosylation analysis of apoB100 was carried out using reversed-phase high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI MS/MS). 1318394 11 51 gly nonglycosylated 1288:1302 arg1 These three nonglycosylated MHVR proteins These three nonglycosylated MHVR proteins PUBTATOR MHVR proteins 26365 These three nonglycosylated MHVR proteins were recognized by polyclonal antibody against affinity-purified receptor but did not bind antireceptor monoclonal antibody (MAb) CC1 or MHV-A59 virions. 10541351 0 117 gly glycosylation 32:44 arg1 a humanized IgG1 immunoglobulin a humanized IgG1 immunoglobulin PUBTATOR IgG1 immunoglobulin 16017 The effects of domain deletion, glycosylation, and long IgG3 hinge on the biodistribution and serum stability properties of a humanized IgG1 immunoglobulin, hLL2, and its fragments. 25211026 14 33 gly sialidase 2005:2013 arg1 lysosomal sialidase Neu1 lysosomal sialidase lysosomal sialidase Neu1 OGER lysosomal sialidase Q99519 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 43 gly PrP 2144:2146 arg1 desialylation PrP desialylation PUBTATOR PrP 19122 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 55 gly sialylation 2039:2049 arg1 C C Cterm C 19122 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 55 gly sialylation 2039:2049 arg1 brain-derived PrP brain-derived PrP PUBTATOR PrP 19122 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 82 gly PrP 2075:2077 arg1 the sialylation status PrP the sialylation status PUBTATOR PrP 19122 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 91 gly desialylation 2127:2139 arg1 PrP PrP PUBTATOR PrP 19122 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 91 gly desialylation 2127:2139 arg1 C C Cterm C 19122 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 25211026 14 116 gly lysosomal 1995:2003 arg1 lysosomal sialidase Neu1 lysosomal sialidase lysosomal sialidase Neu1 OGER lysosomal sialidase Q99519 Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C). 18341291 1 60 gly glycosylation 176:188 arg1 H P-type ATPases Na,K- and H,K-ATPase PUBTATOR P-type ATPases Na,K- and H,K-ATPase 399285 The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. 18341291 1 60 gly glycosylation 176:188 arg1 the oligomeric P-type ATPases Na,K- and H,K-ATPase P-type ATPases Na,K- and H,K-ATPase PUBTATOR P-type ATPases Na,K- and H,K-ATPase 399285 The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. 18341291 1 60 gly glycosylation 176:188 arg1 K- P-type ATPases Na,K- and H,K-ATPase PUBTATOR P-type ATPases Na,K- and H,K-ATPase 399285 The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. 18341291 1 60 gly glycosylation 176:188 arg1 the oligomeric P-type ATPases Na,K- and H,K-ATPase P-type ATPases Na,K- and H,K-ATPase PUBTATOR P-type ATPases Na,K- and H,K-ATPase 399285 The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. 18341291 1 60 gly glycosylation 176:188 arg1 K- P-type ATPases Na,K- and H,K-ATPase PUBTATOR P-type ATPases Na,K- and H,K-ATPase 399285 The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. 18341291 1 60 gly glycosylation 176:188 arg1 K- P-type ATPases Na,K- and H,K-ATPase PUBTATOR P-type ATPases Na,K- and H,K-ATPase 399285 The role of N-linked glycosylation of beta-subunits in the functional properties of the oligomeric P-type ATPases Na,K- and H,K-ATPase has been examined by expressing glycosylation-deficient Asn-to-Gln beta-variants in Xenopus oocytes. 28680094 6 69 gly β3-I-EGF3 800:808 arg1 the β3-N654 N-glycan 3 the β3-N654 N-glycan PUBTATOR 3 1934 The β3-N559 N-glycan at the β3-I-EGF3 and αIIb-calf-1 domain interface, and the β3-N654 N-glycan at the β3-β-tail and αIIb-calf-2 domain interface positively regulate the activation of both αIIbβ3 and αVβ3 integrins. 28680094 6 69 gly β3-I-EGF3 800:808 arg1 The β3-N559 N-glycan 3 The β3-N559 N-glycan PUBTATOR 3 1934 The β3-N559 N-glycan at the β3-I-EGF3 and αIIb-calf-1 domain interface, and the β3-N654 N-glycan at the β3-β-tail and αIIb-calf-2 domain interface positively regulate the activation of both αIIbβ3 and αVβ3 integrins. 9083067 6 8 gly contains 689:696 arg1 The OGT protein AND multiple tandem repeats The OGT protein multiple tandem repeats PUBTATOR OGT protein 8473 The OGT protein contains multiple tandem repeats of the tetratricopeptide repeat motif. 9450956 10 53 gly C-glycosylation 1286:1300 arg1 recombinant human interleukin 12 recombinant human interleukin 12 OGER interleukin 12 Two of these proteins were analyzed protein chemically, which showed partial C-glycosylation of recombinant human interleukin 12. 3219367 5 9 gly glycosylation 593:605 arg1 human recombinant erythropoietin human recombinant erythropoietin PUBTATOR erythropoietin 2056 In order to examine the extent to which protein structure influences glycosylation, we have analyzed the saccharide structures at each glycosylation site (Asn24, Asn38, Asn83, and Ser126) of human recombinant erythropoietin. 8870657 10 39 gly unglycosylated 1230:1243 arg1 unglycosylated hLF unglycosylated hLF PUBTATOR hLF 3131 The presence in supernatant of unglycosylated hLF (approx. 27161092 5 78 gly O-glycosylation 786:800 arg1 Muc5ac Muc5ac PUBTATOR Muc5ac 17833 In this study we unraveled the O-glycosylation profile of Muc5ac from glycoengineered mice models lacking the FUT2 enzyme and therefore mimicking a non-secretor human phenotype. 27177499 17 34 gly glycoforms 2377:2386 arg1 hCG hCG OGER hCG Depending on its source of production, glycoforms of hCG display different biological activities and functions that are essential for pregnancy outcome. 1689725 9 2 gly glycoprotein 920:931 arg1 alpha 2HS glycoprotein alpha 2HS glycoprotein PUBTATOR alpha 2HS glycoprotein 280988 No evidence for a separate gene for a bovine alpha 2HS glycoprotein was obtained; thus, fetuin in cattle and alpha 2HS glycoprotein in the human are equivalent proteins. 1689725 9 49 gly glycoprotein 856:867 arg1 a bovine alpha 2HS glycoprotein a bovine alpha 2HS glycoprotein PUBTATOR alpha 2HS glycoprotein 280988 No evidence for a separate gene for a bovine alpha 2HS glycoprotein was obtained; thus, fetuin in cattle and alpha 2HS glycoprotein in the human are equivalent proteins. 3531197 3 50 gly glycoproteins 348:360 arg1 Mac-1 Mac-1 PUBTATOR Mac-1 16409 This cell line simultaneously synthesizes two structurally related glycoproteins, Mac-1 and LFA-1. 3531197 3 50 gly glycoproteins 348:360 arg1 LFA-1 LFA-1 PUBTATOR LFA-1 16414 This cell line simultaneously synthesizes two structurally related glycoproteins, Mac-1 and LFA-1. 23668542 9 15 gly neoglycoprotein 1290:1304 arg1 The A1AT neoglycoprotein The A1AT neoglycoprotein PUBTATOR A1AT neoglycoprotein 5265 The A1AT neoglycoprotein with an additional N-glycosylation site at position N123 exhibited a 62% increase in serum half-life. 11551653 3 93 gly glycoprotein 628:639 arg1 glycoprotein D glycoprotein D OGER glycoprotein D Q16570 The truncated glycoprotein D (gD) gene was placed under the control of the methanol inducible alcohol oxidase 1 promoter and directed for secretion with the Saccharomyces cerevisiae alpha-factor prepro secretion signal. 26106863 10 77 gly glycoprotein 1539:1550 arg1 GP GP Cterm GP Each lineage is defined by multiple mutations, including non-synonymous changes in the virion protein 35 (VP35), glycoprotein (GP) and RNA-dependent RNA polymerase (L) proteins. 10531326 8 6 gly glycosylation 948:960 arg1 apoM apoM PUBTATOR apoM 55937 In vitro translation in the presence of microsomes demonstrated translocation of apoM over the membrane and glycosylation but no signal peptide cleavage. 10603327 3 14 gly sGP 501:503 arg1 oligomannosidic N-glycans sGP oligomannosidic N-glycans Cterm sGP First, the endoplasmic reticulum form sGP(er), full-length sGP with oligomannosidic N-glycans, was detected, sGP(er) was then replaced by the Golgi-specific precursor pre-sGP, full-length sGP containing complex N-glycans. 10603327 3 31 gly containing 634:643 arg1 full-length sGP AND complex N-glycans sGP complex N-glycans Cterm sGP First, the endoplasmic reticulum form sGP(er), full-length sGP with oligomannosidic N-glycans, was detected, sGP(er) was then replaced by the Golgi-specific precursor pre-sGP, full-length sGP containing complex N-glycans. 3718934 9 3 gly glycosylated 1593:1604 arg1 glycosylated calcitonin glycosylated calcitonin OGER calcitonin P01258 These results suggest that in this cell line a minor but significant biosynthetic pathway exists for the production of glycosylated calcitonin from glycosylated procalcitonin. 1420598 2 10 gly glycosylation 275:287 arg1 IL-1 IL-1 PUBTATOR IL-1 3552 To determine the role of glycosylation of the IL-1 receptor type I (IL-1RtI) in the binding and function of IL-1, we used four plant lectins and glycosidase treatment on two different T-cell lines (EL4-6.1 and D10S) and expressing high number of binding sites for IL-1. 21374459 5 18 gly gC-1 1120:1123 arg1 clustered O-linked glycans gC-1 clustered O-linked glycans PUBTATOR gC-1 79751 Use of lectins facilitates a structural analysis of clustered O-linked glycans of gC-1 and it is possible that the methodology presented here may be of more general use, as similar arrangements of clustered O-linked glycans are present in an increasing number known glycoproteins of other enveloped viruses including herpes simplex virus type 2 (12,13), Epstein-Barr virus (14), and respiratory syncytial virus (15). 7961764 1 25 gly glycoprotein 203:214 arg1 The M protein The M protein OGER M protein P54296 The M protein of mouse hepatitis virus (MHV) is a triple-spanning membrane glycoprotein that is exclusively O-glycosylated. 7961764 1 50 gly O-glycosylated 236:249 arg1 The M protein The M protein OGER M protein P54296 The M protein of mouse hepatitis virus (MHV) is a triple-spanning membrane glycoprotein that is exclusively O-glycosylated. 8680440 0 93 gly glycosylated 46:57 arg1 glycosylated rat prolactin glycosylated rat prolactin PUBTATOR prolactin 24683 Identification and localization of 23,000 and glycosylated rat prolactin in subcellular fractions of rat anterior pituitary and purified secretory granules. 22159084 7 19 gly Deglycosylation 821:835 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 Deglycosylation of SLC26A3 causes depression of transport activity compared with wild-type, although robust intracellular pH changes were still observed, suggesting that N-glycosylation is not absolutely necessary for transport activity. 23700425 3 62 gly sOGT 558:561 arg1 the sugar-nucleotide tolerance OGT the sugar-nucleotide tolerance PUBTATOR OGT 8473 In this work, the sugar-nucleotide tolerance of sOGT was investigated. 16716077 3 53 gly nonglycosylated 520:534 arg1 no nonglycosylated sTFR no nonglycosylated sTFR Cterm sTFR 7037 Although fully glycosylated sTFR is secreted into the tissue culture medium ( approximately 40 mg/L), no nonglycosylated sTFR could be produced, suggesting that carbohydrate is critical to the folding, stability, and/or secretion of the receptor. 16716077 3 78 gly glycosylated 430:441 arg1 fully glycosylated sTFR fully glycosylated sTFR Cterm sTFR 7037 Although fully glycosylated sTFR is secreted into the tissue culture medium ( approximately 40 mg/L), no nonglycosylated sTFR could be produced, suggesting that carbohydrate is critical to the folding, stability, and/or secretion of the receptor. 11150304 2 16 gly estrogen 338:345 arg1 O-GlcNAcylated estrogen receptor-beta O-GlcNAcylated OGER estrogen receptor-beta O08537 Recently, we demonstrated that the murine estrogen receptor-beta (mER-beta) is alternatively O-GlcNAcylated or O-phosphorylated at Ser(16). 10369660 4 6 part_of PrP-sen 645:651 arg1 hamster PrP-sen residues 219-232 PrP hamster PrP-sen residues 219-232 OGER SpecificSite PrP P32119 residues 219-232 Binding of antibodies (alpha219-232) to hamster PrP-sen residues 219-232 inhibited the binding of PrP-sen to PrP-res and the subsequent generation of PK-resistant PrP. 2912972 1 7 gly glycoprotein 189:200 arg1 Laminin Laminin PUBTATOR Laminin 38723 Laminin, a major component of basement membranes, is a large glycoprotein consisting of three disulfide-bonded subunits, A, B1, and B2. 7493334 7 62 gly IgG 1490:1492 arg1 the Fc-region carbohydrate IgG the Fc-region carbohydrate Cterm IgG These initial experiments establish that light chain carbohydrate modification of F(ab')2 is as facile as with the Fc-region carbohydrate of intact IgG, and thereby offer the possibility of designing site-specifically substituted F(ab')2 fragments with favorable pharmacokinetic properties. 1280217 4 24 gly derived 725:731 arg1 a single-chain precursor AND The smaller subunit a single-chain precursor The smaller subunit OGER chain precursor 232345 The smaller subunit, with the N-terminal sequence DLSSSDLT, comprises the C-terminal 257 residues of m alpha 2M and is derived from a single-chain precursor probably by proteolytic processing at an arginine residue in the sequence PTRDLSS. 25009769 3 9 gly N-glycosylated 400:413 arg1 HYAL1 HYAL1 OGER HYAL1 Q12794 Using mass spectrometry, we demonstrated that HYAL1 was N-glycosylated at the three asparagine residues. 6651835 3 11 part_of IgM 407:409 arg1 Asn 563 IgM Asn 563 OGER SpecificSite IgM P01872 Asn 563 Amino acid and carbohydrate analyses show that Asn 563 of murine IgM is glycosylated only about 44% of the time. 4075697 1 5 gly glycosylated 185:196 arg1 VLDL VLDL Cterm VLDL Human very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoproteins (HDL2 and HDL3) were glycosylated in vitro by incubation with high concentrations of glucose and glucose-6-phosphate. 4075697 1 5 gly glycosylated 185:196 arg1 LDL LDL Cterm LDL Human very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoproteins (HDL2 and HDL3) were glycosylated in vitro by incubation with high concentrations of glucose and glucose-6-phosphate. 4075697 1 5 gly glycosylated 185:196 arg1 HDL2 HDL2 PUBTATOR HDL2 57338 Human very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoproteins (HDL2 and HDL3) were glycosylated in vitro by incubation with high concentrations of glucose and glucose-6-phosphate. 4075697 1 5 gly glycosylated 185:196 arg1 HDL3 HDL3 PUBTATOR HDL3 53369 Human very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoproteins (HDL2 and HDL3) were glycosylated in vitro by incubation with high concentrations of glucose and glucose-6-phosphate. 11027492 5 79 gly glycosylation 780:792 arg1 11beta-HSD 1 11beta-HSD 1 PUBTATOR 11beta-HSD 1 3290 However, the importance of N-linked glycosylation of 11beta-HSD 1 for catalytic activity has been controversely discussed. 18025088 2 72 gly contain 333:339 arg1 triadin-1 AND N-linked glycans triadin-1 N-linked glycans OGER triadin Q13061 Calsequestrin and triadin-1 both contain N-linked glycans, but about half of triadin-1 in the heart remains unglycosylated. 17960575 8 30 gly N-glycosylation 1155:1169 arg1 TRFE TRFE PUBTATOR TRFE 7018 Disialylated diantennary glycans were observed in glycopeptides of both N-glycosylation sites of TRFE. 17960575 8 35 gly glycopeptides 1133:1145 arg1 TRFE TRFE PUBTATOR TRFE 7018 Disialylated diantennary glycans were observed in glycopeptides of both N-glycosylation sites of TRFE. 11934888 8 31 gly non-glycosylated 1391:1406 arg1 only non-glycosylated ORF2 only non-glycosylated ORF2 PUBTATOR ORF2 1494410 These findings were confirmed using tunicamycin inhibition, point mutants, and deletion mutants expressing only non-glycosylated ORF2. 1850903 0 8 gly glycoprotein 30:41 arg1 glycoprotein G glycoprotein G Cterm HSV-2 glycoprotein G Expression of HSV-1 and HSV-2 glycoprotein G in insect cells by using a novel baculovirus expression vector. 17986444 6 5 gly glycosylation 896:908 arg1 polySia-NCAM polySia-NCAM PUBTATOR polySia-NCAM 17967 Our results revealed an identical glycosylation and almost complete polysialylation of N-glycosylation sites 5 and 6 in polySia-NCAM irrespective of the enzyme present. 17986444 6 91 gly polysialylation 930:944 arg1 polySia-NCAM polySia-NCAM polysialylation PUBTATOR polySia-NCAM 17967 Our results revealed an identical glycosylation and almost complete polysialylation of N-glycosylation sites 5 and 6 in polySia-NCAM irrespective of the enzyme present. 22908222 2 0 gly glycoproteins 297:309 arg1 synaptotagmin 1 synaptotagmin 1 OGER synaptotagmin 1 P21579 For example, the three major synaptic vesicle glycoproteins, synaptotagmin 1, synaptophysin, and SV2, represent ∼30% of the total copy number of vesicle proteins. 22908222 2 0 gly glycoproteins 297:309 arg1 synaptophysin synaptophysin OGER synaptophysin P08247 For example, the three major synaptic vesicle glycoproteins, synaptotagmin 1, synaptophysin, and SV2, represent ∼30% of the total copy number of vesicle proteins. 18952059 4 21 gly glycosylation 461:473 arg1 IZUMO IZUMO PUBTATOR IZUMO 73456 The expression of N204Q-IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO. 9442070 2 52 gly glycosylation 316:328 arg1 IgG IgG Cterm IgG In contrast to IgA1, the glycosylation of IgG has been well characterized, and its interaction with various Fc receptors (Fc Rs) has been well studied. 8737716 9 56 gly microheterogeneity 1827:1844 arg1 alpha 1AT alpha 1AT PUBTATOR alpha 1AT 5265 CONCLUSION: The results suggest that the microheterogeneity of alpha 1AT may play a part in the formation of the IgA-alpha 1AT complex in RA. 10998266 1 31 gly heterogeneity 418:430 arg1 EPO EPO heterogeneity PUBTATOR EPO 2056 High-performance liquid chromatography with electrospray ionization mass spectrometry (LC/MS) and liquid chromatography with tandem mass spectrometry (LC/MS/MS) were applied to the analysis of the site-specific carbohydrate heterogeneity in erythropoietin (EPO) used as a model of the sialylated glycoprotein. 10998266 1 31 gly heterogeneity 418:430 arg1 erythropoietin erythropoietin heterogeneity PUBTATOR erythropoietin 2056 High-performance liquid chromatography with electrospray ionization mass spectrometry (LC/MS) and liquid chromatography with tandem mass spectrometry (LC/MS/MS) were applied to the analysis of the site-specific carbohydrate heterogeneity in erythropoietin (EPO) used as a model of the sialylated glycoprotein. 8494607 5 17 gly N-glycosylation 773:787 arg1 mature renin mature renin PUBTATOR renin 5972 In the present study, the role of N-glycosylation in the secretion of mature renin was examined. 2007624 0 109 gly CD44 34:37 arg1 a heparan-sulfate intrinsic membrane proteoglycan CD44 a heparan-sulfate intrinsic membrane proteoglycan PUBTATOR CD44 960 Human keratinocytes express a new CD44 core protein (CD44E) as a heparan-sulfate intrinsic membrane proteoglycan with additional exons. 15474003 7 70 gly N-glycosylation 1083:1097 arg1 the CaValpha2delta subunit-induced current stimulation the CaValpha2delta subunit-induced current stimulation Cterm CaValpha2delta These results corroborate that N-glycosylation is required for the CaValpha2delta subunit-induced current stimulation and suggest that sites N136 and N184 are directly involved in this action. 23014585 0 72 gly glycosylation 39:51 arg1 P-selectin P-selectin OGER P-selectin P16109 The Thr715Pro variant impairs terminal glycosylation of P-selectin. 10773191 1 24 gly glycoprotein 164:175 arg1 Tenascin-R Tenascin-R OGER Tenascin Q80YX1 Tenascin-R (TN-R), a matrix glycoprotein of the central nervous system (CNS), has been implicated in a variety of cell-matrix interactions involved in the control of axon growth, myelination and cell adhesion to fibronectin during development and regeneration. 18339697 5 48 gly deglycosylated 789:802 arg1 deglycosylated NT-proBNP deglycosylated NT-proBNP PUBTATOR BNP 4879 RESULTS: Treatment of endogenous NT-proBNP by a mixture of glycosidases resulted in significant improvement of the interaction between deglycosylated NT-proBNP and monoclonal antibodies (MAbs) specific to the mid-fragment of the molecule. 10559353 6 11 gly deglycosylation 1243:1257 arg1 CCR5 CCR5 PUBTATOR CCR5 1234 Binding was not significantly affected by the pH of binding, G-protein coupling of CCR5, or partial gp120 deglycosylation. 22487187 0 78 gly N-glycosylation 108:122 arg1 HSP60 HSP60 OGER HSP60 Q0VDF9 HSP60 is transported through the secretory pathway of 3-MCA-induced fibrosarcoma tumour cells and undergoes N-glycosylation. 21385452 6 59 gly glycoforms 1016:1025 arg1 MUC1 MUC1 PUBTATOR MUC1 4582 METHODS: We used a microarray platform of 60mer MUC1 glycopeptides, to confirm the presence of autoantibodies to cancer associated glycoforms of MUC1 in a proportion of early breast cancer patients (54/198). 9207473 8 94 gly presence 1615:1622 arg2 recombinant human calreticulin AND carbohydrate recombinant human calreticulin carbohydrate PUBTATOR calreticulin 811 Direct measurement by phenol-H2SO4 confirmed the presence of carbohydrate on recombinant human calreticulin. 9719151 4 62 gly O-glycosylation 660:674 arg1 serum IgA1 serum IgA1 PUBTATOR IgA1 3493 METHODS: The O-glycosylation of serum IgA1 from a series of patients with IgAN and matched controls was assessed by lectin binding assay. 24361716 8 21 gly glycosylation 1418:1430 arg1 ADAM17 ADAM17 PUBTATOR ADAM17 6868 These results suggest that glycosylation of ADAM17 can affect cell signaling in disease and might provide opportunities for therapeutic intervention using exosite inhibitors. 18322210 1 60 gly glycosylation 164:176 arg1 HIV-1 envelope gp120 HIV-1 envelope gp120 PUBTATOR gp120 155971 The heavy glycosylation of HIV-1 envelope gp120 shields this important Ag from recognition by neutralizing Abs and cytolytic CD8 T cells. 19050247 6 25 gly IgG1 1190:1193 arg1 oligosaccharide chains IgG1 oligosaccharide chains Cterm IgG1 Differences in the glycan composition were observed when we analyzed oligosaccharide chains from anaphylactic or non-anaphylactic IgG1, mainly the presence of more sialic acid and fucose residues in anaphylactic molecules. 7774058 4 48 gly IgG 734:736 arg1 the N-linked carbohydrate chains IgG the N-linked carbohydrate chains Cterm IgG Lectin binding assays were designed to examine the expression of terminal galactose on the N-linked carbohydrate chains of purified serum IgG and IgA1, and the O-linked sugars of IgA1 and C1 inhibitor (one of the very few other serum proteins with O-linked glycosylation). 7774058 4 48 gly IgG 734:736 arg1 the O-linked sugars IgG the O-linked sugars Cterm IgG Lectin binding assays were designed to examine the expression of terminal galactose on the N-linked carbohydrate chains of purified serum IgG and IgA1, and the O-linked sugars of IgA1 and C1 inhibitor (one of the very few other serum proteins with O-linked glycosylation). 7774058 4 52 gly IgA1 742:745 arg1 the N-linked carbohydrate chains IgA1 the N-linked carbohydrate chains PUBTATOR IgA1 3493 Lectin binding assays were designed to examine the expression of terminal galactose on the N-linked carbohydrate chains of purified serum IgG and IgA1, and the O-linked sugars of IgA1 and C1 inhibitor (one of the very few other serum proteins with O-linked glycosylation). 7774058 4 52 gly IgA1 742:745 arg1 the O-linked sugars IgA1 the O-linked sugars PUBTATOR IgA1 3493 Lectin binding assays were designed to examine the expression of terminal galactose on the N-linked carbohydrate chains of purified serum IgG and IgA1, and the O-linked sugars of IgA1 and C1 inhibitor (one of the very few other serum proteins with O-linked glycosylation). 7774058 4 61 gly IgA1 775:778 arg1 the N-linked carbohydrate chains IgA1 the N-linked carbohydrate chains PUBTATOR IgA1 3493 Lectin binding assays were designed to examine the expression of terminal galactose on the N-linked carbohydrate chains of purified serum IgG and IgA1, and the O-linked sugars of IgA1 and C1 inhibitor (one of the very few other serum proteins with O-linked glycosylation). 7774058 4 61 gly IgA1 775:778 arg1 the O-linked sugars IgA1 the O-linked sugars PUBTATOR IgA1 3493 Lectin binding assays were designed to examine the expression of terminal galactose on the N-linked carbohydrate chains of purified serum IgG and IgA1, and the O-linked sugars of IgA1 and C1 inhibitor (one of the very few other serum proteins with O-linked glycosylation). 14527339 3 43 gly non-glycosylated 610:625 arg1 non-glycosylated [Syn2 non-glycosylated [Syn2 PUBTATOR Syn2 6854 The glycosylated [Syn2(ect)(+HS)] and non-glycosylated [Syn2(ect)(-HS)] forms of Syn2(ect) (the syndecan-2 ectodomain) were purified from a stably transfected human cell line and from a bacterial expression system respectively. 14527339 3 50 gly glycosylated 576:587 arg1 Syn2 Syn2 PUBTATOR Syn2 6854 The glycosylated [Syn2(ect)(+HS)] and non-glycosylated [Syn2(ect)(-HS)] forms of Syn2(ect) (the syndecan-2 ectodomain) were purified from a stably transfected human cell line and from a bacterial expression system respectively. 9757569 0 10 part_of residue 32:38 arg1 ovine angiotensinogen angiotensinogen residue PUBTATOR AminoAcid angiotensinogen 183 residue at position 14 Effects of glycosylation of the residue at position 14 in ovine angiotensinogen on the human renin reaction. 3817304 6 69 gly nonglycosylated 1137:1151 arg1 glycosylated and nonglycosylated albumin glycosylated and nonglycosylated albumin OGER albumin P02768 Incubation of solutions of glycosylated and nonglycosylated albumin demonstrated significantly lower binding to the glycosylated fraction (P = 8.1 X 10(-6)). 3817304 6 72 gly glycosylated 1120:1131 arg1 glycosylated and nonglycosylated albumin glycosylated and nonglycosylated albumin OGER albumin P02768 Incubation of solutions of glycosylated and nonglycosylated albumin demonstrated significantly lower binding to the glycosylated fraction (P = 8.1 X 10(-6)). 20097169 4 4 gly glycosylated 611:622 arg1 a functionally inactive, endoplasmic reticulum-retained and partially glycosylated BACE a functionally inactive, endoplasmic reticulum-retained and partially glycosylated BACE PUBTATOR BACE 23621 In contrast, substituting both active site aspartic acid residues produced a functionally inactive, endoplasmic reticulum-retained and partially glycosylated BACE. 10861210 5 15 part_of AE1 819:821 arg1 N555 AE1 N555 PUBTATOR SpecificSite AE1 6521 N555 Moving the N-glycosylation site to the preceding extracellular loop in an AE1 glycosylation mutant (N555) resulted in processing of the oligosaccharide and production of a complex form of AE1. 19579232 4 65 gly glycosylated 836:847 arg1 glycosylated 24 kDa hGH glycosylated 24 kDa hGH Cterm hGH 2688 After beta-elimination to release the oligosaccharide from glycosylated 24 kDa hGH, collision-induced dissociation of tryptic glycopeptide T6 indicated that there had been an O-linked oligosaccharide attached to Thr-60. 8416385 5 69 gly glycosylated 707:718 arg1 glycosylated gp120 glycosylated gp120 PUBTATOR gp120 155971 Enzymatic removal of carbohydrate chains from glycosylated gp120 by endoglycosidase H or an endoglycosidase F/N glycanase mixture had no effect on the ability of gp120 to bind CD4. 12584318 8 67 gly contains 2062:2069 arg1 hamster ASCT1 AND an additional N-linked oligosaccharide hamster ASCT1 an additional N-linked oligosaccharide PUBTATOR ASCT1 55963 In contrast to mASCT1, which contains two N-linked oligosaccharides that partially restrict viral infections, hamster ASCT1 contains an additional N-linked oligosaccharide clustered close to the others in the carboxyl-terminal region of ECL2. 19822741 4 11 gly structures 699:708 arg1 HA HA structures Cterm HA Truncation of the N-glycan structures on HA increased SA binding affinities while decreasing specificity toward disparate SA ligands. 1897978 9 54 gly hGM-CSF 1600:1606 arg1 N-linked carbohydrate moieties hGM-CSF N-linked carbohydrate moieties PUBTATOR hGM-CSF 1437 From these findings, we concluded that N-linked carbohydrate moieties of hGM-CSF play conflicting physiological roles in the efficacy of the protein in vivo but that O-linked carbohydrate moieties do not have such effects. 10213617 1 26 gly P-glycoprotein 245:258 arg1 P-gp P-gp PUBTATOR P-gp 5243 Multiple topologies have been detected for the COOH-terminal half of the human multidrug resistance P-glycoprotein (P-gp). 10213617 1 26 gly P-glycoprotein 245:258 arg1 the human multidrug resistance P-glycoprotein the human multidrug resistance P-glycoprotein PUBTATOR P-glycoprotein 5243 Multiple topologies have been detected for the COOH-terminal half of the human multidrug resistance P-glycoprotein (P-gp). 9514971 1 33 gly glycosylation 142:154 arg1 gp41 gp41 Cterm gp41 The requirement for glycosylation in the transmembrane protein, gp41, of human immunodeficiency virus type 1 envelope protein for fusion activity has been studied. 3402460 11 32 gly AGP-C 2028:2032 arg1 the biantennary glycans AGP the biantennary glycans Cterm AGP It was shown that about 90% of the biantennary glycans of both AGP-B and AGP-C were disialylated while the remainder were monosialylated. 3402460 11 45 gly AGP-B 2018:2022 arg1 the biantennary glycans AGP-B the biantennary glycans PUBTATOR AGP-B 5005 It was shown that about 90% of the biantennary glycans of both AGP-B and AGP-C were disialylated while the remainder were monosialylated. 14764083 0 50 gly glycosylation 21:33 arg1 human and bovine lactoferrin human and bovine lactoferrin PUBTATOR lactoferrin 280846 The role of N-linked glycosylation in the protection of human and bovine lactoferrin against tryptic proteolysis. 25505062 4 77 gly glycosylated 585:596 arg1 glycosylated Pr80 Gag glycosylated Pr80 Gag PUBTATOR Pr80 Gag 17276 Glycosylated Gag, also called glycosylated Pr80 Gag (gPr80), is a gammaretrovirus genome-encoded protein that inhibits the antiretroviral activity of mouse A3 (mA3). 25505062 4 77 gly glycosylated 585:596 arg1 gPr80 gPr80 OGER gPr80 Q6IYF8 Glycosylated Gag, also called glycosylated Pr80 Gag (gPr80), is a gammaretrovirus genome-encoded protein that inhibits the antiretroviral activity of mouse A3 (mA3). 18187336 3 45 gly glycosylated 545:556 arg1 E1 E1 and E2 PUBTATOR E1 and E2 6080 The two envelope proteins E1 and E2 are heavily glycosylated. 9202988 4 34 gly glycosylation 495:507 arg1 serum FSH serum FSH OGER FSH The aim of the present investigation was to study bioactivity and the glycosylation pattern of serum FSH and the occurrence of mutations in the FSH receptor in infertile patients with SCO compared to normal men. 9140197 1 8 gly glycoprotein 208:219 arg1 glycoprotein 2 glycoprotein 2 PUBTATOR glycoprotein 2 2813 Two monoclonal antibody escape virus mutants (MARs), rescued from a human MAb to glycoprotein 2 (G2) and a bank vole monoclonal antibody (MAb) directed to glycoprotein 1 (G1) of Puumala virus, strain Sotkamo, were produced by using a combination of neutralization tests and antigen detection. 9140197 1 8 gly glycoprotein 208:219 arg1 G2 G2 PUBTATOR 2 (G2 2813 Two monoclonal antibody escape virus mutants (MARs), rescued from a human MAb to glycoprotein 2 (G2) and a bank vole monoclonal antibody (MAb) directed to glycoprotein 1 (G1) of Puumala virus, strain Sotkamo, were produced by using a combination of neutralization tests and antigen detection. 9140197 1 19 gly glycoprotein 282:293 arg1 glycoprotein 1 glycoprotein 1 PUBTATOR glycoprotein 1 5544 Two monoclonal antibody escape virus mutants (MARs), rescued from a human MAb to glycoprotein 2 (G2) and a bank vole monoclonal antibody (MAb) directed to glycoprotein 1 (G1) of Puumala virus, strain Sotkamo, were produced by using a combination of neutralization tests and antigen detection. 9140197 1 19 gly glycoprotein 282:293 arg1 G1 G1 Cterm G1 5544 Two monoclonal antibody escape virus mutants (MARs), rescued from a human MAb to glycoprotein 2 (G2) and a bank vole monoclonal antibody (MAb) directed to glycoprotein 1 (G1) of Puumala virus, strain Sotkamo, were produced by using a combination of neutralization tests and antigen detection. 17634239 0 24 gly modifications 16:28 arg1 gp120 gp120 modifications PUBTATOR gp120 155971 N-linked glycan modifications in gp120 of human immunodeficiency virus type 1 subtype C render partial sensitivity to 2G12 antibody neutralization. 26862918 5 19 part_of position 875:882 arg1 the HA HA position Cterm SpecificSite HA asparagine residue at position 141 Glycosylation of the asparagine residue at position 141 (N141) (N133, H3 HA numbering) in the HA of A/Netherlands/219/2003 HA is responsible for this resistance, and it affects the infectivity of HA-pseudoviruses. 26862918 5 68 part_of HA 905:906 arg1 position 141 H3 HA position 141 Cterm SpecificSite H3 HA asparagine residue at position 141 Glycosylation of the asparagine residue at position 141 (N141) (N133, H3 HA numbering) in the HA of A/Netherlands/219/2003 HA is responsible for this resistance, and it affects the infectivity of HA-pseudoviruses. 27773703 1 20 gly glycoprotein 115:126 arg1 Megalin Megalin PUBTATOR Megalin 14725 Megalin is a 600-kDa single-spanning transmembrane glycoprotein and functions as an endocytic receptor, distributed not only in the kidney but also in other tissues. 8810291 4 49 gly N-glycosylation 826:840 arg1 F-S-P F-S-P Cterm F-S-P 5126 F-S-P, F-N-P, and F-S were catalytically active and underwent post-translational proteolysis and N-glycosylation with similar kinetics to wild-type furin. 8521372 7 125 gly glycoforms 1445:1454 arg1 the MUC1 mucin the MUC1 mucin PUBTATOR MUC1 mucin 4582 Of particular interest was the very different spectrum of reactivity observed with the CASA and BC2SSAM assays which use the same capture mab, indicating that each assay detects different glycoforms of the MUC1 mucin. 1705175 0 72 gly deglycosylated 51:64 arg1 deglycosylated ricin A chain deglycosylated ricin A chain OGER chain 2 Covalent binding of human alpha 2-macroglobulin to deglycosylated ricin A chain and its immunotoxins. 18686987 1 29 gly Protein 333:339 arg1 the three major allele products Vitamin D Binding Protein the three major allele products PUBTATOR Vitamin D Binding Protein 2638 Mass spectrometric evidence presented here characterizes the genotype-dependent glycosylation patterns for each of the three major allele products of Vitamin D Binding Protein found in the general human population. 25661536 0 27 gly glycoforms 67:76 arg1 recombinant hFSH glycoforms recombinant hFSH glycoforms Cterm hFSH Production, purification, and characterization of recombinant hFSH glycoforms for functional studies. 1381541 3 20 gly chains 579:584 arg1 E1 E1 chains Cterm E1 Expressed E1 glycosylation mutant proteins were recognized by a panel of E1-specific monoclonal antibodies in radioimmunoprecipitation, immunofluorescence, and immunoblotting, indicating that carbohydrate side chains on E1 are not involved in the constitution of epitopes recognized by these monoclonal antibodies. 7690959 0 107 gly glycoprotein 15:26 arg1 Human platelet glycoprotein Human platelet glycoprotein PUBTATOR platelet glycoprotein V 2814 Human platelet glycoprotein V: characterization of the polypeptide and the related Ib-V-IX receptor system of adhesive, leucine-rich glycoproteins. 2460458 1 12 gly contains 188:195 arg1 The beta subunit AND two asparagine (N)-linked oligosaccharides The beta subunit two asparagine (N)-linked oligosaccharides OGER subunit P0DN86 The beta subunit of human chorionic gonadotropin contains two asparagine (N)-linked oligosaccharides. 19671700 7 71 gly TSR1 1247:1250 arg1 C-Mannosylation TSR1 C-Mannosylation PUBTATOR TSR1 100767392 C-Mannosylation of TSR1 of the related protease ADAMTS5 was also identified. 11485549 2 58 gly beta-1,2-N-acetylglucosaminyltransferase 349:388 arg1 GlcNAc-TI N-acetylglucosaminyltransferase I GlcNAc-TI OGER N-acetylglucosaminyltransferase I Q8N0V5 To investigate if the medial-Golgi enzyme beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-TI) is transported to the late Golgi, a modified GlcNAc-TI bearing an N-glycan site on the C-terminus was constructed. 8889826 1 84 gly IgA1 349:352 arg1 the O-linked oligosaccharide IgA1 the O-linked oligosaccharide PUBTATOR IgA1 3493 In our previous study, gas-phase hydrazinolysis was used to analyze the glycoform of the O-linked oligosaccharide of human serum IgA1. 17975018 0 41 gly glycosylation 9:21 arg1 VWF VWF PUBTATOR VWF 7450 N-linked glycosylation of VWF modulates its interaction with ADAMTS13. 12175915 7 66 gly deglycosylated 1516:1529 arg1 deglycosylated RFC-Gln deglycosylated RFC-Gln PUBTATOR SpecificSite RFC 6573 Insertion of a consensus N-glycosylation site [NX(S/T)] into putative loops 5/6, 8/9, and 9/10 of deglycosylated RFC-Gln(58) had minimal effects on MTX transport. 12954207 9 28 gly glycoprotein 1537:1548 arg1 Envelope glycoprotein oligomers Envelope glycoprotein oligomers PUBTATOR Envelope glycoprotein 100616444 Envelope glycoprotein oligomers on the cell surface derived from the V3 glycan-deficient virus were better recognized by a CD4BS antibody and a V3 loop antibody than were the wild-type glycoproteins. 22191536 1 49 gly glycoprotein 153:164 arg1 The folate binding protein The folate binding protein PUBTATOR folate binding protein 2348 The folate binding protein (FBP), also known as the folate receptor (FR), is a glycoprotein which binds the vitamin folic acid and its analogues. 1748298 8 44 gly glycosylated 1226:1237 arg1 K. lactis reIL-1 beta K. lactis reIL-1 beta PUBTATOR IL-1 beta 3553 As in Saccharomyces cerevisiae [Baldari et al., EMBO J. 6 (1987) 229-234], but unlike native human IL-1 beta, K. lactis reIL-1 beta is glycosylated. 2556847 3 1 gly glycosylation 368:380 arg1 gp65 gp65 PUBTATOR gp65 27020 The glycosylation of gp65 in virus-infected cells was inhibited by tunicamycin but not by monensin, suggesting that it contains an N-glycosidic linkage. 8407981 4 34 gly glycosylated 500:511 arg1 The P-450(arom) protein The P-450(arom) protein PUBTATOR P-450(arom) protein 55010 The P-450(arom) protein expressed in the insect cells was glycosylated, and the sugar chain was sensitive to Endo H. 22868230 7 23 gly mGASP-1 1192:1198 arg1 the carbohydrate moiety mGASP-1 the carbohydrate moiety PUBTATOR mGASP-1 278507 CONCLUSION: Analysis of structure-function relationships of murine GASP-1 provides insights into the involvement of the carbohydrate moiety of mGASP-1 on its biological activity. 26968544 1 49 gly dystrophin-glycoprotein 198:220 arg1 dystrophin-glycoprotein dystrophin-glycoprotein OGER dystrophin P11530 Delta-sarcoglycan is a component of the sarcoglycan subcomplex within the dystrophin-glycoprotein complex located at the plasma membrane of muscle cells. 20107545 2 40 gly non-glycosylated 450:465 arg1 non-glycosylated Tim-3 non-glycosylated Tim-3 PUBTATOR Tim-3 171285 Recently, it has been shown that N-glycosylation affects the binding activity of the Tim-3-Ig fusion protein to its ligand, galectin-9, but the binding properties of non-glycosylated Tim-3 on CD4(+)CD25(+) T cells has not been fully examined. 10970800 10 73 gly glycosylation 1381:1393 arg1 the EP3beta receptor the EP3beta receptor PUBTATOR EP3beta receptor 64184 Therefore, glycosylation of the EP3beta receptor seems not to be necessary for correct folding of the receptor protein but for the efficient transport of the receptor protein to the plasma membrane. 23202458 3 44 gly glycoprotein 456:467 arg1 the envelope glycoprotein GP the envelope glycoprotein GP PUBTATOR envelope glycoprotein GP 64006 Arenaviruses are comprised of two RNA genome segments and four proteins, the polymerase L, the envelope glycoprotein GP, the matrix protein Z, and the nucleoprotein NP. 6433977 4 56 gly IgG 685:687 arg1 the Asn-297 oligosaccharides IgG the Asn-297 oligosaccharides Cterm IgG Because of the unusual nature of these structures, the Asn-297 oligosaccharides of the same IgG were prepared from Fc fragments and heavy chains. 8323299 4 58 gly glycosylated 619:630 arg1 lamp-1 lamp-1 PUBTATOR In lamp-1 3916 In lamp-1, Thr-171, Thr-172, Ser-179, Ser-181, and Ser-183 were fully glycosylated, whereas Ser-169 was partially glycosylated. 25485983 4 16 gly glycosylation 615:627 arg1 antithrombin antithrombin PUBTATOR antithrombin 462 We evaluated the effect of the aromatic sequon in this defective glycosylation site of antithrombin, despite of being located in a loop between the helix D and the strand 2A. 7780197 1 21 gly contains 140:147 arg1 TfR AND three N-linked oligosaccharides TfR three N-linked oligosaccharides PUBTATOR TfR 7037 The human transferrin receptor (TfR) contains three N-linked oligosaccharides and glycosylation is required for the proper folding and function of the molecule. 7780197 1 21 gly contains 140:147 arg1 The human transferrin receptor AND three N-linked oligosaccharides The human transferrin receptor three N-linked oligosaccharides PUBTATOR transferrin receptor 7037 The human transferrin receptor (TfR) contains three N-linked oligosaccharides and glycosylation is required for the proper folding and function of the molecule. 17095532 4 26 gly deglycosylated 755:768 arg1 deglycosylated Cit s 1 deglycosylated Cit s 1 PUBTATOR Cit s 1 11113 These reagents also inhibited the interaction of Cit s 1 with patients' sera, thus underlining the critical role of glycosylation in the recognition of this protein by patients' IgE and extending previous data showing that deglycosylated Cit s 1 does not possess IgE epitopes. 9578495 1 7 gly choriogonadotropin 158:175 arg1 the N-linked oligosaccharide choriogonadotropin the N-linked oligosaccharide OGER choriogonadotropin Several studies indicate that in human choriogonadotropin the N-linked oligosaccharide at position 52 of the alpha-subunit is important for bioactivity. 20826563 9 4 gly desialylated 1210:1221 arg1 desialylated 3N-GH desialylated 3N-GH Cterm 3N-GH The terminal half-life of 3N-GH after iv injection was 24-fold prolonged compared with wild-type GH for the pool with the most pronounced sialylation, 13-fold prolonged for the less sialylated pool, and similar to the wild-type for desialylated 3N-GH. 16627478 10 32 gly glycosylation 1411:1423 arg1 MT1-MMP MT1-MMP PUBTATOR MT1-MMP 4323 Overall, our results point out that there is a delicate balance between glycosylation and self-proteolysis of MT1-MMP in cancer cells and that when this balance is upset the catalytically potent MT1-MMP pool is self-proteolyzed. 30110893 3 58 part_of F1 639:640 arg1 N500 F1 N500 Cterm SpecificSite F1 N500 The protein conserves 5 N-glycosylation sites, two of which are located in the F2 subunit (N27 and N70), one in the F1 subunit (N500) and two in the p27 peptide (N116 and N126). 7852411 6 15 gly N-glycosylation 1263:1277 arg1 tPA tPA OGER tPA P00750 In the presence of fibrinogen fragments, N-glycosylation of plasminogen at site 289 modulates the kinetics of association of enzyme and substrate, while N-glycosylation at site 184 on tPA modulates the turnover rate of the enzyme. 7852411 6 53 gly N-glycosylation 1151:1165 arg1 plasminogen plasminogen OGER plasminogen P00747 In the presence of fibrinogen fragments, N-glycosylation of plasminogen at site 289 modulates the kinetics of association of enzyme and substrate, while N-glycosylation at site 184 on tPA modulates the turnover rate of the enzyme. 26572623 5 5 gly glycoproteins 726:738 arg1 EMC1 EMC1 OGER EMC1 Q8N766 We found that native but unstable or somewhat unfolded glycoproteins, such as ATF6α, ATF6α(C), CD3-δ-ΔTM, and EMC1, were stabilized in EDEM1/2/3 triple knockout cells. 26572623 5 5 gly glycoproteins 726:738 arg1 ATF6α ATF6 OGER ATF6 P18850 We found that native but unstable or somewhat unfolded glycoproteins, such as ATF6α, ATF6α(C), CD3-δ-ΔTM, and EMC1, were stabilized in EDEM1/2/3 triple knockout cells. 12738639 0 10 gly glycosylation 66:78 arg1 the toll-like receptor 4 protein the toll-like receptor 4 protein PUBTATOR toll-like receptor 4 protein 7099 MD-2 is necessary for the toll-like receptor 4 protein to undergo glycosylation essential for its translocation to the cell surface. 7492680 1 2 gly glycoprotein 160:171 arg1 MOGP MOGP PUBTATOR MOGP 12659 In the present study, we have isolated the cDNA for the mouse oviduct-specific glycoprotein (MOGP) by screening the mouse oviduct cDNA library with the bovine oviduct-specific glycoprotein (BOGP)-cDNA probe and by the 5' rapid amplification of the cDNA end (5'RACE). 7492680 1 2 gly glycoprotein 160:171 arg1 the mouse oviduct-specific glycoprotein the mouse oviduct-specific glycoprotein PUBTATOR oviduct-specific glycoprotein 12659 In the present study, we have isolated the cDNA for the mouse oviduct-specific glycoprotein (MOGP) by screening the mouse oviduct cDNA library with the bovine oviduct-specific glycoprotein (BOGP)-cDNA probe and by the 5' rapid amplification of the cDNA end (5'RACE). 7492680 1 42 gly glycoprotein 257:268 arg1 BOGP BOGP Cterm BOGP 280886 In the present study, we have isolated the cDNA for the mouse oviduct-specific glycoprotein (MOGP) by screening the mouse oviduct cDNA library with the bovine oviduct-specific glycoprotein (BOGP)-cDNA probe and by the 5' rapid amplification of the cDNA end (5'RACE). 7492680 1 42 gly glycoprotein 257:268 arg1 the bovine oviduct-specific glycoprotein the bovine oviduct-specific glycoprotein PUBTATOR oviduct-specific glycoprotein 280886 In the present study, we have isolated the cDNA for the mouse oviduct-specific glycoprotein (MOGP) by screening the mouse oviduct cDNA library with the bovine oviduct-specific glycoprotein (BOGP)-cDNA probe and by the 5' rapid amplification of the cDNA end (5'RACE). 12888867 7 61 gly non-glycosylated 943:958 arg1 PAI-1 PAI-1 PUBTATOR PAI-1 5054 The different biochemical properties of glycosylated and non-glycosylated PAI-1 depended specifically on glycosylation of either one or the other of the utilised sites. 20512925 7 3 gly Glycosylation 928:940 arg1 RAGE RAGE PUBTATOR RAGE 177 Glycosylation of RAGE and maximum binding sites for S100A12 on RAGE are also cell type dependent. 2713370 1 25 gly glycosylation 198:210 arg1 secreted monoclonal IgG secreted monoclonal IgG Cterm IgG A panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal IgG. 24213971 4 6 part_of subunit 633:639 arg1 N104 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 6 part_of subunit 633:639 arg1 N32 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 6 part_of subunit 633:639 arg1 the three β2 subunit glycosylation sites 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 6 part_of subunit 633:639 arg1 N32 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 6 part_of subunit 633:639 arg1 the three β2 subunit glycosylation sites 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 6 part_of subunit 633:639 arg1 the three β2 subunit glycosylation sites 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 68 part_of β2 630:631 arg1 N104 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 68 part_of β2 630:631 arg1 N32 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 68 part_of β2 630:631 arg1 the three β2 subunit glycosylation sites 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 68 part_of β2 630:631 arg1 N32 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 68 part_of β2 630:631 arg1 the three β2 subunit glycosylation sites 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24213971 4 68 part_of β2 630:631 arg1 the three β2 subunit glycosylation sites 2 subunit sites, N32, N104 and N173 PUBTATOR SpecificSite 2 subunit 15130 sites, N32, N104 and N173 We reported previously that glycosylation of the three β2 subunit glycosylation sites, N32, N104 and N173, was important for α1β2 receptor channel gating. 24465884 1 101 gly glycoprotein 244:255 arg1 Env Env PUBTATOR Env 155971 The importance of the fourth variable (V4) region of the human immunodeficiency virus 1 (HIV-1) envelope glycoprotein (Env) in virus infection has not been well clarified, though the polymorphism of this region has been found to be associated with disease progression to acquired immunodeficiency syndrome (AIDS). 24465884 1 101 gly glycoprotein 244:255 arg1 the human immunodeficiency virus 1 (HIV-1) envelope glycoprotein the human immunodeficiency virus 1 (HIV-1) envelope glycoprotein PUBTATOR HIV-1) envelope glycoprotein 155971 The importance of the fourth variable (V4) region of the human immunodeficiency virus 1 (HIV-1) envelope glycoprotein (Env) in virus infection has not been well clarified, though the polymorphism of this region has been found to be associated with disease progression to acquired immunodeficiency syndrome (AIDS). 29501745 2 8 gly C-mannosylated 386:399 arg1 G-CSFR G-CSFR PUBTATOR G-CSFR 12986 G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. 29501745 2 1 gly has 272:274 arg1 G-CSFR AND three putative C-mannosylation sites G-CSFR three putative C-mannosylation sites PUBTATOR G-CSFR 12986 G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. 3360214 12 50 gly glycosylated 1788:1799 arg1 laminin laminin OGER laminin [3H]heparin binding at a fixed concentration to a constant amount of control or 12-day nonenzymatically glycosylated laminin and type IV collagen was also studied. 18623533 0 42 gly interferon-gamma 31:46 arg1 N-glycans interferon-gamma N-glycans PUBTATOR interferon-gamma 3458 N-glycans of recombinant human interferon-gamma change during batch culture of chinese hamster ovary cells. 12270132 2 42 gly glycosylated 366:377 arg1 the beta(1)AR the beta(1)AR PUBTATOR beta(1)AR 153 We show here that the beta(1)AR is glycosylated in various cell types and that mutation of the single predicted site of N-linked glycosylation (N15A) results in the formation of receptors that are not N-glycosylated. 11171070 12 45 gly deglycosylated 1279:1292 arg1 SFT3 SFT3 Cterm SFT3 2525 SFT3 secreted by Sf9 cells was completely deglycosylated by peptide-N-glycanase F, whereas 50% of SFT3 secreted by Tn cells was resistant to deglycosylation by this enzyme. 25244057 8 96 gly glycosylation 1571:1583 arg1 fibronectin fibronectin PUBTATOR fibronectin 2335 Our glycoproteomics approach together with the concurrent use of an antibody and lectin is applicable to the quantitative and qualitative monitoring of variations in glycosylation of fibronectin specific to certain types of lung cancer tissue. 2346009 0 16 gly glycosylation 14:26 arg1 serum albumin serum albumin PUBTATOR serum albumin 213 [Nonenzymatic glycosylation of serum albumin and thymic DNA by food monosugars and their natural metabolites that form in the body]. 26884342 4 47 gly polysialylated 784:797 arg1 membrane-associated neuropilin-1 membrane-associated neuropilin-1 PUBTATOR neuropilin-1 8829 To our surprise, we found that membrane-associated neuropilin-1 is polysialylated at ∼50% of the level of neuropilin-2 but not polysialylated when it lacks its cytoplasmic tail and transmembrane region and is secreted from the cell. 26884342 4 53 gly polysialylated 724:737 arg1 membrane-associated neuropilin-1 membrane-associated neuropilin-1 PUBTATOR neuropilin-1 8829 To our surprise, we found that membrane-associated neuropilin-1 is polysialylated at ∼50% of the level of neuropilin-2 but not polysialylated when it lacks its cytoplasmic tail and transmembrane region and is secreted from the cell. 11689624 14 18 gly sites 2396:2400 arg1 gp41 gp41 sites Cterm gp41 Given their high degree of conservation, the four N-linked carbohydrate attachment sites on the external domain of gp41 are surprisingly dispensable for viral replication. 16083266 8 86 gly glycosylated 2417:2428 arg1 beta-casein beta-casein OGER beta-casein P05814 Using ERPA, we demonstrate >95% sequence coverage in the analysis of two heavily phosphorylated and glycosylated proteins, beta-casein at the 50 fmole level and the epidermal growth factor receptor (EGFR) at the 1 pmole level. 16083266 8 86 gly glycosylated 2417:2428 arg1 the epidermal growth factor receptor the epidermal growth factor receptor PUBTATOR epidermal growth factor receptor 1956 Using ERPA, we demonstrate >95% sequence coverage in the analysis of two heavily phosphorylated and glycosylated proteins, beta-casein at the 50 fmole level and the epidermal growth factor receptor (EGFR) at the 1 pmole level. 10415045 1 6 gly glycoform 182:190 arg1 murine CD43 murine CD43 PUBTATOR CD43 20737 The mAb 1B11 has been characterized as recognizing the activation-associated glycoform of murine CD43, a heavily O-glycosylated protein implicated in leukocyte homing. 3123215 1 56 gly glycoprotein 115:126 arg1 Synaptophysin Synaptophysin PUBTATOR Synaptophysin 24804 Synaptophysin is a major glycoprotein of Mr approximately 38,000 (in deglycosylated form: Mr approximately 34,000) characteristic of a certain class of small (30-80 nm diameter) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. 22908222 4 3 gly sites 653:657 arg1 synaptotagmin 1 synaptotagmin 1 sites OGER synaptotagmin 1 P21579 In this study, we analyzed all glycosylation sites on synaptotagmin 1, synaptophysin, and SV2A via mutagenesis and optical imaging of pHluorin-tagged proteins in cultured neurons from knock-out mice lacking each protein. 22908222 4 3 gly sites 653:657 arg1 synaptophysin synaptophysin sites OGER synaptophysin P08247 In this study, we analyzed all glycosylation sites on synaptotagmin 1, synaptophysin, and SV2A via mutagenesis and optical imaging of pHluorin-tagged proteins in cultured neurons from knock-out mice lacking each protein. 22908222 4 3 gly sites 653:657 arg1 SV2A SV2A sites OGER SV2A Q7L0J3 In this study, we analyzed all glycosylation sites on synaptotagmin 1, synaptophysin, and SV2A via mutagenesis and optical imaging of pHluorin-tagged proteins in cultured neurons from knock-out mice lacking each protein. 19196183 8 44 gly N-glycosylated 1206:1219 arg1 bovine serum albumin bovine serum albumin OGER albumin P02768 Furthermore, unavoidable contaminants such as actin and bovine serum albumin which are not N-glycosylated could be easily depleted by using this glycoproteomic strategy. 9054441 2 49 gly contains 190:197 arg1 Plasminogen 1 AND an N-linked oligosaccharide Plasminogen 1 an N-linked oligosaccharide OGER Plasminogen 1 P00747 Plasminogen 1 contains an N-linked oligosaccharide at Asn-289 and an O-linked oligosaccharide at Thr-345. 26563299 7 30 part_of IL-15 1085:1089 arg1 Asn112 IL-15 Asn71 and Asn112 PUBTATOR AminoAcid IL-15 3600 Asn71 and Asn112 The two potential IL-15 N-glycosylation sites (Asn71 and Asn112) located at the IL-2 receptor interface were unoccupied. 2478297 3 25 gly non-N-glycosylated 792:809 arg1 SY non-N-glycosylated form of SY PUBTATOR non-N-glycosylated form of SY 6855 A non-N-glycosylated form of SY generated by site-directed mutagenesis showed the same behavior and specific distribution in small vesicles. 21763489 5 21 part_of Nt-CCR5 950:956 arg1 Y3, V5, P8-T16 CCR5 Y3, V5, P8-T16 OGER SiteSequence CCR5 P51681 P8-T16 Two-dimensional saturation transfer experiments and measurement of relaxation times highlighted Nt-CCR5 residues Y3, V5, P8-T16, E18, I23 and possibly D2 as the main binding determinant. 8168913 1 81 gly glycoprotein 176:187 arg1 surface glycoprotein A surface glycoprotein A Cterm surface glycoprotein A Pneumocystis carinii surface glycoprotein A (gpA) exhibits host species-specific phenotypic and genotypic variation. 7681247 1 119 gly glycoprotein 305:316 arg1 human alpha 2-HS glycoprotein human alpha 2-HS glycoprotein PUBTATOR alpha 2-HS glycoprotein 197 A complementary DNA (cDNA) for the 59 kD bone sialoprotein, which is supposed to be the rat counterpart of human alpha 2-HS glycoprotein (alpha 2-HSG) and is synthesized by both hepatocytes and osteoblasts, has been cloned from a rat liver cDNA library. 7681247 1 119 gly glycoprotein 305:316 arg1 alpha 2-HSG alpha 2-HSG PUBTATOR alpha 2-HSG 197 A complementary DNA (cDNA) for the 59 kD bone sialoprotein, which is supposed to be the rat counterpart of human alpha 2-HS glycoprotein (alpha 2-HSG) and is synthesized by both hepatocytes and osteoblasts, has been cloned from a rat liver cDNA library. 19249803 10 41 gly glycan 1330:1335 arg1 the WNV E protein protein can glycan OGER protein can P35658 Together these data suggest that loss of the glycan at aa154 on the WNV E protein can severely restrict viral spread in the mosquito vector. 10373415 10 77 gly MUC1 1486:1489 arg1 the conserved tandem repeat domain MUC1 the conserved tandem repeat domain PUBTATOR MUC1 4582 The high incidence of these replacements and their detection also in other cancer cell lines imply that the conserved tandem repeat domain of MUC1 is polymorphic with respect to the peptide sequence. 19796680 0 48 gly desialylation 5:17 arg1 TOLL-like receptor 4 TOLL-like receptor 4 PUBTATOR TOLL-like receptor 4 7099 Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling. 19796680 0 85 gly receptor 84:91 arg1 sialyl alpha-2,3-linked beta-galactosyl residues TOLL-like receptor 4 sialyl alpha-2,3-linked beta-galactosyl residues PUBTATOR TOLL-like receptor 4 7099 Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling. 17653303 5 15 gly glycosylation 803:815 arg1 azurocidin stability azurocidin stability PUBTATOR azurocidin 566 We also demonstrate that N-linked glycosylation contributes to azurocidin stability by protecting it from proteolysis. 8095500 7 26 gly glycosylation 1276:1288 arg1 NPR-A NPR-A PUBTATOR NPR-A 4881 These results suggest a correlation between phosphorylation and complete glycosylation of NPR-A and that both are required for hormone-induced enzymatic activity. 19592704 5 41 gly glycosylation 793:805 arg1 NCEH NCEH PUBTATOR NCEH 57552 All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 19592704 5 53 gly glycosylated 860:871 arg1 NCEH NCEH PUBTATOR NCEH 57552 All of the putative N-linked glycosylation sites (Asn(270), Asn(367), and Asn(389)) of NCEH are glycosylated. 2608056 3 97 gly nonglycosylated 794:808 arg1 Free nonglycosylated LH beta Free nonglycosylated LH beta PUBTATOR LH beta 280839 Free nonglycosylated LH beta, like free wild-type LH beta, was sequestered inside the cell; therefore, the intracellular retention of uncombined LH beta-subunit is not due to a signal located within the N-glycan moiety. 9427547 1 39 gly glycoprotein 129:140 arg1 human trehalase human trehalase PUBTATOR trehalase 11181 A complete cDNA clone encoding human trehalase, a glycoprotein of brush-border membranes, has been isolated from a human kidney library. 16834341 7 2 part_of hFSH 964:967 arg1 Asn52 hFSH Asn52 OGER AminoAcid hFSH Asn52 Also, glycoforms at Asn52 of hFSH are all complex type, whereas in eFSH, both complex and hybrid structures exist at this site. 26812091 3 18 gly glycosylation 395:407 arg1 uhFSH uhFSH Cterm uhFSH Using a glycoproteomic strategy, this study compared the glycosylation of a putative highly purified FSH (uhFSH) obtained from human urine with that of a recombinant human FSH (rhFSH) obtained from Chinese hamster ovary (CHO) cells. 26812091 3 18 gly glycosylation 395:407 arg1 a putative highly purified FSH a putative highly purified FSH OGER FSH Using a glycoproteomic strategy, this study compared the glycosylation of a putative highly purified FSH (uhFSH) obtained from human urine with that of a recombinant human FSH (rhFSH) obtained from Chinese hamster ovary (CHO) cells. 18063813 3 40 gly glycosylated 378:389 arg1 TAFI TAFI PUBTATOR TAFI 1361 TAFI is heavily glycosylated at Asn22, Asn51, Asn63, and Asn86. 12527303 0 45 gly glycoforms 36:45 arg1 human IgG-Fc glycoforms human IgG-Fc glycoforms Cterm IgG Structural analysis of human IgG-Fc glycoforms reveals a correlation between glycosylation and structural integrity. 22159084 10 58 gly Deglycosylation 1249:1263 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 Deglycosylation of SLC26A3 causes a defect in cell surface processing with decreased cell surface expression. 1476702 3 63 gly containing 601:610 arg1 transferrin AND a standard diantennary glycan transferrin a standard diantennary glycan PUBTATOR transferrin 24825 Transferrin bearing the hybrid glycan was degraded in vivo with a half-life of 14 h as compared with 40 h for transferrin containing a standard diantennary glycan. 19931508 0 48 gly glycans 55:61 arg1 inducible costimulator inducible costimulator glycans PUBTATOR inducible costimulator 29851 Definitive evidence that a single N-glycan among three glycans on inducible costimulator is required for proper protein trafficking and ligand binding. 18371226 3 34 gly Glycosylation 474:486 arg1 Fap1 Fap1 OGER Fap1 Q12923 Glycosylation and biogenesis of Fap1 are modulated by a gene cluster downstream of the fap1 locus. 8772226 1 13 gly glycoprotein 180:191 arg1 HRG HRG PUBTATOR HRG 3273 Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immuno-affinity chromatography followed by chromatography with heparin-Sepharose. 23422691 8 60 gly non-glycosylated 1172:1187 arg1 non-glycosylated VEGF non-glycosylated VEGF PUBTATOR VEGF 7422 Furthermore, substitution of asparagine at the VEGF glycosylation site with lysine or glutamic acid increased secretion of non-glycosylated VEGF, a finding not previously reported. 17451431 5 35 gly galectin 1115:1122 arg1 the carbohydrate recognition domain galectin 3 the carbohydrate recognition domain PUBTATOR galectin 3 3958 Furthermore, cell fusion was reduced (specifically) by the disaccharide lactose, a known ligand for the carbohydrate recognition domain of galectin 3, suggesting that the association was functional. 8635486 6 58 gly glycosylation 1206:1218 arg1 HDF HDF OGER HDF P00746 Taken together, our findings suggest an important role of MVA and N-linked glycosylation in PDGF-mediated growth activation of HDF. 8387529 1 19 gly Na 153:154 arg1 a small membrane protein Na,K-ATPase a small membrane protein OGER Na,K-ATPase The gamma subunit of the Na,K-ATPase is a small membrane protein that copurifies with the alpha and beta subunits of the enzyme. 19955571 0 70 gly factor 61:66 arg1 Carbohydrates tissue factor Carbohydrates PUBTATOR tissue factor 2152 Carbohydrates and activity of natural and recombinant tissue factor. 1374238 6 6 gly sialoglycoprotein 826:842 arg1 rLGP85 rLGP85 PUBTATOR rLGP85 117106 It exhibits 86% and 79% sequence similarities in amino acids and nucleic acids to rat lysosomal membrane sialoglycoprotein (rLGP85), respectively. 8817665 8 39 gly fully-glycosylated 1085:1102 arg1 the fully-glycosylated rhIGFBP-3 the fully-glycosylated rhIGFBP-3 OGER rhIGFBP-3 P15473 There appears to be no difference between the mutants and the fully-glycosylated rhIGFBP-3 in their acid-labile subunit (ALS) binding. 11361003 10 48 gly deglycosylated 1842:1855 arg1 deglycosylated TfR deglycosylated TfR PUBTATOR TfR 7037 To assess changes in the secondary structure of the receptor, circular dichroic spectra were recorded from TfR at pH 5.0, from heat pretreated receptor and from deglycosylated TfR. 26742847 3 75 gly N-glycosylated 560:573 arg1 CaVα2δ1 CaVα2δ1 Cterm CaVα2δ1 CaVα2δ1 is potentially the most heavily N-glycosylated subunit in the cardiac L-type CaV1.2 channel complex. 7829269 3 9 gly aglycosylated 777:789 arg1 The resultant aglycosylated chimeric LL2 The resultant aglycosylated chimeric LL2 PUBTATOR LL2 633295 The resultant aglycosylated chimeric LL2 exhibited a similar Raji cell binding affinity to that of the glycosylated form. 21733844 6 53 gly hAQP10 829:834 arg1 All three forms hAQP10 All three forms PUBTATOR hAQP10 89872 All three forms of hAQP10 where found to facilitate the transport of water, glycerol, erythritol, and xylitol, and glycosylation had little effect on functionality. 26197318 5 0 gly NOS1AP 761:766 arg1 Higher O-GlcNAc NOS1AP Higher O-GlcNAc PUBTATOR NOS1AP 192363 Higher O-GlcNAc of NOS1AP was detected during glutamate-induced neuronal apoptosis. 11583740 1 30 gly structures 235:244 arg1 uPCI uPCI structures Cterm uPCI P05154 We investigated the relationship between the procoagulant protease-inhibitory activity and the N-glycan structures in urinary protein C inhibitor (uPCI) by sequential exoglycosidase digestions based on the N-glycan structures elucidated in this report. 11583740 1 30 gly structures 235:244 arg1 urinary protein C inhibitor protein C inhibitor structures OGER protein C inhibitor P05154 We investigated the relationship between the procoagulant protease-inhibitory activity and the N-glycan structures in urinary protein C inhibitor (uPCI) by sequential exoglycosidase digestions based on the N-glycan structures elucidated in this report. 7794919 13 93 gly glycosylated 2395:2406 arg1 The mGRP-R The mGRP-R PUBTATOR mGRP-R 14829 The mGRP-R expressed in these Sf9 cells is incompletely glycosylated and has less complex N-linked oligosaccharide chains, yet it is fully coupled to G proteins and activates phospholipase C, similar to the native receptor, if short infection times are used. 7794919 13 49 gly has 2412:2414 arg1 The mGRP-R AND less complex N-linked oligosaccharide chains The mGRP-R less complex N-linked oligosaccharide chains PUBTATOR mGRP-R 14829 The mGRP-R expressed in these Sf9 cells is incompletely glycosylated and has less complex N-linked oligosaccharide chains, yet it is fully coupled to G proteins and activates phospholipase C, similar to the native receptor, if short infection times are used. 26819318 2 3 gly N-glycosylated 323:336 arg1 human CCR7 human CCR7 PUBTATOR CCR7 1236 Here, we demonstrate that human CCR7 is N-glycosylated on 2 specific residues in the N terminus and the third extracellular loop. 11788899 7 22 part_of WNT8B 910:914 arg1 Gly230 WNT8B Gly230 and Arg284 PUBTATOR AminoAcid WNT8B 7479 Gly230 and Arg284 Gly230 and Arg284 of WNT8B were conserved in WNT8A. 25499264 3 62 gly N-glycan 465:472 arg1 the transmembrane envelope glycoprotein gp41 envelope glycoprotein gp41 N-glycan PUBTATOR envelope glycoprotein gp41 100616444 Recently, also an N-glycan on the transmembrane envelope glycoprotein gp41 was shown to be deleted during CBA resistance development. 25499264 3 112 gly glycoprotein 504:515 arg1 the transmembrane envelope glycoprotein gp41 the transmembrane envelope glycoprotein gp41 PUBTATOR envelope glycoprotein gp41 100616444 Recently, also an N-glycan on the transmembrane envelope glycoprotein gp41 was shown to be deleted during CBA resistance development. 26938549 5 44 gly glycosylation 824:836 arg1 human BST-2 human BST-2 PUBTATOR BST-2 684 These results suggest that N-linked glycosylation of human BST-2 is dispensable for intracellular virion retention and imply that this recently discovered intracellular tethering function may be evolutionarily distinguished from the canonical antiviral function of BST-2 by tethering nascent virions at the cell surface. 14658030 0 82 gly N-glycosylation 18:32 arg1 the murine neural cell adhesion molecule NCAM the murine neural cell adhesion molecule NCAM PUBTATOR NCAM 17967 Identification of N-glycosylation sites of the murine neural cell adhesion molecule NCAM by MALDI-TOF and MALDI-FTICR mass spectrometry. 11465086 5 36 gly glycoprotein 773:784 arg1 immunoreactive NB1 glycoprotein immunoreactive NB1 glycoprotein PUBTATOR NB1 glycoprotein 57126 COS-7 cells transfected with the cDNA expressed immunoreactive NB1 glycoprotein. 14573609 2 6 gly glycosylated 398:409 arg1 glycosylated heparanase glycosylated heparanase PUBTATOR heparanase 10855 The predicted amino acid sequence of heparanase includes six putative N-glycosylation sites; however, the precise biochemical role of glycosylated heparanase remains unknown. 24977290 4 76 gly presence 627:634 arg1 plasma derived ADAMTS13 AND putative O-fucosylation, C-mannosylation and N-linked glycosylation sites plasma derived ADAMTS13 putative O-fucosylation, C-mannosylation and N-linked glycosylation sites PUBTATOR ADAMTS13 11093 OBJECTIVES: Here we investigated the presence of putative O-fucosylation, C-mannosylation and N-linked glycosylation sites on plasma derived ADAMTS13. 9008840 8 76 gly glycoforms 1325:1334 arg1 Gp21 Gp21 Cterm Gp21 These observations indicate that the terminal GlcNAc residue in the minor glycoforms of Gp21 is exclusively located in the alpha (1-->3) branch of the Gp21 glycan. 8335694 3 57 gly glycosylation 453:465 arg1 LIF LIF PUBTATOR LIF 60584 The interaction of LIF with ECM-localized binding sites is not dependent upon either glycosylation of LIF or the presence of extracellular glycosyaminoglycans. 19706343 9 12 gly units 1519:1523 arg1 Asn-143 Asn-143 Asn-143 SpecificSite Asn-143 These data indicate that some APS patients have beta2GPI molecules with a reduced number of negatively charged sialic acid units in the glycan structure at Asn-143. 28958711 9 19 gly N- 1437:1438 arg1 SAs SAs N- OGER SAs Q12999 Collectively, our results identified novel N-glycans enriched in SAs on the rhiBSP and demonstrated that SAs at both N- and O-glycans are important for BSP regulation of osteoblast differentiation and mineralization in vitro. 28958711 9 26 gly O-glycans 1444:1452 arg1 SAs SAs O-glycans OGER SAs Q12999 Collectively, our results identified novel N-glycans enriched in SAs on the rhiBSP and demonstrated that SAs at both N- and O-glycans are important for BSP regulation of osteoblast differentiation and mineralization in vitro. 2164608 0 44 gly glycosylation 2:14 arg1 glycoprotein G glycoprotein G OGER glycoprotein G P07996 O glycosylation of glycoprotein G of human respiratory syncytial virus is specified within the divergent ectodomain. 2164608 0 55 gly glycoprotein 19:30 arg1 glycoprotein G glycoprotein G OGER glycoprotein G P07996 O glycosylation of glycoprotein G of human respiratory syncytial virus is specified within the divergent ectodomain. 24291635 3 18 gly hFSH 441:444 arg1 smaller hFSH smaller OGER hFSH The hFSH(21/18) glycoform preparation was significantly smaller than the hFSH(24) preparation and possessed 60% oligomannose glycans, which is unusual for hFSH. 24351798 5 2 part_of m152 1470:1473 arg1 Asn208 m152 positions Asn61 and Asn208 PUBTATOR AminoAcid m152 5657361 positions Asn61 and Asn208 These data add an important functional detail to recent structural analysis of the m152/RAE1g complex that has revealed N-glycosylations at positions Asn61 and Asn208 of m152 distant from the m152/RAE1g interface. 24351798 5 2 part_of m152 1470:1473 arg1 Asn61 m152 positions Asn61 and Asn208 PUBTATOR AminoAcid m152 5657361 positions Asn61 and Asn208 These data add an important functional detail to recent structural analysis of the m152/RAE1g complex that has revealed N-glycosylations at positions Asn61 and Asn208 of m152 distant from the m152/RAE1g interface. 24351798 5 2 part_of m152 1470:1473 arg1 Asn61 m152 positions Asn61 and Asn208 PUBTATOR AminoAcid m152 5657361 positions Asn61 and Asn208 These data add an important functional detail to recent structural analysis of the m152/RAE1g complex that has revealed N-glycosylations at positions Asn61 and Asn208 of m152 distant from the m152/RAE1g interface. 19261610 8 19 gly N-glycosylation 1454:1468 arg1 the beta1 subunit the beta1 subunit PUBTATOR beta1 subunit 10678 Taken together, the results of the present study reveal for the first time that N-glycosylation of the I-like domain of the beta1 subunit is essential to both the heterodimer formation and biological function of the subunit. 23820512 5 39 gly glycoproteins 861:873 arg1 bovine ribonuclease B bovine ribonuclease B Cterm bovine ribonuclease B The condition to effectively collect complex and heterogeneous N-glycans was established on model glycoproteins, bovine ribonuclease B, bovine fetuin, and human serum IgG. 23820512 5 39 gly glycoproteins 861:873 arg1 human serum IgG human serum IgG Cterm IgG The condition to effectively collect complex and heterogeneous N-glycans was established on model glycoproteins, bovine ribonuclease B, bovine fetuin, and human serum IgG. 9346953 14 26 gly N-deacetylase/N-sulfotransferase 2746:2777 arg1 all previously identified forms N-sulfotransferase all previously identified forms PUBTATOR N-sulfotransferase 53315 The two 3-OST species also exhibit approximately 50% similarity with all previously identified forms of the heparan biosynthetic enzyme N-deacetylase/N-sulfotransferase, which suggests that heparan biosynthetic enzymes share a common sulfotransferase domain. 24213971 0 41 gly glycosylation 89:101 arg1 GABAA receptor β2 subunits GABAA receptor β2 subunits PUBTATOR 2 subunits 15130 Co-expression of γ2 subunits hinders processing of N-linked glycans attached to the N104 glycosylation sites of GABAA receptor β2 subunits. 24213971 0 109 gly attached 68:75 arg1 GABAA receptor β2 subunits AND N-linked glycans GABAA receptor β2 subunits N-linked glycans PUBTATOR 2 subunits 15130 Co-expression of γ2 subunits hinders processing of N-linked glycans attached to the N104 glycosylation sites of GABAA receptor β2 subunits. 12039072 1 61 gly glycoprotein 209:220 arg1 Human sex hormone-binding globulin Human sex hormone-binding globulin PUBTATOR Human sex hormone-binding globulin 6462 Human sex hormone-binding globulin (SHBG) is a homodimeric plasma glycoprotein, and each SHBG monomer may have an O-linked oligosaccharide at Thr(7) and up to two N-linked oligosaccharides at Asn(351) and Asn(367). 1996093 0 72 gly glycoprotein 50:61 arg1 gp34 gp34 PUBTATOR gp34 7292 Molecular cloning and characterization of a novel glycoprotein, gp34, that is specifically induced by the human T-cell leukemia virus type I transactivator p40tax. 7529232 3 11 gly glycosylation 396:408 arg1 the cell-cell recognition molecule CD2 the cell-cell recognition molecule CD2 PUBTATOR CD2 914 In this study a systematic analysis of the effect of glycosylation on the ligand-binding properties of the cell-cell recognition molecule CD2, which consists of two IgSF domains, was undertaken. 26797772 5 8 gly TNSALP 847:852 arg1 individual single N-glycan deletion mutants TNSALP individual single N-glycan deletion mutants PUBTATOR TNSALP 249 Using site-directed mutagenesis, we demonstrated that TNSALP has five N-glycans in transfected COS-1 cells and that individual single N-glycan deletion mutants of TNSALP retain the dimeric structure required for ALP activity, excluding the possibility that any single N-glycan plays a vital role in the structure and function of TNSALP. 26797772 5 44 gly has 745:747 arg1 TNSALP AND five N-glycans TNSALP five N-glycans PUBTATOR TNSALP 249 Using site-directed mutagenesis, we demonstrated that TNSALP has five N-glycans in transfected COS-1 cells and that individual single N-glycan deletion mutants of TNSALP retain the dimeric structure required for ALP activity, excluding the possibility that any single N-glycan plays a vital role in the structure and function of TNSALP. 8483933 3 67 part_of CD4 796:798 arg1 the solvent-accessible Phe-43 CD4 the solvent-accessible Phe-43 PUBTATOR SpecificSite CD4 920 Phe-43 The primary mechanism of recognition and binding is the insertion of the solvent-accessible Phe-43 of CD4 into a gp120 solvent-accessible acceptor pit formed by Trp-427, Tyr-435, and the high-mannose oligosaccharide N-linked to Asn-230. 28668641 0 25 gly Glycosylation 0:12 arg1 SERPINA12 SERPINA12 PUBTATOR SERPINA12 145264 Glycosylation of human vaspin (SERPINA12) and its impact on serpin activity, heparin binding and thermal stability. 28668641 0 25 gly Glycosylation 0:12 arg1 human vaspin human vaspin PUBTATOR vaspin 145264 Glycosylation of human vaspin (SERPINA12) and its impact on serpin activity, heparin binding and thermal stability. 7904345 2 4 gly presence 199:206 arg1 rat sCD4 AND identical oligosaccharides rat sCD4 identical oligosaccharides PUBTATOR sCD4 499358 The presence of identical oligosaccharides at the conserved glycosylation site in domain 3 of rat and human sCD4 and the greater abundance of oligomannose and hybrid type glycans at the non-conserved glycosylation site of rat sCD4 clearly indicate that the protein structure influences oligosaccharide processing. 7904345 2 4 gly presence 199:206 arg2 rat sCD4 AND hybrid type glycans rat sCD4 the conserved glycosylation site hybrid type glycans PUBTATOR sCD4 499358 site The presence of identical oligosaccharides at the conserved glycosylation site in domain 3 of rat and human sCD4 and the greater abundance of oligomannose and hybrid type glycans at the non-conserved glycosylation site of rat sCD4 clearly indicate that the protein structure influences oligosaccharide processing. 7904345 2 68 gly glycosylation 395:407 arg1 rat sCD4 rat sCD4 PUBTATOR sCD4 499358 The presence of identical oligosaccharides at the conserved glycosylation site in domain 3 of rat and human sCD4 and the greater abundance of oligomannose and hybrid type glycans at the non-conserved glycosylation site of rat sCD4 clearly indicate that the protein structure influences oligosaccharide processing. 8601595 2 56 gly glycosylation 234:246 arg1 CD44-hyaluronate interaction CD44-hyaluronate interaction PUBTATOR CD44 960 In the present work we examine the role of N-linked glycosylation and Ser-Gly motifs in regulating CD44-hyaluronate interaction. 28474680 4 8 gly YAP 582:584 arg1 An O-GlcNAc site YAP An O-GlcNAc site PUBTATOR YAP 10413 An O-GlcNAc site of YAP was identified at Thr241, and mutating this site decreased the O-GlcNAcylation, stability, and pro-tumorigenic capacities of YAP, while increasing YAP phosphorylation. 28474680 4 44 gly YAP 711:713 arg1 O-GlcNAcylation YAP O-GlcNAcylation PUBTATOR YAP 10413 An O-GlcNAc site of YAP was identified at Thr241, and mutating this site decreased the O-GlcNAcylation, stability, and pro-tumorigenic capacities of YAP, while increasing YAP phosphorylation. 26202417 2 60 gly HA 279:280 arg1 The oligosaccharides HA The oligosaccharides Cterm HA The oligosaccharides of HA can contribute to HA's antigenic characteristics. 2125005 3 31 gly glycosylated 534:545 arg1 glycosylated FSH glycosylated FSH OGER FSH In the same membranes, the FSH (follitropin) receptor reacted well with both glycosylated FSH and DG-oFSH. 7657720 0 36 gly glycosylation 8:20 arg1 human procathepsin D. Human procathepsin D human procathepsin D. Human procathepsin D Cterm human procathepsin D. Human procathepsin D Role of glycosylation in the expression of human procathepsin D. Human procathepsin D carries two N-linked glycosylation sites at asparagine residues 70 and 199, widely separated on the surface of the folded protein. 8349598 5 85 gly acceptors 1172:1180 arg1 transferrin transferrin acceptors PUBTATOR transferrin 7018 We have examined a number of tissues and cultured cell lines for the transfer of sulfate to the trisaccharide acceptor GGnM and transfer of GalNAc to oligosaccharide acceptors on protein which do, human chorionic gonadotropin (hCG), and do not, transferrin (Trf), contain the PXR/K motif. 15814824 8 78 gly MUC1 1615:1618 arg1 the repeat domain MUC1 the repeat domain PUBTATOR MUC1 4582 We were able to show that the natural immunoglobulin G (IgG) responses to the repeat domain of MUC1 in sera from nonmalignant control subjects are preferentially directed to variant repeat clusters. 11181557 1 2 gly glycosylation 267:279 arg1 the envelope protein gp120 the envelope protein gp120 PUBTATOR gp120 155971 We describe mutants of human immunodeficiency virus type-1 (HIV-1) strain NL4-3, which are lacking the thirteenth, fifteenth, or seventeenth sites for N-linked glycosylation (g13, g15, g17) of the envelope protein gp120. 26858738 7 28 gly O-glycans 1438:1446 arg1 plant-produced IgA1 IgA1 O-glycans PUBTATOR IgA1 3493 By co-expression of enzymes required for initiation and elongation of human O-glycosylation it was possible to generate disialylated mucin-type core 1 O-glycans on plant-produced IgA1. 8925908 1 16 gly glycosylation 104:116 arg1 rat nerve growth factor receptor rat nerve growth factor receptor PUBTATOR nerve growth factor receptor 24596 Here we studied the glycosylation of a mammalian protein, the ectodomain of rat nerve growth factor receptor (NGFRe), in Saccharomyces cerevisiae. 23554678 6 15 part_of Leu517 779:784 arg1 EMR2 EMR2 Leu517 OGER AminoAcid EMR2 Q9UHX3 Leu517 and Ser518 Eight amino acids are involved in N-glycosylation sites and two cleavage sites are Leu517 and Ser518 in EMR2. 23554678 6 26 part_of Ser518 790:795 arg1 EMR2 EMR2 Ser518 OGER AminoAcid EMR2 Q9UHX3 Leu517 and Ser518 Eight amino acids are involved in N-glycosylation sites and two cleavage sites are Leu517 and Ser518 in EMR2. 16469696 3 30 gly DC-SIGN 512:518 arg1 the carbohydrate recognition domain DC-SIGN the carbohydrate recognition domain OGER DC-SIGN Q9NNX6 The structure of DENV in complex with the carbohydrate recognition domain (CRD) of DC-SIGN was determined by cryo-electron microscopy at 25 A resolution. 18082642 8 12 gly glycosylated 1128:1139 arg1 Bovine placental lactogen Bovine placental lactogen OGER Bovine placental lactogen P0DML2 Bovine placental lactogen is a glycosylated hormone, exhibiting somatotropin- and prolactin-like activities. 9059515 2 10 gly glycosylated 271:282 arg1 Human LCAT Human LCAT PUBTATOR Human LCAT 3931 Human LCAT is a glycosylated protein, containing 416 amino acids and a proline-rich region at the C-terminus. 1371804 0 46 gly deglycosylation 11:25 arg1 human thyroperoxidase human thyroperoxidase PUBTATOR thyroperoxidase 7173 Effects of deglycosylation of human thyroperoxidase on its enzymatic activity and immunoreactivity. 3248772 3 26 gly nonglycosylated 404:418 arg1 The nonglycosylated Cu-Zn-superoxide dismutase The nonglycosylated Cu-Zn-superoxide dismutase PUBTATOR Cu-Zn-superoxide dismutase 6647 The nonglycosylated Cu-Zn-superoxide dismutase, which was washed through the boronate column, was glycosylated in vitro upon exposure to radioactive or nonradioactive D-glucose. 3248772 3 42 gly glycosylated 498:509 arg1 The nonglycosylated Cu-Zn-superoxide dismutase The nonglycosylated Cu-Zn-superoxide dismutase PUBTATOR Cu-Zn-superoxide dismutase 6647 The nonglycosylated Cu-Zn-superoxide dismutase, which was washed through the boronate column, was glycosylated in vitro upon exposure to radioactive or nonradioactive D-glucose. 16372382 2 57 gly hormone 410:416 arg1 the N-linked oligosaccharides thyroid-stimulating hormone the N-linked oligosaccharides Cterm thyroid-stimulating hormone In this paper we report the first detailed structural characterization of the N-linked oligosaccharides of recombinant human thyroid-stimulating hormone (rhTSH). 11544325 4 9 gly glycosylation 582:594 arg1 MD-2 MD-2 PUBTATOR MD-2 23643 Mutation of either one of two potential glycosylation sites (Asn(26) and Asn(114)) of MD-2 resulted in the disappearance of the slowest mobility form, and only the fastest form was detected in hMD-2 carrying mutations at both Asn(26) and Asn(114). 7827124 4 1 gly glycosylation 411:423 arg1 LCAT LCAT OGER LCAT P04180 Previous studies of recombinant LCAT have characterized the function of the four N-linked glycosylation sites of LCAT with respect to reconstituted HDL analogue substrates. 12097564 7 51 gly core-glycosylated 1056:1072 arg1 core-glycosylated Env precursor core-glycosylated Env precursor PUBTATOR Env precursor 100616444 We furthermore observed a preference for binding to terminally glycosylated Env over core-glycosylated Env precursor in IPs, suggesting that the epitope is at least partially conformational and dependent on glycosylation. 27314333 11 55 gly N-glycosylation 1736:1750 arg1 Rspo1 Rspo1 PUBTATOR Rspo1 284654 Our findings provide evidence for the critical role of N-glycosylation in the biogenesis of Rspo1. 10845701 0 60 gly glycosylation 11:23 arg1 human bile-salt-stimulated lipase human bile-salt-stimulated lipase PUBTATOR bile-salt-stimulated lipase 1056 Changes in glycosylation of human bile-salt-stimulated lipase during lactation. 2828034 1 14 gly glycoprotein 105:116 arg1 Blast-1 Blast-1 PUBTATOR Blast-1 962 Blast-1 is an early activation-associated glycoprotein expressed on the surface of human lymphocytes. 1737783 6 4 gly unglycosylated 867:880 arg1 unglycosylated CD4 unglycosylated CD4 PUBTATOR CD4 920 Finally, we showed that unglycosylated CD4 produced in HeLa cells was incorrectly folded and retained intracellularly, probably in the endoplasmic reticulum. 26003492 2 66 gly glycoprotein 402:413 arg1 The Her1-ECD The Her1-ECD PUBTATOR Her1-ECD 1956 The Her1-ECD is a glycoprotein with a molecular weight of 105 kDa and has 11 potential sites for N-glycosylation. 1807356 8 34 gly glycosylated 1085:1096 arg1 uninhibited glycosylated renin uninhibited glycosylated renin PUBTATOR renin 5972 In addition, the structure of uninhibited glycosylated renin has been determined at 2.8 A resolution from a cubic crystal form with two renin molecules in the asymmetric unit. 10845701 2 72 gly glycosylated 222:233 arg1 BSSL BSSL PUBTATOR BSSL 1056 BSSL is highly glycosylated and includes one site for N-glycosylation and several sites for O-glycosylation. 17675185 0 96 gly N-glycosylation 12:26 arg1 hepatitis C virus envelope protein E1 hepatitis C virus envelope protein E1 Cterm E1 Deletion of N-glycosylation sites of hepatitis C virus envelope protein E1 enhances specific cellular and humoral immune responses. 1316474 1 14 gly glycoproteins 221:233 arg1 gpI gpI PUBTATOR gpI 2821 The varicella-zoster virus (VZV) genome contains 70 reading frames (ORF), 5 of which encode the glycoproteins gpI, gpII, gpIII, gpIV, and gpV. 1316474 1 14 gly glycoproteins 221:233 arg1 gpV gpV OGER gpV P40197 The varicella-zoster virus (VZV) genome contains 70 reading frames (ORF), 5 of which encode the glycoproteins gpI, gpII, gpIII, gpIV, and gpV. 1316474 1 14 gly glycoproteins 221:233 arg1 gpIV gpIII, gpIV PUBTATOR gpIII, gpIV 51206 The varicella-zoster virus (VZV) genome contains 70 reading frames (ORF), 5 of which encode the glycoproteins gpI, gpII, gpIII, gpIV, and gpV. 29030255 9 113 gly glycosylation 1114:1126 arg1 pufferfish CA VI pufferfish CA VI PUBTATOR CA VI 765 Three potential N-linked glycosylation sites and two cysteine residues (Cys-28 and Cys-209) that are likely to form one disulfide bond were present in pufferfish CA VI. 26869352 8 88 gly released 1379:1386 arg1 haptoglobin AND N-glycan alditols haptoglobin N-glycan alditols PUBTATOR haptoglobin 3240 We next analyzed N-glycan alditols released from haptoglobin using graphitized carbon column LC-ESI MS to identify the linkage of fucosylation. 7559574 0 23 gly oligosaccharides 31:46 arg1 phosphacan phosphacan oligosaccharides PUBTATOR phosphacan 5803 Complex-type asparagine-linked oligosaccharides on phosphacan and protein-tyrosine phosphatase-zeta/beta mediate their binding to neural cell adhesion molecules and tenascin. 18416605 5 46 gly glycosylation 1141:1153 arg1 host PrP host PrP OGER PrP P32119 We have further dissected the requirement of each glycosylation site and have shown that different TSE strains have dramatically different requirements for each of the glycosylation sites of host PrP, and moreover, we have shown that the host PrP has a major role in determining the glycosylation state of de novo generated PrP(Sc). 18416605 5 58 gly glycosylation 1256:1268 arg1 Sc Sc Cterm Sc P32119 We have further dissected the requirement of each glycosylation site and have shown that different TSE strains have dramatically different requirements for each of the glycosylation sites of host PrP, and moreover, we have shown that the host PrP has a major role in determining the glycosylation state of de novo generated PrP(Sc). 18416605 5 58 gly glycosylation 1256:1268 arg1 de novo generated PrP de novo generated PrP OGER PrP P32119 We have further dissected the requirement of each glycosylation site and have shown that different TSE strains have dramatically different requirements for each of the glycosylation sites of host PrP, and moreover, we have shown that the host PrP has a major role in determining the glycosylation state of de novo generated PrP(Sc). 19760176 5 37 gly glycoprotein 887:898 arg1 Gn Gn Cterm Gn The N terminal glycoprotein (Gn) encoded by the M segment contained the 18 conserved Cysteines present in Bunyamwera and California serogroups, the two glycosylation sites, and residues considered potential proteolytic cleavage sites conserved in other Bunyaviridae. 17195076 3 29 gly MUC1 434:437 arg1 their sialylated forms MUC1 their sialylated forms OGER MUC1 P15941 During malignant transformation, certain glyco-epitopes of MUC1, such as Tn-antigen, TF-antigen and their sialylated forms become exposed. 28104755 8 50 gly deglycosylation 1210:1224 arg1 purified recombinant SMPDL3A purified recombinant SMPDL3A PUBTATOR SMPDL3A 10924 Enzymatic deglycosylation of purified recombinant SMPDL3A also resulted in significant loss of phosphodiesterase activity. 9720213 2 1 gly glycosylated 271:282 arg1 LIF LIF PUBTATOR LIF 60584 LIF has been reported to be heavily glycosylated. 9054441 0 0 part_of plasminogen 75:85 arg1 Ser-248 plasminogen 2 Ser-248 OGER SpecificSite plasminogen 2 P00747 Ser-248 Evidence for a novel O-linked sialylated trisaccharide on Ser-248 of human plasminogen 2. 29303997 2 21 gly glycosylation 384:396 arg1 tetherin function tetherin function PUBTATOR tetherin 684 While the role of specific tetherin domains in antiviral activity is clearly established, the role of glycosylation in tetherin function is not clear. 2514791 5 7 gly glycoprotein 1623:1634 arg1 the t-PA glycoprotein the t-PA glycoprotein PUBTATOR t-PA glycoprotein 5327 These results indicate that the t-PA glycoprotein is secreted by each cell line as a set of glycoforms, each glycoform being unique with respect to the nature and disposition of oligosaccharides on a common polypeptide. 10828016 4 31 gly N-glycosylation 460:474 arg1 GMRalpha GMRalpha PUBTATOR GMRalpha 1438 Previously, it was found that N-glycosylation of GMRalpha is essential for ligand binding. 2917524 1 29 gly glycosylated 110:121 arg1 glycosylated PRL PRL PUBTATOR PRL 5617 Two forms of glycosylated PRL (G-PRL) which differed in their binding properties to Concanavalin-A (Con-A) were isolated from human pituitary glands. 11331002 0 48 part_of protein 72:78 arg1 the asparagine117 residue receptor activity-modifying protein 1 the asparagine117 residue PUBTATOR AminoAcid receptor activity-modifying protein 1 10267 asparagine117 residue Mutations of the asparagine117 residue of a receptor activity-modifying protein 1-dependent human calcitonin gene-related peptide receptor result in selective loss of function. 27565712 1 19 gly N-Glycosylation 104:118 arg1 integrin α5β1 integrin α5β1 PUBTATOR 1 3779 N-Glycosylation of integrin α5β1 plays important roles in cell biologic functions; however, the mechanisms that underlie those roles remain poorly understood. 25374123 2 78 gly glycoprotein 333:344 arg1 Vitronectin Vitronectin PUBTATOR Vitronectin 7448 Vitronectin is well known to be a multifunctional glycoprotein in the blood and the extracellular matrix, which is related to hepatocellular carcinoma (HCC). 16368742 5 83 part_of TRPV4 974:978 arg1 Residue N651 TRPV4 Residue N651 PUBTATOR SpecificSite TRPV4 59341 N651 Residue N651 of TRPV4 is immediately adjacent to the pore-forming loop. 18691028 1 92 gly glycoprotein 219:230 arg1 Env Env PUBTATOR Env 155971 A good understanding about the structure and function of the envelope glycoprotein (Env) from primary human immunodeficiency virus-1 (HIV-1) isolates is important in facilitating the development of effective neutralizing antibody responses as a component of an effective HIV-1 vaccine. 18691028 1 92 gly glycoprotein 219:230 arg1 the envelope glycoprotein the envelope glycoprotein PUBTATOR envelope glycoprotein 155971 A good understanding about the structure and function of the envelope glycoprotein (Env) from primary human immunodeficiency virus-1 (HIV-1) isolates is important in facilitating the development of effective neutralizing antibody responses as a component of an effective HIV-1 vaccine. 25701785 8 10 gly glycosylation 1040:1052 arg1 CD83 CD83 PUBTATOR CD83 9308 Mutation of this TELV-motif not only disrupted binding to GRASP55, but also altered the glycosylation pattern of CD83 and reduced its membrane expression. 7599134 0 35 part_of antithrombin 25:36 arg1 antithrombin III asparagine-135 antithrombin III antithrombin III asparagine-135 PUBTATOR SpecificSite antithrombin III 462 asparagine-135 Partial glycosylation of antithrombin III asparagine-135 is caused by the serine in the third position of its N-glycosylation consensus sequence and is responsible for production of the beta-antithrombin III isoform with enhanced heparin affinity. 18725942 2 38 gly glycosylated 308:319 arg1 PTS PTS OGER PTS Q03393 These proteins are characterized by poorly conserved repeated sequences that are rich in prolines and potentially glycosylated threonines and serines (PTS). 12135558 5 58 gly glycosylated 622:633 arg1 The rMCP7 The rMCP7 PUBTATOR rMCP7 54271 The rMCP7 was glycosylated and treatment with N-glycosidase F resulted in a protein of the predicted molecular mass of 30 kDa. 8104165 1 18 gly P-glycoprotein 185:198 arg1 the human MDR1 P-glycoprotein the human MDR1 P-glycoprotein PUBTATOR MDR1 P-glycoprotein 5243 Monoclonal antibodies (MAbs) recognizing external epitopes of the human MDR1 P-glycoprotein have been used both for the detection of multidrug-resistant cells and as specific inhibitors of P-glycoprotein-mediated multidrug resistance. 2116966 0 43 gly glycosylation 17:29 arg1 renin renin PUBTATOR renin 5972 The influence of glycosylation on the fate of renin expressed in Xenopus oocytes. 3651384 0 61 gly glycoprotein 47:58 arg1 sulfated glycoprotein 2 sulfated glycoprotein 2 PUBTATOR sulfated glycoprotein 2 24854 Biosynthesis and molecular cloning of sulfated glycoprotein 2 secreted by rat Sertoli cells. 7578225 8 23 part_of albumin 1167:1173 arg1 normal albumin Asp-63 albumin normal albumin Asp-63 OGER SpecificSite albumin P02768 Asp-63 In normal albumin Asp-63 is exposed and is adjacent to the first disulfide bond, Cys-62-->Cys-53. 24058541 0 28 gly N-glycosylation 12:26 arg1 lysosomal protein CLN5 lysosomal protein CLN5 OGER protein CLN5 O75503 The role of N-glycosylation in folding, trafficking, and functionality of lysosomal protein CLN5. 22326797 8 51 gly glycosylation 1154:1166 arg1 P. pastoris expressed soluble porcine CTLA-4 P. pastoris expressed soluble porcine CTLA-4 PUBTATOR CTLA-4 397286 Glycosylation analysis using PNGase F demonstrated the N-linked glycosylation on P. pastoris expressed soluble porcine CTLA-4. 1883960 0 91 gly glycosylation 41:53 arg1 human interleukin-6 human interleukin-6 PUBTATOR interleukin-6 3569 Marked cell-type-specific differences in glycosylation of human interleukin-6. 26354950 4 54 gly glycosylated 810:821 arg1 FST-ΔHBS-Fc FST-ΔHBS-Fc OGER FST P47931 The data presented indicate that FST-ΔHBS-Fc is heterogeneously glycosylated at the three putative sites in FST when recombinantly expressed in stably transfected Chinese hamster ovary cells. 15628971 7 83 gly glycosylation 1461:1473 arg1 recombinant C4ST recombinant C4ST PUBTATOR C4ST 314694 In addition, the N-linked oligosaccharide at the C-terminal region appears to affect the glycosylation pattern of recombinant C4ST; a broad protein band of the wildtype protein resulting from microheterogeneity of N-linked oligosaccharides disappeared and four discrete protein bands with different numbers of N-linked oligosaccharides appeared when the N-linked oligosaccharide at the C-terminal region was deleted. 8364230 2 36 gly glycosylation 610:622 arg1 PK1de1FE1X PK1de1FE1X Cterm PK1de1FE1X 25692 Furthermore, the effect of the novel plasminogen activator (SUN9216), a plasminogen-plasminogen activator chimera, comprising the fibrin kringle 1 domain of a plasminogen, and the two kringles, and the serine protease domains of wild-type tissue plasminogen activator (t-PA), including a modification of the mannose glycosylation site on the kringle 1 of t-PA (PK1de1FE1X), was studied in this model. 8364230 2 36 gly glycosylation 610:622 arg1 t-PA t-PA PUBTATOR t-PA 25692 Furthermore, the effect of the novel plasminogen activator (SUN9216), a plasminogen-plasminogen activator chimera, comprising the fibrin kringle 1 domain of a plasminogen, and the two kringles, and the serine protease domains of wild-type tissue plasminogen activator (t-PA), including a modification of the mannose glycosylation site on the kringle 1 of t-PA (PK1de1FE1X), was studied in this model. 8364230 2 62 gly t-PA 649:652 arg1 the mannose glycosylation site t-PA the mannose glycosylation site PUBTATOR t-PA 25692 Furthermore, the effect of the novel plasminogen activator (SUN9216), a plasminogen-plasminogen activator chimera, comprising the fibrin kringle 1 domain of a plasminogen, and the two kringles, and the serine protease domains of wild-type tissue plasminogen activator (t-PA), including a modification of the mannose glycosylation site on the kringle 1 of t-PA (PK1de1FE1X), was studied in this model. 21385452 0 64 gly glycosylated 29:40 arg1 aberrantly glycosylated MUC1 aberrantly glycosylated MUC1 PUBTATOR MUC1 4582 Autoantibodies to aberrantly glycosylated MUC1 in early stage breast cancer are associated with a better prognosis. 1284982 1 15 gly alpha-sialoglycoprotein 295:317 arg1 alpha-sialoglycoprotein sialoglycoprotein (alpha OGER sialoglycoprotein (alpha P02724 The human erythrocyte membrane contains four sialoglycoproteins, denoted alpha, beta, gamma and delta (also known as glycophorins A, C, D and B respectively), of which alpha-sialoglycoprotein (alpha-SGP) is the most predominant species. 28467637 0 37 gly N-glycosylation 0:14 arg1 the β2 adrenergic receptor the β2 adrenergic receptor PUBTATOR 2 adrenergic receptor 154 N-glycosylation of the β2 adrenergic receptor regulates receptor function by modulating dimerization. 16224972 1 53 gly erythropoietins 359:373 arg1 the O- and N-glycan structures erythropoietins the O- and N-glycan structures Cterm erythropoietins The structural characterization of the O- and N-glycan structures of three different commercially available recombinant human erythropoietins (rhEPOs) is represented by means of a microscale sample purification using ZipTip technology and MALDI-TOF and MALDI low-energy CID MS. Glycopeptides were released from rhEPO samples by a differential endoproteolytic digestion to obtain site-specific glycosylation patterns. 26701645 4 10 gly N-glycosylated 663:676 arg1 tyrosinase tyrosinase PUBTATOR tyrosinase 7299 By LC-MS/MS analysis of human tyrosinase expressed in a melanoma cell, we show that all seven sites of tyrosinase are at least partially N-glycosylated. 26701645 4 71 gly tyrosinase 629:638 arg1 all seven sites tyrosinase all seven sites PUBTATOR tyrosinase 7299 By LC-MS/MS analysis of human tyrosinase expressed in a melanoma cell, we show that all seven sites of tyrosinase are at least partially N-glycosylated. 8548075 5 24 gly gp120 1057:1061 arg1 oligosaccharides gp120 oligosaccharides OGER gp120 Q14624 This mycoplasma is able to recognize terminal NeuAc alpha 2-3 Gal in the composition of oligosaccharides of gp120, which permits it to adhere HIV virions on itself and then to transport them directly to the cells expressing receptor CD4 and having oligosaccharides of the same terminal structure. 20207824 2 33 part_of Asn13 263:267 arg1 human somatostatin receptor subtype-5 somatostatin receptor subtype-5 Asn13 PUBTATOR AminoAcid somatostatin receptor subtype-5 6755 Asn13 and Asn26 position Focusing on Asn13 and Asn26 positioned on N-linked glycosylation motifs in the amino-terminal domain of human somatostatin receptor subtype-5 (hSSTR5), we performed site-directed mutagenesis and evaluated the mutants by using yeast cells as the host strain. 29888865 9 70 gly N-glycosylation 1318:1332 arg1 human plasma-derived (pd)FVIII human plasma-derived (pd)FVIII PUBTATOR FVIII 2157 Seeking to better understand the glycosylation mechanisms underlying FVIII biology, we studied the N-glycosylation of human plasma-derived (pd)FVIII and six rFVIII products expressed in CHO, BHK or HEK cell lines. 20729838 3 44 gly glycosylation 366:378 arg1 K18 K18 OGER K18 P05783 K18 undergoes dynamic O-linked N-acetylglucosamine glycosylation at Ser 30, 31 and 49. 10580126 11 14 gly alpha1-6-fucosylated 1483:1502 arg1 AFP AFP PUBTATOR AFP 174 Serum alpha1-6-fucosylated alpha-fetoprotein (AFP) has been employed for an early diagnosis of patients with hepatoma. 10580126 11 14 gly alpha1-6-fucosylated 1483:1502 arg1 Serum alpha1-6-fucosylated alpha-fetoprotein Serum alpha1-6-fucosylated alpha-fetoprotein PUBTATOR alpha-fetoprotein 174 Serum alpha1-6-fucosylated alpha-fetoprotein (AFP) has been employed for an early diagnosis of patients with hepatoma. 2608056 7 86 gly glycosylation 1812:1824 arg1 the LH beta-subunit the LH beta-subunit PUBTATOR LH beta 280839 Both proteins displayed similar potency (ED50 = 32 vs. 41 ng/ml, respectively) and maximal stimulation of progesterone release Pmax = 2.7 vs 2.5 micrograms/ml), indicating that N-linked glycosylation of the LH beta-subunit does not play a significant role in LH signal transduction. 2164668 0 36 gly factor 46:51 arg1 Mammalian cell transient expression tissue factor Mammalian cell transient expression PUBTATOR tissue factor 2152 Mammalian cell transient expression of tissue factor for the production of antigen. 8486697 0 56 gly glycoprotein 23:34 arg1 RNA polymerase II RNA polymerase II OGER RNA polymerase II RNA polymerase II is a glycoprotein. 27743359 4 35 gly glycoproteins 767:779 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 27743359 4 35 gly glycoproteins 767:779 arg1 IgG IgG Cterm IgG We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 27743359 4 35 gly glycoproteins 767:779 arg1 transferrin transferrin PUBTATOR transferrin 7018 We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 27743359 4 35 gly glycoproteins 767:779 arg1 IgA IgA OGER IgA P11912 We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 27743359 4 35 gly glycoproteins 767:779 arg1 alpha-2-macroglobulin alpha-2-macroglobulin PUBTATOR alpha-2-macroglobulin 2 We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 27743359 4 35 gly glycoproteins 767:779 arg1 IgM IgM OGER IgM P01871 We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 27743359 4 35 gly glycoproteins 767:779 arg1 complement C3 complement C3 PUBTATOR complement C3 718 We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3. 2006911 1 32 gly attached 312:319 arg2 purified human complement Factor B AND glucose purified human complement Factor B lysine-266 glucose PUBTATOR complement Factor B 629 lysine-266 Evidence is now presented that glucose is covalently attached to lysine-266 of purified human complement Factor B as a result of glycation. 29793953 8 123 gly nonglycosylated 1482:1496 arg1 nonglycosylated NTCP nonglycosylated NTCP PUBTATOR NTCP 6554 In conclusion, nonglycosylated NTCP is expressed by differentiated HepaRG cells and capable of mediating cHBV infection in HepG2 cells, but it cannot explain differential susceptibility of HepaRG and HepG2/NTCP cells to cHBV versus sHBV infection and different HBsAg/HBeAg ratios following cHBV infection. 21575138 4 10 gly glycosylated 471:482 arg1 surface nucleolin surface nucleolin PUBTATOR nucleolin 4691 We found that surface nucleolin is exclusively glycosylated and that N-glycosylation is required for its expression on the cells. 10964928 6 11 gly glycosylated 742:753 arg1 glycosylated pro-LPC glycosylated pro-LPC PUBTATOR AminoAcid LPC 9159 Using a reducible cross-linker, we found that glycosylated pro-LPC is associated with the molecular chaperone BiP. 20668520 8 87 gly fOS 1625:1627 arg1 a small but significant Engase1p-mediated generation fOS a small but significant Engase1p-mediated generation PUBTATOR fOS 2353 In metabolically radiolabeled HepG2 cells evidence was obtained for a small but significant Engase1p-mediated generation of fOS in 1 h chase but not 30 min pulse incubations. 23661698 8 6 part_of EphA2 1196:1200 arg1 Asp-78 EphA2 Asp-78 and Lys-136 PUBTATOR SpecificSite EphA2 1969 Asp-78 and Lys-136 Analysis of Eph/ephrin crystal structures reveals an interaction between the ligand's carbohydrates and two residues of EphA2: Asp-78 and Lys-136. 8494888 7 18 gly glycosylation 1281:1293 arg1 CETP CETP PUBTATOR CETP 1071 To explore this hypothesis further, each of the four potential N-linked glycosylation sites of CETP (at amino acid positions 88, 240, 341, and 396) was eliminated by mutagenesis of asparagine to glutamine. 21471242 5 26 gly glycosylated 1050:1061 arg1 gp160ER gp160ER Cterm gp160ER A major VLP contaminant was found to consist of an early, monomeric form of gp160 that is glycosylated in the endoplasmic reticulum (gp160ER) and then bypasses protein maturation and traffics directly into particles. 21471242 5 26 gly glycosylated 1050:1061 arg1 an early, monomeric form an early, monomeric form PUBTATOR form of gp160 2028 A major VLP contaminant was found to consist of an early, monomeric form of gp160 that is glycosylated in the endoplasmic reticulum (gp160ER) and then bypasses protein maturation and traffics directly into particles. 21598331 0 22 gly P-glycoprotein 91:104 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Liquid chromatography/tandem mass spectrometry based targeted proteomics quantification of P-glycoprotein in various biological samples. 15917430 6 68 gly gp120 1194:1198 arg1 high mannose glycans gp120 high mannose glycans PUBTATOR gp120 3700 Molecular structure comparison for Man9GlcNAc2 recognition by ConA and 2G12 indicates that 2G12 has a more restricted specificity to high mannose glycans of gp120 which correlates with kinetic analysis assessed by surface plasmon resonance (SPR) and ConA inhibits 2G12 binding to gp120 but 2G12 does not inhibit ConA binding to gp120. 1702808 9 69 gly aglycosylated 1201:1213 arg1 aglycosylated IgG1 aglycosylated IgG1 OGER IgG1 P01857 Glycosylated and aglycosylated IgG1, 2, and 4 were bound identically by monoclonal and polyclonal RF. 18214858 1 30 gly fucosylated 213:223 arg1 fucosylated haptoglobin fucosylated haptoglobin PUBTATOR haptoglobin 3240 It was found in our previous studies that the concentration of fucosylated haptoglobin had increased in the sera of patients with pancreatic cancer (PC) compared to those of other types of cancer and normal controls. 20507986 5 35 gly glycosylation 790:802 arg1 purified rabbit skeletal muscle alpha-DG purified rabbit skeletal muscle alpha-DG Cterm alpha-DG Q14118 Here, we explore the glycosylation of purified rabbit skeletal muscle alpha-DG in detail. 8180202 8 9 gly glycosylation 1333:1345 arg1 CBG CBG PUBTATOR CBG 866 As we have previously found, glycosylation at Asn238 is essential for the production of CBG with steroid-binding activity, but when the mutant containing only one oligosaccharide at this position was enzymatically deglycosylated, its steroid-binding activity was unaltered. 18214858 3 37 gly haptoglobin 600:610 arg1 site-specific N-glycan structures haptoglobin site-specific N-glycan structures PUBTATOR haptoglobin 3240 In the present study, site-specific N-glycan structures of haptoglobin in sera obtained from patients with PC or chronic pancreatitis (CP) were analyzed using liquid chromatography-electrospray ionization mass spectrometry. 1904059 9 114 gly observed 1249:1256 arg1 plasma factor VII AND the three glycan structures plasma factor VII the three glycan structures OGER factor VII P08709 Approximately equal amounts of the three glycan structures were observed in plasma factor VII, whereas in recombinant factor VII the glucose and the glucose-(xylose)2 structures predominated. 23765987 7 69 gly glycoforms 899:908 arg1 HPX HPX PUBTATOR HPX 3263 Quantification of the minor glycoforms of HPX and glycoforms of sex hormone binding globulin required enrichment of the protein because these analytes were below the sensitivity of the 4000 quadrupole ion trap hybrid mass spectrometer in the complex serum background. 23765987 7 76 gly glycoforms 921:930 arg1 HPX HPX PUBTATOR HPX 3263 Quantification of the minor glycoforms of HPX and glycoforms of sex hormone binding globulin required enrichment of the protein because these analytes were below the sensitivity of the 4000 quadrupole ion trap hybrid mass spectrometer in the complex serum background. 10734111 4 15 gly factor 740:745 arg1 4-GlcNAc-beta1,3-Fuc-alpha1-O-Ser/Thr factor IX 4-GlcNAc-beta1,3-Fuc-alpha1-O-Ser/Thr OGER factor IX P00740 Through exoglycosidase digestions we determined that the O-linked fucose oligosaccharide is a tetrasaccharide with a structure identical to that found on human clotting factor IX: Sia-alpha2,3-Gal-beta1, 4-GlcNAc-beta1,3-Fuc-alpha1-O-Ser/Thr. 19507852 5 83 gly proteins 1245:1252 arg1 the modified galactose scFv proteins the modified galactose PUBTATOR scFv proteins 652070 These fusion scFv proteins with the modified galactose are then conjugated with a fluorescence probe, Alexa488, that carries an orthogonal reactive group. 10682309 11 76 gly deglycosylated 1766:1779 arg1 the wild-type SAP-B the wild-type SAP-B OGER SAP O60880 SAP-B in the patient's cells was found to be slightly less stable than the protein in normal cells and corresponded in size to the deglycosylated form of the wild-type SAP-B. 26563299 6 2 part_of IL-15 881:885 arg1 IL-15 Asn79 IL-15 IL-15 Asn79 PUBTATOR AminoAcid IL-15 3600 Asn79 IL-15 Asn79 and sIL-15Rα Asn107 carried the same repertoire of biosynthetically-related N-glycans covering mostly α1-6-core-fucosylated and β-GlcNAc-terminating complex-type structures. 26563299 6 93 part_of sIL-15Rα 897:904 arg1 sIL-15Rα Asn107 sIL sIL-15Rα Asn107 OGER AminoAcid sIL Q15468 Asn107 IL-15 Asn79 and sIL-15Rα Asn107 carried the same repertoire of biosynthetically-related N-glycans covering mostly α1-6-core-fucosylated and β-GlcNAc-terminating complex-type structures. 12702494 5 30 gly underglycosylation 699:716 arg1 Oatp1 Oatp1 OGER Oatp1 Q99N01 To assess whether underglycosylation of Oatp1 in yeast suppressed functional activity, Oatp1 was expressed in Xenopus laevis oocytes with and without tunicamycin, a glycosylation inhibitor. 9720213 1 4 gly glycoprotein 154:165 arg1 leukemia inhibitory factor leukemia inhibitory factor PUBTATOR leukemia inhibitory factor 60584 The leukemia inhibitory factor (LIF) is a secretory glycoprotein and a pluripotent growth factor which acts in diverse cell systems. 8961954 9 20 part_of bPTH 1304:1307 arg1 Lys13 PTH Lys13 PUBTATOR AminoAcid PTH 5741 Lys13 In addition, the highly potent benzophenone (pBz2)-containing PTH-derived radioligand [Nle8,18,Lys13(epsilon-pBz2),L-2-Nal23,Tyr34 3-125I)]bPTH(1-34)NH2 can photoaffinity cross-link specifically to the nonglycosylated receptor. 8961954 9 48 part_of PTH-derived 1227:1237 arg1 Lys13 PTH Lys13 PUBTATOR AminoAcid PTH 5741 Lys13 In addition, the highly potent benzophenone (pBz2)-containing PTH-derived radioligand [Nle8,18,Lys13(epsilon-pBz2),L-2-Nal23,Tyr34 3-125I)]bPTH(1-34)NH2 can photoaffinity cross-link specifically to the nonglycosylated receptor. 7706290 2 22 gly non-glycosylated 463:478 arg1 recombinant non-glycosylated interleukin 4 recombinant non-glycosylated interleukin 4 PUBTATOR interleukin 4 16189 Using an inducible recA promoter and the g10-L ribosome-binding site, recombinant non-glycosylated interleukin 4 (IL-4) was expressed as 17% of total cellular protein in Escherichia coli inclusion bodies, as a reduced, inactive 14.5-kDa polypeptide. 7706290 2 22 gly non-glycosylated 463:478 arg1 IL-4 IL-4 PUBTATOR IL-4 16189 Using an inducible recA promoter and the g10-L ribosome-binding site, recombinant non-glycosylated interleukin 4 (IL-4) was expressed as 17% of total cellular protein in Escherichia coli inclusion bodies, as a reduced, inactive 14.5-kDa polypeptide. 28279966 0 14 part_of K1491-R1492 41:51 arg1 Von Willebrand Factor Plasmin Cleaves Von Willebrand Factor K1491-R1492 PUBTATOR SiteSequence Plasmin Cleaves Von Willebrand Factor 5340 K1491-R1492 Plasmin Cleaves Von Willebrand Factor at K1491-R1492 in the A1-A2 Linker Region in a Shear- and Glycan-Dependent Manner In Vitro. 1962503 9 28 gly glycoproteins 1708:1720 arg1 G1 G1 Cterm G1 This recombinant virus expressed two glycoproteins G1 and G2 identical to R22 virus G1 and G2 in molecular weight, cleavage pattern and cellular immunofluorescent patterns. 8757293 6 30 gly PR3 936:938 arg1 The experimentally observed preference PR3 The experimentally observed preference PUBTATOR PR3 5657 The experimentally observed preference of PR3 for small aliphatic residues at the P1 position of a substrate is explained by the Val to Ile substitution at position 190 when compared to the elastase structure. 26272216 7 47 gly mannosylated 1132:1143 arg1 highly mannosylated BCR highly mannosylated BCR OGER BCR P11274 Moreover, we underlined that a subset of IgM(+) FL samples, displaying highly mannosylated BCR, efficiently bound dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), which could in turn trigger delayed but long-lasting BCR aggregation and activation. 18563860 7 25 gly rhEPO 1703:1707 arg1 commercially available pharmaceutical preparations rhEPO commercially available pharmaceutical preparations OGER rhEPO P29676 The technique described herein provides a mean to detect glycopeptides from commercially available pharmaceutical preparations of rhEPO with the sensitivity required to analyze pmol amounts of hEPO, which could ultimately lead to the identification of structural differences between the recombinant and the human forms of the hormone. 8499451 6 61 gly nonglycosylated 1274:1288 arg1 up to 25 mg/L nonglycosylated transferrin up to 25 mg/L nonglycosylated transferrin PUBTATOR transferrin 7018 Mutation of the two asparagine carbohydrate linkage sites to aspartic acid residues led to the expression and secretion of up to 25 mg/L nonglycosylated transferrin. 17544837 5 48 gly glycoprotein 729:740 arg1 The secreted shFas-Fc The secreted shFas-Fc Cterm shFas P25445 The secreted shFas-Fc was shown to be a glycoprotein with heterogeneous glycan chains. 10531415 0 46 gly glycosylation 9:21 arg1 D1 D1 Cterm D1 N-linked glycosylation is required for plasma membrane localization of D5, but not D1, dopamine receptors in transfected mammalian cells. 8955058 8 25 gly glycosylated 1648:1659 arg1 glycosylated gag glycosylated gag PUBTATOR gag 17276 These results indicate that glycosylated gag facilitates virus spread within the spleen and to extra-splenic sites, such as the CNS, and suggest that the protein may function through its interaction with the host. 11159927 5 9 gly sialylated 571:580 arg1 Only IgG1-Pro-5 Only IgG1-Pro-5 PUBTATOR SpecificSite Only IgG1 105243590 Only IgG1-Pro-5 was sialylated with sialic acid present on only a small percentage of the carbohydrate structures. 11549261 2 19 gly MUC2 500:503 arg1 the tandem repeat MUC2 the tandem repeat PUBTATOR MUC2 4583 In this paper, four human pp-GalNAc-Ts (pp-GalNAc-T1, T2, T3, and T4) were tested for their preferential orders of GalNAc incorporation into FITC-PTTTPITTTTK, a portion of the tandem repeat of human MUC2. 10561578 5 62 gly N-glycosylated 984:997 arg1 high-mannose N-glycosylated high-mannose Cterm N-glycosylated The kinetic parameters of the nonglycosylated mutant were found to be essentially equivalent to those of natural enzymes N-glycosylated with either high-mannose or complex-type oligosaccharides. 10561578 5 62 gly N-glycosylated 984:997 arg1 complex-type oligosaccharides N-glycosylated complex-type oligosaccharides Cterm N-glycosylated The kinetic parameters of the nonglycosylated mutant were found to be essentially equivalent to those of natural enzymes N-glycosylated with either high-mannose or complex-type oligosaccharides. 11557028 12 30 gly glycosylated 1824:1835 arg1 hLAT1 hLAT1 PUBTATOR hLAT1 8140 Finally, in in vitro translation, we show that hLAT1 is not a glycosylated protein even though an N-glycosylation site has been predicted in its extracellular loop, consistent with the property of the classical 4F2 light chain. 6386981 2 4 gly glycosylation 505:517 arg1 CHO-rHuIFN-beta IFN-beta PUBTATOR IFN-beta 3456 SDS-PAGE differentiates E. coli-rHuIFN-beta ser (Mr = 17,000) from CHO-rHuIFN-beta and HuIFN-beta (Mr = 23,000), with glycosylation accounting for 26% of the apparent m.w. of the latter two proteins. 21977518 0 37 gly glycosylated 95:106 arg1 beta1 integrin beta1 integrin PUBTATOR beta1 integrin 3688 Effects of altered restraints in beta1 integrin on the force-regulated interaction between the glycosylated I-like domain of beta1 integrin and fibronectin III9-10: a steered molecular dynamic study. 21977518 0 37 gly glycosylated 95:106 arg1 fibronectin III9-10 fibronectin III9-10 PUBTATOR fibronectin III9-10 2335 Effects of altered restraints in beta1 integrin on the force-regulated interaction between the glycosylated I-like domain of beta1 integrin and fibronectin III9-10: a steered molecular dynamic study. 22607976 3 47 gly nonglycosylated 460:474 arg1 transthyretin transthyretin PUBTATOR transthyretin 7276 We report here the cryptic N-glycosylation site as a recognition signal for unfolding of a natively nonglycosylated protein, transthyretin (TTR), involved in familial amyloidosis. 25327667 0 54 gly glycosylation 33:45 arg1 prostate specific antigen prostate specific antigen PUBTATOR prostate specific antigen 354 Glycoproteomics: identifying the glycosylation of prostate specific antigen at normal and high isoelectric points by LC-MS/MS. 29209650 2 21 gly glycosylation 310:322 arg1 KEAP1 KEAP1 PUBTATOR KEAP1 9817 We discovered that NRF2 signaling is controlled by the site-specific glycosylation of KEAP1, revealing a potentially broad link among nutrient sensing, proteostasis and stress resistance in both normal and cancer cells. 28322444 5 16 gly N220-glycosylated 946:962 arg1 N220-glycosylated Kv3.1b. N220-glycosylated Kv3.1b. Cterm Kv3.1b Mass spectrometry analysis revealed a complex type Hex3 HexNAc4 Fuc1 glycan as the major glycan component of the N229-glycosylated Kv3.1b protein, as opposed to a high-mannose type Man8 GlcNAc2 glycan for N220-glycosylated Kv3.1b. Taken together, these results suggest that trafficking-dependent roles of the Kv3.1b potassium channel are dependent on N229 site-specific glycosylation and N-glycan structure, and operate through a mechanism whereby specific N-glycan structures regulate cell surface expression. 12888867 4 22 gly non-glycosylated 575:590 arg1 non-glycosylated PAI-1 non-glycosylated PAI-1 PUBTATOR PAI-1 5054 The IC(50)-values for inactivation of PAI-1 by 4 monoclonal antibodies differed strongly between glycosylated PAI-1 and non-glycosylated PAI-1 expressed in E. coli. 12888867 4 50 gly glycosylated 552:563 arg1 glycosylated PAI-1 glycosylated PAI-1 PUBTATOR PAI-1 5054 The IC(50)-values for inactivation of PAI-1 by 4 monoclonal antibodies differed strongly between glycosylated PAI-1 and non-glycosylated PAI-1 expressed in E. coli. 8130392 9 68 gly oligosaccharides 1429:1444 arg1 rHPC are rHPC oligosaccharides OGER rHPC P52873 The structure of the major neutral oligosaccharide in rHPC was determined to be: [formula: see text] Two representatives of the sialylated oligosaccharides in rHPC are: [formula: see text] and [formula: see text] Thus, many of the Asn-linked oligosaccharides in rHPC were found to terminate in GalNAc beta (1-->4)GlcNAc beta (1-->.) 8130392 9 86 gly oligosaccharides 1532:1547 arg1 rHPC rHPC oligosaccharides OGER rHPC P52873 The structure of the major neutral oligosaccharide in rHPC was determined to be: [formula: see text] Two representatives of the sialylated oligosaccharides in rHPC are: [formula: see text] and [formula: see text] Thus, many of the Asn-linked oligosaccharides in rHPC were found to terminate in GalNAc beta (1-->4)GlcNAc beta (1-->.) 8130392 9 128 gly oligosaccharide 1325:1339 arg1 rHPC rHPC oligosaccharide OGER rHPC P52873 The structure of the major neutral oligosaccharide in rHPC was determined to be: [formula: see text] Two representatives of the sialylated oligosaccharides in rHPC are: [formula: see text] and [formula: see text] Thus, many of the Asn-linked oligosaccharides in rHPC were found to terminate in GalNAc beta (1-->4)GlcNAc beta (1-->.) 15869464 3 61 gly glycosylation 664:676 arg1 native human OPN native human OPN PUBTATOR OPN 6696 Using a combination of Edman degradation and MS analyses, we have characterized the complete phosphorylation and glycosylation pattern of native human OPN. 8737716 0 51 gly Microheterogeneity 0:17 arg1 alpha 1-antitrypsin alpha 1-antitrypsin PUBTATOR alpha 1-antitrypsin 5265 Microheterogeneity of alpha 1-antitrypsin in relation to the concentration of its complex with immunoglobulin A in the sera of patients with rheumatoid arthritis. 18203712 1 18 gly N-glycosylated 102:115 arg1 Cyclooxygenases Cyclooxygenases PUBTATOR Cyclooxygenases (COX-1 4512 Cyclooxygenases (COX-1 and COX-2) are N-glycosylated, endoplasmic reticulum-resident, integral membrane proteins that catalyze the committed step in prostanoid synthesis. 17082223 1 67 gly glycosylation 185:197 arg1 the bile salt export pump [Bsep the bile salt export pump [Bsep OGER bile salt export pump O70127 The aim of this study was to determine the role of N-linked glycosylation in protein stability, intracellular trafficking, and bile acid transport activity of the bile salt export pump [Bsep (ATP-binding cassette B11)]. 27649061 2 102 gly glycoforms 353:362 arg1 IgG IgG Cterm IgG Multiple analytical methods have been designed for the determination of the IgG N-glycan microheterogeneity, including MS methods for the analysis of site specific glycoforms of IgG. 8419363 0 43 gly Glycosylation 0:12 arg1 human corticosteroid-binding globulin human corticosteroid-binding globulin PUBTATOR corticosteroid-binding globulin 866 Glycosylation of human corticosteroid-binding globulin at aspargine 238 is necessary for steroid binding. 24361341 6 20 gly Deglycosylated 961:974 arg1 Deglycosylated NOX1 Deglycosylated NOX1 PUBTATOR Deglycosylated NOX1 114243 Deglycosylated NOX1 migrated at ~53kDa and N-glycosylation was demonstrated in NOX1 derived from both rat and human. 10411623 1 64 gly glycosylated 111:122 arg1 Human gastric lipase Human gastric lipase PUBTATOR Human gastric lipase 8513 Human gastric lipase (HGL) is a highly glycosylated protein, as glycan chains account for about 15% of the molecular mass of the native HGL. 17451431 4 26 gly glycosylated 814:825 arg1 CD98hc CD98hc PUBTATOR CD98hc 6520 Western blotting showed that CD98 and galectin 3, a proposed ligand for the glycosylated extracellular domain of CD98hc, co-immunoprecipitated, and double-label immuno-electron microscopy confirmed that CD98hc associated with galectin 3. 2477227 11 46 gly beta 1707:1710 arg1 the underlying N-linked structures hCG beta the underlying N-linked structures PUBTATOR hCG beta 1082 We propose that the N-linked oligosaccharides on beta-core closely resemble the underlying N-linked structures of hCG beta with the antennary sialic acid, galactose, and N-acetylglucosamine removed. 26977294 9 47 gly fucosylation 1358:1369 arg1 Fab-TNF α interactions Fab-TNF α PUBTATOR Fab-TNF α 7124 While fucosylation does not affect Fab-TNF α interactions, we found that in the absence of fucosylation the Fc-mAb domain and Fcγ RIIIA are closer and new strong interactions are established between G129 of the receptor and S301 of the Chimera 2 Fc mAb; new polar interactions are also established between the Chimera 2 Fc residues Y299, N300, and S301 and the Fcγ RIIIA residues K128, G129, R130, and R155. 17451431 6 23 gly N-glycosylation 1213:1227 arg1 CD98 CD98 PUBTATOR CD98 17254 Taken together, the data suggest that N-glycosylation of CD98 and subsequent interaction with galectin 3 is critical for aspects of placental cell biology, and provides a rationale for the observation that, in the mouse, truncation of the CD98hc extracellular domain leads to early embryonic lethality [Tsumura H, Suzuki N, Saito H, Kawano M, Otake S, Kozuka Y, Komada H, Tsurudome M & Ito Y (2003) Biochem Biophys Res Commun 308, 847-851]. 2380335 8 81 gly glycosylation 1284:1296 arg1 hPRL hPRL PUBTATOR hPRL 5617 The modification of the glycosylation pattern of hPRL significantly altered the RIA values for PRL. 19633189 4 17 gly dystrophin-glycoprotein 823:845 arg1 an intact dystrophin-glycoprotein complex an intact dystrophin-glycoprotein complex OGER dystrophin P11532 These phenotypes of dystroglycan-null muscles are recapitulated by Large(myd) muscles, which have an intact dystrophin-glycoprotein complex and lack only the laminin globular domain-binding motif on alpha-dystroglycan. 9378972 8 4 gly glycosylated 1412:1423 arg1 NK cell Fc gamma RIIIa NK cell Fc gamma RIIIa PUBTATOR Fc gamma RIIIa 2214 NK cell Fc gamma RIIIa is glycosylated with high mannose- and complex-type oligosaccharides, while monocyte Fc gamma RIIIa has no high mannose-type oligosaccharides. 9378972 8 90 gly has 1509:1511 arg1 monocyte Fc gamma RIIIa AND no high mannose-type oligosaccharides monocyte Fc gamma RIIIa no high mannose-type oligosaccharides PUBTATOR Fc gamma RIIIa 2214 NK cell Fc gamma RIIIa is glycosylated with high mannose- and complex-type oligosaccharides, while monocyte Fc gamma RIIIa has no high mannose-type oligosaccharides. 11275255 4 64 gly Glycosylation 644:656 arg1 IgG-Fc IgG-Fc Cterm IgG Glycosylation of IgG-Fc has been shown to be essential for efficient activation of FcgammaR and C1. 23259747 2 63 gly repeats 520:526 arg1 human MUC1 glycoprotein MUC1 glycoprotein repeats PUBTATOR MUC1 glycoprotein 4582 This study provides evidence of site-specific structural alteration induced during multiple sialylation at Ser/Thr residues of the tandem repeats in human MUC1 glycoprotein. 23259747 2 51 gly glycoprotein 542:553 arg1 the tandem repeats MUC1 glycoprotein the tandem repeats PUBTATOR MUC1 glycoprotein 4582 This study provides evidence of site-specific structural alteration induced during multiple sialylation at Ser/Thr residues of the tandem repeats in human MUC1 glycoprotein. 2430793 5 61 gly glycoprotein 1035:1046 arg1 the native PAI glycoprotein the native PAI glycoprotein PUBTATOR PAI glycoprotein 18787 wt identical to that of the native PAI glycoprotein (Mr 52,000), whereas in E. coli an unglycosylated, active product with a mol. 11209750 8 67 gly calnexin/calreticulin 1265:1285 arg1 not the calnexin/calreticulin UDP calnexin not the calnexin/calreticulin UDP OGER calnexin P35564 We conclude that ER mannosidases and proteasome activities, but not glucose trimming (and therefore, most likely not the calnexin/calreticulin UDP:glucose glycoprotein glucosyl transferase cycle), are essential for ER-associated degradation (ERAD) of soluble glycoproteins. 6236213 10 17 gly contained 1322:1330 arg1 The accumulated cathepsin D precursor AND mannose 6-phosphate residues The accumulated cathepsin D precursor mannose 6-phosphate residues PUBTATOR cathepsin D precursor 1509 The accumulated cathepsin D precursor contained neither mannose 6-phosphate residues nor complex type oligosaccharides, which are formed in the cis and trans aspects of the Golgi complex. 6236213 10 17 gly contained 1322:1330 arg1 The accumulated cathepsin D precursor AND complex type oligosaccharides The accumulated cathepsin D precursor complex type oligosaccharides PUBTATOR cathepsin D precursor 1509 The accumulated cathepsin D precursor contained neither mannose 6-phosphate residues nor complex type oligosaccharides, which are formed in the cis and trans aspects of the Golgi complex. 2917524 7 53 gly nonglycosylated 963:977 arg1 nonglycosylated ovine PRL nonglycosylated ovine PRL PUBTATOR PRL 5617 G1-hPRL had only about one fourth the activity of the reference standard (nonglycosylated ovine PRL, 35 IU/mg). 26191964 3 92 gly glycosylation 518:530 arg1 IgG IgG Cterm IgG Matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS) shows potential for the site-specific glycosylation analysis of IgG at the glycopeptide level. 8183238 12 100 gly deglycosylated 2376:2389 arg1 The CCK-AR The CCK-AR PUBTATOR CCK-AR 24889 The CCK-AR, which has three potential sites for N-glycosylation on the amino-terminal extracellular domain and one on the second extracytoplasmic loop, was deglycosylated to a 42-kDa peptide. 23376777 8 23 gly Deglycosylated 1486:1499 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 Deglycosylated forms of KCC4 also demonstrated decreased tumor formation and lung colonization in mouse xenografts. 21738755 3 82 gly DC-SIGN/E-glycoprotein 427:448 arg1 DC-SIGN/E-glycoprotein DC-SIGN/E-glycoprotein PUBTATOR DC-SIGN 30835 Thus, the DC-SIGN/E-glycoprotein interaction can be considered as an important target for inhibitors of viral replication. 12877809 10 103 part_of Asn342 1579:1584 arg1 the tree shrew CETP protein CETP protein Asn342 PUBTATOR AminoAcid CETP protein 100327267 Asn342 There is a deletion of an N-linked glycosylation site at Asn342 in the tree shrew CETP protein that may participate in the removal of peripheral cholesterol and cholesteryl ester by increasing its activity of transferring cholesteryl ester. 11689624 15 73 gly moieties 2603:2610 arg1 gp41 gp41 moieties Cterm gp41 The viral variants described in this report should prove useful for investigation of the contribution of carbohydrate moieties on gp41 to recognition by antibodies, shielding from antibody-mediated neutralization, and structure-function relationships. 8280475 3 40 gly glycosylation 467:479 arg1 gp160 gp160 PUBTATOR gp160 2028 No significant differences in the kinetics of synthesis of gp160, processing into gp120 and gp41 proteins, N-linked glycosylation, or release of gp120 into the medium were noted with the different envelope proteins. 8280475 3 40 gly glycosylation 467:479 arg1 gp120 gp120 PUBTATOR gp120 3700 No significant differences in the kinetics of synthesis of gp160, processing into gp120 and gp41 proteins, N-linked glycosylation, or release of gp120 into the medium were noted with the different envelope proteins. 25707740 4 12 gly glycoprotein 452:463 arg1 hCG hCG OGER hCG Specific to humans, hCG is a complex glycoprotein composed of two glycosylated subunits. 20561589 1 65 gly glycoprotein 125:136 arg1 CD83 CD83 PUBTATOR CD83 9308 CD83 is a transmembrane glycoprotein of the immunoglobulin (Ig) superfamily and a surface marker for fully matured dendritic cells (DCs) in humans and mice. 1482344 5 14 gly BuChE 829:833 arg1 the known N-linked oligosaccharides BuChE the known N-linked oligosaccharides PUBTATOR BuChE 590 All nine of the known N-linked oligosaccharides of BuChE are predicted to occur away from the putative active site channel and most are located on one face of the monomer. 25694612 6 12 gly IgG-BCR 958:964 arg1 the core fucosylation BCR the core fucosylation OGER BCR Q6PAJ1 Our results clearly suggest an unexpected mode of BCR function, in which the core fucosylation of IgG-BCR mediates Ag recognition and, concomitantly, cell signal transduction via BCR and Ab production. 25694612 6 44 gly fucosylation 942:953 arg1 IgG-BCR IgG-BCR OGER BCR Q6PAJ1 Our results clearly suggest an unexpected mode of BCR function, in which the core fucosylation of IgG-BCR mediates Ag recognition and, concomitantly, cell signal transduction via BCR and Ab production. 8386874 6 4 gly glycosylated 1072:1083 arg1 glycosylated L1 glycosylated L1 Cterm L1 We conclude that glycosylated L1 is unlikely to be an important component of the papillomavirus virion, a finding of importance for the design of papillomavirus-specific vaccines. 30227620 5 23 gly hyposialylated 755:768 arg1 hyposialylated Tg hyposialylated Tg Cterm Tg 7038 N-oligosaccharides play a role in Tg transport into the follicular lumen, where thyroid hormones are produced, and into thyrocytes, where hyposialylated Tg is degraded. 2346009 1 1 gly glycosylation 150:162 arg1 human plasma albumin human plasma albumin OGER albumin P02768 The nonenzymatic glycosylation of human plasma albumin by glucose, fructose, xylose, glucose-6-P, fructose-6-P, 6-PP was studied in vitro. 12039072 11 34 gly O-glycosylation 1884:1898 arg1 SHBG binding SHBG PUBTATOR SHBG 6462 These data suggest that O-glycosylation of SHBG is essential for SHBG binding to a membrane receptor that is responsible for inhibiting the estradiol-induced proliferation of MCF-7 breast cancer cells. 26013384 0 10 part_of Ser126 59:64 arg1 Human Recombinant Erythropoietin Erythropoietin Ser126 PUBTATOR AminoAcid Erythropoietin 2056 Ser126 Structural Identification of a Non-Glycosylated Variant at Ser126 for O-Glycosylation Site from EPO BRP, Human Recombinant Erythropoietin by LC/MS Analysis. 2971395 5 24 gly oligosaccharides 812:827 arg1 mature hexosaminidase hexosaminidase oligosaccharides OGER hexosaminidase In order to define the structure and placement of the oligosaccharides in mature hexosaminidase and thus identify candidate mannose 6-phosphate recipient sites, the major tryptic/chymotryptic glycopeptides from each isozyme were purified by reverse-phase high-performance liquid chromatography. 17324955 10 2 gly ACIII 1690:1694 arg1 N-glycans ACIII N-glycans PUBTATOR ACIII 104111 Thus, the structure of N-glycans of ACIII regulates its enzymatic activity and downstream signaling. 3011898 1 60 gly contain 221:227 arg1 FPCR AND at least two asparagine-linked oligosaccharide chains FPCR at least two asparagine-linked oligosaccharide chains Cterm FPCR We previously showed that formyl peptide chemotactic receptors (FPCR) of human phagocytic cells contain at least two asparagine-linked oligosaccharide chains located at the distal end of the receptor. 28710799 0 50 gly glycosylation 30:42 arg1 VDR VDR PUBTATOR VDR 7421 High glucose induces O-GlcNAc glycosylation of the vitamin D receptor (VDR) in THP1 cells and in human macrophages derived from monocytes. 28710799 0 50 gly glycosylation 30:42 arg1 the vitamin D receptor the vitamin D receptor PUBTATOR vitamin D receptor 7421 High glucose induces O-GlcNAc glycosylation of the vitamin D receptor (VDR) in THP1 cells and in human macrophages derived from monocytes. 28710799 0 58 gly receptor 61:68 arg1 O-GlcNAc glycosylation vitamin D receptor O-GlcNAc glycosylation PUBTATOR vitamin D receptor 7421 High glucose induces O-GlcNAc glycosylation of the vitamin D receptor (VDR) in THP1 cells and in human macrophages derived from monocytes. 23527139 0 87 gly glycoprotein 46:57 arg1 dengue envelope glycoprotein dengue envelope glycoprotein PUBTATOR envelope glycoprotein 100616444 Interaction and inhibition of dengue envelope glycoprotein with mammalian receptor DC-sign, an in-silico approach. 3680284 2 31 gly glycosylation 128:140 arg1 Cu-Zn-superoxide dismutase Cu-Zn-superoxide dismutase PUBTATOR Cu-Zn-superoxide dismutase 6647 The nonenzymatic glycosylation (glycation) of Cu-Zn-superoxide dismutase led to gradual inactivation of the enzyme (Arai, K. Iizuka, S., Tada, Y., Oikawa, K., and Taniguchi, N. (1987) Biochim. 28974560 7 57 gly glycosylation 1119:1131 arg1 STAT5 STAT5 PUBTATOR STAT5 6776 STAT5 is an important mediator of the EMT process and loss of SR-BI resulted in decreased glycosylation, reduced DNA binding, and target gene expression of STAT5. 25546301 1 1 gly gp120 281:285 arg1 the 15 N-linked glycans gp120 the 15 N-linked glycans PUBTATOR gp120 155971 The crystal structure of a fully glycosylated HIV-1 gp120 core in complex with CD4 receptor and Fab 17b at 4.5-Å resolution reveals 9 of the 15 N-linked glycans of core gp120 to be partially ordered. 24824609 3 41 gly glycosylation 647:659 arg1 antithrombin antithrombin PUBTATOR antithrombin 462 OBJECTIVES: To evaluate the effects of different natural pleiotropic mutations on the glycosylation of antithrombin and their functional effects. 26059044 9 20 gly IL-22 1367:1371 arg1 the atypical N-glycan composition IL-22 the atypical N-glycan composition PUBTATOR IL-22 50616 However, we do show that the presence of a N-glycan on Asn54 contributes to the atypical N-glycan composition of plant-produced IL-22 and influences the N-glycan composition of N-glycans on other positions. 24291635 2 26 gly Fully-glycosylated 302:319 arg1 Fully-glycosylated hFSH Fully-glycosylated hFSH OGER hFSH Fully-glycosylated hFSH(24) was prepared by combining the fully-glycosylated FSHβ(24) variant with hCGα and isolating the heterodimer. 24291635 2 26 gly Fully-glycosylated 302:319 arg1 24 24 Cterm 24 Fully-glycosylated hFSH(24) was prepared by combining the fully-glycosylated FSHβ(24) variant with hCGα and isolating the heterodimer. 12573291 7 52 gly nonglycosylated 1402:1416 arg1 BACE BACE PUBTATOR BACE 23621 These data demonstrate that the nonglycosylated, soluble catalytic domain of BACE faithfully reflects the ligand binding properties of the full-length mature enzyme in its natural membrane environment. 24291635 0 38 gly Hypo-glycosylated 0:16 arg1 hFSH(21/18) hFSH(21/18) OGER hFSH Hypo-glycosylated human follicle-stimulating hormone (hFSH(21/18)) is much more active in vitro than fully-glycosylated hFSH (hFSH(24)). 24291635 0 47 gly fully-glycosylated 101:118 arg1 hFSH(24) hFSH OGER hFSH Hypo-glycosylated human follicle-stimulating hormone (hFSH(21/18)) is much more active in vitro than fully-glycosylated hFSH (hFSH(24)). 26121645 7 100 gly glycoprotein 1495:1506 arg1 envelope glycoprotein envelope glycoprotein PUBTATOR envelope glycoprotein 100616444 The generated HIV-1NL4.3 mutants were subjected to an array of assays, determining the envelope glycoprotein levels in mutant viral particles, their infectivity and the capture and transmission efficiencies of mutant virus particles by DC-SIGN. 25213400 3 21 part_of Asn 286:288 arg1 MOG MOG Asn PUBTATOR SpecificSite MOG 4340 Asn(31) Other myelin glycoproteins, also with a single glycosylation site (PMP22 at Asn(36) , MOG at Asn(31) ), bind only one glycan. 25213400 3 73 part_of Asn 269:271 arg1 PMP22 PMP22 Asn PUBTATOR SpecificSite PMP22 5376 Asn(36) Other myelin glycoproteins, also with a single glycosylation site (PMP22 at Asn(36) , MOG at Asn(31) ), bind only one glycan. 24724053 5 15 gly EMT 760:762 arg1 all hallmarks EMT all hallmarks OGER EMT O75751 Current scientific methodology enables all hallmarks of EMT to be monitored in vitro and this experimental model has been extensively used in oncology research during the last 10 years. 23523791 5 49 gly glycosylation 775:787 arg1 Mef2D Mef2D PUBTATOR Mef2D 17261 A significant decrease in the glycosylation of Mef2D was observed in response to myogenic stimulus in C2C12 cells. 10550318 7 60 gly sialoglycoprotein 975:991 arg1 mVAP-1 mVAP-1 PUBTATOR mVAP-1 11754 mVAP-1 is a 220-kd homodimeric sialoglycoprotein that displays cell-type-specific differences in glycosylation. 8148809 0 52 gly glycosylated 27:38 arg1 glycosylated human serum albumin glycosylated human serum albumin PUBTATOR serum albumin 213 Drug binding properties of glycosylated human serum albumin as measured by fluorescence and circular dichroism. 6310544 0 63 gly glycosylation 33:45 arg1 glycoprotein gp71A glycoprotein gp71A Cterm gp71A Complete amino acid sequence and glycosylation sites of glycoprotein gp71A of Friend murine leukemia virus. 16014566 6 89 gly glycosylation 1277:1289 arg1 human GPVI human GPVI PUBTATOR GPVI 51206 These findings indicate that N-linked glycosylation at N92 in human GPVI is not required for surface expression, but contributes to maximal adhesion to type I collagen, CRP and, to a lesser extent, CVX. 23376777 4 69 gly glycosylation 711:723 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 We showed that triple (N312/331/344/Q) and quadruple (N312/331/344/360/Q) mutations of N-linked glycosylation sites disrupt the N-linked glycosylation of KCC4, resulting in the accumulation of KCC4, predominantly in the endoplasmic reticulum (ER) and not at the cell surface. 15814824 2 91 gly MUC1 404:407 arg1 the repeat domain MUC1 the repeat domain PUBTATOR MUC1 4582 We recently showed that clusters of sequence-variant repeats are interspersed in the repeat domain of MUC1 at high frequency, which should contribute to the structural and immunological features of the mucin. 18235976 0 79 gly N-glycosylation 0:14 arg1 E-cadherin E-cadherin PUBTATOR E-cadherin 999 N-glycosylation at Asn residues 554 and 566 of E-cadherin affects cell cycle progression through extracellular signal-regulated protein kinase signaling pathway. 16227292 10 51 gly glycans 1538:1544 arg1 Gc Gc glycans Cterm Gc The viability of these recombinant viruses and analysis of growth kinetics indicates that the glycans on Gc are not essential for BUN replication, but they do contribute to the efficiency of virus infection. 9287313 4 24 gly glycoforms 758:767 arg1 human CD69 glycoforms human CD69 glycoforms PUBTATOR CD69 969 In the current report we show that human CD69 glycoforms are generated before the egress of CD69 proteins from the endoplasmic reticulum to the Golgi and are synthesized under conditions where Golgi processing is inhibited, effectively ruling out the possibility that CD69 heterogeneity results from the differential processing of a single glycosylation site in the Golgi complex. 7584619 0 26 gly nonglycosylated 34:48 arg1 nonglycosylated human transferrin nonglycosylated human transferrin PUBTATOR transferrin 7018 Optimized bacterial production of nonglycosylated human transferrin and its half-molecules. 7687365 0 37 gly glycoprotein 61:72 arg1 myelin/oligodendrocyte glycoprotein myelin/oligodendrocyte glycoprotein PUBTATOR myelin/oligodendrocyte glycoprotein 421050 Cloning and cDNA sequence analysis of myelin/oligodendrocyte glycoprotein: a novel member of the immunoglobulin gene superfamily. 14691230 6 37 gly glycosylation 1065:1077 arg1 DPPIV DPPIV PUBTATOR DPPIV 1803 Crystallographic and biochemical data demonstrate that N-linked glycosylation of DPPIV does not contribute significantly to its peptidase activity. 12565836 7 36 gly N-glycosylation 968:982 arg1 FPR FPR PUBTATOR FPR 2357 Our data indicate that N-glycosylation of N-terminal Asn4 and Asn10 but not of Asn179 in the second extracellular loop is essential for proper folding and, hence, function of FPR. 27175940 2 14 part_of p27 474:476 arg1 Thr157 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser2 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser106 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser2 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser106 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Ser106 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 27175940 2 14 part_of p27 474:476 arg1 Thr198 p27 Ser2, Ser106, Ser110, Thr157, and Thr198 PUBTATOR AminoAcid p27 3429 Ser2, Ser106, Ser110, Thr157, and Thr198 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 15026024 5 28 gly G-CSF 1127:1131 arg1 the carbohydrate residues G-CSF the carbohydrate residues PUBTATOR G-CSF 1440 We used an enzymatic approach to remove the carbohydrate residues from glycosylated G-CSF and tested this material for its stability in serum. 15026024 5 45 gly glycosylated 1114:1125 arg1 glycosylated G-CSF glycosylated G-CSF PUBTATOR G-CSF 1440 We used an enzymatic approach to remove the carbohydrate residues from glycosylated G-CSF and tested this material for its stability in serum. 18533687 8 9 gly N-glycosylation 1305:1319 arg1 RXFP1 RXFP1 PUBTATOR RXFP1 59350 All of the potential N-glycosylation sites of RXFP1 were utilized in HEK-293T cells, and importantly, disruption of glycosylation at individual or combinations of double and triple sites had little effect on relaxin binding. 18642129 12 16 part_of position 1702:1709 arg1 the light chain chain position OGER SpecificSite chain P08709 position Ser(60) and Ser(52) Last, MS and MS/MS analysis revealed that FVII is O-glycosylated on the light chain at position Ser(60) and Ser(52) which are modified by oligosaccharide structures such as fucose and Glc(Xyl)(0-1-2), respectively. 7642555 6 17 gly s 607:607 arg1 c-Myc c-Myc s PUBTATOR c-Myc 4609 In this paper, we identified the O-GlcNAc attachment site(s) on c-Myc. 20686018 5 75 gly glycoprotein 1103:1114 arg1 myelin-associated glycoprotein myelin-associated glycoprotein PUBTATOR myelin-associated glycoprotein 4099 In contrast, the mutations had no effect on viral entry in CHO cells expressing known host cell receptors for HSV-1 gD, viral entry in HL60 cells expressing myelin-associated glycoprotein (MAG) (another HSV-1 gB receptor), viral attachment to heparan sulfate, and viral replication in PILRα-negative cells. 20686018 5 75 gly glycoprotein 1103:1114 arg1 MAG MAG PUBTATOR MAG 4099 In contrast, the mutations had no effect on viral entry in CHO cells expressing known host cell receptors for HSV-1 gD, viral entry in HL60 cells expressing myelin-associated glycoprotein (MAG) (another HSV-1 gB receptor), viral attachment to heparan sulfate, and viral replication in PILRα-negative cells. 29187599 3 84 part_of sites 367:371 arg1 rhDAO rhDAO sites OGER SpecificSite rhDAO O35078 sites Asn-168, Asn-538, and Asn-745 We recently reported that the N-glycosylation sites Asn-168, Asn-538, and Asn-745 in recombinant hDAO (rhDAO) carry complex-type glycans, whereas Asn-110 carries only mammalian-atypical oligomannosidic glycans. 29187599 3 84 part_of sites 367:371 arg1 recombinant hDAO hDAO sites OGER SpecificSite hDAO P14920 sites Asn-168, Asn-538, and Asn-745 We recently reported that the N-glycosylation sites Asn-168, Asn-538, and Asn-745 in recombinant hDAO (rhDAO) carry complex-type glycans, whereas Asn-110 carries only mammalian-atypical oligomannosidic glycans. 24780636 1 1 gly possesses 127:135 arg1 Transferrin AND N-glycans Transferrin N-glycans PUBTATOR Transferrin 7018 Transferrin is an iron-transport protein which possesses N-glycans at Asn432 and Asn630 in humans. 7831351 1 13 gly glycoprotein 172:183 arg1 carboxyl ester lipase carboxyl ester lipase OGER Pancreatic carboxyl ester lipase P07882 Pancreatic carboxyl ester lipase is a glycoprotein that requires millimolar concentrations of trihydroxy bile salts, such as cholate, for maximal catalytic activity against cholesteryl esters and triglycerides. 21511948 10 9 gly glycosylated 1358:1369 arg1 WT RAGE WT RAGE PUBTATOR WT RAGE 177 Using mass spectrometry analysis, we found that Asn(81) may or may not be glycosylated in WT RAGE, whereas in G82S RAGE, Asn(81) is always glycosylated. 21511948 10 24 gly glycosylated 1423:1434 arg1 G82S RAGE G82S RAGE PUBTATOR G82S RAGE 177 Using mass spectrometry analysis, we found that Asn(81) may or may not be glycosylated in WT RAGE, whereas in G82S RAGE, Asn(81) is always glycosylated. 7613486 1 29 gly glycosylation 193:205 arg1 the insulin receptor the insulin receptor PUBTATOR insulin receptor 3643 Asparagine-linked glycosylation of the insulin receptor is required for complete biosynthesis and acquisition of function. 1703212 1 43 gly glycoprotein 287:298 arg1 gp 120 gp 120 PUBTATOR gp 120 155971 Antibodies raised to an overlapping series of peptides following the amino acid sequence of the external envelope glycoprotein (gp 120) of human immunodeficiency virus type 1 (HIV-1) recognize eight regions in recombinant gp 120 molecules. 27480168 9 23 gly glycosylation 1664:1676 arg1 hENT1 hENT1 OGER hENT1 Q99808 Based on these data we propose that glycosylation at N48 is critical for the localization, function and oligomerization of hENT1. 27638310 9 18 gly rrhGM-CSF 1338:1346 arg1 O-glycans CSF O-glycans OGER CSF These findings indicate that O-glycans of rrhGM-CSF are essential for maintaining its structural stability and result in an extended in vivo half-life, but without affecting its biological function. 19158400 4 19 gly glycosylation 727:739 arg1 AC8 AC8 PUBTATOR AC8 114 In the present study, we show that an intact leucine zipper motif is required for the efficient N-linked glycosylation of AC8, and that this N-linked glycosylation is important to target AC8 into lipid rafts. 25965797 2 30 gly glycoprotein 207:218 arg1 HSV-2 glycoprotein B HSV-2 glycoprotein B Cterm HSV-2 glycoprotein B HSV-2 glycoprotein B together with glycoproteins D, H and L are indispensable for viral entry, of which gB, as a class III fusogen, plays an essential role. 29416877 2 39 gly hypoglycosylated 339:354 arg1 hypo-BSP hypo-BSP Cterm hypo-BSP 3381 Here, we report on the diagnostic and therapeutic properties of IDK1, an antibody against tumour associated, hypoglycosylated bone sialoprotein (hypo-BSP). 29416877 2 39 gly hypoglycosylated 339:354 arg1 hypoglycosylated bone sialoprotein hypoglycosylated bone sialoprotein PUBTATOR bone sialoprotein 3381 Here, we report on the diagnostic and therapeutic properties of IDK1, an antibody against tumour associated, hypoglycosylated bone sialoprotein (hypo-BSP). 24085305 11 61 gly glycosylated 1774:1785 arg1 optimally glycosylated LILRA3 optimally glycosylated LILRA3 PUBTATOR LILRA3 11026 Binding to monocytes was partially blocked by β-lactose, indicating that optimally glycosylated LILRA3 might be critical for ligand binding and function. 18201245 8 37 gly N-glycosylation 1342:1356 arg1 the ER the ER Cterm ER, S4 In some cell types, much of the S4 glycosylation, but not that of S1, was resistant to endoglycosidase H, suggesting that, subsequent to core N-glycosylation in the ER, S4 was transported anterograde to the Golgi, where further glycosylation occurred. 27177499 14 21 gly receptor 2010:2017 arg1 a LH/CG-R independent signalling pathway TGFß a LH/CG-R independent signalling pathway Cterm TGFß By contrast, hCG-H stimulates trophoblast invasion and angiogenesis by interacting with the TGFß receptor in a LH/CG-R independent signalling pathway. 29784879 7 64 gly EDEM3 1287:1291 arg1 the mannose-trimming activity EDEM3 the mannose-trimming activity PUBTATOR EDEM3 80267 In a defined in vitro system consisting of recombinant proteins purified from HEK293 cells, the mannose-trimming activity of EDEM3 toward the model misfolded substrate, the glycoprotein T-cell receptor α locus (TCRα), was reconstituted only when ERp46 had established a covalent interaction with EDEM3. 10353820 4 54 gly fucosylated 721:731 arg1 both nonglycosylated and fucosylated FVII EGF-1 both nonglycosylated and fucosylated FVII EGF-1 OGER FVII EGF-1 P08709 Distance and dihedral constraints derived from NMR data were used to determine the solution structures of both nonglycosylated and fucosylated FVII EGF-1 in the presence of CaCl2. 10353820 4 76 gly nonglycosylated 701:715 arg1 both nonglycosylated and fucosylated FVII EGF-1 both nonglycosylated and fucosylated FVII EGF-1 OGER FVII EGF-1 P08709 Distance and dihedral constraints derived from NMR data were used to determine the solution structures of both nonglycosylated and fucosylated FVII EGF-1 in the presence of CaCl2. 1904027 7 62 gly glycosylated 1033:1044 arg1 glycosylated active renin glycosylated active renin OGER renin P08424 The comparison of glycosylated and unglycosylated renin expression showed a diminished secretion of glycosylated active renin. 10622399 0 122 gly glycosylation 13:25 arg1 recombinant human sex hormone-binding globulin recombinant human sex hormone-binding globulin PUBTATOR sex hormone-binding globulin 6462 Influence of glycosylation on the clearance of recombinant human sex hormone-binding globulin from rabbit blood. 24425712 3 53 gly α2,3-sialyltransferase 553:574 arg1 ST3Gal-IV α2,3-sialyltransferase IV ST3Gal-IV PUBTATOR α2,3-sialyltransferase IV 20443 OBJECTIVE: Because the chemokine receptors for Ccl5 and Ccl2 are important in atherogenic recruitment of neutrophils and monocytes, we here investigated the role of α2,3-sialyltransferase IV (ST3Gal-IV) in Ccl5- and Ccl2-mediated myeloid cell arrest and further studied its relevance in a mouse model of atherosclerosis. 22243251 12 5 gly has 1422:1424 arg1 Mouse KLK4 AND NA3F N-glycan cores Mouse KLK4 NA3F N-glycan cores PUBTATOR Mouse KLK4 56640 Mouse KLK4 has NA2, NA2F, and NA3F N-glycan cores with no, or with one or two sialic acids. 12930842 6 54 gly IFN-alpha 882:890 arg1 IFN-alpha(psi2) transcripts IFN-alpha IFN-alpha(psi2) transcripts PUBTATOR IFN-alpha 111654 IFN-alpha(psi2) transcripts are the most abundant IFN-alpha transcripts detected in several mouse organs in the absence of viral infection. 8292828 3 111 gly glycoprotein 607:618 arg1 rat CD24 rat CD24 PUBTATOR CD24 25145 Rat CD24 cDNA is homologous to murine and human CD24 gene with respect to the structure of signal peptide, N-glycosylation sites, and possible glycosyl phosphatidylinositol (GPI) linker attaching site, suggesting that rat CD24 is a transducing glycoprotein anchoring membrane via GPI linker. 9930668 4 60 gly present 935:941 arg2 the HVAP-1 AND all components the HVAP-1 all components OGER HVAP-1 Q16853 The structural model of the catalytic D4 domain of HVAP-1 reveals that all components necessary for enzymatic monoamine oxidase activity are indeed present within the HVAP-1 and pinpoints residues that may be key to substrate entry through a channel to the active site and residues likely to be involved in substrate specificity as well as structural features critical to dimer formation. 20408681 5 0 gly chains 701:706 arg1 rLF rLF chains OGER rLF Each of the three attached glycan chains on rLF contains seven to eight sugar groups. 19874459 11 8 gly glycosylation 1624:1636 arg1 VWF functions VWF functions PUBTATOR VWF 7450 In conjunction with current models explaining VWF activity, knowledge of the complete O-glycome will facilitate research aimed at providing a better understanding of the influence of glycosylation on VWF functions. 8169523 1 87 gly glycoprotein 109:120 arg1 Apolipoprotein J Apolipoprotein J PUBTATOR Apolipoprotein J 1191 Apolipoprotein J (apoJ), a glycoprotein associated with subclasses of plasma high density lipoproteins (HDL), was found to accumulate in aortic lesions in a human subject with transplantation-associated arteriosclerosis and in mice fed a high-fat atherogenic diet. 8509412 10 57 gly unglycosylated 1537:1550 arg1 the unglycosylated mutated transferrin receptor the unglycosylated mutated transferrin receptor PUBTATOR transferrin receptor 7018 Addition of a new glycosylation site in the C-terminal region of the unglycosylated mutated transferrin receptor restores the cell surface localization and the transferrin binding of the transferrin receptor, indicating that glycosylation in this region is critical for the correct transport of this receptor to the cell surface. 7589110 9 43 gly deglycosylated 1223:1236 arg1 E-selectin E-selectin PUBTATOR E-selectin 6401 The deglycosylated forms of E-selectin were also found to be fully capable of mediating adhesion of HT-29 cells in vitro. 9278435 3 38 gly alpha-L-iduronidase 544:562 arg1 the oligosaccharides alpha-L-iduronidase the oligosaccharides PUBTATOR alpha-L-iduronidase 100767663 Because of the importance of carbohydrate residues for endocytosis and lysosomal targeting, we examined the oligosaccharides of recombinant alpha-L-iduronidase at each of its six N-glycosylation sites. 8357534 4 44 gly contain 715:721 arg1 E-3 AND exclusively truncated, oligomannose-type chains E-3 and C-3 positions exclusively truncated, oligomannose-type chains PUBTATOR E-3 and C-3 26765 positions In contrast, the isoforms E-3 and C-3, the major forms of elastase and cathepsin G respectively, contain exclusively truncated, oligomannose-type chains at the same positions in the sequence of each protein. 10622399 1 2 gly glycoprotein 168:179 arg1 Human sex hormone-binding globulin Human sex hormone-binding globulin PUBTATOR Human sex hormone-binding globulin 100009224 Human sex hormone-binding globulin (hSHBG) is a plasma glycoprotein that binds sex steroids with high affinity. 15316006 6 2 gly beta1 804:808 arg1 all effects beta1 all effects PUBTATOR beta1 3779 Consistent with this, mutation of beta1 N-glycosylation sites abolished all effects of beta1 on channel gating. 24014025 4 31 gly N-glycosylated 777:790 arg1 secreted LOXL2 secreted LOXL2 PUBTATOR LOXL2 4017 We discovered that secreted LOXL2 (~100-kDa) is N-glycosylated at Asn-455 and Asn-644, whereas intracellular LOXL2 (~75-kDa) is nonglycosylated and N-terminally processed, and is primarily associated with the nucleus. 27465341 3 59 gly VP 569:570 arg1 all VP all Cterm VP Our results showed that the VP proteins of CVA6 were all of hydrophilic nature, contained phosphorylation and glycosylation sites and harbored no signal peptide sequences and acetylation sites. 15576633 3 17 gly nonglycosylated 791:805 arg1 a nonglycosylated hOAT4 a nonglycosylated hOAT4 PUBTATOR hOAT4 55867 We showed that both the disruption of the glycosylation sites by mutagenesis and the inhibition of glycosylation by tunicamycin treatment resulted in a nonglycosylated hOAT4, which was unable to target to the cell surface. 19501045 7 26 gly unglycosylated 788:801 arg1 the unglycosylated gp130 the unglycosylated gp130 PUBTATOR gp130 16195 Although the unglycosylated gp130 was found to be expressed on the cell surface, it could not form a heterodimer with leukemia inhibitory factor receptor. 26121645 5 73 gly N-glycans 985:993 arg1 glycoprotein E1 E1 N-glycans Cterm E1 Such a pronounced co-localization of disulphide bridges and N-glycans was also found for the N-glycans on glycoprotein E1 of the hepatitis C virus (HCV) but not for other heavily glycosylated proteins such as E2 from HCV and the surface GP from Ebola virus. 3384816 2 105 gly glycoprotein 108:119 arg1 ascites sialoglycoprotein-1 ascites sialoglycoprotein-1 OGER ascites sialoglycoprotein Q63661 The major cell surface glycoprotein (ascites sialoglycoprotein-1 (ASGP-1] of ascites 13762 rat mammary tumor cells is a large (Mr greater than 500,000), highly glycosylated sialomucin which is present in great abundance (greater than 0.5% of total cell protein). 12765790 0 49 gly polysialylated 56:69 arg1 bovine polysialylated NCAM bovine polysialylated NCAM PUBTATOR NCAM 17967 Localization of defined carbohydrate epitopes in bovine polysialylated NCAM. 6853480 9 9 gly glycosylation 1222:1234 arg1 human serum albumin human serum albumin OGER albumin P02768 Thus, lysine-525 is the predominant site of nonenzymatic glycosylation of human serum albumin in vivo. 29441788 9 20 gly rhAFM 1459:1463 arg1 various partially or nonglycosylated forms rhAFM various partially or nonglycosylated forms OGER rhAFM P36953 Subsequent results showed sufficient expression of various partially or nonglycosylated forms of rhAFM in HEK293T and CHO cells and revealed that glycosylation is not necessary for expression and secretion. 7734846 5 15 gly glycosylation 782:794 arg1 human glycophorin A human glycophorin A PUBTATOR glycophorin A 2993 The glycosylation status of 15 of 17 potential O-glycosylation sites in the B variant was accurately predicted using the four peptide motifis previously proposed for the glycosylation of human glycophorin A (Pisano, A., Redmond, J.W., Williams, K.L. and Gooley, A.A., Glycobiology, 3, 429-435, 1993), provided one additional assumption is made concerning an inhibitory role for a nearby Ile. 15150274 5 59 gly carried 883:889 arg1 human truncated Duox2 AND only high mannose-type sugar chains human truncated Duox2 only high mannose-type sugar chains PUBTATOR Duox2 50506 Carbohydrate content analysis revealed that complex type-specific Golgi apparatus (GA) oligosaccharides were present on pig Duox2-Q686X, whereas human truncated Duox2 carried only high mannose-type sugar chains characteristic of the ER. 15150274 5 89 gly present 825:831 arg1 pig Duox2-Q686X AND complex type-specific Golgi apparatus (GA) oligosaccharides Duox2 complex type-specific Golgi apparatus (GA) oligosaccharides PUBTATOR Duox2 397060 Carbohydrate content analysis revealed that complex type-specific Golgi apparatus (GA) oligosaccharides were present on pig Duox2-Q686X, whereas human truncated Duox2 carried only high mannose-type sugar chains characteristic of the ER. 15047148 3 40 gly hypoglycosylated 435:450 arg1 hypoglycosylated wildtype CD28 hypoglycosylated wildtype CD28 PUBTATOR CD28 940 In this report, we have investigated the function of hypoglycosylated wildtype CD28 and its splice variant, CD28i. 27681177 4 52 gly glycosylated 783:794 arg1 human SLC26A6 human SLC26A6 PUBTATOR SLC26A6 65010 Through a series of enzymatic deglycosylation studies we confirmed that endogenously expressed mouse and human SLC26A6 are indeed glycosylated, that the oligosaccharides are principally attached via N-glycosidic linkage, and that there are tissue-specific differences in glycosylation. 19038921 5 29 gly glycosylated 955:966 arg1 the endogenous bovine alpha-LA the endogenous bovine alpha-LA PUBTATOR alpha-LA 3906 Additionally, no N-glycosylation was found in the recombinant human alpha-LA, whereas the endogenous bovine alpha-LA was glycosylated at the unusual site (71)Asn-Ile-(73)Cys. 19038921 5 47 gly N-glycosylation 851:865 arg1 the recombinant human alpha-LA the recombinant human alpha-LA PUBTATOR alpha-LA 3906 Additionally, no N-glycosylation was found in the recombinant human alpha-LA, whereas the endogenous bovine alpha-LA was glycosylated at the unusual site (71)Asn-Ile-(73)Cys. 9820205 3 37 gly glycoproteins 431:443 arg1 rZP2 rZP2 PUBTATOR rZP2 81828 The ZP in this species, like the mouse, hamster, and human, was found to contain three glycoproteins, namely rZP1, rZP2, and rZP3 (Araki et al. [1992] Biol Reprod 46:912-919). 9820205 3 37 gly glycoproteins 431:443 arg1 rZP1 rZP1 PUBTATOR rZP1 85271 The ZP in this species, like the mouse, hamster, and human, was found to contain three glycoproteins, namely rZP1, rZP2, and rZP3 (Araki et al. [1992] Biol Reprod 46:912-919). 9820205 3 37 gly glycoproteins 431:443 arg1 rZP3 rZP3 PUBTATOR rZP3 114639 The ZP in this species, like the mouse, hamster, and human, was found to contain three glycoproteins, namely rZP1, rZP2, and rZP3 (Araki et al. [1992] Biol Reprod 46:912-919). 9267001 1 44 gly glycoprotein 290:301 arg1 glycoprotein B glycoprotein B Cterm glycoprotein B To better understand host immunological responses to viral glycoproteins, the glycoprotein B (gB) gene of RhCMV was molecularly cloned, sequenced and characterized. 1587794 6 14 gly N-glycosylation 832:846 arg1 the native MMGL the native MMGL PUBTATOR MMGL 64195 Two N-glycosylation sites were found in the extracellular region of MMGL, corresponding to the heavy N-glycosylation in the native MMGL. 29626154 7 79 gly O-glycosylation 1267:1281 arg1 OPN OPN PUBTATOR OPN 6696 An adhesion assay using function-blocking antibodies against αvβ3 and β1 integrins, as well as αvβ3 integrin-overexpressing A549 cells, revealed that site-specific O-glycosylation affected the association of OPN with the two integrins. 17152094 4 42 gly glycoprotein 530:541 arg1 AGP AGP Cterm AGP We detected a typical protein pattern for alpha1-acid glycoprotein (AGP) and transferrin (Trf) that are markers for CDG. 19508227 0 14 gly Glycosylation 0:12 arg1 tetraspanin Tspan-1 tetraspanin Tspan-1 PUBTATOR Tspan-1 10103 Glycosylation of tetraspanin Tspan-1 at four distinct sites promotes its transition through the endoplasmic reticulum. 9774483 3 87 gly attached 659:666 arg1 NCAM AND N-glycans NCAM N-glycans PUBTATOR NCAM 4684 We first examined whether PST and STX differ in the requirement of sialic acid and core structures of N-glycans attached to NCAM. 7685769 0 25 gly glycoforms 127:136 arg1 recombinant human granulocyte colony-stimulating factor recombinant human granulocyte colony-stimulating factor OGER granulocyte colony-stimulating factor P09919 Glycosidase digestion, electrophoresis and chromatographic analysis of recombinant human granulocyte colony-stimulating factor glycoforms produced in Chinese hamster ovary cells. 8621728 0 55 gly glycoprotein 59:70 arg1 recombinant P-selectin glycoprotein ligand-1 recombinant P-selectin glycoprotein ligand-1 PUBTATOR P-selectin glycoprotein ligand-1 100773229 Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin. 3410855 2 19 gly glycosylated 269:280 arg1 chicken ovalbumin chicken ovalbumin PUBTATOR ovalbumin 396058 It has been shown previously that chicken ovalbumin synthesized and secreted in a heterologous cell system is glycosylated at the correct site and that the oligosaccharides at that site, similar to the protein made in hen oviduct, are predominantly of the hybrid type (Sheares, B. T., and Robbins, P. W. (1986) Proc. 26140918 1 53 gly glycoprotein 155:166 arg1 VSTM1-v2 VSTM1-v2 PUBTATOR VSTM1-v2 284415 VSTM1-v2 is a secretory glycoprotein identified by our laboratory. 2479542 1 19 gly glycoprotein 137:148 arg1 E2 E2 Cterm E2 E2 is a 32 kd human T-cell surface glycoprotein involved in spontaneous rosette formation with erythrocytes. 9792503 1 96 gly glycoprotein 148:159 arg1 AAG AAG OGER AAG P29372 Human alpha1-acid glycoprotein (AAG), an acute-phase plasma protein, is heterogeneous in the native state and polymorphic in the desialylated state. 15538777 9 38 gly beta2-glycoprotein 2089:2106 arg1 beta2-glycoprotein I beta2-glycoprotein I OGER beta2-glycoprotein I P02749 The strategy, isolation of glycopeptides followed by MS(n) analysis, efficiently characterized the structures of beta2-glycoprotein I with four N-glycosylation sites and was applied to an analysis of total serum glycoproteins. 7755594 6 80 part_of Asn-25 1018:1023 arg1 IFN-gamma IFN-gamma Asn-25 PUBTATOR SpecificSite IFN-gamma 3458 Asn-25 The glycan residues of IFN-gamma, especially at Asn-25, play an important role in protease resistance. 8349699 7 45 gly N-glycosylation 1047:1061 arg1 murine PGH synthase-2 murine PGH synthase-2 PUBTATOR PGH synthase-2 19225 Using site-directed mutagenesis, we determined that there is an additional site of N-glycosylation in murine PGH synthase-2 located at Asn580. 23236605 4 40 gly contains 724:731 arg1 human AIM AND no N-glycan human AIM no N-glycan OGER AIM P37217 Although human AIM contains no N-glycan, attachment of N-glycans increased AIM secretion. 8243674 1 2 gly antithrombin 164:175 arg1 the carbohydrate-bearing asparagine residues antithrombin the carbohydrate-bearing asparagine residues PUBTATOR antithrombin 462 We have changed one of the carbohydrate-bearing asparagine residues of human antithrombin to glutamine by site-directed mutagenesis and expressed the variant antithrombin, N135Q, in baby hamster kidney cells. 20936810 0 77 gly glycosylation 40:52 arg1 Pin WW folding kinetics Pin WW folding kinetics PUBTATOR Pin 8655 Context-dependent effects of asparagine glycosylation on Pin WW folding kinetics and thermodynamics. 18728239 1 45 gly has 189:191 arg1 B cell-activating factor AND sialylation levels B cell-activating factor multiple O-linked glycosylation sites sialylation levels OGER Site B cell-activating factor receptor 3 Q96RJ3 sites B cell-activating factor receptor 3 (BR3)-Fc is an IgG1-receptor dimeric fusion protein that has multiple O-linked glycosylation sites and sialylation levels that can vary in the manufacturing process. 9654121 1 24 gly N-glycosylated 167:180 arg1 CD80 CD80 PUBTATOR CD80 941 Though the cell surface-associated costimulator B7-1(CD80) is known to be highly N-glycosylated, the functional significance of this N-glycosylation has not been evaluated. 9654121 1 24 gly N-glycosylated 167:180 arg1 the cell surface-associated costimulator B7-1 the cell surface-associated costimulator B7-1 PUBTATOR B7-1 941 Though the cell surface-associated costimulator B7-1(CD80) is known to be highly N-glycosylated, the functional significance of this N-glycosylation has not been evaluated. 16730207 2 6 gly glycosylation 562:574 arg1 IGF-IR IGF-IR PUBTATOR IGF-IR 3480 The pattern of binding to the immobilised lectins indicated that the glycosylation of the IGF-IR, IGF-IIR and IR differed. 24799124 0 43 gly ceruloplasmin 49:61 arg1 Mass-selected site-specific core-fucosylation ceruloplasmin Mass-selected site-specific core-fucosylation PUBTATOR ceruloplasmin 1356 Mass-selected site-specific core-fucosylation of ceruloplasmin in alcohol-related hepatocellular carcinoma. 24799124 0 50 gly core-fucosylation 28:44 arg1 ceruloplasmin ceruloplasmin PUBTATOR ceruloplasmin 1356 Mass-selected site-specific core-fucosylation of ceruloplasmin in alcohol-related hepatocellular carcinoma. 20174636 4 68 part_of env 717:719 arg1 The V1-V5 region env The V1-V5 region PUBTATOR SiteSequence env 100616444 V1-V5 region METHODOLOGY AND FINDINGS: The V1-V5 region of env was amplified from 6 MIPs baseline samples and 334 DNA sequences in total were analyzed. 2340332 3 66 gly attached 649:656 arg2 TeBG AND the oligosaccharides TeBG the oligosaccharides OGER TeBG P04278 The structures of the oligosaccharides attached to TeBG were determined by using serial lectin chromatography. 9427707 8 45 part_of FVIII 1771:1775 arg1 FVIII residues 558 to 565 FVIII FVIII residues 558 to 565 PUBTATOR SpecificSite FVIII 2157 residues 558 To evaluate interaction of FVIII with factor IXa, we performed an inhibition assay using a synthetic peptide corresponding to FVIII residues 558 to 565, previously shown to be a factor IXa interaction site. 10584881 2 19 gly glycoforms 385:394 arg1 AFP AFP PUBTATOR AFP 174 However, there exist glycoforms of AFP which may be more specific for particular tumours. 1371789 8 30 gly R 1475:1475 arg1 complex structure Fc alpha R complex structure PUBTATOR Fc alpha R 2204 These antibodies and native IgA ligands thus provide complementary reagents for definition of the complex structure and function of Fc alpha R in systemic IgA antibody responses. 15252023 9 31 gly contains 1838:1845 arg1 the CI-MPR AND a third Man-6-P recognition site the CI-MPR a third Man-6-P recognition site PUBTATOR CI-MPR 3482 Taken together, these results demonstrate that the CI-MPR contains a third Man-6-P recognition site that is located in domain 5 and that exhibits lower affinity than the carbohydrate-binding sites present in domains 1-3 and 9. 16510764 6 35 gly O-glycosylation 592:606 arg1 IgD IgD OGER IgD P01880 The O-glycosylation profiles of native and desialylated IgA1 and IgD were measured in an ELISA-type system using the lectins Helix aspersa and peanut agglutinin, which bind to alternative forms of O-glycan moieties. 16510764 6 35 gly O-glycosylation 592:606 arg1 IgA1 IgA1 PUBTATOR IgA1 3493 The O-glycosylation profiles of native and desialylated IgA1 and IgD were measured in an ELISA-type system using the lectins Helix aspersa and peanut agglutinin, which bind to alternative forms of O-glycan moieties. 16510764 6 73 gly desialylated 631:642 arg1 IgA1 IgA1 PUBTATOR IgA1 3493 The O-glycosylation profiles of native and desialylated IgA1 and IgD were measured in an ELISA-type system using the lectins Helix aspersa and peanut agglutinin, which bind to alternative forms of O-glycan moieties. 18374598 3 18 gly N-glycosylation 711:725 arg1 the betac-subunit the betac-subunit OGER subunit 3567 Previous studies have reached conflicting conclusions as to whether N-glycosylation of the betac-subunit is necessary for functional GM-CSF, IL-3 and IL-5 receptors. 25759508 0 41 gly O-Glycosylation 13:27 arg1 Human Immunoglobulin G3 Human Immunoglobulin G3 PUBTATOR Human Immunoglobulin G3 3502 Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3). 25759508 0 41 gly O-Glycosylation 13:27 arg1 IgG3 IgG3 PUBTATOR IgG3 3502 Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3). 15313009 0 72 gly heterogeneity 45:57 arg1 human thrombopoietin human thrombopoietin PUBTATOR thrombopoietin 7066 Effect of sodium butyrate on the production, heterogeneity and biological activity of human thrombopoietin by recombinant Chinese hamster ovary cells. 7354085 3 4 gly A 690:690 arg1 the N-glycosidic oligosaccharide glycophorin A the N-glycosidic oligosaccharide PUBTATOR glycophorin A 2993 The synthesis of the N-glycosidic oligosaccharide of glycophorin A is inhibited by the antibiotic tunicamycin, while the O-glycosidic oligosaccharides are not affected. 8286753 2 69 part_of Thr 392:394 arg1 the TfR TfR Thr PUBTATOR SpecificSite TfR 7037 Thr 104 The function of the single O-linked oligosaccharide near the transmembrane domain of the TfR at amino acid Thr 104 is unknown. 12970363 10 21 gly monoglycosylated 1432:1447 arg1 the monoglycosylated TRPC3 the monoglycosylated TRPC3 PUBTATOR TRPC3 7222 Elimination of the e2 glycosylation site, missing in the monoglycosylated TRPC3, was sufficient to convert the tightly receptor-regulated TRPC6 into a constitutively active channel, displaying functional characteristics of TRPC3. 21980282 3 12 gly Env 770:772 arg1 scanning all Env scanning all PUBTATOR Env 100616444 Samples were divided at the outset into hypothesis-forming and validation sets, and we used phylogenetically corrected statistical strategies to identify signatures, systematically scanning all of Env. 25213400 11 26 gly contains 1144:1151 arg1 GP4 AND one sialic acid GP4 one sialic acid PUBTATOR GP4 948 The most mannose rich are the glycans MS2 and GP4, each of them has four mannoses; OPPE1 contains five N-acetylglucosamines and one sulfated glucuronic acid; GP4 contains one sialic acid. 11751598 10 43 gly O-glycosylation 1494:1508 arg1 Sp1 Sp1 OGER Sp1 Q8N907 Glucosamine significantly increased O-glycosylation of Sp1 by 16.1 +/- 4.5% (P = 0.0305; n = 3). 9849653 10 51 gly N-glycosylation 1185:1199 arg1 the EP3alpha receptor the EP3alpha receptor Cterm EP3alpha receptor 19218 These results indicated that N-glycosylation of the EP3alpha receptor could partially affect the affinity and specificity of the ligand binding. 26348848 5 53 gly Kv3.1a 938:943 arg1 N-glycans Kv3 N-glycans PUBTATOR Kv3 29731 Total internal reflection fluorescence microscopy images revealed that N-glycans of Kv3.1a contributed to its placement in the cell membrane while N-glycans had no effect on the distribution of Kv1.1. 8818270 2 19 gly glycoprotein 377:388 arg1 alpha 1-antitrypsin alpha 1-antitrypsin PUBTATOR alpha 1-antitrypsin 5265 The signal sequence derived from inulinase (INU1A) of Kluyveromyces marxianus was evaluated in directing the secretion of a human glycoprotein, alpha 1-antitrypsin (alpha 1-AT), from Saccharomyces cerevisiae. 1316474 0 95 gly glycoproteins 50:62 arg1 gpIV gpIV PUBTATOR gpIV 51206 Receptor properties of two varicella-zoster virus glycoproteins, gpI and gpIV, homologous to herpes simplex virus gE and gI. 1316474 0 95 gly glycoproteins 50:62 arg1 gpI gpI PUBTATOR gpI 2821 Receptor properties of two varicella-zoster virus glycoproteins, gpI and gpIV, homologous to herpes simplex virus gE and gI. 20385559 7 63 gly glycosylation 1289:1301 arg1 ActRIIB ActRIIB PUBTATOR ActRIIB 93 In addition, we show that glycosylation of ActRIIB is not required for binding to activin A or GDF-11. 17158864 11 66 gly glycosylation 1512:1524 arg1 PR3 PR3 PUBTATOR PR3 5657 Finally, a capture ELISA for ANCA detection, using rPR3 glycosylation variants as target antigens, reveals that in about 20% of patients, epitope recognition by ANCA is affected by the glycosylation status of PR3. 2108716 8 39 gly proapoAII 1556:1564 arg1 carbohydrate chains apoAII carbohydrate chains PUBTATOR apoAII 336 The combined data are consistent with the following intra- and/or extracellular modifications of apoAII: (a) modification of the apoAII which results in the net loss of two positive charges; (b) glycosylation of the modified proapoAII with carbohydrate chains containing sialic acid; (c) proteolytic removal of the prosegment and cyclization of the N-terminal glutamine. 2108716 8 77 gly glycosylation 1526:1538 arg1 the modified proapoAII the modified proapoAII PUBTATOR apoAII 336 The combined data are consistent with the following intra- and/or extracellular modifications of apoAII: (a) modification of the apoAII which results in the net loss of two positive charges; (b) glycosylation of the modified proapoAII with carbohydrate chains containing sialic acid; (c) proteolytic removal of the prosegment and cyclization of the N-terminal glutamine. 29273683 11 13 gly glycosylation 1634:1646 arg1 the RCL the RCL OGER RCL O43598 Moreover, while glycosylation of N347 in the RCL limits these activities, N-glycans at other sites also appear to protect CBG from neutrophil elastase or chymotrypsin. 19540231 12 62 gly glycosylated 2208:2219 arg1 glycosylated IGFBP-3 glycosylated IGFBP-3 PUBTATOR IGFBP-3 3486 Strong responses were also observed towards recombinant formulations of non-glycosylated IGFBP-3, glycosylated IGFBP-3 and glycosylated IGFBP-5 (all p < 0.001), while glycosylated IGFBP-4 induced a relatively minor response (p < 0.05). 19540231 12 119 gly glycosylated 2277:2288 arg1 glycosylated IGFBP-4 glycosylated IGFBP-4 PUBTATOR IGFBP-4 3487 Strong responses were also observed towards recombinant formulations of non-glycosylated IGFBP-3, glycosylated IGFBP-3 and glycosylated IGFBP-5 (all p < 0.001), while glycosylated IGFBP-4 induced a relatively minor response (p < 0.05). 19540231 12 123 gly non-glycosylated 2182:2197 arg1 non-glycosylated IGFBP-3 non-glycosylated IGFBP-3 PUBTATOR IGFBP-3 3486 Strong responses were also observed towards recombinant formulations of non-glycosylated IGFBP-3, glycosylated IGFBP-3 and glycosylated IGFBP-5 (all p < 0.001), while glycosylated IGFBP-4 induced a relatively minor response (p < 0.05). 19540231 12 135 gly glycosylated 2233:2244 arg1 glycosylated IGFBP-5 glycosylated IGFBP-5 PUBTATOR IGFBP-5 3488 Strong responses were also observed towards recombinant formulations of non-glycosylated IGFBP-3, glycosylated IGFBP-3 and glycosylated IGFBP-5 (all p < 0.001), while glycosylated IGFBP-4 induced a relatively minor response (p < 0.05). 12663789 2 31 gly glycosylated 265:276 arg1 Both glycosylated and fully deglycosylated CD155 Both glycosylated and fully deglycosylated CD155 PUBTATOR CD155 5817 Both glycosylated and fully deglycosylated CD155 exhibited similar binding sites and orientations in the viral canyon for all three PV serotypes, showing that all three serotypes use a common mechanism for cell entry. 12663789 2 75 gly deglycosylated 288:301 arg1 Both glycosylated and fully deglycosylated CD155 Both glycosylated and fully deglycosylated CD155 PUBTATOR CD155 5817 Both glycosylated and fully deglycosylated CD155 exhibited similar binding sites and orientations in the viral canyon for all three PV serotypes, showing that all three serotypes use a common mechanism for cell entry. 19171054 3 39 gly hyperglycosylated 466:482 arg1 hyperglycosylated hCG hyperglycosylated hCG OGER hCG These are regular hCG, hyperglycosylated hCG and the free beta-subunit of hyperglycosylated hCG. 19171054 3 113 gly hyperglycosylated 415:431 arg1 hyperglycosylated hCG hyperglycosylated hCG OGER hCG These are regular hCG, hyperglycosylated hCG and the free beta-subunit of hyperglycosylated hCG. 17715132 3 18 gly N-glycosylated 480:493 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We show here that Pannexin1 forms a hexameric channel and reaches the cell surface but, unlike connexins, is N-glycosylated. 3360214 9 90 gly glycosylation 1319:1331 arg1 laminin laminin OGER laminin The data for nonenzymatic glycosylation of laminin for 1 day in vitro resulted in a biphasic heparin binding curve. 7904345 0 35 gly Glycosylation 0:12 arg1 CD4 CD4 PUBTATOR CD4 24932 Glycosylation of CD4 and Thy-1. 7904345 0 35 gly Glycosylation 0:12 arg1 Thy-1 Thy-1 PUBTATOR Thy-1 24832 Glycosylation of CD4 and Thy-1. 27048837 1 24 gly glycoprotein 183:194 arg1 Apolipoprotein E Apolipoprotein E PUBTATOR Apolipoprotein E 348 BACKGROUND: Apolipoprotein E (Apo E) is a glycoprotein which acts as a ligand facilitating the uptake of lipids. 9497354 0 74 gly O-glycosylation 83:97 arg1 APP APP Cterm APP 351 Cleavage of Alzheimer's amyloid precursor protein (APP) by secretases occurs after O-glycosylation of APP in the protein secretory pathway. 9792708 1 41 gly contains 144:151 arg1 The mouse hepatitis virus (MHV) membrane (M) protein AND only O-linked oligosaccharides The mouse hepatitis virus (MHV) membrane (M) protein only O-linked oligosaccharides OGER M) protein P54296 The mouse hepatitis virus (MHV) membrane (M) protein contains only O-linked oligosaccharides. 9737988 4 56 gly 3-fucosyltransferase 458:477 arg1 FucT V fucosyltransferase V FucT V OGER fucosyltransferase V Q11128 271, 8818-8823), a domain swapping approach demonstrated that a region of amino acids found in human alpha1, 3/4-fucosyltransferase III (FucT III) conferred a significant increase in alpha1,4-FucT acceptor substrate specificity into alpha1, 3-fucosyltransferase V (FucT V), which, under the same assay conditions, has extremely low alpha1,4-FucT acceptor substrate specificity. 9737988 4 42 gly has 531:533 arg1 3-fucosyltransferase V AND extremely low alpha1,4-FucT acceptor substrate specificity 3-fucosyltransferase V extremely low alpha1,4-FucT acceptor substrate specificity OGER fucosyltransferase V Q11128 271, 8818-8823), a domain swapping approach demonstrated that a region of amino acids found in human alpha1, 3/4-fucosyltransferase III (FucT III) conferred a significant increase in alpha1,4-FucT acceptor substrate specificity into alpha1, 3-fucosyltransferase V (FucT V), which, under the same assay conditions, has extremely low alpha1,4-FucT acceptor substrate specificity. 9429743 1 34 gly glycosylated 122:133 arg1 human serum albumin human serum albumin PUBTATOR serum albumin 213 The binding properties of hypoglycaemic drugs to glycosylated human serum albumin (G-HSA) were investigated using a fluorescence quenching method. 9429743 1 34 gly glycosylated 122:133 arg1 G-HSA G-HSA OGER HSA Q15070 The binding properties of hypoglycaemic drugs to glycosylated human serum albumin (G-HSA) were investigated using a fluorescence quenching method. 17967194 13 51 gly glycosylated 2321:2332 arg1 the secreted mature human r-alpha1-PI the secreted mature human r-alpha1-PI Cterm r-alpha1-PI The heterologous expression of the human gene for alpha1-PI in A. niger was successfully achieved to produce the secreted mature human r-alpha1-PI in A. niger as a biologically active glycosylated protein with improved stability and with yields of up to 12 mg/L in shake-flask growth. 3651384 1 44 gly glycoprotein 102:113 arg1 SGP-2 SGP-2 PUBTATOR 2 (SGP-2 24854 Sulfated glycoprotein 2 (SGP-2) is the major protein secreted by rat Sertoli cells. 3651384 1 44 gly glycoprotein 102:113 arg1 Sulfated glycoprotein 2 Sulfated glycoprotein 2 PUBTATOR Sulfated glycoprotein 2 24854 Sulfated glycoprotein 2 (SGP-2) is the major protein secreted by rat Sertoli cells. 29119347 6 3 gly gm1-IDUA 1919:1926 arg1 the high-mannose N-glycans gm1-IDUA the high-mannose N-glycans PUBTATOR gm1-IDUA 3425 This may be due to the greater number of mannose residues comprising the high-mannose N-glycans of gm1-IDUA. 22849435 10 33 gly glycosylation 1565:1577 arg1 vWF function vWF function PUBTATOR vWF 7450 The delineation of such varied glycan populations in conjunction with current models explaining vWF activity will facilitate research aimed at providing a better understanding of the influence of glycosylation on vWF function. 8098269 4 36 gly P-glycoprotein 455:468 arg1 P-glycoprotein P-glycoprotein PUBTATOR METHODS: P-glycoprotein 5243 METHODS: P-glycoprotein was detected using immunoblotting with a monoclonal antibody against it, C219. 24291635 8 51 gly Hypo-glycosylated 1103:1119 arg1 Hypo-glycosylated hFSH Hypo-glycosylated hFSH OGER hFSH Hypo-glycosylated hFSH(21/18) also bound rat FSHRs more rapidly, exhibiting almost no lag in binding, whereas hFSH(24) specific binding proceeded very slowly for almost the first hour of incubation. 15592895 1 5 gly glycoprotein 153:164 arg1 The dengue 2 virus (DENV-2) NS1 glycoprotein The dengue 2 virus (DENV-2) NS1 glycoprotein PUBTATOR NS1 glycoprotein 10625 The dengue 2 virus (DENV-2) NS1 glycoprotein contains two potential sites for N-linked glycosylation at Asn-130 and Asn-207. 18952059 3 36 gly unglycosylated 302:315 arg1 unglycosylated IZUMO unglycosylated IZUMO PUBTATOR IZUMO 73456 In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. 18952059 3 36 gly unglycosylated 302:315 arg1 N204Q-IZUMO N204Q-IZUMO PUBTATOR -IZUMO 73456 In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q-IZUMO) in Izumo1 -/- background. 2456913 1 55 gly glycosylation 250:262 arg1 mouse TSH mouse TSH OGER TSH We have determined the structures of high mannose (Man) oligosaccharide units at individual glycosylation sites of mouse TSH. 1367433 6 16 part_of t-PA 1238:1241 arg1 asn 117 t-PA asn 117 PUBTATOR SpecificSite t-PA 18791 asn 117 We found that with one exception, all mutant activators lack the high mannose glycan found at asn 117 of native t-PA. 16227249 9 72 gly glycosylated 1116:1127 arg1 prM prM Cterm prM RVPs or virions bearing combinations of glycosylated and nonglycosylated forms of prM and E could infect mammalian, avian, and mosquito cells (BHK-21, QT6, and C6/36, respectively). 16227249 9 75 gly nonglycosylated 1133:1147 arg1 prM prM Cterm prM RVPs or virions bearing combinations of glycosylated and nonglycosylated forms of prM and E could infect mammalian, avian, and mosquito cells (BHK-21, QT6, and C6/36, respectively). 9597755 1 51 gly glycoprotein 152:163 arg1 Thrombomodulin Thrombomodulin PUBTATOR Thrombomodulin 7056 Thrombomodulin (TM) is an anticoagulant glycoprotein on the surface of endothelial cell that directly inhibits the procoagulant activities of thrombin, and the TM-thrombin complex accelerates thrombin-catalyzed activation of protein C. Soluble TM in urine has no glycosaminoglycan (GAG) chain which accelerates the anticoagulant activities. 8643693 7 23 gly underglycosylated 1359:1375 arg1 underglycosylated mucin underglycosylated mucin PUBTATOR mucin 100508689 Already after the first injection we found in the peripheral blood measurable frequency of cytotoxic T-cell precursors specific for underglycosylated mucin. 11063734 11 59 gly SP-B 1581:1584 arg1 a determinant SP-B a determinant PUBTATOR SP-B 6439 We propose that the SP-B Ile131Thr polymorphism is a determinant for certain SP-A alleles as factors causing genetic susceptibility to RDS (6A(2), 1A(0)) or protection against it (6A(3), 1A(2)). 24806200 0 62 gly glycoprotein 96:107 arg1 glycoprotein hormones glycoprotein hormones PUBTATOR chorionic gonadotropin (hCG) glycoprotein 93659 Chemical synthesis of the β-subunit of human luteinizing (hLH) and chorionic gonadotropin (hCG) glycoprotein hormones. 14978164 7 50 gly glycosylated 893:904 arg1 aberrantly glycosylated IgA1 aberrantly glycosylated IgA1 PUBTATOR IgA1 3493 Serum IgA immune complexes from patients with IgAN containing aberrantly glycosylated IgA1 bound more avidly to TfR than those from normal individuals. 12724313 6 77 gly OIP106 718:723 arg1 the O-GlcNAcylation OIP106 the O-GlcNAcylation PUBTATOR OIP106 22906 Here, we further investigated the role of the TPR domain in the O-GlcNAcylation of OIP106, one of the members of this OIP family. 28958711 1 7 gly glycosylated 85:96 arg1 BSP BSP PUBTATOR BSP 3381 The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. 28958711 1 7 gly glycosylated 85:96 arg1 The highly glycosylated bone sialoprotein The highly glycosylated bone sialoprotein PUBTATOR bone sialoprotein 3381 The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. 17534424 3 19 gly N-glycosylated 766:779 arg1 human ClC-6 human ClC-6 PUBTATOR ClC-6 1185 PRINCIPAL FINDINGS: Using a polyclonal affinity-purified antibody directed against a unique epitope in the ClC-6 COOH-terminal tail, we show that human ClC-6, when transfected in COS-1 cells, is N-glycosylated in a region that is evolutionary poorly conserved between mammalian CLC proteins and that is located between the predicted helices K and M. Three asparagine residues (N410, N422 and N432) have been defined by mutagenesis as acceptor sites for N-glycosylation, but only two of the three sites seem to be simultaneously N-glycosylated. 26850929 1 6 gly glycosylated 191:202 arg1 HirudinP6 HirudinP6 Cterm P6 HirudinP6 is a glycosylated and sulfated high affinity thrombin inhibitory protein isolated from Hirudineria manillensis. 26618514 8 66 gly sialylated 1107:1116 arg1 the sialylated anti-SNEC IgG the sialylated anti-SNEC IgG Cterm anti-SNEC Ig Moreover, the sialylated anti-SNEC IgG was not simply anti-inflammatory, but switched the cytokine secretion profiles from interleukin (IL)-6/IL-8 to tumour necrosis factor (TNF)-α/IL-1β. 1845821 1 66 gly glycoprotein 316:327 arg1 glycoprotein D glycoprotein D OGER glycoprotein D Q16570 DNA sequence analysis of one-third of the unique short (Us) segment of the equine herpesvirus type 1 (EHV-1) genome revealed an open reading frame (ORF) whose translated sequence exhibits significant homology to glycoprotein D of herpes simplex virus (HSV) types 1 and 2 and to pseudorabies virus (PRV) glycoprotein 50, the gD equivalent. 21541302 2 58 gly attached 220:227 arg2 the Kv3.1 glycoprotein AND N-glycans the Kv3.1 glycoprotein N-glycans PUBTATOR Kv3.1 glycoprotein 3746 The role of N-glycans attached to the Kv3.1 glycoprotein on conducting and non-conducting functions of the Kv3.1 channel are quite limiting. 21541302 2 60 gly glycoprotein 242:253 arg1 the Kv3.1 glycoprotein the Kv3.1 glycoprotein PUBTATOR Kv3.1 glycoprotein 3746 The role of N-glycans attached to the Kv3.1 glycoprotein on conducting and non-conducting functions of the Kv3.1 channel are quite limiting. 2394722 1 10 gly deglycosylated 107:120 arg1 deglycosylated human pancreatic tumor mucin deglycosylated human pancreatic tumor mucin PUBTATOR mucin 100508689 A monospecific polyclonal antiserum against deglycosylated human pancreatic tumor mucin was used to select human pancreatic mucin cDNA clones from a lambda gt11 cDNA expression library developed from a human pancreatic tumor cell line. 2050549 2 37 gly glycosylation 460:472 arg1 BSA BSA Cterm BSA 11657 The carbohydrate-binding sites were localized with FITC-coupled neoglycoproteins, synthesized by chemical glycosylation of bovine serum albumin (BSA). 2050549 2 37 gly glycosylation 460:472 arg1 bovine serum albumin bovine serum albumin PUBTATOR serum albumin 11657 The carbohydrate-binding sites were localized with FITC-coupled neoglycoproteins, synthesized by chemical glycosylation of bovine serum albumin (BSA). 9831647 0 40 gly glycoprotein 18:29 arg1 DAN DAN PUBTATOR DAN 108697874 DAN is a secreted glycoprotein related to Xenopus cerberus. 16959765 1 11 gly N-glycosylation 190:204 arg1 integrin alpha5beta1 integrin alpha5beta1 Cterm alpha5beta1 The N-glycosylation of integrin alpha5beta1 is thought to play crucial roles in cell spreading, cell migration, ligand binding, and dimer formation, but the underlying mechanism remains unclear. 10374967 0 10 gly glycoprotein 58:69 arg1 glycoprotein H glycoprotein H Cterm herpesvirus-6 glycoprotein H N- and C-terminal external domains of human herpesvirus-6 glycoprotein H affect a fusion-associated conformation mediated by glycoprotein L binding the N terminus. 10374967 0 45 gly glycoprotein 125:136 arg1 glycoprotein L glycoprotein L Cterm glycoprotein L N- and C-terminal external domains of human herpesvirus-6 glycoprotein H affect a fusion-associated conformation mediated by glycoprotein L binding the N terminus. 8631363 9 62 gly 0-glycosylation 1551:1565 arg1 tumor necrosis factor-alpha tumor necrosis factor-alpha PUBTATOR tumor necrosis factor-alpha 7124 An amino acid sequence analysis of the glycosylated peptides was performed after Staphylococcus aureus V8 protease digestion of tumor necrosis factor-alpha had been completed, and it was proved that the 0-glycosylation site of tumor necrosis factor-alpha was Ser 4. 15322230 4 13 gly P-glycoprotein 569:582 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Transfection of multidrug-resistant cells with wild-type ubiquitin or treatment with an N-glycosylation inhibitor increased the ubiquitination of P-glycoprotein and increased P-glycoprotein degradation. 15322230 4 61 gly P-glycoprotein 598:611 arg1 increased P-glycoprotein degradation increased P-glycoprotein degradation PUBTATOR P-glycoprotein 5243 Transfection of multidrug-resistant cells with wild-type ubiquitin or treatment with an N-glycosylation inhibitor increased the ubiquitination of P-glycoprotein and increased P-glycoprotein degradation. 9365923 1 34 gly glycoprotein 157:168 arg1 Human alpha-lactalbumin Human alpha-lactalbumin PUBTATOR Human alpha-lactalbumin 3906 Human alpha-lactalbumin has not been described as a glycoprotein, despite the fact that several alpha-lactalbumins of both ruminant and nonruminant species are known to be glycosylated. 11559807 0 76 gly determinants 10:21 arg1 the coronavirus receptor aminopeptidase N aminopeptidase N determinants PUBTATOR aminopeptidase N 290 Molecular determinants of species specificity in the coronavirus receptor aminopeptidase N (CD13): influence of N-linked glycosylation. 11559807 0 76 gly determinants 10:21 arg1 CD13 CD13 determinants PUBTATOR CD13 290 Molecular determinants of species specificity in the coronavirus receptor aminopeptidase N (CD13): influence of N-linked glycosylation. 27734143 7 47 gly glycoprotein 1065:1076 arg1 AGP AGP Cterm AGP We show this using α-1-acid glycoprotein (AGP) mixed into a set of increasingly complex matrices. 27734143 7 47 gly glycoprotein 1065:1076 arg1 this using α-1-acid glycoprotein this using α-1-acid glycoprotein Cterm α-1-acid We show this using α-1-acid glycoprotein (AGP) mixed into a set of increasingly complex matrices. 8621728 1 26 gly glycoprotein 135:146 arg1 P-selectin glycoprotein ligand-1 P-selectin glycoprotein ligand-1 PUBTATOR P-selectin glycoprotein ligand-1 6404 P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like ligand for P- and E-selectin on human leukocytes. 9524075 9 36 gly non-glycosylated 1048:1063 arg1 the glycosylated and non-glycosylated procathepsin S the glycosylated and non-glycosylated procathepsin S Cterm the glycosylated and non-glycosylated procathepsin S 1520 A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. 9524075 9 55 gly glycosylated 1031:1042 arg1 the glycosylated and non-glycosylated procathepsin S the glycosylated and non-glycosylated procathepsin S Cterm the glycosylated and non-glycosylated procathepsin S 1520 A reuptake of the glycosylated and non-glycosylated procathepsin S by HEK 293-cells could be observed. 16014566 1 11 gly glycoprotein 109:120 arg1 human glycoprotein VI (GPVI) human glycoprotein VI (GPVI) PUBTATOR glycoprotein VI 51206 Using recombinant human glycoprotein VI (GPVI), we evaluated the effect of N-linked glycosylation at the consensus site Asparagine92-Glycine-Serine94 (N92GS94) on binding of this platelet-specific receptor to its ligands, human type I collagen, collagen-related peptide (CRP), and the snake venom C-type lectin convulxin (CVX). 2673431 6 4 gly has 809:811 arg1 Circulating vWF AND a complex multimeric structure Circulating vWF a complex multimeric structure PUBTATOR Circulating vWF 7450 Circulating vWF has a complex multimeric structure that ranges in Mrs from 0.5 to 20 x 10(6) Daltons. 10413524 10 31 gly moieties 1190:1197 arg1 mouse FcRn FcRn moieties PUBTATOR FcRn 14132 Using a gel-filtration assay under nonequilibrium conditions, we find that both forms of FcRn produce 2:1 receptor-ligand complexes, but that alterations of the carbohydrate moieties on mouse FcRn can result in an apparent stoichiometry of 1:1. 27604319 5 62 gly glycosylation 949:961 arg1 gp120 gp120 PUBTATOR gp120 3700 While most prior studies have focused on glycan analysis of recombinant forms of gp120, here we report the first systematic glycosylation site analysis of gp120 derived from virions produced by infected T lymphoid cells and show that a single site is exclusively substituted with complex glycans. 8522591 7 48 gly glycosylation 1101:1113 arg1 detergent solubilized K8/18 detergent solubilized K8/18 PUBTATOR K8/18 16691 A relative increase in the phosphorylation and glycosylation of detergent solubilized K8/18 was also noted in vitro and in transgenic animals that express mutant K18. 8651931 9 11 gly glycosylation 1130:1142 arg1 mouse DPP IV mouse DPP IV PUBTATOR DPP IV 13482 Our results suggest that conserved residue Asp599 is important for the proper folding, glycosylation and transport of mouse DPP IV. 8743131 0 2 gly 2-glycoprotein 36:49 arg1 beta 2-glycoprotein I beta 2-glycoprotein I OGER beta 2-glycoprotein I P02749 Anticardiolipin antibodies and beta 2-glycoprotein I. 2001369 3 58 gly rCD4 662:665 arg1 the Asn-linked oligosaccharides rCD4 the Asn-linked oligosaccharides PUBTATOR rCD4 24932 In the present study, the structures of the Asn-linked oligosaccharides of soluble rCD4 have been elucidated. 2462021 0 29 gly glycoprotein 37:48 arg1 myelin-associated glycoprotein myelin-associated glycoprotein PUBTATOR myelin-associated glycoprotein 4099 Phosphorylation of myelin-associated glycoprotein in vivo and in vitro occurs only in the cytoplasmic domain of the large isoform. 17599380 3 20 part_of HIV 326:328 arg1 Asp 386 HIV Env variant Asp 386 PUBTATOR SpecificSite HIV Env variant 100616444 Asp 386 Here, we identify an HIV Env variant in the V4 region of gp120, Asp 386 (D386), that eliminates an N-linked glycosylation site at position 386, enhances viral replication in macrophages, and is present at a higher frequency in AIDS patients with HIV-associated dementia (HAD) compared with non-HAD patients. 17599380 3 70 part_of Env 330:332 arg1 Asp 386 HIV Env variant Asp 386 PUBTATOR SpecificSite HIV Env variant 100616444 Asp 386 Here, we identify an HIV Env variant in the V4 region of gp120, Asp 386 (D386), that eliminates an N-linked glycosylation site at position 386, enhances viral replication in macrophages, and is present at a higher frequency in AIDS patients with HIV-associated dementia (HAD) compared with non-HAD patients. 10971587 2 87 gly glycosylated 313:324 arg1 The ANP receptor The ANP receptor OGER ANP receptor P01161 The ANP receptor that mediates these actions is a glycosylated transmembrane protein coupled to guanylate cyclase. 18982490 6 3 gly glycoprotein 1048:1059 arg1 The expressed UDPGT The expressed UDPGT PUBTATOR UDPGT 54657 The expressed UDPGT was a glycoprotein as indicated by electrophoretic mobility shift in Mr approximately 3,000-4,000 when expressed in the presence of tunicamycin. 23050552 8 46 gly fibrinogen 1086:1095 arg1 The previously reported N-glycan attachment sites fibrinogen The previously reported N-glycan attachment sites PUBTATOR fibrinogen 2244 The previously reported N-glycan attachment sites of human fibrinogen could be confirmed. 7925397 3 6 gly form 721:724 arg1 the tandem-repeat array form of MUC1 the tandem-repeat array PUBTATOR form of MUC1 4582 The nucleotide sequence of this novel MUC1 mRNA demonstrates that it is identical to the MUC1 cDNA sequences downstream and upstream to the tandem-repeat array of the transmembrane form of MUC1. 1991473 6 15 gly attached 776:783 arg1 hLH alpha AND The oligosaccharides hLH alpha The oligosaccharides PUBTATOR hLH alpha 1081 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 1991473 6 15 gly attached 776:783 arg1 hLH beta AND The oligosaccharides hLH beta The oligosaccharides PUBTATOR hLH beta 3972 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 29153507 2 7 gly glycosylated 374:385 arg1 glycosylated human afamin glycosylated human afamin PUBTATOR afamin 173 The 2.1-Å crystal structure of glycosylated human afamin reveals an almost exclusively hydrophobic binding cleft capable of harboring large hydrophobic moieties. 11858723 3 53 gly glycosylated 463:474 arg1 The glycosylated FAP The glycosylated FAP PUBTATOR FAP 2191 The glycosylated FAP was purified to near homogeneity using immobilized metal affinity chromatography and was shown to have both postprolyl dipeptidyl peptidase and postgelatinase activities. 1457969 2 72 gly glycosylated 414:425 arg1 hTSH beta hTSH beta PUBTATOR hTSH beta 7252 Highly purified, biologically active human thyrotrophin (hTSH) was dissociated into its subunits hTSH alpha (glycosylated at Asn 52 and Asn 78) and hTSH beta (glycosylated at Asn 23). 1457969 2 85 gly glycosylated 364:375 arg1 hTSH alpha hTSH alpha PUBTATOR hTSH alpha 1081 Highly purified, biologically active human thyrotrophin (hTSH) was dissociated into its subunits hTSH alpha (glycosylated at Asn 52 and Asn 78) and hTSH beta (glycosylated at Asn 23). 9030779 2 63 gly sphingomyelinase 379:394 arg1 the six potential N-linked oligosaccharide chains acid sphingomyelinase the six potential N-linked oligosaccharide chains PUBTATOR acid sphingomyelinase 6609 We have determined the influence of the six potential N-linked oligosaccharide chains of human acid sphingomyelinase (ASM) on catalytic activity, targeting, and processing of the enzyme. 17960575 5 2 gly FETUA 681:685 arg1 all three N-glycosylation sites FETUA all three N-glycosylation sites PUBTATOR FETUA 197 Glycopeptides derived from all three N-glycosylation sites of FETUA were observed, and the corresponding CID spectra proved the respective glycans to be oligosaccharides of the triantennary complex type. 17960575 5 36 gly N-glycosylation 656:670 arg1 FETUA FETUA PUBTATOR FETUA 197 Glycopeptides derived from all three N-glycosylation sites of FETUA were observed, and the corresponding CID spectra proved the respective glycans to be oligosaccharides of the triantennary complex type. 10652209 1 13 gly asialoglycoprotein 167:184 arg1 rat asialoglycoprotein receptor rat asialoglycoprotein receptor PUBTATOR hepatic lectin-1 (RHL-1) subunit of rat asialoglycoprotein receptor 24210 We have previously reported that the rat hepatic lectin-1 (RHL-1) subunit of rat asialoglycoprotein receptor (ASGPr), the endocytic receptor found on the basolateral surface of hepatocytes, was expressed in rat thyroid tissue and localized on the apical surface of polarized rat thyroid FRT cells. 8898911 4 26 gly hCG 622:624 arg1 the N-glycans hCG the N-glycans PUBTATOR hCG 93659 In this study, an almost complete 1H-NMR and a partial 13C-NMR spectral assignment for the amino acids and the N-glycans of alpha hCG and of an enzymatically deglycosylated form, which had a single GlcNAc residue at each of its two glycosylation sites, has been achieved. 23776650 8 2 gly glycosylation 1037:1049 arg1 the DCIR the DCIR PUBTATOR DCIR 50856 Nevertheless, altering the glycosylation status of the DCIR expressing cell or mutating the N-glycosylation site of DCIR itself did not increase glycan binding. 23776650 8 41 gly N-glycosylation 1102:1116 arg1 DCIR itself DCIR itself PUBTATOR DCIR 50856 Nevertheless, altering the glycosylation status of the DCIR expressing cell or mutating the N-glycosylation site of DCIR itself did not increase glycan binding. 26641950 3 39 gly glycosylation 711:723 arg1 recombinant human interferon-β1 recombinant human interferon-β1 PUBTATOR interferon-β1 3456 Here, we report on capillary zone electrophoresis (CZE) coupled via a commercial CESI sheathless interface to an Orbitrap ELITE MS for the intact analysis of recombinant human interferon-β1 (Avonex, rhIFN-β1), a biopharmaceutical with complex glycosylation at a single N-linked site. 28668641 1 34 gly glycoprotein 128:139 arg1 Vaspin Vaspin PUBTATOR Vaspin 145264 Vaspin is a glycoprotein with three predicted glycosylation sites at asparagine residues located in proximity to the reactive center loop and close to domains that play important roles in conformational changes underlying serpin function. 18707900 0 10 gly N-glycosylation 51:65 arg1 recombinant IgGs recombinant IgGs Cterm IgGs Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs. 20079467 4 0 gly GcMAF 661:665 arg1 only a single GalNAc GcMAF only a single GalNAc PUBTATOR GcMAF 2638 It has been suggested that Gc globulin subjected sequentially to sialidase and galactosidase treatment generates GcMAF in the form of Gc globulin with only a single GalNAc attached to T420. 25855029 3 75 gly clusterin 492:500 arg1 the N-glycan profile clusterin the N-glycan profile PUBTATOR clusterin 1191 We recently reported that the N-glycan profile of clusterin is altered in the plasma of ccRCC patients. 8496193 8 30 gly glycosylated 1239:1250 arg1 glycosylated procathepsin L glycosylated procathepsin L OGER procathepsin L P06797 Nonglycosylated and endogenous glycosylated procathepsin L were secreted by NIH 3T3 cells with identical kinetics. 21931781 4 24 gly N-glycosylation 604:618 arg1 PrP PrP PUBTATOR PrP( 5621 In this study we have mutated the first, the second, and both N-glycosylation sites of PrP(C) and also replaced the GPI-anchor of PrP(C) by the Thy-1 GPI-anchor in order to investigate the role of these signals in sorting of PrP(C) in MDCK cells. 23389049 7 18 gly haptoglobin 1121:1131 arg1 multiply fucosylated Lewis Y glycoforms haptoglobin multiply fucosylated Lewis Y glycoforms PUBTATOR haptoglobin 3240 The presence of multiply fucosylated Lewis Y glycoforms of haptoglobin in the disease context could have important functional implications. 23389049 7 35 gly fucosylated 1087:1097 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 The presence of multiply fucosylated Lewis Y glycoforms of haptoglobin in the disease context could have important functional implications. 23389049 7 57 gly glycoforms 1107:1116 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 The presence of multiply fucosylated Lewis Y glycoforms of haptoglobin in the disease context could have important functional implications. 18695951 0 40 gly glycosylation 48:60 arg1 recombinant human coagulation factor VLLa recombinant human coagulation factor VLLa Cterm factor VLLa Analysis of the site-specific asparagine-linked glycosylation of recombinant human coagulation factor VLLa by glycosidase digestions, liquid chromatography, and mass spectrometry. 8360173 5 13 gly possess 930:936 arg1 HMW-AR2 AND complex or hybrid type N-linked oligosaccharide structures HMW-AR2 complex or hybrid type N-linked oligosaccharide structures PUBTATOR HMW 57587 HMW-AR1 and HMW-AR2 were found to possess complex or hybrid type N-linked oligosaccharide structures that contained sialic acid. 8360173 5 13 gly possess 930:936 arg1 HMW-AR1 AND complex or hybrid type N-linked oligosaccharide structures HMW-AR1 complex or hybrid type N-linked oligosaccharide structures PUBTATOR AR1 6942 HMW-AR1 and HMW-AR2 were found to possess complex or hybrid type N-linked oligosaccharide structures that contained sialic acid. 23269669 6 16 gly glycosylation 1148:1160 arg1 GC-C GC-C PUBTATOR GC-C 2984 Systematic mutagenesis of each of the 10 sites of glycosylation in GC-C, either singly or in combination, identified two sites that were critical for ligand binding and two that regulated ST-mediated activation. 9973491 1 1 gly glycoprotein 132:143 arg1 IgG IgG Cterm IgG IgG is a glycoprotein with an N-linked carbohydrate structure attached to the CH2 domain of each of its heavy chains. 8193553 8 29 gly had 1392:1394 arg1 Recombinant glycophorin A AND the same O-glycan composition Recombinant glycophorin A the same O-glycan composition PUBTATOR Recombinant glycophorin A 2993 Recombinant glycophorin A had the same O-glycan composition, whether purified from clones expressing high or moderate levels of the recombinant glycoprotein. 30058762 4 68 gly N-glycosylation 795:809 arg1 the IDUA the IDUA OGER IDUA P35475 Indeed, on each of the 6 N-glycosylation sites of the IDUA, a single N-glycan composed of a core Man3 GlcNAc2 carrying one beta(1,2)-xylose and one alpha(1,3)-fucose epitope (M3XFGN2) was identified, highlighting the high homogeneity of the production system. 11067927 5 3 gly glycosylation 827:839 arg1 QPP enzymatic activity QPP enzymatic activity PUBTATOR QPP 29952 Interestingly, this glycosylation is required for QPP enzymatic activity, but not for its localization. 20592872 8 4 part_of receptor 828:835 arg1 the N53 site mu-opioid receptor the N53 site OGER SpecificSite mu-opioid receptor P33535 N53 site Therefore, based on these results, it seems that glycosylation at the N53 site of the rat mu-opioid receptor does not influence the function of this receptor significantly. 15498570 0 24 gly glycoprotein 78:89 arg1 hPAP21 hPAP21 PUBTATOR hPAP21 84279 N-glycosylation is required for efficient secretion of a novel human secreted glycoprotein, hPAP21. 15498570 0 41 gly N-glycosylation 0:14 arg1 hPAP21 hPAP21 PUBTATOR hPAP21 84279 N-glycosylation is required for efficient secretion of a novel human secreted glycoprotein, hPAP21. 12873131 6 61 gly antithrombin 1280:1291 arg1 an intermediate pentasaccharide-bound conformation antithrombin an intermediate pentasaccharide-bound conformation PUBTATOR antithrombin 462 Here we present the structure of an intermediate pentasaccharide-bound conformation of antithrombin which has undergone all of the conformational changes associated with activation except loop expulsion and helix D elongation. 18823996 0 55 gly glycoprotein 43:54 arg1 Orosomucoid Orosomucoid Cterm Orosomucoid The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. 7492686 13 0 gly glycoprotein 1922:1933 arg1 human oviduct-specific glycoprotein human oviduct-specific glycoprotein PUBTATOR oviduct-specific glycoprotein 5016 The HOGP showed high amino acid sequence homology with baboon, bovine, and human oviduct-specific glycoprotein. 14500524 9 39 gly presence 1529:1536 arg1 the T. gondii recombinant gp40 AND terminal O-linked alpha-GalNAc determinants the T. gondii recombinant gp40 terminal O-linked alpha-GalNAc determinants OGER gp40 P09564 The presence of terminal O-linked alpha-GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha-GalNAc residues, and digestion with alpha-N-acetylgalactosaminidase. 16834341 6 27 part_of hFSH 872:875 arg1 Asn7 hFSH Asn7 OGER AminoAcid hFSH Asn7 For instance, except for one site in the beta subunit (Asn7) of hFSH all other sites in both species have sulfated glycoforms. 18215327 1 33 gly glycoprotein 260:271 arg1 the Human Immunodeficiency Virus (HIV) envelope glycoprotein the Human Immunodeficiency Virus (HIV) envelope glycoprotein PUBTATOR HIV) envelope glycoprotein 100616444 BACKGROUND: N-linked glycosylation is a major mechanism for minimizing virus neutralizing antibody response and is present on the Human Immunodeficiency Virus (HIV) envelope glycoprotein. 7821750 0 6 gly interactions 24:35 arg1 IgG IgG interactions Cterm IgG Oligosaccharide-protein interactions in IgG can modulate recognition by Fc gamma receptors. 18940796 1 3 gly dystrophin-glycoprotein 89:111 arg1 The dystrophin-glycoprotein complex The dystrophin-glycoprotein complex OGER dystrophin P11531 The dystrophin-glycoprotein complex and the alpha7beta1 integrin are trans-sarcolemmal linkage systems that connect and transduce contractile forces between muscle fibers and the extracellular matrix. 8400241 10 22 gly containing 1906:1915 arg1 Mi.I mutant glycophorin A AND truncated O-linked oligosaccharides Mi.I mutant glycophorin A truncated O-linked oligosaccharides PUBTATOR glycophorin A 2993 When the transfected IdlD cells were cultured in the presence of N-acetylgalactosamine alone, only intermediate levels of cell surface expression were seen for Mi.I mutant glycophorin A containing truncated O-linked oligosaccharides. 8380735 1 9 gly glycoproteins 171:183 arg1 Hepatocyte Growth Factor Hepatocyte Growth Factor PUBTATOR Hepatocyte Growth Factor (HGF) 3082 Hepatocyte Growth Factor (HGF) and Scatter Factor (SF) are identical glycoproteins secreted by cells of mesodermal origin. 21712391 5 60 gly sites 770:774 arg1 human GGT GGT sites OGER GGT Using site-directed mutagenesis, we confirmed that all seven N-glycosylation sites on human GGT are modified by N-glycans. 7510249 4 71 gly glycosylated 733:744 arg1 K8/18 K8/18 PUBTATOR K8/18 3856 K8/18 expressed in Sf9 cells were glycosylated (O-linked N-acetylglucosamine) and phosphorylated, and each modification occurred on different molecules of K8 and K18, as previously found in human HT29 cells. 9111139 0 60 gly beta-subunit 82:93 arg1 N-linked oligosaccharide structures chorionic gonadotropin beta-subunit N-linked oligosaccharide structures PUBTATOR chorionic gonadotropin beta-subunit 1082 Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin beta-subunit by disruption of disulfide bonds. 21235484 1 48 gly E 264:264 arg1 medically important structural properties protein E medically important structural properties Cterm protein E The aim of this study was prediction of epitopes and medically important structural properties of protein E of Alkhurma hemorrhagic fever virus (AHFV) and comparing these features with two closely relates viruses, i.e. Kyasanur Forest disease virus (KFDV) and Tick-borne encephalitis virus (TBEV) by bioinformatics tools. 29769321 7 60 part_of RpNCRD 1390:1395 arg1 Asn-303 RpNCRD Asn-303 Cterm SpecificSite RpNCRD 397198 Asn-303 MS analysis revealed an N-glycan site-occupancy of >98% at Asn-303 of RpNCRD with complex-type, heterogeneously branched and predominantly α(2,3)-sialylated oligosaccharides. 22511785 1 46 gly glycosylated 276:287 arg1 Signal regulatory protein α Signal regulatory protein α PUBTATOR Signal regulatory protein α 140885 Signal regulatory protein α (SIRPα), a highly glycosylated type-1 transmembrane protein, is composed of three immunoglobulin-like extracellular loops as well as a cytoplasmic tail containing three classical tyrosine-based inhibitory motifs. 17008541 1 59 gly glycoprotein 131:142 arg1 GP GP Cterm GP It is widely accepted that glycoprotein (GP) Ib contains one Ibalpha and one Ibbeta subunit that are connected by a disulfide bond. 2747653 6 13 gly glycosylated 1107:1118 arg1 The 74K albumin The 74K albumin PUBTATOR 74K albumin 24186 The 74K albumin (unlike the 68K albumin) is glycosylated; a point mutation converting Lys256 to Asn introduces an N-linked glycosylation site that is similar to one found in the sequence of mammalian alpha-fetoproteins. 18682497 5 75 gly Unglycosylated 726:739 arg1 Unglycosylated CFTR Unglycosylated CFTR PUBTATOR Unglycosylated CFTR 1080 Unglycosylated CFTR, generated by removal of glycosylation sites or treatment of cells with the N-glycosylation inhibitor tunicamycin, did not bind calnexin, but did traffic to the cell surface and exhibited chloride channel activity. 29071407 2 54 gly glycoprotein 193:204 arg1 GP GP Cterm GP Less appreciated is the human glycoprotein (GP) repertoire (proteoglycome!) 9654121 2 60 gly N-glycosylation 325:339 arg1 human B7-1 function human B7-1 function PUBTATOR B7-1 941 Two experimental approaches were taken to assess the influence of N-glycosylation on human B7-1 function. 15576633 2 56 gly oligosaccharides 374:389 arg1 hOAT4 hOAT4 oligosaccharides PUBTATOR hOAT4 55867 Inhibition of acquisition of oligosaccharides in hOAT4 by mutating asparagine to glutamine and by tunicamycin treatment was combined with the expression of wild-type hOAT4 in a series of mutant Chinese hamster ovary (CHO)-Lec cells defective in the different steps of glycosylation processing. 17496250 11 1 gly O-glycosylated 1382:1395 arg1 IGFBP-5 IGFBP-5 PUBTATOR IGFBP-5 3488 IGFBP-5 was heterogeneously O-glycosylated mainly by sialylated core 1 type glycans. 25187573 2 84 gly glycosylation 355:367 arg1 lubricin lubricin PUBTATOR lubricin 10216 Thus, a site-specific investigation of the glycosylation of lubricin was undertaken, in order to further understand the pathological mechanisms involved in these diseases. 6284780 8 60 gly ACTH 1373:1376 arg1 all three diseases ACTH all three diseases PUBTATOR ACTH 5443 This suggests that the excess ACTH in all three diseases arises from the same precursor POMC molecule. 8099782 2 31 gly B 360:360 arg1 The carbohydrate chain saposin B The carbohydrate chain Cterm saposin B The carbohydrate chain of saposin B was removed and deglycosylated saposin B was characterized and compared with native saposin B. Deglycosylated saposin B stimulated the enzymatic hydrolysis of ganglioside GM1 by acid beta-galactosidase and sulfatide by arylsulfatase A to the same extent as native saposin B. 8099782 2 73 gly deglycosylated 378:391 arg1 deglycosylated saposin B deglycosylated saposin B Cterm deglycosylated saposin B The carbohydrate chain of saposin B was removed and deglycosylated saposin B was characterized and compared with native saposin B. Deglycosylated saposin B stimulated the enzymatic hydrolysis of ganglioside GM1 by acid beta-galactosidase and sulfatide by arylsulfatase A to the same extent as native saposin B. 8051068 7 9 part_of PGHS-1 1596:1601 arg1 Arg277 PGHS-1 Arg277 PUBTATOR AminoAcid PGHS-1 19224 Arg277 Accordingly, our results indicate that the trypsin cleavage site (Arg277) as well as the NH2 and COOH termini of ovine PGHS-1 are on the luminal side of the ER. 19864504 9 35 gly ASA 1267:1269 arg1 the highest mannose-6-phosphate content ASA the highest mannose-6-phosphate content OGER ASA P15289 CHO cells cultured under bioreactor conditions yielded recombinant ASA with the most preserved N-glycan structures, the highest mannose-6-phosphate content and the highest similarity to non-recombinant enzyme. 19864504 9 35 gly ASA 1267:1269 arg1 the most preserved N-glycan structures ASA the most preserved N-glycan structures OGER ASA P15289 CHO cells cultured under bioreactor conditions yielded recombinant ASA with the most preserved N-glycan structures, the highest mannose-6-phosphate content and the highest similarity to non-recombinant enzyme. 10988251 12 6 gly alpha1m 1832:1838 arg1 the carbohydrates alpha1m the carbohydrates PUBTATOR alpha1m 252922 It is concluded that the carbohydrates of alpha1m are important for the secretion and the in vivo turnover of the protein, but not for the structure or immunological properties. 22288421 6 51 gly glycosylation 986:998 arg1 CD45 CD45 PUBTATOR CD45 5788 This review focuses on changes in glycosylation of CD43 and CD45 occurring throughout T cell development and activation and the role that glycosylation plays in regulating T cell processes, such as migration, T cell receptor signaling, and apoptosis. 22288421 6 51 gly glycosylation 986:998 arg1 CD43 CD43 PUBTATOR CD43 6693 This review focuses on changes in glycosylation of CD43 and CD45 occurring throughout T cell development and activation and the role that glycosylation plays in regulating T cell processes, such as migration, T cell receptor signaling, and apoptosis. 8660696 4 2 gly O-glycosylation 584:598 arg1 APP APP Cterm APP Since glycosylation can affect many properties of glycoproteins, we studied the role of N- and O-glycosylation in the synthesis and secretion of APP. 29932112 4 17 gly N-glycosylation 547:561 arg1 Panx2 Panx2 PUBTATOR Panx2 56666 Our objectives were to validate the predicted N-glycosylation site of Panx2 and to study the effects of Panx2 glycosylation on localization and its capacity to interact with Panx1. 15530432 0 42 gly P-glycoprotein 110:123 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Thapsigargin or curcumin does not promote maturation of processing mutants of the ABC transporters, CFTR, and P-glycoprotein. 1715920 4 40 gly hCG 849:851 arg1 the carbohydrate (CHO) units hCG the carbohydrate (CHO) units PUBTATOR hCG 93659 Thus, the carbohydrate (CHO) units of hCG neither seem to be part of these 14 antigenic sites nor to contribute to the affinity of receptor binding: both variants had even higher affinities than native hCG. 9115255 1 24 gly glycoprotein 274:285 arg1 Bovine adrenal medullary chromogranin A Bovine adrenal medullary chromogranin A PUBTATOR chromogranin A 281070 Bovine adrenal medullary chromogranin A, the major soluble component of chromaffin granules, is a phosphorylated glycoprotein. 27313224 0 76 gly N559-glycan 17:27 arg1 the synaptic vesicle glycoprotein 2C synaptic vesicle glycoprotein 2C N559-glycan PUBTATOR synaptic vesicle glycoprotein 2C 22987 Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1. 27313224 0 82 gly glycoprotein 53:64 arg1 the synaptic vesicle glycoprotein 2C the synaptic vesicle glycoprotein 2C PUBTATOR synaptic vesicle glycoprotein 2C 22987 Only the complex N559-glycan in the synaptic vesicle glycoprotein 2C mediates high affinity binding to botulinum neurotoxin serotype A1. 6773934 2 18 gly glycosylation 403:415 arg1 the beta subunit the beta subunit OGER subunit P0DN86 beta-Hydroxynorvaline inhibited the glycosylation of the alpha subunit of human chorionic gonadotropin and the beta subunit of bovine luteinizing hormone; both proteins contain Asn-X-Thr recognition sites. 16331960 3 68 gly nonglycosylated 607:621 arg1 normal glycosylated and nonglycosylated recombinant human transferrin normal glycosylated and nonglycosylated recombinant human transferrin PUBTATOR transferrin 7018 Differential susceptibility to proteolysis by chymotrypsin was demonstrated for normal glycosylated and nonglycosylated recombinant human transferrin, using reverse-phase (RP) HPLC, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and LC-tandem mass spectrometry (MS/MS). 16331960 3 71 gly glycosylated 590:601 arg1 normal glycosylated and nonglycosylated recombinant human transferrin normal glycosylated and nonglycosylated recombinant human transferrin PUBTATOR transferrin 7018 Differential susceptibility to proteolysis by chymotrypsin was demonstrated for normal glycosylated and nonglycosylated recombinant human transferrin, using reverse-phase (RP) HPLC, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and LC-tandem mass spectrometry (MS/MS). 19267675 6 56 gly Deglycosylated 820:833 arg1 sCD4 sCD4 PUBTATOR sCD4 79966 Deglycosylated forms of sCD4 in vivo acquire a specific conformation similar to the wild type sCD4, which however cannot be restored after denaturation/renaturation under conditions of non-reducing Western blot. 16467306 2 40 gly glycoprotein 222:233 arg1 MD-2 MD-2 PUBTATOR MD-2 23643 MD-2 is a 20-25-kDa extracellular glycoprotein that binds to Tolllike receptor 4 (TLR4) and LPS and is a critical part of the LPS receptor. 8652881 8 59 gly glycosylated 1472:1483 arg1 recombinant FVIII-delta II recombinant FVIII-delta II OGER FVIII P00451 The difference from the theoretical molecular molecular masses and the observation of broad molecular peaks suggest that recombinant FVIII-delta II has been effectively glycosylated by the host cell on both heavy and light chains. 2026707 0 12 gly interleukin-3 114:126 arg1 oligosaccharide composition interleukin-3 oligosaccharide composition PUBTATOR interleukin-3 16187 Mass spectrometric determination of glycosylation sites and oligosaccharide composition of insect-expressed mouse interleukin-3. 15047148 5 10 gly hypoglycosylated 696:711 arg1 hypoglycosylated CD28 hypoglycosylated CD28 PUBTATOR CD28 940 Stimulation of hypoglycosylated CD28 induced IL-2 promoter activity greater than that induced through the stimulation of wildtype CD28. 10725420 8 1 gly structures 1650:1659 arg1 gp120/gp41 gp120 structures PUBTATOR gp120 3700 This study shows that PI of HIV bind to MBL and suggests that MBL can selectively interact with HIV in vivo via carbohydrate structures on gp120/gp41. 27818199 8 79 gly underglycosylated 1136:1152 arg1 an underglycosylated form form of ACE PUBTATOR form of ACE 1636 More importantly, it affected the processing of the protein to the membrane, resulting in expression of an underglycosylated form of ACE. 9774483 2 49 gly NCAM 541:544 arg1 polysialylation NCAM polysialylation PUBTATOR NCAM 4684 To determine the individual and combined roles of PST and STX in polysialic acid synthesis, in the present study we asked if PST and STX differ in the acceptor requirement and if PST and STX act together in polysialylation of NCAM. 9774483 2 63 gly polysialylation 522:536 arg1 NCAM NCAM PUBTATOR NCAM 4684 To determine the individual and combined roles of PST and STX in polysialic acid synthesis, in the present study we asked if PST and STX differ in the acceptor requirement and if PST and STX act together in polysialylation of NCAM. 8943261 0 58 gly glycosylation 27:39 arg1 murine acid sphingomyelinase murine acid sphingomyelinase PUBTATOR acid sphingomyelinase 20597 Functional analysis of the glycosylation of murine acid sphingomyelinase. 10218949 2 19 gly glycosylated 492:503 arg1 the endogenous AT1-R the endogenous AT1-R PUBTATOR AT1-R 24180 All three asparagine residues (Asn4, Asn176, Asn188) contained within consensus sites for N-linked glycosylation could be glycosylated in Cos-7 cells and appeared to be glycosylated on the endogenous AT1-R in bovine adrenal glomerulosa cells. 16669630 8 47 gly Deglycosylation 1254:1268 arg1 aspartoacylase aspartoacylase OGER aspartoacylase P45381 Deglycosylation of aspartoacylase or mutation at the glycosylation site causes decreased enzyme stability and diminished catalytic activity. 29533934 2 23 gly glycoprotein 361:372 arg1 gp130 gp130 OGER gp130 P40189 It activates its target cells through binding to the IL-11 receptor (IL-11R), and the IL-11/IL-11R complex recruits a homodimer of glycoprotein 130 (gp130). 21709263 3 10 gly probe 441:445 arg1 CL1-0 CL1 probe PUBTATOR CL1 22859 In the current study, we have identified sialylated proteins using an alkynyl sugar probe in two different lung cancer cell lines, CL1-0 and CL1-5 with distinct invasiveness derived from the same parental cell line. 14970177 0 42 gly glycosylation 9:21 arg1 Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6 Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6 OGER interleukin 6 P05231 N-linked glycosylation is required for optimal function of Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6. 16401092 8 48 part_of Thr-155 1305:1311 arg1 mZP3 mZP3 Thr-155 PUBTATOR SpecificSite mZP3 22788 Thr-155 However, in huZP3 derived from rescue mice, the O-glycans associated with Thr-156 (analogous to Thr-155 in mZP3) are exclusively core 1 and related Tn sequences, whereas core 2 O-glycans predominate at the other conserved site. 14670950 5 9 gly glycosylation 1102:1114 arg1 MT1-MMP MT1-MMP PUBTATOR MT1-MMP 4323 Although the autolytic processing and interstitial collagenase activity of MT1-MMP were not impaired in glycosylation-deficient mutants, cell surface MT1-MMP-catalyzed activation of pro-matrix metalloproteinase-2 (proMMP-2) required proper glycosylation of MT1-MMP. 6175959 10 24 part_of C3d 1196:1198 arg1 1-49 C3d 1-49 PUBTATOR SpecificSite C3d 100861467 residues 1-49 Comparison of residues 1-49 of C3d with a peptide from alpha(2)-macroglobulin [Swenson, R. P. & Howard, J. B. (1980) J. Biol. 27313224 11 19 gly SV2C-LD4 1839:1846 arg1 the complex N559-glycan SV2C the complex N559-glycan PUBTATOR SV2C 22987 In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding a third anchor point beside a ganglioside and the SV2C-LD4 peptide, for BoNT/A neuronal cell surface binding and uptake. 20800224 4 2 gly N-glycosylated 546:559 arg1 mutant (C87S) recombinant NGAL mutant (C87S) recombinant NGAL PUBTATOR NGAL 3934 This study demonstrated that 7-9% of mutant (C87S) recombinant NGAL was N-glycosylated and no O-glycosylation was detected. 26291458 9 15 gly sites 1260:1264 arg1 Drosophila Smo Smo sites PUBTATOR Smo 33196 Of the seven predicted glycan acceptor sites in Drosophila Smo, one is essential. 11923845 3 11 part_of p40 467:469 arg1 N222 p40 N222 PUBTATOR SpecificSite p40 3578 N222 Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. 11923845 3 11 part_of p40 467:469 arg1 N220 p40 N220 PUBTATOR SpecificSite p40 3578 N220 Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. 11923845 3 34 part_of p40 445:447 arg1 N222 p40 N222 PUBTATOR SpecificSite p40 16160 N222 Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. 11923845 3 34 part_of p40 445:447 arg1 N220 p40 N220 PUBTATOR SpecificSite p40 16160 N220 Here, we found that mutations introduced into N-glycosylation sites (N220 of murine p40 and N222 of human p40) reduced secretion of p40 but not p70. 17117926 0 39 gly glycosylation 38:50 arg1 Wnt-5a Wnt-5a PUBTATOR Wnt-5a 7474 Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling. 7612933 1 69 gly glycoprotein 213:224 arg1 glycoprotein D glycoprotein D OGER glycoprotein D Q16570 An infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1) gene homologous to glycoprotein D of herpes simplex virus (HSV) was identified and characterized by its nucleotide and derived amino acid sequence. 27175940 2 8 gly glycosylation 451:463 arg1 p27 p27 PUBTATOR p27 3429 The post-translational addition of O-GlcNAc to p27 occurs in HEK293T and HCC (hepatocellular carcinoma) cell lines, and we identified Ser2, Ser106, Ser110, Thr157, and Thr198 as the glycosylation sites of p27 based on the Q-TOF spectrum. 20106922 10 21 gly glycosylation 1912:1924 arg1 host PrP host PrP PUBTATOR PrP 19122 This demonstrates that targeting in the brain following peripheral inoculation is profoundly influenced by the glycosylation status of host PrP. 8730100 1 12 gly sialylated 166:175 arg1 Episialin Episialin PUBTATOR Episialin 17829 Episialin (MUC1, PEM, EMA, CA15-3 antigen) is a sialylated, membrane-associated glycoprotein with an extended mucin-like ectodomain. 8730100 1 36 gly glycoprotein 198:209 arg1 Episialin Episialin PUBTATOR Episialin 17829 Episialin (MUC1, PEM, EMA, CA15-3 antigen) is a sialylated, membrane-associated glycoprotein with an extended mucin-like ectodomain. 27725718 9 28 gly glycosylation 1159:1171 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Furthermore, we observed, that glycosylation sites of liver-originating transferrin and haptoglobin are differentially occupied under physiological conditions, a further instance not noticed in serum proteins to date. 27725718 9 28 gly glycosylation 1159:1171 arg1 liver-originating transferrin liver-originating transferrin PUBTATOR transferrin 7018 Furthermore, we observed, that glycosylation sites of liver-originating transferrin and haptoglobin are differentially occupied under physiological conditions, a further instance not noticed in serum proteins to date. 10211957 0 56 gly glycosylation 16:28 arg1 hepatitis C virus (HCV) glycoprotein E1 hepatitis C virus (HCV) glycoprotein E1 Cterm E1 Analysis of the glycosylation sites of hepatitis C virus (HCV) glycoprotein E1 and the influence of E1 glycans on the formation of the HCV glycoprotein complex. 2571506 5 21 part_of Asn 826:828 arg1 CDR CDR)2 Asn PUBTATOR SpecificSite CDR)2 1039 Asn 58 The VH441 gene segment and all seven mAb contain a potential glycosylation site at Asn 58 in complementarity-determining region (CDR)2. 17868453 16 58 gly glycosylated 2209:2220 arg1 Horse PSGL-1 Horse PSGL-1 PUBTATOR Horse PSGL-1 6404 Horse PSGL-1, glycosylated by human or equine glycosyltransferases, did not interact with P-selectin. 17522218 1 57 gly glycans 225:231 arg1 E2 E2 glycans PUBTATOR E2 6044 Hepatitis C virus (HCV) envelope glycoproteins are highly glycosylated, with up to 5 and 11 N-linked glycans on E1 and E2, respectively. 16510764 9 24 gly O-glycans 1186:1194 arg1 IgAN IgAN O-glycans PUBTATOR IgAN 60498 This undergalactosylation was not shared by IgD; in contrast, IgD carried more galactosylated O-glycans in IgAN than controls. 20153530 7 65 gly glycosylated 1130:1141 arg1 this glycosylated C9 this glycosylated C9 PUBTATOR C9 117512 Again, this glycosylated C9 was as active as native C9 and could be induced to polymerize by heating or incubation with metal ions. 11278680 8 1 gly Deglycosylated 1115:1128 arg1 Deglycosylated glycodelin Deglycosylated glycodelin PUBTATOR Deglycosylated glycodelin 5047 Deglycosylated glycodelin was equipotent to intact glycodelin in the monocyte migration assay. 11275255 6 43 gly glycoforms 1026:1035 arg1 IgG1-Fc IgG1-Fc OGER IgG1 P01857 Differential scanning microcalorimetry has been used to compare the stabilities of the homogeneous glycoforms of IgG1-Fc. 10400671 9 60 gly repeats 1279:1285 arg1 fibulin-1 fibulin-1 repeats PUBTATOR fibulin-1 2192 Using various deletion mutants, the binding site for aggrecan and versican lectin domains was mapped to the epidermal growth factor-like repeats in domain II of fibulin-1. 3816803 6 82 gly contains 1119:1126 arg1 SPARC AND a diantennary complex type SPARC a diantennary complex type PUBTATOR SPARC 20692 By contrast to colligin, SPARC secreted by PYS cells contains predominantly a diantennary complex type of chain containing a variable number of sialic acid and core-substituted fucose residues. 12090474 0 43 gly MUC1 35:38 arg1 Non-glycosylated tandem repeats MUC1 Non-glycosylated tandem repeats PUBTATOR MUC1 4582 Non-glycosylated tandem repeats of MUC1 facilitate attachment of breast tumor cells to normal human lung tissue and immobilized extracellular matrix proteins (ECM) in vitro: potential role in metastasis. 15456735 6 89 gly released 968:975 arg1 MUC1(1.7TR)-IgG2a AND The O-linked oligosaccharides MUC1(1.7TR)-IgG2a The O-linked oligosaccharides Cterm IgG2a The O-linked oligosaccharides were released from MUC1(1.7TR)-IgG2a and analyzed by nano-LC-MS and LC-MS/MS. 11874698 1 69 gly glycoforms 226:235 arg1 hCG hCG OGER hCG Human chorionic gonadotropin (hCG) glycoforms change as pregnancy progresses. 11874698 1 69 gly glycoforms 226:235 arg1 Human chorionic gonadotropin Human chorionic gonadotropin OGER chorionic gonadotropin Human chorionic gonadotropin (hCG) glycoforms change as pregnancy progresses. 25009769 4 26 gly N-glycosylation 449:463 arg1 HYAL1 HYAL1 OGER HYAL1 Q12794 N-glycosylation of HYAL1 is important for secretion of HYAL1, as demonstrated by site-directed mutation. 20511397 1 7 gly glycoprotein 209:220 arg1 Apolipoprotein E Apolipoprotein E PUBTATOR Apolipoprotein E 348 Apolipoprotein E (apoE) is a 34-kDa glycoprotein secreted from various cells including hepatocytes and macrophages and plays an important role in remnant lipoprotein clearance, immune responses, Alzheimer disease, and atherosclerosis. 29793953 4 134 gly unglycosylated 889:902 arg1 Endogenous NTCP protein Endogenous NTCP protein PUBTATOR Endogenous NTCP protein 6554 Endogenous NTCP protein from differentiated HepaRG cells was unglycosylated despite wild-type coding sequence. 8364023 8 41 part_of LCAT 1217:1220 arg1 Asn20-->Thr LCAT Asn20-->Thr PUBTATOR AminoAcid LCAT 3931 Asn20 The amount secreted, specific activity, and Vmax of LCAT (Asn20-->Thr) were similar to those of the wild-type LCAT. 10928479 2 45 gly glycoprotein 371:382 arg1 GP GP Cterm GP In particular, the assembly and processing of the multisubunit glycoprotein (GP) Ib-IX-V complex, a receptor for von Willebrand factor (vWf) is not fully understood. 3718934 2 31 gly glycosylated 313:324 arg1 calcitonin calcitonin OGER calcitonin P01257 Preliminary evidence has suggested that there are glycosylated forms of calcitonin and its precursor, procalcitonin. 29220102 10 58 gly pollen-allergic 1217:1231 arg1 Artemisia pollen-allergic patients IgE Artemisia pollen-allergic patients OGER IgE P01854 More than 94% of Artemisia pollen-allergic patients had IgE response to this allergen. 25213400 9 5 gly containing 825:834 arg1 MS2 AND one glucuronic acid MS2 one glucuronic acid PUBTATOR MS2 100271694 The most charged glycan is MS2 containing three sulfate groups and one glucuronic acid; whereas the least charged one is the BA2 residue. 18045392 1 9 gly glycoprotein 199:210 arg1 human erythropoietin human erythropoietin PUBTATOR erythropoietin 2056 AIMS: To design and investigate a recombinant expression system producing a therapeutically important glycoprotein, human erythropoietin (rHuEPO), by Pichia pastoris. 25707740 10 50 gly hyperglycosylated 1323:1339 arg1 hCG hCG OGER hCG The invasive extravillous trophoblast also secretes hCG, and in particular like choriocarcinoma cells, hyperglycosylated forms of hCG (hCG-H). 11453640 2 33 gly glycosylation 292:304 arg1 the CD4 receptor the CD4 receptor PUBTATOR CD4 receptor 920 The resulting hybrids showed dual antigenicity, normal glycosylation, and high affinity binding of the CD4 receptor. 8340384 0 46 gly heterogeneity 10:22 arg1 follistatin follistatin OGER follistatin P19883 Molecular heterogeneity of follistatin, an activin-binding protein. 8340384 0 46 gly heterogeneity 10:22 arg1 an activin-binding protein an activin-binding protein OGER activin-binding protein P19883 Molecular heterogeneity of follistatin, an activin-binding protein. 17711303 0 82 gly N-Glycosylation 0:14 arg1 the human kappa opioid receptor the human kappa opioid receptor PUBTATOR kappa opioid receptor 4986 N-Glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway. 26281700 4 8 gly O-glycosylation 591:605 arg1 Fukutin Fukutin PUBTATOR Fukutin 246179 Introduction of 3 cocktailed antisense oligonucleotides(AONs) targeting around these splice sites prevented pathogenic splicing in FCMD patient cells and model mice, and normalized protein production and functions of Fukutin as well as O-glycosylation of α-dystroglycan. 7523405 14 66 gly glycosylation 2139:2151 arg1 the serotonin transporter the serotonin transporter PUBTATOR serotonin transporter 25553 These data indicate that glycosylation is required for optimal stability of the serotonin transporter in the membrane but not for serotonin transport or ligand binding per se. 15946216 1 1 gly glycosylated 145:156 arg1 Coagulation factor VIII Coagulation factor VIII PUBTATOR Coagulation factor VIII 14069 BACKGROUND: Coagulation factor VIII (FVIII) is a heavily glycosylated heterodimeric plasma protein that consists of a heavy (domains A1-A2-B) and light chain (domains A3-C1-C2). 2536708 4 0 gly subunit 634:640 arg1 a single oligosaccharide subunit a single oligosaccharide OGER subunit P0DN86 Similarly, absence of the N-linked oligosaccharides from the beta subunit or a single oligosaccharide from Asn-78 of alpha had no effect on the production of cAMP or on steroidogenesis. 2536708 4 0 gly subunit 634:640 arg1 the N-linked oligosaccharides subunit the N-linked oligosaccharides OGER subunit P0DN86 Similarly, absence of the N-linked oligosaccharides from the beta subunit or a single oligosaccharide from Asn-78 of alpha had no effect on the production of cAMP or on steroidogenesis. 2536708 4 44 gly oligosaccharide 654:668 arg1 the beta subunit subunit oligosaccharide OGER subunit P0DN86 Similarly, absence of the N-linked oligosaccharides from the beta subunit or a single oligosaccharide from Asn-78 of alpha had no effect on the production of cAMP or on steroidogenesis. 8349699 10 4 gly N-glycosylation 1444:1458 arg1 PGH synthase-1 PGH synthase-1 PUBTATOR PGH synthase-1 19224 Assuming that the N-glycosylation sites of PGH synthase-1 are on the luminal side of the endoplasmic reticulum (ER), and that the site of tryptic cleavage of ovine PGH synthase-1 (Arg277) is on the cytoplasmic side of the ER, we propose that both the NH2 and COOH termini of PGH synthase-1 are located in the lumen of the ER and that there are two transmembrane domains located between Asn144 and Arg277 and between Arg277 and Asn410, respectively. 1637954 5 21 gly deglycosylated 878:891 arg1 deglycosylated hCG deglycosylated hCG OGER hCG The binding of deglycosylated hCG to both membrane preparations and the binding of native hCG to light-membrane preparations was maximal at approximately the same NaCl concentration (50-65 mM). 29405331 3 78 gly glycoprotein 428:439 arg1 A1AT A1AT PUBTATOR A1AT 5265 The serum glycoprotein alpha-1-antitrypsin (A1AT) which contains both core- and antennary-fucosylated glycosites was used in this study. 7999071 0 14 gly glycosylation 98:110 arg1 human lipoprotein lipase human lipoprotein lipase PUBTATOR lipoprotein lipase 4023 A naturally occurring mutation at the second base of codon asparagine 43 in the proposed N-linked glycosylation site of human lipoprotein lipase: in vivo evidence that asparagine 43 is essential for catalysis and secretion. 21511948 2 77 gly glycosylation 327:339 arg1 RAGE RAGE PUBTATOR RAGE 177 N-Linked glycosylation of RAGE plays an important role in the regulation of ligand binding. 29944110 8 31 part_of HA1 1157:1159 arg1 HA1 position N158 HA1 HA1 position N158 PUBTATOR SpecificSite HA1 23526 position N158 We found that clade 3C.2a viruses, possessing an additional potential glycosylation site at HA1 position N158, were poorly recognized by some of the mAbs, but other residues, notably at position 159, also affected antibody binding. 18204788 6 16 gly glycosylated 1186:1197 arg1 sPrP sPrP PUBTATOR PrP 5621 Characterization by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tunicamycin treatment revealed that a fully glycosylated form of sPrP was secreted from the cells. 18707547 2 87 gly glycosylated 407:418 arg1 CD6 CD6 PUBTATOR CD6 451230 CD6, a highly glycosylated membrane protein predominantly expressed on lymphocytes, contains three SRCR domains. 25614217 3 36 gly aglycosylated 449:461 arg1 aglycosylated CTB aglycosylated CTB PUBTATOR CTB 74245 Here, we found that overexpression of aglycosylated CTB by agroinfiltration of a tobamoviral vector causes massive tissue necrosis and poor accumulation unless retained in the endoplasmic reticulum (ER). 21770429 11 17 gly glycosylated 1591:1602 arg1 fully glycosylated IL5Rα fully glycosylated IL5Rα PUBTATOR IL5Rα, 3568 This mostly deglycosylated variant had the same ligand binding affinity and biological activity as fully glycosylated IL5Rα, thus demonstrating a unique role for Asn(196) glycosylation in IL5Rα function. 14702339 10 39 part_of TPP 1747:1749 arg1 Asn-210 TPP I Asn-210 and Asn-286 PUBTATOR SpecificSite TPP I 1200 Asn-210 and Asn-286 Phospholabeling experiments revealed that N-glycans at Asn-210 and Asn-286 of TPP I preferentially accept a phosphomannose marker. 17868453 0 124 gly glycoprotein 40:51 arg1 P-selectin glycoprotein ligand-1 P-selectin glycoprotein ligand-1 PUBTATOR P-selectin glycoprotein ligand-1 6404 Evolutionary conservation of P-selectin glycoprotein ligand-1 primary structure and function. 26961877 5 20 gly PAM-1 880:884 arg1 O-glycan-depleted mutant PAM O-glycan-depleted mutant PUBTATOR PAM 18484 PAM-1 lacking both glycosylation sites (PAM-1/OSX; where OSX is O-glycan-depleted mutant of PAM-1) was stably expressed in AtT-20 corticotrope tumor cells. 2001369 0 41 gly CD4 53:55 arg1 Carbohydrate structures CD4 Carbohydrate structures PUBTATOR CD4 920 Carbohydrate structures of recombinant soluble human CD4 expressed in Chinese hamster ovary cells. 23507963 2 37 gly CLEC10A 400:406 arg1 the carbohydrate specificity CLEC10A the carbohydrate specificity PUBTATOR CLEC10A 10462 In the present study, we have analyzed the carbohydrate specificity of the C-type lectin CLEC10A using glycan profiling by enzyme-linked immunosorbent assay (ELISA). 18068104 1 1 gly glycoprotein 211:222 arg1 THP THP OGER THP P07911 Tamm-Horsfall glycoprotein (THP) is synthesized in the particular sites of renal tubules acting as a defense molecule in the urinary system. 18068104 1 1 gly glycoprotein 211:222 arg1 Tamm-Horsfall glycoprotein Tamm-Horsfall glycoprotein OGER Tamm-Horsfall glycoprotein P07911 Tamm-Horsfall glycoprotein (THP) is synthesized in the particular sites of renal tubules acting as a defense molecule in the urinary system. 8098269 8 52 gly P-glycoprotein 943:956 arg1 P-glycoprotein size P-glycoprotein size PUBTATOR P-glycoprotein 5243 The variation of P-glycoprotein size in the RCC was attributed to differential N-linked glycosylation. 9341152 4 84 gly nonglycosylated 723:737 arg1 proLPC proLPC Cterm proLPC Analysis of expression of recombinant LPC in stably transfected Chinese hamster ovary cells reveals biosynthesis of a 92-kDa nonglycosylated precursor (proLPC) and a 102-kDa endoglycosidase H-sensitive glycosylated form of proLPC. 9341152 4 46 gly glycosylated 800:811 arg1 a 102-kDa endoglycosidase H-sensitive glycosylated form form of proLPC Cterm form of proLPC Analysis of expression of recombinant LPC in stably transfected Chinese hamster ovary cells reveals biosynthesis of a 92-kDa nonglycosylated precursor (proLPC) and a 102-kDa endoglycosidase H-sensitive glycosylated form of proLPC. 10383441 4 35 part_of contains 571:578 arg1 murine DNase I AND Lys27 DNase I Lys27 and Lys74 PUBTATOR AminoAcid DNase I 13419 Lys27 and Lys74 We now demonstrate that murine DNase I, which contains Lys27 and Lys74, is phosphorylated only 20.9% when expressed in the same COS-1 cell system. 12505154 6 56 gly glycosylation 918:930 arg1 the MCHR1 the MCHR1 PUBTATOR MCHR1 83567 These data outline the importance of the N-linked glycosylation of the MCHR1. 17823199 6 35 gly -antitrypsin 1131:1142 arg1 all N-glycosylation sites alpha(1)-antitrypsin all N-glycosylation sites PUBTATOR alpha(1)-antitrypsin 5265 In healthy control samples, we determined 98-100% occupancy for all N-glycosylation sites of transferrin and alpha(1)-antitrypsin. 17823199 6 45 gly transferrin 1107:1117 arg1 all N-glycosylation sites transferrin all N-glycosylation sites PUBTATOR transferrin 7018 In healthy control samples, we determined 98-100% occupancy for all N-glycosylation sites of transferrin and alpha(1)-antitrypsin. 17823199 6 52 gly N-glycosylation 1082:1096 arg1 transferrin transferrin PUBTATOR transferrin 7018 In healthy control samples, we determined 98-100% occupancy for all N-glycosylation sites of transferrin and alpha(1)-antitrypsin. 17823199 6 52 gly N-glycosylation 1082:1096 arg1 alpha(1)-antitrypsin alpha(1)-antitrypsin PUBTATOR alpha(1)-antitrypsin 5265 In healthy control samples, we determined 98-100% occupancy for all N-glycosylation sites of transferrin and alpha(1)-antitrypsin. 18190944 7 59 gly glycosylated 1072:1083 arg1 the M2L protein the M2L protein PUBTATOR M2L protein 3707646 Indeed, the M2L protein was demonstrated to be N-linked glycosylated and expressed early during infection. 22178065 10 12 gly non-glycosylated 1495:1510 arg1 recombinant non-glycosylated PAI-1 recombinant non-glycosylated PAI-1 PUBTATOR PAI-1 5054 Our data also suggest that PAI-1 inhibitors for use in humans must preferably be screened on glycosylated PAI-1 and not on recombinant non-glycosylated PAI-1. 22178065 10 34 gly glycosylated 1453:1464 arg1 glycosylated PAI-1 glycosylated PAI-1 PUBTATOR PAI-1 5054 Our data also suggest that PAI-1 inhibitors for use in humans must preferably be screened on glycosylated PAI-1 and not on recombinant non-glycosylated PAI-1. 11275255 0 56 gly glycosylation 17:29 arg1 human IgG1-Fc human IgG1-Fc OGER IgG1 P01857 The influence of glycosylation on the thermal stability and effector function expression of human IgG1-Fc: properties of a series of truncated glycoforms. 7780192 7 43 gly IgM 1700:1702 arg1 the oligosaccharides IgM the oligosaccharides OGER IgM P01872 Of note is the presence of N-glycolylneuraminic acid (NeuGc) and N-acetylneuraminic acid (NeuAc) at a 2:1 ratio in the oligosaccharides of the human hybridoma IgM. 17715132 9 40 gly N-glycosylation 1149:1163 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We propose that N-glycosylation of Pannexin1 could be a significant mechanism for regulating the trafficking of these membrane proteins to the cell surface in different tissues. 24179160 1 11 gly glycoprotein 100:111 arg1 Env Env PUBTATOR Env 155971 The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and membrane fusion machinery that introduce the viral genome into the host cell. 24179160 1 11 gly glycoprotein 100:111 arg1 HIV-1 envelope glycoprotein HIV-1 envelope glycoprotein PUBTATOR HIV-1 envelope glycoprotein 155971 The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and membrane fusion machinery that introduce the viral genome into the host cell. 7741718 5 31 part_of IP3R 1057:1060 arg1 serine-1755 IP3R serine-1755 PUBTATOR SpecificSite IP3R 25262 serine-1755 According to current models of the cerebellar IP3R this would place the proteolytic site between the phosphorylation site at serine-1755 and the first transmembrane segment of the IP3R. 17144900 5 81 gly glycans 823:829 arg1 C C glycans Cterm C P32119 A better understanding of the nature of the N-linked glycans on PrP(C) during the normal aging process may provide new insights into the roles that N-linked glycans play in the pathogenesis of prion diseases. 17144900 5 81 gly glycans 823:829 arg1 PrP PrP glycans OGER PrP P32119 A better understanding of the nature of the N-linked glycans on PrP(C) during the normal aging process may provide new insights into the roles that N-linked glycans play in the pathogenesis of prion diseases. 9049331 1 48 gly glycoproteins 149:161 arg1 E2 E2 Cterm E2 The maturation of rubella virus (RV) glycoproteins E2 and E1 was examined by using brefeldin A (BFA) and monensin. 8323299 0 39 gly glycoproteins 94:106 arg1 lamp-2 lamp-2 PUBTATOR lamp-2 3920 Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2. 8323299 0 39 gly glycoproteins 94:106 arg1 lamp-1 lamp-1 PUBTATOR lamp-1 3916 Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2. 10353820 6 52 gly nonfucosylated 956:969 arg1 the nonfucosylated and fucosylated FVII EGF-1 the nonfucosylated and fucosylated FVII EGF-1 OGER FVII EGF-1 P08709 The Ca2+ dissociation constants (Kd) for the nonfucosylated and fucosylated FVII EGF-1 were found to be 16.4 +/- 1.8 and 8.6 +/- 1.4 mM, respectively. 10353820 6 96 gly fucosylated 975:985 arg1 the nonfucosylated and fucosylated FVII EGF-1 the nonfucosylated and fucosylated FVII EGF-1 OGER FVII EGF-1 P08709 The Ca2+ dissociation constants (Kd) for the nonfucosylated and fucosylated FVII EGF-1 were found to be 16.4 +/- 1.8 and 8.6 +/- 1.4 mM, respectively. 23700164 10 10 gly glycosylated 1340:1351 arg1 the highly glycosylated GLUT4 the highly glycosylated GLUT4 PUBTATOR GLUT4 6517 Besides, topology mapping of 12 transmembrane-helixes was done to predict N- and O-glycosylation sites and to show the highly glycosylated GLUT4 that includes both N- and O-glycosylation sites. 10548047 1 17 gly N-glycosylation 170:184 arg1 HBP HBP PUBTATOR HBP 566 The three N-glycosylation sites of human heparin binding protein (HBP) have been mutated to produce a nonglycosylated HBP (ng-HBP) mutant. 10548047 1 17 gly N-glycosylation 170:184 arg1 human heparin binding protein human heparin binding protein PUBTATOR heparin binding protein 566 The three N-glycosylation sites of human heparin binding protein (HBP) have been mutated to produce a nonglycosylated HBP (ng-HBP) mutant. 25512553 7 61 part_of SERT 1115:1118 arg1 the SERT glycosylation sites SERT the SERT glycosylation sites PUBTATOR AminoAcid SERT 6532 sites, Asp208 Furthermore, whereas ERp44 constitutively occupies Cys200/Cys209 residues, one of the SERT glycosylation sites, Asp208 located between the two Cys residues, cannot undergo proper glycosylation, which plays an important role in the uptake efficiency of SERT. 2438169 2 18 gly has 225:227 arg1 hCG AND four O-linked sugar units hCG four O-linked sugar units PUBTATOR hCG 1081 hCG has four O-linked sugar units, all attached to the beta-subunit. 2438169 2 18 gly has 225:227 arg1 hCG AND all hCG all PUBTATOR hCG 1081 hCG has four O-linked sugar units, all attached to the beta-subunit. 23005037 9 41 gly N-glycosylation 1456:1470 arg1 CD147 CD147 PUBTATOR CD147 682 The present study reveals the important role of N-glycosylation of CD147 in its biological function and implied that targeting aberrant β1,6-branching of N-glycans on CD147 would be valuable for the development of novel therapeutic modalities against carcinoma. 19916043 5 51 part_of residue 600:606 arg1 the VP7 VP7 residue PUBTATOR SpecificSite VP7 3773131 residue 67 Similar to other human group C rotaviruses, one N-glycosylation site was predicted at amino acid residue 67 on the VP7 of strain GUP188. 27604319 4 0 gly glycosylation 713:725 arg1 gp120 gp120 PUBTATOR gp120 155971 Knowing the site-specific glycosylation of gp120 can facilitate the rational design of glycopeptide antigens for HIV vaccine development. 11846800 5 60 gly HCII 892:895 arg1 Proximal alpha 1-->6 fucosylation HCII Proximal alpha 1-->6 fucosylation OGER HCII P05546 Proximal alpha 1-->6 fucosylation of oligosacharides from Chinese hamster ovary cell-derived HCII was detected in > 90% of the diantennary and triantennary glycans, the latter being slightly less sialylated with exclusively alpha 2-->3-linked N-acetylneuraminic acid units. 11846800 5 68 gly fucosylation 820:831 arg1 Chinese hamster ovary cell-derived HCII HCII fucosylation OGER HCII P05546 Proximal alpha 1-->6 fucosylation of oligosacharides from Chinese hamster ovary cell-derived HCII was detected in > 90% of the diantennary and triantennary glycans, the latter being slightly less sialylated with exclusively alpha 2-->3-linked N-acetylneuraminic acid units. 20639197 6 64 gly glycosylation 862:874 arg1 β2 subunits β2 subunits PUBTATOR 2 subunits 4760 Although glycosylation of Asn-173 in the Cys-loop was important for stability of β2 subunits when expressed alone, results obtained with flow cytometry, brefeldin A treatment, and endo-β-N-acetylglucosaminidase H digestion suggested that glycosylation of Asn-104 was required for efficient α1β2 receptor assembly and/or stability in the endoplasmic reticulum. 20639197 6 68 gly glycosylation 1091:1103 arg1 efficient α1β2 receptor assembly α1β2 receptor Cterm α1β2 receptor 4760 Although glycosylation of Asn-173 in the Cys-loop was important for stability of β2 subunits when expressed alone, results obtained with flow cytometry, brefeldin A treatment, and endo-β-N-acetylglucosaminidase H digestion suggested that glycosylation of Asn-104 was required for efficient α1β2 receptor assembly and/or stability in the endoplasmic reticulum. 8011427 0 25 part_of Insulin 0:6 arg1 Insulin receptor autophosphorylation sites Insulin Insulin receptor autophosphorylation sites PUBTATOR SpecificSite Insulin 100766818 sites tyrosines 1162 and 1163 Insulin receptor autophosphorylation sites tyrosines 1162 and 1163 control both insulin-dependent and insulin-independent receptor internalization pathways. 14551220 1 15 gly V 265:265 arg1 beta1-6 GlcNAc branching N-acetylglucosaminyltransferase V beta1-6 GlcNAc branching PUBTATOR N-acetylglucosaminyltransferase V 4249 beta1-6 GlcNAc branching, a product of N-acetylglucosaminyltransferase V (GnT-V), is a key structure that is associated with malignant transformations and cancer metastasis. 27681177 6 11 gly glycosylation 1145:1157 arg1 SLC26A6 SLC26A6 PUBTATOR SLC26A6 65010 Biotinylation studies of SLC26A6 glycosylation mutants indicated that glycosylation is not essential for cell surface delivery of SLC26A6 but suggested that it may affect the efficacy with which it is trafficked and maintained in the plasma membrane. 28089369 1 9 gly Notch 300:304 arg1 epidermal growth factor-like (EGF) repeats Notch epidermal growth factor-like (EGF) repeats PUBTATOR Notch 31293 Fringe proteins are β3-N-acetylglucosaminyltransferases that modulate Notch activity by modifying O-fucose residues on epidermal growth factor-like (EGF) repeats of Notch. 15331613 1 48 gly glycosylation 496:508 arg1 NGC NGC PUBTATOR NGC 29873 NGC dramatically changed its structure from a proteoglycan to a nonproteoglycan form with cerebellar development, whereas a small portion of NGC molecules existed in a nonproteoglycan form in the other areas of the mature CNS, suggesting that the CS glycosylation of NGC is developmentally regulated in the whole CNS. 26884342 6 22 gly neuropilin-2 1163:1174 arg1 the O-glycan-containing linker region neuropilin-2 the O-glycan-containing linker region PUBTATOR neuropilin-2 8828 Evaluation of the soluble chimeric proteins demonstrated that the meprin A5 antigen-μ tyrosine phosphatase (MAM) domain and the O-glycan-containing linker region of neuropilin-2 are necessary and sufficient for its polysialylation and serve as better recognition and acceptor sites in the polysialylation process than those regions of neuropilin-1. 12489987 3 7 gly present 451:457 arg2 CXCR4 AND g1 CXCR4 g1 PUBTATOR CXCR4 7852 Here we investigated the influence of the N-linked glycans g1 and g2 present on CXCR4 for HIV-1 infection. 12489987 3 7 gly present 451:457 arg2 CXCR4 AND g2 CXCR4 g2 PUBTATOR CXCR4 7852 Here we investigated the influence of the N-linked glycans g1 and g2 present on CXCR4 for HIV-1 infection. 12489987 3 7 gly present 451:457 arg2 CXCR4 AND the N-linked glycans g1 and g2 CXCR4 the N-linked glycans g1 and g2 PUBTATOR CXCR4 7852 Here we investigated the influence of the N-linked glycans g1 and g2 present on CXCR4 for HIV-1 infection. 20053750 0 66 gly hemagglutinin-neuraminidase 67:93 arg1 N-linked glycan neuraminidase N-linked glycan PUBTATOR neuraminidase 4758 N-linked glycan at residue 523 of human parainfluenza virus type 3 hemagglutinin-neuraminidase masks a second receptor-binding site. 22358666 4 103 gly hormone 536:542 arg1 the oligosaccharides human follicle stimulating hormone the oligosaccharides Cterm human follicle stimulating hormone The specific structures of the oligosaccharides of human follicle stimulating hormone have been shown to influence both thein vitro andin vivo bioactivity. 3048385 2 47 gly glycosylated 345:356 arg1 SGP-1 SGP-1 PUBTATOR SGP-1 25524 Pulse-chase labeling shows that SGP-1 is synthesized as a cotranslationally glycosylated 67-kilodalton (kDa) precursor which is posttranslationally modified to a 70-kDa form before secretion to the extracellular space. 11051459 10 48 gly glycosylation 1415:1427 arg1 beta1-integrins beta1-integrins PUBTATOR beta1 3779 The glycosylation of beta1-integrins on neutrophils may act to hide the ligand-binding site in unstimulated cells thereby contributing to the affinity modulation observed in neutrophil beta1-integrin function. 19581304 1 14 gly P-glycoprotein 135:148 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 P-glycoprotein (P-gp, ATP-binding cassette B1) is a drug pump that extracts toxic drug substrates from the plasma membrane and catalyzes their ATP-dependent efflux. 26828122 4 13 gly glycoprotein 953:964 arg1 the hTPPT protein the hTPPT protein PUBTATOR hTPPT protein 80736 Using several approaches including inhibiting de novo N-glycosylation in human colonic epithelial NCM460 cells with tunicamycin as well as enzymatic de-glycosylation, we show that the hTPPT protein is, indeed, a glycoprotein. 1469058 2 65 gly heterogeneity 300:312 arg1 CD44 CD44 PUBTATOR CD44 12505 However, many questions remain about the molecular heterogeneity of CD44 and mechanisms which control its recognition of this ligand. 6935656 5 24 gly glycosylated 936:947 arg1 gp70 gp70 Cterm gp70 The observed differences in gel electrophoretic mobilities and glycopeptide profiles of the respective glycosylated envelope gene cleavage products (gp70) may be accounted for by the presence of an additional oligosaccharide chain on the gp70 of the GIX- virus. 6935656 5 107 gly presence 1016:1023 arg1 the gp70 AND an additional oligosaccharide chain the gp70 an additional oligosaccharide chain Cterm gp70 The observed differences in gel electrophoretic mobilities and glycopeptide profiles of the respective glycosylated envelope gene cleavage products (gp70) may be accounted for by the presence of an additional oligosaccharide chain on the gp70 of the GIX- virus. 12610150 3 76 gly glycoproteins 453:465 arg1 gD gD PUBTATOR gD 2532 Coexpression of the four glycoproteins (gD, gB, gH, and gL) promotes cell-cell fusion. 8474154 4 48 gly glycosylated 648:659 arg1 Protein p32 Protein p32 PUBTATOR Protein p32 3622 Protein p32 is not glycosylated in spite of the presence of two putative N-glycosylation sites in the deduced amino acid sequence of the polypeptide. 16412100 4 6 gly APP 588:590 arg1 alpha2,6-sialylation APP alpha2,6-sialylation OGER APP P12023 Here, we report that overexpression of ST6Gal-I in Neuro2a cells enhanced alpha2,6-sialylation of endogenous APP and increased the extracellular levels of its metabolites [Abeta by two-fold, soluble APPbeta (sAPPbeta) by three-fold and sAPPalpha by 2.5-fold). 16412100 4 44 gly alpha2,6-sialylation 553:572 arg1 endogenous APP endogenous APP OGER APP P12023 Here, we report that overexpression of ST6Gal-I in Neuro2a cells enhanced alpha2,6-sialylation of endogenous APP and increased the extracellular levels of its metabolites [Abeta by two-fold, soluble APPbeta (sAPPbeta) by three-fold and sAPPalpha by 2.5-fold). 8394255 11 32 gly deglycosylated 1602:1615 arg1 Chemically deglycosylated oFSH FSH OGER FSH Chemically deglycosylated oFSH (DG-oFSH) was inactive in these cells but it effectively blocked the action of native hormone. 7841792 4 18 gly glycopeptide 653:664 arg1 a mutant human albumin Casebrook a mutant human albumin Casebrook OGER albumin Casebrook P02768 Using a model glycopeptide from a mutant human albumin Casebrook, glycosylated PTH-Asn was recovered after sequential solid-phase Edman degradation, subjected to acid hydrolysis and the sugars were identified by high performance anion exchange chromatography with pulsed amperometric detection. 7841792 4 51 gly glycosylated 705:716 arg1 glycosylated PTH-Asn glycosylated PTH-Asn PUBTATOR AminoAcid PTH 5741 Using a model glycopeptide from a mutant human albumin Casebrook, glycosylated PTH-Asn was recovered after sequential solid-phase Edman degradation, subjected to acid hydrolysis and the sugars were identified by high performance anion exchange chromatography with pulsed amperometric detection. 29231704 7 20 gly glycoprotein 1112:1123 arg1 AGP AGP Cterm AGP We applied this method for the analysis of alpha-1-acid glycoprotein (AGP). 12815060 6 52 gly glycosylation 848:860 arg1 Bves Bves PUBTATOR Bves 408032 First, glycosylation of Bves at exogenous sites within the carboxyl terminus was only observed in a construct that lacked the third membrane domain, which presumably reversed the orientation of the carboxyl terminus. 11150304 2 26 part_of Ser 427:429 arg1 the murine estrogen receptor-beta estrogen receptor-beta Ser OGER SpecificSite estrogen receptor-beta O08537 Ser(16) Recently, we demonstrated that the murine estrogen receptor-beta (mER-beta) is alternatively O-GlcNAcylated or O-phosphorylated at Ser(16). 10951195 2 44 gly O-glycosylation 359:373 arg1 IGFBP-6 IGFBP-6 PUBTATOR IGFBP-6 3489 The effects of O-glycosylation of IGFBP-6 on binding to glycosaminoglycans and proteolysis, both of which reduce the IGF binding affinity of other IGFBPs were studied. 20823119 0 83 gly IgA1 23:26 arg1 O-glycans IgA1 O-glycans PUBTATOR IgA1 3493 Clustered O-glycans of IgA1: defining macro- and microheterogeneity by use of electron capture/transfer dissociation. 7711052 6 9 gly plasminogen 1451:1461 arg1 the N-linked sugar plasminogen the N-linked sugar OGER plasminogen P00747 Additionally, in fibrinolysis, within a ternary complex of fibrin, plasminogen and tissue plasminogen activator, the N-linked sugar of plasminogen hinders the initial interaction with tissue plasminogen activator (i.e., it alters Km). 15522226 2 47 gly glycoprotein 243:254 arg1 The WFS1 protein The WFS1 protein PUBTATOR WFS1 protein 22393 The WFS1 protein is a glycoprotein located in the endoplasmic reticulum (ER) membrane but its function is poorly understood. 11551653 1 9 gly glycoprotein 185:196 arg1 glycoprotein D glycoprotein D OGER glycoprotein D Q9QUI6 Equine herpesvirus 1 glycoprotein D (EHV-1 gD) has been shown in mouse models and in the natural host to have potential as a subunit vaccine, using various expression systems that included Escherichia coli, baculovirus and plasmid DNA. 28531887 11 35 gly N-glycosylation 1425:1439 arg1 MT4-MMP MT4-MMP PUBTATOR MT4-MMP 4326 Moreover, we identified Asn318 as the single N-glycosylation site of MT4-MMP. 22123080 2 62 gly glycosylation 254:266 arg1 CD82 CD82 PUBTATOR CD82 3732 The glycosylation of CD82 has been shown to be involved in a correlative cell adhesion and motility. 16801529 0 66 gly P-glycoprotein 24:37 arg1 ABCB1 ABCB1 PUBTATOR ABCB1 5243 In situ localization of P-glycoprotein (ABCB1) in human and rat brain. 16801529 0 66 gly P-glycoprotein 24:37 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 In situ localization of P-glycoprotein (ABCB1) in human and rat brain. 25755023 2 53 gly glycoprotein 360:371 arg1 the tumor-associated MUC4 glycoprotein the tumor-associated MUC4 glycoprotein PUBTATOR MUC4 glycoprotein 140474 Although several strategies have been developed to explore anti-tumor vaccines based on MUC1 glycopeptides, only few studies have focused on vaccines directed against the tumor-associated MUC4 glycoprotein. 19951703 8 52 gly deglycosylated 1054:1067 arg1 the deglycosylated UGT1A9 the deglycosylated UGT1A9 PUBTATOR UGT1A9 54600 To the contrary, the deglycosylated UGT1A9, which was produced by the treatment with Endo H under the non-denaturing condition, showed the same enzyme kinetics as the control. 26065635 5 5 gly IgG1 834:837 arg1 the glycan molecule IgG1 the glycan molecule Cterm IgG1 The mutant or wild-type glycosyltransferases transfer sugars with a chemical handle to the glycan molecule of IgG1, making the site-specific linking of cargo molecules possible via the chemical handle, and thus making the process an invaluable technique for the production of homogeneous ADCs. 28597972 7 1 gly glycosylation 1083:1095 arg1 mutant GLP-1R mutant GLP-1R PUBTATOR GLP-1R 2740 However, the glycosylation and function of mutant GLP-1R, in which all three sites for N-linked glycosylation were mutated, were not increased with overexpression of SERP1. 16125194 3 69 gly glycosylated 721:732 arg1 recombinant wild-type cathepsin B recombinant wild-type cathepsin B OGER cathepsin B P07858 However, when produced in P. pastoris we found that recombinant wild-type cathepsin B was preferentially secreted as a heterogeneously glycosylated molecule that migrated at 39 kDa, 41 kDa and a smear of >50 kDa on SDS-PAGE, and was susceptible to treatment with Endo H and PGNase F. 1967025 0 37 gly glycosylation 8:20 arg1 human chorionic gonadotropin human chorionic gonadotropin OGER chorionic gonadotropin Altered glycosylation of human chorionic gonadotropin decreases its hormonal activity as determined by cyclic-adenosine 3',5'-monophosphate production in MA-10 cells. 1900431 5 13 gly Fucosylation 599:610 arg1 t-PA t-PA PUBTATOR t-PA 5327 Fucosylation of threonine-61 was observed in t-PA isolated from the Bowes melanoma cell line and from recombinant expression systems using Chinese hamster ovary or human embryonic kidney cells. 1900431 5 32 gly observed 632:639 arg2 t-PA AND Fucosylation t-PA Fucosylation PUBTATOR t-PA 5327 Fucosylation of threonine-61 was observed in t-PA isolated from the Bowes melanoma cell line and from recombinant expression systems using Chinese hamster ovary or human embryonic kidney cells. 19706343 2 103 gly N-glycosylated 328:341 arg1 beta2GPI beta2GPI PUBTATOR beta2GPI 350 beta2GPI is N-glycosylated at several asparagine residues and the glycan moiety conjugated to residue 143 has been proposed to interact with the Gly40-Arg43 motif of beta2GPI. 18268337 4 35 gly glycoproteins 703:715 arg1 GPs GPs OGER GPs Retroviruses pseudotyped with MACV and JUNV but not GTOV glycoproteins (GPs) efficiently used C. callosus TfR1, whereas only JUNV GP could use C. musculinus TfR1. 8601595 8 40 gly glycosylation 1726:1738 arg1 CD44 CD44 PUBTATOR CD44 960 Taken together, these observations indicate that changes in glycosylation of CD44 can have profound effects on its interaction with hyaluronic acid and suggest that glycosylation may provide an important regulatory mechanism of CD44 function. 10406940 1 2 gly glycosylation 141:153 arg1 GC-C C (GC-C PUBTATOR C (GC-C 4883 The role of carbohydrate moieties at the N-linked glycosylation sites of guanylate cyclase C (GC-C), a receptor protein for guanylin, uroguanylin and heat-stable enterotoxin, in ligand binding and structural stability was examined using site-directed mutagenesis of the putative N-linked glycosylation sites in the extracellular domain (ECD) of porcine GC-C. 16720579 4 83 gly C6ST-1 801:806 arg1 N-glycans C6ST-1 N-glycans OGER C6ST-1 Q7LGC8 A nearly complete removal of N-glycans of the recombinant C6ST-1 by peptide N-glycosidase F increased the C6ST activity but decreased the KSST activity. 21613225 0 19 gly phosphorylation 18:32 arg1 LIF LIF phosphorylation PUBTATOR LIF 3976 Extensive mannose phosphorylation on leukemia inhibitory factor (LIF) controls its extracellular levels by multiple mechanisms. 21613225 0 19 gly phosphorylation 18:32 arg1 leukemia inhibitory factor leukemia inhibitory factor phosphorylation PUBTATOR leukemia inhibitory factor 3976 Extensive mannose phosphorylation on leukemia inhibitory factor (LIF) controls its extracellular levels by multiple mechanisms. 16219759 0 43 gly glycosylated 8:19 arg1 Altered glycosylated PrP proteins Altered glycosylated PrP proteins PUBTATOR PrP proteins 19122 Altered glycosylated PrP proteins can have different neuronal trafficking in brain but do not acquire scrapie-like properties. 27655909 5 14 gly deglycosylated 734:747 arg1 deglycosylated SNAT1 deglycosylated SNAT1 PUBTATOR SNAT1 81539 The biotinylation and confocal immunofluorescence analysis showed that the glycosylation-impaired mutants and deglycosylated SNAT1 were equally capable of expressing on the cell surface. 7918455 6 53 gly r-apo 1010:1014 arg1 9, 15, or 18 kringle IV repeats apo(a) 9, 15, or 18 kringle IV repeats PUBTATOR apo(a) 4018 When medium containing r-apo(a) with 9, 15, or 18 kringle IV repeats was mixed with normal human plasma LDL, stable complexes formed that had a bouyant density typical of Lp(a). 10320099 1 20 gly glycoproteins 154:166 arg1 beta-tectorin beta-tectorin PUBTATOR beta-tectorin 6975 The avian and mammalian tectorial membranes both contain two non-collagenous glycoproteins, alpha and beta-tectorin. 9139799 2 99 gly nonglycosylated 460:474 arg1 rLHR rLHR PUBTATOR rLHR 25477 The functional roles of the carbohydrates were analyzed initially through the use of two nonglycosylated receptor mutants rLHR(N(77,152,173,269,277,291)Q) and rLHR(N(77,152,269,277,291)Q;T(175)A). 1549584 7 38 gly glycosylated 1460:1471 arg1 the fully glycosylated gp120 the fully glycosylated gp120 PUBTATOR gp120 3700 We predict that a partially glycosylated gp120 with most of the dispensable N-linked glycosylation sites removed may be a better vaccine candidate than the fully glycosylated gp120. 18698130 0 38 gly DMP1 89:92 arg1 glycosaminoglycan side chain DMP1 glycosaminoglycan side chain PUBTATOR DMP1 13406 Blocking of proteolytic processing and deletion of glycosaminoglycan side chain of mouse DMP1 by substituting critical amino acid residues. 7895905 8 70 gly glycosylated 1270:1281 arg1 the glycosylated pGHR the glycosylated pGHR Cterm pGHR P16882 Therefore, each carbohydrate moiety contributed approximately 10 kDa to the total molecular mass of the pGHR, in sum contributing 30 kDa to the total Mr of the glycosylated pGHR. 19236039 3 78 gly derived 580:586 arg1 bovine rhodopsin AND a membrane protein-compatible 1D4 affinity tag bovine rhodopsin a membrane protein-compatible 1D4 affinity tag PUBTATOR rhodopsin 509933 We describe here an optimized enrichment strategy involving a membrane protein-compatible 1D4 affinity tag that is derived from the carboxy-terminal nine amino residues of bovine rhodopsin, and its corresponding tag-specific, high-affinity monoclonal antibody. 1331527 1 63 gly glycoprotein 150:161 arg1 The human poliovirus receptor The human poliovirus receptor OGER poliovirus receptor P15151 The human poliovirus receptor (hPVR) is a glycoprotein with three immunoglobulin-like extracellular domains, of which the N-terminal domain (V-type domain) is necessary and sufficient for virus binding and uptake. 10716671 0 90 gly glycosylated 47:58 arg1 TFF2 TFF2 PUBTATOR TFF2 7032 The human two domain trefoil protein, TFF2, is glycosylated in vivo in the stomach. 9168962 1 4 gly sialoglycoprotein 203:219 arg1 MG160 MG160 PUBTATOR MG160 29476 MG160, a type I membrane sialoglycoprotein of the medial cisternae of the rat Golgi apparatus, shows high homology (over 90%) with CFR, a fibroblast growth factor receptor, and ESL-1, an E-selectin ligand of the cell surface of murine myeloid cells. 19693772 0 34 gly N-glycosylation 0:14 arg1 ATF6beta ATF6beta PUBTATOR ATF6beta 1388 N-glycosylation of ATF6beta is essential for its proteolytic cleavage and transcriptional repressor function to ATF6alpha. 8407908 2 8 gly glycoprotein 244:255 arg1 Glycoprotein V Glycoprotein V Cterm Glycoprotein V Glycoprotein V (GPV) is a major platelet membrane 82-kDa glycoprotein, missing in the Bernard-Soulier syndrome, that is cleaved when platelets are treated with thrombin. 27511022 6 46 gly Hsp70 1075:1079 arg1 all beneficial properties Hsp70 all beneficial properties PUBTATOR Hsp70 3308 The investigation demonstrated that the modified protein exhibited all beneficial properties of the wild-type Hsp70 and was even superior to the latter for a few parameters. 27834568 5 0 gly glycan 771:776 arg1 H-CDR2 CDR2 glycan PUBTATOR CDR2 1039 We show that an N-linked complex-type Fab glycan in H-CDR2 of 37E1B5 is directly involved in the inhibition of latent TGF-β activation. 1967025 1 32 gly contains 254:261 arg1 Human chorionic gonadotropin AND four mucin-type sugar chains Human chorionic gonadotropin four mucin-type sugar chains OGER chorionic gonadotropin Human chorionic gonadotropin (hCG) purified from pooled urine of normal pregnant women contains four asparagine-linked sugar chains and four mucin-type sugar chains. 1967025 1 32 gly contains 254:261 arg1 Human chorionic gonadotropin AND four asparagine-linked sugar chains Human chorionic gonadotropin four asparagine-linked sugar chains OGER chorionic gonadotropin Human chorionic gonadotropin (hCG) purified from pooled urine of normal pregnant women contains four asparagine-linked sugar chains and four mucin-type sugar chains. 1967025 1 32 gly contains 254:261 arg1 hCG AND four mucin-type sugar chains hCG four mucin-type sugar chains PUBTATOR hCG 93659 Human chorionic gonadotropin (hCG) purified from pooled urine of normal pregnant women contains four asparagine-linked sugar chains and four mucin-type sugar chains. 1967025 1 32 gly contains 254:261 arg1 hCG AND four asparagine-linked sugar chains hCG four asparagine-linked sugar chains PUBTATOR hCG 93659 Human chorionic gonadotropin (hCG) purified from pooled urine of normal pregnant women contains four asparagine-linked sugar chains and four mucin-type sugar chains. 23202518 1 13 gly glycoprotein 154:165 arg1 human cellular prion protein human cellular prion protein PUBTATOR prion protein 19122 The human cellular prion protein (PrP(C)) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. 28920453 4 19 gly O-glycans 623:631 arg1 rhEPOs rhEPOs O-glycans Cterm rhEPOs We developed selective and sensitive method to profile native O-glycans on rhEPOs. 15373830 6 82 gly glycosylated 807:818 arg1 full-length HIC1 proteins full-length HIC1 proteins OGER HIC1 proteins Q14526 Nonglycosylated and glycosylated forms of full-length HIC1 proteins separated by wheat germ agglutinin affinity purification, displayed the same specific DNA-binding activity in electrophoretic mobility shift assays proving that the O-GlcNAc modification is not directly implicated in the specific DNA recognition of HIC1. 15373830 6 84 gly Nonglycosylated 787:801 arg1 full-length HIC1 proteins full-length HIC1 proteins OGER HIC1 proteins Q14526 Nonglycosylated and glycosylated forms of full-length HIC1 proteins separated by wheat germ agglutinin affinity purification, displayed the same specific DNA-binding activity in electrophoretic mobility shift assays proving that the O-GlcNAc modification is not directly implicated in the specific DNA recognition of HIC1. 9751210 1 4 gly glycoprotein 81:92 arg1 Myelin oligodendrocyte glycoprotein Myelin oligodendrocyte glycoprotein PUBTATOR Myelin oligodendrocyte glycoprotein 17441 Myelin oligodendrocyte glycoprotein (MOG) is a protein on the surface of myelin sheaths. 9751210 1 4 gly glycoprotein 81:92 arg1 MOG MOG PUBTATOR MOG 17441 Myelin oligodendrocyte glycoprotein (MOG) is a protein on the surface of myelin sheaths. 27127844 5 75 gly N-glycosylated 591:604 arg1 ATP6V0A4 ATP6V0A4 PUBTATOR ATP6V0A4 50617 Here we ask if human a4 (ATP6V0A4) is N-glycosylated at the predicted site, Asn489. 27127844 5 75 gly N-glycosylated 591:604 arg1 human a4 human a4 Cterm a4 Here we ask if human a4 (ATP6V0A4) is N-glycosylated at the predicted site, Asn489. 9544990 0 83 gly N-glycosylation 0:14 arg1 the prolactin receptor the prolactin receptor PUBTATOR prolactin receptor 5618 N-glycosylation of the prolactin receptor is not required for activation of gene transcription but is crucial for its cell surface targeting. 11904304 1 25 gly glycosylated 263:274 arg1 c-Myc c-Myc PUBTATOR c-Myc 4609 Previously, we reported that c-Myc is glycosylated by O-linked N-acetylglucosamine at Thr-58, a known phosphorylation site and a mutational hot spot in lymphomas. 13678840 2 1 gly P-glycoprotein 303:316 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 The cell-membrane efflux pump, P-glycoprotein (Pgp), appears to contribute to anthelmintic resistance. 13678840 2 1 gly P-glycoprotein 303:316 arg1 Pgp Pgp PUBTATOR Pgp 5243 The cell-membrane efflux pump, P-glycoprotein (Pgp), appears to contribute to anthelmintic resistance. 17208043 4 74 part_of ATX 557:559 arg1 N53 ATX N53 PUBTATOR SpecificSite ATX 18606 N53 and N410 We found that inhibition of N-glycosylation with tunicamycin or by double point deletion of the amino-acids N53 and N410 of ATX inhibit its secretion. 25475176 9 52 gly glycosylates 1794:1805 arg1 CPY* the exogenous reporter mutant carboxypeptidase Y (CPY Cterm the exogenous reporter mutant carboxypeptidase Y (CPY Finally, there is suggestive evidence for N-glycan-dependent ERAD in Trichomonas, which glycosylates and degrades the exogenous reporter mutant carboxypeptidase Y (CPY*). 8349699 6 16 gly glycosylation 850:862 arg1 ovine PGH synthase-1 ovine PGH synthase-1 PUBTATOR PGH synthase-1 19224 N-Glycosylation consensus sequences corresponding to the three glycosylation sites of ovine PGH synthase-1 are conserved in the deduced amino acid sequences of PGH synthases-2. 29090903 4 18 gly glycosylated 595:606 arg1 homogeneously glycosylated insulin homogeneously glycosylated insulin OGER insulin P01308 We report here the first total synthesis of homogeneously glycosylated insulin. 21698221 6 33 gly glycoproteins 1422:1434 arg1 Envs Envs Cterm Envs Subsequent sequence analysis of multiple env clones derived from the R5 HIV-1 isolates revealed that, concomitant with increased TriMab neutralization sensitivity, end-stage R5 variants displayed envelope glycoproteins (Envs) with reduced numbers of potential N-linked glycosylation sites (PNGS), in addition to increased positive surface charge. 21278005 3 13 gly alpha-2-HS-glycoprotein 396:418 arg1 alpha-2-HS-glycoprotein alpha-2-HS-glycoprotein OGER alpha-2-HS-glycoprotein P02765 The bovine proteins alpha-1-acid-glycoprotein (A1AG) and alpha-2-HS-glycoprotein (fetuin A) were spiked in human chronic wound fluids and were subsequently enriched by a boric acid gel affinity chromatography (BAGAC). 21278005 3 13 gly alpha-2-HS-glycoprotein 396:418 arg1 fetuin A fetuin A PUBTATOR fetuin A 280988 The bovine proteins alpha-1-acid-glycoprotein (A1AG) and alpha-2-HS-glycoprotein (fetuin A) were spiked in human chronic wound fluids and were subsequently enriched by a boric acid gel affinity chromatography (BAGAC). 21278005 3 36 gly alpha-1-acid-glycoprotein 359:383 arg1 A1AG A1AG PUBTATOR A1AG 497200 The bovine proteins alpha-1-acid-glycoprotein (A1AG) and alpha-2-HS-glycoprotein (fetuin A) were spiked in human chronic wound fluids and were subsequently enriched by a boric acid gel affinity chromatography (BAGAC). 21278005 3 36 gly alpha-1-acid-glycoprotein 359:383 arg1 alpha-1-acid-glycoprotein alpha-1-acid-glycoprotein PUBTATOR alpha-1-acid-glycoprotein 497200 The bovine proteins alpha-1-acid-glycoprotein (A1AG) and alpha-2-HS-glycoprotein (fetuin A) were spiked in human chronic wound fluids and were subsequently enriched by a boric acid gel affinity chromatography (BAGAC). 7538125 0 66 gly subunit 80:86 arg1 The asparagine-linked oligosaccharides chorionic gonadotropin beta subunit The asparagine-linked oligosaccharides PUBTATOR chorionic gonadotropin beta subunit 1082 The asparagine-linked oligosaccharides of the human chorionic gonadotropin beta subunit facilitate correct disulfide bond pairing. 20823119 2 60 gly IgA1 225:228 arg1 galactose (Gal)-deficient hinge region (HR) O-glycans IgA1 galactose (Gal)-deficient hinge region (HR) O-glycans PUBTATOR IgA1 3493 Aberrantly glycosylated IgA1, with galactose (Gal)-deficient hinge region (HR) O-glycans, plays a pivotal role in the pathogenesis of the disease. 20823119 2 65 gly glycosylated 212:223 arg1 Aberrantly glycosylated IgA1 Aberrantly glycosylated IgA1 PUBTATOR IgA1 3493 Aberrantly glycosylated IgA1, with galactose (Gal)-deficient hinge region (HR) O-glycans, plays a pivotal role in the pathogenesis of the disease. 21763489 5 21 gly Nt-CCR5 950:956 arg1 the main binding determinant CCR5 the main binding determinant OGER CCR5 P51681 Two-dimensional saturation transfer experiments and measurement of relaxation times highlighted Nt-CCR5 residues Y3, V5, P8-T16, E18, I23 and possibly D2 as the main binding determinant. 29268168 1 40 part_of position 199:206 arg1 IgG IgG position Cterm SpecificSite IgG position N297 The binding strength between IgG and FcγR is influenced by the composition of the N-linked glycan at position N297 in the Fc-domain of IgG. 23523791 6 3 gly glycosylation 915:927 arg1 Mef2D Mef2D PUBTATOR Mef2D 17261 Inhibition of the myogenesis-dependent decrease in the glycosylation of Mef2D suppressed its recruitment to the myogenin promoter. 7690959 1 61 gly glycoproteins 283:295 arg1 GPs Ib alpha, Ib beta, V, IX GPs Ib alpha, Ib beta, V, IX OGER GPs Human platelet glycoprotein (GP) V (M(r) 83,300), whose primary structure is reported here, is a part of the Ib-V-IX system of surface glycoproteins (GPs Ib alpha, Ib beta, V, IX) that constitute the receptor for von Willebrand factor (vWf) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. 7690959 1 73 gly glycoprotein 163:174 arg1 GP GP Cterm (GP) V Human platelet glycoprotein (GP) V (M(r) 83,300), whose primary structure is reported here, is a part of the Ib-V-IX system of surface glycoproteins (GPs Ib alpha, Ib beta, V, IX) that constitute the receptor for von Willebrand factor (vWf) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. 2361483 8 59 gly lutropin 1301:1308 arg1 oligosaccharides lutropin oligosaccharides OGER lutropin In addition, our data suggest, for the first time, that N-glycosylation of the receptor may be necessary for expressing functional receptors on the cell surface and that there exist striking similarities in roles of oligosaccharides of lutropin and its receptor. 2521687 1 16 gly glycoprotein 255:266 arg1 Bovine trophoblast protein-1 Bovine trophoblast protein-1 PUBTATOR Bovine trophoblast protein-1 317698 Bovine trophoblast protein-1 (bTP-1) is a secreted glycoprotein that consists of several forms differing slightly in mol wt and isoelectric point. 3759977 2 42 gly glycosylation 107:119 arg1 albumin albumin OGER albumin P02768 Nonenzymatic glycosylation of albumin in vivo occurs at multiple sites. 2886334 1 35 gly glycosylation 277:289 arg1 the cell surface glycoprotein Thy-1 the cell surface glycoprotein Thy-1 PUBTATOR Thy-1 24832 To examine the extent to which protein structure and tissue-type influence glycosylation, we have determined the oligosaccharide structures at each of the three glycosylation sites (Asn-23, 74 and 98) of the cell surface glycoprotein Thy-1 isolated from rat brain and thymus. 7755594 6 86 gly IFN-gamma 993:1001 arg1 The glycan residues IFN-gamma The glycan residues PUBTATOR IFN-gamma 3458 The glycan residues of IFN-gamma, especially at Asn-25, play an important role in protease resistance. 20173767 12 52 gly heterogeneity 1815:1827 arg1 normal human seminal plasma FN normal human seminal plasma FN PUBTATOR FN 2335 Resolving the molecular heterogeneity of normal human seminal plasma FN and gaining initial insight into possible similarities/differences with known FN molecular species may be considered a prerequisite step preceding challenging the clinical usefulness of these molecular properties. 1385812 1 45 gly glycoprotein 201:212 arg1 Granulocyte-macrophage colony-stimulating factor Granulocyte-macrophage colony-stimulating factor PUBTATOR Granulocyte-macrophage colony-stimulating factor 12981 Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a glycoprotein required for the proliferation and differentiation of granulocyte and macrophage precursors. 12090474 3 56 gly O-glycosylated 604:617 arg1 MUC1 MUC1 PUBTATOR MUC1 4582 MUC1 expressed by normal cells has heavily O-glycosylated tandem repeat domain while MUC1 on malignant cells is aberrantly O-glycosylated. 12090474 3 87 gly has 512:514 arg1 MUC1 AND heavily O-glycosylated tandem repeat domain MUC1 heavily O-glycosylated tandem repeat domain PUBTATOR MUC1 4582 MUC1 expressed by normal cells has heavily O-glycosylated tandem repeat domain while MUC1 on malignant cells is aberrantly O-glycosylated. 7780197 6 35 part_of found 886:890 arg2 the TfR AND Asn-727 the TfR Asn-727 PUBTATOR SpecificSite TfR 7037 Asn-727 Glycosylation of Asn-727 found in the TfR purified from human placentae was analysed by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) and mass spectrometry following tryptic digestion, peptide purification via reverse-phase high-performance liquid chromatography (RP-HPLC) and peptide sequencing. 28378791 2 45 gly glycosylation 193:205 arg1 NMDARs NMDARs Cterm NMDARs The effects of glycosylation on the structure and dynamics of NMDARs are largely unknown. 15342690 2 16 gly glycosylated 469:480 arg1 EL EL PUBTATOR EL 9388 Reduction in molecular mass of EL after treatment with glycosidases and after treatment of EL-expressing cells with the glycosylation inhibitor tunicamycin demonstrated that EL is a glycosylated protein. 19955571 10 13 gly modified 1387:1394 arg3 Natural pTF AND sialylated sugars Natural pTF sialylated sugars OGER pTF Natural pTF contains no high mannose glycans but is modified with hybrid, highly fucosylated, and sialylated sugars. 19955571 10 55 gly contains 1347:1354 arg1 Natural pTF AND no high mannose glycans Natural pTF no high mannose glycans OGER pTF Natural pTF contains no high mannose glycans but is modified with hybrid, highly fucosylated, and sialylated sugars. 7680921 1 64 gly glycoprotein 120:131 arg1 CD68 CD68 PUBTATOR CD68 968 CD68 is a 110-Kd transmembrane glycoprotein of unknown function highly expressed by human monocytes and tissue macrophages. 9399579 5 104 gly SDS-PAGE 682:689 arg1 smaller SDS smaller OGER SDS Mouse LGP85 (M-LGP85) from liver lysosomal membranes exhibited an Mr of 80,000 on SDS-PAGE, which is smaller by 5,000 than that of rat LGP85 (R-LGP85). 26555091 5 9 gly beta-2-glycoprotein 1188:1206 arg1 beta-2-glycoprotein 1 beta-2-glycoprotein 1 PUBTATOR beta-2-glycoprotein 1 350 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 9 gly beta-2-glycoprotein 1188:1206 arg1 alpha-1B-glycoprotein alpha-1B-glycoprotein PUBTATOR alpha-1B-glycoprotein 1 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 28 gly zinc-alpha-2-glycoprotein 1371:1395 arg1 zinc-alpha-2-glycoprotein zinc-alpha-2-glycoprotein OGER zinc-alpha-2-glycoprotein P25311 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 apolipoprotein F apolipoprotein F PUBTATOR apolipoprotein F 319 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 alpha-2-HS-glycoprotein alpha-2-HS-glycoprotein OGER alpha-2-HS-glycoprotein P02765 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 fibrinogen fibrinogen PUBTATOR fibrinogen 2244 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 apolipoprotein B-100 apolipoprotein B-100 PUBTATOR apolipoprotein B-100 338 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 alpha-2-macroglobulin alpha-2-macroglobulin PUBTATOR alpha-2-macroglobulin 2 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 beta-2-glycoprotein 1 beta-2-glycoprotein 1 PUBTATOR beta-2-glycoprotein 1 350 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 apolipoprotein D apolipoprotein D PUBTATOR apolipoprotein D 347 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 alpha-1B-glycoprotein alpha-1B-glycoprotein PUBTATOR alpha-1B-glycoprotein 1 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 antithrombin-III antithrombin-III PUBTATOR antithrombin-III 462 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 36 gly alpha-1B-glycoprotein 1041:1061 arg1 ceruloplasmin ceruloplasmin PUBTATOR ceruloplasmin 1356 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 zinc-alpha-2-glycoprotein zinc-alpha-2-glycoprotein OGER zinc-alpha-2-glycoprotein P25311 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 kininogen-1 kininogen-1 OGER kininogen-1 P01042 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 hemopexin hemopexin PUBTATOR hemopexin 3263 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin PUBTATOR immunoglobulin (Ig) A 973 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 serotransferrin serotransferrin PUBTATOR serotransferrin 7018 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 IgG IgG Cterm IgG Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 IgM IgM OGER IgM P01871 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 45 gly glycoproteins 979:991 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 zinc-alpha-2-glycoprotein zinc-alpha-2-glycoprotein OGER zinc-alpha-2-glycoprotein P25311 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 kininogen-1 kininogen-1 OGER kininogen-1 P01042 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 hemopexin hemopexin PUBTATOR hemopexin 3263 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin PUBTATOR immunoglobulin (Ig) A 973 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 serotransferrin serotransferrin PUBTATOR serotransferrin 7018 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 IgG IgG Cterm IgG Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 IgM IgM OGER IgM P01871 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 74 gly N-glycosylation 943:957 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 77 gly alpha-2-HS-glycoprotein 1064:1086 arg1 alpha-2-HS-glycoprotein alpha-2-HS-glycoprotein OGER alpha-2-HS-glycoprotein P02765 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 26555091 5 77 gly alpha-2-HS-glycoprotein 1064:1086 arg1 alpha-1B-glycoprotein alpha-1B-glycoprotein PUBTATOR alpha-1B-glycoprotein 1 Thus, as the assessment of protein glycosylation is becoming a major element in clinical and biopharmaceutical research, this review aims to convey the current state of knowledge on the N-glycosylation of the major plasma glycoproteins alpha-1-acid glycoprotein, alpha-1-antitrypsin, alpha-1B-glycoprotein, alpha-2-HS-glycoprotein, alpha-2-macroglobulin, antithrombin-III, apolipoprotein B-100, apolipoprotein D, apolipoprotein F, beta-2-glycoprotein 1, ceruloplasmin, fibrinogen, immunoglobulin (Ig) A, IgG, IgM, haptoglobin, hemopexin, histidine-rich glycoprotein, kininogen-1, serotransferrin, vitronectin, and zinc-alpha-2-glycoprotein. 24074568 1 42 gly glycans 251:257 arg1 gp120 gp120 glycans PUBTATOR gp120 155971 We examined the ability of HIV-1 subtype C to develop resistance to the inhibitory lectins, griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN), which bind multiple mannose-rich glycans on gp120. 18214858 4 60 gly haptoglobin 886:896 arg1 tri- haptoglobin tri- PUBTATOR haptoglobin 3240 Mass spectrometry analyses demonstrated that concentrations of total fucosylated di-, tri- and tetra-branched glycans of haptoglobin increased in the sera of PC patients. 18214858 4 60 gly haptoglobin 886:896 arg1 tetra-branched glycans haptoglobin tetra-branched glycans PUBTATOR haptoglobin 3240 Mass spectrometry analyses demonstrated that concentrations of total fucosylated di-, tri- and tetra-branched glycans of haptoglobin increased in the sera of PC patients. 18214858 4 60 gly haptoglobin 886:896 arg1 total fucosylated di- haptoglobin total fucosylated di- PUBTATOR haptoglobin 3240 Mass spectrometry analyses demonstrated that concentrations of total fucosylated di-, tri- and tetra-branched glycans of haptoglobin increased in the sera of PC patients. 27681177 7 20 gly deglycosylation 1550:1564 arg1 SLC26A6 SLC26A6 PUBTATOR SLC26A6 65010 Functional studies of transfected SLC26A6 demonstrated that glycosylation at two sites in the putative second extracellular loop of SLC26A6 is critically important for chloride-dependent oxalate transport and that enzymatic deglycosylation of SLC26A6 expressed on the plasma membrane of intact cells strongly reduced oxalate transport activity. 8647124 11 74 gly glycosylated 1974:1985 arg1 the CCKB receptor the CCKB receptor PUBTATOR CCKB receptor 887 Enzymatic deglycosylation of the CCKB receptor with N-glycosidase F after photoaffinity labeling demonstrated that the CCKB receptor with three potential glycosylation sites was slightly glycosylated, amounting to a molecular mass of about 4 kDa. 8647124 11 98 gly deglycosylation 1797:1811 arg1 the CCKB receptor the CCKB receptor PUBTATOR CCKB receptor 887 Enzymatic deglycosylation of the CCKB receptor with N-glycosidase F after photoaffinity labeling demonstrated that the CCKB receptor with three potential glycosylation sites was slightly glycosylated, amounting to a molecular mass of about 4 kDa. 2846759 1 68 gly glycoprotein 157:168 arg1 the glycoprotein H (gH) gene the glycoprotein H (gH) gene PUBTATOR glycoprotein H 1682472 We present the nucleotide sequence of the glycoprotein H (gH) gene of herpesvirus saimiri (HVS), a representative of the T lymphotropic herpesviruses of New World monkeys, and compare the predicted amino acid sequence with sequences of homologous proteins from four human herpesviruses. 7524670 14 70 gly beta 1900:1903 arg1 the sialylated/sulfated fraction eLH beta the sialylated/sulfated fraction Cterm eLH beta Oligosaccharides from the sialylated/sulfated fraction of eLH beta contained both Gal and GalNAc residues at nonreducing termini, and those GalNAc residues were preferentially distributed to the Man alpha 1-->3 side of the trimannosyl core. 28955860 1 35 gly glycoprotein 144:155 arg1 GASP-2 GASP-2 PUBTATOR GASP-2 215001 BACKGROUND: GASP-2 is a secreted multi-domain glycoprotein known as a specific inhibitor of myostatin and GDF-11. 21570947 3 25 gly glycosylated 581:592 arg1 the hSMVT protein the hSMVT protein PUBTATOR hSMVT protein 8884 Our results showed that the hSMVT protein is glycosylated and that this glycosylation is important for its function. 28378791 5 33 gly glycan 590:595 arg1 GluN2B-N688 GluN2B glycan PUBTATOR GluN2B 2904 The glycan on GluN2B-N688 shows a similar, though weaker, effect. 24338886 5 93 part_of position 1013:1020 arg1 Pichia-produced rhEPO Pichia-produced rhEPO position OGER AminoAcid Pichia-produced rhEPO P29676 position Ser126 A low level of O-linked mannosylation was detected on Pichia-produced rhEPO at position Ser126, which is also the O-linked glycosylation site for endogenous human EPO and CHO-derived rhEPO. 7749412 9 8 gly MLD 1443:1445 arg1 all the carriers MLD all the carriers OGER MLD O15121 These observations confirm the genealogical data which pointed to a common ancestor for all the carriers of MLD among the Habbanite Jews. 10559353 1 89 gly glycoprotein 210:221 arg1 gp120 gp120 PUBTATOR gp120 155971 Binding of the extracellular subunit of human immunodeficiency type 1 (HIV-1) envelope (Env) glycoprotein (gp120) to CD4 triggers the induction or exposure of a highly conserved coreceptor binding site in gp120 that helps mediate membrane fusion. 25000122 10 44 gly OPN 1448:1450 arg1 O-glycans OPN O-glycans PUBTATOR OPN 6696 This suggested that some of the phosphorylation in ΔO-OPN caused by O-glycan defects and O-glycans of OPN suppressed the OPN cell-adhesion activity. 25000122 10 44 gly OPN 1448:1450 arg1 O-glycan defects OPN O-glycan defects PUBTATOR OPN 6696 This suggested that some of the phosphorylation in ΔO-OPN caused by O-glycan defects and O-glycans of OPN suppressed the OPN cell-adhesion activity. 10378660 7 65 gly defect 925:930 arg1 mesangial IgA1 IgA1 defect PUBTATOR IgA1 3493 A concomitant O-glycan defect in mesangial IgA1 has not yet been proven. 20615996 8 5 gly O-glycans 1157:1165 arg1 the MUC2 mucin MUC2 mucin O-glycans PUBTATOR MUC2 mucin 4583 The numerous O-glycans on the MUC2 mucin not only serve as nutrients for the bacteria but also as attachment sites and, as such, probably contribute to the selection of the species-specific colon flora. 17803675 2 6 gly O-glycosylation 245:259 arg1 alpha-DG alpha-DG Cterm alpha-DG 13138 Defective O-glycosylation of alpha-dystroglycan (alpha-DG) severs this link leading to muscular dystrophies named dystroglycanopathies. 8307000 4 107 part_of YN-tPA 1366:1371 arg1 Asn117 tPA Asn117 OGER AminoAcid tPA P00750 Asn117 The results revealed that Asn117 of YN-tPA carried exclusively high-mannose-type glycans with five to nine mannose residues similar to wild-type tPA expressed in this cell line [Pfeiffer, G., Schmidt, M., Strube, K.-H. 28280963 12 0 gly glycosylated 1867:1878 arg1 EPO protein EPO protein PUBTATOR EPO protein 2056 N-glycan units were constructed; moreover, EPO protein was glycosylated at potential glycosylation amino acid residue sites. 21535396 0 85 gly Glycosylation 0:12 arg1 tissue factor tissue factor PUBTATOR tissue factor 2152 Glycosylation of tissue factor is not essential for its transport or functions. 16823988 11 11 gly glycosylation 1503:1515 arg1 alpha1-antitrypsin alpha1-antitrypsin OGER alpha1-antitrypsin P01009 Changes in glycosylation sites in cancer serum are also observed by glycopeptide mapping using microLC-ESI-TOF-MS where the N83 glycosylation of alpha1-antitrypsin is down regulated. 10889209 0 43 gly Glycosylation 0:12 arg1 GIRK1 GIRK1 PUBTATOR GIRK1 3760 Glycosylation of GIRK1 at Asn119 and ROMK1 at Asn117 has different consequences in potassium channel function. 11835525 12 11 gly content 1519:1525 arg1 lambda-IgA1 IgA1 content PUBTATOR IgA1 3493 The alpha(2,6)-linked sialic acid content in lambda-IgA1 (300-825 kDa) and kappa-IgA1 (150-610 kDa) from patients was also higher than that of controls. 27726058 10 21 gly glycosylation 1274:1286 arg1 vimentin vimentin PUBTATOR vimentin 81818 However, after three potential glycosylation sites (Ser-7, Thr-33, Ser-34:) of vimentin were mutated to alanine, overexpression of the mutated vimentin completely lost the enhancement activity for the neural differentiation even in the presence of CycloManN pro. 20660194 0 58 gly glycosylation 17:29 arg1 the 5-HT2A receptor the 5-HT2A receptor OGER 5-HT2A receptor P28223 Role of N-linked glycosylation of the 5-HT2A receptor in JC virus infection. 21765645 5 23 gly glycosylation 784:796 arg1 the human serpin alpha-1 antitrypsin the human serpin alpha-1 antitrypsin OGER alpha-1 antitrypsin P01009 We have used optical spectroscopy and hydrogen/deuterium exchange and mass spectrometry to investigate the effects of glycosylation on the human serpin alpha-1 antitrypsin (α(1)-AT). 8992988 9 31 gly glycosylation 1370:1382 arg1 human IgA1 human IgA1 PUBTATOR IgA1 3493 These studies demonstrate that N-linked glycosylation in the constant domain of human IgA1 plays an important role in the biologic properties of IgA1. 28827841 13 44 gly glycoproteins 2076:2088 arg1 the SRR glycoproteins the SRR glycoproteins OGER SRR glycoproteins Q9GZT4 This requirement for the coupling of modification and export may explain the co-evolution of the SRR glycoproteins with their specialized glycan modifying and export systems. 15262264 1 7 gly glycoprotein 173:184 arg1 PrPC PrPC PUBTATOR PrPC 19122 Prion diseases result from conversion of PrPC, a neuronal membrane glycoprotein of unknown function, into PrPSc, an abnormal conformer that is thought to be infectious. 8737716 1 99 gly glycoprotein 230:241 arg1 Serum alpha 1-antitrypsin Serum alpha 1-antitrypsin PUBTATOR Serum alpha 1-antitrypsin 5265 OBJECTIVE: Serum alpha 1-antitrypsin (alpha 1AT) is an acute-phase glycoprotein which contains three carbohydrate side chains, N-glycosidically linked to the asparagine molecules (Asn46, Asn83 and Asn247) of the single polypeptide unit. 8737716 1 35 gly contains 249:256 arg1 Serum alpha 1-antitrypsin AND three carbohydrate side chains Serum alpha 1-antitrypsin three carbohydrate side chains PUBTATOR Serum alpha 1-antitrypsin 5265 OBJECTIVE: Serum alpha 1-antitrypsin (alpha 1AT) is an acute-phase glycoprotein which contains three carbohydrate side chains, N-glycosidically linked to the asparagine molecules (Asn46, Asn83 and Asn247) of the single polypeptide unit. 16834341 3 33 gly glycopeptides 370:382 arg1 human pituitary follicle stimulating hormone human pituitary follicle stimulating hormone Cterm follicle stimulating hormone In the present study the negatively charged glycopeptides of equine and human pituitary follicle stimulating hormone (eFSH and hFSH) have been characterized in a glycosylation site-specific manner using FT-ICR-MS and Edman sequencing. 2536708 7 7 gly deglycosylated 1135:1148 arg1 deglycosylated beta subunit deglycosylated beta subunit OGER subunit P0DN86 Dimers containing deglycosylated beta subunit and an alpha subunit lacking either the Asn-52 oligosaccharide or both oligosaccharides fail to stimulate cAMP or steroid formation. 15545280 3 4 gly glycan 509:514 arg1 ICAM-2 ICAM-2 glycan PUBTATOR ICAM-2 3384 The first N-linked glycan in ICAM-2 contacts an exposed tryptophan residue, defining a conserved glycan-W motif critical for the conformation of the integrin binding domain. 6651835 0 18 gly glycosylation 11:23 arg1 mouse immunoglobulin M. Mouse immunoglobulin IgM mouse immunoglobulin M. Mouse immunoglobulin IgM OGER IgM P01872 Incomplete glycosylation of Asn 563 in mouse immunoglobulin M. Mouse immunoglobulin IgM was prepared from MOPC 104E ascites fluid and [3H]-mannose labeled tumor cells. 2498325 9 15 gly N-glycosylated 1435:1448 arg1 this apoE this apoE PUBTATOR apoE 348 Studies with tunicamycin indicated that this apoE was N-glycosylated at Asn194. 1413513 7 14 gly glycoproteins 1758:1770 arg1 the human and bovine SH glycoproteins the human and bovine SH glycoproteins PUBTATOR SH glycoproteins 8431 To identify conserved amino acid residues among the human and bovine SH glycoproteins that may function as signals for polylactosaminoglycan modification, the nucleotide sequences of the SH protein genes of a human subgroup B virus (8/60) and a bovine virus (391-2) were determined and compared to those of a human subgroup A virus (A2), a subgroup B virus (18537), and a bovine virus (A51908). 29760280 4 52 gly glycosylated 413:424 arg1 complex glycosylated CD133 complex glycosylated CD133 PUBTATOR CD133 8842 Ubiquitination occurs primarily on complex glycosylated CD133. 26947874 2 50 gly N-glycosylation 264:278 arg1 A1AT A1AT PUBTATOR A1AT 5265 However, the effects of an additional N-glycosylation in the unstructured region or the loop region of alpha-1 antitrypsin (A1AT) on the circulatory half-life of the protein are largely unknown. 21380457 2 16 gly haptoglobin 247:257 arg1 glycan changes haptoglobin glycan changes PUBTATOR haptoglobin 3240 Many studies have reported glycan changes of haptoglobin in diseases such as breast cancer and pancreatic cancer. 1453482 1 20 gly glycoprotein 113:124 arg1 MOG MOG PUBTATOR MOG 24558 Myelin/oligodendrocyte glycoprotein (MOG) is a primary target autoantigen in experimental autoimmune encephalomyelitis, a widely used animal model for autoimmune demyelinating diseases such as multiple sclerosis. 1453482 1 20 gly glycoprotein 113:124 arg1 Myelin/oligodendrocyte glycoprotein Myelin/oligodendrocyte glycoprotein PUBTATOR Myelin/oligodendrocyte glycoprotein 24558 Myelin/oligodendrocyte glycoprotein (MOG) is a primary target autoantigen in experimental autoimmune encephalomyelitis, a widely used animal model for autoimmune demyelinating diseases such as multiple sclerosis. 30209313 1 22 gly glycoprotein 176:187 arg1 Env Env PUBTATOR Env 155971 As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans. 30209313 1 22 gly glycoprotein 176:187 arg1 the envelope glycoprotein the envelope glycoprotein PUBTATOR envelope glycoprotein 155971 As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans. 12050356 2 24 gly glycoprotein 383:394 arg1 Env Env Cterm Env The envelope glycoprotein (Env) encoded by HERV-W is highly fusogenic, is naturally expressed in human placental syncytiatrophoblasts, and has been reported to function as a superantigen in lymphocyte cultures. 26807597 2 73 gly modified 254:261 arg1 Human RNA polymerase II AND O-linked N-acetylglucosamine Human RNA polymerase II O-linked N-acetylglucosamine OGER RNA polymerase II Human RNA polymerase II (Pol II) is extensively modified by O-linked N-acetylglucosamine (O-GlcNAc) on its unique C-terminal domain (CTD), which consists of 52 heptad repeats. 26807597 2 73 gly modified 254:261 arg3 Human RNA polymerase II AND O-GlcNAc Human RNA polymerase II O-GlcNAc OGER RNA polymerase II Human RNA polymerase II (Pol II) is extensively modified by O-linked N-acetylglucosamine (O-GlcNAc) on its unique C-terminal domain (CTD), which consists of 52 heptad repeats. 26327323 5 64 gly N-glycosylated 691:704 arg1 wild type TEVp wild type TEVp Cterm TEVp While wild type TEVp targeted to the secretory pathway of mammalian cells is synthetized as an N-glycosylated and catalytically inactive enzyme, a TEVp mutant with selected mutations at two verified N-glycosylation sites and at an exposed cysteine was highly efficient. 30030822 5 19 gly Notch 807:811 arg1 the EGF repeats Notch the EGF repeats Cterm Notch Three types of O-linked glycosylation occur at consensus sequences found within the EGF repeats of Notch: O-fucosylation, O-glucosylation, and O-GlcNAcylation. 30030822 5 19 gly Notch 807:811 arg1 O-fucosylation Notch O-fucosylation Cterm Notch Three types of O-linked glycosylation occur at consensus sequences found within the EGF repeats of Notch: O-fucosylation, O-glucosylation, and O-GlcNAcylation. 19918835 6 25 gly Mdm2 788:791 arg1 the most important determinant Mdm2 protein, a the most important determinant PUBTATOR Mdm2 protein, a 4193 The Mdm2 protein, a negative regulator of p53, is the most important determinant of p53 abundance and subcellular localization. 8757998 1 44 gly glycoprotein 150:161 arg1 human cytomegalovirus (HCMV) glycoprotein B human cytomegalovirus (HCMV) glycoprotein B Cterm human cytomegalovirus (HCMV) glycoprotein B (gB Intracellular processing of human cytomegalovirus (HCMV) glycoprotein B (gB; gpUL55) expressed by a recombinant adenovirus (Ad-gB) was studied in human A549 cells as processing events could affect immunogenicity when such viruses are used as live-recombinant vaccines. 18642129 5 14 gly O-glycosylation 692:706 arg1 pd-FVII pd-FVII OGER FVII P08709 N- and O-glycosylation sites and site specific heterogeneity of pd-FVII were studied by various complementary qualitative and quantitative techniques. 18642129 5 84 gly heterogeneity 732:744 arg1 pd-FVII pd-FVII OGER FVII P08709 N- and O-glycosylation sites and site specific heterogeneity of pd-FVII were studied by various complementary qualitative and quantitative techniques. 1549584 3 10 gly glycosylation 576:588 arg1 gp120 gp120 PUBTATOR gp120 3700 In this study, we evaluated the relative importance of each of the 24 N-linked glycosylation sites of gp120 in the molecular clone HXB2 to viral infectivity. 8496193 4 2 gly glycosylation 625:637 arg1 procathepsin L trafficking procathepsin L OGER procathepsin L P06797 To study the role of glycosylation in procathepsin L trafficking, we constructed a cDNA in which the codon for Asn-204 was mutated to encode Gln. 20174636 7 82 part_of Env 1293:1295 arg1 Env V1-V5 region Env Env V1-V5 region PUBTATOR SiteSequence Env 100616444 V1-V5 region Furthermore, the newly transmitted viruses from the infant had significantly fewer potential N-linked glycosylation sites in Env V1-V5 region and showed a propensity to encode shorter variable loops compared to the nontransmitted viruses. 25617829 0 15 gly N-glycosylation 0:14 arg1 GPRC6A receptor expression GPRC6A receptor PUBTATOR GPRC6A receptor 222545 N-glycosylation and disulfide bonding affects GPRC6A receptor expression, function, and dimerization. 7664637 1 92 gly glycosylation 187:199 arg1 Tg Tg glycosylation Cterm Tg 24826 Lack of completion of N-acetyllactosamine-type glycosylation on thyroglobulin (Tg) has been implicitly considered as an etiological factor of some thyroid disorders, i.e. goiter and hypothyroidism. 7664637 1 92 gly glycosylation 187:199 arg1 thyroglobulin thyroglobulin glycosylation PUBTATOR thyroglobulin 24826 Lack of completion of N-acetyllactosamine-type glycosylation on thyroglobulin (Tg) has been implicitly considered as an etiological factor of some thyroid disorders, i.e. goiter and hypothyroidism. 3169232 0 1 gly site 17:20 arg1 mutant antithrombin antithrombin site PUBTATOR antithrombin 462 New carbohydrate site in mutant antithrombin (7 Ile----Asn) with decreased heparin affinity. 17675499 1 53 gly glycoforms 125:134 arg1 human MUC1 human MUC1 PUBTATOR MUC1 4582 The targeting of epitopes on tumor-associated glycoforms of human MUC1 represents a primary goal in immunotherapeutic anticancer strategies. 26150355 0 19 gly glycoprotein 79:90 arg1 matrix metalloproteinase-9 glycoprotein secretion matrix metalloproteinase-9 glycoprotein secretion PUBTATOR matrix metalloproteinase-9 glycoprotein 4318 LMAN1 (ERGIC-53) is a potential carrier protein for matrix metalloproteinase-9 glycoprotein secretion. 15482257 4 28 gly glycosylated 651:662 arg1 their large glycosylated ECD their large glycosylated ECD OGER ECD O95905 They differ at the very N-terminus of their large glycosylated ECD (extracellular domain). 8916420 2 59 gly glycoprotein 226:237 arg1 Interferon-gamma Interferon-gamma PUBTATOR Interferon-gamma 3458 Interferon-gamma (IFN-gamma) is a dimeric, secretory glycoprotein produced by T-lymphocytes. 3200844 3 43 gly glycoprotein 402:413 arg1 np62 np62 PUBTATOR np62 65274 The major (62 kDa) nuclear pore glycoprotein (np62) was purified from rat liver nuclear envelopes by immunoaffinity chromatography and preparative gel electrophoresis. 10682309 0 45 gly non-glycosylated 2:17 arg1 SAP-B SAP-B OGER SAP O60880 A non-glycosylated and functionally deficient mutant (N215H) of the sphingolipid activator protein B (SAP-B) in a novel case of metachromatic leukodystrophy (MLD). 28474680 5 17 gly YAP 860:862 arg1 O-GlcNAcylation YAP O-GlcNAcylation PUBTATOR YAP 10413 Importantly, we found via in vitro cell-based and in vivo mouse model experiments that O-GlcNAcylation of YAP was required for high-glucose-induced liver tumorigenesis. 17050611 0 5 gly Pradimicin 0:9 arg1 a carbohydrate-binding nonpeptidic lead compound Pradimicin A a carbohydrate-binding nonpeptidic lead compound Cterm Pradimicin A Pradimicin A, a carbohydrate-binding nonpeptidic lead compound for treatment of infections with viruses with highly glycosylated envelopes, such as human immunodeficiency virus. 11069996 3 62 gly moiety 613:618 arg1 gp120 gp120 moiety PUBTATOR gp120 3700 Our data suggest that this carbohydrate moiety on gp120 blocks access to the binding site for CD4 and modulates the chemokine receptor binding site of phenotypically diverse clade A and clade B isolates. 16779786 2 34 gly glycoprotein 532:543 arg1 AGP AGP Cterm AGP AGP is a glycoprotein known to display disease specific changes in glycosylation and although this secondary modification is not directly involved in drug binding, it may influence the conformation of the binding site. 30158294 14 21 gly glycosylated 2296:2307 arg1 SERINC5 SERINC5 PUBTATOR SERINC5 256987 Here we show that SERINC5 is a glycosylated protein and that N-glycosylation is important for its steady state expression. 14691230 3 0 gly glycosylation 628:640 arg1 DPPIV DPPIV PUBTATOR DPPIV 1803 Crystallographic studies on DPPIV reveal clear N-linked glycosylation of nine Asn residues in DPPIV. 22279061 12 38 gly SREC-I 1794:1799 arg1 Asn(289)-, Asn(382)- and Asn(393)-linked N-glycans SREC-I Asn(289)-, Asn(382)- and Asn(393)-linked N-glycans OGER SREC-I Q14162 These data indicate that Asn(289)-, Asn(382)- and Asn(393)-linked N-glycans of SREC-I have distinct functions in regulating proteolytic resistance, ligand-binding affinity and subcellular localization, all of which might be involved in the development of atherogenesis. 2536098 5 34 gly glycosylation 1344:1356 arg1 gp120 gp120 PUBTATOR gp120 3700 However, production of the glycoprotein in the presence of tunicamycin and removal of the N-linked sugars by endoglycosidase H treatment both resulted in deglycosylated proteins that were unable to bind to CD4, suggesting in agreement with previous results, that glycosylation contributes to the ability of gp120 to bind to CD4. 19171054 6 48 gly hyperglycosylated 1023:1039 arg1 the variant hyperglycosylated hCG the variant hyperglycosylated hCG OGER hCG While regular hCG is made by fused villous syncytiotrophoblast cells, extravillous invasive cytotrophoblast cells make the variant hyperglycosylated hCG. 9610390 6 6 gly receptor 953:960 arg1 the carbohydrate chains ETA receptor the carbohydrate chains PUBTATOR ETA receptor 24326 We suggest that in both cerebellar and atrial membranes, the carbohydrate chains of the ETA receptor contribute to the binding of ligand to the nanomolar-affinity binding sites, but not to the picomolar-affinity binding sites. 18340083 6 22 gly glycan 846:851 arg1 GPIHBP1 GPIHBP1 glycan PUBTATOR GPIHBP1 68453 The glycan was marginally sensitive to endoglycosidase F2 digestion but resistant to endoglycosidase F3 digestion, suggesting that the glycan on GPIHBP1 is of the oligomannose type. 12505154 2 34 part_of has 210:212 arg1 MCHR1 AND Asn16 MCHR1 Asn13, Asn16 and Asn23 PUBTATOR AminoAcid MCHR1 83567 Asn13, Asn16 and Asn23 MCHR1 has three potential sites (Asn13, Asn16 and Asn23) for N-linked glycosylation in its extracellular amino-terminus which may modulate its reactivity. 12505154 2 34 part_of has 210:212 arg1 MCHR1 AND Asn13 MCHR1 Asn13, Asn16 and Asn23 PUBTATOR AminoAcid MCHR1 83567 Asn13, Asn16 and Asn23 MCHR1 has three potential sites (Asn13, Asn16 and Asn23) for N-linked glycosylation in its extracellular amino-terminus which may modulate its reactivity. 12505154 2 34 part_of has 210:212 arg1 MCHR1 AND Asn13 MCHR1 Asn13, Asn16 and Asn23 PUBTATOR AminoAcid MCHR1 83567 Asn13, Asn16 and Asn23 MCHR1 has three potential sites (Asn13, Asn16 and Asn23) for N-linked glycosylation in its extracellular amino-terminus which may modulate its reactivity. 7629496 6 60 gly glycosylation 1028:1040 arg1 5E6 protein 5E6 protein PUBTATOR 5E6 protein 16634 The sequence of 5E6 mRNA and the degree of glycosylation of 5E6 protein are under genetic control. 16103099 2 54 gly glycosylated 420:431 arg1 glycosylated RECK glycosylated RECK PUBTATOR RECK 8434 The predicted amino acid sequence of human RECK includes five putative N-glycosylation sites; however, the precise biochemical role of glycosylated RECK remains unknown. 11563913 4 58 gly BSSL 806:809 arg1 alpha-helix BSSL alpha-helix PUBTATOR BSSL 1056 This variant lacks the C-terminal alpha-helix and tandem C-terminal repeat region of native BSSL, but retains full catalytic activity. 28733331 4 5 gly N-glycosylation 416:430 arg1 FNDC5 FNDC5 PUBTATOR FNDC5 252995 In the present study, we analysed N-glycosylation sites of FNDC5 and found that two potential N-glycosylation sites (Asn36 and Asn81) could indeed be occupied by N-glycan. 8091655 4 75 gly glycoprotein 458:469 arg1 HSV-1 glycoprotein K HSV-1 glycoprotein K Cterm HSV-1 glycoprotein K ORF53 (MDV gK), a homolog to HSV-1 glycoprotein K (gK), is 1062 nucleotides long and encodes 354 amino acids (39.5 kDa). 2380335 2 78 gly glycosylation 469:481 arg1 G-hPRL G-hPRL PUBTATOR hPRL 5617 Variations in the glycosylation pattern of G-hPRL altered its receptor-binding properties, suggesting that the site of glycosylation may be proximal to the receptor-binding region. 2082620 2 45 gly N-glycosylation 353:367 arg1 HIV-1 gp120 HIV-1 gp120 PUBTATOR gp120 155971 To address the possible role of N-glycosylation of HIV-1 gp120 in binding CD4, we mutated different conserved N-glycosylation site Asn-residues in the vicinity of the putative CD4 binding site, as single mutations or in combinations. 9020858 3 6 gly O-glycosylated 510:523 arg1 LAP LAP PUBTATOR LAP 7939 All TNAPs proved to be N-glycosylated, and only the liver isoform (LAP) is not O-glycosylated. 24884609 10 23 gly O-glycoforms 1413:1424 arg1 isoform-specific ITIH4 O-glycoforms isoform-specific ITIH4 O-glycoforms PUBTATOR ITIH4 3700 We also identified isoform-specific ITIH4 O-glycoforms and documented that utilization of O-glycosylation sites on ITIH4 differed between the cell line and serum. 14711516 6 3 gly Recombinant 1003:1013 arg1 GlcNAc Recombinant Thy-1 GlcNAc PUBTATOR Recombinant Thy-1 7070 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 3 gly Recombinant 1003:1013 arg1 2 Recombinant Thy-1 2 PUBTATOR Recombinant Thy-1 7070 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 3 gly Recombinant 1003:1013 arg1 Man Recombinant Thy-1 Man PUBTATOR Recombinant Thy-1 7070 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 3 gly Recombinant 1003:1013 arg1 5 Recombinant Thy-1 5 PUBTATOR Recombinant Thy-1 7070 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 41 gly oligosaccharides 1097:1112 arg1 asparagine 23 and 100 asparagine 23 and 100 asparagine 23 and 100 SpecificSite asparagine 23 and 100 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 14711516 6 41 gly oligosaccharides 1097:1112 arg1 100 100 100 SpecificSite asparagine 23 and 100 Recombinant Thy-1 from Lec1 cells contained (GlcNAc)(2)(Man)(5) on asparagine 60, whereas the oligosaccharides on asparagine 23 and 100 contained approximately 80% (GlcNAc)(2)(Man)(4) and approximately 20% (GlcNAc)(2)(Man)(5). 1733926 1 1 gly glycoprotein 133:144 arg1 Colony stimulating factor-1 Colony stimulating factor-1 PUBTATOR Colony stimulating factor-1 12977 Colony stimulating factor-1 (CSF-1) is a homodimeric glycoprotein that humorally regulates the proliferation and differentiation of mononuclear phagocytic cells and locally regulates cells of the female reproductive tract. 11520040 5 34 gly glycoforms 786:795 arg1 the hTf glycoforms the hTf glycoforms OGER hTf P02787 Furthermore, we demonstrate using a combination of online immunoaffinity-postconcentration-mass spectrometry in conjunction with a blood spot cartridge that we can determine the relative quantities of the hTf glycoforms using <5 microL blood in under 30 min. 9184148 1 70 gly chain 257:261 arg1 Asn-135 Asn-135 Asn-135 SpecificSite Asn-135 The beta-form of antithrombin, lacking a carbohydrate side chain on Asn-135, is known to bind heparin more tightly than the fully glycosylated alpha-form. 8981095 5 38 gly transferrin 996:1006 arg1 The oligosaccharide population transferrin The oligosaccharide population OGER transferrin P02787 The oligosaccharide population of human serum transferrin was analysed by a series of mixed exoglycosidase digests on the released glycan pool and quantified using a novel HPLC strategy. 21637607 1 50 gly glycosylated 138:149 arg1 MUC1 MUC1 PUBTATOR MUC1 4582 MUC1 is a heavily glycosylated mammalian transmembrane protein expressed by mucosal secretory tissues for both protection against microbial infection and lubrication. 2477227 3 29 gly beta 384:387 arg1 the carbohydrate composition hCG beta the carbohydrate composition PUBTATOR hCG beta 1082 In the present study we used recently developed technology to determine the carbohydrate composition of beta-core and hCG beta (CR119). 17609437 4 109 gly glycosylated 728:739 arg1 large free GPHalpha large free GPHalpha PUBTATOR GPHalpha 1081 In JEG-3 cells the early 22-kDa GPHalpha either associated with hCGbeta, or showed self-association to yield GPHalpha alpha homodimers, or was later converted into heavily glycosylated large free GPHalpha (M(r app) = 24 kDa). 7964612 12 26 gly glycosylation 1766:1778 arg1 M protein secretion M protein OGER M protein P54296 However, glycosylation was not essential for M protein secretion since M protein deprived of glycosylation by tunicamycin treatment was detected in the medium. 27427791 6 23 gly O-deglycosylation 792:808 arg1 oFN oFN Cterm oFN 2335 In line with this, adding oFN that underwent enzymatic O-deglycosylation to osteoblasts normalized nodule formation in vitro. 3457370 9 55 gly contains 1594:1601 arg1 ovalbumin AND oligosaccharides ovalbumin oligosaccharides PUBTATOR ovalbumin 396058 In addition to high-mannose and hybrid oligosaccharide chains, ovalbumin synthesized in L cells contains oligosaccharides of the complex type. 15322230 7 3 gly P-glycoprotein 1391:1404 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Enhanced ubiquitination of P-glycoprotein resulted in a decrease of the function of the transporter, as demonstrated by increased intracellular drug accumulation and increased cellular sensitivity to drugs transported by P-glycoprotein. 15322230 7 23 gly P-glycoprotein 1197:1210 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 Enhanced ubiquitination of P-glycoprotein resulted in a decrease of the function of the transporter, as demonstrated by increased intracellular drug accumulation and increased cellular sensitivity to drugs transported by P-glycoprotein. 22442073 0 50 gly N-glycosylation 0:14 arg1 acid-sensing ion channel 1a acid-sensing ion channel 1a PUBTATOR acid-sensing ion channel 1a 11419 N-glycosylation of acid-sensing ion channel 1a regulates its trafficking and acidosis-induced spine remodeling. 11425798 1 13 gly N-glycosylation 82:96 arg1 NCAM NCAM PUBTATOR NCAM 17967 The N-glycosylation pattern of the neural cell adhesion molecule (NCAM), isolated from brains of newborn mice, has been analyzed. 11425798 1 13 gly N-glycosylation 82:96 arg1 the neural cell adhesion molecule the neural cell adhesion molecule PUBTATOR neural cell adhesion molecule 17967 The N-glycosylation pattern of the neural cell adhesion molecule (NCAM), isolated from brains of newborn mice, has been analyzed. 8486654 1 63 gly glycosylated 118:129 arg1 Macrosialin Macrosialin PUBTATOR Macrosialin 12514 Macrosialin is a heavily glycosylated transmembrane protein of 87-115 kDa, highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. 24361716 3 51 gly glycosylated 628:639 arg1 ADAM17 ADAM17 PUBTATOR ADAM17 6868 We found that ADAM17 expressed in mammalian cells was more heavily glycosylated than its insect-expressed analog. 9719680 13 96 gly present 1941:1947 arg1 SPACR AND The carbohydrate chains SPACR The carbohydrate chains PUBTATOR SPACR 3617 The carbohydrate chains present on SPACR could also provide sites for extensive crosslinking and participate in the formation of the ordered IPM lattice that surrounds the elongate photoreceptors projecting from the outer retinal surface. 8442916 3 88 gly glycoprotein 531:542 arg1 gp120 gp120 PUBTATOR gp120 3700 The molecular analysis of the env region encompassed all variable domains of the external glycoprotein, gp120. 26402790 2 58 gly glycoprotein 280:291 arg1 Physiologically active FSH Physiologically active FSH OGER FSH Physiologically active FSH is a glycoprotein that can accommodate glycans on up to four asparagine residues, including two sites in the FSHα subunit that are critical for biochemical function, plus two sites in the β subunit, whose differential glycosylation states appear to correspond to physiologically distinct functions. 12399462 5 46 gly modifications 1084:1096 arg1 MuSK AND N-linked carbohydrate modifications MuSK N-linked carbohydrate modifications OGER MuSK O15146 We identifed two N-linked glycosylation sites in MuSK, and we expressed MuSK mutants lacking one or both N-linked sites into MuSK mutant myotubes to determine whether N-linked carbohydrate modifications of MuSK have a role in MuSK activation. 12399462 5 49 gly MuSK 1101:1104 arg1 N-linked carbohydrate modifications MuSK N-linked carbohydrate modifications OGER MuSK O15146 We identifed two N-linked glycosylation sites in MuSK, and we expressed MuSK mutants lacking one or both N-linked sites into MuSK mutant myotubes to determine whether N-linked carbohydrate modifications of MuSK have a role in MuSK activation. 20022931 8 72 gly N-glycosylated 1857:1870 arg1 MC2R MC2R PUBTATOR MC2R 4158 Taken together, these results indicate that the absence of MC2R N-glycosylation abrogates to a large extent MC2R cell surface expression in the absence of MRAPs, whereas when MC2R is N-glycosylated, it can be expressed at the plasma membrane without MRAP assistance. 28880909 5 73 gly glycosylation 1050:1062 arg1 FSHR FSHR PUBTATOR FSHR 2492 The focus of this analysis was the site-specific glycosylation at asparagine (Asn) 52 of the α-subunit of FSH, owing to the pivotal role of Asn52 glycosylation in FSH receptor (FSHR) activation/signalling. 28880909 5 73 gly glycosylation 1050:1062 arg1 FSH receptor FSH receptor PUBTATOR FSH receptor 2492 The focus of this analysis was the site-specific glycosylation at asparagine (Asn) 52 of the α-subunit of FSH, owing to the pivotal role of Asn52 glycosylation in FSH receptor (FSHR) activation/signalling. 1482372 10 57 gly oligosaccharide 1153:1167 arg1 beta APP APP oligosaccharide OGER APP P05067 In CHO cells the N-linked oligosaccharide on beta APP was completely resistant to Endoglycosidase H, suggesting that it is of complex type. 17498123 9 8 gly O-glycosylated 1799:1812 arg1 aberrantly O-glycosylated IgA1 aberrantly O-glycosylated IgA1 PUBTATOR IgA1 3493 This review will discuss the evidence for the role of IgA1 O-glycosylation in the pathogenesis of IgAN and propose an explanation for the presence of aberrantly O-glycosylated IgA1 in the circulation of patients with IgAN. 17498123 9 55 gly O-glycosylation 1697:1711 arg1 IgAN IgAN PUBTATOR IgAN 60498 This review will discuss the evidence for the role of IgA1 O-glycosylation in the pathogenesis of IgAN and propose an explanation for the presence of aberrantly O-glycosylated IgA1 in the circulation of patients with IgAN. 15505805 0 53 gly glycoprotein 25:36 arg1 ADAM23 ADAM23 PUBTATOR ADAM23 301460 ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons. 11418591 1 16 gly molecule 184:191 arg1 Poly-alpha-2,8-sialic acid neural cell adhesion molecule Poly-alpha-2,8-sialic acid PUBTATOR neural cell adhesion molecule 4684 Poly-alpha-2,8-sialic acid (polysialic acid) is a post-translational modification of the neural cell adhesion molecule (NCAM) and an important regulator of neuronal cell-cell interactions. 8416385 6 21 gly chains 980:985 arg1 gp120 gp120 chains PUBTATOR gp120 155971 An experiment which measured the ability of gp120 to bind to CD4 as an assay of the proper conformation of gp120 showed that carbohydrate chains on gp120 are not required for the interaction between gp120 and CD4 but that N-linked glycosylation is essential for generation of the proper conformation of gp120 to provide a CD4-binding site. 27480168 0 51 gly glycosylation 9:21 arg1 equilibrative nucleoside transporter 1 equilibrative nucleoside transporter 1 PUBTATOR equilibrative nucleoside transporter 1 2030 N-linked glycosylation of N48 is required for equilibrative nucleoside transporter 1 (ENT1) function. 27480168 0 51 gly glycosylation 9:21 arg1 ENT1 ENT1 PUBTATOR ENT1 2030 N-linked glycosylation of N48 is required for equilibrative nucleoside transporter 1 (ENT1) function. 10861210 2 70 gly contained 502:510 arg1 Human AE1 AND a high-mannose oligosaccharide Human AE1 a high-mannose oligosaccharide PUBTATOR Human AE1 6521 Human AE1 expressed in transfected human embryonic kidney (HEK)-293 or COS-7 cells contained a high-mannose oligosaccharide. 26563299 3 32 gly glycosylation 531:543 arg1 hetIL-15 hetIL-15 PUBTATOR IL-15 3600 Favorable pharmacokinetic properties are associated with the heterodimeric formation and the glycosylation of hetIL-15, which, however, remains largely uncharacterized. 18274893 0 6 gly N-glycosylated 178:191 arg1 biologically active N-glycosylated 15N-labeled phCG biologically active N-glycosylated 15N-labeled phCG Cterm phCG High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains--selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG. 19584017 1 15 gly glycoproteins 231:243 arg1 the envelope glycoproteins the envelope glycoproteins PUBTATOR envelope glycoproteins 17276 Three N-linked glycosylation sites were removed from the envelope glycoproteins of Friend, Moloney, and AKV mouse ecotropic gammaretroviruses: gs1 and gs2, in the receptor binding domain; and gs8, in a region implicated in post-binding cell fusion. 19584017 1 15 gly glycoproteins 231:243 arg1 gs2 gs2 PUBTATOR gs2 8228 Three N-linked glycosylation sites were removed from the envelope glycoproteins of Friend, Moloney, and AKV mouse ecotropic gammaretroviruses: gs1 and gs2, in the receptor binding domain; and gs8, in a region implicated in post-binding cell fusion. 19584017 1 15 gly glycoproteins 231:243 arg1 gs1 gs1 PUBTATOR gs1 8226 Three N-linked glycosylation sites were removed from the envelope glycoproteins of Friend, Moloney, and AKV mouse ecotropic gammaretroviruses: gs1 and gs2, in the receptor binding domain; and gs8, in a region implicated in post-binding cell fusion. 3048385 1 1 gly glycoprotein 183:194 arg1 Sulfated glycoprotein 1 Sulfated glycoprotein 1 PUBTATOR Sulfated glycoprotein 1 25524 Sulfated glycoprotein 1 (SGP-1) is one of the abundant proteins secreted by rat Sertoli cells. 3048385 1 1 gly glycoprotein 183:194 arg1 SGP-1 SGP-1 PUBTATOR SGP-1 25524 Sulfated glycoprotein 1 (SGP-1) is one of the abundant proteins secreted by rat Sertoli cells. 28596490 6 37 gly glycosylation 963:975 arg1 GPER GPER PUBTATOR GPER 2852 We discovered that interfering with N-linked glycosylation of GPER, either by mutation of the predicted glycosylation sites or pharmacologically with tunicamycin, drives GPER into the nucleus. 25971727 1 54 gly glycosylated 145:156 arg1 Epidermal growth factor receptor Epidermal growth factor receptor PUBTATOR Epidermal growth factor receptor 1956 Epidermal growth factor receptor (EGFR) is a heavily glycosylated transmembrane receptor tyrosine kinase. 2963825 6 59 gly non-glycosylated 993:1008 arg1 non-glycosylated proliferin non-glycosylated proliferin PUBTATOR proliferin 18811 Furthermore, non-glycosylated proliferin did not inhibit the binding of the glycosylated protein. 2246236 13 77 gly glycosylation 1549:1561 arg1 functional vEGF receptors functional vEGF receptors PUBTATOR vEGF receptors 281572 The expression of functional vEGF receptors was inhibited when the cells were preincubated with tunicamycin, indicating that glycosylation of the receptor is important for the expression of functional vEGF receptors. 21056893 4 6 gly mutations 369:377 arg1 GP1 GP1 mutations PUBTATOR GP1 9567 N-linked glycan mutations at positions 87 and 97 on GP1 resulted in reduction of expression and absence of cleavage and were necessary for downstream functions, as confirmed by the loss of GP-mediated fusion activity with T87A and S97A mutants. 12888867 0 45 gly glycosylation 26:38 arg1 plasminogen activator inhibitor-1 plasminogen activator inhibitor-1 PUBTATOR plasminogen activator inhibitor-1 5054 Biochemical importance of glycosylation of plasminogen activator inhibitor-1. 21795704 4 27 gly glycosylation 666:678 arg1 T T Cterm T 43 Here, several lines of evidence indicate that the N-linked glycosylation of AChE(T) plays a major role for acquisition of AChE full enzymatic activity but does not affect its oligomerization. 21795704 4 27 gly glycosylation 666:678 arg1 AChE AChE PUBTATOR AChE 43 Here, several lines of evidence indicate that the N-linked glycosylation of AChE(T) plays a major role for acquisition of AChE full enzymatic activity but does not affect its oligomerization. 26420485 11 39 gly glycosylation 1477:1489 arg1 RDS RDS PUBTATOR RDS 19133 These data suggest that glycosylation of RDS is required for RDS function or stability in cones, a difference that may be due to extracellular versus intradiscal localization of the RDS glycan in cones versus rods. 15628971 6 51 gly attached 1280:1287 arg1 C4ST-1 AND N-linked oligosaccharides C4ST-1 N-linked oligosaccharides PUBTATOR C4ST-1 314694 These observations strongly suggest that N-linked oligosaccharides attached to C4ST-1 contribute to the production and stability of the active form of C4ST-1. 1381541 8 14 gly carbohydrate 1274:1285 arg1 E1 E1 carbohydrate Cterm E1 Our findings suggest that although carbohydrate on E1 is not directly involved in the antigenic structures of E1, it is important in maintaining proper protein folding and stable conformation for expression of immunological epitopes on E1. 18815274 9 9 gly glycosylated 1250:1261 arg1 SV2A SV2A PUBTATOR SV2A 64051 Together, the data reported here demonstrate that glycosylated SV2A and SV2B act in conjunction with gangliosides to mediate the entry of BoNT/E into neurons. 20127679 5 5 gly glycosylation 769:781 arg1 CD44 CD44 PUBTATOR CD44 960 By deletion of the carbohydrate-recognition domain region and mutation of crucial amino acids involved in carbohydrate-recognition of LSECtin and by inhibition of the N-linked glycosylation of CD44, we further demonstrated that the interaction between CD44 and LSECtin is dependent on protein-glycan recognition. 20127679 5 50 gly LSECtin 727:733 arg1 carbohydrate-recognition LSECtin carbohydrate-recognition PUBTATOR LSECtin 339390 By deletion of the carbohydrate-recognition domain region and mutation of crucial amino acids involved in carbohydrate-recognition of LSECtin and by inhibition of the N-linked glycosylation of CD44, we further demonstrated that the interaction between CD44 and LSECtin is dependent on protein-glycan recognition. 30115684 1 29 gly glycan 124:129 arg1 the envelope glycoprotein gp120 gp120 glycan PUBTATOR gp120 155971 The glycan shield on the envelope glycoprotein gp120 of human immunodeficiency virus 1 (HIV-1) has drawn immense attention as a vulnerable site for broadly neutralizing antibodies (bNAbs) and for its significant impact on host adaptive immune response to HIV-1. 8639654 11 107 gly N-glycosylation 2147:2161 arg1 the human VIP 1 receptor the human VIP 1 receptor PUBTATOR VIP 1 receptor 7432 These studies highlight the functional importance of the N-glycosylation of the human VIP 1 receptor which belongs to a new subfamily of seven membrane-spanning receptors. 1847926 2 18 gly N-glycosylation 152:166 arg1 MPR 46 MPR 46 PUBTATOR MPR 46 4074 Using site-directed mutagenesis the N-glycosylation sites of the Mr 46,000 mannose 6-phosphate receptor (MPR 46) were identified as asparagine residues 57, 83, 107, and 113. 24780636 5 84 gly sTf 845:847 arg1 the N-glycans sTf the N-glycans Cterm sTf 7018 Mass spectrometric analyses confirmed that Tf-2 is modified with disialylated biantennary glycans at both of the two N-glycosylation sites, which are similar to the N-glycans of sTf. 25707740 18 23 gly glycoforms 2053:2062 arg1 hCG hCG OGER hCG Depending on its source of production, glycoforms of hCG display different biological activities and functions that are essential for pregnancy outcome. 24269691 3 39 gly KIR2DS5 324:330 arg1 these alleles KIR2DS5 these alleles PUBTATOR KIR2DS5 3810 The proteins encoded by these alleles (KIR2DS5*002-*009) are expressed at varying levels on the surface of NKL and Jurkat transfectants. 13679364 4 21 gly polysialylated 561:574 arg1 polysialylated NCAM polysialylated NCAM PUBTATOR NCAM 4684 Polysialic acid is primarily attached to N-glycans of NCAM, and polysialylated NCAM is expressed on the outer surface of myotube bundles. 13679364 4 69 gly NCAM 551:554 arg1 N-glycans NCAM N-glycans PUBTATOR NCAM 4684 Polysialic acid is primarily attached to N-glycans of NCAM, and polysialylated NCAM is expressed on the outer surface of myotube bundles. 10413465 6 23 part_of apoE4 1298:1302 arg1 position 158 apoE4 position 158 PUBTATOR SpecificSite apoE4 348 cysteine at position 158 In contrast, introduction of cysteine at position 158 of apoE4 (Arg112, Cys158) increased the SDS-stable binding of apoE to Abeta to the levels similar to those observed in apoE2. 26105115 3 10 gly density 372:378 arg1 the gp120 subunit gp120 subunit density PUBTATOR gp120 subunit 3700 The unusually high glycan density on the gp120 subunit limits processing during biosynthesis, leaving a region of under-processed oligomannose-type structures, which is a primary target of these bnAbs. 11595658 0 38 gly N-glycosylation 0:14 arg1 CRF receptor type 1 CRF receptor type 1 PUBTATOR CRF receptor type 1 1394 N-glycosylation of CRF receptor type 1 is important for its ligand-specific interaction. 20042514 6 33 gly nonglycosylated 1206:1220 arg1 a nonglycosylated H protein a nonglycosylated H protein OGER H protein Q13203 To further characterize the role of N glycosylation in CDV pathogenesis, the N-glycosylation sites of wild-type H proteins were successively deleted, including a nonstandard site, to ultimately generate a nonglycosylated H protein. 27681177 8 4 gly deglycosylation 1828:1842 arg1 SLC26A6 SLC26A6 PUBTATOR SLC26A6 65010 Taken together, these studies indicated that oxalate transport function of SLC26A6 is critically dependent on glycosylation and that exoglycosidase-mediated deglycosylation of SLC26A6 has the capacity to profoundly modulate SLC26A6 function. 29181010 4 26 gly glycoforms 422:431 arg1 Fc glycoforms Fc glycoforms Cterm Fc Fc glycoforms affect mAbs effector functions including antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) by modulating the Fc-FcγRs and Fc-C1q interactions. 8702647 5 12 gly glycosylated 1067:1078 arg1 the CaR the CaR OGER CaR P41180 Seven inactivating mutations, which cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism, show a reduced functional activity of the receptor because they may 1) reduce its affinity for agonists; 2) prevent conversion of the receptor from a putatively immature, high mannose form into the fully glycosylated and biologically active form of the CaR, in addition to lowering its affinity for agonists; or 3) fail to couple the receptor to and/or activate its respective G protein(s). 23959878 6 7 gly deglycosylated 924:937 arg1 native and deglycosylated hIDUA native and deglycosylated hIDUA PUBTATOR hIDUA 3425 The kinetics of native and deglycosylated hIDUA suggested that the N-glycan is also involved in catalytic processes. 26482295 7 60 gly glycosylate 1332:1342 arg1 human immunoglobulin G human immunoglobulin G Cterm human immunoglobulin G One notable example is D. gigas PglB, which was the only bacterial OST to glycosylate the Fc domain of human immunoglobulin G at its native 'QYNST' sequon. 10725420 7 82 gly presence 1387:1394 arg2 gp120/gp41 AND a unique carbohydrate structure gp120/gp41 a unique carbohydrate structure PUBTATOR gp120 3700 Although host cell glycoproteins are incorporated into the membrane of HIV, binding of virus to immobilized MBL required expression of gp120/gp41 on virus particles, suggesting the presence of either an unusually high carbohydrate density and/or a unique carbohydrate structure on gp120/gp41 that is the target of MBL. 10725420 7 82 gly presence 1387:1394 arg2 gp120/gp41 AND an unusually high carbohydrate density gp120/gp41 an unusually high carbohydrate density PUBTATOR gp120 3700 Although host cell glycoproteins are incorporated into the membrane of HIV, binding of virus to immobilized MBL required expression of gp120/gp41 on virus particles, suggesting the presence of either an unusually high carbohydrate density and/or a unique carbohydrate structure on gp120/gp41 that is the target of MBL. 9139799 0 97 gly receptor 66:73 arg1 The six N-linked carbohydrates lutropin/choriogonadotropin receptor The six N-linked carbohydrates PUBTATOR lutropin/choriogonadotropin receptor 3973 The six N-linked carbohydrates of the lutropin/choriogonadotropin receptor are not absolutely required for correct folding, cell surface expression, hormone binding, or signal transduction. 29933399 3 60 gly glycoprotein 303:314 arg1 Env Env PUBTATOR Env 155971 The HIV-1 envelope glycoprotein (Env) is the sole viral target of bnAbs, but is also targeted by binding, non-neutralizing antibodies. 29933399 3 60 gly glycoprotein 303:314 arg1 The HIV-1 envelope glycoprotein The HIV-1 envelope glycoprotein PUBTATOR envelope glycoprotein 155971 The HIV-1 envelope glycoprotein (Env) is the sole viral target of bnAbs, but is also targeted by binding, non-neutralizing antibodies. 19093876 4 40 gly O-glycosylation 805:819 arg1 APP APP OGER APP P05067 Unlike N-glycosylation, no sites of O-glycosylation of APP have previously been reported. 2668275 12 14 gly contain 1566:1572 arg1 STS AND mannose 6-phosphate residues STS mannose 6-phosphate residues Cterm STS 412 In spite of its similarity with these two lysosomal sulfatases, STS does not contain mannose 6-phosphate residues and is transported to lysosomes by a mannose 6-phosphate receptor-independent mechanism. 21752569 2 9 gly attached 384:391 arg2 Human IgA1 AND The glycans Human IgA1 The glycans PUBTATOR Human IgA1 3493 The glycans attached to Human IgA1 were removed from their attachment sites by an array of enzymes. 9166287 2 26 gly glycosylated 301:312 arg1 immunoreactive glycodelin immunoreactive glycodelin PUBTATOR glycodelin 5047 Recently, we found that seminal plasma contains immunoreactive glycodelin (GdS) that is differentially glycosylated and has no contraceptive activity. 9166287 2 26 gly glycosylated 301:312 arg1 GdS GdS Cterm GdS 5047 Recently, we found that seminal plasma contains immunoreactive glycodelin (GdS) that is differentially glycosylated and has no contraceptive activity. 9184148 0 32 gly chain 25:29 arg1 Asn-135 Asn-135 Asn-135 SpecificSite Asn-135 The oligosaccharide side chain on Asn-135 of alpha-antithrombin, absent in beta-antithrombin, decreases the heparin affinity of the inhibitor by affecting the heparin-induced conformational change. 19549906 7 27 gly E-cadherin 1041:1050 arg1 the complex N-glycans E-cadherin the complex N-glycans OGER E-cadherin P12830 Partial inhibition of DPAGT1 with small interfering RNA reduced the complex N-glycans of E-cadherin and increased the abundance of alpha-catenin and stabilizing proteins in adherens junctions. 28827841 11 57 gly glycoproteins 1785:1797 arg1 SRR glycoproteins SRR glycoproteins OGER SRR glycoproteins Q9GZT4 These results demonstrate that Asp2 is a bifunctional protein involved in both the post-translational modification and transport of SRR glycoproteins. 26084674 0 40 gly β-glycoform 20:30 arg1 Activated protein C β-glycoform protein C OGER protein C P02810 Activated protein C β-glycoform promotes enhanced noncanonical PAR1 proteolysis and superior resistance to ischemic injury. 12527108 0 44 gly N-glycosylation 0:14 arg1 recombinant human fucosyltransferase III recombinant human fucosyltransferase III PUBTATOR fucosyltransferase III 2525 N-glycosylation of recombinant human fucosyltransferase III is required for its in vivo folding in mammalian and insect cells. 24327294 4 59 gly glycosylated 720:731 arg1 glycosylated BChE glycosylated BChE PUBTATOR BChE 590 However, the asparagine-linked glycans on the surface of glycosylated BChE may interfere with the PEGylation modification. 21561106 9 73 gly glycosylation 1679:1691 arg1 hFcγRIIIa hFcγRIIIa PUBTATOR hFcγRIIIa 2214 Using surface plasmon resonance (SPR) interaction analysis, we show that the cell type and site specific glycosylation pattern of hFcγRIIIa influences its binding behavior to immunoglobulin molecules. 7538124 0 75 gly glycosylation 46:58 arg1 human keratin 18 human keratin 18 PUBTATOR keratin 18 3875 Identification and mutational analysis of the glycosylation sites of human keratin 18. 8349827 2 95 gly glycoprotein 418:429 arg1 The GlyCAM 1 glycoprotein The GlyCAM 1 glycoprotein PUBTATOR GlyCAM 1 glycoprotein 14663 The GlyCAM 1 glycoprotein has been previously shown to be expressed specifically by the endothelial cells of peripheral and mesenteric lymph nodes and in an unknown site in lung. 25567004 3 33 gly hyper-glycosylated 752:769 arg1 BSG BSG PUBTATOR BSG 12215 Recombinant low-glycosylated secreted BHc products (BSK) were also immunologically inert, similar to hyper-glycosylated BHc products (BSG), although deglycosylation restored their immunological activities. 25567004 3 50 gly low-glycosylated 663:678 arg1 BSK BSK PUBTATOR BSK 450219 Recombinant low-glycosylated secreted BHc products (BSK) were also immunologically inert, similar to hyper-glycosylated BHc products (BSG), although deglycosylation restored their immunological activities. 9720213 0 41 gly glycosylation 18:30 arg1 the rat leukemia inhibitory factor the rat leukemia inhibitory factor PUBTATOR leukemia inhibitory factor 60584 Asparagine-linked glycosylation of the rat leukemia inhibitory factor expressed by simian COS7 cells. 10089210 0 44 gly N-Glycosylation 0:14 arg1 a mouse IgG a mouse IgG Cterm IgG N-Glycosylation of a mouse IgG expressed in transgenic tobacco plants. 29036200 2 11 gly glycosylation 384:396 arg1 the DG α subunit the DG α subunit OGER subunit Q14118 A crucial role in the glycosylation of the DG α subunit is played by its own N-terminal region that is required by the glycosyltransferase LARGE. 24211831 3 28 gly O-glycans 465:473 arg1 CD45 CD45 O-glycans PUBTATOR CD45 5788 In this study, no apoptosis induced by galectin-3 was detected in CD45RO-transfected cells, whereas apoptosis of CD45RABC-transfected cells was observed, implying that O-glycans on CD45 might play roles in galectin-3-induced apoptosis. 9790679 0 13 gly contains 14:21 arg1 Human DNase I AND mannose 6-phosphate Human DNase I mannose 6-phosphate OGER DNase I P24855 Human DNase I contains mannose 6-phosphate and binds the cation-independent mannose 6-phosphate receptor. 8388021 8 26 gly glycoprotein 1280:1291 arg1 the envelope glycoprotein the envelope glycoprotein PUBTATOR envelope glycoprotein 17276 The results demonstrate the importance of critical sites within the envelope glycoprotein as determinants of virus virulence. 11467948 3 15 gly glycoprotein 625:636 arg1 TNFR-IgG TNFR-IgG PUBTATOR TNFR 7132 To increase the serum half-life of these glycoproteins, we carried out in vitro glycosylation experiments using TNFR-IgG, an immunoadhesin molecule, as a model therapeutic glycoprotein. 3655744 1 59 gly glycoproteins 173:185 arg1 E1 E1 Cterm E1 The complete nucleotide sequence of the genes coding for the two membrane glycoproteins E1 and E2 of rubella virus has been determined from cloned cDNA derived from the 40S genomic RNA. 7711052 7 51 gly presence 1555:1562 arg2 tissue plasminogen activator AND an N-linked glycan tissue plasminogen activator the kringle 2 domain an N-linked glycan OGER tissue plasminogen activator P00750 domain The presence of an N-linked glycan (at Asn-184) in the kringle 2 domain of tissue plasminogen activator hinders the rearrangement of this ternary complex, decreasing the turnover rate (Kcat). 6980014 6 52 gly contain 902:908 arg1 II AND one carbohydrate chain II fragments II and III each one carbohydrate chain Cterm Site II fragments On the other hand, fragments II and III each contain one carbohydrate chain exclusively by the biantennary type of complex N-glycoside. 7576532 3 89 part_of contains 558:565 arg1 IL-Mu6 AND Glu100<==>Asn IL-Mu6 Glu100<==>Asn Cterm AminoAcid IL-Mu6 Glu100 IL-Mu6 contains a single amino acid substitution (Glu100<==>Asn) generating a potential N-glycosylation recognition site (Asn100-Xxx-Thr/Ser) in addition to the natural O-glycosylation at position Thr3. 29415129 1 19 gly glycosylated 86:97 arg1 Heavily glycosylated secreted mucin MUC5AC Heavily glycosylated secreted mucin MUC5AC PUBTATOR MUC5AC 17833 Heavily glycosylated secreted mucin MUC5AC, by the virtue of its cysteine-rich repeats, can form inter- and intramolecular disulfide linkages resulting in complex polymers, which in turn craft the framework of the polymeric mucus gel on epithelial cell surfaces. 20622017 0 46 gly glycosylation 26:38 arg1 renal and hepatic γ-glutamyl transpeptidase renal and hepatic γ-glutamyl transpeptidase PUBTATOR -glutamyl transpeptidase 102724197 Analysis of site-specific glycosylation of renal and hepatic γ-glutamyl transpeptidase from normal human tissue. 8096511 9 34 gly P-glycoprotein 1401:1414 arg1 a constrained P-glycoprotein structure a constrained P-glycoprotein structure PUBTATOR P-glycoprotein 5243 This, and possibly the observed lack of glycosylation of the remaining intact glycosylation sequence, suggests a constrained P-glycoprotein structure. 27095603 6 43 gly glycopeptides 1108:1120 arg1 native transferrin native transferrin PUBTATOR transferrin 7018 In this technical note, the aberrant glycosylation profiles of CDG cases are presented to shed light on the MS of native transferrin and glycopeptides from the viewpoint of clinical glycoproteomics. 14764083 1 6 gly glycoprotein 150:161 arg1 Lactoferrin Lactoferrin PUBTATOR Lactoferrin 280846 Lactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. 16103099 4 12 gly glycosylated 582:593 arg1 RECK protein RECK protein PUBTATOR RECK protein 8434 RECK protein was glycosylated at Asn86, Asn200, Asn297, and Asn352 residues but not at the Asn39 residue in HT1080 cells. 23014585 10 15 gly glycosylation 1491:1503 arg1 P-selectin P-selectin PUBTATOR P-selectin 6403 We conclude that the 715Pro variant impairs terminal glycosylation of P-selectin in Golgi, leading to reduced amounts of mature P-selectin and subsequently less surface expression and secretion of P-selectin. 1318404 1 20 gly glycoprotein 184:195 arg1 SU (gp70) SU (gp70) Cterm SU The role of the N-linked glycosylation sites in the major envelope glycoprotein, SU (gp70), of Moloney murine leukemia virus has been examined. 20805301 5 12 gly glycosylation 689:701 arg1 CYP2W1 CYP2W1 PUBTATOR CYP2W1 54905 Bioinformatic analysis identified Asn177 as the only possible glycosylation site of CYP2W1, which was supported by the inability of an N177A mutant to be glycosylated in HEK 293 cells. 18814249 4 69 gly glycoproteins 664:676 arg1 gp21 gp21 Cterm gp21 Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. 26088564 0 66 gly glycoprotein 41:52 arg1 human α1-acid glycoprotein human α1-acid glycoprotein Cterm α1-acid Structural glycobiology of human α1-acid glycoprotein and its implications for pharmacokinetics and inflammation. 8323299 7 1 part_of lamp-1 1218:1223 arg1 residues 167 to 190 lamp-1 residues 167 to 190 PUBTATOR SpecificSite lamp-1 3916 residues 167 Circular dichroism and nuclear magnetic resonance spectroscopy was used for the structural characterization of a synthetic peptide corresponding to residues 167 to 190 of lamp-1. 25673720 14 6 gly glycosylated 2165:2176 arg1 C C Cterm C Q61171 We infected mice that express different forms of glycosylated PrP(C) with three different TSE agents. 25673720 14 6 gly glycosylated 2165:2176 arg1 glycosylated PrP glycosylated PrP OGER PrP Q61171 We infected mice that express different forms of glycosylated PrP(C) with three different TSE agents. 9578495 5 11 gly choriogonadotropin 693:710 arg1 the alpha52 oligosaccharide choriogonadotropin the alpha52 oligosaccharide OGER choriogonadotropin Therefore, we conclude that the alpha52 oligosaccharide of choriogonadotropin is not involved in signal transduction, but in the stability of the heterodimer. 30111774 5 34 gly O-glycosylated 778:791 arg1 MUC1 MUC1 OGER MUC1 P15941 Moreover, immunoblotting assays demonstrated reactivity to tumour-associated O-glycosylated proteins, such as MUC1. 11447837 5 89 gly glycosylation 773:785 arg1 APP APP OGER APP P05067 An alteration in the glycosylation state of APP by the generation of oligomannosyl oligosaccharides results in a decrease in the secretion of the neuroprotective, soluble form of the protein and a parallel increase in the deposition of the cellular protein within the perinuclear region of the cell. 8798614 1 68 gly glycoprotein 176:187 arg1 CD59 CD59 PUBTATOR CD59 966 CD59 is a glycosylphosphatidylinositol-anchored membrane glycoprotein that serves as the principle cellular inhibitor of the C5b-9 membrane attack complex (MAC) of human complement. 22828516 2 24 gly Glycosylation 284:296 arg1 Apa Apa OGER Apa Q07075 Glycosylation of Apa plays a key role in colonization and invasion of the host cells by M. tuberculosis through interactions of Apa with the host immune system C-type lectins. 21570947 5 53 part_of hSMVT 964:968 arg1 the putative PKC phosphorylation site hSMVT the putative PKC phosphorylation site PUBTATOR SpecificSite hSMVT 8884 site Thr(286) Mutating the putative PKC phosphorylation site Thr(286) of hSMVT led to a significant decrease in the PMA-induced inhibition in biotin uptake. 21570947 5 57 part_of PKC 927:929 arg1 the putative PKC phosphorylation site PKC the putative PKC phosphorylation site PUBTATOR SpecificSite PKC 112476 site Thr(286) Mutating the putative PKC phosphorylation site Thr(286) of hSMVT led to a significant decrease in the PMA-induced inhibition in biotin uptake. 10462522 7 42 gly glycoprotein 1322:1333 arg1 p67 p67 OGER p67 P61764 Colabelling with an antibody against p67, a lysosomal glycoprotein of trypanosomes, revealed extensive overlap between the proteins with opposing relative abundance. 8609432 1 10 gly deglycosylated 177:190 arg1 deglycosylated released Fc gamma RIIIa deglycosylated released Fc gamma RIIIa PUBTATOR Fc gamma RIIIa 2214 A donor-dependent difference in electrophoretic mobility of deglycosylated released Fc gamma RIIIa derived from NK cells and macrophages was observed. 3811284 1 19 gly Glycosylation 57:69 arg1 human blood serum albumin human blood serum albumin PUBTATOR serum albumin 213 Glycosylation of human blood serum albumin was carried out by means of prolonged incubation of the protein with an excess of D-glucose or D-glucose-6-phosphate. 8973534 4 66 gly glycoprotein 959:970 arg1 glycoprotein I glycoprotein I Cterm glycoprotein I The genes encoding MDV2 protein kinase (PK), gD, and glycoprotein I (gI) homologues are transcribed to form 3' coterminal mRNAs of 6.0 kb (encoding PK, gD and gI) and 4.2 kb (encoding gD and gI), respectively. 8663239 7 94 gly glycosylation 1410:1422 arg1 de novo synthesized IGF-1R proteins de novo synthesized IGF-1R proteins PUBTATOR IGF-1R proteins 3480 The N-linked glycosylation and the expression of de novo synthesized IGF-1R proteins at the cell surface as well as the number of IGF-1 binding sites were completely restored upon replenishment of MVA. 1637954 10 59 gly deglycosylated 1646:1659 arg1 deglycosylated hCG deglycosylated hCG OGER hCG In contrast, in heavy membranes the affinity for deglycosylated hCG (6.30 +/- 0.19.10(9) M-1), was significantly higher than that for native hCG (2.60 +/- 0.13.10(9) M-1), with no significant differences in receptor number. 1737783 1 23 gly glycoprotein 109:120 arg1 Human CD4 Human CD4 PUBTATOR Human CD4 920 Human CD4, a monomeric T cell surface glycoprotein, is required for T helper cell activation and is also the receptor for the human immunodeficiency virus. 25948743 2 55 gly glycoprotein 297:308 arg1 the viral attachment glycoprotein G the viral attachment glycoprotein G OGER glycoprotein G P07996 These two viruses bind the cellular entry receptors ephrin B2 and/or ephrin B3 via the viral attachment glycoprotein G, and the concerted efforts of G and the viral fusion glycoprotein F result in membrane fusion. 25948743 2 131 gly glycoprotein 365:376 arg1 the viral fusion glycoprotein F the viral fusion glycoprotein F Cterm the viral fusion glycoprotein F These two viruses bind the cellular entry receptors ephrin B2 and/or ephrin B3 via the viral attachment glycoprotein G, and the concerted efforts of G and the viral fusion glycoprotein F result in membrane fusion. 1705556 5 4 gly deglycosylated 860:873 arg1 deglycosylated CD13 deglycosylated CD13 PUBTATOR CD13 290 The epitopes are protein and not carbohydrate as both monoclonals were able to precipitate deglycosylated CD13 from tunicamycin- and monensin-treated cells and o-glycanase-treated purified CD13. 21799112 2 22 gly present 352:358 arg1 HIV-1 gp120 gp120 present PUBTATOR gp120 155971 This antiviral activity is attributed to two homologous carbohydrate binding sites that specifically bind high mannose glycosylation present on envelope glycoproteins such as HIV-1 gp120. 21799112 2 31 gly glycoproteins 372:384 arg1 HIV-1 gp120 HIV-1 gp120 PUBTATOR gp120 155971 This antiviral activity is attributed to two homologous carbohydrate binding sites that specifically bind high mannose glycosylation present on envelope glycoproteins such as HIV-1 gp120. 10364201 0 18 gly glycoprotein 74:85 arg1 the human erythrocyte anion exchanger 1 the human erythrocyte anion exchanger 1 OGER anion exchanger 1 P02730 Calnexin interaction with N-glycosylation mutants of a polytopic membrane glycoprotein, the human erythrocyte anion exchanger 1 (band 3). 3288503 0 9 gly attached 34:41 arg1 human renin AND N-linked oligosaccharides human renin N-linked oligosaccharides PUBTATOR renin 5972 Role of N-linked oligosaccharides attached to human renin expressed in COS cells. 12867999 1 4 gly glycoprotein 184:195 arg1 Clusterin Clusterin PUBTATOR Clusterin 1191 Clusterin is a heterodimeric, disulfide-linked 70-80 kDa glycoprotein that is induced during regression of most, if not all, hormone-dependent epithelial tissues. 29717387 5 48 gly N-glycosylation 614:628 arg1 PPARγ PPARγ OGER PPAR Q07869 Disruption of both sites by site-directed mutagenesis completely abrogated the N-glycosylation of PPARγ. 24361716 5 36 gly glycosylation 983:995 arg1 ADAM17 ADAM17 PUBTATOR ADAM17 6868 The mammalian form of ADAM17 exhibited 10- to 30-fold lower kcat values than the insect analog, while the KM was unaffected, suggesting that glycosylation of ADAM17 can potentially play a role in regulating enzyme activity in vivo. 7835977 0 44 gly glycosylation 8:20 arg1 recombinant human complement component C9 recombinant human complement component C9 PUBTATOR complement component C9 735 Altered glycosylation and selected mutation in recombinant human complement component C9: effects on haemolytic activity. 27833947 1 24 gly glycosylated 103:114 arg1 Erythropoietin Erythropoietin PUBTATOR Erythropoietin 2056 Erythropoietin (Epo) is a heavily glycosylated protein, with its main function being related to erythropoiesis, where it controls red blood cell production via interaction with the Epo receptor (EpoR). 24820161 7 29 gly HYAL1 1021:1025 arg1 C-mannosylation HYAL1 C-mannosylation PUBTATOR HYAL1 3373 Computer simulation demonstrated that C-mannosylation of HYAL1 at Trp¹³⁰ changed conformation of the catalytic active site, and faced Glu¹³¹ in the opposite direction toward its substrate, HA, indicating that C-mannosylation will negatively regulate its secretion, and will attenuate its enzymatic activity. 24820161 7 40 gly C-mannosylation 1002:1016 arg1 HYAL1 HYAL1 PUBTATOR HYAL1 3373 Computer simulation demonstrated that C-mannosylation of HYAL1 at Trp¹³⁰ changed conformation of the catalytic active site, and faced Glu¹³¹ in the opposite direction toward its substrate, HA, indicating that C-mannosylation will negatively regulate its secretion, and will attenuate its enzymatic activity. 21627585 4 72 part_of hPAR 776:779 arg1 Cys hPAR(2) Cys PUBTATOR SpecificSite hPAR(2) 2150 Cys(361) We have demonstrated, using autoradiography, that Cys(361) is the primary palmitoylation site of hPAR(2). 23161435 1 26 gly PTMs 160:163 arg1 the most complex form PTMs the most complex form PUBTATOR PTMs 5763 Glycosylation is the most complex form of protein PTMs. 8702538 8 96 gly glycoprotein 1372:1383 arg1 myelin-associated glycoprotein myelin-associated glycoprotein OGER myelin-associated glycoprotein P20916 Site-directed mutagenesis of similar NC(T/S) motifs in the first or second Ig domains of the I-type lectins myelin-associated glycoprotein, and sialoadhesin did not disrupt their ability to mediate sialic acid binding. 29888865 5 84 gly glycoprotein 590:601 arg1 Background/Objective Human factor VIII Background/Objective Human factor VIII PUBTATOR factor VIII 2157 SUMMARY: Background/Objective Human factor VIII (FVIII) is a plasma glycoprotein, defects of which result in hemophilia A. Current substitution therapy uses FVIII products purified from human plasma or from various cell lines (recombinant FVIII) with different levels of B-domain deletion. 8429003 6 59 part_of Asn159 1280:1285 arg1 rat sCD4 sCD4 Asn159 PUBTATOR AminoAcid sCD4 499358 Asn159 The glycosylation at the conserved site at Asn270 of rat sCD4 was identical to that seen for the equivalent site in human sCD4, and the oligomannose and hybrid structures were restricted to the nonconserved site at Asn159 in rat sCD4. 8429003 6 65 part_of sCD4 1122:1125 arg1 Asn270 sCD4 Asn270 PUBTATOR AminoAcid sCD4 499358 Asn270 The glycosylation at the conserved site at Asn270 of rat sCD4 was identical to that seen for the equivalent site in human sCD4, and the oligomannose and hybrid structures were restricted to the nonconserved site at Asn159 in rat sCD4. 7529232 4 61 gly CD2 562:564 arg1 single N-acetylglucosamine residues CD2 single N-acetylglucosamine residues PUBTATOR CD2 914 A form of human soluble CD2 (hsCD2) with single N-acetylglucosamine residues at each glycosylation site was produced by inhibiting glucosidase I with N-butyldeoxynojirimycin during expression in Chinese hamster ovary cells and digesting the expressed hsCD2 with endoglycosidase H. 23389049 0 46 gly glycoforms 14:23 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Site-specific glycoforms of haptoglobin in liver cirrhosis and hepatocellular carcinoma. 10988251 3 50 gly carries 561:567 arg1 nonmutated alpha1m AND two short non-sialylated N-linked oligosaccharides nonmutated alpha1m two short non-sialylated N-linked oligosaccharides PUBTATOR alpha1m 252922 The biochemical properties of N17,96Q-alpha1m were compared to nonmutated alpha1m, which carries two short non-sialylated N-linked oligosaccharides when expressed in the same system. 3816803 9 41 gly colligin 1673:1680 arg1 N-linked oligosaccharides colligin N-linked oligosaccharides PUBTATOR colligin 12406 99, 1416-1423 (1984)], removal of N-linked oligosaccharides from colligin had no effect on its binding to native type IV collagen. 15841140 0 38 gly glycosylated 32:43 arg1 aberrantly glycosylated serum IgA1 aberrantly glycosylated serum IgA1 PUBTATOR IgA1 3493 [Binding capacity of aberrantly glycosylated serum IgA1 with human umbilical vein endothelial cells]. 28822114 5 39 gly glycosylation 704:716 arg1 DMP1 DMP1 PUBTATOR DMP1 13406 The only glycosylation site of DMP1 is serine89 (S89) in the N-terminal domain of the protein in mouse. 29050309 2 22 gly glycoprotein 275:286 arg1 Plasma fibronectin Plasma fibronectin PUBTATOR Plasma fibronectin 2335 Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. 1859403 9 83 gly glycoprotein 1506:1517 arg1 LGP85 LGP85 PUBTATOR LGP85 117106 LGP85 appears to be an unique lysosomal membrane glycoprotein that does not require tyrosine residues for targeting to lysosomes. 9054441 3 68 gly contain 308:314 arg1 Plasminogen 2 AND only an O-linked oligosaccharide Plasminogen 2 only an O-linked oligosaccharide OGER Plasminogen 2 P00747 Plasminogen 2 is known to contain only an O-linked oligosaccharide at Thr-345. 25541284 1 42 gly glycoprotein 132:143 arg1 MOG MOG PUBTATOR MOG 4340 Myelin oligodendrocyte glycoprotein (MOG) is a type I integral membrane protein that is expressed in the central nervous system. 25541284 1 42 gly glycoprotein 132:143 arg1 Myelin oligodendrocyte glycoprotein Myelin oligodendrocyte glycoprotein PUBTATOR Myelin oligodendrocyte glycoprotein 4340 Myelin oligodendrocyte glycoprotein (MOG) is a type I integral membrane protein that is expressed in the central nervous system. 12742580 1 48 gly glycoprotein 157:168 arg1 VSG VSG Cterm VSG The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. 20566871 4 67 gly glycosylated 660:671 arg1 Uncleaved Rem Uncleaved Rem PUBTATOR Uncleaved Rem 19700 Uncleaved Rem was partially glycosylated, but mutations in both glycosylation sites within the C terminus prevented Rem function. 16219759 2 57 gly glycosylation 300:312 arg1 PrP PrP PUBTATOR PrP 19122 It has been suggested that glycosylation of PrP can influence the susceptibility to transmissible spongiform encephalopathy and determine the characteristics of the many different strains observed in this particular type of disease. 9099948 4 44 gly sialylation 589:599 arg1 mZP3 mZP3 PUBTATOR mZP3 22788 Here, we examined the influence of extent of glycosylation, sulfation, and sialylation of mZP3 (M(r) approximately 65,000-100,000) on its bioactivity; i.e. its ability to inhibit binding of sperm to eggs and to induce the acrosome reaction in vitro. 9099948 4 54 gly mZP3 604:607 arg1 sialylation mZP3 sialylation PUBTATOR mZP3 22788 Here, we examined the influence of extent of glycosylation, sulfation, and sialylation of mZP3 (M(r) approximately 65,000-100,000) on its bioactivity; i.e. its ability to inhibit binding of sperm to eggs and to induce the acrosome reaction in vitro. 9099948 4 82 gly glycosylation 559:571 arg1 mZP3 mZP3 PUBTATOR mZP3 22788 Here, we examined the influence of extent of glycosylation, sulfation, and sialylation of mZP3 (M(r) approximately 65,000-100,000) on its bioactivity; i.e. its ability to inhibit binding of sperm to eggs and to induce the acrosome reaction in vitro. 14970177 5 50 gly glycosylated 1036:1047 arg1 glycosylated hIL-6 glycosylated hIL-6 OGER hIL-6 P05231 As distinct from vIL-6, unglycosylated hIL-6 is as potent as glycosylated hIL-6 in stimulating B cell proliferation. 14970177 5 58 gly unglycosylated 999:1012 arg1 unglycosylated hIL-6 unglycosylated hIL-6 OGER hIL-6 P05231 As distinct from vIL-6, unglycosylated hIL-6 is as potent as glycosylated hIL-6 in stimulating B cell proliferation. 20618438 4 16 part_of protein 603:609 arg1 residues HFE protein residues PUBTATOR SpecificSite HFE protein 3077 residues N110, N130 and N234 Here we employed bioinformatics to identify putative N-glycosylation sites at residues N110, N130 and N234 of the human HFE protein, and used site-directed mutagenesis to create combinations of single, double or triple mutants. 27294781 1 50 gly glycosylated 376:387 arg1 glycosylated human SV2C glycosylated human SV2C PUBTATOR SV2C 22987 Here we report a 2.0-Å-resolution crystal structure of the BoNT/A1 receptor-binding domain in complex with its neuronal receptor, glycosylated human SV2C. 19470663 1 66 gly glycosylation 135:147 arg1 alpha-DG alpha-DG Cterm alpha-DG Q62165 Inactivating mutations of Large reduce the functional glycosylation of alpha-dystroglycan (alpha-DG) and lead to muscular dystrophy in mouse and humans. 21474642 9 46 gly glycosylated 1323:1334 arg1 human glycosylated proBNP human glycosylated proBNP PUBTATOR BNP 4879 The terminal half-life for human glycosylated proBNP was 9.0 (0.5) min compared with 6.4 (0.5) min for BNP. 7918455 12 65 gly r-apo 1915:1919 arg1 the carbohydrate moiety apo(a) the carbohydrate moiety PUBTATOR apo(a) 4018 Extracellular association with LDL was not affected by the carbohydrate moiety of r-apo(a), indicating a protein-protein interaction between r-apo(a) and apoB. 1637954 9 71 gly deglycosylated 1457:1470 arg1 deglycosylated hCG deglycosylated hCG OGER hCG In light membranes there were significantly more receptor sites for deglycosylated hCG (11.2 +/- 4.8 fmol/mg ovary) than for native hCG (4.8 +/- 0.7 fmol/mg ovary), with no significant different in affinity. 7492680 0 92 gly glycoprotein 67:78 arg1 a mouse oviduct-specific glycoprotein a mouse oviduct-specific glycoprotein PUBTATOR oviduct-specific glycoprotein 12659 Molecular cloning and characterization of a mouse oviduct-specific glycoprotein. 23376777 10 97 gly glycosylation 1744:1756 arg1 KCC4 KCC4 PUBTATOR KCC4 20499 We propose that glycosylation is essential for the surface expression, stabilization, and bioactivity of KCC4. 23443134 5 69 gly observed 1049:1056 arg2 the histone acetyltransferase CREB-binding protein AND O-GlcNAc modification the histone acetyltransferase CREB-binding protein O-GlcNAc modification OGER CREB-binding protein Q92793 O-GlcNAc modification was observed on enzymes involved in post-translational regulation, including MAST4 and WNK1 kinases, a ubiquitin-associated protein (UBAP2l), and the histone acetyltransferase CREB-binding protein. 23443134 5 69 gly observed 1049:1056 arg2 a ubiquitin-associated protein AND O-GlcNAc modification a ubiquitin-associated protein O-GlcNAc modification PUBTATOR ubiquitin-associated protein 100286392 O-GlcNAc modification was observed on enzymes involved in post-translational regulation, including MAST4 and WNK1 kinases, a ubiquitin-associated protein (UBAP2l), and the histone acetyltransferase CREB-binding protein. 11038011 10 73 gly glycosylation 1975:1987 arg1 vasopressin vasopressin PUBTATOR vasopressin 551 Our data suggest that 1) intact neurophysin is not indispensable for vasopressin expression, although an altered structure of neurophysin significantly affects the secretion of the hormone; 2) the pathogenesis of diabetes insipidus with the two naturally-occurring mutations found in the rat (Brattleboro rat) and human (familial central diabetes insipidus) seem to be different; and 3) glycosylation of the carboxy-terminal glycopeptide is not essential for the expression of vasopressin. 7062029 7 57 gly unglycosylated 1388:1401 arg1 the unglycosylated P0 the unglycosylated P0 Cterm P0 At this time it is not possible to determine whether the unglycosylated P0 is actually assembled into a site and configuration like that of P0. 25512553 4 12 part_of SERT 662:665 arg1 Cys200 and Cys209 residues SERT Cys200 and Cys209 residues PUBTATOR AminoAcid SERT 6532 Cys200 and Cys209 residues We previously reported that an endoplasmic reticulum chaperone, ERp44, binds to Cys200 and Cys209 residues of SERT to build a disulfide bond. 28509333 1 56 gly glycosylation 174:186 arg1 native immunoglobulin (Ig)G native immunoglobulin (Ig)G Cterm (Ig)G The goal of this study was to investigate the glycosylation profile of native immunoglobulin (Ig)G present in serum immune complexes in patients with rheumatoid arthritis (RA). 9201957 2 49 gly glycoprotein 285:296 arg1 Pulmonary surfactant protein A Pulmonary surfactant protein A OGER protein A Q86XJ0 Pulmonary surfactant protein A (SP-A), an alveolar glycoprotein containing collagen-like and carbohydrate recognition domains (CRD), binds P. carinii and enhances adherence to alveolar macrophages. 9201957 2 44 gly containing 298:307 arg1 Pulmonary surfactant protein A AND collagen-like and carbohydrate recognition domains Pulmonary surfactant protein A collagen-like and carbohydrate recognition domains OGER protein A Q86XJ0 Pulmonary surfactant protein A (SP-A), an alveolar glycoprotein containing collagen-like and carbohydrate recognition domains (CRD), binds P. carinii and enhances adherence to alveolar macrophages. 25755023 0 62 gly glycoprotein 62:73 arg1 the tumor-associated MUC4 glycoprotein the tumor-associated MUC4 glycoprotein PUBTATOR MUC4 glycoprotein 140474 Antibody induction directed against the tumor-associated MUC4 glycoprotein. 2605214 3 48 gly Lol 625:627 arg1 another L. perenne allergen Lol p II another L. perenne allergen OGER Lol p II Q08397 The sequence of Lol p III is very similar to that of another L. perenne allergen, Lol p II, which was sequenced recently; of the 97 positions in the two proteins, 57 are occupied by identical amino acids (59% identity). 11733580 0 5 gly O-glycans 11:19 arg1 CC chemokine receptor 5 CC chemokine receptor 5 O-glycans PUBTATOR CC chemokine receptor 5 1234 Sialylated O-glycans and sulfated tyrosines in the NH2-terminal domain of CC chemokine receptor 5 contribute to high affinity binding of chemokines. 8275954 4 77 gly nonglycosylated 779:793 arg1 nonglycosylated recombinant PL-I nonglycosylated recombinant PL-I PUBTATOR PL-I 53950 Using an affinity column composed of monoclonal antibody to rPL-I coupled to Sepharose 4B, we have purified rPL-I from four sources: 1) recombinant rPL-I produced and secreted in rPL-I-transfected CHO cells, 2) nonglycosylated recombinant PL-I produced by adding tunicamycin (10 microM/ml medium) to rPL-I-transfected CHO cells, 3) native rPL-I secreted by rat choriocarcinoma (RCHO) cells, and 4) serum rPL-I isolated from day 12 pregnant rats. 1730732 6 55 gly glycosylation 982:994 arg1 S-laminin S-laminin OGER laminin Analysis of tunicamycin-treated cells indicated that N-linked glycosylation is required neither for the selective association of S-laminin with B2 and A subunits nor for the distinction between two forms of S-laminin. 8357534 2 39 part_of has 374:376 arg1 cathepsin G AND Asn-64 cathepsin G Asn-64 PUBTATOR SpecificSite cathepsin G 1511 Asn-64 Elastase has two N-glycosylation sites occupied (Asn-45 and Asn-144), whereas cathepsin G has only one (Asn-64). 9852066 0 52 gly O-glycosylation 20:34 arg1 CGA79-439 CGA79-439 Cterm CGA79-439 1113 Phosphorylation and O-glycosylation sites of human chromogranin A (CGA79-439) from urine of patients with carcinoid tumors. 9852066 0 52 gly O-glycosylation 20:34 arg1 human chromogranin A human chromogranin A PUBTATOR chromogranin A 1113 Phosphorylation and O-glycosylation sites of human chromogranin A (CGA79-439) from urine of patients with carcinoid tumors. 12438572 1 59 gly glycoprotein 110:121 arg1 GP GP Cterm GP The role of covalent modifications of the Ebola virus glycoprotein (GP) and the significance of the sequence identity between filovirus and avian retrovirus GPs were investigated through biochemical and functional analyses of mutant GPs. 20943674 0 46 gly N-glycosylation 52:66 arg1 wild-type and recombinant human lactoferrin wild-type and recombinant human lactoferrin OGER lactoferrin P02788 Comprehensive characterization of the site-specific N-glycosylation of wild-type and recombinant human lactoferrin expressed in the milk of transgenic cloned cattle. 16085713 2 29 gly eNOS 416:419 arg1 O-GlcNAc modification eNOS O-GlcNAc modification OGER eNOS Q62600 We investigated whether hyperglycemia increases O-GlcNAc modification of eNOS in the penis, preventing phosphorylation at the primary positive regulatory site on the enzyme and hampering mechanisms of the erectile response. 16085713 2 48 gly modification 400:411 arg1 eNOS AND O-GlcNAc modification eNOS O-GlcNAc modification OGER eNOS Q62600 We investigated whether hyperglycemia increases O-GlcNAc modification of eNOS in the penis, preventing phosphorylation at the primary positive regulatory site on the enzyme and hampering mechanisms of the erectile response. 1991473 6 48 part_of hLH 817:819 arg1 Asn30 hLH beta Asn30 PUBTATOR AminoAcid hLH beta 3972 Asn30 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 1991473 6 60 part_of hLH 795:797 arg1 Asn52 hLH alpha Asn52 PUBTATOR AminoAcid hLH alpha 1081 Asn52 The oligosaccharides attached to Asn52 (hLH alpha) and Asn30 (hLH beta) show a remarkably similar pattern, with mainly chain-terminating 4-sulphated 2-deoxy-2-N-acetylamino-D-galactose (GalNAc) and a sulphated/sialylated structure as the major single component. 11805097 7 7 gly glycoprotein 1037:1048 arg1 Punctin Punctin PUBTATOR Punctin 92949 Punctin is a glycoprotein based on carbohydrate staining and liquid chromatography electrospray mass spectrometry glycopeptide analysis. 10037148 0 30 gly glycoprotein 43:54 arg1 the myelin-associated glycoprotein the myelin-associated glycoprotein PUBTATOR myelin-associated glycoprotein 17136 Binding partners for the myelin-associated glycoprotein of N2A neuroblastoma cells. 7849028 3 58 gly Glycosylation 282:294 arg1 p62 p62 PUBTATOR p62 117268 Glycosylation of the major rat nuclear pore glycoprotein, p62, was examined in vitro using recombinant p62 as a substrate. 7849028 3 102 gly glycoprotein 326:337 arg1 p62 p62 PUBTATOR p62 117268 Glycosylation of the major rat nuclear pore glycoprotein, p62, was examined in vitro using recombinant p62 as a substrate. 9394011 8 20 gly glycosylated 1448:1459 arg1 CD8/E19 CD8/E19 PUBTATOR ER, CD8/E19 925 However, upon relocation of chimeric GalNAc-T1 or -T2 to the ER, CD8/E19 is glycosylated with different efficiencies indicating that all components required for initiation of O-glycosylation are present in the ER except for polypeptide GalNAc-transferases. 11086118 1 82 gly glycoproteins 218:230 arg1 E2(660) E2(660) Cterm E2 We compared the ability of two closely related truncated E2 glycoproteins (E2(660)) derived from hepatitis C virus (HCV) genotype 1a strains Glasgow (Gla) and H77c to bind a panel of conformation-dependent monoclonal antibodies (MAbs) and CD81. 2113057 3 81 gly glycosylation 955:967 arg1 tPA tPA PUBTATOR tPA 5327 Since this value was not significantly different for the different tcwt-rtPA preparations, it appears as though the nature of the glycosylation of tPA plays little role in its initial interaction with PAI-1. 22178065 0 23 gly glycosylated 93:104 arg1 glycosylated PAI-1 glycosylated PAI-1 PUBTATOR PAI-1 18787 Maximal PAI-1 inhibition in vivo requires neutralizing antibodies that recognize and inhibit glycosylated PAI-1. 25707740 16 71 gly glycosylated 1824:1835 arg1 Other abnormally glycosylated hCG Other abnormally glycosylated hCG OGER hCG Other abnormally glycosylated hCG are described in aneuploidies. 17715132 5 1 gly glycosylated 694:705 arg1 Pannexin1 Pannexin1 PUBTATOR Pannexin1 24145 We show for the first time that Pannexin1 is glycosylated at Asn-254 and that this residue is important for plasma membrane targeting. 9278435 0 79 gly alpha-L-iduronidase 45:63 arg1 Carbohydrate structures alpha-L-iduronidase Carbohydrate structures PUBTATOR alpha-L-iduronidase 3425 Carbohydrate structures of recombinant human alpha-L-iduronidase secreted by Chinese hamster ovary cells. 15728186 8 82 gly glycoforms 1619:1628 arg1 myeloma IgA1 HR glycoforms myeloma IgA1 HR glycoforms OGER IgA1 P01876 Multiple sites of O-glycan attachment (including sites of Gal deficiency) in myeloma IgA1 HR glycoforms were identified (in all but one case uniquely). 22164239 7 100 gly sialylation 1179:1189 arg1 VL VL Cterm VL Although the presence of both N- and O-glycosylations was found both in spectrin(N) and spectrin(VL), enhanced sialylation was predominantly induced in spectrin(VL). 1396319 6 7 gly present 728:734 arg2 the PRL receptor AND The carbohydrate linkages the PRL receptor The carbohydrate linkages OGER PRL receptor P40424 The carbohydrate linkages present in the PRL receptor were examined using enzymes to deglycosylate iodinated purified receptors. 16343775 3 108 gly glycoforms 621:630 arg1 human IgG1-Fc human IgG1-Fc OGER IgG1 P01857 To gain a better understanding of the role of Fc glycosylation, we prepared a series of truncated glycoforms of human IgG1-Fc and analyzed their interactions with human soluble FcgammaRIIIa (sFcgammaRIIIa) and with staphylococcal protein A by surface plasmon resonance and nuclear magnetic resonance (NMR) methods. 12087059 7 58 gly N-glycosylated 1469:1482 arg1 the N-glycosylated Edg-1 the N-glycosylated Edg-1 PUBTATOR Edg-1 1901 Although the precise molecular mechanism of the internalization of the N-glycosylated Edg-1 localized in the microdomain remains to be examined, the present study suggested that the presence of N-linked glycan in the receptor may play a regulatory role in the receptor dynamics in ligand-stimulated mammalian cells. 8105887 0 71 gly glycosylated 65:76 arg1 human CD2 human CD2 PUBTATOR CD2 914 1H resonance assignments and secondary structure of the 13.6 kDa glycosylated adhesion domain of human CD2. 17197010 0 39 gly glycosylation 9:21 arg1 Gn Gn Cterm Gn N-linked glycosylation of Gn (but not Gc) is important for Crimean Congo hemorrhagic fever virus glycoprotein localization and transport. 17176047 3 43 gly B 418:418 arg1 The many O-linked glycans gelatinase B The many O-linked glycans OGER gelatinase B P14780 The many O-linked glycans of neutrophil gelatinase B presented a cluster of mainly galactosylated core II structures, 46% of which were ligands for galectin-3; 11% contained two to three N-acetyllactosamine repeating units that are high-affinity ligands for the lectin. 16439062 10 68 gly glycosylated 1729:1740 arg1 glycosylated interferon-beta glycosylated interferon-beta PUBTATOR interferon-beta 100750347 Furthermore, glycosylated interferon-beta, expressed in Chinese hamster ovary cells, was more resistant to this proteolysis than recombinant interferon-beta from bacteria. 8206884 6 1 gly carries 760:766 arg1 Recombinant hIL-6 AND one O-linked carbohydrate chain Recombinant hIL-6 one O-linked carbohydrate chain PUBTATOR hIL-6 3569 Recombinant hIL-6 carries one O-linked carbohydrate chain, and Thr139 is fully O-glycosylated. 20511397 2 13 gly glycosylated/sialylated 523:545 arg1 plasma apoE plasma apoE PUBTATOR apoE 348 Cellular apoE and plasma apoE exist as multiple glycosylated and sialylated glycoforms with plasma apoE being less glycosylated/sialylated than cell-derived apoE. 16880503 14 40 gly glycan 2027:2032 arg1 Ser-132 Ser-132 Ser-132 SpecificSite Ser-132 Analysis of hCGh affinity purified with antibody B152 confirmed that this antibody recognizes a core-2 glycan on Ser-132. 17711303 1 60 gly glycosylation 144:156 arg1 FLAG-hKOR FLAG-hKOR PUBTATOR hKOR 4986 We examined glycosylation of FLAG-hKOR expressed in CHO cells and determined its functional significance. 7628611 1 5 gly glycosylated 209:220 arg1 KBPs KBPs Cterm KBPs 26128 Kainate binding proteins (KBPs) from frog and goldfish brain are glycosylated, integral membrane proteins. 16407296 4 62 gly N-glycosylated 509:522 arg1 a typical Wnt a typical Wnt PUBTATOR Wnt 7482 Wnt13A, a typical Wnt, is N-glycosylated and localized in the endoplasmic reticulum, with only a small fraction being secreted. 10561463 1 1 gly glycoprotein 165:176 arg1 Thy-1 Thy-1 PUBTATOR Thy-1 100758237 Thy-1 is a cell surface glycoprotein containing three N-linked glycosylation sites and a glycosylphosphatidylinositol (GPI) anchor. 8502242 4 6 gly IgG2b 619:623 arg1 Additionally aglycosylated mutants IgG2b Additionally aglycosylated mutants PUBTATOR IgG2b 16016 Additionally aglycosylated mutants of this IgG4 (B72.3) and anti-NIP mouse IgG2b were analysed. 8502242 4 66 gly IgG4 587:590 arg1 Additionally aglycosylated mutants IgG4 Additionally aglycosylated mutants OGER IgG4 P01861 Additionally aglycosylated mutants of this IgG4 (B72.3) and anti-NIP mouse IgG2b were analysed. 28624365 7 26 gly APP 966:968 arg1 three predicted O-GlcNAc modification threonine residues APP three predicted O-GlcNAc modification threonine residues OGER APP P05067 In this study, we mutated three predicted O-GlcNAc modification threonine residues of APP into alanines (T291A, T292A, and T576A) and expressed them in HeLa cells. 23389048 0 97 gly glycoforms 120:129 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Quantitative liquid chromatography-mass spectrometry-multiple reaction monitoring (LC-MS-MRM) analysis of site-specific glycoforms of haptoglobin in liver disease. 8798614 3 69 part_of CD59 619:622 arg1 Asn18 CD59 Asn18 PUBTATOR AminoAcid CD59 966 Asn18 The deduced amino acid sequences of CD59 homologues identified in Old and New World primates as well as in rat reveal that the motif for N-linked glycosylation at the residue corresponding to Asn18 of human CD59 is invariably conserved, despite considerable sequence divergence elsewhere in the protein. 2066676 0 66 gly glycosylation 19:31 arg1 hepatic lipase activity hepatic lipase activity PUBTATOR lipase 291437 Effect of N-linked glycosylation on hepatic lipase activity. 15944403 11 57 gly determinant 1784:1794 arg1 SI SI determinant PUBTATOR SI 6476 We propose that the phenylalanine cluster is required for shielding a folding determinant in the extracellular domain of SI; substitution of a Q by a P at residue 1098 of sucrase disrupts this determinant and elicits retention of SI(Q1098P) in ERGIC and cis-Golgi in phenotype II of CSID. 9049331 8 35 gly glycosylation 1442:1454 arg1 the E2 protein the E2 protein PUBTATOR E2 protein 7320 Our results suggest that O-linked glycosylation on the E2 protein occurred in the post-ER region and the transport of RV structural proteins to the Golgi complex and post-Golgi compartment may be a rate-limiting step in RV assembly and budding. 20421293 1 24 gly L11 160:162 arg1 The lipooligosaccharide L11 The lipooligosaccharide OGER L11 Q9H910 The lipooligosaccharide (LOS) of immunotype L11 is unique within serogroup A meningococci. 30157810 3 97 gly carbohydrate-modifying 349:370 arg1 PGM3 phosphoglucomutase 3 PGM3 PUBTATOR phosphoglucomutase 3 5238 PGM3 encodes a carbohydrate-modifying enzyme, phosphoglucomutase 3. 7722516 2 30 gly nonglycosylated 460:474 arg1 pro-7B2 7B2 PUBTATOR AminoAcid 7B2 6447 The endocrine and neural protein 7B2 is first synthesized as a nonglycosylated precursor (pro-7B2), which is cleaved within the TGN by a furin-like ubiquitous convertase at the RRKRR155S site to generate 7B2. 20573835 2 26 gly glycoprotein 495:506 arg1 SARS-S SARS-S Cterm SARS-S The objective of this study was to assess the interactions between MBL and severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein (SARS-S). 8609471 5 47 part_of C-E1-truncated 721:734 arg1 residues 132-383 E1 residues 132-383 Cterm SpecificSite E1 residues 132-383 However, when E1 was expressed as part of a truncated C-E1-truncated E2 polypeptide (residues 132-383), the processed E1 product had the expected apparent molecular mass of 31 kDa, suggesting that flanking sequences are necessary for the generation of the mature 31 kDa El form. 8702840 6 7 gly modified 704:711 arg3 NF-H AND O-GlcNAc NF-H O-GlcNAc PUBTATOR NF-H 4744 Here we further report that NF-H is extensively modified by O-GlcNAc at Thr53, Ser54, and Ser56 in the head domain and, somewhat surprisingly, at multiple sites within the Lys-Ser-Pro repeat motif in the tail domain, a region in assembled neurofilaments known to be nearly stoichiometrically phosphorylated on each of the approximately 50 KSP repeats. 10483920 10 61 part_of PrP 1284:1286 arg1 the polymorphic residue 129 PrP the polymorphic residue 129 PUBTATOR SpecificSite PrP 5621 residue 129 Our findings indicate that the polymorphic residue 129 of PrP has a leading role in determining the proteinase degradation site of PrPsc while mutant residues 102 or 200 influence only the glycosylation pattern. 26536155 6 38 gly glycosylation 1501:1513 arg1 human IgG3 human IgG3 PUBTATOR IgG3 3502 The approach was evaluated on glycoprotein standards and also applied to investigate the glycosylation of human IgG3 providing details on the hitherto uncharacterized glycosylation site Asn392 of the CH3 domain. 16871372 0 34 gly alpha1-antitrypsin 46:63 arg1 oligosaccharides alpha1-antitrypsin oligosaccharides PUBTATOR alpha1-antitrypsin 5265 Endomannosidase processes oligosaccharides of alpha1-antitrypsin and its naturally occurring genetic variants in the Golgi apparatus. 16871372 0 34 gly alpha1-antitrypsin 46:63 arg1 its naturally occurring genetic variants alpha1-antitrypsin its naturally occurring genetic variants PUBTATOR alpha1-antitrypsin 5265 Endomannosidase processes oligosaccharides of alpha1-antitrypsin and its naturally occurring genetic variants in the Golgi apparatus. 16407218 5 66 gly MBL 1009:1011 arg1 carbohydrate recognition domains MBL carbohydrate recognition domains PUBTATOR MBL 4153 We investigated the mechanism of formation of complexes between alpha2M and MBL and concluded that they form by the direct binding of oligomannose glycans Man(5-7) occupying Asn-846 on alpha2M to the lectin domains (carbohydrate recognition domains) of MBL. 28230186 3 8 gly Glycosylation 347:359 arg1 IgG IgG Cterm IgG Glycosylation of IgG is known to modulate the effector functions of Fcγ receptors. 2312359 2 87 gly glycoproteins 175:187 arg1 mucin glycoproteins mucin glycoproteins PUBTATOR mucin glycoproteins 100508689 Lectins were used to characterize mucin glycoproteins and other secretory glycoconjugates synthesized by a human colon adenocarcinoma-derived cell line which expresses a goblet cell phenotype. 19800385 3 40 gly attached 298:305 arg1 IGFBP-3 AND N-glycans IGFBP-3 N-glycans PUBTATOR IGFBP-3 3486 The aim of this study was to characterise N-glycans covalently attached to IGFBP-3 from sera of healthy adults (men and women). 8349827 1 35 gly glycoprotein 234:245 arg1 Glycosylation-dependent cell adhesion molecule 1 Glycosylation-dependent cell adhesion molecule 1 PUBTATOR Glycosylation-dependent cell adhesion molecule 1 14663 Glycosylation-dependent cell adhesion molecule 1 (GlyCAM 1) is a mucinlike endothelial glycoprotein that acts as an adhesive ligand for L selectin by presenting one or more O-linked carbohydrates to the lectin domain of this leukocyte cell surface selectin. 9259320 7 50 gly O-glycosylation 1174:1188 arg1 DSBP DSBP Cterm DSBP 26386 Using lectin-affinity chromatography, discordance between the pattern of O-glycosylation of SSBP and DSBP was demonstrated. 9259320 7 50 gly O-glycosylation 1174:1188 arg1 SSBP SSBP OGER SSBP Q04837 Using lectin-affinity chromatography, discordance between the pattern of O-glycosylation of SSBP and DSBP was demonstrated. 20512979 5 0 gly glycoprotein 924:935 arg1 HGF/SF HGF/SF PUBTATOR HGF/SF 100764472 We exemplify the strategy by describing novel clones expressing single-chain hepatocyte growth factor/scatter factor (HGF/SF, a secreted glycoprotein) and a domain of lysosome-associated membrane protein 3 (LAMP3d). 27350215 2 25 gly C-mannosylation 308:322 arg1 human Rspo3 human Rspo3 PUBTATOR Rspo3 84870 In this study, we focused on C-mannosylation, a unique type of glycosylation, of human Rspo3. 27350215 2 29 gly Rspo3 366:370 arg1 C-mannosylation Rspo3 C-mannosylation PUBTATOR Rspo3 84870 In this study, we focused on C-mannosylation, a unique type of glycosylation, of human Rspo3. 28150441 7 24 gly glycoprotein 1190:1201 arg1 EuP-82 EuP-82 Cterm EuP-82 2147 The SR-FTIR spectra revealed that EuP-82 was a glycoprotein. 16716077 0 51 gly glycosylation 10:22 arg1 a soluble, recombinant form a soluble, recombinant form PUBTATOR form of the transferrin receptor 7037 Effect of glycosylation on the function of a soluble, recombinant form of the transferrin receptor. 29408166 5 75 gly core-fucosylated 1174:1189 arg1 core-fucosylated PSA core-fucosylated PSA PUBTATOR PSA 354 The method was shown to be linear from 0.5 to 60 ng/ml total PSA concentrations and allows the simultaneous quantification of core-fucosylated PSA down to 1 ng/ml and total PSA lower than 0.5 ng/ml. 12794129 1 26 gly attached 119:126 arg2 IgM AND a small polypeptide IgM a small polypeptide OGER IgM P01871 J chain is a small polypeptide covalently attached to polymeric IgA and IgM. 12794129 1 26 gly attached 119:126 arg1 IgA AND a small polypeptide IgA a small polypeptide OGER IgA P11912 J chain is a small polypeptide covalently attached to polymeric IgA and IgM. 12794129 1 38 gly J 77:77 arg1 a small polypeptide J chain a small polypeptide PUBTATOR J chain 3512 J chain is a small polypeptide covalently attached to polymeric IgA and IgM. 14749323 8 5 gly glycosylation 1484:1496 arg1 OAT function OAT function OGER OAT P04181 Single replacement of asparagines at other sites had no effect on transport activity indicating that glycosylation at individual sites is not essential for OAT function. 10400680 8 56 gly AChR 1218:1221 arg1 all four subunits AChR all four subunits OGER AChR When all four subunits of the AChR (alpha, beta, delta, and epsilon) were coexpressed, mutation of the delta subunit to prevent glycosylation resulted in a reduced amount of fully assembled AChR and reduced surface AChR levels, consistent with the role of the heterodimer in the assembly reaction. 1391595 0 116 gly residues 25:32 arg1 Tg Tg residues Cterm Tg 7038 Analysis of carbohydrate residues on human thyroid peroxidase (TPO) and thyroglobulin (Tg) and effects of deglycosylation, reduction and unfolding on autoantibody binding. 1391595 0 116 gly residues 25:32 arg1 TPO TPO residues PUBTATOR TPO 7173 Analysis of carbohydrate residues on human thyroid peroxidase (TPO) and thyroglobulin (Tg) and effects of deglycosylation, reduction and unfolding on autoantibody binding. 1391595 0 116 gly residues 25:32 arg1 thyroglobulin thyroglobulin residues PUBTATOR thyroglobulin 7038 Analysis of carbohydrate residues on human thyroid peroxidase (TPO) and thyroglobulin (Tg) and effects of deglycosylation, reduction and unfolding on autoantibody binding. 1391595 0 116 gly residues 25:32 arg1 human thyroid peroxidase thyroid peroxidase residues PUBTATOR thyroid peroxidase 7173 Analysis of carbohydrate residues on human thyroid peroxidase (TPO) and thyroglobulin (Tg) and effects of deglycosylation, reduction and unfolding on autoantibody binding. 15807535 7 77 gly glycosylated 1346:1357 arg1 the glycosylated ABCG2 protein the glycosylated ABCG2 protein PUBTATOR ABCG2 protein 9429 Cell surface analysis of ABCG2 expression showed comparable amounts of the N596Q variant present at the plasma membrane compared to the glycosylated ABCG2 protein. 16861659 0 51 gly glycoprotein 39:50 arg1 Cj1496c Cj1496c Cterm Cj1496c Cj1496c encodes a Campylobacter jejuni glycoprotein that influences invasion of human epithelial cells and colonization of the chick gastrointestinal tract. 2676155 3 139 gly glycoprotein 727:738 arg1 LAMP-1 LAMP-1 PUBTATOR LAMP-1 16783 This glycoprotein has recently been purified from the metastatic MDAY-D2 cell line and shown to be biochemically similar to a lysosomal associated membrane glycoprotein (LAMP-1). 20670608 4 57 gly deglycosylated 649:662 arg1 Recombinant and deglycosylated HCII Recombinant and deglycosylated HCII OGER HCII P05546 Recombinant and deglycosylated HCII bound heparin with dissociation constants (K(D)) of 6+/-1 and 7+/-1 microM, respectively, approximately 6-fold tighter than plasma HCII, with K(D) 40+/-4 microM. 21637915 2 45 gly glycosylated 238:249 arg1 Emmprin Emmprin PUBTATOR Emmprin 682 Emmprin is a glycosylated transmembrane protein containing two immunoglobulin (Ig) domains that is expressed in carcinoma cells and stimulates MMP production by adjacent stromal cells. 23776650 3 21 gly Immunoreceptor 447:460 arg1 glycan specificity Dendritic Cell Immunoreceptor glycan specificity OGER Dendritic Cell Immunoreceptor Q9UMR7 Little is known on the glycan specificity and ligands of the Dendritic Cell Immunoreceptor (DCIR), the only classical C-type lectin that contains an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM). 23815085 4 8 gly deglycosylation 574:588 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 Following deglycosylation of TIMP-1, all forms of TIMP-1 showed similar levels of MMP binding and inhibition, suggesting that glycosylation is involved in the regulation of these TIMP-1 activities. 23815085 4 43 gly TIMP-1 614:619 arg1 all forms TIMP-1 all forms PUBTATOR TIMP-1 7076 Following deglycosylation of TIMP-1, all forms of TIMP-1 showed similar levels of MMP binding and inhibition, suggesting that glycosylation is involved in the regulation of these TIMP-1 activities. 3339090 1 12 gly glycoprotein 171:182 arg1 LEP100 LEP100 PUBTATOR LEP100 396220 LEP100, a membrane glycoprotein that has the unique property of shuttling from lysosomes to endosomes to plasma membrane and back, was purified from chicken brain. 15024013 4 61 gly glycosylation 854:866 arg1 DAT expression DAT expression PUBTATOR DAT 6531 Although partially or non-glycosylated DAT was somewhat less represented at the surface, no evidence was found for preferential exclusion of such material from the plasma membrane, indicating that glycosylation is not essential for DAT expression. 17389369 4 7 gly Fbs1-glycoprotein 607:623 arg1 the Fbs1-glycoprotein complex the Fbs1-glycoprotein complex PUBTATOR Fbs1 26232 To elucidate the structural basis of SCF(Fbs1) function, we determined the crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain (SBD) of the Fbs1-glycoprotein complex. 23187000 5 44 gly glycosylation 880:892 arg1 TIMP-1 TIMP-1 PUBTATOR TIMP-1 7076 Because the TIMP-1 glycosylation participate in the interaction, aberrant glycosylation of TIMP-1 presumably affects the interaction, thereby leading to pathogenic dysfunction in cancer cells. 18209065 4 5 gly chains 716:721 arg1 CD99 CD99 chains PUBTATOR CD99 673094 We herein report that sialylated O-linked sugar chains on CD99 are essential for the recognition by PILR. 2573604 0 66 gly glycosylation 9:21 arg1 rat renal gamma-glutamyltranspeptidase rat renal gamma-glutamyltranspeptidase PUBTATOR gamma-glutamyltranspeptidase 116568 O-linked glycosylation of rat renal gamma-glutamyltranspeptidase adjacent to its membrane anchor domain. 18045109 8 50 gly glycoprotein 1542:1553 arg1 the HIV envelope glycoprotein the HIV envelope glycoprotein PUBTATOR HIV envelope glycoprotein 100616444 Nevertheless, the hope is that through novel insights and creative solutions that we will be able to design immunogens capable of eliciting broadly neutralizing antibodies to the HIV envelope glycoprotein. 11389975 0 47 gly Glycosylation 0:12 arg1 human CRLR human CRLR PUBTATOR CRLR 10203 Glycosylation of human CRLR at Asn123 is required for ligand binding and signaling. 21613225 3 84 gly factor 481:486 arg1 the mannose phosphorylation status leukemia inhibitory factor the mannose phosphorylation status PUBTATOR leukemia inhibitory factor 3976 In this study, we investigated the mannose phosphorylation status of leukemia inhibitory factor (LIF), a previously identified high affinity ligand for the cation-independent mannose 6-phosphate receptor (CI-MPR), and we analyzed the effects of this modification on its secretion and uptake in cultured cells. 28630087 10 54 gly glycosylation 1662:1674 arg1 HNE HNE PUBTATOR HNE 1991 The three glycosylation sites of HNE were located distal to the active site indicating glycan functions other than interference with HNE enzyme activity. 25582524 3 31 gly glycoforms 490:499 arg1 the IgG glycoforms the IgG glycoforms Cterm IgG Glycan oxonium ions and peptide-GlcNAc fragment ions were utilized to quantify the IgG glycoforms purified by affinity chromatography with normalization to the unique peptide for each IgG subclass. 19192250 6 3 gly oligosaccharides 1074:1089 arg1 cystatin F cystatin F oligosaccharides PUBTATOR cystatin F 8530 These studies identify a function for the oligosaccharides on cystatin F and raise the possibility that cystatin F might regulate proteases in trans by secretion in an inactive form by one cell and subsequent internalization and activation by another cell. 9184148 8 55 gly alpha-antithrombin 1535:1552 arg1 the Asn-135 oligosaccharide antithrombin the Asn-135 oligosaccharide PUBTATOR antithrombin 462 These and previous results suggest a model in which the Asn-135 oligosaccharide of alpha-antithrombin is oriented away from the heparin binding site and does not interfere with the first step of heparin binding. 2026164 7 6 gly glycosylation 746:758 arg1 its N-terminal light chain its N-terminal light chain OGER chain 292858 Kallikrein k10 is microheterogeneous due to variable glycosylation of its N-terminal light chain and to variable processing at its kallikrein loop, as shown by endo-beta-N-acetylglucosaminidase F treatment, amino acid sequence analysis and mass spectrometry. 28630087 0 55 gly N-glycosylation 18:32 arg1 Spatiotemporally Regulated Human Neutrophil Elastase Spatiotemporally Regulated Human Neutrophil Elastase PUBTATOR Human Neutrophil Elastase 1991 Paucimannose-Rich N-glycosylation of Spatiotemporally Regulated Human Neutrophil Elastase Modulates Its Immune Functions. 28630087 0 122 gly Elastase 81:88 arg1 Paucimannose-Rich N-glycosylation Human Neutrophil Elastase Paucimannose-Rich N-glycosylation PUBTATOR Human Neutrophil Elastase 1991 Paucimannose-Rich N-glycosylation of Spatiotemporally Regulated Human Neutrophil Elastase Modulates Its Immune Functions. 7929395 0 119 gly glycosylation 36:48 arg1 fibrillin-1 fibrillin-1 PUBTATOR fibrillin-1 2200 Calcium binding, hydroxylation, and glycosylation of the precursor epidermal growth factor-like domains of fibrillin-1, the Marfan gene protein. 7354085 0 21 gly sialoglycoprotein 70:86 arg1 glycophorin A glycophorin A PUBTATOR glycophorin A, 2993 Effect of tunicamycin on the biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in the leukemia cell line K562. 18076768 0 32 part_of Asn 0:2 arg1 gp120 gp120 Asn PUBTATOR SpecificSite gp120 155971 Asn 362 Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS. 23808883 7 71 gly glycosylated 1275:1286 arg1 differentially glycosylated IgGs differentially glycosylated IgGs Cterm IgGs Under physiological conditions, differentially glycosylated IgGs mediate their pro- or anti-inflammatory effector functions obviously as immune complexes (IC) in an antigen-specific manner. 3594570 1 27 gly glycoproteins 261:273 arg1 Mac-1 Mac-1 PUBTATOR Mac-1 3689 Leukocyte adhesion deficiency (LAD) is a heritable disease involving deficient expression of three related leukocyte adhesion glycoproteins: LFA-1, Mac-1, and p150,95. 3594570 1 27 gly glycoproteins 261:273 arg1 p150,95 p150,95 PUBTATOR p150 10036 Leukocyte adhesion deficiency (LAD) is a heritable disease involving deficient expression of three related leukocyte adhesion glycoproteins: LFA-1, Mac-1, and p150,95. 3594570 1 27 gly glycoproteins 261:273 arg1 LFA-1 LFA-1 PUBTATOR LFA-1 3683 Leukocyte adhesion deficiency (LAD) is a heritable disease involving deficient expression of three related leukocyte adhesion glycoproteins: LFA-1, Mac-1, and p150,95. 11027492 9 22 gly deglycosylation 1439:1453 arg1 native 11beta-HSD 1 native 11beta-HSD 1 PUBTATOR 11beta-HSD 1 3290 By stepwise enzymatic deglycosylation of native 11beta-HSD 1 we could demonstrate that all potential glycosylation sites carry N-linked oligosaccharide residues under physiological conditions. 24946017 8 71 gly glycoproteins 1197:1209 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 A few glycoproteins, including vitronectin, showed significantly different site-specific glycosylations within cancer/control samples, indicating that our method is ready to be used for the discovery of glycosylated biomarkers. 25211026 2 136 gly sialoglycoprotein 347:363 arg1 Pr Pr PUBTATOR Pr(PC 19122 Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites. 21978954 6 21 gly glycoforms 1180:1189 arg1 minor IgG1 glycoforms minor IgG1 glycoforms OGER IgG1 P01857 Enhanced detection for minor IgG1 glycoforms (∼0.1 to 1.0 mol% level) was obtained by LC-MS of the longer 32-residue Asp-N glycopeptide (4+ protonated ion) compared to the 9-residue tryptic glycopeptide (2+ ion). 7795219 5 20 gly Deglycosylation 719:733 arg1 HN HN Cterm HN Deglycosylation of HN with trifluoromethanesulfonic acid, which cleaves N- and O-linked oligosaccharides, inhibits 66% of cell attachment to HN, and results in an apparent decrease in molecular weight from 60 to 50 kD. 15054092 4 55 gly glycosylated 624:635 arg1 G5 G5 Cterm G5 100761230 Site-directed mutagenesis revealed that two asparagine residues (Asn(585) and Asn(592)) are glycosylated in G5 and that G8 has a single N-linked glycan attached to Asn(619). 15054092 4 33 gly has 655:657 arg1 G8 AND a single N-linked glycan G8 a single N-linked glycan Cterm G8 100765601 Site-directed mutagenesis revealed that two asparagine residues (Asn(585) and Asn(592)) are glycosylated in G5 and that G8 has a single N-linked glycan attached to Asn(619). 28327546 3 81 gly N-glycosylation 340:354 arg1 FVIII FVIII PUBTATOR FVIII 2157 Here we characterize how hemophilia mutations near the unused N-glycosylation site of the A2 domain (N582) of FVIII affect protein conformation and intracellular trafficking. 28835497 2 89 gly glycoprotein 256:267 arg1 Glycoprotein K Glycoprotein K Cterm Glycoprotein K Glycoprotein K (gK) is a conserved virion glycoprotein of all alphaherpesviruses that is not found in other herpesvirus subfamilies. 2972716 4 72 gly deglycosylated 484:497 arg1 deglycosylated enzyme subunits deglycosylated enzyme subunits OGER subunits 10724 We used a homogeneous preparation of deglycosylated enzyme subunits to generate antibody. 10763868 1 8 gly glycoprotein 225:236 arg1 AGP AGP Cterm AGP HPLC chiral stationary phases based on human plasma alpha1-acid glycoprotein (AGP) and partially deglycosylated AGP (pd-AGP) were prepared to investigate the effects of sugar moiety of AGP on chiral discrimination of various solutes. 10763868 1 23 gly AGP 346:348 arg1 sugar moiety AGP sugar moiety Cterm AGP HPLC chiral stationary phases based on human plasma alpha1-acid glycoprotein (AGP) and partially deglycosylated AGP (pd-AGP) were prepared to investigate the effects of sugar moiety of AGP on chiral discrimination of various solutes. 10763868 1 36 gly deglycosylated 258:271 arg1 partially deglycosylated AGP AGP Cterm AGP HPLC chiral stationary phases based on human plasma alpha1-acid glycoprotein (AGP) and partially deglycosylated AGP (pd-AGP) were prepared to investigate the effects of sugar moiety of AGP on chiral discrimination of various solutes. 21673010 7 57 gly hormone 1015:1021 arg1 two N-glycan sites growth hormone two N-glycan sites PUBTATOR growth hormone 81668 For this purpose, rat growth hormone (rGH) with two N-glycan sites (rGH-2N) inserted into the rGH portion (NAS and NFT) was fused to green fluorescent protein (GFP) and expressed in MDCK cells. 10571021 1 6 gly contains 131:138 arg1 Human chorionic gonadotropin AND five acidic N-linked sugar chains Human chorionic gonadotropin (hCG) five acidic N-linked sugar chains PUBTATOR Human chorionic gonadotropin (hCG) 1081 Human chorionic gonadotropin (hCG) contains five acidic N-linked sugar chains, which are derived from three neutral oligosaccharides by sialylation. 8636209 8 53 gly glycosylation 1015:1027 arg1 TCR assembly TCR assembly PUBTATOR TCR 6962 In contrast, treatment of T cells with tunicamycin suggested that N-linked glycosylation of CD3 delta is required for TCR assembly. 8636209 8 53 gly glycosylation 1015:1027 arg1 CD3 delta CD3 delta PUBTATOR CD3 delta 915 In contrast, treatment of T cells with tunicamycin suggested that N-linked glycosylation of CD3 delta is required for TCR assembly. 18775496 3 2 gly Fucosylation 408:419 arg1 recombinant C45 agrin recombinant C45 agrin PUBTATOR C45 agrin 100765949 Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). 18775496 3 40 gly agrin 440:444 arg1 Fucosylation C45 agrin Fucosylation PUBTATOR C45 agrin 100765949 Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). 25265424 3 25 gly glycoforms 356:365 arg1 hemopexin hemopexin PUBTATOR hemopexin 3263 We present a case study that compares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. 25265424 3 25 gly glycoforms 356:365 arg1 kininogen-1 kininogen-1 PUBTATOR kininogen-1 3827 We present a case study that compares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. 25265424 3 25 gly glycoforms 356:365 arg1 complement factor H haptoglobin, complement factor H PUBTATOR haptoglobin, complement factor H 3240 We present a case study that compares site specific glycoforms of four proteins including haptoglobin, complement factor H, kininogen-1, and hemopexin isolated from the same patient. 10763868 8 30 gly AGP 1438:1440 arg1 A sugar chain(s) AGP A sugar chain(s) Cterm AGP A sugar chain(s) of AGP cleaved by N-glycosidase might be involved in the enantioselective binding of warfarin enantiomers. 25374123 3 56 gly N-glycopeptides 492:506 arg1 vitronectin vitronectin PUBTATOR vitronectin 7448 Here, we systematically analyzed the site-specific N-glycopeptides of vitronectin in human plasma by tandem mass spectrometry combined with immunoprecipitation and hydrophilic interaction liquid chromatography (HILIC) enrichment. 22154301 1 16 gly P-glycoprotein 104:117 arg1 The multidrug-resistance 1 (MDR-1) P-glycoprotein The multidrug-resistance 1 (MDR-1) P-glycoprotein PUBTATOR P-glycoprotein 5243 The multidrug-resistance 1 (MDR-1) P-glycoprotein (Pgp) is a transmembrane transporter system, which actively pumps cytotoxic drugs out of the cell. 22154301 1 16 gly P-glycoprotein 104:117 arg1 Pgp Pgp PUBTATOR Pgp 5243 The multidrug-resistance 1 (MDR-1) P-glycoprotein (Pgp) is a transmembrane transporter system, which actively pumps cytotoxic drugs out of the cell. 28150441 1 10 gly glycosylated 151:162 arg1 EuP-82 EuP-82 Cterm EuP-82 2147 In this study, the binding of a glycosylated serine protease (EuP-82) with human fibrinogen was investigated by isothermal titration calorimetry (ITC). 15478002 3 77 gly sALP 740:743 arg1 sialic acid content sALP sialic acid content OGER sALP O75525 sALP synthesis, glycosylation, and GPI-anchor attachment were assessed as total protein synthesis/immunospecific sALP synthesis, sialic acid content (i.e., wheat germ agglutinin precipitation), and insolubility (i.e., temperature-dependent phase-separation), respectively. 15478002 3 98 gly GPI-anchor 775:784 arg1 sialic acid content GPI sialic acid content PUBTATOR GPI 2821 sALP synthesis, glycosylation, and GPI-anchor attachment were assessed as total protein synthesis/immunospecific sALP synthesis, sialic acid content (i.e., wheat germ agglutinin precipitation), and insolubility (i.e., temperature-dependent phase-separation), respectively. 21976968 6 41 gly N-glycosylation 1033:1047 arg1 LRSH-labeled uromodulin LRSH-labeled uromodulin PUBTATOR LRSH-labeled uromodulin 7369 In addition, the N-glycosylation sites of LRSH-labeled uromodulin were readily mapped via in-gel PNGaseF deglycosylation and LC-MS/MS analysis, which indicated that this fluorescent dye labeling does not interfere with enzymatic deglycosylation. 10988252 9 12 gly glycosylation 1604:1616 arg1 sEGFR sEGFR Cterm sEGFR 1956 This first comprehensive glycosylation study on a human nonrecombinant receptor shows the immense heterogeneity of the glycosylation of sEGFR. 26013384 8 12 gly O-glycosylated 997:1010 arg1 fully O-glycosylated rHu-EPO fully O-glycosylated rHu-EPO PUBTATOR EPO 2056 It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126. 26013384 8 43 gly non-O-glycosylated 943:960 arg1 non-O-glycosylated rHu-EPO non-O-glycosylated rHu-EPO PUBTATOR EPO 2056 It is concluded that the variant peak is non-O-glycosylated rHu-EPO and the main peak is fully O-glycosylated rHu-EPO at Ser126. 7479385 4 94 gly contains 771:778 arg1 PSA AND a carbohydrate residue PSA a carbohydrate residue PUBTATOR PSA 354 The predominant PSA molecular species detected by ISMS was at relative molecular mass (M(r)) of 28,430, indicating that PSA contains a carbohydrate residue of M(r) 2,351, for a total percentage of carbohydrate of 8.3%. 17009075 7 44 gly IgG 1283:1285 arg1 the oligosaccharide structure IgG the oligosaccharide structure Cterm IgG CONCLUSIONS: The analysis of the oligosaccharide structure of serum IgG seems to be useful in diagnosing IgAN. 15047148 1 39 gly glycoprotein 118:129 arg1 CD28 CD28 PUBTATOR CD28 940 CD28 is a cell surface glycoprotein expressed on T cells that modulates immune responses through its ability to transduce costimulatory signals. 12223479 7 88 gly residues 1076:1083 arg1 beta4 beta4 residues PUBTATOR beta4 10717 Enzymatic deglycosylation or mutation of the N-linked glycosylation residues in beta4 converts it to a single lower molecular weight band, even in the presence of the hSlo alpha subunit, suggesting that the beta4 subunit can be present as an immature, core glycosylated form and a mature, highly glycosylated form. 8096511 2 38 gly P-glycoprotein 440:453 arg1 the human MDR1 P-glycoprotein the human MDR1 P-glycoprotein OGER MDR1 P-glycoprotein P08183 To investigate the significance of the conserved N-glycosylation sites present in the putative first extracellular loop of P-glycoproteins, we mutated one, two, or all three of these sites present in the human MDR1 P-glycoprotein. 9808768 13 19 gly deglycosylated 2064:2077 arg1 solECE-1 solECE-1 Cterm solECE-1 1889 Deglycosylation of solECE-1 by peptide N-glycosidase F shifted the apparent molecular weight of solECE-1 to approximately 80 kDa and the deglycosylated form(s) of solECE-1 preserved at least 72% of the activity of the glycosylated form. 9808768 13 96 gly Deglycosylation 1927:1941 arg1 solECE-1 solECE-1 Cterm solECE-1 1889 Deglycosylation of solECE-1 by peptide N-glycosidase F shifted the apparent molecular weight of solECE-1 to approximately 80 kDa and the deglycosylated form(s) of solECE-1 preserved at least 72% of the activity of the glycosylated form. 8243459 13 63 gly heterogeneity 1586:1598 arg1 gelatinase B gelatinase B PUBTATOR gelatinase B 17395 Regulation of activity and structural heterogeneity of gelatinase B in WEHI-3 cells were shown to occur at the gene regulatory level, by expression of the matrix metalloproteinase inhibitor TIMP-1, and by glycosylation of the secreted protein. 19193796 7 38 gly Sp1 1303:1305 arg1 O-GlcNAcylation Sp1 O-GlcNAcylation OGER Sp1 P08047 O-GlcNAcylation of the transcription factor Sp1 and the presence of Sp1-binding sites in the LTR were found to be crucial for this inhibitory effect. 16792699 5 76 gly N-glycosylation 712:726 arg1 wild-type Kv3.1 wild-type Kv3.1 PUBTATOR Kv3.1 25327 To demonstrate N-glycosylation of wild-type Kv3.1 in Sf9 cells, cells were treated with tunicamycin. 16809283 1 3 gly glycoprotein 171:182 arg1 gpUL37 gpUL37 Cterm gpUL37 3077462 The human cytomegalovirus (HCMV) UL37 glycoprotein (gpUL37) is internally cleaved and its products divergently traffic to mitochondria or are retained in the secretory pathway. 16809283 1 3 gly glycoprotein 171:182 arg1 The human cytomegalovirus (HCMV) UL37 glycoprotein The human cytomegalovirus (HCMV) UL37 glycoprotein PUBTATOR HCMV) UL37 glycoprotein 3077462 The human cytomegalovirus (HCMV) UL37 glycoprotein (gpUL37) is internally cleaved and its products divergently traffic to mitochondria or are retained in the secretory pathway. 8286855 9 49 gly glycosylation 1234:1246 arg1 GpA GpA PUBTATOR GpA 2993 These simple rules explain the glycosylation (or lack of it) on 21 of 22 Ser/Thr in the extracellular domain of GpA. 2708385 3 40 gly glycosylation 522:534 arg1 the hexosaminidase beta-chain the hexosaminidase beta-chain OGER chain P07686 The five potential glycosylation sites of the hexosaminidase beta-chain were modified individually by site-directed mutagenesis, and the constructs were expressed in COS 1 cells. 15866423 7 31 gly glycosylation 1104:1116 arg1 the LH receptor the LH receptor PUBTATOR LH receptor 25477 These results show that glycosylation of the LH receptor plays an important role in receptor processing and cell surface expression. 2608056 2 83 gly nonglycosylated 627:641 arg1 nonglycosylated LH beta nonglycosylated LH beta PUBTATOR LH beta 100689384 Pulsechase studies performed with stably transfected Chinese hamster ovary cells that expressed both alpha-subunit (fully glycosylated) and nonglycosylated LH beta revealed that turnover, transport, and secretion of newly synthesized, nonglycosylated LH beta were effectively blocked over a 22-h span. 10963791 4 59 gly underglycosylated 614:630 arg1 AT-N155Q AT Cterm AT Permanently transfected CHO cell lines were generated following transfection of the resulting constructs, encoding either the wild-type rabbit AT (AT-WT) or one of the four underglycosylated variants (AT-N96Q, AT-N135Q, AT-N155Q, and AT-N155Q). 10963791 4 59 gly underglycosylated 614:630 arg1 AT-N155Q AT Cterm AT Permanently transfected CHO cell lines were generated following transfection of the resulting constructs, encoding either the wild-type rabbit AT (AT-WT) or one of the four underglycosylated variants (AT-N96Q, AT-N135Q, AT-N155Q, and AT-N155Q). 10963791 4 59 gly underglycosylated 614:630 arg1 AT-N135Q AT Cterm AT Permanently transfected CHO cell lines were generated following transfection of the resulting constructs, encoding either the wild-type rabbit AT (AT-WT) or one of the four underglycosylated variants (AT-N96Q, AT-N135Q, AT-N155Q, and AT-N155Q). 10963791 4 59 gly underglycosylated 614:630 arg1 AT-N155Q AT Cterm AT Permanently transfected CHO cell lines were generated following transfection of the resulting constructs, encoding either the wild-type rabbit AT (AT-WT) or one of the four underglycosylated variants (AT-N96Q, AT-N135Q, AT-N155Q, and AT-N155Q). 10963791 4 59 gly underglycosylated 614:630 arg1 AT-N135Q AT Cterm AT Permanently transfected CHO cell lines were generated following transfection of the resulting constructs, encoding either the wild-type rabbit AT (AT-WT) or one of the four underglycosylated variants (AT-N96Q, AT-N135Q, AT-N155Q, and AT-N155Q). 10963791 4 59 gly underglycosylated 614:630 arg1 AT-N135Q AT Cterm AT Permanently transfected CHO cell lines were generated following transfection of the resulting constructs, encoding either the wild-type rabbit AT (AT-WT) or one of the four underglycosylated variants (AT-N96Q, AT-N135Q, AT-N155Q, and AT-N155Q). 15749002 7 39 gly glycosylation 1503:1515 arg1 its alpha subunits its alpha subunits OGER subunits P13674 The enzyme expressed in E. coli differed from those present in vivo and those produced in other hosts in that it lacked the N glycosylation of its alpha subunits, which may be advantageous in crystallization experiments. 16809283 8 63 gly N-glycosylated 1583:1596 arg1 gpUL37 gpUL37 Cterm gpUL37 3077462 Its NH(2)-terminal fragment, pUL37(M-NH2), was detected more abundantly in mitochondria, while its N-glycosylated C-terminal fragment, gpUL37(M-COOH), was detected predominantly in the ER in a manner analogous to that of gpUL37 cleavage products. 8750891 15 100 gly contain 2078:2084 arg1 5-HT2C receptors AND N-linked sugars 5-HT2C receptors N-linked sugars PUBTATOR 5-HT2C receptors 25187 These results demonstrate that 5-HT2C receptors contain N-linked sugars and suggest that sialic acid residues associate with 5-HT2C receptors in the choroid plexus. 28196864 4 20 part_of GP2 588:590 arg1 Asn563 GP2 Asn563 and Asn618 Cterm AminoAcid GP2 Asn563 and Asn618 As reported before, we found that, although GP1 NGSs are not critical, the two GP2 NGSs, Asn563 and Asn618, are essential for GP function. 9653080 1 4 gly sialoglycoprotein 149:165 arg1 Vascular adhesion protein 1 Vascular adhesion protein 1 OGER Vascular adhesion protein 1 Q16853 Vascular adhesion protein 1 (VAP-1) is a human endothelial sialoglycoprotein whose cell surface expression is induced under inflammatory conditions. 16388317 1 78 gly glycosylated 238:249 arg1 Carcinoembryonic antigen Carcinoembryonic antigen PUBTATOR Carcinoembryonic antigen 1084 Carcinoembryonic antigen (CEA), the most widely used human tumor marker, is a heavily glycosylated protein over-expressed by a wide range of tumors. 10713099 0 45 gly ligand-1 51:58 arg1 minimal determinants P-selectin glycoprotein ligand-1 minimal determinants PUBTATOR P-selectin glycoprotein ligand-1 6404 Noncovalent association of P-selectin glycoprotein ligand-1 and minimal determinants for binding to P-selectin. 10713099 0 68 gly glycoprotein 38:49 arg1 P-selectin glycoprotein ligand-1 P-selectin glycoprotein ligand-1 PUBTATOR P-selectin glycoprotein ligand-1 6404 Noncovalent association of P-selectin glycoprotein ligand-1 and minimal determinants for binding to P-selectin. 17176047 7 33 gly glycoforms 1149:1158 arg1 MMP-9 MMP-9 PUBTATOR MMP-9 4318 Consistent with the glycan analysis, surface plasmon resonance binding assays indicated that the cancer-associated glycoforms of MMP-9 bound galectin-3 with an affinity and avidity significantly reduced compared with those of the natural neutrophil MMP-9. 24361716 1 50 gly glycosylation 271:283 arg1 ADAM17 ADAM17 PUBTATOR ADAM17 6868 ADAM17 (a disintegrin and metalloprotease 17) is believed to be a tractable target in various diseases, including cancer and rheumatoid arthritis; however, it is not known whether glycosylation of ADAM17 expressed in healthy cells differs from that found in diseased tissue and, if so, whether glycosylation affects inhibitor binding. 17158864 0 54 gly glycosylation 38:50 arg1 proteinase 3 proteinase 3 PUBTATOR proteinase 3 5657 Functional significance of Asn-linked glycosylation of proteinase 3 for enzymatic activity, processing, targeting, and recognition by anti-neutrophil cytoplasmic antibodies. 25499076 5 45 gly present 859:865 arg1 native, pituitary G-hPRL AND the N-glycan structures native, pituitary G-hPRL the N-glycan structures PUBTATOR hPRL 5617 The main objective of this study was to determine the N-glycan structures present in native, pituitary G-hPRL and compare them with those present in the recombinant hormone. 24113653 2 3 gly modified 289:296 arg1 Tau AND O-linked N-acetylglucosamine Tau O-linked N-acetylglucosamine OGER Tau P10636 Tau is posttranslationally modified by O-linked N-acetylglucosamine (O-GlcNAc), and increasing tau O-GlcNAcylation may protect against its aggregation. 24113653 2 3 gly modified 289:296 arg3 Tau AND O-GlcNAc Tau O-GlcNAc OGER Tau P10636 Tau is posttranslationally modified by O-linked N-acetylglucosamine (O-GlcNAc), and increasing tau O-GlcNAcylation may protect against its aggregation. 19004834 6 74 gly O-glycosylation 997:1011 arg1 CD44 CD44 PUBTATOR CD44 960 Use of highly specific enzymes and metabolic inhibitors reveals that LS174T CD44 binding to fibrin is dependent on O-glycosylation of CD44, whereas CD44s-fibrin(ogen) interaction has an absolute requirement for N-, but not O-, linked glycans. 1352293 0 67 gly N-glycosylation 34:48 arg1 A. A. PUBTATOR arylsulfatase A 410 In vitro mutagenesis of potential N-glycosylation sites of arylsulfatase A. Effects on glycosylation, phosphorylation, and intracellular sorting. 22164239 11 136 gly sialylated 1595:1604 arg1 VL VL Cterm VL Interestingly, highly sialylated spectrin(VL) showed decreased binding with spectrin-depleted inside-out membrane vesicles of normal erythrocytes compared to spectrin(N) suggesting functional abnormality. 7507481 5 4 gly glycosylated 511:522 arg1 CHIP CHIP OGER CHIP Q9UNE7 CHIP is glycosylated at Asn-42, indicating loop A is exofacial. 22678432 7 8 gly IgG 1212:1214 arg1 released glycans IgG released glycans Cterm IgG The data obtained from analyzing released glycans of rHuEPO and IgG, described in the second protocol of this series (10.1038/nprot.2012.063), provide complementary detailed glycan structural information that facilitates characterization of the glycopeptides. 22678432 7 14 gly rHuEPO 1201:1206 arg1 released glycans rHuEPO released glycans Cterm rHuEPO 2056 The data obtained from analyzing released glycans of rHuEPO and IgG, described in the second protocol of this series (10.1038/nprot.2012.063), provide complementary detailed glycan structural information that facilitates characterization of the glycopeptides. 28322444 1 13 gly glycosylated 198:209 arg1 The potassium ion channel Kv3.1b The potassium ion channel Kv3.1b Cterm Kv3.1b The potassium ion channel Kv3.1b is a member of a family of voltage-gated ion channels that are glycosylated in their mature form. 7589110 1 46 gly glycoprotein 131:142 arg1 E-selectin E-selectin PUBTATOR E-selectin 6401 E-selectin is a cytokine-inducible membrane glycoprotein capable of mediating adhesion of leukocytes to endothelial cells. 18191143 5 101 part_of Ser198 588:593 arg1 LPO LPO Ser198 PUBTATOR AminoAcid LPO 4025 Ser198 The structure determination revealed an unexpected phosphorylation of Ser198 in LPO, which is also confirmed by anti-phosphoserine antibody binding studies. 9442070 13 46 gly Fc 2176:2177 arg1 sialylation IgA1 Fc sialylation OGER IgA1 Fc P01876 The accessibility of the Cα 2 N-glycans provides an explanation for the increased sialylation and galactosylation of IgA1 Fc over that of IgG Fc N-glycans, which are confined in the space between the two Cγ 2 domains. 9442070 13 93 gly sialylation 2136:2146 arg1 IgA1 Fc IgA1 Fc OGER IgA1 Fc P01876 The accessibility of the Cα 2 N-glycans provides an explanation for the increased sialylation and galactosylation of IgA1 Fc over that of IgG Fc N-glycans, which are confined in the space between the two Cγ 2 domains. 23894062 8 94 part_of NOG 1130:1132 arg1 NOG-N54 NOG NOG-N54 PUBTATOR SpecificSite NOG 9241 N54 We showed a SOST peptide (SOST-S146, with homology to a bacterial glycotransferase peptide) binds to a NOG peptide (NOG-N54), which contains a N-glycosylation site. 27038031 4 20 gly N-glycosylation 476:490 arg1 recombinant pentameric and hexameric IgM recombinant pentameric and hexameric IgM OGER IgM P01871 In this study, the N-glycosylation of recombinant pentameric and hexameric IgM produced by the same human cell type and culture conditions was site-specifically profiled by RP-LC-CID/ETD-MS/MS using HILIC-enriched tryptic and GluC glycopeptides. 12354382 0 19 gly modification 37:48 arg3 PSGL-1 AND a novel carbohydrate modification PSGL-1 a novel carbohydrate modification PUBTATOR PSGL-1 6404 6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells. 12354382 0 50 gly PSGL-1 53:58 arg1 a novel carbohydrate modification PSGL-1 a novel carbohydrate modification PUBTATOR PSGL-1 6404 6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells. 12354382 0 50 gly PSGL-1 53:58 arg1 6-Sulfo LacNAc PSGL-1 6-Sulfo LacNAc PUBTATOR PSGL-1 6404 6-Sulfo LacNAc, a novel carbohydrate modification of PSGL-1, defines an inflammatory type of human dendritic cells. 2041080 6 13 gly HA1 1200:1202 arg1 the carbohydrate HA1 the carbohydrate Cterm HA1 The observation that these mutants were not cleaved, even when the carbohydrate at asparagine 22 of HA1 was absent, underscores the fact that the basic peptide had to be generated by insertion to obtain cleavage. 23661698 9 52 gly glycosylation 1255:1267 arg1 ephrin-A1 ephrin-A1 PUBTATOR ephrin-A1 1942 These findings suggest that the glycosylation on ephrin-A1 plays a critical role in the binding and activation of the EphA2 receptor. 2033065 0 110 gly Glycosylation 0:12 arg1 human protein C human protein C OGER protein C P02810 Glycosylation of human protein C affects its secretion, processing, functional activities, and activation by thrombin. 25499264 2 52 gly gp120 440:444 arg1 multiple N-linked glycans envelope glycoprotein gp120 multiple N-linked glycans PUBTATOR envelope glycoprotein gp120 100616444 The development of phenotypic resistance to CBAs by the virus is accompanied by the deletion of multiple N-linked glycans of the surface envelope glycoprotein gp120. 25499264 2 128 gly glycoprotein 427:438 arg1 the surface envelope glycoprotein gp120 the surface envelope glycoprotein gp120 PUBTATOR envelope glycoprotein gp120 100616444 The development of phenotypic resistance to CBAs by the virus is accompanied by the deletion of multiple N-linked glycans of the surface envelope glycoprotein gp120. 8764057 5 10 gly glycosylation 842:854 arg1 gC2 gC2 PUBTATOR gC2 83733 The molecular weight and extent of glycosylation of gC1 (457t), gC1(delta33-123t), and gC2(426t) were determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometric analysis. 8764057 5 10 gly glycosylation 842:854 arg1 delta33-123t delta33-123t Cterm delta33-123t 79751 The molecular weight and extent of glycosylation of gC1 (457t), gC1(delta33-123t), and gC2(426t) were determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometric analysis. 8764057 5 10 gly glycosylation 842:854 arg1 426t 426t Cterm 426t 83733 The molecular weight and extent of glycosylation of gC1 (457t), gC1(delta33-123t), and gC2(426t) were determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometric analysis. 8764057 5 10 gly glycosylation 842:854 arg1 457t 457t Cterm 457t 79751 The molecular weight and extent of glycosylation of gC1 (457t), gC1(delta33-123t), and gC2(426t) were determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometric analysis. 8764057 5 10 gly glycosylation 842:854 arg1 gC1 gC1 PUBTATOR gC1 79751 The molecular weight and extent of glycosylation of gC1 (457t), gC1(delta33-123t), and gC2(426t) were determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometric analysis. 12023968 7 67 gly IgD 1243:1245 arg1 incomplete hinge carbohydrates IgD incomplete hinge carbohydrates OGER IgD P01880 Using benzyl 2-acetamido-2-deoxy-alpha-d-galactopyranoside (BADG) to inhibit complete O-linked glycosylation, we found that IgA1 and IgD with incomplete hinge carbohydrates were assembled and secreted from cells. 12023968 7 72 gly IgA1 1234:1237 arg1 incomplete hinge carbohydrates IgA1 incomplete hinge carbohydrates PUBTATOR IgA1 3493 Using benzyl 2-acetamido-2-deoxy-alpha-d-galactopyranoside (BADG) to inhibit complete O-linked glycosylation, we found that IgA1 and IgD with incomplete hinge carbohydrates were assembled and secreted from cells. 8206884 1 38 gly glycosylated 117:128 arg1 human interleukin 6 human interleukin 6 OGER interleukin 6 P05231 A glycosylated form of human interleukin 6 (hIL-6) has been produced in Chinese hamster ovary (CHO) cells transfected with a cDNA clone for human IL-6. 8206884 1 38 gly glycosylated 117:128 arg1 hIL-6 hIL-6 PUBTATOR hIL-6 3569 A glycosylated form of human interleukin 6 (hIL-6) has been produced in Chinese hamster ovary (CHO) cells transfected with a cDNA clone for human IL-6. 8323299 5 71 gly glycosylation 697:709 arg1 lamp-2 lamp-2 PUBTATOR In lamp-2 3920 In lamp-2, complete glycosylation was found at Ser-167, Thr-168, Thr-172, Thr-175, Thr-176, Thr-182, and Thr-183, and partial glycosylation at Ser-179 and Thr-181, and possibly also at Thr-185. 30127001 3 42 gly NOTCH1 497:502 arg1 Epidermal Growth Factor-like (EGF) repeat 11 NOTCH1 Epidermal Growth Factor-like (EGF) repeat 11 PUBTATOR NOTCH1 4851 This serine occurs between conserved cysteines 3 and 4 of Epidermal Growth Factor-like (EGF) repeat 11 of NOTCH1, a site distinct from those modified by protein O-glucosyltransferase 1 (POGLUT1), suggesting that a different enzyme is responsible. 7864354 0 66 gly glycoforms 65:74 arg1 recombinant human interferon-gamma glycoforms recombinant human interferon-gamma glycoforms PUBTATOR interferon-gamma 3458 High-resolution separation of recombinant human interferon-gamma glycoforms by micellar electrokinetic capillary chromatography. 15886209 6 22 gly glycosylation 906:918 arg1 factor VIII factor VIII OGER factor VIII P00451 We also present evidence that this interaction is independent of the glycosylation state of factor VIII but requires native calcium concentration in the endoplasmic reticulum. 10207016 2 49 gly N-glycosylated 340:353 arg1 the M protein the M protein OGER M protein P54296 The pre-S2 domain, present only in M and L proteins, is further N-glycosylated at Asn-4 exclusively in the M protein. 9696834 1 58 gly glycoprotein 201:212 arg1 human cytomegalovirus (CMV) glycoprotein B human cytomegalovirus (CMV) glycoprotein B Cterm human cytomegalovirus (CMV) glycoprotein B We previously reported that human cytomegalovirus (CMV) glycoprotein B (gB) is vectorially transported to apical membranes of CMV-infected polarized human retinal pigment epithelial cells propagated on permeable filter supports and that virions egress predominantly from the apical membrane domain. 2016314 0 83 gly glycosylation 56:68 arg1 rat renal renin rat renal renin PUBTATOR renin 24715 Amino-terminal amino acid sequence and heterogeneity in glycosylation of rat renal renin. 18695951 1 8 gly glycosylation 206:218 arg1 recombinant coagulation factor VIIa recombinant coagulation factor VIIa Cterm factor VIIa The two asparagine-linked glycosylation sites of recombinant coagulation factor VIIa have been characterized by glycosidase digestions, size-exclusion chromatography (SEC), and mass spectrometry (MS). 21172408 1 3 gly glycoprotein 179:190 arg1 Tissue factor Tissue factor PUBTATOR Tissue factor 2152 BACKGROUND: Tissue factor (TF) is a single polypeptide integral membrane glycoprotein composed of 263 residues and is essential to life in its role as the initiator of blood coagulation. 27604319 1 35 gly glycoprotein 148:159 arg1 SU SU Cterm SU Q14624 The surface envelope glycoprotein (SU) of Human immunodeficiency virus type 1 (HIV-1), gp120(SU) plays an essential role in virus binding to target CD4+ T-cells and is a major vaccine target. 27604319 1 35 gly glycoprotein 148:159 arg1 The surface envelope glycoprotein The surface envelope glycoprotein PUBTATOR envelope glycoprotein 155971 The surface envelope glycoprotein (SU) of Human immunodeficiency virus type 1 (HIV-1), gp120(SU) plays an essential role in virus binding to target CD4+ T-cells and is a major vaccine target. 25789582 2 27 gly TARG1 422:426 arg1 PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose TARG1 PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose PUBTATOR TARG1 221443 Several cellular enzymes, such as macrodomain containing proteins PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose (ADPr) protein glycohydrolase], reverse protein ADP-ribosylation. 25789582 2 51 gly PARG 379:382 arg1 ADP-ribose PARG ADP-ribose PUBTATOR PARG 8505 Several cellular enzymes, such as macrodomain containing proteins PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose (ADPr) protein glycohydrolase], reverse protein ADP-ribosylation. 25789582 2 51 gly PARG 379:382 arg1 PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose PARG PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose PUBTATOR PARG 8505 Several cellular enzymes, such as macrodomain containing proteins PARG [poly(ADP-ribose) glycohydrolase] and TARG1 [terminal ADP-ribose (ADPr) protein glycohydrolase], reverse protein ADP-ribosylation. 20512925 10 57 gly N-glycans 1432:1440 arg1 RAGE RAGE N-glycans PUBTATOR RAGE 177 These results demonstrate that carboxylated N-glycans on RAGE enhance binding potential and promote receptor clustering and subsequent signaling events following oligomeric S100A12 binding. 9254044 1 23 gly glycoprotein 213:224 arg1 Glucosidase II Glucosidase II OGER Glucosidase II Glucosidase II is an ER resident glycoprotein involved in the processing of N-linked glycans and probably a component of the ER quality control of glycoproteins. 2605214 1 44 gly Lol 227:229 arg1 a Lolium perenne (rye grass) pollen allergen Lol p III a Lolium perenne (rye grass) pollen allergen OGER Lol p III Q08397 The complete amino acid sequence of a Lolium perenne (rye grass) pollen allergen, Lol p III, determined by the automated Edman degradation of the protein and its selected fragments, is reported in this paper. 26336134 7 46 gly glycosylation 1007:1019 arg1 HRG HRG PUBTATOR HRG 3273 The glycosylation of HRG may play a key competitive role in the interaction between HRG and heparin sulfate for binding bFGF and activating the FGF receptor. 15280465 3 17 gly SP-A 672:675 arg1 the carbohydrate recognition domain SP-A the carbohydrate recognition domain PUBTATOR SP-A 20387 Previous studies have shown that binding of SP-D to IAV is dependent on the glycosylation of specific sites on the HA1 domain of hemagglutinin on the surface of IAV, while the binding of SP-A to the HA1 domain is dependent on the glycosylation of the carbohydrate recognition domain of SP-A. 15280465 3 74 gly glycosylation 616:628 arg1 SP-A SP-A PUBTATOR SP-A 20387 Previous studies have shown that binding of SP-D to IAV is dependent on the glycosylation of specific sites on the HA1 domain of hemagglutinin on the surface of IAV, while the binding of SP-A to the HA1 domain is dependent on the glycosylation of the carbohydrate recognition domain of SP-A. 12637583 6 60 gly N-glycans 1018:1026 arg1 the H chains chains N-glycans OGER chains P40306 We also show that the N-glycans on the H chains of both SIgA1 and SIgA2 present terminal GlcNAc and mannose residues that are normally masked by SC, but that can be unmasked and recognized by mannose-binding lectin, by disrupting the SC-H chain noncovalent interactions. 17041212 6 7 part_of protein 1157:1163 arg1 aa residues 363 to 368 S protein aa residues 363 to 368 OGER SpecificSite S protein Q15517 residues 363 Data from a pepscan analysis and M13 phage peptide display library system mapped the reactive MAb SIa5 epitope to aa residues 363 to 368 of the S protein. 11981562 5 32 gly glycosylation 759:771 arg1 natural hLF natural hLF PUBTATOR hLF 3131 Although natural hLF and rhLF underwent differential N-linked glycosylation, they were equally effective in three different in vivo infection models employing immunocompetent and leukocytopenic mice, and showed similar localization at sites of infection. 11981562 5 32 gly glycosylation 759:771 arg1 rhLF rhLF OGER rhLF P02788 Although natural hLF and rhLF underwent differential N-linked glycosylation, they were equally effective in three different in vivo infection models employing immunocompetent and leukocytopenic mice, and showed similar localization at sites of infection. 11209750 7 69 gly unglycosylated 1112:1125 arg1 unglycosylated kappaNS1 chains unglycosylated kappaNS1 chains OGER kappaNS1 chains Q9R1P4 None of these drugs, except lactacystin, affected the degradation of unglycosylated kappaNS1 chains. 9362042 5 2 gly N-glycosylation 794:808 arg1 N8 NA N8 NA Cterm N8 NA These observations indicate that N-glycosylation at Asn 144 of N8 NA may be conserved from the functional requirement, but not from the structural necessity. 29944110 2 37 gly glycoprotein 346:357 arg1 HA HA Cterm HA The impact can be reduced by vaccination, which induces neutralizing antibodies that mainly target the haemagglutinin glycoprotein (HA). 24573035 5 14 gly N-glycosylation 1049:1063 arg1 CRISP3 CRISP3 PUBTATOR CRISP3 10321 Validated protein was used in comparative structure/function studies to characterise sites and patterns of N-glycosylation in CRISP3, revealing interesting inter-species differences. 3965499 9 15 gly glycosylation 1696:1708 arg1 colorectal mucin colorectal mucin PUBTATOR mucin 100508689 These results demonstrate that the expression of lectin-binding sites in human large intestinal goblet mucin is specifically altered in inflammatory bowel disease, indicating that there are changes in glycosylation of colorectal mucin consistent with alterations in goblet cell differentiation. 21045010 8 108 gly GAT1 1383:1386 arg1 terminal sialic acids GAT1 terminal sialic acids OGER GAT1 P30531 However, deficiency and removal of terminal sialic acids of GAT1 reduced the V(max) GABA values with a reduced apparent affinity for extracellular Na(+). 2456913 8 0 part_of beta-subunits 1077:1089 arg1 Asn23 TSH beta Asn23 PUBTATOR AminoAcid TSH beta 7252 Asn23 The processing at Asn23 of TSH beta-subunits was slower than that at Asn56 or Asn82 of alpha-subunits. 10189832 10 162 gly IgA1 1754:1757 arg1 residual carbohydrate content IgA1 residual carbohydrate content PUBTATOR IgA1 3493 Quantitative determination of residual carbohydrate content of IgA1 after incubation with bacterial cells of Gram-positive rods has confirmed that they remove sialic acid, and in addition to that, only minor amounts of carbohydrates. 1482344 0 39 gly glycosylated 20:31 arg1 glycosylated human butyrylcholinesterase glycosylated human butyrylcholinesterase PUBTATOR butyrylcholinesterase 590 A computer model of glycosylated human butyrylcholinesterase. 22159084 13 78 gly N-glycosylation 1706:1720 arg1 SLC26A3 SLC26A3 PUBTATOR SLC26A3 1811 In conclusion, our data indicate that N-glycosylation of SLC26A3 is important for cell surface expression and for protection from proteolytic degradation that may contribute to the understanding of pathogenesis of congenital disorders of glycosylation. 11827520 0 24 gly glycosylated 67:78 arg1 glycosylated full-length HIVgp120 glycosylated full-length HIVgp120 Cterm HIVgp120 3700 Characterization of the tertiary structure of soluble CD4 bound to glycosylated full-length HIVgp120 by chemical modification of arginine residues and mass spectrometric analysis. 26742847 9 6 gly N-glycosylation 1793:1807 arg1 CaVα2δ1 CaVα2δ1 Cterm CaVα2δ1 These results demonstrate that Asn-663 and to a lesser extent Asn-348, Asn-468, and Asn-812 contribute to protein stability/synthesis of CaVα2δ1, and furthermore that N-glycosylation of CaVα2δ1 is essential to produce functional L-type Ca(2+) channels. 23723439 4 12 gly glycosylation 998:1010 arg1 α-DG α-DG Cterm DG Q14118 We previously reported that human natural killer-1 sulfotransferase (HNK-1ST), which was originally reported as one of the enzymes responsible for HNK-1 glycoepitope, had an ability to suppress the glycosylation and the function of α-DG. 15869464 1 85 gly glycoprotein 258:269 arg1 OPN OPN PUBTATOR OPN 6696 OPN (osteopontin) is an integrin-binding highly phosphorylated glycoprotein, recognized as a key molecule in a multitude of biological processes such as bone mineralization, cancer metastasis, cell-mediated immune response, inflammation and cell survival. 20512925 6 6 gly modified 816:823 arg1 RAGE AND complex glycans RAGE complex glycans PUBTATOR RAGE 177 When expressed in mammalian cells, RAGE is modified by complex glycans predominantly at the first glycosylation site (N25IT) that retains S100A12 binding. 15448157 13 9 gly diglycosylation 1878:1892 arg1 C C Cterm C 19122 These data indicate that diglycosylation of PrP(C) is not mandatory for prion infection in vivo. 15448157 13 9 gly diglycosylation 1878:1892 arg1 PrP PrP PUBTATOR PrP 19122 These data indicate that diglycosylation of PrP(C) is not mandatory for prion infection in vivo. 26634432 3 19 gly N-terminal 308:317 arg1 a new proteoglycan N-terminal a new proteoglycan Cterm N-terminal After glycosylation, an N-terminal fragment of DMP1 protein was identified as a new proteoglycan (DMP1-PG) in bone matrix. 26634432 3 56 gly protein 336:342 arg1 a new proteoglycan DMP1 protein a new proteoglycan PUBTATOR DMP1 protein 13406 After glycosylation, an N-terminal fragment of DMP1 protein was identified as a new proteoglycan (DMP1-PG) in bone matrix. 22023369 3 40 gly sFcγRIIIa 568:576 arg1 the two N-glycans FcγRIIIa the two N-glycans PUBTATOR FcγRIIIa 2214 The crystal structure shows that one of the two N-glycans of sFcγRIIIa mediates the interaction with nonfucosylated Fc, thereby stabilizing the complex. 8457384 3 50 gly Deglycosylated 379:392 arg1 Deglycosylated gp120 Deglycosylated gp120 PUBTATOR Deglycosylated gp120 3700 Deglycosylated gp120 binds to GalCer, suggesting that the amino acids of glycoprotein gp120 and not the carbohydrates are responsible for the observed binding. 1671337 6 71 gly sites 941:945 arg1 human protein S human protein S sites Cterm human protein S Exon XIII contains all three potential N-glycosylation sites in human protein S. 30052682 4 1 gly N-glycosylated 838:851 arg1 N-glycosylated gB N-glycosylated gB Cterm N-glycosylated gB 79594 Here, we report the identification of F-box only protein 2 (FBXO2), an SCF ubiquitin ligase substrate adaptor that preferentially binds high-mannose glycans and attenuates EBV infectivity by targeting N-glycosylated gB for degradation. 1897978 2 34 gly glycosylated 420:431 arg1 Namalwa-derived hGM-CSF Namalwa-derived hGM-CSF PUBTATOR Namalwa-derived hGM-CSF 1437 From the results of tunicamycin treatment and N-glycosidase F digestion, it was demonstrated that Namalwa-derived hGM-CSF was highly glycosylated at two potential N-glycosylation sites and several O-glycosylation sites as previously shown for naturally occurring hGM-CSF. 20497179 1 11 gly glycosylated 84:95 arg1 CD34 CD34 PUBTATOR CD34 12490 CD34 is a highly glycosylated sialomucin expressed on a variety of cells, ranging from vascular endothelial cells to haematopoietic stem cells. 7615513 0 4 gly molecule 78:85 arg1 specific polysialylation neural cell adhesion molecule specific polysialylation PUBTATOR neural cell adhesion molecule 428253 Protein determinants for specific polysialylation of the neural cell adhesion molecule. 7615513 0 64 gly polysialylation 34:48 arg1 the neural cell adhesion molecule the neural cell adhesion molecule PUBTATOR neural cell adhesion molecule 428253 Protein determinants for specific polysialylation of the neural cell adhesion molecule. 12637792 10 80 gly deglycosylated 1807:1820 arg1 deglycosylated hCG deglycosylated hCG PUBTATOR hCG 93659 CONCLUSIONS: We conclude that in contrast to deglycosylated hCG, all hCG glycosylation isoforms investigated act as receptor agonists. 17956937 0 46 gly antigen 50:56 arg1 Oligosaccharide profiles prostate specific antigen Oligosaccharide profiles OGER prostate specific antigen P07288 Oligosaccharide profiles of the prostate specific antigen in free and complexed forms from the prostate cancer patient serum and in seminal plasma: a glycopeptide approach. 19818407 1 55 gly glycoprotein 181:192 arg1 Bovine CD38 Bovine CD38 PUBTATOR Bovine CD38 327677 Bovine CD38, a type II glycoprotein, contains two potential N-glycosylation sites (Asn-201 and Asn-268) in its extracellular domain. 9063619 6 15 gly glycosylation 1002:1014 arg1 IgG IgG Cterm IgG Examples include protein-specific glycosylation within the immunoglobulins and immunoglobulin superfamily and site-specific processing in ribonuclease, Thy-1, IgG, tissue plasminogen activator, and influenza A hemagglutinin. 9063619 6 15 gly glycosylation 1002:1014 arg1 Thy-1 Thy-1 PUBTATOR Thy-1 7070 Examples include protein-specific glycosylation within the immunoglobulins and immunoglobulin superfamily and site-specific processing in ribonuclease, Thy-1, IgG, tissue plasminogen activator, and influenza A hemagglutinin. 23389048 1 13 gly fucosylated 239:249 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Development of liver disease is associated with the appearance of multiply fucosylated glycoforms of haptoglobin. 23389048 1 68 gly glycoforms 251:260 arg1 haptoglobin haptoglobin PUBTATOR haptoglobin 3240 Development of liver disease is associated with the appearance of multiply fucosylated glycoforms of haptoglobin. 23389048 1 115 gly haptoglobin 265:275 arg1 multiply fucosylated glycoforms haptoglobin multiply fucosylated glycoforms PUBTATOR haptoglobin 3240 Development of liver disease is associated with the appearance of multiply fucosylated glycoforms of haptoglobin. 15140192 5 20 gly glycosylation 708:720 arg1 wild-type prestin wild-type prestin PUBTATOR prestin 375611 Further, treatment with tunicamycin or glycopeptidase-F was used to determine the consequences of removing N-linked glycosylation in wild-type prestin. 26348848 11 111 gly N-glycosylation 2012:2026 arg1 Kv3.1a Kv3.1a PUBTATOR Kv3 29731 Our study demonstrates that N-glycosylation of Kv3.1a, like Kv3.1b, provides a mechanism for the distribution of these proteins to the cell body and outgrowths and thereby can generate different voltage-dependent conductances in these membranes. 20408681 6 44 gly attached 800:807 arg2 hLF AND the three glycan chains hLF the three glycan chains PUBTATOR hLF 3131 In comparison, each of the three glycan chains attached to hLF contains 12-13 sugar groups and is twice as long. 22781125 1 1 gly glycosylation 326:338 arg1 α-DG α-DG Cterm α-DG Defects in the O-linked glycosylation of the peripheral membrane protein α-dystroglycan (α-DG) are the main cause of several forms of congenital muscular dystrophies and thus the characterization of the glycosylation of α-DG is of great medical importance. 22781125 1 36 gly glycosylation 147:159 arg1 α-DG α-DG Cterm α-DG Defects in the O-linked glycosylation of the peripheral membrane protein α-dystroglycan (α-DG) are the main cause of several forms of congenital muscular dystrophies and thus the characterization of the glycosylation of α-DG is of great medical importance. 27314333 4 12 gly N-glycosylated 695:708 arg1 Rspo3 Rspo3 PUBTATOR Rspo3 84870 Using chemical inhibitors, deglycosylase and site-directed mutagenesis, we found that human Rspo1 and Rspo3 are both N-glycosylated at N137, a site near the C-terminus of the furin repeat 2 domain, and Rspo2 is N-glycosylated at N160, a position near the N-terminus of TSR1 domain. 27314333 4 12 gly N-glycosylated 695:708 arg1 human Rspo1 human Rspo1 PUBTATOR Rspo1 284654 Using chemical inhibitors, deglycosylase and site-directed mutagenesis, we found that human Rspo1 and Rspo3 are both N-glycosylated at N137, a site near the C-terminus of the furin repeat 2 domain, and Rspo2 is N-glycosylated at N160, a position near the N-terminus of TSR1 domain. 27314333 4 50 gly N-glycosylated 789:802 arg1 Rspo2 Rspo2 PUBTATOR Rspo2 340419 Using chemical inhibitors, deglycosylase and site-directed mutagenesis, we found that human Rspo1 and Rspo3 are both N-glycosylated at N137, a site near the C-terminus of the furin repeat 2 domain, and Rspo2 is N-glycosylated at N160, a position near the N-terminus of TSR1 domain. 2477227 6 42 gly presence 824:831 arg2 hCG AND two N-linked oligosaccharides hCG two N-linked oligosaccharides PUBTATOR hCG beta 1082 The amounts of sugar that were found relative to peptide indicated the presence of two N-linked oligosaccharides per molecule on both beta-core and hCG beta. 11275255 5 55 gly glycoforms 794:803 arg1 IgG-Fc IgG-Fc Cterm IgG We report the generation of a series of truncated glycoforms of IgG-Fc, and the analysis of the contribution of the residual oligosaccharide to IgG-Fc function and thermal stability. 11874698 12 38 gly hyperglycosylated 1927:1943 arg1 C5 C5 Cterm C5 Antibody B152 was produced using a choriocarcinoma-derived hCG (C5), which was hyperglycosylated at both N- and O-linked sites and was 100% nicked at position beta(47-48). 11874698 12 38 gly hyperglycosylated 1927:1943 arg1 a choriocarcinoma-derived hCG a choriocarcinoma-derived hCG OGER hCG Antibody B152 was produced using a choriocarcinoma-derived hCG (C5), which was hyperglycosylated at both N- and O-linked sites and was 100% nicked at position beta(47-48). 9443901 7 46 gly Glycosylation 945:957 arg1 gp120 gp120 PUBTATOR gp120 3700 Glycosylation of gp120 is critical for its uptake by adsorptive endocytosis since the non-glycosylated form of gp120 is unaffected by wheatgerm agglutinin. 9443901 7 49 gly non-glycosylated 1031:1046 arg1 the non-glycosylated form form of gp120 PUBTATOR form of gp120 3700 Glycosylation of gp120 is critical for its uptake by adsorptive endocytosis since the non-glycosylated form of gp120 is unaffected by wheatgerm agglutinin. 1333104 5 22 gly nonglycosylated 997:1011 arg1 nonglycosylated t-PA nonglycosylated t-PA PUBTATOR t-PA 5327 Using this test system binding of both recombinant glycosylated human t-PA produced in Chinese hamster ovary cells (CHO-t-PA) and of nonglycosylated t-PA, produced in E. coli (BM 06.021) was investigated. 1333104 5 78 gly glycosylated 915:926 arg1 both recombinant glycosylated human t-PA both recombinant glycosylated human t-PA PUBTATOR t-PA 5327 Using this test system binding of both recombinant glycosylated human t-PA produced in Chinese hamster ovary cells (CHO-t-PA) and of nonglycosylated t-PA, produced in E. coli (BM 06.021) was investigated. 10029548 0 68 gly glycosylated 144:155 arg1 human transferrin human transferrin PUBTATOR transferrin 7018 X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32. 26088564 10 44 gly fucosylation 1408:1419 arg1 AGP structure AGP structure Cterm AGP By examining the influence of fucosylation on AGP structure and binding to selectins, it is proposed that the latter may bind to glycan chains linked to Asn54 and Asn75, and that this binding may involve other glycans, such as the one attached to Asn15. 19522481 6 0 gly glycoprotein 1221:1232 arg1 lysosome associated membrane glycoprotein 1 lysosome associated membrane glycoprotein 1 PUBTATOR lysosome associated membrane glycoprotein 1 3916 However, only three N-linked glycosylated proteins, galectin-3 binding protein, lysosome associated membrane glycoprotein 1, and oxygen regulated protein, were identified in all three breast cancer cell lines. 19522481 6 92 gly glycosylated 1141:1152 arg1 lysosome associated membrane glycoprotein 1 lysosome associated membrane glycoprotein 1 PUBTATOR lysosome associated membrane glycoprotein 1 3916 However, only three N-linked glycosylated proteins, galectin-3 binding protein, lysosome associated membrane glycoprotein 1, and oxygen regulated protein, were identified in all three breast cancer cell lines. 19522481 6 92 gly glycosylated 1141:1152 arg1 galectin-3 binding protein galectin-3 binding protein PUBTATOR galectin-3 binding protein 3959 However, only three N-linked glycosylated proteins, galectin-3 binding protein, lysosome associated membrane glycoprotein 1, and oxygen regulated protein, were identified in all three breast cancer cell lines. 10191272 6 74 gly N-glycosylation 1580:1594 arg1 the AT1 receptor expression the AT1 receptor expression Cterm AT1 These results demonstrate that N-glycosylation is required for the AT1 receptor expression. 7681247 0 9 gly glycoprotein 149:160 arg1 human alpha 2-HS glycoprotein human alpha 2-HS glycoprotein PUBTATOR alpha 2-HS glycoprotein 197 Molecular cloning and sequence analysis of cDNA for a 59 kD bone sialoprotein of the rat: demonstration that it is a counterpart of human alpha 2-HS glycoprotein and bovine fetuin. 10743795 1 29 gly glycoprotein 217:228 arg1 lubricin lubricin PUBTATOR lubricin 10216 OBJECTIVE: The boundary lubricating ability of human synovial fluid has been attributed to lubricin, a mucinous glycoprotein. 23754285 6 25 gly glycosylated 772:783 arg1 ADAMDEC1 ADAMDEC1 PUBTATOR Thus, ADAMDEC1 27299 Thus, ADAMDEC1 is secreted as a mature, glycosylated, and proteolytically active metalloprotease, capable of cleaving macromolecular substrates. 16125194 6 8 gly non-glycosylated 1250:1265 arg1 The non-glycosylated recombinant cathepsin B The non-glycosylated recombinant cathepsin B OGER cathepsin B P07858 The non-glycosylated recombinant cathepsin B migrated as a single band of 39 kDa on SDS-PAGE. 22688517 5 67 gly LOX-1 995:999 arg1 N-glycans structures LOX-1 N-glycans structures PUBTATOR LOX-1 4973 Here, an approach using nonspecific protease (Pronase E) digestion followed by MALDI-QIT-TOF MS and multistage MS (MS(3)) analysis is explored to obtain site-specific N-glycosylation information of recombinant human LOX-1, in combination with glycan structure confirmation through characterizing released glycans using tandem MS. The results reveal that N-glycans structures as well as their corresponding attached site of LOX-1 can be identified simultaneously by direct MS analysis of glycopeptides from non-specific protease digestion. 25482829 1 5 gly glycoprotein 92:103 arg1 Tenascin-C Tenascin-C PUBTATOR Tenascin-C 3371 Tenascin-C is a large, multimodular, extracellular matrix glycoprotein that exhibits a very restricted pattern of expression but an enormously diverse range of functions. 17500062 9 57 gly glycosylated 1482:1493 arg1 ObOPN ObOPN PUBTATOR OPN 20750 These residues are fully modified in FbOPN, whereas one site is partially glycosylated in ObOPN. 19420742 4 36 gly alpha2,6 817:824 arg1 ST6GalI alpha2,6 ST6GalI Cterm alpha2,6 Here, we focus mainly on the modification of N-glycans with N-acetylglucosaminyltransferase III (GnT-III), N-acetylglucosaminyltransferase V (GnT-V) and alpha2,6 sialyltransferase (ST6GalI) to address the important roles of N-glycans in integrin-meditaed cell adhesion and migration. 29212317 5 5 gly heterogeneity 859:871 arg1 Apo CIII Apo CIII PUBTATOR form of Apo CIII 345 Here, we profile the O- and N-linked glycosylation of HDL associated-proteins including the truncated form of Apo CIII and their glycan heterogeneity in a site-specific manner. 29212317 5 22 gly glycosylation 760:772 arg1 HDL associated-proteins HDL associated-proteins OGER HDL associated-proteins Q9UNE0 Here, we profile the O- and N-linked glycosylation of HDL associated-proteins including the truncated form of Apo CIII and their glycan heterogeneity in a site-specific manner. 29212317 5 68 gly CIII 837:840 arg1 their glycan heterogeneity form of Apo CIII their glycan heterogeneity PUBTATOR form of Apo CIII 345 Here, we profile the O- and N-linked glycosylation of HDL associated-proteins including the truncated form of Apo CIII and their glycan heterogeneity in a site-specific manner. 7964612 11 102 gly unglycosylated 1718:1731 arg1 no unglycosylated M protein no unglycosylated M protein OGER M protein P54296 (4) Normally, no unglycosylated M protein was secreted. 10556243 1 33 gly glycoprotein 225:236 arg1 The endothelial cell receptor (EPCR) for protein C (PC)/activated protein C The endothelial cell receptor (EPCR) for protein C (PC)/activated protein C OGER protein C P02810 The endothelial cell receptor (EPCR) for protein C (PC)/activated protein C (APC) is a 221 amino-acid residues long transmembrane glycoprotein with unclear physiological function. 12645620 4 2 gly alpha2-HS-glycoprotein 723:744 arg1 alpha2-HS-glycoprotein alpha2-HS-glycoprotein OGER alpha2-HS-glycoprotein P02765 Oligosaccharides from the major plasma glycoproteins with a pI between 4 and 7 were characterized from the glycoforms of haptoglobin, alpha2-HS-glycoprotein, serotransferrin, alpha1-antitrypsin, and alpha1-antichymotrypsin. 12645620 4 8 gly glycoforms 696:705 arg1 haptoglobin haptoglobin OGER haptoglobin P00738 Oligosaccharides from the major plasma glycoproteins with a pI between 4 and 7 were characterized from the glycoforms of haptoglobin, alpha2-HS-glycoprotein, serotransferrin, alpha1-antitrypsin, and alpha1-antichymotrypsin. 12645620 4 8 gly glycoforms 696:705 arg1 alpha1-antitrypsin alpha1-antitrypsin OGER alpha1-antitrypsin P01009 Oligosaccharides from the major plasma glycoproteins with a pI between 4 and 7 were characterized from the glycoforms of haptoglobin, alpha2-HS-glycoprotein, serotransferrin, alpha1-antitrypsin, and alpha1-antichymotrypsin. 12645620 4 8 gly glycoforms 696:705 arg1 alpha1-antichymotrypsin alpha1-antichymotrypsin OGER alpha1-antichymotrypsin P01011 Oligosaccharides from the major plasma glycoproteins with a pI between 4 and 7 were characterized from the glycoforms of haptoglobin, alpha2-HS-glycoprotein, serotransferrin, alpha1-antitrypsin, and alpha1-antichymotrypsin. 12645620 4 8 gly glycoforms 696:705 arg1 serotransferrin serotransferrin OGER serotransferrin P02787 Oligosaccharides from the major plasma glycoproteins with a pI between 4 and 7 were characterized from the glycoforms of haptoglobin, alpha2-HS-glycoprotein, serotransferrin, alpha1-antitrypsin, and alpha1-antichymotrypsin. 12645620 4 8 gly glycoforms 696:705 arg1 alpha2-HS-glycoprotein alpha2-HS-glycoprotein OGER alpha2-HS-glycoprotein P02765 Oligosaccharides from the major plasma glycoproteins with a pI between 4 and 7 were characterized from the glycoforms of haptoglobin, alpha2-HS-glycoprotein, serotransferrin, alpha1-antitrypsin, and alpha1-antichymotrypsin. 27320199 5 57 gly deglycosylation 722:736 arg1 IgG hexamerization IgG hexamerization Cterm IgG Fc-domain deglycosylation, described to abrogate complement activation, affected IgG hexamerization and C1q binding. 27320199 5 57 gly deglycosylation 722:736 arg1 C1q binding C1q binding PUBTATOR C1q 712 Fc-domain deglycosylation, described to abrogate complement activation, affected IgG hexamerization and C1q binding. 26699903 0 58 gly SCUBE1 59:64 arg1 N-glycan-mediated membrane tethering SCUBE1 N-glycan-mediated membrane tethering PUBTATOR SCUBE1 797832 Electrostatics and N-glycan-mediated membrane tethering of SCUBE1 is critical for promoting bone morphogenetic protein signalling. 2009524 10 18 gly nonglycosylated 1649:1663 arg1 glycosylated and nonglycosylated GCAP glycosylated and nonglycosylated GCAP PUBTATOR GCAP 251 Moreover, the specific enzyme activity of glycosylated and nonglycosylated GCAP remained unchanged, indicating that AP lacking N-linked oligosaccharide side chains was catalytically active. 2009524 10 22 gly glycosylated 1632:1643 arg1 glycosylated and nonglycosylated GCAP glycosylated and nonglycosylated GCAP PUBTATOR GCAP 251 Moreover, the specific enzyme activity of glycosylated and nonglycosylated GCAP remained unchanged, indicating that AP lacking N-linked oligosaccharide side chains was catalytically active. 10920259 2 98 gly deglycosylated 389:402 arg1 human deglycosylated mucin human deglycosylated mucin PUBTATOR mucin 100508689 To investigate the tumor-associated expression, MUC4 cDNA was cloned from a human pancreatic tumor cell line cDNA expression library using a polyclonal antibody raised against human deglycosylated mucin and RT-PCR. 18824109 5 15 gly glycoprotein 942:953 arg1 carp Tf carp Tf Cterm Tf P02787 In contrast to human Tf, we could not detect potential N-glycosylation sites in carp Tf, which does not seem to be a glycoprotein. 2223825 0 70 gly receptor 52:59 arg1 N-linked oligosaccharides transferrin receptor N-linked oligosaccharides PUBTATOR transferrin receptor 22041 N-linked oligosaccharides of the murine transferrin receptor from a plasmacytoma cell line. 22601780 3 6 gly contains 344:351 arg1 Del-1 AND 3 epidermal growth factor (EGF)-like repeats Del-1 3 epidermal growth factor (EGF)-like repeats PUBTATOR Del-1 10085 Del-1 contains 3 epidermal growth factor (EGF)-like repeats and 2 discoidin-like domains. 22344255 1 5 gly N-glycosylation 109:123 arg1 neural cadherin neural cadherin OGER neural cadherin P19022 We present direct evidence that the N-glycosylation state of neural cadherin impacts the intrinsic kinetics of cadherin-mediated intercellular binding. 10364201 8 82 gly N-glycosylated 1188:1201 arg1 N-glycosylated AE1 N-glycosylated AE1 PUBTATOR N-glycosylated AE1 6521 An association of calnexin with truncated versions of N-glycosylated AE1 was detected after release of the nascent chains from ribosomes with puromycin. 28614667 8 46 part_of CTR 1582:1584 arg1 CTR N130 CTR CTR N130 PUBTATOR SpecificSite CTR 799 N130 Characterization of peptide-binding affinities of purified N → Q CTR ECD glycan site mutants combined with PNGase F and Endo H treatment strategies and mass spectrometry to define the glycan species indicated that a single GlcNAc residue at CTR N130 was responsible for the peptide affinity enhancement. 26125800 1 63 gly CMP 134:136 arg1 NeuNAc CMP NeuNAc OGER CMP P21941 Cytidine monophosphate (CMP) N-acetylneuraminic acid (NeuNAc) synthetase, which is encoded by the neuA gene, can catalyze the activation of sialic acid with CMP, and plays an important role in Streptococcus agalactiae infection pathogenesis. 16510764 8 12 gly O-glycosylation 977:991 arg1 IgAN IgAN PUBTATOR IgAN 60498 Defective O-glycosylation of IgA1, probably taking the form of reduced galactosylation, was confirmed in IgAN in this study. 16510764 8 12 gly O-glycosylation 977:991 arg1 IgA1 IgA1 PUBTATOR IgA1 3493 Defective O-glycosylation of IgA1, probably taking the form of reduced galactosylation, was confirmed in IgAN in this study. 24489700 7 3 gly non-glycosylated 1610:1625 arg1 non-glycosylated Grp94 non-glycosylated Grp94 PUBTATOR Grp94 7184 ATP does not affect at all the binding capacity of non-glycosylated Grp94. 24489700 7 67 gly Grp94 1627:1631 arg1 all the binding capacity Grp94 all the binding capacity PUBTATOR Grp94 7184 ATP does not affect at all the binding capacity of non-glycosylated Grp94. 1370171 9 64 gly glycosylated 1538:1549 arg1 the heavily glycosylated leukocyte surface molecule CD43 the heavily glycosylated leukocyte surface molecule CD43 PUBTATOR CD43 6693 CD34 has no significant sequence homology to any known protein but has some structural similarities to the heavily glycosylated leukocyte surface molecule CD43. 11741940 8 32 gly moiety 1571:1576 arg1 Asn Asn Asn SpecificSite Asn(172) Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 11741940 8 32 gly moiety 1571:1576 arg1 172 172 172 SpecificSite Asn(172) Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 11741940 8 87 gly contains 1530:1537 arg1 sFRP-1 AND a relatively large carbohydrate moiety sFRP-1 a relatively large carbohydrate moiety PUBTATOR sFRP-1 6422 Analysis of glycosylation sites showed that sFRP-1 contains a relatively large carbohydrate moiety on Asn(172) (approximately 2.8 kDa), whereas Asn(262), the second potential N-linked glycosylation site, is not modified. 6836913 6 32 gly glycopeptides 749:761 arg1 gp52 gp52 Cterm gp52 Analysis of tryptic glycopeptides from gp52 by reverse-phase high-performance liquid chromatography also suggested the presence of four glycosylation sites. 10971587 10 10 gly deglycosylated 1334:1347 arg1 NPR-ECD NPR-ECD OGER ECD O95905 NPR-ECD deglycosylated with endoglycosidase F2 and endoglycosidase H retained ANP-binding activity and showed an affinity for ANP similar to that of untreated NPR-ECD. 2294110 7 36 gly desialylated 1310:1321 arg1 the desialylated rFib2 protein the desialylated rFib2 protein Cterm rFib2 protein 14119 The presence of O-linked but absence of N-linked glycans was further supported by the observations that peanut agglutinin specifically bound to the desialylated rFib2 protein, whereas neither concanavalin A nor lentil lectin bound to the protein irrespective of prior neuraminidase treatment. 16085713 6 86 part_of Ser-1177 1213:1220 arg1 Akt Akt Ser-1177 PUBTATOR SpecificSite Akt 24185 Ser-1177 Phosphorylation of Akt, a mediator of shear stress-induced eNOS phosphorylation at Ser-1177, was decreased in the diabetic penis at baseline, but it was restored by ES. 2340332 8 39 gly hTeBG 1467:1471 arg1 the biantennary complex oligosaccharides TeBG the biantennary complex oligosaccharides OGER TeBG About 8% of the biantennary complex oligosaccharides on hTeBG and none of those on rbTeBG were fucosylated on the chitobiose core, as determined by chromatography on Lens culinaris lectin (LcH). 2340332 8 43 gly oligosaccharides 1447:1462 arg1 hTeBG TeBG oligosaccharides OGER TeBG About 8% of the biantennary complex oligosaccharides on hTeBG and none of those on rbTeBG were fucosylated on the chitobiose core, as determined by chromatography on Lens culinaris lectin (LcH). 11672902 6 57 gly containing 762:771 arg1 rgp160 protein AND N-linked glycans rgp160 protein N-linked glycans PUBTATOR gp160 protein 2028 Two additional groups were primed with wild type or mutant env and boosted with rgp160 protein, containing the complete set of N-linked glycans. 19195686 5 95 gly glycosylated 746:757 arg1 these CCK receptors these CCK receptors PUBTATOR CCK receptors 25298 The lectins wheat germ agglutinin (WGA) and Ulex europaeus agglutinin (UEA-I) bind to the glycosylated sites of these CCK receptors with the effect inhibiting CCK binding and thus inhibiting the CCK-induced Ca2+ release and alpha-amylase secretion. 1649338 0 19 gly D 64:64 arg1 asparagine-linked oligosaccharides glycoprotein D asparagine-linked oligosaccharides PUBTATOR glycoprotein D 2532 Absence of asparagine-linked oligosaccharides from glycoprotein D of herpes simplex virus type 1 results in a structurally altered but biologically active protein. 1649338 0 67 gly glycoprotein 51:62 arg1 glycoprotein D glycoprotein D PUBTATOR glycoprotein D 2532 Absence of asparagine-linked oligosaccharides from glycoprotein D of herpes simplex virus type 1 results in a structurally altered but biologically active protein. 26873173 6 10 gly Hp 907:908 arg1 glycan Hp glycan Cterm Hp 3240 In this mini review, we provided a brief overview of Hp structure and biological function, discussed its glycosylation alterations in different cancers, and described the existing technologies for analyzing glycosylation site and glycan of Hp. 26873173 6 35 gly glycosylation 874:886 arg1 Hp Hp Cterm Hp 3240 In this mini review, we provided a brief overview of Hp structure and biological function, discussed its glycosylation alterations in different cancers, and described the existing technologies for analyzing glycosylation site and glycan of Hp. 1476702 1 19 gly containing 129:138 arg1 rat transferrin AND a single hybrid glycan rat transferrin a single hybrid glycan PUBTATOR transferrin 24825 Production of rat transferrin containing a single hybrid glycan was induced by treating rats with swainsonine, an inhibitor of alpha-mannosidase II. 7615513 3 49 gly polysialylated 541:554 arg1 polysialylated NCAM polysialylated NCAM PUBTATOR NCAM 17967 Chicken NCAM cDNAs containing amino acid mutations, domain deletions, and domain substitutions were expressed in the F11 rat/mouse hybrid cell line, which can produce polysialylated NCAM. 18952826 0 33 gly glycoforms 125:134 arg1 IgG glycoforms IgG glycoforms Cterm IgG The N-linked oligosaccharide at Fc gamma RIIIa Asn-45: an inhibitory element for high Fc gamma RIIIa binding affinity to IgG glycoforms lacking core fucosylation. 18952826 0 43 gly RIIIa 41:45 arg1 The N-linked oligosaccharide Fc gamma RIIIa The N-linked oligosaccharide PUBTATOR Fc gamma RIIIa 2214 The N-linked oligosaccharide at Fc gamma RIIIa Asn-45: an inhibitory element for high Fc gamma RIIIa binding affinity to IgG glycoforms lacking core fucosylation. 9820842 7 16 gly glycoprotein 1331:1342 arg1 US5 US5 PUBTATOR US5 2703406 These findings establish that US5 does encode a glycoprotein and confirm the appropriateness of naming the US5 gene product gJ. 22288421 2 53 gly glycoproteins 273:285 arg1 CD43 CD43 PUBTATOR CD43 6693 CD43 and CD45 are the two most abundant glycoproteins on the T cell surface and are decorated with O- and N-glycans. 22288421 2 53 gly glycoproteins 273:285 arg1 CD45 CD45 PUBTATOR CD45 5788 CD43 and CD45 are the two most abundant glycoproteins on the T cell surface and are decorated with O- and N-glycans. 26979432 3 45 gly VN 447:448 arg1 sialylation VN sialylation PUBTATOR VN 22370 In this study, we analyzed the mechanism how sialylation of VN regulates the properties of mouse primary cultured dermal fibroblasts (MDF) and a dermal fibroblast cell line, Swiss 3T3 cells. 26979432 3 96 gly sialylation 432:442 arg1 VN VN PUBTATOR VN 22370 In this study, we analyzed the mechanism how sialylation of VN regulates the properties of mouse primary cultured dermal fibroblasts (MDF) and a dermal fibroblast cell line, Swiss 3T3 cells. 10226612 2 27 gly glycoprotein 362:373 arg1 the human cytomegalovirus (HCMV) glycoprotein H the human cytomegalovirus (HCMV) glycoprotein H Cterm the human cytomegalovirus (HCMV) glycoprotein H (gH Since the in vivo co-expression of the human cytomegalovirus (HCMV) glycoprotein H (gH, gpUL75) with glycoprotein L (gL, gpUL115) may have relevance to CMV vaccine studies, these experiments were undertaken to test whether the GPCMV encodes a gL homolog. 26657071 0 12 gly Aglycosylated 61:73 arg1 Aglycosylated Human Carboxylesterase 1 Aglycosylated Human Carboxylesterase 1 OGER Carboxylesterase 1 P23141 Comparison of the Structure and Activity of Glycosylated and Aglycosylated Human Carboxylesterase 1. 10353820 5 11 gly fucosylated 795:805 arg1 fucosylated FVII EGF-1 fucosylated FVII EGF-1 OGER FVII EGF-1 P08709 The overall structure of fucosylated FVII EGF-1 is very similar to the nonfucosylated form even for the residues near the fucosylation site. 19060393 0 17 gly glycoprotein 229:240 arg1 mammalian deoxyribonuclease I. Deoxyribonuclease I mammalian deoxyribonuclease I. Deoxyribonuclease I PUBTATOR I (DNase I 1773 Two N-linked glycosylation sites (Asn18 and Asn106) are both required for full enzymatic activity, thermal stability, and resistance to proteolysis in mammalian deoxyribonuclease I. Deoxyribonuclease I (DNase I) is known to be a glycoprotein, and two potential N-linked glycosylation sites (N18 and N106) are known for mammalian enzymes. 7479385 7 12 gly PSA 1222:1224 arg1 The experimentally determined carbohydrate content PSA The experimentally determined carbohydrate content PUBTATOR PSA 354 The experimentally determined carbohydrate content of PSA confirms that only one N-glycosylation site is occupied in the protein. 9239700 6 34 gly contains 1341:1348 arg1 rhFSH AND fewer complex forms rhFSH fewer complex forms OGER rhFSH In summary, rhFSH contains fewer complex forms and an increased proportion of simple carbohydrate structures in comparison with Metrodin, Metrodin-HP and IS 70/45. 10397151 10 55 gly RIIa 1294:1297 arg1 core mannose oligosaccharide side chains Fc gamma RIIa core mannose oligosaccharide side chains PUBTATOR Fc gamma RIIa 2212 Electrospray ionisation mass spectrometry (ESMS) indicate that the N-glycans of baculovirus derived Fc gamma RIIa are core mannose oligosaccharide side chains. 10397151 10 55 gly RIIa 1294:1297 arg1 the N-glycans Fc gamma RIIa the N-glycans PUBTATOR Fc gamma RIIa 2212 Electrospray ionisation mass spectrometry (ESMS) indicate that the N-glycans of baculovirus derived Fc gamma RIIa are core mannose oligosaccharide side chains. 19690161 6 23 gly glycosylation 736:748 arg1 functional vIL-6 functional vIL-6 OGER vIL P09327 N-Linked glycosylation at the Asn-89 site was required for intracellular production of functional vIL-6, but endoglycosidase-mediated removal of N-linked glycans from secreted vIL-6 did not impair protein function. 29642453 3 59 gly glycosylation 439:451 arg1 NA NA Cterm NA 4758 We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century. 29642453 3 59 gly glycosylation 439:451 arg1 over 10,000 HA over 10,000 HA Cterm 10,000 HA We monitored the potential N-linked glycosylation (NLG) sites of over 10,000 HA and NA of H1N1 subtype isolated from human, avian, and swine species over the past century. 19501045 1 25 gly glycoprotein 120:131 arg1 gp130 gp130 PUBTATOR gp130 16195 gp130 is a ubiquitously expressed glycoprotein and signal transducer of interleukin 6 family of cytokines. 26585416 0 90 gly N-glycosylation 10:24 arg1 the peptide transporter PEPT1 the peptide transporter PEPT1 PUBTATOR PEPT1 56643 Effect of N-glycosylation on the transport activity of the peptide transporter PEPT1. 20622017 4 62 gly released 711:718 arg2 isolated GGT AND glycans isolated GGT glycans OGER GGT Recent advances in mass spectrometry enabled us to identify the microheterogeneity and relative abundance of glycans on specific glycopeptides and revealed a broader spectrum of glycans than was observed among glycans enzymatically released from isolated GGT. 25016576 0 63 gly epitope 70:76 arg1 the HIV-1 envelope protein envelope protein epitope PUBTATOR envelope protein 100616444 Characterization of a monoclonal antibody to a novel glycan-dependent epitope in the V1/V2 domain of the HIV-1 envelope protein, gp120. 9244386 0 35 gly Glycosylation 0:12 arg1 bile-salt-stimulated lipase bile-salt-stimulated lipase PUBTATOR bile-salt-stimulated lipase 1056 Glycosylation of bile-salt-stimulated lipase from human milk: comparison of native and recombinant forms. 16407218 3 74 gly glycosylated 511:522 arg1 TEP TEP Cterm TEP We isolated the serum protease inhibitor alpha(2) macroglobulin (alpha2M), a heavily glycosylated thiol ester protein (TEP) composed of four identical 180-kDa subunits, each of which has eight N-linked glycosylation sites. 16407218 3 74 gly glycosylated 511:522 arg1 the serum protease inhibitor alpha(2) macroglobulin the serum protease inhibitor alpha(2) macroglobulin PUBTATOR alpha(2) macroglobulin 2 We isolated the serum protease inhibitor alpha(2) macroglobulin (alpha2M), a heavily glycosylated thiol ester protein (TEP) composed of four identical 180-kDa subunits, each of which has eight N-linked glycosylation sites. 23527852 0 86 gly glycoforms 42:51 arg1 new apolipoprotein-CIII glycoforms new apolipoprotein-CIII glycoforms PUBTATOR apolipoprotein-CIII 345 Identification of new apolipoprotein-CIII glycoforms with ultrahigh resolution MALDI-FTICR mass spectrometry of human sera. 10620506 6 31 gly multi-glycosylated 785:802 arg1 MGC-24v MGC-24v PUBTATOR MGC-24v 8763 Endolyn is closely related to two human mucin-like proteins, multi-glycosylated core protein (MGC)-24 and CD164 (MGC-24v), expressed in gastric carcinoma cells and bone marrow stromal and haematopoietic precursor cells respectively. 10620506 6 31 gly multi-glycosylated 785:802 arg1 MGC multi-glycosylated core protein (MGC)-24 PUBTATOR multi-glycosylated core protein (MGC)-24 8763 Endolyn is closely related to two human mucin-like proteins, multi-glycosylated core protein (MGC)-24 and CD164 (MGC-24v), expressed in gastric carcinoma cells and bone marrow stromal and haematopoietic precursor cells respectively. 17983356 2 4 part_of sOb-R 269:273 arg1 residues 22-839 of the extracellular domain sOb-R residues 22-839 of the extracellular domain Cterm SpecificSite sOb-R 3953 residues 22-839 We found that a mammalian cell-expressed sOb-R (soluble Ob-R) fragment (residues 22-839 of the extracellular domain) bound leptin with a dissociation constant of 1.8 nM. 17983356 2 55 part_of Ob-R 284:287 arg1 residues 22-839 of the extracellular domain Ob-R residues 22-839 of the extracellular domain PUBTATOR SpecificSite Ob-R 3953 residues 22-839 We found that a mammalian cell-expressed sOb-R (soluble Ob-R) fragment (residues 22-839 of the extracellular domain) bound leptin with a dissociation constant of 1.8 nM. 19114500 2 65 gly glycoprotein 769:780 arg1 VSG VSG Cterm VSG These analyses highlight peculiarities of the Trypanosoma brucei UDP-glucose:glycoprotein glucosyltransferase, including an unusually wide substrate specificity, ranging from Man(5)GlcNAc(2) to Man(9)GlcNAc(2) glycans, and an unusually high efficiency in vivo, quantitatively glucosylating the Asn263 N-glycan of variant surface glycoprotein (VSG) 221 and 75% of all non-VSG N glycosylation sites. 2543790 1 15 gly glycoprotein 308:319 arg1 glycoprotein C glycoprotein C Cterm 1 glycoprotein C Three monoclonal antibodies (MAbs) have been raised against cell membrane-derived herpes simplex virus type 1 glycoprotein C (gC-1). 26972002 0 4 gly Glycoprotein 90:101 arg1 Individual Glycan Sites Envelope Glycoprotein Individual Glycan Sites Cterm Envelope Glycoprotein 155971 Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein. 29587225 1 7 gly glycoprotein 186:197 arg1 Recombinant human erythropoietin Recombinant human erythropoietin PUBTATOR erythropoietin 2056 Recombinant human erythropoietin (EPO) is a therapeutic glycoprotein widely used for treating anemia. 26208754 8 50 gly un-glycosylated 1153:1167 arg1 The un-glycosylated mutant bcIFNa-N38Q The un-glycosylated mutant bcIFNa-N38Q Cterm bcIFNa The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. 7492680 4 67 gly glycoprotein 616:627 arg1 oviductin oviductin OGER oviductin Q12889 Based on comparisons with the N-terminal amino acid sequences of purified-BOGP and of hamster oviduct-specific glycoprotein (oviductin), it was inferred that the derived amino acid sequence contained a signal peptide region of 21 amino acids and a mature MOGP (core protein) region of 700 amino acids (76,515 daltons). 7492680 4 67 gly glycoprotein 616:627 arg1 hamster oviduct-specific glycoprotein hamster oviduct-specific glycoprotein PUBTATOR oviduct-specific glycoprotein 5016 Based on comparisons with the N-terminal amino acid sequences of purified-BOGP and of hamster oviduct-specific glycoprotein (oviductin), it was inferred that the derived amino acid sequence contained a signal peptide region of 21 amino acids and a mature MOGP (core protein) region of 700 amino acids (76,515 daltons). 17178884 3 70 gly glycosylated 498:509 arg1 MDA-7/IL-24 MDA-7/IL-24 PUBTATOR Because MDA-7 11009 Because MDA-7/IL-24 is a glycosylated protein, we investigated the role of glycosylation in mediating the specific biological and "bystander" antitumor activities of this cytokine. 26869352 11 29 gly fucosylated 1824:1834 arg1 fucosylated haptoglobin fucosylated haptoglobin PUBTATOR haptoglobin 3240 These data suggest that the original tissue/cell producing fucosylated haptoglobin is different in each cancer type and linkage of fucosylation might be a clue of primary lesion, thereby enabling a differential diagnosis between gastroenterological cancers and non-gastroenterological cancers. 15084583 3 47 part_of PrPSc 766:770 arg1 residues 23, 24, and 27 Sc residues 23, 24, and 27 Cterm SpecificSite Sc residues 23, 24, and 27 We report that in animal and human TSEs, one or more lysines at residues 23, 24, and 27 of PrPSc are covalently modified with advanced glycosylation end products (AGEs), which may be carboxymethyl-lysine (CML), one of the structural varieties of AGEs. 28810662 6 20 gly O-glycans 773:781 arg1 PDI PDI O-glycans PUBTATOR PDI 5034 O-glycans on PDI are required for galectin-9 binding, and PDI recognition appears to be specific for galectin-9, as galectin-1 and galectin-3 do not bind PDI. 14715137 0 25 gly N-glycosylation 0:14 arg1 synaptotagmin 1 synaptotagmin 1 PUBTATOR synaptotagmin 1 6857 N-glycosylation is essential for vesicular targeting of synaptotagmin 1. 27258397 1 0 gly glycan 196:201 arg1 gp120 gp120 glycan PUBTATOR gp120 155971 The glycan shield of the HIV-1 envelope glycoprotein complex (Env), in particular the glycan at position 332 in gp120, is frequently targeted by broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals. 28955860 6 9 gly deglycosylated 736:749 arg1 Both mature and deglycosylated GASP-2 proteins Both mature and deglycosylated GASP-2 proteins PUBTATOR GASP-2 proteins 215001 RESULTS: Both mature and deglycosylated GASP-2 proteins increase C2C12 proliferation and differentiation by inhibiting the myostatin pathway. 12438572 3 67 part_of GP 596:597 arg1 Cys-53 GP(1 Cys-53 OGER SpecificSite GP(1 O00178 Cys-53 Cys-53 was the only GP(1) ( approximately 130 kDa) cysteine residue whose replacement resulted in the efficient secretion of GP(1), and it is therefore proposed that it participates in the formation of the only disulfide bond linking GP(1) to GP(2) ( approximately 24 kDa). 19409386 1 33 gly glycosylated 243:254 arg1 the proton-coupled amino acid transporter 1 the proton-coupled amino acid transporter 1 OGER proton-coupled amino acid transporter 1 Q7Z2H8 In the present study we show in the Xenopus laevis expression system that the proton-coupled amino acid transporter 1 (PAT1, SLC36A1) is glycosylated at asparagine residues N174, N183 and N470. 9422088 1 0 gly apoprotein 298:307 arg1 the tandem repeat region MUC1 apoprotein the tandem repeat region PUBTATOR MUC1 apoprotein 4582 The specificities of the 56 MAbs submitted to the ISOBM TD-4 Workshop were characterized by ELISA assays against MUC1-positive (ZR75-1) and MUC1-negative cell lines (LS174T), binding to a 20-mer peptide corresponding to the tandem repeat region of MUC1 apoprotein, and Pepscan analysis of overlapping 9-mer peptides corresponding to the tandem repeat region of MUC1. 9422088 1 9 gly MUC1 406:409 arg1 the tandem repeat region MUC1 the tandem repeat region PUBTATOR MUC1 4582 The specificities of the 56 MAbs submitted to the ISOBM TD-4 Workshop were characterized by ELISA assays against MUC1-positive (ZR75-1) and MUC1-negative cell lines (LS174T), binding to a 20-mer peptide corresponding to the tandem repeat region of MUC1 apoprotein, and Pepscan analysis of overlapping 9-mer peptides corresponding to the tandem repeat region of MUC1. 1584800 4 73 gly 3:2:1 512:516 arg1 mannose/glucosamine/fucose 2:1 mannose/glucosamine/fucose OGER 2:1 P35326 Two N-linked glycosylation sites contain equal ratios of mannose/glucosamine/fucose of 3:2:1. 29793953 12 25 gly glycosylated 2294:2305 arg1 NTCP NTCP PUBTATOR NTCP 6554 NTCP introduced to HepG2 cells was glycosylated at two N-linked glycosylation sites, but mutating either or both sites failed to prevent infection by cell culture-derived HBV or to confer susceptibility to serum-derived HBV. 12901863 0 26 gly N-glycosylation 29:43 arg1 human ABCC6 human ABCC6 PUBTATOR ABCC6 368 Subcellular localization and N-glycosylation of human ABCC6, expressed in MDCKII cells. 20943674 8 25 gly glycosylated 1364:1375 arg1 rhLF rhLF OGER rhLF P02788 Monosaccharide composition analysis of rhLF revealed small amounts of N-glycolylneuraminic acid, which were not detected by MS. hLF and rhLF appear to be glycosylated at the same two sites: Asn138 and Asn479. 20943674 8 25 gly glycosylated 1364:1375 arg1 hLF hLF PUBTATOR hLF 3131 Monosaccharide composition analysis of rhLF revealed small amounts of N-glycolylneuraminic acid, which were not detected by MS. hLF and rhLF appear to be glycosylated at the same two sites: Asn138 and Asn479. 1367474 3 4 gly glycosylation 480:492 arg1 tPA tPA PUBTATOR tPA 100128998 Employing one of these strains to study the effect of glycosylation on secretion, we show that glycosylation in the catalytic domain of tPA plays an important role in folding and/or secretion of the molecule. 24342833 3 35 gly β-catenin 489:497 arg1 the putative O-GlcNAc sites -catenin the putative O-GlcNAc sites PUBTATOR -catenin 1499 OBJECTIVE: The objectives of this investigation were to identify the putative O-GlcNAc sites of β-catenin and the relevance of identified sites in the regulation of β-catenin's localization and transcriptional activity. 3030729 11 6 gly apolipoproteins 1413:1427 arg1 the tandem repeats apolipoproteins the tandem repeats PUBTATOR apolipoproteins 348 ApoB lacks the tandem repeats which are characteristic of other apolipoproteins. 9433921 8 39 gly glycosylation 1177:1189 arg1 OTR OTR PUBTATOR OTR 5021 This suggests that the full glycosylation of OTR observed in vivo is not essential for its activity. 12061794 8 25 gly possess 1123:1129 arg1 Fugu PrP-like AND tandem repeats Fugu PrP-like a region tandem repeats PUBTATOR Site PrP 5621 region However, Fugu PrP-like does not possess tandem repeats or a region with two glycosylation sites and a disulphide bridge. 25990233 4 34 gly glycosylation 870:882 arg1 HA HA Cterm HA Drift variants contained HA substitutions and alterations in the potential N-linked glycosylation sites of HA. 9407058 5 102 gly N-glycosylated 732:745 arg1 PTX3 PTX3 PUBTATOR PTX3 100754664 Lectin binding and treatment with N-glycosidase F showed that PTX3 is N-glycosylated, sugars accounting for 5 kDa of the monomer mass (45 kDa). 18381078 2 19 gly contain 340:346 arg1 ADAM10 AND high-mannose and complex-type glycans ADAM10 high-mannose and complex-type glycans PUBTATOR ADAM10 102 In this work, ADAM10 was found to contain high-mannose and complex-type glycans. 7592666 3 16 gly glycosylation 559:571 arg1 D. cathepsin D PUBTATOR cathepsin D 1509 The deduced amino acid sequence has four potential glycosylation sites, two of which are in identical positions to the two glycosylation sites of human kidney lysosomal cathepsin D. Furthermore, all four disulfide bonds found in mammalian cathepsin D sequences are present in Sjpasp, although the beta-hairpin (loop 3), which is cleaved during maturation of vertebrate cathepsin Ds to yield light and heavy chain subunits, is absent from Sjpasp. 9721187 9 5 gly glycosylation 1297:1309 arg1 p62 p62 PUBTATOR p62 18226 The glycosylation of p62 in these cells appears to involve only single O-linked GlcNAc moieties; no significant sialation was detected. 9171889 10 71 gly carbohydrate 1311:1322 arg1 C8 beta C8 beta carbohydrate PUBTATOR C8 beta 732 Furthermore, N-linked carbohydrate on C8 beta is not necessary for association with C8 alpha-gamma or for C8 activity. 7871762 1 11 gly glycoprotein 233:244 arg1 its glycoprotein B its glycoprotein B PUBTATOR glycoprotein B 920212 In order to estimate the phylogenetic relationship of BHV-4 among the herpesviruses, we have cloned and sequenced its glycoprotein B (gB). 3198605 0 25 gly glycoproteins 51:63 arg1 h-lamp-2 h-lamp-2 PUBTATOR lamp-2 3920 Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. 3198605 0 25 gly glycoproteins 51:63 arg1 h-lamp-1 h-lamp-1 PUBTATOR lamp-1 3916 Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. 25081999 5 50 gly hSCP1 1014:1018 arg1 the O-GlcNAc modification site hSCP1 the O-GlcNAc modification site PUBTATOR hSCP1 58190 To gain insight into the PTM of hSCP1, we used the Western blot, immunoprecipitation, succinylayed wheat germ agglutininprecipitation, liquid chromatography-mass spectrometry analyses, and site-directed mutagenesis and identified the Ser41 residue of hSCP1 as the O-GlcNAc modification site. 10383441 0 0 gly I 83:83 arg1 mannose phosphorylation DNase I mannose phosphorylation PUBTATOR DNase I 13419 Identification of amino acids that modulate mannose phosphorylation of mouse DNase I, a secretory glycoprotein. 10383441 0 36 gly glycoprotein 98:109 arg1 mouse DNase I mouse DNase I PUBTATOR DNase I 13419 Identification of amino acids that modulate mannose phosphorylation of mouse DNase I, a secretory glycoprotein. 15671042 8 56 gly N-glycosylated 1427:1440 arg1 the hFKBP65 protein the hFKBP65 protein PUBTATOR hFKBP65 protein 60681 Cellular localization and glycosylation studies revealed the hFKBP65 protein to be localized in the endoplasmic reticulum, and to be N-glycosylated. 24213971 1 37 gly glycoproteins 240:252 arg1 GABAA receptors GABAA receptors PUBTATOR GABAA receptors 14405 GABAA receptors, the major mediators of fast inhibitory neuronal transmission, are heteropentameric glycoproteins assembled from a panel of subunits, usually including α and β subunits with or without a γ2 subunit. 15456847 0 50 gly GPI 19:21 arg1 N-glycans GPI N-glycans OGER GPI P06744 N-glycans, not the GPI anchor, mediate the apical targeting of a naturally glycosylated, GPI-anchored protein in polarised epithelial cells. 2119332 0 30 gly glycosylation 60:72 arg1 mouse thyrotropin mouse thyrotropin OGER thyrotropin Susceptibility to endoglycosidase F and H at the individual glycosylation sites of mouse thyrotropin and free alpha-subunits. 7541225 1 12 gly glycoprotein 75:86 arg1 Hemoglobin Hemoglobin OGER Hemoglobin Hemoglobin (Hb) is not a glycoprotein but can easily be glycosylated by a non-enzymatic mechanism. 7541225 1 47 gly glycosylated 106:117 arg1 Hb Hb Cterm Hb Hemoglobin (Hb) is not a glycoprotein but can easily be glycosylated by a non-enzymatic mechanism. 7541225 1 47 gly glycosylated 106:117 arg1 Hemoglobin Hemoglobin OGER Hemoglobin Hemoglobin (Hb) is not a glycoprotein but can easily be glycosylated by a non-enzymatic mechanism. 6400107 7 29 gly glycosylated 925:936 arg1 submaxillary renin submaxillary renin OGER renin P08424 However, glycosylation would occur in the case of renal renin, whereas submaxillary renin is not glycosylated. 1680860 9 22 gly P-glycoprotein 1524:1537 arg1 in vitro synthesized P-glycoprotein in vitro synthesized P-glycoprotein PUBTATOR P-glycoprotein 67078 These findings suggest a new topological model for in vitro synthesized P-glycoprotein which may be relevant to its in vivo topology. 23527023 6 40 gly diglycosylated 1447:1460 arg1 the diglycosylated PrP the diglycosylated PrP PUBTATOR PrP 19122 Furthermore, PrP(V180I) with a typical glycoform profile from cultured cells generates detectable PrP(res) that also contains the diglycosylated PrP in addition to mono- and unglycosylated forms upon PK-treatment. 29881382 6 66 gly present 1197:1203 arg1 the BG505 Env AND the N-glycan "hole" the BG505 Env the N-glycan "hole" Cterm BG505 Env 155971 These antibodies targeted the N-glycan "hole" naturally present on the BG505 Env proximal to residues at positions 230, 241, and 289. 10451222 5 60 gly contained 804:812 arg1 This hCG isoform AND 100% tetrasaccharide-core O-linked carbohydrate moieties This hCG isoform 100% tetrasaccharide-core O-linked carbohydrate moieties PUBTATOR hCG isoform 93659 This hCG isoform was not only 100% nicked, but also contained 100% tetrasaccharide-core O-linked carbohydrate moieties in its beta COOH-terminal region. 1993697 14 62 gly glycoprotein 1437:1448 arg1 CD63 CD63 PUBTATOR CD63 12512 By immunoelectron microscopy, co-localization with the lysosomal glycoproteins lamp-1 and -2 identified CD63 as a novel lysosomal membrane glycoprotein. 8870657 1 3 gly glycoprotein 164:175 arg1 Human lactoferrin Human lactoferrin OGER Human lactoferrin P02788 Human lactoferrin (hLF) is a glycoprotein involved in the host defence against infection and excessive inflammation. 29982679 6 56 gly site 1538:1541 arg1 FH FH site Cterm FH 3075 We find that FH contains mostly α2-6-linked sialic acid, making an intramolecular interaction with its α2-3-sialic acid specific binding site and an associated self-lock mechanism unlikely, substantiate that there is only a single sialic acid binding site in FH and none in FHL-1, and demonstrate direct binding of FH to the nonhuman sialic acid Neu5Gc, supporting the use of FH transgenic mouse models for studies of complement-related diseases. 29982679 6 90 gly contains 1303:1310 arg1 FH AND α2-6-linked sialic acid FH α2-6-linked sialic acid Cterm FH 3075 We find that FH contains mostly α2-6-linked sialic acid, making an intramolecular interaction with its α2-3-sialic acid specific binding site and an associated self-lock mechanism unlikely, substantiate that there is only a single sialic acid binding site in FH and none in FHL-1, and demonstrate direct binding of FH to the nonhuman sialic acid Neu5Gc, supporting the use of FH transgenic mouse models for studies of complement-related diseases. 23714211 0 118 gly N-glycosylation 0:14 arg1 ICAM-2 ICAM-2 PUBTATOR ICAM-2 3384 N-glycosylation of ICAM-2 is required for ICAM-2-mediated complete suppression of metastatic potential of SK-N-AS neuroblastoma cells. 23523791 0 46 gly Mef2D 51:55 arg1 decreased O-GlcNAc glycosylation Mef2D decreased O-GlcNAc glycosylation PUBTATOR Mef2D 17261 Requirement of decreased O-GlcNAc glycosylation of Mef2D for its recruitment to the myogenin promoter. 23523791 0 51 gly glycosylation 34:46 arg1 Mef2D Mef2D PUBTATOR Mef2D 17261 Requirement of decreased O-GlcNAc glycosylation of Mef2D for its recruitment to the myogenin promoter. 25643797 5 8 part_of residues 964:971 arg1 HA1 HA1 residues PUBTATOR SpecificSite HA1 23526 residues 295-297 Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200-202, and had an additional one at residues 295-297 in HA1. 25253346 2 132 gly possesses 252:260 arg1 Env AND conserved antigenic determinants Env the gp120 primary receptor CD4 binding site conserved antigenic determinants PUBTATOR Site Env 100616444 site Env possesses conserved antigenic determinants, such as the gp120 primary receptor CD4 binding site (CD4bs), a known neutralization target. 26231935 2 56 gly N-glycosylation 335:349 arg1 AChE AChE PUBTATOR AChE 43 Several lines of evidence demonstrated that N-glycosylation of AChE affected the enzymatic activity, as well as its biosynthesis. 9587405 1 73 gly has 132:134 arg1 Human blood coagulation factor VII AND unique carbohydrate moieties Human blood coagulation factor VII unique carbohydrate moieties PUBTATOR coagulation factor VII 2155 Human blood coagulation factor VII has unique carbohydrate moieties O-glycosidically linked to serine 52 and serine 60 residues in its first epidermal growth factor-like domain. 16263180 1 26 gly glycoprotein 100:111 arg1 Human P-selectin glycoprotein ligand-1 Human P-selectin glycoprotein ligand-1 PUBTATOR P-selectin glycoprotein ligand-1 617434 Human P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric membrane mucin expressed on leukocytes that binds selectins. 25289757 1 25 gly glycosylated 209:220 arg1 GPCRs GPCRs Cterm GPCRs Numerous G protein-coupled receptors (GPCRs) are glycosylated at extracellular regions. 11361003 1 76 gly O-glycosylated 135:148 arg1 The transferrin receptor The transferrin receptor PUBTATOR transferrin receptor 7037 The transferrin receptor (TfR) is a N- and O-glycosylated transmembrane protein mediating the cellular iron uptake by binding and internalization of diferric transferrin. 9294593 4 21 gly unglycosylated 791:804 arg1 unglycosylated rBPI unglycosylated rBPI Cterm rBPI P17213 Therefore, 93 BPI-ELISA-positive sera and controls were compared in different ELISAs using nBPI, rBPI, unglycosylated rBPI and a 21-kDa amino-terminal fragment of rBPI. 12435749 1 108 gly glycoprotein 216:227 arg1 The rat ileal sodium-dependent bile acid transporter The rat ileal sodium-dependent bile acid transporter OGER ileal sodium-dependent bile acid transporter Q62633 The rat ileal sodium-dependent bile acid transporter (Asbt) is a polytopic membrane glycoprotein, which is specifically expressed on the apical domain of the ileal brush-border membrane. 6327272 2 56 gly glycoproteins 324:336 arg1 E1 E1 Cterm E1 The nucleocapsid protein N is synthesized on free polysomes, whereas the envelope glycoproteins E1 and E2 are translated on the rough endoplasmic reticulum (RER). 25534360 6 22 gly attached 1029:1036 arg1 VEGFR1 AND the negatively charged O-glycans VEGFR1 the negatively charged O-glycans PUBTATOR VEGFR1 2321 Most importantly, the negatively charged O-glycans attached to the third Ig-like domain of VEGFR1 counterbalanced the originally positively charged VEGFR1 backbone, minimizing nonspecific binding of VEGF-Grab to the extracellular matrix, and resulting in greatly improved pharmacokinetic profile. 15807535 9 4 gly glycosylated 1709:1720 arg1 glycosylated ABCG2 glycosylated ABCG2 PUBTATOR ABCG2 9429 Furthermore, in crude membrane preparations, neither the basal nor the prazosin-stimulated ( approximately 2-fold) ATPase activities of ABCG2 (N596Q) were affected compared to glycosylated ABCG2. 17711303 5 65 gly contains 876:883 arg1 the hKOR AND O-linked glycan the hKOR O-linked glycan PUBTATOR hKOR 4986 FLAG-hKOR was reduced to lower Mr bands by neuraminidase and O-glycosidase, indicating that the hKOR contains O-linked glycan. 19671700 5 64 gly containing 908:917 arg1 TSR1 AND a Glc-Fuc disaccharide TSR1 a Glc-Fuc disaccharide PUBTATOR TSR1 55720 Analysis of tryptic fragments of recombinant human punctin-1 by mass spectrometry identified a peptide derived from TSR1 containing the (36)WDAWGPWSECSRTC(49) sequence of interest modified with two mannose residues and a Glc-Fuc disaccharide (O-fucosylation). 19671700 5 64 gly containing 908:917 arg1 TSR1 AND O-fucosylation TSR1 O-fucosylation PUBTATOR TSR1 55720 Analysis of tryptic fragments of recombinant human punctin-1 by mass spectrometry identified a peptide derived from TSR1 containing the (36)WDAWGPWSECSRTC(49) sequence of interest modified with two mannose residues and a Glc-Fuc disaccharide (O-fucosylation). 8505339 0 47 gly glycosylation 12:24 arg1 active human beta-glucuronidase active human beta-glucuronidase PUBTATOR beta-glucuronidase 2990 The role of glycosylation and phosphorylation in the expression of active human beta-glucuronidase. 1379443 9 34 gly linked 1030:1035 arg2 PI AND an oligosaccharide PI an oligosaccharide Cterm (PI) The protein part of the molecule is covalently linked to an oligosaccharide which, in turn, is glycosydically linked to phosphatidylinositol (PI). 14628467 5 20 part_of His-57 1013:1018 arg1 PepT1 PepT1 His-57 PUBTATOR SpecificSite PepT1 6564 His-57 These transporters have some significant molecular characteristics: (1) Contain 12 membrane-spanning domains and a large extracellular loop between transmembrane domains 9 and 10, and all of the transmembrane domains are highly conserved, the extracellular loops are much less so; (2) The encoded proteins have a number of potential N-glycosylation as well as protein kinase recognition sites, which may be involved in the regulation of peptide transport; (3) His-57 in PepT1 and His-87 in PepT2 are the most critical histidyl residues that are necessary for the uptake function and probably represent some critical binding sites; (4) The proteins range in size from 707 to 729 amino acid residues in various species, with higher homology between species for a given transporter (approximately 80%) and less homology between transporters for a given species (approximately 50%). 14628467 5 20 part_of His-57 1013:1018 arg1 PepT2 PepT2 His-57 PUBTATOR SpecificSite PepT2 6565 His-57 These transporters have some significant molecular characteristics: (1) Contain 12 membrane-spanning domains and a large extracellular loop between transmembrane domains 9 and 10, and all of the transmembrane domains are highly conserved, the extracellular loops are much less so; (2) The encoded proteins have a number of potential N-glycosylation as well as protein kinase recognition sites, which may be involved in the regulation of peptide transport; (3) His-57 in PepT1 and His-87 in PepT2 are the most critical histidyl residues that are necessary for the uptake function and probably represent some critical binding sites; (4) The proteins range in size from 707 to 729 amino acid residues in various species, with higher homology between species for a given transporter (approximately 80%) and less homology between transporters for a given species (approximately 50%). 8486716 7 66 gly found 1196:1200 arg2 normal Band 3 AND the polylactosaminyl oligosaccharide normal Band 3 the polylactosaminyl oligosaccharide Cterm Band 3 Enzymatic deglycosylation and tomato lectin binding showed that SAO Band 3 lacked the polylactosaminyl oligosaccharide found on normal Band 3. 15373830 5 63 gly modification 706:717 arg1 HIC1 proteins AND O-GlcNAc modification HIC1 proteins O-GlcNAc modification OGER HIC1 proteins Q14526 Using C-terminal deletion mutants, we have shown that O-GlcNAc modification of HIC1 proteins occurred preferentially in the DNA-binding domain. 15373830 5 79 gly proteins 727:734 arg1 O-GlcNAc modification HIC1 proteins O-GlcNAc modification OGER HIC1 proteins Q14526 Using C-terminal deletion mutants, we have shown that O-GlcNAc modification of HIC1 proteins occurred preferentially in the DNA-binding domain. 28956227 5 10 gly NTPDase3/CD39L3 1088:1102 arg1 four different glycan modifications NTPDase3 four different glycan modifications PUBTATOR NTPDase3 956 To understand the mechanism of the ecto-NTPDase activity and substrate specificity, potentially impacted by N-glycans, we have generated soluble enzymatic domains of NTPDase3/CD39L3 in human embryotic kidney cells with four different glycan modifications. 23001782 4 28 gly glycans 814:820 arg1 intact hSHBG hSHBG glycans PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 91 gly hSHBG 832:836 arg1 detailed glycan structures hSHBG detailed glycan structures PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 91 gly hSHBG 832:836 arg1 glycans hSHBG glycans PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 91 gly hSHBG 832:836 arg1 monosaccharide compositions hSHBG monosaccharide compositions PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 117 gly structures 766:775 arg1 intact hSHBG hSHBG structures PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 23001782 4 124 gly compositions 736:747 arg1 intact hSHBG hSHBG compositions PUBTATOR hSHBG 6462 MS-driven glycoproteomics and glycomics combined with exoglycosidase treatment were used in a bottom-up and top-down manner to determine glycosylation sites, site-specific occupancies and monosaccharide compositions, detailed glycan structures, and the higher level arrangement of glycans on intact hSHBG. 7918455 14 2 gly apo 2176:2178 arg1 the carbohydrate moiety apo(a) the carbohydrate moiety PUBTATOR apo(a) 4018 Changes in the carbohydrate moiety of apo(a), however, do not affect complex formation. 24799124 7 17 gly ceruloplasmin 1559:1571 arg1 core-fucosylation ceruloplasmin core-fucosylation PUBTATOR ceruloplasmin 1356 However, in HBV- or HCV-related liver diseases, no significant site-specific change in core-fucosylation of ceruloplasmin was observed between HCC and cirrhosis. 24799124 7 20 gly core-fucosylation 1538:1554 arg1 ceruloplasmin ceruloplasmin PUBTATOR ceruloplasmin 1356 However, in HBV- or HCV-related liver diseases, no significant site-specific change in core-fucosylation of ceruloplasmin was observed between HCC and cirrhosis. 8381072 10 52 gly N-glycosylation 1898:1912 arg1 rat CGRP receptors rat CGRP receptors PUBTATOR CGRP receptors 24241 In conclusion, rat CGRP receptors with tissue-specific N-glycosylation but indistinguishable protein molecular mass have been identified in the cerebellum, brainstem, spinal cord, liver, and spleen. 9680123 0 43 gly glycoprotein 20:31 arg1 glycoprotein B glycoprotein B Cterm glycoprotein B Characterization of glycoprotein B of the gammaherpesvirus equine herpesvirus-2. 7964612 4 119 gly glycosylated 722:733 arg1 M protein form of M protein OGER form of M protein P54296 In addition, only the glycosylated form of M protein is secreted in contrast to both glycosylated and unglycosylated forms of L and S proteins that are secreted. 15504336 6 28 gly glycosylated 975:986 arg1 unexpectedly glycosylated ScFv unexpectedly glycosylated ScFv PUBTATOR ScFv 652070 When the first loxP site was used in the exon as a linker between ScFv and receptor, enhanced antigen-mediated cell proliferation and production of unexpectedly glycosylated ScFv were achieved. 27933781 1 7 gly glycoproteins 150:162 arg1 E1 E1 Cterm E1 The hepatitis C virus (HCV) envelope glycoproteins E1 and E2 are critical in viral attachment and cell fusion, and studies of these proteins may provide valuable insights into their potential uses in vaccines and antiviral strategies. 23305235 14 1 gly unglycosylated 1391:1404 arg1 Both glycosylated and unglycosylated GDNF Both glycosylated and unglycosylated GDNF PUBTATOR GDNF 2668 Both glycosylated and unglycosylated GDNF from mammalian cells are more stable than GDNF from E. coli. 23305235 14 18 gly glycosylated 1374:1385 arg1 Both glycosylated and unglycosylated GDNF Both glycosylated and unglycosylated GDNF PUBTATOR GDNF 2668 Both glycosylated and unglycosylated GDNF from mammalian cells are more stable than GDNF from E. coli. 11706042 1 24 gly multi-protein 141:153 arg1 The lipopolysaccharide (LPS) receptor multi The lipopolysaccharide (LPS) receptor OGER multi The lipopolysaccharide (LPS) receptor is a multi-protein complex that consists of at least three proteins, CD14, TLR4, and MD-2. 19038966 3 66 gly glycosylated 449:460 arg1 the fully glycosylated TPP1 precursor the fully glycosylated TPP1 precursor PUBTATOR TPP1 precursor 1200 We crystallized the fully glycosylated TPP1 precursor under conditions that implied partial autocatalytic cleavage between the prosegment and the catalytic domain. 10734111 2 70 gly modified 323:330 arg3 Notch1 AND O-linked glucose saccharides Notch1 O-linked glucose saccharides PUBTATOR Notch1 100761880 Here we show that Notch1 endogenously expressed in Chinese hamster ovary cells is modified with O-linked fucose and O-linked glucose saccharides, two unusual forms of O-linked glycosylation found on epidermal growth factor-like (EGF) modules. 10734111 2 70 gly modified 323:330 arg3 Notch1 AND O-linked fucose Notch1 O-linked fucose PUBTATOR Notch1 100761880 Here we show that Notch1 endogenously expressed in Chinese hamster ovary cells is modified with O-linked fucose and O-linked glucose saccharides, two unusual forms of O-linked glycosylation found on epidermal growth factor-like (EGF) modules. 19915009 3 67 part_of gp130 472:476 arg1 the extracellular domains D1-D3 gp130 the extracellular domains D1-D3 PUBTATOR SiteSequence gp130 3572 domains D1-D3 Whereas N-glycosylation of the extracellular domains D1-D3 of gp130 has been shown to be dispensable for binding of the gp130 ligand IL-6 and its cognate receptor in vitro, the role of the N-linked glycans on domains D4 and D6 is still unclear. 26354950 6 17 gly asialoglycoprotein 1212:1229 arg1 asialoglycoprotein receptor 1 asialoglycoprotein receptor 1 OGER asialoglycoprotein receptor 1 P34927 Examination of the pharmacokinetics of FST-ΔHBS-Fc molecules containing variable sialic acid content in asialoglycoprotein receptor 1 (ASPGR-1) knockout mice supports the receptor's role as part of the clearance mechanism of the molecules. 16510764 12 61 gly O-glycosylation 1499:1513 arg1 IgAN IgAN PUBTATOR IgAN 60498 These observations show that abnormal IgA1 O-glycosylation in IgAN is not due to an inherent defect in glycosylation mechanisms but arises only at a later stage in B cell development and may be secondary to aberrant immunoregulation. 30052682 0 23 gly glycoprotein 89:100 arg1 glycoprotein B glycoprotein B Cterm glycoprotein B 79594 Epstein-Barr virus activates F-box protein FBXO2 to limit viral infectivity by targeting glycoprotein B for degradation. 17714731 8 45 gly IgG 1529:1531 arg1 customized glycan profiles IgG customized glycan profiles Cterm IgG Thus, combination of the two endoglycosidases can provide a simple means of glycan analysis of both Fab and Fc by ESI-MS, which may contribute to the development of therapeutic IgG with customized glycan profiles. 1445902 4 42 gly deglycosylated 717:730 arg1 glycosylated and deglycosylated IL-3 glycosylated and deglycosylated IL-3 PUBTATOR IL-3 16187 Liquid secondary ion mass spectrometric analysis was carried out on the reduced tryptic and endopeptidase lysyl-C peptides of glycosylated and deglycosylated IL-3. 1445902 4 66 gly glycosylated 700:711 arg1 glycosylated and deglycosylated IL-3 glycosylated and deglycosylated IL-3 PUBTATOR IL-3 16187 Liquid secondary ion mass spectrometric analysis was carried out on the reduced tryptic and endopeptidase lysyl-C peptides of glycosylated and deglycosylated IL-3. 3031084 1 119 gly glycoproteins 234:246 arg1 Ribophorins I and II Ribophorins I and II PUBTATOR Ribophorins I and II 25596 Ribophorins I and II are two transmembrane glycoproteins that are characteristic of the rough endoplasmic reticulum and are thought to be part of the apparatus that affects the co-translational translocation of polypeptides synthesized on membrane-bound polysomes. 26328495 2 14 gly modified 392:399 arg3 POMGNT1 AND O-glycans POMGNT1 O-glycans PUBTATOR POMGNT1 55624 POMGNT1 is not modified by N-glycans because there are no potential N-glycosylation sites; however, it is not clear whether POMGNT1 is modified by O-glycans. 26328495 2 62 gly modified 272:279 arg1 POMGNT1 AND N-glycans POMGNT1 N-glycans PUBTATOR POMGNT1 55624 POMGNT1 is not modified by N-glycans because there are no potential N-glycosylation sites; however, it is not clear whether POMGNT1 is modified by O-glycans. 1377122 5 39 gly glycosylated 794:805 arg1 PC12 IGFBP PC12 IGFBP PUBTATOR PC12 IGFBP 24484 We showed that PC12 IGFBP is cationic, not glycosylated, with 25,500 mol wt reduced (18,500 unreduced), with high affinity for IGF-II and low affinity for IGF-I. 10801876 0 78 gly chain 25:29 arg1 human epidermal growth factor receptor epidermal growth factor receptor chain PUBTATOR epidermal growth factor receptor 1956 The Asn-420-linked sugar chain in human epidermal growth factor receptor suppresses ligand-independent spontaneous oligomerization. 9228058 7 36 part_of FasL 1238:1241 arg1 Tyr-218 FasL Tyr-218 PUBTATOR SpecificSite FasL 356 Tyr-218 Although the cytotoxic activity of mutant Y218D was unaltered, mutant Y218R was inactive, correlating with the prediction that Tyr-218 of FasL interacts with a cluster of three basic amino acid side chains of Fas. 11159927 4 20 gly glycosylation 479:491 arg1 IgG1-Pro-5 IgG1 PUBTATOR SpecificSite IgG1 105243590 The glycosylation profiles of IgG1-Lec 2 and IgG1-Pro-5 were heterogeneous. 2598815 0 51 gly glycosylation 25:37 arg1 N-CAM N-CAM OGER CAM P0DP23 Tissue specific O-linked glycosylation of the neural cell adhesion molecule (N-CAM). 30134158 1 13 gly glycoprotein 243:254 arg1 envelope glycoprotein envelope glycoprotein PUBTATOR envelope glycoprotein 155971 Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes. 30134158 1 13 gly glycoprotein 243:254 arg1 Env Env PUBTATOR Env 155971 Many broadly neutralizing antibodies (bnAbs) against HIV-1 recognize and/or penetrate the glycan shield on native, virion-associated envelope glycoprotein (Env) spikes. 11410585 4 34 gly present 798:804 arg1 bovine peripheral myelin P0 AND the HNK-1 epitope bovine peripheral myelin P0 the HNK-1 epitope Cterm P0 We recently described the structure of the N-glycan carrying the HNK-1 epitope, present on bovine peripheral myelin P0 (Voshol, H., van Zuylen, C. W. E. M., Orberger, G., Vliegenthart, J. F. G., and Schachner, M. (1996) J. Biol. 19951703 6 9 gly unglycosylated 826:839 arg1 unglycosylated UGT1A9 unglycosylated UGT1A9 PUBTATOR UGT1A9 54600 To evaluate the role of glycosylation in the enzyme activity, we produced unglycosylated UGT1A9 by treating HEK293 cells transiently transfected with expression plasmid with tunicamycin. 12063277 5 10 gly glycosylation 746:758 arg1 functional HERG channels functional HERG channels PUBTATOR HERG channels 3757 Our results show that N598 is the only site for N-linked glycosylation and that glycosylation is not required for the cell surface expression of functional HERG channels. 9587405 8 56 part_of Ser-52 2045:2050 arg1 factor VII/VIIa factor VII Ser-52 OGER SpecificSite factor VII P08709 Ser-52 and Ser-60 These findings strongly suggest that glycosyl moieties attached to Ser-52 and Ser-60 in factor VII/VIIa provide unique structural elements that are important for the rapid association of factor VII/VIIa with its cellular receptor and cofactor. 19808681 6 21 gly N-glycosylation 733:747 arg1 Kv12.2 Kv12.2 PUBTATOR Kv12.2 23416 As an effect of N-glycosylation on the function of Kv12.2, we demonstrate that removal of sugar chains causes a depolarizing shift in the steady-state activation without a significant reduction in current amplitude. 12356334 5 10 gly glycosylation 780:792 arg1 the SP-B variant the SP-B variant PUBTATOR SP-B variant 6439 The Thr131-->Ile substitution can eliminate a potential N-linked glycosylation site, Asn129-Gln-Thr131, which is present in the SP-B variant of the C allele (ACT/Thr) but not in that of the T allele (ATT/Ile). 10092871 3 71 gly unglycosylated 456:469 arg1 MMP-1 MMP-1 PUBTATOR MMP-1 4312 MMP-1 is secreted as both glycosylated and unglycosylated species, and the two forms have been shown to be identical with respect to substrate specificity, specific activity and inhibitory profile. 10092871 3 82 gly glycosylated 439:450 arg1 MMP-1 MMP-1 PUBTATOR MMP-1 4312 MMP-1 is secreted as both glycosylated and unglycosylated species, and the two forms have been shown to be identical with respect to substrate specificity, specific activity and inhibitory profile. 14693913 5 2 gly contain 868:874 arg1 hBSSL AND 16 Pro-rich 11-amino-acid repeats hBSSL The C-terminal 192 residues 16 Pro-rich 11-amino-acid repeats PUBTATOR hBSSL 1056 residues The C-terminal 192 residues of hBSSL contain 16 Pro-rich 11-amino-acid repeats, which include 32 Ser/Thr residues as potential O-glycosylation sites. 8892923 6 41 gly glycosylation 1225:1237 arg1 gp160 gp160 PUBTATOR gp160 2028 Deglycosylation experiments with three different glycosidases determined that the absence of gp120 was not due to aberrant glycosylation of gp160, indicating a defect in gp160 proteolytic processing. 1991473 2 0 gly N-glycosylated 268:281 arg1 hLH beta hLH beta PUBTATOR hLH beta 3972 hLH alpha (N-glycosylated at Asn52 and Asn78) and hLH beta (N-glycosylated at Asn30). 1991473 2 11 gly N-glycosylated 219:232 arg1 hLH alpha hLH alpha PUBTATOR hLH alpha 1081 hLH alpha (N-glycosylated at Asn52 and Asn78) and hLH beta (N-glycosylated at Asn30). 12171601 1 4 gly glycosylation 150:162 arg1 human proteinase-activated receptor-2 human proteinase-activated receptor-2 PUBTATOR proteinase-activated receptor-2 2150 We have analysed the role of N-linked glycosylation in regulating human proteinase-activated receptor-2 (hPAR(2)) expression and function. 12171601 1 4 gly glycosylation 150:162 arg1 hPAR(2) hPAR(2) PUBTATOR hPAR(2) 2150 We have analysed the role of N-linked glycosylation in regulating human proteinase-activated receptor-2 (hPAR(2)) expression and function. 9442070 12 117 gly N-glycans 1984:1992 arg1 IgA1 Fc IgA1 Fc N-glycans OGER IgA1 Fc P01876 As a consequence of both the primary sequence and S-S bond pattern, the N-glycans in IgA1 Fc are not confined within the inter-α-chain space. 12637583 4 15 gly regions 542:548 arg1 the heavy (H) chains chains regions OGER chains P11912 The O-glycan regions on the heavy (H) chains and the SC N-glycans have adhesin-binding glycan epitopes including galactose-linked beta1-4 and beta1-3 to GlcNAc, fucose-linked alpha1-3 and alpha1-4 to GlcNAc and alpha1-2 to galactose, and alpha2-3 and alpha2-6-linked sialic acids. 1324936 1 34 gly glycoprotein 139:150 arg1 Insulin receptor Insulin receptor PUBTATOR Insulin receptor 100766818 Insulin receptor (IR) is a glycoprotein possessing N-linked oligosaccharide side chains on both alpha and beta subunits. 1324936 1 44 gly possessing 152:161 arg1 Insulin receptor AND N-linked oligosaccharide side chains Insulin receptor N-linked oligosaccharide side chains PUBTATOR Insulin receptor 100766818 Insulin receptor (IR) is a glycoprotein possessing N-linked oligosaccharide side chains on both alpha and beta subunits. 29408166 8 91 gly core-fucosylated 1553:1568 arg1 core-fucosylated PSA core-fucosylated PSA PUBTATOR PSA 354 To our knowledge, this is the first MS-based method for quantification of core-fucosylated PSA in the low ng/ml concentration range in human serum. 23488770 4 12 gly glycoforms 653:662 arg1 IgG1 wt glycoforms IgG1 wt glycoforms PUBTATOR IgG1 16017 When using NS-0 and J558L cells for permanent transfection, IgG1 wt glycoforms differed between the two cell lines, whilst IgG3 wt glycoforms did not. 23488770 4 61 gly glycoforms 716:725 arg1 IgG3 wt glycoforms IgG3 wt glycoforms PUBTATOR IgG3 380795 When using NS-0 and J558L cells for permanent transfection, IgG1 wt glycoforms differed between the two cell lines, whilst IgG3 wt glycoforms did not. 2341397 0 14 gly glycoforms 57:66 arg1 nonspecific cross-reacting antigen nonspecific cross-reacting antigen PUBTATOR nonspecific cross-reacting antigen 1084 Sequence and glycosylation site identity of two distinct glycoforms of nonspecific cross-reacting antigen as demonstrated by sequence analysis and fast atom bombardment mass spectrometry. 23801331 0 3 gly acid 11:14 arg1 neuropilin-2 neuropilin-2 acid PUBTATOR neuropilin-2 18187 Polysialic acid on neuropilin-2 is exclusively synthesized by the polysialyltransferase ST8SiaIV and attached to mucin-type o-glycans located between the b2 and c domain. 28370891 16 55 gly ADAMTS-13 2194:2202 arg1 the N-linked glycans ADAMTS-13 the N-linked glycans PUBTATOR ADAMTS-13 11093 Conclusions Together, these data demonstrate that the N-linked glycans of ADAMTS-13 play a crucial role in regulating ADAMTS-13 activity. 22628310 4 3 gly glycosylation 884:896 arg1 Tmem27 trafficking Tmem27 trafficking PUBTATOR Tmem27 57393 Using Tmem27 mutational analysis and multiple biochemical approaches, we here show that Tmem27 dimerization is a dynamic process mediated by its intracellular cysteine residue and that prevents Tmem27 cleavage, that extracellular asparagine glycosylation is essential for Tmem27 trafficking to the PM and its processing by Bace2, that the amount of Tmem27 at the PM is proportional to its total cell levels upon glucose stimulation and Bace2 inhibition, and that the double phenylalanine motif in the Tmem27 cleavage site is an intramolecular Bace2 inhibitor. 10026267 2 46 gly glycosylation 225:237 arg1 hCTR function hCTR function Cterm hCTR P30988 The role of glycosylation in hCTR function has not been identified, but it has been suggested that inhibition of glycosylation does not affect binding or signaling. 9099677 9 10 gly Deglycosylation 1345:1359 arg1 the sFLT-1 the sFLT-1 OGER FLT-1 Deglycosylation of the sFLT-1(3), (4), (5), and (7) did not abolish VEGF binding. 8499451 0 53 gly glycosylated 14:25 arg1 glycosylated and nonglycosylated human transferrin glycosylated and nonglycosylated human transferrin PUBTATOR transferrin 7018 Expression of glycosylated and nonglycosylated human transferrin in mammalian cells. 8499451 0 71 gly nonglycosylated 31:45 arg1 glycosylated and nonglycosylated human transferrin glycosylated and nonglycosylated human transferrin PUBTATOR transferrin 7018 Expression of glycosylated and nonglycosylated human transferrin in mammalian cells. 8961954 11 72 gly glycosylation 1740:1752 arg1 the hPTH/PTHrP receptor the hPTH/PTHrP receptor PUBTATOR PTHrP receptor 5744 Our findings indicate that glycosylation of the hPTH/PTHrP receptor is not essential for its effective expression on the plasma membrane or for the binding of ligands known to interact with the native receptor. 22067045 10 106 gly O-glycoforms 1628:1639 arg1 structural isomeric IgA1 O-glycoforms structural isomeric IgA1 O-glycoforms PUBTATOR IgA1 3493 These findings represent the first definitive identification of structural isomeric IgA1 O-glycoforms, define the single-site heterogeneity for all O-glycan sites in a single sample, and have implications for defining epitopes based on clustered O-glycan variability. 25425657 2 61 gly α2,6-sialylated 416:430 arg1 α2,6-sialylated transferrin α2,6-sialylated transferrin OGER α2,6-sialylated transferrin P02787 Here we report that SSA inhibition is applicable to immunohistochemistry, localizing α2,6-sialylated transferrin in the liver. 22781125 3 5 gly O-glycosylation 794:808 arg1 mammalian α-DG mammalian α-DG Cterm α-DG To date, several studies have reported novel O-glycans and attachment sites on the mucin-like domain of mammalian α-DG with both similar and contradicting glycosylation patterns, indicating the species-specific O-glycosylation of mammalian α-DG. 29793953 6 5 gly glycosylated 1173:1184 arg1 Tagged NTCP Tagged NTCP PUBTATOR Tagged NTCP 6554 Tagged NTCP introduced to both HepG2 and HepaRG cells was glycosylated, with N5 and N11 being sites of N-linked glycosylation. 8747278 8 6 gly N-glycosylation 1419:1433 arg1 the NR1/NR2A receptor the NR1/NR2A receptor PUBTATOR NR2A receptor 2903 Furthermore, they suggest that N-glycosylation may be important for the correct formation of the channel domain of the NR1/NR2A receptor. 9790679 1 30 gly contains 199:206 arg1 DNase I AND mannose-phosphorylated oligosaccharides DNase I mannose-phosphorylated oligosaccharides OGER DNase I P24855 DNase I isolated from human urine (hDNase) or expressed in Chinese hamster ovary (CHO) cells contains mannose-phosphorylated oligosaccharides. 29249667 0 8 gly O-GlcNAc 0:7 arg1 PKCζ PKCζ O-GlcNAc PUBTATOR PKCζ 5590 O-GlcNAc on PKCζ Inhibits the FGF4-PKCζ-MEK-ERK1/2 Pathway via Inhibition of PKCζ Phosphorylation in Mouse Embryonic Stem Cells. 17222411 0 29 gly glycosylation 11:23 arg1 human podoplanin human podoplanin OGER podoplanin Q86YL7 Functional glycosylation of human podoplanin: glycan structure of platelet aggregation-inducing factor. 2524188 1 2 gly Aglycosylated 155:167 arg1 Aglycosylated human IgG1 Aglycosylated human IgG1 OGER IgG1 P01857 Aglycosylated human IgG1 and IgG3 monoclonal anti-D (Rh) and human IgG1 and IgG3 chimaeric anti-5-iodo-4-hydroxy-3-nitrophenacetyl (anti-NIP) monoclonal antibodies produced in the presence of tunicamycin have been compared with the native glycosylated proteins with respect to recognition by human Fc gamma RI and/or Fc gamma RII receptors on U937, Daudi or K562 cells. 11695756 1 36 gly glycoprotein 98:109 arg1 Dentin sialoprotein Dentin sialoprotein PUBTATOR Dentin sialoprotein 25254 Dentin sialoprotein (DSP) is a major glycoprotein present in the mineralized dentin matrix that is expressed mainly by young and mature odontoblasts. 6853480 0 40 gly glycosylation 35:47 arg1 human serum albumin human serum albumin OGER albumin P02768 The principal site of nonenzymatic glycosylation of human serum albumin in vivo. 15955802 6 4 gly contain 609:615 arg1 IgM AND 23.4% oligomannose glycans GlcNAc2Man5-9 IgM 23.4% oligomannose glycans GlcNAc2Man5-9 OGER IgM P01871 IgM was found to contain 23.4% oligomannose glycans GlcNAc2Man5-9, consistent with 100% occupancy of Asn-402 and 17% occupancy of the variably occupied site at Asn-563. 26578555 8 5 gly SugarBindDB 956:966 arg1 a glycan-lectin binding pair SugarBindDB a glycan-lectin binding pair Cterm SugarBindDB Thus, a glycan-lectin binding pair of SugarBindDB can lead to the identification of a glycan-mediated protein-protein interaction, that is, a lectin-glycoprotein interaction, via substructure search and the knowledge of site-specific glycosylation stored in UniCarbKB. 2537322 1 96 gly glycoprotein 228:239 arg1 The leukocyte function-associated molecule 1 The leukocyte function-associated molecule 1 PUBTATOR leukocyte function-associated molecule 1 3683 The leukocyte function-associated molecule 1 (LFA-1, CD11a/CD18) is a membrane glycoprotein which functions in cell-cell adhesion by heterophilic interaction with intercellular adhesion molecule 1 (ICAM-1). 15322230 3 48 gly P-glycoprotein 343:356 arg1 P-glycoprotein P-glycoprotein PUBTATOR P-glycoprotein 5243 We found that P-glycoprotein was constitutively ubiquitinated in drug-resistant cancer cells. 10603327 1 29 gly glycoprotein 211:222 arg1 sGP sGP Cterm sGP In the present study we have investigated processing and maturation of the nonstructural small glycoprotein (sGP) of Ebola virus. 18931413 3 50 gly glycosylation 399:411 arg1 mammalian IgGs mammalian IgGs Cterm IgGs The N-linked glycosylation at Asn297 is conserved in mammalian IgGs as well as in homologous regions of other antibody isotypes. 23891555 21 94 gly O-glycopeptides 3372:3386 arg1 IgA1 IgA1 OGER IgA1 P01876 The novelty and biological significance reside in the demonstration, for the first time, of the distribution of the most abundant isoforms of HR O-glycopeptides of IgA1. 15448157 6 66 gly glycosylation 812:824 arg1 PrP PrP PUBTATOR PrP 19122 However, in earlier studies, mutations at the glycosylation sites of PrP led to intracellular trafficking abnormalities, which made it impossible to generate PrP glycosylation-deficient mice that were susceptible to bovine spongiform encephalopathy (BSE) or scrapie. 2605214 0 16 gly Lol 86:88 arg1 a Lolium perenne (perennial rye grass) pollen allergen Lol p III a Lolium perenne (perennial rye grass) pollen allergen OGER Lol p III Q08397 Complete primary structure of a Lolium perenne (perennial rye grass) pollen allergen, Lol p III: comparison with known Lol p I and II sequences. 24337809 0 50 gly glycosylation 9:21 arg1 the bone morphogenetic protein receptor type 2 the bone morphogenetic protein receptor type 2 PUBTATOR bone morphogenetic protein receptor type 2 659 N-linked glycosylation of the bone morphogenetic protein receptor type 2 (BMPR2) enhances ligand binding. 24337809 0 50 gly glycosylation 9:21 arg1 BMPR2 BMPR2 PUBTATOR BMPR2 659 N-linked glycosylation of the bone morphogenetic protein receptor type 2 (BMPR2) enhances ligand binding. 7584863 0 56 gly glycoprotein 69:80 arg1 MAG MAG PUBTATOR MAG 4099 Conservation of functionally important epitopes on myelin associated glycoprotein (MAG). 7584863 0 56 gly glycoprotein 69:80 arg1 myelin associated glycoprotein myelin associated glycoprotein PUBTATOR myelin associated glycoprotein 4099 Conservation of functionally important epitopes on myelin associated glycoprotein (MAG). 12228891 3 71 gly AGP 640:642 arg1 the oligosaccharide chains AGP the oligosaccharide chains Cterm AGP The APR also results in alterations to the branching, sialylation and fucosylation of the oligosaccharide chains of AGP. 1705276 1 2 gly microheterogeneity 201:218 arg1 AFP AFP PUBTATOR AFP 174 By means of lectin affinity crossed-line immunoelectrophoresis, microheterogeneity of alpha-fetoprotein (AFP) was studied in 41 amniotic fluid samples between 41 to 287 days of gestation. 1705276 1 2 gly microheterogeneity 201:218 arg1 alpha-fetoprotein alpha-fetoprotein PUBTATOR alpha-fetoprotein 174 By means of lectin affinity crossed-line immunoelectrophoresis, microheterogeneity of alpha-fetoprotein (AFP) was studied in 41 amniotic fluid samples between 41 to 287 days of gestation. 27550041 1 2 gly lactoferrin 181:191 arg1 the N-glycans lactoferrin the N-glycans PUBTATOR lactoferrin 100861194 A comprehensive monosaccharide composition of the N-glycans of donkey milk lactoferrin, isolated by ion exchange chromatography from an individual milk sample, was obtained by means of chymotryptic digestion, TiO2 and HILIC enrichment, reversed-phase high-performance liquid chromatography, electrospray mass spectrometry, and high collision dissociation fragmentation. 11903056 0 84 gly glycoprotein 45:56 arg1 the 5T4 glycoprotein oncofoetal antigen the 5T4 glycoprotein oncofoetal antigen PUBTATOR 5T4 glycoprotein 7162 Glycosylation and epitope mapping of the 5T4 glycoprotein oncofoetal antigen. 1370171 1 63 gly glycoprotein 123:134 arg1 CD34 CD34 PUBTATOR CD34 947 CD34 is a 115-kDa transmembrane glycoprotein of unknown function that is expressed on human hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. 20030399 8 70 gly glycopeptides 1293:1305 arg1 human secretory IgA IgA IgA P11912 The application of this tool to characterize the N- and O-linked glycopeptides from human secretory IgA (sIgA), consisting of secretory component (7 N-linked sites), IgA1 (2 N-linked,